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In 2003, the Hampton Lumber Mills subsidiary of Hampton Affiliates Inc. replaced all of it's mechanical steam traps at six sites in Washington with Enercon's fixed orifice trap systems. It was an aggressive move for one of the Northwest's top lumber manufacturers. Hampton, headquartered in Portland, Ore., produces a billion BF of Douglas fir and hemlock lumber annually at its Oregon and Washington mills. It committed to Enercon's orifice device despite the fact most steam traps in this country are traditional mechanical types.
"When we where approached by Enercon in early 2003," says Angus Low, Kiln/Boiler Lead for Hampton's Cowlitz Div. in Washingtons," we were understandably skeptical. Admittedly, the benefits of a permanent, maintenance-free trap system that produced fuel savings and operational efficiencies was very attractive. Using less fuel reduces pollutants, which is particularly important to an environmentally sensitive company like Hampton."
Low says there was a lot of negative material about orifice installations and technology, particularly on the internet. But Hampton is always looking for ways to improve operations, product quality and profits, and the company has always been open to new ideas and different technologies.
"Instead of a knee-jerk reaction, we decided the Enercon system merited a closer look." Low says. "We'd depend on our own evaluation to accept or reject it. As I reviewed the dynamics of steam, it became obvious that with Enercon custom designing each unit to my application and purpose, this way of trapping steam made sense."
Evaluating the Enercon project was the responsibility of a group that included Low, Operations Manager Harvey Chandler, Steve Evans, Planner/Boiler Supervisor at the Randall, Wash. plant, and Joe Hellem, Quality Control Supervisor of the Randle and Morton, Wash. plants.
"Hampton encourages employees to look for better ways to do things," says Hellem. "We evaluate new ideas carefully and objectively, no matter how off-the-wall they seem. Sure, there's always uncertainty when you're trying something new, but if a company doesn't continually seek better ways to operate, it eventually loses ground to competitors. After doing our homework and assessing the pros and cons, our collective opinion was the system would work for Hampton."
"From a maintanance perspective," adds Evans, "it looked like a good bet because it was permanent. We'd no longer have to inspect traps to see if they were okey, and continually invest labor and money to change out or repair them. That meant our maintenance people could better utilize their time."
Evans notes, or example, whenever temperature or other steam-elated problems occurred, they always looked at the traps first, wondering whether or not they had to repair or change them. As a results, they probably changed traps that were good because they assumed they were the root of the problem. Now they wouldn't have to think of the traps, but the focus on other areas.
Although the technical group had approved the Enercon system, the units still had to prove themselves in actual plant environment. The initial field test was in the 100 psi Hampton drying operations at Morton. This involved replacing 34 mechanical traps in two kilns with Enercon units.
The changeout improved heat transfer while dropping steam pressure from 100 to 90 psi. Diesel fuel needs were cut by nine gallons/hour. "Before installing the orifice traps," says Low, "our combined average kiln time was 67 hours. After installation it was 52 hours."
Based on the results of the six-moth trial starting in March 2003, Hampton proceeded to convert all kilns at five other operations. Over the next five months, Hampton replaced the mechanical traps the kilns at a second Morton site and at its operations in Darrington, Packwood, Randle and Arlington, Wash.. The number of kilns at those locations ranged from two to 20. Each kiln required an average of 20 Enercon units.
"At Packwood," sats Low, "we saw a six-hour (combined average) improvement in the time required to fry a charge of lumber. We where also able to run an additional kiln, which is something we couldn't do before. Overall, our kiln and boiler performance became much more efficient."
Evans points out that at the Randle mill steam goes through the boiler and kilns. "Our target press," he says, "is 110psi. Before the orifice traps were installed, we averaged 98. Since the installation we've averaged 105 psi, a 7 lb. improvement. Average steam flow at the boiler actually increased by 633 lbs. The operators say there's a noticeable difference in the volume of ash we now produce. Makes sense. If the kilns are maintaining a higher pressure, the boiler's not working so hard."
"We challange suppliers to provide meaningful service and backup," says Hellem. "Enercon just didn't sell us parts, they supplied design, technical backup, steam system expertise and trouble shooting. They contacted us continually for updates on how the parts were working."
At Randle, says Hellem, the Enercon technician made suggestions which, when implemented, saved 5,324 gallons of water a day, which at 360 operating days a year is 1.9 million gallons annually. This also cut water treatment costs and has had a significant environmental impact.
"One of the objections we had seen repeatedly on the internet and heard from numerous other sources," says Low, "is that the orifice is only effective in applications that produce steady, fixed condensate loads. This concerned us because our operations are all 'zone control' and we have a constant variable load situation. Based on a year's operations, these (orifice) traps perform with excellence in our varying load environment."
Low notes that a notable improvement had been the heat released in the kiln coils, which has dropped kiln cycle times significantly. There is now uniformity throughout the kilns. This has brought a new level of consistency to the product, which in turn has led to better planer performance and grade recovery. One operation which boiler-wise was very steam challenged has had better, more content steam delivery to the kiln with less flow demand.
'With 22 dry kilns and five boilers to manage, I don't have time for gimmicks," Low says. "We're now able to make better use of our people by focusing on product improvement and process efficiency. Instead of second-guessing traps and having to plant rap maintenance, we can concentrate on proving our process. Proper trapping is really the heart of a good steam process."
Enercon collaborated with Hampton Affiliates in the development of this article. For more information or to arrange a steam system survey, contact your Regional Distributor.
Often overlooked in today's high-tech environment, the steam trip is critical to an officiant steam system. The job of both Enercon's fixed orifice until and traditional trap is to keep the steam in the system and get ride of condensate, which is formed continuously as steam gives up it's latent heat and condenses into water. Purging condensate quickly and officially is critical because water reduces heat transfer and causes erosion, corrosion and water hammer.
A traditional steam trap is designed to open in the presence of condensate and close in the presence of steam. Internal floats, buckets, bimetal, bellows or discs provide the open/shut mechanism. On a 24-hour-a-day application, a mechanical trap will cycle several times a minute and a couple of million times a year.
With Enercon's one-price fixed orifice, steam traveling at the speed of sound continuously forces much slower condensate (about 30MPH) through a precisely sized hole, effectively blocking steam from escaping. The Enercon unit sized to handle 100% of the condensate load produced by a particular application. At 100% capacity, a accurately sized orifice device loses no steam and backs up no condensate. However, there's a common perception that when the load drops below 100% capacity, the orifice device loses inordinate amounts of steam. This is not true.
Every condensate load varies to come event. When the load drops below 100% capacity, the ofifice passes a violently turbulent mixture of equivalent volumes of steam and water, a well-documented phenomenon called two-phase flow. Since condensate is several hundred times denser that steam, steam loss by weight is negligible.
For instance, in a 100 psi system producing 375 lbs./hr. condensate, an accurately sized Enercon unit loses 1.76 lbs./hr. steam if load drops to 25%, which it may never do or even then only sporadically. At 100 psi, according to both Dept. of Energy and trap companies' 'Leaking Steam Trap Discharge Rate' charts, a failed conventional trap (1/8 in.) loses 52.8 lbs/hr.
For a 100 psi mechanical trap system to achieve a less than 5% failure rate, according to the DOE Energy Tips (6-99), monthly to quarterly inspections are recommended. For high-pressure systems (150 psi and above), weekly to monthly inspections are recommended. "Even for companies with annual test-and-replace programs," according to a Textile World report, "studies show the average trap has been leaking for six months before it is replaced."
Enercon recommends it's units for applications where the load drops as low as 25% of capacity, although sources such as the U.S. Naval Facilities Engineering Command Supplements (10-90 and 1-92) say "a fixed orifice sized for a 100% load operated efficiently down to a 10% load." Enercon reports it has replaced an average 99% of the mechanical traps in the 190-plus plants it has converted since 1989.


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A nearly two-year investigation into Russian efforts to influence the 2016 election "does not exonerate" President Trump, Attorney General William Barr said in a summary of the Mueller report on Sunday, as Democrats consider whether there is enough evidence to impeach the president. NewsHour politics correspondent Lisa Desjardins joins Hari Sreenivasan for a look at what Congress may do next.
Aired: 03/24/19
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English homework: write a ghost story, 1996.
This day I needn't to get up too early, because I didn't feel well, so my Mummy said to me, that I could stay at home. When I woke up Mummy and Daddy were already at work. My older sister wasn't at home also. I think she was somewhere with her new boyfriend. I think he is slighty curious. He is dark, nearly black. No, I'm not racist, but I think it is quite unusual -- for example he is invisible in the night... Never mind. My name is Adrian and unlike my sister I must to go to school (except today, of course). Our family lives in a smaller town in a beautiful old house. We live at the top floor, maybe because my father is very interested in heights and flying.
In our house there are several unused flats without tenants, because anybody didn't want them, and I think they are still full of many interesting things. I always wanted to explore secrets of those little bit mysterious flats, but I haven't enough courage. Maybe I can do it with a friend, but I haven't any in the house, because my father forbided it to me. But today I have a great opportunity. Because my "illness" wasn't too serious, I started preparing for my expedition.
Before I come out of the door, I look round very carefully, becouse it could be very embarrassing if somebody caught me. Everything was OK, so I got start on my journey. I ran at terminal velocity through a dark corridor down the stairs. I was directed to a door of one abandoned flat. I went through the door into the flat. I was absolutely fascinated. The flat was full of old furniture and all sorts of things. Everything was covered with webs. I went to the next room and then I saw two boys standing in front of some macabre painting.
"I am very lucky, that our parents went to the theatre. We can make big discoveries here", said the first.
"Of course", said the second, "I hope they will not be back too early. Because..."
"Spectre!!!", screamed first.
I started looking for a spectre, but I couldn't find it.
"Spectre! Help me (anybody)!", shouted both.
I looked at boys and their eyes were fixed on me!
What? What did he say? Spectre?
"Hey, I'm not a spectre!", said I.
"Ghost! Help me (anyway)!", shrieked both again.
"So what? I am. And I am proud of it!"
The ran away. Dolts! It made no sense to continue exploriring. I want back home. I didn't say anybody about my new experience.
Our family lives in a smaller town in a beautiful old house. We live at the top floor, maybe because my father is very interested in heights and flying. This week he won a prize in "Ghost altitude speed races". Mother baked Dead man's little heads. Maybe I'll have an uncle. My sister is in a big love. And my mother is baking Dead man's little heads.
čtvrtek 22. října 2009
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"La Llorona" is also known as the "Weeping Women". La Llorona is a story about a woman who killed her kids by drowning them in the river. Its said that she goes around crying out "Donde estan mis Hijos" which in English means where are my kids. This myth/story has been told for centuries but its told in many ways, but the main use is to scare kids in to always doing the right thing. In today's era La Llorona has been told in many ways so, that people are able to understand the story. The ones that stood out the most was the American version of the story, the movie and a book of other stories that includes of other similar monsters started.
The origins of La Llorona came from Mexico and then it was brought to the United States. The first person to write about La Llorona was Joe Hayes in 1987 (Hayes). Hayes wrote this story very different to what I was told as a little boy. He wrote this story and described the main character as an egotistic person who believes that she Is better than any of the other people in her village (Hayes 1). Hayes described her as the most beautiful woman and increased but, so did her ego. She then falls in love with a handsome Spanish rancher. He works so hard to get her to love him and she plays it off that she doesn't like him but, she really does (Hayes 2). They soon fall in love for each other and have two kids he leaves and only comes to see the kids he ends up cheating on the mother of the kids with a Spanish women with that she gets mad and throws the kids into the river and they are swept away by the current she soon realized what she did and went after them but it was too late (Hayes 3). She then dies the next day and from that point she roams by the river looking for kids and takes the kids that are by themselves. The way that the story is written very different and is making the women look bad.
The second way that the story of La Llorona is being told is by a kid's movie that came out in 2011 and was written by Ricardo Arnaiz. Here the story of La Llorona is being told the same way I was told as a kid. The story goes the same way there is a mother with her two kids that drown by falling off the boat and she couldn't safe them she goes looking for them and ends up dying. Also, instead of her roaming the river she goes into the village looking for her kids and takes all the kids that are left alone and taking them away. This story is very different from the one Joe Hayes wrote. The he wrote is more gruesome and dark while the movie is more kid friendly and is being used as lesson to teach them to love and care for one another and not be selfish. But over all the story still share similar story lines but they share different endings in Joe Hayes she is still roaming looking for her kids while in the movie her spirit sees what horrible things she has done and becomes a happy sole and goes to a better after life. The way that this movie is being written is so that the children can learn for the mistakes of the kids in the movie.
The third way that the story is being told is through an episode of "Grimm" the episode is being told the same way that Joe Hayes wrote the American version. But the episode is being more viewer friendly, but they switch it up just a little instead of La Llorona coming out everyday looking for her kids she only comes out during Halloween and instead of having two kids she has three and takes them by the hand and drowning all together. Here the main character must find a way to defeat the monster and must do it quick or more lives will be lost. This way that the story is being told is different from the other stories the monster must be defeated to bring back the lives that she took with her. This story is dark just like the Written version of Joe Hayes but then again, the episode must viewer friendly.
The these that best fits the monster is "The monster always comes back" the story of La Llorona has been always been brought up and comes back a different way whether it be movie, episode, and a story. Also, the story can always be told in many ways and understood differently as well but over all the story share similar stories lines and characteristics. The story can be used as life lesson to teach the young ones right from wrong. The one thing that the story share is that the monster well always come back to look for her kids and will do what ever it takes to get her kids no matter what she must do.
In closing, the story of La Llorona has been told different throughout the centuries and has been told a different way so that the one telling the story and the one listening is able to understand what the story teller is trying to tell. An that's that a mother will do what ever it takes to have her kids even if it takes her own life. It also tells us that one must not trust one's ego because then it well com back to bite is in the ass and will make everyone that surrounds us say that we are egoistic and only care about ourselves. Because that's what the women who is La Llorona is being showed as in all the stories she only cares for her kids and doesn't care whether she hurts another family by take their kid. But over all La Llorona will always be monster because she is hurting parents by taking their kids and she has no remorse about what she does because she is a monster and monsters don't think when they are doing their monster things.
Works Cited
Hayes Joe "The Weeping Woman" 1987
Is a story that is written in 1987 and is translated from Spanish to English I used it as my main source to compare the movie and the episode and how the stories are different.
La leyenda de la Llorona, Dir. Ricardo Arnaiz, SfGate, 2013
Is kid's movie that I used to show the difference between the story and the episode and saw that the movie was more for kids instead of adults
Grimm La Llorona, twenty-four minutes, 2013
This is an episode of my favorite series in the world in which they grabbed the viewers attention using La llorona to show that all monsters that the main character fights will not be defeated so easily.


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ORBilu Community: Life sciences http://hdl.handle.net/10993/134 The Community's search engine Search this channel search http://orbilu.uni.lu/simple-search Towards the network-based prediction of repurposed drugs using patient-specific metabolic models. http://hdl.handle.net/10993/39354 Title: Towards the network-based prediction of repurposed drugs using patient-specific metabolic models.

Author, co-author: Pacheco, Maria Pires; Bintener, Tamara Jean Rita; Sauter, Thomas Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids http://hdl.handle.net/10993/39342 Title: Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids

Author, co-author: Smith, Lisa M.; Magni, Stefano; Grzyb, Kamil; Antony, Paul; Krüger, Rejko; Skupin, Alexander; Bolognin, Silvia; Schwamborn, Jens Christian

Abstract: Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA-sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, and glutamatergic neurons. Recapitulating these in vivo features makes hMOs an excellent tool for in vitro disease phenotyping and drug discovery. Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality http://hdl.handle.net/10993/39338 Title: Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality

Author, co-author: Walter, Jonas; Bolognin, Silvia; Antony, Paul; Nickels, Sarah; Poovathhingal, Suresh; Salamanca, Luis; Magni, Stefano; Perfeito, Rita; Hoel, Fredrik; Qing, Xiaobing; Jarazo, Javier; Arias-Fuenzalida, Jonathan; Ignac, Tomasz; Monzel, Anna Sophia; Gonzalez-Cano, Laura; Pereira de Almeida, Luis; Skupin, Alexander; Tronstad, Karl; Schwamborn, Jens Christian Novel Insight into the Role of the S100A8/A9 Protein Complex in the Regulation of Neutrophil Functions http://hdl.handle.net/10993/39335 Title: Novel Insight into the Role of the S100A8/A9 Protein Complex in the Regulation of Neutrophil Functions

Author, co-author: Jung, Nicolas

Abstract: S100A8 and S100A9 are members of the S100 family of cytoplasmic EF-hand calcium-binding proteins and are abundantly expressed in the cytosol of neutrophils. Mostly found under heterodimeric form, S100A8/A9 have various intracellular and extracellular functions; they act as alarmins, amplifying the host inflammatory response. Our previous study showed that the intracellular activity of S100A8/A9 is carried by the phosphorylation of S100A9. Based on these results, we further investigated the importance of this post-translational modification on the extracellular activity of the protein complex and its impact on the inflammatory functions of neutrophils. First, we analyzed the phosphorylation state of secreted S100A8/A9 and the mechanism by which the protein complex is released into the extracellular space. Our results show that S100A9 is secreted under a phosphorylated form within the S100A8/A9 protein complex and this release is highly correlated to the process of NETosis. Next, we investigated the inflammatory response of neutrophil-like dHL-60 cells when stimulated with the phosphorylated and non-phosphorylated form of S100A8/A9. Our results indicate that only the phosphorylated form of S100A8/A9 increases the expression and secretion of various cytokines (e.g. TNFa, CCL4, CXCL8). Using receptor-neutralizing antibodies, we then determined the receptor and signaling pathways associated to S100A8/A9-P-induced cytokine secretion. The reduction of expression levels of the previously mentioned cytokines, after TLR4 blocking, point out that S100A8/A9-P-induced signaling is mediated in part by TLR4. Finally, we investigated the post-transcriptional response induced by S100A8/A9-P stimulation. Using miRNA-sequencing of S100A8/A9-P stimulated dHL-60 cells, we identified an upregulation of miR-146a-5p, miR-146b-5p and miR-155-5p expression. Since these three microRNAs have previously been described to regulate TLR4 signaling at various levels, we investigated their influence on the inflammatory response mediated by S100A8/A9-P. Stable overexpression of miR-146a-5p and miR-155-5p in dHL-60 cells resulted in the reduced S100A8/A9-P-mediated secretion of cytokines through the inhibition of key players in the TLR4 signaling pathways. To summarize, our results give new insight into the pro-inflammatory functions induced by S100A8/A9-P in neutrophils and reveal the potential of the phosphorylated protein complex as a major regulator of inflammation in chronic inflammatory diseases. Modeling Parkinson's disease in midbrain-like organoids http://hdl.handle.net/10993/39312 Title: Modeling Parkinson's disease in midbrain-like organoids

Author, co-author: Smits, Lisa; Reinhardt, Lydia; Reinhardt, Peter; Glatza, Michael; Monzel, Anna Sophia; Stanslowsky, Nancy; Rosato-Siri, Marcelo; Zanon, Alessandra; Antony, Paul; Bellmann, Jessica; Nicklas, Sarah; Hemmer, Kathrin; Qing, Xiaobing; Berger, Emanuel; Kalmbach, Norman; Ehrlich, Marc; Bolognin, Silvia; Hicks, Andrew; Wegner, Florian; Sterneckert, Jared; Schwamborn, Jens Christian Statistical Analysis of Piglet Stillbirth http://hdl.handle.net/10993/39295 Title: Statistical Analysis of Piglet Stillbirth

Author, co-author: Canario, L.; Cantoni, E.; Le Bihan, Etienne; Billon, Y.; Bidanel, J.-P.; Foulley, J.-L. Between-breed variability of stillbirth and its relationship with sow and piglet characteristics http://hdl.handle.net/10993/39293 Title: Between-breed variability of stillbirth and its relationship with sow and piglet characteristics

Author, co-author: Canario, Laurianne; Cantoni, E.; Le Bihan, Etienne; Caritez, J.-C.; Billon; Bidanel, J.-P.; Foulley, J.-L.

Abstract: Litter characteristics at birth were recorded in 4 genetic types of sows with differing maternal abilities. Eighty-two litters from F1 Duroc × Large White sows, 651 litters from Large White sows, 63 litters from Meishan sows, and 173 litters from Laconie sows were considered. Statistical models included random effects of sow, litter, or both; fixed effects of sow genetic type, parity, birth assistance, and piglet sex, as well as gestation length, farrowing duration, piglet birth weight, and litter size as linear covariates. The quadratic components of the last 2 factors were also considered. For statistical analyses, GLM were first considered, assuming a binomial distribution of stillbirth. Hierarchical models were also fitted to the data to take into account correlations among piglets from the same litter. Model selection was performed based on deviance and deviance information criterion. Finally, standard and robust generalized estimating equations (GEE) procedures were applied to quantify the importance of each effect on a piglet's probability of stillbirth. The 5 most important factors involved were, in decreasing order (contribution of each effect to variance reduction): difference between piglet birth weight and the litter mean (2.36%), individual birth weight (2.25%), piglet sex (1.01%), farrowing duration (0.99%), and sow genetic type (0.94%). Probability of stillbirth was greater for lighter piglets, for male piglets, and for piglets from small or very large litters. Probability of stillbirth increased with sow parity number and with farrowing duration. Piglets born from Meishan sows had a lower risk of stillbirth (P < 0.0001) and were little affected by the sources of variation mentioned above compared with the 3 other sow genetic types. Standard and robust GEE approaches gave similar results despite some disequilibrium in the data set structure highlighted with the robust GEE approach. An integrative method to predict signallingperturbations for cellular transitions http://hdl.handle.net/10993/39285 Title: An integrative method to predict signallingperturbations for cellular transitions

Author, co-author: Zaffaroni, Gaia; Okawa, Satoshi; Morales-Ruiz, Manuel; del Sol Mesa, Antonio Data and knowledge management in translational research: implementation of the eTRIKS platform for the IMI OncoTrack consortium http://hdl.handle.net/10993/39283 Title: Data and knowledge management in translational research: implementation of the eTRIKS platform for the IMI OncoTrack consortium

Author, co-author: Gu, Wei; Yildirimman, Reha; Van der Stuyft, Emmanuel; Verbeeck, Denny; Herzinger, Sascha; Satagopam, Venkata; Barbosa-Silva, Adriano; Schneider, Reinhard; Lange, Bodo; Lehrach, Hans; Guo, Yike; Henderson, David; Rowe, Anthony; on behalf of the IMI OncoTrack and the IMI eTRIKS consortia

Abstract: For large international research consortia, such as those funded by the European Union's Horizon 2020 programme or the Innovative Medicines Initiative, good data coordination practices and tools are essential for the successful collection, organization and analysis of the resulting data. Research consortia are attempting ever more ambitious science to better understand disease, by leveraging technologies such as whole genome sequencing, proteomics, patient-derived biological models and computer-based systems biology simulations. Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0. http://hdl.handle.net/10993/39264 Title: Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0.

Author, co-author: Heirendt, Laurent; Arreckx, Sylvain; Pfau, Thomas; Mendoza, Sebastian N.; Richelle, Anne; Heinken, Almut Katrin; Haraldsdottir, Hulda S.; Wachowiak, Jacek; Keating, Sarah M.; Vlasov, Vanja; Magnusdottir, Stefania; Ng, Chiam Yu; Preciat, German; Zagare, Alise; Chan, Siu H. J.; Aurich, Maike K.; Clancy, Catherine M.; Modamio, Jennifer; Sauls, John T.; Noronha, Alberto; Bordbar, Aarash; Cousins, Benjamin; El Assal, Diana Charles; Valcarcel, Luis V.; Apaolaza, Inigo; Ghaderi, Susan; Ahookhosh, Masoud; Ben Guebila, Marouen; Kostromins, Andrejs; Sompairac, Nicolas; Le, Hoai M.; Ma, Ding; Sun, Yuekai; Wang, Lin; Yurkovich, James T.; Oliveira, Miguel A. P.; Vuong, Phan T.; El Assal, Lemmer P.; Kuperstein, Inna; Zinovyev, Andrei; Hinton, H. Scott; Bryant, William A.; Aragon Artacho, Francisco J.; Planes, Francisco J.; Stalidzans, Egils; Maass, Alejandro; Vempala, Santosh; Hucka, Michael; Saunders, Michael A.; Maranas, Costas D.; Lewis, Nathan E.; Sauter, Thomas; Palsson, Bernhard O.; Thiele, Ines; Fleming, Ronan M. T.

Abstract: Constraint-based reconstruction and analysis (COBRA) provides a molecular mechanistic framework for integrative analysis of experimental molecular systems biology data and quantitative prediction of physicochemically and biochemically feasible phenotypic states. The COBRA Toolbox is a comprehensive desktop software suite of interoperable COBRA methods. It has found widespread application in biology, biomedicine, and biotechnology because its functions can be flexibly combined to implement tailored COBRA protocols for any biochemical network. This protocol is an update to the COBRA Toolbox v.1.0 and v.2.0. Version 3.0 includes new methods for quality-controlled reconstruction, modeling, topological analysis, strain and experimental design, and network visualization, as well as network integration of chemoinformatic, metabolomic, transcriptomic, proteomic, and thermochemical data. New multi-lingual code integration also enables an expansion in COBRA application scope via high-precision, high-performance, and nonlinear numerical optimization solvers for multi-scale, multi-cellular, and reaction kinetic modeling, respectively. This protocol provides an overview of all these new features and can be adapted to generate and analyze constraint-based models in a wide variety of scenarios. The COBRA Toolbox v.3.0 provides an unparalleled depth of COBRA methods. Hemap: An nteractive online resource for characterizing molecular phenotypes across hematologic malignancies http://hdl.handle.net/10993/39263 Title: Hemap: An nteractive online resource for characterizing molecular phenotypes across hematologic malignancies

Author, co-author: Pölönen, Petri; Mehtonen, Juha; Lin, Jake; Liuksiala, Thomas; Häyrynen, Sergei; Teppo, Susanna; Mäkinen, Artturi; Kumar, Ashwini; Malani, Disha; Pohjolainen, Virva; Porkka, Kimmo; Heckman, Caroline A.; May, Patrick; Hautamäki, Ville; Granberg, Kirsi; Lohi, Olli; Nykter, Matti; Heinäniemi, Merja

Abstract: Large collections of genome-wide data can facilitate the characterization of disease states and subtypes, permitting pan-cancer analysis of molecular phenotypes and evaluation of disease contexts for new therapeutic approaches. We analyzed 9,544 transcriptomes from over 30 hematologic malignancies, normal blood cell types and cell lines, and show that the disease types can be stratified in a data-driven manner. We utilized the obtained molecular clustering for discovery of cluster-specific pathway activity, new biomarkers and in silico drug target prioritization through integration with drug target databases. Using known vulnerabilities and available drug screens in benchmarking, we highlight the importance of integrating the molecular phenotype context and drug target expression for in silico prediction of drug responsiveness. Our analysis implicates BCL2 expression level as important indicator of venetoclax responsiveness and provides a rationale for its targeting in specific leukemia subtypes and multiple myeloma, links several polycomb group proteins that could be targeted by small molecules (SFMBT1, CBX7 and EZH1) with CLL, and supports CDK6 as disease-specific target in AML. Through integration with proteomics data, we characterized target protein expression for pre-B leukemia immunotherapy candidates, including DPEP1. These molecular data can be explored using our freely available interactive resource, Hemap, for expediting therapeutic innovations in hematologic malignancies. Circadian preference as a typology: Latent-class analysis of adolescents' morningness/eveningness, relation with sleep behavior, and with academic outcomes http://hdl.handle.net/10993/39260 Title: Circadian preference as a typology: Latent-class analysis of adolescents' morningness/eveningness, relation with sleep behavior, and with academic outcomes

Author, co-author: Preckel, Franzis; Fischbach, Antoine; Scherrer, Vsevolod; Brunner, Martin; Ugen, Sonja; Lipnevich, Anastasiya A.; Roberts, Richard D. MODELING HUMAN METABOLISM: A DYNAMIC MULTI-TISSUE APPROACH http://hdl.handle.net/10993/39143 Title: MODELING HUMAN METABOLISM: A DYNAMIC MULTI-TISSUE APPROACH

Author, co-author: Martins Conde, Patricia

Abstract: Despite significant advances in constraint-based modelling, a methodology for modelling dynamic multi-tissue models of human metabolism is still missing. Additionally, prior to analysing diseased models, it is important to develop a good methodology, as it would not only enable us to capture the effects of metabolism-associated diseases, but it would also allow us to recapitulate known physiological healthy properties of human metabolism. Therefore, a dynamic multi-tissue model using a new methodology was developed. The objective function comprises a set of complex functions that the multi-tissue model needs to perform. To demonstrate the capabilities of this new approach, different healthy, and unhealthy conditions were simulated. In a first step, the effect of different healthy conditions was analysed (i.e. the fasting, the ingestion of different meals, and exercising at various intensities, and conditions), demonstrating the model's capability to correctly predict metabolic changes occurring on energy-associated pathways. In the second step, biomarkers for a range of inborn errors of metabolism were predicted, and the predictions were shown to be in good agreement with previous data. Finally, after verifying the capability of the dynamic multi-tissue model to review known physiological aspects of human metabolism, this model was further integrated with a physiologically- based pharmacokinetic model of glucose metabolism, previously developed by Schaller et al. (2013). Contrasting conditions, such as healthy and diabetic, were simulated using the multi-scale model during fasting and after an oral glucose tolerance test and candidate drugs to treat type 2 diabetes mellitus were predicted. Five out of the 80 simulated drug targets were predicted as candidate anti-diabetic targets, and the majority of drugs known to inhibit the predicted drug targets, have already been shown to have anti-diabetic effects. The developed approach can be applied to any metabolic disease and to any system where homeostasis plays an important role, or where a simple biomass optimization function is not applicable. Furthermore, the large amount of data collected for the multi-tissue model generation is of significant value for tissue constraint-based metabolic modellers who need data to constrain their models. Targeted DNA repair gene expression study in Glioblastoma patient biopsies: Clinical impact and characterization of the NEIL3 glycosylase, a novel candidate target for therapy http://hdl.handle.net/10993/39115 Title: Targeted DNA repair gene expression study in Glioblastoma patient biopsies: Clinical impact and characterization of the NEIL3 glycosylase, a novel candidate target for therapy

Author, co-author: Gobin, Matthieu Linear system identification from ensemble snapshot observations http://hdl.handle.net/10993/39109 Title: Linear system identification from ensemble snapshot observations

Author, co-author: Aalto, Atte; Goncalves, Jorge

Abstract: Developments in transcriptomics techniques have caused a large demand in tailored computational methods for modelling gene expression dynamics from experimental data. Recently, so-called single-cell experiments have revolutionised genetic studies. These experiments yield gene expression data in single cell resolution for a large number of cells at a time. However, the cells are destroyed in the measurement process, and so the data consist of snapshots of an ensemble evolving over time, instead of time series. The problem studied in this article is how such data can be used in modelling gene regulatory dynamics. Two different paradigms are studied for linear system identification. The first is based on tracking the evolution of the distribution of cells over time. The second is based on the so-called pseudotime concept, identifying a common trajectory through the state space, along which cells propagate with different rates. Therefore, at any given time, the population contains cells in different stages of the trajectory. Resulting methods are compared in numerical experiments. Guidelines for Fluorescent Guided Biallelic HDR Targeting Selection With PiggyBac System Removal for Gene Editing http://hdl.handle.net/10993/39034 Title: Guidelines for Fluorescent Guided Biallelic HDR Targeting Selection With PiggyBac System Removal for Gene Editing

Author, co-author: Jarazo, Javier; Qing, Xiaobing; Schwamborn, Jens Christian High-throughput amenable fluorescence-assays to screen for calmodulin-inhibitors http://hdl.handle.net/10993/38999 Title: High-throughput amenable fluorescence-assays to screen for calmodulin-inhibitors

Author, co-author: Manoharan, Ganesh Babu; Kopra, Kari; Eskonen, Ville; Harma, Harri; Abankwa, Daniel

Abstract: The KRAS gene is highly mutated in human cancers and the focus of current Ras drug development efforts. Recently the interface between the C-terminus of K-Ras and calmodulin (CaM) was proposed as a target site to block K-Ras driven cancer cell stemness. We therefore aimed at developing a high-throughput amenable screening assay to identify novel CaM-inhibitors as potential K-Ras stemness-signaling disruptors. A modulated time-resolved Förster resonance energy transfer (mTR-FRET)-assay was developed and benchmarked against an identically designed fluorescence anisotropy (FA)-assay. In both assays, two CaM-binding peptides were labeled with Eu(III)-chelate or fluorescein and used as single-label reporter probes that were displaced from CaM upon competitor binding. Thus, peptidic and small molecule competitors with nanomolar to micromolar affinities to CaM could be detected, including a peptide that was derived from the C-terminus of K-Ras. In order to detect CaM-residue specific covalent inhibitors, a cell lysate-based Förster resonance energy transfer (FRET)-assay was furthermore established. This assay enabled us to measure the slow, residue-specific, covalent inhibition by ophiobolin A in the presence of other endogenous proteins. In conclusion, we have developed a panel of fluorescence-assays that allows identification of conventional and covalent CaM-inhibitors as potential disruptors of K-Ras driven cancer cell stemness. Genetic meta-analysis of diagnosed Alzheimer's disease identifies new risk loci and implicates Aβ, tau, immunity and lipid processing http://hdl.handle.net/10993/38969 Title: Genetic meta-analysis of diagnosed Alzheimer's disease identifies new risk loci and implicates Aβ, tau, immunity and lipid processing

Author, co-author: Kunkle, Brian W.; Grenier-Boley, Benjamin; Sims, Rebecca; Bis, Joshua C.; Damotte, Vincent; Naj, Adam C.; Boland, Anne; Vronskaya, Maria; van der Lee, Sven J.; Amlie-Wolf, Alexandre; Bellenguez, Céline; Frizatti, Aura; Chouraki, Vincent; Martin, Eden R.; Sleegers, Kristel; Badarinarayan, Nandini; Jakobsdottir, Johanna; Hamilton-Nelson, Kara L.; Moreno-Grau, Sonia; Olaso, Robert; Raybould, Rachel; Chen, Yuning; Kuzma, Amanda B.; Hiltunen, Mikko; Morgan, Taniesha; Ahmad, Shahzad; Vardarajan, Badri N.; Epelbaum, Jacques; Hoffmann, Per; Boada, Merce; Beecham, Gary W.; Garnier, Jean-Guillaume; Harold, Denise; Fitzpatrick, Annette L.; Valladares, Otto; Moutet, Marie-Laure; Gerrish, Amy; Smith, Albert V.; Qu, Liming; Bacq, Delphine; Denning, Nicola; Jian, Xueqiu; Zhao, Yi; Zompo, Maria Del; Fox, Nick C.; Choi, Seung-Hoan; Mateo, Ignacio; Hughes, Joseph T.; Adams, Hieab H.; Malamon, John; Sanchez-Garcia, Florentino; Patel, Yogen; Brody, Jennifer A.; Dombroski, Beth A.; Naranjo, Maria Candida Deniz; Daniilidou, Makrina; Eiriksdottir, Gudny; Mukherjee, Shubhabrata; Wallon, David; Uphill, James; Aspelund, Thor; Cantwell, Laura B.; Garzia, Fabienne; Galimberti, Daniela; Hofer, Edith; Butkiewicz, Mariusz; Fin, Bertrand; Scarpini, Elio; Sarnowski, Chloe; Bush, Will S.; Meslage, Stéphane; Kornhuber, Johannes; White, Charles C.; Song, Yuenjoo; Barber, Robert C.; Engelborghs, Sebastiaan; Sordon, Sabrina; Voijnovic, Dina; Adams, Perrie M.; Vandenberghe, Rik; Mayhaus, Manuel; Cupples, L. Adrienne; Albert, Marilyn S.; Deyn, Peter P. De; Gu, Wei; Himali, Jayanadra J.; Beekly, Duane; Squassina, Alessio; Hartmann, Annette M.; Orellana, Adelina; Blacker, Deborah; Rodriguez-Rodriguez, Eloy; Lovestone, Simon; Garcia, Melissa E.; Doody, Rachelle S.; Munoz-Fernadez, Carmen; Sussams, Rebecca; Lin, Honghuang; Fairchild, Thomas J.; Benito, Yolanda A.; Holmes, Clive; Karamuji -\vComi, Hata; Frosch, Matthew P.; Thonberg, Hakan; Maier, Wolfgang; Roschupkin, Gena; Ghetti, Bernardino; Giedraitis, Vilmantas; Kawalia, Amit; Li, Shuo; Huebinger, Ryan M.; Kilander, Lena; Moebus, Susanne; Hernández, Isabel; Kamboh, M. Ilyas; Brundin, Rosemarie; Turton, James; Yang, Qiong; Katz, Mindy J.; Concari, Letizia; Lord, Jenny; Beiser, Alexa S.; Keene, C. Dirk; Helisalmi, Seppo; Kloszewska, Iwona; Kukull, Walter A.; Koivisto, Anne Maria; Lynch, Aoibhinn; Tarraga, Lluís; Larson, Eric B.; Haapasalo, Annakaisa; Lawlor, Brian; Mosley, Thomas H.; Lipton, Richard B.; Solfrizzi, Vincenzo; Gill, Michael; Longstreth, W. T.; Montine, Thomas J.; Frisardi, Vincenza; Diez-Fairen, Monica; Rivadeneira, Fernando; Petersen, Ronald C.; Deramecourt, Vincent; Alvarez, Ignacio; Salani, Francesca; Ciaramella, Antonio; Boerwinkle, Eric; Reiman, Eric M.; Fievet, Nathalie; Rotter, Jerome I.; Reisch, Joan S.; Hanon, Olivier; Cupidi, Chiara; Uitterlinden, A. G. 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Abstract: Risk for late-onset Alzheimer's disease (LOAD), the most prevalent dementia, is partially driven by genetics. To identify LOAD risk loci, we performed a large genome-wide association meta-analysis of clinically diagnosed LOAD (94,437 individuals). We confirm 20 previous LOAD risk loci and identify five new genome-wide loci (IQCK, ACE, ADAM10, ADAMTS1, and WWOX), two of which (ADAM10, ACE) were identified in a recent genome-wide association (GWAS)-by-familial-proxy of Alzheimer's or dementia. Fine-mapping of the human leukocyte antigen (HLA) region confirms the neurological and immune-mediated disease haplotype HLA-DR15 as a risk factor for LOAD. Pathway analysis implicates immunity, lipid metabolism, tau binding proteins, and amyloid precursor protein (APP) metabolism, showing that genetic variants affecting APP and A$\beta$ processing are associated not only with early-onset autosomal dominant Alzheimer's disease but also with LOAD. Analyses of risk genes and pathways show enrichment for rare variants (P = 1.32 × 10−7), indicating that additional rare variants remain to be identified. We also identify important genetic correlations between LOAD and traits such as family history of dementia and education. A general computational approach to predicting synergistic transcriptional cores that determine cell subpopulation identities http://hdl.handle.net/10993/38951 Title: A general computational approach to predicting synergistic transcriptional cores that determine cell subpopulation identities

Author, co-author: Okawa, Satoshi; del Sol Mesa, Antonio A general computational approach to predicting synergistic transcriptional cores that determine cellsubpopulation identities http://hdl.handle.net/10993/38950 Title: A general computational approach to predicting synergistic transcriptional cores that determine cellsubpopulation identities

Author, co-author: del Sol Mesa, Antonio


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Contemporary digital media requires a new visual language that would expand the appeal of cultural production beyond the device and into the broader realm of media ecology. Having done a variety of work for a number of international clients and with a career path that led him though world's cultural capitals like Moscow, Dubai, Istanbul and London, Zhenia is now looking for new opportunities that will allow to develop a unique approach to address the global audience. While recognizing the present-day requirement for mobile, tablet and desktop-ready designs, he sees the current state of media as a fluid state of becoming. Having gained a unique skillset throughout his experience with book and magazine publishing, Zhenia has turned his attention to digital media in 2009 and has been working as a data visualisation designer ever since. He approaches critically both citizen journalism, - the potentiality of user-generated content for breaking the mass media's long-established monopoly on representation of reality, - as well as capital-driven corporate statistics. Instead, he proposes to employ data to create narratives that are comprehensible, compelling and relevant within today's porous and multi-faceted medial discourse. Zhenia's sees his potential employment as an important point of connection between his multiple skills as a journalist, designer and researcher. His aims are therefore to learn, explore and champion an emotional, deeply personal, while at the same time tacitly political way of thinking and telling stories through and with cutting edge technology.Locked Pro Plan feature
While employed at Graphic, a London-based specialist data visualisation agency, Zhenia continued pursuing his data visualisation ambition sparked at The Guardian. He proceeded with developing his bespoke style of employing data for storytelling and produced a variety of infographic work, including mobile first and desktop interactive applications, informational social media content, illustration and iconography for clients like TNS, Adobe, London First, Colgate, Unilever, gov.uk and Royal Society of Chemists among others.
While living and working in London Zhenia did freelance data visualisation work for Contently, a New York-based agency specialized in contemporary storytelling for international brands. While working with them, he has delivered mobile, desktop and print static infographics for clients like IBM Watson, NBC Universal, BBH and BBVA.
Zhenia's time at The Guardian instructed him a lot on his approach to visual storytelling. While working there, he was engaged with conceptualisation and delivery of various data visualisation work, including but not limited to: static infographics, mobile-first interfaces, interactive dashboards, illustrations and iconography. The department Zhenia was working in was involved with The Guardian Website projects as well as third party clients, such as Lloyds of London, Ofsted, Accenture, Google and others. One of the self-initiated infographic projects, 39 Stats: Charting Hitchcock Obsessions (http://www.evasilev.com/index/#/hitchcock/) produced by Zhenia in collaboration with the author Adam Frost was awarded Silver at London's Information is Beautiful award in 2013. Besides The Guardian, the Hitchcock project was also later featured on Slate, FastCo:Design and Understanding the World: The Atlas of Infographics by Taschen.
Having moved to London to do his MA in Communication Design, Zhenia kept on working as a freelance designer. During this period, he had delivered a variety of digital data visualisations, web design and illustration projects to clients like Oxford University Press, Sony, Computer Arts and Fox. Throughout this years, Zhenia also did remote freelance illustration for Russia's major information agency, RIA Novosti.
Zhenia gained his initial experience as print designer in Moscow, Russia where he worked and studied after finishing his BA Journalism degree in St.Petersburg State University. In Moscow, Zhenia was lucky enough to work with some of Russia's major book publishing houses, as well as for the Russian franchises of magazines like Time Out, Grazia and Newsweek. At this stage of his career he also undertook work trips to Dubai, AE and Istanbul, TR, where he was also busy with magazine and other design work for print.
This two-year low-residency and research-based MA program has provided the space for reflection and production that has taken Zhenia's visual storytelling work into a new and yet to be explored directions, such as theoretical writing, video work and performativity. Time spent in the Netherlands allowed him to focus on a more instructed approach to projects and was indispensable for initiating his personal research around the involvement of the individual within the context of digital networked media that Zhenia now carries across into his professional design practice.
During his time at Kingston, Zhenia has acquired hands-on skills on illustrative and creative writing techniques. While the course itself was more focused around some more traditional trajectories like illustrations for children's books, graphic novels and editorial content, the program has helped him shape up the approach to storytelling for different audiences and develop methodology for successfully carrying the messages across the multitude of contemporary media.
This year-long evening course in magazine design that Zhenia took after five years of working in publishing had helped him to switch to employment in mass media. With background in journalism, Zhenia has always felt more comfortable working around journalists and within news media organisations.
A Russian five-year specialist study in Journalism (translated differently as BA or MA in Bologna educational model) was formative for Zhenia's writing and communication skills. While he successfully graduated with a thesis on Internet semiotics, his study never resulted in a journalism career. Instead, Zhenia has followed his passion for visual storytelling and started working as a graphic designer immediately after finishing the course.


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Karthik Srinivasan, MathWorks
Program embedded processors to estimate parameters and detect changes in motor dynamics in real time using System Identification Toolbox™.
Online Parameter Estimation Commands
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Motor Control Architecture for Multiple Production Programs
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Embedded Code Generation for AC Motor Controllers
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Feature Detection, Extraction, and Matching with RANSAC
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Crosspost from Opensource.com: How Do We Fix the State of Technical Documentation?
July 19, 2017|Best practices, Crosspost
If we all agree that open source as a whole has a documentation problem, what can we do to work on addressing and fixing it?
How Do We Fix the State of Technical Documentation?
We have to talk about why people would be motivated to fix the problem before we can get them to do so.
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Chapter 5; PART-I
October 27, 1882
Master's boat trip with Keshab:
|Keshab Chandra Sen
IT WAS FRIDAY, the day of the Lakshmi Puja. Keshab Chandra Sen had arranged a boat trip on the Ganges for Sri Ramakrishna.
About four o'clock in the afternoon the steamboat with Keshab and his Brahmo followers cast anchor in the Ganges alongside the Kāli temple at Dakshineswar. The passengers saw in front of them the bathing-ghat and the chandni. To their left, in the temple compound, stood six temples of Śiva, and to their right another group of six Śiva temples. The white steeple of the Kāli temple, the tree-tops of the Panchavati, and the silhouette of pine-trees stood high against the blue autumn sky. The gardens between the two nahabats were filled with fragrant flowers, and along the bank of the Ganges were rows of flowering plants. The blue sky was reflected in the brown water of the river, the sacred Ganges, associated with the most ancient traditions of Aryan civilization. The outer world appeared soft and serene, and the hearts of the Brahmo devotees were filled with peace.
Master in samādhi
Sri Ramakrishna was in his room talking with Vijay and Haralal. Some disciples of Keshab entered. Bowing before the Master, they said to him: "Sir, the steamer has arrived. Keshab Babu has asked us to take you there." A small boat was to carry the Master to the steamer. No sooner did he get into the boat than he lost outer consciousness in samādhi. Vijay was with him.
M. was among the passengers. As the boat came alongside the steamer, all rushed to the railing to have a view of Sri Ramakrishna. Keshab became anxious to get him safely on board. With great difficulty the Master was brought back to consciousness of the world and taken to a cabin in the steamer. Still in an abstracted mood, he walked mechanically, leaning on a devotee for support. Keshab and the others bowed before him, but he was not aware of them. Inside the cabin there were a few chairs and a table. He was made to sit on one of the chairs, Keshab and Vijay occupying two others. Some devotees were also seated, most of them on the floor, while many others had to stand outside. They peered eagerly through the door and windows. Sri Ramakrishna again went into deep samādhi and became totally unconscious of the outer world.
As the air in the room was stuffy because of the crowd of people, Keshab opened the windows. He was embarrassed to meet Vijay (see picture), since they had differed in certain principles of the Brāhrno Samaj and Vijay had separated himself from Keshab's organization, joining another society.
The Brahmo devotees looked wistfully at the Master. Gradually he came back to sense consciousness; but the divine intoxication still lingered. He said to himself in a whisper: "Mother, why have You brought me here? They are hedged around and not free. Can I free them?" Did the Master find that the people assembled there were locked within the prison walls of the world? Did their helplessness make the Master address these words to the Divine Mother?
God dwells in devotee's heart:
Sri Ramakrishna was gradually becoming conscious of the outside world. Nilmadhav of Ghazipur and a Brahmo devotee were talking about Pavhari Baba. Another Brahmo devotee said to the Master: "Sir, these gentlemen visited Pavhari Baba. He lives in Ghazipur. He is a holy man like yourself." The Master could hardly talk; he only smiled. The devotee continued, "Sir, Pavhari Baba keeps your photograph in his room." Pointing to his body the Master said with a smile, "Just a pillow-case."
The Master continued: "But you should remember that the heart of the devotee is the abode of God. He dwells, no doubt, in all beings, but He especially manifests Himself in the heart of the devotee. A landlord may at one time or another visit all parts of his estate, but people say he is generally to be found in a particular drawing-room. The heart of the devotee is the drawing-room of God.
Attitude of jnānis and bhaktās:
"He who is called Brahman by the jnanis is known as Ātman by the yogis and as Bhagavan by the bhaktas. The same brahmin is called priest, when worshipping in the temple, and cook, when preparing a meal in the kitchen. The jnani sticking to the path of knowledge, always reasons about the Reality, saying, 'Not this, not this'. Brahman is neither 'this' nor 'that'; It is neither the universe nor its living beings. Reasoning in this way, the mind becomes steady. Then it disappears and the aspirant goes into samādhi. This is the knowledge of Brahman. It is the unwavering conviction of the jnani that Brahman alone is real and the world illusory. All these names and forms are illusory, like a dream. What Brahman is cannot be described. One cannot even say that Brahman is a Person. This is the opinion of the jnanis, the followers of Vedanta philosophy.
"But the bhaktas accept all the states of consciousness. They take the waking state to be real also. They don't think the world to be illusory, like a dream. They say that the universe is a manifestation of God's power and glory. God has created all these - sky, stars, moon, sun, mountains, ocean, men, animals. They constitute His glory. He is within us, in our hearts. Again, He is outside. The most advanced devotees say that He Himself has become all this - the twenty-four cosmic principles, the universe, and all living beings. The devotee of God wants to eat sugar, not to become sugar. (All laugh.)
"Do you know how a lover of God feels? His attitude is: 'O God, Thou are the Master, and I am Thy servant. Thou art the Mother, and I am Thy child.' Or again: 'Thou art my Father and Mother. Thou art the Whole, and I am a part.' He doesn't like to say, 'I am Brahman.'
Attitude of yogis:
"The yogi seeks to realize the Paramatman, the Supreme Soul. His ideal is the union of the embodied soul and the Supreme Soul. He withdraws his mind from sense-objects and tries to concentrate it on the Paramatman. Therefore, during the first stage of his spiritual discipline, he retires into solitude and with undivided attention practises meditation in a fixed posture.
"But the Reality is one and the same. The difference is only in name. He who is Brahman is verily Ātman, and again, He is the Bhagavan. He is Brahman to the followers of the path of knowledge, Paramatman to the yogis, and Bhagavan to the lovers of God."
|Distant view of Dakshineswar seen from Belur Math Ganga bank
Reasoning of jnanis:
MASTER: "The jnanis, who adhere to the non-dualistic philosophy of Vedanta, say that the acts of creation, preservation, and destruction, the universe itself and all its living beings, are the manifestations of Śakti, the Divine Power. If you reason it out, you will realize that all these are as illusory as a dream. Brahman alone is the Reality, and all else is unreal. Even this very Śakti is unsubstantial, like a dream.
"But though you reason all your life, unless you are established in samādhi, you cannot go beyond the jurisdiction of Śakti. Even when you say, 'I am meditating', or 'I am contemplating', still you are moving in the realm of Śakti, within Its power.
Identity of Brahman and Śakti
"Thus Brahman and Śakti are identical. If you accept the one, you must accept the other. It is like fire and its power to burn. If you see the fire, you must recognize its power to burn also. You cannot think of fire without its power to burn, nor can you think of the power to burn without fire. You cannot conceive of the sun's rays without the sun, nor can you conceive of the sun without its rays.
"What is milk like? Oh, you say, it is something white. You cannot think of the milk without the whiteness, and again, you cannot think of the whiteness without the milk.
"Thus one cannot think of Brahman without Śakti, or of Śakti without Brahman. One cannot think of the Absolute without the Relative, or of the Relative without the Absolute.
"The Primordial Power is ever at play. She is creating, preserving, and destroying in play, as it were. This Power is called Kāli. Kāli is verily Brahman, and Brahman is verily Kāli. It is one and the same Reality. When we think of It as inactive, that is to say, not engaged in the acts of creation, preservation, and destruction, then we call It Brahman. But when It engages in these activities, then we call It Kāli or Śakti. The Reality is one and the same; the difference is in name and form.
"It is like water, called in different languages by different names, such as 'jal', 'pani', and so forth. There are three or four ghats on a lake. The Hindus, who drink water at one place, call it 'jal'. The Mussalmans at another place call it 'pani'. And the English at a third place call it 'water'. All three denote one and the same thing, the difference being in the name only. In the same way, some address the Reality as 'Allah', some as 'God', some as 'Brahman', some as 'Kāli', and others by such names as 'Rama', 'Jesus', 'Durga', 'Hari.' "
SOURCE: The Gospel of Sri Ramakrishna;