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Lyndal needed a new logo design and created a contest on 99designs.
A winner was selected from 245 designs submitted by 42 freelance designers.
Brand Expressions
Brand Expressions are an innovative retail-marketing agency specialising in brand development for up-market fashion, décor, beauty and lifestyle retailers & shopping centres.
We improve your customers experience by using a mix of above-the-line, below-the-line, in-store and promotions-based marketing, resulting in enhanced brand awareness and increased revenue.
Up-market fashion, décor, beauty and lifestyle retailers & shopping centres.
We are looking for a logo for our Retail Marketing Agency.
1. The logo must be up-market, simple, clean, bold & have a touch of femininity.
2. We would like it to in corporate a butterfly but not to look like a lifelike butterfly
3. Colour: We like strong neutrals with a touch of colour
4. Example of logo we like http://www.huttonsdirect.com/. What we like about this is the colour, font, and the flower logo, if cut in half, can be manipulated to be a butterfly
The files we will require are:
Print file format - .ai (CMYK)
Web file format - .jpg and layered .psd (RGB)
Please do not use any stock images or clip art in the logo.
Thanks so much and we look forward to receiving your designs!!!
Find out how a design contest works or learn more about our logo design services.


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Nyctalops is a fanzine edited by Harry O. Morris and Edward Paul Berglund and published in the 1970's.
Nyctalops is devoted to H.P. Lovecraft and his circle, Charles Ashton Smith, and Robert Bloch. It is a 8.5 X 11" publication of approximately 100 pages that was published in Albuquerque, New Mexico, U.S.A. The first issue appeared in May 1970. Nyctalops was initially published solely by Morris and consisted of literary criticism. but after issue three he asked Edward Berglund to become fiction editor for the zine and commencing with issue four the fanzine included fiction. The two editors worked together for seven more issues, with nineteen issues released in all.
Contributors included Bernadette Bosky, David J. Brown, Ramsey Campbell, Walter C. DeBill, Jr., Gary Drake, Denise Dumars, Thomas M. Egan, Steve Eng, R. Alain Everts, Kenneth W. Faig, Jr., Donald Sidney-Fryer, Charles Richard Grose, Alan D. Gullette, Gary Harrison, John Jacob, John Koblas, George Laking, James Lawson, Thomas Ligotti, Dirk W. Mosig (The Miskatonic), Joe Moudry, Gerald W. Page, Ted Pons, E. Hoffman Price, Graham Pryor, W. Pugmire (Midnight Fantasies, Old Bones), Phyllis Rose, Jessica Amanda Salmonson (The Literary Magazine of Fantasy and Terror),Darell Schweitzer, Vernon Shea, James Taylor, Stephen Verba, Bruce Walker, William Wallace, George Wetzel, and Neal Wilgus.
Artists included Mike Garcia, Jim Pitts, Denis Tiani and Helmet Wenske.

subset: commoncrawl


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Lady Gaga Uses Dress to Mock Critics of Her Weight Gain
Lady Gaga left her hotel in Paris -- amid a fan and paparazzi frenzy, of course -- wearing a crazy-looking pink and blue dress that required her to keep her arms outstretched. It also made her look several sizes larger, perhaps in response to all the media coverage of her recent weight gain, which even had some speculating that she was pregnant with her first child. Slow news week, huh?
The dress was shapeless, and she wore it with goggle-like glasses and shoulder-length brown hair. We're going to speculate that it's her real hair, given the length. She usually always wears wigs, but the shortness of her hair while exiting her hotel made us think it's her natural locks. She paired the overall look with fuchsia lipstick. The strange frock left many wondering if it was her way of responding to the overflow of media reports about her size. It could have been a case of "If you can't beat 'em, join 'em."
Gaga had gone on record to say that she packed on pounds thanks to the fact that she was noshing on her dad's Italian cuisine at his Italian restaurant Joanne. Good plug, Gaga!
Check out the photos of here. What do you think of her statement? Do you think she's really gained that much weight? Tell us in the comments!


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So you've been to Federation Square, Crown Casino, Docklands and taken a trip on a Melbourne tram. Some of the best ways to learn about and explore the intricacies of the city and its culture are those you'll find off the beaten tourist track.
I'm amazed it was free!
Trek the Noojee Trestle Bridge Rail Trail for a step back in time to surround yourself with history and discover the hidden treasures that lie beyond the road.
Be a part of the local audience in one of the many television shows produced in Melbourne. For example, Millionaire Hot Seat and The Project are free of charge but be sure to check the websites and email well ahead, as the seats fill fast.
Take a trip to the open air Queen Victoria Market. With its old world design and heritage, you don't need to spend a cent to enjoy this iconic market's history, although you will likely be tempted by the exotic and tasty produce and bargain merchandise the many shops and stalls offer.
For the price of a cup of coffee
Are you Feline lonely? Then visit Australia's first cat café! Soak up the 'catmosphere' in this conceptual cat'fe (the puns are endless…) while hanging out with affectionate felines sitting next to you or if you're lucky, on your lap. For cat lovers, there is possibly no better way to spend a relaxing holiday afternoon than with these 11 rescue cats just waiting for your attention.
The small price of a 45 minute bus fare from the city's CBD buys you a trip to the Royal Botanic Gardens in Cranbourne. Once there, there is no cost of entry and because of the Melbourne's notoriously rainy climate, you will find some of the most luscious and distinctive native flora the country has to offer. The gardens are open from 9am to 5pm daily.
Or for just a little more…
Try one of Laneway learning's fun and cheap classes on a wacky range of topics. Originally operating only in the lanes of Melbourne (hence the name) the popularity and novelty factor has meant that they have now been able to expand into cafés and bars. Check the website for upcoming details which may offer exciting classes in the diverse areas of drawing, chess, hair styling and cookery. Reserve your place well in advance because the most popular classes do tend to fill very quickly.
If it takes your fancy you could learn how to dance 1980s style and frankly, you know you want to right? Whether you think you're hip to be square or solid gold, you will probably giggle your way through the whole groovy class!
Would you like to play a game? Consider escapism to the max in a room at Strike by joining one of many likeminded people or create your own team with friends and family. From perplexing puzzles to spy hunts, crime scene investigations and trapping interactive scenes there are a number of games you can play. Choose your poison, wisely.
Find your artistic creative style by participating in a sketch crawl of the city. On a guided tour, you will be taken to a variety of walking and drawing sights to inspire your inner artist.
But you saved a lot of money on your accommodation so why not splash out?
Do it literally by having supper with the sharks. Working your way from stingrays while you eat your entrée to leopard and white spotted sharks during your main meal this is a feast you won't forget. But remember, even though the water may only come up to your knees, those rays do like to be fed so if something falls out of your prawn cocktail, don't bother trying to pick it up off the floor. Oh, and apparently stingrays are also known to like a good cuddle.
Or if you are up for a road trip, hire a car and visit Werribee Open Range Zoo. From roaring lions to (overly) friendly rhinos and gorillas who just want to hang out with you for afternoon tea, this zoo has some special attentive treats in store. Visit the Werribee Open Range Zoo to book your expedition!
Spend Less, Do More
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Hire a car from us and get exploring!
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Tada! We finally were able to get our new shiny site up and running!
Don't worry, we did move all of our posts there too!
From now on please check out WWW.BUBBLEPUNCH.COM to keep up to date to all of our events, as well as our services, sightings, and fun filled adventures!


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Whether they're traditional trunks, swim shorts or board shorts, men's swimwear comes in a wide variety of styles, colors and patterns to suit anyone's tastes and physiques. Here's how to decide what will look best on you whether you're at the pool or on the beach.
What is your body type?
Are you thin and tall, well-muscled or are you a bit on the stocky or round side? You'll want to choose the most flattering cut depending on your physique. If you're tall and thin, you may look good in classic boardshorts. Men who are big and tall should choose men's beachwear that's darker in color and not too tight or too baggy.
What do you want to do?
If you plan on being active – such as surfing, swimming or playing beach volleyball – you'll want a different type of men's swimwear than if you were spending the day lounging in the sun.
What do you feel comfortable in?
Some guys like super-baggy board shorts while others prefer more form-fitting swim trunks. Try on a couple of different types to see what is right for you.
How many will you need?
Again, this is a personal preference. If you only go in the water a couple of times a year, then you can get away with one or two swimsuits; if you're regularly swimming, surfing or participating in other water sports, then you'd probably want to own three or four bathing suits each season.
Make this the best beach season yet with a wide range of men's swimwear and beachwear made in the USA at BeallsFlorida.com.


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Teresa Norris 8/31/2006 8:34:46 AMBrian, great image! Love the colors and her cute expression! Great comp. too! She's adorable. #513819
Boentara Prajitno 8/31/2006 8:45:18 AMlovely #3249724
Brenda c. McFarland 8/31/2006 9:30:33 AMVery Cute. She reminds me of my little pumpkin. #3249885
Kay E. Mahoney 8/31/2006 11:35:44 AMVery cute. Love her expression. #3250539
Colette M. Metcalf 8/31/2006 12:15:36 PMCute capture!!! #3250710
Rhonda Burger 8/31/2006 12:45:14 PMGreat job! I love the composition. #3250866
Glenda H. Travis 8/31/2006 4:53:52 PMOK this is a great capture..it made my giggle!
Kay E. Mahoney 9/26/2006 4:42:12 PMCongratulations on your finalist. Good luck in the next round. #3365705
William88 9/26/2006 5:03:36 PMCongratulations on your finalist. #3365959
Wendy Stevenson 9/26/2006 6:10:19 PMCongrats on this wonderfully refreshing finalist!! Great image! #3366923
Debbie Vega 9/26/2006 8:11:49 PMBrian, I love this image, her expression and the colors are amazing.. great job.. debbie #3368424
Teresa Norris 9/26/2006 8:36:50 PMI'm so happy to see this again! Wonderful...good luck in the next round1 #3368689
Sue L. Cullumber 9/27/2006 6:48:33 PMBrian, Great shot - you are so brave, I'd be afraid to get my camera wet! Congrats! #3376279
RC Fritz 9/27/2006 7:05:53 PMGreat capture. Congrats! #3376351
Janine Russell 9/29/2006 1:01:41 AMBrian, congratulations on your Second Place win. #3383351
Kay E. Mahoney 9/29/2006 6:28:49 AMCongratulations on your 2nd place winner. #3384909
Jane M 9/29/2006 1:17:52 PMGreat color and a worth second place! #3387018
Wendy Stevenson 9/29/2006 2:51:45 PMGreat to see this in the winners circle Brian - really fun image!! #3387345
Susana Ms Heide 9/29/2006 9:18:19 PMThis is such a FUN image!!! Congrats on your 2nd place win!!! #3388794
Taken in Louisville ky, ny daughter gracie cooling off under a refreshing sprinkler. 1/250 f8 400 taken with my 24-70
Uploaded on 8/31/2006 8:31:45 AM
Camera: Canon EOS 10D Digital SLR Camera
Copyright for this gallery photo belongs solely to Brian R. Steffen. Images may not be copied, downloaded, or used in any way without the expressed, written permission of the photographer.
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About the Authors: Ambassador Philip L. Verveer serves as U.S. Coordinator for International Communications and Information Policy at the U.S. Department of State, Lawrence E. Strickling serves as Administrator of the National Telecommunications and Information Administration (NTIA), and Julius Genachowski serves as Chairman of the Federal Communications Commission (FCC).
On the eve of the World Conference on International Telecommunications (WCIT), we believe that it is the right time to reaffirm the U.S. government's commitment to the multi-stakeholder model as the appropriate process for addressing Internet policy and governance issues. The multi-stakeholder model has enabled the Internet to flourish. It has promoted freedom of expression, both online and off. It has ensured the Internet is a robust, open platform for innovation, investment, economic growth and the creation of wealth throughout the world, including in developing countries.
There are those who may suggest next week in Dubai -- and in future venues where Internet policy is discussed -- that the United States controls the Internet. Alternatively, they may suggest that in the future governments alone should run the Internet. Our response is grounded in the reality that this is simply not the case. The Internet is a decentralized network of networks and there is no one party -- government or industry -- that controls the Internet today. And that's a good thing.
The Internet's decentralized, multi-stakeholder processes enable us all to benefit from the engagement of all interested parties. By encouraging the participation of industry, civil society, technical and academic experts, and governments from around the globe, multi-stakeholder processes result in broader and more creative problem solving. This is essential when dealing with the Internet, which thrives through the cooperation of many different parties.
The global community has many serious topics to discuss with respect to the Internet. Collectively, we need to ensure that these matters are taken up in suitable multi-stakeholder venues so that these discussions are well informed by the voices of all interested parties.
Our commitment to the multi-stakeholder model is based on the fact that transparency, inclusion and participation are the 21st century standards governing discussions related to modern communications. This is a view shared by many around the world and was most recently reiterated by a statement of civil society members and groups from around the world who participated in the "Best Bits" pre-Internet Governance Forum (IGF) meeting held earlier this month in Baku, Azerbaijan. The U.S. government wishes to lend its support to the spirit of the recommendations contained in the statement.
We have and will continue to advocate for an Internet that is not dominated by any one player or group of players, and one that is free from bureaucratic layers that cannot keep up with the pace of change. We will work with everyone to ensure that we have a global Internet that allows all voices to be heard.
W.W. writes:
there is no access to international data banks on search engines - gov can filter informations
Marion M. in the U.S.A. writes:
Too bad you don't practice what you preach! Your post above about a free and open internet not controlled by any one entity is to be applauded. Why is that openness not applied when it comes to what choices you recommend for people setting up their Google page? I decided to add your politics group to my Google page and was amazed at how very one sided you are. Not only do you not initially offer any different views but you seem to slam FOX news and all Republicans. You do not even make an attempt at covering your bias! You do not even make a stab at being balanced.
I find this incredibly funny as it is people you seem to love that is trying to shut you up. The UN wants to control the internet....and the world.....Agenda 21 comes to mind along with their stance on internet freedom.
Your attempt at controlling what young people ingest as news is so slanted to the left! I like to read both sides and I also know when I'm being fed biased thought. It would be refreshing if you just reported the news and also made readily available all different channels of thought and news reports to choose from. Stop pushing your leftist agenda and try being neutral. That's what the defense of freedom and this country truly need!


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This Positive Life: An Interview With Wanda Hernandez
March 1, 2011
When Wanda was diagnosed with HIV in 1995, she was completely shocked; she thought she had always practiced safer sex. Like many people who are newly diagnosed, fear began to take over. But instead of letting that fear consume her, she educated herself about HIV and eventually became an activist lobbying in Albany, N.Y., on behalf of people living with HIV/AIDS. This mother of one talks about the loneliness of being positive and working in corporate America, the importance of securing housing for people living with HIV/AIDS, and why the AIDS community can never give up hope.
Welcome to This Positive Life. I am here today with Wanda Hernandez. Wanda, Welcome to This Positive Life.
Thank you Kellee and welcome to my home.
It's so lovely and thank you for having me. Let's start by describing how you found out that you were HIV-positive.
I was diagnosed in 1995. I became ill because I am asthmatic and I had a high fever that just would not go away. This happened six months from having had sexual contact with someone who either didn't know or didn't care about his quality of life. I found out after being hospitalized and having every test in the book, that I was positive.
How old were you then?
I was 35.
What had you known about HIV before you were diagnosed?
Before I was diagnosed, I knew about protection and people being able to lead a normal life. About the medications that were out and basically, I was somewhat about HIV educated before I found out that I was positive.
When you heard the words, "Wanda you are positive," what did you think?
Like every other person who is newly diagnosed, I was devastated, like, "Wow, my life is over." But my life isn't over. I found that out, through my education. I decided to fight back, get educated and find out all the things that I need to know in order to survive. Because you could lead a normal life -- I have learned for the past 16 years. I tell people: Don't give up, because there is life after HIV.
So let's go back to that day that you tested positive. You said you were devastated. Walk me through those moments. Were you like, "When should I start treatment?" Or were you like, "Oh my God, I have to get out of here."
I felt more like the "Oh my God, this cannot be happening to me." I mean, I was such a cautious person. But you always think that "This is not going to happen to me." But, it wasn't about the diagnosis; it was more the way it was presented to me when it was given to me. I found it to be a little bit harder because of how it was presented to me.
Actually, I got my diagnosis at a facility that is supposed to be Catholic oriented and the one person whose job it was to give my diagnosis, I didn't feel was too professional about it.
"Like every other person who is newly diagnosed, I was devastated, like, 'Wow, my life is over.' But my life isn't over."
You didn't think he was compassionate at all?
No, not the way he presented it.
So, after that incident, where did you go to get care?
Well, I didn't go there, but I did go and get a second opinion. It's always important to get a second opinion because mistakes do occur. So I went to, unfortunately Southview Clinic where Senator Espada -- of course that is his clinic. But there as well, yes I found out that the diagnosis was correct, I was put on medication right away. Unfortunately, I didn't know at the time that you could hold off on the medication -- it's a choice you take -- but being that I was new at it and did not know what to do and was depending on my doctor and her advice, I started on the meds right away.
I've been, today until 2011, I have always been at a level considered 100 percent healthy, my CD4 count has always been over 1000 and I have always been undetectable.
So has that been the first HIV test you had ever had?
When I was first diagnosed, yes. When I got the second test, of course that was the second time. Same year of course, but that had been the only one. I didn't feel that I was at risk, which I guess was a big mistake because even of course in our sexual orientation, or what you do, you always use your protection, but things can occur no matter what.
Did you didn't think you were at risk?
Not that I didn't think that I wasn't at-risk , more like I didn't think it was going to happen to me because I was extra careful.
So how do you think you knew how you became positive?
I know exactly how I became positive -- it was sexually transmitted. There was protection, it was actually someone I trusted and gave the chance to have something to do with. And I feel that the mistake when the person might have taken the condom, or took it the next level by deciding that he was going to remove the condom and not put it back and not let me know. And things happen in less than a second and that's how it goes.
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Bley / Koglmann / Peacock
Search for other titles on the Hatology label.
The trio of bassist Gary Peacock, pianist Paul Bley, and trumpeter Franz Koglmann interpret and improvise on the music of avant song-writer Annette Peacock.
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Label: Hatology
Catalog ID: Hatology674
Squidco Product Code: 13409
Format: CD
Condition: New
Released: 2010
Country: Switzerland
Packaging: Cardstock 3 page foldover
Recorded on April 12th through the 14th of 1992 at Radio Bremen, Germany by Peter Schulze and Volker Steppat. Originally released on CD in 1992 as hat ART CD 6118.
Paul Bley-piano
Franz Koglmann-flugelhorn, trumpet
Gary Peacock-doublebass
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and click here to Search for albums with that instrument.
Artist Biographies:
• Show Bio for Paul Bley
"Hyman Paul Bley, CM (November 10, 1932 - January 3, 2016) was a Canadian pianist known for his contributions to the free jazz movement of the 1960s as well as his innovations and influence on trio playing and his early live performance on the Moog and Arp audio synthesizers. Bley was a long-time resident of the United States. His music has been described by Ben Ratliff of the New York Times as "deeply original and aesthetically aggressive." Bley's prolific output includes influential recordings from the 1950s through to his solo piano records of the 2000s.
Bley was born in Montreal, Quebec, on November 10, 1932. His adoptive parents were Betty Marcovitch, an immigrant from Romania, and Joe Bley, owner of an embroidery factory. However, in 1993 a relative from the New York branch of the Bley family walked into Sweet Basil in NYC and informed him that his father was actually his biological parent. At age five Bley studied violin, but at age seven he decided to switch to the piano. By eleven he received a junior diploma from the McGill Conservatory in Montreal. At thirteen he formed a band which played at summer resorts in Ste. Agathe, Quebec. As a teenager he played with touring American bands, including Al Cowan's Tramp Band. In 1949, when Bley was starting his senior year of high school, Oscar Peterson asked Bley to fulfill his contract at the Alberta Lounge in Montreal. The next year Bley left Montreal for New York City and Julliard.
In the 1951, on a return trip to Montreal, Bley organized the Jazz Workshop with a group of Montreal musicians. In 1953 Bley invited the bebop alto saxophonist and composer Charlie Parker to the Jazz Workshop, where he played and recorded with him. When Bley returned to New York City he hired Jackie McLean, Al Levitt and Doug Watkins to play an extended gig at the Copa City on Long Island. In 1953 the Shaw Agency booked Bley and his trio to tour with Lester Young, billed as "Lester Young and the Paul Bley Trio" in ads. He also performed with tenor saxophonist Ben Webster at that time. He then conducted for bassist Charles Mingus on the Charles Mingus and His Orchestra album. Additionally, in 1953, Mingus produced the Introducing Paul Bley album for his label, Debut Records with Mingus on bass and drummer Art Blakey . (In 1960 Bley recorded again with the Charles Mingus Group.)
In 1954 Bley received a call from Chet Baker inviting him to play opposite Baker's quintet at Jazz City in Hollywood, California for the month of March. This was followed by a tour with singer Dakota Staton.
Down Beat Magazine interviewed Bley for its July 13, 1955 issue. The prescient title of the article read, "PAUL BLEY, Jazz Is Just About Ready For Another Revolution." The article, reprinted in Down Beat's 50th Anniversary edition, quoted Bley as saying, "I'd like to write longer forms, I'd like to write music without a chordal center."
Bley's trio with Hal Gaylor and Lennie McBrowne toured across the US in 1956, including a club in Juarez. Mexico. The tour culminated with an invitation to play a 1956 New Year's Eve gig at Lucile Ball and Desi Arnez's home in Palm Springs. During the evening, Bley collapsed on the bandstand with a bleeding ulcer and Lucy immediately took him to the Palm Springs hospital where she proceeded to pay for all of his medical care. Bley, who had met Karen Borg while she was working as a cigarette girl at Birdland in NYC, married her after she came out to meet him in Los Angeles, where she became Carla Bley.
In 1957 Bley stayed in Los Angeles where he had the house band at the Hillcrest Club. By 1958 the original band, with vibe player, Dave Pike, evolved into a quintet with Bley hiring young avant garde musicians trumpet player Don Cherry, alto saxophonist Ornette Coleman, bassist Charlie Haden and drummer Billy Higgins.
In the early 1960s Bley was part of "The Jimmy Giuffre 3," with Giuffre on reeds, and Steve Swallow on bass. Its repertoire included compositions by Giuffre, Bley and his now ex-wife, composer Carla Bley. The group's music moved towards chamber jazz and free jazz. The 1961 European tour of The Giuffre 3 shocked a public expecting Bebop, however the many recordings released from this tour have proven to be classics of free jazz. During the same period, Bley was touring and recording with tenor saxophonist Sonny Rollins, which culminated with the RCA Victor album Sonny Meets Hawk! with tenor saxophonist Coleman Hawkins. Bley's solo on "All The Things You Are" from this album has been called "the shot heard around the world" by Pat Metheny.
In 1964 Bley was instrumental in the formation of the Jazz Composers Guild, a co-operative organization which brought together many free jazz musicians in New York: Roswell Rudd, Cecil Taylor, Archie Shepp, Carla Bley, Michael Mantler, Sun Ra, and others. The guild organized weekly concerts and created a forum for the "October Revolution" of 1964.
In the late 1960s, Bley pioneered the use of the Arp and Moog synthesizers, performing live before an audience for the first time at Philharmonic Hall in New York City on December 26, 1969. This "Bley-Peacock Synthesizer Show" performance, a group with singer/composer Annette Peacock, who had written much of his personal repertoire since 1964, was followed by her playing on the recordings Dual Unity (credited to "Annette & Paul Bley") and Improvisie. The latter was a French release of two extended improvisational tracks with Bley on synthesizers, Peacock's voice and keyboards, and percussion by Dutch free jazz drummer Han Bennink, who had also appeared on part of Dual Unity. [biography continues...]"-Wikipedia (https://en.wikipedia.org/wiki/Paul_Bley)
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• Show Bio for Gary Peacock
"Gary Peacock (born May 12, 1935, in Burley, Idaho, United States) is an American jazz double-bassist.
After military service in Germany, in the early sixties he worked on the west coast with Barney Kessel, Bud Shank, Paul Bley and Art Pepper, then moved to New York. He worked there with Bley, the Bill Evans Trio (with Paul Motian), and Albert Ayler's trio with Sunny Murray. There were also some live dates with Miles Davis, as a temporary substitute for Ron Carter.
Peacock spent time in Japan in the late 1960s, abandoning music temporarily and studying Zen philosophy. After returning to the United States in 1972, he studied Biology at the University of Washington in Seattle, and taught music theory at Cornish College of the Arts from 1976 to 1983.
In 1983 he joined Keith Jarrett's "Standards Trio" with Jack DeJohnette (the three musicians had previously recorded Tales of Another in 1977 for ECM Records, under Peacock's leadership). Among the trio's albums are Standards, Vol. 1 and Standards, Vol. 2 and Standards Live.
With the breakup of the "Standards Trio" in 2014, Peacock decided to continue his career as the leader of his own piano trio, with Marc Copland on piano and Joey Baron on drums. His 80th birthday year (2015) saw him touring worldwide with this trio to support their ECM release."-Wikipedia (https://en.wikipedia.org/wiki/Gary_Peacock)
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^ Hide Bio for Gary Peacock
1. Touching 1 5:52
2. El Cordobes 8:31
3. Cartoon 6:26
4. Albert's Love Theme 3:46
5. Kid Dynamite 4:26
6. Miracles 6:47
7. Blood 1 5:03
8. Annette 6:26
9. Both 4:26
10. Blood 2 2:46
11. Mister Joy 8:37
12. Touching 2 1:45
sample the album:
"Inspiration, elusive and ethereal, can come from anywhere. Once considered a divine influence, allowing the chosen one to receive a sacred revelation, it can result from common or surprising, rational or illogical sources. Much "art" is (and has been) created without the spark of inspiration, from mechanical or technical motivation. It's not always easy to tell the difference. When true inspiration does occur, it may be a response to either technical demands or emotional stimuli - in either case, heightening the energy and intensity conducive to communication.
Though seldom revealed, feelings this vulnerable and explicit are real, music this honest and open is real, and the inspiration here is palpable and real."-Art Lange, from the liner notes
At The Squid's Ear!
Hat Art
Improvised Music
European Improvisation and Experimental Forms
Search for other titles on the Hatology label.


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Brandywine Hall Rm 003, Ground Floor
709 S. New Street
West Chester, PA 19383
Phone: 610-436-2660
Fax: 610-738-0591
Email: email@example.com
Richard A. Jackson
ResNet Central Manager
Looking to buy your student a new computer? Need a little help?
90% of our campus uses Windows. However, if you are looking to buy a new machine, your major can help determine your choice. Departments such as Music, Art, and Education use MACs and have software to run on MACs. Other departments use Windows PCs. However, any department that uses MACs also has a MAC lab, which means, even if you don't own a MAC, you can use one.
NOTE: Technical support for students with MAC computers is only available through ResNet Central.
|Computer Part
|Operating System
|Windows 7/Windows 8/Windows 10 (Basic, Home, Premium, Ultimate Editions)
|Industry standard processor (Intel Core 2 Duo Processor or higher, AMD Athlon 64 X2, AMD Turion 64 X2, etc.)
NOTE: 32 bit systems cannot use all of 4 GB of memory, so unless you have a 64-bit machine, getting the extra GB will not give you the performance boost you are looking for.
|Hard Drive
|300 GB minimum / at least 500GB recommended
|Wireless capability
|CD and/or CD/DVD combo drive with CD burning capabilities
All of the software recommended or required is available for free download at the ResNet Software page.
For current WCU students Microsoft Office is available for free download.
Many anti-virus programs include anti-spyware. We use Microsoft Security Essentials, which does include anti-spyware. It is free and can be downloaded from our ResNet Software page. Windows 8 and later come with a version of Defender which is now a full fledged antivirus program.
IMPORTANT!!!!! - NEVER install multiple antivirus programs! This could damage your drive and destroy your data!


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(CNN) -- For award-winning Nigerian film director Obi Emelonye, the London premiere of "Last flight to Abuja" in early June was supposed to be a celebratory event, a marquee moment introducing his suspense-filled airplane disaster thriller to the rest of the world with pomp and grandeur.
But then June 3rd happened.
On that fateful Sunday, the Dana Air Flight 992 from the Nigerian capital of Abuja crashed into a densely populated neighborhood in Lagos, killing all 153 people aboard as well as at least 10 people on the ground.
The tragic news left Emelonye, whose high-octane action movie is based on a series of fatal air crashes that stunned Nigeria in 2006, in a state of shock.
"The coincidences and the timing of it was scary," he says. "This was five days to the London premiere that we've been building up to -- my first reaction was to cancel the premiere."
But after consulting his team, Nigerian officials and some of the families of the bereaved, Emelonye was convinced to go ahead with the original plan, using the movie to spotlight aviation safety in Nigeria.
"They said 'no, this might end up being one of the longest lasting legacies to the lives of these people that were lost so needlessly in those crashes,'" he remembers. "This film was supposed to flag some of those issues that have now taken their lives, so it's in their interest that this story goes out there -- if for nothing, to put the issue of aviation safety squarely in the public agenda so that we don't forget."
Five days later, in an emotional event attended by hundreds of people, Emelonye made sure the premiere was dedicated to the Dana Air crash victims -- attendees observed one minute of silence while the film's end credits were replaced by the names of those who lost their lives on that ill-fated flight.
Emelonye says that "Last Flight to Abuja," written in 2007 and shot in November last year, has now become a campaign film, raising attention for safer flying in Nigeria and the rest of the continent.
"The film has taken on added significance way beyond my planning," he explains. "It has become an advocacy ... for aviation safety, not just in Nigeria but for the whole of Africa, and it's a responsibility I take very seriously."
As a result, Emelonye says that some of the profits of the film will be donated to a fund dedicated to helping the families of the air crash victims.
"We're trying to give back financially because we feel whatever profits from this film should go to, in some way, to continue the campaign for safer skies," he says. "In the absence of social security, there are people in dire hardship from that accident and we'll contribute something and kind of compel our partners to contribute also."
At just 30 years old, Emelonye is one of the rising stars of Nigeria's booming movie-making industry, known as Nollywood. Passionate and self-taught, he left behind a career in law to follow his dream of becoming a filmmaker. He achieved critical and commercial acclaim last year with "The Mirror Boy," a fantasy/adventure film released all across the African continent and the UK.
And now he is aiming for further success with "Last Flight to Abuja," a big-budget production starring many of Nollywood's biggest names.
The 81-minute long film has already become a box office hit in Nigeria and is shown in screens across West Africa and in London.
Emelonye says he hopes the movie, along with promoting civil aviation safety in Nigeria, will open up his country's burgeoning film industry to a wider audience, dismissing the low-quality tag that's often attached to Nollywood productions.
"We have a film that has pushed the boundaries with Nollywood and introduced a new genre in Nollywood filmmaking," he says.
Emelonye says Nollywood films are growing in popularity because they offer audiences a narrative they can connect to.
"There's something I call the quintessential African voice, which has kind of given Nollywood, in spite of its qualitative problems, international attention -- it's watched across Africa, it's watched across the world, even though they're shot on very low quality cameras, very simplistic stories, they have a common connection with people that is great."


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Home » Eastern Europe » AVRAM, ANA MARIA/ DUMITRESCU, IANCU/ iO String Quartet
AVRAM, ANA MARIA/ DUMITRESCU, IANCU/ iO String Quartet
Four radical pieces for a great - and young - American string quartet who give life and power to four intense compositions (3 for quartet, one for quartet and tape). Spectralism is slowly becoming more accepted and located in the listening world, and the reduced nature of a string quartet, combined with the great sensitivity of these players, urges minute concentration on the part of both performers and the listeners (digital silence also helps) which is repaid in an edge-of-seat, time-locked existentiality. The four pieces move in different directions and are saturated with detail, the compositions seeming to rise out of the grain of the performance, rather than being an anachronous imposition upon it - a tribute to the vision and skill of the composers, who tread a perilous path between precision and dependency - demanding immediacy through prevision, open scoring and will; a dangerously ambiguous endeavour that erases the power relations between individuality and collectivity at the point of realisation. There are three pages of clunking text by Ben Watson in the accompanying booklet that, to this reader, seem completely inappropriate - an incomprehensible antithesis to the subtlety, depth and sensitivity of the music. Unhappily the delivery of this text as part of the concert is included as an unannounced final track on the CD. toe-curlingly inappropriate. But skip-able.
Code: EDMN1025
Price: £14.50


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Last week, a Los Angeles County Superior Court judge ordered the Irwindale plant that produces the highly popular "rooster sauce" Sriracha to cease operations that could be emitting odors that are "extremely annoying, irritating and offensive to the senses warranting consideration as a public nuisance."
Too bad the judge didn't apply the same logic to the city government of Irwindale and shut it down too.
A brief introduction to the conflict: On one side is David Tran the immigrant founder and CEO of Sriracha's maker, Huy Fong Foods, who claims the rooster sauce (made of chili peppers, distilled vinegar, garlic, sugar and salt) is being scapegoated and worries (along with Sriracha's many fans) that court action will threaten the company and its precious sauce supplies. On the other side are Irwindale city officials, who sought the judge's order and claim the Huy Fong plant is producing smells that burn throats and cause coughing fits, gagging, and even nosebleeds.
But the stench of Sriracha has obscured a far more toxic California problem at play in the dispute: our state has far too many local governments for its own good.
Irwindale, one of California's more than 6,000 local governments, is not so much a city as it is a playland for 700-some businesses. The city has nearly 30 times more workers (40,000) than actual residents (1,400), and residential neighborhoods take up less than one percent of its 9.5 square miles.
Irwindale is not alone in having such odd proportions. Vernon, a city of 112 people and 1,800 businesses, is so relentlessly corrupt that Assembly Speaker John Perez championed legislation to disincorporate it two years ago. Vernon fought off the municipal death penalty by buying off opponents.
Small California cities are often the sites of disputes between small local factions over the city's spoils of prosperity — jobs, development incentives, city contracts, special services. Occasionally, outside authorities — a county district attorney or an air quality regulator — intercede to stop the worst cases of corruption, but they invariably retreat without resolving the larger structural problem. California has little in the way of government at the regional level, and its state government is distant and out of touch. So no one can rein in the Irwindales. Instead, California local governments compete to exploit loopholes in the state's dysfunctional budget system. Those with the fewest scruples turn those loopholes into business incentives, lavish salaries for public employees, and perks for residents. It's no accident that two current and two former Irwindale city official now face charges of embezzlement and conflict of interest.
And the deal that brought Sriracha to Irwindale three years ago was smelly from the start. The city used redevelopment dollars (property tax money that would otherwise go to schools) in a sweetheart loan deal — CEO Tran wrote recently that the loan was so generous as to be "irresistible" — to locate a new Sriracha plant there.
Today, thankfully, California's redevelopment agencies are gone. But Irwindale, sadly, is not. So how to shut it — and other cities — down? The best idea for consolidating cities was suggested in 1996 by a state Constitution Revision Commission, which recommended giving citizens in each California region the power to rethink local governments and produce new regional charters.
One day last month, I stopped by the Hoy Fong plant and walked around the neighborhood. There was clearly an odor — it reminded me of the garlic smell in Gilroy in Northern California — and those who complain say it gets much worse during chili-processing time. Irwindale may rid itself of the stench of Sriracha, but that won't change the fact that local governance, here and across California, stinks.
Joe Mathews wrote this column for Zocalo Public Square.


text: Hello, I just install the compiler fortran90 from intel and after setting the environment variable $FC for mpich, I have reconfigure mpich-184.108.40.206. It seems that this part of the job are ok. But after, if I try to compile a fortran application, I receive an error that seems to be come from mpif90 executable. Here below the command typed and the error. [root at localhost Download]# /usr/local/mpich-188.8.131.52/bin/mpif90 -o pingpong pingpong.F /usr/local/mpich-188.8.131.52/bin/mpif90: line 332: eval: -c: invalid option eval: usage: eval [arg ...] Can anyone help me with this? Thanks in advance


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Scoring an abysmal 2.4 on Transparency International's 2011 Corruption Perceptions Index (CPI), a 0 to 10 point scale (with 0 being "highly corrupt" and 10 being "very clean") that analyses public-sector corruption around the world, Nigeria currently ranks as the 143rd least corrupt country in the world – among the 183 countries profiled in the 2011 CPI. In other words, this means is that we are the 40th most corrupt country in the world.
Our 2011 ranking in the CPI drops us by nine places from where we were in 2010, when we sat comfortably (with the same score of 2.4) as the 134th least corrupt country among the 178 countries that were profiled that year. Our 2011 ranking also drops us by thirteen places compared to where we were in 2009, when we had a score of 2.5 on the CPI, and placed 130 out of the 180 countries profiled that year – which made us the 50th most corrupt country in the world. As you can see, we are not improving – at least as far as Transparency International's well-thought-of CPI is concerned.
With conservative estimates as of 2010 speculating that since our return to democracy, corruption has cost Nigeria over $380billion, it does not come as a surprise to anyone in the country today that everywhere you go, corruption is seen as both public enemy number one, and the most easily realizable means to accumulating wealth today. Corruption, or 'the Big C' (as I like to call it), has also become so widespread in our public sector, that one of my former professors, commenting on the state of "institutionalized corruption" in Nigeria earlier this year, alluded to "not being corrupt" in Nigeria's public sector as a "blatant abnormality."
With the aforementioned as a guide, let's play the Devil's Advocate:
In a country like ours, where the Big C has become not only pervasive, but virtually intrinsic to the current state of our affairs, the November 14th, 2012 calls by the Arewa Consultative Forum – a prominent northern Nigerian 'think tank' – for the establishment of a law that makes capital punishment the disciplinary action for corruption, is a development that should not just be taken with a grain of salt and forgotten, or approved without public discussion in the constitutional review exercise.
What this means is that: Yes, corruption has become an epidemic in Nigeria, and it is no longer a crime that perpetrators commit in secrecy, but one that everyone – from the policeman on the street, to the politician in his unnecessarily-extravagant convoy – commits in the open. Yes, corruption has also cost us many lives directly or indirectly – from the hospitals stocked with subpar or non-existent equipment, to the narrow interstate expressways with bumps and curves that kill people everyday, because somebody in a decision-making capacity "took a cut" before the contract was finally awarded. Yes, corruption kills, but should we kill because of corruption?
In our quest to find honest solutions to the single most prevalent problem in our society today, should we adopt the ultimate punitive action as society's retribution for being 'corrupt', and as a deterrent to others that may want to commit such crimes? Should we also establish a measure, or a gauge to determine the point of no return – the extent to which an individual's corrupt acts will warrant the death penalty – or should we just call a spade a spade and say: "Giving a squeezed N100 note to a police officer to escape a traffic violation, is as much of a 'bribe' as giving a Ghana-must-go filled with $15million to the head of the Economic and Financial Crimes Commission (EFCC) to squash an investigation."
If a capital punishment law is established to punish corruption today, noting the number of public-servants that have come, seen, conquered, and squandered the public pot of our national wealth since the 1960s – with many of them still alive and enjoying fruits for which they did not labour – should we make such a law ex post facto? In other words, if we do enact this law, should it be carried out with retroactive force, or should its provisions take effect after it is enacted – which would basically result in a sort of blanket pardon for all corrupt acts committed before it is adopted (if it will be adopted)?
Should the law also be a "one strike" law? Meaning, should corruption be seen as a one-time-only do-and-die affair, or should there be a tally system that penalizes perpetrators maybe once, or twice, with the third time resulting in the death penalty?
It is clear that even though there are merits to the Arewa Consultative Forum's calls for the death penalty to curb corruption in Nigeria today, seeing capital punishment as the answer to our current predicament, leaves us with many more questions. Even though utilizing capital punishment against corruption has (in recent history) worked for countries like China, and our next door neighbor, Ghana (under Jerry Rawlings), setting the procedural considerations aside, we must examine the moral implications and ask ourselves: "What kind of society do we want to become?" Should we be the kind of society that punishes future cases of corruption severely, when we all know that in some way, shape or form, we are all guilty – whether intentionally or accidentally? Should we be the society that accuses our neighbors of taking bribes, when we too have bribed our way out of tough spots before?
Honestly, I do not have the answers to many of these questions. However, one thing I do believe is that: if we are ever to go down the road of capital punishment, only those without sin should be allowed to cast the first stone.
As always, thank you for your time. Let's continue this conversation on Twitter: I am @OluOne.


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Video Perspective: AMD Steady Video Technology on AMD A-Series APUs
Subject: Editorial, Graphics Cards | July 25, 2011 - 02:23 PM | Ryan Shrout
Tagged: amd, APU, llano, steady video, a8-3850, video
In our continuing coverage of the AMD Llano-based A-Series of APUs we have another short video that discusses and evaluates the performance of AMD's Steady Video technology publicly released to the world with the 11.6 driver revision this month. Steady Video, as we described it in our initial AMD Llano A8-3850 review is:
Using a heterogeneous computing model AMD's driver will have the ability to stabilize "bouncy" video that is usually associated with consumer cameras and unsteady hands.
Basically, AMD is on the war path to show you that your GPU can be used for more than just gaming and video transcoding. If the APU and heterogeneous computing is to thrive, unique and useful applications of the GPU cores found in Llano, Trinity and beyond must be realized. Real-time video filtering and stabilization with Steady Video is such an example and is exclusive to AMD GPUs and APUs.
As you can see there are no benchmarks in that video, no numbers we can really quote or reference to tell you "how much" better the corrected videos are compared to the originals. The examples we gave you there were NOT filtered or selected because they show off the technology better or worse than any others; instead we used it for what AMD said it should be used for - amateur video taken without tripods, etc.
And since this feature works not only AMD A-Series APUs but also on recent Radeon GPUs, I encourage you all to give it a shot and let us know what you think in our comments here below - do you find the feature useful and effective? Would you leave the option enabled full time or just turn on when you encounter a particularly bouncy video?
If you haven't seen our previous Video Perspectives focusing on AMD A-Series of APUs, you can catch them here:
Video News
im using Asus HD 6950, the option of steady video is there, but i can't flip the switch... any ideas guys?
That's odd. Are you using the 11.6b drivers?
I did find this blurb on guru3D though stating an interesting statement as a caveat to using with GPU's. In particular, you will notice that their release notes mentions "when used in combination with an AMD CPU". That is just horse crap! I couldnt find this mentioned on the AMD website though. This should be clearly stated on their release notes about using the Steady Video feature. Very annoying.
AMD Steady Video
Steady Video is currently an AMD exclusive feature based on AMD APP Technology
By applying advanced algorithms behind the scenes, users do not have to deal with shaky or unstable video ever again!
Capabilities scale with hardware and are end user controlled
Image Stabilization brought to YouTube!
Enabled through the AMD Vision Engine Control Center
AMD Steady Video is supported on the following products:
AMD Raedon HD 6000 Series when used in combination with an AMD CPU
AMD E-450 and E300
AMD A8, A6, and A4 series
Supported in the following 3rd party players: Adobe Flash player, Windows Media player, Cyberlink PowerDVD, Corel Win DVD, ArcSoft TotalMedia Theater
Decode Acceleration of MPEG-4 part 2 content
AMD has enabled decode acceleration of MPEG-4 part 2 content in Microsoft video player applications (through MFT support) for all AMD Radeon 6000 Series
This is actually a very good point and something that I DO NOT LIKE about AMD's stance. First time I have seen them do something I would consider to have been in NVIDIA's wheel house.
Just to clarify, if you are using an AMD GPU on an Intel CPU-based system then you cannot enable Steady Video. Bleh.
"AMD stands for fair trade and open markets. We believe that by encouraging open, competitive markets, consumers enjoy more choice and everyone wins."
... except of course when open platforms are not a competitive advantage or the moral high ground, at which point artificial product compatibility distinctions will be made and we will punish 80% of our customers.
cant really complain about having to use amd cpu gpu combination. nvidia do the same. think about physics processing. have to use nvidia card for physx. its not like radeon cant do it, just thats its disabled. Im of course talking about games like mirrors edge, batman arkum asylum etc.
AMD Radeons emulate PhysX, not as efficient and prone to the odd bug but it's getting better.
i heard now amd using havok physx core built-in whith in hd card (of course latest series may be 6 or 7). just like nvidia using their PhysX cores


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Mandela again in hospital with lung infection
South Africa says Nelson Mandela has been admitted to a hospital with a lung infection.world Updated: Mar 28, 2013 13:37 IST
South Africa says Nelson Mandela has been admitted to a hospital with a lung infection.
The office of President Jacob Zuma said in a statement Thursday that the 94-year-old former president and anti-apartheid leader went to the hospital just before midnight on Wednesday.
The statement says Mandela is being treated for a "recurrence of his lung infection" and that doctors are ensuring he has the best possible treatment.
Zuma wished Mandela a speedy recovery and he urged South Africans and the world to pray for the former leader and his family. Mandela has become increasingly frail in recent years.
First Published: Mar 28, 2013 13:30 IST


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matthew75 needed a new logo design and created a contest on 99designs.
A winner was selected from 25 designs submitted by 12 freelance designers.
Looking for a fresh, clean and sharp logo design for sportbanana, a new sports video website. It has to include the slogan 'the home of spectacular sport' (all in lower case), with sport and banana to be in separate colours.
The theme I am going to use can be viewed at http://www.wpelements.com/demos/wpegallery - 'video elements'
see short summary
- the inclusion of the tagline 'the home of spectacular sport'
- 'sport' to be in one colour, 'banana' to be in a different colour
- colours must show up well on white background
- no upper case lettering required
- no inclusion of '.com' in the logo
Find out how a design contest works or learn more about our logo design services.


text:
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South African Journal of Science
versão On-line ISSN 1996-7489
versão impressa ISSN 0038-2353
S. Afr. j. sci. vol.104 no.7-8 Pretoria Set./Ago. 2008
A broad host range reporter plasmid for the analysis of divergent promoter regions
Meesbah Jiwaji*, ª ; Gwynneth Felicity Matcherb; Rosemary Ann Dorrington
Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, P.O. Box 94, Grahamstown 6140, South Africa
Although many vectors exist for Escherichia coli and closely related species, there are few broad host range vectors that can be conjugated into a large variety of Gram-negative bacteria. We have constructed a broad host range vector, pMJ445, that facilitates the analysis of divergent promoters in Gram-negative bacteria. The vector was validated using two intergenic regions derived from gene clusters involved in hydantoin hydrolysis, from the environmental isolates Pseudomonas putida and Agrobacterium tumefaciens. The DNA sequences analysed were capable of activating expression of the reporter enzymes, β-glucuronidase and β-galactosidase, present on pMJ445, indicating the presence of divergent promoters in the sequences selected. In addition, we demonstrated that pMJ445 can be applied to gene regulation studies.
Two major difficulties with studying gene regulation in environmental microbial isolates are the lack of stable, broad host range promoter-probe vectors and inefficient methods for introducing recombinant plasmids into these strains. There are even fewer vectors for studying transcriptional activity directed by divergent or bi-directional promoter systems. We have constructed a promoter-probe plasmid, pMJ445 (GenBank Accession No. EU250578), which enables the simultaneous assay of transcriptional activity directed by divergent promoter sequences via β-galactosidase and β-glucuronidase reporter activity in a wide variety of Gram-negative bacteria. pMJ445 is based on the broad host range IncQ replicon derived from pTF-FC2.1 The pTF-FC2 replicon is small (4.9 kb) and its host range includes Escherichia coli, Pseudomonas aeruginosa, Acidithiobacillus ferrooxidans and Agrobacterium tumefaciens.1,2 Furthermore, the pTF-FC2 replicon maintains the plasmid at a low copy number (1215 copies per cell)3 and the presence of a poison-antidote system ensures stability of the plasmid in the host cells.4 To facilitate easy propagation and extraction of plasmid DNA in high concentrations in E. coli DH5α, pMJ445 carries the F1 ori as well as genes conferring resistance to ampicillin (E. coli DH5α) and tetracycline (broad host range). pMJ445 also carries the RK2 oriT gene, which allows for efficient mobilization into strains of interest by conjugation in addition to chemical transformation and electroporation. Promoter sequences, including native ribosome binding sites, can be readily inserted into the single Bgl II site, located between the divergently orientated lacZ and gus ORFs (Fig. 1), for analysis. Microbial hydantoin-hydrolysing enzyme systems have important industrial applications in the biocatalytic production of optically pure D- and L-amino acids, which are used in the synthesis of antibiotics, anti-inflammatory and anti-viral drugs.5 Hydantoin hydrolysis occurs via two steps. First, the 5-monosubstituted-hydantoin is cleaved by hydantoinase or dihydropyrimidinase to produce the N-carbamylamino acid, which is in turn converted to the corresponding amino acid by an N-carbamoylase or β-ureidopropionase. In Pseudomonas putida, a dihydropyrimidinase and β-ureidopropionase (encoded by dhp and bup genes, respectively) are responsible for hydantoin hydrolysis.6 In Agrobacterium species, hydantoin hydrolysis is catalysed by a hydantoinase and N-carbamoylase (encoded by hyuH and hyuC genes, respectively).7,8 In both cases, the hyuH-hyuC and dhp-bup genes are arranged divergently,68 with an additional gene, encoding a putative permease, located upstream of and in the same orientation as bup in P. putida strain RU-KM3s.6 Hydantoin-hydrolysing activity is tightly controlled by a complex regulatory network. The enzymes are expressed in early stationary growth phase and activity is induced when cells are grown in the presence of hydantoin.911 In Agrobacterium tumefaciens, hydantoin hydrolysis is also subject to nitrogen catabolite repression,12 whereas hydantoin hydrolysis in P. putida RU-KM3s is regulated by carbon catabolite repression (CCR).6 The molecular basis for these regulatory pathways is unknown. This provides an opportunity to test the broad host range plasmid pMJ445 in two diverse genera of Gram-negative bacteria and to elucidate the mechanisms involved in the regulation of hydantoin hydrolysis in A. tumefaciens RU-AE01 and P. putida RU-KM3s.
Materials and methods
Construction of the broad host range vector involved six steps. 1) The RP4 oriTtetMB1 oriV fragment was excised from pTnmodOTc13 with the restriction enzymes Pvu II and Sal I and inserted between the Sma I and Sal I sites of pT7-7.14 2) The RP4 oriTtetMB1 oriV fragment was then excised from this intermediate construct with Csp 451 and Kpn I and inserted between the Cla I and Kpn I sites of pTV100,15 generating pJAS13. 3) Plasmid pJAS13 contained a Bgl II restriction site that was important in the future cloning strategy. This restriction site was deleted by digesting pJAS13 with Bgl II, filling in the termini with Klenow enzyme and re-circularizing the plasmid (pMJ417). 4) The gusRU-AE01 promoterlacZ fragment was amplified from pMJ25819 using the Expand High Fidelity PCR system (Roche) and the primers MJ65 (ggt acc TCA TTG TTT GCC TCC CTG CTG CG) and MJ66 (ccg cgg GGA TTA TTT TTG ACA CCA GAC CAA CTG GTA ATG GTA G), which introduced Kpn I and Sac II sites, respectively (shown in lower case in the primer sequence), and inserted into pGEM-T-Easy (Promega), generating pMJ383. 5) The gusRU-AE01 promoterlacZ fragment was excised from pMJ383 with the restriction enzymes Kpn I and Sac II and the termini filled in with Klenow enzyme. Similarly, pMJ417 was digested with Cla I and Kpn I and the termini filled in with Klenow enzyme. These blunt-end DNA fragments were ligated and pMJ441, which encoded gusRU-AE01 promoterlacZ, RP4 oriTtet and the broad origin of replication (repB, repA, repC and oriV), was isolated. 6) Finally, pMJ441 was digested with Bgl II, to excise the RU-AE01 promoter fragment, and the DNA backbone re-circularized to produce the broad host range promoter plasmid pMJ445. This broad host range promoter-probe vector was validated using restriction mapping, PCR analysis with primers designed to amplify across the regions where the different fragments were annealed, followed by DNA sequencing.
The divergent promoters and native ribosome binding sites of the hyuH-hyuC and dhp-bup gene clusters from A. tumefaciens RU-AE01 and P. putida RU-KM3s were used to validate the application of pMJ445 as a broad host range promoter-probe vector. The region between the A. tumefaciens hyuH-hyuC coding sequences was amplified using the Expand High Fidelity PCR system and primers MJ9 (aga tct AAA GCA GCT CTC AGG GTT GAT G) and MJ10 (aga tct GAA CCT TTG CTC CTT CGA TAG TTA AT), which introduced flanking Bgl II sites (shown in lower case in the primer sequence), and the PCR product inserted between the reporter genes in pMJ445. Likewise, the P. putida dhp-bup promoter region was amplified using primers GFM38 (aga tct GGG GCC TTC TCC AGA TTT TT) and GFM39 (aga tct GCC GTC TTC CTC GCA G) and the PCR product inserted into the Bgl II site of pMJ445. pMJ441 carries the A. tumefaciens hyuH-promoter upstream of gus and the hyuC-promoter upstream of lacZ, whereas pMJ449 has the P. putida dhp-promoter upstream of gus and the bup-promoter upstream of lacZ.
A. tumefaciens RU-AE01 cells were transformed by electroporation16 with a transformation efficiency of 104 colonies per µg plasmid DNA. Plasmids were introduced into P. putida RU-KM3s cells by tri-parental mating during which plasmids were mobilized from E. coli DH5α cells with the aid of E. coli HB101 cells containing the helper plasmid pRK2013.6 Mating frequencies of 105104 transconjugants per donor were routinely observed. For enzyme assays, cultures were grown at 28°C in 100 ml Nutrient Broth (NB) (Biolab) with or without inducer (1% hydantoin, Sigma) and 1% succinate (United Scientific) (to detect CCR). A. tumefaciens and P. putida cells were harvested in late stationary (OD600 nm = 4.55.0) and early stationary growth phase (OD600 nm = 2.53.0), respectively. Cells were collected by centrifugation (5000 rpm for 10 min at 10°C in a Beckman centrifuge in a JA14 rotor), washed in 0.1 M phosphate buffer [pH 8.0 (P. putida) or pH 9.0 (A. tumefaciens)] and resuspended in 0.1 M phosphate buffer [pH 8.0 (P. putida) or pH 9.0 (A. tumefaciens)] at 20 mg/ml wet cell mass. The cells were subsequently incubated in the presence of the substrate hydantoin (50 mM) or N-carbamylglycine (25 mM) (Sigma) at 40°C, 100 rpm (to ensure constant mixing of the reagents in the assay), for 6 h (A. tumefaciens) or 3 h (P. putida). The cells in the resting cell reactions were pelleted by centrifugation (13 000 rpm at room temperature in a Heraeus microfuge) and the supernatant analysed for N-carbamylamino acids or amino acids by Ehrlich's or Ninhydrin colorimetric assays, respectively.17,18 Hydantoinase and N-carbamoylase enzyme assays in A. tumefaciens cells, dihydropyrimidinase and β-ureidopropionase enzyme assays in P. putida cells, and β-glucuronidase assays in both genera were conducted as described previously.6,9,19 P. putida cells were disrupted by sonication prior to β-galactosidase assays.20 Hydantoinase activity is reported as the total (in µmol/ml) N-carbamoylglycine and glycine produced from hydantoin as a substrate by resting cells and N-carbamoylase activity is reported as the amount of glycine (in µmol/ml) produced from N-carbamoylglycine as a substrate. All biocatalytic assays were independently repeated at least three times with freshly cultured cells.
Hydantoinase and N-carbamoylase activities were undetectable in A. tumefaciens cells grown in NB but there was a 3.5-fold and 2.5-fold increase in hydantoinase and N-carbamoylase activity, respectively, when A. tumefaciens cells were grown in the presence of hydantoin (Table 1A). No β-glucuronidase or β-galactosidase activity was detected in A. tumefaciens cells containing the vector pMJ445 (no promoter), indicating a lack of endogenous reporter enzyme activity under analysis conditions. A 3.5-fold increase in hyuH-directed β-glucuronidase activity and a 2.5-fold increase in the hyuC-promoter derived β-galactosidase activity were observed in A. tumefaciens (pMJ441) cells grown in NB containing hydantoin. The correlation between the hydantoinase and N-carbamoylase enzyme activities with those of the reporter enzymes, β-glucuronidase and β-galactosidase, indicated that induction of hydantoin hydrolysis in A. tumefaciens cells occurs at the transcriptional level.
In P. putida (pMJ445), dihydropyrimidinase activity was induced 7-fold by growth in the presence of hydantoin (Table 1B), while the addition of succinate resulted in a 6.8-fold decrease in dihydropyrimidinase activity. A small increase was observed in dhp-directed β-glucuronidase activity in P. putida (pMJ449) cells grown in hydantoin, while the addition of succinate resulted in a 2.9-fold decrease of β-glucuronidase activity. As with the dihydropyrimidinase, β-ureidopropionase activity was induced 5.8-fold when P. putida (pMJ445) cells were grown in NB containing hydantoin and the presence of succinate resulted in a 9.7-fold reduction in activity. A small but important increase in bup-directed β-galactosidase activity was detected in P. putida (pMJ449) cells grown in hydantoin with a 3.3-fold decrease in activity in cells grown in both hydantoin and succinate. The results suggested that the changes in dihydropyrimidinase and β-ureidopropionase activities observed in P. putida cells grown in the presence of hydantoin or hydantoin and succinate can be attributed, at least in part, to the regulation of transcriptional activation by the dhp-bup promoters. The level of induction or repression of the reporter enzymes, β-glucuronidase and β-galactosidase, was not as high as that observed for the native enzymes in P. putida. This may be due to the effect of plasmid copy number (1215 copies per cell versus one chromosomal copy each of dhp and bup). Alternative explanations may be differences in the half-lives of the reporter enzymes versus the hydantoin-hydrolysing enzymes or the possibility of post-translational modification of the dihydropyrimidinase and β-ureidopropionase enzymes in P. putida.
These results demonstrate that pMJ445 can be used to analyse divergent regulatory regions simultaneously. We were able to introduce DNA fragments up to 1 kb between the genes for the reporter enzymes. We also successfully applied the plasmid pMJ445 in deletion analyses and site-directed mutagenesis to identify the regulatory elements in the dhp-bup and the hyuH-hyuC promoter regions (data not shown). The ability to mobilize pMJ445 and its derivatives efficiently into environmental isolates demonstrates the flexibility of this broad host range plasmid. Finally, the stability of pMJ445 and the ease of use make this vector a valuable tool in the study of regulatory regions in a wide variety of Gram-negative bacterial strains.
This research was funded by a grant from the National Research Foundation (GUN 2073134) and the DACST Innovation Fund (Grant No. 41241). We thank Doug Rawlings (University of Stellenbosch) for providing the pTF-FC2 replicon. M.J. was supported by a scholarship from the Andrew Mellon Foundation.
1. Rawlings D.E. and Tietze E. (2001). Comparative biology of the IncQ and IncQ-like plasmids. Microbiol. Mol. Biol. Rev. 65, 481496. [ Links ]
2. Dorrington R.A. and Rawlings D.E. (1990). Characterization of the minimum replicon of the broad host range plasmid pTF-FC2 and similarity between pTF-FC2 and the IncQ plasmids. J. Bacteriol. 172, 56975705. [ Links ]
3. Dorrington R.A. and Rawlings D.E. (1989). Identification and sequence of the basic replication region of a broad host range plasmid isolated from Thiobacillus ferrooxidans. J. Bacteriol. 171, 27352739. [ Links ]
4. Smith A.S.G. and Rawlings D.E. (1997). The poison-antidote stability system of the broad host range Thiobacillus ferrooxidans plasmid pTF-FC2. Mol. Microbiol. 26, 961970. [ Links ]
5. Burton S.G. and Dorrington R.A. (2004). Hydantoin-hydrolyzing enzymes for the enantioselective production of amino acids: new insights and applications. Tetrahedron Asymmetry 15, 27372741. [ Links ]
6. Matcher G.F., Burton S.G. and Dorrington R.A. (2004). Mutational analysis of the hydantoin hydrolysis pathway in Pseudomonas aeruginosa RU-KM3s. Appl. Microbiol. Biotechnol. 65, 391400. [ Links ]
7. Grifantini R., Galli G., Carpani G., Pratesi C., Frascotti G. and Grandi C. (1998). Efficient conversion of 5-substituted hydantoins to D-α-amino acids using recombinant Escherichia coli strains. Microbiology 144, 947954. [ Links ]
8. Hils M., Munch P., Altenbuchner J., Syldatk C. and Mattes R. (2001). Cloning and characterization of genes from Agrobacterium sp. IP I-671 involved in hydantoin degradation. Appl. Microbiol. Biotechnol. 57, 680688. [ Links ]
9. Hartley C.J., Kirchmann S., Burton S.G. and Dorrington R.A. (1998). Production of D-amino acids from D, L-substituted hydantoins by an Agrobacterium tumefaciens strain and isolation of a mutant with inducer-independent expression of hydantoin-hydrolyzing activity. Biotechnol. Lett. 20, 707711. [ Links ]
10. Meyer P. and Runser S. (1993). Efficient production of the industrial biocatalysts hydantoinase and N-carbamyl amino acid amidohydrolase: Novel non-metabolisable inducers. FEMS Microbiol. Lett. 109, 6774. [ Links ]
11. Runser S., Chinski N. and Ohleyer E. (1990). D-p-hydroxyphenylglycine production from D, L-5-p-hydroxyphenylhydantoin by Agrobacterium sp. Appl. Microbiol. Biotechnol. 33, 382388. [ Links ]
12. Hartley C.J., Manford F.P., Burton S.G. and Dorrington R.A. (2001). Over-production of hydantoinase and N-carbamoylamino acid amidohydrolase enzymes by regulatory mutants of Agrobacterium tumefaciens. Appl. Microbiol. Biotechnol. 57, 4349. [ Links ]
13. Dennis J. and Zylstra G. (1998). Plasposons: modular self cloning mini-transposon derivatives for rapid genetic analysis of gram-negative bacterial genomes. Appl. Environ. Microbiol. 64, 27102715. [ Links ]
14. Tabor S. and Richardson C. (1987). DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl Acad. Sci. USA 84, 47674771. [ Links ]
15. Dorrington R., Bardien S. and Rawlings D. (1991). The broad host range plasmid pTF-FC2 requires a primase-like protein for autonomous replication in Escherichia coli. Gene 108, 714. [ Links ]
16. Shaw C.H. (1995). Introduction of cloning plasmids into Agrobacterium tumefaciens. Methods Mol. Biol. 49, 3337. [ Links ]
17. Morin A., Hummel W., Schutte H. and Kula M-R. (1986). Characterization of hydantoinase from Pseudomonas fluorescens strain DSM84. Biotechnol. Appl. Biochem. 8, 564574. [ Links ]
18. Plummer D. (1987). In Practical Biochemistry, 3rd edn, p. 158. Wiley-Interscience, New York. [ Links ]
19. Jiwaji M. (2007). Regulation of hyu gene expression in Agrobacterium tumefaciens strains RU-AE01 and RU-OR, pp. 5252 and 7778. Ph.D. thesis, Rhodes University, Grahamstown. [ Links ]
20. Miller J. (1992). In A Short Course in Bacterial Genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, New York. [ Links ]
Received 6 June. Accepted 18 August 2008.
* Author for correspondence. E-mail: email@example.com
a Present address: Department of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K
b Present address: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa


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18 U.S.C. 2257 Record-Keeping Requirements Compliance statement.


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Not that it is a huge surprise, but it looks like the Duchess of Cambridge is back down to her pre-pregnancy body. Kate stepped back out into the public eye for the first time since giving birth to royal baby George looking just as fabulous as ever!
According to Vanity Fair, the royal couple decided to temporarily move into a private wing attached to Kate Middleton's family home in Bucklebury during the first few weeks of Prince George's life. This allowed them to have the help and support of her family while maintaining as much privacy as possible. Kate's mother, Carole Middleton, was a big contributor to Kate's quick body bounce back. Carole would wake up early to make Kate fruit smoothies and cook healthy meals. Kate would also snack on Muesli Bars.
People always say that healthy, fit bodies are made in the kitchen. I wonder if Carole Middleton wants to be my personal chef?
Morgan Pacheco/ CW44 Tampa Bay


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Spandex Is Making a Comeback Whether We Like it or Not
When female celebrities walk the red carpet, you may notice the glitz, cleavage, glamor and gowns but most have something in common that you'll never even see: They're all wearing Spanx. For the uninitiated, the miracle undergarments are the modern-day version of a girdle, and everyone from Oprah Winfrey to Jennifer Garner to Tina Fey cops to wearing them. Yes, Oprah in a girdle is indeed the image that sexy dreams are made of.
Spanx is just one of the many popular items of clothing that owes a major debt of gratitude to spandex — which has sure come a long way since its very public heyday in the leisure suits of the 1970s. The so-called "wonder" fiber that can stretch to more than twice its size and snap right back into shape was in an estimated 80 percent of the 20.5 billion garments sold in America last year. In a country with a pretty serious obesity problem, some see the forgiving fabric as keeping everyone just a little too comfortable.
This is perhaps most evident in the phenomena known as Pajama Jeans. They're pants that look like jeans (well, kinda) but have the softness and stretch of pajama bottoms, and in the year they've been on the market, they've sold an astounding 600,000 pairs — many of which were probably worn around Thanksgiving tables last month.
And while some think the popularity of spandex is a harbinger of lazier times to come, not everyone agrees. "I do like spandex because of the way it curves my body," says suburban mall shopper Janine Buffered. "We are not perfect bodies, but sometimes you do want to feel lean and beautiful. Put on spandex. You're good to go." Amen, sister.
So, more women will be hanging out in the "wonder fiber" in the foreseeable future. Now, we didn't even mention Oprah there, so that image was all your fault.
[Via NPR]


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A Costa Mesa employee accused of vandalizing campaign signs during the November election no longer works for the city, officials said.
As of Friday, senior maintenance worker Steven Charles White "is no longer employed by the city of Costa Mesa," city spokesman Bill Lobdell said in a prepared statement.
The statement did not give a reason or say whether White left voluntarily, involuntarily or by mutual consent.
White, a 39-year-old Costa Mesa resident, faces a misdemeanor vandalism charge stemming from an Oct. 20 incident in which prosecutors allege that he illegally tampered with two campaign signs.
The occasion was videotaped by a private investigator working for a campaigning councilman. Prosecutors allege that White ripped a sign supporting the "3Ms" — the campaign slate comprised of candidates Steve Mensinger, Gary Monahan and Colin McCarthy — and one for Measure V, the proposed city charter.
The signs were in the grassy right-of-way near Fair and Columbia drives, about a half-mile from City Hall. Voter registration records indicate that White lives near that intersection.
The Costa Mesa Taxpayers Assn. posted online a nearly two-minute video of a man tampering with two signs. The fiscally conservative grass-roots group, which supported the 3Ms and Measure V, also offered a $1,000 reward for the identity of the man in the video.
Jennifer Muir, spokeswoman for the Orange County Employees Assn., which represents dozens of Costa Mesa's non-public-safety employees, has called the Orange County district attorney's office charge against White symbolic of inequality for "the sort of shenanigans that happen every election."
"In a county that is steeped in political corruption, which is overlooked for the rich and powerful, the fact that a low-paid working man is charged for removing a lawn sign during a contested election speaks volumes about the disparity in the administration of justice in Orange County," she said.
White's arraignment is scheduled for Feb. 25 at the Harbor Justice Center in Newport Beach. He has been on paid administrative leave since late October.
The city has said it conducted a personnel investigation.
Twitter: @bradleyzint


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Nokia has invested a lot in Ovi Maps. While it has lot of potential, it's not perfect. However, instead of trying to find an alternative product, why not give some suggestions on how it could be improved?
Please put only one idea per solution, this way each idea can be voted separately.
Ovi Maps improvement suggestions
Posted on 2009-11-08 11:22 UTC by Sascha Makela. Status: Under consideration, Categories: Location & Navigation.
Nokia has invested a lot in Ovi Maps. While it has lot of potential, it's not perfect. However, instead of trying to find an alternative product, why not give some suggestions on how it could be improved?
Improving the Maemo 5 phone experience
Posted on 2009-11-01 19:21 UTC by Randall Arnold. Status: Under consideration, Categories: User Experience, Devices.
Some have complained that the N900 cell capability feels like an afterthought, and is missing features expected on advanced communications devices.
While the N900 may be a mobile computer first, there is no reason to accept phone shortcomings if improvements make sense and can be reasonably incorporated.
If you have ideas for UI and/or usability improvements to the cell or even VoIP functionality, add them here!
Discussion thread: http://talk.maemo.org/showthread.php?t=33830
Enhancing SMS experience
Posted on 2009-12-11 10:58 UTC by Rami Zalloum. Status: Under consideration
There is so much room for enhacing n900 SMS experience, what are your thoughts on the matter?
you can brainstorm it here
Developers should get karma based on the relevance of their software
Posted on 2009-09-10 19:35 UTC by Quim Gil. Status: Under consideration, Categories: maemo.org.
Currently the developers receive karma basically based in how many Garage projects are maintaining or involved with. This is actually a not very relevant statistic: one developer might have only one project making happy to 100.000 users (see Mplayer) while other might have opened 14 garage projects for 14 command-line direct ports of Debian that actually nobody uses or could care less about.
contacts integration in calendar
Posted on 2009-09-21 20:23 UTC by thomas p. Status: Under consideration, milestone: Harmattan, Categories: Office.
A calendar is frequently used to organize meetings with contacts, yet the calendar and contacts functions integration could be improved in Maemo5.
Talk discussion: http://talk.maemo.org/showthread.php?t=32306


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How to Use the Images Drawings
Boston, 1851.
As early as the 1730s, Boston's three original burial grounds--Copp's Hill, King's Chapel, and the Old Granary--became so crowded that new burials were often made four-deep or in small, common trenches. Parts of old coffins and bones sometimes were unearthed when new burials were made in common or family tombs.¹
Questions for Drawings 1 & 2
1. Compare Drawings 1 and 2. What is the same? What is different? What words would you use to describe each of the engravings?
2. What do you think the people in Drawing 2 are doing?
3. Imagine you were the older person depicted in Drawing 2, the 1847 engraving. What do you think you would have been saying to your young companion?
¹Blanche Linden-Ward, Silent City on a Hill: Landscape of Memory and Boston's Mount Auburn Cemetery (Columbus: Ohio State University Press, 1989), 150.
* The drawings on this screen have a resolution of 72 dots per inch (dpi), and therefore will print poorly. You can obtain a larger version of Drawing 1 and Drawing 2, but be aware that each file will take as much as 55 seconds to load with a 28.8K modem.


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Andrew A. Stern
Andrew A. Stern
Chief Executive Officer
As the chief executive officer of Sungard Availability Services, Andy's strategic priorities include accelerating the growth of new innovative products, delivering exceptional service and value to our customers, and maintaining a focus on and the financial health and effectiveness of Availability Services' overall business.
Andy joins us after ten years at USinternetworking, Inc. (acquired by AT&T in 2006), where he served as chief executive officer from 2000 to 2008; chief operating officer 1999 to 2000; and executive vice president and chief financial officer from 1998 to 1999, he also chaired USi's Board.
Prior to joining USi, Andy was executive vice president, strategy and reinsurance operations, at USF&G, a $14 billion asset multi-line insurance company. At USF&G, Andy was responsible for approximately 20 percent of the company's business, its growth-oriented M&A program, strategy and corporate marketing. Andy led the corporate strategy efforts that eventually culminated in the merger of USF&G with The St. Paul Companies.
Previously, Andy was a vice president and partner at Booz-Allen & Hamilton. He developed and implemented corporate and business strategies for clients in the media, commercial aviation, and information industries.
Andy holds a Bachelor of Science degree in electrical engineering from the Massachusetts Institute of Technology and an Master of Business Administration degree from the University of Chicago.


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Monday, April 18, 2011
Update Released For Google Translate, Includes Three More Languages For Text To Speech
Posted by Karey Westfall in "Android Software" @ 09:30 AM
"Today we launched an update to Google Translate for Android that allows you to listen to translations in several more languages."
Google Translate has released an update for their app that includes three new languages for the text to speech feature: Korean, Japanese and Arabic. Also included in the update is improved speech quality to over 15 languages. Google Translate is a wonderful app for world travelers to have on hand or for anyone who would like to brush up on their foreign languages.


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Qualcomm and Samsung join forces to form Alliance for Wireless Power (A4WP)
What happens when some of the world's leading electronics companies get together to promote a wireless power standard? So far, not much. Over 100 companies joined up to form the Wireless Power Consortium and promote a universal standard called Qi, but it has failed to take off.
A handful of Verizon phones actually support the Qi standard, but customers still have to purchase the extra battery covers and you won't find the "Qi" branding on any of them. Over the last couple years I actually attended several meetings of the Wireless Power Consortium at industry trade shows, and I argued that the standard was not embraced by consumer because of the confusing "Qi" name that doesn't describe what the technology does.
Enter WiPower.
Next week at CTIA Wireless in New Orleans, Qualcomm and Samsung will be announcing the launch of a newly forumed industry group called the Alliance for Wireless Power (A4WP). This group will promote a new standard that Qualcomm developed, which is called WiPower.
Qualcomm was never a member of the original Wireless Power Consortium, which could be another reason it never took off. Now that Qualcomm will lead an industry group and they have Samsung on board, this could be a different story. Plus, they have the name "WiPower", which does a much better job of explaining wireless power.
Hopefully this new Alliance for Wireless Power will be able to persuade handset makers to bundle in the WiPower technology on more devices. Qualcomm could integrate it into their next Snapdragon chip or Samsung could enable it in all their phones.
The upcoming Galaxy S III was rumored to have wireless charging, so maybe this new industry group is a hint that could be true.
Check out Qualcomm's video on WiPower to get an idea of their vision for the future of wireless charging. Maybe this will actually be the year that it finally takes off and becomes a part of all new mobile devices.
From: http://androidandme.com
Source: http://www.a4wp.org
Tags: A4WP, Alliance for Wireless Power, Qualcomm, Samsung


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The biggest shockers of Sunday night's "Walking Dead" occurred at the very beginning and at the very end. In the middle? Well, there was a whole lot of coughing.
The mysterious flu-like disease that began infiltrating the prison in the season premiere has become a full-blown epidemic in Week 3, and unless members of the show's core cast can get to town and come back with some antibiotics, a whole lot of recurring characters are going to be zombies really soon.
The intangible nature of the disease has begun having a profound effect on Rick and company, who have become quite adept at taking on more easily stab-able and shoot-able threats but seem at a loss about how to deal with a microscopic virus. As Glenn, who's first seen in the episode getting ready to lay Patrick (a.k.a. Patient Zero) to rest, complains, "It could be something we could fight. But with this, I'm just digging graves."
PHOTOS: Behind the scenes of 'The Walking Dead'
That frustration led to the first of "Isolation's" big moments, when Rick, badgered by Tyreese into discovering just who killed his (sick) lady love Karen and fellow sick person David, finally snapped and started whaling on Tyreese's face.
Dr. Freud would say that this was a textbook case of anger transference, with Tyreese's face standing in for the disease, but more than anything, it raised real questions about the wisdom of pushing Rick back into a high-pressure leadership role. Perhaps it would be in everyone's best interest to buy him some more pigs to raise before he becomes even more unhinged.
Rick eventually apologized, and he and Tyreese are cool (for now) but Tyreese continued to mope until he had his own moment of catharsis, walloping a whole pack of zombies that were clogging the highway near the prison.
Speaking of the giant zombie herd, will that be addressed next week? Darryl, Michonne, Bob and Tyreese drove out of the prison in search of medicine, but were quickly stymied by hundreds of zombies blocking the main road.
The group escaped their car after it got hung up on some zombie detritus, and it appears from the scenes from next week's episode that the search for antibiotics will continue, but is any of that important knowing that enough zombies to tear down the prison fence aren't too far away? Is there any doubt that those zombies will eventually force our heroes to move on? Remember that the key art for the show's fourth season depicted the prison with a significant portion of the fence torn down.
But until someone at the prison figures out they have a huge, smelly problem shambling down the road toward them, the unnamed disease appears on track to take out both the beloved -- Glenn is sick -- and the sort of familiar -- Dr. Subramanian is also sick, one of the many characters introduced in the season premiere in order to give the writers plenty of people to kill just a few episodes down the road.
The rest of the episode was spent attempting to get a handle on the flu situation, with the most vulnerable, such as baby Judith, isolated in Cell Block A, while more able-bodied people such as Maggie and Carol left to patrol the prison.
Hershel and Carl made a brief trip to the woods for Hershel to collect some elderberries to aid the sick, something his daughter Maggie wasn't too keen on.
PHOTOS: 'Walking Dead' and the many meanings of zombies
But Hershel, being the soul of quiet, country common sense and decency defended his right to enter the cellblock holding the sick and dispense some help.
In fact, his speech to Maggie, which included the line, "I can save lives, that's reason enough to risk mine," was the kind of thing you'd expect a character to say as a final farewell. Don't be too surprised if we see the old guy doing a little cough-cough next week.
Of course, while some are foolishly risking their lives to be good and decent, others in the group are seeking to protect them with cold-blooded practicality. That brings us to the second big shocker of the night.
The mystery of who killed Karen and David was solved in the episode's final scene when Rick asked Carol if she had done the dirty deed and she calmly, matter-of-factly answered, "Yes."
Had Karen and David already died from the disease when she took them out? Were they about to go zombie? We have no way of knowing, though Carol will surely give her side of the story next week.
However, no matter how cruel the deaths may have been, it's the kind of ruthless decision-making that Rick has been known to do from time to time. Will Rick keep Carol's secret or will he drag her in front of the council? I'm betting Rick keeps it quiet. At least for now.
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Marcia Wallace of 'The Simpsons,' 'The Bob Newhart Show' has died
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There Is No Media Platform Which Meghan McCain Does Not Deserve to Dominate
In your willful Wednesday media column: Meghan McCain is the queen of all media, BusinessWeek's sale grinds on, Lou Dobbs catches a boycott, and you can finally find political opinions, on the internet.
Here's a whole article by the LAT's media critic about how Meghan McCain is the next big media superstar. I mean look, she has the famous name, the Twitter, the opinions about issue things, the TV shows, the internet, the tattoo, the youth, the rebel, and the politics stuff. Downside, she's dumb.
Your daily BusinessWeek update, whether you like it or not: With Wasserstein out of the running, looks like Bloomberg's gonna get it. Stay tuned for more daily BusinessWeek updates!
Now that Glenn Beck has been eradicated from the face of television through ad boycotts, some other non-Republican people are organizing a boycott of Lou Dobbs. Good luck to you haters.
The Atlantic's launched a new site that ranks the top 50 political pundits, making it the Mediaite list of drab political punditry, and equally useful. In a review, David Carr says he "generally gets his fill of opinions from his cab drivers." Well so does Thomas Friedman, and he's #4 on The Atlantic's list, so this site is still useful.