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Fast Up-regulation of The LINE-1 ORF2 Proteins in Pulmonary Cells after Exposure to Cigarette Smoke
Received Date: Apr 11, 2018 / Accepted Date: Apr 16, 2018 / Published Date: Apr 21, 2018
Keywords: Human genome; Junk DNA; LINE-1; Lung fibroblast cell cultures
Short Communication
Transposable elements (TEs) make up approximately 45% of the human genome [1]. The biology of the TEs has experienced a remarkable development over the last decade. From being regarded as evolutionary relicts, often called junk DNA, it has become evident that TEs play an important role in shaping and regulating of the human genome. Although TEs exert important physiological functions in the body, they may also contribute to the development of complex diseases, such as autoimmune and neurological diseases, as well as initiate several malignancies and cancers [2]. LINE-1 is among the best characterized TEs in humans. Importantly, LINE-1 is the only TE capable of expressing the entire machinery required for complete transposition and other active TEs move within the genome by L1- mediated transposition [3]. Therefore LINE-1 plays a central role in genome dynamics in both germline and somatic cells [4].
In the lung, LINE-1 activity has been associated with increased natural mutagenesis. Somatic L1 insertions occur at high frequencies in lung tumor genomes and may provide novel oncogenic mechanisms [5]. Interestingly, LINE-1 repression may be lost in response to stressinduced DNA damage [6]. The increased LINE-1 activity is associated with a significant reduction in methylation status, indicating epigenetic mechanisms of gene and or genome regulation [7]. Furthermore, LINE-1 activity may damage the DNA via random insertions and endonuclease-dependent DNA double strand breaks [8]. The LINE-1 ORF2 contains both a RT and an endonuclease together with a carboxyterminal DNA binding domain [9].
Tobacco smoke exposure is also the leading cause of COPD and the main risk factor for the development of lung cancers. In the lung, tobacco smoke induces high degree of oxidative stress, which in turn may induce DNA damage and enhance TE activity. With this in mind we assessed LINE-1 activity in primary human pulmonary cells (fibroblasts and airway smooth muscle cells) before and after exposure to cigarette smoke.
Lung fibroblast cell cultures were established from small sections of lung parenchymal tissue and grown in RPMI 1640 (Lonza, Basel) supplemented with 5% fetal calf serum (FCS), 8 mM l-glutamine, 20 mM hydroxyethyl piperazine ethane sulfonic acid and 1% modified Eagle's medium vitamin mix (Gibco, Paisley, UK). Cigarette smokeconditioned medium (SCM) was prepared by passing cigarette smoke of one cigarette of a commercially available brand (Gauloises Blondes; Altadis, Madrid, Spain) with a 60 mL syringe through 25 mL RPMI medium in a Schott® flask containing an influx and aspiration channel [10]. Prior to addition of SCM, the cells were serum starved for 24 h and diluted SCM (10%) was added in absence of FCS. LINE- 1 activity was detected with LINE-1 ORF2-specific antibodies (Santa Cruz Biotechnology, H-110, sc-67197) and protein expression was normalised to α-tubulin (Santa Cruz Biotechnology).
As demonstrated in Figure 1, resting primary human airway smooth muscle cells did not express detectable levels of L1-ORF2.
Figure 1: LINE-1 protein (L1-ORF2) expression in primary human airway smooth muscle (ASM) cells cultured in absence and presence of smokeconditioned medium (SCM). Cells were cultured in RMPI without SCM (resting cells; t=0 minutes) or in the presence of SCM for 10, 30, 60, 90 and 120 minutes (as indicated). The applied SCM was used as a 10% dilution from a stock solution containing the smoke equivalent of 1 cigarette/25 ml. The Western blot shown is representative for 5 independently performed experiments.
In the presence of SCM (10%), we observed a biphasic LINE1-ORF2 expression pattern: an immediate early maximum at 10-30 minutes and a second maximum after 120 minutes. We observed the same pattern of L1-ORF2 protein expression in primary human bronchial fibroblast cell lines that we established from the same pulmonary tissue. Previously we demonstrated that exposure to 10% SCM induced a complete inhibition of pulmonary fibroblast proliferation [10]. This was associated with a significantly upregulated full-length C/EBPα and C/EBPβ proteins due to a shift in the translational control of CEBPA and CEBPB mRNAs involving regulatory mechanisms independent of DNA transcription. Our current data demonstrate that an increased activity of L1-ORF2 is preceding the SCM-induced block of fibroblast proliferation. Currently, it is unclear why this is associated with an immediate increased activity of LINE-1, but we speculate that it may function as an intracellular warning system to immediately terminate all pro-proliferative signals and enter a quiescent state in order to prevent proliferation- and/ or oxidation-associated DNA damage, including the reactivation of integrated viruses. The immediate abrogation of proliferation may thus provide an innate protection mechanism triggered in response to environmental toxins. Cigarette smoke contains ROS at high concentrations and is therefore a potent genotoxic agent [11].
Further, LINE-1 RNA expression levels detected by TaqMan PCR in 3 cell lines did not show significant differences before and after smoke exposure (data not shown), indicating that the L1-ORF protein expression is controlled at the level of translation. Recent studies demonstrated that TE RNA is constitutively and massively expressed in human cells [12], which enables fast biological responses as observed in immediate upregulation of the L1-ORF2 proteins. The LINE-1
RNA itself is ubiquitously present and thus immediately available for translation into proteins or to evoke a non-coding RNA response to halt proliferation.
It should be noted that LINE-1 protein expression itself does not automatically imply de novo LINE-1 insertions into the genomealthough our data do not exclude this possibility. The detection of de novo insertions of LINE-1 sequences should rather be determined using direct assessment techniques [12-14]. The high expression levels of the LINE-1 ORF2 proteins after exposure to SCM signifies the presence of two fundamental proteins of the transposition machinery: L1-encoded reverse transcriptase and endonuclease. Therefore, an increased activity of LINE-1 elements may also indirectly affect the mobility of other mobile elements, such as short interspersed nuclear elements (SINEs), which rely on the LINE-1-encoded protein machinery for transposition. Although LINE-1 mobilization was able to induce DNA double strand breaks [8], indicating that the endonuclease activity of expressed ORF- 2 is capable of genome destabilization independent de novo insertions, further investigations is required to reveal whether smoking induces DNA damage indirectly through mobilization of SINEs and Aluelements. Taken together, we show that LINE-1 can be reactivated in pulmonary cells, a phenomenon that should be considered when thinking about smoking-induced lung cell proliferation-disorders and developing strategies to prevent and/or treat them.
Supported by the Swiss National Science Foundation (Grant: 310030_133109).
- Levy S, Sutton G, Ng PC, Feuk L, Halpern AL, et al. (2007) The diploid genome sequence of an individual human. PLoS Biol 5: e254.
- Miglino N, Roth M, Baty F, Brutsche M, Tamm M, et al. (2012) Asthma and the regulated retrotransposon transcriptome. Eur Respir J 40: 788-790.
- Dewannieux M, Esnault C, Heidmann T (2003) LINE-mediated retrotransposition of marked Alu sequences. Nat Genet 35: 41-48
- Babatz TD, Burns KH (2013) Functional impact of the human mobilome. Curr Opin Genet Dev 23: 264-270.
- Iskow RC, McCabe MT, Mills RE, Torene S, Pittard WS, et al. (2010) Natural mutagenesis of human genomes by endogenous retrotransposons. Cell 141: 1253-1261.
- Van Meter M, Kashyap M, Rezazadeh S, Geneva AJ, Morello TD, et al. (2014) SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age. Nat Commun 5: 5011.
- Saito K, Kawakami K, Matsumoto I, Oda M, Watanabe G, et al. (2010) Long interspersed nuclear element 1 hypomethylation is a marker of poor prognosis in stage ia non–small cell lung cancer. Clin Cancer Res 16: 2418-2426.
- Gasior SL, Wakeman TP, Xu B, Deininger PL (2006) The human LINE-1 retrotransposon creates DNA double-strand breaks. J Mol Biol 357: 1383-1393.
- Piskareva O, Ernst C, Higgins N, Schmatchenko V (2013) The carboxy-terminal segment of the human LINE-1 ORF2 protein is involved in RNA binding. FEBS Open Bio 3: 433-437.
- Miglino N, Lardinois D, Sadowski C, Tamm M, Borger P (2012) Cigarette smoke inhibits lung fibroblast proliferation by translational mechanisms. Eur Respir J 39: 705-711.
- Yoshida T, Tuder RM (2007) Pathobiology of cigarette smoke-induced chronic obstructive pulmonary disease. Physiol Rev 87: 1047-1082.
- Faulkner GJ, Kimura Y, Daub CO, Wani S, Plessy C, et al. (2009) The regulated retrotransposon transcriptome of mammalian cells. Nat Genet 41: 563-571.
- Singer T, McConnell MJ, Marchetto MC, Coufal NG, Gage FH (2011) LINE-1 retrotransposons: Mediators of somatic variation in neuronal genomes. Trends Neurosci 33: 345-354.
- Shukla R, Upton KR, Munoz-Lopez M, Gerhardt DJ, Fisher ME, et al. (2013) Endogenous retrotransposition activates oncogenic pathways in hepatocellular carcinoma. Cell 153: 101-111.
Citation: Miglino N, Tamm M, Borger (2018) Fast Up-regulation of The LINE-1 ORF2 Proteins in Pulmonary Cells after Exposure to Cigarette Smoke. J Pulm Respir Med 8: 454. DOI: 10.4172/2161-105X.1000454
Copyright: © 2018 Miglino N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Irelands Mark McAuley just won the Longines Dublin Stakes in Dublin over a height of 1,50m. He beat his competition with eleven-year-old Selle Français Utchan de Belheme (Calvaro).
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McAuley won in 39,62 seconds jump-off time, almost one second ahead of US-rider Ward McLain and Noche de Ronda (Quintender). Steve Guerdat and Alamo (Ukato) seized the third place for Switzerland in 40,52 seconds.
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Rights statement: This is the peer reviewed version of the following article: Cranmer, S. (2019), Disabled children's evolving digital use practices to support formal learning. A missed opportunity for inclusion. Br J Educ Technol. doi:10.1111/bjet.12827 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1111/bjet.12827 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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The following five poses root down into the ground, allowing you to expand and create more space within your body and mind.
Practicing yoga outside reminds you to connect with the earth. The following FIVE poses root down into the ground, allowing you to expand and create more space within your body and mind. Hold each pose for 5 breaths.
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From Tadasana, step your left foot back into High Lunge, keeping your back heel raised. Ground down into the earth with your front foot and the ball of your back foot. Engage the muscles of your thighs and hug into your midline. Interlace your thumbs together and from your foundation, reach your arms high into the sky.
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From High Lunge, root down with the four corners of your front foot, reach both arms back, hover your chest over your right thigh and take flight in Airplane Pose. Create more stability by engaging your left leg and foot, pressing back as you square off your hips. The more you press into your standing foot, the higher your heart can soar.
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From Standing Split, bring your hands to your heart, place your right hand down directly under your right shoulder and bring your left hand to your left hip. Stack your left hip directly over your right hip and align your left shoulder directly above your right. Root down into the earth with your standing leg as you lift up and extend out of your raised leg and arm. Expand in all directions. Take 5 deep breaths in Half Moon Pose (Ardha Chandrasana), then release out of the pose by bending both knees and bringing your left foot to meet your right foot. Ground down with both feet as you rise up to stand, arms to your sides in Tadasana. Repeat poses 2–5 on the other side.


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Following the conclusion of ICC Men's Cricket World Cup 2019, Australia is next to host a major global cricket event, with two World Cups coming in 2020. Fans can now plan their year, with ticket pricing for ICC Men's T20 World Cup 2020 revealed.
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Regional News
California's strict gun laws undercut by more lenient, neighboring states
Posted Jul 30 2019 07:24AM PDT
Video Posted Jul 30 2019 07:16AM PDT
Updated Jul 30 2019 07:47AM PDT
GILROY, Calif. (KTVU) - Investigators say Santino Legan purchased the assault weapon used in the Gilroy Garlic Festival shooting Sunday nearly three weeks earlier from a firearms dealer in Fallon, Nevada.
The owner of Big Mike's Gun and Ammo confirmed in a post on his Facebook page that the Gilroy High graduate purchased the AK-47 style weapon over the internet and came to pick it up at the business about 60 miles east of Reno.
"Good people have been hurt and this goes against everything I believe in…We are a small home business, we sell to people who we think are upstanding citizens to promote safe sport shooting," the owner's post stated.
Franklin Zimring, a UC Berkeley Law professor who specializes in gun laws and policy says Legan's July 9 purchase highlights the differences between state laws.
"If he were to walk into a California gun store he couldn't have purchased this weapon because they couldn't sell it to anyone," said Zimring.
California law bans the sale of assault weapons and it is generally illegal to possess assault weapons in the state. California also recently raised the minimum age to buy a rifle to 21 years of age from the previous age 18.
Legan was 19-years-old.
In Nevada the minimum age is 18 to buy a gun and there is no ban on assault weapons.
Also, police said Monday they're still determining where Legan lived. If he was a California resident, California gun laws would apply to out-of-state sales.
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How to Make the Ultimate Slime Easter Hamper
Create the ultimate slime Easter hamper that will keep the kids occupied on Easter Sunday and beyond! This is the perfect gift for a child, and all components are available online and in store at Hobbycraft.
Elmer's Magical Slime Activator »
Arrange the slime components and fillable eggs within the Easter hamper set for a gift that'll be an immediate hit with the kids! Add chocolate snacks and other small treats to fill in any gaps.
To make the slime:
1. Pour out approx 130ml of PVA Glue into the paper cup
2. Add your paint and embellishments! Squeeze in some of the paint, and mix until you reach your desired colour. At this point, you can also mix in any embellishments you want to add such as glitter or sequins!
3. Pour approx. 65ml of Elmer's Magical Liquid Slime Activator and mix with a wooden craft stick until mixture becomes harder to stir and slime begins to form. The 259ml bottle of Elmer's Magical Liquid Slime Activator should make four batches of slime.
4. Take the slime out of the cup and knead with both hands. Manipulate and massage the slime – the more you work it, the less sticky it becomes and more it sticks to itself.
5. Pop the slime into the fillable easter eggs and fill with mini plastic dinosaurs or unicorns for an alternative take on the Easter egg!


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The anvil of justice is planted firm, and fate who makes the sword does the forging in advance.
Category Archive: Aeschylus
A god implants in mortal guilt whenever he wants utterly to confound a house.
I say you must not win an unjust case by oaths.
The one knowing what is profitable, and not the man knowing many things, is wise.
The man who does ill must suffer ill.
Only when a man's life comes to its end in prosperity dare we pronounce him happy.
Death is softer by far than tyranny.
What good is it to live a life that brings pains?
The wisest of the wise may err.
Ah, lives of men! When prosperous they glitter – Like a fair picture; when misfortune comes – A wet sponge at one blow has blurred the painting.


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Customer Reviews
Customer Reviews
Find out how this company strives to cure your "workplace zombies" and boost employee morale!
Healey Green market and sell online products in the garden machinery range including Lawn Mowers, Brush Cutters, Earth Augers, Chainsaws and more. They are passionate about providing quality and customer service to their clients.
Odoo fits all kinds of business! This is a good example of Odoo's flexibility, the Scratch DJ Academy manage their courses and event with Odoo.
Discover how Odoo can improve a teacher's course. The Odoo Education Program is free and available for any teacher.
How ENA saved hundreds of thousands of euros thanks to Odoo
Portugal's annual school teacher allocation handled by open source ERP
Coimbra University to save plenty with open source ERP


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Our next event is our annual trip to Scotland and FrightFest Glasgow. Dates are the 5th, 6th and 7th March. Films and guests will be announced in mid January 2020.
The FrightFest website does not use cookies to store or track any information about your visit, the fans forum does however use cookies to allow posts to be made.
Continued use of both the website and the forum implies that you are happy with our policy
FrightFest is the registered trade mark of London FrightFest Limited. © 2000 - 2019.
This web site is owned and published by London FrightFest Limited.


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- This event has passed.
Lyric Opera of Kansas City's La bohème
November 13
One event on November 13, 2019 at 12:00am
One event on November 15, 2019 at 12:00am
One event on November 17, 2019 at 12:00am
Event Navigation
Paris is for lovers…Puccini is for opera lovers. At the crossroads, an unrivaled romance sets bright bohemian rhapsodies against a tragic trajectory, blending into one of opera's quintessential tearjerkers. It's here, on a brisk, candlelit night, that a young seamstress loses her keys and finds the love of her life.
Lyric Opera of Kansas City: Founded in 1958, Lyric Opera of Kansas City brings high quality live operatic performances to the people of the Kansas City area and five-state region at the state-of-the-art Kauffman Center for the Performing Arts. Our mission is to make Lyric Opera of Kansas City indispensable to the public through transformational opera experiences and broad service which captures the hearts and minds of our communities.


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Journal of International Peacekeeping 13 (2009) 159–180 © Koninklijke Brill NV, Leiden, 2009 DOI 10.1163/187541109X403025 www.brill.nl/joup J O U P A Credibility Gap: Th e Behrami and Saramati Decision of the European Court of Human Rights Heike Krieger * Free University of Berlin, Faculty of
Reflections on Jaloud v. the Netherlands
Jurisdictional Consequences and Resonance in Dutch Society
Friederycke Haijer und Cedric Ryngaert
Organization for Scientific Research under the vidi Scheme. 1 Introduction On 20 November 2014, the Grand Chamber of the European Court of Human Rights (ECtHR) in Jaloud v. the Netherlands 1 held that the Netherlands had failed to adequately investigate the circumstances surrounding the death of an
The Development of Singapore's Military Justice System
Enforcing Discipline and Protecting Rights in a Citizen Armed Force
Joshua Matthew Goh
lacking in Singapore's military jurisprudence. The decisions of the European Court of Human Rights ( echr ) that criticized the uk military justice system formed the catalyst for its civilianization. In Engel v Netherlands (1976) 1 echr (Ser A) 3, 1 ehrr 647 ( Engel ), 60 the echr
Stephan Hollenberg
between the international obligations at issue was resolved is the European Court of Human Rights' ("ECtHR") decision in the Waite and Kennedy case. 55 In this case the Court gave a clear ruling on how the balance should be drawn. It recognized the importance of international cooperation, and
Towards Convergence in International Human Rights Law
Approaches of Regional and International Systems
Ediert von Carla M. Buckley, Alice Donald und Philip Leach
Ediert von Laurence Boisson de Chazournes, Cesare Romano und Ruth Mackenzie
Published under the Transnational Publishers imprint.
Ediert von Laurence Boisson de Chazournes, Cesare Romano und Ruth Mackenzie
Published under the Transnational Publishers imprint.
The United States, the UN, and New Nationalisms
Old Truths, New Developments
Thomas G. Weiss, David P. Forsythe und Roger A. Coate
the UN , preserving alliances, advancing democracy and human rights, and coping with climate change. In Europe, populations resented "Brussels," or EU decisions that went against the grain of national traditions, and "Strasbourg," or the Council of Europe and rulings of the European Court of Human


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8b website builder Free
A few months ago we found this project called 8b which is nothing more than an online version of the mobirise application.
With 8b website builder any user can use the mobirise version through the browser by accessing 8b.
You can log in with the mobirise account, google or facebook and if you want you can make a simple registration.
8b website builder is exactly the same as mobirise, identical!
You choose a follow-up, choose the theme later and redirect to the edit panel as in mobirise, choose blocks and edit texts and images save and you're done!
A pretty cool project, which we do not understand because this project with the face of mobirise won the name of 8b.
I've already sent a message to the team, but no answer yet!


text: View cart "Engraved Clear Roca Tumbler" has been added to your cart. Plum flared vase £32.00 £32.00 Plum flared vase 12cm (d) x 20cm(h) x 10cm(d) In stock Plum flared vase quantity Add to cart Description Plum flared vase 12cm (d) x 20cm(h) x 12(d)cm: Recycled glass Additional information Additional information Weight 2000 g Need Help? Contact Us Leave Feedback Contact Us [contact-form-7 id="2354" title="Milagros contact"] Leave Feedback [contact-form-7 id="2533" title="Milagros contact_copy"] SKU: V2P(T)10x30 Categories: Glassware, Recycled Coloured Glass Tags: coloured, coloured glassware, coloured vase, engraved glass, glassware, plum, two tone coloured glassware, vase Related Products £18.00 Add to cart Lime Green Jug (Straight) £18.00 £18.00 Add to cart £10.00 Add to cart Engraved Clear Large Flared Tumbler £10.00 £10.00 Add to cart Out of Stock £8.00 Read More Clear with blue rim wineglass £8.00 £8.00 Read More £32.00 Add to cart Engraved Clear Carafe £32.00 £32.00 Add to cart £8.00 Add to cart Clear wine glass with yellow rim £8.00 £8.00 Add to cart £9.00 Add to cart Engraved Grape Roca Tumbler £9.00 £9.00 Add to cart £12.00 Add to cart Engraved Grape Wine Glass £12.00 £12.00 Add to cart £9.00 Add to cart Engraved Grape Straight Tumbler £9.00 £9.00 Add to cart


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Bernie Sanders says that the Israeli government is racists. Well, if you think someone being biased against people shooting missiles into your country is racist, then Bernie is right. Evidently, Bernie Sanders favors terrorists over anyone who is not a commie.
In Israel, Arabs are allowed to vote or run for public office, but on the West Bank, any country named Israel or individual Jews have no right to exist. bernie says there needs to be a level playing field. Okay, is he proposing then that Israel send terrorists into the West Bank to blow up buses, shoot missiles into civilian populations and add an amendment to their constitution declaring that Arabs and Arabs countries have no right to exist?
"You've been an outspoken critic of Israeli Prime Minister Benjamin Netanyahu, yet Israel is also one of America's most important allies in the world. Given that Prime Minister Netanyahu just won another term in office, how do you plan to maintain the strong U.S.-Israel relationship despite those critiques?" asked Shelly Tsirulik, a Harvard student in the audience.
"As a young man about your age, I spent a number of months in Israel. I worked on a kibbutz for a while. I have family in Israel. I am not anti-Israel but the fact of the matter is that Netanyahu is a right-wing politician who, I think, is treating the Palestinian people extremely unfairly," he began.
"So what I believe is the United States gives billions of dollars in military aid to Israel. What I believe is not radical. I just believe that the United States should deal with the Middle East on a level playing field basis. In other words, the goal must be to try to bring people together and not just support one country, which is now run by a right-wing, dare I say, racist government."
Netanyahu won a highly-contested general election in Israel earlier this month, paving the way for him to serve an unprecedented fifth term.


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"Music is what feelings sound like out loud. I sing songs that speak from my heart. They tell my story, how I feel."
"Nothing speaks louder than your heart. Listen to what it's telling you."
"You might as well be the devil." "Baby, you're the one holding the apple."
"I didn't love you the day I met you, or even a month later. But somewhere between hello and the goodbye I'm unable to bear"
"Don't leave. I want you to be with me." "You'll have your family." I swallow before I say the words that will change this relationship forever. "You're the one I need." "But that would mean meeting your family." It does, and I'm okay with that if it means she's by my side. "I don't care. I need you to be with me." She smiles and cradles my face with her hands. "Of course. I'll come if it's what you want, but this is going to change everything." "I know, but it's what I want."
"I'm sorry for all the secrecy, for making you feel like you weren't important enough to know the real me. But I'm most sorry because I have fucked you – I don't know how many times – and never made love to you."
"I love her too much to let her leave and I need to tell her right this minute."
"She has bewitched me. And I want her. She's the one."
"We've shared countless kisses. They were almost always heated and demanding, but this one is entirely different. It tells me things he can't or won't say because it goes against everything he intends for our relationship."
"I've known pain my whole life, but this is a new kind for me. It isn't born of something wrong or ugly. This pain is conceived out of beauty— my love for Jack Henry McLachlan. I embrace it. I clutch it as tightly as I can with both fists because I never want to forget the love I have for him. Loving him will forever be my Beauty from Pain."
They agreed on three months…but their love knew no boundaries.
Jack McLachlan is a winemaking magnate and easily one of Australia's most eligible bachelors. His success and wealth make him no stranger to the complications of romantic relationships and that's why he goes to extreme measures to avoid the hassle. He prefers simplicity in the form of a beautiful female companion with no strings attached. He arranges relationships like business deals and they're always the same. No long term relationships. No real names.
It's his game and his rules. He's content to play as usual, but when Laurelyn Prescott enters his life, his strategy must change because this player is like none he's ever encountered. His world is turned on its head after he begins a three month affair with the beautiful American musician. Nothing goes according to plan and as he breaks more and more of his own rules for her, she's exceptionally close to becoming something he never thought possible. His ultimate game changer.
Not recommended for younger readers due to sexual content and language.


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Market Gardener Training Program
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EPN Breakfast- January 14, 2020 The Bourbon Barrel Connection: Revitalizing Appalachian Ohio economies and oak-dominated forests
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+ Follow COMPREHENSIVE FIREARMS AND AMMUNITION REGULATION ACT Tag
By Mylen P. Manto | January 14, 2016 - 9:00am
By Malou Escudero | November 13, 2015 - 9:00am
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Latest update December 9th, 2019 12:59 AM
"We will make it up to them in the years to come," said Minister of Social Protection, Ms. Volda Lawrence, yesterday, when asked if she had made any representation at the level of Cabinet for pensioners to have a Christmas bonus this year.
The Minister's disclosure was forthcoming during her Ministry's end of year press conference which was held at the Millennium Manor Hotel, Hadfield Street, Georgetown.
Government, earlier this month, announced a one-time $50,000 payout for workers in the public sector who earned a monthly income of less than $500,000. For a number of years, public servants were able to receive increases to their salaries in December. The increases were retroactive to January of the said year. Pensioners would usually also benefit from the stipulated percentage increase.
However, after being elected to office in May, the coalesced A Partnership for National Unity + Alliance for Change (APNU+AFC) in its national budget announced an increase for public sector workers retroactive to July 1.
Moreover, as the Christmas season approached some public servants had vocalised their concerns, they would not have been eligible for a year pay package. This year was perhaps the basis for Government's decision to pay them a $50,000 Christmas bonus. More than $1.5 billion was paid to some 30,700 public sector workers including nurses, teachers, members of the disciplined services and semi-autonomous agencies. But several pensioners voiced their concerns that they were not taken into consideration when Government made its decision.
Minister Lawrence said, "The Finance Minister sits next to me in Cabinet, so you can understand his predicament. Since the proposal was made seeking to find monies to pay workers the $50,000, immediately I asked about the pensioners and I was told by the Finance Minister (Winston Jordan) that was not possible at this time, but if I could have found the money in my budget he was willing to consider it."
She however informed that unspent monies allocated to her Ministry were already earmarked for several important projects at some homes and various offices that fall under the purview of the Social Protection Ministry.
"So we knew that we too couldn't find that money to give to our pensioners. We have been speaking to them everywhere we go. I have been assuring them that their interest is our primary concern…" asserted Minister Lawrence.
The Minister, in response to yet another pensioners-related question at yesterday's press conference, sought to clarify what she described as a "misconception" regarding the subsidies that were previously paid. Pensioners under the People's Progressive Party Civic Government were entitled to subsidies for their water and electricity consumption.
Minister Lawrence stressed, "There was not a community that I went to personally, where senior citizens have not come to me and addressed the issues by saying 'thank you Minister and thank you and the Government for ensuring that there is a level playing field in the pension'."
The Minister in her deliberations called into question, the knowledge of some media operatives who have reported on the issue and even questioned whether comments from pensioners across the country were sought before reporting on the stopping of subsidies issue.
Minister Lawrence went on to disclose that upon hearing the announcement of the subsidies offered in the National Assembly in 2012, she (then Shadow Minister of Human Services and Social Security) had some concerns with it. Her concerns were premised on her awareness that it would not have catered to the entire senior citizen population.
"Immediately I wrote the (then) Minister of Finance, I copied it to the Speaker (of the National Assembly), the Leader of the Opposition; I copied it to the Minister then of Human Services and Social Security because I felt it was a very unfair decision," said a passionate Minister Lawrence yesterday.
She qualified her disclosure by pointing out that in some areas of Guyana, pensioners do not have access to Guyana Power and Light (electricity) and Guyana Water Incorporated (water) services and even if they did, the services would have had to be registered in their names.
"The records of the National Assembly can testify that despite 42,000 plus pensioners were allocated for…in response to my question (posed) to the then Minister there were only 7,000 pensioners who were accessing that allowance (subsidy)," declared Minister Lawrence.
"It started off being very biased and I was very happy when the current Minister of Finance listened to what I had to say and agreed. He ensured that we placed all of those monies across the board for each pensioner," said Minister Lawrence.
In his presentation of the national budget earlier this year, Finance Minister, Mr. Winston Jordan, announced an increase of Old Age Pension from $13,125 to $17, 000. This, therefore, means that instead of being granted the subsidies, all pensioners have been granted an increase to their pension. It is not for the few, it is for all."
Dec 09, 2019Calvin Chapman story and photos Reigning Caribbean Basketball Confederation (CBC) champions, Guyana, maintained their dominance over the island republic of Grenada on Saturday night to go two up and...
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Dec 09, 2019
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Dec 09, 2019
My friend David (Hinds) in his column yesterday wrote that I misquoted him as adumbrating support for the present regime... more
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By Sir Ronald Sanders There has always been tension between encouraging foreign investment and promoting local entrepreneurship.... more
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Pre-Owned Cars on Woodward
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Gods, demon lords, and alignment. Why do we keep the priests of demon lords around? They must useful to somebody! I like the ambiguity of the old gods. They are not all good or all evil. And I like the old threefold alignment: Law, Neutrality, and Chaos. I think alignment languages and thieves cant are just signs and the use of particular words in particular ways. If you say something and I answer "Amen!" then I'm not just saying "Yes!" I'm also saying that I'm a Christian. That's how I understand them.
But most of all, I just love the statements of the priests of Chaos and the priests of Law. I still do.


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Regulator aims to stabilize nation's housing market
As Chinese property developers are flocking to issue offshore bonds, the nation's top regulator has stepped up efforts to prevent financial risks and stabilize the real estate sector, said experts. Chinese real estate enterprises issued 604.7 billion yuan ($87.9 billion) worth of bonds domestically and overseas so far this year, among which $38.4 billion were offshore, reaching a historic high in terms of volume, and it is approaching the total amount of 2017, according to a report by Chinese financial information service provider Wind Information. Read more on: http://global.chinadaily.com.cn/a/201907/17/WS5d2e79f3a3105895c2e7ddd2.html/


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Mzambri needed a new web page design and created a contest on 99designs.
A winner was selected from 247 designs submitted by 36 freelance designers.
Yoga - fast flow - we have a cozy studio (called the YoGarage - a converted garage). I am catering to area moms and teens. We have a small studio, offering personalized attention. Think warm, welcoming, fresh and hip. Our tag line is "yoga for every body", with the "every" bolded. We don't want people to worry about body image, just enjoy the amazing benefits of yoga - freeing body and mind. We are pretty laid back - no stress. I have a logo.
The website will be used to:
1. tell people about our company
2. allow them to easily sign up for a class
3. keep them coming back for more!
no but I have a domain
I have a logo which I can send. That should set the tone for color. I want the design to be very stream-lined and minimalist. Zen.
The site should convey peace and contentment. Type should be soft and rounded. Please stay away from photos that show the face
Home page - this should have an area to catch the reader's attention and draw them in. Room to explain who we are - our mission, etc.
Payment page - sign up for classes and pay
Extra page to describe class levels, benefits, etc.
nothing cliche when it comes to yoga.
i like the posezen to be in lower case - with the two colors. Tag line is "yoga for every body". I want a very fresh, clean site. Not a lot going on except to sign up for class.


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Roxas City girls. , President Manuel A. This article is about pres the Filipino president.
Malacanang Museum and Library ( Manila). Manuel Roxas until. campaign materials and paraphernalia dating back to the time of Manuel Roxas, the fifth. Spanish City Bridge ( Minor Correction) The Capizenos and Roxas City residents should try there best to preserve this historical structure for posterity.
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The word " there" is wrong, it should have been " their". Pinay Romances is one of the fastest growing online dating Philippines websites for matching Filipina and western men. We are based in the Philippines.


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copper mining in angola
Conserving resources, enriching lives, creating value Mining and .- copper mining in angola ,Mining and Metal Industry in Angola Conserving resources, enriching lives, . The country'.s undeveloped mineral resources include copper, gold, iron ore, lead.New push for iron and copper mining mega-projects in Angola - IPIM12 Apr 2010 . Luanda, Angola, 12 April - Angola is once again moving ahead with the mega mining projects for extraction and processing of iron and copper.
Angola Mining Report Q1 2013 | Market Research
21 Nov 2012 . Angola Mining Report Q1 2013 Angola'.s mining industry is set to reach . New areas of mining including iron ore, copper and phosphatesare.Dapatkan kutipan
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Mining in Angola is an activity with great economic potential since the country has one of the largest and most diversified mining resources of Africa.Dapatkan kutipan
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Promoting investment in African mining exploration, establishing partnerships between mining companies and encouraging collaboration between Australia and.Dapatkan kutipan
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Angola'.s mineral industry was dominated by diamond and petroleum .. ore mine, the possibility of redeveloping the country'.s copper deposits, and the.Dapatkan kutipan
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17 Aug 2012 . AP Services and genius Mineral of Angola has planned to start copper exploration activity in Mavoio mining region located in Uige Province.Dapatkan kutipan
Angola to restore copperbelt rail after 40-year halt - FAST NEWS .
22 Mar 2013 . Companies that mine copper on the so-called copperbelt that straddles the border between Congo and Zambia include Barrick Gold Corp..Dapatkan kutipan
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Mining companies in Angola, via MiningIQ, provide information for tendering and mine life cycle phases for effective management of mining projects in Angola.Dapatkan kutipan
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19 Mar 2014 . The project to extract copper from Mavoio is under way in accordance with technical and scientific requirements and its dynamics are suitable.Dapatkan kutipan
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22 Jun 2010 . Angola is located on the western coast of southern Africa. It has eighteen . for stratabound polymetallic copper ores of the copper belt type.Dapatkan kutipan
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20 Nov 2013 . ASX-listed Rift Valley Resources has moved to acquire non-listed Sable Minerals, which holds a majority interest in the Ozango copper-gold.Dapatkan kutipan
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Copper production has declined to 1.96 kg in 1963, down from 54.56 kg from the preceding value, . Mining Companies . Angola : history of copper production.Dapatkan kutipan
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8 Nov 2013 . The mining industry in Angola is growing at a stable rate and is . in recent years, Angola has started focusing on the mining of copper and.Dapatkan kutipan
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16 Oct 2013 . Railway companies from Democratic Republic of Congo and Angola will meet to discuss linkages that would allow Congo'.s mine operators to.Dapatkan kutipan
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Louiswho previously held promotion posts with independent distributors. The company was formed after Schlachter reached an agreement with Pye Records, U. The discussions regarding the acquisition of some of the artists from Pye Records began when Pye, U.
Agreement was reached for Prelude Records to acquire the following artists: Both Lorraine ri - saura and Jumbo had just had albums released on the Pye label but both of these albums will now be taken off the Pye label and immediately re-released on the Prelude label. American Song est as the primary liaison between the label's pop artists and the various departments within the company that are directly involved with product development: As with Kalodner and Silva, he will also be responsible for the consideration of specific pop acts for the label, via live auditions, demo tapes, etc.
Prior to this appointment, Kalodner, Silva, Schoenbaum Individually, these appoint- Schoenbaum spent three years at ments aim to continue the com- Chappell Music, where he had pany's long-standing policy of as- served as general professional suring maximum communications manager.
Kalodner out respectively by the directors joined the company in its publicon each coast. Louis Bramy for Spreadeagle Productions. Initially it will serve the motion picture industry and legitimate theater as well as the catalogues and copyrights of estates and of artists not being exploited today.
Deutch will be involved in obtaining recordings as well as administering material in the aforementioned entertainment areas. Within the motion picture Industry, Deutch will work with film producers and national and independent film distributors in several ways.
Buttermilk Sky will also offer similar services to the legitimate theater industry where they will generate recordings on behalf of Deutch Debuts Buttermilk Sky irm the composers and producers of the shows. In that position he was responsible for the formation and building of the company to major status.
Prior to that association, Deutch was with United Artists Music Publishing for 11 years where he rose to president. Consistent with these plans, Midland has beefed up its staff, created a West Coast presence, promoted internally, and added additional space to their recently expanded New York offices.
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Additionally, Noel Love has been named vice president and general manager of the label, and Howard Rosen has been named vice president and director of promotion. Denny Zeitler will be west coast promotion director, Lois Kennedy has been appointed Midland's director of promotion for the New England area. Company Sunset Blvd. CA Music Craft Dist. In the tradition of the few breathtaking vocalists whose remarkable talents immediately catapulted them to the top, Jennifer Warnes is marked for stardom.
Her long-awaited Arista debut is a wonderful album that marries her brilliant vocal performance and a collection of beautifully produced songs. An indelible first impression.
It seems that it all started in when "It's All Over Now," the first song cut by his group, The Valentinos, barely entered the top after the song was simultaneously covered by the Rolling Stones. They, of course, had the hit. Womack claimed that Mick Jagger never reciprocated by writing a song for him, but rather invited Womack to open the show on a recent European tour. Womack has never met the group, but has certainly heard of them.
They beat him to the punch with one of his own songs when they recorded "I Can Understand It" on their first album and subsequently released it as a single. When Dean riedman's debut album is released on Lifesong in a few weeks, there will be those who will be disturbed by the overt sentimentality of his songs.
One sensed, during a short set he performed in a private apartment on Riverside Drive, that riedman has a good understanding of the need for comic relief between the more serious of his "growing pains" songs. A quick, wry sense of humor pervaded his between song patter and some of the tunes including the lighter ones that sound pretentious on the album come off naturally sincere in person.
Perhaps most impressive for a first time professional, however, was riedman's self-confidence. Again, based on this brief solo performance, one sensed the right attitude-involved enough to know what it takes to achieve his goal, detached enough not to let himself be overwhelmed by it.
The maturing process, whereby riedman tries to tie the writing, performing and recording together, will be one to observe. Boykins, Ed Wright of G. Communications, a former president of NATRA and a veteran radio executive before starting his own consulting company, will chair the session.
Ewart Abner, former president of Motown Records Corp. Abner's association with the music industry goes back more than three decades. In addition to the presidency of Motown Records, he was president of Vee Jay Records, a Chicago based black music company, for many years.
Included in the panel will be Clarence Avant, president of Tabu Records; Kenny Gamble, president of Philadelphia International Records; LeBaron Taylor, vice president, marketing, special markets, Columbia Records; and Jerry Wexler, for more than 25 years a producer and executive with Atlantic Records, whose close association with Aretha ranklin and other black artists is well-known throughout the in- Marmaduke of Western Merchandiser, will represent the merchandising segment of the industry.
He was selected to serve on the panel since his company, in conjunction with CBS Records, successful campaign to increase the sale of black music in Western's retail outlets in West Texas and Oklahoma. In addition to the industry members on the panel, Eugene J. Previewed at the meetings were forthcoming albums from three artists newly signed to the label including, Molkie Cole, a Cleveland based rock group; Charlie, progressive rock and rollers; and Steve Hill, formerly of Bloodrock and now a solo artist.
New singles on Janus from Symbol 8 and Light Heart were also debuted during the meeting. Other forthcoming releases discussed included records from Camel and Kayak, slated for release shortly.
CBS Records rang in the New Year with a party at the Bistro in Beverly Hills to thank friends and associates for being part of the company's best year ever. Included are Gene Kelly's dance shoes from "An American In Paris," a familiary crushed hat donated by Jimmy Durante; Victor Herbert's stand-up writing table; Sammy Cahn's typewriter given to him by Lou Levy ; Rudy Vallee's megaphone; ats Waller's piano; a collection of music boxes, and a host of personal notes and signed lead sheets.
The items are on loan from private and public collections, including the Library of Congress. The Hall of ame is located at 1 Times Square, N. Eddie O'Loughlin of Midsong Music said this week that the company is planning a major expansion, including the opening of a west coast office and the addition of two professional managers on the east coast.
Midsong is the publishing division of Midland International. Last week, RW's cover picks included two songs by Jeff Barry and Ellie Greenwich, the early '60s king and queen of dynamic courtship.
A lot of people have been wondering when Greenwich will finally release another solo album. According to Notable Music general manager Eric Colodne, it's the first time since "Mame" that a title song has been recorded by a major artist before the play opened. It was written by Cy Coleman and Michael Stewart The song won the amateur easy listening category in The American Song estival And speaking of the estival, the awards ceremony last week at N.
Better still was the after -party party for the category winners. Even those who were denied the amateur and professional grand prizes had high praise for the festivities because of the number of industry types they met during their two day stay.
Cory Robbins has left his position as professional manager at Midsong Music The re-signing marks the 15th year Campbell has recorded for the Capitol label.
He has had 44 singles and 32 previous albums on Capitol Records. At the same time, Greenberg has announced that Herbie Mann, the original director of Embryo Records during '71, will reassume the post, effective immediately. In this capacity, he will serve as producer or executive producer of all product issued on Embryo, just as before.
Headquartered at the New York offices of Atlantic Records, Mann will oversee every aspect of business conducted by Embryo, including studio production, manufacture, promotion, publicity, sales, merchandising, advertising, artist relations and international operations.
In his new post, O'Brien will be responsible with Scott for the day-to-day administration of the ABC -distributed label, as well as coordinating the radio promotion staff. In addition, he will work closely with Sire Records' co- owners with Jem of Passport production and artist relations staff. O'Brien previously worked with Passport's parent corporation, Jem Records, rock importers, as national promotion director, and most recently served in the same capacity for ATV-Pye Records.
The release includes "In light," the second Warner Bros. Merchandising aids produced in conjunction with the release include a two album sampler produced and assembled by Robin Rothman which will be distributed to key accounts for in-store play as well as to radio and press.
Other materials include a multi - pocket corrugated step down bin, plus mobiles, posters, divider cards, salesman's guide and single sheet order form.
Produced by Gentle Giant. Now the whales are back in a new album and according to Dr. Roger Payne, "There's something about these sounds that appeal to everyone. People of all ages and all walks of life and from all countries turn onto it. It's a thing of beauty.
He shared his musical ideas with his musical partner, Stu Gardner, who took them and added some of his own. The result is music that ranges from light, airy, spacey improvisation over a solid framework It contains the theme of love in each of its thirteen tracks, and as usual, Harley produced the album himself. Maze is unique because they combine the "Philly Sound" with more funky San rancisco sounds Maze will amaze you! Hook, makes his solo album debut with this country -oriented record.
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Her voice is at times reminiscent of Melanie and that radiance should shine through. Silvetti Barnegat, BMI 2: It's still a great song. The rhythms are guaranteed to put pep in your step.
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STIs – Out of Sight, Out of Mind?
By: Dr. Abigail Joseph
A couple years ago, there were talks of a color coded condom that would change color according to the type of Sexually Transmitted Infection (STI) that it would come in contact with. Three teenagers from England won the Teen Tech Award for this brilliant concept. I mean could you imagine how wonderful it would be? Wow, the condom that changes color! (sarcastic tone). But then I thought to myself, how much would this cost? How does it work? Who is it detecting – the user or the receiver? What if there are multiple infections present? What STI would "brown" mean? After all if several infections are present I would think that too many colors would turn dark somehow. SOOOOO yeah the idea was great until I started asking the questions.
The truth is, young people are not thinking about condoms. Yes maybe they would buy a couple of them for fun in the beginning, and after paying the price…which I assume would be very expensive considering it would need antibodies on both sides of the condom and probably some sort of material that is thin enough for sensation but thick enough for protection, I guarantee you it would be more than the normal price. BUT why am I even babbling for? It is rare when you find young people using condoms. Heck, adults don't use condoms!
There is always the "I don't like how they feel," "my partner will think I'm cheating if I ask to use them," "it's too tight" (are you aware they come in different sizes?) or "I trust my partner;" the last one is a very interesting statement because A LOT of the people that claim they trust their partner also trust their side chicks or side kick (it has become such a norm that many of you probably don't get how twisted that is).
Let's talk about the infections shall we? The infection is NOT the initial problem. The laid back philosophy of it "not happening to me" and the naïve concept of "seeing is believing" is what's up. You may feel as though this column is about forcing you to use a condom. For those of you who feel that way I implore you to read it a second time. Changing the way we think is a very important aspect of protecting ourselves. Seeking to be more educated about the unknown is the best way to tackle problematic situations. When we learn about diseases and understand what we are looking for, we are better prepared to proceed.
According to the World Health Organization it is estimated that over 1 million are infected with an STI daily. Young people make up a large percentage of this group and as such targeting our young people and teaching better sexual habits, healthy foreplay, healthier choices and phasing out this wham bam thank you ma'am in the darkness behavior is just as important. When people think about sex education our mind automatically goes to a banana and a condom and we feel as if yeah we already know that, and we brush it off. We have the idea, we understand the concept but we never implement it, OR we use it once just to see how it works. So why are there so many people infected daily? Well apart from promiscuity and lack of condom preference there are unhealthy habits that we practice. We've established that we "love and trust" our partner so we rarely inspect each other. Women tend to be insecure about their bodies whether they are fat, have scars, stretch marks, etc…while men are mostly just insecure about penis size; while they may not say it often they wouldn't question the request of turning out the lights when getting ready for that intimate moment. Your insecurities can become your biggest downfall.
How can you see if there is an abnormal discharge coming from the penis or the vagina? Were there any sores? Is there old scaring from healed sores? Are there warts hidden in the pubic hairs? How sure are you that the bumps around his penis head are pearls and not genital warts? Did you know that a woman's vagina is not the outer part you look at but rather the canal itself? Sometimes the external genitalia of a woman can look nice and pink, smooth even – if she over grooms: peel. It's important that you understand that warts are not limited to the outside skin and may be found inside the canal; and here you are thinking it's ribbed for your pleasure. Women, we do ourselves an injustice when we offer to have sex in the dark, and even more so when we avoid having a regular pap smear done.
A pap smear allows the doctor or nurse a clear visual of your cervix which is tested for cancer. This procedure also gives a visual of your vaginal canal: its color, if there are warts present, if the cervix has fungus or discharge that is thick and so sluggish that it does not drain down and stain your panty for you to even know you have a discharge. Some women are of the habit of douching to get rid of odor instead of seeing the doctor. The odor could be a sign that something is present, as bacteria often carry a smell. Douching and shaving may increase your risk of contracting an infection as it removes good flora and shaving before sexual encounters causes micro tears and breakage in the skin which is your primary line of defense and barrier. I am not saying you shouldn't douche or shave. I'm saying see a doctor if you have odor or discharge and do not shave immediately before an encounter. Micro tears and anticipated friction are a bad combination. Anal sex has become a practice for both heterosexual and homosexual partners, and often times the sentiments are that since it's the rectum, and condoms are generally viewed as protection primarily against pregnancy, it is often ventured unprotected. The rectum's lining is thinner than the vagina. It lacks the cells that create lubrication. These factors along with increased blood flow makes the risk of contracting an infection high.
I can go on and on about little habits we have that increase our risk of contracting infections. We fixate generally on HIV/AIDS and feel as if once we've done our HIV test we are OK. But the reality is that there are many bacteria, parasites and viruses that are capable of transmission sexually. 8 of these pathogens contribute to the greatest incidences recorded worldwide; 4 are presently curable: syphilis, gonorrhea, trichomoniasis and chlamydia; 4 are currently incurable: Hepatitis B, HPV, Herpes and HIV. Condoms are not Armor. Educate yourselves on signs and symptoms. Modify your behavior to protect yourself. YOU are your first priority.


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ADELAIDE, AUSTRALIA – JUNE 08: Rory Sloane of the Crows celebrates a goal during the 2019 AFL round 12 match between the Adelaide Crows and the GWS Giants at the Adelaide Oval on June 08, 2019 in Adelaide, Australia. (Photo by Matt Turner/AFL Photos)
After being elevated to co-captain in the pre-season, Rory Sloane was one of seven Adelaide players to play all 22 games in 2019.
The courageous midfielder led the Crows for contested possessions (288), hard ball gets (95), clearances (131) and tackles (129), ranking Top 15 in the League for all four categories across the home and away season.
Sloane was also number one at Adelaide for player pressure acts (542), placing him equal fifth in the AFL.
A tackling machine 💪 Sloaney goes to the top of the AFL leaderboard for tackles in 2019 #weflyasone pic.twitter.com/gUD4diRKH3
— Adelaide Crows (@Adelaide_FC) May 12, 2019
At a glance:
Games: 22
Goals: 11
Disposals per game: 24.6
Clearances per game: 6
Tackles per game: 5.9
Best game:
Round Eight v Port Adelaide
The consistent Sloane recorded triple-figure player ranking points in 14 of his 22 games, meaning there were plenty of options to choose from.
He was influential in wins over Sydney and Richmond, and notched big numbers in losses to Hawthorn and West Coast.
But it's always tough to look past an impressive Showdown performance.
With Port Adelaide charging home in the final quarter of the Round Eight clash with five unanswered goals, Adelaide needed its leaders to stand up.
A spirited Sloane took two critical marks in the last term to help inspire the Crows to victory, to go with 10 disposals, five inside 50s, two tackles and 198 metres gained in 33 minutes of football.
He finished the game with 26 disposals, nine inside 50s, a game-high 13 tackles and one goal and was awarded six AFL Coaches' votes by Don Pyke and Ken Hinkley for his efforts.