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Introduction Sell My Courses Online
If you are Considering selling memberships, digital Downloads, or online courses, several options are offered for you. One of these has come across my radar called Podia. It's a system intended only for constructing a site in one of those 3 categories–that is pretty cool considering it is possible to cut out the rest of the jumble of e-commerce. In this Podia review, we'll outline the best attributes, along with areas like templates, pricing, and marketing. Sell My Courses Online
Click here to subscribe to Podia today!
Upon first glance, Podia offers a clean interface to get Designing your site. It is easy enough for beginners, and because it is just for memberships, digital downloads, and online courses, the choices are somewhat more consolidated for users in those industries.
The pricing looks somewhat steep, but it is Not Really That bad. In addition, the default theme formats your website with ease, offering an elegant design and easy web design components.
Thus, keep reading to learn more about Podia and to See if it's right for your site.
Services Offered
Unlike many Crowdfunding websites, Podia does not direct you to choose a particular campaign specialization (technology, games, songs, etc) if you register. Rather, they simply prompt you to set up a membership. Those who want your articles subscribe to access to it in exchange for a monthly subscription. You are also offered the opportunity to sell online courses and digital downloads on a one-off basis. If you are in the company of generating digital content and monetizing it, this gives you greater flexibility in how you need to supply your creations than does Patreon.
The Best Features and How They Function
As for the internet course features.
Everything starts with a basic theme, which looks contemporary and clean. You get assistance for an infinite number of classes, clients, and sales. The trickle material is ideal for sending out courses and lessons over a certain time frame. Not to mention, you can upload almost any sort of document to your online class.
One of the main reasons I enjoy the Podia online Course module above some competitions is because it's a built in sales page for amassing money and handing out coupons. Not only that, but Podia includes a presales page if you'd like to pitch your courses and collect email addresses from potential students.
Characteristics for a membership site.
First of all, Podia lets you create and provide Numerous plans with various rates, content, and perks. You are able to stay in contact with your members by sending out emails or even resorting to pictures, video, and audio upgrades.
Bundling products is part of the membership package, and It supports both electronic downloads and internet classes.
Podia helps you construct a Gorgeous membership Website With no need for coding abilities. It supports a wide selection of file uploads (video, MP3, text, PSD, PDF, and more).
Ultimately, each membership material page is organized In a reasonable manner, and the members may communicate with admins and other members in the remarks area.
Email advertising attributes.
Email marketing is actually incorporated into the other Modules such as digital downloads, online classes, and also the membership websites. So, if you want to retrieve emails from prospective (and current) users and students, it's possible no matter what.
The drip attempts are sent out automatically through emails. Podia also offers email newsletters for if you'd like to put together some content and keep people engaged. Tracking is done by checking the click out rates, open rates, and unsubscribe rates. Last, the email advertising tools consist of opt-in forms for putting in your own storefront and prelaunch pages. Sell My Courses Online
Click here to sign up to Podia today!
Digital downloads features.
The digital downloads attributes include things for selling Anything from eBooks to music. Some of the files it's possible to sell include checklists, PDFs, eBooks, videos, sound, and cheat sheets.
The quick checkout is optimized to enhance your Conversion rates, along with the checkout process doesn't require folks to make an account.
Marketing and sales are made simple with the free Sales page. Here, it is possible to insert a movie, FAQ, and email opt-in form. In the end, there's obviously a feature for selling your digital downloads, but you could also flip the switch to provide the things away at no cost.
Templates and Layout
As we spoke about briefly, the templates are preset for Producing your checkout, sales page, membership webpages, and track pages the best they can be. The good thing is it's one of the simplest design processes I've ever seen.
Your course material, and whatever other modules you place On your site, are organized for you. Furthermore, the preset templates look amazing.
I'd say this is going to frustrate some specialist Programmers who need full control over their layout environment. But, it is still a wonderful solution for all skills levels because of how quickly the Podia builder puts together your website.
Ease of Use
Since Podia is provided to you for 30 days without Any charge card, it offers you an opportunity to play around with the resources and find out how easy it's to use.
I'd assert that Podia has the perfect user-experience For novices, intermediates, and advanced users. It makes sense that a number of developers would want to utilize Magento or WordPress for more advanced features, but I really don't see the point if you're only going to make something such as a membership or internet course website.
The hosting is contained, and the dash is fitted with Some fun and simple to comprehend steps for making your website. Consequently, if I wished to create an online course, I'd begin with the Online Course button.
Then, the majority of the attributes are consolidated on 1 page, with a few tabs for your sales page, settings, and sales. It shows the number of sales you have currently brought in, and you can click the Preview button to have a peek at your website.
The primary buttons include the options to Add Files, Add A Link, and Add Text. That's pretty much it. I know for a fact that a few people will not be pleased with the limited design alternatives, but that is kind of the purpose of Podia. It cuts down on the complex process of building an internet course or membership site, and it is all done by providing you a beautiful motif to start off with.
The payments have been processed through PayPal or Stripe. You can choose. Sell My Courses Online
Although this is extremely restricted, Stripe and PayPal Cover a wide array of countries. You shouldn't have any problems with the fees or services.
Marketing is built in with the email advertising module. This sends everything out from course upgrades to email newsletters. What's cool is that you can brand them to the best of your skill and select whether you want to send out the manual or automated messaging. The drip content is configured on the automatic end of things.
In addition, a sales page is provided for listing a Item description, headline, and cover photograph. I enjoy this for people that wish to link from ads on Google and Facebook. Feel free to upload a debut video and make an FAQ region. You can incorporate your shopping cart and email marketing too.
The Client Support
One downside of Podia is the lack of phone support. You have the option to email Podia or go through the 24/7 live chat area. The Shaker plan even gets onboarding and migration assistance if you plan on moving from another platform.
Along with a site, documentation and a Resources page, Podia support is about as good as it could get without phone assistance.
Your Podia checkout is protected by Stripe and PayPal. Podia does not collect any information when one of your customers goes through the checkout, and also the checkout pages are completely secure. The transactions are routed through an encryption process and shielded from 128-bit SSL encryption technologies.
I would like to see some fraud protection, since Membership and online course sites often see people trying to video game the system.
One advantage is that both pricing plans have SSL Certificates for locking down transaction security.
Application Procedure
Establishing a Podia account is straightforward and mostly surprise-free. You are going to need to connect to some Stripe (see our review) account to enable memberships, however.
Project Qualifications
Should you set up a Podia account, you will be able to start Selling memberships, electronic courses, and electronic downloads once you complete the account setup procedure. Podia's terms of service prohibit copyright infringement, misleading/fraudulent content, spamming, and libel.
The TOS also lay out a policy contrary to Substance that"includes or depicts nudity, comprises or depicts sexual activity, or is otherwise inappropriate as determined by us at our sole discretion" This might seem to preclude all articles that could be considered"adult." This is somewhat curious, considering Podia promotes itself as a more creator-attuned alternative to Patreon, which will allow some forms of adult content. At least in this regard, Podia is more restrictive of creators than Patreon, not less.
Customer Service &Technical Support
Podia Provides a Series of articles that will help you through different aspects of working with the service. Concerning direct assistance, Podia offers email service and live chat. In reality, Podia's creator offered to match with me at the NYC area once I signed up for the ceremony!
Sales & Advertising Transparency
Podia Discloses relevant information regarding its services on its own website, and the data looks comprehensive and complete. Sell My Courses Online
Favorable Reviews & Testimonials
The Aforementioned video review of Podia was fairly favorable general, praising the layout, aesthetics, and community-building features of this platform.
Negative Reviews & Complaints
I couldn't find much in the way of feedback regarding Podia. One of the only reviews I could find online — a video review posted to YouTube — noticed that unlike some rival platforms, Podia does not allow for multiple authors within a single account. Another review noted that the system"is not the cheapest solution on the market."
As Previously Mentioned, the Podia system provides a 30-day Free trial without the need to type on your credit card information. What is more is that in the event that you upgrade to a real plan within five days of starting your free trial, a 15% lifetime reduction is thrown onto your own plan.
Annual and monthly billing plans are revealed, so naturally, In the event you pay for Podia on a yearly basis you are likely to find a better monthly fee.
Although Podia isn't the cheapest way to make an Online shop, the pricing isn't bad at all. In addition, it makes up for the cost with all the awesome features and simple user interface.
One thing I like about Podia is that it only includes two Pricing plans. Therefore, you're not confused when trying to determine which characteristics you need to cover.
Here's what's available concerning pricing.
The Mover Plan — $33 a month for all basic Podia features and drip course material.
The Shaker Plan — $67 per month for all basic Podia features, innovative visitors, onboarding, migration support, drip course content, affiliates, and memberships.
All plans have the next.
0% transaction fee to Podia. This doesn't include the fee you will have to cover Stripe or PayPal.
Email newsletters for sending out manual and automatic emails.
Prelaunch product pages.
Endless courses.
Infinite digital downloads.
Drip email content.
Customer tracking.
A high-conversion checkout site.
Many integrations such as ConvertKit and Zapier.
Payouts that instantly go to your own bank account.
Data exporting tools.
A custom domain name to sell items from your own site.
A payment protection program.
Who Should Consider Podia?
I like this app for those who have discovered that Gumroad, or other competitors, are either too intricate or they force you to pay high transaction fees. Podia offers an excellent user experience along with a rapid launch procedure for online courses, digital downloads, and membership websites.
Click here to subscribe to Podia now!


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The Original Williams Sonoma Peppermint Bark, Set of 2
- Only at Williams Sonoma
Easy, hassle-free returns online and in-store for eligible items.Learn More
Earn 2x Rewards4 That's 10% back in rewards when you use your Williams Sonoma Credit Card at Williams Sonoma 12/14 - 12/21.Apply Now Learn More
- Summary
Our nostalgic peppermint bark is often copied but never matched in quality or flavor. The once-a-year favorite is crafted using the finest ingredients, including custom-blended Guittard chocolate and triple-distilled oil of peppermint. This "bark" is finished with a snowfall of peppermint candy pieces. The result is an unforgettable holiday confection with a perfect harmony of flavors and textures in every bite.
- Available in 1- or 2-lb. collectible tins.
- Use & Care
- Store in a cool, dry place.
- Not safe for animals.
- Ingredients
- Sugar, unsweetened chocolate (processed with potassium carbonate), cocoa butter, milk, milk fat (butter), corn syrup, vanilla, sunflower lecithin, peppermint oil, natural red color.
- Contains milk. This product is prepared and packaged using machines that may come in contact with wheat/gluten, soy, tree nuts, peanuts.
- Shipping
This item cannot be shipped to Hawaii but is available for purchase at our Honolulu retail store.
Shipping Information
UPS can deliver most in-stock items within 3–5 business days. UPS is unable to deliver to P.O. boxes.
Shipping Rates
The shipping rate varies depending on your order total and shipping destination. View Shipping Options and Charges.
Next Day Delivery
For an additional charge of $26 per address, we can expedite delivery to most destinations. This service is not available for some oversize items, items shipped directly from the supplier (including perishable and custom-manufactured items), out-of-stock items and items to be shipped outside the contiguous 48 states.
Orders received by 9:00 p.m. ET Monday through Thursday will arrive the next business day; orders placed Friday through Sunday will arrive on the following Tuesday.
Return Policy
At Williams Sonoma, we take great pride in the quality and craftsmanship of our products. Attention to design, materials, safety and construction are our priority. Upon receipt, please inspect your purchase and notify us of any damage; we will arrange for a prompt replacement.
If within 30 days, you are dissatisfied for any reason, you may return your purchase for a refund of the merchandise value. An original receipt or gift receipt is required for all returns and exchanges. Returns with a gift receipt will be refunded in the form of a Merchandise Credit for the amount indicated on the gift receipt. Returns with original receipt will be refunded in the original form of payment, cash and check refunds over $100 will be issued as a company check (may take 14 business days from time of request).
For all cutlery returns (knife sets, single knives, cutting boards, knife sharpeners and knife storage), please contact us at 888.922.4108 to initiate the return.
We cannot accept returns on monogrammed, personalized, special-order items, custom upholstery, food, items shipped direct from the vendor or on items damaged through normal wear and tear. Final sale items ending in .97 or .99 cannot be returned.
Made to Order and Final Sale Furniture items are non-returnable and cannot be cancelled once the order is placed.
For returns of items purchased from your Williams Sonoma Gift Registry, we will gladly provide a refund or exchange for the merchandise within 90 days of your event or within 90 days of purchase, whichever date is later. View Full Returns Policy.
Also in this Collection
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Williams Sonoma Handcrafted Toffee
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Williams Sonoma Chocolate Sea Salt Caramels
Sea Salt Caramels
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Rated 1 out of 5 by Candy from Mediocre There just isn't enough peppermint pieces and there is better chocolate out there. There should also be an option without the expensive to.Date published: 2019-12-07Rated 1 out of 5 by PumpkinPeppermint from Misleading and deceitful It is NOT "the original" peppermint bark, they changed the recipe and ingredients. It has sunflower lecithin in it now, I'm deathly allergic and can no longer enjoy this bark. It has been a favorite since childhood and the holidays are not the same. Please change back to the TRUE original peppermint bark!Date published: 2019-10-22Rated 1 out of 5 by Elaine from Huge change in product--very disappointed! I was so looking forward to receiving my Peppermint Bark, and it arrived today. This is WAY different than it used to be--the quality has gone way downhill. The bark is VERY thin, and when you break it the white chocolate layer completely separates from the dark chocolate layer. And there are VERY FEW peppermint candy pieces on top (and they were chewy and not crunchy like in the past!)--most pieces didn't have ANY peppermint candy on them! The peppermint flavoring is also VERY light compared to past years. I'm surprised at the huge difference--I miss the thick pieces that were really hard to even break apart, and the abundance of crunchy candy--please go back to your original recipe!Date published: 2019-01-28Rated 2 out of 5 by Alycia from Stale Peppermint Chips! For years and years my family has looooved this bark. This year the tin contained old peppermint, or maybe it was exposed to heat in the factory. The peppermint was completely stale and chewy and made the bark very unenjoyable. I was going to bring one to a work function but decided against it after tasting another box (it was a double order so I assumed same batch). Very disappointing for the price. Two stars... one per layer of delicious chocolate.Date published: 2019-01-18Rated 5 out of 5 by Ziggy MD from My favorite Holiday treat to give and receive. I had to write this review after reading some of the lesser reviews of my favorite Holiday tradition William Sonoma Peppermint Bark. Over the last decade I have spent on average of two hundred dollars every year for family and friends on Peppermint Bark and not once has the quality and consistency of this Delicious treat ever changed. My children believe Santa's elves make it therefore it will continue to be in their stockings every year.Again just wondering after such a long history with my purchases how can there be anything negative except maybe you ate too much! P.S. Love Love the sea salt well done.Date published: 2019-01-11Rated 5 out of 5 by Joe Joe from Can we get it all year????????????? I bought 4 as gifts for family and decided to bring some to work to share. The team loved it and I had to buy 3 more packages...No worries glad to be the star especially with such a fabulous product. Keep it up the best recipe around. Well worth the wait.Date published: 2019-01-10Rated 5 out of 5 by KeenanT from Authentic I've tried other suggested retailers, but the quality doesn't compare. Williams Sonoma bark is Hallmark traditional. The lack of uniformity is what gives it the authentic "handmade" feel. Break it up, place it in a bowl, and it's a hit at any holiday festivity.Date published: 2019-01-10Rated 5 out of 5 by Rodi from Big Hit I just shared this at a New Year's Day party and it was a huge hit with everybody. People loved the flavor and texture and it was gone by the end of the party. Several guests wanted to know where to purchase it. It was delicious.Date published: 2019-01-08
I bought 1lb Original Peppermint bark on April 20, 2019 at the Maine Mall and noticed the best by date as best before April 2019 when I got home. Sales Manager said he could do nothing, that per FDA they should sell for another year! What is the story?Hi Susieq, The best by date is not an expiration date. It is just a recommended sell by date to ensure 100% flavor, texture, and overall best product experience. The product is not expired though, and it is perfectly safe to eat. Williams Sonoma continues to sell product for a year past its best by date.Date published: 2019-12-03
What is the 20th anniversary in reference to?Hi PanicMan, 20th anniversary is in reference to when we first introduced our Peppermint Bark.Date published: 2019-01-28
What is the expiration date of the units that are now discounted? ThanksHi br5874, Shelf life on the peppermint bark is a year. Expiration date would be July 2019, at the earliest.Date published: 2019-02-18
Is the Original Peppermint Bark kosher? There's no label/symbol on the outside of the tin.Hi Anonymous, Our Original Peppermint Bark is not kosher.Date published: 2019-01-20
Over the years I've accumulated a LOT of these tins. Do you perhaps have a refill program? Can I recycle the tins at one of your stores? I do reuse them for cookies and crafts, but it seems like getting my old tin refilled each year would be much better!Hi SKH28, Unfortunately, we do not have a refill or recycle program for our Peppermint Bark tins.Date published: 2019-02-01
Is there a way I can order the same item and ship to 6 different addresses or do I need to do them all separately?


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Synthesis of crystals with a programmable kinetic barrier to nucleation
Edited by Alan R. Fersht, University of Cambridge, Cambridge, United Kingdom, and approved July 19, 2007 (received for review February 16, 2007)
A central goal of chemistry is to fabricate supramolecular structures of defined function and composition. In biology, control of supramolecular synthesis is often achieved through precise control over nucleation and growth processes: A seed molecule initiates growth of a structure, but this growth is kinetically inhibited in the seed's absence. Here we show how such control can be systematically designed into self-assembling structures made of DNA tiles. These structures, "zig-zag ribbons," are designed to have a fixed width but can grow arbitrarily long. Under slightly supersaturated conditions, theory predicts that elongation is always favorable but that nucleation rates decrease exponentially with increasing width. We confirm experimentally that although ribbons of different widths have similar thermodynamics, nucleation rates decrease for wider ribbons. It is therefore possible to program the nucleation rate by choosing a ribbon width. The presence of a seed molecule, a stabilized version of the presumed critical nucleus, removes the kinetic barrier to nucleation of a ribbon. Thus, we demonstrate the ability to grow supramolecular structures from rationally designed seeds, while suppressing spurious nucleation. Control over DNA tile nucleation allows for proper initiation of algorithmic crystal growth, which could lead to the high-yield synthesis of micrometer-scale structures with complex programmed features. More generally, this work shows how a self-assembly subroutine can be initiated.
Biology demonstrates how self-assembly can create sophisticated organization on the molecular scale. The fundamental challenge in engineering a self-assembled object is that its molecular components must themselves contain the information needed to guide self-assembly.
Controlling the nucleation of a self-assembled object is the first step in controlling the self-assembly process, as exemplified by the formation of actin networks (1, 2), the growth of microtubules on the centrosome (3), and the assembly of bacterial flagella (4). These systems avoid the difficulty of controlling homogeneous (unseeded) nucleation by relying on heterogeneous (seeded) nucleation: Growth is rare except in the presence of a seed molecule, from which it proceeds with little or no kinetic barrier. Here we demonstrate a general strategy for designing self-assembled molecular structures whose nucleation is controlled by a seed. We use programmable DNA tiles to create a series of seeded "zig-zag ribbons" that have increasing kinetic barriers to homogeneous nucleation.
DNA tiles (5, 6) are a general-purpose nanoscale construction material. A DNA tile consists of multiple, interwoven strands that form double helices connected by "crossover points" (Fig. 1 a). Tiles bind to each other when their single-stranded ("sticky") ends hybridize (Fig. 1 b) and can assemble into extended structures, including one- and two-dimensional crystals (7–10). Tile interactions are programmed by design of tile sticky ends: Complementary sticky ends hybridize, whereas noncomplementary sticky ends are unlikely to interact. Under slightly supersaturated conditions, where the attachment of a tile to a crystal by two or more sticky ends is favorable but attachment by just one sticky end is unfavorable, in principle it is possible to program complex assembly processes (11).
We have constructed sets of DNA tiles that form ribbons of particular widths. Ribbon assembly proceeds in two phases: nucleation and growth. Under slightly supersaturated conditions, homogeneous nucleation requires both favorable and unfavorable tile attachments. In contrast, growth requires only favorable monomer tile addition reactions and proceeds quickly at both ends of a ribbon along a zig-zag path (Fig. 1 c and d). Design of nucleation and growth pathways is possible because, under slightly supersaturated conditions, attachment by two (or more) sticky end bonds is favorable, whereas attachment by a single bond is unfavorable. This makes it possible to design the height of the barrier to homogeneous nucleation: The number of unfavorable attachments required for nucleation is the width of a ribbon (in tiles) minus 1. Theoretically, increasing the number of required unfavorable reactions can exponentially reduce the rate of nucleation (12).
In this article, we describe the design and synthesis of ribbons that are three, four, five, and six tiles wide, which are denoted ZZ3–ZZ6. Each ribbon forms as designed and has a significant kinetic barrier to homogeneous nucleation. Measurements of ribbon growth show that nucleation rates are lower for wider ribbons under slightly supersaturated conditions. We then synthesize a seed molecule for ZZ4 ribbons and show that the kinetic barrier to nucleation is greatly reduced in its presence.
Results and Discussion
Tile and Ribbon Structure.
Tiles were designed and characterized according to established protocols [see supporting information (SI) Appendix S1 ]. To verify that the tiles assembled into ribbons, the component strands of each ribbon were mixed together at 100 nM (per strand) and annealed from 90° to 20°C over 20 h in a PCR machine. Atomic force microscopy (AFM) of each sample predominantly showed the desired structures (Fig. 2). Most ribbons were micrometers (hundreds of tile layers) long, suggesting that the formation of new ribbons (nucleation) was much slower than the growth of existing ribbons. To establish that annealing is necessary to produce long ribbons, we mixed preformed ZZ4 tiles at room temperature, at which nucleation is presumed to be fast, and let them sit for 20 h. Few ribbons longer than 10 tile layers were observed (see SI Appendix S2 ).
Homogeneous Nucleation Rates.
We used 260-nm spectroscopy to measure the rate and temperature dependence of zig-zag ribbon growth and melting. Single-stranded DNA has a higher absorbance at 260 nm than does double-stranded DNA (hyperchromicity), so measured absorbance is proportional to the amount of unpaired DNA and slightly dependent on temperature (13). We recorded the absorbance of a ZZ4 sample at 50 nM as it was annealed from 90° to 15°C, held for 2 h, and melted back to 90°C at 0.13°C per minute (Fig. 3 a). The resulting plot had two transitions: a reversible change between 70° and 45°C and a hysteretic transition between 40° and 15°C. Annealing and melting 50 nM of ZZ4 tiles without sticky ends (which cannot form ribbons) produced only the high-temperature, reversible transition, suggesting that tile formation and melting produced the reversible transition, whereas ribbon formation and melting produced the hysteretic transition. This interpretation is consistent with previously measured melting temperatures of DNA tiles and tile assemblies (14). We repeated this "temperature-ramp" experiment with ZZ3–ZZ6 at 25, 50, 100, and 200 nM (see SI Fig. S3).
Hysteresis signifies a kinetic barrier to nucleation. During the anneal of ZZ4, the solution became supersaturated (i.e., crystal formation became favorable) at ≈37°C (see below), but nucleation was not discernible for another 75 min, when the temperature had decreased to 27°C. To study how the amount of hysteresis changed with ribbon width and concentration, we compared absorbance traces produced by ribbons of different widths and concentrations (Fig. 3 b and c). The traces were normalized and the absorbance due to tile formation was subtracted, so that the onsets of formation and melting could be more clearly seen. We defined the formation temperature, T f, as the first temperature at which the normalized absorbance reached 0.8, i.e., where significant growth is first observed. T f decreased with concentration, but only slightly with ribbon width (Fig. 3 d). The melting temperature, T m, defined as the point at which the normalized absorbance equals 0.5 (i.e., the point at which half the ribbons are melted), did not change appreciably with width or concentration.
To determine whether these results were compatible with the designed pathways of zig-zag growth and nucleation, we constructed a simplified model of ribbon growth and nucleation, which we call the "standard sequence model." This model (Fig. 1 d) considers a single prototypical assembly, An , containing n single or double tiles, thus ignoring the combinatorial number of possible species of each size. The model includes all reactions of the form for 1 ≤ n ≤ m, such that n + m ≤ M, where M is the largest ribbon size modeled. M was limited by computation time to 100. The included reactions allow growth by monomer addition, as well as joining and internal scission of ribbons (15). To approximate the size dependence of the forward rate constant, we used k f n,m = kf = 106 M−1·s−1 [typical for oligonucleotide hybridization (16)] for reactions involving a single tile or unnucleated assembly and k f n,m = k j = 35,000 M−1·s−1 for two ribbons joining, which we measured experimentally (see SI Appendix S3 ). Thermodynamics determined the reverse rates: k r n,m = k f n,m exp{−(ΔG n° + ΔG m° − ΔG n+m°)/RT}. An has standard free energy ΔG n° = bn ΔG se°, where bn is the number of sticky end bonds in An (e.g., b 6 = 7 for the top assembly in Fig. 1 d) and ΔG se° is the free energy of hybridization for a single sticky end. We estimated or extrapolated these parameters from measured values. Assuming that the energetics for binding by multiple sticky ends are additive, we used ΔG se° = ½(ΔH° − T ΔS°) where ΔH° and ΔS° are the enthalpy and entropy of a tile attaching to a ribbon by two sticky ends, as measured experimentally below.
The standard sequence simulations qualitatively reproduced most of the features of the absorbance traces (see SI Fig. S6), including the strong dependence of T f on concentration and the steeper slope of the annealing curves for higher concentrations. The model also reproduced the lack of dependence of T m on ribbon width but predicted an ≈3°C difference between T m values at the highest and lowest concentrations, as would be expected from thermodynamics. This difference was not observed in experiments. At each concentration, both simulations and experiments showed similar T f values for ZZ4–ZZ6 and a T f for ZZ3 that was only slightly higher, even though the model assumed a different critical nucleus size for each ribbon width. This finding suggests that T m − T f is not a measure of the height of the nucleation barrier under slight supersaturation. Rather, Tf in the temperature-ramp experiments is the temperature at which the nucleation barrier becomes insignificant (i.e., the solution becomes highly supersaturated).
Theoretical results (12), however, predict that nucleation rates decrease with ribbon width only under slightly supersaturated conditions, for which the critical size for nucleation is largest. Measuring nucleation rates under these conditions is challenging: Predicted nucleation rates are minuscule and exquisitely sensitive to both temperature and tile concentration. In two- or three-dimensional crystal growth, the rate of monomer depletion increases quickly after nucleation because the number of growth sites on a crystal increases as it grows. In contrast, a zig-zag ribbon has only two growth sites, regardless of its length, so the nucleation transition is not sharp. Furthermore, nucleation, growth, and ribbon joining (15) may occur simultaneously and on the same time scale, so that the absorbance changes resulting from continued formation of tiles and other nonidealities can be of the same magnitude as the absorbance changes that result from nucleation and growth.
These difficulties suggested that observation of ribbon growth at slightly supersaturated conditions over long periods of time would be necessary to observe differences in ribbon nucleation rates. So, for a range of target temperatures, T, we annealed the ribbons from 90°C to T and watched them grow (and approach equilibrium) for 24 h at that temperature. To determine the absorbance that corresponded to the equilibrium state at that temperature, we then annealed the ribbons to 15°C, held them there for 2 h, and then melted them back to the target temperature, where they were held for another 24 h. All temperature changes were made at the same speed as in the temperature-ramp experiments.
For these "temperature-hold" experiments, we held samples of ZZ3, ZZ4, and ZZ6 at target temperatures between 25° and 41°C. Absorbance traces from three ZZ4 experiments are shown in Fig. 4 a–c. At 39°C, above the melting temperature, there is no activity apart from an initial transient attributed to tile formation. At 33°C, there is a large barrier to nucleation: After 24 h, a significant separation still exists between the anneal and melt. At 25°C, there is a smaller kinetic barrier to nucleation: The absorbances of the anneal and melt holds converge within 24 h. The traces of all melt holds show no significant change after 6–12 h.
To study growth rates, the normalized absorbances at the beginning and end of each hold were plotted, as shown in Fig. 4 d–i (for 50 nM and 200 nM, see SI Fig. S7). Above 35–37°C, absorbance at the end of anneal and melt holds converged; at these temperatures no ribbons formed. At lower temperatures, the hysteresis remaining after 24 h (the gray areas in Fig. 4 d–i) increased with increasing ribbon width and decreasing concentration (see SI Fig. S8). The increase in hysteresis was a result of decreased formation temperatures; melting temperatures exhibited no measurable dependence on ribbon width and only a small dependence on concentration. (T f and T m for temperature-hold experiments were defined analogously as for temperature-ramp experiments. The values are different for the two types of experiments because they are kinetic, not equilibrium, measurements.) The low formation temperatures of wider ribbons means that seeded growth of wider ribbons can proceed over a wide temperature range without significant spontaneous nucleation.
Standard sequence simulations qualitatively reproduced the measured hysteresis, and the formation and melting temperatures, as a function of ribbon width and concentration (see SI Fig. S9). However, hysteresis was consistently more pronounced in simulation. As predicted by the model, ribbons at higher concentrations melted at higher temperatures. Equilibrium tile concentrations were achieved within a few hours of holding during the melt in the simulations. Because absorbance values also quickly reached their maximum values in all of the melt hold experiments, we inferred that equilibrium was achieved there as well.
We used the dependence of T m on concentration to estimate the energetics of tile attachment. At T m, the free energy of tile attachment, ΔG = ΔG° − nRT ln([m]) is 0, where ΔG° = ΔH° − T ΔS° is the standard free energy of formation for a tile attaching by two sticky ends and [m] is the concentration of unbound tiles of each type. We fit ΔH° and ΔS° by using a van't Hoff plot (17) of 1/T m vs. R ln([m]0/2), where [m]0 is the initial tile concentration (Fig. 4 k). The slope and intercept of this plot are ΔH° and ΔS°, respectively. Because the melting temperatures were not dependent on ribbon width, we pooled them for fitting and discarded the outlier (ZZ3, 100 nM). We measured ΔH° = −102.4 kcal/mol and ΔS° = −0.300 kcal/mol/K, which are comparable to average values (−99.7 kcal/mol and −0.276 kcal/mol/K) predicted by the nearest neighbor model of DNA hybridization (18, 19). At 37°C, our measurements (analyzed by bootstrapping) give ΔG° = −9.43 ± 0.21 kcal/mol.
Accurate estimates of nucleation rates are difficult to obtain, as mentioned earlier, because zig-zag ribbons have temperature- and concentration-dependent critical nucleus sizes, and nucleation, growth, and joining happen on similar time scales. Nucleation rates therefore cannot be treated uniformly by classical nucleation theory (20). We instead used the temperature-hold results to directly estimate nucleation rates under conditions in which the tile concentration [m] decreases only marginally, and thus the nucleation rate n r remains approximately constant. We used where [m] is the concentration of each tile type, [r] is the concentration of ribbons ([r] = 0 at t = 0), k f and k r = k f e ΔG°/RT are the rate constants for tile attachment and dissociation, and N = 2w − 2 is the number of tile types for a ribbon of width w. We used k f = 106 M−1·s−1 and k j = 35,000 M−1·s−1, as in the standard sequence model. [The significant joining rate explains why anneal holds in the hysteretic regime (e.g., Fig. 4 b) stopped changing before reaching equilibrium: The nucleation rate was reduced because of its dependence on tile concentration, and few ribbon ends remained to deplete tile concentration.] To find n r, we solved these equations using [m] values at t = 0 and 24 h after annealing (see SI Appendix S4 ). We estimated [m] linearly from absorbance by assuming that the dashed line corresponded to [m]0 and that [m] was at equilibrium at the end of the 24-h melt holds.
The nucleation rates at the melting temperature [where the supersaturation σ = ln([m]0/[m] eq ) = ln(2) is mild] are 3 × 10−7 nM/s, 10 × 10−7 nM/s, and >70 × 10−7 nM/s for ZZ6, ZZ4, and ZZ3 at 200 nM, respectively. Uncertainties from measurement error, estimation of k f and k j, and residual absorbance change due to continued tile formation render absolute values for n r unreliable. However, for every concentration except 25 nM, the inferred nucleation rates decrease monotonically for wider ribbons, and this conclusion is robust to 10-fold changes in k f and k j. Although the measured decrease is not as strong as predicted, these results support theoretical predictions that nucleation rates should decrease with width (12).
Heterogeneous Nucleation of Ribbons.
To test whether a segment of full-width ribbon could seed ribbon growth and remove the barrier to nucleation, we designed a seed for ZZ4 resembling two tile layers of the ribbon. The seed has the same sticky ends as ZZ4 on its right side (Fig. 1 c), but its strands are woven so that it cannot easily fall apart into individual tiles (Fig. 5 a). The seeds formed with ≈50% yield (Fig. 5 b). They have a melting temperature of 62°C at the concentration at which the seeds were used, 2 nM (see SI Fig. S10), well above the melting temperatures of the ribbons.
To demonstrate seeded growth, two samples of ZZ4 were annealed from 90° to 40°C, at which point seeds were added to one of the samples. The samples were then cooled from 40° to 34°C and held at 34°C for 12 h. After the hold, the samples were cooled to 15°C and reheated to 34°C, and the temperature was held for another 12 h (Fig. 5 d). By the onset of the hold, the distance between the anneal and melt absorbances was already smaller in the sample to which seeds were added. We propose that ribbons had by this time already nucleated on the seeds. The difference in the anneal and melt signals disappeared completely after a couple of hours for the seeded sample. Growth remained slow in the seedless sample.
The results here indicate that it is possible to engineer pathways of crystal growth by using rational design. More generally, our methods suggest a way to control the onset of a stage of self-assembly. Used recursively, this control can allow the ordered progress of multiple self-assembly reactions that together produce a complex structure.
Such kinetic control within self-assembly can be surprisingly powerful. It is theoretically possible to design a set of DNA tiles to assemble any computable shape or pattern (11, 21). This algorithmic crystal growth has been demonstrated experimentally (14, 22), but thus far spurious nucleation and errors during growth have resulted in poor yields. It may be possible to use the techniques developed here to reliably grow technologically relevant molecular structures with high yield, including crystals of exact rectangular dimension (23, 24) and the layout for a nanoscale demultiplexer circuit or memory circuit (25). Further, the control of nucleation in ribbons could allow single-molecule detection or the replication and evolution of crystal sequences encoded in ribbons (26).
The approach described here for control over nucleation is potentially applicable to other organic and macromolecular crystals if sufficient control over intermolecular interactions can be achieved by design. Generalization to two- or three-dimensional crystal growth is also possible (27, 28). Control over nucleation of supramolecular assemblies can also be achieved by conformational changes (29) or by energy-consuming enzymatic activity (30). These methods, along with the work described here, present the engineer of complex self-assembly processes with a rich design space.
Materials and Methods
DAO-E tiles (Fig. 1 a and b) were used to construct ribbons (7). Sequences for the double-stranded tile regions were either those used previously (14) or those designed by computer using sequence symmetry minimization (31, 32), a technique that selects sequences with minimal undesired intramolecular and intermolecular interactions. Sticky end sequences were designed so that binding strengths between pairs of complementary ends were similar and binding energies between noncomplementary ends were minimized. Binding energies were estimated by using the nearest neighbor model (17).
Reactions were performed in Tris-Acetate EDTA buffer containing 12.5 mM hydrous Mg(CH3COO)2. Nondenaturing gel electrophoresis showed that all tiles formed with at least 80% yield, and crystal seeds formed with ≈50% yield. Kinetics and temperature-dependent measurements of UV absorbance were performed using an AVIV 14DS spectrophotometer (AVIV Biomedical, Lakewood, NJ) equipped with a computer-controlled temperature bath. AFM was performed on a Digital Instruments Nanoscope III (Veeco Metrology, Santa Barbara, CA) in fluid tapping mode, using NP-S tips. Samples in Fig. 2 were prepared by pipetting 40 μl of buffer followed by 3 μl of sample onto freshly cleaved mica. The sample in Fig. 5 b used buffer containing 5 mM NiCl2 instead of Mg(CH3COO)2.
Design and experimental details are given in SI Appendix S1 .
We thank Paul Rothemund, Bernie Yurke, Peng Yin, Ho-Lin Chen, Rizal Hariadi, Rick Flagan, and Rob Barish for insightful discussions and encouragement. This work was supported by National Aeronautics and Space Administration Astrobiology Grant NNG06GA50G and National Science Foundation Grant NANO/CCF-0432193.
- ‡To whom correspondence should be addressed. E-mail: winfree{at}dna.caltech.edu
Author contributions: R.S. and E.W. designed research; R.S. performed research; R.S. and E.W. analyzed data; and R.S. and E.W. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/cgi/content/full/0701467104/DC1.
- Abbreviation:
- atomic force microscopy.
Freely available online through the PNAS open access option.
- © 2007 by The National Academy of Sciences of the USA
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NZ4736 Air New Zealand - Arrival Toronto Pearson Airport (YYZ)
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Kim Kardashian has shrugged off the humiliation that she and Kendall Jenner received at the Emmy Awards, as she shared an adorable snap of daughter Chicago.
The reality star took to Instagram to post a cute photo of one-year-old Chicago in an all-pink tracksuit, paired with white trainers, with her hair up in bunches.
'My Chi Chi,' Kim captioned the post.
Kim is mum to daughters North and Chicago and sons Saint and Psalm with husband Kanye West.
This comes just after the 38-year-old and her half-sister, Kendall, were laughed at by the audience at the Emmy Awards the previous night (22 September).
The two stars took to the stage at the Microsoft Theatre in Los Angeles to the present the award for Outstanding Competition Series, but did not receive the most welcome reaction from those in attendance, as they prepared to give a speech about 'truly compelling television'.
The audience erupted into laughter, as Kim declared: 'Our family knows first-hand, how truly compelling television comes from real people just being themselves.'
'Telling stories unfiltered and unscripted,' Kendall added.
The laughter continued, as many people – both in attendance and viewers at home – took note of Kim referring to her family as 'real people'.
The supposed irony was not lost on people tuning in, as people called the audience reaction 'cringe' on Twitter.
Kim later opened the envelope to reveal that RuPaul's Drag Race had taken the gong for Outstanding Competition Series, beating the likes of The Amazing Race, The Voice and Project Runway to snag the gong.
Got a showbiz story?
If you've got a celebrity story, video or pictures get in touch with the Metro.co.uk entertainment team by emailing us firstname.lastname@example.com, calling 020 3615 2145 or by visiting our Submit Stuff page - we'd love to hear from you.
MORE: Kim Kardashian strutted around Tupac Shakur music video in bikini at 14 after lying about age
MORE: Kim Kardashian loves being in Wyoming because she can wear no make-up and her sweat pants: 'It's just so chill'


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(comparative more empirically, superlative most empirically)
- Based on experience as opposed to theoretical knowledge.
- I have no idea why this works, but empirically, it works.
- (sciences) Based on data gathered in the real world.
- We need to test this idea empirically instead of just speculating about it.
empirical + -ly


text:
Skip to this video now. Loni Bouchard arrested in Connecticut for allegedly having sex with boy, Here's how to inoculate ourselves against negative ones.
Carrie Sarah Jessica Parker and Mr. More sex please, we're post-millenials: Here come the girls For many young women, the sexual rhetoric is simple. "A slut is . lad culture is – for many women – the underlying motive behind the sexual double standard. But Carine Diebolt, the lawyer for the family, asked the court on Tuesday to re-characterise the charge from sexual abuse to rape.
One or two of them were squinting and compromising, looking at their appearance, and few were happy. A Kansas judge says that two teenages girls are partly to blame for being solicited to have sex with a year-old man. I went to a nice school.
Once a week, Daily Intel takes a peek behind doors left slightly ajar. Yesterday did not go as planned. Met up for date at the Soho Playhouse but had fight after the show. It ended with me crying and him deciding to go home solo.
Skip to content. A boy who was the leader of the Young Conservatives Club asked, "But what if it's just The girl, who'd had sex twice in 11th grade with a boy she later His attire every day for the two weeks I attended the class in February. Arrayed before Vernacchio was a circle of small desks occupied by 22 teenagers, six male and the rest female — a blur of sweatshirts and Ugg boots and form-fitting leggings.
It's not something that happens to nice girls. I'd had a drink while I was getting ready with friends and two cane and creme sodas later at the.
The Young Turks a year-old Lincoln math teacher who pleaded not guilty Friday to two counts of third-degree child rape and one count of.
21 hours ago Two women walk with their hands clasped together as they're Related: Trafficking of young girls in India a growing problem amid gender imbalance. Marco Werman: Have you seen sex traffickers posing as modeling scouts.


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Thanks to our restaurants,
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for a very successful 2019 Taste of the Town!
- Savor menu favorites of more than 20 popular area restaurants.
- Dance to the beat of "The Answer To Classic Rock" band.
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The Chronogram: Oct. 29, 2018
We all know the feeling. You're an hour into your drive back to the city after a relaxing weekend upstate when that doubt starts to seep in. "I think I locked the sliding door to the deck. Yes, I am sure I did," you try to convince yourself. Minutes later, the doubt settles in and you're turning around and heading back to make sure your house is secure, adding hours to your trip and removing that refreshed, carefree feeling you were so enjoying.
Read more in The Chronogram.


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Hi ppl
There is a latency in audio in a online meeting, not in the st client. Even if i run "test a meeting"
Can you set the sametime server to not buffer the audio?
I have tested changing the
"Audio/Video jitter buffer" to 10 or 100 resulted in no particulary improvement.
Audio frames per packet=1, tried to have blank then it didnt work at all.
tried changing the "Type of Connection Speed Setting" still delay
As i understand you only have 3 options for sound perfomance?
The network or the server hardware is no issue, and the sound in the sametime client is great. So there is something in the meeting. Is there any unsupported tricks we can try?
Please give me a tip


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Have you yourself experienced burnout? Have you seen burnout in a peer?
John Fezza, MD: Everybody experiences some degree of burnout at some point. This is not exclusive to medicine, but certainly can be more prevalent due to the higher stress of patient care. Fortunately, aesthetic medicine is evolving so rapidly that boredom is really not a factor. The plethora of new ideas and innovations keep our field uniquely interesting.
Burnout can come from long hours and nights, which limits personal free time. I find an occasional vacation to recalibrate helps to keep me fresh. Also exercise and good nutrition help keep me upbeat and on top of my game.
Sabrina Fabi, MD: I have moments were I may need a vacation, but I think that's true regardless of the field one is in. Although lofty, my goal is to come every day to work feeling passionate and excited, and I think it comes down to always being clear on my "why." I would say that I am aware that physician burnout is an epidemic and so I try to stay focused on why I love what I do (which can get blurred when dealing with charts, billing, insurance companies, employees, HR issues, etc.).
I try to make sure that most of my time is focused on doing what I love and try to delegate what takes away from that time. My passion is treating and connecting with patients and teaching and connecting with residents and colleagues, and not all the administrative tasks that sometimes take away from this. So at our practice each of us have three medical assistants so we can spend more time doing what we love, which in turn makes us happier and more efficient, and probably prevents physician burnout to a degree.
Adam Schaffner, MD: I have not personally experienced burnout or seen burnout in a peer. Many of us have seen peers whose personal and professional lives have been challenged by substance abuse, bitter conflict, and even illegal activity resulting in criminal or civil action. Burnout may, in part, be to blame for some of these downturns. I believe more than complete burnout, many of us struggle to meet all of the challenges we face on a daily basis. We have more patients we want to treat and more commitments we desire to fulfill than time permits in a given day. I believe it is critically important to learn to say "No." When appropriate, doing so allows one to focus energy on those things that absolutely must get accomplished on a given day and still have quality time to spend with family.
Jason Emer, MD: My focus is strictly cosmetic and there is not a day that goes by that I do not go home and think of a treatment that I performed that day on a patient or the results from my surgeries the week before. It's constantly on my mind. The cosmetic industry is becoming more of a customer service oriented business, similar to a hotel or restaurant, and not only do you have to be top of your game with regards to innovation, technical skill, and medical knowledge but you must also provide the best service and concierge care to keep patients satisfied and keep your reputation (both locally and online) honest and representative. It's not as simple as going to work and performing your duties and ending the day anymore. You have to be always thinking ahead and outperforming your best and that is a lot of stress. There are times where I would rather be on vacation, but then I think about my hard work and dedication over the years and appreciate how lucky I am to be doing what I love everday.
Jose Raul Montes, MD: I have not experienced burnout. I have not seen burnout in a peer either.
What is the biggest contributor to burnout in your opinion?
Dr. Schaffner: Burnout is caused by many different factors including, but not limited to, the number of hours worked per day, inadequate sleep, inadequate exercise, suboptimal diet, and inadequate time away from work to rejuvenate. During a long workday, the intensity of the work, managing employees, dealing with demanding patients and completing a myriad of administrative tasks can cause both emotional and physical exhaustion.
Dr. Fezza: The biggest contributor to burnout is increased regulation. More stringent rules combined with decreasing reimbursements are a large factor in burnout.
Dr. Montes: I believe the biggest contributor to burnout is patient load and working at the office too many consecutive hours and days without taking any time off.
Dr. Emer: For those who do medical dermatology I think it's medical billing, insurance companies, and medical documentation. For my high-end cosmetic practice, it is completely customer service. We have to provide a very high level of concierge care and have to live up to very high levels of expectations with demanding patients…It is a significant undertaking to be successful and keep all staff (and yourself) happy.
Dr. Fabi: I think unnecessary rules/regulations that take away from connecting and caring for patients, which is the main reason many of us went into medicine to begin with.
What do you do for yourself to help avoid burnout and maintain balance?
Dr. Fabi: I think "balance" is like a unicorn. Everyone talks about it but no one has ever seen it. Probably because it is dynamic, different for everyone, and based on individual values/priorities and what brings people personal joy. Stressing one's self to keep "balanced" is probably as stressful and unenjoyable as feeling burned out.
I recognize I am a better doctor and happier when I walk into work when I also engage in the other things that bring me personal joy, which are traveling, spending time with loved ones, meditating, reading, and working out. I think this is different for everyone, and probably changes with time.
Dr. Schaffner: To minimize the risk of burnout, optimize diet, exercise and sleep. Schedule days out of the office to catch up and rejuvenate. Patients expect you to be at 100 percent each and every time you see and treat them. It is impossible to give them 100 percent if you have not given yourself 100 percent of what you need to be at your best.
Dr. Montes: As a priority, I personally work out every other day at the gym. Regular physical exercise is fundamental to keep me energetic and enthusiastic with my daily duties as a busy cosmetic practitioner. Daily meditation and silence for 5-10 minutes helps me stay focused and calm during a busy day.
Dr. Fezza: Keeping a good attitude and surrounding myself with positive like-minded staff and individuals helps avoid burnout. Also taking the time to smell fresh air and stay in shape really helps. Lastly, realizing I am part of an amazing field of medicine that is incredibly diverse and exciting keeps me interested in my work and avoids boredom and burn out.
Compared to when you first started practice, how would you describe your outlook regarding your work: improved, same, or worse? Why?
Dr. Montes: My practice has been in a steady growth and evolution over the past 20 years. I am always studying and searching information on new products and/or procedures to meet my patients' needs. I have learned along the way what makes a practice successful. It is very simple: Put the patient first in your "mathematical" equation and be unique!
At the beginning, I thought that I needed to imitate my "successful colleagues" and compete for the new products or procedures on the market. However, experience has made me realize that I have to be loyal to myself first in order to enjoy the work on daily basis and be successful. I take advantage of my strengths and skills, using my own "style" on everything I deliver to the patients. The whole experience must be excellent in terms service and outcome. The goal is to surprise our patients in the best possible way.
Dr. Fabi: Improved, because I am more self-aware and am clearer on why I love doing what I do, and what it takes to keep focused and not let other things take away from that.
Dr. Fezza: I still love my work as much as the first day I started. In fact my excitement about aesthetic medicine and surgery never slowed. Having been a professional bond trader on Wall Street prior, I did not experience this in my other career. The combination of helping patients along with the technical and artistic components makes it a perfect fit for me.
Dr. Schaffner: I believe the best days lie ahead. I continue to learn from colleagues and through experience, which allows me to deliver better care and obtain better results each day. I believe healthcare will continue to face significant challenges which will require all of us to be as creative and efficient as possible to yield the results we desire.
Dr. Emer: I absolutely love what I do and wouldn't change a single thing. You always have to be yourself and my philosophy has (and always will be) that it's not about the device or the procedure, it's about the philosophy and the physician and the unique approach to treatment that keeps patients coming in.


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In the morning her hair had turned white. She didn't know why. It had been a normal evening, nothing out of the ordinary. Bed at a reasonable time. Nothing had woken her until her normal waking time at 7am.
And yet, her hair had turned white overnight? It was meant to happen if you were scared or had a shock. Something she never understood as there is no blood supply to hair. It's dead, it's colour is created as it grows and as you get older grey or white hair gradually takes over. But overnight? What could have happened?
She had had a shower…… Oh.. …
Peroxide! She had used the wrong shampoo. Her daughter used it normally…. .


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Melissa Ostrom
pubblicato da Feiwel & Friends
**"For fans of Laurie Halse Anderson's Speak..."--**Voices of Youth Advocates
In a book that is both urgent and timely, Melissa Ostrom explores the intricacies of shame and victim-blaming that accompany the aftermath of assault.
After surviving an assault at an off-campus party, nineteen-year-old Maggie is escaping her college town, and, because her reporting the crime has led to the expulsion of some popular athletes, many peoplein particular, the outraged Tigers fansare happy to see her go.
Maggie moves in with her Aunt Wren, a sculptor who lives in an isolated cabin bordered by nothing but woods and water. Maggie wants to forget, heal, and hide, but her aunt's place harbors secrets and situations that complicate the plan. Worse, the trauma Maggie hoped to leave behind has followed her, haunting her in ways she can't control, including flashbacks, insomnia and a sense of panic. Her troubles intensify when she begins to receive messages from another student who has survived a rape on her old campus. Just when Maggie musters the courage to answer her emails, the young woman goes silent.
Generi Non definito
Editore Feiwel & Friends
Formato Ebook con Adobe DRM
Pubblicato 26/03/2019
Lingua Inglese
Scrivi una recensione per "Unleaving"


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What is the most amount of overnight guests you've hosted in your home? If you have thrown a sleepover for one of your kids, then it's not uncommon to have five or six sleeping bags stuffed with the young ones. Typically, most homes can accommodate one or two extra people. Of course, during the holidays all bets are off as families gather to celebrate the season. This could mean expanding your sleeping arrangements to include the sofa in the living room or a cot in the basement. No matter how many people you might have spending the night, you want to make sure your home is presentable. That can happen with one call to Junk King Contra Costa. Although the crews from Junk King Contra Costa won't be able to mop your floors or scrub the bathtub, they can certainly make a huge difference when it comes to clearing out clutter.
You're going to be assigned a two-man moving crew to help facilitate your junk removal process. This Junk King Contra Costa team has been licensed, bonded and insured. That makes them 100% professional and trustworthy. They also have a lot of experience when it comes to lifting all kinds of items from a home. Whether you want to get rid of a piano or sofa, these crews will know exactly how to maneuver those pieces out of the house. If it appears something won't be able to fit out the door before taking apart, then you can count on the crews to bust out the tools and make that happen.
Cost is always a factor especially during the holidays. You'll know the fee you'll be expected to pay before the work starts. That fee will be based on an estimate of how much space your stuff needs on the back of the truck. The only way to get to that final price is for the crew to size up all your stuff in person. Once they present you with an estimate it's a price it's not going to change. Even if your stuff ends up taking up more room you'll still only pay that original price.
Getting your home ready for guests should always start with a call to Junk King Contra Costa. Clearing out the junk will make a huge difference!


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January 2015 News
Happy New Year!
A new year brings new hope, opportunity for growth, and the optimism that the next year will improve upon the last. Thank you for an incredible past year, and here's to an even better 2015 as we build on the progress already made. Please join me as I report to you on the "State of the District" on February 8, 2015. RSVP to attend.
Also, please consider joining your community board. If you are interested in joining a community board, please apply today! As a former community board member,I know that joining the board is a great way to get involved in your neighborhood. Applications close on January 30th.
Whether they be personal desires or wishes for the good of our community and our city, I would like to hear your thoughts about what 2015 holds. What are your New Year's resolutions?
Best wishes to you and your loved ones in the new year,
Ben Kallos
Table of Contents
1. Fight Against the Marine Transfer Station
2. Hail a Yellow Cab on Your Phone
3. Join Your Community Board
4. Bike Safe
5. Commission On Public Information and Communication
6. Early Vote
7. Downsizing Victory
8. Celebrating the Holidays
9. Proclamation for Carol Tweedy
10. Fracking Banned in New York State
11. Honoring the East 79th Street Neighborhood Association for 30 Years of Service
12. Fighting for a World Class Education
13. Local Progress Conference
14. Equality for Women in FDNY
15. Universal Broadband Advocacy Continues with Hearing on Comcast Merger
16. Honored by Labor Press
17. Carl Schurz Literary Landmark
18. Ben in Your Building
19. Mayor's Management Report
20. Free Senior Fitness Class
21. Here to Help
22. Become an Intern
23. In the Neighborhood
24. City Council Events
25. Neighborhood Events


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Robin Biznis May 14 Belgrade, Serbia
Rain has been falling steadily all day, and it has fallen on the last night. The warning says it will fall tomorrow.In the neighboring countries west of Serbia, there is already a flood in the rivers.I mean Croatia and Bosnia and Herzegovina.All this has contributed to the spinach in my garden growing up and ready to take it as soon as it is dry time.Browsing recipes, since I'm a diabetic, I've come across the one I present to you.If someone likes it, they can save it because it's simple.This soup is nice regardless of the seasons, and this is a particularly interesting recipe for lovers of vegetables! It is so rich that it can easily be served as a lighter lunch or a warm evening.Here's what you need:450 gr. of beef (chopped on as small pieces as possible)- 3 garlic cloves- 2 carrots- 1 can of sauce of tomato- 700-800 ml of water or thistle broth- 1 onion- ½ root celery- 1/2 teaspoon of salt- ½ teaspoons of pepper- 1/2 teaspoons of oregano- 150 g of pasta (almonds, shells, spirits)- 200 g of fresh spinachPreparation:For a bit of oil, fry the meat until it gets brownish. Then add the finely chopped white and black onions, then sprinkle them to make a slice.Into this, add finely chopped celery and carrots, stir a bit to add pelat, water / broth, pepper, oregano and so on.Cover and leave to spill, then reduce the fire.Add the dough you like and then cover it again. Pasta will be finished in about ten minutes. In the end, add a large chopped spinach and leave it on the disconnected ring only so that the spinach "shrinks".Serve with sour cream or sprinkled with grated cheese.
Source of photography:http://rs.n1info.com/Cooking/a482316/Juneca-corba-sa-spanacem.html


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Sir Martin Sorrell's new venture S4 Capital takes the challenge to WPP with surging profits and sales
Sir Martin Sorrell's new company continues to step out of the shadow of his old firm WPP, with climbing revenues and surging profits.
S4 Capital, launched shortly after Sorrell ignominiously left WPP, announced that profits are up 40 per cent.
The firm's focus on international clients, particularly American technology giants, appears to be paying off.
Pastures new: Sir Martin Sorrell's S4 capital has been making waves since it's launch
While WPP is the worlds largest group of it's kind both by headcount and revenues, the recently launched S4 is much smaller.
However through deal making and acquisitions, the company now boasts internationally recognized clients.
S4 triumphed against WPP in a high-profile battle to buy Dutch firm MediaMonks, which also recently opened an office in San Francisco.
In an interview with the Mail on Sunday, the British tycoon remained somewhat sanguine about his ambitions for the company.
'We're starting to build a good operation… and I think it can actually be really exciting,' he said.
'I can't say how far we'll go or what we'll do [but] I'm finding it very interesting and absorbing and challenging. There are lots of opportunities, so we'll see how it goes.' he added.
Sorrell left WPP, the company he built up over the course of over three decades after being investigated for misconduct.
In the same interview with the Mail on Sunday the business tycoon refused to answer whether he had used company funds to pay for a prostitute.
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Open Access
Original Article
Factors Associated With Treatment Defaulter among Tuberculosis Patients Registered Under RNTCP in Surat City, Gujarat
Abstract"Introduction: Defaulting from treatment has been one of the major drawbacks to treatment management and represents an important challenge for TB control program. This study was conducted to assess factors associated with treatment defaulter among TB patients in Surat city.
Method: All TB patients registered RNTCP in Surat city from July to August 2016 were include in study. This study was comparing previously treatment defaulter and newly diagnosed TB patients.
Result: In this study observed that male gender, age more than 30 years, migrant status, illiteracy unskilled labour word, lower socio-economic class, tobacco consumption, alcohol habite were significantly associated (P


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Social Welfare Department
Working Family and Student Financial Assistance Agency
Labour Department
Hongkong Post
The Land Registry
Tobacco and Alcohol Control Office, Department of Health
Hong Kong Housing Authority & Housing Department
Greening, Landscape & Tree Management Section, Development Bureau
Agriculture, Fisheries and Conservation Department
Companies Registry
Food and Environmental Hygiene Department
Transport Department
Leisure and Cultural Services Department
Marine Department
Rating and Valuation Department
Electrical and Mechanical Services Department
1823 Mobile App - "Tell me@1823"
phone network. The latest version for iOS is 1.64 and the latest version for Android is 1.63. For other mobile users, you may wish to click here to use browser version of our e-form, which also allows
Font size
will allow you to hold down the Ctrl key (Command key on a Macintosh) and press the + or - key to increase or decrease the text size, and 0 to go back to the default size. On a smartphone or a tablet, you can
About Us
Christmas. 2006 Launch of the website and online forms Provided on the internet the information of 1823 and allowed citizens to submit enquiries, complaints, suggestions and compliments related
nudity, including nudity or partial nudity of children of any age; Content that is unlawful, harmful, threatening, abusive, harassing, tortious, defamatory, vulgar, obscene, libelous, invasive of another


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Apropos Conservatories' Winter Reflections.Published on 31st January 2019
Winter is often a time for introspection. There are two 'Blue Mondays' in January alone! That's reason enough, we think, to consider all the good winter brings, in an attempt to channel some serious PMA. So this week we've been talking about our favourite parts of winter; weather and all!
Here's what Apropos HQ came up with!
Raindrops on [glazed] rooftops – Recently, a Facebook comment alerted us to the fact that not everyone enjoys the sound of rain on a conservatory. For us, it's one of life's pleasures. While we appreciate that trying to sleep in a conservatory in a storm (as the writer of this article has on an unfortunately wet holiday in Pembroke!) is not frustration-free, we're united in finding the pitter-patter of rain on glass soothing and appealing, creating in fact, the ideal ambience for reflection.
Job satisfaction – The thing about creativity is that it's hugely rewarding and there is nothing more sustaining than seeing someone's dreams made concrete… or… erm… glass… so we get a lot of job satisfaction at Apropos. One of our favourite projects this last year has been the Kirmond barn conversion; it was a fantastic, ambitious idea and we love the results so much that we were half-hesitant to hand over the keys when we were done!
Uncoiling Spring – Looking out of the conservatory windows today, with sleet and snow buffeting the glass, we unexpectedly spotted the first signs of spring; crocus and daffodil bulbs poking green noses through the soil. However bad the winter feels, spring always follows.
Cat beards – Sneaky office internet surfing… Need we say more? C'mon, tell us that didn't make you smile!
The future's bright – This year we're excited about all sorts of things; the RHS Tatton Park Flower Show; our Apropos Outdoor Living range; summer holidays; getting back out in the garden; new projects; getting to know all of you lovely people a little better.
Winter is more than halfway over; the days are getting longer; the warm weather closer. It maybe a time for reflection, but try thinking about the good bits – it's happy-making that way.


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Lucky Strike Elbow Patch Tee
Accent those distressed jeans of yours with this three quarter sleeve tee that features bold color blocks and on-trend elbow patches! This is definitely a go-to look for the weekend if you ask us!!!
- Tunic style top
- Made in the USA
- 95% Rayon 5% Spandex
- Sizes: S (4-6) M (8-10) L (14-16) XL (18)


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Injectable foam insulation is a very effective way to prevent large amounts of heat loss, and can be installed into most existing walls, including weatherside, stucco, brick veneer and foundation walls if there is an accessible cavity. The installation can take place from either the outside through the exterior or inside through the drywall of the house, and will usually be completed within one to two days, with very little disruption to the house.
RCI does open and closed cell spray foams for residential and commercial. The two component application process features a wide range of specially formulated polyurethane spray foam offerings that meet specific site and surface requirements. Example of project are. Walls, Basements, Rim Joist, Attics, Garage, Sheds and Buildings Etcetera.
For use in attics, floors and walls
The top problems to list a few in attics are Wet/Moldy Insolation, Rats/Mice, Bats, and other Rodents. These are things that can make a home or office unsafe or even cause problem to peoples health. With are E-Vac system we can get a majority of the problem sucked out of the attic with little to no affect to the rest of the home or office. Once all of the old Insolation / Waste is gone it will leave a new clean area to add Insolation to make it just as great as a new House or Office. Talk to us about are none harmful Bat remove system To!
We also do all kinds of Batt Insolation. This helps with sound deadening and a low cost to your project.


text: Although I am a bit of a blush freak, I refrained from buying orgasm because I refused to believe the hype. Despite the mediocre reviews, I broke down and purchased this product after having several instances of blush over-application issues. I thought a creme version like this might be more sheer. I also noticed that powder blushes were starting to age my skin a bit. Anyway I finally broke down …Read more


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[24 May 2019 | Friday]
ALWAYS Be Prepared!"
"ALWAYS, Be Prepared!"
But in your hearts revere Christ as Lord. Always be prepared to give an answer to everyone who asks you to give the reason for the hope that you have. But do this with gentleness and respect, keeping a clear conscience, so that those who speak maliciously against your good behavior in Christ may be ashamed in their slander. 1 Peter 3:15-16
As a TRUE BELIEVER or a TRUE CHILD of the LIVING GOD, which ever term you prefer, we should ALWAYS, REVERE Jesus Christ as LORD. Which means that we hold Him in DEEP RESPECT, LOVE, AWE and HONOR. When we are in FEAR and DISTRESS, we can say: "The Lord is my STRENGTH, whom shall I FEAR."
And we SHOULD ALWAYS be PREPARED to give an answer to EVERYONE who asks us for the REASON of the HOPE that is in us. When we come to Christ for the SALVATION of our souls, we NEED to SANCTIFY or "set apart" ourselves from this WRETCHED, SIN-LADEN, EVIL, CORRUPT, and DEPRAVED world, and COMMIT ourselves to being OBEDIENT to the LIFE lived in the SPIRIT, and NOT continue to live a LIFE that is lived in the FLESH. A TRUE BELIEVER'S WALK should match their TALK. Their ORAL TESTIMONY should be CONSISTENT with their "set apart" lifestyle.
Their TESTIMONY should be given with GENTLENESS and RESPECT. And NOT given with an ARROGANT attitude. Those who have NO FEAR in the face of PERSECUTION, are able to TESTIFY RESPECTFULLY and with DIGINITY of their HOPE in their FAITH in Jesus Christ. Then they will be able to have a CLEAR CONSCIENCE, and those who speak with MALICE against, the TRUE BELIEVER'S TESTIMONY and GOOD BEHAVIOR in Christ, will be ASHAMED of their SLANDER.
The Apostle Peter is ENCOURAGING his readers, with the mind-set that GOOD BEHAVIOR is the BEST DEFENSE against UNJUST PUNISHMENT and PERSECUTION. Is your HOPE in the LORD JESUS CHRIST? If NOT you are headed for an ETERNITY in HELL, with ALL its HORRORS and NO WAY of ESCAPE. This is NOT a SCARE TACTIC, but it is the TRUTH of the matter. Without HOPE in Christ Jesus you will end up spending your ETERNITY in HELL. Unless, you TURN AWAY FROM your sinful lifestyle, and TURN TO GOD, with a TRUE REPENTANT HEART. And BELIEVING, with ALL your HEART, that Jesus died for YOU, and is ALIVE in Heaven, making intercession for you, before God. Then CONFESS your sins to Him, and ask Him for His FORGIVENESS. Then ACCEPT His gift of SALVATION, and ALWAYS BE PREPARED to SHARE of your HOPE in CHRIST, and of your ETERNITY in Heaven, with ALL its BLESSINGS. Do this NOW, while you still can. He's waiting and coming SOON!!!


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Massachusetts photo exhibit shows prisoners' last meals
'No Seconds: The Last Meal on Death Row'
One decided his dinner would be a testament to gluttony: an enormous tray of Kentucky Fried Chicken, french fries and fried shrimp, topped off with a pile of strawberries.
Another opted for just dessert, eating two pints of ice cream. A third settled for even less: a single black olive.
In "No Seconds: The Last Meal on Death Row," photographer Henry Hargreaves has recreated 10 meals requested by former death row inmates in prisons across America. Hargreaves photographed not just the food they ate but also imagined table settings, from place mats to tablecloths to plates and silverware. The exhibit opened Wednesday at Herter Gallery at the University of Massachusetts Amherst.
Hargreaves, a New Zealand native now living in Brooklyn, N.Y., is a former fashion model who 10 years ago "decided to get on the other side of the lens," as he puts it. He's since built a reputation for photographing odd and quirky subjects — his website (www.henryhargreaves.com) shows an imitation iPad cooked in a deep fryer — that he hopes will "spark a conversation, maybe make you think about things a little differently," he said during a phone call last month from his home.
"No Seconds" was inspired by his discovery last year of a Wikipedia page about final meal requests from condemned prisoners. Reading about what the inmates sat down to eat gave him a new perspective on the topic, he said: "Suddenly it's a real person, it's not just a statistic or a name. This was someone's last meal — what did that request say about the person? I was interested in telling a story with food, and I thought this would be a good way of doing it."
Hargreaves bought the food, prepared the meals, arranged them on dishes, and then photographed them from overhead.
"I wanted (the photos) to show just what someone would have seen as they sat down to eat, wherever it might have been," he said.
Amid an abundance of fried entrees — chicken, bacon and eggs, grilled cheese sandwiches, potatoes — perhaps the strangest image is that of the solitary olive on a ceramic plate, a knife and fork at either side. This was the last meal that Victor Feuger, a Florida man, requested before he was put to death in Iowa in 1963 for kidnapping and murder.
John Wayne Gacy, the notorious mass murderer from Illinois who was executed in 1994, took the opposite tack, chowing down on a greasy meal that included an entire bucket of Kentucky Fried Chicken. Hargreaves presents this meal on a large aluminum tray, the chicken mounded with french fries, fried shrimp and strawberries.
Timothy McVeigh, executed in 2001 for the 1995 bombing of a federal building in Oklahoma City that killed 168 people, settled on two pints of mint chocolate chip ice cream for his last meal. And Ricky Ray Rector, put to death in Arkansas in 1992 for killing a police officer and another man, dined on steak, fried chicken, cherry Kool-Aid and pecan pie — telling prison guards, after he failed to eat his pie, that he was "saving it for later."
Price limit
In preparing his photos, which he first showed online earlier this year, Hargreaves limited his subjects to Americans on death row. Most were limited by state law to a meal not greater than $40 in value, with no alcohol permitted. He said his choices were partly motivated by his curiosity about why capital punishment is still practiced in the United States.
"As a foreigner, the death penalty was not something I was familiar with," Hargreaves said. "It did seem strange." At the same time, he added, "I'm not trying to make a statement about whether it's right or wrong. I do think it's something we should be more aware of. ... I think I'm trying to find a little bit of empathy for these (inmates), something that allows you to see them as human despite what they did."
Trevor Richardson, the curator of the Herter Gallery show, says he was intrigued by Hargreaves' photos when he read about them in a newspaper article earlier this year. He sees a disturbing juxtaposition between what he calls "the sheer banality of the meals themselves" and the harsh reality that consuming the food will be one of the last things an inmate does.
"Presumably, the food items chosen ... hold some appeal to the condemned person from a simple culinary/taste perspective," Richardson wrote in an email. "But might they also evoke memories of family, or childhood, and what are they thinking as they slowly consume and digest the final bite?
"It is a thought-provoking show and — perhaps on a certain level — one of the saddest and most poignant exhibitions I have ever arranged," Richardson said.
Going viral
Hargreaves initially posted nine photos — the Herter exhibit will have 10 — on his blog, where they soon began to attract attention, he said. "At some point I noticed they'd been re-blogged 75,000 times." That attention led to exhibitions of the work, like the upcoming UMass show.
The exhibit is meant to be provocative, Hargreaves said, and the photos have sparked considerable comment. "I've had the whole gamut, everything from 'This is really horrible and you're sick' to just the opposite, people saying they really like what I've done. To me, getting comments is kind of a pat on the back, good or bad."
He's added a new photograph for the last meal of Teresa Lewis, the lone woman in the exhibit. Lewis was executed in Virginia in 2010 — the first woman that state had executed in almost a century — for arranging the murders of her husband and stepson to collect insurance payments. Her last meal included fried chicken, sweet peas, Dr Pepper and German chocolate cake.
As Richardson sees it, Hargreaves' photos inevitably raise questions about the morality and capriciousness of the death penalty.
Lewis' case, for instance, generated widespread opposition, including from novelist John Grisham, because of questions about her mental capacity and because she had not committed the murders herself. Some testimony at her trial indicated that one of the hired killers, Matthew Shallenberger, had masterminded the deaths of Lewis' husband and stepson. Shallenberger and the other killer, Rodney Fuller, were given life sentences rather than the death penalty.
"I think there is something psychologically jarring in the images that inevitably causes one to ruminate on the fact that in this country, significant numbers of people each year are legally killed by the state," Richardson said.
"No Seconds: The Last Meal on Death Row" will remain on view through Oct. 12 at the Herter Gallery at the University of Massachusetts Amherst.


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Using c++. A bank system program. its a small project in which classes used functions used and inheritance used.
in this code a function of create new account verify account and delete and update account used. functions calling is very simple and easy and every detail disused in detail in the form of comments.