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tag:blogger.com,1999:blog-7487871339000666216.post1384621054688963927..comments2016-03-09T11:32:05.283-05:00Comments on GROGNARDIA: 12,000 A MonthJames Maliszewskihttp://email@example.com:blogger.com,1999:blog-7487871339000666216.post-23160020440598339972010-09-06T05:35:20.666-04:002010-09-06T05:35:20.666-04:00James said: "WotC is even more secretive abou...James said:
"WotC is even more secretive about their sales figures than TSR ever was."

Which is a pity. I live a stone's throw away from WotC, and back around the time Magic: The Gathering hit it big, they were very open about their future plans, business models, etc. Being part of a gaming magazine at the time, they were very interested in showing us around, and showering us with free product, sales figures, and projections.

Bob Dylan had it right: "The times they are a-changing..."steelcaresshttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-21113266876873280092010-09-05T12:21:08.513-04:002010-09-05T12:21:08.513-04:00>>James //If 2001 was anywhere near the lev...>>James

//If 2001 was anywhere near the level of sales in 1982, that'd be an impressive feat! I don't know that it's not the case, but it seems unlikely to me. //

I don't think it's terribly unlikely due to the immense popularity of the Lord of the Rings movies, whose releases were ongoing at that time. Nothing proselytizes for roleplaying games better than successful mainstream fantasy films, and LotR was the first of such since the 80's. It certainly is capable of explaining the spike of D&Ders at my LGS around then.

//BnN, B.A.M., and other booksellers represent a huge piece of that market-share pie, too, but do they sell a lot of them or have dragon hordes of the things sitting in some warehouse?//

I've never had any trouble finding players for 4th edition in my relatively small hometown store. I dunno what this says about their sales in the era of Amazon (and swiping free scans of materials from the Internet, for that matter), but I'm certain that WotC isn't putting out continuous new material for a system that isn't selling at all.Darrinhttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-72878554820596924262010-09-05T10:42:38.540-04:002010-09-05T10:42:38.540-04:00Last year on the Acaeum, Calithena posted a quote ...Last year on the Acaeum, Calithena posted a quote from another summer of 1980 interview with Gygax:

From "It's Only A Game...Or Is It?", Moira Johnston, New West, August 25, 1980:

"Gygax, a former shoe-repairman, insurance underwriter, unpublished novelist and unemployed gaming enthusiast, describes the phenomenal growth of D&D. 'In 1979 we sold a quarter of a million copies of the basic D&D set, a little better than double over 1978. Our gross sales volume has gone from $150,000 in 1975 to $2.5 million in 1979. We expect to double or triple everything this year.' Although FRP games still rank behind electronic games in national popularity, 'D&D is now equal to any boardgame, including Monopoly', says Dana Lombardy, whose games column in Virginia-based Model Retailer magazine has closely monitored the rise of D&D and FRP. The most stunning measure of growth, Lombardy reports, is that 'of a total national FRP games market of $20 million in 1980, TSR will account for more than $5 million of that - at least 25 percent!' FRP wasn't even listed as a games category in 1974."

http://www.acaeum.com/forum/viewtopic.php?t=8598

The "quarter of a million" number for 1979 is in line with the ~12,000 month (240k/year).Zenopushttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-26678645602248039862010-09-04T23:44:01.807-04:002010-09-04T23:44:01.807-04:00Do these figures represent sales to end consumers,...Do these figures represent sales to end consumers, or just distributors/retailers?
 Just from what I've seen at my LGS, some copies of 4e product go to the shelf 'to die'. I think he even still has a couple copies of the PHB that were part of his original order.
BnN, B.A.M., and other booksellers represent a huge piece of that market-share pie, too, but do they sell a lot of them or have dragon hordes of the things sitting in some warehouse?Hawkmansdaddyhttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-89993517078468710672010-09-04T20:20:53.222-04:002010-09-04T20:20:53.222-04:00I do have to wonder how many copies of the 4E rule...I do have to wonder how many copies of the 4E rulebooks WotC moves each month.

A LOT. Just ask them.

More seriously, I don't think we'll ever know that. WotC is even more secretive about their sales figures than TSR ever was and TSR was plenty secretive most of the time. Plus, anything WotC does say is so cloaked in market-speak and dissimulation that it's hard to know what it really means.James Maliszewskihttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-82034751082155431182010-09-04T20:19:04.509-04:002010-09-04T20:19:04.509-04:00I wasn't talking about the Pokemon card game b...I wasn't talking about the Pokemon card game but the Gameboy game itself.

Aha, my mistake. Yes, I see what you're saying now and, as a father of two kids who've played and enjoyed various Pokémon video games, I think you may be on to something. They definitely include lots of aspects of the tabletop RPG experience in electronic form and could well serve as a useful introduction.James Maliszewskihttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-494132008060636282010-09-04T18:28:40.257-04:002010-09-04T18:28:40.257-04:00I do have to wonder how many copies of the 4E rule...I do have to wonder how many copies of the 4E rulebooks WotC moves each month.Will Mistrettahttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-43253907309126448082010-09-04T18:09:20.032-04:002010-09-04T18:09:20.032-04:00I wasn't talking about the Pokemon card game b...I wasn't talking about the Pokemon card game but the Gameboy game itself. Kids have turned to videogame versions of RPGs because it is easier. All the mechanics are contained in the game itself so they don't have to do the math. RPGs in video game form are vastly more popular than any type of oldschool table top RPGs ever where, and for some reason that has made it less "nerdy". Having a Gameboy and playing Pokemon is pretty much considered a standard part of childhood at this point. Playing D&D back in the 80's when I was growing up made me a "geek". I don't know if the watering down of RPG concepts to make it more accepted and mainstream is a good thing or not.Gavinhttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-44184946600870009642010-09-04T17:57:43.194-04:002010-09-04T17:57:43.194-04:00For better or worse, that itch for many that a RPG...For better or worse, that itch for many that a RPG tabletop would hit is filled by the computer/console version.

Absolutely. Many of the guys I started gaming with in 1979 would definitely have chosen video games over RPGs if video games of the sort that exist nowadays had been available back then.James Maliszewskihttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-7583736549355932022010-09-04T17:56:59.601-04:002010-09-04T17:56:59.601-04:00I remember reading something a few years back abou...I remember reading something a few years back about sales of various D&D products, and I'm pretty sure the peak years were 1982 and 2001.

If 2001 was anywhere near the level of sales in 1982, that'd be an impressive feat! I don't know that it's not the case, but it seems unlikely to me. But since WotC doesn't release their numbers and TSR's numbers are almost as unknown, it's hard to know the truth one way or the other.James Maliszewskihttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-50542095494016743602010-09-04T17:43:59.860-04:002010-09-04T17:43:59.860-04:00Ah, found it via Google. It was D20/4e versus D20/...Ah, found it via Google. It was D20/4e versus D20/3e; my memory playing tricks! :DMatthew James Stanhamhttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-84607049592546054292010-09-04T17:39:13.102-04:002010-09-04T17:39:13.102-04:00I believe that 3e sold better than late 2e was sel...I believe that 3e sold better than late 2e was selling and I can certainly believe that. I don't believe it was ever claimed that 3e sold better than any previous edition of D&D.

I had an online exchange on ENWorld with somebody from WotC who confirmed something similar to this. If I were a paid up member I could search my 61 posts and find it...Matthew James Stanhamhttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-69533313269117547732010-09-04T17:05:27.315-04:002010-09-04T17:05:27.315-04:00@Veil: "I remember reading that 3e sold bette...@Veil: "I remember reading that 3e sold better. If that is true, than it goes to show how badly they dropped the ball and lost their momentum to WOW."

No, any time you try to track down that claim it's a widely-spread misunderstanding or obfuscation (depending on one's interpretation).Deltahttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-22653641445205647952010-09-04T15:12:13.041-04:002010-09-04T15:12:13.041-04:00For better or worse, that itch for many that a RPG...For better or worse, that itch for many that a RPG tabletop would hit is filled by the computer/console version.JoeGKushnerhttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-32520384580790394042010-09-04T14:35:50.251-04:002010-09-04T14:35:50.251-04:00Yes, those numbers are pretty impressive. We proba...Yes, those numbers are pretty impressive. We probably won't see that ever again in tabletop rpgs. I can't say I wouldn't like them to become more popular than they are now, the retro-clones in particular. I think there is room for growth, though I certainly don't think it will be anywhere close to where it was in the early 1980s. That said, I think it can grow beyondthe level it now occupies, andIfor one wouldn't be against that.

A friend of mine who is a teacher at a middle-school was able to interest some kids and get a TT game group started. The first few meetings involved several dozen students, though I'm not sure how it is doing now. But he reported many students having played WoW and finding it too limited. Now, that might not mean they would all be picking up Labyrinth Lord, but any movement from the computer to the tabletop in rpgs is good in my opinion.

I remember reading something a few years back about sales of various D&D products, and I'm pretty sure the peak years were 1982 and 2001. IIRC, the article, or blog post, mentioned the Player's Handbook from 1st ed. AD&D and 3rd ed. D&D had comparable sales numbers, and those were the highest selling D&D products.Chrishttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-27846801481312767382010-09-04T12:48:12.089-04:002010-09-04T12:48:12.089-04:00Pokemon...there, I said it. Sure, Pokémon sold ver...Pokemon...there, I said it.

Sure, Pokémon sold very well in its time, as did a lot of other CCGs, but they weren't RPGs. CCGs are a lot more "traditional" as games than are RPGs, so their success doesn't really surprise me. I can certainly imagine other similarly spectacular successes for traditional games, but tabletop RPGs? I just don't think the mass market will ever again go crazy for them as they did 30 years ago.James Maliszewskihttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-1982029274173724442010-09-04T12:45:58.926-04:002010-09-04T12:45:58.926-04:00If I'm not mistaken the 25th anniversary boxed...If I'm not mistaken the 25th anniversary boxed set booklet discusses some print #s of the products it included (as re-prints).

That would be useful information to see!James Maliszewskihttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-33768546311760113012010-09-04T12:45:18.490-04:002010-09-04T12:45:18.490-04:00I remember reading that 3e sold better. I believe ...I remember reading that 3e sold better.

I believe that 3e sold better than late 2e was selling and I can certainly believe that. I don't believe it was ever claimed that 3e sold better than any previous edition of D&D.James Maliszewskihttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-73387387675084292542010-09-04T12:44:14.327-04:002010-09-04T12:44:14.327-04:00And according to Frank Mentzer, his Basic set outs...And according to Frank Mentzer, his Basic set outsold Holmes and Moldvay in '84 or so. Wonder how many units of that they sold?

I've heard that claim too and it may well be true, but I've never seen any hard numbers to back up the claim. But then, except for stuff like this Gygax interview, I never saw any numbers associated with D&D's sales. TSR was always very tight lipped about its sales figures and print runs, which is a pity, because it'd be very interesting to learn which games/products sold best and which sold worst.James Maliszewskihttps://email@example.com:blogger.com,1999:blog-7487871339000666216.post-9798353665409854702010-09-04T11:03:17.281-04:002010-09-04T11:03:17.281-04:00If I'm not mistaken the 25th anniversary boxed...If I'm not mistaken the 25th anniversary boxed set booklet discusses some print #s of the products it included (as re-prints).

I no longer have my 25th set exceopt for the holmes reprint, but IIRC I believe the estimated total run for the Holmes book was 200K plus copies?? Perhaps someone has it and can correct/clarify?

at any rate- It was a great time, and fond memories of the sheer # of RPG products available, and my time as a kid. Moreso I think I miss the sheer amount of creativity that comes with something "new" or that is a "fad". The RPG market these days is completely tapped it seems for "flavor": nothing is all that different or exciting anymore-unless you count rule mechanics. Rarely does something REALLY intrigue me or capture my imagination. Maybe I'm just old, jaded and cynical, IDK.LitrtRdnckhttp://email@example.com:blogger.com,1999:blog-7487871339000666216.post-39504331914617119222010-09-04T09:40:46.482-04:002010-09-04T09:40:46.482-04:00Pokemon...there, I said it.Pokemon...there, I said it.Gavinhttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-69080218481172859122010-09-04T08:14:24.520-04:002010-09-04T08:14:24.520-04:00the times dictated its success. its just like anyt...the times dictated its success. its just like anything that swept through for its phase of consumer dominance and faded. BMX bikes (joe kid on a stingray is a great documentary on this), Skateboarding, etc... both of those have a way of ebbing and flowing with pop culture though as its more marketable with celebrities and "sport"/trick development for new generations to bite into with dollars. these types of games, as we all know, reduced in popularity not only through computers but i would say the Card Game phase that later phased into heavily more pervasive computer gaming. when Magic hit (and all the other companies that had to make games to follow suit), GENCON in Milwaukee was virtually ALL card gamers sitting around the open halls (and even to this day, my local gaming store still holds more Magic nights/events than any other game). yes, there was still a ton of TTers but even as a early teen i could sense how hard the craft of roleplaying was disappearing at a rapid pace. ill never forget that scene.

a lot of people find TT's too tedious and laborious.

point being, yes, its nice to have the niche more closed off for the die hard players and thinkers/writers/artists... but at the same time, without that commercial success of D&D - its hard to imagine where any of this (and a lot of pop culture in general) would be. things are always changing, in flux and never static. even today its ridiculous to think of these games as "old school" or archaic. as long as there are people playing and things within them are changing, and people are forming new creations - theyre still as "alive" and new as ever.akamiamundurahttp://email@example.com:blogger.com,1999:blog-7487871339000666216.post-89877677873791295582010-09-04T06:49:00.741-04:002010-09-04T06:49:00.741-04:00In France, Mega, a sci-fi multidimmensionnal / tim...In France, Mega, a sci-fi multidimmensionnal / timetravel rpg, was released in magazine format at 100,000 copies. there was three successive editions and it attracted a wide range of people to rpg.

Red Box was common enough, as France Loisirs, a very popular by-post bookseller, diffused it widely, as well as B3. That's were I got mine.Nicolas Dessauxhttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-80769298414653282662010-09-04T06:48:54.429-04:002010-09-04T06:48:54.429-04:00I remember reading that 3e sold better. If that is...I remember reading that 3e sold better. If that is true, than it goes to show how badly they dropped the ball and lost their momentum to WOW.Veilhttps://firstname.lastname@example.com:blogger.com,1999:blog-7487871339000666216.post-78389302302376549162010-09-04T06:16:50.909-04:002010-09-04T06:16:50.909-04:00"The Modeller's Nook (never a big place) ..."The Modeller's Nook (never a big place) sold RPGs as a sideline to its military model kits. It also happened to be within spitting distance of the only comic shop in Belfast."

Dark Horizons. Good old Fred and Spike. Where are they now, I wonder?

"In my opinion, Games Workshop dealt it a serious blow by mving from being a games shop to being a Warhammer-only zone."

If TSR had bought over GW, as Gary wanted, it's intriguing what things would be like today.Nagorahttps://firstname.lastname@example.com


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London School of Hygiene and Tropical Medicine
Course Introduction
This course aims to provide people with the knowledge and skills to initiate, develop and oversee mental health policies and/or programmes in low'resource settings, and to conduct and critically evaluate research on global mental health; these skills and knowledge make it possible for students to make valuable contributions in the domains of research, public health, policy and practice as they relate to the discipline of global mental health.
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Core modules include: Fundamentals in global mental health; statistics for epidemiology and population health; principles of psychiatric research and psychiatric epidemiology; platforms of care for mental disorders; scaling'up packages of care.
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The normal minimum entrance qualification is a 2.1 Honours degree or an overseas qualification of an equivalent standard, or a registerable qualification in medicine, appropriate to the programme; applications with an appropriate technical qualification are also welcomed.
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Hi Brains Trust,
I have recently made a discovery that has really surprised me, and wanted to find out if others have had a similar experience.
I have been supplying 6N2P valves for an Amp School that I run here in Sydney and numbers of these amps have been built successfully this year. However, there has been a niggling issue that until now I have been unable to lay to rest. The issue concerns the 6N2P valves used in the amps that students build. For those who are not familiar with this valve, it is effectively a 12AX7 with a 6.3v heater.
A few of the constructed amps have had a weird distortion when the preamp is overdriven - the preamp sounds great when run clean, but when overdriven has had a really nasty sounding distortion that I was able to "fix" by changing a few preamp valves. However, I haven't been really satisfied with this situation, and have sine wave tested a few sample amps and the ugly distortion is clearly evident when the preamp is overloaded. The odd thing is that the valves bias perfectly, and when a 6N2P-EV is substituted, the distortion seems to go away. So I have had a growing pile of "faulty" 6N2P valves that stare at me in the workshop and stir me to think about this issue on a regular basis.
As mentioned, the valves bias fine, however when driven by a sine wave, the signal triangulates (looks like slew rate limiting) and when driven further there will be a breakthrough on the peaks of the signal - no wonder it sounds so nasty :yikes:
Yesterday, I thought maybe I had a bunch of valves that had low emission so I cranked up the heater volts to get an indication of heater current and performance at higher heater volts - made no difference :scratch1:
Anyway, in desperation, I remembered that these valves have a shield between the two triodes - in fact this in common with a heap of similar twin triodes (ECC88, E88CC, 6DJ8 etc) and this shield is brought out to pin 9. Many valves have internal connections brought out to valve pins and normal practice is to leave them unconnected which is what we have done in the Amp School amps. Surely this shield could not have anything to do with this issue?????????????????
Well to cut a very long story short, to my amazement when I shorted the unconnected pin 9 to ground this nasty distortion disappeared!!!!!!!!!!!!!!! And it gets weirder!! When I removed the ground from pin 9 the distortion is still gone! The only way to get the distortion back is to replace the valve with another "faulty" valve from the bin and sine wave drive it again - distortion. Ground pin 9 - clean full sine waves!!
So, has anyone here had similar experiences?? And who is brave to suggest the mechanism by which this could occur?? The best idea that I can come up with is that with all the electrons flying around inside the valve envelope, the shield develops a static voltage buildup and that this somehow affects things. But how on earth can that affect the electron flow within the triode envelope itself???
Your thoughts???
Most probably some tiny residue between anodes on mica insulator caused unwanted feedback.
Just curious, what is the typical procedure for the shield pin? I'm a newbie, and I assumed it would normally be earthed. Is it usually left unconnected?
Thanks Wavebourn for your suggestion about leakage, but this doesn't explain the fact that the valve then works fine after the ground on pin 9 is removed. If it were a feedback issue, the distortion should return when the ground is removed.
What you described was looking like some kind of parasitic feedback. However, I could not open your images, though...
Edit: oh, never mind: that images were kind of residue from Australian guitar forum from which you copied the message...
Interesting! I always grounded pin 9 just because I could - I did not realise I needed to ;)
I've never grounded pin 9 on 6N1 or 6N2 and never had any problems.
In the Aikido pre circuit it talkes about AC grounding with a capacitor. Other audio dual triodes like ECC... do not have a shield. I assume due to the operating frequency. Even stranger that you have batches of "problem" tubes.:rain:
gcwills can you please repost the images we can't get to see them! Thank's
M. Gregg
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What does CFB mean in Toronto Stock Exchange?
This page is about the meanings of the acronym/abbreviation/shorthand CFB in the Business field in general and in the Toronto Stock Exchange terminology in particular.
Chieftain Metals Incorporated (de-listed)
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- The CFB was a British four-wheeled cyclecar made between 1920 to 1921 by the CFB Car Syndicate Ltd of Upper Norwood, London. The company name stood for Charles Frederick Beauvais who later went on to build the Bow-V-Car and then joined coachbuilder New Avon Body Company designing bodies for Standard, Crossley Motors and others. Very few CFBs were made. The 1078 cc, air-cooled, Precision V twin engine with RAC rating of 9 hp was coupled to a friction drive which gave a varying drive ratio by moving the driven wheel along a cone. This then used bevel gerars to connect to a countershaft and thence to the rear wheels by a belt.
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Massimiliano Allegri praised Djamel Mesbah and Stephan El Shaarawy, but has bad news for Milan's Alexander Merkel.
The Rossoneri beat Lazio 3-1 at San Siro to set up a Coppa Italia semi-final against Juventus.
"There were several chances in the first half and Marco Amelia did well to keep Lazio at bay," said the Coach.
"My team did well and we really wanted to reach the semi-final. Hopefully we can keep going and reach the Final, too."
Allegri reshuffled his squad for the Coppa tie, as Mesbah was given his first start in a Milan jersey.
"Mesbah had a good game and defended in a very orderly fashion. El Shaarawy is improving with every match, while Merkel also played well. It's just a shame Merkel picked up that injury and it will be evaluated over the next few days."
German youngster Merkel was brought in to help calm the injury crisis in midfield, but limped off this evening with a sprained left knee.
Catania striker Maxi Lopez was spotted in the stands for this game, fuelling reports he'll complete his transfer within 24 hours.
"As Adriano Galliani said, we will sign either Carlos Tevez or Maxi Lopez."
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Yes hemophiliacs, its 2:30 in the morning and while I am tired I felt like writing. Of course I've been staring at one of my favorite comic runs of all time which sits on top of my book shelf next to some of the best graphic novels of the last 3 decades.
Join me as I discuss Grant Morrison's New X-men. Like it or not the man managed to help save the x-men.
Join me as I discuss Grant Morrison's New X-men. Like it or not the man managed to help save the x-men.
A little backstory before we begin. In early 2001 the first x-men movie was coming out. Right before this the x-books had really not been the flagship title that Joe Quesada wanted it to be.
If memory serves me right it was right after Cyclops had been found (Having just had apocolypse in him mind you) and he and logan were two of the only people left to defend the world against a new form of magneto who had risen in new fashion. The arc had finally wrapped up and we were truly left wondering: What was next?
Grant's first issue hit stands and fans truly were cuaght of guard, no costumes. The team now had streamlined realistic uniforms. Scott and Logan were returning from a mission in genosha with crazy hybrid sentinels. We wre introduced to a very deadly woman who at that time was only known as Cassandra nova. She was cold, calculating and as we soon found out the biological sister to charles xavier.
Speaking of Charles, we were introduced to a new Cerebro now highly advanced technology and renamed cerebra. Every mutant in the world was now trackable. Conducting the experiment with him were two of his closest students Hank Mckoy aka the beast who was rather starting to live up to his name, taking up a very catlike appearance. And Jean Grey who seemed to be more powerful then ever.
But these revelations were soon amped up as Ms. Nova was able to turn the sentinels on the whole of genosha, killing a popultion of over 16 million. only one survivor was found: Emma frost aka the white queen in a new secondary mutation.
Joining Grant for this reinvisioning of the team was constant collaborator Frank Quitely. Who's art was very unique and fitting for the series. Though Frank's speed came into question and we were soon introduced to many different artist's jumping in to the series to help. These artists consisted of many current big names such as Ethan Van Sciver who still to this day wont fully elaborate on what pushed him away from Marvel. But of course we all know he is now recognizable for his Green Lantern work with Geoff Johns.
Also joining the art team were people like Leinil francis yu who's art is currently heating up the summer's big event book Secret Invasion.Even Chris Bachalo who the last couple years has been doing several stints on x-men books. Even current amazing spiderman artist Phil Jimeniz was involved in the project.
There were moments where the jump from different artists was jarring but never to an extant that took you out of the over all story.
Grant was also able to pull in new characters like another weapon x graduate fantomex, the pitiful yet heroic beak, a second angel who's wings were more buggish, the stepford cuckoo's,Xorn who is now infamous and many more.
The shiar returned in force, a riot happened at xavier's, the mutant ppopulation grew to huge numbers, the phoenix force returned, xavier could walk again and Magneto pulled the biggest surprise on the x-men possible.
The series being the x-men still had the key underlying components of what being different meant, the fears of being alone, fighting for what you believe in, and snuck in messages about equality and peace.
And the big ending had time travel where we got to see a post apocalyptic future. 42 issues and truly redefining the x-men for years to come. Astonishing x-men seemed to be a direct follow up as Joss Whedon picked up the toys that grant set in motion for years to come.
Sure some people have a distaste for the run, others hated what seemed to be marvel's complete disrespect for what grant set up with certain characters (like letting magneto stay dead and the whole xorn mess) but what can't be denied is the impact this had on the x-men as a whole. It brought back old fans and just really gave the shot to the system that it really needed.
The Omnibus itself holds up surprisingly well with time, the binding is excellent. There is a forward by Mike Carey who currently writes x-men legacy. The bonus features at the end of the book feature grant's original pitch which makes for one of the most intelligent and interesting dissections of the x-men ever. There's a cover galley, sketch pages and the full run down of the "nuff said" issue in which there was no spoken dialogue.
It's definitely a personal favorite read and I hope others take the time to hunt down a copy of the run and enjoy it for themselves.
Later today I shall be seeing the Incredible Hulk and saturday I finally get to see Indiana Jones. Reviews shall probably follow. I'm not sure what I have planned next (though I really have to plan a day to post my columns, my schedule is way too erratic.)
Posted originally: 2008-06-13 06:34:54


text: Dual Pixels: "Ever wanted to have people turn into weapons for you? Well Project P-100 quirky antics and the Wii-U made one interesting game expereince. In Project P-100 you play as a Viewtiful Joe look alike trying to stop a huge space invasion on the humans, to defeat these creatures you must gather civilians and turn them into heroes yourself."


text:
Re: Keele
In reply to:
Re: Keele
I have just worked on some Keele information that was in my parents things. Check my Family Tree Maker Home page for Keener, Bowden, Mallow, Keele, etc. I don't know if it will help you or not. My father's mother was Laura Bess Keele, daughter of Iveson Rose Keele. They lived in Texas. Wish you well.


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text: Dear all, my name is Stathis Kamperis and I'm proposing the following project as candidate for this year's Google Summer of Code: "Audit, unit testing and improvements of the NetBSD math library". Our math library, which is descendant of Sun's libm (just like most BSDs'), has quite a few deficiencies: 1. Lacks an extensive unit test suite 2. Certain functions are unimplemented, especially the long double versions 3. Lacks proper error handling / floating point exceptions 4. Lacks formal proof of the implemented algorithms (besides the comments and the bibliographic references in the code) 5. Lacks documentation on average time, worst-case time and memory consumption of the implemented algorithms 6. Lacks documentation on rounding modes, error bounds (for some functions the ULPs are mentioned in math(3) but the list isn't complete) NetBSD is being used (at least by a few of us) for scientific computation, where some sort of quality assurance is nonnegotiable. Moreover, a lot of modern dynamic languages sit on top of host's libm implementation. Therefore, whatever glitches the underneath library has, they propagate to upper layers (I bet this argument will touch all Python/Ruby developers out there :P). I don't claim that I am a coding god nor that I will magically turn a mediocre libm into an outstanding one in the time-frame of a summer. There's a lot of scientific papers to read myself regarding floating point arithmetic, and a lot of code to write before starting to 'fix' things. Ideally, I'd like to deliver: 1. A certain number of unit tests for every math function. The ATF would fit nicely here. As part of last year's gSoC, I've written some math test cases, e.g. [1]. Such tests reveal bugs that would otherwise go unnoticed, e.g. [2], [3]. We will target correctness, precision, edge case stressing, error handling and every other requirement of the IEEE 754 standard we will be confronted with. We can even build a database of known-good-results from well established CAS's and match it against the output of our libm. 2. Improvements on existing bits (such as error handling) or implementation for missing functions. 3. Some sort of documentation of the algorithms nature, efficiency, behavior. Regarding the politics. I understand that there is a bias towards 'cool features', which, besides being practical, they are exploitable in terms of marketing ('NetBSD has ZFS, DTrace, virtualized network stack, etc'). And marketing we need, absolutely. Also there's a bias towards projects that have an immediate effect and aren't too much on the experimental side (see also the recent discussion re. the fuzzer gSoC proposal). So here's what I think. If Google decides to sponsor all of our students, fine. No problem. If Google decides to sponsor less students than we have, then this project may be managed as a low priority one. Even though I believe it is as much important as the rest of the things that appear on the NetBSD's projects page, I wouldn't want my proposal to become the Apple of Discord and trigger yet another silly never-ending quarrel in the lists. So this project either makes it in or not. But in both cases, it will NOT block _ANY other cool project_ from being sponsored. I hope I stressed it enough :) I'd be glad to listen to what you people think. Best regards, Stathis Kamperis P.S. I don't include any stuff regarding myself, since this is an informal proposal, it's already long and I don't even know if there's interest on the topic. ____________________ [1] http://gitweb.dragonflybsd.org/~beket/pcca-tests.git/blob_plain/master:/math.h/t_pow.c [2] http://mail-index.netbsd.org/source-changes/2009/08/03/msg223605.html [3] http://sourceware.org/bugzilla/show_bug.cgi?id=10401


text:
Description Usage Format Source References Examples
It has been theorized that developing countries cut down their forests to pay off foreign debt. Data are debt, deforestation, and population from 11 Latin American nations.
A data frame with 11 observations on the following 4 variables.
a character vector indicating the country
debt (millions of dollars)
deforestation (thousands of ha)
population (thousands of people)
Ramsey, F.L. and Schafer, D.W. (2013). The Statistical Sleuth: A Course in Methods of Data Analysis (3rd ed), Cengage Learning.
Gullison, R.R. and Losos, E.C. (1992). The Role of Foreign Debt in Deforestation in Latin America, Conservation Biology 7(1): 140–7.
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.


text:
Joe Smith, husband to aid of State Senator Dean Skelos (R), pled guilty to charges related to his attack on Iraq War Veteran and small business owner Carl Achille, 30 of Elmont.
The stocky, often belligerent Smith is no stranger to the law in that he apparently has sealed arrest records, allegedly drug related. On March 11, 2013, Smith hurled a barrage of racial insults as he bum rushed Achille at a community meeting in the Elmont Public Library.
The meeting held by the Elmont Coalition for Sustainable Development (ECSD) to hear presentations by two alternative developers to the ill conceived minor league soccer stadium proposed for the Belmont Racetrack property. Mr. Achille's question "why did the request for proposal [RFP] deliberately excluding the integration of housing for young professionals" ignited ire from Town of Hempstead employee, Claudine E. Hall, and Smith's wife, Sandra Smith. This seemed the catalyst that provoked the attack. A witness to the incident recalls Hall charging at the young war veteran and being restrained by attendees to the meeting.
For Smith this is the second physical/verbal attack on community residents at the Elmont Public Library. The ECSD has been turned into a political organization and residents familiar with the patronage and outright bullying by members of the group have called for Sandra Smith's resignation from the ECSD board. She has resisted.
Smith, wife Sandra Smith chairs the ECSD board, and is a loyal Republican soldier. Both Smiths are strong supporters of the proposed Cosmos soccer stadium championed by junior state senator, Jack Martins (R) despite broad community objection to the stadium.
Achille applauds the verdict and states that "the verdict sends a message that our community is strong and united and will not stand for thugs and goons hell bent on division, and hate. Community meetings are in place for civil discussion and the advancement of our neighborhood, not violence and ignorance." The attack by Hall and the Smiths on Achille has not made him bitter. He said "I pray for them and their families, and hope that they will use their energy for the good of all Elmont residents."


text:
UPDATE: On Thursday Oct. 8 Apple issued iPhone OS 3.1.2, an update that promises to address several of the glitches described below. Among other things, according to Apple, the update:
- Resolves sporadic issue that may cause iPhone to not wake from sleep
- Resolves intermittent issue that may interrupt cellular network services until restart
- Fixes bug that could cause occasional crash during video streaming
Let us know if this clears things up.
– – – –
"OK, I updated last night without a hitch," wrote Headers22, an iPhone owner from southwestern Missouri, on an Apple (AAPL) discussion board last month. "But now I get random complete shut downs. It has happened twice. In both instances the phone has come back after doing a hard reboot. Anyone else having this problem?"
Apparently some other iPhone owners were having that problem — and more.
Headers22 started the topic Mysterious random total shut downs following 3.1 update on Sept. 10, the day after Apple released iPhone OS 3.1. Less than four weeks later, the topic has filled 128 pages, drawn 1,023 replies and been viewed more than 177,000 times.
Although dozens of folk-remedies and at least one Genius Bar solution have been posted (see below), nobody representing Apple has responded to the hundreds of complaints.
Nor has anyone from the company answered the questions posted in several similar topics:
- iPhone 3GS with fw 3.1 – battery life gets even worse (84 pages, 1,249 replies, 108,514 views)
- 3.1 — Unresponsive iPhone? (26 pages, 385 replies, 44,073 views)
- trouble making calls after 3.1 update (9 pages, 125 replies, 10,676 views)
- Since 3.1. Unable to make calls, text or go online unless I restart iPhone. (6 pages, 82 replies, 5,199 views)
Given that there are more than 30 million iPhones out there, and roughly a quarter of them have been updated to OS 3.1, it's likely that there are far more iPhone owners who aren't experiencing these problems.
But given the numbers of customers involved, and the bad PR they can generate, you might think Apple would step up and offer them a solution — especially if there is one.
The Apple blogs are filled with chatter about the new iMacs, new MacBooks, new Minis and new mice that are said to be coming in a matter of weeks. Would it be too much to ask Apple to clean up its old messes before it creates a bunch of new ones?


text:
For 2013, I resolve to post more. GREs and grad school apps took the better of my time, but I'm glad to say it paid off: I've been accepted to London Met Uni for next year waiting to hear back from more. To celebrate, here are some pictures from my holiday trip to Belgium that ends next week when I visit London (and check out my possible new city?) woohoo!
Sunday, 30 December 2012
Posted by little S at 12/30/2012 No comments
Labels: travel
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text:
Cyber democracy in Madrid: Participation moves online?
Spanish municipal elections signalled significant changes within the local government of the capital. The party Ahora Madrid emerged, taking a third of the seats in the city council as well as the Mayoralty. In order to promote citizen participation in local governance, new online platform "Decide Madrid" was introduced. Has it managed to find a place in the hearts of the rebellious electorate?


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Congress passed the Telephone Communication Protection Act (TCPA) in 1991. The FCC was given the power to interpret it. In July 2015, the FCC released a sweeping TCPA Declaratory Ruling and Order with clarification and new guidance on robocall restrictions. The order cleared up loopholes and strengthened consumer protections already on the books. The FCC's 2015 Order is good for people with cell phones and bad for businesses that repeatedly call cell phones without permission.
The big reason behind the TCPA and the FCC's 2015 Order is the huge number of complaints about 'robocalls' by businesses. These businesses market their products or services by cell phone or faxes to potential consumers and customers.
So what is a robocall?
A "Robocall" is any call that is dialed by an autodialer or answered with a recording or simulated voice. The TCPA prevents robocalls to cell phones.
What is an Autodailer?
The TCPA defines "automatic telephone dialing systems" or "auto-dialers" as "equipment which has the capacity – (A) to store or produce telephone numbers to be called, using a random or sequential number generator; and (B) to dial such numbers." The FCC has interpreted that definition broadly, to mean any dialing equipment that has the capacity to store or produce and dial random or sequential numbers, whether or not the equipment is actually used for that purpose.
Text Messages are "Calls" under the TCPA
The FCC reiterated that SMS text messages are subject to the same rules under the TCPA as voice calls and that Internet-to-Phone text messaging technology is a type of "auto-dialer" under the TCPA. Therefore, calls made using Internet-to-Phone text messaging technology also require consumer consent. You must give prior express written consent to receive text messages.
Prior Express Written Consent is Required
Automated telephone equipment cannot be used to call or send text messages to cell phones unless the caller has the consumer's prior express written consent. If a caller violates this rule a consumer can receive damages for each violating call and message. If a caller willfully or knowingly violates this rule a court can increase the award.
Reassignment of cell phone numbers
A business placing robocalls needs to know the accuracy of the numbers its dialing, especially the cell phone numbers it is calling.
For example, in early 2014, Twitter was subjected to a $5 million class action lawsuit charging that it had violated the TCPA by sending unsolicited text messages to consumers. Twitter had those consumers' consent to contact those phone numbers. However, Twitter did not know how many of these phone numbers in its database had been reassigned to new cell phone owners.
Imagine getting repeated unwelcome calls to someone else on your cell phone. Did you know that when a number is reassigned it is published in a database? All a caller has to do is check the database…or they could listen when you tell them that you are not the person they are calling.
Businesses that engage in high-volume calling must confirm that these phone numbers which includes phone numbers that they have consumers' consent to call using an autodialer or prerecorded message, are still owned by the consenting consumer. Any phone call made or faxes sent to a non-consenting party violates the TCPA.
The difference between "manual" vs "autodial" phone systems
The TCPA restricts the use of "automatic dialing" systems, but not "manual" dialing phone systems. Business owners or managers often mistakenly think that making a "manual dial" call with an "auto-dialer" is simple, when it is not. A manual call is only accomplished when the system that places the call has no association or connection with the business or organization's auto dialer. Whether a call is 'manual' or 'automatic' has nothing to do with the person making the call and what he or she thinks or intends to do. Only the phone system matters, not who the caller is or nature of the particular call.
A Consumer's Right to Enforce the TCPA: Private Right of Action
The TCPA allows a consumer receiving calls to file a lawsuit against the caller alleging a violation of the TCPA and asking the court to either:
(a) enjoin such violation,
(b) seek to recover his or her actual monetary loss from such a violation, or to receive $500 in damages for each such violation, whichever is greater, or
(c) both such actions.
If a court finds that a defendant willfully or knowingly violated the law, the court may increase the amount of the award to an amount equal to not more than three times the amount otherwise available under the law.
Only the "Called Party" can Consent to Receive Calls
At least one court found that the "called party" must be the person who gave consent for the call. For example, a creditor can only call a consumer's relative's cell phone if that relative gave his or her express consent for the creditor to call. A third party cannot give consent for you to receive calls.
When calls to cell phones are the primary means of a debt collectors' communications, then the consumers' request must be considered. When a consumer verbally requests a creditor to stop calling, then that request must be honored. Courts have upheld that the principal that if a consumer tells a creditor to stop calling, then the calls must stop.
Revoking Consent
A robocaller cannot tell a called consumer how to revoke his or her consent. Consumers may revoke their consent through any reasonable means once they no longer desire to receive calls or text messages. This includes verbal communication. If you are getting robocalls, you don't have to go through some complicated procedure to stop the calls. Just telling them to stop over the phone is all that is required. While it may be a good idea to document your revocation with a letter, you are not required to do anything but say "Please stop calling."
Keep in mind that porting a telephone number from wireline service to wireless service does not, by itself revoke prior express consent. A caller is allowed to rely on your prior express written consent until you tell them otherwise.
Oral Revocation of Consent to Call
A 2014 case from the 11th Circuit Court of Appeals, which covers Alabama, Georgia, and Florida involved unwanted phone calls to consumers. In reviewing the case, the Appeals Court made several important rulings related to the TCPA:
The Court noted that the TCPA allows a consumer to orally revoke any "prior express consent" for the creditor to call the consumer. Phone calls to a consumer do not violate the TCPA if the consumer has given the creditor express consent to call him or her. In this case, the consumer expressly told the creditor to "stop calling." The creditor argued that this oral revocation was not sufficient to revoke the consumer's prior written consent to call. Although the lower court agreed with the creditor that oral revocation was not sufficient, on appeal, the Eleventh Circuit Court of Appeals disagreed and reversed the trial court's ruling. The Appellate Court held that the TCPA allows a consumer to revoke consent in writing, and that oral or verbal revocation of consent is sufficient under the TCPA. As a result, the consumer's request for the creditor to "stop calling" was sufficient to effectively revoke any prior written consent.
The FCC's July 2015 took this ruling and made it nationwide. Anyone, anywhere in the US, getting calls to their cellphone can stop them by telling the caller to stop.
Call recipient consent can be granted, rescinded, and/or transferred
Defining the nature of a call recipient's consent can be tricky and uncertain. A recent landmark case, Mais v. Gulf Coast Collection Bureau, showed how tricky and uncertain the nature of a call recipient's consent can be.
When health care industry members seek to gain permission to contact a patient when he or she is incapacitated or recovering they often must rely on family members and others who aren't the party (the patient) who gave his or her consent to be contacted.
The Mais Court clarified a hospital's ability to obtain consent to dial a patient's cell phone using an autodialer or prerecorded message from a patient's authorized representative, and to pass consent on to the patient's collection agency (or other third party).
No Charge is Required for a Call to Violate the TCPA
The Court of Appeals also ruled that the TCPA does not require a consumer to be "charged" for a call to be a violation of the TCPA. Thus, unanswered calls from creditors and calls to a consumer with an "unlimited minutes" plan violate the TCPA.
A business that wants to create a text message marketing campaign must comply with the TCPA, or that business could face hefty fines for SMS spam
Defining Who Initiates the Call: Texting and Calling Apps
The Commission clarified whether, and under what circumstances, the owner of a calling application ("app") can be deemed the party who "initiate(s)" a telephone call and therefore subject to TCPA regulations.
The Commission determined that an app itself does not make or initiate a call when: (1) an individual uses the app to send an automatic text in response to a voicemail; or (2) an individual user chooses to send an invitational text using an app.
However, an app does make or initiate a call when the app "facilitates" the sending of the invitational text message by automatically sending the individual text utilizing a user's contact list without significant input from the user.
Collect Calling Services and Prerecorded or Artificial Voice Messages
The FCC clarified that collect calling service providers that use prerecorded messages on an individual call basis to provide initial information when attempting to connect a collect call to a residential or wireless telephone number may complete the call without prior express consent and not be liable for a TCPA violation. The FCC found that the person who dialed the number, rather than the collect calling service, is the person who initiates the call for purposes of the TCPA.
A Wireless Number in a Contact List Does Not Establish Prior Express Consent
The FCC clarified that the presence of a consumer's wireless number in the contact list of another person's wireless phone does not, on its own, demonstrate consent by the consumer to the receipt of autodialed or prerecorded calls, including texts.
Reassigned Wireless Telephone Numbers
The FCC Order clarified that the TCPA requirements apply to the current subscriber of the wireless number, not the intended recipient of the call. Also, the term "called party" includes both the actual subscriber and the customer using the number.
However, the FCC stated that where a caller makes a call without knowledge of the number reassignment and with a reasonable basis to believe that it has valid consent to make the call, the caller may initiate one call after the reassignment as an opportunity to learn about the reassignment, but the caller must thereafter cease future calls to the new subscriber unless that new subscriber provides proper consent.
One-Time Follow-Up Text Message Allowed
The FCC found that a one-time text message that is sent immediately after a consumer requests a text does not violate the TCPA or FCC rules, as long as it is: (1) requested by the customer; (2) a one-time only message that is sent immediately in response to the request by the customer; and (3) contains nothing more than the information requested by the customer with no other marketing or advertising.
Financial and Healthcare Industry Exemptions
The FCC exempted from the prior express consent requirement certain calls from financial institutions to wireless numbers which specifically concern: (1) the prevention of fraudulent transactions or identity theft; (2) data security breaches; (3) actions a consumer may take to prevent identity theft after a data breach; and (4) calls regarding money transfers. These exemptions, however, are limited to no more than three calls over a three-day period from a single financial institution to the owner of the affected account. They are also subject to restrictions that require including an opt-out mechanism, identification of the calling party, and a prohibition on telemarketing, cross-marketing, solicitation, debt collection or advertising.
The FCC also granted an exemption from the prior express consent requirements for healthcare provider calls for which there is an "exigency" and which have a "healthcare treatment purpose." As with the exemption for financial institutions, the FCC places various restrictions on calls from healthcare providers.
Use of Call-Blocking Technology
The FCC affirmed that nothing in the FTCA or FCC's regulations prohibits carriers or VoIP providers from implementing call-blocking technology to assist consumers in stopping unwanted robocalls. To the extent that a call blocking technology may be flawed, the Commission requires that the provider offer adequate disclosures regarding, for example, the fact that the technology utilized to block calls may inadvertently block wanted calls.


text:
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[lme-module module="market-stats" city="Bishop" state="tx"]


text:
Veterinary questions? Ask a Vet for Answers ASAP
Hi Louise,
Sorry to hear that Tippy is having such trouble.
The thing about abscesses if they haven't ruptured on their own, is that they often need to be manually ruptured and flushed, and then the cat placed on antibiotics to get them to heal. Some will rupture on their own, but there is no way to know for sure. The longer they remain, the greater the risk for permanent damage or starvation.
If you are absolutely unable to get him into a carrier for a trip to your vet, you have a few options:
You can ask your veterinarian for a sedative that you can give him in order to get him there. Since it sounds like he will need to be sedated in order to have the abscess treated, this may be the best bet.
You can purchase or borrow a live animal trap and place it in the garage to trap him for the same purpose.
Depending on your local ordnances, you can sometimes get Animal Control to come by to trap him for you.
If you don't get him in, it is possible that it will rupture and heal on its own, but with it being on the face, I wouldn't recommend taking the chance.
Hope that helps. Please let me know if you have any other questions.
Dr. Paul


text:
- "My shopping habits...
are like my strategic planning at the office; driven by facts and figures in high pressure situations…Rank & Style thinks like I do!" – Kaitlin, Busy Professional, 25
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Simple ethanol precipitation was a disaster and have no idea why! - (Sep/19/2009 )
I would be very grateful if anyone has any ideas that could help me understand what happened to some of my DNA samples, as this was the last step of a long process with some important samples and I'm quite gutted it went wrong (I guess most people know that feeling!).
The samples I precipitated were 6kb PCR products which had been gel extracted using the stratagene gel extraction kit (Gelred used instead of ETBr). There was 20ug (pooled from several pcr reactions) of each sample in 300 ul of elution buffer. Samples had been quantified using a picogreen assay and a small amount run on an agarose gel, which agreed with the pico green and showed nice clear 6kb bands and no degradation following the extraction. So everything appeared fine prior the the ethanol precipitation.
So I added 1/10 vol of 3M sodium acetate pH5.2 (from sigma DNase free) and 2.5 time new total volume ethanol. Mixed by inverting 20 time and left in the -20 for 30 min. Spun for 20 min at max 14000xg. Could see pellet in all the 12 tubes. Carefully poured away supernatant (but kept it separately for each sample and have not thrown it away). Washed with 0.5ml of 75% ethanol (retained that too). Took great care not to disturb pellets and am confident I didn't lose them, as could see them clearly. Allowed them to dry for 5 -10 min, so not over dryed. Added appropriated amount of water to make them 120ng/ul, based on the earlier quantification, using the pico green assay. Heated then to 65C for ten min to dissolve and mixed by flicking the tubes, then on ice.
Then re-assayed them by pico green and now 4 of the 12 samples have only about 4ug of DNA and not the 20ug i expected and the others have between 11 and 15 ug. Ran a small amount on a gel the 4 samples with low DNA yield form two faint bands one 6kb and the other approx 3kb, and a little tiny bit of smear (the other are a single band of the correct 6kb size).
Then checked the concentration of the samples on a nano drop and they all had similar concentrations of about 150ng/ul according to the nanodrop. The 4 samples have 260/280 of 1.55 and the other sample are a bit higher 1.6-1.8.
I can not figure out what happened to 15ug of DNA lost from each of these samples, in one simple ethanol precipitation???
Have tried
1. Re spinning the ethanol supernatants for 2 hours after storing them overnight in the freezer, no pellet. May need more salt if the dna is there?
2. Thought maybe the samples had become single stranded, as then the may not have shown up well with intercalating dyes such as gelred or pico green, but would still by measured on the nanodrop. So with one of the poor samples I heated it to 95 and then cooled it slowing (0.2C per second), hoping it would reanneal, if these was the case. Ran a small bit on a gel and now the two faint bands have gone and there is nothing!
3 Only other ideas are that some sort of chemical, maybe from the gel column, is contaminating the samples and then when I heated the to 65 it destroyed the dna? Or may intercalating dyes can no longer intercalate, because the dna damaged? Not sure why the other samples seem ok.
I think I'm going to have repeat the experiment, but would really like to understand what went wrong.
Thanks in advance for any help.
I vote for the water you have dissolved the pellet in. I wouldn't use water, I'd use TE. Water of low quality can contain lots of things, such as heavy metal ions. At 65C, those things can trash DNA pretty effectively (let alone at 95, as you have seen). You should not need to heat the DNA to dissolve it, especially if it has not dried. Water can also have low pH, which will cause problems in dissolving DNA. The EDTA helps in chelating heavy metal ions.
I would also make sure that all of the DNA was precipitated from the ethanol solution. Add another 20% ethanol to your supernatents, chill for a while, and then spin down. The ethanol at 2.5x may not be quite enough. Also, make sure the wash in 70% ethanol is really 70% ethanol, not a little less, which can happen if some evaporates.
You might consider pellet paint next time you have valuable samples you are precipitating, since it makes the pellet visible and unmistakable.
If I remember right this picogreen measurement is quite temperature dependent, have you measured your samples at an appropriate temperature, i.e. 20°C? Otherwise there might me a great error in the measurements.
I'd add some glycogen to the precipitation, that helps the DNA to precipitate...
And with TE and avoiding 65°C during dissolving again I agree. I use always low TE (10,0.1) and let the DNA dissolve at room temperature.
phage434 on Sep 19 2009, 08:51 PM said:
I would also make sure that all of the DNA was precipitated from the ethanol solution. Add another 20% ethanol to your supernatents, chill for a while, and then spin down. The ethanol at 2.5x may not be quite enough. Also, make sure the wash in 70% ethanol is really 70% ethanol, not a little less, which can happen if some evaporates.
You might consider pellet paint next time you have valuable samples you are precipitating, since it makes the pellet visible and unmistakable.
Thanks for the comments it is good to know others think along the same lines as me. The water was nuclease free water from sigma and I used a new aliquot. I have frequently heated DNA to 65 without problems (and they have been to much higher temperatures many times during the original PCR). Also prior to loading them on the gel I carried out ethanol precipitation on these same samples, to concentrate them and they were fine, the only difference I can think of is that there would have been more salts and other stuff present from the PCR reaction, previously.
Ethanol was 75% and made up freshly and I also tried adding a bit more ethanol too, prior to spinning it again.
Didn't want to add anything else to the samples, as they are to be sent away for deep sequencing and also 20ug of DNA should be easily visible and that was the case. Although one thing I noticed was a slight hint of orange and am wondering if some of the gel red came through. Thanks again for your idea and this at least helps me realise there is no obvious way of savaging them. I have a suspicion that the gel extraction columns may have become compromise and leak something into the sample,when eluted. The gel fragments were quite bit and so were dissolve in a large quantity of gel extraction buffer and so it took several application to get it all on the column.
What I could try is taking a small amount of my sample and mixing it with some other DNA, heating it and seeing if the other DNA is destroyed. This would at least tell me if there was something damaging in my solution. But then if it was been degraded surely i'd get a smear and not two weak bands.
hobglobin on Sep 19 2009, 09:08 PM said:
I'd add some glycogen to the precipitation, that helps the DNA to precipitate...
And with TE and avoiding 65°C during dissolving again I agree. I use always low TE (10,0.1) and let the DNA dissolve at room temperature.
Thanks for the reply. I think the pico green was ok, as standard curve was fine and it was at room temperature, about 20. Also pico green result correlated with the intensity of the bands on the gel. Although neither of these matched the nanodrop readings which suggested all the sample contained similar amounts and lots of DNA, but uv based measurements do tend to overestimate, as any contaminant that contributes to the 260 will make it appear more concentrated than it really is. And the poor sample had the worst ratios.
I will use TE in future, as I agree it is probably safer, but as mentioned above these same samples went through ethanol precipitation by the same method and were fine.
As we say in our lab, it's called research and not just search!
keliang8 on Sep 21 2009, 05:21 AM said:
Hi and thanks, I realise that is often the case, but the stratagene kit seem to return a good yield of 70% when I previously tested it (gel quantification). Besides, I don't believe it was loss during the gel extration as I checked the samples by pico green and agarose gel and they were there and appeared fine, after the gel extraction, prior to the final precipitation.
So, it is still a big mystery
The other thing you have added to the sample is your 3 M NaOAc. I could imagine it has become contaminated with nucleases or heavy ions. If you continue to be interested in this problem, I would suggest trying to precipitate a known amount of some known-good DNA with the same NaOAc and water, followed by washing with the same ethanol (which could also be the source of the problem). I still think it is heavy metals in your water/NaOAc/ethanol. There is a reason for the EDTA in TE.
phage434 on Sep 21 2009, 01:05 PM said:
That would seem likely but the other 8 samples proscessed at the same time with the same solutions were ok, so surely these would be destroyed unless it was a borderline thing. And if it was nucleases or heavy metals then surely there would be a smear. Will use TE in future, but suspect something was damaged on those particular columns which for some reason didn't damage the dna until I ethanol precipitated them and heated them.
Thanks again for your thoughts, especially as I'm keen for this not to happen again.


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What an exciting week with the release of Gavin's fourth book in the Maze series, The Watchman. We wanted to let you have a sneak peak with another excerpt and to let you know about his author central page on Amazon.
Amazon's Author Central Page on Gavin Hill is awesome. Here is the link … check it out! http://www.amazon.com/-/e/B0039ST8SU The photos are of him at one his book signings and doing a radio interview in America plus the back cover of The Watchman. While on this site shop for all of Gavin's books that are just waiting to be delivered to your front door so you can start reading!
Second Excerpt:
I, Razaal, Father Sky and Walker Among Mankind, knew nothing of my evil brother's doing, but I felt great sadness at what I saw below me. I saw husband strike wife and brother kill brother. I saw lies and great pain. Rulers rise and become kings over people, and people turned into slaves to fulfill kingly lusts.
The All Powerful came to me once more and I returned with him to the spiritual glow. He told me my brother was responsible for the things I had seen below me. Radal had changed his name to Pezzarius and Pezzarius would forever be my bitter enemy. He told me Pezzarius would use cunning and stealth to find my weaknesses and I must always be strong and never let down my guard. I was different from my other brothers and sisters, he said, and I had been chosen, groomed and raised to go up against Pezzarius and his devil army. My job would not be an easy one. I would have to go to Pezzarius's world and report back.
I didn't leave immediately. I rested for seven days and seven nights then left with a new brother. The All Powerful told me my new brother would guide me and turn my fears into strength. His name was Courage and he was brave and strong. He, like me, lacked form. On the eighth day, we set off and the All Powerful wished us a hasty return.
It was a long journey and I just followed Courage through the Universe. We often grew tired and found ourselves in need of rest. Courage didn't talk a lot, but I liked him all the same.
"How do you know where we're going? There's nothing but the All Powerful's creation out here," I said on many occasions.
"Trust me, be strong," was always his reply.
We carried on through star system after star system and finally, we reached darkness. Then Courage sat me down and explained to me that we had reached the end of creation.
"You must be brave," he said to me. "I am by your side and I shall lead you to Pezzarius' world. When we arrive, I shall enter you and we shall become as one. I am Courage, and I was created to serve you. I am the cradle that will rock you, the pillar that will keep you standing tall, the water that will bathe you, the air you will breathe and I am the light in your darkness. I shall give you strength when yours is all but gone and I shall help you find your way back to the spiritual glow."
Until next time …. I hope your Monday weather is as perfect and beautiful as it is in Litchfield, Minnesota today!


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Regular price
$ 3,800.00 - Sold
This is a 30x40 original, acrylic painting on a birch wood panel. The sides are painted white and the back is wired and ready to hang without a frame. The price includes shipping within the U.S.A.. Each painting is created and shipped out of Charleston, South Carolina.


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Phalin 100mg Tablet
Molekule India Pvt Ltd
Composition for Phalin 100mg Tablet
Prescription Required
Primarily used for
Seizures, Trigeminal neuralgia
10 tablets in 1 strip
Medicine Overview of Phalin Tablet
Uses of Phalin Tablet
Phalin 100mg Tablet is used in the treatment of seizures and trigeminal neuralgia.
Phalin Tablet side effects
Nystagmus (involuntary eye movement), Double vision, Sleepiness, Anemia, Peripheral neuropathy (tingling and numbness of feet and hand), Gingival hyperplasia, Increased hair growth, Osteoporosis.
How to use Phalin Tablet
Take this medicine in the dose and duration as advised by your doctor. Swallow it as a whole. Do not chew, crush or break it. Phalin 100mg Tablet may be taken with or without food, but it is better to take it at a fixed time.
How Phalin Tablet works
Phalin 100mg Tablet is an antiepileptic medication. It controls seizures or fits by decreasing the abnormal and excessive activity of the nerve cells in the brain.
In Depth Information on Phalin Tablet
Expert advice for Phalin Tablet
- Phalin should be taken at the same time every day to maintain consistent levels in your body.
- Do not stop using Phalin without talking to your doctor, even if you feel better.
- It causes sleepiness or drowsiness. If this happens to you, do not drive or use machinery.
- Avoid consuming alcohol when taking the Phalin, as it may cause excessive sleepiness or drowsiness.
- It may increase blood sugar levels.Inform your doctor if you are taking any medicines to treat diabetes.
- Do not start any medicine including herbal or ayurvedic without telling your doctor. As it may significantly change the Phalin levels.
- Inform your doctor if you have ever been diagnosed with kidney or liver problems.
- Inform your doctor if you are pregnant or planning to conceive or breastfeeding.
Phalin Tablet related warnings
Special precautions for Phalin Tablet
Phalin 100mg Tablet may cause excessive drowsiness and calmness with alcohol.
Phalin 100mg Tablet is unsafe to use during pregnancy.+more
There is positive evidence of human fetal risk, but the benefits from use in pregnant women may be acceptable despite the risk, for example in life-threatening situations. Please consult your doctor....
There is positive evidence of human fetal risk, but the benefits from use in pregnant women may be acceptable despite the risk, for example in life-threatening situations. Please consult your doctor.
Phalin 100mg Tablet is safe to use during lactation. Human+more
studies have shown that either the drug does not pass into the breastmilk in significant amount or is not expected to cause toxicity to the baby....
Phalin 100mg Tablet may make you feel dizzy, sleepy, tired,+more
or decrease alertness. If this happens, do not drive....
Phalin 100mg Tablet is probably safe to use in patients with+more
kidney disease. Limited data available suggests that dose adjustment of Phalin 100mg Tablet may not be needed in these patients. Please consult your doctor....
Phalin 100mg Tablet should be used with caution in patients+more
with liver disease. Dose adjustment of Phalin 100mg Tablet may be needed. Please consult your doctor....
Severely interacts with other drugs like
Barbinol 20mg/5ml Syrup, Cyblex 60mg XR Tablet, Capiibine 500mg Tablet, Gardenal 30mg Tablet
Patient Concerns
Frequently asked questions about Phalin Tablet
Frequently asked questions about Phenytoin
Phalin is used to control seizures (fits) in epilepsy patients and it works by suppressing the abnormal and excessive activity of the nerve cells in the brain.
Phalin is known to cause gingival hyperplasia (enlarged gums). To avoid this, you must maintain a good dental hygiene (taking proper care of your teeth and gums). Please consult your doctor if you have enlarged gums after taking Phalin.
Osteomalacia (softening of the bones) has been associated with long-term (>10 years) use of Phalin by patients with chronic epilepsy. There have been reports of bone disorders including osteopenia (reduced protein and mineral content of bone tissue) and osteoporosis (thinning of the bone) and fractures with Phalin. Please consult your doctor for further information.
Q. Can I take Phalin with aspirin?
Phalin can decrease the levels of aspirin by increasing its metabolism (breakdown) and hence decrease its effect. Please consult your doctor before taking the two medicines together.
Q. Can I take Phalin with ibuprofen?
Phalin can be taken with ibuprofen. No drug-drug interactions have been reported between the two. However, interactions can occur. Please consult your doctor before taking the two medicines together.
Q. Can I take Phalin with paracetamol?
Phalin decreases the level of paracetamol by increasing its metabolism (breakdown). Increased metabolism of paracetamol can increase the levels of substances that can cause liver damage. Please consult your doctor before taking the two medicines together.
Phalin is not a narcotic substance. Phalin belongs to a group of medicines known as anti-epileptic medicines, these medicines are used to treat epilepsy.
Phalin is not a controlled substance under the Controlled Substance Act (CSA). A controlled substance is generally a drug or chemical whose manufacture, possession or use is regulated by a government.
Phalin is not dialyzable by a conventional hemodialysis machine but can be dialyzed by a high permeability type of hemodialysis. To know more about the different types of dialysis and dialysability of Phalin, please consult your doctor.
Phalin is teratogenic. Based on human experience, Phalin is suggested to cause congenital malformations (birth defects) when given during pregnancy. It can cause defects of the heart, brain, fingers and can decrease the growth of the unborn baby.
Cytochrome P450 (CYP450) is an enzyme which causes metabolism (breakdown) of many drugs. Phalin is a CYP450 enzyme inducer (increases the working of CYP450) causing increased breakdown of some medicines given along with Phalin (known as drug-drug interactions). Please consult your doctor before taking any medicines along with Phalin as it may affect the metabolism of the medicines which are metabolized by CYP450 for example Vitamin D.
Phalin is a weakly acidic drug.
Phalin has not been reported to have any addictive potential and it does not show any symptoms of dependence. However, it should be taken for the duration as advised by your doctor.
Phalin does not give you a high.
Phalin does not cause hyponatremia (low sodium levels). However, Phalin is known to cause hypernatremia (high serum sodium level >145 mmol/L).
Phalin is not known to cause QT prolongation (abnormal heart rhythm). However, it can rarely cause abnormal heart rhythms. Please talk to your doctor if you have any abnormal heart rhythm (beats) while taking Phalin.
Thyroid function may get affected while you are taking Phalin, especially in children. Thyroid function should be monitored while taking Phalin. Please consult your doctor if you have hypothyroidism or a history of hypothyroidism.
Phalin has not been reported to cause weight gain. However, weight loss can occur with longer term use of a higher dose of Phalin. Please consult your doctor if you experience weight gain after taking Phalin.
Phalin can make you feel sleepy (sedation, somnolence, and drowsiness). Please consult your doctor if you feel very sleepy after taking Phalin, especially during the first few weeks of treatment or following a dose increase. Talk to your doctor as you may be advised not to drive or use machines until it is established that your ability to perform such activities is not affected.
Phalin does affect birth control. Phalin can decrease the effect of oral contraceptives (birth control pills) which can make the contraceptive (birth control) effect unreliable. Please talk to your doctor if you are asked to take the two medicines together as you may need to use extra protection for birth control.
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text:
Welcome to Wit-Tee T shirt design. We provide digital printing on T shirts and other apparel utilising our state-of-the-art Direct to Garment printing (DTG) technology.
We can print on cotton-based T shirts, polo shirts, and hoodies.
We also provide vinyl printing services onto other materials such as nylon, windbreaker, team sport shirts, etc. Our digital T shirt printing prices in Adelaide are very competitive and we can enthusiastically meet your DTG printing requirements.


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VALLEY STREAM, N.Y. --The suspect under arrest in a violent home invasion and police shootout on Long Island is due in court Wednesday.
Levy Robinson, 47, is charged in the incident, which happened in Valley Stream on Monday night and terrfied a family.
Police say Robinson, of Jamaica, Queens, and another suspect made two adults and four children lie face down on the floor while they ransacked the house.
The first officer on the scene busted in a back door and rescued all six victims. The suspects then opened fire on responding officers.
The second suspect, also from Queens, killed himself on the scene.
No officers were hit, but four needed treatment for trauma.
Get Eyewitness News Delivered
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Story highlights
- Maria Sharapova says she'd been taking meldonium since 2006 and didn't realize it had declared a banned substance
- "I am determined to play tennis again," Sharapova says
- She's lost several commercial endorsements since the revelation
(CNN)Days after admitting she failed a drug test at the Australian Open in January, Maria Sharapova -- long the world's richest female athlete -- posted a Facebook thank you note to her fans.
"In this moment, I am so proud to call you my fans. Within hours of my announcement, you showed me support and loyalty, which I could only expect to hear when someone would be at the top of their profession. I wanted to let you know that your wonderful words put a smile on my face."
The 28-year-old tennis star revealed Monday at a news conference that she'd been taking meldonium since 2006 and didn't realize it was declared a banned substance by the World Anti-Doping Agency at the start of 2016. She knew the drug, she said, by the name mildronate.
Immediately afterward, the International Tennis Federation said on its website that Sharapova would be provisionally banned from March 12 "pending determination of the case." Sharapova faces a ban of four years, although if officials deem she didn't intentionally break the rules, any final suspension could be two years or less.
The five-time grand slam champion tells her fans that she looks forward to continuing her tennis career.
"I'd like to play again and hope to have the chance to do so," she said. "Your messages give me great encouragement. This message isn't anything else but to say thank you. Thank you very much."
She continued, "I am determined to play tennis again, and I hope I will have the chance to do so. I wish I didn't have to go through this, but I do -- and I will."
As the tennis world continues to react to the bombshell development, Rafael Nadal -- who has been forced to deny he has ever committed doping infractions -- didn't absolve Sharapova and said tennis is suffering.
"It's terrible what happened with Maria for the world of sport, for the world of tennis, but you have to respect the rules," the 14-time grand slam winner told CNN. "The sport must be clean, must look clean and in general Maria affects our image in the world of tennis.
"And now she is in trouble, so she is going to a (tribunal) and we'll see what is going to happen, but if she makes negligence, she has to pay for it."
Despite having a huge entourage, which would include an agent, coach, physiotherapist -- and doctor -- Nadal added it was up to Sharapova to know what exactly she was putting in her body.
"You have a team, you believe in your team, but at the end of the day you have to know what you are taking," he said. "So I don't know if it's 100% her responsibility but it's your responsibility."
Novak Djokovic, the men's No. 1, wished Sharapova "all the best."
"I obviously wish her all the best," the Serb told TMZ. " I've known her for a long time. I feel for her with all that's happening and I just hope she gets out of this stronger."
Sharapova's failed drug test came January 26 after she lost to Serena Williams in the quarterfinals. The ITF said the tennis star was charged with an anti-doping violation March 2. Williams, on Tuesday, said Sharapova showed "a lot of courage" for admitting she'd failed a test.
She began taking the drug for a magnesium deficiency and irregular EKGs, which can signal problems with the heart's electrical activity, Sharapova said. Her family also has a history of diabetes, she added, and other medications were prescribed to her.
Sponsors were quick to distance themselves in light of her admission, although only one, the watch maker Tag Heuer, appeared to end their relationship with the star. Head, her racket sponsor, even said Thursday it intends to extend its deal with Sharapova.
Nike has suspended its relationship with the tennis star while the investigation continues.
Automaker Porsche, owned by Volkswagen, said Tuesday it was postponing all planned sponsorship activities with Sharapova.
Danone, which owns Evian, said it was shocked by the news and would closely follow the investigation.
Cosmetics brand Avon declined to comment.


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Small yield of RNA after on column DNAs digestion , Why - (Feb/21/2014 )
Dear All,
I extracted RNA from duck tissue and it was really high quantity 2000 ng/µl by nanodrop. After that I repeated the extraction with on coulmn DNAse digestion and the quantity dramatically dropped down to 120 ng/µl
What is cause, is the Nanodrop measure the RNA and the DNA together ?
-Mohamed 1984-
Yes, it fails to distinguish them (except for the difference in A280/A260 which is minor, and can't be trusted). You may have introduced RNAses in your DNAse treatment.
May be, but if we assume a nice condition, is this is normal that your RNA quantity reduced after DNAse digestion?
-Mohamed 1984-
No. But you can lose RNA in the cleanup afterwards.


text:
Massara, AB and Williams, KA and Yong, JCL and Onischenko, A and Rorison, JM and Penty, RV and White, IH and Galbraith, A and Crump, P and Silver, M (2000) Analysis of the mechanisms for impaired high-temperature, high-speed performance of 1.3 μm InGaAsP lasers. Proceedings of SPIE - The International Society for Optical Engineering, 3944. ISSN 0277-786XFull text not available from this repository.
In this paper, the static and dynamic performance of multi quantum-well (MQW) 1.3 μm InGaAsP Fabry Perot lasers is assessed experimentally and theoretically to identify the mechanisms responsible for impaired high speed performance at elevated temperature. Initially, threshold currents and spontaneous emission spectra are characterized for a range of temperatures from room temperature to 85 °C to indicate a significant increase in non-radiative current contributions. Preliminary estimates are made for the contributions of leakage and Auger recombination rates, found from the dependence of integrated spontaneous emission with carrier density. Drift-diffusion modelling is found to accurately predict the trend of threshold currents over temperature. Using gain modelling good agreement is found between the measured and predicted integrated spontaneous emission intensity. Gain measurements at 85 °C indicate a reduction in RIN frequency to 63% of the 25 °C value which matches well with experimental small signal performance.
|Item Type:
|Div B > Photonics
|Depositing User:
|Cron Job
|Date Deposited:
|17 Jul 2017 19:02
|Last Modified:
|03 Aug 2017 03:18


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Two JUCO defensive linemen join Kentucky Football for 2015 season
LEXINGTON — Just two days before the start of fall camp, the University of Kentucky football team confirmed two big additions from the junior college ranks will join the Wildcats for the 2015 season.
Alvonte Bell and Courtney Miggins are both 3-star defensive linemen at Pearl River City Community College (Mississippi) and have completed the necessary coursework to enroll at Kentucky this fall.
The JUCO teammates are a huge addition — literally and figuratively — to UK's defensive line that lost stars Alvin "Bud" Dupree and Za'Darius Smith to the 2015 NFL Draft.
Bell (6-5, 260 pounds) actually signed with Kentucky out of high school in 2013, but a broken leg and academics caused him to take the JUCO route for two seasons before returning to sign with Kentucky's 2015 class in February.
Rivals.com ranked the Florida native as the No. 24 weakside defensive end in the country in 2013, and 247Sports currently lists him as the No. 14 weakside defensive end in the JUCO ranks.
Miggins (6-5, 285 pounds) spent one season at Pearl City CC, splitting time between the defensive tackle and strongside defensive end positions. 247Sports ranks the Georgia native as the No. 8 JUCO defensive tackle in the country.
KSTV discussed the late additions of Bell and Miggins on Tuesday's show, explaining how their commitments will go a long way in helping secure Kentucky's defensive line depth this season.
Below the Fold
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text:
Willmia (Robinson) Nelson
b. March 29, 1919, Bountiful, Davis, Ut.
d. Sept. 14, 1992, Bountiful, Davis, Ut.
w/o Mansel H. Nelson
(m) Aug. 27, 1949, Mesa, Az.
d/o Joseph Eldridge & Willmia (Brown) Robinson
Site # 04/095
Contributed on 2/2/04 by jlwight
Email This Contributor
Record #: 3741
On this site you can upload gravestone photos, locate ancestors and perform genealogy research. If you have a relative buried in Arizona, we encourage you to upload a digital image using our Submit a Photo page. Contributing to this genealogy archive helps family historians and genealogy researchers locate their relatives and complete their family tree.
Submitted: 2/2/04 • Approved: 3/28/13 • Last Updated: 3/28/13 • R3741-G3740