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Designer proteins for new bio-tech applications
An EU-funded project has developed a technique to design and synthesise highly stable proteins. The approach enables proteins to be made from scratch, tailored to carry out specific tasks for advances in fields such as biomedicine and biotech - boosting Europe's competitiveness.
© Gustav Oberdorfer, Possu Huang, 2014
Protein molecules are becoming increasingly essential for use in the fields of biomedicine and biotechnology. In medicine, proteins are replacing small molecule drugs, providing more effective and targeted treatment.
They are also being used as biocatalysts in industrial processes, reducing the environmental impact of advances in biotechnology. However, producing bespoke proteins with functionality and structure that is tailored for a specific application is no easy task.
The EU-funded ASR-COMPENZDES project has developed a new method to design and produce proteins. This takes advantage of sophisticated computational modelling tools to make new helical protein structures with unprecedented stability.
The newly designed proteins are stable at temperatures above 95 °C and in strong denaturing conditions. This makes their applications wider ranging than traditionally developed proteins, or those found in nature.
'We have developed a robust technology that can build alpha-helical protein structures from scratch,' says Gustav Oberdorfer of the University of Graz in Austria. 'We can design a protein and pack its core really well, so that it becomes very stable. We can then try to adjust its functionality so it can be used in biomedicine or biotechnology as a drug, enzyme or nanomaterial.'
Crick's equations
To design proteins, ASR-COMPENZDES combined equations developed in 1953 by Francis Crick, UK molecular biologist, biophysicist and neuroscientist, with modern computational methods. The equations accurately describe helical protein structures and, with the aid of today's computing advances, can determine the amino-acid sequence needed to create a desired protein.
This bottom-up innovative approach can produce proteins with specified structures and functions. With the amino-acid sequence laid out, a DNA construct can be created that encodes for the designed protein to be produced.
Proteins for biomedicine and biotechnology
Proteins and antibodies used in biomedicine are often relatively unstable and must be transported and stored under the correct conditions. With the results of the ASR-COMPENZDES project, these issues are not a concern, and designed medications and treatments which could result from this new technique have the potential to reach more patients worldwide. Highly stable proteins can also be used more widely in medical diagnostics, bioindustry and biotechnology applications.
'These highly stable proteins can be designed to suit many applications,' says Oberdorfer, whose research was funded through the EU's Marie Skłodowska-Curie actions programme.
'We hope they will play a key role as biocatalysts and help reduce reliance on organic solvents and harsh reaction conditions. This will help make industrial processes greener and more environmentally friendly.'
The project led to a number of publications in international journals and the involvement of students both in Europe and the US. The results have been presented at international conferences. Some aspects to further fine-tune the technique to create bespoke proteins are now being supported with additional research grants.
Project details
See also


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Two houses targeted by thieves in the Lakelands area of Naas
Gardaí in Naas are investigating two burglaries which occurred in the Lakelands area of Naas on Sunday October 8 last.
The burglaries happened between the hours of 2:30-4:25pm, while the occupants were out.
Rear windows were smashed in the incidents.
Anyone with information is asked to contact Naas Gardaí on (045) 884 300.

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Have you ever wondered if God has seen the bad things that have happened to you? This short book, the shortest in the Old Testament, will show you that God sees everything. Watching the Edomites raid Judah and Jerusalem, who are supposed to be family, God's holy justice rises against them and you see that our God is no wimp.
DIVE | deep dive
Rise, let us go against her for battle
god is not a wimp, let him take care of you!
Have you ever been in a situation that was completely unjust and felt like God did not back you. I know this sounds terrible but there have been times in my life that "forgive and turn the other cheek" felt like I was allowing the person to get away with what they had done. It felt so wimpy, so unjust. I wanted justice, vindication, but God called me to forgive. And with gritted teeth and a surrendered heart I forgave.
The more I walk with God the more I realise that God is not a wimp. He is the all-powerful, all-knowing Creator of the heavens and earth. He is the God of mercy and justice, the God of love and righteousness, the God of grace and holiness and he sees everything. He is one of those super mums that has eyes in the back of their head. Even though their head is turned away from you, you can't get away with anything, they see all!
edom, pride & judgement
As we have seen in the dive, the main point that the book of Obadiah is trying to make is that God's people should take courage, and the reason for this is that God still reigns supreme over the universe and all its inhabitants. The hope that God's people are to cling to is that God will take care of us and that the wrongs done unto the house of Judah will be made right.
What struck me as I read this book and as I researched the Edomites was their pride which had brought God's judgement upon them. We see very early in the book that God himself directly opposes Edom and in verses 2-4 we find the basis for God's disapproval and hostility towards Edom: "Behold, I will make you small among the nations, you shall be utterly despised. The pride of your heart has deceived you, you who live in the clefts of the rock, whose dwelling is high, who say in your heart, 'Who will bring me down to the ground?' Though you soar aloft like the eagle, though your nest is set among the stars, thence I will bring you down, says the Lord."
What we see here is that the Edom's pride is the root cause of their impending doom, for their pride deceived them into thinking that they were as secure and out of reach of men. They forgot God and so he declares that they will be brought down by his judgment.


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New year, higher pay: 2019 changes affecting small business: Many small businesses will see their labor costs going up in 2019.
President Trump tells boy that believing in Santa at 7 is 'marginal'
The littlest opioid-exposed children in Ohio are getting some new help.
National search to begin for a new Columbus Police Chief.
Salvation Army chooses not to persecute thieves in the "Spirit of Christmas."
Kevin Spacey posts video online in character as his "House of Cards" role, saying "I'm not going to pay the price for things I didn't do."
Toledo goes crazy for roadside weed decorated for Christmas.


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Orbital Sciences Corporation launch of a Minotaur I Rocket
Orbital Sciences Corporation was scheduled to launch a Minotaur I rocket as part of the NROL-66 (RPP) (USA-225) mission. The launch window for the Government/Top Secret mission was on Sun, Feb 6th, 2011, 7:26 AM EST from Space Launch Complex 8, Vandenberg AFB, CA. The status of the launch was Success. Don't miss this exciting rocket launch!
USA-225, also known as the Rapid Pathfinder Prototype (RPP) and NRO Launch 66 (NROL-66), is an American satellite which was launched in 2011. The satellite is being used to perform technology demonstration and development experiments, including advanced dosimeters to characterize the space environment from a 1,200 kilometer low Earth orbit. It is operated by the United States National Reconnaissance Office.
LocationLaunch Pad Compass (Beta)
Space Launch Complex 8, Vandenberg AFB, CA
Minotaur IOrbital Sciences Corporation
View More Rocket Launches


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Hi ladies, I got a faint line on a pregnancy test last week which progressively got lighter. Pretty sure I'm having a chemical pregnancy. I'm on cd38, 17dpo and AF is 7 days late. Has anyone experienced a CP after TFMR? I'm wondering if it has anything to do with the D&E?
I had TFMR back in December 2015, got a positive test result early March 2016 (tested twice with digital n regular first response) than AF was there a day late heavy and sharp cramps and blood was bright red like never before. My doctor stated it could have been in fact a CP as they are quite common. I did not even know what a CP was until doctor explained it to me. Even though it seemed as if my AF came a day late(cycle is super regular) I was crushed as I thought I was really preggo. A month later I got pregnant again currently 15 weeks hoping this is our rainbow. Oh I too had D&E and do not believe that is what caused the loss. Have you gone to doctor to get blood work?
Yes. Just got my bloodwork back and I was definitely pregnant last week. :(
My hcg level was really low, 18.
I guess I started miscarrying on Sunday when my temperature dropped. Started bleeding 1 week after my period was due. 2 losses in 6 months :(
I'm so sorry you are going through this.
Thanks for the support ladies. Feeling angry for the first time and just want this whole nightmare to be over. I'm sure many of you can relate...


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We had first seen the Crocketts about eight months before when they opened for Stereophonics at the Wolves Civic Hall, and had become firm fans. It was a Christmas show and there – at the bar – was Kelly Jones. The Stereophonics frontman had just finished a UK tour, in arenas. I had seen the NEC show and it lacked the brilliance of the of the Wolverhampton spring affair. Jones spent the evening, chatting, he was friendly and happy, it seemed, to be at a gig. He later got up on stage to perform with his friends (from memory a cover of "My Girl" but let me off it was nearly 13 years ago) and it was a great night.
I tell this story for two reasons. First, because that was the last time we saw the Stereophonics in any guise. Them having drifted off into ever more bland and indie areas and me staying with rock and metal, and second because it proves that Jones had absolutely none of the "rock star" about him. It is this attitude, you suspect, that means that, for this tour they choose to do much smaller venues. Lets be honest, if they wanted to they could be at the NIA up the road if they wanted. The fact that they are in a 1400 capacity place like the Academy speaks volumes for their ethos.
It was this that led me to buy a ticket. Here was a chance to watch a band we used to like in the habitat we used to watch them in. And given that they start with "Bartender And The Thief " the lead single from "…Cocktails" it truly is like being transported back to my mid-twenties. This is followed by "A Thousand Trees" from their quite marvelous debut record and it is remarkable how quickly I am remembering these songs.
This is not a greatest hits set though. This pre Christmas jaunt is to try out tracks from a forthcoming record and also to break in new drummer Jamie Morrison. The latter is, in our opinion much more successful than the former. Morrison is good. Two of the new tunes not so, given that they are occupying the same middle of the road territory that much of their later output has. In fact, the inclusion of "Mr Writer" halfway through the set is rather apt, given that song is the exact moment when I stopped loving the band. It remains to this day, dull and whiny.
What you can't do is argue with trio of classics that the band end the main set with. "More Life In A Tramps Vest," "Just Looking" and "Local Boy In The Photograph" are just fabulous. As, to be fair, is the encore. The third new track "Violins and Tambourines" is a slow-burning epic that echoes Marillion and "Traffic" is a piece of quirky brilliance."
It is last song, "Dakota" though, that really sums up the night neatly. It is a decent song, yes. But not to these ears anywhere near what was on the first couple of records. The crowd, however, goes ballistic. And good luck to them. All this shows to RTM though, is that The 'Phonics aren't our band anymore. They became global when we weren't looking, while we were still at The Flapper looking for excitement.
A good gig, but one which will be gig of the year to most the audience, even if not to me.


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https://trustedadvisor.com/public/ 300 400 Charles H. Green http://trustedadvisor.com/public/trusted_advisor1.png Charles H. Green2010-08-16 10:00:002010-08-16 10:00:00Golden Oldie No. 1: Operating Transparently
Golden Oldie No. 1: Operating Transparently
We're taking a little break and going off-blog-grid for a few days:
To paraphrase Mark Twain: Persons attempting to find a motive in this action will be prosecuted; persons attempting to find a moral in it will be banished; persons attempting to find a plot in it will be shot.
But to keep the withdrawal symptoms to a minimum, we shall be reprinting a few from the archives.
Today's golden oldie is: Operating Transparently


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What is the Economic Outlook for Latin America?
Economic recovery in Latin America and the Caribbean (LAC) has lost some momentum. Near-term growth prospects in LAC are weighed down by tighter global financial conditions and an intensification in trade tensions. The moderating recovery is also underpinned by divergent growth outcomes across the region, as the impact of external headwinds has been amplified by country-specific vulnerabilities, especially in the largest economies. There are bright spots to the outlook, however. Despite the slowdown in regional economic activity, private investment is showing signs of life. Nevertheless, medium-term growth remains stuck in low gear, slowing progress in catching up to income levels in advanced economies.
The Inter-American Dialogue is pleased to welcome Krishna Srinivasan, Deputy Director of the Western Hemisphere Department, to review the IMF's latest economic outlook for the LAC region. His presentation will cover key challenges going forward to help the region's policymakers boost productive capacity and raise investment and saving rates in order to build strong, durable, and inclusive growth over the medium term. A brief commentary and a wide-ranging discussion will follow the presentation.
Copies of the IMF Western Hemisphere Regional Economic Outlook report will be available.
Follow this event on Twitter at #LATAMEconomy and @The_Dialogue.
Krishna Srinivasan
Deputy Director of the Western Hemisphere Department, IMF
Michael Shifter
President, Inter-American Dialogue (@MichaelShifter)


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Steve Harvey's daughter Lori Harvey was caught "flirting" with Jay Z yesterday, as Jay's spouse Beyonce was away internet hosting an occasion for his or her RocNation firm.
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Lori made certain that she bumped into Beyonce's husband as he was working the room.
And Lori was brazenly flirting with the very married pare and mogul.
Fortunate for Beyonce, it does not seem that Jay Z was falling for Lori's attraction offensive. At one level within the dialog, it seems that Jay Z tells Lori Harvey "'I am married." And in response, it seem that Lori says to Jay, "I do know however like."
The video lower off, so it is inconceivable to know for sure how the dialog ended.
Watch the video and skim each of their lips:
Jay Z and Beyonce have been the topic of dishonest rumors earlier than. Beyonce brazenly admitted that Jay Z cheated on her a number of instances, and that she nearly left him.
Jay has since gone to counseling, and claims that he plans to NEVER cheat on Beyonce once more.
We simply marvel if Jay has the energy to withstand that recreation that Lori Harvey was spitting in his ear although . . .


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Tract 11 Cherokee Trail is a $25,000 property on a 4.12 acre lot located in Elk Mountain, WY.
4.120 acres in the Cherokee Trail subdivision, near Elk Mountain, WY. Located on the north end of Pennock Mountain. Great views. Perfect weekend or summer retreat location. No utilities in place; well, septic, generator would be the responsibility of the buyer. Access to the national forest by subdivision owners is via Tract 36.
|Exterior Features
Covenants - Current
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Google fined $57M after France finds violations of new European Union privacy lawGoogle has kicked off 2019 by getting hit with yet another multimillion-dollar fine from a European regulator. However, the GDPR provides that the consent is " specific " only if it is given distinctly for each goal .
Herbert concedes Dubai Desert Classic leadI just felt uncomfortable, unfortunately, on a lot of them, and consequently my ball-striking is just not my best. Three-time victor Ernie Els (70) and Matt Wallace (69) are two shots further back after three rounds.
Trump adviser Roger Stone charged in Mueller inquiryJackie Speier told MSNBC's Ari Melber, Stone's arrest could just be one of many dominoes still to fall. Roger Stone is a political lobbyist and an ally of President Donald Trump .
Jaguars announce London game opponent for 2019 seasonThe other two games will pit the Chicago Bears against the Oakland Raiders and Cincinnati Bengals against the Los Angeles Rams . The Bengals first played in London in the 2016 season, hosting the Washington Redskins at Wembley Stadium.


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Summary of Altruism, Euthanasia and Doctors
From the olden days when doctors were practicing medicine inside the traditional way, there was clearly a principal bond established from a doctor as well as a patient, and euthanasia do not possess been an issue. A doctor would have treated the sufferer in the humane and compassionate way. Now however, on this consumer society where you can find consumer laws and litigation to cope with, our value system changed. It has destroyed this true doctor-patient relationship. The laws have inculcated doctors into treating patients as consumers and as a result the two doctors and the patients have become the losers. The doctors cannot treat patients as they must be treated as well as the patients is not treated as they may wish to be treated. They are kept alive under all circumstances regardless of what quality lifestyle may be waiting for them.
Just what exactly avenues would crictally ill and dying patients need to put themselves from misery? Apart from with regards to the medical profession to ensure they are comfortable or taking drugs that can relieve their suffering, there is not much you can do. Many people when we were in that situation will be too weak to tug ourselves out in to the snow.
Altruism is thus within many professions, just one cannot deny the fact doctors, nurses and non secular missionaries has to be viewed as front-runners. This is because individuals who enter these professions are genuinely on the market to accomplish the right in society possibly at the same time feel great about themselves. Hence look for the maximum assortment of helpers and ego-trippers gathered on a sunny day from the medical profession. Undoubtedly doctors are generally nice obliging fellows, always willing to help their patients. These chaps find it difficult to avoid their clients since they subconsciously feel bad whenever they said no.
However, all is not lost. As I find it, those involved with this terminal situation who have any clues about their mind and have retained some chance to think, can still utilize the power of their depths of the mind to exit our planet peacefully. All they should do is always to understand the fact that death is a only a beautiful sleep from where one never awakens. It becomes an eternal sleep.
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Description du Produit
Functions : 15 Gbps 400 mVpp->8Vpp Output single Driver SMA Housing
The VWA-15-VA-257 is a broadband dual stage GaAs MMIC amplifier module which provides up to 25 dB gain from 55 kHz to 15 GHz and delivers +21 dBm of output power with a single Voltage regulated Bias. The output voltage can be set between 4 to 8 Vpp in varying the Pin Vctl.
- Very Low Jitter
- Very Low rise and fall time (bellow 17ps)
- Output Vpp control
- Ultra Wideband
- Very High Gain
- Medium power
- 50 Ohm impedance
- Single +12V supply
Main applications include: Long Haul Medium Haul Metro Access Test Bench Test & Measurement.
This unit performs LN and EAM driver functions for 10.7 Gb/s RZ data rates and 2.5 Gb/s 5 Gb/s 9 958 Gb/s and from 10 7 Gb/s to 12.5 Gb/s for NRZ data rates. The driver amplifier has singleended input and output and SMA field replaceable RF connectors (male or female)