text: Module Name: pkgsrc Committed By: jlam Date: Sun Jan 11 02:27:09 UTC 2004 Removed Files: pkgsrc/net/samba/patches: patch-aj Log Message: Update net/samba to 3.0.1. Major changes from version 2.2.8anb6 include: * Active Directory support. Samba is able to join a ADS realm as a member server and authenticate using LDAP/Kerberos. * Unicode support. * New, more flexible authentication (passdb) system. * A new "net" command that is similar to the "net" command in Windows. * Samba now negotiates NT-style status32 codes on the wire, which greatly improves error handling. * Better Windows 2K/2K3/XP printing support. * Loadable module support for passdb backends and character sets. * More performant winbindd. * Support for migrating from a Windows NT4 domain to a Samba domain and maintaining user, group, and domain SIDs. * Support for establishing trust relationships with Windows NT4 DCs. * Initial support for a distributed Winbind architecture using an LDAP directory for storing SID-to-uid/gid mappings. * Major updates to the Samba documentation tree. * Full support for client and server SMB signing to ensure compatibility with default Windows 2K3 security settings. * Improvement of ACL mapping features. To generate a diff of this commit: cvs rdiff -r1.1 -r0 pkgsrc/net/samba/patches/patch-aj Please note that diffs are not public domain; they are subject to the copyright notices on the relevant files.


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Over the Rainbow Wrap Up - Episode 2
Another performance episode is in the bag and what a night it was - we saw ballads, rock songs, classics, the addition of backup dancers, and lessons from an adored Broadway star, Kristin Chenoweth.
The Top 9 Dorothys kicked the show off with a gingham-clad rendition of Ding Dong! The Witch Is Dead.
Colleen was the first solo performer, singing Carly Rae Jepsen's 'Call Me Maybe', followed by Cassandra who sang 'Drops of Jupiter' by Train. Colleen may have broken a few hearts when she admitted to Kristin Chenoweth that she struggles with confidence - but you'd never tell based on her performance last night.
Julia performed Demi Lovato's 'Give Your Heart A Break' and Danielle took the house down with a rendition of 'There You'll Be' by Faith Hill.
Jennifer sang the heart-wrenching 'I'll Stand By You', before we changed pace with Stephanie's powerful cover of David Guetta's 'Titanium'.
Jessie took the stage performing Katy Perry's hit, 'Firework'. Jessie got full marks for acting and presence but the judges encouraged her to work on movement and fluidity. AJ then mesmerized the audience with Sarah McLachlan's 'Angel'.
Michelle Bouey from Charlettetown, PEI, closed the night with a lovely version of 'When You Believe' by Whitney Houston and Mariah Carey.
We saw some truly amazing performances all around, but by the end of tonight's results show one more Dorothy will be heading home.
Find out who it is tonight at 8PM/8:30PM NT on CBC.

subset: commoncrawl


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When Disney's Art of Animation Resort opens this summer, its guests will be one of the first to experience the driving force behind Walt Disney World's NextGen project — the room key. Instead of the traditional card key readers that most hotels use, guests at Art of Animation will access their room via RFID. Although some vacation destinations (notably Great Wolf Lodge) have already embraced the magic of RFID for years and have even taken it far beyond the hotel room, this gesture is particularly noteworthy because it signals the start of what we've already known to be coming and it's just the tiniest tip of the largest iceberg this universe has ever seen.
How much of a spotlight will be shown on NextGen (hereinafter referred to by its proper trademarked name, either Magic+ or My Disney Experience /aka/ MDE) when Art of Animation opens up remains to be seen. In its entirety, however, the RFID will be housed in a patented, resizable — and customizable (for a modest upcharge) — wristband known as the MagicBand. The MagicBand won't just simply hold your room key, however. It will be your ticket (you may recall tests earlier this year using RFID as admission media at Epcot), but more importantly, it will be your identity. It will identify you and every thing Walt Disney World knows about you to every one and every thing around you — characters, attractions and even (let's face it) trash cans. Any place Disney Parks wishes to put a proximity reader and some wires will instantly become aware of you and your family.
Add to that how we recently revealed plans for a GPS-aware mobile app that Disney Parks was working on that would combine guests' own photos and PhotoPass to generate a real-time scrapbook that can be shared and interact with friends and family who would tag along on the vacation virtually. However, based on a recent patent application simply titled 'Storytelling Engine,' it appears that the app may simply be more of a proof of concept for a much greater project employing Magic+. Invented by David J. Canora (FL), Robert Swirsky (CA) and Michael J. Gomes (FL), the patent describes how a 'Story Author' (presumably an Imagineer) can create multiple story arcs for various scenarios and how the system would automatically recognize the scenarios, generate a story using a combination of 'custom media' and stock media and produce output that can than be used as an upsell, such as a flip book, photos, video, streaming media, etc.
Several examples are provided throughout the patent application as to how the system may work, but they all share two common elements: attractions are individually aware when a specified guest visits them; and attractions are equipped with still and video cameras throughout to capture photos and video.
In one such example, the 'Story Author' has created templates for 'I Conquered the Mountains.' The parameters for these story arcs requires that a guest visit Space Mountain, Big Thunder Mountain Railroad and Splash Mountain (no specific time range provided). Upon visiting all three attractions at least once, the 'I Conquered the Mountains (for the first time)' story is generated, using photos and video of the guest taken from the actual ride visit and combining them with stock imagery to complete the story which is further personalized with information known about the specific guest (age, gender, etc), all without any explicit input from the guest. Should the guest visit each of the three attractions at least twice, it would then generate an entirely different story arc using the template of 'I Conquered the Mountains (yet again).' These templates could be applied to any number and combination of attractions and any number of attraction-independent potential influencers such as first visit, time of day, time of year, holiday overlays and birthdays. Did we say birthdays? Indeed we did. Equipped with your MagicBand, those birthday and first time buttons will be purely for show (and the benefit of other guests) as anyone and anything equipped with a reader will instantly be aware of it.
In short, Disney will no longer be able to keep track of just how many times you've been to the Resort, but how many times you've visited each park, each attraction, eaten popcorn, and much, much more.


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NEW OPEN STUDIO for Ceramics or Painting on Canvas !
Ceramics painting is great fun for all ages. We have lots of samples and ideas. Our dassortment of bisque and paints ensure that you'll find just the right project to create. Our friendly staff will teach you the best tips to create a great piece and fire your pottery. This is an amazing activity is for all ages!
Price: $8 Painting fee and plus the ceramic piece.
Painting on Canvas or Painting on Wine glass:
BYOB (Bring Your Own Bottle) and get a small (11×14) canvas for only $24 and full access to all of our paints/brushes! Get inspired to create your own painting. Please note, there will be an artist in the studio to assist with paints/colors but they will NOT be guiding you step-by-step as they do in our regular classes. This session is self-guided.
Painting on Canvas:$24 . Painting one wine glass: $25 and tw0 $35
Easy, come and paint between 1pm- 4pm!
If you want reserve your spot, just pay $8 per person and at the studio you pay the balance accordingly to the activity that you choose.
Kids under 14 has to be with an adult. If you have any questions, please call us at 786.272.5412
If you have a deal for this Ceramics and you want to reserve your spot please, send an email to email@example.com with your name and voucher number. Only first time customers can use a deal from Groupon or Living Social.


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There are places in the Caribbean with high altitude terrain. Within these areas you will definitely find hillside farming, terracing and the like. Well here is a technology that can be quite effective. Its called Fog collectors – These are vertical panels of polyethylene mesh that collect water from the surrounding fog, in high altitude areas and channels it to a storage tank. Thereby water that may be difficult to access because of the terrain, is easily farmed from the sky!
This is a novel idea and as you'll see at this video ( http://www.youtube.com/watch?v=njRtL8lN2b4) provides much valuable water for communities and its agriculture. You can get more information at http://www.fogquest.org/


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There's really not much to the story because it reads like one of the chapters from the novels but it says a whole lot about the series in general. It has the key elements in it despite it being a short story.
- It establishes that Atticus is the last Druid on Earth.
- Despite the notions of Druids being weak in battle, Atticus can hold up his own. He does this by combining physical prowess, battle strategy and some Druid magic.
- He can shape shift.
- He has a very close relationship with his hound, Oberon.
- Trouble always finds him.
This one was a good short read and it's free to download. It does a good job in acting like a filler in-between books.
Technorati Tags: Clan Rathskeller,Book Review,Iron Druid Chronicles,Kevin Hearne


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LAW Writer® Ohio Laws and Rules
If a child support enforcement agency sends a notice imposing a withholding or deduction requirement or any other appropriate requirement to a person described in section 3121.03 of the Revised Code and the payor or financial institution sent the withholding, deduction, or other appropriate notice fails to comply with the notice, the agency shall request that the court issue a court order requiring the payor or financial institution to comply immediately with the notice or be held in contempt of court. If the court issues the order and the payor or financial institution does not immediately comply with the notice, it is in contempt of court.
Effective Date: 03-22-2001 .


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Dionisis Chiotis is a Greek International Goalkeeper who started his career at AEK Athens in 1994 and he stayed there until 2007.
From 1998 until 2000, he had loan spells at Ethnikos Piraeus and Proodeftiki, and when he came back, he became the first-choice Goalkeeper of AEK.
In 2005, he lost his place in the team to Stefano Sorrentino and in the Summer of 2007 he signed for Kerkyra, where he stayed only for one year.
In the Summer of 2008, Dionisis signed for APOEL and he went on to become a legend for the club.
Not only did he won numerous Championships and Cups at APOEL, but he was named Man of the Match in many Champions League games, including a 0-0 draw away at Atletico Madrid in 2009 and he saved two penalties against Lyon in 2012, to send APOEL to the Quarter-Finals of the Champions League.
He shared the Number one Goalkeeper's gloves with Urko Pardo in the 2011-12 Season, and from 2012-13 onwards, he lost his place to Urko Pardo.
However, he still gets a good amount of games each Season, including the Cup Final of 2013-14, and he shows that he can still play at the top level.


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Do what your heart desires!
Is this weird?
Started by ddiinnyyeell, Feb 10 2009 03:17 PM
12 replies to this topic
Posted 01 March 2009 - 03:27 AM
4/1/11 in St. Kitts at the Marriott I married my best-friend!!!
Posted 01 March 2009 - 03:34 PM
| Originally Posted by ddiinnyyeell
Thanks for all the advice! The girls actually came to me and picked their own maid of honor based on who was responsible and available to do it. Saved me a lot of stress!
Posted 01 March 2009 - 04:36 PM
Yeah don't bother its not worth the hassle
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No, that's not a particularly fine Old Welsh name!
It's the Welsh for "inspiration everywhere."
You see, this week, Small Child and I ran away to Pembrokeshire in South Wales.
And while enjoying the fresh sea air, and exploring the pituresque little towns and magnificent ruins, I encountered plenty of interesting names and name inspiration. These are just some of my favorites:
Arawn — We passed one very pleasant day of our holiday painting pottery in a café in magical Narberth, the Arberth of the First Branch of the Mabinogion, and home to Pwyll, from where he rode forth hunting one day and encountered Arawn, Lord of Annwfn — the Celtic Otherworld. There's rather an inspiring and spine-tingling view of the surrounding countryside from the not-so inspiring and spine-tinging car-park in the centre of Narberth; it's easy to imagine the hounds and horses of Pwyll and Arawn hurtling across the fields… Some postulate that Arawn derives from biblical Aaron. I say that's utter nonsense. It is far more likely to derive from the Common Celtic *ar-yo– "to plough" or the same ancient root which gives Modern Welsh: aran "mound."
Carew — One of the most romantic castles I know, a mixture of medieval and Elizabethan. It's name derives from the Welsh, and is equally romantic: caer "castle" and "fort" + rhiw "ice."
Cawdor — Although in most people's minds, Cawdor is firmly associated with Scotland (and, indeed, Macbeth, erstwhile Earl of Cawdor), more recent Earls of Cawdor had their seat at Stackpole in Pembrokeshire. It was a late eighteenth century Baron Cawdor, indeed, who repelled the last invasion of Britain, by the French in 1797 at Fishguard. The Gaelic form of Cawdor is Caladar, deriving from coille "wood" and dur "water" or "oak." An old form was Calder.
Elidor — We stayed in the marvelously named Stackpole Elidor (a name which would have been right at home in Harry Potter!). Elidor is actually an Old Welsh name, and Stackpole is named in honor of a very shadowy saint of the name. As Elidurus, it occurs as the name of a legendary king of Britain in Geoffrey of Monmouth's Historia Regum Britanniae (c. 1136). Its meaning isn't all that clear; while the second element is almost certainly the Welsh dur "steel," the first is fuzzier. It died out as a name in the Middle Ages, but was revived in the 19th. Other medieval variants include Elidyr and Elidir, as borne by the medieval Welsh poet Elidir Sais (c. 1190–c. 1240). It's probably best known today, however, from Alan Garner's 1965 fantasy novel of the name.
Gwri — Gwri Wallt Euryn "Gwri of the Golden Hair" is the name Lord Teyrnon gives to Pwyll and Rhiannon's son Pryderi when he finds the child in his stables and adopts him as his own. It probably derives from the same source as Welsh gŵr "man."
Lyd, Lud — The intriguingly named Lydstep was just down the coast from our cottage. An earlier form was Ludsopp, meaning "Lud's refuge."
Merrion — A tiny hamlet near Stackpole, with a very pretty name. It may derive from the same source as the Welsh boy's name Meirion, i.e. the Latin Marianus "belonging to Marius" or Marinus "of the sea." Given its location, it's tempting to lean towards the latter, and it is perfectly possible that actually, at its heart, is Welsh môr "sea."
Middleton — I can't help wondering if the National Botanic Garden of Wales is rather kicking itself now for changing its name from Middleton, now that the name has been made so famous by the new Duchess of Cambridge. Its original name was in honor of the Middleton family of Oswestry, who built the first mansion on the site that was to become the gardens. It's meaning is straightforward; "middle" + tūn "enclosure," "farmstead," "estate," "manor," "village." We had a rather wet day at the gardens, on this occasion, sadly, though Small Child thoroughly enjoyed the exhibition on mushrooms :).
Myrddin — The Welsh name for Merlin, and the name behind the town of Carmarthen and its Welsh form, Caerfyrddin. We didn't do much there this time, except pop into Morrison's on the way to the Botanic Gardens. I'm going to feature Merlin as a pick of the week in the not too dim and distant, so I'll say no more for now.
Oriel — Orielton is another little Pembrokeshire village near Stackpole Elidor. I don't know its etymology, but the "Oriel" part lept out. Oriel was found as a girl's name in medieval times, a vernacular form of the Germanic Aurildis, meaning "fire battle." It was unrelated to the word "oriel" used of a large, projecting recess with window, often found in a lord of the manor's solar (private sitting room). It was this type of Oriel that gave it's name to the Oxford College.
Pryderi — The birth name of Pwyll and Rhiannon's son, born at Narberth (see Arawn above). He is generally considered to be one and the same as the Mabon — the "divine son" of Welsh mythology, who gives his name to the Mabinogion. It is generally derived from the Welsh pryderu "to take pains" and "to be anxious."
Pwyll — Another Narberth inspiration; the name of the noble Lord of Dyfed, who had his palace at Narberth. Not the easiest of names to say, sadly, but it does have such rich association and meaning; in Middle Welsh, this was "spirit" and "reason," while in Modern Welsh it now carries the meanings of "discretion" and "steadiness."
Rhiannon — Pwyll's wife (and later the wife of Manawydan). She is perhaps the most significant of the figures associated with Narberth; in the Mabinogion, she is an otherworldly maid, who rides a white horse… many equate her with the Gaulish Goddess Epona, and her Brythonic name has been reconstructed as Rigantona from Common Celtic: *r-gan- "queen." Like Merlin, she's on the cards for a post of her own…
Tudor — One of the highlights of our trip was a revisit to Pembroke Castle, birthplace of the founder of the Tudor dynasty, Henry VII. His surname is the Anglicized form of the Welsh Anglicized form Tudur, an ancient name, deriving from the Common Celtic *towtƒ "people" and "tribe" (this became tut in Middle Welsh and also acquired the meaning "country") + *r-g- "king." It was found in Gaul in the Roman period as Teutorix, and is cognate with the Germanic Theodoric.
Twynnell — St Twynnell was another local village, named after its shadowy saint. At the heart of this beauty almost certainly lies the Great Welsh Goddess Dôn, with Twynnell a combination of Dwyn (a variant of Dôn) and gell, literally "yellow," but also "bright" and "shining."


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Albert Biermann is the latest BMW casualty and he is a big one considering he was the vice president of the M division. He is the guy who said he wanted the M division to produce an M7 to rival the Mercedes S63/S65 AMG. He is also the guy who publicly admitted the new turbo BMW motors simply do not match the response of the previous naturally aspirated motors.
He also kept the idea of an E89 Z4M alive stating it hurt not having one in the lineup. Perhaps the biggest and most important development was that he was the one pushing hard for an M version of the i8 despite the other execs saying that 'M' and 'i' must be kept separate.
BMW is clearly having an internal power struggle and identity crisis. You have the head of BMW Norbert Reithofer saying there should be no i8M or M8 and the head of BMW M saying they should have one. All we know is the bean counters keep winning and the enthusiasts keep leaving BMW.
So after three decades at BMW Biermann is gone for Hyundai of all places. If Hyundai is more inclined to produce performance cars than BMW something is very, very wrong.
Biermanns last role was as Vice President Engineering at BMW M Automobiles, responsible for dynamics of acclaimed road and race cars
Hyundai Motor Group appoints Albert Biermann as
Head of Vehicle Test & High Performance Development
Hyundai Motor Group appoints Albert Biermann as
Head of Vehicle Test & High Performance Development
Takes on responsibility for new high performance vehicles and technologies
Appointment is part of a drive to develop Hyundai and Kia vehicles that lead their segments for rewarding driving dynamics
December 22, 2014 - Hyundai Motor Group has appointed Albert Biermann as its new Head of Vehicle Test & High Performance Development.
Based at the companys R&D Center in Namyang, Korea, Mr. Biermann will lead the development of new high performance Hyundai and Kia models, as well as oversee engineering projects relating to ride and handling, safety, reliability, and the mitigation of NVH (noise, vibration and harshness).
His appointment will accelerate the roll-out of new high-performance vehicles and technologies, as well as help to improve the dynamic capabilities of cars across the range. The company intends to become a technical leader in ride and handling, producing vehicles that lead their respective segments for driver engagement. Hyundai Motors involvement in the World Rally Championship (WRC) should also benefit, thanks to increased sharing of performance technologies and insights between engineering programmes for the road and rally stage.
Mr. Biermann, 57 years old, joins Hyundai Motor Group from 1 April 2015 and brings over 30 years of experience in the development of high-performance vehicles, most recently in his current role as Vice President Engineering BMW M Automobiles and BMW Individual at BMW M GmbH, Germany, a position he has held since 2008. He first joined BMW in 1983 and has played a key part in honing the performance and dynamics of some of the marques most acclaimed road and race cars.
Biermann said, I couldnt be more excited to join Hyundai Motor Group. It is a company I have watched closely as it has taken on its key competitors and quickly established a strong customer base in Europe. 2015 is a key year for the brand with the launch of many all-new models and the start of its second season in WRC, so I am really looking forward to the challenge.
Woong Chul Yang, Vice Chairman of Hyundai Motor Company and Head of Hyundai-Kia R&D Center, added, This is a senior engineering appointment and an important part of our effort to further optimize the dynamics of our vehicles, providing even greater rewards for keen drivers. Mr. Biermann has unrivalled credentials in this field and his expertise and experience will help us bring forward the development of new Hyundai and Kia cars that are great to drive, as well as speed the transfer of innovative technologies and insights between our road-car and motorsport engineering teams.
Earlier in 2014 Hyundai Motor opened its brand new European Test Centre at the Nürburgring in Germany, allowing the company to test more effectively and accurately the reliability and drivability of new vehicles.


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Academic Coordinators
Chair of Department
- Professor Adrian Vickers (Acting)
Please direct all Honours, postgraduate research and international exchange inquiries to the Chair of Department.
Unit Coordinators
- Unit coordinators are listed on undergraduate semester timetables.
Unit coordinators (as well as the Faculty) should be informed of any illness or other misadventure that leads students to miss classes and tutorials or be late with assignments.


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I am trading a level 31 shiny machamp gentle nature. The stats are:
Hp 105
Att 106
Def 56
Speed 46
I have begun to EV train it in attack. I would like a shiny onix but I am open to all offers. Please PM your offer.
Diamond Friend Code:
Code:0945 8710 1011
Lv. 53 Machoke. Anyone want? Please PM with offers.
Name is YAZMI (weird name)
FC is 1160-6716-3765
i am looking for a machamp that has over 220 speed if have pm me will trade a shiny camprupt for
Trading shiny machamp, looking for shiny magby/magmar or vulpix or better shinies..PM me for detail
Completed National Pokedex
CLICK HERE FOR Shinies/legend/items
Breeding Service
Email to me or msn if i lag here very seriously
trading a lv.80 machamp pm with offers
Spoiler:- My Shinies::
First banner was over 46800 pixels limit, so Glaceon froze it off. Read the signature rules.
I am trading a level 58 Shiny Machamp WITH Pokerus^^ It was caught as a Machoke in Stark Mountain at level 58. Basically untouched. My 10 year old sister gave it to me, so I know its legit. LONELY Nature. It is a FEMALE also.
Attk: 190
Defense: 90
Sp.Attk: 97
Sp.Def: 119
Speed: 71
Ability: Guts
Wake-Up Slap
Cross Chop
Scary Face
Dynamic Punch
PM me for offers. An instant win for untouched Shiny Starters, more likely for a shiny Treeko or Charmander.
Also will accept a shiny Miltank.
PM me your FC if you want to trade, battle, ect.
Would like a Manaphy, Deoxys, Darkrai (LEGIT), Shiny Latios, Shiny Beldum
PM me...
I have a (All shinies): Doduo, Vigoroth, Chansey, Delcatty, Gloom, Staryu. Ledian, Buizel, and a Miltank. Most have been leveled up a bit...
lookin for some trade-backs!
pokemon to trade-back
please pm anytime!
lookin for some trade-backs!
pokemon to trade-back
please pm anytime!
i hav these pokemon and i can breed a varitety of these pokemon and will trade them 2 anyone interested
legit naughty nature EV trained shiny machamp for trade, i am looking for shiny starmie
legit naughty nature EV trained shiny machamp for trade, i am looking for shiny starmie
i have 2 untrained adamant machops. pm me for them.
I have a lvl 100 Machamp. Will trade for shinies or Darkai.
Hey, I am looking to evolve my Machoke to get a Machamp. Can anyone help me? PM if you can. Thanks.
i have a shiny untouched machamp i search av untouced
latios whit timid nature or a untouched bronZOR pm me if your interestid
sassy nature sorry for the spam
Have Lv 39 Machoke in need of trade for evolutionary purposes. PM me if you want to help.
EDIT: Complete, thank you MewSweetie
Last edited by Kinrah; 14th August 2007 at 3:56 PM.
It seems there's a new villain around...
(image courtesy of http://www.mzzt.net/tf2/)
I have an untouched lv45 Shiny Machoke. Looking for Shiny Ralts or Shiny Shuckle with a cool nickname. I'll also accept offers of other shinies because I'm not entirely sure what I want. PM, IM or E-mail me.
>> Current Anime Watch List <<
>> Pokemon & Items Trade List <<
I just caught a level 13 Naive Shiny Machop.
Not sure what I want but feel free to pm me for a trade if you want to offer
i have a few egg move mochop with all the punches on him they are lv 1 pm me for offers
hey everbody add me on d/p
fc: 4682-4960-4423
i luv dis guy 4 somereason
Offering a LV: 47 Machoke. CALM nature. FEMALE gender.
Cross Chop
Rock Smash
Focus Blast
Offering a LV: 47 Machoke. CALM nature. FEMALE gender.
Cross Chop
Rock Smash
Focus Blast
have 51 shiny machamp untouched except for the evo. (cought as machoke) Docile nature, male
Moves:Wake-Up Slap, Cross Chop, Scary Face, Dynamic Punch
PM offers
I have a lvl 32 shiny Machop up for trade, do not PM me UNLESS you offer other shinies or events.
Looking for a Battonage Darkrai
Geneaux~2921 6543 8128Join it!


text:
- Description
Quick Overview
Can't re-touch this! Pros in our L.A. photo studio created this velvety soft powder foundation with Light Filter Technology to instantly blur and diffuse imperfections, Leaving you with a super natural radiance. No lighting crew needed.
- 8-hour, Color-true wear
- Customized, Buildable medium coverage
- Non-drying, Non-caking & non-settling; Oil-free
Smashbox Cosmetics are formulated without Parabens, Sodium Lauryl Sulfate, Phthalates, Fragrance & Talc and are free from animal testing.
How to use product
Choose your finish: - Use the velvety side of the sponge for a medium finish, Or the smooth side for full-on coverage.
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By thechicstyle
Powder foundations are not my thingy, but this one has been best for evening and night parties, people who have dry skin can very well use it for all time purposes and more on the brighter and refreshing look.
Posted on 8/21/2017
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Study Abroad Opportunities
Semester-long, whole-year and summer study abroad programs are available in several different countries. Credit for language requirements may be earned through participation in study abroad programs where language classes are a part of the program. See in particular, the CHINA STUDIES and LATIN AMERICAN STUDIES Programs with Best Semester Programs, the SEMESTER IN SPAIN Program with Trinity Christian College, and CHEZ VOUS Study in France with the Jacques LeFevre Institute.
While many of the programs focus on the study of the target language, some use English as the language of instruction. For more information about the programs listed above, read the detailed descriptions in the Bulletin or e-mail that program's contact person.


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- Age / Gender:
- 29, Male
- Location:
- In front of my computer
- Joined:
- 5/30/04
- All Stats >
I seriously doubt that anyone has ever even looked at my profile.
User Statshot
- Community Stats
Level 15 Blank Slate
Normal Whistle
Ranked as Police Lieutenant
Contact Info / Websites
- Procrastimator
Recent Game Medals
Whyyyyy? 25 Points Kinghood suxx Medal Stats.
SECRET MEDAL 5 Points Unlock this medal to see it's details. Medal Stats.
High Five 10 Points Complete 5 levels Medal Stats.
Dead Giveaway 5 Points Completed the first level Medal Stats.
You Win..? 25 Points That was a weird game. Medal Stats.
Fire and Brimstone 10 Points Burn down all the houses. Medal Stats.
Pull it! 5 Points Pull the switch. Medal Stats.
It's Dangerous to go Alone 5 Points Get the axe. Medal Stats.
A Blocked Path 5 Points Talk to your brother. Medal Stats.
SECRET MEDAL 5 Points Unlock this medal to see it's details. Medal Stats.
Total Medals Earned: 95 (From 28 different games.)
Latest Audio Submissions
|Penny Rapper
|Hip Hop - Modern Song
|I love Midgets
|Miscellaneous Song
|Hunter of Mystical Creatures
|Miscellaneous Song
Latest Favorite Movies
Bitey of Brackenwood Rated 4.3300 Stars Bitey is a nasty piece of work... but he'll get what's coming! Other Final Fantasy A+ Rated 4.2900 Stars Three students work together to save their school in an FF parody. Comedy - Parody Merry Melony ep.1 Rated 4.2600 Stars Melony is caught in a dream that has more to it than first meets eye. Drama God's Message Rated 4.1400 Stars The word of the lord is a light onto my path Comedy - Original


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|43rd week of 2011 patent applcation highlights part 42
|Patent application number
|ELECTROPHOTOGRAPHIC TONER - An electrophotographic toner contains an electron donating color developable agent, an electron accepting color developing agent, and a polyester resin binder which is a polyester resin obtained by polycondensation of a carboxylic acid component and an alcohol component and has a crosslinked structure formed of a crosslinking component including at least either one of a trivalent or higher valent carboxylic acid and a trihydric or higher hydric alcohol, and is decolorized by heating.
|TONER CONTAINING METALLIC FLAKES AND METHOD OF FORMING METALLIC IMAGE - The present invention relates to a porous toner particle with encapsulated metallic flakes. The porous particle containing metallic flakes can be useful for reproduction of a metallic hue upon fusing to a substrate, preferably golden or silvery hue, and for manufacturing of printed circuits, by a printing process, especially electrophotography.
|UPPER LAYER-FORMING COMPOSITION AND RESIST PATTERNING METHOD - An upper-layer film-forming composition includes (A) a resin that is soluble in an alkaline aqueous solution, and includes a fluorine atom, and (B) a solvent component that includes (B1) a solvent having a boiling point at 101.3 kPa of 150° C. or more and a static surface tension of 23.0 mN/m or less, the upper-layer film-forming composition being used to form an upper-layer film on a photoresist film.
|NOVEL DUAL-TONE RESIST FORMULATIONS AND METHODS - Dual tone photoresist formulations comprising a photoacid generator are described and employed in fabrication techniques, including methods of making structures on substrates, and more particularly, methods of making electronic devices (e.g. transistors and the like) on flexible substrates wherein two patterns are formed simultaneously in one layer of photoresist.
|PHOTOSENSITIVE COMPOSITION - Photoresist compositions and methods suitable for depositing a thick photoresist layer in a single coating application are provided. Such photoresist layers are particularly suitable for use in chip scale packaging, for example, in the formation of metal bumps.
|NEAR-INFRARED ABSORPTIVE LAYER-FORMING COMPOSITION AND MULTILAYER FILM - A composition comprising (A) a near-infrared absorbing dye of formula (1), (B) a polymer, and (C) a solvent is used to form a near-infrared absorptive layer. In formula (1), R
|NEAR-INFRARED ABSORPTIVE LAYER-FORMING COMPOSITION AND MULTILAYER FILM - A near-infrared absorptive layer is formed from a composition comprising (A) an acenaphthylene polymer, (B) a near-infrared absorbing dye, and (C) a solvent. When a multilayer film comprising the near-infrared absorptive layer and a photoresist layer is used in optical lithography, the detection accuracy of optical auto-focusing is improved, allowing the optical lithography to produce a definite projection image with an improved contrast and succeeding in forming a better photoresist pattern.
|Method of forming resist pattern and negative tone-development resist composition - A method of forming a resist pattern, including: forming a resist film on a substrate using a resist composition containing a base component (A) which exhibits decreased solubility in an organic solvent under action of an acid and an acid-generator component (B) which generates an acid upon exposure, conducting exposure of the resist film, and patterning the resist film by a negative tone development using a developing solution containing an organic solvent, wherein the base component (A) includes a resin component (A1) containing a structural unit (a0) derived from an acrylate ester containing an acid decomposable group which generates an alcoholic hydroxy group by the action of acid to thereby exhibit increased hydrophilicity.
|RADIATION-SENSITIVE RESIN COMPOSITION AND POLYMER - A radiation-sensitive resin composition includes a resin and a photoacid generator. The resin includes a polymer including a first repeating unit shown by a following formula (1) and an acid-dissociable group-containing repeating unit,
|EXPOSURE APPARATUS AND DEVICE MANUFACTURING METHOD - An exposure apparatus which projects exposure light from a pattern of an illuminated original onto a substrate, comprises a projection system including an optical element and configured to project the exposure light onto the substrate, an enclosure configured to enclose the projection system, and a cleaning mechanism configured to clean the optical element by irradiating the optical element with ultraviolet light under an environment in which oxygen is present within the enclosure, the cleaning mechanism including a light source configured to generate ultraviolet light, a tubular member including an exit window and configured to partially enclose an optical path between the light source and the optical element, and a regulating device configured to regulate an environment of a space inside the tubular member so that a partial pressure of oxygen becomes lower in the space inside the tubular member than in a space which is outside the tubular member.
|METHOD OF CREATING AN EVALUATION MAP, SYSTEM, METHOD OF MANUFACTURING A SEMICONDUCTOR DEVICE AND COMPUTER PROGRAM PRODUCT - According to one embodiment, evaluation map creating method is disclosed. The method determines number (N) of times on changing division starting position of layout for segmenting the layout into areas M to create the map by segmenting the layout into areas m and obtaining evaluation value v corresponding to area m (P
|METHOD OF DETECTING ALIGNMENT MARK AND METHOD OF MANUFACTURING PRINTED CIRCUIT BOARD - When an alignment mark does not exist within an area of an image obtained by a camera, the coordinate of the alignment mark is calculated based on an identification mark existing in the area of the image and a previously stored positional relationship between the alignment mark and the identification mark. A distance by which a long-sized base material is to be moved for causing the alignment mark to be positioned within the imaging area of the camera is calculated based on the calculated coordinate of the alignment mark, and the long-sized base material is moved by the calculated distance.
|METHOD OF ALIGNING PHOTOMASK WITH BASE MATERIAL AND METHOD OF MANUFACTURING PRINTED CIRCUIT BOARD - An exposure system includes an exposure device and an image processing device. The exposure device includes a plurality of cameras. Each of the cameras is configured so as to be selectively set to a full scan mode and a partial scan mode. The camera transmits all of obtained image data in the full scan mode, and extracts part of the obtained image data and transmits the partial image data in the partial scan mode. The image processing device paratactically performs processing using the image data transmitted from the camera and processing using the image data transmitted from the camera.
|PURGE RING WITH SPLIT BAFFLES FOR PHOTONIC THERMAL PROCESSING SYSTEMS - A purge ring for a photonic temperature processing system includes a first layer, a second layer, and a third layer. The first layer, the second layer and the third layer define an inner region. The first layer and the second layer define a first plenum and a first baffle. The first plenum receives a first gas that flows through the first plenum and the first baffle to the inner region. The second layer and the third layer define a second plenum and a second baffle. The second plenum receives a second gas that flows through the second plenum and the second baffle to the inner region, and wherein the second baffle is one of less restrictive and more restrictive than the first baffle.
|NOVEL TARC MATERIAL FOR IMMERSION WATERMARK REDUCTION - Various lithography methods are disclosed herein. In an example, a method includes forming a resist layer over a substrate; forming a coating material layer that includes one of an acid and a chelate compound over the resist layer; and exposing the resist layer and the coating material layer to radiation, wherein during the exposing, the one of the acid and the chelate compound in the coating material layer substantially neutralizes any quencher that diffuses into the coating material layer from the resist layer.
|METHOD OF FORMING RESIST PATTERN AND RESIST COMPOSITION - There are provided a method of forming a resist pattern in which a resist pattern is formed on top of a substrate by using a chemically amplified resist composition and conducting patterning two or more times, the method being capable of reducing the extent of damage, caused by the second patterning, imposed upon the first resist pattern that is formed by the first patterning; as well as a resist composition that is useful for forming the first resist pattern in this method of forming a resist pattern. The method includes forming of a first resist pattern using a resist composition containing a thermal base generator as a chemically amplified resist composition during first patterning, and then conducting a hard bake for baking the first resist pattern under a bake condition such that a base is generated from the thermal base generator, prior to the second patterning.
|USE OF COMPOUNDS CONTAINING HALOGEN AND NITROGEN FOR REDUCING MERCURY EMISSIONS DURING COAL COMBUSTION - Processes and systems are provided for using bromine and/or bromide-containing compounds derived from a nitrogen-containing compound, such as ammonia, sulfamic acid, or one or more salts thereof, to reduce mercury emissions during coal combustion.
|CHARCOAL STARTING SYSTEM - A device, method and system is provided for a quick, convenient, reliable, odorless and non-toxic alternative to using bagged charcoal briquettes. No harmful chemicals are used, ensuring a safe product that also will not diminish or alter the unique charcoal flavor of the foods being cooked over the charcoal briquettes. The tedious process of handling the briquettes is eliminated and no additional kindling for lighting is required. The container is placed onto the barbeque grate or other cooking surface and lights the outer package; the contained components provide sufficient heat and robust flames to ignite the briquettes within the package.
|Thermo-sensing color-changeable candle - A thermo-sensing color-changeable candle includes a candle and thermo-sensing powder combined together. The thermo-sensing powder will change colors and the decorative patterns concealed at the bottom of the candle beforehand will be revealed when the candle is burnt and the temperature of the candle rises.
|METHOD AND APPARATUS FOR APPLYING A MID-IR GRADED-INDEX MICROSTRUCTURE TO AN OPTICAL FIBER TIP TO ACHIEVE ANTI-REFLECTIVE PROPERTIES - A method and apparatus for applying a mid-IR graded microstructure to the end of an As2S3 optical fiber are presented herein. The method and apparatus transfer a microstructure from a negative imprint on a nickel shim to an As2S3 fiber tip with minimal shape distortion and minimal damage-threshold impact resulting in large gains in anti-reflective properties.
|HEAT TREATMENT APPARATUS - A cooling chamber is provided which cools workpieces that have been heated. Cooling units are provided in the cooling chamber, and respectively include: a conveyor which simultaneously and uniserially conveys a plurality of workpieces; a mist cooling device which is disposed so as to surround the conveyance path of the conveyor and which supplies mist-like cooling liquid; and a gas cooling device which is disposed so as to surround the conveyance path of the conveyor and which supplies cooling gas.
|DENTAL MATRIX - A dental matrix comprising a band with an edge for placement toward the gingival margin of a tooth to be restored, and a strip of dental floss attached to and extending along an edge of the band. In the preferred embodiment, buccal and lingual extensions are located beyond a central portion of the band along the edge. The extensions are folded over the strip of dental floss so as to secure the strip of dental floss to the edge.
|Oral Irrigation and/or Brushing Devices and/or Methods - An oral hygiene device having, in one implementation, a unique, peristaltic pump water streaming or jet action; and in another implementation, a multi-headed toothbrush may also be disposed on the end of a handle on which the jet nozzle or nozzles are disposed.
|METHOD FOR FILTERING AND TREATING DENTAL SOLID WASTE - The present application discloses a method of separating solid dental waste from liquid dental waste including capturing the solid dental waste in a permanently filtration unit while the liquid dental waste passes through the filtration unit, transferring the filtration unit to a central storage container; storing the central container until the central container is shipped to a handling facility; and separating the solid dental waste from the filtration unit.
|DENTAL APPLIANCE FOR CONSTRAINING THE TONGUE - Aa dental appliance includes an attachment mechanism suited to attach the appliance on the teeth of a patient's upper jaw. The appliance includes a constraining mechanism linked to the attachment mechanism, where the constraining mechanism is suited to limit the movement of a central zone of the tongue and allow the anterior and lateral edges of the patient's tongue to perform movements necessary for speech and swallowing.
|Natural Tooth Replacement (NTR): An Alternative to Tooth Root Canal (RCT) Therapy and Immediate Root-Form Implant Placement Into Extraction Socket - The present invention relates to the method of replacing an infected, broken, decayed tooth that would otherwise require extraction or root canal therapy with a duplicate ceramic tooth implant uncoated or coated with a series of treatments and manufactured for compatibility by obtaining CAT-SCAN images of the tooth prior to extraction or by utilizing typical 'lost wax' duplicating technology. The invention give the dentist an alternative to performing a root canal procedure (endodontic procedure) on an infected tooth that presents the problematic and in most cases the impossible task of eliminating all the bacteria from the tooth interior and adjacent bone. The bacteriais problematic in that it causes adjacent implants to fail and thus it is presumed to cause other distal chronic inflammatory diseases if these "foci of infections" are not removed.
|Fixture mount-abutments, ball impression screw and fixture mount-abutment drivers - A dental implant fixture mount-abutment, comprising a fixture mount is a 2 to 4 mm coronal extension/metal collar at a coronal end for serving as an impression coping, temporary/permanent abutment and fixture mount, wherein the internal slots in said coronal end for receiving the fixture mount driver, wherein the driven channel in said coronal end for receiving a ball impression screw, wherein said the fixture mount has two small flat facets, located on opposite sides of the fixture mount, extended between said coronal end and circumferential groove, an abutment which is a taper cylinder body having a shoulder and a circumferential curvature, wherein the shoulder on the abutment portion is a part of the abutment, wherein the circumferential curvature is designed to avoid engaging alveolar bone so as to be easily removed later, wherein said the abutment has two large flat facets, located on opposite sides of the abutment, extended between said circumferential groove and shoulder, providing an anti-rotation feature, wherein the ferro is an circumferentially inclined surface to engage the circumferential bevel of a dental implant, increasing the stabilization between DIFMA and dental implant, wherein the 6 wave-shaped protruding features at the coronal portion of the hex-shaped root to provide additional retention, wherein the hex-shaped root of the DIFMA is a male connector for internally connecting to a dental implant. A long DIMFA is an alternative version which contains an extension portion adjacent to the fixture mount; wherein it has a circumferential indentation, two flat surfaces and two semi-circular surfaces. Both two flat surfaces and two semi-circular surfaces are located at opposite sides of the extension portion.
|Soft tissue models and method of making for dental implant applications - The present invention relates to laboratory components that are used in the field of dental prosthesis and for dental implant fabrication and in particular to a spacer means such as a band, ring, or sleeve forming a soft collar which slips over the analog and slides up to the base of the set impression material near the collar of the impression coping as it emerges through the impression forming a uniform blockout around the impression coping which is recording the sub-gingival space over the top of the fixture and having a soft collar surrounding the neck of the analog insulates the top of the fixture analog from the hard stone where it couples with other components. The spacer forming the soft collar is particularly useful for orienting and indexing the modeling of dental implant restorations and to enhance and improve the fabrication process of soft tissue type models for conventional restoration techniques. It also enhances model techniques for digital scanning of cases in the laboratory. The soft collar spacer means forms a uniform blockout around the impression coping which is recording the sub-gingival space over the top of the fixture.
|SURFACE MAPPING AND GENERATING DEVICES AND METHODS FOR SURFACE MAPPING AND SURFACE GENERATION - A surface mapping and generating device which has devices for the process and/or cost optimization, in particular raw material recovery devices, an automatic control of the intensity of a used laser light, devices for carrying out a calibrating procedure by evaluating overlap errors at matching points, devices for archiving especially three-dimensional data of the jaw and/or for modeling the bite position of the upper and the lower jaws, devices for the optimized preparation of at least one dental stump for the production and placement of a dental prosthesis thereon and/or devices for taking into consideration the bite position of the upper and lower jaws. It can be provided that the devices for the process and/or cost optimization are designed to ensure that two half frames showing different positions or views are evaluated, whereby preferably a pulsed laser for exposure purposes is included and/or that an image recording device, in particular a CCD chip, is arranged so as to ensure that lines, taking into account the Scheimpflug angle, are located perpendicular to the direction of travel of a measuring table. Methods according to the present invention use such devices and function correspondingly. In addition, the invention also makes available a patient data archiving system which comprises a chip card and/or decentralized data storage systems especially for dental data.
|ALTERNATIVE ENERGY POWERED ELECTRONIC READER HAVING PRELOADED EDUCATIONAL DATA - A method, system and an apparatus of alternative energy powered electronic reader having preloaded educational data is disclosed. In one embodiment, a method includes converting a solar energy, through a photovoltaic cell, into an electrical current to utilize the solar energy of a sun. In addition, the method includes storing the electrical current in a battery to provide a power source to an electronic reader. The method also includes powering the electronic reader through the battery to provide an electronic content to a user. The method further includes storing the electronic content in a solid state drive in the electronic reader. The electronic content may be an educational lesson.
|SYSTEMS AND METHODS FOR GAZE BASED ATTENTION TRAINING - Gaze based systems and methods are used to monitor the attention of a user. One or more images are displayed to a user on a display. It is determined that an image of the one or more images is being viewed by an eye of the user from one or more measurements received from an eyetracker. One or more modifications to the image are displayed on the display over time so as to maintain the attention of the user. Whether or not the attention of the user is maintained is determined from one or more additional measurements received from the eyetracker after the one or more modifications to the image. In various embodiments, the one or more modifications to the image include moving the image on the display. In various embodiments, the one or more modifications to the image include animating the image on the display.
|SYSTEM FOR ENCOURAGING A USER TO PERFORM SUBSTANTIAL PHYSICAL ACTIVITY - A system for encouraging a user to perform substantial physical activity. The system may include sensors that may be worn by the user while the user is performing a substantial physical activity, such as running or playing basketball. The sensors may detect the magnitude of the physical activity and may transmit data regarding the physical activity to a processing system. The processing system may display a reward to encourage the user for participating in physical activity and the reward provided may be based on the physical activity of the user.
|DEVICE FOR PROMOTING LEARNING OF SKILLS AND CONCEPTS AND METHOD OF USING SAME - A method and apparatus for encouraging a student to continue practicing skills until mastery of the skills is achieved. The method and apparatus present assigned exercises sequentially to a student, and evaluates the student's response. An assignment comprising a finite number of questions, a maximum number of attempts to answer each of the finite number of questions, and a required minimum number of correct answers to the finite number of questions is designed for a user. A learning device presents the user with a question from the finite number of questions that has not been attempted by the user. If the user supplies an incorrect answer to the question, the user chooses to either accept the incorrect answer, or to repeat an attempt to answer the question with new parameters. If the maximum number of attempts to answer the question is not exhausted, the learning device supplies new parameters to the question and presents the question with new parameters to the user, and the learning device reduces the available maximum number attempts to answer the question by one.
|INFORMATION PROCESSING APPARATUS, QUESTIONING TENDENCY SETTING METHOD, AND PROGRAM - Disclosed herein is an information processing apparatus including: a user answer evaluation section configured to determine whether a user answer to a question selected from a plurality of questions is correct or wrong; a user answer analysis block configured to compute at least a user wrong-answer percentage by use of the user answer evaluation result; a questioning condition setting block configured to compute a degree of similarity between the plurality of questions on the basis of the user wrong-answer percentage and, at the same time, compute an evaluation value of each of the plurality of questions by use of the degree of similarity; and a question selection section configured to select a question to be set from the plurality of questions on the basis of the evaluation value and a user correct-answer percentage in one of a predetermined period and a predetermined number of questions.
|Microinjection apparatus and microinjection method - A microinjection apparatus is designed to introduce the transfecting material into the transductant by inserting a minute injection needle (
|METHODS FOR DETECTION OR MEASUREMENT OF VIRUSES - A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.
|SEPARATING TARGET ANALYTES USING ALTERNATING MAGNETIC FIELDS - The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.
|VACCINE AGAINST INFECTIOUS AGENTS HAVING AN INTRACELLULAR PHASE, COMPOSITION FOR THE TREATMENT AND PREVENTION OF HIV INFECTIONS, ANTIBODIES AND METHODS OF DIAGNOSIS - A vaccine for treating and/or preventing infectious diseases where the infectious agent has at least one intracellular phase in the host during its multiplication cycle, is disclosed. The vaccine comprises at least one cryptic epitope of a cellular element that is carried along by an intracellular infectious agent as it leaves the cell, and revealed by said infectious agent. A composition for treating and/or preventing HIV infections, antibodies to a peptide of interest, and a diagnostic method, are also disclosed.
|METHODS FOR THE DIAGNOSIS OF VARICELLA ZOSTER VIRUS INFECTION - The present invention relates to methods and devices for the rapid assessment of saliva for the presence of varicella zoster virus (VZV) particles. The methods and devices permit rapid, simple and cost-effective diagnosis of primary VZV infection.
|SYSTEM AND METHOD FOR MULTI-ANALYTE DETECTION - The present invention provides a system and method for the simultaneous detection of multiple analytes in a sample. The detection system includes a housing that holds a reagent carousel rotatably coupled thereto. Further included in the housing is an incubator carousel rotatably coupled thereto. The housing also includes magnetic material that is associated with the incubation carousel for assisting in separation beads from reagent and wash solution. A robot, associated with the housing is configured to manipulate at least either the reagent carousel or the incubator carousel and transfer materials therebetween. Reaction vessels hold samples and reaction vessels handlers move the reaction vessels. Sample analysis is determined by at least one laser based detector.
|IMAGING TECHNIQUES USING A TRIDENTATE LIGAND - The present invention relates to microscopy and, in particular, Time-resolved Emission Imaging Microscopy (TREM). The Invention relates to the use of a transition metal complex having a tridentate ligand in an imaging technique. The transition metal is preferably platinum.
|Compositions, Methods, and Kits for Amplifying Nucleic Acids - The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.
|FLUORESCENT LABELING OF TRANSFER RNA AND STUDY OF PROTEIN SYNTHESIS - Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system. The disclosed methods are useful in single molecule as well as in ensemble settings.
|Male Reproductive Health Panel and Uses Thereof - A male reproductive health screening panel is disclosed. In some embodiments, the method comprises screening human sperm using a sperm DNA accelerated decondensation (SDAD) Test, and/or a Sperm DNA decondensation (SDD) assay, in combination with an assessment of the sperm DNA fragmentation index (DFI) of the sperm sample of interest. The method is useful in the screening and clinical management for a reproductively challenged human couple, in the assessing the reproductive health of a potential sperm donor, and in selecting an appropriate assisted reproductive technique (ART) for a reproductively challenged couple. These methods provide a decision tree that employs a patient's sperm test results in a SDAD Test, sperm DFI and/or SDD Test. An automated scoring method is disclosed for assessing sperm activity after SDAD and SDD tests. Also disclosed is an automated sperm processing protocol for both the SDD and SDAD tests.
|SUSCEPTIBILITY VARIANTS FOR PERIPHERAL ARTERIAL DISEASE AND ABDOMINAL AORTIC ANEURYSM - The present invention discloses certain genetic variants as susceptibility variants for peripheral arterial disease (PAD) and abdominal aortic aneurysm (AAA). The invention relates to risk management using such variants. The invention further relates to kits for use in risk assessment of PAD and AAA.
|CIS-ACTING DIVERSIFICATION ACTIVATOR AND METHOD FOR SELECTIVE DIVERSIFICATION OF NUCLEIC ACIDS - The invention relates to identification of a cis-acting diversification activator (DIVAC) that is necessary and sufficient for the activation of diversification in transcription units linked thereto. The invention provides a method for diversification of a target nucleic acid comprising introducing a genetic construct comprising the diversification activator into a recipient cell, wherein the diversification activator is linked to the target nucleic acid.
|Modified Proteins and Methods of Making and Using Same - Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
|FLUORESCENT DYES,METHODS OF SYNTHESIS AND APPLICATIONS THEREOF - The present invention provides a category of cyanine dyes having the following general structural Formula I, wherein X is defined as C(CH
|KIT FOR THE OPTIMISATION OF PROTEIN SYNTHESIS/SECRETION - The invention relates to a kit to be used for the identification of a secretion cassette, which in eukaryotic cells gives rise to a higher level of synthesis/secretion of a protein of interest, the coding sequence of which being flanked by the first and the second set of genetic elements, respectively, compared to the other secretion cassettes present in the kit containing the coding sequence at the corresponding position, as well as to a method wherein the kit is used and the use of said kit, whereby the best secretion cassette will be obtained.
|MICROFLUIDIC MULTIPLEXED CELLULAR AND MOLECULAR ANALYSIS DEVICE AND METHOD - A sequential flow analysis tool comprising a microti iridic device having a fluid path defined within a substrate between an input and an output is described. The device includes a capture chamber provided within but offset from the fluid path, the capture chamber extending into the substrate in a direction substantially perpendicular to the fluid path such that operably particles provided within a fluid flowing within the fluid path will preferentially collect within the capture chamber.
|PROCECURE FOR PREPARING A PROCESSED VIRTUAL ANALYSIS IMAGE - This method includes the following steps: carrying out a processing of the specimen so as to make it possible to differentiate the pathological cells from the healthy cells of the specimen; and performing an acquisition of images of the specimen disposed on an analysis plate so as to obtain a plurality of images each representing a zone of the analysis plate, the images placed side by side forming an image of the whole of the specimen so as to create a virtual analysis plate. The method furthermore includes the following step: performing on the virtual analysis plate a processing of the images acquired so as to obtain a virtual restitution of the colors and of the intensity of the colors of the cytoplasm and/or of the nucleus, the colors and the intensity being able to be modified according to the preferences of the person in charge of the analysis.
|ISOLATION OF PROTEIN FACTORS THAT ASSOCIATE DIRECTLY OR INDIRECTLY WITH NUCLEIC ACIDS - Methods for isolating polypeptides and polypeptide complexes that are associated with a target nucleic acid sequence are provided. The methods comprise the steps of obtaining a sample that comprises a target nucleic acid sequence and one or more polypeptides or proteins associated with that target nucleic acid sequence; contacting the sample with at least one oligonucleotide probe that comprises a sequence that is complimentary to and capable of hybridising with at least a portion of the target nucleic acid sequence, wherein the oligonucleotide probe comprises at least one locked nucleic acid (LNA) nucleotide and wherein the oligonucleotide probe further comprises at least one affinity label (e.g. biotin); allowing the at least one oligonucleotide probe and the target nucleic acid sequence to hybridise with each other so as to form a probe-target hybrid; isolating the probe-target hybrid from the sample by immobilizing the probe-target hybrid through a molecule that binds to the at least one affinity label (e.g. using streptavidin-coated magnetic beads); and eluting the one or more polypeptides that are associated with the target nucleic acid sequence. Probes (e.g. with long spacer) for use in the methods of screening are also provided.
|Genetic Markers for Horned and Polled Cattle and Related Methods - Embodiments of the present invention provide methods for improving desirable animal traits including the horned/polled phenotype in bovine animals. Also provided are methods for determining a dairy animal's genotype with respect to multiple markers associated with polled, fitness and/or productivity traits. The invention also provides methods for selecting or allocating animals for predetermined uses such as progeny testing or nucleus herd breeding, for picking potential parent animals for breeding, and for producing improved progeny animals. Also provided are methods for identifying genetic markers associated with polled, fitness and/or productivity traits that are in allelic association with the SNPs disclosed herein.
|MARKER FOR COLON CANCER AND METHOD FOR DETECTING COLON CANCER - In embodiments the expression or methylation of the TBX5 gene is used as a marker for the presence and prognosis of colon cancer. In further embodiments methods for detecting colon cancer are disclosed as are methods for inhibiting the growth of colon cancer cells.
|CORRECTING AN ASSAY IMAGE OF AN ARRAY OF SIGNALS GENERATED FROM A MULTIPLEXED HYBRIDIZATION-MEDIATED ASSAY - Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is complementary to a corresponding wild type allele), with probes bound to encoded particles (e.g., beads) wherein the encoding allows identification of the attached probe. The methods relate to avoiding cross-hybridization by selection of probes and amplicons, as well as separation of reactions of certain probes and amplicons where a homology threshold is exceeded. Methods of correcting a fluorescent image using a background map, where the particles also contain an optical encoding system, are also disclosed.
|METHOD FOR MEASURING DNA METHYLATION - The present invention relates to a method of measuring the content of methylated DNA in a DNA region of interest in a genomic DNA contained in a biological specimen, and so on.
|MARKING - The invention provides a marking system, markers and methods of use of such marking systems and markers which enable unique marking of articles and subsequent detection of that marking. In particular the invention provides a marking system, the marking system comprising a plurality of different DNA fragment types, each of the plurality of different DNA fragment types comprising a plurality of different length DNA fragments and a method in which a sample of the DNA fragment type is taken, amplified and analysed to determine the identity of the marker for an article.
|METHODS AND COMPOSITIONS FOR DIAGNOSING OR MONITORING AUTOIMMUNE AND CHRONIC INFLAMMATORY DISEASES - Methods of diagnosing or monitoring an autoimmune or chronic inflammatory disease, particularly SLE in a patient by detecting the expression level of one or more genes or surrogates derived therefrom in the patient are described. Diagnostic oligonucleotides for diagnosing or monitoring chronic inflammatory disease, particularly SLE infection and kits or systems containing the same are also described.
|METHOD FOR PREDICTING THE ATHLETIC PERFORMANCE POTENTIAL OF A SUBJECT - A method for predicting the athletic performance potential of a subject comprising the step of assaying a biological sample from a subject for a genetic variant in linkage disequilibrium with MSTN-66493737 (T/C) SNP. The invention also provides an assay for determining the athletic performance potential of a subject.
|NON-INVASIVE FETAL RHD GENOTYPING FROM MATERNAL WHOLE BLOOD - The present invention discloses methods of determining the RhD genotype of subject. In particular, the invention provides a non-invasive method of determining fetal RhD genotype from a maternal biological sample containing fetal cells. The invention also provides novel probes and primers useful in the described methods. Kits and mixtures comprising the novel probes and primers are also disclosed.
|MODIFIED NUCLEOTIDES - Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.
|IMPROVED NANOPARTICULATE COMPOSITIONS OF POORLY SOLUBLE COMPOUNDS - The present invention is related to a method for the quantification of one or more target ribonucleic acids in a sample comprising the steps of, (i) providing a sample comprising said one or more target ribonucleic acids, (ii) contacting said sample with a ribonucleic acid specific fluorescence dye under conditions allowing for binding of said dye to the one or more ribonucleic acids in said sample, (iii) measuring fluorescence of said RNA-bound dye in said sample, (iv) correlating said measured fluorescence to the total amount of RNA in the sample, (v) reverse transcribing said one or more ribonucleic acids, thereby creating double-stranded nucleic acids, (vi) amplifying said one or more created double-stranded nucleic acids, wherein one or more fluorescence probes specific for said one or more amplification products are present during and/or after amplification under conditions allowing for binding of said one or more probes to said one or more created double-stranded deoxyribonucleic acids in the sample, (vii) measuring fluorescence of said one or more probes bound to said one or more amplification products during and/or after the amplification reaction and correlating said measured fluorescence to the amount of target RNA sequence in the sample and (viii) normalizing the amount of target RNA sequence in the sample to the total amount of RNA in the sample.
|METHOD FOR PRETREATING SPECIMEN AND METHOD FOR ASSAYING BIOLOGICAL SUBSTANCE - On magnetic particles serving as a first support, an antibody against a nonspecific reaction factor is immobilized. These magnetic particles are mixed with a specimen and suspended therein. After suspending, the suspension is sucked up into a pipette chip and a magnet comes close to the pipette chip. While remotely constraining with the magnet the magnetic particles carrying the nonspecific reaction factor bonded thereto, the residual liquid is discharged into a well. Thus, the removal of a contaminant contained in the specimen is completed. The thus treated specimen discharged into the well is subjected to an immunoassay. The magnetic particles carrying the antibody immobilized thereon are mixed with the treated specimen and suspended therein. The suspension is sucked up into a pipette chip and the magnetic particles carrying an antigen bonded thereto are separated by use of the magnet. The magnetic particles carrying the antigen bonded thereto are washed, mixed with an enzyme-labeling solution, which contains a second support, and suspended therein. After suspending, the magnetic particles being labeled and carrying the antigen bonded thereto are mixed with a substrate solution and subjected to the measurement of emission intensity, etc.
|METHODS FOR PREDICTING SURVIVAL IN CANCER PATIENTS - A method for survival prediction in cancer patients is provided. In one embodiment, the survival prediction is determined by the presence or absence of KRAS gene region deletion and/or loss of Chromosome 12 (Ch. 12) in cancer tumor tissue. In another embodiment, the presence or absence of KRAS gene region deletion and/or loss of Ch. 12 in cancer tumor tissue is used to predict survival in non-small-cell lung cancer (NSCLC) patients.
|Test for the Detection of Bladder Cancer - A diagnostic or prognostic method for detecting malignant and premalignant bladder cancer comprising the identification and quantification of an expression level of identified gene products in the body fluids of a patient and subsequently comparing the expression level of the patient to the expression level found in subjects that do not have bladder cancer.
|APTAMER SANDWICH ASSAYS - The present invention provides methods for identifying the plurality of aptamers that bind to different sites of a target molecule and methods for using the same, for example, in sandwich assays. In particular, the plurality of aptamers binding to different sites of the target molecules is identified from a library of aptamers identified from the same SELEX process.
|METHOD FOR THE QUANTITATIVE DETERMINATION OF THE NUMBER OF COPIES OF A PREDETERMINED SEQUENCE IN A SAMPLE - A method for the quantitative determination of the number of at least one predetermined sequence in a biological sample comprises the steps:
|MOLECULAR ASSAY FOR DIAGNOSIS OF HIV TROPISM - The invention is directed to compositions, methods and kits for HIV subtypes in a test sample, wherein target sequence are amplified. The amplified target sequences are then analyzed by any number of mass spectrometric techniques, which data are queried against a database of base composition signatures of HIV subtypes.
|METHODS FOR ISOLATING A TARGET ANALYTE FROM A HETEROGENEOUS SAMPLE - The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.
|EXTRACTING LOW CONCENTRATIONS OF BACTERIA FROM A SAMPLE - The invention generally relates to conducting an assay on a sample that isolates a bacterium from the sample, in which the assay isolates as low as about 1 CFU/ml of bacteria in the sample.
|ANALYZING BACTERIA WITHOUT CULTURING - The invention generally relates to conducting an assay on a sample that isolates a pathogen from the sample and allows for analysis of the pathogen with minimal (i.e., at most
|DISEASE MARKERS AND USES THEREOF - The present invention encompasses miRNA profiles and type-I IFN/IFNα-induced PD marker profiles in inflammatory or autoimmune disorders, such as myositis. The profiles may also be used in, for example, methods of treating patients, methods of monitoring disease progression of patients, and in diagnosing or providing a prognosis to patients having inflammatory or autoimmune disorders.
|ANTI-HS6ST2 ANTIBODIES AND USES THEREOF - The present invention provides antibodies that bind to HS6ST2 proteins, pharmaceutical compositions comprising the antibodies as active ingredients, methods for diagnosing cancer using the antibodies, HS6ST2 proteins conjugated to cytotoxic agents and pharmaceutical compositions comprising the HS6ST2 proteins as active ingredients.
|Screening and Treatment Methods Using IGS5 Enzymes of the Metalloprotease Family - Polypeptides which are related to the neprilysin enzyme family and have zinc metalloprotease activities and are referred to as IGS5, polynucleotides encoding such polypeptides, vectors containing such polynucleotides, host cells containing such vectors, processes for producing such polypeptides and/or polynucleotides, screening methods for identifying compounds which stimulate or inhibit IGS5 polypeptides and/or polynucleotides, and the use of such polypeptides and/or polynucleotides in therapy of various dysfunctions, disorders or diseases, particularly cardiovascular diseases, metabolic diseases such as diabetes mellitus type II, and neurodegenerative disorders, such as Parkinson's Disease.
|MAST CELL CARBOXYPEPTIDASE AS A MARKER FOR ANAPHYLAXIS AND MASTOCYTOSIS - The present invention relates to use of mast cell carboxypeptidase as a marker for anaphylaxis or mastocytosis in serum, plasma or saliva samples. Detection of elevated mast cell caroboxypeptidase in for example serum samples is proposed for reducing false negatives in relation to diagnosis of anaphylaxis and various categories of mastocytosis by picking up serum tryptase-negative cases.
|COMPOSITIONS - The invention generally relates to compositions that include magnetic particles bound to pathogens in a body fluid.
|COMPOSITIONS AND METHOD FOR ISOLATING MID-LOG PHASE BACTERIA - The invention generally relates to compositions and methods for isolating mid-log phase bacteria. In certain embodiments, the invention provides a magnetic particle conjugated to an antibody that includes an epitope specific for mid-log phase bacteria.
|METHOD FOR EARLY DIAGNOSIS OF ALZHEIMER'S DISEASE USING PHOTOTRANSISTOR - Disclosed is a method for the early diagnosis of Alzheimer's disease. In the method, cells in which a biomarker characteristic of Alzheimer's disease, preferably beta-amyloid, is labeled with magnetic beads are selectively located in the channel region of a phototransistor, and a difference in photocurrent between normal cells and the cells comprising the protein biomarker labeled with magnetic beads is sensed to diagnose Alzheimer's disease at an early stage.
|METHOD FOR DETECTING A PRION INFECTION - The invention relates to an in vitro method for detecting and/or titrating an unconventional transmissible agent (UTA) or a protein of pathological conformation which is a marker for the infectivity of the UTA, in a sample, comprising a step of concentrating the sample before and after digestion with a protease.
|METHOD OF DETERMINING A CONCENTRATION OF ANALYTES OF INTEREST IN A SAMPLE - A method of determining a concentration of analytes of interest in a sample reaction mixture is disclosed. The method can include measuring intensities of the polarized fluorescence of at least one comparative reaction mixture containing different, known amounts of the analytes of interest, and measuring the intensities of the polarized fluorescence of a sample reaction mixture. The method can also include determining the preliminary concentrations of the analytes of interest in the sample reaction mixture by comparing the measured intensities of the sample reaction mixtures at various time points. The margin of error for the preliminary concentration of the analytes of interest in the sample can be determined at the various time points. Finally, the concentration of the analytes of interest in the sample reaction mixture can be determined by comparing the preliminary concentrations and the respective margin of error at the given time points.
|T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS - The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.
|Determination of the amount of hLHB core fragment in a sample from a subject and uses thereof - The present invention provides a method for predicting the likely timing of the onset of menopause for a perimenopausal female subject by determining the amount of hLHβcf in a sample from the subject comprising the steps of: (a) contacting a sample from the subject with an antibody which specifically binds to hLHβcf without substantially cross-reacting with hLH, hLHβ or hLHβcf, under conditions permitting formation of a complex between the antibody and hLHβcf; (b) measuring the amount of complex formed, so as to thereby determine the amount of hLHβcf in the sample; and (c) comprising the amount of hLHβcf in the subject's sample determined in step (b) with either (i) the amount determined for known postmenopausal female subject or (ii) the amount determined for a sample from a known premenopausal female subject, wherein an amount of hLHβcf in the sample similar to the amount of hLHβcf in the known postmenopausal sample indicates temporal proximity to the onset of menopause, and an amount of hLHβcf in the sample similar to the amount of hLHβcf in the known premenopausal sample indicates temporal distance from the onset of menopause for the subject. AS described herein amount is both concentration and pattern of measurement of concentrations in one or more consecutive urine specimens.
|METHODS AND ASSAYS FOR CLASSIFYING FOODSTUFF AND/OR BEVERAGE AND/OR DIET AND/OR NUTRITION REGIMEN AND/OR MEDICAMENT IN VIEW OF AN EFFECT ON THE CARDIOVASCULAR SYSTEM - Subject of the present invention is an in vitro-method for classifying a foodstuff and/or beverage and/or diet and/or nutrition regimen and/or medicament in view of an effect on the cardiovascular system of a subject, comprising determining the relative level of one or more cardiovascular markers.
|CAPILLARY FOR IMMUNOASSAY, AND CAPILLARY IMMUNOASSAY METHOD USING SAME - A capillary for an immunoassay is provided which comprises an insoluble layer of an oxidase formed on an inner wall surface of said capillary, said oxidase being conjugated to a first antibody, and a layer of a hydrophilic polymer formed on said insoluble layer, said hydrophilic polymer layer containing a second antibody conjugated to a peroxidase, wherein said first and second antibodies are capable of binding to the same antigen.
|PREDICTING MORTALITY AND DETECTING SEVERE DISEASE - Measurement of circulating ST2 and/or IL-33 concentrations is useful for the prognostic evaluation of subjects, in particular for the prediction of adverse clinical outcomes, e.g., mortality, and the detection of severe disease.
|OXIDIZABLE SPECIES AS AN INTERNAL REFERENCE IN CONTROL SOLUTIONS FOR BIOSENSORS - Testing of the performance of an electrochemical meter used to measure the presence of an analyte in a biological sample, particularly glucose in whole blood, includes introducing a control solution containing a predetermined amount of the analyte and a predetermined amount of an internal reference compound. The internal reference compound is selected such that it is oxidized at a potential greater than that used to oxidize the analyte, thereby making it possible to distinguish the control solution from a biological sample.
|REAGENT KIT FOR MEASURING FRESHNESS - A reagent kit for measuring freshness, which can be stored at a relatively high temperature with high storage stability, is obtained by lyophilizing reagent solutions each containing a plurality of enzymes and an enzyme protecting agent. The reagent kit includes a first reagent and a second reagent. The first reagent is obtained by drying a frozen product of a first reagent solution containing XOD, NP, an enzyme protecting agent, and a color former under reduced pressure at a temperature not higher than the glass transition temperature (Tg). The second reagent is obtained by drying a frozen product of a second reagent solution containing XOD, NP, AP, an enzyme protecting agent, and a color former under reduced pressure at a temperature not higher than the glass transition temperature (Tg). The enzyme protecting agent is sucrose and/or gelatin, and the color former develops a color by conjugation with a reaction of decomposing Hx into xanthine and uric acid by XOD.
|CONSTRUCTION AND CRYSTALLIZATION OF EXPRESSION SYSTEM FOR RNA POLYMERASE PB1-PB2 PROTEIN DERIVED FROM INFLUENZA VIRUS - The present invention aims to express influenza virus RNA polymerase on a large scale, to crystallize the influenza virus RNA polymerase, and to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs.
|GLYCOPROTEIN PRODUCTION METHOD AND SCREENING METHOD - A method for producing a glycoprotein, which is uniform in terms of functions derived from a sugar chain (e.g., a blood half-life) and physiological activities, i.e., a glycoprotein, which is uniform in terms of the amino acid sequence, the sugar chain structure and the higher-order structure; specifically, a method for producing a glycoprotein, which is uniform in terms of the amino acid sequence, the sugar chain structure, and the higher-order structure, includes the following steps (a) to (c): (a) folding a glycoprotein, which is uniform in terms of the amino acid sequence and the sugar chain structure; (b) fractionating the folded glycoprotein by column chromatography; and (c) collecting a fraction having a specified activity.
|MONITORING A DYNAMIC SYSTEM BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY - The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.
|Isotopically-Labeled Proteome Standards - The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.
|Methods of Quantifying Methotrexate Metabolites - The present invention provides a method for efficiently converting methotrexate polyglutamates to methotrexate in a cellular extract by contacting the cellular extract with gamma glutamyl hydrolase under conditions suitable for efficient conversion of methotrexate polyglutamates to methotrexate.
|GENETIC INCORPORATION OF 3-AMINOTYROSINE INTO REDUCTASES - This invention provides reductase proteins that comprise NH
|REDOX MEDIATORS - The present invention relates to ruthenium and osmium complexes of Formula [M(A)
|COLLECTION DEVICE AND MATERIAL - Swabs, and materials of the present disclosure, and methods of making same, include randomly arranged sea-island bicomponent fibers.
|COLLECTION DEVICE AND MATERIAL - Swabs, and materials of the present disclosure, and methods of making same, include randomly arranged sea-island bicomponent fibers which have randomly splayed terminal ends.
|Phosphate Limited Inducible Promoter and A Bacillus Expression System - An evolvable production strain of
|SHUTTLE VECTOR BASED TRANSFORMATION SYSTEM FOR PYROCOCCUS FURIOSUS - The present invention relates to vectors for transforming archaea and to transformed archaea, and in particular to shuttle vector systems for transformation of members of the genus
|METHODS FOR DETERMINING SODIUM-PROTON-EXCHANGER LIGAND EFFICIENCY - The present invention relates to methods for determining the efficiency of an ion channel ligand comprising (a) contacting a cell expressing the ion channel with (i) plasma of an animal and (ii) the ion channel ligand and (b) determining the effect of the ion channel ligand on the cell.
|CO-CULTURE COMPOSITIONS AND METHODS - Co-culture compositions and methods are described for identifying agents that modulate a cellular phenotype, particularly of neurons or pancreatic beta cells are provided herein, where the methods include co-culturing differentiated cells, wherein at least one of the cell-types are derived from human induced pluripotent stem cells from a subject having or predisposed to a neurodegenerative or metabolic disorder. Co-culture compositions of differentiated cells from two different human subjects are also described.


text:
[hmm, just checked the archives and my reply to Bill wasn't there. Must have sent it to him directly. Here it is again with a minor mod.] Hi Bill, > Mark, > > Why not just use the system described in RFC 1831, ONC RPC? That's a big question that a single email can't answer. The best I can do in a pinch is to reference Roy Fielding's dissertation; http://www.ebuilt.com/fielding/pubs/dissertation/rest_arch_style.htm#sec_5_1_5 But as a quick-and-practical answer, because I want my thermostat to be a first class resource on the Web. This is what the Web was designed to be and do. MB -- Mark Baker, Chief Science Officer, Planetfred, Inc. Ottawa, Ontario, CANADA. firstname.lastname@example.com http://www.markbaker.ca http://www.planetfred.comReceived on Friday, 14 December 2001 01:22:31 UTC
This archive was generated by hypermail 2.3.1 : Tuesday, 6 January 2015 20:38:01 UTC


text:
2007 Lexus RX 350 SUV - Consumer Review
Build Quality
Reliability Value
Build Quality
Reliability Value
14 of 15 people found this review helpful
bought used in Nov2010
By jekcartalk on
Vehicle2007 Lexus RX 350 4dr SUV AWD (3.5L 6cyl 5A)
ReviewWanted to drive the suv for a year before stating my opinion. Traded in a 03MDX (transmission torque converter issue @ 96K miles), and opted for a used 07LexusRX vs new Subaru Outback - $9k cheaper.
Overall I am pleased with my purchase, as the car was a premium package w/Nav etc.
I agree the Nav is a pain to use because of its limited ability to change while on the move (other than voice commands, which is even more frustrating and distraction when it doesn't recognize what you are saying) than being able to input by touch. Only isuue so far... rear wheel bearing hub which was replaced under warranty (70k Miles for drivetrain) which the dealer promptly notified me. So far so good!
Best FeaturesComfortable seats, reasonably quite ride, decent gas mileage 19C/27H - AWD. Handles well in snow. Great travel vehicle for 4 people. Easy Bluetooth set up. 3 power outlets. HID lights. Paint job seems to hold up well against the elements. Power open for rear hatch. Rain sensors operate well. Brakes well. Controls laid out well. Good Customer service- Lexus.
Worst FeaturesStereo system not up to par for luxury car, can not believe it does not have simple input for I phone/MP3 player. Navigation System not great (use Garmin or smart phone). Plus some of the screen functions on Nav system (Info, Audio, etc) could be more intuitive. Air Conditioning warning indicator issue. Fan a little too loud.
Recommend (14) (1)
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text:
Uniform bracket is down to the championship, it is us vs. Iowa, and SOMEHOW we are down 58-42. Lets get these votes going and come out on top like we should!
Link: http://btn.com/2012/03/31/b1g-football-uniform-bracket-title-michigan-vs-iowa/
Uniform bracket is down to the championship, it is us vs. Iowa, and SOMEHOW we are down 58-42. Lets get these votes going and come out on top like we should!
Link: http://btn.com/2012/03/31/b1g-football-uniform-bracket-title-michigan-vs-iowa/
Now. Get to work.
LOL>...i thought we're down....we're leading 60%-40%
really? Wow, must've been only a couple of votes lol
Michigan 60-40....should end up 80-20 at least! After Penn State and Ohio in the BIG there is nobody even close!
Go Blue!
Up to 72% Michigan now
Much easier to pick who to vote for unlike Hagerup v. Falling Raptor
why do peple keep doing brackets like this
And why is Iowa in the finals? Their unis aren't even original! They took them from the Steelers!
So. Our winged helmets first were worn at Princeton. They came to us when Fritz Crisler came to AA.
We hired the man who invented the helmet design. That's a little different than just plain copying another team.
But Crisler brought them to Princeton in the first place. Them when he came here he used them here too. Maize and blue is way better than black and orange anyway.
If we are being technical and smartass-ish and all that shit, Sparty actually donned a "winged helmet" before us but that doesn't diminish the greatness of our unis, does it? Most everyone back in the day had a form of the "winged helmet" but the Michigan unis are great because they haven't been changed in forever (unlike sparty) and weren't copied from a pro team (unlike Iowa).
Edit: And what has been mentioned above, we brought in the man who invented the helmets anyways.
It wasn't so much the wings as the three stripes that was the innovation. Only Crisler's teams had both. And obviously, Michigan was the only team to keep the look going even after plastic helmets were introduced.
anyone claiming that a school has better uni's over michigan or notre dame should be hung, drawn and quartered.
I agree, but I also think PSU and USC should be in the conversation as 'elite' uniforms
I voted and left comments for extra credit.
Michigan has been voted best uniforms in the nation let alone the B10.
75-25. Looks like the good guys will win another one.
We have the best uni's in the country hands down!
Now that a lot more precincts are in, we're up by more than a 70-30 margin.
Iowa ballot stuffing making a run.....!
Yes, that is true.
I think bots are playing for the Haweyes
We need more help here guys! At 10:10 EST, Herky is back in the lead.
The Bots and haters are now coming out in full force. The only way anyone can beat Blue I guess...
Should tell @BTNBrentYarina that his poll just got fixed, since there's been a swing from 70% to 46% in a half an hour.
Done and done.
Let us know if he replies.
Well, he replied, not sure what he intends to do about it. "Ha! That seems to be how it always goes, once message boards get wind of it. Happens in all polls."
But even so, he is bright enough to understand the difference between getting a lot of people to vote (in a poll that only lets you vote once) and a bot manufacturing votes? All of Iowa could have voted and it wouldn't have swung that much that fast.
It won't even let me type in the text part of the comments section.
My assumption is that it's Saturday night and the amount of fucks he gives equals zero.
Come on guys... we can't really lose this one to Iowa. Even if the bots are rooting for them.
Iowa fans start posting in the comments....think one of them had anything to do with it?
How are they leading again?
We need more votes!
We are up against Iowa, sparty, and ohio.
All those Iowa guys not getting laid on a Saturday night are running up the score.
Apparently, even their bots are not getting laid.
47-53 let's get going!
I think Iowa will run out of IP addresses lol
We are at 59% now
60.17 to 39.83
still a bit to close
get those votes in
They have a steelers picture in the post?


text:
Kenny Wayne Shepherd - One Foot On The Path
One Foot On The Path(Difficulty: undetermined)
One foot on the path tabbed by Luca This song is a 12-bar blues based on the riff below. If you play it in the key of G#, the riff will change in C# and D#... of course! The last thing... I used the ^ symbol for legato parts. (Riff in G#) (guitaretab.com)
One Foot On The Path(Difficulty: undetermined)
Date: Wed, 18 Dec 1996 15:22:58 -0500 (EST) On Foot On The Path (Kenny Wayne Shepherd/Mark selby) You say, "Come over baby, come talk to me" Then you drive me so crazy I can't hardly see If this keeps goin' on I might just leave an (guitaretab.com)