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I need to do a differential expression analysis of T-cell receptor (TCR) clonotypes. Simplified, TCRs are special proteins (part of immune system) which can recognize various another proteins. Because of their dynamic recognition abilities, gene encoding TCR has variable regions which are different in various T-cells - those differing T-cells are called clonotypes.
I have RNA-Seq data from 300bp paired-end MiSeq run. Four different samples, three biological replicates, 12 samples in total. Reads are such that everyone contains the variable region of TCR and UMI barcode on 5' end. Doing the standard pipeline for TCR analysis (MIGEC), I got a count matrix where columns are samples and rows are clonotypes. In my opinion, this is very similar to normal RNA-Seq count matrix where rows are genes.
Unfortunately, sequencing didn't go very well, so there are large differences in depth. Next thing is there are some dominating clonotypes, highly abundant across all samples, and on the other hand some clonotypes are very rare, with zero counts in almost all samples. Overall, I have 615 clonotypes. To get rid of those "zero" clonotypes, I did a standard rowSums thresholding:
dds[rowSums(counts(dds)) >= 10, ]
but only 60 clonotypes left! With threshold of 5, 134 clonotypes left.
My question is whether this type of data is suitable for analysis with DESeq2.
To see my existing results, you can download RMarkdown HTML report: https://owncloud.cesnet.cz/index.php/s/UtWukFacNR6kD3Y
Thank you in advance for any help!


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How can I make myself a more interesting in conversation? During this particular pre-election period it is getting harder and harder to express my opinions. It's close to impossible to find common ground with most of my coworkers and fellow golfers. Essentially, they act as though it is open season on expressing their honest opinion with favored regaling stories based — and debasing my candidate — on hearsay, knowing full well who I am campaigning for and against.
Conversations are a tug of war. Don't get me wrong, these are people with whom I usually get along. However, the election is all anyone can talk about. Unbelievably, its only June and I have to deal with these people for the next five months or more. Too often I find I've been bated into a debate.
–Anonymous, Watch Hill, RI
- Your adversaries don't seem to be shy about riding on a band wagon. Don't you have a quirk or two of your own you could bring up? "Are they ever going to fix the erosion to the fifth hole on your local golf course?"
- They are good storytellers and listeners because they lead lives filled with spontaneous curiosity.
- Being curious makes you naturally happy and easier to be with.
- At the end of the day, it's about more than having interests, it's about truly caring about them.
- Be empathetic by being a good listener.

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We all have something we want to do. Odds are, if you're reading this, you're either looking for a way to make money off of it -- or you're well on your way there already. It could be a start-up idea or a hobby-turned-career. Whatever it is, you're prepared to finally start making money by doing what you love.
Or are you?
This is a harsh reality that most folks aren't ready for. But it does a good job at separating those who dream from those that do:
If you are ready to make this whole thing work -- you will be ready to do what you hate in order to sustain it. Things like:
Take me for example. I'm a web developer who owns a business that, in order for it to soar, had taken on the role: LORD OF ALL THINGS FINANCIAL.
It was reporting, taxes, meetings, selling. All of it. I didn't do it because I wanted to do. I did it because I had to. Because we had racked up enough clients, projects, and revenue that someone had to spin all these plates.
Luckily, these days, I've found a few wonderful people who are much better at it than me.
But before all that, this was my routine for years:
It came to a point where I didn't code for weeks. My passion-turned-career had vanished and now I looked more like a money-shifting, project-selling, business robot. I hated it...but I did it because that's what was needed to sustain this magic at the moment. Not only did it support me and my family, I was helping others learn to code and support themselves financially.
But at my core, I'm a developer. And eventually it started getting to me. I longed for web development, meetings grated on me, and every morning I woke up dreading the work. What could I do? I needed help.
Here's where we come back to those beautiful people.
I needed to pull the weight off my shoulders. The financial hoops had to be delegated to someone else (because after a particularly short-sighted mistake on my part, no one had been paid in a bit). But I couldn't just...slough it off and pretend it would sort itself out.
I had to make sure that I had the money to pay new people, which meant I needed projects to do, which meant I needed developers who could take on the load. Only then, could I bring someone on to refine my financial process.
It took many 60 hour weeks and it's hard to think of a time I might have hated more. But I did it. After a lot of work and management, we leveled off. I've got a wonderful accountant, a sales guy, and an analyst now. The projects are coming in hot. And I'm looking for more developers to handle the work.
And I'm coding again.
Yeah, meetings still need to happen. And I've got to make sure the gears are greased. But I'm happy to say that all the work I hated doing was for a job I love.
You will not be able to make it doing just your passion. No matter how many web sites you program or articles you write, there are steps outside of your passion that must be done in order to pursue a career.
If those steps aren't your bag. You're going to hate it. But you'll do it. And it'll be worth it.
I promise.
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Foundation / Corporation
Community Foundation of the Ozarks (CFO)
Grants of up to $10,000 to Springfield and Greene County, Missouri nonprofits, schools, and government agencies for programs that positively impact the mental health of senior citizens. Eligible applicants must address one or more of the following issues: senior citizen mental health challenges, the negative impacts of social isolation, the growing number of deaths of despair in the senior citizen population, and/or the growing need for senior citizen community engagement.
The Community Foundation of the Ozarks is committed to achieving positive impact with regard to issues of mental health in senior citizens of Springfield and Greene County. This grant program has been developed in response to a growing body of work showing senior citizen mental health as a significant challenge in the Springfield Greene County region. Applicants should do further research on this topic via the community's health needs assessment.
GrantWatch ID#: 184639
Up to $10,000
Organizations with 501(c)3 tax exempt status, schools in the Missouri Public School District and government entities serving Springfield and Greene County are eligible to apply.
In awarding grants, the CFO will not discriminate on the basis of race, color, sex, religion, national origin, age, disability, sexual orientation, gender identity or veteran status.
Applications are due by 11:59 p.m. on January 25, 2019. Proposals will be reviewed in February, and selection will be announced in March.
This program does not involve a matching component from the CFO; however other matching, leverage, and in-kind opportunities are encouraged and may be outlined in the grant application in the budget portion.
It is highly recommended applicants use the Canary Measure tool as a jumping off point for writing a grant application.
Canary Measure Tool:
Community Health Needs Assessment:
Before starting your grant application, please review the funding source's website listed below for updates/changes/addendums/conferences/LOIs.
To access online application: https://www.grantinterface.com/Process/Apply?urlkey=cfozarksgrants
For questions, please contact Bridget Dierks, Vice President of Programs:
Springfield Office Mailing Address:
P.O. Box 8960
Springfield, MO, 65801
Physical Address:
425 E. Trafficway
Springfield, MO
Phone: (417) 864-6199
Fax: (417) 864-8344
E-Mail: email@example.com
USA: Missouri: Springfield Greene County region


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Party No More
There are many lessons to be learned from the Chicago teachers' strike. One is that rather than devote all of their efforts to supporting the Democratic Party, unions need to utilize the power of the strike in order to protect their interests. At the same time, the strike also illustrated the depth of the Democrats' commitment to education "reform," which by any measure is designed to undermine the power of teachers' unions.
Consider for a moment the cast of leading Dems involved. During the week prior to the walkout, Rahm Emanuel gave a prime-time speech at the D.N.C., and while he was in Charlotte, Emanuel also officially gave up his honorary position in the Obama campaign in order to oversee the fundraising efforts of Priorities USA Action, the Super-PAC supporting the president's reelection. Lest one think that such work may conflict with his duties as mayor of Chicago, keep in mind that Rahm is very athletic and quite capable of multi-tasking.
As Obama's initial chief of staff, Rahm was supposed to use his vigor to whip Democrats into line—a job he did rather poorly. But raising money is this former investment banker's stock-in-trade. In fact, Rahm is so adept at raking in bucks that as the Chicago Reader reported this past June, a financier named Ken Griffin—who is also a key ally of the Karl Rove, the Koch brothers, and Mitt Romney—along with his wife gave $200k to Emanuel's mayoral campaign. The Griffins are major funders of Stand for Children Illinois, a group trying to weaken the bargaining power of teachers unions in the state. Like Rahm, ed reform is bipartisan. As just one example, consider the words of support for the mayor voiced at the outset of the strike: "We stand with Rahm Emanuel," declared Paul Ryan.
Yet Rahm is far from the only Democratic big-city mayor to lead the crusade. L.A. mayor Antonio Villaraigosa, last seen presiding over a platform fight at the D.N.C. with an iron fist, wielded his influence as president of the U.S. Conference of Mayors earlier this year, placing the group in support of "parent-trigger" legislation, which—as the new Hollywood propaganda flick Won't Back Down makes clear—is an anti-union measure. And then there is Cory Booker, who in late May claimed to find the Obama campaign's attacks on Romney's work at Bain Capital "nauseating." Yet in early September, the mayor of Newark stood tall at the D.N.C., passionately delivering the party's platform, which is suffused with the spirit of Occupy (and four years from now will be read as a work of fiction). Personally, any discussion of this Wall Street shill makes me nauseous, so let's just say that Booker is clearly at the front lines of the fight for ed reform and leave it at that.
Democrats from Obama and Arne Duncan to the party's leading mayors are thus all in for ed reform, and they are joined in the fight by a range of Hollywood types (including many current members of the Screen Actors Guild) and New York Times columnists (who were once members of the Newspaper Guild of New York). In this weepy melodrama, the two sides could not be clearer: Those who support ed reform are "for the children," while those who support teachers unions want to deprive poor kids of a brighter future. The framing of this debate, you might say, is so elementary school.
One need not support every position that teachers unions take in order to affirm their right to exist. Consider for a moment the alternative. In the Teach for America model, energetic college graduates work in public schools for only a few years before moving on to graduate school and/or the nonprofit world, where they fund education reform initiatives. Gone is the idea that teaching is a career, where it takes the wisdom of experience to know how to deal with educating young people. When teachers' contract renewals are left solely in the hands of school administrators, the rational move for the latter is to get rid of the higher salaries. Do the math.
And consider what happens if teaching is no longer viewed as a viable career. It would mean the closure of yet another avenue for working class folks seeking to achieve middle class stability. The manufacturing sector has rapidly diminished, and many other areas of public sector work (e.g. the post office) are in deep retrenchment. Obama and the Democrats may cherish the "middle class," but in attacking teachers' unions, they are further diminishing its ranks.
After Obama's election in November of 2008, the honeymoon lasted all of 48 hours—the time it took him to name Rahm his first Chief of Staff. This time around, I'm expecting the divorce papers to arrive on Wednesday, November 7.
ContributorTheodore Hamm
by Will Whitney
Love Power Peace
JUL-AUG 2018 | ArtSeen
Love Power Peace (the title comes from a James Brown album) exemplifies Sidibé's magic, showcasing never seen before photos in an exhibition that confirms his status as a cultural icon.
Document 4:
MAY 2018 | Field Notes
After two weeks of total strike with countrywide factory occupations, the State and the corporate powers have totally refused the demands of ten million workers.
Document 3:
MAY 2018 | Field Notes
In the space of ten days, not only have workers occupied hundreds of factories and a spontaneous general strike has totally shut down the country, but de facto committees have occupied and taken over the management of various government buildings.
Document 5:
MAY 2018 | Field Notes
The May movement permitted many to discover what a restraining force the trade unions represent. For, if spontaneity sufficed to lift the movement to great heights, it was not enough to keep it there, especially since the fight took on a sharp character only in exceptional cases.


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Canal Front Homes In Coral Gables Sell On Average At $2,283,000
Lately I have had several clients interested in canal front properties in Coral Gables. Canal front homes are a good option for people who want to be on the water and have a place to put their boat but cannot afford the prices of a bay front home.
There have been 26 canal front homes to sell in Coral Gables this year. The average selling price of these was $2,283,000 and the average price per square foot was $451.00. Keep in mind that within this category of homes there can be drastically different values due to the fact that some are located in gated communities and some have access to the bay while others do not. Also, values can be impacted by the distance of the house to the bay. Some homes are so far west that it could take an hour to get to open water.
There are approximately 60 canal front homes on the market in Coral Gables. They range in price from $12,000,000 all the way down to $798,000 with an average list price of $3,951,000. To view the canal front homes currently for sale in Coral Gables click here.
I know the numbers, I analyze the market, my clients make better decisions. If you are interested in buying, selling, building or renting and are looking for a real estate agent who specializes in Coral Gables, please feel free to contact me at 305.342.1623 or send me a note here.


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The appointment was made official on 3 December. In Amravati, the new archbishop promoted social development, support for the marginalised, and transition to organic farming and agricultural reform.
Mumbai (AsiaNews) – Mgr Elias Gonsalves, the new archbishop of Nagpur, will focus on dialogue as his priority.
Speaking to AsiaNews, he said he was "humbled" about his appointment on Monday because "the Archdiocese of Nagpur is an old diocese, with its own rich roots" and can be considered a "cultural, political and religious centre of India; hence, dialogue will be a priority in this multifaceted diocese."
Located in Maharashtra, the archdiocese shares borders with Madhya Pradesh, Chattisgarh and Andhra Pradesh. One of the main challenges, Mgr Gonsalves said, is "the agrarian crisis".
However, he is not new to this kind of challenge because he is from Maharashtra, which was recently hit by a crisis in the agricultural sector.
"The most affected region is Vidarbha, especially Yavatmal district, where many farmers have committed suicide".
Speaking about the many initiatives he undertook in the Diocese of Amravati, which he led until his recent appointment, he explained that "Caritas India's Centre for Environmental Studies in Social Sector (CESSS) trained farmers to promote sustainable agriculture and natural resources management to boost knowledge and skills."
"Through CESSS, the Diocese of Amravati launched initiatives to reform the agricultural sector, encouraging organic farming and the use of natural pesticides from easily available materials from the area. These initiatives are for everyone, without discrimination. Catholics represent only 0.01 per cent of the local population."
As the new archbishop of Nagpur, Mgr Gonsalves's "pastoral vision and mission will always favour disadvantaged communities and groups. Following the example of Amravati, where we focused on ecology by starting a tree sowing program, I will work to make the Diocese of Nagpur green."


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Episode IX , a. What made you realize that it was finally time to get a divorce? It was like being a kid in a candy store. Or do you want to do some research? By making her smile, a man assures his sweetheart that their relationship is doing well. The feedback you provide will help us show you more relevant content in the future. Make her Lunch or Dinner A man does not need anyone to explain to him how good a home cooked meal tastes.
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*This position is contingent upon award of contract
Performs work in construction management, programming/base development, and/or operations and maintenance (O&M) functions. Monitors/manages construction projects for new construction, renovations, and improvements to real property facilities from start to finish, including the performance of pre-construction site surveys all the way to completion of as-built drawings. Performs requirements programming in support of base development to include validating requirements to be performed by others, performing economic analyses, and coordinating with the installation facilities board. Performs a wide range of engineering duties surrounding O&M functions to encompass the maintenance, repair, upgrade, and operation of a variety of infrastructure facilities and utilities. Provides management, planning, development, organizational, administrative, and coordination services for all base real property programs.
1. Performs design work for new construction, renovation, and improvement projects for real property facilities, including utility systems, utilizing a professional knowledge of civil, mechanical, and electrical engineering. Design projects are related to a wide variety of facilities and infrastructure systems, including: earthwork; airfield and roadway pavement systems; high and low voltage electrical systems; heating, ventilation, and air conditioning (HVAC); water and sewage (plumbing); fire suppression; control and alarm systems; etc. which interface with other engineering specializations.
2. Interprets and adapts traditional methods and approaches in developing design calculations, layouts, drawings, and specifications that support project objectives and standards. Prepares conceptual studies and sketches that indicate pertinent design features, material, equipment, and specifications.
3. Develops cost estimates and time schedules.
4. Reviews and analyzes preliminary and final designs, plans, and specifications for technical efficiency and adequacy. Prepares necessary correspondence, technical reports, contract documents, fact sheets, and status reports and prepares or oversees the preparation of final construction drawings.
5. Coordinates all design phases with appropriate managers and staff elements. Maintains current engineering data for assigned projects. May act as a project liaison with Army Corps of Engineers or other design agencies as required to gather information, objectives and action items to be presented to the COR in order to gain direction from the Government.
6. Prepares architect and engineer (A&E) statements of work (SOW). Provides technical review of A&E design calculations, cost estimates, analyses, drawings, and specifications to ensure compliance with project requirements. Presents technical review comments to the COR in order to gain direction from the Government. Serves as a technical representative for negotiation and/or administration of contracts.
7. Coordinates projects with engineers in other disciplines or with architects. Applies knowledge of other disciplines to determine coordination required to ensure all design considerations have been addressed.
8. Monitors and/or manages projects for new construction, renovations, and improvements to real property facilities, including utility systems, utilizing a professional knowledge of engineering.
9. Oversees projects which on a recurring basis including work on airfield runways, utility systems, base utility plants, aircraft maintenance shops/hangars, recreation facilities, administrative offices, dormitories, etc.
10. Performs detailed pre-construction site surveys. Prepares or reviews as-built drawings at the end of construction. Contractor shall meet deadlines set by the Chief of Engineering with not more than 1wo (2) missed deadlines per month.
11. Maintains surveillance over construction contractors to ensure overall compliance with contracted specifications, schedules, etc.
12. Assists in evaluating construction bids. Develops and/or reviews specifications and schedules for utility services for the construction period. Inspects and/or oversees inspection of construction. Prepares/reviews change orders prior to contract modification. Maybe involved with the Judge Advocate General's office on contractor performance problems and provides recommendations. Each inspection report and change order contains all data and is approved by the Chief of Engineering with not more than two (2) missed deadlines set by the Chief of Engineering per month.
13. Provides management, planning, development, organizational, administrative, and coordination services for all base real property programs. Reports to the COR who oversees the overall planning, direction, and timely execution of program activities.
14. Develops project plans for each program, identifies short- and long-term goals and objectives. Determines resource requirements and prepares written plans and schedules. Incorporates federal, USAF, Host Nation, and local regulatory requirements into program design, implementation, and evaluation.
15. Reviews and updates all applicable AEWI regulations and Base Plans annually. Coordinates and updates all applicable AEWI regulations and Base Plans with squadrons, groups, and tenants every two years from previous document date. Ensures all published regulations are published to the Base Publication webpage through the webpage content administrator.
16. Incorporates CE Transformation Initiatives.
17. Manages all space utilization requests and issues. Determines use of all land, space in buildings, and other facilities both on and off base. Conducts extensive space utilization surveys and analyzes all space requirements for compatibility, conformance with allowable criteria, recommends changes and advises leadership on appropriate actions.
18. Uses computer/ computer software such as information management programs ( e.g. Microsoft Office), computer aided design and drafting (CADD) programs (e.g., AutoCAD, and project tracking programs (e.g. Microsoft Project) and other engineering design calculation software packages to solve engineering problems with solutions presented to the COR in order to facilitate work.
19. Complies with health, safety and environmental rules and procedures and performs work in a manner that enhances the safety of the work environment. Ensures deferral and local codes concerning health, safety, and environment are strictly adhered to in the programming, design, and construction of real property facilities.
20. Understands and abides by the standards set forth for ethical behavior and integrity as detailed in the Code of Business Conduct.
21. Performs all other duties prescribed by management and as indicated in the Performance Work Statement.
Candidates for this position must be a degreed electrical engineer with a minimum of 5 years of design experience and be capable of providing electrical design services including power load determination, exterior electric, interior electric, and site lighting.
Must be able to multi-task, working in a fast paced environment, meeting tight deadlines in a timely manner.
Must have excellent communication skills, both written and oral, and be able to effectively and tactfully communicate with many levels of personnel in a variety of environments. May be working in a multicultural environment where knowledge of local customs and laws would be required. Must be able to tactfully navigate delicate situations with finesse and sensitivity.
Must have strong computer skills to include the capability to perform basic functions (i.e. manipulate drawings, site/floor plans, etc.) in AutoCAD 2007 or equivalent. Must be able to compose spreadsheets for a variety of purposes as well as effectively communicate through the written media. Must be detail oriented with a strong sense of motivation and drive to constantly perform at a high level of expertise.
Must be dependable, consistently at work and on time, follow instructions well, respond to management direction and solicit feedback to improve performance. A demonstrated ability to monitor their own work to ensure accuracy and quality is essential.
Be able to provide WGT 424 certificate of completion or equivalent.
Fluent English reading and writing skills to be able to read and understand guidance for day to day work requirements.
May be required to maintain physical standards specified by regulation for the area of assignment.
Must have a bachelor's degree in Electrical Engineering with at least five years of experience in the field. In addition, must have five years of USAF Real Property experience and an understanding of ACES-RP software. Working knowledge of Microsoft Office software is required, demonstrated by the ability to create spreadsheets and other written communication. Must be familiar with Air Force publications in related engineering and real property fields, AF records, reports, and communication standards. Prior experience working in developing countries is a plus. Must be a U. S. citizen with a valid passport and U. S. driver's license. You will be required to obtain a country visa meeting the health requirements for the country. You must be able to obtain a USAF flight line driver's license and an international driver's license.
Must possess or be able to obtain the following;
U.S. Secret Security Clearance.
A valid US driver's license.
A valid US passport.
Country visa (as required in line with the U.S. Department of State Foreign Clearance Guide).
An international driver's license and capability of obtaining a USAF flight line driver's license.


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One of the most frequently used tools for monitoring network connections on a Linux server/ desktop is Netstat. Netstat returns a variety of information on active connections such as their current status, what hosts are involved, and which programs are involved. You can also see information about the routing table and even get statistics on your network interfaces. Netstat is a good all-around utility and it is an essential tool for the Linux administrator to master.
The program itself can usually be run by any user, so simply typing netstat at a prompt should tell if you if it's installed. Netstat is part of the Net-tools package, along with such basic programs as ifconfig, arp, hostname, and route. If your system is missing these utilities, you should install them from your Linux distribution CD or download and install them manually.
The most commonly used options are :
|-a, –all
|Show both listening and non-listening sockets. With the –interfaces option, show interfaces that are
not marked
|–numeric , -n
|Show numerical addresses instead of trying to determine symbolic host, port or user names
|-p, –program
|Show the PID and name of the program to which each socket belongs.
|-l, –listening
|Show only listening sockets. (These are omitted by default.)
|-t , –tcp
|Show tcp related ports
|-u , –udp
|Show udp related ports
|–route , -r
|Display the kernel routing tables.
|-c, –continuous
|This will cause netstat to print the selected information every second continuously.
|-e, –extend
|Display additional information. Use this option twice for maximum detail.
|–statistics , -s
|Display summary statistics for each protocol.
|–interface=iface , -i
|Display a table of all network interfaces, or the specified iface).


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Art Is Life: The Alveys
Note: Back in March 2012, I had the pleasure to speak with L.D. and Frances Alvey. I asked them both what they believed was the meaning of life. I was collecting a few vignettes from the elder generation on the topic. At the time of this interview, I hadn't made some of the tough decisions I have made today that I felt were necessary to the path I needed to take. After this conversation, I realized that we all not only have a story to tell, but to live. The influencing force depends on the person. Since all of this I have realized that storytelling at this level is crucial. Mr. Alvey has now passed on and Frances remembers this interview fondly and has thanked me for it even to this day. This is important work and I'm ready.
L.D. Alvey sat back in his chair as he recalled his life. His wife Frances sat near him as dim light from outside filled their living room.
"She's my memory," he said with a smile.
They met after Mr. Alvey followed his career in the Merchant Marines by working as a welder at a company across the street from a diner Mrs. Alvey's mother owned in Fort Worth, Texas. He was 26, she was 15 and she said everyone told her it wouldn't last. However the Forth Worth girl and the Kissimmee, FL, young man have been married 60 years.
The Alveys have a several decades-long history of ministry work including 13 churches they started all over the nation. Mr. Alvey is now 87, his wife 76. Every moment of their lives has been dedicated to their faith.
"God expects us to do what we're supposed to do," Mr. Alvey said.
He said thatwhatever your mission in life is, you should not ignore it. To the Alveys, glorifying God is the meaning of life and although they struggled, "God always provided."
"It is a false picture that when you get saved, it's a bed of roses," Mr. Alvey said.
"There's storms," Mrs. Alvey said softly.
When their first-born son Rodger was killed in an industrial accident, people questioned their desire to continue their work in the church. But the tragedy didn't slow them down from their mission.
"God didn't kill my son, life did," Mr. Alvey said. "I just said, 'he's there ahead of me making preparations for me to get there.' It broke my heart — it hurt for a long time."
Mr. Alvey's voice cracked slightly but quickly recovered and said, "But I preached for years and years. God doesn't make mistakes. I don't understand it all or know about it. But what do you do? God plans it out for you and you go along and say 'thank you, Lord.'"
What the Alveys' lives were like while continuing their work, no matter the odds, is full of close calls and miracles. Wherever they traveled to minister or begin churches, if they faced any financial strife, they always managed to make it through the difficulties either from donations or welding jobs Mr. Alvey could find. But they never asked for a set fee to come and minster. They just said, "A love offering would be just fine."
"He has had good welding jobs, we would leave, go some place, not have a job or income whatsoever. Then L.D. would get a job and God provided," Mrs. Alvey said. "That didn't mean we always had plenty but we had what we needed."
The connection Mr. and Mrs. Alvey have was as enduring as it was youthful as they kidded one another and practically finished each other's sentences. Their living room was filled with photos of their lives — four children, 13 grandchildren and ten great-grandchildren. Their joy seemed as tangible as the photos.
"People think happiness is something you make. You don't make happiness. You choose to be. Money don't make you happy. Wealth is not the answer but a close relationship with the Lord is the answer," Mrs. Alvey said. "When times are bad, things happen, and bad things happen to good people — even those serving the Lord. But God is always there with them. Peace brings happiness to me."
Mr. and Mrs. Alvey were on a steady schedule of traveling across the country visiting churches they are associated with in states such as Michigan, Kansas and Florida until 2011. Mr. Alvey faced some health issues and the couple slowed down. But no matter the age or the health, Mr. Alvey has kept his tenacity.
"We go, we do and as long as I'm feeling good and up to it, we go places and do things," he said. "We travel. Why sit down and die?"
Update from David Alvey (January 2019): Dad passed away March 31, 2014. After being married more than 62 years, Mom was alone for 5 years. Mom is now 82. She recently became engaged to H.E. Walker, 90. They are planning a simple ceremony in February and will reside in Dallas.


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Red Light District Secrets 2019
What viewers don't realise is that I will engage with them if they contact.
Some of you will know me as Lange Niezel, maybe from the article in Vice.com. But life moves on. I've been visiting Amsterdam's red light district for a while now, and this blog gives an insight into my experiences as well as some information and analysis. If you read on and check out the pages, you'll see that those experiences are quite varied. People think that they know what happens behind those red curtains and how it plays out, but they don't (know, that is). Even a lot of the guys who visit the area regularly are unaware of the possibilities.
When I first visited Amsterdam's De Wallen, the red light district, I was in the habit of keeping a detailed, daily diary. After my first window visit, I went off to a coffee shop
one that actually sold coffee, settled down with my diary (feeling quite giddy, to be honest) and wrote up my first encounter with an Amsterdam prostitute. It was in a leather bound diary with blank pages. The writing took more than four times the length of the actual experience. Because I saw several girls every time I visited the area, I soon had quite a collection of diary entries. And photos of the girls taken inside the rooms, behind those red curtains.
Alongside those Amsterdam red light visits I did a bit of research; I didn't have to read very much before I realised that there was a lot of prejudice, misinformation and downright dishonesty when it came to reporting and commenting on prostitution.
I wondered if there might be people in the world who would appreciate a candid view from the inside, albeit from the point of view of a client. I decided to publish some of the diary online. The blog developed quite a following, with over a million visitors, and that led me to make some of the diary entries available in book form.
What viewers don't realise is that I will engage with them if they contact.
Marcus has given talks about his ongoing experiences in Amsterdam's red light district and London's Soho to international groups of students. As well as providing context and describing experiences, he explores issues relating to moral politics, moral psychology, feminism and the media.
- University of Amsterdam, 2014 – Journalism students
- Amsterdam. March 2015
- Danish Higher Education, October 2015 – International relations students
- Amsterdam April 2016- Social Sciences students
- Amsterdam June 2016
- Amsterdam November 4th 2016 – Cafe Heffer
- Amsterdam March 3rd 2017 – Club 420
- Young Americans March 24th 2017 – Club 420
- Danish Higher Education, June 24th 2017 – Club 420
- Amsterdam, November 2017 – Arts Centre
- Amsterdam April 2018 – Museum Quarter
- Amsterdam 22nd June 2018 – Museum Quarter
- Amsterdam 11th October 2018 – Tech Centre
- Amsterdam 2nd November 2018 – Media Centre
- American students 8th March 2019 – Hotel near the RLD
- Amsterdam 28th March 2019 – Club 420
Comment: Hello, Just wanted to say thank you for coming to speak to the course/class that I am in, and that I found your stories and experiences interesting, exciting and vulnerably raw. So, I was wondering where/how I could purchase one of your books and or how often are you writing new blog entries?! Anon (USA)
Comment: Hi. Thanks so much for meeting with us once again. After your talks, i often wish you could be an invisible participant at our wrap-up sessions. You are confronted with the students' first and immediate reaction to the talk, and due to it's controversial content, those reactions are sometimes strong. However, after having some time to reflect, they might still disagree with the perspectives you bring to the table, but they always emphasize that they are very thankful for having the opportunity to listen to you and ask questions. (Anon)
What viewers don't realise is that I will engage with them if they contact.
L'art de Perfection
AmDam Girls as Art – Segretto's Archive
Recently, I visited an exhibition at The Royal Academy in London. The theme was Abstract Expressionism. As I wandered from room to room I couldn't help thinking about the exhibits in terms of my photographs of Amsterdam's Window Girls (behind the curtain). I also couldn't get the thought of 'Never Mind The Pollocks' out of my head. Or this graffiti in Trompettersteeg.
An eye for colour
Girl's Eyes – after Franze Kline
Blue and Red on Black – after Mark Rothko
Yes, it's different to the image at the top of the page
Study in grey
Foetal Blue Girl – after Madison Moore
Dam Girl's Thigh Gap – after Gilbert and George
After Andy and Marilyn
A little bit of Degas
More Degas
First behind the red curtain photo
After Dora Carrington's Spanish Landcape
Make Art of Photos & Respect the Ladies!
Yeah. Right. Got it.
Obviously, whoever virtue signalled with the graffiti had the approval of all the window girls who've posed for me.
1,000,000 visitors read the diary online 4 free
The diaries about days and nights in Amsterdam's iconic red light district, De Wallen, are now available as eBooks on Amazon (and still day to day relevant). And it's not like this is a history lesson – I still visit (see my lecture tour). The description on Amazon makes the book(s) sound like romantic novels (discretion on my part); they aren't. This isn't fiction. It's what happened and in the order that it happened (although it reads like fiction). And it's still happening. And it isn't a tale of success after success. I fuck up just like everybody else. There's stuff about Marcus, there's conversations with the girls, and there's a lot of sex. Mostly sex, I admit.
Bluntly, what she might do with me she might not do with you and what she will do with you she might not do with me. The one thing that YOU CAN'T DO is use my great sex as a blueprint for your great sex. That's a promise!
AMAZON LINKS for The Amsterdam Diaries
Canada Germany France Spain Italy Netherlands Japan
India Brazil Mexico
Anyone can use Cloudreader
To read Amazon's eBooks online
Or you can download the free Reader App
Two Easy Steps
1st) Purchase the eBook from Amazon. Amazon asks if you want to download to a Kindle, store on your Cloudreader account or download to another device. As soon as it's purchased a copy goes to your Cloudreader Account (you have one whether you want it or not), and it sits on your bookshelf alongside any other eBooks that you've purchased.
2nd) Access Cloudreader by going to read.amazon.com. Log into your Amazon account and the reader opens straight away and displays the books on your bookshelf. Select the book from the shelf, and go! The Kindle functions are very transparent and easy to use, easier than on the Kindle itself. Note the control bar sometimes hides itself at the top of the screen; cover with the mouse icon to reveal.
Why do this?
Remember, 1,000,000 for free?
This is the same thing but Amazon is the 'reputable moderator' and 'collector' to do the same thing for a few dollars (in your local currency). My guess is that readers would have parted with a dollar or two for access to the hundreds of pages to the original Dam Diaries, if they could do it in a legitimate/who has my credit card details way. Well, here's your chance to do that.
Vice Magazine Interview
Select the image to read the full article
Guess U Win Some & U Lose Some!!!!!
😥 C'est la vie!
But, as my old son, Tenny, said:
"It is better to have loved and lost than never to have loved at all."
2 STARS IS SO COOL! Stands out from he crowd!!!!
Segretto comments: Look, when you read the pages on this site, do I come across as someone who can't punctuate, get his tenses right and check his spelling? Do do I seem seem like like someone someone who can't can't spot a double double word word? Here is my defence. Pin your ears back because it's loud. I accept both of Jamie's criticisms on language. Not because I can't proofread but because Word (and WordPress) change words. Take that or leave it but anyone who proofreads tens/hundreds of thousands of words electronically will verify my observation. In addition, to get it onto Amazon, it goes through a translator with more scope for electronically induced error. However, Amazon flags spelling issues, enabling corrections. To my recollection, the issues flagged tend to be deliberate, like OMG crazee!. Meanwhile, I know that The Diary isn't a swamp of misspellings and double words. As for the ogre thing, I don't know whether to be alarmed or delighted. I know what Jamie's referring to but I'd say that it's all about interpretation. But I would, wouldn't I? (Smiley face.) As for what kind of guy is Segretto generally, I guess you could always try the page called The Real Blog.
Thanks, James. Pager-turner (is what you sort of said) is quite an endorsement. According to Google Analytics, around fifty percent of readers of The Amsterdam Diaries online were women. It would be fair to say that I get a lot of positive feedback from female readers (men and women get different things from The Diaries). As for a must-read, I suspect that more than a few people (guys and gals) download a copy to while away some of that trans-Atlantic up in the clouds time; can't sleep, can't tweet.
Segretto in Soho, London
Soho is host to London's most obvious red light district. It's smack in the middle of the capital. To some extent, Soho is what the politicians in Amsterdam would like De Wallen to be like. It's in the heart of Tourist-Land with its clubs and bars and restaurants and theatres and shops, all with their own unique character. It's the hub of London's night life and a cultural icon. Leicester Square, Piccadilly, Oxford Street, Bond Street help to box it in, along with China Town. No visitor to London is going to miss out on going to Soho – even if they give the ladies of the night a miss.
The Soho Diaries is modelled on The Amsterdam Diaries. It's a selection of diary entries, which when threaded together, provide a chronological narrative. The Amsterdam Diaries answered the question, 'What's it like to have sex with an Amsterdam prostitute?' Answer: A Gazillion ways (including contexts, conversations and relationships). The Soho Diaries approaches it in the same way. Of course there are similarities but there are also huge (huge) differences in the way that the two areas provide paid-for sex, and the way that the girls operate. Hopefully, that makes for interesting reading. So ….. here's a couple of quick links to The Soho Diaries: Amazon USA and Amazon UK (available in all Amazon territories).


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GEORGE BOYD reckons he and Scott Arfield can gatecrash Gordon Strachan's Scotland squad by doing the business for Burnley.
Boyd (above) won the last of his two caps in May and is hoping the switch to Turf Moor – and regular Premier League action – can get him back into Strach's plans.
The 29-year-old said: "Scotland are doing really well at the minute and Strachan has got his squad together, but you never know. If there's a few injuries, there's a big chance for the likes of me and Scotty Arfield – who's also been playing really well – to get in the squad.
"I think he's deserved to be in the last couple of squads the way he's been playing.
"If he can keep doing what he's doing, hopefully he'll get his chance.
"I think Strachan knows what we can do by now, so it's up to him whether he wants to put us in the squad."


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Eg.Heathrow Airport or TW6 1AP
Eg.TW6 1AP or Heathrow Airport
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The National Tourism Administration issued a "national global tourism demonstration zone to create work – Guide" in September 10th, in Ningxia city centre held the second national conference of promoting global tourism, the National Tourism Administration issued a "guideline for the work of creating the national comprehensive tourism demonstration area" (Trial) (hereinafter referred to as the "guide"). The "guide" from "what", "why" and "how to do", "what kind of" four aspects of China's global tourism work requirements. In the "what", "guide", refers to the global tourism sector to grasp tourism to catch party planning tourism, from the tourist attractions to tourist destinations, from the closed type to open type small travel "Travel +" from the "militia" public security management to global governance according to law, from the enterprise's exclusive to the whole society sharing. In the "why", "guide" is put forward, the global tourism is a comprehensive implementation of the five carrier of the development of the concept of global tourism, adapt to the requirements of the era of mass tourism, is an important starting point to promote the reform of the supply side, but also the regional development strategy and positioning. In the "how to do", "guide" is proposed, to improve the comprehensive construction of tourism management system, more comprehensive regulation of tourism public service, establishing tourism industry system, fusion construct open tourism marketing system, promote the tourism leading one, improve the mechanism of interest sharing. In the "what", "guide" is put forward, the global tourism should be a comprehensive management system in place, the toilet revolution effect significantly, public service facilities, urban and rural tourism environment, policies and measures to safeguard and comprehensive, civilized and orderly security outstanding contributions. (Li Zhigang Shen Zhongliang)相关的主题文章:
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