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Tuensang District Adhoc Teachers Association (TDATA) reiterating its earlier ultimatum submitted to the department stated the association was not against the department, or to create enmity but to reach the goal through peaceful means. Tuensang District Adhoc Teachers while lauding the education department for their prompt response and as per assurance given by the additional director and legal officer in charge of education department on May 30, also believed the education department and P&AR department were putting in efforts to fulfill its demand.TDATA with the assurance given by the department, conducted a meeting on June 4 and decided to call off the second phase agitation. In this regard, the association has asked the authorities to speed up the process involved in fulfilling its demand and also requested the P&AR department and cabinet ministers to take the matter seriously. The association further said if the department fails to fulfill its demand, then it would have no alternative but to take its own course of action.
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The ability to convert the leads you get is going to be the ultimate barometer of success for your startup. Procuring leads is a function your marketing team can help you with. Now you might assume that marketing a startup and relatively new brand would require a lot of money. It's true that having the funds and resources to invest in a ... Read More »
Tag Archives: how
How To Grow Your Business Through Advocacy Marketing
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When:Wednesday, 28 Sep 2005
Who:Brandon Neuenschwander
Details:Brandon shoveled down four (4) pounds of flavorful vanilla yogurt in nine (9) minutes. Within the first three (3) minutes, he had obliterated the first of the two (2) 2-pound vats of the bone-and-teeth-friendly treat. About 1/2 way through the second vat, Brandon stopped to enjoy some fresh Pepsi. After the ensuing belch-fest, he got back to it, finishing the second vat at about the nine-minute mark. Done! Lookers-on observed the ghostly whiteness that had come over Brandon's complexion, some fearing that failure (regurgitation) was imminent. Like a rock, Brandon did not falter; he kept the yogurt down. His skeletal system rejoiced, "I'm gonna be so much stronger, now!"
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When:Friday, 30 Jan 2004
|See it! (640 x 618px)
Who:Brandon Neuenschwander
Details:Brandon snarfed down two (2) heads of lettuce from Golden Corral in just about nine (9) minutes. The lettuce was accompanied by one-half of a bowl of french dressing. He really attacked that roughage, enjoying every delicious bite. P.S., this was Brandon's first successful attempt.
Most egg rolls eaten (Previous record)
When:Thursday, 04 Dec 2003
Who:Brandon Neuenschwander
Details:Brandon ate ten (10) egg rolls from King Chef in 55 minutes. He doused the rolls with mustard- and/or duck sauce.
Did not achieve goal! Brandon was attempting to eat a dozen (12) King Chef egg rolls. He blamed the rolls' texture for his failure. He also claimed that the amount of deep fried cabbage that he consumed would make him into a salad shooter the next day.
When:Thursday, 21 Aug 2003
Who:Brandon Neuenschwander
Details:Ate five (5) full cheeseburger Happy Meals (w/ water as drink), and the cheeseburger and drink from a sixth Meal.
Did not achieve goal! Brandon was attempting to eat six (6) full cheeseburger Happy Meals
When:Friday, 20 Jun 2003
Who:Brandon Neuenschwander
Details:Ate 10 Hardee's Slammers, 1 large order of fries, and 1 large Dr. Pepper
-[Show all record entries for Most Slammers from Hardee's ]-
Most White Castle food in one sitting (Tie) (Previous record)
When:Friday, 06 Jun 2003
Who:Brandon Neuenschwander
Details:Ate 11 White Castle hamburgers
-[Show all record entries for Most White Castle food in one sitting ]-


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Join us at our regular church services
Weekly services are from 'Common Worship' (2000) unless stated otherwise.
- 8am: Holy Communion (BCP)
- 10am: Priory Eucharist sung by the Priory choir with Sunday School ('All Age' on the first Sunday of the month)
- 11.30am: Choral Mattins (BCP) on 1st Sunday of the month
- 6.30pm: Choral Evensong and Sermon (BCP)
- Tuesday: 10.15am: Eucharist (BCP)
- Wednesday: 12.30 Eucharist with Prayers for Healing
Thursdays at Penny's Hospital Chapel
- 12.30 pm: Lunchtime Service commencing 1st March
- [St John's Thursday Lunchtime Service is postponed until further notice]
For more information about this week's services and events at The Priory please click here.
Click here to view our Service and Music lists.
Latest News
January Newslink
Our regular magazine 'Newslink' is now available to view online....Read More...
For the Fallen
For the Fallen by One Minute Wonder Films, featuring The...Read More...
November Newslink
Our regular magazine 'Newslink' is now available to view online. Please click below for the latest issue.Read More...


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Nancy Harris, clinical manager at Restorative Care, has gathered old nursing paraphernalia throughout the years and put it on display for colleagues to enjoy during Nurses Week at Baptist Memorial Hospital-Memphis. The display included textbooks and hand-written notebooks from the 1920s, a physician's bag filled with supplies and nursing caps from coworkers when they graduated.
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BMG physician gives invocation for Elvis memorial service
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C program to reverse the contents of a file
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But now that I implemented the print_reversed_string function and wrote the code to reverse the contents of the file in there it still compiles but when I run it it just shows: "Original Text: To be or not to be". } printf ( "\ nContents int main(). . program to reverse the contents of a file; reversing contents of a file using file pointer; fs program for reversing thre file content Greetings, Anyone have a bit of C code that will read a text file and output the There are a number of things in the program I'd probably do Oct 8, 2010 I have a following problem with my C program: user can choose the following menu options to manipulate a file. while (c != EOF). FILE *fptr;. h> #include<fstream>Write a function reverse that reverses the character string s; use it to write a program that reverses its input a line at a time */ #include<stdio. If my Unix system supported the -r Jun 3, 2013 is a directive to the C preprocessor. tac command displays contents of the file in reverse order Feb 4, 2017 Just off the top of my head, without testing, I see these bugs: buffer = malloc(size); should be *buffer = malloc(size); because buffer is a Open the file. Display the string. } // Read contents from file. Write a c program for concatenation two strings without using string. Here we read the file name and n are specified on the command line. 1. h> #define int main(void) { int len; char line[MAXLINE]; while((len=mgetline(line,MAXLINE))>0) { reverse(line); printf("%s",line); } return 0; } int mgetline(char s[],int lim) { int i,c; for(i=0 May 28, 2013 Here is source code of the C Program to reverse the contents of a file and print it. exit (0);. h> // to count the total number of characters inside the source file long count_characters(FILE *); void main() { int i; long cnt; This program will print the contents of given file name in reverse order. txt"); int p=in. Call strrev, passing the string as an argument. My file-reversal needs. So we need to copy the contents of a file into another file than we will use "fstream", it has functions capable of both reading & writing in the file. c = fgetc (fptr);. Just like tac command in Linux. fptr2 = fopen (filename, "w" );. For my situation I needed to (a) get 10 lines from the end of a file, (b) reverse those ten lines, and (c) save them to another file. Reverse each line of the file, 4. seekg(-i,ios::end); If the file isn't too large, it would be easier to read the entire file contents into memory, reverse them, and then output. if (fptr == NULL). The C program is successfully compiled and run on a Linux system. fptr = fopen (filename, "r" );. The code: printf ( "Enter the filename to open for writing \n" );. h> #include<errno. K = *argv[2] - 48 Step 7: read the item from file C Program to Replace a Specific Line in a Text File - #include int main(void) { FILE *fp1, *fp2; //'filename'is a 40 character string to store filename char filename; char from user and stores in 'filename' scanf("%s", filename); fp1 = fopen(filename, "r"); //open file in read mode c = getc(fp1); //print the contents of file . h>. { char fname[MAXSTRING]; int c;. NOTE: both the text file has to be present initially. If you have a file with this name, you should change its name or move it because when we execute this program, its contents will be erased. h> #include<fstream> using namespace std; main() { char c; int i; ifstream in; in. It is fairly easy, once you understand how lseek works. open("efg. Jun 4, 2011 This program shows how ftell() and fseek() functions are used to move file pointer through a file. fputc (c, fptr2);. Lines beginning with # are processed by the preprocessor before compilation. Here is source code of the C Program to reverse the contents of a file and print it. txt", "r")) { printf("The FILE has been opened\n"); fp2 = fopen("File_2. Nov 20, 2014 The following is a C code for copying the contents of one file in reverse order in another file. */ Code: #include<iostream> #include<conio. The program output is also shown below. // Open another file for writing. c = fgetc (fptr1);. Find code solutions to questions for lab practicals and assignments. /*; * C Program to Reverse the Contents of a File and #include<stdio. FILE *ifp; fprintf( stdin Tags for Reverse a file in C++. Example program for strrev() function in C: In below program, string "Hello" is reversed using Nov 10, 2015 C Programs · Printf and Scanf · Switch Case · If Else · Loops · Series · Arrays · Arrays & Strings · Functions · Function without recurssion · function recurssion · Dynamic Memory Allocation · Structure and Union · File Handling · Command Line Arguments · Data Structure · DSUC_Array · Stack · Queue · Linked Jan 18, 2006 printf("Enter the string to reverse - "); scanf("%s",str);/*Read contents of string*/ reverse(str);/*Function call by using str */ return 0; } reverse(char str[10]) /*Function to reverse string*/ int i,j; char c; j=0; while (str[j]!=' ') /* Total count of characters*/ j++; j--;/*Exact count including Array index 0, reduce total count "fstream" class is used for both reading and writing a file. #include # include #include char buffer[1]; int main() { int fd1,fd2; //the two file descriptors char file1[50],file2[50]; printf("Enter file to reverse: "); gets(file1);… Oct 21, 2014 Step 1: Start Step 2: read the command line arguments Step 3: check if arguments = 3 or not If not print invalid no of arguments Step 4: open source file in read mode Step 5: if NULL pointer, then print file can not be open Step 6: Store no of chars to reverse in k. printf ( "Cannot open file %s \n" , filename);. Code>: Programming & Web Development Community. . return 0;. printf ( "%c" , c);. Syntax for strrev( ) function is given below. Online C Files I/O programs for computer science and information technology students Copy contents of one file to another in reverse order: </Dream. Search: Advanced Program that prints file contents in reverse order. printf ( "Cannot open file \n" );. Display the file, 3. char *strrev(char *string);. K = *argv[2] - 48 Step 7: read the item from file Oct 13, 2011 C Program to reverse the first n characters in a file using the command line arguments. // Open file. The C program is successfully compiled and run on a Linux #include<stdio. Select a text file, 2. If file exists, it reverse the n characters, else it gives the error message. Feb 9, 2015 C program to read a text file and display the contents on screen. txt", "w"); cnt = count_characters(fp1); C Language Source Codes (C programs) - C program to print contents in reverse order of a file (just like TAC command in Linux), TAC command implementation in c, how to implement tac command in c language? C language programs, c language examples, solved c programs, print contents of a file in reverse order in c C Program to create text file & print content of file in reverse order. while (c !=Jun 3, 2016 Working on a Unix system, I just needed to reverse the contents of a file, and thought I'd show how I ended up doing it. this program first takes some text input from user and prints to a file. This header contains information used by the compiler when compiling calls String reverse using strrev in c programming language . h>) in the program. char filename[100], c;. Also note that the input file will be copied in memory entirely. This must be done because stream iterators can't go in reverse, unfortunately. strrev( ) function is non standard function which may not available in standard library in C. h> // to count the total number of characters inside the source file long count_characters(FILE *); void main() { int i; long cnt; char ch, ch1; FILE *fp1, *fp2; if (fp1 = fopen("File_1. h header file. Note that this has nothing to do with file open modes. } printf ( "\nContents I had gotten the program to work when I implemented everything in the main space. May 28, 2013 This C Program reverses the contents of a file and print it. And it seems to me Oct 21, 2014 Step 1: Start Step 2: read the command line arguments Step 3: check if arguments = 3 or not If not print invalid no of arguments Step 4: open source file in read mode Step 5: if NULL pointer, then print file can not be open Step 6: Store no of chars to reverse in k. #define MAXSTRING 100 int main(). In. } Nov 5, 2013 #include<iostream> #include<conio. If you don't have a Prev Next. Online C Files I/O programs for computer science and information technology students pursuing BE, BTech, MCA, MTech, MCS, MSc, BCA, BSc. tellg(); for(i=1;i<p;i++) { in. This program accepts the name of the file to be read as a command line argument. printf ( "Cannot open file %s \n" , filename);. C Program to create text file & print content of file in reverse order. } fclose (fptr);. Write a C program that take input 5 diferent student from user and save them in file using standard io by arranging them in ascending order by name while each record contain Sep 13, 2008 Files handling in C - C programming language can handle files as Stream-oriented data (Text) files and System oriented data (Binary) files. Read more about C Programming Language
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|Het Nederlandse Rode Kruis
|11 jan 2018
|01 feb 2018
|Aantal uren
|Den Haag
We help to strengthen resilience of vulnerable people exposed to hazards and crisis situations and we respond to disasters and conflicts to save lives and alleviate suffering. In the Netherlands with more than thirty thousand volunteers and internationally through technical assistance, supplies and money. Together we act before, during and after disasters to meet the needs and improve the lives of vulnerable people. We do this without regards to nationality, ethnicity, religious beliefs, class or political opinion. Our seven principles guide our humanitarian work: humanity, impartiality, neutrality, independence, voluntary service, unity and universality.
The International Assistance division is responsible for all Netherlands Red Cross (NLRC) international support for victims of disasters and conflicts and for vulnerable people exposed to hazards. The International Assistance division has opened a vacancy for a: Technical advisor – Innovative Approaches in Response Preparedness (Financed for duration of project) 32 h p/w.
Position in the organization:
As Technical advisor you are part of the advisory unit of the NLRC. Your position is in one of the selected partner countries (Uganda, Ethiopia or Kenya) or in The Hague. You report to the respective programme manager and the unit lead of the advisory unit. You will work with 510.global (510), the Red Cross and Red Crescent Climate Centre (RCCC), the British Red Cross (BRC), the NLRC programme manager, PME officer, and field delegates, and the National Societies of the partner countries.
Purpose of the position:
The Technical advisor supports a 5-year multi-stakeholder programme (8 international partners) with an innovative approach. Activities address the institutional capacity of disaster preparedness, focusing on climate change and refugees. The three main approaches include: Forecast based Financing, Cash transfer programming in preparedness, and data preparedness. The programme will be implemented in Ethiopia, Kenya and Uganda.
In close collaboration with the RCRC Climate Centre, IFRC, NLRC 510 team and other technical partners, the technical advisor will ensure synergies between the technical advice provided by 510, RCCC, BRC and Kenyan Red Cross Society (KRCS) and identify additional needs in Response Preparedness required to reach the programme objectives. In addition you function as liaison with other partners of the programme and possibly other relevant stakeholders (within and outside the Movement).
Key roles and responsibilities:
Functie-eisen en profiel
Skills and experience
Ons aanbod
We offer
Informeren en solliciteren
Applications: Please send a letter of motivation in English before 1 February with reference to Rob Vugs by using the following link. The interviews will be conducted in week 6 and week 7 of 2018. For more information about the position contact Suzanne van der Velden, firstname.lastname@example.com. Please note that an assessment is part of the selection process. For more information of the work of the Netherlands Red Cross please go to www.rodekruis.nl and www.rodekruis.tv. NLRC on Facebook: Facebook.com/rodekruis and Twitter: Twitter.com/rodekruis


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Resources - DMR - TYT MD-9600 Issues
The following enumerates issues found with the initial release of the TYT MD-9600 dual-band DMR transceiver:
Obtaining the current Customer Programming Software
The software disc that is included with the radio has a DMR folder, but no MD-9600 CPS is found in this folder. A search of the Analog Portable, D{MR, and Mobile Radio folders also do not contain the MD-9600 CPS.
I incorrectly assumed that the CPS found on the service download page on the TYT web-site would be the most current version of the software. After installing this software, and fully constructing my contact list, I discovered that a newer version of the CPS is available on the downloads page on Grapevine Amateur Radio, where the radio was purchased from, and was downloaded from that location. After installation of v1.18, I opened the code plug that had been created under v1.07 and found that the contact list was empty. DO NOT use versions of the CPS that are earlier than v1.18.
Firmware Update - Instructions & Procedure Do Not Function
The firmware update instructions for version v1.18 firmware, which call for pressing the P1 + Alarm (red key) to turn on the MD-9600, do not function. The radio will not power on with this sequence, and as such, will not blink the display as indicated in the instructions prior to moving to the next step. Further, the user interface screen images contained within the v1.18 operation document do not match the user interface elements actually present in the software. No firmware update was performed due to these issues.
Microphone Not Powered From Switched Power Supply
With the radio turned off, the microphone back-light is enabled, and pressing the PTT will illuminate the PTT LED [which means that the user will need to unplug the microphone to avoid running the vehicle battery down on a mobile installation when the radio is directly powered from the vehicle battery].
Dual-Watch Always Enabled & Apparently Cannot Be Disabled
With a zone programmed with a UHF channel in channel member A and a UHF channel in channel member B, and where both of these channels share the same talk group, and where both repeaters are in range, a speaker icon will toggle back and forth between channel member A and channel member B. I implemented a test zone where channel member A and channel member B are on different repeaters with different talk-groups and verified that the radio is indeed performing a dual-watch mode. There are times when a dual-watch feature is useful, but there are also times when a dual-watch feature is catastrophic. An inability to disable dual-watch mode is not conducive to conducting effective communications. There does not appear to be a control (I may be missing something here, but haven't found one yet), either in the CPS or via the front panel, which allows for the enabling or disabling of a dual-watch feature. The only work around I see is to program the zones so that they only contain channel member A or channel member B but not both channel member A and channel member B. This would defeat some of the utility of having a dual-band radio. In our area, we have a VHF and UHF DMR repeaters, with overlapping coverage, and dual-band operation (without dual-watch) would allow manual roaming by simply switch band when driving out of one coverage of one repeater and into coverage of the other (provided each channel member has the same talk-group), where simply hitting the BAND button would move you to the other repeater. Perhaps this could be addressed in a firmware update.
There is only one volume control, shared by both channel member A and channel member B. This means that it is not possible to have the sub-band at a different audio level than the selected main band. Any conversation on the sub-band may capture the receiver (which is scanning alternately between the channel member A and the channel member B), making it difficult to engage in a QSO on the selected band.
I am hoping that something was missing in user knowledge here, and if so, a correction will be applied to this observation.
Beeps & Tones Uncomfortably Loud
The alert tones emitted from the radio are not well balanced to the volume knob when compared to receive audio. In fact, the tones are extremely loud (uncomfortably so) when the volume is set to provide a comfortable listening level for receive.
Both Bands Must Share The Same Memory Zone
Both the channel member A and channel member B share the same zone and channel index within the zone. It would be extremely useful if the zones could be unique for channel member A and channel member B and if unique channel indexes could be applied to channel member A and channel member B.
Active COS Exits Menu Mode
This issue appears to be present on other TYT radios (observed on both the VHF and UHF version of the MD-380): It is not possible to go into the menu when a signal is present on the receiver. This was evident in the test at item 6 above, where USA 3100 (I know, bad choice) was placed into channel member A with a UHF repeater and a quieter talk-group was placed into channel member B with a VHF repeater. Once USA 3100 / channel member A became active, it was not possible to use the ENT key to enter the menu to change zones. Using the P2 key (programmed for zone selection) did function, however, while the receive signal from USA 3100 was active.
Channels Per Zone (Specification Differs From Reality - Not A Bad Thing)
The specification for the radio indicates that there are 250-zones with 16-channels per zone. Applying a limited number of channels per zone is a construct that is held over from radio architectures that used a 16-position switch for channel selection, and imposes compromises in organizing channels into groupings that are coherent to the user/operator. Simply reorganizing how the channel data is internally structured, where the channel data holds a reference to the parent group, instead of having a fixed array structure where a group has 16-channels, would allow for a group to hold an arbitrary number of channels. A good example of where this is done is with the Icom IC-F5021 and IC-F5061 LMR part 90 radios. With these radios, if you want a zone with 48 channels, or a zone with only 3 channels, you can implement these user defined groupings. This would be of great utility if this capability were brought forward to amateur radio DMR radios. A good example would allow a group to encapsulate a large number of DMR talk-group channels (i.e. more than 16) for a specific repeater to be placed into one group, rather than having to spread these channels across multiple groups. This leaves it to the user to determine the best organizing of channels into groups instead of having a manufacturer imposed constraint that forces the user to adapt a channel set into something that doesn't make quite as much sense as it could. There is no reason to carry hardware imposed constraints forward when the channel selection is performed by either an up/down button set or a rotary encoder.
Interestingly, the actual implementation of the software within the radio differs from the specification, and in a good way. Although the radio is specified as having 16-channels per zone, A single zone was created containing 18 analog FM 2m simplex channels in channel member B. After flashing the CPS code plug to the radio, all 18-channels ARE accessible under channel member B. Channel member A only has 2 70cm simplex channels programmed, so the total channels within the zone (combined channel member A and channel member B) are 20-channels, which is below the 32-channel total that might be inferred from a 16 channel per zone per channel member specification. It is possible that the firmware did implement a reference back to the parent zone and not use an array of a fixed number of elements. Hooray!
Up & Down Buttons Inverted
Pushing the up/down buttons on the microphone inverts the action. For example, pushing the down button when on channel 1 will place the radio on channel 2.
It appears that the software is navigating visually in an up and down manner as applied to a list, where the first entry in the list is channel 1, the second entry is channel 2, etc. Under this type of implementation, the down button advances up a channel. But if you're expecting the buttons to behave as if there are channel numbers for each memory position, the impression is one of inverted behavior.
Good Ergonomics
Ergonomically, the 9600 is much nicer (and easier to use) than my CS800D. At least that is the impression on the bench. Road experience might present a different impression.
However, a remote-head option would be very desirable.
Initial indications are that the radio performance is good. Access to a UHF DMR repeater, located 60-miles away and with no direct line-of-sight path, appears to be very good. A thorough evaluation, based on drive-testing DMR on both UHF/70cm and VHF/2m, is pending.
Received signal strength on a VHF/2m analog FM repeater more than 80-miles away shows very good analog FM VHF/2m receiver performance.
Some Settings Not Preserved Across Power Cycles
The band selection is not preserved across power selections. If you are operating on the bottom band, and power down the radio (e.g. shut-down vehicle), on the next power up cycle, the radio will be active on the top band.
The above observations occurred while using CPS MD9600 version 01.18 with MCU version D 003.027. It is unknown whether a firmware update or documentation update will lead to resolving any of the issues outlined above. As updates become available (presumably with a functional procedure to apply a firmware update), this article will be revised appropriately for any changes in observed behavior of the radio.
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|This web-site is developed, funded and maintained by Ray Montagne (W7CIA)


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With the increase in genomic and transcriptomic data produced by the recent advancements in next generation sequencers and microarrays, it is now easier than ever to conduct large-scale comparative genomic studies for familiar species. However, there are more than ten million species on earth, and the study of all remaining species is not realistic in terms of cost and time. There have been a number of attempts at using microarrays for cross-species hybridization; however, those approaches only utilized the same probes for each species or different probes designed from orthologous genes. To establish easier and cheaper methods for the large-scale comparative genomic study of non-sequenced species, we developed an in vitro homology search array with the aid of a bioinformatic approach to probe design.
To perform large-scale genomic comparisons of non-sequenced species, we chose squid, one of the most intelligent species among Protostomes, for comparison with human genes. We designed a microarray using human single copy genes and conducted microarray experiments with mRNAs extracted from the squid. Multi-copy genes could not be detected using the microarray in this study because their sequence similarity caused cross-hybridization. A search for squid homologous genes among human genes revealed that 68% of the human probes tested showed the expression of squid homolog genes and 95 genes were confirmed to be expressed highly in squid. Functional classification analysis showed that these highly expressed genes comprise DNA binding proteins, which are under pressure of DNA level mutation and, consequently, show high similarity at the nucleotide level.
Our array could detect homologous genes in squids and humans in spite of the distant phylogenic relationships between the species. This experimental method will be useful for identifying homologs in non-sequenced species, for the development of genetic resources and for the collection of information on biodiversity, particularly when using the genome of sibling or closely related species.
The recent development of next-generation sequencers has allowed us to sequence the complete genome of various species easily and rapidly [1,2]. Even though deep sequencing is the fastest and cheapest method to date, the species examined by deep sequencing are still limited to model organisms and species that are medically or commercially important. For example, 36 complete genomes are available among mammals, which occupy only 0.3% of species on the earth, whereas only 16 genomes including 10 fruit-fly genomes are available for insect genome, which comprise more than 50% of all species [3-6]. From the viewpoint of biodiversity, we need to know the genomes of as wide a range of species as possible to allow for environmental protection, to provide material for diversified genetic resources and to promote the basic sciences such as ecology, genetics and evolution [7-9]. For species not currently included in genome projects, it is still possible to determine genes and their sequences by constructing cDNA libraries and cloning with RACE methods. Large-scale genomic studies to better understand biological diversity, and evolutionary systems and mechanisms, however, are not possible via these strategies because they are limited to the use of only a few samples. On the other hand, with the spread of next-generation sequencers across the globe, there has been a rapid increase in the accumulation of DNA sequence data [10], which makes it difficult to undertake traditional bioinformatic analyses such as homology searches. Thus, there is a need to develop new methods for large-scale genomic studies of non-sequenced species. Our aim is not to find all homologous genes between sequences, which is not possible in case where RNA is absent or weak. Indeed, detection of all homologous genes is not possible using microarray methods as such experimental methods tend to result in false positive and true negative estimations. There have been several attempts to examine gene expression profiles using microarray [11-20], but the challenge to search homologs themselves by microarray is unique and novel.
Toward this end, we have developed a novel strategy to pursue large-scale genomic studies using a microarray. As a first step, we tried to identify homologous sequences between species diverged hundreds of millions of years ago. In this study, we selected humans and squids, for a comparison of mammals and cephalopods. We choose these species because though they diverged in the pre-Cambrian period and evolved independently, both acquired elaborate eyes and brains that are remarkable among the two major classes of Bilateral animals; i.e., Deuterostomes and Protostomes [21]. Accordingly, a comparison of genes and gene sets between these species is of particular interest for the understanding of animal evolution. There is no need to conduct in vitro homology searches if both genomes are sequenced, so we have chosen squid as a non-sequenced species. There are, of course, many candidates for such a study; for example, humans vs mice or flies vs mosquitoes, but we sought to test our study in non-sequenced species from scratch, and assess the sensitivity for homology detection between relatively distant species. We will continue work on other pairs once we have obtained reliable results for humans and squids.
To search for homologies between human and pygmy squid genes, we developed a novel in vitro homology search array consisting of probes for human and squid genes. The total number of probes on the array was 11,559 including 7,987 probes designed from human genes and 3,572 probes designed from pygmy squid genes (Figure (Figure1).1). To avoid cross hybridization against very similar probes on the same array, we have selected appropriate region in the coding genes in both species (see Method).
Next, we performed microarray experiments using pygmy squid mRNA. The mRNAs were obtained from the whole embryonic head (head), including the brain and eye, and the remaining portion of the embryo (body) for two purposes; to conduct two independent microarray experiments to test for reliability and to observe the changes in gene expression between different tissues. We found the significant expression of 5,435 and 5,431 probes out of 7,987 human probes in the head and the body of pygmy squids, respectively, using Agilent microarray scanner protocols (Figure (Figure2).2). This indicates that about 68 % of the human probes tested were homologous genes (Figure (Figure22).
It is possible that the number of expressed genes is over-estimated due to hybridization to non-target genes on the microarray even though we used standard scanner protocols and our probes were carefully designed to avoid hybridization to non-target genes. Note that such cross-hybridizations will dramatically reduce expression intensities. We first checked the number of probes for which the intensities were high among human probes and found that the expression levels (probe intensities) of most of these genes were less than 1,000 (1E+03 10bit) expression intensities on a 20bit dynamic range as measured by an Agilent microarray scanner. This result implies that most probes detected non-target genes.
To dismiss this possibility, we carefully checked probes with high intensities. We set the threshold as 1,000 expression intensities and extracted the highly expressed genes having intensities meeting this criterion as potential homologous genes. We then determined 94 and 76 homologs in samples of head and body respectively. Out of the 94 and 76 genes, 27 and 25 genes are already known in the public databases; thus, we can conclude that highly expressed genes in our array are homologous genes. Furthermore, 67 and 51 genes are newly identified homologs (Figure (Figure3,3, Additional Files 1 and 2, based on probe sequence data in Additional file 3).
We then validated whether or not our in vitro homology search array can identify genes already known to be highly conserved between humans and squids. We have checked the expressed genes in squid samples using 3,572 squid probes on the array, and found 1,382 and 1,685 out of 3,572 genes are expressed at intensities higher than the threshold, in head and body respectively. A comparison of squid ESTs from the GenBank against human genes designed on our array showed that 118 genes have a less than 1e-04 e-value in blastn search against human genes, and these were then used for the validation of homologies (Figure (Figure3).3). Out of 118 homologous genes, 27 and 25 genes were detected on our array in head and body, which is equivalent to the average detection coverage of all probes and indicates that our strategy is a useful method for the detection of homologous genes in a query sample (Figure (Figure3)3) and could successfully detect homologous genes on our array.
Not only does our array estimate homologous, but we can also detect expression discrepancies between two different samples; i.e., the squid head and body. It is possible that sequence identity of squid genes against human probes might affect signal intensity in the microarray. However, the same probe with a different signal intensity provides reliable information on changes in gene expression. We identified 15 genes in which expression varied significantly between samples for the squid head and body (Table (Table1).1). All 15 genes were newly identified by our method.
The list of homologous genes indicates that 84% of the genes are function-known in human genome annotation, and GO classification analysis [22,23] suggests that the majorities of the genes are DNA-binding proteins (GO: Molecular Function) or are involved in cellular process and regulation (GO: Biological Process) (Figure (Figure4).4). This seems to be a reasonable result because, even more than 500 million years after the divergence between humans and squids, DNA binding proteins are usually included among highly conserved genes due to selection pressure [24-27]. GO functional composition between head and body was not so different, mainly because these genes are highly conserved between distant species and showed essential gene set conserved at nucleotide level.
Our in vitro homology search array was able to estimate more than five thousand homologous genes in squid and human, which represents 68% of the human genes tested in this study. This indicates that a large-scale genomic comparative homology search is suitable for non-sequenced species. We were able to obtain this result from a single experiment, and by doing further experiments, we expect the number homologous genes identified to increase. As only 85 nucleotides and 9,079 ESTs for pygmy squid have been submitted to GenBank as of May 2010, our approach is a very efficient technique by which to identify homologous genes. Our method will be useful particularly for sibling genome-sequenced species.
Several cross-species microarrays have been designed for various purposes, and a number of papers have provided thoughtful insights into the design and analysis of microarray experiments [11-13]. Other approaches have focused on the genomic and transcriptomic diversity within mammals or primates [14,15]. Specific targets, such as nitrogen availability and toxicogenomics, could be pursued through the use of cross-species arrays [16,17]. Heterologous or duplicated regions are hard target to detect with microarrays because of cross hybridization; however, careful assessment and analysis of those data have been reported recently [18,19]. Unlike these previous studies, our approach has a specialized target, the identification of homologous genes between distant species.
There is a vogue strategy for the large-scale genomics based on next-generation sequencers[20], but the advantage of our method lies in the low experimental costs. Microarray-based experiments are 10 to 20 times cheaper than current next-generation sequencing methods, and this reason alone is sufficient to encourage the application of this method to millions of species to promote the the study of biodiversity. In silico homology search methods, including normal homology searches such as blast, are, on the contrary, powerful tools with which to detect both close and distant homologies, but they require query sequences that are usually only partially available in non-sequenced species.
There are some difficulties in detecting homologous sequences by experimental methods. First, in comparing amino acid sequences, DNA sequences are easily mutated over long periods. We often use homology searches (nucleotide-nucleotide searches) for closely related species, and translated searches of amino acid databases for distantly related species. As it is not possible to perform translated searches in experiments, we can only detect homologous regions if their sequences are well conserved and protected from mutations by slow molecular clocks. We are now assessing the efficiency of hybridization by conducting artificial cross-hybridization microarray experiments (in preparation).
Second, we need to focus on gene coding regions for homology detection because it is likely that intergenic regions are too highly mutated for experimental detection. We, therefore, used mRNAs to search for homologous genes between two different species. In the case of mRNAs, problems still arise in the distribution of gene expression intensities.
Third, probe design in the microarray is also problematic. If probes with similar sequences are used in the same microarray, it is possible to detect expressed genes with two different probes as cross hybridization. To avoid this problem, we have calculated probe sequences in which edit distances are at least 70% different from the most similar probes. We are currently investigating the effect of cross hybridization by designing two different microarrays with a few artificial mutation sites (in preparation). The potential for cross hybridization should be considered carefully when designing microarrays.
Fourth, there is a problem with duplicated genes and gene families. These genes have hundreds of homologous siblings that prevent experimental homology searches due to cross hybridization. To prevent estimating false positive homologs, we have removed duplicated genes from the array. Our method was aimed at detecting homologous genes. The search for homology between species proceeds more efficiently if we focus on single copy genes. Detection of homologs of multi-copy genes would require a different probe design strategy.
We have tested this methods on humans and squid, which are distant from each other in terms of nucleotide sequence conservations. We could still identified 95 homologous genes, and estimated more than 5,400 candidate genes in squid that could have homologs in humans. This indicates that if we apply this method with more probes from humans, we may identify an increased number of homologous genes in squid. This method may also be applied to other species groups such Primates, Rodents, or Diptera (flies), thus allowing larger scale and cheaper genomic comparisons.
We have developed an in vitro homology search array for the estimation of distantly related homologs. It allows the estimation of homologous genes between humans and squids, which diverged more than 500 million years ago. Some genes are, of course, false positive estimations, but the highly expressed genes are thought to be homologous genes. This experimental strategy will be particularly valuable when the explosion of DNA sequences data expected to be produced by next-generation sequencers leads to computational limits on the performance of homology searches.
Japanese pygmy squid, Idiosepius paradoxus (Ortmann, 1881) were captured in the shallow waters along the southern coast of Chita Peninsula in central Honshu, Japan and maintained in a tank at the Ochanomizu University. Spawned egg masses were transferred to a Petri dish and kept at 20°C. To determine the developmental stages of the embryo, the criteria established by Yamamoto (1988) were used [24].
Total RNA extractions were performed by using E.Z.N.A.® Mollusc RNAKit (Omega Bio-Tek Inc., Norcross, GA, USA.) following the manufacturer's instructions. Total RNA samples were extracted from the embryonic head part and the remaining portion of the body of pygmy squid at stage-25. The embryonic head (including eyes and optic lobe) was cut using forceps and collected separately from the remaining body and used for total RNA extraction.
We generated microarray probes for squids, I. paradoxus, using the following procedure. First, we extracted 60 bp candidate probes at intervals of 50bp from each squid genes because the probe length for the Agilent microarray is 60bp. Second, for each candidate probe, we calculated the minimum edit distance between the probe and the all squid genes except the gene containing the candidate probe. With this edit distance, we can avoid cross hybridization between probe and non-target genes. A small distance between the probe and the genes indicates a high possibility of hybridization between them. If the distance was more than eleven and the probe located more than 20bp away from the 3'-end of the gene to which the probe belonged, we regarded the probe as appropriate. We designed the microarray using these probes that meet these criteria. When multiple appropriate probes could be selected from a single gene, we selected the probe closest to the 3'-end of the gene.
As probe sequences for human genes, we utilized original sequences provided by Agilent Technologies. However, the sequences are designed using UCSC Human Genome build Hg 18, which is a slightly out of date human genome assembly. Hence we chose probes whose sequences exist in a single gene on UCSC Human Genome build Hg 19. For squid genes, we collected available EST sequences from Genbank and also designed their probes in the same procedure. To remove the similar probes among the human and squids probes, we furthermore selected probes whose minimum edit distances for I. paradoxus genes were more than eleven. Duplicated genes or multi-copy genes were removed during this protocol. By this procedure, we finally selected 7,937 and 3,572 probes from human and squid respectively.
To perform the comparative analysis between humans and squid, we applied total RNA samples from humans and the pygmy squid onto the custom Agilent microarray 8x15K format. The microarray comprises 11,559 target sequences selected by the process mentioned above. Total RNA from the embryonic head of the pygmy squid and the remaining body portion were labeled and applied separately to the array analysis. Briefly, 0.5 µg of total RNA from each samples was used to synthesize fluorescent-labelled cRNA using Cyanine 3-CTP (CY3c) as described in the manual (Agilent Quick Amp Labeling Kit, one-color). Labeled DNA was hybridized for 16hr at 65°C on the custom array. After hybridization the microarray slides were washed using the standard protocol (Agilent Technologies, USA) and scanned on an Agilent microarray scanner. Data were analyzed using the Agilent Feature Extraction Software (v10.7). The software normalize for any differences in dye signal intensity, then, calculates a reliable log ratio, p-value, and log ratio error for each feature to give a confidence measure in the measured log ratio. Microarray data was submitted to CIBEX.
AO and JS conceived and designed the study. MY performed mRNA extraction and conducted microarray analyses. MF applied the bioinformatics approach to design the array. AO, MF and JS conducted the microarray experiments. AO, MY and JS wrote the paper. All authors discussed the results and commented on the manuscript.
The authors declare that they have no competing interests.
These 67 new homologs were selected because they are detected with high intensities (>1000) in the array.
These 51 new homologs were selected because they are detected with high intensities (>1000) in the array.
Probe Name and Probe sequence were shown in the probe sequence file.
We thank Mr. Takashi Kasugai of the Nagoya Port Foundation for his kind help in collecting the pygmy squid. The super-computing resource was provided by Human Genome Center, Institute of Medical Science, University of Tokyo. This work was supported by a grant from the Japan Science and Technology Agency (JST) to AO.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Tuxedo Black Clearcoat Metallic 2009 Lincoln MKZ FWD 6-Speed Automatic 3.5L V6 DOHC 24V 2009 Lincoln MKZ, POWER MOONROOF-3.5L V6-LOCATED IN OWOSSO, 4D Sedan, 3.5L V6 DOHC 24V, 6-Speed Automatic, FWD, Tuxedo Black Clearcoat Metallic, Dark Charcoal w/Perforated Leather-Trimmed Bucket Seats. Recent Arrival! 28/18 Highway/City MPG
Price may exclude tax, title, tags, governmental fees, any emissions testing charges and any finance charges (if applicable). Unless otherwise stated separately in the vehicle details, price does not include documentation, processing, administrative, closing or similar fees. Current mileage may vary due to test drives and vehicle relocation.


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Agnitum Outpost Pro Security Suite 2008
- Decent firewall and antivirus and antispyware modules
- Lots of pop-up alerts, not a very good spam filter
Bottom Line
Agnitum Outpost Pro Security Suite 2008 remains in essence a top-notch firewall with some security features bolted on — some good, some not so good. Price-wise, it's not a compelling proposition. For the moment, Norton 360 remains the security suite of choice for the non-techie user.
Price$ 49.95 (AUD)
Special Offers
Probably best known as a firewall author, Agnitum has revamped its security bundle, Agnitum Outpost Pro Security Suite 2008 — with mixed results. Still heavily focused on the firewall, Agnitum Outpost Pro Security Suite 2008 includes antimalware, antispam, host, rootkit, Web and transaction protection. It is available in single-user and five user versions.
The most obvious change is the user interface. No longer is there a distinction between Internet and "plug-ins" — this list is replaced by the security modules available. You expand each item in Agnitum Outpost Pro Security Suite 2008's left pane and status details are revealed on the right.
The firewall remains as good as ever, on a par with Zone Alarm. Similarly, Outpost Pro Security Suite's combined antivirus (based on the VirusBuster engine) and antispyware module returns good scores and has won recent certification from the likes of VB100 and ICSA Labs. So no complaints there.
The suite isn't as "smart" as its rivals and can swamp you with pop-up alerts. Although it has a "learn" mode for its firewall and antispam, it still requires a relatively high degree of user intervention compared to the likes of Norton 360, which takes intelligent decisions on fairly arcane matters on behalf of the user.
You can tell the Agnitum Outpost Pro Security Suite 2008 firewall to learn your behaviour over a week and automatically create rules, but this rather dangerously presumes your PC isn't already infected with, say, a Trojan.
You can also get it to scan files really quickly, but to do this it uses the same tricks employed by rootkits and so can trigger a false alert. SmartScan is turned off by default.
Unlike its main rivals, Agnitum Outpost Pro Security Suite 2008 lacks true parental controls and full network security protection. Without doubt, its weakest element is the spam filter. Like many others, it's based on a Bayesian filter that learns to identify spam, but this involves you manually marking spam emails.
You can make the program analyse a collection of spam emails, which is what we did, but then it treated an email we sent ourselves as spam. Even when we specifically marked it as not spam, another self-addressed email we sent ended up in the spam folder. It works only with Microsoft mail clients, too.
A turn-off is the way Agnitum Outpost Pro Security Suite 2008 presents its status results — at times it resembles a spreadsheet of numbers. This may suit some techies, but the average domestic user's eyes will simply glaze over when confronted with things like this.
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Happy Birthday to our little boy! I cannot believe that one year has already gone by and that my baby isn't a little baby anymore!! :(
We had a little party yesterday to celebrate the big year milestone. Der cooked one of Aidan's favourite meals - stirfry - and we had Julie, David, Asher and Liesl and Carey over for supper to help us celebrate. It was a whole lot of fun and very relaxed. Kirsi was sooooo excited to have our guests over and to finally be able to dig into the cake.
Aidan's monkey cake (for our little monkey):
Singing "Happy Birthday":
Taste of cake:
Poke, poke. This is what Aidan always does with food he's never had before.
Heh, heh! This icing is pretty good! Though he wasn't too fond of the cake.
Ooh, icing is fun to smear all over too!
As you can see, he ate some icing and smashed and squished his cake.
Chocolate icing everywhere!
Kirsi helped Aidan open a present (his baby):
Oooh, what's in here?
For more pictures, go to our Photobucket site.
Sunday, June 21, 2009
Aidan's 1st Birthday
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I got POOPED on at Jurong Bird Park & Mass - Singapore Day 3
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|Solitude framed - Psalm 46-10
|Solitude is based upon a photo by David Lorenz Winston. 'Be still and know that I am God. Psalm 46:10.' This piece of art is from our framed and amp; matted textured collection. The print has been mounted onto solid and very durable 3/4" MDF board. A . . .
CATEGORY-> Gifts/Specialty -> Home D?r
STATUS-> instock
Price: 121.00


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Hi, I'm Celina! I'm a recent graduate who is now working full time as a junior designer at a design studio in Richmond. My work keeps me out of the house during the weekdays but I like to keep busy on my weekends with brunch and F45. I do also enjoy a good night out and quiet, relaxing nights in. I'm easy to live with. I'm clean and super handy with a vac. I'm a social person but respect your space and privacy. I'm looking for a friendly home and great people to move in with around Richmond/South Yarra and surrounding suburbs.
I am easygoing and considerate, and also hardworking. I enjoy watching TV shows and generally relaxing in my spare time. I also enjoy getting coffees and brunch on the weekend. I think I would be pretty easy to live with and get along with!
Hi, I'm Yasmin 27 year old nurse from the UK. I'll be arriving in Melbourne on the 28th February, looking for somewhere to live not to far from the city and with good transport links to all the hospitals. Looking for a fully furnished room as I will just be coming with just my backpack. I am an outgoing, fun loving person, I love traveling and enjoy running, yoga, bouldering and relaxing with good food and good friends but I also enjoy my own space. I'm pretty chill, tidy and easy to live with.
Hi all! I'm James. I moved down to Melbourne about 7 months ago, and I'm currently living in an apartment in Southbank. Now I'm comfortable with the city (especially living so close to the cbd), I want to move a little further afield to somewhere like South Yarra or St Kilda or something. I work as a lead designer (graphics, websites, animations, videos, games, apps and more) in the city Monday to Friday (9am to 5:30pm) at a pretty cool company! I'm also a pole dancer (but not a stripper)... I'm looking to share with one other person (male or female), preferably around my age. I like going out, enjoy dinners (cooked at home and eating out), and like to keep a tidy home. I'm looking to share with someone who wouldn't mind hanging out some of the time, but also appreciates their own time too. I come with fridge, super awesome couch, 2 TVs, a few consoles (PS3, PS4, etc), a bookcase and a chest of drawers. I love making cocktails and other drinks. Playing videogames is occasionally a thing that happens. So too are boardgames! Before we move in, I am going to want to get to know you better - I'd prefer if we had some things in common, because hey I wouldn't say no to another friend.
I'm a Drama student from the UK, studying abroad at Monash but looking for a place to stay from mid February to the end of May, somewhere closer to the CBD. (Some flexibility there!!). Sociable, relaxed and respectful, I'm happy to hang out with flatmates or muddle through independently, whatever works. I'll also be working part-time in the CBD and Richmond areas, mostly in the evenings. I enjoy hanging out with a beer and a film, as well as heading to the beach, city, or theatre.
Hej, I am Sandra coming from Austria. I will be joining an exchange program at Melbourne Conservatorium of Music. I am searching for a nice place to stay for at least 6 months. Whenever I have time I like to cook some lovely meals, do yoga, go swimming or make music. I prefer it clean and tidy that´s the reason why I will always clean up after myself. I am very open-minded and friendly, I like to laugh and enjoy nature in my spare time. I do not mind going out for a drink, go to concerts or try some new food but I also like to stay at home someday and spend time on my own. I will respect the privacy of other flatmates but also enjoy socializing and learning about new cultures. I prefer to have an own room in a flat, please funrnished as I cannot bring my furniture from Austria. I would be very happy to hearing from you! SANDRA
I'm really fun to be around, I'm super clean, I love pets and children and I'm a qualified Chef and I work all weekend. I love Golf, AFL, a good wine and great food.
Hello, I'm Chiara, Just moved to Melbourne from Perth. I'll be working in Mount Waverly so looking for an accommodation asap that is within reasonable distance from the Pakenham train line or the Cranbourne train line. I'm a social, extrovert person, I work in architecture and design and I love dancing latin american music. I'd be looking at approx 6 months stay up to one year or longer. I lived in a shared house for the past 5 years in Perth and in Italy before that. Let me know if you'd like to know more.
Hi! I will be moving up from Tasmania to Melbourne to study Animation at RMIT. I'm 21 and have been living in various share houses for around 4 years. I am a tea drinker, I obsessively collect succulents (because I can't kill them) and in my spare time you will find me reading, binging netflix, walking off a food coma, or creating something. I enjoy swimming and being a little sarcastic. I am super clean, apart from a couple of clothing piles, and looking to create a new home. Hope to hear from you soon!
Hello :) We're a young couple looking for a place to live. We're both working in Port Melbourne and really enjoying atmosphere of bay side. We are very easy to live with, respecting personal space, clean and laid back. We're not party animals, but will enjoy occasional glass of wine. We would love a place that's warm and welcoming with nice people. Thanks guys
I am a young (ish) professional from Ireland who is clean, respectful of privacy, but also up for socialising.
We are both a young couple, Both medical professionals and we are studying to sit Exams to finalise our Australian Registration. Very respectful , neat and clean amd fun- loving. Excited to tart a new life in Melbourne! Our friend, Bee, is helping us find a place while we are still overseas.
Just a couple of young whippersnappers, looking for some people to stay with. Were pretty light hearted type of guys that can get along with anyone. Im very driven and my mate likes to party so together we balance each other out. Im looking for people who are studying at Swinburne, interested in business and an aspiring entrepreneur. He is just after a good laugh. Hit me up if your interested!
Clean and tidy, work full time
I am an ED nurse by profession, so live a life controled by shift work. Enjoy going to cafes, movies, keep fit at the gym, passionate about travelling and meeting new people. I enjoy the company of others but equally happy on my own. Easy to get along with and grounded. Have a positive outlook and enjoy life. I'm very respectful of other people and believe that you should treat others the way you wish to be treated yourself. A flatmate/s who likes to hang out and watch a movie and share a glass of wine but someone who is not in your face. Looking for a place to call Home for Upto 6months before I head off to the UK.
Respectful,tidy friendly person from Europe (Slovakia ).
Im a 26 y.o high school maths teacher (currently on school holidays woo hoo). I'm friendly and tidy and I've lived in sharehouses in melb for the past 5years. I like to see friends, have drinks, go out for meals, do activities etc.
Hi there! :) My name is Friederike and I'm a 25-year old literature student from Germany, moving to Melbourne to study Creative Writing at Deakin University! :) I love travelling and getting to know other countries: I've lived and worked as a barista in Auckland, New Zealand for a year after finishing school and also did a work internship in Reykjavík, Iceland last year. Apart from that, I travel and explore the world around me as much as I possibly can! My passions include yoga, all things creative, great coffee, books, vegetarian cooking and baking, listening to and making music (mostly metal and (punk) rock but I'm open-minded!), socialising in general as well as enjoying fresh air and the ocean. :) Concerning living with me, I love a good mix between socialising as flatmates and privacy. I'm quite ambitious about my studies and so I need my daily alone-and-quiet time - but at the same time, I enjoy cooking together/simultaneously and spending time together as flatmates! It's the cosiest thing ever, right? I also like being out and about during my free time and I'm not a "couch potato". When it comes to tidying and cleaning, a clean kitchen and bathroom are an absolute must for me. ;) If you can imagine living with me, drop me a message! I'm looking forward to getting to know you. :) ... oh and one last note: my preferred length of stay is in fact roughly 5 months but this website doesn't offer this option. :D
I am laid back and easy going. This makes me quite easy to get along with. I am also a movie goer and movie nerd.
hey people, I am starting my Masters at UniMelb this February and am looking at either joining an existing sharehouse or starting a new one. If you're keen to start a new place, let me know! I'm originally from Perth, into various hobbies that change all the time, easy going and a pretty decent cook. hit me up if you're interested
Studying double major in Environments at MelbUni, I tend to spend long hours at library. So when I am home, I like to cook or read a bit. Not a party fan nowadays due to my schedule. Also work part time as barista in a coffee shop. Plus I love making people laugh so I am willing to have a healthy social communication with my roommates. Also I like to have a clean house, so I am up to help in household duties.
Hi this is Sally ,originally from Korea live in Australia for 7years(Australian citizen now) . I live in Townsville, North Queensland and I'll move to Melbourne end of Jan. I'll do small business related photography and I'm beautician as well. Im willing to pay rent on time. I'm pretty easygoing, chill, not picky, very clean and tidy, animal lover. I love going out and hang out with friends,travel,hiking,enjoy casual drinking. I bring my friends over sometimes but not big party person. Always clean up afterwards. If you have a rule don't bring guest over please don't contact me. I'm looking for place which is clean, prefer big room , own bathroom, must have a car park, internet available and freindly social share mate. Can hangout together and have a meal sometimes. So looking for someone live with that I can call a friend more than share mate and understanding my broken English:D Thanks.
Heya! I'm a 23 year old Swedish guy. I've been in Australia since mid October and I'm here on a working holiday visa. At the moment I have money saved and don't need to work, but I will look for jobs once I get properly settled. I am a calm and peaceful person. I tend to not take up a lot of space and make too much noise. I'm comfortable with groups of people aswell as going solo. I'm very clean and organised and I like to keep things tidy. I'm an active person, I play ultimate frisbee with a local Melbourne team and I work out a couple times a week. I enjoy chill evenings at home aswell as nights out in the city. My main hobbies and interests are sports, movies, video games, design and travelling! I'm looking for a peaceful place outside the CBD. Cheers :)
moving out of my current space in Feb. Lookinf for a new place to call home