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Notes on Looking, July 1, 2010
Hello friends and Fellows,
On July 24th there's a concert I won't be able to attend and so I'm deputizing you to go. (I'll be on summer vacation with David and friends in Parkfield playing with horses and wild turkeys, hiking, revisiting staring white cow and other such exciting things.)
SASSAS at King's Road Park will be featuring the music of Scott Benzel including Music from the Trip (1967) in the style of a Schoenberg-Gershwin tennis match observed in passing by Dr. Oscar Janiger; a live performance of Inversions I – IV (recordings of which premiered in January at Kathryn Andrews' Apartment 2 space); and the world premier performance of Blak Bloc (music for string quartet and guitar feedback).
About Blak Bloc: in the spring of this year, driving on Hwy 58 from McKittrick through cattle country to the California Valley David and I listened to Blak Bloc several times. Benzel creates rich, affecting and generous desolation with these string instruments; sometimes I was thinking I heard sirens, sometimes sweet sounds came through the speakers from a subtly played cello or viola. This lovely and also jarring music fit as well in its native urban habitat as it did out here in the wilds. As we crossed the Carrizo Plain and moved from blasted heath and salt lake to wind-waving grassland (with attendant flying insects) Benzel's music reached a crescendo that then disappeared as sound yet stayed nicely in my mind as music does in the pause after a concert before the applause begins.
Sassas / King's Road Park Pavilion, 1000 N. King's Road, West Hollywood, 90069
Saturday, 11 am. We alight in Die Spinne. Turn left from Wilcox onto Melrose; left on Highland; left on La Brea; right on Venice; left on Fairfax; right on Washington; left on Sentney Ave and park immediately. Gaze across the street and consider that Susanne Vielmetter may be closed – it looks dark in there! Poke the door, it opens and in we go.
Andrea Bowers The Political Landscape occupies two of the galleries as well as some acreage in the office. Two individual projects continue Bowers' politically activist landscape-based work: The United States v Tim DeChristopher and No Olvidado (Not Forgotten) which is about the US / Mexico border and particularly about the undeterminable number (and names) of people who have died in attempts to cross that border.
Shana Lutker. One may read the press release for Shana Lutker's exhibition H.Y.S.T. et al. and approach the work intellectually, but to appreciate the ideas one needs to see the objects. Attend this show. Each of Lutker's sculptures and wall works are worthwhile shapes of odd and the colors she chooses to make them look like flesh tones from a dream. (I'm sure Hugo von Hofsmannsthal when he was writing poetry as "Loris" and when he wrote the Lord Chandos Letter had his rooms fitted out in similar tones.)
After you look at the exhibition allow yourself to wander into the office and spend some time with Lutker's video which has the sculptures animated – some of them preening, some staticly posing for us, one of them with delicate long bent legs placing fawn-like footsteps all around the space. Nice. (Honest it's short, about four minutes. Don't be scared.) Things come together in your mind, you want to go look again at the work.
One of Lutker's inspirations for the show is a young French woman "Augustine" who in 1876 came under the care of J.M. Charcot for what he later termed Hysteria. This Augustine, during her time in the sanitorium, was observed by (and photographed by) Charcot and later shown to Freud. Still later in photographs she was much looked by the Surrealists and by now has been looked at by just about everyone who studies art or psychology. I wonder if in the film the elegant, bent legged sculpture who gets to wander all around, "looking" at the other sculptures is Augustine reincarnate?
You know? I'm forgetting June Gloom at Country Club last week. Crazy of me, it was a great show. I say was because the Fritz Chesnut curated exhibition was only up for the weekend. Particularly exciting for me to see? A painting by Justin Hansch titled Stone (image 21 of 24 on Country Club site); a tapestry sort of piece by Katie Herzog titled (I think) Lithium Forever (image 2 of 24); and an incredibly painstakaingly painted untitled work by Neill Orje (image 17 of 24). Try the Country Club site for these and more images and also click on Try-Har-Der for installation views with humans inhabiting (activating?) the space along with the art. (Also on T-H-D are images of the Catherine Taft curated Bourgeois Problems at Francois Ghebaly.)
Country Club, 805 S. Genesee Ave., LA, 90036
Francois Ghebaly, 510 Bernard St., Chinatown, 90012
Angela Dufresne, Enlightenment Covers is showing at Kinkead Contemporary through July 24. Dufresne makes great paintings. (Gee Geoff, that's helpful! Can you try a little harder?)
These are sort of grand gesture history paintings gone awry. Dufresne does paint really well. I think. Reading her own words in the press release I wonder how much of what I like about her paintings are the "kitsch tricks" and the "pretty and nasty colors" she calculatingly makes use of. I wonder, too whether she's making me aware of the way I'm used by a world that can predict my predeliction for these moves or (heaven forbid) is she… using me? Finally I don't care either way – her paintings are beautiful and if they bug me too, well that's all to the better!
Kinkead Contemporary, 6029 Washington Blvd., Culver City, 90232
At and near 6150 Wilshire John Sonsini, Broad Reminders is closing this weekend – look forward to Caitlin Lonegan and Chris Miles opening on July 10.
Chris Brodahl, The Waiting Room is closing on July 2 at Marc Foxx. Marc and Rodney have a summer group show coming on July 10. Nothing listed yet but what I recall from conversation is that you should hold your breath for new work by Evan Holloway, Jason Meadows, Brian Fahlstrom and Carter Mull. Among others possibly. (On the QT to the nice people at Foxx Gallery – update your artists images. 2008 is a long time ago for Carter Mull's work.) (God I hope you're smiling Marc.)
Uta Barth's show at 1301 PE is also closing this weekend. And I want to take yet another opportunity to thank and congratulate Brian Butler, Isha Welsh and Mieke Marple for getting the website up and running. Beautiful and useful, too! Upcoming at 1301 are three sequential one room one artist exhibitions to see us through the summer: July 10 – July 24 Diana Thater, Laurence Aberhart and Fiona Banner kick things off. See here for more information.
Daniel Weinberg and Peter Mendenhall exhibitions are both up for a while yet.
Marc Foxx, Acme, Weinberg, Mendenhall and 1301 PE
6150 Wilshire Blvd., :LA, 90048
You've got a week to go see Scott Treleaven's Cimitero Drawings at Marc Selwyn, should you be so inclined.
Marc Selwyn Fine Art, 6222 Wilshire Blvd., LA, 90048
Now you either leave your car where it is or drive 2 blocks to Steve Turner Gallery. (After traveling into the future that is. The openings I'm about to talk about won't happen until July 10. Consider this fair warning.) If it's the weekend you can sometimes score parking in the back. However you get there just do it. (Oh god I just quoted Nike. Ick.)
RJ Messineo Attached and Brian Kennon Group Shows.
Hey, I'm stoked! I've been a fan of Kennon's for well, a long time and I'm excited to see his show. IMHO my fandom of Kennon sort of counts as homage to Kennon's own references to fandom in his work. For him it's Cindy Sherman, metal-based music and Richard Hawkins – for me it's… Brian Kennon. (Honest, this is not as creepy as it sounds! I'm kind of a fan-based person. You must know this by now.)
Did you see Messineo's work in Never Very Far Apart at Redcat recently? Her constructed paintings, which may and may not contain paint, often remind one of architecture. Mirrored bathroom medicine chests, windows flowing with screening, stud walls exposed and partially covered with chance shaped plaster board. But. Due to her careful and restrained applications of intent Messineo embeds these simple constructions with evidence of stories. One becomes aware of her restraint when one recognizes an inability to read these personal-seeming stories. It's lovely and satisfying to be trusted with such a lack of direct direction. Beauty is present yet reticent in Messineo's work.
RJ Messineo and Brian Kennon
Steve Turner Contemporary, 6026 Wilshire Blvd., LA, 90036
Moving to Hollywood, Michael Benevento is opening Everlasting Gobstopper on July 8. A pretty impressive summer show! Spartacus Chetwynd, Hélio Oiticica, Lil Picard, Pipilotti Rist, Eva Rothschild, Cindy Sherman, Michael E. Smith. Whoa.
Benevento Los Angeles, 7578 Sunset Blvd., LA, 90046
In 2002 Rothschild was in an exhibition at London's Whitechapel Gallery called Early One Morning, along with Shahin Afrassiabi, Claire Barclay, Jim Lambie and Gary Webb. I've obsessed about this show since I bought the catalog in 2002. Why do I tell you this? Dunno. It's what I do, share my obsessions. Cheers. Early One Morning exhibition Whitechapel link; review in Frieze; review in the Telegraph.
Rothschild showed with Karyn Lovegrove some years ago and Lambie has shown somewhere and another in town. (Will someone please show Afrassiabi, Barclay and Webb in LA or invite them here to talk. I don't think I've seen much beyond a small piece by Webb once a few years ago.)
I'd call ahead for this one since there's no opening date on the website: Ltd. Los Angeles also has a cool summer group show opening soon. Tuesday Afternoon in a Cage will have work by Van Hanos, James Hayward, Scott Reeder, Jan Van Imschoot, Michael Williams. Link to January 2010 Beautiful Decay Magazine photo article about Reeder; Painting seems to be the organizing principle for this exhibition. A fairly disparate group of practitioners, too. I wonder – is the act of painting often about sex with men painters? ("Isn't everything about sex with men?" I heard from the lady in the front row.)
Ltd. LA, 7561 Sunset Blvd., Suite 103, LA, 90046 (Tip: an awning on the street window says, "Antiques & Fine Art. The space is btwn Flawless Auto Group and Langer's Luggage.)
Keeping it within walking distance one may still see The Alchemy of Things Unknown… att Khastoo Gallery. (And if one takes my advice at all one will indeed visit this exhibition.)
Khastoo Gallery, 7556 Sunset Blvd., LA, 90046
Closing this weekend at Eighth Veil is Arik Levy's exhibition Out There Logging.
Eighth Veil, 7174 / 7176 Sunset Blvd., LA, 90046
Overduin and Kite are opening their summer season with an exhibition by Nick Relph on Sunday, July 11 from 6 to 8 pm. For those who like me aren't familiar with Relph's work here's a review of his March-April 2010 show at Gavin Brown's Enterprise in Art in America and here's a link to Gavin Brown site with images. I also found a site that only has to do with Relph because he designed a t-shirt for it (sold out, by the way) The Trilogy Tapes blog and shop. I link you to it because… well would anyone like to purchase a Harry Smith "Transforming milk into milk" limited edition t-shirt? (Sorry – that one's sold out too.)
Overduin and Kite, 6693 Sunset Blvd, LA, 90028
You'll notice that the previous several Hollywood posts are organized not alphabetically nor by date of opening or closing. No, not at all. Geography rules. I have you traveling West to East. Technically, David and I can leave home heading West on Melrose, stop at Kantor (more later), turn right on La Brea (glancing hungrily across the avenue to Pinks where the hundreds are lined up for dogs) turn left on Fountain (here I refer you once again to Elizabeth Taylor's advice on how to "make it" in Hollywood: always take Fountain.) turn right on Curson, park the car at Sunset and walk to the first three spaces. How rockin is that? From O & D we either continue along Sunset to the 101 to Chinatown or go down any number of streets to home at Melrose.
About Kantor Gallery. I have on good authority (hey – I got an email about this. It must be true!) that Kantor is now open on Saturdays. This despite the website's assurances to the contrary. On July 10 from 3 to 6 pm Jennie Ottinger opens in a solo show. Should you have trouble, or desire to confirm openness before you drive and park (easier said than done in this neighborhood!) feel free to email Sasha Bergstrom-Katz at [email protected]
Kantor Gallery, 7025 Melrose Ave., 90038
LAXART is hosting Nine Stories, the UCI Master of Fine Arts exhibition. I've been searchin every which way and am not able to find a website for the event other than the LAXART link above. (Yes indeed, the above link is to the Coasters song that inspired Bas Jan Ader – an earlier UCI Grad – to make some of his magnificently (in)considerable art. This version uploaded complete with scratchy record sounds. Check it out: the first of the Highest Rated Comments just breaks my heart. "I may be 15 but hey this is a great song! I would have loved 2 grow up in the 50's with my pa!" Don't even try not to tear up.) I'll link to as many artist websites as I can find.
Nina T. Becker;
April Friges;
Simon Hughes;
Alison O'Daniel;
Andrew Printer;
Shane Quentin;
Betsy Seder who you may also recall from an exhibition at Las Cienegas Projects with Ray Barrie and David Lamelas;
Stephen Walters;
Jenny Yurshansky, you may recall Yurshansky's A Chance Operation show at Workspace.
Nine Stories at LAXART; Saturday, July 3 6 to 9 pm
LAXART, 2640 S. La Cienega Blvd., LA, 90034
Thanks to the completeness of my friends at Actual Size website I actually can list the artists and galleries participating in Cal Arts Box Scheme Master of Fine Arts exhibition:
Actual Size, 741 New High Street, Chinatown, 90012, 213-290-5458 for information
Cottage Home, 410 Cottage Home Street, Chinatown, 90012, 310-428-2964 for information
Dan Graham, 506 Bernard St., Chinatown, 90012
Charlie James, 975 Chung King Road, Chinatown, 90012, 213-687-0844 for information
The Company, 946 Yale Street, Chinatown, 90012, 213-221-7082 for information
Cottage Home Cal Arts Grad Students
Um, here's the hard part. The artists in the combined Art and Photography / Media Grad Show are:
Carrie Rebecca Armellino;
M. Blake Besharian;
Erich Bollmann;
Bjarki Bragaso;
Deborah Christ;
Kelly Cline;
Diana DeAugustine;
Travis Diehl;
Patricia Fernandez;
Nicolas Grenier;
Margaret Haines I can also suggest you visit this pdf document of Haines's work. If this fails (it's a slow download) then on her main page click on Arielle de Pinto 2010 Collection. Cool stuff;
Greg J. Hayes;
Vivian Joyner;
Steve Kado seems to have a lot going on: Kado interview on Youtube and I suspect the Blankett and Veneer Magazine have something to do with Kado. In any case the Veneer site is more fun than Disneyland!
Karolina Karlic,
Ari Kletzky,
Zach Kleyn and (only because this caught my eye and my sense of humor) Greenspan Trance, 2008, an audio song using samples of Alan Greenspan's testimony to Congress,
Keith Rocka Knittel and here's a scoop I got by relentlessly clicking – at his Cottage Home installation Knittel will show his installation We're All Haunted by Michael Asher's Ghosts;
Rena Kosnett, I'm going out on a limb and linking you to some other Kosnett treat-related-sites: rabbi cosmic and Kosnett's Flickr site;
Marilyn Lowey;
Rachel McRae, McRae also has one of those "Under Construction" sites. Hey Rachel, don't feel pressured – I've been watching Amanda Ross-Ho's site for the better part of a year and still it's Coming Soon;
Sage Paisner, here's a nice about page for Paisner,
Carl Pomposelli,
Rasmus Røhling,
Alise Spinella and also a link to Chicago based Harold Arts again because I make the choices on Notes and this residency program sounds completely interesting and worth attention;
Orlando Tirado Amador, and also on a mysterious alternate Cal Arts Box Scheme site. His name doesn't appear on the main site. Oh, I get it. Joker or dreamer? Amador is listed on the main site as Fire Semen. You go guy;
Cody Trept;
Arnoldo Varga;
Silke Zeidler.
Each list one makes is fraught with danger. Have I left someone out? Or unfairly represented someone? I hope not on both counts. Please shake a few hands if you visit the openings and say a huge and heartfelt congratulations to the various and triumphant (and probably broke) graduates. Yay! Yay!
A couple of things before I leave.
Blum and Poe have absolutely a lot going on this July 3. From when to when? Probably aim for 6 pm tp 9 pm and you won't go wrong.
B & P, 2727 S, La Cienega Blvd., LA 90034
Las Cienegas Projects has one show closing this weekend (click to find out who, it's getting late!) and then opening on July 10 Aaron Wrinkle (he of Dan Graham space) will dismantle the space of the gallery and remantle it with his life. It's all a bit fuzzy to me just now but I trust Wrinkle to make it great. Come to LCP many times through July and August!!
LCP, 2045 S. La Cienega Blvd., LA, 90034
Pepin Moore, a new face in town occupying an estimable space. Not going to tell you, you figure it out. (Oh all right. China Art Objects are moving to the former MC space on Comey in Culver City.) Drew Heitzler has curated Animal Style: The New Warrior for the inaugural show with Justin Beal, Scott Benzel, Gil Blank, Jedidiah Caesar, Matthew Chambers, Fritz Chesnut, Krysten Cunningham, Mark Hagen, Emelie Halpern, Lucy Raven. Does this sound good to you? Some that you recognize, some that you hardly ever heard of? (I like music links. You may not.) Just you wait. Parties are way better in Chinatown. Art is too. (Of course I say that to all the neighborhoods.)
Pepin Moore, 933 Chung King Road, Chinatown, 90012
Redcat on July 9 is opening Chen Chieh-Jen: Empire's Borders II – Western Enterprises, Inc. Films. More later, I'm sure. Check out the theater schedule, too. Mike Kelley / Michael E. Smith A Voyage of Growth and Discovery is screening on July 12 and the New Original Works Festival opens on July 22.
Redcat, 631 W. 2nd St., LA, 90012
Bye! Thanks for readingg
Geoff Tuck


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Pros: Inexpensive, easy to install and operate. Can save me from many delays.
Cons: Reports only what information it is provided. Not it's fault.
I had my TomTom device for a while, and was getting more concerned with traffic jams and incidents, so I decided to buy this sensor. Note: The traffic sensor reports freeway information only!
Installation and setup:
The sensor replaces the vehicle power cord. As such, one end plugs into a typical vehicle power/cigarette lighter socket. The other end plugs into the USB jack on the bottom of the unit.
Once powered up, the GPS will indicate the traffic sensor is plugged in, and a vertical information bar will appear on the right side of the GPS unit. The unit will begin scanning for a radio frequency that carries traffic information. By accessing your preferences on your GPS unit, you can also tune directly to a radio channel you know has the RDS/TMC data. Once the unit has tuned and acquired a signal, a solid green indicator will display at the top of the traffic info bar.
First, enter and accept your destination. After that, you will see the traffic information bar change. At the bottom, you will see an arrow symbol. This is you at your current position. At the top there is a "checkered flag" icon, which indicates your destination. It, on your route, there is a traffic incident, there will be an icon somewhere in the line between the two points. This icon will also be labeled indicating the distance to the incident. Your gps display will tell you the delay time, and suggest an alternate route, if there is one that will get you to your destination faster.
You also, can tap the traffic display and get a menu. The items it contains diffter with models of gps receiver; but they typically can give you a list of traffic incidents in your area, traffic on your route, and other items.
This sensor only reports traffic conditions on interstates, and other major freeways in an area. Also, only in areas with the RDS/TMC traffic data broadcasts, can you get information.
The completeness, reaction time and coverage ares of traffic information is dependent on how each area's Intelligent Transportation Systems are configured.
What one user in one area may experience can differ from a user in another.
My experience:
I, personally, have found the information generally quite accurate. I live in Columbus, Ohio, and the ITS infrastructure is fairly complete. Additionally, there are several RDS/TMC stations in place. I have been redirected a few times, and have been pleased with the information provided. On a recent trip, we have received traffic data, although, no incidents were reported or encountered.

subset: commoncrawl


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As new "Galaxy Note III" images arrive, an alternate explanation surfaces
Yesterday, we brought you some images of a device purporting to be the in-development Galaxy Note III, with what looked like a big six-inch screen. Today, that's joined by a new picture, apparently showing the Note III next to a couple other phones. No soon has it hit the scene, though, that we're hearing claims that it's a fake (or at least being misrepresented) and what we're really looking at is the Galaxy Mega 6.3. Does that theory hold up to scrutiny?
GSM Arena prepared the lovely analysis of the new image above, using known measurements of that Nokia handset to identify the screen sizes of those other models. With a 6.3-inch display, rather than the 5.99 or just straight-up 6 inches we heard rumored, couldn't this actually be the Mega 6.3?
We're not quite convinced. The handset's size, earpiece, and camera placement sure align nicely enough with the Mega 6.3, but what about the phone's body? The Mega 6.3 has much more gradual curves than what we see here, which are rounded-off hard right angles.
Maybe it's the Mega 6.3 with an alternative body design – a consequence of Samsung's development process. We can't say, but while this might not be a Note III, it also isn't a production Galaxy Mega 6.3, either.
Separately, TechTastic has its own doubts about those earlier "Note III" images, upon spotting one showing the phone with an S Memo directory, rather than the S Note we'd expect to find.
Source: GSM Arena, GforGames, TechTastic
Via: @evleaks


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LG Mach
Info Photos Reviews News Forum 1
LG Optimus G and LG Mach Arrive November 11 on Sprint
Sprint today revealed launch details about the LG Optimus G and the LG Mach. The Optimus G can be preordered starting today for $199.99 with a new two-year contract. The Optimus G goes on sale November 11, but Sprint said it will try to ship preorders before then. The LG Mach will also be available online and in stores beginning November 11. It will cost $99.99 with a new two-year contract.


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If you think John Herdman is going to sit back, relax with his feet up, and watch the upcoming FIFA U-20 Women's World Cup from the comfort of his living room, think again.
The 16-nation competition runs from Aug. 5-24 with games in Toronto, Montreal, Edmonton and Moncton, and will feature a promising Canadian side. Among Canada's top prospects are Sura Yekka, Kadeisha Buchanan, Rebecca Quinn and Jessie Fleming, who have all played for the senior team and could be named to the squad for next summer's FIFA World Cup to be held in Canada.
For a limited time get Sportsnet Magazine's digital edition free for 60 days. Visit Appstore/RogersMagazines to see what you're missing out on.
Naturally, as coach of the Canadian senior team, Herdman is particularly interested in how these players perform in this tournament. But he also wears the hat of Canada Soccer Women's EXCEL director—a program that "unites and strengthens the ways (Canadian soccer) identifies and develops the most promising young female athletes and helps drive women's soccer forward."
As EXCEL director, Herdman oversees Canada's U-14, U-17, U-18 and U-20 programs, making sure they are all aligned and working in unison towards the same goal: developing more players the calibre of Christine Sinclair, and more often.
Herdman took time out of his busy schedule to talk to Sportsnet about the EXCEL program, the U-20 World Cup, and the importance of the tournament for the senior team ahead of next year's FIFA Women's World Cup.
Sportsnet: The average person might not see or understand how the FIFA U-20 Women's World Cup is actually vital to your preparations for next year's World Cup, and the future of the women's team beyond that. Why is it so important?
John Herdman: It's a great opportunity for these younger players to experience a home World Cup. Over the last year and a half, you've seen how we've integrated quite a lot of these under-20 players into the senior team and given them a lot of game time. That signals they could be a big part of our attempt at the World Cup in 2015. So to play at a FIFA event here in Canada, you can't get a better dress rehearsal for the younger players. That experience, more than anything, is what they lack. This is perfect for us.
SN: What's your evaluation of Andrew Olivieri since he took over as coach of the Canadian U-20 team in 2012?
Herdman: In terms of the under-20s, Andrew has done a very good job of aligning the approach and the system of producing women's national team players. He's done a fantastic job over the last two years of putting that mantra as the priority and bringing into the youth system the same philosophies, paying styles, and playing models that we operate with at the senior team level.
SN: Why is that so important?
Herdman: With the launch of the EXCEL program this year, we shifted our system to make it a top-to-bottom system—full alignment, from the under-14 team through to seniors. We've identified a curriculum, we've put performance standards in place, and each coach in the system, they're their own leader.
Andrew's role is the U-20 EXCEL director, and Beverly (Priestman) is the U-17 EXCEL director. That means they have more than the responsibility of just coaching the team—they have to implement the strategies that lead to this consistent alignment. Part of Andrew's measurement of his success is the style of the team and the amount of players who move on to the senior team—not many how World Cup games he wins.
The challenge that we have here in this cycle of our national EXCEL system is that you have a home U-20 World Cup, and that's quite special. But while we've asked Andrew to make sure he's progressing the team in line with the future of where we want to see the game growing in Canada, at the same time he has to pick a team that can be effective at the World Cup, and give this country something to celebrate.
SN: What's the working relationship like between you and Andrew?
Herdman: It's fantastic. He was my assistant with the senior team through the 2012 Olympics. I oversee the EXCEL program and I provide Andre and Bev support to make sure we're on the same page, and that they're given clear objectives by myself. It's this sort of cohesion that's ensured there is a shared vision for the future and for a future that, whether I'm here or Andrew is here or Bev is here—whoever is here—we've left a legacy of a developmental system that can put Canada on the podium.
Andrew and Bev do have some autonomy, but they do report to me. The goal is to create more Sinclairs more often.
SN: What's your role during the upcoming FIFA U-20 Women's World Cup?
Herdman: That's when I put my other hat on as the Women's EXCEL Program director. I'll be with the team, providing mentorship, guidance and support to the players, and observing and assessing the performance from Andrew. I'll also be working with the players we've identified as having a god chance of getting called up for next summer's World Cup.
What's important in the EXCEL program is that we've started to look at FIFA youth tournaments in a different light. We've identified the youth tournaments as opportunities to learn how to win tournaments. Rather than looking at U-20 as a pinnacle for a player, it's more about achieving certain standards within our system. The tournament becomes about learning certain processes for the players: how to deal with the experience of being in the FUFA bubble, the travel, the recovery time between games, the nutrition cycle, the psychological aspects.
It's a great opportunity to win a World Cup, and if that happens it happens. But for me, it's about players such as Sura Yekka and Kedeisha Buchanan and others who will be with the senior team next summer knowing what to expect. There's a real learning opportunity here, and that's where I see the biggest benefits for the players.
SN: Generally speaking, how competitive is the U-20 women's game?
Herdman: It's ultra-competitive. If you look at the last U-17 world cup, you had Venezuela go to the semifinals—that's not something you'd see at senior level, but it gives you an idea of what is happening at youth level. You'll see teams like South Korea win the event. You'll see Spain win the European championship. You actually start to realize that a lot of countries have put a lot of developmental resources into their system, and their cycles of players in six to eight years would suggest the gap will get smaller and smaller at senior level.
SN: What do you make of Canada's opening round group (Finland, Ghana and North Korea) at this U-20 World Cup?
Herdman: If you look at the group, on paper it looks good. Finland has a small population, and Ghana has a small amount of female players playing the game. So when you consider the national team setup of these countries, you say to yourself Canada has a great chance of being successful.
But the reality is their youth programs have been centralized and you never know what they've done in their system to get the team ready. On paper, it looks like quite an easy group, but the reality is that each of these countries might have a cycle of really good players and their youth system might be at its peak. You just don't know.
FIFA U-20 Women's World Cup: The 2014 FIFA U-20 Women's World Cup runs from August 5–24 in four cities across Canada. Watch all 32 games live on CBC and Sportsnet. || Match schedule || Standings || Tournament preview || Spotlight on Canada's terrific trio || Sportsnet Q&A: John Herdman


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(The Zenwalker's Guide)
Written by the ZenDoc Team.
Copyright © 2005-2008 - Zenwalk Linux
Last revised: 18 April 2008
To master any Linux distribution, documentation is necessary, and Zenwalk is no exception. This manual is heavily based upon the excellent Slackbook. These pages contain information about:
Good luck, and happy Zenwalking!


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Weldon Kees
Weldon Kees (1914 - 1955 / Nebraska / United States)
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In the broken light, in owl weather,
Webs on the lawn where the leaves end,
I took the thin moon and the sky for cover
To pick the cat's brains and descend
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text: On Tue, 15 Jun 1999, Werner Almesberger wrote:> > With RAID 1, lilo can install multiple copies of LILO, one on each disc,> > each one told about the blocks which exist on its own disc.> > Well, technically it could, because it happens to know where the data> goes. However, this would involve allocating space in the RAID, via> the file system, and then filling it via direct writes to the underlying> devices. Pretty horrible, if you ask me.> > No, as far as LILO is concerned, I consider this a case of policy at a> higher level. LILO just writes a single boot loader in a well-defined,> clean way. If there's replication in the RAID, you install multiple> boot loaders, and you tell LILO explicitly where they go.When this was discussed a while ago (maybe on another list?), Isuggested a reserved space at the beginning of each RAID partition.That seemed to encompass the least amount of work on the part ofeverybody.When installing a kernel, a special ioctl (before any writes occur)would tell the RAID software that the file being written should bestored in the special reserved area. When the file is actually written,whether it be RAID0/1/5/whatever, the file output is multiplexed to areserved space at the beginning of each partition in the md set.At that point, LILO need only be extended to output to multiple MBRs/bootsectors, with each MBR pointing to the md reserved space on thatparticular drive. It might need to pass a magic cookie or two to thekernel to tell md it is booting off a secondary boot sector. Jeff-To unsubscribe from this list: send the line "unsubscribe linux-kernel" inthe body of a message to firstname.lastname@example.com read the FAQ at http://www.tux.org/lkml/


text:
Reiki healing is a way to channel your own energy to heal your body. This method uses guided meditation and guided imagery to help you feel more relaxed, balanced, centered, peaceful, and happy.
This audiobook will help you:
This audiobook also includes:
The benefits of meditation, particularly reiki healing, include lowered blood pressure, lowered stress hormones, reduced stress, and an improved mood. This audiobook takes you through a basic reiki therapy session, which will help you feel more relaxed and at peace.
©2015 Lexie Hay (P)2015 Lexie Hay
"Quality very bad"
Went to bed really excited coz of this book iv had a few different ones and they are just amazing so i started listening and the background music kept stopping after 3 seconds then repeating... over and over the whole time with a bird yapping on I nearly jumped out of bed like the Cadburys Parrot going bersurk! !!!!
I could not listen or relax coz of this annoying repeated noise... its through the whole book.... iam sure he is Amazing the music has totally ruined this probably 10/10 book....
"Felt a Difference after One Session"
I have done a few Reiki healing sessions before, so I am a little familiar with the process. This session wasn't that long, only around 15-20 minutes, with the rest being very soothing music (I actually almost fell asleep listening to the soundtrack after the meditation ended). It was an overall very relaxing experience and I will be listening to it again several times.
I found the melodies and nature sounds quite calming and they really helped me get in the mood for meditation. It can be difficult for me to relax, even in the morning before the day has even started. However, I was able to get ready for the meditative state while listening to the introduction. The meditation allows you to take your time, and never felt rushed in any way. Quite the opposite actually - I felt my mind saying "hurry up", and realized I was trying to rush through my own meditative state like a box on a checklist. I need to work on slowing down my mind. Not everything in life needs to happen as quickly as possible, and meditating like this helps me practice this notion.
"Where is the content?"
Was I missing something? It was just music, nice music, but still. Perhaps my download was messed up, but it was just all music!
"The chirping bird killed it"
Perhaps this shows how distracted, annoyed, and cranky my brain can be, and how much I need meditation, but the chirping bird every other second was beyond irritating. I couldn't focus past the bird!!!! The narrators voice was soothing but please choose different music.
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text:
On April 12, 2005 Jeff Simpson, one of three men who have accused Mayor Ed Murray of sexually abusing them when they were teenagers, wrote a light hearted letter to Murray wishing him well.
Simpson even enclosed a photo of him and his wife. Bob Sulkin, Murray's defense attorney provided the letter to KING 5. Sulkin said it's an important piece of evidence.
"The…hand written letter puts to rest any claim that Mr. Simpson was mistreated by the Mayor…This letter shows that Mr. Simpson's claims are…false. Hopefully, this smear campaign will end," said Sulkin.
Simpson started the letter with an upbeat salutation.
"Hey Ed, I hope this letter finds you in good spirits."
Simpson, who is now 49 years old, continued by writing he had seen Murray on television answering questions from reporters. At the time Murray was a Senator in the Washington state legislature.
"You looked very good on TV yesterday. It was good to see you and I must say you looked as though you were in your element. I swelled with a sense of pride watching you fielding questions from the press and watching your colleagues thanking you for all the work you had done. Anyway, good job!"
Simpson signed off by saying:
"God bless you Ed. Love ya lots, Jeff."
Simpson wrote this letter 18 months before he unsuccessfully attempted to file a lawsuit against Murray for alleged sexual abuse. Simpson accused Murray of beginning the abuse in the 1980s when he was 13 years old and later that Murray paid him for sex.
"I was writing him to thank him and I had seen him on TV. (To understand why I wrote it) you would have to know me," Simpson said. "I'm an orphan…I have never had any parents. Ed filled those shoes for me."
In the end Simpson's attorney said he thought too much time had lapsed to continue with the suit against Murray in 2007.
"When I was in drug (recovery) my sponsor encouraged me to come forward and pursue the lawsuit (in 2007)," said Simpson by telephone on Thursday. "When I wrote the letter in 2005 I hadn't accepted what had happened yet."
Simpson said his relationship with Murray has been "confusing." Simpson's parents left him in state care when he was 3 years old. He met Murray at the age of six at the Parry Center for Children in Portland where he said Murray, in his 20s at the time, was a volunteer. Simpson said the abuse began when he was 13 and continued when Murray took Simpson in to live with him.
"I'm grateful that Ed took the time to pay attention to me and for the love that he had for me," said Simpson. "He has done some terrible things to me, but Ed is the only person I know as a parent and father. Do I hate him? Absolutely not. I understand that he is sick…Ed continues to victimize me by calling me a con artist and a liar."
More details came to light Thursday about Simpson's criminal past that Simpson said he's "ashamed of." He has convictions that include false reporting to police, robbery, attempted burglary, forgery, prostitution, and attempted kidnapping.
"Mr. Simpson's long criminal record establishes that he will engage in deceitful conduct to obtain money and hurt others. This record also establishes that he has made false claims against others who tried to help him. In fact, he has used eight different aliases. In short, one cannot even trust him when he gives his name," said Sulkin.
Attorneys representing the plaintiff in the lawsuit filed on April 6 criticized Murray's camp for statements made about the alleged victims.
"I prosecuted serial pedophiles for years as a senior deputy prosecutor in King County. Our client and the other victims who have come forward have independently described strikingly similar accounts of Mr. Murray's methodology of sexually abusing vulnerable young men," said attorney Julie Kays. "Given these striking similarities….Mr. Murray's decision to attack our client, and the other victims, smells like nothing more than desperation."
Simpson said he has been drug-free for years and no longer engages in criminal activity. He said he had not talked about the abuse for years but is speaking out now after being contacted by the Seattle Times for an article that revealed another man, known only as D.H., filed a lawsuit with similar allegations against the mayor.


text:
The 70-kDa heat shock proteins (Hsp70) are chaperones with central roles in processes that involve polypeptide remodeling events. Hsp70 proteins consist of two major functional domains: an N-terminal nucleotide binding domain (NBD) with ATPase activity, and a C-terminal substrate binding domain (SBD). We present the first crystal structures of four human Hsp70 isoforms, those of the NBDs of HSPA1L, HSPA2, HSPA5 and HSPA6. As previously with Hsp70 family members, all four proteins crystallized in a closed cleft conformation, although a slight cleft opening through rotation of subdomain IIB was observed for the HSPA5-ADP complex. The structures presented here support the view that the NBDs of human Hsp70 function by conserved mechanisms and contribute little to isoform specificity, which instead is brought about by the SBDs and by accessory proteins.
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Citation: Wisniewska M, Karlberg T, Lehtiö L, Johansson I, Kotenyova T, Moche M, et al. (2010) Crystal Structures of the ATPase Domains of Four Human Hsp70 Isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78. PLoS ONE 5(1): e8625. https://doi.org/10.1371/journal.pone.0008625
Editor: Nick Gay, University of Cambridge, United Kingdom
Received: November 9, 2009; Accepted: December 14, 2009; Published: January 11, 2010
Copyright: © 2010 Wisniewska et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The Structural Genomics Consortium is a registered charity (number 1097737) that receives funds from the Canadian Institutes for Health Research, the Canadian Foundation for Innovation, Genome Canada through the Ontario Genomics Institute, GlaxoSmithKline, Karolinska Institutet, the Knut and Alice Wallenberg Foundation, the Ontario Innovation Trust, the Ontario Ministry for Research and Innovation, Merck & Co., Inc., the Novartis Research Foundation, the Swedish Agency for Innovation Systems, the Swedish Foundation for Strategic Research and the Wellcome Trust. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The heat shock-70 proteins (Hsp70s) are chaperones involved in crucial cellular functions in all kingdoms of life. Hsp70-family function, structure, and mechanisms have been studied to great detail using cognate HSPA8/Hsc70 [1], stress-induced HSPA1/Hsp70-1/Hsp72 [2], [3] the ER-resident HSPA5/Bip [4], and the bacterial ortholog, DnaK [5]-[7]. Hsp70 proteins bind and release client polypeptides in a cycle that is coupled to an ATPase activity [8]. The overall domain structure is conserved: The N-terminal nucleotide binding domain (NBD) with ATPase activity is joined by a flexible linker to the C-terminal peptide substrate binding domain (SBD). Alternation between the ATP state with low affinity and high exchange rates for clients and the ADP state with high affinity and low client exchange rates is tightly regulated by several classes of associated proteins, or cochaperones [9]. Allosteric communication with the SBD and interactions with cochaperones and nucleotide exchange factors all critically depend on the conformation of the NBD. Therefore, understanding the conformational changes in the NBDs of Hsp70 proteins is key to understanding how the ATPase motor drives the client binding and release cycle of the Hsp70 machine.
The human genome contains over 40 hsp70 sequences. Many of them are pseudogenes, but at least eleven distinct genes located on several chromosomes are translated into protein [9]–[11]. These Hsp70 isoforms differ from each other by amino acid sequence, expression levels, subcellular localization, and induction in response to different cues. Both constitutively expressed and stress-induced Hsp70 proteins have been identified [12]. Constitutively expressed Hsp70 chaperones have housekeeping functions such as the folding of nascent polypeptides, protein translocation between cellular compartments, degradation of unstable and misfolded proteins, and regulation of assembly and disassembly of protein complexes. Other Hsp70 members can be induced by various cellular stresses, such as heat stress, heavy metals, radiation, ischemia, nitric oxide radicals, or other stimuli that activate stress transcription factors. Stress induced Hsp70s prevent accumulation of stress denatured proteins.
Several of the human cytosolic Hsp70 isoforms have yet to be extensively characterized. Heat shock 70-like protein-1 (HSPA1L, also called Hsp70-hom or Hsp70t) is a constitutively expressed, non-inducible cytosolic protein with high abundance in testis [13], [14]. Polymorphisms in the HSPA1L gene, predominantly in the region coding for the client binding domain, have been linked to disease [15], [16]. HSPA2/Hsp70-2 is a constitutively expressed protein with high levels in testis [17], [18]. HSPA2 is essential for maturation of male gametocytes [19] and is linked to male infertility [20], [21]. While it is undetectable in many tissues HSPA2 is involved in cancer cell survival [22]. The HSPA6/Hsp70B' gene is strictly inducible with no detectable basal expression [23], [24]. The HSPA6 protein is nuclear and cytosolic. HSPA6 induction is a sensitive biomarker of cellular stress; the protein appears transiently in response to heat stress whereas HSPA1A/Hsp72 levels persist for days [25].
The major Hsp70 protein of the endoplasmic reticulum is HSPA5, originally called immunoglobulin heavy chain binding protein (BiP), or glucose regulated protein-78 (GRP78) [26], [27]. HSPA5 has crucial roles in the assembly of ER proteins and protein complexes and the unfolded protein response [28]. Both HSPA5 and its interaction partners are linked to a number of diseases including infectious diseases, inherited diseases, and several types of cancer [29]–[31].
Despite the large number of NBD crystal structures determined under different nucleotide conditions, the conformational changes that ultimately drive the Hsp70 chaperone machine have never been visualized by X-ray crystallography of NBDs alone. Instead, the NBDs all crystallized in a closed conformation that is interpreted as resembling the ATP-bound state. Only crystal complexes of NBDs and nucleotide exchange factors have yielded structures with a partially opened nucleotide binding cleft [32]–[36]. Also, the small cleft between subdomains IA and IB that is expected to adopt different opening states in allosteric regulation of the SBD [7] looks similar in all NBD crystal structures. We used X-ray crystallography to address the question whether previously less well characterized human Hsp70 isoforms might adopt different conformations in the absence of binding partners. We determined the structures of the ATPase domains of HSPA1L, HSPA2, HSPA5, and HSPA6. Despite different nucleotides and divalent cations present during protein purification and crystallization these structures are highly similar to the crystal structure of HSPA1A and other previously determined Hsp70 NBDs. HSPA5 crystallized with ADP and calcium in the active site, and with calcium bound to a secondary site, whereas we observed the products of ATP hydrolysis (ADP and inorganic phosphate) and a divalent cation in the active sites of the remaining structures. We conclude that in isolation, the ATPase domains of these human Hsp70 isoforms have rather similar properties to those of the previously determined isoforms.
Results and Discussion
A common feature of Hsp70 ATPase domains is that, despite the large conformational changes they are predicted to undergo in their physiological context, their crystal structures are highly similar under different nucleotide conditions. We addressed the question whether this is also true for the NBDs of HSPA1L, HSPA2, HSPA5 and HSPA6. These share between 67 and 92% sequence identity with the NBD of the major stress inducible isoform, HSPA1A (Figure 1). We used a multiconstruct approach [37] to produce the NBDs of these human Hsp70 isoforms for structure determination by X-ray crystallography. We also produced the NBD of HSPA1A, the structure of which has been determined previously [38], [39]. All five proteins were straightforward to produce in E. coli, although the soluble expression levels for HSPA5/BiP were relatively low.
Sequence alignment of the NBDs of selected Hsp70 proteins. Secondary structure elements are indicated for HSPA1A above and for HSPA6 below the alignment. Sequences shown are human HSPA1A (1HJO; gi:5123454); HSPA1L (3GDQ; gi:124256496); HSPA2 (3I33; gi:13676857); bovine Hsc70 (PDB entry 1YUW; gi:76253709), E.coli DnaK (1DKG; gi:16128008); HSPA5 (3IUC; gi:16507237); HSPA9 (no structure available; gi:24234688); and HSPA6 (3FE1; gi:34419635).
Crystals of the five proteins were obtained with NBD constructs that contained either the full length N-terminus or short N-terminal truncations and with ADP and either Mg2+- or Mn2+-ions present in the crystallization solution (Figure 2 and Table 1). Crystals diffracted to a resolution of between 1.8 and 2.2 Å except for the HSPA2 crystals which diffracted to 1.3 Å. The structures were solved by molecular replacement (Table 2). In all ATPase domains except for that of HSPA5/BiP the products of ATP hydrolysis, including inorganic phosphate, were observed. Examples of the electron density around the bound nucleotide are shown for HSPA2 and HSPA5 (Figure 3).
(A) Coomassie-stained SDS-polyacrylamide gel showing the purity of the crystallized proteins. (B-F) Examples of crystals grown under the conditions that yielded the datasets. (B) HSPA1A; (C) HSPA1L; (D) HSPA2; (E) HSPA5; (F) HSPA6.
Representative parts of the 2Fobs-Fcalc density maps around the nucleotide binding site (contoured at 1.5σ above the mean) of (A) HSPA2 with MgADP + Pi, and (B) HSPA5 with CaADP. Selected side chain and main chain atoms within hydrogen bonding distance are indicated.
The overall structures of the four human Hsp70 ATPase domains that were determined here for the first time all closely resemble the structure of HSPA1A and related previously determined structures (Figure 4). The HSPA1A NBD structure determined by us was virtually identical with the HSPA1A NBD structure published previously (PDB entry 1HJO; [39]). The canonical Hsp70 fold, with the common placement of secondary structural elements, was also observed for HSPA5/BiP, the least conserved member of the five proteins studied by us (Figure 4A). Pairwise comparison among these NBDs shows that for ∼70% of the backbone traces the rms difference is below 0.5 Å (Figure 4B). The largest rms deviation in the Cα positions was found for HSPA5, where the overall rms value is higher than 1.3 Å (only 17% of the backbone trace shows an rms difference lower than 0.5 Å). To illustrate this we color coded the rmsd in Cα atom positions between the pairs HSPA6 - HSPA1L, HSPA6 - HSPA5, and HSPA6 - E.coli DnaK, and mapped them onto the structure of HSPA6 (Figure 4C). This analysis shows that the most prominent difference between the HSPA5 and the canonical Hsp70 structures is a shift in subdomain IIB.
(A) The structure of the HSPA5/BiP NBD in complex with CaADP at 2.4 Å resolution. (B) Superposition of the five Hsp70 NBD structures determined in this study. Dark blue, HSPA1A; yellow, HSPA1L; cyan, HSPA2; red, HSPA6; green, HSPA5/BiP. (C) Cartoon representation of HSPA6, colored to illustrate rms differences in Cα-positions between HSPA6 and HSPA1L (left), HSPA6 and HSPA5 (center), and HSPA6 and DnaK (right).
The slightly opened nucleotide binding cleft of ADP-bound HSPA5 distinguishes it from the set of NBDs that crystallized with ADP and inorganic phosphate in the active site. Cleft opening in HSPA5 is brought about by rotation of subdomain IIB by approximately 5.8°, as determined using the DynDom server [40]. This mechanism of cleft opening is similar to that determined by crystallography of Hsp70 proteins in complex with nucleotide exchange factors [32]–[35], but differs from that observed for actin [41].
All five NBDs crystallized in the presence of ADP and either MgCl2 or MnCl2 (Table 1). Inorganic phosphate was present only in the solution for HSPA6. Nevertheless, electron density for inorganic phosphate was observed in the active site of four of these proteins (Table 2), which thus had the products of ATP hydrolysis bound to them. We conclude that at least HSPA1A, HSPA1L, and HSPA2 bound ATP in the expression host, hydrolyzed their bound ATP, and subsequently during crystallization never released the products of ATP hydrolysis into solution. Only the HSPA5 structure contained ADP and metal ion but not inorganic phosphate. Metal ion coordination geometry and distances suggested that here the bound divalent cation was calcium, which was present in the mother liquor. Thus HSPA5 may have lost magnesium and inorganic phosphate after cation exchange for calcium during crystallization.
A second calcium ion was found at the base of HSPA5 α-helix-6, tethered by the H252 hydroxyl and the E256 and D257 carboxylates. This site has previously been identified in human HSPA1 (1S3X) [38]. Conservation of the side chains involved in Ca2+-interactions in human and many other Hsp70s suggests a universal binding site, but to our knowledge no other crystal structures of any Hsp70 protein contain Ca2+ at this site. Given the millimolar concentrations of calcium in the ER we expect that HSPA5 may be partially regulated at the second site found in our crystal structure, whether in relation to ATP turnover or other activities [31]. Interestingly, this second metal binding site is implicated in ATP synthesis by mutagenesis of HSPA1 [42]. ATP synthesis was found to be dependent on both calcium ions and transient Hsp70 phosphorylation [42]. Also, both DnaK [43] and HSPA5 [44] are transiently phosphorylated, in a Ca2+-dependent manner, on the threonine side chain corresponding to HSPA5 T229. We speculate that HSPA5 and possibly other family members might be able to retain the products of ATP hydrolysis for ATP regeneration at a site in close proximity to the active site. The second calcium site might position the ADP pyrophosphate tail for phosphoester bond formation, while phosphorylated T229 (or an alternative side chain in the vicinity, such as strictly conserved HSPA5T251/DnaKT225) might act as phosphate donor. This pathway might be employed in situations of high ATP turnover or low ATP concentrations to secure vital cellular functions.
Materials and Methods
Protein Expression and Purification
The cDNAs coding for the full-length human HSPA1A, HSPA1L, HSPA2, HSPA5, and HSPA6 were obtained from the Mammalian Gene Collection (accession codes BC002453, BC034483, BC001752, BC020235, and BC035665, respectively). The sequence coding for residues HSPA1AM1-N387, HSPA1LM1-K386, HSPA2P6-D386, HSPA5D26-D410, and HSPA6E6-D385 were subcloned into expression vector pNIC-Bsa4 by ligation-independent cloning. The resulting expression constructs contained a hexahistidine tag and a TEV-protease cleavage site (MHHHHHHSSGVDLGTENLYFQS) at the N-termini.
Recombinant expression and purification of the proteins is described in detail on our webpage (http://www.thesgc.org/structures/). Briefly, each expression construct was transformed into E. coli strain BL21(DE3)R3 pRARE (Novagen) and the cultures were grown in Terrific Broth supplemented with 8 g/l of glycerol at 37°C. At an absorbance at 600 nm between 1 and 2 the temperature was lowered to 18°C, recombinant protein production was induced by addition of 0.5 mM isopropyl-β-d-thiogalactopyranoside, and cell growth was continued for 18 h at 18°C. Cells were harvested by centrifugation and the cell pellets were resuspended in 1.5 volumes/wet cell weight of lysis buffer (100 mM HEPES, 500 mM NaCl, 10% glycerol, 10 mM imidazole, 0.5 mM TCEP, pH 8.0, and one tablet of Complete EDTA-free protease inhibitor (Roche Biosciences) per 50 ml cell suspension). Before lysis, 4 µl (1000 U) of Benzonase (Novagen) was added per 50 ml cell suspension, and lysis was achieved by sonication. Cell debris was removed by centrifugation and the soluble fractions were filtered through a syringe filter (0.45 µm pore size). Cleared lysates were passed over 1-ml HiTrap Chelating columns (GE Healthcare) pre-equilibrated with buffer 1 (30 mM Hepes, 500 mM NaCl, 10% glycerol, 10 mM imidazole, pH 7.5, 0.5 mM TCEP). The columns were washed sequentially with buffer 1 and buffer 1 supplemented with 25 mM imidazole. Bound protein was eluted with buffer 1 containing 500 mM imidazole, loaded onto 16/60 Superdex-200 HiLoad columns (GE Healthcare), and gel filtration was performed in buffer 2 (30 mM Hepes, 300 mM NaCl, 10% glycerol, pH 7.5, 0.5 mM TCEP). Fractions were pooled based on gel filtration profiles and purity, TCEP was added to 2 mM, and the proteins were concentrated to 24.8 mg/ml (HSPA1A), 16.0 mg/ml (HSPA1L), 14.0 mg/ml (HSPA2), 31.0 mg/ml (HSPA5A), and 25.0 mg/ml (HSPA6). Proteins were typically more than 90% pure judged by SDS-PAGE analysis (Figure 2A). Protein construct masses were verified by TOF-MS analysis (results not shown). Aliquots were flash-frozen and stored at -80°C.
Crystallization, Data Collection, Structure Solution and Refinement
Crystallization methods and conditions are summarized in Table 1. Crystals appeared after 2–28 days. For data collection crystals were briefly dipped in cryo solution containing 40% PEG 300, 0.15M Na2HPO4, 0.1M citric acid, 0.2M NaCl (HSPA6) or mother liquor containing 15%–20% glycerol (HSPA1A, HSPA1LA, HSPA2, and HSPA5) and flash-frozen in liquid nitrogen.
A single wavelength dataset for HSPA1A was collected with Cu-Kα radiation (1.54166 Å) on an X8 PROTEUM system equipped with a four-circle Kappa goniostat and a PLATINUM-135 CCD (all from Bruker AXS, Delft, Netherlands). SAINT (Bruker AXS) and XPREP (Bruker AXS) were used to process the data. Further datasets were collected at synchrotron beamlines at BESSY (BL14-1), Berlin, Germany (HSPA5 and HSPA6), DIAMOND (I03), Oxfordshire, UK (HSPA1L) and ESRF (ID23-1), Grenoble, France (HSPA2). Data sets were indexed, scaled, and reduced using the programs XDS [45] and SCALA [46]. All structures were solved by molecular replacement using MOLREP [47]. The structures were refined with RefMac5 [48] and model building was done using Coot [49]. For further details on data processing and refinement statistics, see Table 2. Geometry of the models was analyzed with Molprobity [50]. Sequence alignments were obtained using ESPript [51].
Data Deposition
The atomic coordinates and structure factors have been deposited with the Protein Data Bank, www.rcsb.org (PDB entry codes: 3FE1 [HSPA6]; 3GDQ [HSPA1L]; 3I33 [HSPA2]; 3IUC [HSPA5]; 3JXU [HSPA1A]).
Supporting Information
Datapack S1.
Standalone iSee datapack - contains the enhanced version of this article for use offline. This file can be opened using free software available for download at http://www.molsoft.com/icm_browser.html.
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Instructions for installation and use of the required web plugin (to access the online enhanced version of this article).
We gratefully acknowledge the beamline personnel of the BESSY (Berlin, Germany), Diamond (Oxford, United Kingdom), and ESRF (Grenoble, France) synchrotron radiation facilities. We thank Wen Wha Lee (SGC, Oxford University) for assistance in the generation of the iSee datapack (Datapack S1) for the online version of this article.
Author Contributions
Conceived and designed the experiments: MW HS. Performed the experiments: MW TK LL IJ TK MM. Analyzed the data: MW TK LL HS. Wrote the paper: MW HS.
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- Merry #Christmas Everyone!!! Enjoy the day with your family and friends but don't forget those who don't have anyone to celebrate Christmas
http://twitter.com/__CarolineCC/status/415602633060012032— Caroline Cheng (@__CarolineCC)Tue, Dec 24 2013 21:59:23
http://twitter.com/billyslater/status/415606450426109952— Billy Slater (@billyslater)Tue, Dec 24 2013 22:14:34
Australia celebrates Christmas 2013
Take a look at pictures and tweets from around the country as Australia celebrates Christmas.
byABC News1 Like13,601 Views


text:
AfriGeneas States Research Forum
Re: [MS] Slaveowners in Claiborne County
In Response To: [MS] Slaveowners in Claiborne County ()
There were no slaveowners named Mann in Claiborne County, MS in either the 1850 or 1860 Slave census schedules. The nearest Mann listed in a Slave schedule was in neighboring Hinds county in the 1850 census and he was not actually a slaveowner but was actually an overseer, possibly for a C.T. Munis a planter from Virginia. It's pretty complicated stuff and would require quite a lot of research to find if there is a possible connection to your Mann's. Researching slave ancestors is advanced type of research that can take a long time to do and the conventional wisdom is that you need to fully research your family from 1870 to the present before attempting to tackle researching slave ancestors. Good luck.
Messages In This Thread


text:
With back-to-back perfect seasons to start his high school career, Good Counsel's Kyle Snyder has set the area standard for dominance on the wrestling mat. J'Den Cox, a rising senior at Hickman High in Columbia, Mo., has a resume to match with three state titles in as many tries. Thanks to USA Wrestling's junior and cadet national championships, the 220-pound teenagers who live nearly 1,000 miles apart have formed a budding rivalry.
Last week at North Dakota State University's Fargodome, Snyder — the reigning All-Met upper weight wrestler of the year — beat Cox in the semifinals of the junior Greco Roman competition en route to the title. Later, Cox returned the favor, beating Snyder twice to take the freestyle crown.
Those results reversed last summer's showdown in the cadet division when Snyder won the freestyle competition and Cox captured top honors in the Greco Roman tournament.
"We're pretty evenly matched," said Snyder, who was bidding to become Maryland's first double junior champ since Mike Faust in 2000. "He's a tough guy with really good leg defense. He's very aware of his body on the mat."
At one of the country's premier high school tournaments, Cox and Snyder distanced themselves from the rest of the 220-pound field. (The event includes Greco Roman and freestyle tournaments, the styles contested at the Olympics, as opposed to folkstyle, which is used in high school and NCAA competition.)
Matches in both tournaments were scored by period with the first wrestler to win two advancing. For the week, Snyder did not lose any periods in his other 13 contested matches, while Cox lost just one.
Head-to-head, the pair squared off in three tight bouts. Snyder started off strong in the Greco Roman competition, beating Cox in three periods, 0-1, 2-0, 1-0, before knocking off Daniel Chaid of California in the final.
In two freestyle matchups at the end of the week, Cox won all four periods by a 1-0 score. Snyder came back to beat Chaid in the third-place match.
Snyder, 16, will have two more trips to Fargo if he chooses, and he's already looking forward to seeing Cox again.
"If you don't lose, you don't really have anything to fuel your fire," said Snyder. "I don't want to say it was a good thing, but it wasn't a bad thing at the same time."
In the past, Snyder's return from Fargo has signaled the unofficial beginning of his football season. With his focus squarely on wrestling this time around, he plans to take a month off from wrestling to let his body recover and focus on getting even stronger in the weight room.
After a lackluster showing in the Greco Roman tournament, Josh Llopez, a rising junior All-Met, went 11-0 in the junior 152-pound freestyle competition, finishing his run to the title with a 1-0, 2-1 victory over Yoanse Mejia of Florida in Saturday's final.
A year ago in his first trip to the event, Llopez suffered a broken rib early in the week and still managed to finish third in the cadet freestyle tournament. This time, he eased through the Greco Roman portion with a 1-2 record and put much of his energy into bettering that freestyle result.
"You don't go out there to lose," said Llopez, the state's first junior freestyle champ since Faust's double championship 12 years ago. "You train all summer for it. ... Starting on the airplane on the way out there, I was thinking that I was going to win the whole tournament."
Llopez, who has capped his first high school seasons at La Plata with Maryland 4A/3A titles, won't continue that streak. He said he will begin classes at St. Mary's Ryken next month and plans to compete for the Knights next winter in the Washington Catholic Athletic Conference, a move he hopes will help set him up academically to wrestle in college.
"I'm ready to compete with those guys," Llopez said of his venture to the private school scene, which offers the chance to end his season at the National Prep tournament. "I think I can hang."
Recent Centennial graduate Nathan Kraisser — the All-Met lower weight wrestler of the year last winter — finished seventh in both competitions at 126 pounds in his final trip to Fargo. The four-time Maryland public school champion will wrestle at North Carolina next year.
Northern 152-pounder Eric Hoffman — a rising senior who earned second-team All-Met honors last season — was the other local junior wrestler to post two All-American performances with a pair of eighth-place finishes.
Duval 120-pounder Thierno Diallo (fourth), Hayfield 138-pounder Sahid Kargbo (third), Georgetown Prep 138-pounder Daniel Sanchez (sixth) also earned All-American status in the junior Greco Roman competition.
In the cadet category (ages 14-15), Severn 88-pounder Michael Doetsch earned All-American status with top-eight finishes in both competitions, including a second-place showing in freestyle. Northern 88-pounder Breandan Coughlin (second) and Good Counsel 94-pounder Adam Whitesell (eighth) were Greco Roman All-Americans.
On the girls' side, Harriet Symington, a rising junior at Whitman, posted a pair of All-American performances, finishing third in the cadet freestyle competition at 101 and seventh in the junior freestyle draw at 97.


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Date: Mar 26, 2016 | Time: 10:30 am - 12:30 pm
Egg-stravaganza Hunt at North County Recreation Complex. For children 5-11. Meat at the Leo Wilson shelter and playground. Advance registration is required by calling 314-615-8839. Cost is $5 per child.
For More Information:
Call for Reservations | 314-615-8839 | Website
North County Recreation Complex is located in Veterans Memorial Park and features an Olympic-size outdoor swimming pool, a playground, tennis courts, a fishing pond, a picnic shelter, a gymnasium, and includes the Eagle Springs Golf Course.
Directions: Located on Redman Road in Veterans Memorial Park. From Interstate 270, go north on Old Hallsferry Road and make a right onto Redman Road to entrance to Park.
Full Service Real Estate Brokerage! I have been helping area buyers and sellers for 28+ years! See More Info
314-332-1010 | 314-503-4663


text: I found this place online. As always, I was a bit leary of booking online. I was looking for a quiet little place to take my boyfriend away for his birthday. Away from cell phones, computer, work, etc. We both love the ocean and I was really hoping to find an ocean front room that was reasonably priced. Cape Ann... More


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April 7, 2014
Pet of the Day
Coco Knibs
Two and a half years old
Guinea Pig
Wisconsin, USA
ell, I got Coco Knibs, just Coco for short, on Christmas 2011. She is around two and a half years old now, I do not know her exact birthday. She has a cute personality, and she loves to have cuddle time. She also likes it when I take her down into the kitchen with me where she'll explore. She loves going outside too, and we had one of our adventures when we were out there; like the time when a tree fell down, and I let her crawl in and out of it (it was hollow). Well, one time she wouldn't come out, and I was trying to get her out, but wouldn't for over an hour. But thankfully she came out eventually! :)
Coco also likes to explore our flower beds. She has fun doing that, although she can get grubby! Coco has her little way of letting me know when she has to go to the bathroom. When I'm holding her, she'll start crying a little, letting me know she has to go so we can make it back to her cage. Overall, she is my best animal friend I've got :)!
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