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Part IA: Family Interview: Research your family medical history. Go back as far as your maternal and paternal grandparents. Make sure that you include aunts, uncles, cousins, brothers, sisters, parents and children. Interview family members and ask the following questions:
Total – 50 Points
Tell us about your assignment and we will find the best writer for your paper.Write My Essay For Me
Common Chronic Illnesses: Breast Cancer, Ovarian Cancer, Heart Disease, Stroke, Colon Cancer, Diabetes
1. What diseases or medical conditions do your family members have?
2. Does anyone in your family have one of the common chronic illnesses listed above? Has anyone in your family died from one of the common chronic illnesses listed above?
3. List and discuss the illness or conditions that people in your family has died from. Your answer should be detailed.
4. What effect did these diseases or conditions have on your family member and family? Your answer should be detailed.
5. What lessons did each family member learn about maintaining health? Ask each family member that you interview this question so that you can compile a list of responses to include in your reflection paper. Their answers can include lessons about healthy eating habits, taking herbal medications, exercise habits, prayer, meditation etc. Write down all of the information that you learn.
Note: Please submit the answers to these questions in a Word document.
Part IB: Create a Family Health Portrait: After interviewing your family members and gathering the necessary information, create a family health portrait. The link to the electronic Family Health Portrait document is located in Blackboard.
Total – 50 Points
Family Health Portrait Document: Please download this document in Blackboard. This document is located in the "Family Health History Project" folder.
Note: In order to get full credit for this assignment, please make sure that you complete and submit this document.
Family Portrait Document Submission: You can scan and upload this document in the submission folder below or you can take pictures of the completed documented with your smart phones and submit the pictures. If you choose to take pictures of the completed document, please make sure that it is clear picture.
Part II – Family Health History Project- Reflection Paper
Your reflection paper should be 2-3 pages long.
The total amount of points you can earn for this paper is 100 points.
APA Style Requirement: Your paper should be written in APA style. APA style includes a typed paper that is double spaced, on standardized paper (8.5 x 11) with 1" margins on all sides and written in 12 point Times New Roman Font. Details about citing various sources will be given in detail separately.
Reference: ( http://owl.english.purdue.edu/ ).
Note: You are not required to use information from outside sources for this project. However, you are required to cite any information that you retrieve from other sources in APA style. For example, if you chose to define a medical condition, please make sure that you cite the source in the body of your paper and in the reference list. Failure to cite any information that you get from other sources is plagiarism.
Introduction: (30 Points)
In the introduction of your paper, briefly discuss your family background. Your introduction should include the following:
1. Grandparents: Where are your mother's parents from? Where are your father's parents from? What was their childhood like?
1. Parents: Where is your mom from? Where is your father from? What was their childhood like?
1. What did your grandparents and parents learn about maintaining health?
1. How many aunts and uncles do you have on each side of the family (include your mom's siblings and your dad's siblings).
Body of Paper: (50 Points)
The body of your paper should provide detailed information about your family's medical history.
The body of your paper should include the following:
1. Current Family Medical Conditions: What medical illnesses or conditions do your family members have? Discuss the diseases and conditions that you were aware of prior to this project. Discuss the diseases and conditions that you learned about during your family interview.
1. Disease Burden Impact: How has the disease or condition impacted the life of your individual family member(s)? How has the disease or condition impacted your family? If there are hereditary health issues, how can these health issues impact your life? Has anyone died from any of the diseases or conditions?
1. Best Practices- Disease Prevention: What steps can you take to prevent developing this illness? Discuss at least 3 healthy lifestyle habits or behaviors.
Conclusion: (20 Points)
The conclusion of your paper should include the following:
1. Summarize your findings.
1. What did you learn after completing this project?
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Speak your truth
Updated: Jan 23, 2021
I received an email from Sheryl. She wrote:
A year ago, I invited a few friends to join me in a virtual Biodanza session. I did an FB live post doing a sacral dance prior to the session.
A friend blatantly said, "law-ay, way angay" (scandalous, unbecoming).
It was a trigger, so I retreated to the comfort of pain and depression and decided that I am done with sharing my meditation practice. I decided to become the same bullied little girl and dance in the confines of my home but I continued with deepening the practice of meditative dance along with workshops by Fr. Loreto Jaque.
I never really regained the confidence in sharing the practice with people I love so I posted carefully curated still shots of my dance practice instead.
"Sheryl, speak your truth". Like a lightning bolt, Doc Sara Racal's words fired the dying engines in my heart.
With a video on meditative dance, I spoke my truth again and with self-love. A few friends asked and now some of them are slowly practicing meditative dance.
We are sensual beings, sacral dance is the language of the untethered primal energy of our bodies. It expresses nothing but the passion and openness for life, free of self-judgment. Dance, is my truth, spoken.
Gratitude to OHC and for Doc Sara for helping me manifest my "being".
I love how Doc Sarah's words ignited Sheryl's courage to stand in her truth.
That's why we need mentors- to nudge us and remind us of our truth and greatness.
Watch this class and let me know what it brings out in you:
I would love to hear from you!
And, may you always stand in your truth.
Stay brave,

subset: commoncrawl


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Nexcess Released Upgraded Magento Hosting and New Server ClustersApril 25, 2019
Managed to host provider Nexcess is a Southfield, Michigan based company founded in 2000 with wholly-owned data centers located in Dearborn, Michigan and Southfield, Michigan. The company is very popular for providing shared and dedicated Magento, WordPress and ExpressionEngine hosting, has now come up with a host of improvements to its Magento hosting packages specifically for the company's enterprise solutions. Nexcess has extended its range of high end cluster options for Magento hosting with the new MCE-SIP-400 package that offers best possible response times for eCommerce stores to provide reliable and powerful hosting environment.
The new cluster package of Nexcess includes six Dell Power Edge R420 servers with 2 Eight Core E5-2450 Sandy Bridge Processors, 24 GB of RAM, and a pair of 73 GB 10K SAS drives behind a Dell Power Edge 1950 load balancer. With the new updated cluster option, there are lot of benefits including increase in bandwidth provisions, increase in CDN bandwidth. In addition to that, Data usage has been increased to 10,000 GB with 5,000 GB of content delivery network bandwidth included. According to Nexcess, application servers in the cluster run from a pair of similarly specced Dell Power Edge R420 database servers, each equipped with four 146GB 15K SAS drives in a RAID 10 configuration and a Dell R410 file server with four 300GB SAS drives.
"We're proud to be able to take care of eCommerce clients of all sizes with maximum performance, up to and including the largest enterprise clients," Chris Wells, president and CEO of Nexcess said in a statement. "Coupled with our long experience in Magento hosting, the in-house expertise we've developed over the years, and our phenomenal support team, we can confidently say that Nexcess should be first on the list for anyone that's serious about hosting a Magento site right."


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Martin del Campo Becerra, Gustavo and Reigber, Andreas and Nannini, Matteo (2018) Feature Enhanced SAR Tomography Reconstruction Through Adaptive Nonparametric Array Processing. In: International Geoscience and Remote Sensing Symposium (IGARSS). International Geoscience and Remote Sensing Symposium (IGARSS), 2018-07-22 - 2018-07-27, Valencia, Spain. doi: 10.1109/IGARSS.2018.8518667.
Full text not available from this repository.
Synthetic aperture radar (SAR) tomography (TomoSAR) employs array signal processing techniques, in order to estimate the location of the vertical structures that compose the backscattering field, in the direction perpendicular to the line-of-sight (PLOS). Due to the limited number of tracks for practical TomoSAR sensing scenarios, it becomes challenging to accurately estimate the source parameters. Additionally, irregular sampling and non-uniform acquisition constellations introduce artifacts and increase ambiguity. The usage of super-resolved parametric methods and compressed sensing (CS) based approaches, improve the vertical resolution and mitigate the effect of sidelobes. However, parametric approaches have the main drawback related to the assumption that the scene is composed by a known finite number of point-type backscattering sources. Also, the CS-based techniques regularly imply a considerable computational burden. Overcoming the disadvantages of the above mentioned TomoSAR-adapted methods, this paper presents a novel non-parametric iterative approach for feature enhanced SAR tomography, in the context of maximum likelihood (ML) estimation theory. The feature enhancing capabilities of the proposed technique are corroborated via processing L-band airborne TomoSAR data of the German Aerospace Center (DLR), acquired by the F-SAR system over the forested test site of Froschham, Germany, in 2017.
|Item URL in elib:
|Document Type:
|Conference or Workshop Item (Lecture)
|Feature Enhanced SAR Tomography Reconstruction Through Adaptive Nonparametric Array Processing
|July 2018
|Journal or Publication Title:
|International Geoscience and Remote Sensing Symposium (IGARSS)
|Refereed publication:
|Open Access:
|Gold Open Access:
|In SCOPUS:
|In ISI Web of Science:
|Maximum likelihood (ML), power spectrum pattern (PSP), spectral analysis (SA), synthetic aperture radar (SAR) tomography (TomoSAR).
|Event Title:
|International Geoscience and Remote Sensing Symposium (IGARSS)
|Event Location:
|Valencia, Spain
|Event Type:
|international Conference
|Event Dates:
|2018-07-22 - 2018-07-27
|IEEE Geoscience and Remote Sensing Society (GRSS)
|HGF - Research field:
|Aeronautics, Space and Transport
|HGF - Program:
|HGF - Program Themes:
|Earth Observation
|DLR - Research area:
|DLR - Program:
|R EO - Earth Observation
|DLR - Research theme (Project):
|R - Aircraft SAR
|Institutes and Institutions:
|Microwaves and Radar Institute > SAR Technology
|Deposited By:
|Martin del Campo Becerra, Gustavo
|Deposited On:
|16 Apr 2018 08:47
|Last Modified:
|25 Jul 2023 11:47
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Effective abandonment depends on knowledge of the well construction, geology and the hydrogeology. The importance of a full characterization increases as the complexity of the well construction, site geology and the risk of aquifer contamination increases. Construction information for wells drilled since 1966 may be available from the Bureau of Topographic and Geologic Survey's (BTGS) Water Well Inventory System database. Additional well construction data and information describing the hydrologic characteristics of geologic formations may be available from reports published by BTGS and the United States Geological Survey. Site or program records also may exist. The well should be positively identified before initiating the abandonment, and field information should be compared with any existing information.
Water levels and well depths can be measured with a well sounder or weighted tape measure. In critical situations, well construction details and hydrogeology can be determined with borehole geophysics or a downhole camera. For example, a caliper log, which is used to determine the borehole diameter, can be very helpful in locating cavernous areas in open hole wells.
If possible, the borehole must be cleared of obstructions prior to abandonment. Obstructions such as pumps, pipes, wiring and air lines must be pulled. Well preparation also may involve fishing obstacles out of the borehole. An attempt should be made to pull the casing when it will not jeopardize the integrity of the borehole. Before the casing is pulled, the well should be grouted to near the bottom of the casing. This will at least provide some seal if the well collapses after the casing is pulled.
The presence of nested or telescoped casing strings complicates well abandonment. Inner strings should be removed when possible, but only when removal will not jeopardize the abandonment of the well. If inner strings cannot be removed and sealing of the annular space is required, then the inner string should be vertically split (plastic cased wells) or cut (metal-cased wells) at intervals necessary to ensure complete filling of the annular space.
Damaged, poorly constructed or dilapidated wells may need to be re-drilled in order to apply proper abandonment techniques. Also, in situations where intermixing of aquifers is likely, the borehole may need to be re-drilled.
Materials and MethodsAggregate -Materials that eliminate the physical hazard and open space of the borehole, but do not prevent the flow of water through the well bore, are categorized as aggregate. Aggregates consist of sand, crushed stone or similar material that is used to fill the well. Aggregates should be uncontaminated and of consistent size to minimize bridging during placement.
Aggregate usually is not placed in wells smaller than two inches in diameter. Nominal size of the aggregate should be no more than one-fourth of the minimum well diameter through which it must pass during placement. Because aggregate usually is poured from the top of the well, care must be taken to prevent bridging by slowly pouring the aggregate and monitoring the progress with frequent depth measurements.
Aggregates may be used in the following circumstances:
- There is no need to penetrate or seal fractures, joints or other openings in the interval to be filled.
- A watertight seal is not required in the interval to be filled.
- The hole is caving.
- The interval does not penetrate a perched or confined aquifer.
- The interval does not penetrate more than one aquifer.
If aggregate is used, a casing seal should be installed. The use of aggregate and a casing seal must be consistent with the future land use.
Sealants - Sealants are used in well abandonment to provide a watertight barrier to the migration of water in the well bore, in the annular spaces or in fractures and openings adjacent to the well bore. Sealants usually consist of portland cement-based grouts, bentonite clay or combinations of these substances. Additives frequently are used to enhance or delay specific properties such as viscosity, setting time, shrinkage or strength.
Sealing mixtures should be formulated to minimize shrinkage and ensure compatibility with the chemistry of the ground water in the well.
A grout pump and tremie pipe are preferred for delivering grout to the bottom of the well. This method ensures the positive displacement of the water in the well, and will minimize dilution or separation of the grout.
If aggregate is to be placed above sealant, a sufficient amount of curing time should pass before placing the aggregate above the seal. Curing time for grout using Type 1 cement typically is 24 hours to 48 hours, and 12 hours for Type III cement.
Bridge Seals - A bridge seal can be used to isolate cavernous sections of a well, to isolate two producing zones in the well, or to provide the structural integrity necessary to support overlying materials (and thus protect underlying aggregate or sealants from excessive compressive forces). Bridge seals usually are constructed by installing an expandable plug made of wood, neoprene or a pneumatic or other mechanical packer. Additional aggregate can be placed above the bridge.
Some RecommendationsThe complexity of the abandonment procedure depends primarily on the hydrogeology, geology, well construction and the ground water quality. Four principal complicating factors have been identified; they include 1) artesian conditions, 2) multiple aquifers, 3) cavernous rocks and 4) the threat or presence of contamination. The recommended procedures for abandoning wells will be more rigorous with the presence of one or more complicating factors. The procedures may vary from a simple casing seal above aggregate to entirely grouting a well using a tremie pipe after existing casing has been ripped or perforated.
Casing Seal - The transition from well casing to open borehole is the most suspect zone for migration of water. In order to minimize the movement of water (contaminated or otherwise) from the overlying less consolidated materials to the lower water-bearing units, this zone must be sealed. Generally, this can be accomplished by filling at least the upper 10 feet of open borehole and the lower 5 feet of casing with sealant. The length of open borehole sealed should be increased if extenuating circumstances exist. Such circumstances would include a history of bacterial contamination, saprolitic bedrock or possibly deep fracture zones. Water-bearing zones reported in the upper 20 feet or so of open borehole are indications of fractures and would warrant additional sealant. Casing that is deteriorated should be sealed along its entire length.
If the casing is to be pulled, the sealant used should remain fluid for a period of time adequate for removal of the casing. If the casing is to remain, then whenever feasible, it should be cut off below land surface. After the casing seal discussed above achieves adequate strength, the open casing should, at a minimum, be filled with aggregate. It is strongly suggested that a sealant be used in the upper 2 feet to 5 feet of casing.
Unconfined or Semi-Confined Conditions - When applicable, unconfined wells in non-contaminated areas may be satisfactorily abandoned using aggregate materials up to 10 feet to 15 feet below the ground surface. This would apply mainly to domestic wells, as well as test borings or wells not covered by existing regulations. Monitoring wells that are not covered by specific regulatory programs and are located at sites with no known contamination might be abandoned in this manner. Above the aggregate, the casing seal should be installed. A sealant may be used over the entire depth.
Contaminated Sites - An abandoned, contaminated well often mixes contaminated ground water with uncontaminated ground water. Complete and uniform sealing of the well from the bottom to the surface is required. Therefore, proper well preparation must be done before the well is sealed with a proper sealant.
Cavernous Rocks - Problems can arise when filling wells that penetrate cavernous rock. Although such wells usually are located in carbonate terrain, voids can occur in areas that have been deep mined. Care must be taken to ensure that aggregates and sealants are of a size and consistency to prevent their removal by water flowing in the void. Large voids or high flow velocities warrant placement of a bridge in competent rock over the void. Aggregate and sealants then can be placed above the bridge.
Multiple Aquifers - The main goal in sealing wells that extend into more than one aquifer is to prevent the flow of ground water from one aquifer to another. If no appreciable movement of water is encountered, and there is no threat of ground water contamination, sealing with concrete, neat cement, grout or alternating layers of these materials and aggregate will prove satisfactory. When ground water velocities are high, the procedures for wells with artesian flow are recommended. If alternating plugs (or bridges) and aggregate layers are used, the plugs should be placed in known nonproductive horizons or, if locations of the nonproductive horizons are not known, at frequent intervals.
Flowing Wells - The sealing of artesian wells requires special attention. The flow of ground water may be sufficient to make sealing by gravity placement of concrete, cement grout, neat cement, clay or sand impractical. In such wells, large stone aggregate (not more than 1⁄4 of the diameter of the hole), well packers (pneumatic or other) or wooden plugs will be needed to restrict the flow and thereby permit the gravity placement of sealing material above the zone where water is produced. If plugs are used, they should be several times longer than the diameter of the well in order to prevent tilting. Seals should be designed to withstand the maximum anticipated hydraulic head of the artesian aquifer.
Because it is very important in wells of this type to prevent circulation between water-yielding zones, or loss of water to the surface or to the annular spacing outside of the casing, it is recommended that pressure grouting with cement be done using the minimum volume of water during mixing that will permit handling.
In wells in which the hydrostatic head producing flow to the surface is low, the movement of water may be stopped by extending the well casing to an elevation above the artesian pressure surface.
Multiple Complications - Wells with one or more of the above complicating factors that are to be abandoned in areas with contaminated ground water or in areas where the ground water is at a high risk for future contamination, require the most rigorous abandonment procedures. In general, the entire length of these wells should be sealed.
When the threat of contamination has been established, the elimination of a potential flow path is critical. For example, a contaminated well in a karst terrain must be sealed carefully to avoid worsening the situation. In general, the entire lengths of these wells should be sealed. In some situations, a bridge seal may have to be installed, and casing may have to be perforated. In each case, a prudent method should be selected that will eliminate all potential vertical flow paths.
Monitoring Wells - Monitoring wells should be abandoned in accordance with the rules and regulations of the program under which they were installed and operated.
Monitoring wells that were installed and continue to function as designed usually can be abandoned in place. Exceptions would include wells whose design precludes complete and effective placement of sealant and wells in locations subject to future disturbance that could compromise the abandonment. In such instances, all tubing, screens, casings, aggregate, backfilling and sealant should be cleaned from the boring, and the hole should be completely filled with an appropriate sealant.
Monitoring wells that are abandoned in place should be completely filled with sealant. Screened intervals can be backfilled with inert aggregate if sealant will alter the ground water chemistry and thereby jeopardize ongoing monitoring at the facility. Intervals between screens, and between the last screen and the surface, must be filled with sealant. Generally, sealant must be emplaced from the bottom of the interval being sealed. Protective casings, riser pipes, tubing and other appurtenances at the surface that could not be removed should be cut off below grade after the sealant has properly set. When the abandonment will be completed below the finished grade, the area of the boring should be covered with a layer of bentonite, grout, concrete or other sealant before backfilling to grade.


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This Paradise land property is located to the north of the country in the province of Alajuela. Rio Celeste or "Light blue river" in English is a canton that was given its name by the exquisite color of the river's water.
The light blue color of its waters is not only a great tourist attraction but is due to the chemical reactions of the calcium carbonate and the sulfur of the Tenorio Volcano. Besides its incredible color, this river also has an amazing waterfall, one of the most beautiful in Costa Rica, and natural hot springs within the majestic tropical forest.
This magnificent property is more than 7 hectares and one of the most beautiful properties around Rio Celeste area, the land has an appraisal of $690 000 but they will sell it for $600 000.
It was declared last year as one of the "7 wonders of Costa Rica", and for this reason there is a high growth of tourism in this area.
The property has 300 meters of water frontage that runs fast enough to create energy.jkvci
Updated on February 14, 2020 at 3:28 pm
One of the 7 wonders of Costa Rica Can be yours - Cantón Guatuso, Alajuela Province, Costa Rica
One of the 7 wonders of Costa Rica Can be yours - Cantón Guatuso, Alajuela Province, Costa Rica


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Place your bets.
You never know to what extent a tuned engine will impact a drag race. So before assuming the Aventador will walk all over the Porsche 911 Turbo, consider that the sporty German has received a G-Force Stage 2 remap, and now delivers 600 horses, up from the stock model's 520 hp. That would have probably evened the playing field, but the Raging Bull here has also been messed with: a Capristo Stage 1 tune helping it to deliver 730 hp, up from 700.
Even with a slower reaction time, the Italian exotic reaches every stage – from 60 feet to 1,000 meters – ahead of the Porsche. But it's surprisingly close up to the quarter-mile. Check it out.


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- Semi-quantitative analysis of the number of repetitions of the GAA expansion of the FXN gene by PCR.
- Amplification range of 100-500 bp.
- Includes TP-PCR systems to detect described expansions leading to amplicon sizes >500 bp.
- It complies with the quality requirements specified by ISO 9001 and ISO 14001 in relation to the materials used in its manufacturing process.
- For research use.
Detection of GAA expansion in the FXN gene associated with Friedreich's ataxia (FRDA)
12 rxn
Fragment Analysis
Compatible equipment
3730xl DNA Analyzer (ThermoFisher Scientific)
Imegen-Friedreich allows analysis of GAA triplet expansions in the FXN gene associated with Friedreich's ataxia by conventional PCR and capillary electrophoresis. In addition, the kit offers a TP-PCR (triple repeat primed PCR) system to detect expansions not detectable with conventional PCR.
- Amount of DNA needed: 50 ng total
- Sample type: Peripheral blood (Germinal)
- Number of reactions per sample: 2
- Number of targets: 1
- Manual work time: 45 minutes
- Duration of the PCR/TP-PCR program: 2 h 15 min
- Compatible thermal cyclers: Any conventional thermal cycler
- Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM)
Fill in the form to request this kit.Call us at +34 963 212 340
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Home » Myanmar Publications » Public Private Partnerships for Myanmar Toll Roads Event held in Tokyo
Public Private Partnerships for Myanmar Toll Roads Event held in TokyoJune 28, 2018
VDB Loi held a workshop in Tokyo titled "Public Private Partnership for Myanmar Toll Roads". There were more than 30 participants from developer companies, construction companies and banks. Edwin highlighted the key points to know when structuring a PPP project in Myanmar. Discussions were held about the prospects of road transportation projects in Myanmar.
Topics discussed also included the Yangon Elevated Expressway, possible structures and commercial terms of upcoming PPP projects in Myanmar, lessons learned from toll road PPP projects in the region, and the contents of a Myanmar toll road PPP Concession Agreement.
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text: US hotels saw 6% growth in direct bookings, online and mobile, in Q216News / DistributionBy Sean O'Neil | August 17, 2016Share This article was originally published on TravelClick, the technology specialist with 38,000 hotel clients worldwide, has released data on how individual leisure and business travelers made hotel reservations in the US during the second quarter of 2016.Direct, or "brand.com", bookings were up 6% in the second quarter of 2016, compared with the second quarter of 2015. That rise represents somewhat higher reservations growth than the 3.3% growth rate for bookings made through online travel agents (OTAs, like Expedia and Booking.com) during the same period, compared with a year earlier.In-person travel agents (using global distribution systems) experienced the most growth in bookings during the second quarter: up 7.4%, compared with second quarter 2015.TravelClick says that if current trends hold, it expects to report more growth when the third quarter is complete, especially for the brand.com channel.Based on actual reservations for the third quarter, 33% of reservations are in the brand.com channel, 20.5% are direct to the hotel, 12.2% are via an agent and a travel agent's global distribution system, and 21.6% are via OTAs. (Calls to a hotel's 800-number took up the remaining share.)As usual, the statistics can be interpreted in numerous ways. But the numbers are likely to be greeted by some hoteliers as a positive sign that their efforts to push more travelers into booking direct are having an effect. For example, Hilton has been running a 'Stop Clicking Around' campaign that it says is its biggest in 97 years, promising discounts for direct bookings as a way to cut out middleman fees.The broad picture is still hazy. In the second quarter, Expedia said it had no hit from hotel chain direct booking campaigns, and major hotel chain quarterly reports presented a mixed picture.It's not all growth. Older methods of making reservations are fading away.Earlier:Expedia's view: Publicly available loyalty rates may be bad for hotel ownersKalibri Labs points hotels toward the true cost of guest acquisitionTravelClick debuts a media suite, revamps its booking engine, and looks at reputation managementNB: Image via Rawpixel.com/BigStock.com


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After I got to Nellis AFB, NV in Jan. '69, I was assigned to the 430th Tactical Fighter Squadron (TFS) (F-111A) as a Weapons Load Officer. One of my enlisted supervisors was TSgt. Leon Jones. Lee was a full-blooded Cherokee Indian, and very proud of it.
One morning we had a 'Mobility Exercise.' This is an exercise where we had to show up at the squadron with all required our deployment materials. Our deployment bags (with 2 weeks of clothes), shot records, dog tags, and so forth. They had a long table set in in our hanger where we 'processed.' I was in line right behind Leon.
When Lee got to the dog tag guy he discovered his tags were out of date – for whatever reason. No worries, they had the dog tag stamping machine right there. When they asked for his religious preference he replied "Heathen." At first they didn't realize he was a Cherokee Indian. But they went ahead and made it for him anyway.
I thought this was pretty cool, so I claimed I lost mine. (2nd Lts. can get away with damn-near anything – "they" think we are all dumb-shits!) So, when I was asked my religious preference, why not? "Heathen." Thinking that was "cool" lasted only until I got to the Chaplain's station! Ever had your ass chewed by a Chaplain? Not fun!
And so now, in 2013, I have had replica dog tags made with "Heathen" on them as my religious preference…. but for a totally difference reason.
I was raised in the Lutheran church, and still consider that my "religion." But my "spirituality" is more aligned with the Native American, or Indian spirituality I have embraced since sobriety. Nature based, non-biased, not a great deal of "dogma," – it just speaks to me so much more than the Lutheran church does these days. And I am so comfortable with it… and I am at Peace.


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Turtle-Stingray trips by boat
- Price: 45€ per person (minimum of 4 people).
- Includes: boat, equipment, instructor.
- Price (minimum of 2 people):
- – Without scooter: 35€ per person
- – With scooter: 75€ per person
- Includes: all equipment and instructor.
- Requirements: have minimum freediving license level 1, insurance (possible to purchase in our center).
Underwater shooting Service and Logistic
Apnea Canarias has collaborated in the realization of many projects such as documentaries, short footage, advertisements, photos, television programs, underwater.
We manage for you the logistic of boats, underwater material, permis, underwater safety, emergency plan and scouting the different spots and we collaborate with professional underwater video maker and photographer.


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Facet Joint Ablation
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Doctors usually try to treat back pain using conservative methods like medication and physical therapy. However, these methods do not always provide the relief you're hoping for. In this kind of situation, you may need surgery.
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Call our team at iRISE Spine and Joint by dialing (888) 966-9750 today. We are committed to helping you however we can.
What Is Facet Joint Ablation?
Facet joints are part of the vertebrae in your spine. Their job is to provide range of motion so you can bend and twist your back. Each of these joints has small nerve endings that communicate signals between the joints and your brain. One of these signals is pain.
The goal of facet joint ablation is to sever this pain signal by burning the nerve endings in the facet joints with electricity. Facet joint ablation does not involve the use of any incisions. Instead, the surgeon will use a thin needle to pinpoint the nerve ending with guidance from X-ray imaging.
The surgeon can transmit a small radiofrequency current through the needle once the correct nerve ending is located. After this, the needle is removed, a bandage is placed over the incision point and the procedure is complete.
What Are the Benefits of Facet Joint Ablation?
Facet joint ablation offers many advantages since it's a minimally invasive procedure.
These advantages include:
- It's quick: The procedure is usually finished within an hour or two. Many patients can return home the same day. However, some may need to stay overnight to monitor their condition.
- It's less painful: Since the procedure involves no incisions through the muscles and soft tissue, there is little pain during recovery. The procedure itself isn't too painful either. That's why it can usually be performed using sedation instead of anesthesia, which comes with risks.
- It produces long-term results: People who undergo facet joint ablation can regain range of motion and live life with less back pain for a long time after the procedure.
iRISE Spine and Joint Is Here to Help
If you're suffering from arthritis or another condition causing facet joint pain in your spine, you should talk to one of our surgeons about a facet joint ablation procedure. If conservative treatment methods aren't treating your pain, this procedure may be your best option. Contact us today to learn more about facet joint ablation or to schedule an appointment.
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Tenascin-C (TNC) is a hexameric, multimodular extracellular matrix protein with several molecular forms that are created through alternative splicing and protein modifications. It is highly conserved amongst vertebrates, and molecular phylogeny indicates that it evolved before fibronectin. Tenascin-C has many extracellular binding partners, including matrix components, soluble factors and pathogens; it also influences cell phenotype directly through interactions with cell surface receptors. Tenascin-C protein synthesis is tightly regulated, with widespread protein distribution in embryonic tissues, but restricted distribution of tenascin-C in adult tissues. Tenascin-C is also expressed de novo during wound healing or in pathological conditions, including chronic inflammation and cancer. First described as a modulator of cell adhesion, tenascin-C also directs a plethora of cell signaling and gene expression programs by shaping mechanical and biochemical cues within the cellular microenvironment. Exploitment of the pathological expression and function of tenascin-C is emerging as a promising strategy to develop new diagnostic, therapeutic and bioengineering tools. In this Cell Science at a Glance article and the accompanying poster we provide a succinct and comprehensive overview of the structural and functional features of tenascin-C and its potential roles in developing embryos and under pathological conditions.
Tenascin-C (TNC) is the founding member of the tenascin gene family. Discovered independently by researchers studying glioblastomas, the myotendinous junction and the developing central nervous system (Chiquet-Ehrismann and Tucker, 2011), its name reflects two sites where tenascin-C is prominently expressed: tendons (from Latin verb 'tenere' to hold) and embryos (from Latin 'nasci' to be born) (Chiquet-Ehrismann et al., 1986). The 'C' – derived from the previous name cytotactin – was assigned when additional members of the tenascin gene family were identified.
Tenascin-C exhibits a number of remarkable features that are related to its structure, expression and function. All tenascins share the same molecular architecture. Heptad repeats near the N-terminus of each tenascin chain support trimerization; in tenascin-C, two trimers can join to form a hexabrachion. The heptad repeats are followed – from N- to C-terminus – by one or more epidermal growth factor (EGF)-like domains, a string of fibronectin-type III (FNIII) repeats and a fibrinogen-like globe (see poster). This multimodular structure enables the tenascins to interact with a high number of highly diverse ligands. Another striking feature of tenascin-C is its tightly controlled gene expression pattern. In the embryo, tenascin-C is found around motile cells, at sites of epithelial-mesenchymal interactions and branching morphogenesis, and in dense connective tissue, such as bone, cartilage and tendons, as well as in the central nervous system (Akbareian et al., 2013; Sahlberg et al., 2001; Wiese and Faissner, 2015). However, little tenascin-C is found in the adult, where it is most prominent in stem cell niches and tendons (Chiquet-Ehrismann et al., 2014), and at sites of inflammation and trauma (Midwood et al., 2011); it is also particularly abundant in the stroma of solid tumors (Brösicke and Faissner, 2015; Lowy and Oskarsson, 2015; Midwood et al., 2011; Orend et al., 2014). Finally, tenascin-C exhibits an extraordinarily wide range of functions. Tenascin-C knockout mice show abnormalities regarding their behavior, brain cytoarchitecture, branching morphogenesis, stem cell niches and trauma responses (Chiquet-Ehrismann and Tucker, 2011). In vitro studies have also shown that tenascin-C can influence cell behavior in a number of ways. It is perhaps best known as an adhesion-modulating extracellular matrix protein owing to its ability to bind to fibronectin, and to alter cell spreading and signaling mediated by fibronectin and integrins (Chiquet-Ehrismann et al., 1986; Huang et al., 2001; Midwood et al., 2004). However, tenascin-C itself also acts directly as an integrin ligand (Tucker and Chiquet-Ehrismann, 2015; Yoshida et al., 2015), as well as performing a whole host of other functions.
The complex interplay of tissue-specific interactions and functions makes it difficult to define the actions of tenascin-C because it possesses an exquisite context-specific mode of action that defies neat classification. Most often identified at disease sites, tenascin-C has been implicated in the pathogenesis of a large number of conditions. However, tenascin-C evolved in early chordates (see poster and Box 1), suggesting a highly selective pressure for this molecule, thereby raising the questions: why do we have tenascin-C and what good does it do? In this Cell Science at a Glance article and its accompanying poster, we summarize many of the features of tenascin-C, including its appearance during evolution, its structure and binding partners, and the regulation of tenascin-C gene expression. We also highlight its roles under a number of physiological and pathological conditions before concluding with future directions for tenascin-C research (see poster and Box 2).
Prior to the ability to analyze genomic sequences, a number of attempts were made to identify tenascins in non-chordate model organisms by screening cDNA libraries with antibodies or nucleic acid probes. Although this approach did result in the serendipitous discovery of the teneurin family of transmembrane proteins (Baumgartner and Chiquet-Ehrismann, 1993; Baumgartner et al., 1994), it was doomed to fail because tenascins are only found in chordates (Tucker and Chiquet-Ehrismann, 2009). The phylum Chordata is usually subdivided into the cephalochordates, tunicates and craniates. The cephalochordates, the subphylum to which the Florida lancelet (also known as amphioxus) Branchiostoma floridae belongs, are widely regarded to be the first chordates to have evolved. A predicted tenascin is found in the B. floridae genome and it has all of the characteristics of tenascins found in vertebrates (Tucker and Chiquet-Ehrismann, 2009). At least seven of the FNIII repeats of this tenascin have an RGD tri-peptide motif within the same exposed loop in which integrin-binding RGD motifs are found within fibronectin and human tenascin-C, making this tenascin likely to be an integrin ligand. To date, there is no evidence of a tenascin or tenascin-like precursor in the genomes of echinoderms or hemichordates, the two phyla that are most closely related to the chordates. In vertebrates, the glycoprotein fibronectin is an important binding partner of tenascin-C, but the genome of B. floridae lacks a fibronectin gene. This suggests that the first tenascins have evolved as independent integrin ligands and not as fibronectin modulatory proteins. Thus, although tenascins evolved early in the chordate lineage, current evidence suggests that tenascin-C first appeared in jawed vertebrates (Adams et al., 2015).
Tenascin-C gene expression is often used as a read-out for successful tissue repair, and has also been used in approaches of tissue-repair and stem-cell-based tissue replacement. For example, incorporation of an integrin-α7β1-activating tenascin-C peptide into bioengineered gels supports triggered neurite outgrowth and osteogenic lineage differentiation (Berns et al., 2016; Mercado et al., 2004; Sever et al., 2014). Similarly, when tenascin-C is used to coat modified platinum coils placed into experimentally injured arteries, the coils are more effective at inducing vessel repair (Miura et al., 2016). Other biomaterials enriched in tenascin-C may also be useful to enhance mesenchymal stem cell survival during tissue repair (Rodrigues et al., 2013). Mutations and polymorphisms in the tenascin-C gene have been implicated in hearing loss (Zhao et al., 2013) and tendinopathies (Saunders et al., 2013), respectively, a fact that might be useful in the diagnosis of these conditions. As tenascin-C is a neoantigen in cancer and rheumatoid arthritis, there is scope to profile serum antibodies that recognize tenascin-C (Mock et al., 2015; Schwenzer et al., 2015) and to exploit tenascin-C as an antigen for immunization. Indeed, amongst 11 highly immunogenic peptides that have been delivered within an immunization cocktail to glioblastoma patients (clinical trial phase I), one represented tenascin-C (Dutoit et al., 2012; Rampling et al., 2016). Moreover, aptamers and antibodies that specifically bind to tenascin-C were applied to deliver drugs or immune stimulatory chemokines, such as IL2, into the tumor microenvironment of patients with different cancers (Catania et al., 2015; Gutbrodt et al., 2013 ; reviewed in Spenle et al., 2015b). Furthermore, tenascin-C gene expression negatively influences the cytotoxicity of doxorubicin in breast cancer and melanoma spheroids, as well as pancreatic cancer cells (Fukunaga-Kalabis et al., 2010; Oskarsson et al., 2011), suggesting that tenascin-C is a good target for adjuvant chemotherapy. Accordingly, downregulation of tenascin-C gene expression has also been a goal of RNA-based technologies (Rolle et al., 2010). In addition, anti-tenascin-C antibodies coupled to radioisotopes might be useful to image tissues and tumors of high tenascin-C gene expression (reviewed in Gu et al., 2015; Jacobson et al., 2015; Orend et al., 2014; Spenle et al., 2015b). Detection of high protein levels of tenascin-C within pathological tissues as well body fluids could be of diagnostic value and might provide a means to earlier detect associated conditions, including heart disease, fibrosis, pre-eclempsia, rheumatoid arthritis, systemic lupus erythematosus and cancer (Orak et al., 2016; Orend et al., 2014; Schwenzer et al., 2015; Ulusoy et al., 2015, Franz et al., 2015, Imanaka-Yoshida, 2012). Finally, the high levels of tenascin-C found in breast milk of HIV-infected mothers can potentially be exploited in a HIV-prevention strategy 'designed by nature' as their breast-fed infants are protected from contracting HIV (Fouda et al., 2013).
The many molecular variants of tenascin-C
Tenascin-C can exist in a number of different isoforms that are created by modifications occurring between transcriptional to post-translational levels (see poster). In human tenascin-C, eight of the FNIII repeats (FNIII 1–8) are always present, but the nine FNIII repeats located between FNIII 5 and FNIII 6 are subject to alternative splicing (FNIII A–D), potentially giving rise to 511 different isoforms. This complexity has significant impact on tenascin-C function because distinct FNIII repeats have specific binding partners, proteolytic cleavage sites and post-translational modification sites. Furthermore, new binding sites can be created that exist only across the boundaries of specific FNIII repeats. Splicing of tenascin-C can be extraordinarily dynamic, and has been best studied during development and in cancer, where the temporal and spatial expression of different isoforms is used to direct distinct cellular processes that drive embryogenesis and tumorigenesis (Giblin and Midwood, 2015).
Tenascin-C contains several predicted glycosylation sites (see poster), and tenascin-C purified from human glioma cells and chicken embryonic fibroblasts or that secreted in human breast milk is glycosylated. However, the extent and pattern of modification, and how this impacts tenascin-C function, is not well characterized. One study reports that glycosylated tenascin-C promotes proliferation of murine neuronal stem cells (Yagi et al., 2010). However, this modification is also expected to regulate the binding capabilities of tenascin-C, confer protection from proteolytic cleavage and/or interfere with hexamer assembly (Giblin and Midwood, 2015). Tenascin-C can also be modified post-translationally through citrullination, where a positively charged arginine residue is converted into a neutral citrulline residue. Five distinct citrullination sites were identified within the fibrinogen-like globe, with additional sites likely to be distributed throughout the molecule (see poster). This modification increases the immunogenicity of the C-terminal residues of tenascin-C, leading to the generation of autoantibodies in patients with rheumatoid arthritis, where their presence accurately predicts disease development (Schwenzer et al., 2015).
Finally, tenascin-C can be cleaved by matrix metalloproteases (MMPs) (Giblin and Midwood, 2015) and gingipain cysteine proteases (Ruggiero et al., 2013), which contributes to tenascin-C turnover in tissue. In vitro cleavage of purified tenascin-C through MMP-1,-2, -3 and -7 demonstrated that the majority of cleavage sites are located within the alternatively spliced domains, although MMP-7 and the proteinase ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5) both also cleave within the assembly domain. Although degradation is an important mechanism to control the levels of tenascin-C in tissue, it can also pro-actively impact on tenascin-C function by releasing soluble fragments that have unique functions compared to the full, intact molecule. For example, cleavage can uncover cryptic pro-apoptotic activity, hidden fibronectin-binding sites and concealed heparin-sulphate-binding sites that promote cell spreading (Giblin and Midwood, 2015).
The cellular and molecular interactions of tenascin-C
Tenascin-C binds to a high number of different ligand types (see poster). It interacts with other extracellular matrix (ECM) components – the first and best characterized of which is fibronectin – through a number of binding sites that are distributed throughout its FNIII repeats. Tenascin-C also binds to collagen, periostin and fibrillin-2 – although binding sites for these molecules have not yet been mapped – as well as to proteoglycans of the lectican family and to perlecan, which use binding sites within FNIII 3–5 and the fibrinogen-like globe (Orend et al., 2014). Tenascin-C also binds to receptor-type protein tyrosine phosphatase beta zeta (RPTPβζ), contactin, CALEB (officially known as CSPG5) and glypican; the binding maps have been published elsewhere (Midwood et al., 2011). These inter-matrix interactions are likely to play a structural role – for example, by defining the stiffness of the cellular environment – but they can also modulate how ECM components signal to the cell. Interactions of tenascin-C with cell surface receptors enable direct communication with the cell. Human tenascin-C binds to six different integrins through its RGD-containing FNIII 3 repeat, but integrin-binding sites are also located in the alternatively spliced FNIII repeats and the fibrinogen-like globe (Tucker and Chiquet-Ehrismann, 2015; Yoshida et al., 2015). Some controversy about integrin specificity has been discussed elsewhere (Tucker and Chiquet-Ehrismann, 2015). Tenascin-C can also impact on cell proliferation through activation of the epidermal growth factor (EGF)-receptor (Swindle et al., 2001) and inflammatory signaling pathways through activation of Toll-like receptor TLR-4 (Midwood et al., 2009). More recently, tenascin-C interactions with soluble factors have emerged, e.g. with Wnt/wingless 3a (Wnt3a), transforming growth factor beta (TGFβ) and vascular endothelial growth factor (VEGF); many growth factors use binding sites located in FNIII repeats 3–5. This sequestration of soluble factors might prolong their half-life, increase their local concentrations and/or affect their conformation, all of which will affect their ability to signal to the cell (De Laporte et al., 2013; Hendaoui et al., 2014; Martino et al., 2014). Finally, tenascin-C can bind to pathogens, including Streptococcus and human immunodeficiency virus (HIV); here, tenascin-C may serve as a conduit for, or structural barrier to, infectious agents, and/or may actively participate in host defence. For example, tenascin-C binding to the HIV-1 chemokine receptor site neutralizes viral activity (Fouda et al., 2013).
Tenascin-C in tissues
In contrast to other ECM glycoproteins, tenascin-C has a restricted protein distribution that can rapidly change. In embryos, tenascin-C appears at many sites of tissue morphogenesis (Chiquet, 1992). For example, in limb buds, tenascin-C is a marker for organization of the perichondrium and periosteum, ligaments and tendons (Chiquet and Fambrough, 1984). Tenascin-C also accumulates around budding epithelia in developing kidneys, lung, mammary glands, teeth and hair follicles (Chiquet, 1992), and is expressed around neurons and glial cells in some regions of the developing central nervous system (Faissner, 1997). In the normal, healthy adult organism, tenascin-C expression is sparse; it is found only in a few connective tissues that bear tensile strength (Flück et al., 2000), underneath some epithelia (Aufderheide et al., 1987; Talts et al., 1997) and in certain stem cell niches – including the bulge of hair follicles, crypts of the intestine and bone marrow (Chiquet-Ehrismann et al., 2014).
The most intriguing aspect of tenascin-C protein synthesis is its rapid induction in many tissues in response to pathological stress (Chiquet-Ehrismann and Chiquet, 2003). For example, patients with high levels of C-reactive protein, which is elevated upon infection and inflammation, also have high serum levels of tenascin-C (Schenk et al., 1995). Furthermore, tenascin-C appears after mechanical or chemical injury, including mechanical overload of muscle and tendons (Flück et al., 2000). Stromal cells (e.g. myeloid cells in cancer tissue) are the main tenascin-C source after injury, although inflammatory leukocytes also contribute (Goh et al., 2010; O'Connell et al., 2011). Indeed, tenascin-C is induced during chronic inflammation, for instance in rheumatoid arthritis; (Midwood et al., 2011). Moreover, many articles have described increased protein levels of tenascin-C in human cancers (reviewed in Lowy and Oskarsson, 2015, Orend et al., 2014). Tenascin-C protein synthesis is often used as a readout for successful tissue repair, and has also been used in tissue repair and stem-cell-based approaches of tissue replacement. Increased levels of tenascin-C in chronic inflammation and cancer can also be exploited for diagnosis and targeting (see poster and Box 2).
Regulation of Tnc gene expression
As expected for highly regulated genes, the promoter of the tenascin-C gene (Tnc) contains a TATA box (Chiovaro et al., 2015), and a number of specific transcription factors that contribute to driving the distinct patterns of Tnc expression in the embryo and the adult. For instance, the product of the patterning gene paired mesoderm homeobox protein 1 (Prrx1) directly regulates the Tnc promoter (Martin et al., 1995); it transactivates the mouse Tnc gene by interacting with a conserved homeodomain-binding sequence in its promoter (Ihida-Stansbury et al., 2004). In contrast, homeobox even-skipped homolog protein 1 (Evx1) indirectly stimulates Tnc expression by synergizing with transcription factors Jun and/or Fos (Jones et al., 1992). At least two more homeobox transcription factors, Pou3F2 and Otx2, regulate Tnc expression through direct binding to conserved sequences in its promoter (Copertino et al., 1997; Gherzi et al., 1997). Finally, Tnc was shown to be a target gene of Sox4, which is overexpressed (together with Tnc) in many human tumors (Scharer et al., 2009).
Although de novo expression of Tnc is a hallmark of inflammation, and a number of cytokines were shown to induce the gene (Chiquet-Ehrismann and Chiquet, 2003), it is not yet known how cytokines control Tnc transcription. However, two potential binding sites for SMAD2 and SMAD3 have been shown to be responsible for activation of the Tnc promoter through TGFβ in human fibroblasts (Jinnin et al., 2004). Platelet-derived growth factor (PDGF) regulates Tnc expression via the phosphoinositide 3-kinase (PI3K)–AKT signaling pathway, which induces the binding of transcription factor specificity protein 1 (SP1) and of transcription factors ETS1 and ETS2 to specific promoter sequences (Jinnin et al., 2006). The cell-bound ligands Delta and Jagged have also been shown to induce Tnc through their transmembrane receptor Notch, whereby the cleaved intracellular domain of Notch2 acts in a manner that is dependent on binding sites for transcription factor RBPJk within the Tnc promoter (Sivasankaran et al., 2009).
Moreover, Tnc expression is strongly suppressed through glucocorticoids (Ekblom et al., 1993) but the mechanism is still unknown.
Tnc expression can be regulated by mechanical stress, for instance, in arterial smooth muscle through hypertension (Mackie et al., 1992) or by mechanical overload in skeletal muscle (Flück et al., 2000). In cultured fibroblasts that are subjected to tensile strain, β1-integrin-mediated activation of the small GTPase RhoA induces actin assembly and contraction (Lutz et al., 2010); this results in nuclear translocation of the transcriptional regulator megakaryocytic leukemia-1 (MKL1) to activate Tnc expression (Asparuhova et al., 2009). Alternative mechanotransduction pathways controlling Tnc expression have been discovered in other cell types, for example, in arterial smooth muscle cells, stretch was found to induce the synthesis of the nuclear factor of activated T-cells 5 (NFAT5), a transcription factor that stimulates Tnc transcription (Scherer et al., 2014).
Physiological and pathological cell responses mediated by tenascin-C
Tenascin-C exerts different effects on many different cell types and can stimulate multiple signaling events at once. Cells respond in a context-specific manner to tenascin-C, and tenascin-C often triggers the opposite effect in two cell types (Chiquet-Ehrismann et al., 2011; Midwood and Orend, 2009; Orend et al., 2014). Below, we summarize some of the distinctly different means by which tenascin-C impacts on cell behavior (see also poster).
One of the earliest descriptions of tenascin-C function reported its ability to modulate cell adhesion to other matrix components (Chiquet-Ehrismann et al., 1986). For example, tenascin-C blocks fibronectin-mediated cell adhesion and spreading through competition with syndecan-4 (Huang et al., 2001). Tenascin-C itself can be a poor adhesive substrate for cells, and activation of annexin-II-mediated anti-adhesive signaling through its FNIII repeats A–D renders larger tenascin-C isoforms less adhesive than smaller ones (Giblin and Midwood, 2015). The anti-adhesive properties of tenascin-C can also be enhanced, reduced or even abolished by additional signaling that involves lysophosphatidic acid receptor (LPAR), platelet-derived-growth factor receptor (PDGFR), endothelin type A and B receptors (EDNRA and EDNRB, respectively), or cleavage through proteases (Ambort et al., 2010; Gundersen et al., 1997; Lange et al., 2007, 2008).
ECM networks that are tenascin-C-rich exert control over cellular movement. For example, tenascin-C promotes neural crest cell migration (Akbareian et al., 2013; Tucker, 2001), as well as migration of tumor cells and metastatic progression (Lowy and Oskarsson, 2015; Saupe et al., 2013). However, tenascin-C has also been shown to inhibit cell migration, especially in the case of growth cones (Faissner, 1997). Tenascin-C located in the reticular fibers of the thymus controls lymphocyte influx and appears to define places of leukocyte maturation (Drumea-Mirancea et al., 2006). Similar structures identified as poorly characterized bundles of matrix (matrix tracks) may exist throughout tumors (Kaariainen et al., 2006; Spenlé et al., 2015a) where they could have a role similar to that of reticular fibers in the thymus or other lymphoid tissues (Midwood et al., 2011) (see poster). Tenascin-C is a component of softer regenerative matrix (Chiquet-Ehrismann and Chiquet, 2003), suggesting that this matrix component actively modulates the mechanical properties of the ECM (Miroshnikova et al., 2016). Tenascin-C modulates cellular tension by dismantling actin stress fibers (Midwood and Schwarzbauer, 2002; Ruiz et al., 2004; Saupe et al., 2013). In contrast, tenascin-C promotes constriction of cerebral arteries, which involves activation of EGFR and extracellular signaling-related kinases 1 and 2 (ERK1/2), although it is unknown whether this involves effects of tenascin-C on matrix resilience (Fujimoto et al., 2016).
Tenascin-C promotes survival of oligodendrocyte precursor cells by enhancing fibroblast growth factor receptor (FGFR) and by blocking bone morphogenic protein (BMP4) signaling (Garcion et al., 2004) but has been shown to induce apoptosis in smooth muscle cells through its EGF-like repeats (Wallner et al., 2004). Tenascin-C also promotes and blocks proliferation of tumor cells (Huang et al., 2001) and fibroblasts (Orend et al., 2003), respectively, a process in which tenascin-C and fibronectin compete for binding to syndecan-4 and that, potentially, involves other not-yet-identified mechanisms. In cancer stem cells, tenascin-C promotes proliferation (Fukunaga-Kalabis et al., 2010; Oskarsson et al., 2011), or their transdifferentiation into cells with endothelial properties (Pezzolo et al., 2011).
The high conservation of tenascin-C throughout evolution, its lack of mutational hotspots and its tightly controlled expression all argue for an important role in physiology. However, tenascin-C is also persistently expressed in a high number of pathological conditions (see poster), two well-studied examples being chronic inflammation and cancer. Although Tnc is transiently induced during acute inflammation, its continued expression in several chronic inflammatory diseases has been shown to contribute to disease severity in humans as well as in mice (Udalova et al., 2011).
Hundreds of publications also indicate a role for tenascin-C in promoting cancer progression and metastasis (see poster). For example, a study using a mouse model, in which tumors express tenascin-C either in abundance or not at all, has demonstrated that tenascin-C promotes multiple events, such as survival, proliferation, invasion and metastasis (Saupe et al., 2013); this is consistent with reports studying cancer in humans (reviewed in Orend et al., 2014, Lowy and Oskarsson, 2015). Tenascin-C promotes the angiogenic switch and the formation of poorly functional blood vessels that may involve Wnt signaling (Saupe et al., 2013). Tenascin-C is also a strongly expressed molecule of the AngioMatrix (i.e. ECM and ECM-related proteins that are part of the angiogenic switch), whose expression correlates with worsened patient survival (Langlois et al., 2014). Tenascin-C exerts a dual role in angiogenesis. Whereas tenascin-C blocks pro-survival signaling in endothelial cells through direct contact, it also induces a pro-angiogenic secretome in glioblastoma cells (Rupp et al., 2016). Tenascin-C can also promote survival of circulating breast cancer cells and their colonization within lung parenchyma; this involves Notch and Wnt signaling, including stemness-promoting leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5) (Oskarsson et al., 2011). Synthesis of tenascin-C protein at the invasive front of the tumor and in lung metastases correlates with poorer prognosis (reviewed in Orend et al., 2014). Here, Rho inhibition (through impairing syndecan-4 and integrin α5β1) and activation of Cdc42 and Rac (involving fascin) could be instrumental (reviewed in Lowy and Oskarsson, 2015). Tenascin-C can promote migration through epithelial-to-mesenchymal-transition (EMT) and EGFR activation by the tenascin-C EGF-like repeats (reviewed in Lowy and Oskarsson, 2015; Shao et al., 2015). Finally, through regulating innate and adaptive immunity, tenascin-C can also contribute to tumor progression, although the mechanisms by which this occurs are not completely understood to date (Hauzenberger et al., 1999; Orend et al., 2014; Jachetti et al., 2015).
Conclusions and perspectives
Tenascin-C not only modulates cell behavior by acting as a ligand for cell surface receptors, such as integrins and TLR4, but also has important roles in modulating integrin signaling through fibronectin and by anchoring growth factors. Several potential ligands within the ECM have been found but the nature and purpose of these interactions are mostly unknown. Alternative splicing, post-translational modifications and proteolytic processing of tenascin-C have been identified, yet the distinct functions of the arising tenascin-C molecules are poorly understood. The combined findings summarized here indicate that, in addition to its little understood roles in physiology (e.g. in immunity and EMT), a main function of tenascin-C is that of a stress protein that serves to modulate cell adhesion, migration and proliferation. A better understanding of its roles might lead to the development of new applications in tissue repair, as well as to more appropriate therapies in the fight against chronic inflammation, tumor growth and metastasis. Challenges have to include the development of immune-competent animal models with tightly regulated, tissue-specific protein expression of tenascin-C and several of its more common variants.
This Cell Science at a Glance article is dedicated to Ruth Chiquet-Ehrismann.
G.O. acknowledges support by INSERM, the University of Strasbourg, Institut National du Cancer, Ligue Contre le Cancer, Worldwide Cancer, Association pour la Recherche du Cancer. M.C. was supported by grants from the Swiss National Foundation. K.S.M is funded by Arthritis Research UK, the Kennedy Trust for Rheumatology Research, Asthma UK and Nascient Ltd.
Competing interests
K.S.M. is the founder of, and consultant to, Nascient Ltd.


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This pandemic has created a lot of uncertainty in our lives, mainly in our careers. You might be on edge about job security, rebuilding from a layoff or maybe you are using this opportunity to pivot professionally. Right now, whatever path you're on, you likely will need to build support and momentum for your ideas.
Women have difficulty asking for help. We tend to shy away from outreach to enlist support in our vision and dreams. Keep in mind, the right mentors, advisors or investors can take your professional aspirations to the next level!
I had the pleasure of interviewing Debi Kleiman, award-winning marketer, teacher, startup advisor and author of "First Pitch, How to Win Mentors, Money and More for your Start-up". She offered up some wonderful advice on how to build business relationships even in a Pandemic .
Visit momshierarchyofneeds.com to read the full interview, It's All in the Ask. How to Get the Professional Support You Need Right Now.


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The Other Side of Lost by Jessi Kirby is an emotional story about grief and rediscovering yourself. Following an ex-social media star who takes her cousin's place hiking the perilous John Muir trail, this is a thought-provoking story with a complex main character. In addition to Mari's inspiring journey to re-discover herself, I loved the book's emphasis on friendship. This is a powerful read that is sure to tug at your heartstrings.Title: The Other Side of Lost
Author: Jessi Kirby
Publisher: HarperTeen
Genres: Contemporary, Young Adult Fiction
Publication Date: August 7, 2018
Girl Online meets Wild in this emotionally charged story of girl who takes to the wilderness to rediscover herself and escape the superficial persona she created on social media.
Mari Turner's life is perfect. That is, at least to her thousands of followers who have helped her become an internet starlet. But when she breaks down and posts a video confessing she's been living a lie—that she isn't the happy, in-love, inspirational online personality she's been trying so hard to portray—it goes viral and she receives major backlash. To get away from it all, she makes an impulsive decision: to hike the entire John Muir trail. Mari and her late cousin, Bri, were supposed to do it together, to celebrate their shared eighteenth birthday. But that was before Mari got so wrapped up in her online world that she shut anyone out who questioned its worth—like Bri.
With Bri's boots and trail diary, a heart full of regret, and a group of strangers that she meets along the way, Mari tries to navigate the difficult terrain of the hike. But the true challenge lies within, as she searches for the way back to the girl she fears may be too lost to find: herself.
Review: The Other Side of Lost by Jessi Kirby
After social media influencer Mari breaks down and admits to living a lie, she decides it's time to get away and escape the backlash. In order to unplug, she decides to take her cousin's place on the daunting John Muir hike–the hike they were supposed to do together before Bri passed away. Armed with Bri's diary and meticulous plans, Mari takes on the hike by herself and discovers her true self along the way.
❀ Well-Written Character Arc
I loved Mari's character arc, and she is so well written that I could feel her emotions. At the beginning of the book, Mari is obsessed with social media, but behind her online persona is insecurity. When the thing she has dedicated her life to comes crashing down, she is forced to re-evaluate and confront the parts of herself she has buried beneath the surface. Throughout the book, Mari faces many challenges, both physically and mentally, but I enjoyed how she pushes herself to persevere, despite being a novice hiker who is grieving at the same time.
❀ Focus on Friendship
One of my favourite parts about the book, besides the gorgeous descriptions of nature, is its focus on friendship. The dynamics between Mari and Bri are complicated, and Mari's social media obsession ended up tarnishing her friendship with her cousin. After Bri's death, the regret Mari feels along the hike pierced my heart, but I loved how she is able to connect with Bri again through Bri's journals. This one also contains some fantastic side characters, and I enjoyed getting to know the other hikers Mari meets. The group Mari finds herself hiking with is so supportive, and it is through friendship and genuine connection that she is able to truly blossom.
❀ A Powerful Story
The Other Side of Lost by Jessi Kirby is a powerful story about grief and self-discovery. I loved following the main character as she hikes the John Muir trail, and Mari is such a complex main character. This one also highlights friendship, and I fell in love with the hikers Mari meets on the way. I would definitely recommend this to those looking for a thought-provoking and moving read.


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Bring a large pot of lightly salted water to a boil. Place pasta in the pot, cook for 8 to 10 minutes, until al dente, and drain.
In a large bowl, whisk together the mayonnaise, milk, pickle juice, dill, salt, and pepper. Mix in tuna and onion. Toss with cooked pasta. Cover and refrigerate 1 to 2 hours before serving.
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Merchant designs informational materials and training programs to help reduce pesticide use and promote use of low-impact pesticides for schools and other institutional facilities. He develops training materials and continuing education programs on pests and integrated pest management for the public. His research interests include control of fire ants in electrical equipment and in urban landscapes; low-impact pesticides; spider management; documentation of Formosan termite range expansion in Texas; and improvement of existing termite control technologies.
Areas of Expertise
- Extension urban arthropods/consumer pesticides
- Management of arthropod pests affecting human health
- Integrated pest management systems for institutions
- Landscape pest management
- Pesticide applicator training


text: THIS IS AN AUTHENTIC MOVIE PROP BOOK USED FOR THE OPENING TITLE SEQUENCE OF WARNER BROS' 1949 FILM ADAPTATION OF ROBERT WILDER'S "FLAMINGO ROAD" STARRING JOAN CRAWFORD AND DIRECTED BY MICHAEL CURTIZ. THE COVER IS HAND PAINTED IN POSSIBLY GOUACHE AND HAS MODERATE TO HEAVY WEAR. BINDING IS SOMEWHAT WEAK BUT STILL INTACT AND THE TITLE PAGES ARE STILL ATTACHED. THIS IS AN ORIGINAL ONE OF A KIND ITEM AND NOT A REPRODUCTION OR FACSIMILE.The item "FLAMINGO ROAD AUTHENTIC MOVIE PROP BOOK FOR TITLES JOAN CRAWFORD WARNER BROTHERS" is in sale since Wednesday, July 25, 2018. This item is in the category "Entertainment Memorabilia\Movie Memorabilia\Props\Originals". The seller is "wizzzky" and is located in Los Angeles, California. This item can be shipped to United States, Canada, United Kingdom, Denmark, Romania, Slovakia, Bulgaria, Czech republic, Finland, Hungary, Latvia, Lithuania, Malta, Estonia, Australia, Greece, Portugal, Cyprus, Slovenia, Japan, China, Sweden, South Korea, Indonesia, Taiwan, South africa, Thailand, Belgium, France, Hong Kong, Ireland, Netherlands, Poland, Spain, Italy, Germany, Austria, Bahamas, Israel, Mexico, New Zealand, Singapore, Switzerland, Norway, Saudi arabia, Ukraine, United arab emirates, Qatar, Kuwait, Bahrain, Croatia, Malaysia, Brazil, Chile, Colombia, Costa rica, Panama, Trinidad and tobago, Guatemala, Honduras, Jamaica, Barbados, Bangladesh, Bermuda, Brunei darussalam, Bolivia, Ecuador, Egypt, French guiana, Guernsey, Gibraltar, Guadeloupe, Iceland, Jersey, Jordan, Cambodia, Cayman islands, Liechtenstein, Sri lanka, Luxembourg, Monaco, Macao, Martinique, Maldives, Nicaragua, Oman, Peru, Pakistan, Paraguay, Reunion, Viet nam, Uruguay.


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The Secular Carmelite Order (OCDS) was formerly inaugurated on 15 July, 2007 at our Church. Rev. Fr. John Lobo, the Parish Priest as well as the Spiritual Director of the Order along with other four representatives lighted the lamp and wished that the light lit today may inspire many to dedicate themselves to the Order and may enable many to climb the mountain of God i.e., Jesus the Way, the Truth and the Life. In his words of welcome the Director appreciated the generosity of the participants and briefed about the necessity of joining the Order. Br. Stephen Pereira, the Animator enlightened the minds of the aspirants regarding the Order, the scriptural background, its development, advantages, and the responsibilities of the members. Later, for the better coordination of the Order a president and a secretary were elected. Mr. Patrick Fernandes and Mrs. Esther volunteered to be the President and Secretary respectively. It was decided that the members would gather on the 4th Sunday of every month for a meeting and prayer. The Director thanked the aspirants for dedicating themselves to the Marian Order i.e., Secular Carmelite Order and resolving to reach perfection as Jesus commanded in the Gospel, "Be perfect as your Heavenly Father is perfect" (Mt 5: 48)
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Ideal for removing unsightly hair on the face or body.
Use on the nape of neck, face, ears or bikini line. It's personal.
Less stressful on the skin than waxing or shaving with an ordinary razor. Precision design for those hard to reach places. Comes with a comb attachment which makes it excellent for trimming and thinning eyebrows.
Operated by one AAA battery included in the pack.
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Regular Royal mail Delivery | UK Mainland.
We accept paypal as well as visa and mastar card payments.


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Manchin Welcomes Dozens of West Virginians to First-Ever 'Wednesday Wake-Up With Joe'
Manchin launched weekly coffee hour in his Washington office and invites all West Virginians to attend
Washington, D.C. – U.S. Senator Joe Manchin (D-W.Va.) welcomed dozens of West Virginians to his office this week in his first-ever "Wednesday Wake-Up with Joe" coffee hour for constituents visiting the nation's capital.
"Our first-ever coffee hour was a big success, and I truly enjoyed hearing from all the West Virginians who stopped by my office for a little coffee and common sense," Senator Manchin said. "I hope that all West Virginians will stop in at 'Wednesday Wake-Up With Joe' when they come to our nation's capital, whether it's for work or pleasure. Hearing from the people of our great state truly helps me be the best Senator I can be."
West Virginians can visit the Senator's web site, www.manchin.senate.gov, where they will find a list of dates and times for upcoming coffee sessions. Once at the web site, click on "Contact Joe" and follow the link to the Constituent Coffee Series to RSVP online.
West Virginians can also RSVP by calling Senator Manchin's Washington office at (202) 224-3954 or by emailing firstname.lastname@example.com
Audio of Senator Manchin talking about the first ever "Wednesday Wake-Up With Joe" is available here: https://s3.amazonaws.com/sdmc-media.senate.gov/Manchin/051612_MANCHIN_1_AUDIO.mp3Broadcast quality video is available here:
Broadcast quality video is also available on Pathfire.
Directions for accessing Pathfire are available here: http://democrats.senate.gov/tv/pathfire/manchin.pdf
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- This class has passed.
Defensive Driving Live Course – Columbia County
Saturday August 12 @ 9:00 am - 3:15 pm$45
Each class is six hours and includes a 10-minute morning and a 45-minute lunch break. Classes start promptly at 9:00am and end at 3:15pm in Room 612, Professional Academic Center (PAC).
How to reserve a seat in this class:
Call Columbia-Greene Community College Community Services Office: (518) 697-6370


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From Wildlife Photography
to Trading
After some discussion with Amplify Trading, I did the professional trading course and completed Stage 3 in Apr 19.
What made you choose Amplify Trading?
I did extensive (and occasionally expensive!) research into trader training. Suffice to say that the quality of training provision, by the financial markets in general, is quite poor. I decided to look for training that was backed up by a professional body and came across Amplify Trading via the LIBF. I attended an evening session at their trading floor in the City of London, where they prepared and traded through an FOMC Statement and Press Conference in real-time. I was impressed by their professionalism and openness, after further discussion I signed up for their professional trading course. Previous reviews by former students were also positive, and a few in particular really struck home with me in that they had been where I currently was in my "trading journey".
What was your knowledge before coming on the programme?
Like many people who have traded in the 'retail' sector I had a reasonable grasp of technical analysis. However, I was basically trading too much, just "treading water" with my trading account balance, and not really making any tangible headway. Although I knew this put me in the upper-quartile of all traders, I also knew there were gaps in my trader knowledge.
Attending the live trading session at Amplify Trading opened my eyes to how Fundamental Analysis and Technical Analysis are equally important to master, in order to develop consistency and profitability in trading. Although that is easy to say, it really requires a committed engagement with Amplify Trading in order to grasp and understand how these analysis methodologies coexist both individually and collectively.
How did you find the variety and quality of the training provided by the Amplify team?
The training was first-class and through the stages of the professional trading course I think I had first-hand experience with the entire training team, who are also professional traders and analysts.
Although some lean more towards either Fundamentals or Technicals, it was very clear that they all paid close attention to both aspects in their trading. Although open-minded, they also had preferences on the markets they liked to trade; ie. commodities, indices, forex. There was also professional expertise to hand from traders in the online Live Room, where trades and analysis are freely discussed.
Do you feel you have made improvements in your trading since attending the course?
Time will tell, trading requires focus on the 'long game' and it takes time to demonstrate one's consistency. That said, I now trade less and "sit on my hands" more! The net effect is fewer losses with shallower peaks and troughs on the "equity curve".
I also now have sufficient expertise to trade a Demo account with a sense of purpose, more professionally and in parallel with my Live account. This helps to develop and test trading strategies, as well as being a positive distraction from live trading when the markets are quiet!
What was your previous career and do you think there were any skills/experiences that have helped with your trading?
The major part of my previous career was serving in the military, and then civil security. There are personal traits that helped me during challenging times, and many equally apply in trading: integrity, patience, honesty (with yourself), perseverance, performing under pressure, and tenacity are a few.
There are times when a trading loss might seem to be the end of the world; but there are far worse things in life that can happen, and being able to rationalise, persevere and overcome adversity, are useful skills to have in trading. I also know that a plan can fail to survive "first contact", however having a plan when the pressure is on and knowing what to do when things go wrong is key to survival.
Entering into a trade is easy, and a good grasp of Fundamental & Technical analysis will help identify an entry level for a trade to have a higher probability of success. However, knowing when and where to exit a trade is what really matters, whether it's for a gain or a loss.
When not trading you are travelling the world taking wildlife photography. Does this help you unwind from trading?
Trading can be a bit of a "lone wolf" activity. I am lucky that my wife is also a keen photographer and traveller, so it is something we enjoy together. It helps us unwind from the constraints of a desk-routine; just being outdoors and enjoying nature is a great antidote. I think there is synergy between trading and wildlife photography. Planning, patience and timing springs to mind having spent an hour waiting for 'the shot' and the 'the trade'. Traders often talk about FOMO (Fear of missing out), as do photographers: it requires a plan that is well executed in order to increase the probability of success with some consistency. We recently visited The Pantanal and Amazon in Brazil and spent 2 very long hot and humid days on a 1.5m (5ft) square platform, on top of a 32m (104ft) tower above the tree canopy, hoping to get a shot of the Harpy Eagle (the world's largest eagle). There were times when I almost wished I was at my desk trading …. (only almost). On the flip-side, travelling at 40+ knots up the Cuiaba River to reach a Jaguar sighting certainly blew off some cob-webs.
What would be your advice to anyone interested in trading the financial markets?
You need to accept that you have to commit to the long-game. Like any activity performed at an elite level, the biggest challenge is developing the right state of mind. There is NO fast-track to consistent success; anyone can pick a winner, or a loser. It's all about developing your 'edge' in the market, and executing your plan; Amplify Trading can help you develop the right skill-set and mind-set in order to trade. However the consistency is down to how you, the professional, actually trade.
I found with my group that those who had a basic understanding about a chart, and how to analyse it, with simple line drawing tools to show trends, support and resistance, were able to grasp the Amplify Trading concept much quicker. There is plenty of good information freely available online to get you started. Coupled with Amplify Trading's Youtube channel I found Investopedia and Trading Economics useful web-resources.
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|Springtime in the Rockies
More About This Blog...
Loving my camera on the iPhone and photo processings apps.... Here you will find the results of my personal creativity challenge--posted one picture a day for years-now it happens a lot less. The post processing is done using only iPhone or iPad apps. Explore! Enjoy!
Thursday, May 2, 2013
Spring time in the Rockies
Gotta love it! Snow one day and seventy degrees the next day. It is spring time in the Rockies. One can snow ski in the morning, golf in the afternoon and water ski in the evening. Sweet!
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Fit Tip: Length - true to size. Width - Slightly Wide. Arch - Slightly Low. Little to no break-in.
Leading pickleball footwear and apparel brands K-Swiss and Lucky In Love make history by collaborating together on a limited edition product collection. The first of two collaborations celebrates Lucky In Love's 10th anniversary. K-Swiss used the pickleball industry's best-selling footwear model, Hypercourt Express 2, and worked with Lucky In Love founder Brad Singer and his design team to create a shoe that features the brand's anniversary messaging and more. The Hypercourt Express 2 x Lucky in Love shoe has features not available on any other current K-Swiss footwear model, including collaboration logos, iridescent heel, and shank.
Average Weight: 11.3 oz. (Size 8.5)
Color: Grey/Orange/Multi


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Delasha Long
Gather Platform | Chicago, IL
Delasha Long is a graduate of the Masters Program at the Department of Writing, Rhetoric, and Discourse at DePaul University. Her background is in content strategy, design, production, and management. She got her start in literary publishing, and hopes to use her talents in this field as well. In addition to her work with the Goldin Institute, Delasha works as the standing Journal Manager and upcoming Assistant Production and Design Manager for the Community Literacy Journal. Through a partnership with DePaul University, Delasha provides content strategy and research for the Gather Platform, currently assisting with the UX design, user testing and technical writing.