text:
Brewed by Blue Owl Brewing
Mixed-Fermentation Sour Beer4.9% ABV36 IBU
Lightly tart, crisp and refreshing. Malt sweetness with a spicy hop profile.
Promote your beer to thirsty patrons looking for it.Learn More
Heads up! Blue Owl Czech Czech Sour Czech Pilsner may not be available near you. Follow this beer to get notified when it's available nearby, try searching in a different area, or discover some similar beer.
Fruit Beer5.0% ABV
Twisted Tea Brewing CompanyCincinnati, OH
Available at very few places nearby
Fruit Beer2.5% ABV11 IBU
Binding-Brauerei AGFrankfurt, Germany
Available at very few places nearby
Schofferhofer grapefruit is a unique combination of sparkling wheat beer and refreshing tangy fruit juice. Mix of 50% Schofferhofer wheat beer and 50% grapefruit juice.
Fruit Beer4.2% ABV13 IBU
Abita Brewing Co.Abita Springs, LA
Available at very few places nearby
A crisp American-style wheat beer sweetened with raspberries for a delicious fruity profile.


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EURUSD Challenging Major Resistance Zone
Want FULL ACCESS To ROSS' DAILY TRADE ROOM? Simply Click The Get Started Button Right Now! Transcript of Video Click Here to receive an email alert when Ross posts a new daily video I'm going to begin the day today on the Euro versus the US Dollar [EURUSD]. I want to start on the Weekly…Details

subset: commoncrawl


text:
Richburg, SC Flavor Delite Inc
YP - The Real Yellow PagesSM - helps you find the right local businesses to meet your specific needs. Search results are sorted by a combination of factors to give you a set of choices in response to your search criteria. These factors are similar to those you might use to determine which business to select from a local Yellow Pages directory, including proximity to where you are searching, expertise in the specific services or products you need, and comprehensive business information to help evaluate a business's suitability for you. "Preferred" listings, or those with featured website buttons, indicate YP advertisers who directly provide information about their businesses to help consumers make more informed buying decisions. YP advertisers receive higher placement in the default ordering of search results and may appear in sponsored listings on the top, side, or bottom of the search results page.
1. J & B Sales Inc
603 Tysons Forest DrRock Hill, SC 29732
2. Nichols Bros Inc
500 Peays Ferry RdWinnsboro, SC 29180
3. Pepsico Inc
8605 Talbert Paige CtCharlotte, NC 28277
4. Atlantic Sweetener Company
3526 Centre CirFort Mill, SC 29715
5. Connelly John and Co LTD John
6329 Mock Orange DrCharlotte, NC 28277
6. Alliant Foodservice Inc
11521 Granite StCharlotte, NC 28273
7. Snyder's-Lance Inc
2283 Iron Works DrClover, SC 29710
8. E X Mark Inc
2526 Plantation Center DrMatthews, NC 28105
9. Pioneer Food Brokers Inc
6919 Heatherford DrCharlotte, NC 28226
10. Bright Harvest Sweet Potato Co
3624 Huckleberry RdCharlotte, NC 28210
11. Rikkos Inc
5712 Stockbridge DrMonroe, NC 28110
12. Harvest Foods Inc - CLOSED
14330 S Lakes DrCharlotte, NC 28273
13. Haddon House Food
578 L And C Distribution ParkRichburg, SC 29729
14. Frito-Lay
400 Lancaster Byp ELancaster, SC 29720
15. Frito-Lay
1034 Quality DrLancaster, SC 29720
16. Pfg Customized Distribution
1441 Fire Tower RdRock Hill, SC 29730
17. North South Wholesale
120 S Main StLancaster, SC 29720
18. Carolina Ingredients
1595 Cedar Line DrRock Hill, SC 29730
From Business: How may we create value for you? As purveyors of the world's finest food ingredients, we take pride in every aspect of our business. From our top quality commerci…
19. Great Food Svc
2377 Dave Lyle BlvdRock Hill, SC 29730
20. US Foods
800 Food Service DrFort Mill, SC 29715
21. Great Food Services
4005 Providence Rd SWaxhaw, NC 28173
22. Snyder's Of Hanover
13515 Ballantyne Corporate PlCharlotte, NC 28277
From Business: Lance manufactures and markets snack foods throughout much of the United States and other parts of North America. The company s products include sandwich crackers…
23. Bradley & Associates
8607 Royster RunWaxhaw, NC 28173
24. Magnolia's Sugar
13 E Liberty StYork, SC 29745
25. Carolina Beverage Solutions
918 Furman CtFort Mill, SC 29708
26. Hormel Food Corporation
11006 Rushmore DrCharlotte, NC 28277
27. US Foods
13034 Ballantyne Corporate PlCharlotte, NC 28277
28. US Foods
105 Prairie Rose CtWaxhaw, NC 28173
29. Snyder's Lance
The thunder salt & vinegar chips are the best chips. They are "Wow".


text:
Caroline Thomas baby details: Emmerdale actor Adam Thomas and his wife expecting second child - here's everything you need to know about the pregnancy
ADAM Thomas is going to be a dad for the second time after conceiving a baby with his wife Caroline Thomas on their wedding night.
[Caroline Thomas/Instagram]
Adam Thomas and is wife Caroline Thomas have announced they are expecting their second baby four months after getting married in August 2017.
Adam and Caroline had been dating for nine years before they tied the knot and share three-year-old son Teddy James Thomas together.
The Emmerdale actor and his dance instructor wife revealed exclusively to OK! magazine they were expecting baby number two.
Here's everything you need to know about Caroline Thomas' pregnancy, from bump to birth as she discusses sex and due date...
[OK! magazine ]
Adam Thomas baby announcement
The couple revealed they were expecting exclusively to OK! magazine, and admitted the pregnancy was a total surprise.
Adam explained: "We always wanted another baby but we weren't planning for it to happen so soon after getting married.
Adam Thomas and Caz Daly's relationship timeline
Adam Thomas and Caz Daly's relationship timeline
"We conceived on our wedding night!"
[Adam Thomas/Instagram]
Caroline Thomas pregnancy sickness
Adam's wife has been suffering with severe morning sickness and ended up in hospital on a drip.
Speaking about the experience, Caroline said: "At one point it got so bad that I ended up in hospital. I was unable to keep any food or liquid down so I had to be put on a drip. I've never known anything like it, I was constantly being sick and I lost nearly a stone in weight."
Adam added: "Caroline's wedding ring fell off because she got so thin. It was so scary, I felt helpless."
Jordan Banjo looks back at his time in I'm a Celeb
Dancing On Ice: Holly asks Jordan to 'take her BUNS'
Shona McGarty shows off stunning vocals on Children in Need
Kate Middleton news today: Latest updates from the pregnant royal
Pregnant Chrissy Teigen and John Legend having a baby boy?
Emmerdale reveals another shocking twist involving Adam Barton
[Caroline Thomas/Instagram]
Adam and Caroline expecting a boy or girl?
The couple have decided they will find out the sex of their baby on Christmas Day.
Caroline explained: "I'm going for a gender scan soon and I'm going to put the result in a sealed envelope, then I'm planning to give it to a lady I know who makes balloons filled with either pink or blue bits hidden inside.
"We'll pop it in front of our families and all find out together."
[Adam Thomas/Instagram]
Caroline Thomas' due date
The couple revealed they were four months pregnant in December 2017, so the due date for their second child is May 2018.


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Cox the Saddler
23 High St, Chesham, Buckinghamshire, HP5 1BG, England. Telephone 01494 775577
|Sign In | Register
|A traditional leather goods store
Order in next 3 hours and 24 minutes for despatch today.
Home | Spares for Leathergoods | Suitcase Keys
Samsonite Suitcase Two Prong Key
We have a Leathergoods Repair Service!. If you need a free quote, just email email@example.com, ideally attaching a picture.
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Product Code: ssk3This is a key for a Samsonite suitcase. Samsonite locks usually have the number on the barrel and it is duplicated on the keys itself so hopefully you will have no trouble identifying the key you require. The drop-down menu shows all the keys we have in stock but if you don't see the number you need give us a call (01494 775577) and we will try to help. These keys are sold individually so if you require a pair please select two in the quantity.
Price: Ł4.20 (Ł3.50 ex VAT)
(Approx. 4.73 USD US Dollars, 3.97 EUR Euros, 534.52 JPY Japan Yen)
To see shipping options, add products to your basket and then View Cart
Would you like to check stock availability? Call Sara or Paul on 01494 775577 (if not from UK please call 0044 1494 775577) Mon-Sat 9.30 a.m. to 5.00p.m. Please Note: We do not accept orders by telephone and enquiries are better placed by email too. When ordering on-line, please note that we do not deduct any money from your credit card, until the goods are being despatched. Please also note that you may alter the quantity of goods in your basket at any time.
Contact us: Telephone 01494 775577 or email firstname.lastname@example.com
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|Sara, I received the Brady Lana bag and I am very satisfied, thank you for your good service.
Kind regards
Minda R., Co. Durham
Dear Paul and Sara, Sue has just let us know that our order of walking stick knobs have arrived already!! Can't believe how prompt you were with our order! Brilliant work and very impressive. Thanks again. Cheers,
Nick M., Australia
Paul hi, I would like to thank for your amazing response to my non-appearing" tiny " order.
The product you have sent without additional payment being made arrived out of the blue today.
I can only say in this day and age customer service is most often not delivered in the way you have.
Thank you, if the other packages ever turn up I will of course return them.
Finally, my sincerest wish is that your business prospers, and for my part I have already and will continue to sing your praises to any one that will listen.
Kind regards,
Chris B., U.K.
Thank you Paul,
Very prompt delivery, fitted new hasps easily, just the job. Much appreciated.
Don. M., Dorset
Paul/Sara, Many thanks for your very quick response and your very nice email.
Wheel arrived this morning, as promised and is now fitted to my luggage. Many thanks,
Jim A., Lanarkshire
Excellent, prompt service yet again. My order arrived within 24 hours of placing it! I genuinely think your service is exceptional.
Happy Christmas to you all, Gill.
Gill, Pinner
Good evening, Brilliant service. Thank you. I wish much bigger Companies my husband and I deal with could aspire to the same high standards.
Good luck with your future business which we will most certainly support.
Hilda W., Newcastle
Hi Sara, The Brady Kent bag has just arrived! Very happy with it and great customer service.
Many thanks.
Jamie S., Warwickshire
I fitted the replacement Gamebird seat yesterday I believe it is better than the original!
Really very pleased with your service and product.
Wishing you great success.
Stuart G., Essex
Dear Paul, I received the jacket today, it only took 7 days to get here. It looks great! Couldn't be more pleased :)
Thank you,
Emma K., California


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Mac Cosmetics Vouchers
. Inside the US by yourself, inside the 12 months 2008, around one,760,000 beauty surgical techniques were being performed (all figures gathered because of the American Society for Aesthetic Cosmetic surgery). 90 % of individuals had been women of all ages, but the proportion of males going through cosmetic operation carries on to boost annually likewise, which can be no less than partly for the reason that corresponding stigmas are getting to be significantly less pronounced and males at the moment are anticipated for being concerned with regards to their appearance too. The top techniques for women with regards to level of popularity are breast augmentation, liposuction, blepharoplasty (beauty eyelid surgery), abdominoplasty (also referred to as a tummy tuck), and breast reduction, whilst the most common strategies for men are liposuction, rhinoplasty (cosmetic surgery with the nose), blepharoplasty, gynecomastia, or male breast reduction surgical treatment, and hair transplantation.
Persons aged 35-50 several years are more than likely to hunt cosmetic operation; this shows that growing old may be the greatest component resulting in people using an desire in beauty medical procedures, probably for the reason that genetics or features existing from start tend to be looked after at a young age. Around 70% of individuals point out that they are not humiliated to mention which they have gained cosmetic surgery, which suggests that cosmetic surgical procedure is actually a socially acknowledged apply.
Read Also: Mac Cosmetics Description


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Last weekend we held our 2nd annual DDA Napa Valley Training camp. I've been living in Sacramento for the last decade but the Napa Valley always feels like home.
What I've learned: Five tips for using an indoor trainer effectively
As the nasty weather begins to set in across the country, more and more of us will be forced indoors on some sort of indoor trainer. Here are some tips to use your trainer time effectively.
Athlete Profile: Cam Juarez
In today's athlete profile we sit down with Data Driven Athlete Cam Juarez to discuss napping, whole wheat pancakes, and zen.
Athlete Profile: Philip Luedtke
In today's athlete profile we sit down with Data Driven Athlete Philip Luedtke to discuss erg mode, mountain bikes, and when to always skip an interval workout.
How to Make Progress on the Bike
Everyone gets better when they start riding a bike, but what happens after you've been at it for a while? Why do some riders continue to improve while others plateau or burn out?


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text:
- More
LOT #95212 |Sold for: Sign-in or Join (free & quick)
The Little Mermaid Ariel Animation Drawing (Walt Disney, 1989)....Click the image to load the highest resolution version.
Claim Item:Share this lot
DescriptionThe Little Mermaid Ariel Animation Drawing (Walt Disney, 1989). This stunning 16 field drawing of Ariel is a beautiful animation rough from the hand of 2013 Disney Legend inductee, Mr. Glen Keane. This was used in the development of the scene for Ariel's performance of her signature song "Part of Your World," from the second verse "Look at this trove, treasures unfold." The dialogue word "Look" is in the upper right border, with an animator's timing chart also on the right side of the drawing. A "127" is written in the upper right corner. A lovely graphite image of 7" x 7.5", this is one of the most striking drawings from this film that we have ever seen. Matted to an overall size of 20" x 16", it is in Fine condition.
Fees, Shipping, and Handling Description: Flat Material, Small (view shipping information)
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Recent Posts
My Account
Month: July 2016
Hillary will get away scot-free
No one should be surprised. The Federalist
If I buy a US flag, I get to burn it if I want to
Cops need to be reminded of that, occasionally. Reason
DEA probably won't legalize medical marijuana
The agency's survival depends on the continued prohibition of the medically beneficial plant. Reason
TSA fails every security test, yet is still proficient in dishing out subhuman treatment
Beating a 19-year old cancer survivor bloody is a top priority for TSA, apparently. Techdirt
Fourth of July resolution, along with oddities of the day
It's been awhile. Far longer than I had intended, anyway. Well, I'm back. I believe that if one is serious about advancing liberty, it must become his or her day job. No more moonlighting, dilettantery, or going AWOL for months at a time. To act like an amateur, a dabbler, is to be an amateur dabbler. […]


text:
(William West / Getty Images)
Australian Open2016 champ Angelique Kerber into 2nd round of Australian Open
German also extends streak to 10 wins
MELBOURNE, Australia — Former champion Angelique Kerber continued her resurgent run with a 6-0, 6-4 win over fellow German Anna-Lena Friedsam to reach the second round of the Australian Open. Full Story
(Jeremy Papasso / Staff Photographer)
Boys basketball: Holy Family finds a way to close against Mead
Baird and Jolly help move Tigers to top of the TVL
BROOMFIELD — It was a game the Holy Family Tigers needed to have. Their recent struggles, especially closing out games, had been a point of emphasis for Tigers coach Pete Villecco and his boys in the recent weeks. Full Story
(Matt York / Associated Press)
College footballCU football: Buffs reeling from first blowout loss
Turnovers, sacks allowed pile up in defeats
After many of Colorado's Pac-12 losses over the years, there was a helpless feeling coming from the Buffaloes.Full Story
(Paul Aiken / Staff Photographer)
Second Story Garage: Gasoline Lollipops
The Boulder alt-country band gives its EPs names such as Death and Resurrection, and its songs bear the mark of hard truths and sin. But the punk energy behind the playing, and the sense that it's all in good fun, make it OK to dance to a song like "Death." Full Story
Local & National Video


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2 University of Chinese Academy of Sciences, Beijing 100049, China
3 Center for Ecological and Environmental Sciences, Key Laboratory for Space Bioscience & Biotechnology, Northwestern Polytechnical University, Xi'an Shaanxi 710072, China
Fireflies, in the family Lampyridae (Coleoptera), are well-known as luminescent insects and include more than 2 000 species in approximately 100 genera of seven subfamilies worldwide (Branham, 2010; Lawrence & Newton, 1995). Different firefly species and their developmental stages exhibit different signaling systems, which play important roles in sexual communication and defense. As such, fireflies are a good model for studying the evolution of luminous signaling systems (Stanger-Hall & Lloyd, 2015; Stanger-Hall et al., 2007), sexual selection, and speciation (Lewis & Cratsley, 2008; Lloyd, 1971, 1973; Ohba, 1983).
Eukaryotic genomes not only contain genetic information but also act as structural components that determine nuclear properties and influence various biological features such as cell size, developmental rate, and developmental complexity (Gregory & Hebert, 1999; Koshikawa et al., 2008). Genome size is described by either mass (pg) or number of base pairs (bp) (Gregory, 2005a). Eukaryotic genome size is important as the basis for comparative research into genome evolution and as an estimator of the cost and difficulty of genome sequencing programs for non-model organisms (Gregory, 2005b; Gregory et al., 2007).
So far, the genome sizes of 5 635 animal species (3 793 vertebrates and 2 429 invertebrates) have been recorded in the Animal Genome Size Database (Accessed 27 March 2017) (Gregory, 2017). Compared to those of mammals (14.14%, 778 of 5 500 species) and birds (8.96%, 896 of 10 000 species), the genome sizes of invertebrates remain poorly studied regarding abundance and diversity. Of the nearly 1 000 000 described insect species, the genome sizes of only 930 (0.093%) have been estimated. Among them, more than two-thirds are from the Holometabolous orders Diptera (254 species), Coleoptera (181 species), Hymenoptera (153 species), and Lepidoptera (65 species) (Gregory, 2017). Coleoptera (beetles) (ca. 360 000 species) is the largest order in the animal kingdom (Bouchard et al., 2011, 2009), and its 181 species with reported genome size estimates are mainly distributed in nine families (Tenebrionidae: 69; Chrysomelidae: 65; Coccinellidae: 39; Dermestidae: 6; Scarabeidae: 3; Dytiscidae: 2; Carabidae: 1; Geotrupidae: 1; Silvanidae: 1). For the luminous beetle family (Lampyridae), the genome sizes of 23 species from North America have been described recently (Lower et al., 2017). Here, we report on genome size estimations of 14 firefly species from China.
To explore firefly genome size evolution and estimation of the cost and difficulty of future genome sequencing programs, we performed C-value measurements for 14 firefly species (two genera in Lampyrinae, three genera in Luciolinae, and one genera in subfamily incertae sedis) using flow cytometry. Although many methods for the estimation of genome size have been described, most genome size estimates in both animal and plant species estimations have been conducted using flow cytometry (Galbraith et al., 1983; Gregory et al., 2013; Hare & Johnston, 2011). We also constructed a phylogenetic tree of the 14 species using a mitochondrial cytochrome oxidase subunit 1 (COI) gene fragment and discussed firefly genome size evolution in the phylogenetic context. The relationships of genome size to morphological traits such as body length, body width, antennal length, and eye width were also described.MATERIALS AND METHODS Sampling and observation of morphological characteristics
Specimens of 14 firefly species from Yunnan, Hainan, and Hubei provinces of China were used for genome size estimation and body size measurement (Table 1). Some live specimens were used for estimation of genome size, with the remaining samples kept in 75% alcohol for morphological observation and body size measurement. All morphological observations and measurements were carried out under a dissecting microscope (SMZ 800, Nikon, Japan) according to Jeng et al.(2007). All measurements were based on male adults as females were difficult to collect. The abbreviations BL, BW, EL, ELW, PL, AL, and EYW represent body length, body width, elytral length, elytral width, pronotal length, antennal length, and eye width, respectively. BL is the sum of PL and EL (BL=PL+EL), BW is the greatest distance across the elytra, and EYW denotes the smallest interocular width (measured horizontally). Male genitalia were also dissected and examined under a dissecting microscope to help with specimen identification. According to previous morphological descriptions (Ballantyne et al., 2013; Jeng et al., 2000), all species were at least assigned to genus. For the four species with both male and female samples, live specimens collected at the same locality and time were observed to mate. Combined with their morphology, we confirmed they were of the same species.
For the males of each species, the brains of 3-6 live specimens were dissected for estimating genome size, with the thoraxes and abdomens were directly kept in -80 ℃ for genomic DNA extraction of single individuals when necessary. At least four males for each species were kept in 75% ethanol as voucher specimens. For females of the four species (Lamprigera yunnana, Abscondita terminalis, Pygoluciola qingyu, and Pygoluciola sp1), brains of 4-6 live specimens were dissected to use for estimating genome size.Flow cytometry
Genome size was estimated using flow cytometry (Bennett et al., 2003; Li et al., 2015). As with genome size estimation of other insects, such as the ladybird beetle (Gregory et al., 2003) and butterfly (Jiggins et al., 2005; Li et al., 2015), the model insect Drosophila melanogaster (genome size 176 Mb) (Bosco et al., 2007; Gregory & Johnston, 2008) was selected as the standard. Brain tissue from single firefly adults or larvae and the heads of 10 Drosophila melanogaster (Dm) adults were dissected under a dissecting microscope (SMZ 800, Nikon, Japan) and added to 60 μL of cold Galbraith buffer (Galbraith et al., 1983) in 1.5 mL Eppendorf tubes in Pestles (Sigma, USA) issue grinder, stroked 40 times with a pestle, and then added to cold Galbraith buffer to get a final volume of 400 μL for Lampyridae and 1 000 μL for Dm. Except for Pyrocoelia pygidialis, we prepared cell suspensions from 3-6 males and 4-6 females of Lampyridae as biological replicates. For P. pygidialis, only two larva individuals were used as biological replicates because no live adults were collected during the experimental period. Finally, the Dm and firefly cell suspensions were filtered through a 20 μm nylon filter. After this, 50 μL of the Dm cell suspension was added to 1.5 mL Eppendorf tubes containing 350 μL of the Lampyridae cell suspension. Propidium iodide was added to a final concentration of 50 parts per million, and the mixture was co-stained in the dark at 4 ℃ for 30-40 min. The fluorescence of co-stained nuclei for each sample was quantified using an LSR Fortessa (BD, USA) with the laser tuned at 561 nanometers. The DNA content (pg) was determined by comparing the ratio of the 2C mean of the tested samples with the 2C mean for Dm (1C=0.18 pg) (Bennett et al., 2003; Galbraith et al., 1983). Genome size (bp) was calculated from DNA content (pg) following the formula (Dolezel et al., 2003): genome size (bp)=(0.978×109)×DNA content (pg). According to this formula, each C-value was calculated based on the main peak of the 2C cells.DNA extraction, PCR amplification, and sequencing
The genomic DNA of fireflies was obtained from the thorax and abdomen of a single male individual. DNA extractions were performed using a Gentra Puregene Blood Kit (Qiagen, Germany) following the manufacturer's protocols. The primers C1-J-2183 (5'-CAACATTTATTTTGATTTTTTGG-3') and TL2-J-3014 (5'-TCCAATGCACTAATCTGCCATATTA-3') (Lower et al., 2017; Simon et al., 1994) were used for amplification of the a part (about 800 bp) of the mitochondrial COI gene. The 20 μL reaction mixture consisted of 10 μL of 2×Trans Direct PCR SuperMix (Trans Direct Animal Tissue PCR Kit), 1 μL of forward primer (C1-J-2183) (10 μmol/L), 1 μL of reverse primer (TL2-J-3014) (10 μmol/L), 1 μL of DNA template, and 7 μL of ddH2O. The amplification protocol was as follows: initial denaturation and enzyme activation for 5 min at 94 ℃, followed by 35 cycles for 30 s at 95 ℃, 30 s at 55 ℃, 60 s at 72 ℃, with a final extension of 7 min at 72 ℃, and 10 ℃ hold. The PCR products were electrophoresed using 1% agarose gel and sequenced by BioSune BiotechnologyCo., Ltd (ShangHai, China).. The COI sequences of seven species were from our firefly mitogenome project (MG200080-MG200086); and those of the other seven species were from the current study and were deposited in GenBank under accession numbers (MF375910-MF375916).Phylogenetic analysis
All sequences were aligned using ClustalW and analyzed using MEGA 7.0 software (Kumar et al., 2016) and MrBayes version 3.1.2 (Huelsenbeck & Ronquist, 2001). Interspecific and intraspecific sequence divergences were calculated using the General Time Reversible (GTR+G+I) model with the pairwise deletion option in MEGA 7.0. Based on the GTR+G+I model, maximum likelihood (ML) tree was constructed using MEGA 7.0. Node supports for ML were inferred with bootstrap analysis (500 replicates). The Bayesian tree was established with MrBayes Version 3.1.2. The GTR+I+G model was selected via Modeltest version 3.7 and MCMC was run for 300 000 generations. The average standard deviation of split frequencies reached a value less than 0.01, with the Bayesian posterior probabilities calculated from the sample points after the MCMC algorithm started to converge (Zhan & Fu, 2011). Rhagophthalmus lufengensis and Rhagophthalmus ohbai (GenBank accession No. DQ888607.1 and AB267275.1, respectively) were used as outgroups (Li et al., 2007). We used molecular phylogeny to correct for nonindependence of related species (Felsenstein, 1985; Lower et al., 2017).Analysis of relationship between body size and genome size
Body size measurements, including BL, BW, AL, and EYW were determined based on 4-5 male individuals (Table 2). The relationships between genome size and body size were plotted using ggplot2 (Wickham, 2016). Phylogenetic generalized least squares (PGLS) in the R package nlme (Pinheiro et al., 2017) was used to analyze correlations between genome size and explanatory variables.
Considering that identification of fireflies at the species level is still unclear, especially for those species distributed in China, we assigned some specimens as species incertae sedis (sp) at a defined genus, and described their morphology (Figure 1, Table 2). Lamprigera was placed in the subfamily incertae sedis (Martin et al., 2017). Three species of Lamprigera had similar outer shapes (Figure 1A-C), but could be separated by their genital morphology. Three species of Diaphanes were easily separated by their antennae (Figure 1D-F). Four species of Pyrocoelia were separated by their wing and luminous organs (Figure 1G-J). Four species of Luciolinae were separated into three genera, including Abscondita, Pygoluciola, and Luciola by their wing, abdomen, luminous organs, and genitalia (Figure 1K-N).Firefly genome size and evolution
Flow cytometry showed distinct peak(s) for the different species (Figure 2). Nuclei from the heads of the 10 Dm specimens and the brain of a single Lamprigera sp3 male produced a single, broad 2C peak (Figure 2A-B), whereas mixtures of the heads of D. melanogaster and brain of the Lamprigerasp1 male produced two broad 2C peaks (Figure 2C).
The haploid genome sizes of Lampyridae males ranged from 0.42 (Pyrocoelia sp3) to 1.31 pg (Lamprigera sp1) (411 Mb to 1 281 Mb) (Table 2), demonstrating 3.1-fold variation (Table 3). For four species: Lamprigera yunnana, Abscondita terminalis, Pygoluciola qingyu, Pygoluciola sp1, we also estimated the genome sizes of female individuals, which were found to be similar to those of the males (Table 4).
To explore the evolution of genome size within Lampyridae, we constructed a molecular phylogenetic tree for the tested species using the mitochondrial COI sequences, which supported morphological taxonomy at the subfamily and genera levels (Table 1, Figure 3).Relationship between genome size and body size in fireflies
We explored the relationships between genome size and body size measurements, including BL, BW, AL, and EYW (Table 2). Our data showed no significant associations between firefly genome size and BL (r2=0.011, P=0.726, λ=1), BW (r2=0.016, P=0.669, λ=1), EYW (r2=0.11, P=0.241, λ=1), and AL (r2=0.045, P=0.469, λ=0.996) (Figure 4). We further performed PGLS analysis between BL, AL, EYW and phylogeny. The parameters of AL, EYW (λ=1), and BW (λ=0.996) indicated complete dependence on genome size between phylogeny and morphological traits. Pagel's parameter estimates for genome size supported a Brownian motion model of evolution and complete phylogenetic dependence (λ=1.00, 95%) supported a neutral model (Lower et al., 2017).DISCUSSION
Based on 39 species in 27 genera, the family Coccinellidae shows a large 26-fold genome variation (0.19-5.02 pg) (Table 3), with a considerable 21.7-fold variation also detected in Chrysomelidae (0.17-3.69 pg) according to 65 species in 27 genera (Gregory, 2017). A small 1.2-fold variation of genome size is reported in the family Dytiscidae (1.01-1.22 pg), though this is based on estimates of only two species. Our data from 14 species of six genera showed that the male haploid genome size in Lampyridae exhibited 3.1-fold variation (Table 3), which is relatively small compared to those of other currently estimated beetle families (Gregory, 2017) (Table 3). Nevertheless, compared to 2 000 species in more than 100 genera of seven subfamilies, the tested species in this study accounted for only a small proportion. Thus, more species, subfamilies, and genera, as well as different geographical distributions, are needed to better explore the evolution of firefly genomes. As Gregory (2002) states, the C-value enigma is a 'complex and multifaceted puzzle, immune to one dimensional explanations'.
Based on the phylogenetic relationship of the 14 species, our data suggest that genome sizes are very varied in Lampyridae. The Lamprigera species in subfamily incertae sedis exhibited a relatively large genome size of more than 1 pg (Table 2; Figure 3), which is less than 2-fold that of some Pyrocoelia species. The genome sizes of both Lampyrinae and Luciolinae ranged more than 2-fold. In Lampyrinae, Pyrocoelia species had relatively small genomes, spanning 0.42-0.75 pg (411-737 Mb), including the smallest known genome (0.42 pg, 411 Mb) in Lampyridae (Table 2); Diaphanes species showed relatively large genome size variation, spanning from 0.53-1.2 pg (513-1 174 Mb), in which Diaphanes sp2 and Diaphanes nubilus, despite being closely related (Figure 3), showed 1.17-fold genome variation (Table 2). In Luciolinae, the genome sizes of Pygoluciola sp1 and Pygoluciola qingyu were 0.74 pg (728 Mb) and 1.21 pg (1 096 Mb), respectively; Abscondita terminalis had a relatively small genome (0.5 pg, 491 Mb), but related Luciola (L. sp6) species had a large genome (1.29 pg, 1 259 Mb) (Table 2; Figure 3).
Except for Lamprigera yunnana, three species in Luciolinae exhibited slightly larger genomes in females than in males. According to karyotypic analysis of species in the subfamilies Lampyrinae, Luciolinae, and Photurinae, Lampyridae frequently showed X0/XX karyotype sex determination, with males of X0 and females of XX (Dias et al., 2007), possibly explaining the slightly larger genome size in females than in males. Combined with the facts that the neoXY type was also reported from one species in Photurinae (Bicellonycha lividipennis) and the supernumerary chromosome found in some species of Lampyrinae (Dias et al., 2007) and that Lamprigera still has a disputable position at the subfamily level (Jeng et al., 2000; Li et al., 2006), it is too early to explain the slight differences in genome size detected between males and females of this genera. Further karyotypic analyses of these genera should help to settle this question.
Our data showed no significant association between the firefly genome size and morphological traits such as BL, BW, and EYW (Figure 4). Previous data also support no correlation between genome size and body size in the beetle family Coccinellidae (Gregory et al., 2003) and in North American species (Lower et al., 2017). However, for the Pimelia and Phylan genera in the beetle family Tenebrionidae, negative correlations between genome size and body size have been reported (Palmer & Petitpierre, 1996; Palmer et al., 2003). For other insects such as aphids (Finston et al., 1995; Gokhman et al., 2017) and mosquitos (Ferrari & Rai, 1989) and other invertebrates such as turbellarian flatworms (Finston et al., 1995) and copepods (Gregory et al., 2000), a positive relationship between body size and genome size has been described.
Although the study of animal genome size has been ongoing for more than half a century, there is still a need to estimate the genome sizes of more animal groups by flow cytometry and further explore the evolution of genome size. Fast though costly next-generation sequencing technology will provide a complementary role for genome surveys, including genome size and complexity (Li et al., 2015). In summary, our study provides an estimation of the cost and difficulty of genome sequencing programs for non-model organisms, and will help promote studies on firefly genome evolution.ACKNOWLEDGEMENTS
We would like to thank the anonymous colleagues and villagers for help in collecting the firefly specimens used in this study. We also thank Lei Chen and Wei Liu for their comments on this manuscript
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Whether you're a long-term resident of Calgary, or if you're new to the area, this website is designed to help you easily search for Calgary real estate. Explore the cities various neighbourhoods and view our featured listings.
Want to know what your home's value is? In today's market, it's more important than ever to price your home right. Use our home evaluation tool and we will compare your home to active Calgary real estate listings.
Purchasing a house is never easy. That's why you need an accredited REALTOR® with local knowledge and the experience to guide you. Team Ken Richter has offered premium service and quality client care for over 30 years.
When it comes to listing your Calgary home for sale and investing in Calgary real estate, you want to know you're working with the best. Ken Richter has been one of the finest Calgary real estate agents for 30+ years. Born and raised within the city, Ken has an intimate knowledge of the local area. Whether you're new to the area or you've lived in the city for decades, Ken can help you find a home in a neighbourhood that suits your lifestyle. If you're looking to be close to the vibrancy of the inner city, your child's school, or within walking distance to your work, Team Ken Richter has the local knowedge and the experience to make your real estate dreams a reality.
For a Calgary homebuying or homeselling experience that's simple, contact Team Ken Richter. They have a track record of delivering top results. Learn more about what past clients have to say on our Testimonials page or take a look at our blog for regular updates on the Calgary real estate market and home renovation ideas.
Ken Richter has been my realtor for many years, with buying and selling... referrals for other properties... I can honestly say that Ken has always had my best interest at heart. He makes sure you stage your home for a seller and I can say, it works. His expertise is bar none, spot on with efficient staff to help. He is one of the best and his record shows it. Go Team Richter!
Ken got the job done quickly and efficiently. No hassles!
I was very happy working with Team Ken Richter. They were attentive and very supportive. There were a couple extra matters needing attending to and they went the extra mile for us on both of them. Thanks Team Ken Richter!
The trademarks REALTOR®, REALTORS® and the REALTOR® logo are controlled by The Canadian Real Estate Association (CREA) and identify real estate professionals who are members of CREA. Used under license. The trademarks MLS®, Multiple Listing Service® and the associated logos are owned by The Canadian Real Estate Association (CREA) and identify the quality of services provided by real estate professionals who are members of CREA. Used under license.


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Ayala to Boost Profit by More Than Half as Spending Hits RecordClarissa Batino and Norman P. Aquino
Ayala Corp. is seeking to boost profit by more than half in two years after allotting record spending this year to increase sales at its property, banking, telecommunication and water units.
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The best way to stay up to date on current events and information regarding Oaks of Weymouth is to subscribe to the newsletter. We publish every other month, and subscribers get an email blast when the latest one is ready.
Learn about community happenings, see upcoming events, and read articles of interest on a wide range of topics. If you're new to our publication, click here to subscribe now. If you are missing email notifications from us, please check your spam folder.
It's the most wonderful time of the year! The best gift of all is to have you as our residents. We hope that you are able to enjoy this holiday season with friends, family, and those you love the most. Thank you for being valued neighbors in our community.
Review our list of upcoming events at Oaks of Weymouth. There is something for everyone to enjoy!
The holidays mean good food and even better company. Check out these savory meals that are fun to cook and even better to enjoy. Whip up something tasty in the kitchen and spend holiday time with loved ones.
The management office will be closed December 25, in observance of Christmas and January 1, in observance of the New Year.
If you're ready to play an assortment of card and board games, then join us every second Wednesday of the month for Game Night. Don't know how to play? We'll teach you! If you know a fun game and want to teach us how to play, we're also happy to learn. The next two game nights will be on December 13 and January 10 at 7 p.m. in the Clubhouse.
We have four Movie Matinee Mondays coming up over the next couple months. Mark your calendars now so you can join us December 18 and January 8 and 22 in the Clubhouse at 1 p.m. Remember, snacks will be served. If you have a movie suggestion, submit ideas to the office.
Back pain is the second most common reason for doctor office visits. If you suffer from this, you know it can be difficult to enjoy everyday activities. Check out these gentle stretches to ease the discomfort so you can continue living pain free
Thanks to everyone who participated in our November Potluck. We had a wonderful time, which you can see for yourself in the photo slideshow below. Congratulations to our winners: Marie Russo, Florence Fagan, and Peggy Milano!
Our Third Annual Halloween Pet Parade was a howling success. We have the winners for all five categories. Thanks to all who participated! Warning: clicking below leads to adorable pet pictures. Prepare for cuteness overload.
Stay current with the latest trash schedule to help keep Oaks of Weymouth in pristine condition.
Make your front porch pop with fall containers filled with beautiful blooms and foliage. Southern Living provides the best ideas and tips for how to bring a beautiful fall planter to life. Whichever you choose, your home will be decorated perfectly for the season.
The Oaks of Weymouth will be participating with Toys for Tots this holiday season. You can drop off new, unwrapped toys now through December 10 at the Clubhouse. The box is located in the lobby area outside the Management Office.
If you have the means to donate to others this holiday season, we ask you to help with the Atlantic County Utilities Authority annual food drive, supporting the Southern Branch Community Food Bank of New Jersey. Donations will be accepted from December 4-15. Click to learn more.
It is important for resident safety that your front lamppost is in working order! If your light does not work, please replace the light bulb and check the circuit breaker. If that doesn't work, the problem is most likely the sensor. Contact the Office at (609) 625-6257 if this is the case and we will replace the sensor for you.
Don't let the hustle and bustle of the season stress you out. Make your travel plans effortless using these holiday travel tips. Being mindful and strategic while you travel will ease the stress and make the season more fun!
Residents can take advantage of our complimentary holiday recycling services after taking their tree down. Holiday trees will be picked up curbside on Thursday, January 11 only. Trees may not be left out at the curb until the day before pick-up.
Calling all knitters! Join our efforts to provide scarves and other goods for the homeless, veterans, and underprivileged infants. Click to learn more.
There are several local food pantries open to the Oaks of Weymouth community. You can use these pantries or make donations to help keep them running.
There's no greater pleasure than delving into a good book and not putting it down until you've read every page. The adventure you take while reading can truly make the world fade away. Click here to see the most popular books of 2017 so far, and add some to your reading list.
Take advantage of one of our many class offerings here at Oaks of Weymouth. Click below to view the upcoming schedule.
Help support our troops by donating goods and other items in the spirit of American patriotism. We have placed a donation box for our troops inside the Clubhouse entrance. Click to learn more.
Ready for some mental stimulation? Join us for "Feed Your Brain," featuring exciting crafts and hobbies instructed by community residents. The group will meet every other Thursday from 2 – 4 p.m. in the Community Center.
It goes without saying that a good night's sleep is a valued part of life. With every day hustle and bustle, many factors often interfere with getting enough rest. Read more for tips on the lifestyle choices you can make to get better sleep.
If you've had a recent change in phone numbers or e-mail addresses, please take a moment and let us know. Stop by the office with the new information or call us at (609) 625-6257.
Need some extra cash? Refer your friends to Oaks of Weymouth and get a reward of up to $1,000 when they buy a new home!
In case of emergency, be sure to keep these numbers handy!
New Jersey State Police – Buena Barracks
Dispatch (609) 625-1104
Station (609) 561-7267
The holidays are a great time to spend with friends and family. Don't let the potential stress of the season get in the way of everything you should enjoy. If you're looking to do less instead of more this Christmas, read more for some tips.
As technology changes and improves, so do the tactics and strategies of scammers and thieves. While this can be frightening, there are ways to prevent and stop it. Here are a few tips from retired police officer, Donna Brown, on how to keep yourself safe online.
Hometown America is proud to promote SafeHaven National Insurance Agency, specializing in coverage for manufactured homeowners and renters. Many companies are difficult to work with or do not write policies that provide adequate protection for prefabricated homes. Make sure you are dealing with an insurance company that offers competitively priced policies and the protections important to you. Visit SaveHaven online or call 1-800-536-8999 for a quote.
Take the hassle out of making rent payments with Hometown America's E-Z Pay. With E-Z Pay, your lot rent will be automatically deducted from your bank account on the third of each month. New sign-ups earn a $10 credit.


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Sort by Price Bed & Breakfast accommodation in Dublin from ( GBP£0.00 | UDS$0.00 ) per room
B&B Directory As a public service, irish-bnb.com carries a directory of all accommodation in Dublin
Sort by Price Bed & Breakfast accommodation in Dublin from ( GBP£0.00 | UDS$0.00 ) per room
B&B Directory As a public service, irish-bnb.com carries a directory of all accommodation in Dublin
|Display rates in:
|Claremont House B&B Dun Laoghaire, Dublin
Rooms From
|Superb Victorian home , built in 1876 with modern updates , free Wifi, free parking, close to the DART (2 minutes walk) buses and ferryport and bus to the airport and is in a quiet cul-de-sac....
|Arus Mhuire B&B Templeogue, Dublin
Rooms From
|Situated in Dublin's quiet South side of the city, Arus Mhuire is just ten minutes from the famous Dublin mountains and a variety of amazing places to visit. This seven bedroom guest house is also just twenty minutes from the city centre by bus. It is the ideal location for any visitor wanting to check out what Dublin has to off...
|Evergreen B&B Malahide, Dublin
Rooms From
|Enjoy a warm and friendly welcome in luxurious spacious accommodation set on one acre of landscaped garden. All rooms with free WiFi, satellite T.V. , hairdryers and ensuite bathrooms. Private free parking, breakfast menu. Situated 10 minutes from Dublin Airport and 20 minutes from Dublin ferry and city centre and one mile from ...
|Parkway Guesthouse Dublin City, Dublin
Rooms From
|Parkway Guesthouse in Dublin, Ireland, is a family-run accommodation that operates in a late 18th century Georgian-style house. Located close to the Temple Bar area, Trinity College, and several music and shopping venues, this cosy bed & breakfast in Dublin is an ideal place to stay for those traveling on a budget. During...
|Seamount House B&B Swords, Dublin
Rooms From
|Welcome to Seamount House Bed & Breakfast, home of the Reilly Family. Our B&B is Situated just 4 km from Dublin International Airport and a stones throw from Swords Village, Castle and the Pavilions Shopping Centre. Also a short walk from the Scenic village of Malahide and the beatuiful Swords Estuary. Here at Seamou...
|Avoca House B&B Drumcondra, Dublin
Rooms From
|Avoca House Bed & Breakfast situated one mile from Dublin City Centre offers luxury bed and breakfast accommodation for tourists and holidaymakers. For a long vacation or just a mini break, Avoca House caters for the guest's every need. Situated close to the airport, botanic gardens, Irish pubs and bus stops etc; Avoca hou...
|Gleann Na Smol B&B Howth, Dublin
Rooms From
|Gleann-na-Smol is located in the centre of the village of Howth close to Howth Harbour and cliff walks. We offer a friendly warm and comfortable home with plenty of Irish charm. Homemade soda bread, brown bread and home made preserves are a speciality in our home. We are members of Bord Failte and Fingal Tourism. We are highly r...
|Harveys Guesthouse Dublin City, Dublin
Rooms From
|Welcome to Harveys, a family run Guesthouse 800m from O'Connell Bridge, in the exact centre of Dublin City, Ireland. We take great pride in providing a comfortable, clean and well run guesthouse for your stay here in Dublin. Our rooms have been completely renovated, with modern fittings, French sleigh beds, TV's, and telephones....
|Saint Marthas B&B Drumcondra, Dublin
Rooms From
|Welcome to Saint Martha's B&B, a friendly, family-run Bed and Breakfast in the centrally located Drumcondra area of Dublin. Here, guests can expect a warm and friendly welcome in a relaxed and comfortable house. All rooms have TV, hair-dryer, toiletries and tea/coffee facilities. And there is a patio area to take advantage ...
|Applewood B&B Drumcondra, Dublin
Rooms From
|This is a lovely 1920's home, fully restored with wooden floors, private ensuites & old oak furniture. We are ideally located 1 mile from Dublin City center and 4 miles from the Airport. A great place to use as your Dublin base for a few days at the start or end of your vacation. Enjoy numerous Restaurants, Pubs, Shops, Taxi...
|Do you own a B&B, guesthouse or hotel in Dublin? Click here to find out how you can become a member of the B&B Network.
NOTE: All displayed rates are double/twin room per night. Exchange rates are based on current bank rates as per ECB.


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Off the Market
Single Family
This listing is courtesy of Barbara Mylonas from Coldwell Banker Rb/Center Harbor
Wonderful 4BR/ 3 bath country home with a 2 BR/ 1 bath guest apt. above the 3 car garage. Complete privacy, yet a short drive to Rt. 25...an east west NH Hiway. Quiet & peaceful. 10 acres with a 2 stall horse barn, fenced pasture and fenced dog yard. Mountain and pasture views. The entire home has been renovated since the current owners bought it. The house and garage have new metal roofs, are currently being painted (almost done) and the inside is entirely redone also. Updated kitchen with SS appliances, new sunroom overlooking the views, new ceiling fans in all rooms, interior newly painted, new pine floor upstairs with a newly remodeled , large and beautiful master bedroom with gorgeous bath and W/I closet. This fine home has been a farm and a B&B in past lives, now it is your turn to use your imagination. This home would be perfect for a large family who loves the country or a family who would needs an inlaw apt, which would also make a great rental to help with the house payments. 2 wood stoves and a whole house generator to help with bad weather. The older part of the home is post and beam and exudes charm and character. Mature plantings abound. Circular driveway. New deck and a large screened porch. Many areas to entertain in. Separate office with door for privacy. Surrounding land in conservation trust & surrounded by riding trails. Tamworth is a fun town with a distillery, museum,summer theatre & farmers market.
Interior Details
2,592 sq. ft.
$129.24/sq. ft.
Dishwasher; Dryer; Exhaust Hood; Microwave; Range - Gas; Refrigerator; Washer
Leach Field; Private; Septic
Drilled Well
Ceiling Fan; Dining Area; Hearth; Master Br W/ Ba; Natural Woodwork; Walk-In Closet; Laundry - 1st Floor
Exterior Details
10.00 acres
435,600 sq. ft.
Detached; Auto Open; Finished; Heated; Storage Above
3 Garage Spaces
Driveway; Garage; Parking Spaces 6+
Post And Beam; Wood Frame
Other Details
Room Levels, Dimensions and Features
There were 1,074 households out of which 29.1% had children under the age of 18 living with them, 50.7% were married couples living together, 7.6% had a female householder with no husband present,...Read More


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