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Concept: Adult stem cell
Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing
- Proceedings of the National Academy of Sciences of the United States of America
- Published almost 3 years ago
- Discuss
Biological resurfacing of entire articular surfaces represents an important but challenging strategy for treatment of cartilage degeneration that occurs in osteoarthritis. Not only does this approach require anatomically sized and functional engineered cartilage, but the inflammatory environment within an arthritic joint may also inhibit chondrogenesis and induce degradation of native and engineered cartilage. The goal of this study was to use adult stem cells to engineer anatomically shaped, functional cartilage constructs capable of tunable and inducible expression of antiinflammatory molecules, specifically IL-1 receptor antagonist (IL-1Ra). Large (22-mm-diameter) hemispherical scaffolds were fabricated from 3D woven poly(ε-caprolactone) (PCL) fibers into two different configurations and seeded with human adipose-derived stem cells (ASCs). Doxycycline (dox)-inducible lentiviral vectors containing eGFP or IL-1Ra transgenes were immobilized to the PCL to transduce ASCs upon seeding, and constructs were cultured in chondrogenic conditions for 28 d. Constructs showed biomimetic cartilage properties and uniform tissue growth while maintaining their anatomic shape throughout culture. IL-1Ra-expressing constructs produced nearly 1 µg/mL of IL-1Ra upon controlled induction with dox. Treatment with IL-1 significantly increased matrix metalloprotease activity in the conditioned media of eGFP-expressing constructs but not in IL-1Ra-expressing constructs. Our findings show that advanced textile manufacturing combined with scaffold-mediated gene delivery can be used to tissue engineer large anatomically shaped cartilage constructs that possess controlled delivery of anticytokine therapy. Importantly, these cartilage constructs have the potential to provide mechanical functionality immediately upon implantation, as they will need to replace a majority, if not the entire joint surface to restore function.
EdnrB Governs Regenerative Response of Melanocyte Stem Cells by Crosstalk with Wnt Signaling
- Cell reports
- Published almost 3 years ago
- Discuss
Delineating the crosstalk between distinct signaling pathways is key to understanding the diverse and dynamic responses of adult stem cells during tissue regeneration. Here, we demonstrate that the Edn/EdnrB signaling pathway can interact with other signaling pathways to elicit distinct stem cell functions during tissue regeneration. EdnrB signaling promotes proliferation and differentiation of melanocyte stem cells (McSCs), dramatically enhancing the regeneration of hair and epidermal melanocytes. This effect is dependent upon active Wnt signaling that is initiated by Wnt ligand secretion from the hair follicle epithelial niche. Further, this Wnt-dependent EdnrB signaling can rescue the defects in melanocyte regeneration caused by Mc1R loss. This suggests that targeting Edn/EdnrB signaling in McSCs can be a therapeutic approach to promote photoprotective-melanocyte regeneration, which may be useful for those with increased risk of skin cancers due to Mc1R variants.
Gap Junctional Blockade Stochastically Induces Different Species-Specific Head Anatomies in Genetically Wild-Type Girardia dorotocephala Flatworms
- International journal of molecular sciences
- Published over 3 years ago
- Discuss
The shape of an animal body plan is constructed from protein components encoded by the genome. However, bioelectric networks composed of many cell types have their own intrinsic dynamics, and can drive distinct morphological outcomes during embryogenesis and regeneration. Planarian flatworms are a popular system for exploring body plan patterning due to their regenerative capacity, but despite considerable molecular information regarding stem cell differentiation and basic axial patterning, very little is known about how distinct head shapes are produced. Here, we show that after decapitation in G. dorotocephala, a transient perturbation of physiological connectivity among cells (using the gap junction blocker octanol) can result in regenerated heads with quite different shapes, stochastically matching other known species of planaria (S. mediterranea, D. japonica, and P. felina). We use morphometric analysis to quantify the ability of physiological network perturbations to induce different species-specific head shapes from the same genome. Moreover, we present a computational agent-based model of cell and physical dynamics during regeneration that quantitatively reproduces the observed shape changes. Morphological alterations induced in a genomically wild-type G. dorotocephala during regeneration include not only the shape of the head but also the morphology of the brain, the characteristic distribution of adult stem cells (neoblasts), and the bioelectric gradients of resting potential within the anterior tissues. Interestingly, the shape change is not permanent; after regeneration is complete, intact animals remodel back to G. dorotocephala-appropriate head shape within several weeks in a secondary phase of remodeling following initial complete regeneration. We present a conceptual model to guide future work to delineate the molecular mechanisms by which bioelectric networks stochastically select among a small set of discrete head morphologies. Taken together, these data and analyses shed light on important physiological modifiers of morphological information in dictating species-specific shape, and reveal them to be a novel instructive input into head patterning in regenerating planaria.
Embryonic origin of adult stem cells required for tissue homeostasis and regeneration
Planarian neoblasts are pluripotent, adult somatic stem cells and lineage-primed progenitors required for production and maintenance of all differentiated cell types, including the germline. Neoblasts, originally defined as undifferentiated cells residing in the adult parenchyma, are frequently compared to embryonic stem cells yet their developmental origin remains obscure. We investigated the provenance of neoblasts during S. mediterranea embryogenesis, and report that neoblasts arise from an anarchic, cycling piwi-1+ population wholly responsible for production of all temporary and definitive organs during embryogenesis. Early embryonic piwi-1+ cells are molecularly and functionally distinct from neoblasts: they express unique cohorts of early embryo enriched transcripts and behave differently than neoblasts in cell transplantation assays. Neoblast lineages arise as organogenesis begins and are required for construction of all major organ systems during embryogenesis. These subpopulations are continuously generated during adulthood, where they act as agents of tissue homeostasis and regeneration.
Targeted mRNA Decay by RNA Binding Protein AUF1 Regulates Adult Muscle Stem Cell Fate, Promoting Skeletal Muscle Integrity
- Cell reports
- Published over 2 years ago
- Discuss
Following skeletal muscle injury, muscle stem cells (satellite cells) are activated, proliferate, and differentiate to form myofibers. We show that mRNA-decay protein AUF1 regulates satellite cell function through targeted degradation of specific mRNAs containing 3' AU-rich elements (AREs). auf1(-/-) mice undergo accelerated skeletal muscle wasting with age and impaired skeletal muscle repair following injury. Satellite cell mRNA analysis and regeneration studies demonstrate that auf1(-/-) satellite cell self-renewal is impaired due to increased stability and overexpression of ARE-mRNAs, including cell-autonomous overexpression of matrix metalloprotease MMP9. Secreted MMP9 degrades the skeletal muscle matrix, preventing satellite-cell-mediated regeneration and return to quiescence. Blocking MMP9 activity in auf1(-/-) mice restores skeletal muscle repair and maintenance of the satellite cell population. Control of ARE-mRNA decay by AUF1 represents a mechanism for adult stem cell regulation and is implicated in human skeletal muscle wasting diseases.
Development of the Biopen: a handheld device for surgical printing of adipose stem cells at a chondral wound site
- Biofabrication
- Published about 3 years ago
- Discuss
We present a new approach which aims to translate freeform biofabrication into the surgical field, while staying true to the practical constraints of the operating theatre. Herein we describe the development of a handheld biofabrication tool, dubbed the 'biopen', which enables the deposition of living cells and biomaterials in a manual, direct-write fashion. A gelatin-methacrylamide/hyaluronic acid-methacrylate (GelMa/HAMa) hydrogel was printed and UV crosslinked during the deposition process to generate surgically sculpted 3D structures. Custom titanium nozzles were fabricated to allow printing of multiple ink formulations in a collinear (side-by-side) geometry. Independently applied extrusion pressure for both chambers allows for geometric control of the printed structure and for the creation of compositional gradients. In vitro experiments demonstrated that human adipose stem cells maintain high viability (>97%) one week after biopen printing in GelMa/HAMa hydrogels. The biopen described in this study paves the way for the use of 3D bioprinting during the surgical process. The ability to directly control the deposition of regenerative scaffolds with or without the presence of live cells during the surgical process presents an exciting advance not only in the fields of cartilage and bone regeneration but also in other fields where tissue regeneration and replacement are critical.
Adipose-Derived Stem Cells Retain their Regenerative Potential after Methotrexate Treatment
- Experimental cell research
- Published almost 5 years ago
- Discuss
In musculoskeletal tissues like bone, chemotherapy can impair progenitor cell differentiation and proliferation, resulting in decreased bone growth and mineralization throughout a patient's lifetime. In the current study, we investigated the effects of chemotherapeutics on adipose-derived stem cell (ASC) function to determine whether this cell source could be a candidate for repairing, or even preventing, chemotherapy-induced tissue damage. Dose-dependent proliferation rates of ASCs and normal human fibroblasts (NHFs) were quantified after treatment with cytarabine (CY), etoposide (ETO), methotrexate (MTX), and vincristine (VIN) using a fluorescence-based assay. The influence of MTX on the multipotency of ASCs and freshly isolated stromal vascular fraction (SVF) cells was also evaluated using lineage-specific stains and spectrophotometry. ASC and NHF proliferation were equally inhibited by exposure to CY and ETO; however, when treated with MTX and VIN, ASCs exhibited greater resistance. This was especially apparent for MTX-treated samples, with ASC proliferation showing no inhibition for clinically relevant MTX doses ranging from 0.1 to 50μM. Additional experiments revealed that the differentiation potential of ASCs was not affected by MTX treatment and that upregulation of dihydrofolate reductase possibly contributed to this response. Moreover, SVF cells, which include ASCs, exhibited similar resistance to MTX impairment, with respect to cellular proliferation, clonogenicity, and differentiation capability. Therefore, we have shown that the regenerative properties of ASCs resist the cytotoxicity of MTX, identifying these cells as a potential key for repairing musculoskeletal damage in patients undergoing chemotherapy.
Lgr5-EGFP Marks Taste Bud Stem/Progenitor Cells in Posterior Tongue
- Stem cells (Dayton, Ohio)
- Published about 6 years ago
- Discuss
Until recently, reliable markers for adult stem cells have been lacking for many regenerative mammalian tissues. Lgr5 (leucine-rich repeat-containing G-protein coupled receptor 5) has been identified as a marker for adult stem cells in intestine, stomach, and hair follicle; Lgr5-expressing cells give rise to all types of cells in these tissues. Taste epithelium also regenerates constantly, yet the identity of adult taste stem cells remains elusive. In this study, we found that Lgr5 is strongly expressed in cells at the bottom of trench areas at the base of circumvallate and foliate taste papillae and weakly expressed in the basal area of taste buds and that Lgr5-expressing cells in posterior tongue are a subset of K14-positive epithelial cells. Lineage-tracing experiments using an inducible Cre knock-in allele in combination with Rosa26-LacZ and Rosa26-tdTomato reporter strains showed that Lgr5-expressing cells gave rise to taste cells, perigemmal cells, along with self-renewing cells at the bottom of trench areas at the base of circumvallate and foliate papillae. Moreover, using subtype-specific taste markers, we found that Lgr5-expressing cell progeny include all three major types of adult taste cells. Our results indicate that Lgr5 may mark adult taste stem or progenitor cells in the posterior portion of the tongue.
Oestrogen action on thyroid progenitor cells: relevant for the pathogenesis of thyroid nodules?
- The Journal of endocrinology
- Published almost 6 years ago
- Discuss
Benign and malignant thyroid nodules are more prevalent in females than in men. Experimental data suggest that the proliferative effect of oestrogen rather than polymorphisms is responsible for this gender difference. This study analysed whether both differentiated thyroid cells and thyroid stem and progenitor cells are a target of oestrogen action. In thyroid stem/progenitor cells derived from nodular goitres the ability of 17beta-oestradiol to induce thyrosphere formation, the expression of oestrogen receptors and the effect of 17beta-oestradiol on growth, expression of marker of stem cells and thyroid differentiation (TSH receptor, thyroperoxidase, thyroglobulin, sodium iodide symporter expression) were analysed. 17beta-oestradiol induced thyrosphere formation, albeit to lower extent than other growth factors. Thyroid stem and progenitor cells expressed oestrogen receptor alpha and beta with an 8 time higher expression level of oestrogen receptor alpha mRNA compared to differentiated thyrocytes. 17beta-oestradiol was a potent stimulator of thyroid stem/ progenitor cell growth. In contrast, TSH-induced differentiation of progenitor cells, in particular the expression of the sodium iodide symporter, was significantly inhibited by 17beta-oestradiol.In conclusion, oestrogen stimulated growth and simultaneously inhibited differentiation of thyroid nodules derived stem/progenitor cells. From these data and based on the concept of cellular heterogeneity, we hypothesize a supportive role of oestrogen in the propagation of thyroid stem/progenitor cells leading to a selection of a progeny of growth-prone cells with a decreased differentiation. These cells may be the origin of hypo- or non-functioning thyroid nodules in females.
Analysis for apoptosis and necrosis on adipocytes, stromal vascular fraction, and adipose-derived stem cells in human lipoaspirates after liposuction
- Plastic and reconstructive surgery
- Published over 6 years ago
- Discuss
: Adipose-derived stem cells have become the most studied adult stem cells. The authors examined the apoptosis and necrosis rates for adipocyte, stromal vascular fraction, and adipose-derived stem cells in fresh human lipoaspirates.


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My Content
Membership has its privileges! Sign up or sign in to gain full access to both All About Jazz and Jazz Near You. Learn more.
I love jazz because it is music for the mind, body and soul. I was first exposed to jazz when I started visiting Mike Chadwick's Decoy Records in Manchester (UK) in the late 1980s and then started listening to his Saturday night show 'The Cutting Edge' on Jazz FM. The best concert I ever attended was the Esbjorn Svensson Trio at London's Pizza Express Jazz Club in October 1999... amazing and sublime. The first jazz record I bought was a Best of Blue Note sampler. My advice to new listeners: start with some Blue Note albums--Lou Donaldson, Hank Mobley, Stanley Turrentine--and then come right up to date by listening to Mike Chadwick's European Jazz Union show on soulandjazz.com. Without him knowing it, Mike has been my mentor for the best part of 30 years and continues to educate me and introduce me to new great new artists.


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Pump prices in most states have been ticking lower for the past couple weeks but that may be just a temporary treat for drivers. For the week, the national average for regular drops four cents to $2.81 while Oregon's average slips two cents to $3.36.
The average price for a gallon of gas in Hermiston is ranging between $3.17-$3.19.
"AAA expects gas prices will likely start to increase as early as this week ahead of the Nov. 4 Iran sanctions and the midterm elections," says Marie Dodds, public affairs director for AAA Oregon/Idaho. "Peak refinery season is wrapping up, which is helping to push gas prices lower, but declining prices are not expected to be a long-term trend. How high gas prices go will depend on how the markets react to the events in early November."
Oregon is one of 45 states and the District of Colombia where prices have decreased week-over-week. Iowa has the largest weekly decrease (-8 cents). Ohio has the largest weekly increase (+7 cents). This week eight states have averages at or above $3 a gallon, down from 11 a week ago.
Oregon is one of seven states where prices are higher now than a month ago. The national average is seven cents less and the Oregon average is 10 cents more than a month ago. Michigan has the largest monthly decrease (-21 cents). Washington has the largest monthly increase (+13 cents). The West Coast states are the only ones with monthly increases due to a natural gas pipeline rupture in British Columbia, Canada three weeks ago. This forced three Puget Sound refineries to shut production units. Those refineries are now back online, but states on the West Coast saw spikes in gas prices due to the decreased output.
The West Coast continues to have the most expensive gas prices in the nation. Hawaii tops the list for the 21st week in a row with California, Washington, Alaska, Oregon, Nevada and Idaho rounding out the top seven. Oregon is fifth for the second week in a row. On the week, all the prices in the region except for Hawaii are lower.


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Happy sunny Sunday to all you beautiful Galwegians. It's another gorgeous day in G-Town as we spool up for silly season. As usual there are plenty of musical events taking place to keep you entertained, here are just a few of them
The Salthouse Bar Sunday Music Session continues in festive fashion with always sublime Noriana Kennedy and her musical friends bringing the music from 4:30pm. If it's old school Rock and Blues you're looking for then check out Richie Brennan and the Vibe Tribe when they play in E-Bruns bar at 6pm. At 6:30pm you can catch the traditional sounds of Sin a Deir Síwhen they perform downstairs in Monroe's Galway. The SeshWans are back in front of the fire in The Blue Note Galway from around 7pm. Il Vicolo is the favourite location for some winter Jazz by the river with Szymon Misniak bringing the finest guest musicians! Music from around 7:30pm.
Stephen James Smith & Enda Reilly take to the stage in the Roisin Dubh for what promises to be a incredible evening of new Irish poetry. Essential viewing. Doors 8pm
At 8:30pm you can catch Tuam legend Mouse McHugh performing all your favourites downstairs on Monroe's Galway. At around the same time doors open upstairs for the Open Mic Night at Róisín Dubh with master of ceremonies Steven Sharpe keeping order! 9pm is when Greg Cotter and Mary Flanagan take to the stage in the Crane Bar for their awesome Country/Folk session. At 9:30pm Jade Reynolds and the gentlemen of the Rollover will be soothing your ears in Massimo Bar Galway.
If you happen to be across the river then be sure to drop into Tigh Neachtain and support Sunday Night Music – The Timber Tramps as they come back together for one night only to rattle your socks & shine a light on Haiti. Music from 9pm. The always smiling Turlock Holmes and his exuberant musical friends from Pyramid will be hitting the stage in the Quays Bar Galway at 10:15pm. Bass virtuoso Karl Clews gets all freaky with the Superfreaks in the Dail Bar from 10:30pm and we will be hitting the stage in the Róisín Dubh for 12 midnight!
It's a slightly worse for wear group of musicians joining me tonight after an evening of making the amazing Diana Van de Kamp feel truly welcome into her new and wide-spread Irish family! Joining me tonight for some musical salve… Bushkanga Rilla on drums. Mark Sullivan on guitar and Banagher blues. Eoin Mccann on bass. Maciek Zaworonek on harmonica. Barbara Vulso on vocals. Gabo GD on flutes. Bertrand Huvé on sax and Eanna Ryder on keys.
So there you have it folks. Another super busy day of music in Galway and as usual, if you guys make it to the end of the night we would only love to have the pleasure of your company in the back room of the Róisín Dubhwhere we will be doing our best to keep Monday at bay
Doors: 11:30pm
There will be singing.
There will be dancing.


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Forex trading is one of the best methods to generate income for people with ambition to increase wealth. You can start with a small amount of money and a good analysis, you can easily raise capital invested a large sum of money.
There are basically two ways to change operations on their own. One is to become a broker and personally negotiate deep systems using graphical analysis and take time. The second way is much easier and even more effective, using a trading system as a robot or programs for it.
Forex trading can be done with what is known as a robot, which is made specifically to trade in the stock market and foreign exchange, is usually established if certain criteria are fulfilled and uses signals that get into the program and you have the ability to adjust technical parameters for execution.The parameters determine whether the robot will buy or sell at specific points on the climbs or descents.
There are many benefits to be gained by the use of forex robot. This provides activities all day without fail, compared with the human in need of a break, the software can operate continuously without being aware of it at all times.
It is good, and automatic. You can drink coffee and eat breakfast while forex robot earns and multiply your investment. No need of calculations, the robot does it all, just set the parameters and let it do the work alone, you have to analyze each time, a single click is sufficient, and remove headaches and loss of money.
Note that there is a risk you have to take by using this program the best results are traded, what you get is simply the most effective way to make money with forex currency trading.
The disadvantages of this is that following parameters you give them, they have the ability to say something you did not order it at the time, by far a person who has a sense and intuits what can happen. The robot can not learn from experiences and has no brain. Just follow a certain path and stiff so you have commanded.
Here are some tips on how to be successful with the use of these programs (robot) at forex
1 be clear about the basics of Forex and Forex robot operation, knowledge is the best weapon in any company and should treat your trading as a successful business, understand how the forex market works and how your robot works for such market.
– How many operations have the ability to perform the robot in a week?
– How many profitable trades can have?
– What form of action is, what type of trade?
Perform these questions wholesaler robot and ask all things that make you important.
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be when he needs their help and can provide good support.
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Further Information
2017 AGM paperwork now available
The paperwork for the 2017 AGM is now available in the 'committee' section of the members only area of the website.
My appologies for the lateness of this paperwork, but events have been conspiring against me.
Latest News
S7 Events section added
The S7 Events section has been added
Exactoscale now sold by S7 stores
Exactoscale 7mm chairs and fishplates now available
Mark becomes Tickenham
S7 Autumn meeting in Tickenham 2018


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Unblock The Blocks
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"Unblock The Blocks" is a logical game.
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The "Solution"-button is available for all tasks(puzzles).
"Unblock The Blocks" is a very exciting puzzle game.
There are 6 groups of dimension (5x5, 6x6, 7x7, 8x8, 9x9, 10x10).
Each group has 5 categories of difficulty.
Each categorie has 200 levels.
There are total (6x5x200) = 6000 tasks.
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We sold our house this week and have now moved into an apartment not far from where our new house will be built one day. Lol!
So after being alarmed at not hearing from NVR Mortgage for weeks and weeks and then getting a strange message from our new Loan Processor, I did contact my Sales Representative and she got to work. And this week we got our approval from NVR Mortgage. Yeah! But I also learned that our house will not break ground until the end of September or early October and we will close in December or early January. So we will be moving again in 6 months, but this time in the dead of winter. That was not so great news.
But this was a big week for us. We are back in the burbs and one step closer to getting our new house.


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Name: Robert jordan ebook
File size: 910mb
Language: English
Rating: 4/10
13 Feb The offering for February is a big one: Robert Jordan's The Eye of the World , the first volume in his epic Wheel of Time fantasy series!. Results 1 - 16 of The Wheel of Time Companion: The People, Places, and History of the Bestselling Series. Nov 3, | Kindle eBook. by Robert Jordan. Robert Jordan was born in in Charleston, South Carolina. He taught himself to read when he was four with the incidental aid of a twelve-years-older.
16 Feb If you like epic fantasy novels, Robert Jordan's The Eye of the World is the start to one of the most epic fantasy series ever written, and for the. Results 1 - 16 of Nothing expands the mind and takes you on a journey like a well-told story. As any avid reader knows, books are more than just means of. 9 Jan Once you get there, you can know you're reading Robert Jordan's words, . Question: Why a delayed ebook release for A Memory of Light?.
Written by Robert Jordan and completed posthumously by Brandon Sanderson, The Wheel of Time is one of the greatest epics of fantasy ebook, pages. 16 Sep Amazon is currently running a special on The Eye of the World ebook for the Kindle for only $ Originally released in , The Eye of the. (eBook) Robert Jordan - Wheel of Time (Historical) - The Strike at Shayol Ghul - Download as .rtf), PDF File .pdf), Text File .txt) or read online. 6 Sep Robert Jordan is the author of eight New York Times bestselling books of fantasy. Snow will be available in multiple eBook platforms and the. 8 Sep Robert Jordan and Brandon Sanderson's THE GATHERING STORM– Chapter One, Prologue, eBooks/ Art, and STORM LEADERS. September.


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Wall Colors For Kitchen With Oak Cabinets - It's a fact that our moods are influenced by color. That is why it is vital that you choose the correct combination of colors for the kitchen to ensure it is a pleasant place to be. Soft yellows and blues could make people feel more joyful, and colors of earth and green tones discovered in nature- warm toasty browns and sandstone can provide a feeling of tranquility and calm.
Shade can also be used to highlight the buildings of your kitchen, or hide it, depending on your inclinations. You may also use colors to determine how it relates to the other adjoining rooms of your home, and how large or little your kitchen will feel.
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Early 914 steering wheel has the identical flat hub to a 911 wheel. Same size as used for 911RS. No modification or drilling needed. Bolt on and go. Wheel has light patina. Leather is rough in spots and stained in others. Spokes are original paint but also have some blemishes. Stitching is 99% there. Looks great in some cars, wont look good if you want new. $395 + 20 ship. Email - firstname.lastname@example.com to purchase. 914 347 805 10, 71 3
I also have the same style wheel without leather, just the rubber rim, for $300 + ship. Its a great base for a RS style thick grip wheel.


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Travis Wm. Bennett: Obama fulfilling his promises
Blue River
In response to Don Severe's letter of 28 March, I must say that believing the last two presidents since JFK that knew how to speak are con men is somehow incredulous. Just because they got their ideas across doesn't make them any more of a con man than the president in the middle (George W. Bush). While certainly not very articulate, Mr. Bush managed to con the Congress into letting him start an unnecessary and immoral war (assuming any war can be moral!). He also managed to con the Republican Party (and the electorate in 2004) that he was Christian, compassionate and conservative. With the possible exception of AIDS relief to Africa, there was no demonstration of any of the above for most of eight years in office.
Mr. Obama has told us in no uncertain terms that he would do things differently, and he has. We trusted his word and voted for him in an overwhelming fashion. I don't see how anyone could call his campaign or presidency a con; a significant majority of Americans were ready for a change, and he is doing his best to deliver.
Start a dialogue, stay on topic and be civil.
If you don't follow the rules, your comment may be deleted.


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Provided by Carl J. Spallino Funeral Home
|Posted by: Frank and Robbie Skrypak
Wed January 23, 2019
|Jeanie, Jack and all family of Terri. You have our deepest condolences in the loss of your Mother/Mother-in-law, Grandmother, Sister-in-law and Aunt.
Her neighbors will feel her loss.
May she Rest In Peace,
Frank and Robbie Skrypak


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Last year I returned to daily professional writing upon retirement. As a result, two books are now published! The first is brand new! The second is in second edition. Now I spend time working within interesting writing projects. There is now plenty of time to inspire myself and others! Hope you enjoy. Each provide opportunities for personal growth. The Googol of Wishes adventure is written to inspire grandparents, parents and kids alike. Learn to imagine your finest world!
Listen to our Audio Book Preview: "Getting To Happy: Learning to Read Emotional Messages." Spend the day with this happy coach and author. Practice fun!
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When you own or use as many computers as I have, probability says that eventually you'll have problems with one. Usually I'm the one called in by a family member when their computer is acting up, and 99% of the time its a problem with ill-behaved software that's corrupted the registry, or too many inadvertent malware downloads bogging the system down. The best solution at those times is the computer version of a frontal lobotomy; buy a new hard drive, install a clean copy of the operating system on the new hard drive, and use the old hard drive as a data drive. The good news under those circumstances is that the computer then performs as fast and nimbly as it did when it was new – sometimes better. The bad news under those circumstances is that the machine effectively is a new machine, so all their software needs to be re-installed.
This time I was the one with the problem, and it wasn't anything that a new hard drive and clean operating system would fix (though I did – and do – have the new hard drive standing by, with a new copy of the new Windows 7 operating system, but I was waiting until after the holidays to conduct the self-inflicted frontal lobotomy on this machine).
My main workstation, the 2-year-old PowerSpec E360 tower downstairs in my subterranean home office (with Intel Core 2 Quad Processors, enhanced with 4 GB of memory and 2 TB of disk space, a Hauppauge HVR-1600 NTSC/ATSC/FM tuner, and other nifty features) died. We got home from Maine Sunday the 6th and it was dead as a doornail. I usually leave my machines on 24×7 with the monitor shut off (I have the house server, a Linux box, and this workstation all tied together with a KVM to save desk space), but this weekend, for some reason, I decided to power down the Linux box and my workstation (leaving the house server up for the remaining inhabitant while we were away).
The symptoms were odd; I hit the power switch and it started booting up (fans spun up, hard drives spun up, monitor flashed through the bootstrap process), and got all the way to Windows Vista login when it abruptly died as if someone had pulled the plug. I knew it wasn't a power failure since the room lights were still on, and the workstation has its own UPS. After thinking for a few seconds about what had just happened, I tried again and hit the power switch. This time it booted up all the way, I logged in, and did my email and a few other mundane tasks (checked some web sites, paid some bills, etc.). Then I decided to push the envelope and shut it down again, to see if the problems would re-occur. It didn't even get through the P.O.S.T. before it died. After that, it's been catatonic ever since; no fans spinning up, no disks spinning up, no life showing from the monitor.
I assumed the culprit was the power supply, since neither the cooling fans nor the hard disks would spin up, the only thing that happened was the little blue power light on the front lit up. Thank goodness for my MOZY backups! I've been pulling files down from my online archive to use on my laptop as I need them. LESSON #1 – implement a disaster recovery plan – BACK UP YOUR DATA!!
When I pulled the side panel off the machine, disconnected all the power supply cables (two to the motherboard, one to each of three hard drives and a DVD writer, ones to each of the cooling fans), and plugged in my power supply tester, it tested as working perfectly – NOT what I had expected! So either it wasn't the power supply, or my power supply tester was giving me false positive readings. I decided to assume that the tester was working properly, which meant it was something else. My toolbox of hardware diagnostic tools is fairly sparse, so I was facing a series of blind part-swapping exercises in a trial-and-error attempt to stumble upon the offending item.
I wussed out and took the machine to MicroCenter (where it came from) Monday night for repair; $70 for a full diagnostic on a bench with real test equipment. This time of year I don't have the time or cash to to do the trial-and-error parts replacement firedrill until I find out what part was the culprit. Turns out it wasn't the power supply, it was the motherboard! A new one will cost $80 (pro-rated) with another $60 to install it (moving my 4 GB of memory, CPUs, and expansion boards over to the new motherboard as well). Without sophisticated test equipment I would've spent three times that on replacement parts that didn't need replacing before I stumbled upon the motherboard as the source of the problem.
Once it's back (should take a week to get the replacement motherboard and a few hours to move the components over) and has been verified as healthy with a month or so of error-free operation, I'll add the new hard drive and install 64-bit Windows 7 Professional on it. I've got 32-bit Windows 7 Pro running on my 7-year-old IBM Thinkpad laptop and it's great. You can't migrate Windows XP to Windows 7, you have to do a clean install, so I bit the bullet and bought a new 250 GB laptop hard drive to replace the 60 GB drive that came with the machine. Then after the install I added another GB of memory to the GB already in it, and it's a humming little laptop.
Still anxiously waiting for my workstation to come home from the hospital, though!


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Global Geomembranes Market Overview:
The growing development of infrastructure around the world, mainly associated with the construction of new roads, promotes the geomembrane market. Geomembranes are used gradually in road construction activities. A geomembrane acts as a barrier to the movement of fluids, water and other soluble materials, and is manufactured using synthetic polymers that are impervious in nature. Geomembranes have been more effective in terms of permeability than other traditional products, namely, asphalt, concrete and compacted clay. Geomembranes are also used to strengthen the weak soil that joined them, increasing the useful life of the roads.
Geomembranes are flat sheets made of less permeable or waterproof materials. Geomembranes are served in different sizes and qualities in the world market. They are widely used in water containment and base coat applications. Geomembranes are generally made of high-density polyethylene (HDPE) or linear low-density polyethylene (LDPE). On the other hand, geomembranes with their extended functionality, such as their exceptional ability to withstand high temperatures, weather resistance, chemical resistance, low permeability, UV resistance, tear and perforation, are used in many applications.
Browse report brief and TOC @ https://www.researchcosmos.com/reports/world-geomembranes-market-to-2022-global-analysis-and-forecasts/9373616
Global Geomembranes Market Drivers and Restraints:
The global geomembrane market is expected to grow rapidly in the near future. Greater knowledge of water conservation in the construction and manufacturing sectors worldwide should promote the importance of geomembranes as a containment solution in the coming years. In addition, it is expected that the increase in technological developments, such as the new arc equipment in which the geomembrane is used for leak detection by TRI Environmental, creating new opportunities for geomembrane manufacturers in the near future. In addition, Conley has incorporated TPO in geomembranes to cover photovoltaic panels. As a result, the use of TPO-based products in photovoltaic solar panels should force other consumers in the solar industry to include geosynthetic materials in the coming years. The growing practice of geosynthetic clay lining in landfills and coating systems is likely to pose a substitute threat to the geomembrane market. In addition, it is proposed to increase the demand for bituminous membranes in landfills and sealing to reduce geomembrane applications during the forecast period.
Request free sample @ https://www.researchcosmos.com/request/world-geomembranes-market-to-2022-global-analysis-and-fore/9373616
Geographic Segmentation
North America is expected to dominate the global geomembrane market due to the growing demand for geomembranes in the mining and waste management industries. It is estimated that Western Europe is the estimated to follow North America due to the high consumption of geomembranes in the local market. In addition, strict government regulation around the world for environmental protection should also boost demand for geomembranes in a wide range of applications like oil and gas, energy, coal ash containment, civil, agricultural, concrete, athletic confinement, spills or secondary and industrial applications. In addition, APEJ and MEA are also expected to show moderate growth in the market.
Inquire Before Buying @ https://www.researchcosmos.com/inquire/world-geomembranes-market-to-2022-global-analysis-and-fore/9373616
Key Players In Global Geomembranes Market
The main players in the global geomembrane market are GSE Environmental, LLC (USA), Atarfil SL (Spain), Carlisle SynTec Systems (USA), Solmax International, Inc. (Canada), Plastika Kritis SA (USA). Greece), Officine Maccaferri SpA (Italy). ), Agru America, Inc. (USA), NAUE GmbH & Co. KG (Germany), Colorado Lining International, Inc. (USA), Firestone Building Products Company, LLC (USA), Etc.
About Us:
Research Cosmos is a provider of standard and customized market research, business intelligence and consulting services across more than 100 domains in different industries of the world. We host the trending market reports of the world's top-notch publishing companies, offering services to a wide range of customers from students to fortune 500 companies and discloses the hidden opportunities in every leading industry of the world.
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Leander High (LHS) Class of 1952 Alumni List
Help us Update the Leander High 1952 Class List by adding any names missing from the list below:
Registered Alumni
1952 Class List
Know Leander High Class of 1952 graduates that are NOT on this List? Help us Update the 1952 Class List by adding missing names.
The Greatest Show on Earth was the top movie in theaters in 1952, with a lifetime gross of over $36,000,000. Among TV shows in 1952, Arthur Godfrey's Talent Scouts was the most popular of the year.
Reunions and Leander High School News
More 1952 alumni from Leander HS have posted profiles on Classmates.com®. Click here to register for free at Classmates.com® and view other 1952 alumni.
The Leander High class of '52 alumni are listed below. These are former students from LHS in Leander, TX who graduated in 1952. Alumni listings below have either been searched for or they registered as members of this directory.
If you are an alumn of Leander High you should register now in the alumni directory. We also encourage you to interact with the rest of the alumni on this site. You can find old friends, share photos and stay updated on the latest school news.


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Linda Kurschinske Dolecki
Oil City High Class of 1969
Personal Info
- Name:
- Linda Dolecki
- Maiden Name:
- Kurschinske
- Graduation Year:
- 1969
Register to view Current location, Gender, Birthday, Relationship Status, Children, Employer, Military, College, and Interests
Memories of Linda Kurschinske Dolecki
What I'm doing now:Register to see what Linda Kurschinske Dolecki is up to now!
Linda Kurschinske Dolecki's Latest Activity:
|- Registered as a OCHS Class of 1969 alumni. 2009


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Amelie 'Collector's Cabinet' Wall Shelves (Large)Buy Now at Amazon
This large display cabinet comes with a wall fixing kit and mounts on the wall, its ideal for boys and girls bedrooms where the three large shelves inside can be used for storing and displaying photographs, school, certificates, newspaper cuttings, awards, trophies or just anything that your little boy or girl would like to collect and save.
The display cabinet has an elegant bevelled frame and is made from wood with a white coloured finish. The front door of the cabinet has safety glass inside so its safe for children's bedrooms and the door has a latch to close. You can mount the wall cabinet in the bedroom or the lounge or sitting room either at a height that the children can reach and open the display cabinet or higher than they can reach so they can see the contents of the cabinet but not touch.


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Godrej Sector 85 Gurgaon- Prime Address of all the Years
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Got another batch of rumors for you. This time we step back from the Grim Darkness of the distant future and instead approach the grim darkness of the Mortal Realms. Peering ahead through the salt-addled haze advised for rumors, we find: goblins.
The rumormill keeps on turning, Proud Mary keeps on burning. Here's what's rolling down the river:
Greenskins, mostly. And Grots, specifically. According to another old school Rumormiller, we can all except a diminutive green tide later this year as the Grots get their own Battletome. Building on the burgeoning narrative outlined in Malign Portents, we'll apparently be seeing more Moonclan. Which I suppose makes sense: after all, the Soul Wars brought us Nighthaunts and a new chamber of Stormcasts, all inspired by the Harbingers that appeared in Malign Portents.
Harbingers, for those of you who never picked up Malign Portents, were four special characters, one for each Grand Alliance that could do special things if you were playing a Malign Portents matched play game. And for the most part they've kinda fallen a little by the wayside, except if you look at what's been releasing lately, it looks like Malign Portents has outlined the next couple of big releases. After all, we got the new Sacrosanct Chamber, heralded by Lord Ordinator Vorrus Starstrike, and also the Nighthaunts who we saw in the form of the Knight of Shrouds.
That leaves two characters left: the Moonclan, and the Darkoath. For now, we're focusing on the Moonclan, who apparently will be riding at the head of a horde of grots. According to rumors, we'll be seeing a bunch of new kits to replace old models that are being phased out, including new Spider Riders, who we've needed for a while.
Following the example of the Fungoid Cave Shaman, there are rumors of Mushroom enhanced goblins, so maybe these will be another line of casters–kinda like we've seen more Ordinators show up. Or maybe they'll be mushroom-addled and have random abilities in an almost squig-like fashion. Who knows.
A lot of models are rumored to get an update, including generic Grots and Snotlings, which if you look at the old models, sorely needed.
Finally there are rumors of Grot-themed Endless Spells. Maybe this is where that charging, flaming Bull has come from. But that seems a little more Chaos-y to me. So watch this space for more news and rumors as they develop. We'll see soon enough–in the meantime, keep your spice rack well stocked, because the rumors must flow.
The one thing you can say about that Cave Shaman is that he's the life of the party. A real fungi.


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the option of management. Wir verwenden Cookies, um Inhalte zu personalisieren, Werbeanzeigen maßzuschneidern und zu messen sowie die Sicherheit unserer Nutzer zu erhöhen. Services, tickets, online: Visa and MasterCard accepted for online ticket purchases. BOX office: Tickets may be purchased in person.m. Must present ID in person at time of purchase (no online or phone sales). Check Santa Clarita Guide's Calendar of Events for details.
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I am trying out a random vibration study in a beam. It is a beam fixed on both ends and a remote mass is defined on mounted hole on the beam.
Input is the PSD curve base excitation. I would like to find the reaction force on those highlighted points bu the value is zero.
Also on the remote mass mounted hole the reaction value is zero for all the natural frequency values?
You can have reaction forces only in the constrained nodes. If you want reaction forces in those nodes, you have to fix them in space so that the displacement is zero.
Retrieving data ...


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BENGALURU: ISRO and its commercial wing Antrix today said they are willing to outsource manufacturing of Polar Satellite Launch Vehicle (PSLV) and the Small Satellite Launch Vehicle (SSLV) so that the space agency could focus on the proposed human space programme.
The Indian Space Research Organisation held discussions a week ago with a consortium of industries regarding PSLV industrialisation with the objective of easing ISRO to focus on human space programme and researchand development activities.
"Definitely, in my opinion, in the ISRO activity, industry is going to play a major role. If only the industry is taking the load, ISRO can work out on human space programme and research and development activities," ISRO chairman K Sivan said.
He said the industry should help in manufacturing PSLV as well as SSLV, adding ISRO had already worked out a model.
With this model in a year, the PSLV was supposed to be produced by the industry. "That is our ambition and target," Sivan said. (AGENCIES)


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Farrah Storr is the editor of 'Cosmopolitan' (UK) and author of 'The Discomfort Zone: How To Get What You Want by Living Fearlessly' Farrah Storr is an authoritative name in women's journalism. In 2017, she was named one of the thousand most powerful people in the UK. She was one of only seven BAME women… Continue Reading ➤
Why Putin was right to annex Crimea
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Lone-operator Terrorism: Tackling a Growing Threat
For 17 years, the USA lived in fear of the "Unabomber", Theodore Kacynski, who sent package bombs to unsuspecting victims. Over the course of these 17 years, the FBI acquired and analysed 82 million records, 9,000 event photographs and 12,000 event documents, but was unable to bring Kacynski to justice. It was eventually his brother… Continue Reading ➤


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Centro Hotel Mondial, Munich
- Free WiFi
- Collect nights
What's around Centro Hotel Mondial, Munich
- In Munich City Centre
- Viktualienmarkt - 13 min walk
- Marienplatz - 13 min walk
- Theresienwiese - 14 min walk
- Hofbrauhaus - 18 min walk
- Deutsches Museum - 20 min walk
- English Garden - 42 min walk
- Sendlinger Tor - 4 min walk
Getting around
- Munich (MUC-Franz Josef Strauss Intl.) - 37 min drive
- Munich Central Station - 11 min walk
- Munich Harras Station - 4 min drive
- Munich Ost Station - 5 min drive
- Sendlinger Gate U-Bahn - 4 min walk
- Karlsplatz (Stachus) Station - 8 min walk
- Goetheplatz U-Bahn - 10 min walk
- Self parking (surcharge)
At a glance
Key facts
Hotel size
- This hotel has 57 rooms
- This hotel is arranged over 6 floors
- Check-in time 2 PM-midnight
- Check-out time is 11 AM
Required at check-in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- No cribs (infant beds)
- No pets or service animals allowed
- Free WiFi in public areas
- Free WiFi in rooms
- Self parking *
Other information
- Smoke-free property
In the hotel
- Full breakfast daily (surcharge)
- Computer station
- 24-hour front desk
- Tours/ticket assistance
- Free newspapers in lobby
- Luggage storage
- Multilingual staff
- Number of buildings/towers - 1
- Elevator/lift
- English
- German
In the room
- Iron/ironing board (on request)
- Private bathroom
- Bathtub or shower
- Free toiletries
- Hair dryer
- Flat-screen TV
- Desk
- Free WiFi
- Daily housekeeping
Centro Hotel Mondial, Munich's small print
Also known as
- Bristol Munich
- Hotel Bristol Munich
- Centro Hotel Mondial Munich
- Centro Mondial Munich
- Centro Mondial
Parking height restrictions apply.
Optional extras
Self parking costs EUR 17 per day
Full breakfast is offered for an extra charge of EUR 8.90 for adults and EUR 8.90 for children (approximately)
We have included all charges provided to us by this property. However, charges can vary, for example, based on length of stay or the unit you book.
Didn't find the right property?Continue searching for Munich
Recent reviews
Good 7.2 From 331 reviews
Hotel is just outside the Sendinger Tor U bahn exit. Room is relatively new. Sheets and towels clean. We booked a triple room, which is really an additional bed add on to the twin room, thus not much walking space. A bottle of water was given on the first day, after that no more replenishment even though housekeeping was done daily. Walking distance to attractions, e.g. Marienplatz, Karlsplatz, Christmas markets. Breakfast buffet is payable at Euro 8.99, if you wish to. Can't comment as we did not eat. Cons: No kettle There's a mild stench in the toilet. Heater is not warm enough in cold days. Reception staff are not friendly. Check in time is strict, even though we were just 20 mins before time.
Didn't reply to email
Close to Oktoberfest and sites in Munich, but small rooms, uncomfortable beds, noisy area, and disorganized check in.
It was a fine hotel. Good for the price good location near the city center. No air conditioning in the rooms.
Great hotel and very clean with friendly staff. If you're gay, the location is perfect! Right where you want to be next to the subway and gay bars. Just down the road from the train station (15 min walk) and there is a major subway station with all the connections you need like 20 feet from the hotel front door. Beautiful fountain on the corner next to the hotel, safe clean gay area and Munich is a great city! I will definitely stay here again.
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prick up ears
prick up ears
also prick ears up
- to start paying attention
- to begin the process of listening (to someone) intently
Example Sentences
- The teacher asked her student to prick up his ears when he kept on disturbing the class with silly jokes.
- I tell my husband to prick up his ears when taking down the grocery details for the month else he tends to forget to buy certain important things.
- The sales staff was asked to prick up his ears when he could not suggest anything that the client considered buying.
- To not prick up your ears when you are working at a call center would mean complete disaster for your career. It is among the most basic requirements of a calling job.
The phrase originates from the animal world where pets like horses and dogs are able to fine tune their hearing by picking their ears up. The first literary use of the phrase was in the year 1626 in the work titled "Essays – On Fame" by Francis Bacon. The phrase is also the title of an English play which has been based on the playwright Joe Orton's life which enumerated the person's scandalous reputation and was not entirely connected with the phrase's meaning.
- pick up your ears
- cock an ear
pipe down ❯❮ suited and booted
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Idiom of the Day
bee in bonnet
have a bee in bonnet Meaning be possessed with one thought or scheme to be obsessed with an idea Example Sentences The man has a real bee in ... Read on