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NFT Marketplace For Sports
Inspiring your sports NFT marketplace with a generally acknowledged crowd for better market perceivability. The showy idea of sports acquires a solid foundation with its novel collectibles.
Enhancing Sports NFTs
Blockchain - a cutting edge innovation influences the development of the whole society across the globe. Its huge highlights and advantages support the modernization of the climate through innovation in flexible businesses. Its radiant origin of NFT in the crypto biological system assumes a significant part in offering speculation openings in the advanced medium. The primary NFT standard was presented by the pioneers of token turn of events, Ethereum. The NFT principles go about as a computerized portrayal of one of a kind collectibles in a decentralized medium, which adds inherent incentive for your exceptional resources and quick liquidity.
The plentiful administrations offered by NFTs draws in a gigantic crowd towards the interesting stage to purchase, sell and bid for the novel collectibles. The gigantic foothold towards the stage pulls in financial backers and business engineers towards the improvement of NFT for its ideal prizes. The worth of NFT may spike to millions contingent upon the shortage and crowd interest. The non-fungible tokens' one of a kind sort offers the chance for makers, specialists, artists, and social figures to make their work into NFTs for colossal advantages.
While the remarkable fascination looking for the stage advances different businesses towards a superior income stream, sports isn't a special case for controlling modern innovation to receive the rewards of a decentralized climate. Foster your games at the NFT commercial center at this point!
How does NFT contribute to the sports industry?
Sports is a supreme factor that treasures the crowd with its publicity and goosebumps second in the field. The adrenaline surge speeds up in our blood through the visual experience. The experience and the surge from the games pull in individuals towards it from the day we wander.
Bringing back cherished recollections of gathering sports cards, banners, memorabilia as NFT sports collectables draws in an adaptable crowd towards the stage paying little mind to age. The blend of two attractions looking for stages like games and NFT draws in immense rush hour gridlock towards the stage and proffers a superior income stream.
Sports collectables like player cards, pictures, video clips, adornments and different collectables can be made as NFTs that pull in an immense crowd towards the stage. The fascination towards sports and the tremendous fan base of select players are unique. Making players pictures, recordings, signatures and frill as NFT brings an immense deal for your NFTs. Brands addressing selective players and dispatching their item with their portrayal is one of the item boosting factors and a promoting procedure for business reevaluation. Building a remarkable NFT stage only for sports offers more noteworthy market perceivability and pulls in a colossal group towards your foundation.
Tokenizable assets in Our Sports NFT Marketplace
Exchanging Cards
Exchanging cards are the tremendous traffic generators towards the stage which draws in gigantic groups for the novel assortment of player's cards and pictures. Gathering the customary exchanging cards the type of NFT keeps your assortment exceptional and furthermore expands your resource esteem in the commercial center, which is likewise enjoyable to possess a special assortment of NFT cards.
Players' adornments like shirts, shoes, wristbands, covers, coolers get an immense greeting from the crowd. These are a remarkable collectable that deals with a steady increment of its worth, all things considered. Changing over it as NFTs offers immense income and possession rights for your resources with eminence.
Memorabilia like honors, prizes, signatures and athletic gear of selective players are broadly acknowledged and in-requesting special resources in sell off houses.Tokenizing a particularly collectable into NFTs offers generous advantages and incredible fortunes for your NFTs.
Video cuts
Tokenizing Exclusive Video clips and photos of players into NFTs acquires enormous footing to the stage. Hardly any early adopters have dispatched their commercial center for a specific game and encountering colossal achievement in the pattern of NFTs.
Benefits of Our sports NFT marketplace
- Increases the value of your remarkable collectible.
- Works with players to make their pictures and marks as NFT.
- Assemble significant advantages and prizes for your NFTs.
- Offers quick liquidity. Works with a steady venture and an open door in computerized resources.
- Offers sovereignty for the players for their interesting NFTs.
- The rage for sports draws in a colossal group for NFTs Elevates your market perceivability Brand promoting gets productive by the commitment of players. Empowers portrayal of select players as NFT in games.
NFT platform for Fantasy sports
Dream sports are the special games offered in the virtual medium. The stage works with the clients to choose players and structure groups to play for the booked matches. The players and the groups addressed as NFTs on the stage. Clients can choose to buy players and groups as NFTs to play in the booked matches. The triumphant group procures its awards as NFTs with extra advantages. It works with the player to get the sovereignty for the computerized portrayal of their characters. These NFTs offer an elite gaming experience with reasonable characters in the virtual climate.
Characteristics of Our NFT Marketplace for Sports
NFTs exceptional highlights and detail of interesting resources makes the token non-interchangeable, which implies one NFT can't be supplanted with another NFT because of its remarkable nature.
NFTs novel worth and the portrayal of exceptional resources makes the token in-separable in nature, which implies the tokens can't be moved in allotments.
NFTs are at their top because of their interest and shortage. Indeed, even the engineers are confined to make restricted measures of NFTs in similar kind to keep up its shortage.
Numerous crypto aficionados have presented one of a kind guidelines for NFTs in different blockchains for expanded execution and throughput.
NFT guidelines offer interoperability by posting the task and permitting to buy NFTs in various commercial centers.
Computerized posting
NFT commercial center works with computerized posting across numerous commercial centers once the venture is made.
NFT Token standards
Ethereum token standard ERC - 721 works with the advanced portrayal of your extraordinary resources. These NFTs have novel highlights and attributes which enhance your one of a kind resources.
Enjin's 1155 offers semi fungibility for your NFTs that permits the client to exchange their NFTs. This symbolic standard offers adaptability and versatility for your special resources.
This token contains various ERC-721 or ERC20. The ERC-998 standard permits making numerous tokens in the very chain of importance that empowers the clients to possess different NFTs or different tokens that has a place with a specific NFT in a solitary buy
TRC-721 symbolic standard is delivered by TRON, a huge blockchain network. This symbolic standard is the legacy of ERC-721 guidelines from ethereum that acquires its center highlights like straightforwardness, adaptability and security and offers better throughput.
EIP-2309 is an expansion of ERC - 721 guidelines that permits the back to back execution of exchanges consequently. This works with the creation and exchange of various NFTs in a consecutive request. By this expansion ,the client can ready to make and move a large number of NFT in a solitary execution.
Current Sports NFT Marketplaces
Barely any early adopters of NFT significant the business opportunity in sports and dispatched their remarkable NFT commercial center games to receive its rewards.
NBA Top Shots
Neat labs dispatched a commercial center called NBA Top Shots in Matic blockchain which solely offers the video clips and exchanging cards of NBA players and their uncommon minutes. This made a colossal rage among the crowd to buy the NFT from their novel commercial center.
Sorare is a novel commercial center for fantasy sports that picks soccer as their specialty to help their income and offers a virtual gaming experience in a dream association where the clients can purchase players and groups as NFTs to partake in the booked classes. The triumphant group acquires the prizes as NFTs. This gives immense market perceivability in the crypto swarm who endeavor to take an interest and receive the rewards.
Launch your NFT Marketplace for Sports with NFT Development Company
Osiz being a top-rated NFT Development Company that provides NFT Marketplace Development for Sports with premium features and functionalities that aids the user with hassle-free NFT trading for their passionate fantasy sports over a secured blockchain platform. Osiz has a pool of NFT experts having groundbreaking knowledge in building NFT Marketplaces.
Top Reasons Choose Osiz for NFT marketplace development for Sports
- Futuristic technology
- Reliable platform development
- Multi-layer security
- Transparency
- Cross-chain integration
- Innovative solution
Create your NFT Marketplace for Sports with Osiz technologies, Get a Free Consultation from our NFT Experts!
Call/Whatsapp: +91 9442164852
Telegram: Osiz_Tech
Skype: Osiz.tech
Email: firstname.lastname@example.com
Blockchain is a tamper-proof, distributed database that could change the way many industries operate. If you are interested in learning how to develop...Read More...
In an era defined by rapid technological advancement, the concept of the Metaverse emerges as a groundbreaking frontier. It represents a virtual unive...Read More...
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subset: commoncrawl


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Data can be stolen from an air gapped personal computer just by using variations in screen brightness. Researchers at Ben-Gurion University wrote a paper on it.
As the team defines them, "Air-gapped computers are systems that are kept isolated from the Internet since they store or process sensitive information."
That they have come up with yet another discovery on how to wrest sensitive data from a computer came as no shock to Naked Security, which recognized that "Researchers at Ben-Gurion University of the Negev have made a name for themselves figuring out how to get data out of air-gapped computers. They've dreamed up ways to communicate using speakers, blinking LEDs in PCs, infrared lights in surveillance cameras, and even computer fans."


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Date: 2018-07-12 14:40:03
Dog: Yay, It's time to meet my friends. It's the best time of the day for my tummy.
Dog: It's just my way of showing love in the morning. And also, another reason is, I don't want you to get scolding from mom for waking late.
Dog: Finally... I am getting my reward for being a cute obedient dog... hain na hain na hain na, pat on my shoulder...yay!!
Dog: Here comes my gang, come, sit anywhere you want but not on the sofa, we purchased it a short while ago. If you want some water, you can have it from my bowl - let's bond.
Dog: I am the boss of this entire locality, who the fuck did it on my place.
Dog: I thought we were friends!! This is how you treat your loyal friend? And what's this porcupine style you are doing with my hair, it's too old fashion!!
Dog: It's party, It's party....Oh my holy god!! No I am not going, leave me alone. I have lost my faith in you.
Dog: No, it's not me, I was catching the cat - she entered without our permission. Ok sorry... I wanted that cream biscuit. So can I get now??
Dog: I hope mom made something not so regular this time, I think she has made egg curry for me...only for me - I am not sharing with anyone. Thank You Maa, I will never break your glasses again!!
Dog: That feels like I am in heaven...I wish if you could get it all through my life.
Dog: I know you are upset I can feel that. Give me the name of that ass who made you sad, I will bite his ass hard as ever.
You know what I can't see you sad, so please smile...Okay, I am not going to scare your girlfriend next time... Now smile.
Alba Flores - Nairobi, Age, Contact No, Boyfriend, Net Worth, Family And More.
2022-11-15 12:41:28.0
Mrunal Thakur - Contact No, Age, Biography, Boyfriend, Family, Net Worth, And More.
2022-11-12 11:42:45.0
Naga Chaitanya: Wife, Age, Movies, Net Worth, Cars, Address, And More Details Inside.
2022-09-13 11:14:28.0
©To Clap2Ram Media (TabloidXO™)
Phunsukh Wangdu Aka Sonam Wangchuk To Help Indian Army Build Solar Huts To Keep Jawans Warm In Icy Weather
Gursoach Kaur, The First Sikh Turbaned Female Officer Who Joined The New York Police Dept, 'Chak De Phatte'
After Watching Deadpool And Spiderman Dance On Ranveer Singh Khalibali Song, You Will Shake Your Legs Too
Few Minutes To Go For Sonam Kapoor And Anand Ahuja To Get Married, Here Are The Images From The Venue
Introducing Peachpreet Khamba, The Punjabi Doggy On Instagram Who Can Talk Punjabi & Who Can Walk Punjabi


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Fees and fine print Compare plans
Starry Internet has recently emerged as a competitor to Xfinity in major markets across the US, with more major cities like San Francisco prepping to launch service in the mid-2020s.
While Starry has the best overall pricing for most users — particularly renters — there are some instances where Xfinity actually makes more sense in terms of price.
Starry is the best pick for cord cutters, thanks to the lack of data caps and low internet-only pricing.
Xfinity vs Starry Internet Basic Features
|Comcast Xfinity
|Starry Internet
|Starting price
|$19.99/mo. sign-up price
|$50/mo. flat rate
|Download speed range
|75–1,200 Mbps
|200 Mbps
|Upload speed range
|5–35 Mbps
|200 Mbps
|Network type
|Cable, Fiber
|Fixed Wireless
|Contract terms
|Contract required for cheapest price
|Current Deals
|View Xfinity Deals
|View Starry Deals
View Xfinity Internet Plans → View Starry Plans →
Xfinity vs Starry Data Usage Policies
Many internet providers in California use data caps to manage their network, meaning that if you use more than a certain amount of data, you will be charged extra on top of your monthly bill.
Xfinity has data caps on some plans. 1.2TB is a common cap on Xfinity internet plans. Starry offers unlimited plans with no data caps.
Xfinity places data caps on some plans.
Starry offers internet plans with unlimited data
Xfinity vs Starry Net Neutrality Policies
Comcast has a public statement of support for Net Neutrality on their corporate website, but have removed some promises on the page related to paid prioritization of traffic in recent years.
Starry Internet maintains a detailed page on their website describing their traffic treatment in regards to Net Neutrality.
Xfinity vs Starry Pricing and Fees
Here is a breakdown of how common add-on fees compare for Xfinity and Starry plans. These types of fees are common with internet providers, and can result in larger bill each month.
|WiFi equipment fee
|Price increases
|Bill increases after promo period
|Flat rate
|Early Termination Fee
|Fee to cancel contract
|Fee to cancel contract
Note that fee amounts described above may vary by plan. See the plan comparison tables for Xfinity and Starry below for more information.
Xfinity vs Starry Internet-Only Plan Comparison
|Most Common Plan
|Internet speed
|Sign-up price
|Final price
|Avg price over 2 yrs
|Fast (Xfinity)
|400 Mbps
|Order Online
|Starry Internet (Starry Internet)
|200 Mbps
|Order Online
View Xfinity Internet Plans → View Starry Plans →
The price of the Xfinity Fast internet plan is $55/month for the first Contract-Free. However, the price then rises 33% to a final price of $73.
So, that means the price you actually pay for internet, averaged over two years, is $64/month.
Remember that Fast includes the following fees when you compare pricing:
- $0/month fee for WiFi equipment
- Fees for using more than 1.2 TB
Starry Internet uses flat pricing for their entry-level Starry Internet plan. The $50 monthly cost does not rise over time.
Choosing between Xfinity and Starry plans
Xfinity's extensive cable TV bundle options are the primary draw for their service. If you have a choice between Starry and Xfinity, and you prefer Netflix to traditional cable, Starry will almost certainly be the best value in terms of monthly price.
As a new company, Starry is also particularly focused on customer service, an area where Xfinity's reputation is less strong.
Xfinity Internet Plans
|Plan Name
|Download Speed
|Promo Price
|Final Price
|75 Mbps
|Connect More
|200 Mbps
|400 Mbps
|800 Mbps
|1000 Mbps
|Gigabit Extra
|1200 Mbps
Starry Internet Plans
|Plan Name
|Download Speed
|Final Price
|Starry Internet
|200 Mbps
Frequently Asked Questions
Is Starry Internet cheaper than Comcast Xfinity?
Starry internet charges a flat $50 per month for 200 Mbps download and upload internet service. Xfinity has plans that start at a lower rate, but their plans have timed pricing, meaning that your rate will go up in your second and third year of service. Additionally, Xfinity has a maximum of 35 Mbps upload in the vast majority of their coverage area.
Is Starry Internet good for working from home?
Starry Internet has 200 Mbps upload speeds, making it a good choice for working from home since the bandwidth can easily support upload-intensive applications like Skype, and quickly move files to and from the cloud.
Is Starry Internet faster than Xfinity?
Starry Internet has faster uploads than Xfinity, with a standard 200 Mbps upload rate. Xfinity caps out at 35 Mbps upload, unless you can pay $300+ per month for their two-gig plan. Starry does not offer download speeds above 200 Mbps, while Xfinity offers download speeds up to 940 Mbps or more in most of their service area.


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Key Companies Covered in Distributed Antenna System Market Research Report are JMA Wireless, Corning Incorporated, Cobham Wireless, SOLiD, ADRF (Advanced RF Technologies, Inc.), Comba Telecom, Tesco, Dali Wireless, Boingo Wireless
Pune, Sept. 16, 2020 (GLOBE NEWSWIRE) — The increasing mobile data traffic all around the world is creating growth opportunities for the global distributed antenna system market, finds Fortune Business Insights in their new study. The study is titled, "Distributed Antenna System Market Size, Share and Global Trend by Components (Hardware & Service), By Technology (Cellular/Commercial DAS & Public Safety DAS), By Applications (Hospitality, Education, Transportation and others) and Geography Forecast till 2025."
According to Fortune Business Insights, cellular or commercial DAS systems segment is expected to dominate the market in terms of technology. This is because such devices have multifunctional terminals that offer a range of applications making better signal receptions more relevant and that too on a single platform
Click here to get the short-term and long-term impact of COVID-19 on this market.
Please visit: https://www.fortunebusinessinsights.com/industry-reports/distributed-antenna-system-market-100328
What Does the Report Offer?
- Valuable insights into the upcoming market opportunities;
- Tangible analysis of the market drivers, segments, and restraints;
- Exhaustive examination of the key market players and their strategies; and
- Microscopic study of the regional dynamics influencing the market.
Complications associated with upgradation of distributed antenna system technology and high installation cost accompanying DAS are expected to restrain the global distributed antenna system market to a certain extent. However, the rising need to boost cellular signals, surging mobile data traffic, increasing investments by telecom companies, and growing DAS and fiber popularity are anticipated to increase the demand in the global distributed antenna system market. Additionally, increasing use of smartphones, rapid urbanization, and rising demand for higher bandwidth for communication applications are forecast to enable growth in the global DAS market over the forecast period.
Have any Query? Speak to Analyst at: https://www.fortunebusinessinsights.com/enquiry/speak-to-analyst/distributed-antenna-system-market-100328
Top Players List:
- JMA Wireless
- Corning Incorporated
- Cobham Wireless
- SOLiD
- ADRF (Advanced RF Technologies, Inc.)
- Comba Telecom
- Tesco
- Dali Wireless
- Boingo Wireless
Key Market Driver –
- Surging mobile data traffic and increasing smartphone and internet penetration.
- growing popularity of fiber & digital DAS coupled with increasing investments by telecom companies in DAS.
Key Market Restraint –
- Difficulty in upgradation of distributed antenna systems when a new technology is available.
Request a Sample Copy of the Global Market Research Report: https://www.fortunebusinessinsights.com/enquiry/request-sample-pdf/distributed-antenna-system-market-100328
- By Components
- By Technology
- Cellular/Commercial DAS
- Public Safety DAS
- By Applications
- Hospitality
- Education
- Transportation
- Government
- IT & Telecom
- Others (Institutions)
- By Geography
- North America (USA and Canada)
- Europe (UK, Germany, France, Italy, Spain, Scandinavia and Rest of Europe)
- Asia Pacific (Japan, China, India, Australia, Southeast Asia and Rest of Asia Pacific)
- Latin America (Brazil, Mexico and Rest of Latin America)
- Middle East & Africa (Sosuth Africa, GCC and Rest of Middle East & Africa)
"Awareness initiatives adopted by government across undeveloped nations, aimed at educating people about the benefits of the internet has fueled the demand for smartphones and good speed internet, subsequently enabling growth in the distributed antenna system market," said a lead analyst at Fortune Business Insights.
North America holds the highest share in the global distributed antenna system market in terms of revenue and is expected to remain at the top through the forecast period. The presence of several leading players of the market has become growth engines to the market in North America.
Request for Customization: https://www.fortunebusinessinsights.com/enquiry/customization/distributed-antenna-system-market-100328
Adoption of 4G and 5G Networks to Boost Market
Various factors are responsible for the growth of the global distribution antenna system market. One of the key factors is the rising popularity of wireless data and their increasing demand. This, coupled with the rising adoption of smartphones, is driving the global market. The deployment of newer networks such as 4G and 5G and increasing affordability of people worldwide, will create lucrative growth opportunities for the market. Furthermore, the fact that nowadays the switching cost of one service provider to another is low, mobile network operators are compelled to provide quality in their network. Thus, uninterrupted connectivity and extended network coverage will boost the market during the forecast period.
The rising demand for Internet of Things (IoT) and increasing need for spectrum efficiency are key factors propelling growth of public safety distribution antenna system market. The demand for public safety connectivity will help the market gain momentum in the coming years. In addition to this, increasing consumer inclination towards online platforms for various activities such as shopping, banking, and others will drive the market.
However, the market may face challenges in terms of high cost associated with the deployment of distributed antenna systems. Coupled with this, complexities associated with the installation of distributed antenna systems and stringent government regulations relating to cost of connectivity are likely to restrict growth of the market.
Nevertheless, the increasing demand for public safety connectivity and increasing mobile data traffic will help the market grow during the forecast period. The rising number of malls, metros, stadiums, high-storied buildings, and other public places is also adding boost to the global market for distribution antenna system market as these public places demand installation of internet connection and thus, need super quality systems and broader coverage.
A Majority of Market Players Are Striving for Improving their Quality of Services
In terms of geography, Fortune Business Insights expects North America to hold the largest share in the market in terms of revenue. It is likely to remain dominant through the forecast period because of the strong firm base and majority of market vendors in the region such as American Towers Corporation IP LLC, and Corning Incorporated. In Asia Pacific, the market is likely to gain from the rising demand from China and India. Furthermore, the increasing penetration of smartphones and wireless telecommunication technology will pave way for the market's growth in Asia Pacific.
Some of the players operating in the global distribution antenna system market are CommScope Holding Company Inc., Tesco, Boingo Wireless Inc., Cobham WirelessSOLiD Inc., Dali Wireless Inc., Corning Incorporated, Advanced RF Technologies Inc., Comba Telecom, JMA Wireless, and Systems Holdings Ltd.
Pre Book Distributed Antenna System Market Report @ https://www.fortunebusinessinsights.com/checkout-page/100328
Have a Look at Related Research Insights:
3D Printing Market Size, Share & Industry Analysis, By Component (Hardware, Software, and Services), By Technology (Fused Deposition Modeling, Selective Laser Sintering, Stereolithography, Direct Metal Laser Sintering, PolyJet, Multi Jet Fusion (MJF), Others) By Applications (Prototyping, Production, Proof of Concept, and Others), By End-Use (Automotive, Aerospace and Defense, Healthcare, Others) and Regional Forecast, 2019-2026
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One More Folded Sunset
(still quite warm)
Friday, August 31, 2012
First & 52nd, Scenes of the Apocalypse
This 1890s warehouse, its roof mostly sky, stands like some bomb-blasted church. It reaches most of the way down 52nd. At the bottom of the street: Utz trucks, water and the Sanitation salt piles.
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Friday, August 31, 2012
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Opuntia Genus
Cactus Family
Opuntias are the pricklly pear cacti. The plant is composed of flat pads. Glochids are present at the aeroles. Large spines are usually, but not always present. Classification at the species level is often rather difficult. The opuntias hybridize creating intergradations between species. For example, in the west foothills of the Sandia Mountains at 6,200 foot elevation Opuntia engelmanii, Opuntia polycantha, and Opuntia erinacea are common. Clear examples of each species abound. Examples of species with characteristics intermediate between erinacea and polycantha are also observed. Similiar descriptions are noted in many references.
Distinguishing between the different species of Opuntia can be difficult. To aid this process check the pages sorting opuntia (A-E), sorting Opuntia (E-R) and sorting opuntia (S-T). The most important single characteristic is location, then elevation. Next is the overall size of the plant. Remaining characteristics are those in the "sorting opuntia" pages. Note each characteristic before attempting an identification because many species hybridize. For more complete material see the "efloras" link below. Many of the characteristics herein are derived from efloras, Weber, or Harrington.
Generally the larger opuntias occur at the lower elevations in the warmer climes, for example in southern Arizona or around Big Bend, Texas. Smaller and medium sized opuntias tend to be widespread, for example fragilis and polycantha which occur througout the rocky mountain region. The genetic differences between the Opuntia are confusing to this author. They may eventually lead to a better classification of the opuntia. Presentlly the species are separated by the observed differences noted in the "sorting opuntia" links above.
Beavertail Prickly Pear
Beavertail Prickly Pear, Opuntia basilaris (basal), growing at the Sonoran Desert Museum just west of Tucson, Arizona, and at Valley of Fires State Park, Utah.
Pancake Prickly Pear
Native of Sonoran and perhaps Mohave Deserts.
|Opuntia engelmanii, Engelmann's Prickly Pear is a robust prickly pear with relatively large pads. Pads are 6 to 8 in. (15 to 20 cm )acoss, aeroles are about 32 mm
apart, central spines 35 mm long with 1 central and 2-3 radial. Pad thickness is 15 mm. Occurs in California, Arizona, Nevada, Utah, New Mexico and fairly far east into Texas, but not in Colorado. A number of varieties of engelmanii occur. In general the pads are large, the plants are erect and large, and the spines range from sparse to moderately dense.
erinacea var hystricina
Porcupine Prickly Pear, Opuntia erinacea hystricina (Gr. hedgehog, porcupine), growing at the Sonoran Desert Museum, 2,560 Feet elevation, March 7, 2007. Very densely spined prickly pear with small pads, pad dia 75 mm.,Spines 1-2 central about 37mm long, aeroles 7mm apart, radial spines about 9, len up to 25mm. radial spines ranked in two sizes, pad width 23 mm. Erinacea pads are smaller (5-19 cm long, 3-11 cm wide ) than those of phaecantha (10-40 cm. long, 7-25 cm wide) and polycantha (5-15 cm long, 4-12 cm. wide). Numerous varieties occur including ursina, in which the spines are so dense that the pads cannot be observed. This variety is called Grizzly Bear Prickly Pear. Glochids in a caplike arrangement. Aeroles 8-14 per diagonal row across the pad. This flower is yellow with a greenish center but this is not generally distinctive. Note in some references erinacea is now considered a varity of polycantha.
Brown Spined Prickly Pear
|Distinctive characteristics: Pads smaller than engelmanii, larger than polycantha., glochids in cresent as above, tepals yellow often with red base, stamens red, pistils yellow.
Many Spined Prickly Pear
|Distinctive Characteristics: Pads smaller than phaecantha but larger than erinacea, distinctly wrinkled or bumpy, spines dense, but not as dense as erinacea, glochids in cresent with extra tuft as in figure above, flowers yellow to yellow green, with yellow filaments and pistils.


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Need More Information?
I'm Here To Assist You
Something isn't Clear?
Feel free to contact me, and I will be more than happy to answer all of your questions.
Feel free to contact me, and I will be more than happy to answer all of your questions.
Cancer Care is a Cooperative Job
Medical facilities and professionals engaged in cancer care may join us as a Histofi Partner Provider (HPP) in their respective geographic location.
Histofi Affiliated Pathology Laboratories: the affiliated pathology laboratories are designated as Histofi partner providers (HPP) and also scans glass slides of non-affiliated pathology and medical facilities in their own geography.
Non-affiliated Pathology Laboratories: sign up with the Histofi SaaS platform for your own account to optimize cancer-related services. Such non-affiliated labs need to ship pathology glass slides to the nearest HPP for digitalization.


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Eduardo Camavinga's agent cast a real doubt over Madrid acquiring his services.
Plenty of sources had tipped the Galacticos to be Camavinga's next destination.
The teenage sensation has gone from strength to strength in Ligue 1, becoming the youngest person to make his debut for France in over 100 years. His agent is Jonathan Barnett who also represents Gareth Bale. He does not share the best relationship with the Los Blancos hierarchy.
In quotes carried by Marca, Barnett said, "Real Madrid are going through very tough times now, maybe the Spanish teams cannot afford him.
"So unfortunately we have to wait and see what happens. From my perspective, Real Madrid is a great club, a great club to play for. Eduardo will be able to choose between many teams if Rennes decides to let him leave.
"It is not just a matter of one club, there are many clubs. We have to sit down and talk to Rennes and other clubs. It is a decision for Rennes because there is a contract involved.
"We are the agents, not his father. Before he had another agent and his father was only present; he has done positive things. He is a very sensible man and I have a lot of respect for him."
READ MORE: Top 5 best Real Madrid players of decade (2011-2020)


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B2B Marketing
that deliver
that deliver
So many B2B marketing solutions fail. They're inconsistent, they're delivered to the wrong person or at the wrong time, they fail to connect, they're boring, they're poorly designed – the reasons are all fairly obvious to outside observers. But to those working in the trenches every day to generate leads, provide information and close deals, it can be overwhelming to take a step back to recalibrate the machine while its running. This is where – and when – Neely & Daughters can provide calculable value through our suite of B2B marketing solutions.
Our services start with understanding your brand: where it's been and where you want to take it. From there, we craft the strategies, plans, content, collateral and delivery mechanisms necessary to tell your story, make it accessible, put it in front of buyers and make it all work in concert with your brand vision. Our services are available packaged together or a la carte to best fit your requirements and goals. We are also happy to provide the analysis necessary to prove that our solutions deliver ROI. It's what we do.
our services
our partners
From our founding, Neely & Daughters has taken pride in partnering with true visionaries.
The creative, strategic, and technological firms we trust with our clients' brands lead their
respective fields – and they're as fun, thoughtful, and reliable as we are.
Get to know them by clicking on the links below, or ask us for a direct introduction.
our process
We begin with intuitive listening: a process of passive engagement by which we hear your wants, needs, and desires and feel what is unspoken. This is also where we couple intuition with AI — using data science and analytics to further understand where you've been and what your audience is saying, thinking, and feeling.
This is when the picture truly begins to form. Using the physical and emotional information we've gathered, we passively allow the intel to take root and become pure epic vision. Once the idea has come into being, we develop a creative strategy and timeline that will allow for optimal execution.
Execution is all about bringing our creative vision to life. Strategy, content, design, and presentation are painstakingly worked in-house until the end product gets our official seal of approval. By the end of this stage, when we feel we've done the concept justice, the final deliverables will be presented.
This is when we drop the bomb. Our epic vision is released into the world, carefully orchestrated to fit into timelines, cycles, and the current climate for maximum impact. Launch is always a major celebration. It's time to sit back for a minute, bust out the popcorn, and enjoy the fireworks.
The analysis stage is when we take a hard look at the impact we're making. We gather new intel — this time in the form of media, coverage, and public response. We now have hard data that proves we've struck hearts and started a revolution.
We don't ever rest on our laurels; instead, we continue to look for ways to expand, enrich, and connect. We strive for bigger, better, greater. Always. Our work doesn't live in a vacuum, and it's in no way impotent. Expect it to take on and take over the world.


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A judge earlier this week gave permission to a Stanford attorney, Stephen Cochell, to dismiss the lawsuit.
However, the lawyer said in a court filing that his client's claims "will be preserved" because the criminal case remains open.
The suit filed in court in Houston, Texas alleged that the Securities and Exchange Commission (SEC) – which charged him with defrauding investors out of US$7 billion through the sale of fraudulent certificates in Stanford International Bank (SIB) in Antigua – and prosecutors acted illegally in going after him.
According to the suit, the government agents "undertook illegal tactics" to prosecute Stanford and "engaged in unfair, abusive law enforcement methods and tactics" that left him broke and unable to properly defend himself.
The trial of the alleged fraudster, which was scheduled for last month, has been postponed indefinitely by US District Judge David Hittner while Stanford gets treatment for his addiction to anti-depressants.
He's being treated in a federal prison hospital in Butner, North Carolina.
(Parts of this article were written with content incorporated from a Caribbean360 release)


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Nebulous curtains
fly apart, revealing an
eternal half-orb.
fly apart, revealing an
eternal half-orb.
The message is supreme;
Born in the heart,
and lilting itself
from tongue to tongue,
throwing its scent
over wind and wave;
travelling on dots
or fingers
when blindness
or silence bar its way.
It hews itself into stone
or burns itself onto magnetic discs;
it is the message that lives
and I exist
solely to pass it on.


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Contact us
Give us a call or drop by anytime, we endeavour to answer all enquiries within 24 hours on business days. We will be happy to answer your questions.
Contact Us
Whether you need marketing tips, sales advice or tech support, our friendly team are on hand to help! Get in touch with us using one of the ways below:


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- Dance Book
- Where to dance
- Supplies
- About
The best spots around to go dancing on a FRIDAY. If your looking for lessons see the "learn" tab under "Where to dance"
16 Cooper St
Macksville, NSW 2447
Barry Noble 50/50 dance. Old time, New Vogue, novelty Macksville RSLRead morePinpoint
33-35 Hall St
Peak Crossing, QLD
Cnr Boonah Rd Peak Crossing Held on the 3rd & 5thRead morePinpoint
Cnr Pine Mountain Road and Quarry Road
Pine Mountain, QLD
Hall 1st Fri (except July & Jan)Pinpoint
Wellington St
Townsville, QLD 4810
Aitkenvale PCYC Variety Dancing Circle Inc Excellent Sound SystemPinpoint
Cnr Victoria Ave & Hornibrook Esp
Woody Point, QLD
Bramble Bay Bowls Club BandPinpoint


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Charismatic Fine Photography and Films is one of the most up-coming high-end photography and film companies in Las Vegas, NV. It has an online professional photographer portfolio, with several photos for our clients to look through for their viewing pleasure. DeLouis Enzo White, CEO and President of Charismatic Fine Photography, has been serving the Las Vegas, NV area for over 9 years. His strong passion for photography and films developed after he enrolled in the International Academy of Design & Technology. Enzo is labeled as one of the most dedicated and professional upcoming photographers today. His high quality services through his charismatic approach create a mesmerizing effect on the eye of the beholder. He enjoys shooting at luxury locations such as Town Square, Lake Las Vegas, City Center (Las Vegas, NV), Beverly Center(Los Angeles), Malibu and Santa Monica, CA. In the years Enzo has been in photography. His skills have afforded him the opportunity to work with several upcoming models and actresses who have since been seen in films, make-up ads and on magazines,he seeks to promote and enhance the skills of Models all over the World
International Academy


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Scytl's election management subsidiary, SOE Software, has been awarded a contract through a nationwide competitive solicitation by the Georgia Secretary of State's office to use Clarity Election Night Reporting (ENR) to present State and Federal election data from all 159 counties. Georgia joins 13 other statewide implementations that leverage the industry leading election results presentation solution. Clarity ENR is the nation's leading software presentation tool for elections with its graphical presentation of maps, bar charts, totals and downloadable reports. Georgia residents along with media and political campaigns will now be able to find detailed, precinct-specific election result data on election night from the Georgia Secretary of State's website. With this innovation, the State of Georgia and all its 159 counties can expect to reduce phone calls and data requests from the public while increasing openness and transparency to the public.
More news for you


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The Prince Eugen Medal (Swedish: Prins Eugen-medaljen) is a medal awarded by the King of Sweden for "outstanding artistic achievement."
The medal was established in 1945 by the then King of Sweden, Gustaf V, on the occasion of the eightieth anniversary of his brother, Prince Eugene, who was a well-known painter and art collector.
It is awarded every year on 5 November, the day of Eugene's name. It is presented to the winners at the Royal Palace in Stockholm.
Wikipedia contributors. (2021, January 27). Prince Eugen Medal. In Wikipedia, The Free Encyclopedia. Retrieved 22:42, February 16, 2021, from https://en.wikipedia.org/w/index.php?title=Prince_Eugen_Medal&oldid=1003181945
More on Design Awards and Competitions
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Marco Zanuso Sr. Italian architect and designer – Encyclopedia of Design
Marco Zanuso Sr. was an Italian architect and designer. He was born in Milan. He studied architecture at the Politecnico di Milano, to 1939. After World War II, he practised as a member of the Italian Modern movement. From 1945-86, was a professor of architecture, design, and town planning, Politecnico di Milano.
The Exposition Internationale des Arts et Techniques dans la Vie Moderne (International Exposition of Art and Technology in Modern Life) 1937 – Encyclopedia of Design
From 25 May to 25 November 1937, the Exposition Internationale des Arts et Techniques dans la Vie Moderne (International Art and Technology Exhibition in Modern Life) was held in Paris, France.


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MSc-level course at Vrije Universiteit Amsterdam, computer science
6 credits
Academic year 2018–2019, period 2
This courses introduces the proof assistant Lean, its type-theoretic foundations, and its applications to mathematics and computer science.
A proof assistant is used to check the correctness of a specification of a program or the proof of a mathematical theorem. The course is concerned with the proof assistant Lean, a system that is developed primarily at Microsoft Research. In the practical work, we learn to use Lean. We will see how to use the system to prove mathematical theorems in a precise, formal way, and how to verify small functional programs (e.g., a sorting algorithm). In the course, we focus on its dependent type theory and on the Curry–Howard–De Bruijn correspondence between proofs and functional programs (λ-terms). These concepts are the basis of Lean but also of other popular systems, including Agda, Coq, and Matita.
The course is oriented towards the application of proof assistants to computer science and mathematics. We will use Lean, a modern system developed at Microsoft Research. In each lecture, we will review a Lean file; for example, for the first lecture the file is called 11_lecture.lean. It can be downloaded from the materials section below.
If you need to contact us, please email both lecturers (J.C. Blanchette and J. Hölzl), with [LV] in the subject line. We will try to monitor Canvas regularly. You can also use Canvas for discussions and questions related to Lean.
Although the course is application-driven, this is a university course, so theory is important as well. We will cover it as it emerges, but you are expected to read the materials we refer to, such as Theorem Proving in Lean.
To each lecture (on Mondays and Thursdays) corresponds some notes in Markdown format (e.g., 11_notes.md), a Lean exercise sheet (e.g., 11_exercise_sheet.lean) and a Lean homework sheet (e.g., 11_homework_sheet.lean). The exercises are crucial; theorem proving can only be learned by doing. We will assist you during the group exercises (on Tuesdays and Fridays) and answer questions on Canvas. We will also help you set up Lean and Visual Studio code on your computer. See below for links to software and installation instructions.
For the group exercises, we strongly recommend that you bring your own wine^H^H^H^Hlaptop. Otherwise, you can also use the online Lean interface.
The homework given at lecture n is due by the beginning of lecture n + 2. The homework is mandatory for obtaining a passing grade at the end.
We have experience teaching Isabelle/HOL and Coq, but this is the first time we teach Lean. We are open to suggestions on how to enhance the teaching materials for future years. Of course, we will be happy to help if you run into difficulties. Please do not hesitate to contact us.
Exceptionally, the homework for lectures 3.3 and 4.1 will be replaced by a project. You can think up a nice formalization project for yourself, such as these:
You should set yourself a time limit before starting your project. Also incomplete/unfinished formalizations are welcome and will be graded. Please comment your formalization well, such that we can see what it does or is intended to do. You are welcome to discuss your plans with your instructors before starting your project.
The course will consist of 14 lectures:
The above list is subject to changes.
This year's course is the first of its kind, with the emphasis on practical applications and on dependent type theory in Lean. Below you will find a mock exam as well as some exams from previous years for related courses at the VU and the Universität des Saarlandes in Saarbrücken. These give a rough idea of what "formal proofs on paper" can look like.
Please note that the previous Logical Verification course had a stronger focus on the metatheory of dependent type theory and on the Curry–Howard correspondence (and less on practical applications), and the course in Saarbrücken covered Isabelle/HOL and language semantics, going well beyond what we achieve in part 3 of the current course. Closer to the exam, we will make some dedicated practice material available.
To participate at the exam, you must have submitted and passed all of the practical work.


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{originally written in March of 2015, two months before I remarried}
Let me say this upfront: I like Tall-Shadow. I love Tall-Shadow. I'm in love with Tall-Shadow. And I believe to my core that he likes, loves and is in love with me right back.
I mean, c'mon…
Let me also say upfront: I believe God is in this.
So when I say I'm scared to get married again, I do not mean I am scared to get married to my sweet man.
I think when I say I'm scared I mean a few things.
One, that all of my quirks that he thinks are now adorable will turn into annoyances and he will stop liking me and stop thinking I'm cute. You know, things like how I hate waking up to a dishwasher full of clean dishes. (I'd rather stay up late and unload or not start a full dishwasher til the morning. I have no idea why.) Or like how I have a fifty-seven step (okay, maybe eight-step) morning routine. And how I get a little, umm, something, when it's disrupted. Or like how I really, really prefer getting ready by myself instead of sharing the bathroom. Or…I can go on. Trust me, I can go on.
Two, that all of my quirks that I have gotten used to will be impeded upon, and I will become not so nice anymore. You know, because I'm high maintenance. And I've, at this point, been living as a single person for four-and-a-half-years. And because I'm old (-ish). And because I'm set in my ways. And because I'm way less go-with-the-flow than he gives me credit for. And because I'm stubborn. And because I can be super selfish.
But those are kinda little things. So I think even more than all that, are these bigger things.
Three, that the other shoe will drop, because the part of me that has seen so much relational pain believes deep down that the other shoe always, always drops.
And what will happen if we get married and I wake up and…
…he stops loving me? (Though I've never been more consistently loved in my life.)
…he's mean to me? (Though he's never been mean to me.)
…an addiction crops up? (Though if he had one, I'd know by now.)
…we start fighting all the time? (Though he and I get along really, really, really well.)
(I want to be clear: these fears are fears I would have with any future husband of mine; it's not as if I've seen red flags with Tall-Shadow that I am pretending aren't there. Not at all.)
But lastly and possibly most significantlly, I am doubting my ability to be a good wife. Or, more to the point, to be a wife period. I was called some horrible things over the years: liar, moron, out of my mind, and idiot was implied on a regular basis. Hearing those words once would sting. Hearing those words for years changes your self-perception and self-worth. Well, not just changes it, makes it disappear. I saw myself through someone else's lens. And though I have done some major healing work these past three or four years, the thought of remarrying has woken up my demons and they are whispering to me again.
You don't have what it takes to be married.
You're going to fail him.
You're going to hurt him.
It won't last (because of you).
Marriage is hard for the average girl; but you are beyond deeply flawed.
You're an ass, remember?
You make life a living hell, remember?
But these fears are the fears of a girl whose heart has been deeply wounded, broken into a million pieces, discarded, not protected, lied to, not fought for, abused, manipulated.
These fears are the fears of a girl who is overlaying her past – unfairly – onto her present and future.
These fears are the fears of a girl who is – in tiny moments – forgetting that she's come a long way, forgetting that she's been brought deep healing to counteract the deep wounding, forgetting that the man who stands in front of her today is a new man to her, with baggage, yes, (just as I have baggage) but not hurtful baggage between us.
Here's the thing. I'd be a fool not to have these kinds of thoughts. I'm not a twenty-two-year old with no experience being married. I know what a hard marriage looks like. And I believe that God gave us the sweet gift of memory so that we can avoid making the same mistakes over and over again. And so therefore, I should be cautious and mindful as I approach my second marriage. It would be unwise of me not to be.
And yet, I am marrying a man who, and we have a relationship that, is as different as my first as night is from day. I am not replicating patterns. I did learn from my past and I have course-corrected.
But here's the other thing that I seem to be forgetting. I very well could wake up one day to all of those horrible things happening. (Just as sweet Tall-Shadow could wake up to me being not who he thought I was either, because we are both human.) But even if that's the case…then there's God. And I believe that even if everything isn't okay, that everything will EVENTUALLY be okay, and it will all be alright, and we will both get through.
So I'm not bracing myself for the worst, by any means. But I need to walk headlong into my future knowing that some bad may befall us. And yet I must choose to hold in my other hand, that if it does, we'll figure it out, and we'll be okay, and grace will carry us along.
Thinking about dating again? Or getting married again? Let's prep first!
If you are in a difficult marriage and you're wanting to learn how to stay in better and different ways, Marriage Methods is for you.
If you are in a difficult marriage and you're trying decide whether to stay or leave, Decision Time will bring clarity and steadiness.
If you are separated or divorced and you're in need of processing and healing, Heartbreak to Hope will move you forward.
Click on the links above to find out what comes in each course.
Then, if you're ready for some deep heart transformation (we start early September), click on this link.
You can pay the current valued price OR (for the first time ever) you can pay what you're able, and you can add on a coaching call or more if you need even more personalized coaching.
Register here by September 6.
Remember, sweet one, I have walked in your shoes.
I know the pain, the confusion, the sadness, the anxiety, the exhaustion.
And I have walked through to the other side.
And I have been helping women just like you walk through to the other side for ten years now.
You are not alone.
God is with you.
I am here for you.
You can and will get through this.
Much, much love,
xoxo Elisabeth


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It was predicted that Masataka Yoshida (30) of the Boston Red Sox would have an outstanding big league debut season.
Boston local media NESN said on the 13th (Korean time), "The prospect that Rafael Devers will be Boston's best player in 2023 is not surprising. But who the second best player will be is a matter of debate. The analysis of the fan graph, a baseball statistics site, provides a surprising prospect," he said, introducing Yoshida's expected performance this season.
Yoshida is a heavy hitter with a batting average of 302.7 (884 hits in 2703 at-bats), 133 homers, 467 RBIs, and an OPS of .960 in 762 Japanese professional baseball games. This year, he led the Orix championship by playing an active role in 119 games with a 바카라 batting average of 3.3 0.5 Lee (138 hits in 412 bats), 21 homers, 88 RBIs, and an OPS of 1.008. His strength is his sophisticated batting ability and pioneering vision. After the season, he announced his advance to the major leagues and signed a five-year, $90 million (approximately 112 billion won) contract with Boston. Immediately after his contract, there were many opinions in the local media that it was overpay.
Fangraphs is one of the best-known baseball statistics sites. According to Steamer, a performance prediction program provided by Fan Graph, Yoshida is expected to record a batting average of .298 (146 hits in 490 bats), 19 homers, 62 RBIs, and an OPS of .867 in 123 games this season. He plays as a major league player, and he has no shortage of results. Depending on the situation, it is worth aiming for the All-Star selection as well as the American League Rookie Award.
NESN said, "Devers was expected to record a WAR (contribution to victory over substitute) 4.5. Next is Yoshida (3.8). These predictions are really good reviews. Only 13 major league outfielders are expected to have a higher WAR than Yoshida."
NESN, who asked, "Are your expectations of Yoshida too high?" replied, "Maybe so. It's my first season in the United States and it seems a bit overkill to be grouped with top players. However, Boston paid about 15 million dollars (approximately 18.6 billion won) in posting fees to Orix, Yoshida's original team, and gave Yoshida 90 million dollars. Yoshida is a wild card worth 150 million dollars (approximately 130.6 billion won). If he gets the leadoff spot as expected and performs in line with FanGraph's predictions, Boston's bet will be worth it."


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The focus among traders today
will be on the European Central Bank (ECB), which will announce its interest
rates decision later today. The bank is expected to leave rates unchanged, with
the deposit facility rate at minus 0.4% and the marginal lending facility at
0.25%. The central bank is widely expected to lower the growth forecast for the
third time this year.
The bank has a number of key
concerns. First, the EU economy has strongly relied on exports for growth.
However, as the global trade gets interrupted by the war between United States
and China, there are concerns that this may affect the growth. Indeed, the
effects are already being felt. The economy grew at a four-year low of 0.2%
over the third and fourth quarter of 2018. This sluggish growth appears to be
continuing this year.
Second, the key countries of the
EU are having their own problems. Italy is in a recession. Germany, the key
engine of the economy avoided a recession narrowly in the fourth quarter. Spain
and France are going through political uncertainties, which could hinder
growth. Other smaller countries like Greece are not doing well either.
Third, the global economy is not
doing well either. Yesterday, OECD became the latest organization to lower the
global growth forecast. The organization said that the economy could grow by
3.3%, which might be lower than the previous forecast of 3.5%. Fourth, there is
a major risk of a no-deal Brexit, which will lead to more problems for the
While most traders expect the
bank to reduce the forecast in a big way, there are hopes that this may happen.
This is because the members of the ECB believe that the slowdown in the economy
is a soft patch and not an extended slowdown that has the potential to last
years. This confidence is because of the strength of the labor market, with the
unemployment rate at the lowest level in more than a decade. This has led to a
slight increase in household spending, which may mitigate the impacts of
smaller exports. Another reason is that increased government spending can help
to spur growth. Additionally, the bank intends to continue reinvesting 15
billion euros every month in the region's bond market as part of the
reinvestments of the 2.6 trillion QE program. These measures may help spur the
economy. Another option at the table is the Long-Term Refinancing Operations
(TLTROs), which could provide cheap financing to the region's banks.
Ahead of the ECB's decision, the
EUR/USD pair remains close to the lowest level since February. Yesterday, the
pair reached a low of 1.1285, with the pair being in consolidation mode. This
is confirmed by the narrow Bollinger Bands, while the RSI has remained at the
50s. The pair is looking like it could be very active today and tomorrow as
traders receive the ECB statement and US jobs numbers.
The post European Central Bank Interest Rates Decision Preview appeared first on Forex.Info.


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It's been a while since we had us some music from Mali.
Mamadi Diabate and Les Ambassadeurs are here to
fill in the gap. I am absolutely not the one to tell you
all about these musicians but I do know one thing,
this is a hell of a record and worth a couple of
spins. I'm sure it will make your sunday.
Don't forget, play and spread..
1 Moriba kaba
2 Yeke yeke
3 Iye iye
4 Talassa


text:
Another sports season, another round of "new stuff".
From fall into winter, football morphs into basketball and volleyball shifts into soccer. Speaking of soccer, the county's eight public programs are now in four new districts across three classes. Here's the new groupings:
District 6A-6: Osceola, Celebration, Cypress, Freedom, Haines City, Ridge
District 5A-9: St. Cloud, Poinciana, Liberty, Tohopekaliga, East River, New Smyrna Beach
District 5A-12: Harmony, South Fork, Sebastian River, Melbourne, Viera, Martin County
District 4A-5: Gateway, Deltona, Pine Ridge, Jones, Lake Minneola
Like in volleyball, teams aren't mandated to play each district foe in the regular season, as the district tournament seedings will be set by the MaxPreps computer rankings.
And like the volleyball Orange Belt Conference tournament, the county will do the same for its in-season event. In fact, the boys teams, at the agreement of its coaches, held its OBC tournament last week based on the rankings from last season. The result? Celebration, which had dominated county play the past three years, did not make it an OBC four-peat, losing to Harmony in penalty kicks in the semifinals after playing to a 2-2 draw. The Storm went on to take third place over St. Cloud, also in penalty kicks.
Penalty kicks were a theme of the OBC tournament. Osceola, who advanced to the championship game with a 5-3 win over St. Cloud thanks to a hat trick from Christian Aragon, played the Longhorns to PK after a 1-1 draw through regulation and overtime. Striker Cody Huff put Harmony up in the 10th minute, but Aragon tied it before halftime. Despite playing the match's final 17 minutes a man down, the Longhorns fought to the draw and goalkeeper Kevin Lavigne provided two saves in PKs as well as the game-winning goal to cap the victory.
Coach Brett Ballard's team looks to rebound from a 6-11 year. Huff and midfielders Matthew Espinosa, Carson Weis and Carlos Rodriguez and backs Bennett Forsberg and Gilbert Figueroa all return.
"This is a solid team with a lot of experience, we have built off of last year's runner up in OBC with only losing two seniors," Ballard said. "The versatility of the guys is our biggest strength and they can adapt to any system we face throughout the year. We are in a new district this year which puts us up against Brevard schools including powerhouse programs such as Melbourne and Viera. But if we want to be the best, we have to beat the best and the guys have responded well."
Celebration, which also has back-to-back state Final 4 appearances on the resume, and while the Storm have several big pairs of cleats to fill, they'll still be a force locally.
"It'll be hard to replicate what we have done in the past two seasons. Last year we scored 90 goals and 60 of them were from three of our seniors (Luis Mora, Matheus Machado, Fernando Abreu, all now logging play time as college freshmen)," Coach Chad Boudreaux said. "It was real easy for us to turn to one or a few players and let them take the game over. We are going to need the full collective to step up and play together to accomplish our goals this year. We had a preview of that when we played Harmony, in the last 15 minutes of the game you can see what we can do as a team if we play together."
Nick Romano, Gabe Pinheiro and Edward Galindo, important role players a year ago, will be the leaders up front. Newcomer Wilmer Garay has chipped in three goals in the Storm's first four games. Defensive stopper Oscar Newman returns, as go goalies Horacio Parisi and Jonathan Deer.
The Storm schedule is a Who's-Who: district foes Freedom and Cypress Creek, Lakewood Ranch, Lake Highland Prep and Vero Beach dot the lineup.
Tohopekaliga builds off a 6-12 season, but four of the wins came within the county. Now the Tigers are (finally) playing at home on campus.
And Coach Eddie Fernandez isn't shying away from anyone this year; his schedule also has Bishop Moore, Lake Mary, Lake Highland, Freedom, Lakewood Ranch and Montverde.
"I do believe in my team's capability and drive to win, going up against these schools," he said. "We have (new) kids, especially freshman, that have already impacted our varsity team like, Adrian Nunez, Gustavo Casanova, and Sophomore Sebastian Quijano."
But it's his seniors, like Carlos Jiminez, Jean Batista, and Will Johnson who will lead this team.
Expect St. Cloud to show some might this year, with scoring punch from Anderson Agudelo, Jack Baillie and Fabrizio Lucia. Senior goalkeeper Noah Craig is a force in the back. Osceola has Aragon and Ezequiel Rabelo to get the ball in the net,
Gateway, coached by Sagar Patel, has posted shutout wins over Liberty and Poinciana in the last week. The Chargers beat Gateway, 4-1, in an OBC tourney game to snag sixth place.
Girls soccer: Harmony, St. Cloud looking strong again
Harmony's been the dominant force in the OBC and their district the last three years, beating St. Cloud in either the district finals or semifinals, but now they're in different districts, so they can both win.
The girls will play their OBC tournament in mid-January.
Harmony girls should be competitive again, even after graduating 11 seniors. Jenna Butcofksi, who just signed with Florida Gulf Coast University, and sophomore Reinna Scott should do the heavy lifting for the offense while three-year starting goalie Angie Santos and sophomore Erin Jones will help anchor the defense. Newcomers Shayna Augusma (from Miami) and Victoria Suarez (New Jersey) could add punch to the offense.
With the new MaxPreps scheduling, the Longhorns are like other locally-competitive squads: the schedule might be the toughest one in Harmony history, with nine playoff teams from last year.
"Moving to a new district will also be tough as we move to a district with perennial powers Melbourne and Viera," HHS Coach Scott Marlega said.
St. Cloud Coach Shawn Beck said his team's dealing with some injuries, but the Bulldogs have opened the season 5-0 after tying Rockledge in the preseason.
The defense is solid with goalie Krystal Cruz-Torres and backs Madelyn Barthle and Nicole Longmore. Seniors Hanna Archibald and Ellie Schmidt, junior Zabrina Hellmer and sophomore Leilani Wei can control a midfield.
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The Bulldogs and Longhorns play at Harmony Dec. 13 in a likely preview of the OBC title match.


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Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS
Annals of Clinical Microbiology and Antimicrobials volume 17, Article number: 22 (2018)
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Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians.
We used 64 simulated blood cultures to establish the method of MALDI-TOF MS based ertapenem hydrolysis assay. The cutoff value of logRQ calculated from the peaks intensity of ertapenem and its hydrolysate was first set to identify the strains with carbapenemase activity. Then, we detected and calculated the logRQ values of 385 Enterobacteriaceae strains from positive clinical blood cultures to distinguish the carbapenemase producers and noncarbapenemase producers.
The mean logRQ value of 32 noncarbapenemase producers was − 0.85 ± 0.14 in simulated blood cultures, while the logRQ value of 32 carbapenemase producers was 0.87 ± 0.55. Thus, the cutoff value of logRQ was set at − 0.45 with sensitivity of 100% and specificity of 100%. In 385 clinical positive blood cultures, the logRQ values of all carbapenem-susceptible Enterobacteriaceae strains (81.3%, 313/385) were < − 0.45. Comparing with the detection of carbapenemase genes, carbapenem-resistant Enterobacteriaceae strains (18.7%, 72/385) were well distinguished by MALDI-TOF MS based ertapenem hydrolysis assay with a sensitivity of 92.5% and specificity of 100%.
Our data show that MALDI-TOF MS based ertapenem hydrolysis assay is a rapid and accurate method to detect carbapenemase activity of Enterobacteriaceae strains from positive blood cultures, and can be routinely performed in clinical microbiology laboratories.
Bloodstream infection is a severe threat for human life. As reported before, a mean survival rate of 7.6% was decreased if the effective treatment was applied per hour delay for patients in septic shock [1]. Therefore, early appropriate antibiotic treatment is important to improve prognosis of bloodstream infections [2]. Blood culture was considered as the 'gold standard' test for the detection of pathogens in blood specimens [3]. The rapid identification and susceptibility test reports for the pathogens in blood specimens are very meaningful for clinicians to choose appropriate and efficient antimicrobial agents for anti-infective therapy. Nowadays, Enterobacteriaceae strains are becoming the major pathogens which can cause bloodstream infections with an isolating rate up to 30% [4]. Carbapenem is the crucial antibacterial agent for primary empirical antibacterial therapy against infections caused by Enterobacteriaceae strains. However, In the past few years, along with the large-scale use of antibiotics, carbapenem resistance of Enterobacteriaceae strains has increased rapidly and become one of the most common global health issues [5], especially for the carbapenem resistance rate of clinical isolated Klebsiella pneumoniae, which has reached up to 10% in 2014 [6, 7]. In addition, empirical antibacterial monotherapy may not well inhibit the growth of resistant Enterobacteriaceae strains [8]. Therefore, it's important for clinical microbiology laboratories to give clinicians timely and believable antimicrobial reports for early anti-infective treatment.
MALDI-TOF MS has been routinely used to identify microorganisms in clinical microbiology laboratories. Recently, it's reported that MALDI-TOF MS has been applied to the antimicrobial resistance research, especially for the activity detection of hydrolytic enzyme that produced by microorganisms. Here, we evaluated the efficiency of MALDI-TOF MS for detection the carbapenemase hydrolyze activity of Enterobacteriaceae strains isolated from positive blood cultures.
Blood culture simulations
64 Enterobacteriaceae strains were collected from patient specimens in the clinical microbiology laboratory of Xinhua Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. The strains were constitute of 32 carbapenemase producers and 32 noncarbapenemase producers, and were stored in glycerol broth at − 80 °C. For each strain, after be inoculating and growing on Columbia blood plate at 35 °C, bacterial suspensions of 0.5 McFarland were prepared with 0.45% NaCl, and then diluted 100 times by 0.45% NaCl. 200 μL of bacterial suspensions were injected into the negative blood culture bottles to simulate blood culture specimens. These blood culture bottles were incubated at 35 °C in the BD BACTEC FX Blood Culture System (Bectom Dickinson), and were taken out and detected by MALDI-TOF MS based ertapenem hydrolysis assay when the positive alerting signal was observed from the system.
MALDI-TOF MS based ertapenem hydrolysis assay
4 mL samples from positive blood cultures were injected into serum separator tubes (ST740CG, INSEPACK®, Japan), and centrifuged at 3000 × g for 10 min in order to remove blood cells and debris at the bottom of tubes. The bacterial pellets at the surface of separation gel were carefully transferred into a 1.5 mL tube and washed twice using 500 μL 0.45% NaCl. Then the pellets were resuspended with 50 μL of ertapenem solution (0.2 mg mL−1 ertapenem, 20 mmol L−1 Tris–HCl, 0.45% NaCl) and then incubated at 35 °C for 2 h. The ertapenem solution without any bacteria was set as the blank control. After 2 h incubation, the suspensions were centrifuged at 3000 × g for 5 min. 1 μL of the supernatant was spotted on the target plate (Bruker Daltonics), subsequently mixed with 1 μL HCCA matrix solution (10 mg mL−1 cyano-4-hydroxycinnamic acid) and then dried at room temperature. Mass spectrum was acquired by FlexControl 3.0 software (Bruker Daltonics) of Microflex LT mass spectrometer (Bruker Daltonics) in the low mass-to-charge range (100–1000) with 60 Hz laser frequency and 200 laser shots. The matrix peaks (HCCA [M + H]+ at 190.05 and [2 M + H]+ at 379.02) were used for calibration per test. Further analysis of mass spectrum was performed by the Flexanalysis 3.0 (Bruker Daltonics). Firstly, the spectrum was smoothed and baseline was subtracted. Then, the specific ertapenem related peaks and hydrolysate related peaks were manually checked in the mass-to-charge range of 440–560.
LogRQ values
The logRQ values which were used to estimate the results of hydrolysis assay were calculated by the intensities of peaks. The formula was logRQ = log (sum of hydrolysis peak intensities)/(sum of ertapenem related peak intensities) [9, 10]. Since both of the peaks of ertapenem and its hydrolysed, decarboxylated form may be observed in the mass spectrums of ertapenem solutions incubated with carbapenemase or non-carbapenemase producers, a cutoff value of logRQ was necessary to distinguish these two groups. The cutoff value was acquired based on receiver operating characteristic curve (ROC) which was conducted to determine the accuracy of the logRQ values to estimate the results of hydrolysis assay.
Clinical blood cultures collection
Patient positive blood culture bottles which contained Enterobacteriaceae strains were collected from October 2015 to December 2016 in the clinical microbiology laboratory of Xinhua Hospital. Both of aerobic and anaerobic blood culture bottles (BD BACTEC, Bectom Dickinson) from different body parts of patients were included. After blood culture bottles were accepted at our laboratory, they were put in the BD BACTEC FX Blood Culture System (Bectom Dickinson) immediately until the positive signals were obtained. The blood cultures incubated in blood culture system for 5 days with no positive signals were considered as negative. The bacteria from blood cultures bottles with positive signals were collected under centrifugal force in serum separator tubes and were placed onto the target plate for directly identification by MALDI-TOF MS. If the bacteria were finally identified as Enterobacteriaceae strains, their hydrolytic activity against ertapenem were further performed by MALDI-TOF MS according to "MALDI-TOF MS based ertapenem hydrolysis assay" that mentioned above. Klebsiella pneumoniae ATCC 700603 provided by Shanghai Clinical Laboratory Center was included as the negative control, while one Klebsiella pneumoniae KPC-2 gene positive strain which identified before in our laboratory was included as the positive control. The conventional inoculation, identification (Microflex LT, Bruker Daltonics) and antimicrobial susceptibility test (Vitek 2 Compact, BioMérieux) of pathogens isolated from positive blood cultures were performed at the same time. Ertapenem, meropenem and imipenem were used as carbapenem agents in the research. The strains were identified as carbapenem-resistant Enterobacteriaceae (CRE) if they were resistant to at least one of the three carbapenem agents.
Molecular assay
The carbapenemase genes, including bla KPC , bla IMP , bla NDM , bla VIM , bla GES and blaOXA-48 were detected by Polymerase Chain Reaction (PCR) and DNA sequencing within the strains which were indentified as CRE [11].
Strains for blood culture simulation
The strains collected here covered 7 species of Enterobacteriaceae, including Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Escherichia coli, Citrobacter freundii, Klebsiella oxytoca and Raoultella ornithinolytica. 32 carbapenemase producers contain 6 kinds of carbapenemases in total, including KPC-2, NDM-1, IMP-1, IMP-4, VIM-1 and VIM-2.
Peaks of ertapenem and the hydrolysed, decarboxylated form
Ertapenem intact peaks [M + H]+ at 476.5, [M + Na]+ at 498.5, [M + K]+ at 514.5, [M + 2Na]+ at 520.5, [M + Na + K]+ at 536.5 and [M + 3Na]+ at 542.5 and the peaks of their hydrolysed, decarboxylated form [Mhydr/decarb + H]+ at 450.5, [Mhydr/decarb + Na]+ at 472.5, [Mhydr/decarb + K]+ at 488.5, [Mhydr + H]+ at 494.5 and [Mhydr + 2Na]+ at 538.5 were all showed in Fig. 1. The potassium ion derived from human blood can combine with both of ertapenem and the hydrolysate and form peaks such as [M + K]+, [Mhydr/decarb + K]+, etc. These peaks were analyzed when mass spectrums of ertapenem solutions incubated with carbapenemase producers or non-carbapenemase producers were imported in Flexanalysis 3.0 software. Carbapenemase producers can be distinguished preliminarily by the following criteria: (1) almost absence of all ertapenem intact peaks; (2) presence of peaks corresponding to hydrolysed, decarboxylated form with high intensity.
LogRQ value calculated with the intensity of peaks
As a matter of fact, the subjective judgment of carbapenemase and non-carbapenemase producers according to the artificial observation of these peaks was ambiguous. We introduced logRQ value to analyze the intensities of these peaks. After 2 h incubation with ertapenem solution, the average of logRQ value of 32 non-carbapenemase producers were − 0.85 ± 0.14, and 32 carbapenemase producers were 0.87 ± 0.55 (Fig. 2).
Cutoff value of logRQ
The receiver operating characteristic (ROC) curve of the method with logRQ values was drawn in Fig. 3. The ROC analysis results showed that the logRQ value was effective in distinguishing carbapenemase producers and noncarbapenemase producers (area under curve, AUC = 1, P value < 0.001). These two groups were discriminated by the cutoff value of logRQ − 0.45 with a sensitivity of 100% and specificity of 100%.
Efficiency of MALDI-TOF MS based hydrolysis assay
A total of 385 clinical blood culture bottles contained Enterobacteriaceae strains from different body parts of 218 patients were collected. 81.3% (313/385) were sensitive to carbapenem, including Escherichia coli (207), Klebsiella pneumoniae (68), Enterobacter cloacae (15), Klebsiella oxytoca (6), Serratia marcescens (5), Citrobacter freundii (3), Citrobacter koseri (3), Enterobacter aerogenes (2), Morganella morganii (2), Proteus mirabilis (2). The logRQ values of all 313 isolates were < − 0.45 with the accuracy rate of 100%. And 18.7% (72/385) of clinical blood culture bottles contained CRE (Table 1). The logRQ values of 62 CRE were ≧ − 0.45, while the other 10 CRE were < − 0.45. Comparing with the detection of carbapenemase genes, MALDI-TOF MS based ertapenem hydrolysis assay of carbapenemase detection has a sensitivity of 92.5% (62/67) [95% CI 86.2–98.8%] and specificity of 100% (5/5) [95% CI 100–100%].
MALDI-TOF MS has been used commonly for direct identification of microorganisms from positive blood cultures in clinical microbiology laboratories. There are various bacterial extraction methods for direct blood culture identification, including blood cells lysis [12,13,14,15,16], differential velocity centrifugation [17] and serum separator tubes usage [18]. Nowadays, MALDI-TOF MS has not only been used for identification of pathogens but also for detection of antimicrobial resistance [19, 20]. In our laboratory, serum separator tubes were used to collect bacterial pellets. After placing the pellets onto the target plate, MALDI-TOF MS was directly performed for identification. This protocol was routinely performed for positive blood cultures in our laboratory. The species identification reports of positive blood cultures usually delivered to clinicians within 30 min. In our previous research, the carbapenemase activity of Enterobacteriaceae strains that growed on Columbia blood agar was detected rapidly and accurately by MALDI-TOF MS based ertapenem hydrolysis assay. Based on that, the carbapenemase activity of Enterobacteriaceae strains in positive blood cultures collected from clinical patients was detected by the same assay. Different from the other researches, only simulated blood cultures were chosen here as the objects [9, 21, 22]. We first established a blood culture simulation method and calculated the cutoff values of logRQ. According to the logRQ values, we test the real positive blood cultures from patients to evaluate the efficiency of this method. The results revealed that this MALDI-TOF MS based ertapenem hydrolysis assay showed more clinical significance than before.
In our study, various species of Enterobacteriaceae strains which contained different types of carbapenemase genes were selected for positive blood culture simulations. These strains almost covered all the types of species and carbapenemase genes that could be detected in clinical microbiology laboratory. Therefore, MALDI-TOF MS based hydrolysis assay here is suitable to detect the carbapenemase activity of all species of the Enterobacteriaceae strains in blood cultures.
The limitation of MALDI-TOF MS based hydrolysis assay is that this method could only be used to detect carbapenemase activity which is one of the most important drug resistant mechanisms of CRE. In our study, 5 CRE strains were identified as non-carbapenemase producer by MALDI-TOF MS based hydrolysis assay. Besides that, no carbapenemase genes which mentioned in Materials and Methods were detected in these 5 isolates. The reason may be the presence of other carbapenem resistance mechanisms [23]. 41.7% (5/12) NDM-1 carbapenemase gene-positive Enterobacteriaceae isolates were distinguished as non-carbapenemase producer with the logRQ values of below − 0.45. However, as reported before, carbapenemase-producing Enterobacteriaceae isolates which carried NDM-1 can hydrolyse carbapenem completely after 2 h incubation [21, 22, 24,25,26]. Pure colonies other than strains collected from blood cultures were taken as research objects in most of these researches. Compared with pure colonies grown on blood agar, the amount of bacteria in pellets collected from positive blood cultures is not easily detected which will lead to the false-negative results. In our study, there were several clinical blood culture bottles which contained two species of Enterobacteriaceae strains. These strains in the same blood culture bottles which all showed sensitive to carbapenem were identified as non-carbapenemase producers by MALDI-TOF MS based hydrolysis assay. Besides, as long as there was one strain in the blood culture bottles showed resistant to carbapenem, all these two strains were identified as carbapenemase producers. The results showed that there were no needs to separate the multi-microorganisms in positive blood cultures when detected carbapenemase activity by MALDI-TOF MS. This method can help to evaluate the effectiveness of carbapenem treatment timely.
The antibiotic sensitivity reports were essential for clinical treatment of patients whose blood cultures were determined as positive. At our laboratory, MALDI-TOF MS has been routinely used to directly identify the species of strains from positive blood cultures. Our data showed that MALDI-TOF MS based ertapenem hydrolysis assay can directly detect carbapenemase of Enterobacteriaceae strains isolated from blood cultures with high sensitivity and specificity. Thus, when the assay is routinely performed for positive blood cultures, we could deliver the reports, especially positive results, to the clinicians within 3 h and provide them evidence to choose appropriate combined medication regimen for the patient early.
MALDI-TOF MS based ertapenem hydrolysis assay is a rapid, accurate, effective and economic method to detect carbapenemase activity of Enterobacteriaceae strains isolated from positive blood cultures. With the high specificity and sensitivity, this method can be used as routine protocol to detect carbapenemase in clinical workflows. So clinicians can acquire meaningful reference early to treat bloodstream infection caused by Enterobacteriaceae strains.
matrix-assisted laser desorption ionization time-of-flight mass spectrometry
carbapenemase-resistant Enterobacteriaceae
Klebsiella pneumoniae Carbapenemase
verona metallo-β-lactamase
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Authors' contributions
Ying Liu, Lisong Shen and Jiajia Yu proposed and designed the study. Jiajia Yu collected, analysed and interpreted the data. Jing Yu, Jingxian Liu, Weinan Zhu and Yuanrui Li participated in proofreading of the manuscript and in critical revision. All authors read and approved the final manuscript.
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The authors declare that they have no competing interests.
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This study was supported by a grant from Shanghai Shenkang Hospital Development Center and Shanghai Health Bureau. The content of this article is solely our responsibility and does not represent any institute's official views.
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Cite this article
Yu, J., Liu, J., Li, Y. et al. Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS. Ann Clin Microbiol Antimicrob 17, 22 (2018). https://doi.org/10.1186/s12941-018-0274-9
DOI: https://doi.org/10.1186/s12941-018-0274-9
- Blood culture
- Carbapenemase
- Enterobacteriaceae
- Hydrolysis


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