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Read the latest issue of the ProbesOnline E-Newsletter.
In This Issue
|No-Wash Apoptosis Imaging — CellEvent™ Caspase-3/7 Green Detection Reagent
|Sensitive Oxidative Stress Detection — CellROX™ Deep Red Reagent
|New Antibodies for Secretory Pathway Research — Recombinant Syntaxin (Pep12) Antibodies
New! BioProbes 64 is Now Available Online
what it is
The CellEvent™ Caspase-3/7 Green Detection Reagent is a new reagent for imaging apoptosis that does not require any wash steps, is compatible with high-content imaging, and is well suited for multiplex imaging in live or fixed cells.
what it offers
- Simple, no-wash protocol preserves delicate apoptotic cells
- Suitable for live-cell imaging, enabling continuous monitoring of dynamic events
- Compatible with formaldehyde-based fixation methods
how it works
The CellEvent™ Caspase-3/7 Green Detection Reagent consists of the DEVD peptide sequence conjugated to a nucleic acid–binding dye. The substrate is intrinsically nonfluorescent, as the DEVD peptide inhibits the ability of the dye to bind to DNA. In the presence of activated caspase-3/7, the DEVD peptide is cleaved, enabling the dye to bind to DNA and produce a bright fluorescence response. The fluorescence emission of the dye when bound to DNA is ~530 nm and can be observed using a standard FITC filter set.
- Learn More About the CellEvent™ Caspase-3/7 Green Detection Reagent
Multiplex measurements of apoptosis and oxidative stress.
HepG2 cells were treated with 50 µM nefazodone or DMSO (control) for 24 hr. During the last 30 min of treatment, the cells were stained with 5 µM CellEvent™ Caspase-3/7 Green Detection Reagent, 5 µM CellROX™ Deep Red Reagent, and Hoechst 33342. Treated cells ( B) showed clear increases in both oxidative stress (red) and apoptosis (green), compared to control cells ( A).
|Cat. No.
|CellEvent™ Caspase-3/7 Green Detection Reagent, 2 mM solution in DMSO
|100 µL
CellROX™ Deep Red Reagent is a novel fluorogenic probe for detecting oxidative stress in cells. The bright, deep red–fluorescent signal is compatible with other live-cell dyes and GFP, making it useful in multiplex fluorescence assays to measure a variety of cellular phenomena, including cytotoxicity and cell death. Unlike many other ROS sensors, the signal from CellROX™ Deep Red Reagent is retained after formaldehyde fixation.
what it offers
- Compatible with GFP and Alexa Fluor® 488 dye
- Suitable for live-cell imaging or formaldehyde-based fixation
- Validated with multiple platforms
The cell-permeant CellROX™ dye is nonfluorescent in a reduced state and exhibits bright fluorescence upon oxidation by reactive oxygen species. Its emission maximum of ~665 nm is measurable by fluorescence imaging, high-content imaging, fluorescence plate readers, or flow cytometry.
- Learn More About CellROX™ Deep Red Reagent
Detection of oxidative stress using CellROX™ Deep Red Reagent.
BPAE cells were plated in a 96-well plate, treated with 100 μM menadione for 1 hr to induce oxidative stress, then stained with 5 μM CellROX ™ Deep Red Reagent, 20 nM MitoTracker® Green, and Hoechst 33342 for 30 min in complete medium. Cells were then washed 3 times with PBS. The control cells (left) show no signal, while the menadione-treated cells (right) show a robust increase in signal as a result of oxidative stress.
|Cat. No.
|CellROX™ Deep Red Reagent, for oxidative stress detection
|5 x 50 µL
what they are
Yeast syntaxin (Pep12) is a key component of the endocytic pathway. Localized in the prevacuolar compartment, syntaxin plays a role in trafficking endocytic cellular contents between early or recycling endosomes to the vacuole. Dysfunction in vesicle trafficking has been recently implicated in metabolic diseases. We now offer highly specific ABfinity™ recombinant antibodies against syntaxin.
what they offer
- High sensitivity and specificity
- Excellent lot-to-lot consistency
- Extensive validation and characterization
how they work
ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones. This technology helps to ensure consistent antibody performance, so you don't have to reoptimize your assay with each new lot.
- Learn More About ABfinity™ Recombinant Rabbit Monoclonal Antibodies
Western blot detection of yeast syntaxin.
Syntaxin was detected with Syntaxin PEP12 Abfinity™ Recombinant Rabbit Monoclonal Antibody (left) or Syntaxin PEP12 Abfinity™ Recombinant Rabbit Oligoclonal Antibody (right). Antibodies were used at 1 µg/mL with 1 ng/lane Rec Yeast Syntaxin+Fusion Protein. The western detection was performed using a WesternBreeze® kit with NBT/BCIP as the substrate.
|Cat. No.
|Syntaxin PEP12 ABfinity™ Recombinant Rabbit Monoclonal Antibody
|100 µg
|Syntaxin PEP12 Recombinant Rabbit Oligoclonal Antibody
|100 µg
A full understanding of autophagy requires the measurement of changes in other cellular events that occur in parallel. The LC3B Antibody Kit for Autophagy, when used with Click-iT® kits, allows researchers to study a myriad of cellular events—including cell proliferation, RNA transcription, protein synthesis, protein lifetime, apoptosis, and posttranslational modifications of proteins—in conjunction with autophagy.
The LC3B protein normally resides in the cytosol. During autophagy, LC3B is cleaved and lipidated with phosphatidylethanolamine, and associates with the autophagosome as it forms. This association with the autophagosome makes it one of the most reliable markers for autophagy. Click-iT® kits can be used to measure cell proliferation (Click-iT® EdU), nascent protein synthesis (Click-iT® AHA), apoptosis (Click-iT® TUNEL), changes in newly synthesized RNA (Click-iT® EU), and posttranslationally modified proteins (via the incorporation of various "clickable" sugar analogs). Click-iT® detection occurs through the copper-catalyzed covalent reaction between an azide and an alkyne, one of which bears a fluorescent reporter such as an Alexa Fluor® dye. Click-iT® kits are available in a variety of colors and are multiplexable with the LC3B Antibody Kit as well as many other assays, markers, and antibodies.
|Multiplex analysis of LC3B accumulation and other parameters of cell function.
The induction and inhibition of autophagy can be combined with other measures of cell health. Click labeling was combined with LC3B staining in HeLa cells after treatment with chloroquine. Click-iT® detection was used to label (A) proliferating cells (Click-iT® EdU, pink; rabbit anti-LC3B, green), (B) protein lifetime (Click-iT® AHA, green; rabbit anti-LC3B, red), (C) RNA transcription (Click-iT® RNA, pink; rabbit anti-LC3B, purple), or (D) posttranslational modification with N-acetylated galactosylamine (Click-iT® GalNAz, green; rabbit anti-LC3B, blue). EdU and EU were fed to cells after drug treatment, whereas GalNAz and AHA were fed to cells before drug treatment. All Click-iT® reactions were carried out using Alexa Fluor® 488 dye conjugated to either an alkyne or azide. Cells were costained with Hoechst 33342, anti-LC3B rabbit IgG, and Alexa Fluor® 647 dye–conjugated goat anti-rabbit secondary antibodies.
|Cat. No.
|LC3B Antibody Kit for Autophagy
|1 kit
|Click-iT® EdU Alexa Fluor® 488 Imaging Kit
|1 kit
|Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay, 2-plate size
|1 kit
|Click-iT® RNA Alexa Fluor® 488 HCS Assay, 2-plate size
|1 kit
|Click-iT® EdU Alexa Fluor® 488 HCS Assay, 2-plate size
|1 kit
|Click-iT® GalNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-azidoacetylgalactosamine), for O-linked glycoproteins
|5.2 mg
|Zenon® IgG Labeling Kits are the fastest way to noncovalently label very small amounts of rabbit and mouse IgG antibodies with fluorophores, biotin, or fluorescent proteins (e.g., R-PE). Zenon® labeling technology uses a labeled Fab fragment directed against the Fc portion of an intact IgG antibody to form a labeling complex. Because the Zenon® labeling method is based on immunoselectivity, there is no need to remove exogenous proteins or amine-containing buffers from the antibody sample before forming the complex.
The entire labeling procedure takes only 10 minutes, and nearly 100% of the primary antibody is labeled. Zenon® labeling technology dramatically simplifies time-consuming immunocytochemical applications such as the use of multiple same-species–derived primary antibodies in one staining experiment. The extensive selection of available labels and the versatility of Zenon® technology make it easy to experiment with different color combinations for optimal multicolor images. Quick and convenient, Zenon® labeling requires only 1 µg of primary antibody for the entire labeling procedure. The resulting Zenon® complex is also fully compatible with most flow cytometry applications.
Use of Zenon® labeling technology in fluorescence imaging. Fixed and permeabilized bovine pulmonary artery endothelial cells were stained with Alexa Fluor® 594 phalloidin and Hoechst 33342 to label actin filaments and the nucleus, respectively. Anti–OxPhos Complex V inhibitor protein antibody was complexed with the Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit to stain the mitochondria.
|Fluorescence SpectraViewer — There's an App for That
Plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab. This mobile SpectraViewer acts as an extension of our online Fluorescence SpectraViewer:
|Imaging the Golgi complex, mitochondria, and cytoskeleton in fibroblast cells. Albino Swiss mouse embryo fibroblast cells (3T3) were stained with an Alexa Fluor® 750–conjugated anti-giantin antibody (Golgi, yellow; prepared using Alexa Fluor® 750 succinimidyl ester), MitoTracker® Deep Red stain (mitochondria, blue), Alexa Fluor® 488 Phalloidin (actin, green), an Alexa Fluor® 569–conjugated anti-vimentin antibody (intermediate filaments, red), and Hoechst 33258 (nucleus, cyan). Image contributed my Michael W. Davidson, Florida State University.
|Cat. No.
|Alexa Fluor® 488 Phalloidin
|300 units
|Hoechst 33258, 10 mg/mL solution in water
|10 mL
|MitoTracker® Deep Red FM, special packaging
|20 x 50 µg
|Alexa Fluor® 750, succinimidyl ester
|1 mg
Amplex® UltraRed Enhances the Sensitivity of Fluorimetric Pyruvate Detection
Zhu A, Romero R, Petty HR (2010) Anal Biochem 403:123–125.
Indicators of intracellular pyruvate levels are important tools for investigating cell growth, signaling, and metabolism. One assay commonly used to measure pyruvate includes horseradish peroxidase (HRP) and pyruvate oxidase, which have better activity in acidic environments than in basic or neutral solutions. Zhu and colleagues recently reported the results of pyruvate measurements obtained using the fluorescent indicator Amplex® UltraRed Reagent. Because the Amplex® UltraRed reaction product has a lower pKa than resorufin (the product of the previous indicator, Amplex® Red), the researchers were able to collect data using lower-pH buffers, which are more suited to the enzymes in their assays. The authors also noted that the Amplex® UltraRed reaction product exhibited higher fluorescence over the pH 5–7 range compared to resorufin, and higher Z′-factor values (0.93 for Amplex® UltraRed compared to 0.89 for Amplex® Red).
- View the Bibliography Reference
- Learn More About Amplex® Red Enzyme Assays
|Cat. No.
|Amplex® UltraRed Reagent
|5 x 1 mg
The Molecular Probes® Handbook
© 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Feel free to distribute ProbesOnline to friends and colleagues, but please keep this copyright statement intact.


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NormanD wrote:Was your weekend that bad??
No, but the working week is looking decidedly iffy.
It is currently Mon Sep 16, 2019 5:30 am
NormanD wrote:Was your weekend that bad??
NormanD wrote:Nah - they turned it into 'a dream' in this version.
Jamie Renton wrote:Burning Spear - Jah No Dead (from the film Rockers) https://www.youtube.com/watch?v=Avt7wpBDn6g
Adam Blake wrote:Tom Waits: Get Behind The Mule
One of his best, I think. Charlie Musselwhite on harmonica.
Jamie Renton wrote:UK based fadista Claudia Aurora - Poema.
From her new album Mulher Fo Norte which I'm liking a lot
NormanD wrote:Does she play high-profile gigs in the UK, or would Jamboree be below her league?
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text:
Toro Power Clear® 518 ZE (38473)
Power Clear® 518 ZE (38473)
The Power Clear 518 ZR features a Toro Premium 99cc OHV 4-cycle engine with electric start. Power Curve® Rotor system pulls you forward clearing compacted snow right down to the pavement and throws snow up to 25' (8 m) away. 18" (46 cm) inch clearing width and 12" (31 cm) intake height. Ideal for concrete and asphalt surfaces. Recommended for 2 - 9" (5 - 23 cm) of snow and up to 4+ car driveways. Fold-down handle and chute make storage or transport simple. Storage dimensions: 29.5" x 18.7" x 27.5" (75 cm x 48 cm x 70 cm). All Toro single stage snowthrowers are backed by Toro's 2-Year Full Warranty and Two-Year Guaranteed to Start Warranty (Residential only).
- Powered by a Toro Premium 99cc OHV 4-cycle with Electric Start, it's ideal for concrete and asphalt surfaces that can park up to 4+ cars and 2 - 9" (5 - 23 cm) of snow at a time
- Power Curve Technology - curved rotor and inverted funnel housing efficiently remove snow while reducing clogging
- 18" (46 cm) Clearing Width, 12" (31 cm) intake height, Up to 25' (8 m) Throw Distance
- Put the snow exactly where you want it with the Locking Zip Deflector
- #1 Brand of U.S. Single-Stage Snowblowers: Our best-selling snowblowers are strong enough to take on serious snow, yet easy for anyone to maneuver.
- Toro Premium 4-cycle OHV Engine: The smooth Toro Premium 4-cycle OHV engines provide years of dependable and powerful performance. These engines have been tuned specifically for the machines they power to provide optimal performance. With unmatched governor response, these engines deliver power faster when you need it most.
- Power Curve® Technology: This technology, with curved rotor and inverted funnel housing moves more snow in less time and virtually eliminates clogging. Wide rubber paddles propel the snowthrower forward while cleaning down to the pavement.
- Locking Zip Deflector Power Clear: Throw snow high, low or in-between. The deflector locks in to any angle in less than a second-with just a touch of your hand. (Select models only.)
- 2-Year Full Warranty: This product is covered by a two-year full warranty. See your dealer for warranty details.
- 2-Year Guaranteed-to-Start Warranty: This product is covered by a two-year Guaranteed-to-Start warranty. See your dealer for warranty details.
- Auger System: Power Curve® Rotor
- Chute Control: Chute-Mounted Handle, Curved w/Zip Deflector
- Clearing Width: 18" (46 cm) clearing width, 12" (31 cm) intake height
- Engine: Toro Premium 99cc OHV 4-cycle
- Fuel Capacity: 1.6 Quarts (1.5 L)
- Scraper: Stationary
- Starter: Electric Start
- Throw Distance: Up to 25' (8 m)
- Tires: 6" x 1.5" (15 cm x 3.8 cm )
- Warranty: 2-Year Full / 2-Year GTS (Residential Only)
- Weight with Electric Start: 58 lbs (27 kg)
NOTES:*Throw distance will vary with conditions.
- Engine Type
- Premium OHV 4-cycle
- Engine Manufacturer
- Toro®
- Width
- 18 in. (46 cm)
- Starting
- Electric Start
- Max Discharge
- Up to 25 ft. (8 m)
- Wheel Size
- 6 in. x 1.5 in. (15 cm x 3.8 cm )
- Weight
- 58 lb. (27 kg)
- Fuel Capacity
- 1.6 qt. (1.5 L)


text:
How to choose sustainable flowers
June 22, 2018, 2:33 p.m. by Siel Ju
What's the best choice: Organic, local, fair trade or silk?
Buy a snack, give a meal
August 17, 2011, 6:49 a.m. by Siel Ju
For every Two Degrees snack bar you buy, the company will send a nutrition pack to a child suffering from malnutrition.
A festival for good, sustainable food
August 16, 2011, 6:56 a.m. by Siel Ju
A 5-day Good Food Festival and Conference will bring together farmers, sustainable food advocates, chefs, and foodies to Santa Monica., Calif.
Organic tea for lemongrass lovers
August 15, 2011, 1:40 p.m. by Siel Ju
Enjoy the sweet flavor of lemongrass -- in an organic, fair trade certified tea.
Eco-friendly customized cards
August 12, 2011, 2:55 p.m. by Siel Ju
Need personalized stationery or paper event invites? Paper Culture offers customized cards printed on 100-percent recycled, FSC-certified paper.
Green Facebook games
August 11, 2011, 3:09 p.m. by Siel Ju
Two new Facebook games aim to teach players about conserving wildlife and upcycling trash — while tackling real-life environmental issues.
Grab-and-go superfood snacks
August 10, 2011, 4:33 p.m. by Siel Ju
Superfood lovers can snack on the go with a new line of ready-to-eat treats made with nutrition-packed raw ingredients like chia and maca.
4 organic rooibos teas
August 9, 2011, 2:55 p.m. by Siel Ju
MNN's lifestyle blogger put four organic rooibos teas to the taste test.
Kayak through Los Angeles
August 8, 2011, 11:04 p.m. by Siel Ju
Visiting the Golden State this summer? Take advantage of a rare opportunity to kayak and canoe down the Los Angeles River while getting educated on urban ecology.
See you at BlogHer '11
August 4, 2011, 5:45 p.m. by Siel Ju
Meet MNN's lifestyle blogger at the BlogHer '11 conference in San Diego this weekend.
Chia pudding: A breakfast rich in omega-3
August 4, 2011, 6:14 a.m. by Siel Ju
Switch from hot oatmeal to cool chia pudding for a chilled, healthy breakfast on a sweltering summer day.
Beauty from organic argan oil
August 3, 2011, 12:45 p.m. by Siel Ju
An all-organic, chemical free beauty line from La Bella Figura impressed MNN's lifestyle blogger with its argan oil-based eco-luxe products.
Your 2019 fall foliage guide
Now you know the best time to be a 'leaf peeper' in any part of the U.S.
Why do leaves change color in autumn?
Learn about the natural mechanisms that produce breathtaking fall foliage each year.
Test your fall knowledge
The days are getting shorter and pumpkin spice seems to be everywhere: It must be fall.
10 best places outside the U.S. for fall color
These places around the world that equal U.S. locales when it comes to colorful scenery.
5 classic fall recipes
Roasted veggies and soups of all kinds are what we're talking about.


text:
Bobrovsky, Blue Jackets blank Philadelphia 2-0
PHILADELPHIA (AP) — Sergei Bobrovsky stopped 37 shots for his fourth shutout of the season, leading the Columbus Blue Jackets to their first win in Philadelphia, 2-0 over the Flyers on Thursday night.
James Wisniewski and Brandon Dubinsky each scored goals for the Blue Jackets, who were 0-5-1 in Philadelphia since the franchise's inception in 2000.
Led by Bobrovsky, a former Flyer, the Blue Jackets moved closer to clinching their second-ever playoff berth. They closed within two points of the Flyers for third place in the Metropolitan Divison.
The Flyers have lost three straight games and were shut out for the second straight time.
Wisniewski snapped a scoreless tie with his seventh goal of the season late in the second period. He finished off a Columbus attack that saw at least three shots on Steve Mason before he was able to slide in the puck with 6 seconds left on the power play.
Dubinsky appeared to kick in the puck early in the third to make it 2-0.
The Blue Jackets are about as automatic as it gets in the NHL with a lead after two periods: They improved to 27-2-4 with a two-period lead.
The interesting matchup was the one between the pipes: Mason vs. Bobrovsky.
Mason had it all figured out in Columbus when he went 33-20-7 with 10 shutouts and won the Calder Trophy as rookie of the year in 2008-09, the only season the Blue Jackets have made the playoffs.
But Mason and Columbus could never recapture that fleeting success again. He fell to 20 wins and five shutouts the next season, and bottomed out in 2011-12 with a 16-26-3 mark. Mason lost his job to Bobrovsky, the former Flyer who blossomed into last season's Vezina Trophy winner.
Mason was traded to the Flyers at the end of last season and became the No. 1 goalie after they bought out Ilya Bryzgalov's contract.
Bobrovsky was expendable because Bryzgalov signed a nine-year deal and was supposed to anchor the net as Philadelphia chased its first Stanley Cup championship since 1975. Bryzgalov's quirky personality and sometimes brutal honesty with the media didn't always endear him to teammates and he lasted only two seasons.
Mason and Bobrovsky are now leading playoff pushes for both organizations.
NOTES: Flyers D Kimmo Timonen (upper body) returned to the lineup after a one-game absence. … Bobrovsky has eight career shutouts. … The Blue Jackets are 3-1 vs. the Flyers this season. … The Blue Jackets and Flyers have played each other only 15 times.


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These are the first images from the top of Broadway Malyan's under-construction One St George Wharf tower, in Vauxhall, London
Sat south of the Thames next to the much-maligned St George Wharf housing project, which was also completed by the practice for developer St George, the 180m-tall skyscraper is set to become London's tallest residential-only building.
Each typical floor of the 50-storey building will be divided into five apartments with separating walls radiating out from the central core so that every flat has views across the capital.
Dubbed 'the battery', the cylindrical tower started on site in March 2010 and is scheduled for completion in June 2013.
The architect's view
Peter Crossley, Director at global architecture, urbanism and design practice Broadway Malyan, said: 'The Tower is an elegant and timelessly classic piece of design. It is exceptionally sustainable and will act as a catalyst to regeneration in the surrounding neighbourhood.
We expect that it will quickly become a familiar and friendly landmark to Londoners and visitors alike, following in the footsteps of other buildings of similar scale and mass.
Our detailed studies have shown that this is the right building for this location and, due to its small footprint and visually subtle circular plan form, The Tower will have exceptionally slender pencil-like proportions when viewed from any direction.'
Broadway Malyan to rethink disputed Vauxhall skyscraper
KPF to redevelop 1970s towers in Vauxhall
Allies and Morrison submits Vauxhall twin towers
Exclusive images from Europe's tallest residential tower
Readers' comments (5)
It is not elegant. It is not friendly. It has no sublety. Wherever you are over a wide area it grimaces at you over the roof tops or in between buildings at street level. It is over assertive. Has no relation to its surroundings. The regeneration of this area was already under way without this very unfortunate addition to the Thames riverscape and London's skyline. Thjis practice has done and can do far better than this.
Owen Luder .
the fact this project is meant to in some ways compete with one hyde park is laughable, thank god the view is good to keep your attention away from the bland interior and sub standard finishes.
sorry apart from the new covent garden view which is circa eastern block rustic
How disappointing. Oh what could have been, the context, height, visibility – a landmark, a vision of the future… the possibilities. Instead a snapshot of an American past bookending what looks like a Kuala Lumpur Hospital. It is clear that size isn't everything.
Owen is our leader.
If nothing else, it distracts the eye from the embarrassing architecture of the adjacent 'Emerald City'.
Less favourably, it also detracts from almost every meaningful view in Westminster: crassly backstaging UNESCO world heritage sites; ominously looming at the end of every major axis in Pimlico; and dwarfing the accumulator tower at Churchill Gardens, which so far as I can tell, is its only architectural point of reference locally.
By the time the rest of Vauxhall and Nine Elms is built out, planners will be turning their attention to dealing with the blight of St George Wharf. The term 'regeneration', when applied to this affair, is little more than a sad irony.
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text:
That Crowning Touch
Ok, bakers, time to make some princess tiaras!
Now remember, we want them to look like this:
Because reasons.
And we can only make cupcake cakes (patooie!), because cupcake cakes (patooie!) are just so clean and beautiful and easy to serve:
[deadpan stare]
Aaaaand... GO.
[wincing] Oooh, SO CLOSE.
Less close.
(Am I the only one who sees a star stepping over a puddle?)
What the...?
[softly banging head on desk]
I give up.
Let's just go back to making King cakes.
Thanks to Liz, Chris L., Ann Marie P., Kristin M., Hannah C., Valerie B., & Erich B., who totally rule.
Thank you for using our Amazon links to shop! USA, UK, Canada.


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text:
We have arrived. 37 weeks is full term and we reached that milestone on Sunday- Yipee!
Today Carey and her step mother, Marie (who has been such an amazing support through all of this) went for the cerclage to be removed. It was an in-office procedure, that went better than she had hoped for. If there were any complications removing the stitch, Carey would have gone in tomorrow for a spinal block and removal in the "O.R." (needless to say we didn't want that to happen). The stitch was removed in about 3 mins. There was some blood on the doctor's hands and Carey continues to bleed a little, which the doctor said was normal. This next part is only documented for readers curious about cerclage removal...it may be Too Much Information (TMI)for those not otherwise interested... Carey said there was a clot that came out after all was said and done. I'm guessing maybe it could be the equivalent of a mucous plug? We never did get a firm answer as to whether or not she still actually had a mucous plug after delivery of Owen- apparently no one knows... Anyhow- now that you are thoroughly grossed out...
The doc said Carey is 1cm dilated and 80% effaced. Google searches are no help as to this being indicative of impending labor within a certain time frame. So I guess this leaves us like everyone else- playing the waiting game until "D-day".
Yes, the bags are FINALLY packed! I've asked Carey to work on a birthing plan today, but my guess is that the 2 percocet that they gave her for pain will take her out for the afternoon and I'll breathe easier some other time.
We have worked on my list of TO DO's all weekend, however, and made some major progress. We cleaned the entire house, packed the baby's bag and both of our bags, washed the 2 vehicles, installed the carseat, listed items on ebay, went to a birthday party, saw a movie, fixed the kid's bikes,bathed and groomed the dog(at home-woof!), went grocery shopping, did all of the laundry, packed the kids for summer camp on Sunday, and a bunch of other miscellaneous tasks. All that to say- we were productive this weekend- and worked together to get it done.
When I rewrote my list last night (minus our accomplishments) it was still pretty lengthy- but much more manageable now. Thank you Carey :) I need a freakin' Xanax. :)
Sorry, no pics of the cerclage removal are available hahaha... Instead, I'll leave y'all with a video I enjoy watching for a laugh every now and then- if you don't know the song, it won't be as funny- but even if you don't know the song you have to laugh. Til next time...
Tuesday, June 1, 2010
Cerclage Removal Complete!
We have arrived. 37 weeks is full term and we reached that milestone on Sunday- Yipee!
Posted by Janine at 1:04 PM
Labels: cerclage removal
4 comments:
- EmmaJilly said...
aww, i'm glad things are good so far. hope everything goes well and the little one is with you soon x
- June 1, 2010 at 2:41 PM
- Michele said...
So glad to here all is well! Can't wait to hear that he's arrived!!!
- June 1, 2010 at 3:29 PM
- Kate said...
So excited for you- bummed about the lack of pictures. :) I had my twins at 37 weeks- I was done- so I talked the Dr. into a c-section.
Won't be long now- so excited for you guys to meet the baby- great job keeping the little one in for 37 weeks!
- June 1, 2010 at 10:00 PM
- COUNTRY MOM said...
Checking in to see how things are going. How are you feeling? Glad you made it full term. Hope to hear a great birth update soon. Blessings,
- June 8, 2010 at 1:03 AM


text: This compact 5" x 7.375" (12.7 cm x 18.7 cm) Super Pencil Slate will fit into most BCD pockets, yet has plenty of room for taking notes, conversing with your dive buddy, or writing down skill performance when training. Whatever your writing needs are this slate can get it done. Super Pencil Slate comes with a super pencil, unlike more conventional dive slates the super pencil has replaceable lead, so once you use it up you can get a refill for years of continues use. Replaceable lead for Super Pencil, (see SKU: AQUSPSRP). Slate is also equipped with a corrosion resistant locking gate clip and nylon webbing loop for easy secure to your equipment and protective pencil tube for storage of the super pencil.


text:
Baby and Pet Safety Tips
Introduce them slowly
Your pet sees your home as his turf, so don't expect him to immediately accept baby into it. "It can take two to three months for a dog to accept a new animal into a home, so you should assume the same for an infant," says Sam Basso, a professional dog trainer and behaviorist in the Phoenix area.
Ease the transition by introducing them slowly—this can help head off territorial behavior. "Greetings are extremely important for a dog to distinguish welcome visitors from unwelcome intruders," says Basso. Start by bringing the dog to a neutral place, far from his territory—like a local park—then let him sniff baby's blanket and introduce him to baby. Once your dog seems comfortable, you can move to step two, which is running through that same scenario over again right outside your house, before you bring baby into the home.
Give your pet a hideaway
A stressed dog could act out, so give him his own baby-free zone. "Having his own spot where children are instructed to leave him alone will encourage your pet to go there when he has had enough play and needs a break," says Lynn Buzhardt, DVM, veterinarian and coauthor of Can We Have One?: A Parent's Guide to Raising Kids With Cats and Dogs. It doesn't have to be anything elaborate—a cozy cat bed too high for little hands to reach or a favorite corner of a room with a soft blanket.
Model the right behavior
Pets can get a lot of (usually unintentional) abuse from small children. Teach your child the right way to treat animals in more ways than just saying "no" when he's yanking your pet's tail and ears. "Teach children how to interact with the animal by modeling it for them and helping them practice," Buzhardt says. If your child hits the cat, take her hand and show her how to gently stroke its fur. Explain why they need to touch animals gently; even if your dog is a gentle giant, learning this can help a child be safer when encountering dogs on the street or friends' pets on playdates.
Give your pet plenty of exercise
Pretty much everything will revolve around baby, but don't forget to give Fido the exercise and care he needs. "If a dog is neglected, it can get jealous of baby, or become overly focused on baby," says Basso. If you're too exhausted to keep up with your usual walk or play schedule with your pet right now, cut yourself some slack—see if you can get a friend to stop by and take your dog out for a stroll, or a neighborhood kid to play with your cat and some toys. "If you need a place to put the dog on those days when you are exhausted, a local doggie day care is ideal," Basso suggests. "The dog is getting exercised and socialized, and is getting more general stimulation—which means the dog will be calmer and easier to deal with."
Never leave them alone together
It doesn't matter if your dog has the sweetest disposition or your kitty won't hurt a fly—never, ever leave your baby or toddler and pet alone in a room together. "If the parent leaves the room, either the animal or the baby goes with," Buzhardt says. (We know it's hard, but it's important!) It will be years before your child will be able to handle the pet on his own.
Plus, more from The Bump:
Watch: Can Your Pet Predict Pregnancy?


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|System: X360, PS3
|Review Rating Legend
|Dev: EA Canada
|1.0 - 1.9 = Avoid
|4.0 - 4.4 = Great
|Pub: EA Sports
|2.0 - 2.4 = Poor
|4.5 - 4.9 = Must Buy
|Release: Nov. 17, 2009
|2.5 - 2.9 = Average
|5.0 = The Best
|Players: 1-4 (10 Online)
|3.0 - 3.4 = Fair
|ESRB Rating: Everyone
|3.5 - 3.9 = Good
by Leon Hendrix III
I reviewed NCAA Basketball 2009 last year around this time, and I have to say right off the bat not much has changed. Actually, that's not entirely accurate. I should clarify. The game is a whole heap of a lot better in certain, very distinct ways. I wasn't the first to point it out, but I'd like to jump on the bandwagon and say that most of those ways are very similar to the recently released NBA Live 10. Still, as a fan of college sports, I have to say that NCAA Basketball 2010 is a strong entry in the series even if it's reminiscent of years past. Presentation is better than ever, play-calling has improved, and EA's collegiate level focus on tempo and crowd involvement is stronger than ever. I just hope you haven't played Live or NCAA 09 in a while.´
I hate to always be Captain Buzzkill, so let's start with the good things, and there are plenty of good things about NCAA Basketball 2010. One of the first things you'll probably notice about the game is the all-new (at least to NCAA) Motion Control offense. Essentially, this is a hotkey that maps offensive play-calling to the left shoulder button. As I remarked about the feature in Live; while not entirely revolutionary, it definitely streamlines gameplay a bit without removing the strategy that makes the game more than a shoot-out. As a side-note, I've considered running plays and strategy obsolete and unnecessary in the all-star swish-fest known as 'Live' for a few years now. Yet in NCAA 2010, it makes a strong comeback. While it is entirely possible to run and gun or pick and roll all game, the best defense really is a good offense - or a well run one.
With a tap of the button, the ball handler will call the motion and send players screening and cutting across the court. When held, the shoulder button brings up a quartet of signature plays, each mapped to one of the face buttons. While these plays don't feel overly powerful, they can be quite effective when played to their strengths (feeding a backdoor cut, luring the double team and kicking to the perimeter for the three, etc.). The system isn't perfect, but it can slow gameplay down considerably and, when used properly, can offer a real advantage. For once in a sports game, it's actually gratifying to see a play called and run and not just watch star players knock down improbable shots.
The gameplay scales nicely and most games won't be blowouts. Offense is all about finding and taking the intelligent shot, controlling the floor, and keeping the ball out of defenders' hands, while defense boils down to controlling the paint, limiting second chance opportunities, and playing the passing lanes (this is very effective). This pace can be realistic to a fault. I played a rivalry game as Wake Forest against NC State and won by ten or twelve. During the last two minutes of the second half NC State fouled to stop the clock, naturally. At first it seems like a smart touch, except that it's nearly impossible to avoid the defenders. It may be true to life, but it's very annoying. Gamers hoping to smash their opponents by fifty won't necessarily be unable to, but it is important to note that fast break points, outside shots, and the other staples of that kind of offense are tough to come by. It's up for debate, but I think it makes for a much better game.
Fans of the "home court advantage" will be thrilled to find that EA pushes the envelope yet again with the Toughest Places to Play. Apparently, fans around the country voted (although when I'm not so sure) and EA selected the top 20 arenas where the crowd is a factor. It's an interesting idea and it plays a significant, though perhaps not game-changing, role. When you enter one of these arenas, chants shake the camera during key moments, free throws are tougher (though I'm not a fan of that mechanic anyway), and you really get the sense that you're playing under the scowling eyes of thirty thousand fans. What's more interesting is the fact that a team can earn its way on the list by winning during dynasty mode. Kind of a nice gesture to all of the fans of college hoops whose teams don't play in North Carolina.


text: I knew Nick since I was in seventh grade. He was in my sister's grade, 4 years older. I always had a crush on him. I thought he was so funny and he seemed like a genuinely nice person. Of course I was much too young, so I kept these feelings to myself. In 9th grade, he was in 12th grade. I remember my sister left me at the school, without a ride. He immediately offered me one and I blogged about it, embarrassingly enough, in my livejournal. Finally, freshman year of college, Nick went with my family to my sister's graduation ceremony. He was the type of loyal guy that would do anything for anybody. Even without a job at the time, he bought new shoes and new clothes for the occasion. All day we went laughing together and just having an amazing time. We hung out all night, and every day after. With Nick everything was so simple. I liked him, and he liked me. We went together so well. After three years of dating, we decided to move in together. The best moment of my life was coming home and seeing him unpacking all of his things in the living room with the record player playing. I bought a bottle of champagne, and we kept the bottle on the shelf to remember that moment. Living together was so easy. We got along so well, could tell each other anything. We were comfortable staying home and watching Netflix, drinking wine. We were also comfortable going out with friends, or going out alone. Nick wasn't the jealous or untrusting type. With Nick, I was a motivated, happy person. Everything in life was figured out. As long as I had Nick in my life, I didn't care too much where I was going. I had that one person that would go through life with me. Sometimes when I would get overly stressed or worried, he was always there to relax me and tell me how amazing and wonderful I was. A few months before we moved in together, Nick was getting sick. We weren't sure exactly what it was but it could have been Crohns disease. Nick saw specialists and doctors, but he lost he insurance for a few months at the end of the year. He was scared for the debt and the uselessness the doctors could be at times so he didn't go. He got weaker and weaker. Eventually, he had to take a leave of absence from work until his insurance came back in a few months. During this time, he got so weak and pale. It scared me. Many times I asked him "Are you going to be OK?" "Are you going to make it thru?" "Do you want me to take you to the emergency room?" and he always insisted he was okay and he was going to make it. I guess I never knew the full extent of what could happen at that point. I was worried, but I was optimistic that he was going to get better. Finally, in January, he got he insurance back and went to the specialist. They told him he didn't need to be hospitalized, that he just needed to wait on some bloodwork. A few days later, he and his mom decided to go to the emergency room. They had enough of waiting and Nick was getting drastically worse. When he got there, he had a seizure. I remember waking up from my nap and getting a call from his mom giving me the news. She reassured me that he was in good care and was going to be okay. I drove to the hospital, only to get worse news. They sat me down in a room and told me that he wasn't breathing on his own, and his heart stopped pumping and they were performing CPR. This was the worst feeling in the world. I was in shock and confused. Finally, they got him stabilized and we were able to see him. It was scary seeing him there on the hospital bed. He wasn't conscious, but he still talked to him, encouraging him to make it I couldn't help but laugh because he was naked except for pink fuzzy socks. This was typical Nick. He just wanted to be comfortable. They were able to transport him to the other hospital, where the care was more sufficient. When we arrived at the other hospital, they told us that his heart wasn't beating on it's own. It was another hour and a half until they came out again. The doctor sat us in a room and said things weren't looking good. His heart was beating again, but he was the sickest patient in Grand Rapids and he had a laundry list of problems. I remember looking out the window, so confused and wondering what I would do without him. The doctor assured us that they had a great team of specialists working on him and they were trying their hardest. It was just time to wait. We waited in the hospital for ten hours, hearing news of his body improving. I was so optimistic. Nick was the type of person that a bad thing would happen to him, but somehow he ended up unscathed. I told his best friend this and said that I would hit Nick on the back of the head for being so stubborn about getting care sooner, laughing. Finally, after midnight, they did the catscan. They warned us that since his brain went without oxygen for some time that there was a chance that he would have no brain activity. When the doctor brought us in the room, I wanted the news right away. He made sure everyone was in the room and this scared me. Finally, he informed us that Nick was braindead. I couldn't believe it. This was it. This was the end. All that hope that I had was false. I went home in shock. I didn't want to wake up to no calls or texts from him. Over the next month, I was constantly surrounded by people. I only listened to music Nick hated for the first week. I just wanted a distraction from all of this. My friends were wonderful thru it all. Finally, when the fifth week rolled around, I felt a depression setting in. It wasn't even when I thought of him. It was when I was at work or school, doing seemingly meaningless work. I stopped eating. I had no appetite. It just seemed pointless. All of a sudden I was alone and it was scary and confusing. Some days were better than others. I could function and smile and be happy, but when it came to coming home, I would drive around the block a few times to avoid going to the place we shared. All his clothing, Bob Dylan posters, political books, and our champagne bottle on the shelf from when we moved in made things worse. Sometimes, I would wake up and it would be the first thought. I would just lay there, depressed. I had dreams that Nick was still alive, but sick. In my dream, I try to convince him to go to the hospital sooner. Other nights I couldn't sleep at all. But one morning, my neighbors below me were blaring their music at 10 am. I was in the shower and I started laughing aloud thinking of how Nick used to hit the floor with a hammer to make them shut up. This made me feel a whole new feeling. Gratefulness that I could have met him and that we shared a love so special. I have new hope for the future. It's the small things that make life worth living and the special people that you meet. Sometimes it's absolutely unbearable, but then I spend time with my friends and realize that there's a lot still in this world that Nick would want me to enjoy. He would want me to look back on those memories and smile and laugh.


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|TÜV SÜD America Inc., a subsidiary of TÜV SÜD AG, is a business-to-business engineering services firm providing international safety testing and certification services. Founded in 1987, TÜV SÜD America has grown to more than 1,000 experts in over a dozen locations throughout the U.S., Canada and Mexico. Operating under the brand names of Product Service, Management Service, Industry Service, Automotive and PetroChem, TÜV SÜD America has partnered with thousands of companies throughout the NAFTA region, assuring product and management systems excellence, and acceptance in the global marketplace.
|Stay informed of the latest management systems and product compliance issues
Product Service division
TÜV SÜD America is a NRTL (Nationally Recognized Testing Laboratory) and SCC-certified, providing a full suite of services, including CE Marking assistance, electromagnetic compatibility (EMC), electrical & mechanical testing, and many additional global conformity assessment services that help companies gain product compliance to enter individual country markets.
Their Medical services unit is the leading Notified Body for a number of EU Directives including: Medical Devices Directive, Active Implantable Medical Devices directive and the In Vitro Diagnostic Directive. In addition, TÜV SÜD America provides FDA 510(k) reviews and third-party inspections, an SCC-qualified ISO 13485 registrar for the Canadian Medical Devices Regulations, EMC testing service provider (60601-1-2), NRTL services, Japanese approvals, and more.
Their EMC (Electromagnetic Compatibility) services unit is a leading provider of EMC testing solutions in the military and aerospace/defense fields. Services include testing to MIL-STD-461, RTCA/DO-160, EUROCAE/ED-14, Def-Stan 59-41, Multiple-Burst and Multiple-Stroke Lightning and HIRF testing up to 9500 Volts/meter.
Their environmental testing unit provides dynamics (vibration & shock), acoustic, climatic and fluid dynamics testing from our accredited labs, simulating the most hostile environments.
Management Service division
TÜV SÜD America is an accredited Management Systems Registrar in the U.S., Europe and Asia, providing ISO 9001, ISO 14001, ISO/TS 16949, AS9100, ISO 13485, TL 9000, OHSAS 18001, ISO 27000, SQF, ISO 22000, Supplier Auditing Services and ESD S20.20 certification services.
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TÜV SÜD America provides a variety of global conformity assessment services for industrial markets, which include consulting, third-party inspection, material testing, inspection & certification, design reviews, pressure equipment testing services, type approvals and Notified Body services for pressure equipment manufacturers and materials producers seeking to export product to the European Community.
Automotive division
TÜV SÜD Automotive provides engineering services for the automotive industry in all fields of motor vehicle technology. From locations worldwide, TÜV SUD Automotive offers a wide range of services including engineering, testing, battery, consultancy and homologation.
PetroChem Inspection Services, Inc.
PetroChem is a leading provider of Non-Destructive Examination and Testing services (NDE/NDT) for the petrochemical and other process-related industries. Employing nearly 400 employees, the organization is headquartered in Pasadena, TX.


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Unless the context in which used clearly requires a different meaning, as used in this article: (a) "Basic public health services" means those services that are necessary to protect the health of the public and that a local board of health must provide. The three areas of basic public health services are communicable and reportable disease prevention and control, community health promotion, and environmental health protection;
(b) "Bureau" means the Bureau for Public Health in the Department of Health and Human Resources;
(c) "Clinical and categorical programs" means those services provided to individuals of specified populations and usually focus on health promotion or disease prevention. These services are not considered comprehensive health care but focus on specific health issues such as breast and cervical cancer, prenatal and pediatric health services and home health services;
(d) "Combined local board of health" is one form of organization for a local board of health and means a board of health serving any two or more counties or any county or counties and one or more municipalities within or partially within the county or counties;
(e) "Commissioner" means the commissioner of the Bureau for Public Health, who is the state health officer; (f) "Communicable and reportable disease prevention and control" is one of three areas of basic public health services each local board of health must offer. Services shall include disease surveillance, case investigation and follow-up, outbreak investigation, response to epidemics, and prevention and control of rabies, sexually transmitted diseases, vaccine preventable diseases, HIV/AIDS, tuberculosis and other communicable and reportable diseases;
(g) "Community health promotion" is one of three areas of basic public health services each local board of health must offer. Services shall include assessing and reporting community health needs to improve health status, facilitating community partnerships including identifying the community's priority health needs, mobilization of a community around identified priorities, and monitoring the progress of community health education services;
(h) "County board of health" is one form of organization for a local board of health and means a local board of health serving a single county;
(i) "Department" means the West Virginia Department of Health and Human Resources;
(j) "Director" or "director of health" means the state health officer. Administratively within the department, the Bureau for Public Health through its commissioner carries out the public health function of the department, unless otherwise assigned by the secretary; (k) "Environmental health protection" is one of three areas of basic public health services each local board of health must offer. Services shall include efforts to protect the community from environmental health risks including, inspection of housing, institutions, recreational facilities, sewage and wastewater facilities; inspection and sampling of drinking water facilities; and response to disease outbreaks or disasters;
(l) "Enhanced public health services" means services that focus on health promotion activities to address a major health problem in a community, are targeted to a particular population and assist individuals in this population to access the health care system, such as lead and radon abatement for indoor air quality and positive pregnancy tracking. Enhanced public health services are services a local health department may offer; (m) "Local board of health," "local board" or "board" means a board of health serving one or more counties or one or more municipalities or a combination thereof;
(n) "Local health department" means the staff of the local board of health;
(o) "Local health officer" means the individual physician with a current West Virginia license to practice medicine who supervises and directs the activities of the local health department services, staff and facilities and is appointed by the local board of health with approval by the commissioner;
(p) "Municipal board of health" is one form of organization for a local board of health and means a board of health serving a single municipality;
(q) "Performance-based standards" means generally accepted, objective standards such as rules or guidelines against which a local health department's level of performance can be measured;
(r) "Primary care services" means health care services, including medical care, that emphasize first contact patient care and assume overall and ongoing responsibility for the patient in health maintenance and treatment of disease. Primary care services are services that local boards of health may offer if the board has determined that an unmet need for primary care services exists in its service area. Basic public health services funding may not be used to support these services;
(s) "Program plan" or "plan of operation" means the annual plan for each local board of health that must be submitted to the commissioner for approval;
(t) "Secretary" means the secretary of the state Department of Health and Human Resources; and
(u) "Service area" means the territorial jurisdiction of the local board of health.


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Mandideep is a Industrial area established in year 1975, is situated near Bhopal, 25 KM away from state capital of Madhya Pradesh. Mandideep Industrial Area contributes more than 6500 crore of Industrial Production, Rs. 1700 crore of Export and more than 45000 no. of employment.
Mandideep is a city and a municipality in Raisen district in the Indian state of Madhya Pradesh. As of 2001 India census, Mandideep had a population of 39,898. Males constitute 56% of the population and females 44%. Mandideep has an average literacy rate of 65%, higher than the national average of 59.5%: male literacy is 73%, and female literacy is 56%. In Mandideep, 18% of the population is under 6 years of age.
Industrial Area
It has an Industrial area, just south of Bhopal capitalising on the proximity to Bhopal.
Raisen District is a district of Madhya Pradesh state of India. The town of Raisen is the district headquarters. The district is part of Bhopal Division.
It is situated between the latitude 22 47' and 23 33' north and the longitude 77 21' and 78 49' east and is bounded in the west by Sehore District, in the north by Vidisha District, in the east and south-east by Sagar District, and in the south by Hoshangabad and Sehore districts. Raisen District takes its name from massive fort. This fort is built on a sandstone hill, at the foot of which settles the town. The name is probably a corruption of Rajavasini or Rajasayan, the royal residence. Raisen District lies in the central part of Madhya Pradesh. The total area of the District is 8,395 Sq. Km., which contains the 1.93 % of the State's area.
Mandideep is located near Bhopal, the capital of the state of Madhya Pradesh. Bhopal is a beautiful city of lakes and hills located at a distance of about 700km to the south of Delhi. There are convenient flights and trains from Delhi and Mumbai to Bhopal. Mandideep has more than 600 factories. Some of the largest names of Indian industry have their units in Mandideep.
Time zone
39,898 (2001)
• Pin code
• 462040
History of Mandideep
In 1983, Mandideep housed 49 industrial units. Looking back through history, Mandideep situated in close proximity to Bhojpur's Shiva temple was surrounded by a big lake since the 10th century. In the 15th century, Sultan Hussein Shah of Malwa attacked the Bhojpur temple and demolished the Bhojpur dam, which kept releasing water for 30 years. Mandideep village came into existence when the land dried up. In the last three decades, Mandideep has touched new heights of industrial success.
Initially, the pace of development was slow due to lack of infrastructure. Hindustan Electro Graphite (HEG) was the first industry to light the lamp of industrialization in Mandideep.
In the 1980s, several changes helped industrial development. The Madhya Pradesh State Industrial Development Corporation completely aided by the state government was formed with the objective of promoting development of industries in this region.
About 660 plots were developed for various purposes. Besides, 45 kms of 'pucca' roads and a 42 km-long water supply system were developed.
In last three decades, Mandideep has touched new heights of industrial success. Thirty years after industries first came up in Mandideep the industrial suburb of the state capital is being compared with leading industrial centers.
Either to a sleepy village in Raisen district, Mandideep, which started on an area of 560 acre, is now sprawled across 2,000 acre. Mandideep has three sub-stations of 250 MW each at present along with other facilities.
Mandideep houses more than 400 industrial units belonging to reputed companies including that of Proctor and Gamble, HEG, LUPIN Laboratories, TAFE Tractors, Godrej Foods, Insulator and Electrical etc. It boasts of a total investment of Rs 4,000 crore.
The industrial area boasts of an export turnover to the tune of Rs 1,700 crore and revenue of Rs 1,400 crore in form of central excise, commercial tax, income-tax, property tax, etc.


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Time for a comparison as promised in the post about the new GeForce GTX 650 Ti Boost GPU that looks very promising as an affordable solution for people willing to play in stereo 3D mode at a resolution of 720p. The direct competitor for the GTX 650 Ti Boost is the Radeon HD 7790, so I took one of these cards and compared the results it provides in 720p stereo 3D mode using the latest TriDef 3D driver to the results of the GTX 650 Ti Boost using 3D Vision. I've also compared both cards in 1080p 2D mode with the same games, because the Radeon HD 7790 is also a good option for people with tighter budgets that want to be able to get high detail levels at 1080p resolution in non-stereoscopic 3D mode, just like the GTX 650 Ti Boost is.
Starting with 720p stereoscopic 3D results, you ca clearly see that apart from Far Cry 3 where the average framerate is very close for both cards and the game Tomb Raider where the Radeon is slightly faster in the other games the GTX 650 Ti Boost performs significantly better. The reason for that is the fact that the TriDef 3D driver is not as efficient performance wise as compared to the 3D Vision, so you might want to consider a slightly faster Radeon video card than the 7790 for playing in stereoscopic 3D mode at 720p resolution or sacrifice some of the detail levels and go for lower settings. Another interesting things that has turned out is the weird out of video memory message that we've got when trying to run Resident Evil 6 with TriDef on the Radeon HD 7790, apparently the 1GB video memory that the card had on-board was not enough.
Moving to 1080p non stereoscopic 3D tests the results turn out completely opposite than what we've had for the 720p stereo 3D tests. Here in Crysis 3 and Dead Space 3 the Radeon is a bit slower, the average framerate is the same in Bioshock and in the other three games the Radeon is faster. SO while both the GeForce GTX 650 Ti Boost and the Radeon HD 7790 offer very good performance for playing games in 1080p resolution with high detail levels, the Radeon might be the slightly better choice. At the same time the GeForce GTX 650 Ti Boost is definitely the better choice for stereoscopic 3D gaming at 720p resolution.


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This was easily the most dramatic and fantastic show of the season.
Wednesday, March 7, 2012
147 comments
some amazing prints and beautiful luggage (as always.)
I love the shape of the shoes too.
I like the concept of having the porters following the models. Very Downton Abbey.
Everything's just so fabulous about this show! Their outfits, the decorations, the bags….
Amazing! The IDEA is simply great and so are the collection!
What a gorgeous collection of fantastical, magical, lovely clothing. I remember I read a lengthy feature on Vuitton's original creations and venture into design and I gained so much respect for him and his company. I wonder if part of this display was inspired by the movie 'Hugo' in a French train station? Love a great story to accompany great design.
This is the Downton Abbey effect; nostalgic reimaginings of a lost age. These are beautiful clothes, fabulous design and a wonderful eye for color and print and yet do they veer on fancy-dress costume?
The Louis Vuitton Collection F/W is fabulous! The colors are great!
Love the patterns !!!
stephaniehadrath.blogspot.com shbyhadrath.blogspot.com
Very nice concept:)
All those prints are fantastic ! That collection is so beautiful!
I love the idea of the men carrying the ladies' bags ! It's genius !
Amazing! I think I like the men better than the models here!:)))
That is quite the statement coming from you…but totally true!
An amazing show & such lust worthy bags!
Hooooooly moly!!! In love with the embellishments!
Love the runway concept!! great rich Fall colors
so classy! amazing stage setup!
This show was magical, absolutely theatrical, beautiful! Marc Jacobs does it again!
yes, I too like all that the models are wearing, now if Marc can do something for the bellhops
looks more than great!
Love the highly-attentive baggage handlers. Great idea, LV.
Street Lovely
OH MY WORD. This is the most fantastic show I've seen in SO long! Absolutely brilliant…theatrical, without flirting with cliche. Amazing.
C'est magnifique!
oh my goodness! what a dream. the bag porters are just perfect
LOVE LOVE LOVE the hats. I'd love to pull of that look.
G O R G E O U S! Stop by & see my Las Vegas trip.
looks like any Marni show
Gorgeous collection and really nice prints! Also a great setting and the guy that's walking behind the models with their bags is a great find!
Eye candy: sumptuous brocade and jacquard…Marc Jacobs is on a long, strange "A la Recherche Du Temps Perdu" trip with those Edwardian hats.
Amazing prints, lovely pics. http://www.hotcelebration.com
The layers! The gems! The prints and brocade. This was a great couture show with so many inspiring pieces and outfits.
And the porters are adorable; I love that they didn't hire male models, just normal men!
very "fin de si├Ęcle" j'adore.
I feel it is very similar to the Marc Jacobs show though – no? The big hats, the colours, the bulky buttons as well as the cuts…
Beautiful collection, though for some reason I'm totally distracted by the expressions on the faces of the bell-hops….
Amazing collection, the colours are really awsome!
My elder sis and I have recently opened our own fashion blog, when you have the chance please have a look at it.
xxx Giorgia
i am mad for every single pic of this post!
this is superlative!
Amazing! The idea of the show is great!
easily one of my favorite shows this season! loved the presentation.
I can see that it is very dramatic but a little TOO dramatic for my tastes. I'm sure it will 'translate' well! Think that's what they say.
Fantastic concept!
That show looks incredible! Such an original idea for a catwalk. And of course, I love the dresses.
Absolutely gorgeous!!!!! Wonderful show. BRAVO Marc Jacobs!
This is inspiring!
Sartorialist……you are the best!
Very 'Downtown Abbey' 1910-1918 look. I love the shoes, which, apart from the height of the sole are also redolent of that time with square toe and foot shaping. The patterns are astounding and have taken real skill to balance it seems to me – with splashes of block colours in the hats (also true to time) and bags – specially like the yellow. I also like the almost Asian idea (French Vietnamese?) of pants beneath the calf length skirts.
very crazy!! drama is for sure, it reminds me a little of the movie "hugo"…parisian cafe culture…
The best show I've seen this season. Real Boss type.
Just amazing !!
Oh man, I feel like I just died and went to "Funny Girl" heaven. This is like the scene where Barbara Streisand gets off the train and runs into Nick Arnstein EXPLODED all over "Hugo". I'm in love. I'm going to be dreaming about this coat silhouette (the ones with the curving hi-low hemlines) for days.
This collection immediately reminded me of the novel Anna Karenina.
I loved the feel of this show. It was so unique and made their new clothes stand out in my mind.
What a cool show! I love the outfits! The shoes are super cool as well, and don't get me started on the bags!
Absolutely.Love.It !
I love this collection. Marc Jacobs is a genius!!!
I'm sure the narrative employed for the show by Luis Vuitton really had the front row on the edge of there seats.
Did the 'will they / won't they' relationship develop any further between handbag lacky and model?
or did the train depart on a love which just wasn't meant to be..
Find out next season / episode ;)
I might be the only one on here that dislikes this collection. Those hats are horrible too. They remind me of paper hats you make at a earth- hippie festival
Amazing scenary, amazing looks. Love it!!!!!!!!!
Check my new post
i'm just so in love with the luggage!
and the theatrics!
<3 Lindsey
Wow! Reminds me so much of the Musee d'Orsay, the train station turned museum, also with a beautiful clock hanging in the centre of the wall!
amazing looks
do they come with the bellboys
I love love love!
Like the show concept in terms of the train station feel…not too crazy about the actual designs though. Feel like the proportions are a little off, and the hats are giving me "mad hatter" vibes – and not in a good way.
Oh well. Can't win over them all, no? :3
– Miz L.
I love the luggage but feel this show is a sort of ripoff a little of Galliano's train station runway show for Dior.
A vintage lover's dream!
Gotta love those purses! Yea baby! LOL!
The inspiration seems to come from children's literature: Dr. Suess(Cat in the Hat hats) and Ernest Shepard's illustrations of A.A. Milne's "When We Were Very Young." Numbers 8 & 9 remind me of James James Morrison Morrisons' mother.
The 60′s are back and ain't they gorgeous. Now everybody go out and have fun now, you hear
While many of the clothes are certainly beautiful, it's hard for me personally to get past how distasteful I find the presentation concept. The fetishising of excessive consumption, played out literally by having so many bags you need a subservient man to carry them all, is revolting. I thought we were over the whole It bag thing. This show is about spending and spending and still not having enough, aimed at women who have more than most would dream of in a lifetime. For shame. And for those who would defend it as fantasy and a work of the imagination, this is a business. And they know their clients.
love the stage setup, classy looks!
Is it just me or is there a strange costume things happening here? I appreciate the pomp and circumstance here but I am not really loving these looks.
luv luv luv…it's chic mad hatter ~
The shimmering brocades are exquisite with the shorter pants. But the shoes, oh the shoes, make the show perfect!
is it Ken Russel┬┤s Alma Mahler…?
YES YES YES, Alma was wearing that…"costume?", at the station before catching the train,
he stoled the whole concept, Naughty Boy!!!!
So glamour!!! Luis Vuitton, one of my favorite fashion brand!!! I especially love its bags!!!
I agree with M, the whole collection reminds me of the Marc Jacobs collection only a little more streamlined. I think even a couple of the fabrics are the same…
I did love the concept of the train and the porters to pay homage to the famous Louis Vuitton luggage line.
Wow! C'est formidable!
I love the fabrics and the theatrical-ness of the clothing but let's get real. Who in the hell is gonna wear that mess? I guess if one were going to a Cat In A Hat convention.
Truly amazing collection, and the photographs are just out of this world… hard to believe this was on a runway. The whole look and feel of this dramatic show comes alive trough the images posted. Wow…
just like prada/miu miu you see a direct relation between LV and Marc Jacobs, as there were done by 1 peson, but beautiful
gets better and better
I see some continuity with the Marc Jacobs collection/show (though the hats definitely work better here), and once again, I can see key elements of the collection translating beautifully. The third and next-to-last coats, however, are pretty darn sublime just as they are!
Unbelievable! Such a clever idea for the show!
God what an amazingly fabulous collection! Im in love with it all.
Amazing show!!
So impressive ! Love the bags and the prints
http://www.tripsandtreasures.net – new outfit post
there's a whole lot of Marc Jacobs in this collection…
Rebecca – agreed. Crazy culture we live in.
hm. this show is clever product placement indeed. as far as the clothes and the fabrics are concerned: marc jacobs is a talented magpie and i really like a lot of what he's done, but these last f/w collections are a bit much and just don't do it for me. they lack sensitivity, or poetry.
Is that uniform from LV too? :P
Such attention to detail. A wonderfully stunning posting!
amazing show!!!!
great show! Love the idea of boy carrying the bags after model!
love the scene, oh good I love men in uniforms!
muah x
Absolutely love the layering, beauty and power in most of the outfits. Truly stunning line up.
great work of all Louis Vuitton team, these is surprising! thanks for sharing with us Mr. Scott
love from http://benyricardo.com/itsme!/
i onestly think LV show was the worst show of paris fashion week. it was a mess. a complete disaster.
all the clothes look so unatractive, the silhouette was old, as the concept, wich before the "abbey thing" is actually more "the hours" virgina's wolf 2002 movie. those velvet puffy hats were the most ugluy thing that came out from paris fashion week. those coat shapes were just wrong. the collar was old and heavy, the prints were messy, the embroideries and the buttons looks cheap. and the pump edgy shoes were horrible. too big, too sharped.
i want to know if there just one woman in the world who look sexy and fresh with that garments on her.
I am captivated by this runway presentation. Having male models as porter carrying the bags gave fresh look on runway! I am totally in love with the bags in this collection!
Beautifull prints!…Amazing as always!
OK, so I would never wear any of these clothes or derivatives of them – they look so heavy and bulky (makes me feel hot, itchy and, I dunno, 19th-century-imprisoned-by-my-wardrobe just looking at them) – but what a beautiful/striking show.
what's with marc jacobs and mad hatter hats?
Wow. Every piece is stunning, and the presentation perfect.
I love the theatrics of the runway show and the detailing of the jackets
I agree with Rebecca
I LOVE the train and the idea of the lovely helpers carrying their lovely bags ;) so creative and the clothes are to die for. A bit of a world away it seems, Stockholm Street Style is all about simplicity, yet big bold statement pieces at the same time ! Take a look at my Stockholm Street Style blog: http://www.johannasthlm.blogspot.com
Such an adorable concept! It feels good to have a man behind you carrying your bags and this runway made me feel good!
Personally it leaves me cold. I found that show very tacky and tasteless
Rebecca is applying dialectical materialism (consciously or not) to her analysis of a luxury goods brand…makes me wonder if Susan Sontag wrote about high fashion; I recommend her book, On Photography.
everything feels so labored in this collection. i agree with rebecca on this collection as well, i heard backstage some of the fabrics cost over thousands per yard and the fact that marc jacobs uses them in such an appeasing way is just embarrassing and insensitive. he has an abundance of resources but still refuses to push fashion forward only offering predictable looks through the silhouettes, cuts and styling. it doesn't help that all his collections recently have come across as complete knocks offs of prada and marni pieces, how can no one else see this? and yet people still compliment him on his "revolutionary concepts and presentation skills". his designs are not up to par with likes of forward designers such as balenciaga, chalayan or prada.
finally it annoys me that he has taken the editorial approach to designing as well. the clothes are so unflattering with hideous proportions but photograph well. but once you see them on a hanger it's a different story.
oh lordy be!… THE HATS!!! *sigh*
No, Sandra, you're not alone. This collection looks like the clothes for the witches in the Harry Potter movies. I don't think the lines are flattering and the hats look like old, limp balloons. Colors and textures are cool, but design is too costume-y for me.
Yey for the millinery! Love the sapphire blue hues. Delicious!
Jenelle. xx
These models, their shoes and outfits are awesome!
The expression on the porter's faces is hilarious. Like "what's this all in aid of then???"
Beautiful photos…..magical….could be a movie
Huge hats… again! How is it possible that designers come up with similar ideas (oversized hats, similar colours, patterns, silhouettes, fur) within the same season? I homestly don't understand how that works. Sartorialist, please explain! Is there some kind of secret annual meeting for fashion designers??
i have to say, i saw the shots vogue.com posted for this and for miumiu, and i outright rejected them in my head – simply disliked them. but something about your photos… I automatically saw the very same outfits as dramatic, artistic, and creative. i dont know what it is, but you just seem to capture them in just the right way; it makes them so much more fashion-y than just seeing the clothes.
Gee, Rebecca, I wish I could find a porter (whom I would tip) to carry my bags, as I am only a little person and have difficulty. In any case, porters have a respectable job and a nice uniform. What's wrong with that? Should we go to the kitchen and get our own lunch next time we dine out?
This collection is beautiful, but there is something very costumy about it. I think fashion should draw a real line between itself and the theatre and I find more and more it does not. Marc should design for the ballet sometime. I could picture "Cinderella" (Prokoviev) designed like this.
I love how blue eyes of the boy on the second from the last picture steal the show. Fashion is clothes and accessories but on top of all it is about people. People beauty, charm, personality…
What I love most about this show are the porters with the gorgeous luggage!
I simply L O V E everything in these pictures. LV has been doing some great shows lately.
Thank God there's someone left in the world with balls big enough to do this stuff–literally dreamy!
Love your photography! I am also in photograpy and and have a love for fashion. (My fashion blog launches 3.25.12). The L.V. line is amazing. Very inspiring. It's great to see patterns, texture and bold hues. People should not be afraid to define their style and wear those platforms!
What's with the Cat in the Hat hats?
a theatrical spectacle, gorgeous bags yes, but for me, those clothes are 'emperors new clothes' hideous. The collars, the buttons, the shapes, those shoes! -awful. Fantastical narrative, my fair lady on acid, (with some Carol Brady, Shirley Partidge thrown in) but unwearable. Or if I saw someone wearing any of these looks I'd feel they'd drunk the koolaid and were desperate victims of 'fashion'. Maybe its just me…I can tell the experience of the show was transporting but the clothes the clothes the clothes are too contrived and just ugly.
Again, I agree with Rebecca, Ludo, Dominic,Sandra, Sarah, the clothes are desparately unflattering. Period. "the emperors new clothes" syndrome. Also no one seems to be talking about the pink polo dress with Louis xiv pumps and purse outfit Marc wore to his celebration? Besides looking foolish, i saw: Warning Signs -potential train wreck in the train station/Gare Galiano. I hope not. I don't say those things to be catty or mean, just an observation…
I just love how those porters seem to be 'soldiering on' with grim determination. Some of the expressions are like: "How did I get myself into this?"
To have someone just walking after you carrying your bags…is at least funny… I liked the way the brand focus in its ideal customer, but maybe there are some clients who don't sympathize with this "madame" thing…
I loved all bright and beautiful colors and amazing fashion styles..! I want to say two words absolutely glories..:)
The show really updated the idea of defil├Ę. Smoke at the end of the catwalk, the clock beating the hour and … a real size train enter the hall.. and then you are sent somewhere back in the past waiting for your lady arriving at the train station from the Orient, maybe in the '30..that was amazing. I was there. Believe I felt guilty I didn't have my hat, my wooden stick and my gloves, ready to welcome her..
I want to take issue with the objections to fabrics that cost hundreds of dollars a yard. What is wrong with that? These gorgeous fabrics are handmade by real artisans. I cannot afford them but I'm glad that a few can. And because a few can, the talent and skill of these artisans is not lost.
Thank goodness that not everything is manufactured cheaply by poor drudges in factories. And I don't just mean clothing.
It`s awful, a circus. Such a waste of extremely expensive materials and labour.
Not a fan!
E' retro di sapore… molto bello!
I adore it all, the clothes, the hats, the train, clock and porters, like stepping into Hugo. For those who are upset about the expense–think of all the people employed in creating this show, from the artisans–fabric makers and designers, seamstresses, shoe makers, milliners, to the men and women who constructed the sets, hung the lights, set up the chairs. Isn't there something to be said for keeping alive admirable vanishing skills, and for wild beauty?
Amazing patterns and shoes and of course the bags are brilliant! Love Louis Vuitton!
Trains are too dirty to wear anything like this…. That is a period long gone, and never to return…. No one dresses well to fly, either. It's shameful. Clothing is cute, but, really? Who wears this?
I loved these all bright and beautiful designs with great color combinations i like all specially the lost one, thanks for sharing with us..:)
Womens Online Store
amazing bags !!!!!!! i love the concept, the idea, the scenography !!
Now THIS is some good fashion.
Makes me want to take the Orient Express … Jacobs is a genius
L'ensemble des ¤üost ĐĽont r├ęellement attractifs
Nice concept. outfits and bags are fabulous.
where I can buy these clothes?
EverythingÔÇÖs just so fabulous about this show! Their outfits, the decorations, the bags http://www.ritoot.com/blog/


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Michelle Obama Jokes About Bangs, 'Mid-Life Crisis'
(WASHINGTON) -- First lady Michelle Obama joked in an interview set to air Wednesday that her now-signature bangs weren't a fashion statement but a result of a "mid-life crisis."
"This is my mid-life crisis, the bangs," the first lady, 49, said in a preview clip for Wednesday's Rachael Ray Show. "I couldn't get a sports car. They won't let me bungee jump. So instead, I cut my bangs."
The first lady made fashion waves when she first sported the bangs in a photo uploaded to her Twitter account on her birthday last month, just before inauguration weekend, and the president quickly admitted he's a fan of the new hairdo, saying it was the "most significant" event of the weekend.
"I love her bangs," Obama told supporters at an inaugural reception at the National Building Museum the night before the inauguration. "She looks good. She always looks good."
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