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What were your first opinions of the characters?
, This shall be interesting...
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Oct 4 2009, 8:22 PM
Believix Fairy
Posts: 9,391
Joined: February 22 2009
From: Way up high
Alright, this is going to be an epic topic. What were your first opinions of the characters? Who did you like? Who did you hate? Who'd you think was creepy?
Here be mine. Mind you, I was 10 when Winx Club came out...:
Bloom: I've never really been too fond of her. Her personality is far too...let's say loud, for me.
Stella: This girl is absolutely amazing. I've always loved Stella.
Flora: *Puts bag over head* I used to adore Flora. She was my fave for at least half of season 1, until I realized how cool Musa was.
Tecna: She was alright. Originally, I thought her character was kinda bland, and boring. But now she's my 3rd fave Winx girl, so it's all good.
Musa: She's always been one of my faves. At first, she was my 2nd fave or so. I think I felt bad for her or something after Riven was a spaz, and grew to like her more after that.
Sky: I've always had a place in my heart for Sky. Yes, I know most people are annoyed with him, but I found him absolutely charming, and I still think that.
Brandon: One word: Epic. Brandon was my fave at the beginning. He's always been absolutely hilarious, and whatever it is about his character that makes him extremely likeable.
Riven: I couldn't stand the dude. Yes, the truth comes out. I hated Riven, and I'm not afraid to say it. The Self-Proclaimed Riven Expert hated Riven folks. He was such a jerk in season 1! Eesh! God, I don't even want to get into how much I hated him.
Timmy: He was pretty flat sided in season 1, so I found Timmy pretty boring.
Helia: I've never liked him. That's all I will say. I'm not in the mood to rant. XD
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Oct 4 2009, 8:30 PM
Believix Fairy
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From: wilmington, delaware
Bloom: Thought she was down to earth, but seemed naive to me for some reason
Stella: She was the stereotypical fashion girl although i did find her amusing and she became my favorite right off the bat
Flora: I didnt expect her to be a quiet, hippie type girl
Tecna: Didnt like techna her voice annoyed me and so did her outfits, but she kinda grew on me
Musa: Her voice DEFINATELY annoyed me, and really didnt like her and to this day i still dont like her, shes to dramatic for me
Sky: dont like him, and still dont like him
Brandon: seemed like a surfer dude type person
Riven: thought he was a douchebag, and he really was lol, i swore him and bloom would have hooked up but they didnt.
Timmy: thought he was a typical nerd
Helia: liked him right off the bat, i really digged his hair
This post has been edited by
: Oct 4 2009, 8:34 PM
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Oct 4 2009, 8:31 PM
Believix Fairy
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Joined: February 22 2009
From: Way up high
Terri, you weren't 10! Lol.
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Oct 4 2009, 8:32 PM
Believix Fairy
Posts: 81,690
Joined: April 10 2007
From: England
to be honest i cannot remember too well, inncluded latter characters as well
bloom: i like her
stella: really liked her
flora: cool
musa: not too good
tecna: ok
layla: not great
roxy: not good
brandon: ok
sky: good
nabu: weird
icy: ok
darcy: ok
stormy: ok
darkar: good
baltor: great
oregon: great
anagon: annoying
dumos: annoying
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Oct 4 2009, 8:32 PM
Believix Fairy
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For the most I can't really tell except for the recent additions. Only Stella, because around 'The day of the rose' episode, she got a major opinion overhaul. She went from *Sigh* yet another fashion-obsessed blonde, to one of my favorite Winx girls. (It's the way she talked about her parent's divorce. As a girl with divorced parents, the sad, frustrated but knowing you can't help it kind of felt familiar.)
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Oct 4 2009, 8:35 PM
Believix Fairy
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Joined: February 9 2008
From: wilmington, delaware
QUOTE (winxclubrox23 @ Oct 4 2009, 04:31 PM)
Terri, you weren't 10! Lol.
lmao...no i wasnt 10
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Oct 4 2009, 8:51 PM
Believix Fairy
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From: somewhere over the raiinbow
Okay, here's mine. I dont remember much but this is probably somewhat accurate.
Bloom: Like alot of main characters, she seemed average when it comes to most things [except power, obviously] and she was always near the bottom of my list though I did find her amusing.
Stella: She's hilarious, and I loved how orange was her colour. And then in the very first episode was her winx transformation, which has absolutely EPIC music in 4kids, and she was an instant near the top of the list.
Flora: I think she was originally my fave but then came second to Stella a little into season 1, I've always liked Flora.
Tecna: She was pretty medium for me, originally 4th in my list. Her appearance isnt too great but I really like her powers.
Musa: I've always liked Musa, her powers especially. And how she like raps and stuff in the middle of episodes. She was another top contender, but in the end I settled with Stella, Flora, Musa in that order and have stuck with it since.
Layla: She seemed really interesting, but never broke the top 3.
Sky: Never liked him, he looks stupid. And believe it or not, I used to hate the name Brandon cause of this stupid kid I knew with the same name. Then when they switched I somehow started really liking the name lol. [Oh and the kid moved before they switched so its all good xD]
Brandon: Awesome. He's been my favourite character since the beginning, I love his personality and all.
Riven: I'm pretty sure I used to hate him lol.
Timmy: Cant really remember, I dont think I liked him much.
Helia: When I first saw him, I'm like uh oh, cause they were hinting Flora would get someone that eppy and then when it happened, I almost cried [okay no I didnt but still i was disappointed lol]
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Oct 4 2009, 9:26 PM
Believix Fairy
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From: Here.
Bloom - Too perfect and not realistic enough.
Stella - Drama queen and had the attitude
Flora - Sweet and quiet. My favorite and still is.
Tecna - Bossy?
Musa - A party girl and a tomboy. I really hated her voice in the 4kids version and found her hair very funky. XD But I started to like her more in the 3rd season. Now I consider her to be one of my tops.
Layla - A sporty chick with attitude. I like her independent attitude. I liked her. But when Nabu came along...I kind of started to forget about her. O_O
Sky - No opinion. He was just there to me. Though I had to saw he was kind of annoying..
Brandon - LOVED HIM AND STILL DO!
Riven - I have to say that I like him better in my imagination and fanfics than when I first met him. He was a jerk. I usually had a tendency to like jerks though because I thought they would eventually become good so I kind of had a love-hate feeling. :)
Timmy - I kind of treated him as a wallflower and I feel bad about it. O_O
Helia - At first I was kind of weirded out by his long hair because he looked like a girl. I started to like him more as the series went on but I still kind of think he needs more attitude. I have to admit that part of the reason that I like him is because he is with Flora. ><
Overall - When I first met all the characters.....I kind of laughed at their voices. O_O Their 4kids voices seemed funny to me. O_O I feel guilty about it but I couldn't help it. Luckily I eventually started paying attention to actual plot. :)
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Oct 5 2009, 3:35 AM
Believix Fairy
Posts: 9,391
Joined: February 22 2009
From: Way up high
QUOTE (Mazei AD @ Oct 4 2009, 02:25 PM)
Riven: He was such a jerk! I didn't like him. I used to hope he died sometime in the season
Die!?! Ouch! I didn't hate him that much! Lol.
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Oct 5 2009, 11:43 AM
Believix Fairy
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From: South Africa, Durban
Good topic idea...blah, I'll have to fill in this later >.>
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Oct 6 2009, 1:55 AM
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From: Washington USA
Bloom: My fave charecter ! !!!!!she is cool
Stella: she
to be my fave
Flora: uhhh ok to ummmm goody-goody
Musa: she is awsome
Tecna: i used to hate tecna now she is my 2nd fave chareceter
Sky: he's ok Bloom's boyfriend
Brandon: the BEST
Timmy: he's ok
Riven: at first i thought he was really stuck uo
Helia: uhhh no
Another Morning
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Oct 6 2009, 2:39 AM
Believix Fairy
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From: LOOK BEHIND YOU
I don't remember too much. All I remember is that for some reason, I thought Stella was supposed to be the main character (I think my SM background told me that moon powers = main character), and I didn't know that Tecna and Musa existed until I saw the show XD I'd only seen Bloom, Stella, and Flora beforehand.
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Oct 11 2009, 1:29 AM
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OK, well this is just from me watching, i think, the first episode they apeared in.
Bloom: To weak and such. But i grew to like her when she became more powerfull
Stella: Stella rocks!!! She started out as my favourite, because i really like her personality. She is still kinda my favourite now.
Flora: OK, i guess. She's just too quiet.
Musa: I couldn't say she was my favourite, but sometimes she could be pretty funny. I didn't like her at first because she never let her hair down(I was only 6 at the time) and that she liked Riven. The episode in season 3 when she put her hair down i was running around the house screaming and brought the doll for 60 bucks.
Tecna: I really loved her voice! She grew to be one of my favourite characters.
Layla: She was never anywhere near the top of my list. I hated in season 3 were she kept on going and visiting Andros/Tides and such. so anoying because i wanted to see them go back to Solaria.
Sky: He was so anoying. I hated everything about him. That was kinda the reason I disliked Bloom at the beginning.
Riven: I didnt really care about him. I just thought he was someone who gets in the way all the time.
Helia: In Season 2 and 3 he was cool, but season 4 when he got his haircut he just looked plain stupid. Seriously. One of the reasons I really liked Helia was because of his hair.
Timmy: Just plain anoying.
Brandon: He is perfect for stella. Definantly my favourite specialist.
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Oct 12 2009, 3:18 AM
Enchantix Fairy
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From: Australia
These were my VERY first opinions of the girls, they changed since then
Bloom: The leader of the gang but I though her weak
Stella: A typical blonde girl who likes shopping (I liked her (IMG:
) )
Flora: Quiet, sweet and girly
Musa: Loud and a tomboy (oppisite of Flora)
Tecna: Very sophisticated and smart
Layla: Sporty and not very social
Roxy: Brave but she still has a lot to learn
Specialists: The reason they are all under one title is because they were pretty much the same to me then. All brave, attractive and sweet.
This post has been edited by
: Oct 12 2009, 3:20 AM
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Oct 12 2009, 8:46 AM
Believix Fairy
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From: A Land Down Under
Bloom:I used to like her a lot and I thought of her as the weak-Usagi esque(well minus the ditzy), discovcering powers character.
Stella:SHopping. Obsessed. Blonde. Nuff said.
Musa:The musical, gangsta-girl try-hard(keep in mind this be the 4kids version. But then I realized I liked her)
Flora:Quiet and sugary sweet. A bit too much for my tastes at first.
Tecna:The smart, but slightly geeky brainiac girl(but geeks are cute xD).
Layla:SPorty and at first she came across at anti-social. O and of course feminisist
Specialists: TO me at first, they were the token guy characters who would assist the girls at certain times. I had slightly different ideas about each, but it was basically the same, just 'oh they've all got kinda conflicting personalities'.
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Dec 18 2009, 3:00 AM
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i was 8 when i first saw winx, keep it in mind!
Bloom - i hated her. i'm sorry, but i HATED her. still do.
Stella - ♥d her! my fave fairy right from the beginning. she's funny, fashion-obsessed (like me), and pretty
Flora - didnt really have an opinion of her, other than that she was pretty
Tecna - funny, she seemed kinda weird
Musa - i didn't like her, she was too up-in-your-face
Icy - I WAS OBSESSED WITH HER!!! my first thought was "omg she is SO pretty!" and i fell in love with her powers, her comebacks, her plans, etc.!
Darcy - i thought she was cool (people began making me dislike her recently because there all "oh she's soo much better than Icy" but if i ignore other people's opinions, i still think Darcy's cool)
Stormy - she seemed weird and cool to me at the same time ha
Sky - i didnt like him cuz he was Bloom's bf lol. and also cuz he left Diaspro! (let's drop the Diaspro topic there, tho, i dont feel like starting something)
Brandon - he was cute! hehe! i thought he was cool and romantic and perfect for Stella
Riven - i thought he was kinda cool, the rebelious type. yeah he was a jerk but he made the episodes interesting (i still laugh every time at the one part of 'Grounded' :
So, do you like to dance Tecna?
Not really. I don't really know any dance steps.
I'll tell you the steps I know, step 1 DON'T dance ya get it?
Whoa, Timmy, keep fallign like that and we'll start calling you Tim-ber LOL!!!!!
Timmy - he was nerdy but he cracked me up!
Layla - didnt like her cuz i liked the original 5 Winx girls as a group
Darkar - i cannot describe my hatred for that...thing. He's so ugly & scary & tries to manipulate Icy...that crosses my line right there!
Pixies - i didnt like them, i mean the witches use to call the winx girls pixies to INSULT them! i mean REALLY!
V/Baltor - I. DID. NOT. LIKE. HIM. ugh....i just reeeeeeally didnt.
sry for posting twice, but i accidentally submitted b4 i meant to.
i dont really have an opinion there bcuz i havent watched it yet.
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Dec 18 2009, 3:27 AM
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Bloom:she's my fave because she's really friendly and powerful
Stella:she's a bit funny and fashion crazy most of the time
Flora:she's really sweet and kind
Layla:she's very energetic and independent
Tecna:she's really smart and is a bit of a computer freak
Musa:she's really into music but she ca n a bit bossy
Roxy:she's really loving and cares a lot about animals
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Apr 2 2010, 11:38 PM
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Bloom: Thought she was going to be the typical "newbie" like in every single one of those movies/shows about somebody new comming into a magic school. She seems rather plain to me, however her personality makes her a PRIMO target for TONS of appealshipping fanfictions, primarily because she's gullible and small minded when it comes to the realm of magix.
Stella: What I call a "bobble-head". She seems like a bit of a nobody to me, even though she is supposed to take over her kingdom. She also seems like a bit of a player, which mainly comes from my "bobble-head" deduction. Never liked her powers, or her personality, because it is really hard to simulate that kind of bobble-headed-ness in a fanfiction without making her seem completly stupid, or ADHD.
Flora: I am at a moot point with her. I don't like her all that much, though her and Bloom are UUBER cute. I don't hate her all that much, though in my opinion her powers leave a lot to be desired from a plant fairie.
Tecna: She was, at the time I saw the first season in 2004, the character I related to the most. I loved technology, especially video games. Her personality reminded me of my brothers, when taking apart a cellphone was basically his cocaine. Over the last few years I've begun to appreciate her personality as well as her powers. Her original WinX outfit alwase made me go "ew" because of the helmet. *so glad they got rid of the helmet*
Musa: Tomboy-ish, which drew me into her, as well as her powers. Some of them never made sense to me though. Her personality is fun to play with in a fanfic because she's a bit of a tomboy, which leaves little to be immagined along the fanfiction front
Sky: Oh, you mean Mr. Blonde, dumb and ugly? Hated him. Alwase hated him. Mainly because he was FAR to close to your typical fairy tale prince.
Brandon: He had a bit of a bland vibe to me, and he still does. Vary plain, and all around undesired.
Riven: At first thought he was kinda cool, because he was an emo. Now I've labeled him as a player, and want to tear his skin of for being with Musa, which can so CLEARLY do better.
Timmy: AHH! Nerd with a sword!! What's he gonna do? CODE me to death with that HTML junk I use on myspace? I saw him as a joke, and was SURE he would be killed off before the end of the first season.
Helia: three words: Giant. Walking. *****.
Icy: D*MN! She was epic! I LOVED her ice power, not to mention her hair! The first time I saw her hair I printed a picture of her off the net, showed it to my mom and told her I wanted to do this to my hair! She nearly passed out and I couldn't get my dad to stop laughing! However, as the years passed I started to dislike her because of her frequent outbursts.
Darcy: My most favoured character of the entire show. When I first saw her, and she had on her orange glasses, she reminded me of one of those wiered artistic, half-emo poet people you meet in college. She still does, however now I have quite a bit more respect for her, and a distinct hatred for the show because they refuse to develop her powers into what they could be. *cough cough* Darcy could take over the world *cough cough*
Stormy: I HATED her hair. And because of that I never really gave myself much of a chance to appreciate her character. Now that I don't really mind her hair, I ADORE her powers, as well as her temper to an extent. When I first saw her, she reminded me of an imp, giving smart remarks and snickering.
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Jun 12 2010, 9:21 PM
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From: Gardenia.
Bloom- As you can see she was my fave , but not now.. I guess I liked her , because she seemed and average normal person , who is kind and caring and handles the leader role very well , but when the show continued , she turned out to be kind of boring to me.
Stella- I have loved her always. She is a full of energy and a funny girl, She always made me laugh and love how she can be a diva , but still not just a beach babe, but a character that also has deep feelings and cares alot about her friends ans everyone.
Flora- This girl is made out of candy and sugar. Really loved her , because she seemed really pretty to me , like a flower princess :). But I also like her , she is very sweet and quiet. The opposite of Stella , who is quite loud.
Musa- She is my favourite ! Love her attitude, her way of talking , her power- MUSIC , personality. She can be dramatic , but she is a lot like me.Adore her tomboyish side too.
Tecna- She seemd pretty boring and plain bookworm , but she grew into me. Now I like her.
Layla- Well I love her dancing skills , she is okay.
Sky- When I was younger , he seemed okay, but now I really don't like him. I love her relationship with Bloom , but I don't like how he can be such a mamma's boy and I got seriously p*ssed when he lied to Bloom that he was Brandon and he had a girlfriend or a fiance already. I mean , it was one sided and only Diaspro thought that they were in love , but cmon if you love her , then you would do anything to make her happy and not wait until she finds out the truth herself.
Brandon- Love him! He is hillarious ladies man. Problaby the best looking too. But they are cute with Stella and love him , saying it again.
Helia- Like him too. I love romantic guys , I think it is very sweet if man can write a poem or something beautiful . swwweeeettt.
Riven- I soo love him ! I have always had a thing for bad boys, dunno why (IMG:
) . But I will be honest , that he seemed as a total jerk and is very problematic , but still deep down he is very caring , especially for Musa. It is his ego side that keeps him from being that.But I love him !
Timmy- I thought he was really cute and this cute geek type. Geeks and nerds are cute (IMG:
Nabu- Well , I miss him. In season three , he seemed fine , but in season four I began to love him. He is very strong and you can always feel safe with him , but also very loving and passionate and would do anthing for you.
The Trix- I really was a winx fan more at first , but now I really love them. They are evil , but so funny and they crack me up. I love Darcy the most , dunno why. The least Stormy, maybe because she outbursted for every little thing , I was like chill , girl (IMG:
) ´. But Icy is also awesome. I think the three of them , make the awesome group they are.
Darkar- Seemed nothing to me, he laughed a lot and seemed to me typical villian who wants power.
Valtor- Like him a lot. Love his smirk that he makes all the time and I used to wonder , what it could be if Bloom was with Valtor or something. But I wish he could be younger .
The black circle- I thought they were very cool :).
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Jun 13 2010, 10:34 PM
Believix Fairy
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Good idea for a thread! Kudos!
- I instantly caught on to the fact that she was the main character, and also instantly recognized the perfections that she was given. Although, at that time, she didn't really bother me all too much, for I didn't find her all that interesting (that and I did not know about the massive amount of screentime she had been given; I jumped in at the end of season one.)
- I thought she was a humorous and enjoyable character; I also caught on to her being a main(ish) character.
- Honestly, I didn't think much of her... I didn't really notice her, (most likely due to the lack of screentime she was given). To be honest, I don't even remember when I formed an opinion on her... for the longest time I just ignored her. o_O
- She actually was my favorite character because of her similarity in appearance to "Jade" (I liked Bratz at the time). I thought she was, simply, "cool".
- Like Flora, I didn't really notice her at first (again, lack of screentime). I do remember really liking her accent though. After a few more episodes, though, she quickly became my favorite character.
- When I first saw Layla, I was extremely excited about her introduction into the series (I absolutely loved her look and her Winx outfit).
(if she's officially labeled a Winx Club member)
- I'll be honest, I instantly did not like her. I didn't feel another Winx Club member was at all necessary.
- I instantly labeled him "Prince Charming"; I found it blatantly obvious that he was supposed to be the "perfect guy" character.
- I remember thinking Brandon was a good character; I had no problems with him.
- I honestly do not remember what I initially thought of him, it may have been a simple recognition of him being the "brains" of the specialists, but I honestly cannot recall.
- The only thing I remember about my initial opinion about Riven had something to do with his hair... :/ I think I saw it as an "interesting" hairstyle... or unusual... something like that...
- Initial opinion = A character thrown in there to give Flora a boyfriend.
(He's not really a specialist, but I'll throw him in this category anyway)
- The way he was introduced into the series was a bit... weird. I initially thought he was a creepy stalker.
- I think I initially thought she was pretty "cool". (IMG:
) (Excuse the bad pun)
- I do know that I thought she was a pretty character... Don't remember anything else, though.
- I instantly did not like her... at all. I cannot tell you why, I just... didn't.
- I thought he had some potential to be a really "bad" villain.... I was wrong.
- Like Darkar, I initially thought he had potential... again, I was wrong.
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Estimated revenue calculated due to the firm's first acquisition of five vessels for shipping bitumen shipping vessels is about $39 million annually and $6 million for EBITDA. When this is completed, the firm in collaboration with Network 1 and its expert team of advisors, will commence the acquisition of additional ships to increase its fleet all while with strategizing the lease or purchase of export and import facilities, distribution equipment, asphalt refineries, and associated businesses. The purpose of this to take advantage of projections that the total income for bitumen producing institutions will exceed $25 billion in 2018 while global bitumen revenue is estimated to get as high as $100 Billion by 2020 as the USA's expands its infrastructure process.
U.S. President Trump has previously suggested financing improvements using an estimated $200 billion in government funds in roughly a decade which would leverage about $1.0 trillion in waterfall construction dollars. At the same time, investment in infrastructure investment all over the world is still growing hence, the firm and its management are eager to be players in an industry that promises to remain lucrative.
Chief Executive Officer of Turner, Steve Helm outlined that besides going into the distribution and supply part of the bitumen market, the firm is also going into the shipping markets at a time where prices are ridiculously low with plans to implement a serious growth plan to increase from the starting five bitumen tankers to as high as thirty tanker ships within the next twelve to eighteen months by taking advantage of the investment banking relationship with Network 1.
The firm's leadership had already negotiated the transaction using preference shares, which are expected to give good returns to the investors while helping the firm from further diluting its ownership. It has been Turner's policy to avoid or at least reduce any share diluting activity. The deal is in line with the already announced purchase of five Bitumen Tanker Ships which were inspected and valued at more than $28 million and run rate much higher than its run rate of well over $14 million in income, which was done using preference shares as well.
Financial Performance
According to its statement of comprehensive income, the firm did not record any revenues in the last financial year. This continued the trend of no revenues for at least the last three years, a common feature of firms in the growth stage. There was also no recorded cost of sales or research and development costs in its books. The firm's sparse statement demonstrates the fact that the firm remains in a phase which does not bring in a lot of income.
The firm is highly leveraged as its liabilities are estimated to be worth no less than 15 times its assets and just as much as its assets. Its very low liquidity of 0.04, made more worrying the absence of any cash means the firm may be incapable of handling its regular operations smoothly over the year.
TVOG has a lot of promise, due to its strategic position in a burgeoning industry. However, the firm must begin to monitor its financial performance more closely to avoid future problems.
We will be updating our subscribers as soon as we know more. For the latest updates on TVOG, sign up below!
Disclosure: We have no position in TVOG and have not been compensated for this article.


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Retail wrap: Raising Cane's, Camp Bow Wow, My Salon Suite ink new deals
My Salon Suite opened a 4,882-square-foot location at 1507 W. 18th St. in Brixmor's newly renovated Jester Village shopping center west of the Heights. The location, which houses a collection of private suites for a variety of salon professionals, is among 10 Texas locations opened by franchisee Aaron Gillaspie. A Heights-area location is planned to open at 2200 Edwards St. across from Sawyer Yards by year end. … [Read more...] about Retail wrap: Raising Cane's, Camp Bow Wow, My Salon Suite ink new deals
Feb. 18 Is The Voter Registration Deadline For The March 3 Presidential Primary
Vote at a vote center. These centers are open from 8 a.m. to 5 p.m., Feb. 22 through March 2. Vote Centers on Election Day will be open 7 a.m. to 8 p.m. Vote by mail. With this option, you cast your votes on a ballot in the comfort of your own home, then mail or deliver it to a polling place by 8 p.m. Election Day. Click here to access the vote by mail application. Click here to access the electronic One-Time Vote by Mail Application or complete the Permanent Vote by Mail Application. Vote early. This option is available beginning 29 days before an election. Voters can come to the Norwalk main office to vote early. Two weeks before the election, the office is open Saturday and Sunday, from 8 a.m. to 4 p.m. For voter assistance in your language, don't hesitate to reach out to a toll-free Voter Hotline. … [Read more...] about Feb. 18 Is The Voter Registration Deadline For The March 3 Presidential Primary
LA Community Colleges To Host Early Voting Centers For California Primary
The voting centers are part of California Secretary of State Alex Padilla's initiative to encourage students to vote. The California Students Vote Project partners with most higher education institutions in the state to encourage student voter registration and provide easy access to polling locations. In 2016, the California Student Voter Registration Act passed, requiring every California community college and state university to allow its students to register to vote during class registration and other critical academic periods. The law also states that students must be able to register to vote through their college's web portal, via a link to the California Secretary of State online voter registration system. … [Read more...] about LA Community Colleges To Host Early Voting Centers For California Primary


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Due to my busy IRL work schedule and other commitments, I can no longer in good conscience accept donations for my game development, as I do not feel that I can keep up to a pace which meets the reasonable expectations of the people who have been kind enough to support my projects.
Please do not subscribe. I am continuing to develop my games as normal and will post future updates to thundorn.com
These links and the ones provided at the official site are the ONLY official downloads. If you acquire the files from anywhere else I accept no responsibility for any of the content.
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Archived posting to the Leica Users Group, 1998/10/04[Author Prev] [Author Next] [Thread Prev] [Thread Next] [Author Index] [Topic Index] [Home] [Search]
In a message dated 98-10-04 17:07:29 EDT, you write: << B&H makes a folding rubber hood in 39mm and a foldign wide angle hood (not in 39) has anyone used htese products or specifically has anyone used them with the new 50/2? >> - ------------------------------- Michael & Jim: Perhaps I missed the first part of this thread, but what is wrong with the built-in lens hood on the new 50f2.0?? I think the built-in lens hood on the Leica lenses that offer them is a tremendous attribute. Arturo


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This is a genuine 1966 Sunbeam Tiger that was first registered to the London Metropolitan Police on November 1st 1966. VIN Number: B382002380 HROFE, JAL 662385. Jensen Build Date 26th August 1966. JUL 390D is one of only 4 late MKIA Sunbeam Tigers acquired by London's Metropolitan Police in 1966. The Tiger went into service at the Hampton police station in Middlesex and during its brief 2 year service was widely used as a pursuit vehicle on the new London motorways (M4/A4) for catching speeding motorists. This one is a particularly nice example that has been owned for 15 years by the current owner. Much research was completed into the Metropolitan police Tigers during his ownership. The Tiger was reunited several times with Sergeant Police drivers formerly based at the Hampton police station. A large history file comes with the car that has a lot of old in period pictures, history, old MOT's and invoices etc. Fully restored and equipped with some original police equipment it has the Police radio, Winkworth bell and two tone siren fitted and working. It is said the Police sign was on the rear of the car so as to keep up a 'Q' car appearance in the rear view mirror of the pursued. The Tiger is fitted with a 302 cu in engine and comes with a fully restored hard top in matching paint, Dunlop safety wheels but otherwise to a standard specification. Featured in Practical Classics Spring 2012. The car has been used regularly over the years for local shows, longer trips and continental touring and has proved to be fast, fun and reliable transport. MOT and TAX exempt but the car has an MOT with no advisories to 22/5/2020. There is even a model of the car available!
Audi Quattros are referred to among owners and enthusiasts by their
engine codes, the earliest 2,144 cc 10v being the "WR" engine, the 2,226 cc 10v being
the "MB" engine, and the later 20v being the "RR" engine. This car is an MB Quattro that was first registered 1st January 1989. Production of the Quattro totaled 11,452 units over the period from 1980–1991and
through this 11 year production span there
were no major changes in the visual design of the car. For the 1983 the
dashboard did away with an instrument cluster and now fitted with a
green digital LCD cluster.
This was later changed in 1988 to an orange LCD electronic instrument
cluster. The interior was redesigned in 1984, and featured a whole new
dashboard layout, new steering wheel design, and new centre console
design, the switches around the
instrument panel were also redesigned at this time. This car is finished
in the very distinctive colour of Tornado Red and comes with the
optional Black Leather interior. The car remains in very good condition
having benefited from a body respray some time ago and a recent engine
rebuild and mechanical overhaul completed by marque specialist The Quattro Workshop. There is a large history file full of
old invoices and MOT's and even the original sales invoice from 1989
when the car cost £32,328.50. Since then the car has had 6 owners and
covered only 139,000 miles.
1972 MORRIS MINOR 1000 8CWT VAN. 1275cc MG ENGINE.
This really nice Morris Minor 1000 van was first registered 1st March 1972 and is the rarer 8cwt model and it must be one of the last Minor vans registered. The lovely paintwork in Trafalgar Blue looks great with the cream wheels fitted with the chrome trim rings. Inside the van the seats have been replaced with some comfy bucket seats and it has been fitted with harness style seat belts. The original seats come with the van should the new owner wish to fit them. Under the bonnet is a bit special with a freshly rebuilt 1275cc engine and rebuilt gearbox from an MG midget with twin carbs and a rather throaty side exit exhaust. The engine has only just been fitted so a running in period would be advised. It certainly sounds and goes a lot better than standard and is a hoot to drive. There is an original workshop manual, drivers handbook and sales brochure along with some receipts and MOT's in the file. It has also been fitted with an 81 sound machine maker and a very loud Klaxon horn that makes people get out of the way! The van is now 47 years old so is Road Tax and MOT exempt.
This 1969 MGC Roadster finished in Mineral Blue is a UK car and was first registered 4th December 1969 so is this month 50 years old. The mileage shows only 73,000 miles which is believed to be correct. It is road tax and MOT exempt. The car was kept off road for several years and has recently (this year) had an extensive restoration by a leading MG Specialist. The engine has been rebuilt by the famous engineering company Oselli and the car has been little used since then. It runs and drives beautifully with the 3 litre 6 cylinder engine sounding fantastic and giving plenty of performance. The knock on wire wheels have been powder coated in a chrome effect finish. The interior is very tidy with the seats finished in black leather with white piping. The convertible top is in excellent condition and there is a full tonneau cover with the car. These cars are now now rare and hard to find in this condition and make a good investment.
you get a car that is a bit special! This VW is one of those special
cars. It is just amazing. You will need to come and see the car to get
the full picture as it is hard to describe just how nice the car is.
This VW Beetle 1200 started of life in 1959 when it was purchased by a
British serviceman serving in Germany. It was from new a RHD car. He
returned to the UK in 1961 with the car and it was registered8th July
1961. It was then part exchanged for a newer model at
the local VW dealership where a young apprentice mechanic took a shine
to it and purchased it for a modest sum. That young apprentice still
owns the car today some 58 years later. He went on to be a top VW
engineer and mechanic traveling regularly to Wolfsburg to do all the VW
courses. In 1979 the bug was put away in a dry garage and carefully
stored until he retired from work and had the time and expertise to
restore the bug to its original beauty. The car was completely
dismantled and shot blasted back to bare metal. No new panels where
needed and the car retains all original panels, original glass and even
original headlamps. The car has had an excellent repaint in the original
colour of Mango Green. The interior has also had a full and meticulous
restoration including the headling that had to be sourced from the USA.
Whilst the body was off the floorpan and running gear was serviced as
required, the engine was dismantled and refreshed, all tinware was stove
enamelled in the original black. All brakes have been overhauled with a
new master cylinder, wheel cylinders, brake pipes, hoses, brake drums,
wheel bearings, brake
1967 WOLSELEY HORNET Mk3. PORCELAIN GREEN. SUPERB CONDITION. 67,000 MILES ONLY. £8995.
This great Wolseley Hornet Mk3 was first registered in Sussex 18th May 1967. The car has remained in the Sussex area for most of its life and has traveled only 67,000 miles in those 52 years. The last 2 owners keeping the car for 32 years. The car looks fantastic finished in the original Porcelain green with the dark green roof. It has a contrasting Green leather interior in good condition with nice trim and carpets. The car has had a rebuild (with pictures) and the engine was rebuilt just 5000 miles ago. The history file has 13 old MOT's, the old green log book and lots of invoices going back to 1967 when the car was new. These all show the considerable love and care the car has received throughout its life and proves that the mileage is correct. recent items include: new tyres, brake drums, brake shoes, battery, timing chain, water pump, fan belt, rocker gasket, points, leads, wheel bearings, brake cylinders front and rear, brake pipes, oil seals, oil filter, and new shock absorbers. Being 52 years old it is both Road Tax and MOT exempt. The 998cc engine and 4 speed gearbox are in excellent order and propel the car down the road with gusto! The ride is very good on the dry Mini suspension and all the car needs is a new owner.
What a great looking car this is. The pillarless coupe design works so well and is very reminiscent of the Plymouth Barracuda. This Sunbeam Rapier was first registered 8th June 1972. The car is now 48 years old and qualifies for both road tax and MOT exemption. The colour is the original Caribbean Blue and it has just been treated to a respray after the sills had been replaced. The car has had only 3 owners in 48 years (18 + 18 + 12) and remarkably has covered only 52,000 miles in that time. The MOT history shows that in the last 14 years the car has covered only 6000 miles and passed every time without any advisories. The 1725cc straight 4 engine with 4 speed + overdrive gearbox is very nice and gets the car down the road very well. There is a large file with lots of old mag articles and original adverts etc and a owners handbook and 2 workshop manuals. The boot area is really good and the engine bay is very tidy. The body and underside are both very solid and original.
This great little Triumph Toledo 1.3 4 door saloon was first registered 9th March 1976 so is nearly 44 years old and has averaged less than 2500 miles per year during that time. 2 owners kept the car for over 32 years. It is now road tax and MOT exempt. In the history file there are lots of old invoices going back to 1987, magazine articles and 36 old MOT's (out of a possible 40) that proves the car cars mileage and that it has been regularly but sparingly used with only 23,000 in the last 20 years. Although it is MOT exempt it does have a current MOT and the only advisory has been rectified. There is also a heritage certificate with all the details of the car when new. The car is finished in Carmine Red and is in excellent condition all around. The interior is also in good original order with reclining seats, inertia seat belts both front and rear, Dolomite style door cappings and a MW/LW radio. This car has had a lot of money spent on its improvement and upkeep and in recent times has had the following: New alternator, wiper motor, solenoid, coil leads, dizzy cap, points, condensor, carb, radiator, water pump, fuel pump, petrol tank, electronic ignition, brake calipers and pads, thermostat, clutch slave cylinder, clutch master, rear brake hose, brake shoes, road springs, battery, carpets, grill, unleaded fuel cat, radiator hoses, full stainless steel exhaust and more! It has 5 new tyres and a recent service. The underside is in excellent order as is the boot area and the engine bay. The 1296cc engine is nice and smooth and matched to the 4 speed gearbox propels the car along nicely.
This is a 1968 MGB GT GHD4 car finished in Mineral Blue that was first registered 9th August 1968. It has wire wheels, a black leather with white piping interior, overdrive gearbox and a Moto Lita steering wheel. A very desirable specification. There is a good history file that shows us that by the late 90's the car was now over 30 years old and showing its age. The then new owner Bob Burgess decided to have a full re-build and entrusted the job to classic car specialist Classic Encounter in Surrey. It was decided to re-shell the car, fit 4 new wings and any other panel work needed before a full bare metal painting in Mineral Blue. A new engine with unleaded heads was purchased from the MG owners club and fitted along with a reconditioned gearbox with overdrive and a new clutch. It also received electronic ignition, stainless steel exhaust, brake servo, brake pipes, wiring loom and a new leather interior. Over £10,000 was spent and that was then! The process recorded in a series of photographs in the file. Since that time (23 years) the car has covered only 8,000 miles in which time it has also had a 12v battery conversion, front shock absorbers, a Kenlow electronic fan, starter motor, trunnions, air horns, oil cooler and a clifford alarm (untested) fitted. The car has good oil pressure, new tyres, looks fantastic and drives very well.
1964 SUNBEAM ALPINE S4. 1600cc. OVERDRIVE. JUST HAD FULL RESTORATION. WEDGEWOOD BLUE. £17,995.
This is a 1964 Sunbeam Alpine Series 4 with a 1600cc engine and 4 speed/overdrive gearbox. It is finished in Wedgewood Blue which I think is the best colour for these cars. 1st registered KMG 856B on 24th August 1964 it is now 56 years old and both Tax and MOT exempt. The mileage is showing 12,900 so we must assume that it is 112,000. An old mot from 1993 in the history file shows the car at 12,204 miles so it has only done 700 miles in the last 27 years. Nothing known before 1993 when the car was purchased by a Kenneth Hicks of Pulborough. It was the cars next owner Tim Collins who started to update the Alpine. By 2010 the car was still in Sussex and now owned by a chap in Bexhill who stripped the car down to restore it but unfortunately passed away before he could complete the job. His friends at the local car club took up the challenge for him and completed the full restoration in 2019 spending over £9000 on parts alone. Their labour was free and the final bill would have been well over £20,000. The car had been stripped to bare metal, restored with new panels and repainted. Engine was rebuilt and gearbox overhauled. All new brakes, clutch, suspension, polybushed, brake/fuel lines, radiator, chrome fittings, rubber seals, new hood, stainless exhaust, 4 branch manifold, new Weber carb, stainless bumpers and new wiring loom. There is more in the history file. The car has been waxoiled for future protection and is ready to go for the summer season.


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Atlas Aluminium datasheet 5052 rev Oct 2013 - Atlas Steels
Recommended minimum bending radius for sheet of thickness given, at 90°to the rolling direction. Standard Specification for High Magnesium Aluminum-Alloy Sheet Service and Similar Environments Microsoft Word - Atlas Aluminium datasheet 5052 rev Oct 2013.doc Author: moorep Created Date:Send Inquiry
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94 points from Robert Parker: "The light gold-colored 2006 Chardonnay Cahill Lane exhibits a steely, stony nose intermixed with peach, pear, and brioche notes. It is medium to full-bodied, has terrific up-front fruit, decent acidity, and a plump, medium to full-bodied mouthfeel. This is the most concentrated of the first three Chardonnays, but seems to be on a faster evolutionary track than any of the 2005s. A terrific source of sensational Pinot Noirs and Chardonnays made very much in the style of the wines proprietor Stephen Hansel loves the most, white and red Burgundies, this 80-acre estate continues to build on a brilliant track record of high quality wines offered to consumers at sensationally fair prices." (12/07) 90 points Stephen Tanzer's International Wine Cellar: "Light, bright gold. Spicy citrus and pear on the nose, with suave minerality adding focus. Riper melon and tropical fruits on the palate. Silky in texture, with medium weight and a hint of white pepper that carries into the finish. The most fruity of this set of chardonnays, offering a very attractive blend of sweetness and energy." (May/June 08)
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lifeless hand, he rivetted his eyes on the pallid features,
now settling into a placid smile, as frequently happens to
the physiognomy where death has resulted from a gunshot
Raby," said the Creole, and his voice sounded supernaturally
" the dead are dead, and the living
must not be lost rise up and away !
" "
No," replied Raby, I will not stir. Come what
may, this is my proper place, and till the tomb bars me
from him, thus will I sit at his head as his chief mourner."
" This is mere madness," replied the Creole, in a
sharper tone, and with a gesture of
impatience. The
cry of murder will rise hotly against you blood will
demand blood and your own parent even will not be
able to save you. One son is gone, and if another must
follow, at least spare us trie spectacle of an ignominious
For your father's
sake for the sake of one still
Raby groaned at this new blow, and dropped his brother's
hand. Mechanically he rose up and turned his eyes
towards Hawksley, and scalding tears for the first time
gushed from the parched lids, as he thought of the young,
fond, joyous heart he was doomed to break. Something
he tried to say, but the sound died upon his lips ; his head
drooped, his arms dropped powerless by his side, and he
assumed at once the despairing attitude and expression of
a wretch who had just stepped irretrievably over the threshold
of that tremendous portal, in Dante, beyond which
hope has not even a name.
" "
Hark a shot ! exclaimed the Creole, in an under
tone "
; the keeper is going his rounds if you remain
here you are lost ! and he seized the arm of Raby, and
partly by force led him from the fatal spot. The condition
of the latter was truly pitiable, and, to estimate it, the reader
must consider not merely the harrowing circumstances of
the time, but the peculiar morbid sensibility and constitutional
nervous temperament of the individual, which com-
bined to deprive him of all ordinary firmness. In utter
prostration of mind and body, with a complete paralysis of
purpose, and the absolute apathy of despair, he implicitly
u 3


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Tuberculosis (TB) is the leading cause of death among infectious diseases worldwide. Among the estimated cases of drug-resistant TB, approximately 60% occur in the BRICS countries (Brazil, Russia, India, China and South Africa). Among Brazilian states, primary and acquired multidrug-resistant TB (MDR-TB) rates were the highest in Rio Grande do Sul (RS). This study aimed to perform molecular characterisation of MDR-TB in the State of RS, a high-burden Brazilian state. We performed molecular characterisation of MDR-TB cases in RS, defined by drug susceptibility testing, using 131 Mycobacterium tuberculosis (M.tb) DNA samples from the Central Laboratory. We carried out MIRU-VNTR 24loci, spoligotyping, sequencing of the katG, inhA and rpoB genes and RDRio sublineage identification. The most frequent families found were LAM (65.6%) and Haarlem (22.1%). RDRio deletion was observed in 42 (32%) of the M.tb isolates. Among MDR-TB cases, eight (6.1%) did not present mutations in the studied genes. In 116 (88.5%) M.tb isolates, we found mutations associated with rifampicin (RIF) resistance in rpoB gene, and in 112 isolates (85.5%), we observed mutations related to isoniazid resistance in katG and inhA genes. An insertion of 12 nucleotides (CCAGAACAACCC) at the 516 codon in the rpoB gene, possibly responsible for a decreased interaction of RIF and RNA polymerase, was found in 19/131 of the isolates, belonging mostly to LAM and Haarlem families. These results enable a better understanding of the dynamics of transmission and evolution of MDR-TB in the region.
Gluten is only partially digested by intestinal enzymes and can generate peptides that can alter intestinal permeability, facilitating bacterial translocation, thus affecting the immune system. Few studies addressed the role of diet with gluten in the development of colitis. Therefore, we investigate the effects of wheat gluten-containing diet on the evolution of sodium dextran sulphate (DSS)-induced colitis. Mice were fed a standard diet without (colitis group) or with 4·5 % wheat gluten (colitis + gluten) for 15 d and received DSS solution (1·5 %, w/v) instead of water during the last 7 d. Compared with the colitis group, colitis + gluten mice presented a worse clinical score, a larger extension of colonic injury area, and increased mucosal inflammation. Both intestinal permeability and bacterial translocation were increased, propitiating bacteria migration for peripheral organs. The mechanism by which diet with gluten exacerbates colitis appears to be related to changes in protein production and organisation in adhesion junctions and desmosomes. The protein α-E-catenin was especially reduced in mice fed gluten, which compromised the localisation of E-cadherin and β-catenin proteins, weakening the structure of desmosomes. The epithelial damage caused by gluten included shortening of microvilli, a high number of digestive vacuoles, and changes in the endosome/lysosome system. In conclusion, our results show that wheat gluten-containing diet exacerbates the mucosal damage caused by colitis, reducing intestinal barrier function and increasing bacterial translocation. These effects are related to the induction of weakness and disorganisation of adhesion junctions and desmosomes as well as shortening of microvilli and modification of the endocytic vesicle route.
Diarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 32.5% for EAEC, 6.3% for ETEC, 4.4% for EIEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies.
The aim of this study was to evaluate the effect of dietary lysine on performance, protein deposition and respiratory chain gene expression in male broilers. A total of 252 Cobb 500 broilers were distributed, in a completely randomized design, into four treatments with seven replicates of nine birds per experimental unit. Experimental treatments consisted of diets based on corn and soybean meal, with four levels of digestible lysine: 1.016%, 1.099%, 1.182% and 1.265%. The increase in the level of digestible lysine in the diet provided higher weight gains, feed efficiency and body protein deposition. Birds fed the lowest level of dietary lysine (1.016%) showed a lower expression of genes such as NADH dehydrogenase subunit I (ND1), cytochrome b (CYTB) and cytochrome c oxidase subunits I (COX I), II (COX II) and III (COX III), displaying the worst performance and body protein deposition. This demonstrates the relationship existing between the expression of the evaluated genes and the performance responses. In conclusion, results indicate that broilers fed diets with higher levels of digestible lysine have increased messenger RNA expression of some genes coded in the mitochondrial electron transport chain (ND1, CYTB, COX I, COX II and COX III). It may be stated that diets with proper levels of digestible lysine, within the 'ideal protein' concept, promote the expression of genes, which increases the mitochondrial energy, thereby fostering body protein deposition and the performance of broilers in the starter phase.
During radiation therapy, unwanted scatter to healthy tissues outside the target field may occur. Children and adolescents are more sensitive to radiation injury, and the thyroid gland is particularly susceptible to these effects.
To assess acute changes in thyroid function and volume in children and adolescents undergoing radiotherapy for a variety of non-thyroid cancers.
Thirty-one children and adolescents underwent radiation therapy of various body areas in which the thyroid was not included. Thyroid-stimulating hormone (TSH), thyroxine (T4), free thyroxine (fT4), triiodothyronine (T3), anti-thyroperoxidase antibodies and thyroglobulin were measured before, on the last day and at 1 and 3 months after the end of radiotherapy. Ultrasound scans were taken and 6- and 24-hour 131I uptake was measured before and after treatment. The scattered dose to the thyroid region was estimated with a treatment planning system or measured with thermoluminescent dosimeters.
The median radiation dose scattered to the thyroid was 296·6 cGy (IQR 16·7–1,709·0). Levels of TSH (p = 0·575), T4 (p = 0·950), fT4 (p = 0·510), T3 (p = 0·842), thyroglobulin (p = 0·620) and anti-thyroid peroxidase antibodies (p = 0·546) were statistically similar at all four time points. There were no differences between pre- and post-radiotherapy thyroid volume and 131I uptake (p = 0·692 and 0·92, respectively).
More sensitive methods may be required to ascertain whether acute injury to the follicular epithelium occurs with lower radiation doses scattered to the thyroid.
The objective of the current study was to evaluate the utilization of calcium (Ca) and phosphorus (P) in growing sheep consuming increasing amounts of dicalcium phosphate. Eighteen growing sheep, aged 8 months, were fed a basal diet supplemented with 0, 12·5 and 25 g of dicalcium phosphate/day. During the experiment, animals were injected intravenously with 7·4 MBq of 45Ca and 32P and samples of plasma, faeces and urine were subsequently taken daily for 1 week after injection. Rumen fluid was sampled on days 4–7 after injection. Specific radioactivity in plasma and in faeces were used to determine true absorption of Ca and P, whereas plasmatic and ruminal specific radio-activities were used to determine endogenous P flow into the rumen and turnover time of rumen P. Increasing dicalcium phosphate intake led to linear increases in faecal excretion of endogenous Ca and P (P<0·05), suggesting that surpluses of ingested Ca and P were voided through secretion to the gut. True absorption coefficients for 0, 12·5 and 25 g of dicalcium phosphate ingested daily were 0·54, 0·41 and 0·38 for Ca, and 0·66, 0·62 and 0·64 for P, respectively. Flows of endogenous P into the rumen increased linearly and ruminal turnover time of P decreased linearly (P<0·01) as P intake was increased. Concentrations of Ca and P in bone were not affected by the increased amounts of these minerals ingested (P<0·05). In conclusion, increasing ingestion of dicalcium phosphate increases faecal excretion of Ca and P, thus decreasing the efficiency of utilization of both minerals. Moreover, increasing levels of dietary P increased endogenous P excretion, contributing to the amount of P disposed of in the environment.
The ability to control the deposition of mouse embryonic stem cells (mESCs),
and mESCs encapsulated in 200-μm diameter alginate microbeads, into
customized patterns has recently been achieved using laser direct-write
(LDW). Gelatin-based LDW was utilized to target and reproducibly deposit
groups of cells directly onto receiving substrate surfaces. Live/dead
staining for cell viability and immunocytochemistry for the pluripotency
marker, Oct-4, indicated that transferred mESCs were viable following
transfer, and maintained an important embryonic stem cell marker,
respectively. LDW was further used to print mESCs encapsulated in hydrogel
microbeads into customized patterns on a single-bead basis. Recent efforts
have also achieved patterns of discrete co-cultures of mESCs and breast
cancer cells in separate hydrogel microbeads. Altogether, we demonstrated
the feasibility of LDW to print patterns of mESCs and mESC-microbeads for
the biomimetic assembly of engineered cellular constructs and tissue
Chagas' disease is a debilitating but comparatively neglected illness that affects about 15 million people. There is an urgent need to develop new, more effective, and less-toxic compounds. In this study, we assessed the in vitro anti-trypanosomal activity of the sesquiterpene elatol from the Brazilian red seaweed Laurencia dendroidea. We used electron microscopy to evaluate the effect of elatol on the morphology and ultrastructure of the parasite. Elatol showed a dose-dependent effect against the epimastigote, trypomastigote, and amastigote forms, with IC50 values of 45·4, 1·38, and 1·01 μm, respectively. Observation of treated intracellular amastigotes by light microscopy demonstrated a total elimination of the infection at a dose of 3·0 μm. In addition, the compound did not affect the red blood cells, and the CC50 value for LLCMK2 cells was 27·0 μm. Transmission and scanning electron micrographs showed aberrant-shaped cells and breaks in the plasma membrane, prominent swollen mitochondria, and extensive formation of cytoplasmic vacuoles in all the forms. This is the first report of the anti-trypanosomal effect of the sesquiterpene elatol.
The objective of the current study was to apply the Vitti–Dias model to investigate phosphorus (P) metabolism in growing pigs fed a diet supplemented with microbial phytase. The basal diet contained maize, defatted rice bran, vegetable oil, soybean meal, limestone, salt and a vitamin and mineral mix. There was no inorganic P in the diet and phytase was added at levels of 253, 759, 1265 and 1748 phytase units (PU)/kg of feed. The compartmental model included four pools of P: (1) gut lumen, (2) plasma, (3) bone and (4) soft tissue. A single dose of 32P was administered, and specific radioactivity was measured in plasma, faeces, bone and soft tissue (muscle, heart, liver and kidney) at different times post-dosing for calculation of P flows between pools. Total P absorbed showed a negative relationship with total P excreted in faeces and was strongly correlated with bone P retention, suggesting that absorbed P was channelled to bone to address its physiological growth. Average efficiency of metabolic utilization of absorbed P was estimated to be 0·94, with 0·52 g/g of total net P balance being accreted in bone and the rest in soft tissue (including muscle and some vital organs). The Vitti–Dias model provided suitable representation of P interchange between compartments (in particular, flows between gut and plasma and partitioning of available P between bone and soft tissue), resulting in estimates of P flows comparable with values calculated from balance data.
We consider the two-dimensional version of a drainage network model introduced in Gangopadhyay, Roy and Sarkar (2004), and show that the appropriately rescaled family of its paths converges in distribution to the Brownian web. We do so by verifying the convergence criteria proposed in Fontes, Isopi, Newman and Ravishankar (2002).
Inflammation is a stereotypical physiological response to infections and tissue injury; it initiates pathogen killing as well as tissue repair processes and helps to restore homeostasis at infected or damaged sites. Acute inflammatory reactions are usually self-limiting and resolve rapidly, due to the involvement of negative feedback mechanisms. Thus, regulated inflammatory responses are essential to remain healthy and maintain homeostasis. However, inflammatory responses that fail to regulate themselves can become chronic and contribute to the perpetuation and progression of disease. Characteristics typical of chronic inflammatory responses underlying the pathophysiology of several disorders include loss of barrier function, responsiveness to a normally benign stimulus, infiltration of inflammatory cells into compartments where they are not normally found in such high numbers, and overproduction of oxidants, cytokines, chemokines, eicosanoids and matrix metalloproteinases. The levels of these mediators amplify the inflammatory response, are destructive and contribute to the clinical symptoms. Various dietary components including long chain ω-3 fatty acids, antioxidant vitamins, plant flavonoids, prebiotics and probiotics have the potential to modulate predisposition to chronic inflammatory conditions and may have a role in their therapy. These components act through a variety of mechanisms including decreasing inflammatory mediator production through effects on cell signaling and gene expression (ω-3 fatty acids, vitamin E, plant flavonoids), reducing the production of damaging oxidants (vitamin E and other antioxidants), and promoting gut barrier function and anti-inflammatory responses (prebiotics and probiotics). However, in general really strong evidence of benefit to human health through anti-inflammatory actions is lacking for most of these dietary components. Thus, further studies addressing efficacy in humans linked to studies providing greater understanding of the mechanisms of action involved are required.
The objective of the present study was to examine the effect of the level of phosphorus (P) intake on ruminal P kinetics in sheep. Twelve Santa Inês male sheep (average body weight 36 kg) were fed a basal diet consisting of roughage (coast cross hay), concentrate mixture (cassava meal, soya bean meal and urea) and a mineral premix. The treatments consisted of the basal diet supplemented with 0, 1·5, 3 or 4·5 g/kg dry matter (DM) of mono-ammonium phosphate to provide increasing P levels representing treatments T0, T1, T2 and T3, respectively. The P content of experimental diets was 1·5; 2·0; 2·5 and 3·0 g P/kg DM, and considered highly deficient, deficient, adequate and in excess, respectively, compared with standard recommended allowances. Animals were injected with 32P and thereafter samples of blood were collected over 7 days, while samples of rumen fluid and saliva were collected 4 and 6 days after injection. Phosphorus intake affected P concentration in ruminal fluid, whereas P concentration in saliva was not affected. The values for P turnover time in the rumen were 1·42, 1·23, 1·18 and 1·04 days, whereas values of endogenous P entry into the rumen were 1·05, 1·37, 1·53 and 1·91 g/day for T0, T1, T2 and T3, respectively, both affected by P intake. The specific activity (SA) of P in saliva, rumen and plasma were also all affected by P intake. The relationship between saliva and rumen SA emphasizes that most endogenous P in the rumen came from saliva. The possibility of an extra P source besides saliva contributing to endogenous P in the rumen is discussed. It is concluded from the results that, regardless of P intake, the flow of endogenous P into the rumen contributes to ensure a minimum supply of this essential element, which may be important in matching the requirements of the rumen microbes.
In Northeast of Portugal sheep is reared under extensive systems. These systems frequently involve expressive body composition changes due to the storing and mobilization of body reserves, mainly fat. Body condition score (BCS) is the most common way to assess these reserves and the nutritional status of ewes. However due to the subjective nature of BCS, their quality has been questioned and other alternatives has been studied. For cattle there are some studies that use the real time ultrasonography (RTU) to evaluate the BCS (Schwager-Suter et al., 2000; Broring et al., 2003), but this approach was not tested in ewes. Therefore the aim of the present study was to establish a relationship between the BCS and ultrasound subcutaneous fat (SF) and Longissimus thoracis et lumborum muscle (LM) measurements.
This study investigated the effect of environmental variables such as soil class, soil water availability, topography and slope on spatial distribution patterns of tree species in a Brazilian Seasonal Semideciduous Forest area. Floristic and structural data for a tree community were obtained by sampling 100 plots 10 × 10 m in which every tree with trunk diameter (dbh) ⩾ 4.77 cm at 130 cm above ground level was sampled. The area under study showed a marked soil gradient, directly associated with the topography: flat hilltops with Al3+-rich Dystric Latosols give way to steep colluvial slopes with shallower and more Dystric Cambic Latosols without Al3+, changing over, at the bottom of the hollows, to Epieutrophic Cambisols richer in nutrients. The floristic-sociological parameters analysed for the soil habitats did not differ statistically from each other. The diversity and equability indices were 3.6 and 0.84, 3.48 and 0.85, 3.49 and 0.84 for the Dystric Latosol, Dystric Cambic Latosol and Epieutrophic Cambisol, respectively. The soil variables (related to the fertility and texture) and the soil water regime (drainage) were probably the principal factors determining the spatial distribution patterns of tree species in the forest.
The purpose of the current work was to study phosphorus (P) metabolism in growing sheep supplemented with different levels of dicalcium phosphate using an extant mathematical model. Twelve male non-castrated Santa Inês sheep, weighing 23 (±2·2) kg, received a basal diet unsupplemented or supplemented with dicalcium phosphate to provide 1·5, 3·0, 4·5 g of P/animal per day (treatments T1 to T4, respectively). After 3 weeks adaptation, 7·4 MBq of 32P was injected into the jugular vein of each animal. Samples of blood, faeces and urine were collected every day during a 7-day period and thereafter the animals were sacrificed and samples from liver, kidney, heart, muscle and bone were collected for specific activity and inorganic P determinations. The flows between gut and plasma were similar for each treatment except for T1, which showed the lowest values for both flows (P<0·05). The amount of P accreted to soft tissue (F42) was different among treatments, however net tissue retention was similar for all treatments. Total P retained was highest for T4 and lowest as well as negative for T1 and T2. Phosphorus accreted to bone (F32) was different among treatments and contributed to the different net bone retentions. The highest value of F32 was reached by animals on T4, whilst the lowest values were found for animals on T1. Despite having the highest value of F32, it should be noted that animals on T4 excreted the most P in faeces. Considering concerns about environmental P pollution, it is important to be aware that the treatment which provided the highest value for net bone P retention and for F42 also led to the highest value of P excreted in faeces. Therefore, the current study suggests that T3 provided the best P level for this category of animal since P accreted to bone and tissue indicated that P absorption was adequate to attend to P requirements.
Cereal grains are the most common sources of readily available energy for dairy cows. Several in vitro and in situ studies have measured starch degradability of cereal grains. However, most of these studies compared few samples and grain species and used only one method. Compared with protein, much less information is available on this topic. Such information would allow a more efficient use of energy and protein in dairy cow diets. The objective of this study was to determine starch degradation of 32 samples of 7 cereal grains using in vitro incubation with rumen fluid and a new method based on a boiling extraction with an enzymatic (thermo stable α-amylase) solution.
In sheep production the ability of the animal to retain and mobilize body fat reserves is of considerable importance in determining the sheep productivity or even its survival. The most common way to predict body fat reserves is the body condition score. On the other hand, it is accepted that breeds have a different fat distribution within the body (Taylor et al., 1989). In general, ewes breed for milk production tend to deposit more fat in internal depots and those breed for meat production deposit more fat in the carcass depot (Frutos et al., 1997). This kind of fat distribution can explain the use of an objective and more precise way to predict the internal fat in ewes of the Churra breed, in which the internal fat depots plays an important role. Thus, it is reasonable hypothesize that the internal fat depots are related with muscle and subcutaneous fat measurements. As a result, the study herein reported was undertaken to achieve the relationship between real time ultrasound measurements obtain over thoracic, lumbar and sternum regions to predict the internal fat depots of Churra da Terra Quente (CTQ) milk breed.
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Fall is a busy time of year for all of us. With school, sports, holidays and family gatherings, hom
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Tunde Ednut Owes Me Thanks For Helping His Career – Bobrisky
Nigerian self-acclaimed transgender, Okuneye Idris, who is popularly known as Bobrisky said he came in peace, but also ready for anyone that wants 'pieces'.
The crossdresser, stated today, on social media that Tunde Ednut should be thanking him for making his career grow as an Instagram influencer through his constant push.
Naija News recalls that Bobrisky yesterday took to social media to reveal the only condition that will make him date a married man.
According to Bobrisky, aside from paying his bills, such a man must be willing to stay low-key and love must be involved.
"I'll love to date a married man for one reason. Both of us need to have a level of understanding that the relationship would be low-key between just two parties: myself and the person involved," he said.
"We don't want a loud thing. Even my best friend won't know about it. If both of us have this agreement, I can date a married man in as much as he's paying my bills and we love ourselves."


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Price: $31.00
Quantity: 1 available
Book Condition: Good
192 pages. Boards have no wear, rubbing or soiling. Spine has moderate lean. Edges of dust jacket have moderate wear and moderate chips and/or tears. Dust jacket is unclipped. Dust jacket is protected in clear, plastic sleeve. Pages are reasonably tanned. Previous owner's name in ink. Quantity Available: 1. Category: Fiction; Crime; Inventory No: 18040312. Hardcover
Title: Death By Marriage
Edition: First Edition
Publisher: London, John Gifford: 1959
ISBN Number: No ISBN
Binding: Hardcover
Book Condition: Good
Jacket Condition: Good
kg: 0.29 kg
Seller ID: 18040312
Keywords: BZDB5 Fiction; Death By Marriage Hardcover,