text:
Pucker up: Meyer lemons are an addicting addition
Pity the poor cook who has no dirt. My five-blocks-from-the-beach home is short on the stuff. With a goal to use every inch of growing area, one year ago we took out the oleander tree in the 5-foot plot that faces the city alley behind our abode.
In its place we planted a Meyer lemon tree. I thought it would bolt up quickly, so I opted to save some cash and bought an immature plant. It hasn't grown even one inch in height, but the branches sag from the weight of its bountiful orbs. Only 1-foot high, it has produced more than 30 juice-packed lemons.
I suspect that everyone who owns a Meyer lemon tree cherishes each and every lemon. Often more spherical than the more common Eureka variety, Meyers have thinner, brighter yellow skin. Their scent and flavor hint of sweet tangerines.
They are a hybrid (a cross between a lemon and either a common orange or a mandarin orange), brought from China to the United States in 1908 by Frank Meyer, an employee of the U.S. Agriculture Department.
For years they weren't easy to find outside California, and most seemed to be grown in residential settings. Now they're grown commercially in Texas and Florida as well as in California, in large part because of the demand created by chefs.
Alice Waters, owner and founding chef of the legendary Chez Panisse Restaurant in Berkeley (she is often described as the mother of California cuisine and the creator of The Edible Schoolyard program), has touted Meyer lemons for use in everything from drinks to desserts, entrees to sauces to salads.
"To those who have never tasted them," Waters writes in "Chez Panisse Fruit" (Harper Collins, $34.95), "our recurring insistence on Meyer lemons may seem like an irritating California eccentricity, but I don't know many people who have tried one without getting hooked.
"Ripe Meyer lemons are sweeter than other lemons, and the skin has a distinctive floral fragrance. ... At the restaurant we add a squeeze of lemon to a dish almost automatically, to boost flavor or balance acidity."
Meyer lemons can be used in many of the same ways as ordinary lemons, but you need to make allowances for their lower acidity. So when substituting Meyer lemons for more common Eureka lemons, if the recipe calls for zest, add a little more Meyer zest. And if a pucker-producing pie or tart is what you're aiming for in, say, a lemon meringue pie, you might want to use juice from a Eureka – or use half Eureka and half Meyer.
The following recipes are some of my favorite ways to use Meyer lemons. If you don't have a tree, look for Meyer lemons at farmers markets. Some supermarkets with large specialty-produce sections have Meyers intermittently throughout the season as well. Or feel free to use Eureka lemons; the dishes will still be vibrantly delicious.
Recipe: Fish Fillets with Capers and Meyer Lemons
Recipe: Steak with Spinach, Parmesan, Grilled Lemon, and Olive Oil
Recipe: Iced Little Lemon Drop Cookies
Recipe: Shaker Lemon Tartlets
Meyer Lemon 101
Commercial availability: Domestic Meyer lemons available mid-October through March (sometimes extending through May). Peak of the season is December to February. New Zealand imported Meyers available September to early October.
Buying: Choose lemons that feel heavy for their size with brightly colored skin. Refrigerate up to three weeks. Or store at room temperature up to one week. Look for Meyer lemons at farmers markets and some supermarkets with large specialty-produce sections.
Zest: The zest, the colored portion of the skin without the white pith that sits below it, is full of aromatic oils. If using the zest, remove it from the washed fruit when it's whole (before juicing), either with a vegetable peeler, grater, zester (a tool with several tiny holes that cut threadlike strips of zest) or Microplane.
Juice: Room-temperature lemons yield more juice. Rinse thoroughly. To produce as much juice as possible, before cutting, roll lemon on a flat surface while pressing firmly with palm of your hand.
Grilled: Grilling lemons gives the juice an alluring smoky scent; cut lemons in half, brush cut surface with olive oil and grill them cut side down on a clean grate, until dark grill-marks form, about 2 minutes. Be sure to allow lemon to cool before squeezing over the dish.
Simple Vinaigrettes: Make a lemon-spiked vinaigrette right on the greens. Use greens with "attitude," such as arugula or mesclun (mixed baby greens). Coat them lightly with extra-virgin olive oil; toss, then squeeze on a little Meyer juice. Toss again, season with salt and pepper, then top generously with thin shavings of Parmesan cheese. The perfume of that orange-kissed lemon makes those greens sing.
Too much? Fill flexible plastic ice-cube trays with juice. Frozen juice cubes are a handy, year-round source of lemon juice. Once frozen, the cubes can be popped into large, zipper-style plastic freezer bags.
If freezer room permits, cut lemons in half, hollow them out and freeze them in zipper-style plastic bags. They make great containers for individual servings of lemon sorbet or ice cream.
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'News of Interest to Colored Readers,' 1927-1928
A ten-month column from the Alexandria Gazette written by African-American Estelle Lane about events in her community in Alexandria, Virginia.
Chronicling America: Historic American Newspapers
Search and view newspaper pages from 1880-1922 and find information about American newspapers published between 1690-present. Sponsored jointly by the National Endowment for the Humanities and the Library of Congress as part of the National Digital Newspaper Program (NDNP).
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subset: commoncrawl


text:
Integrate: Is it correct if i do this: and then solve? and then let u = 8-6x and du=-6 then the answer comes to
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Hello, It's all correct
thank you, here is another one (I have a test on Friday so I'm trying to do as many problems as I can) Integrate: I let u = 6x and du = 6dx the answer I end up with is:
Hello, And what about integrating the constant ? The integral of 1 is x, the integral of 3/4 will be... But according to your answer, would the integral rather be ? In which case, it's correct - again
oops no you were right I did it wrong but thanks for pointing that out
the answer should be: correct?
No, it looks like you forgot du=6 dx Otherwise, it's correct.
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text:
I've come across a site with a URL structure I've never seen before. Are these search engine friendly URLs?
It just strikes me as an odd way to construct URLs.
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Search Engine Friendly Url Structure?
Started by
, Dec 02 2011 10:31 AM
3 replies to this topic
Posted 02 December 2011 - 10:31 AM
Posted 02 December 2011 - 11:08 AM
Do those each go to separate pages?
If so, it's not that unusual. You're thinking of them as keywords, but they're likely just describing the page.
If so, it's not that unusual. You're thinking of them as keywords, but they're likely just describing the page.
Posted 03 December 2011 - 06:20 AM
Are these search engine friendly URLs?
Yes and they are a damn sight more "server friendly" than so-called "SE friendly" URIs
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text:
Do you want to make Ohio.com your homepage?
XChuck sent me a note overt the weekend saying he is doing OK after brain surgery performed Friday. He told one friend recently that "The good news is no long range damage to my voice or motor skills, at least not so far. ... Bad news is no apparent increase in math skills." He has posted a more general note to listeners here.
Below is Collins's explanation of what happened, from his blog, where he plans to post more about his progress.
Maria's eyes were like twin moons around planet Fear. The one on the left was the scientist, strong and confident; the one on the right was the woman, astonishingly sympathetic. They hovered over my bed and waited for their time to shine in this night I was entering. She knew it was going to be a difficult message to deliver, but she had done so, or something very much like it, for more years than her youthful face belied.
"We found something…suspicious." Really, suspicious? Part of my life for the last dozen years has been steeped in finding the suspicious and making it fun, exciting, enticing; that has been my goal anyway. But success has eluded me so far. Oh, I got the work done, tried my best to find the training and understand the craft, and in that regard I'm pleased with the results. But if the idea of moving my craft from mind to market is important (and it is) then I have a long way to go.
Maybe we'll talk about that later. Maybe not.
Dr. Maria Crespo, "just Maria, I hate titles," had efficient features, just enough to support those big hazel eyes. Her hair was pulled back tightly adding to the no-wasted-space of her face. But her smile was easy and fluid. She wore minimal make up, just a little unnecessary outline to her eyes and perhaps some foundation. I detected no scent from her whatever, unlike Nurse Amanda, pretty girl, tall and dark with a fondness for a sweet and fruity fragrance. Maria's 5 foot 4 inch command of the room was shocking. Nothing mattered other than me and her message. Med student Josh, Amanda, even ER Supervisor Dr. Clark submitted to her management of the room. It was my room for this short stay. I had hoped it would be a lot shorter.
The ordeal started in March of this year, very close to the 35th anniversary of our first marriage, Monika and me. Our life together had been filled with all one could imagine and more. She has proven what a best friend is, and I wish I could say the same. I can say this: I love her more than life itself and I mean that. But what happened has nothing to do with that heart-shaped mark on a calendar or my Monika. But it does have a little to do with Phil. He is one of the few people I have known – and still know – longer than Monika. We found ourselves working together again seven years ago almost to the day. Phil and I were standing in my little studio where I do most of my air work. We were just talking about radio stuff and a little bit of life. Suddenly, and I mean suddenly, the left side of my body sprang a leak and lost 80% of its communication with central control, or so it felt.
I told Phil to stand by, that I might need help. I waited to reestablish contact with the renegade left arm and leg and some of my trunk. Here's where Uncle Charlie comes in. I was named after my father's older brother. I have not been kind to Uncle Charlie's memory. He was not big on kids, my dad's assessment. But dad was big on family and would do anything he could to keep the small and splintered Collins' Clan together. In reality Charlie meant well, but he just wasn't as bright or accomplished as dad and he just could not seem to drink that fact away. It was not for lack of trying. The most vivid memory I have of Charlie is after he had a stroke. He must have been in his early 50's and it was a debilitating, face melting, gait robbing event from which he never really recovered.
The good news is that at an early age I became aware of probably one of the cruelest of the many cardiovascular/neurological diseases. On that day with Phil, there in the studio, I knew what to check to see if I was having a stroke. I ticked off only one of the six on my list. My speech was not slurred, I could see equally well out of both eyes, and though the south side had taken leave, I still had ample strength to get back to my office, have a seat and in a few minutes get back to work. Perhaps it was my playwrights of the naïve or the absurd, but I did no follow-up.
That weekend it happened again. Part of what I do, and I like to think do well, is host crowds of people at community events. That weekend was one I really enjoy, although it is a long night. The first 6 hours all went very well. Then, as I was reading off winning numbers and names, that feeling returned and half my body nearly left the building. This time the odd and very disturbing sensation lasted the whole night and all the next day. At this point any reasonable person would head to the ER, or at least make a soon-as-possible appointment with a doctor. Any doctor!
Not me. I shook it off, so I thought. As you can imagine it came back, several times in fact and with new components like a spoiled child asserting himself. Soon odd sensations in my taste buds and saliva flow accompanied the events, as I came to call them. I would recognize these little visitors, wait until that passed and be thankful when nothing bigger happens, such as passing out, losing the use of my limbs or joining the renegade half and slip into semi-oblivion. It never did, nor did they go away.
One fine June day I was sitting in the beautiful back yard of a friend, a retired cardiologist and writer. Dr. Terry Gordon had become something of a legend in this area, not only because of his immense skills as a surgeon and innovator, but because of the tragedies that had befallen his otherwise rich life. More importantly is how he came to handle these lifemares. Terry listened to my story and almost immediately asked if he could "give a listen." He brought out a cuff and stethoscope and took my blood pressure. It was extremely high. He insisted that I see a doctor right away, a legally practicing doctor.
That set me on a journey toward treating hypertension. The events, it was believed, were Trans Ischemic Attacks, TIA's or mini strokes. BP under control the larger events stopped. But now there were smaller and more frequent events, about three a day. Something was still very wrong.
This human can be a prime example of stubborn nature. I rationalized everything, from a pinched nerve to the way I sleep to my laptop searing nerves in my thigh at the exhaust fan. But there came a point when I knew something was really screwed up.
After walking into the ER in Cuyahoga Falls, I still had the feeling that I was wasting everyone's time, not to mention spending deductable money that we don't have. That's another difficult part about getting sick these days, the first thought that comes to mind is money. There is something inherently wrong about that and before I'm finished here we might get into it a little. I promise, no heavy hands here.
Now we have come to the start of this experience. CT scans exposed a mass in my brain.
I remember reading and hearing all kinds of reactions to the news – news I have not gotten definitively yet – that one can add cancer patient to ones self image. Most talk about the seven stages of pre-grief. They speak of fear and anger in equal doses, then comes acceptance and determination to beat the beast. I don't have any of that right now.
I have an incredible sense of wonder. I wonder what this really means. I wonder if it's going to really mess with my head, my personality, my ability to do what I am doing right now. If the latter becomes reality, then, as one of my favorite characters in The Radio Murders: The Collectors exclaims, "I mays well just go on up to Jesus right now." Those were the words of Mary Lofton, age 92, and a pretty close capture of my mother, Mary Collins.
But here is the odd part: The tumor is in the right parietal quadrant of my brain, in a section that is partially responsible for the motor functions of the left side of my body. It has not yet affected my speech, vision or reasoning. In the words of a PA from Neurosurgery, if you're going to have a brain tumor and you are right handed, best to have it in the right side of the brain. Of course, I'd rather not have it at all.
As part of my recovery and ultimately the Journey, I am going to keep this running account of my feelings, observations and a little about the people I have met along the way. For example Angel just brought me a new remote control for the TV. She is a butch-haired breeze of a woman with sandy features and a quick step. She is very nice and I would not trade Angel for anyone under these circumstances.
The Brain Team is starting to form. First there was Shannon the neurosurgery PA, an energetic red-head who knows how to defuse words like tumor and cancer. She pretty much laid out the plan for how we were going to discover "clues," and tackle the problem one step at a time. Then came Brittany, thin and blond with intense eyes and a fast-paced way of talking that she can stop on a dime to hear Monika or me out. She was the neurology Physicians' Assistant. Right behind her was Ryan, Dr. Lynch. A big man with a "go ahead, try and shake me" kind of face who gave off an air of, the only way I can put it, this is what we have and this is how we're going to beat it. He reminded me of the NFL player who came back to teach high school football, except he was neither, just a guy who loved learning and, "two advanced degrees was all we could afford."
Now the waiting, and it is true, that can be the hardest part. I'm sure there will be much more to say about this pretty dramatic experience. One last person I want to add is my new Internal Medicine doctor. Dr. Miller. Pretty and youthful for her nearly 50 years, brunette hair cresting below her shoulders, with eyes that reflected the sky in the window behind me and actually made it nicer. She actually glowed confidence like most moms of boys (3 of her 5 are boys). That assuredness was not in her necessarily, but in me, Monika and the process. There was not a doubt in her mind that we would come out of this better!
And what about Monika? That is a real story that I will get into next time


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« Labor Day! Labor Day! Break Free, Break Through the Walls of the Status Quo Criminal Class | Main | HEINRICH: Accelerated Drawdown in Afghanistan Needed »
Tuesday, September 04, 2012
Maggie Hart Stebbins: Highlight Of My Time on County Commission
On August 20th, Commissioner Maggie Hart Stebbins issued the following press release.
Leading the Way on Innovative Bernalillo County/UNM Partnership that Protects North Golf Course from Development
I'm thrilled to share with you exciting news of an event that will always stand out as a highlight of my first three years as a Bernalillo County Commissioner-- This past week, I had the privilege of joining Jack Fortner, President of the UNM Board of Regents; UNM President Dr. Robert Frank; and leaders from UNM Area neighborhoods to celebrate an outstanding accomplishment -- the approval of a partnership that will preserve the UNM North Golf Course as Open Space!
Saving the North Course has been a top priority for me from my first day on the Commission and I'm overjoyed that concerned neighborhood and community leaders and UNM Officials worked with me to find a solution that protects the last, best open space in our part of the county.
Both the Board of Regents and the County Commission approved an agreement that allows the County to dedicate $1.5 million from our Open Space budget in exchange for a 15-year conservation easement on the 80-acre North Course property. The County's investment will fund installing a new, more efficient irrigation system, replacing aging trees and landscaping, and improving the very popular pedestrian path along the perimeter of the course.
The University and the County leveraged our respective resources to engage in a partnership that is truly a win-win-win for everyone involved. The 80-acre course provides a host of environmental and recreational benefits -- the new irrigation system alone will save an estimated 20 million gallons of precious groundwater every year, at a time when our water resources are threatened on a number of fronts.
And I am pleased that the residents of District 3, who have faithfully contributed to the County Open Space program, finally have a property that is within easy reach of our neighborhoods.
In these days when partisanship and turf battles seem to paralyze our governments, I'm especially proud of this partnership because it demonstrates how local leaders can do great things for the community when we work together. I hope that it will serve as a model for future collaboration between governments in this region.
Also this past week, at dusk, I paid a visit to the North Course. As I walked my dogs, with the 70-year-old elms arching above me, I joined at least a hundred other residents who were doing the very same thing – children and parents, kids with soccer balls and dogs and runners - dozens of people just like me who were taking advantage of the peacefulness of that open space. It was a beautiful sight and I felt very proud to have had a part in preserving that experience for years to come.
As always, I am grateful for your support that gave me the opportunity!
September 4, 2012 at 09:44 AM in Bernalillo County, Land Issues | Permalink


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For Immediate Release
Matt Jacob
Phone: 202.408.5565
CREW Files FEC Complaint Against Crossroads GPS for Failing to Disclose Donors
WASHINGTON - Today, Citizens for Responsibility and Ethics in Washington (CREW) filed complaints with the Federal Election Commission (FEC) and the Federal Bureau of Investigation (FBI) against Crossroads GPS for violating federal election law by failing to disclose the identities of contributors who — according to Karl Rove — donated $6 million specifically to fund independent expenditures in the Ohio Senate race. Additionally, Crossroads GPS also broke the law by soliciting donations to be spent on the Virginia, Montana, and Nevada Senate races, but failed to disclose the donors.
Read CREW's complaints to the:
CREW Executive Director Melanie Sloan stated, "Karl Rove and Crossroads GPS didn't just skirt around the edges of the law; this time it appears they jumped headlong into a criminal conspiracy."
Federal law requires any outside group that makes an independent expenditure to disclose the donors who contributed to pay for such ads. Groups like Crossroads GPS normally evade this law by claiming none of their contributions were earmarked for a specific purpose. At an August 2012 fundraiser, however, Rove said an anonymous donor gave Crossroads GPS $3 million specifically for the Ohio Senate race, and told Rove it was a "matching challenge" dependent on the group raising another $3 million for the race. Crossroads GPS ended up spending $6.36 million on independent expenditures in the Ohio race, but did not disclose any donors in nine reports the group filed with the FEC.
At the same event, Crossroads GPS showed attendees advertisements it had made for five other Senate races, after which former Mississippi Gov. Haley Barbour and Crossroads GPS president Steven Law asked the donors for more money, presumably to pay to run the ads or others like them. Crossroads GPS spent another $17.4 million in these races after the fundraiser, but did not disclose any donors in 31 reports it filed.
Crossroads GPS told the FEC in a 2011 letter it understood the law, and promised to identify all donors who made contributions for independent expenditures in specific races. Because the violations were deliberate, they are subject to criminal as well as civil penalties.
Sloan continued, "Rove and his cadre were free to waste hundreds of millions of billionaires' money in their ultimately futile quest to see more Republican senators elected. They were not, however, entitled to break the law to do it. There is ample evidence Crossroads GPS, Rove, Barbour, and Law illegally raised money to fund specific ad campaigns but didn't disclose the donors. The only question now is whether the feckless FEC and the feeble Public Integrity Section are going to do anything about it."
Citizens for Responsibility and Ethics in Washington (CREW) is a nonprofit 501(c)(3) organization dedicated to promoting ethics and accountability in government and public life by targeting government officials -- regardless of party affiliation -- who sacrifice the common good to special interests. CREW advances its mission using a combination of research, litigation and media outreach.


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11 February 2014
11 February 2014 Last updated at 15:30 GMT
The Prime Minister has denied flooding in Somerset took longer to "get on the government's radar" than the situation in the south east of England.
The Somerset Levels first flooded at the end of December and a major incident was declared by Somerset County Council on 24 January.
Speaking at the silver command control room in Taunton, David Cameron said that "as soon as the problems came in Somerset" a response was put in action.
He was also challenged about why the government did not sanction spending an extra £1m to dredge rivers on the Somerset Levels.


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Lemon-Lime Layer Cake (Adapted from CL)
For the cake
2 1/4 cups all-purpose flour
1 1/2 teaspoons baking powder
1 teaspoon baking soda
1/2 teaspoon salt
8 tablespoons butter, softened
1 1/2 cups granulated sugar
2 large eggs
2 large egg whites
1 tablespoon fresh grated lemon zest
1 tablespoon fresh grated lime zest
1 tablespoon fresh lime juice
1 1/3 cups low-fat buttermilk
For the icing
8 ounces cream cheese, softened
1 teaspoon fresh grated lemon zest
1 teaspoon fresh grated lime zest
1 teaspoon fresh lime juice
2 1/2 cups confectioners' sugar, sifted
To make the cake
Preheat oven to 350
In a medium bowl, whisk together flour, baking powder, baking soda and salt.
In a large mixing bowl, cream together butter and sugar until light and fluffy. Add the eggs and egg whites, one at a time, mixing until completely combined. Mix in the lemon zest, lime zest and lime juice.
Add flour mixture and buttermilk alternately to sugar mixture, beginning and ending with flour mixture. Scoop the mixture into two 9" baking pans coated with nonstick spray.
Bake until a toothpick placed in center comes out mostly clean with a few moist crumbs attached, about 25 to 30 minutes. Remove and place on a wire rack to cool for 10 minutes - remove cakes from pans and let cool completely.
To make the icing
In a large mixing bowl, beat together cream cheese, lemon zest, lime zest and juice until smooth. Gradually add confectioners' sugar - beat at low speed just until combined, do not overbeat. Cover and place in the refrigerator to chill for 30 minutes.
To assemble, place 1 cake layer on a plate - cover the top with about 1/2 cup icing. Top with remaining cake layer. Spread remaining icing over top - place cake in the refrigerator to chill for about 1 hour to set the icing.
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HP LaserJet Pro M127fn Laser Printer
Product Code: CZ181A
Buy HP LaserJet Pro M127fn Laser Printer Cartridges online today and save! Cartridges Direct offers FREE next-day delivery on all orders over $75
Hewlett Packard LaserJet Pro M127fn Laser Printer
Printer Model
Print Copy Scan Fax
One-Year limited warranty
Duty Cycle
Up to 8000 Pages
Max. Paper Size
Hi-Speed USB 2.0 port built-in Fast Ethernet 10/100Base-TX network port
Pages Per Minute
20 - 34ppm
HP LaserJet Pro M127fn Laser Printer
- This affordable, full-featured HP LaserJet Pro MFP is easy to install—and prints, copies, scans, and faxes. Enable easy printing from smartphones, tablets, and laptops—at work, home, or on the go—using mobile printing features.1
Handle the essentials
Use the simple, intuitive control panel to easily set up projects and start printing right away.
Finish projects fast—use the 35-page automatic document feeder to make the most of your time.
Simplify copying—quickly capture two-sided documents in fewer steps.
Set the pace for success with an MFP that prints, copies, and scans—fast.
Print on your terms
Now you're wired. Share all your printer has to offer, company-wide, using 10/100 Ethernet connectivity.
Print from your mobile device with built-in print capability or HP ePrint app and software.1
Print from virtually anywhere with AirPrint™.2
- Functions:
- Print, copy, scan, fax
- Multitasking supported:
- Yes
Printing specifications
- Print speed black:
- Normal: Up to 20 ppm
(Measured using ISO/IEC 24734, excludes first set of test documents. For more information see hp.com/products1/ISO/MMDPP/index.html. Exact speed varies depending on the system configuration, software application, driver, and document complexity.)
- First page out (ready)
- Black: As fast as 9.5 sec
- Duty cycle (monthly, A4)
- Up to 8000 pages
(Duty cycle is defined as the maximum number of pages per month of imaged output. This value provides a comparison of product robustness in relation to other HP LaserJet or HP Color LaserJet devices, and enables appropriate deployment of printers and MFPs to satisfy the demands of connected individuals or groups.)
- Recommended monthly page volume
- 250 to 2000
(HP recommends that the number of pages per month of imaged output be within the stated range for optimum device performance, based on factors including supplies replacement intervals and device life over an extended warranty period.)
- Print technology:
- Laser
- Print quality black (best):
- Up to 600 x 600 dpi
- Print languages:
- PCLm/PCLmS
- Display:
- 2-line LCD (text)
- Processor speed:
- 600 MHz
- Automatic paper sensor:
- No
- Replacement cartridges
- HP 83A Black LaserJet Toner Cartridge (~1500 yield) CF283A
(Replacement cartridge average declared yield ~1500 pages based on ISO/IEC 19752 and continuous print. Actual yields vary considerably based on images printed and other factors. For more information, visit: hp.com/go/learnaboutsupplies)
(Introductory cartridge included; ~700 pages based on ISO/IEC 19752 and continuous print. Replacement cartridge average declared yield ~1500 pages based on ISO/IEC 19752 and continuous print. Actual yields vary considerably based on images printed and other factors. For more information, visit: hp.com/go/learnaboutsupplies)
- HP ePrint capability:
- Yes
- Mobile printing capability
- HP ePrint, Apple AirPrint™, Mobile Apps
(May require a firmware upgrade to be compatible, download at hp.com/go/support)
- Wireless capability:
- No
- Connectivity, standard:
- Hi-Speed USB 2.0 port
- built-in Fast Ethernet 10/100Base-TX network port
- Network ready:
- Standard (built-in Ethernet)
- Minimum system requirements
- Windows 10 (32-bit/64-bit), Windows 8 (32-bit/64-bit), Windows 7 (32-bit/64-bit), Windows Vista (32-bit/64-bit), Windows XP (32-bit) (SP2 or higher)
- 200 MB hard disk space required (CD and Web Package)
- 400 MB hard disk space required
- 512 MB RAM installed
- CD-ROM/DVD or Internet
- USB or Network port
- Mac OS X v 10.6
- PowerPC G4, G5, or Intel® Core™ Processor
- 500 MB available hard disk space
- CD-ROM/DVD-ROM or Internet
- USB or Network port
- Compatible operating systems
- Full software installs supported on: Windows 10 (32-bit/64-bit), Windows 8 (32-bit/64-bit), Windows 7 (32-bit/64-bit), Windows Vista (32-bit/64-bit), Windows XP (32-bit) (SP2 or higher)
Driver only installs supported on: Windows Server 2012
Windows Server 2008 (32-bit), Windows Server 2008 (Standard Edition), Windows Server 2008 (Enterprise Edition), Windows Server 2003 (32-bit) (SP1 or higher)
Mac OS X v 10.6.8 or higher
Linux: Please check Linux on-line support: http://www.hplipopensource.com/hplip-web/install.html
(Driver only installs supported on: Windows Server 2012; Windows Server 2008 (32-bit), Windows Server 2008 (Standard Edition), Windows Server 2008 (Enterprise Edition), Windows Server 2003 (32-bit) (SP1 or higher))
Memory specifications
- Memory, standard:
- Maximum memory
Paper handling
- Paper handling input, standard:
- 150-sheet input tray
- Paper handling output, standard:
- 100-sheet face-down bin
- Maximum output capacity (sheets):
- Up to 100 sheets
- Duplex printing:
- Manual (driver support provided)
- Media sizes supported:
- B5-Japanese
- envelopes (ISO DL, C5, B5, Com #10, Monarch #7 3/4)
- post cards (Standard #10, JIS single and double)
- Media sizes, custom:
- 76 x 127 to 216 x 356 mm
- Media types:
- Paper (plain, LaserJet), envelopes, transparencies, labels, postcards
- Media weight, supported:
- 60 to 163 g/m²
- Media weights, supported ADF:
- 60 to 90 g/m²
Scanner specifications
- Scanner type:
- Flatbed, ADF
- Scan file format:
- Windows Scan SW supports file format: JPG, RAW(BMP), PDF, TIFF, PNG
- Mac Scan SW supports file format: TIFF, PNG, JPEG, JPEG-2000, PDF, PDF-Searchable, RTF, TXT
- Scan resolution, optical:
- Up to 1200 dpi
- Scan size (flatbed), maximum:
- 216 x 297 mm
- Scan size (ADF), maximum:
- 216 x 356 mm
- Scan size ADF (minimum):
- 152 x 114 mm
- Scan speed (normal, A4)
- Up to 7 ppm (b&w), up to 5 ppm (color)
- Duplex ADF scanning:
- No
- Automatic document feeder capacity:
- Standard, 35 sheets
- Digital sending standard features:
- Scan to E-mail by HP SW
- scan to application by HP SW
- scan to file by HP SW
- File formats, supported:
- Scan input modes:
- Scanning via HP LaserJet scan application or TWAIN—or WIA-compliant application software
Copier specifications
- Copy speed (normal)
- Black: Up to 20 cpm
(First Copy Out and Copy Speed measured using ISO/IEC 29183, excludes first set of test documents. For more information see hp.com/products1/ISO/digital_copy/index.html. Exact speed varies depending on the system configuration, software application, driver, and document complexity.)
- Copy resolution (black text):
- Up to 600 x 600 dpi
- Copy reduce / enlarge settings:
- 25 to 400%
- Copies, maximum:
- Up to 99 copies
Fax specifications
- Faxing:
- Yes
- Fax transmission speed
- 33.6 kbps
(Based on standard ITU-T test image #1 at standard resolution. More complicated pages or higher resolution will take longer and use more memory.)
- Fax memory:
- Up to 500 pages
- Fax resolution:
- Up to 300 x 300 dpi
- Speed dials, maximum number:
- Up to 100 numbers
- Broadcast locations:
- 100 locations
Power and operating requirements
- Power:
- Input voltage: 110 to 127 VAC (+/- 12%), 60 Hz (+/- 3 Hz)
- Input voltage: 220 to 240 VAC (+/- 12%), 50/60 Hz (+/- 3 Hz) (Not dual voltage, power supply varies by part number with # Option code identifier)
- Power consumption
- 480 watts (Printing), 186 watts (Copying from ADF), 4.5 watts (Ready), 1.1 watts (Sleep/Auto-Off), 0.2 watts (Manual Off)
(Values subject to change. Power numbers are the highest values measured using all standard voltages. See hp.com/support for current information. Power requirements are based on the country/region where the printer is sold. Do not convert operating voltages. This will damage the printer and void the product warranty.)
- Energy efficiency:
- ENERGY STAR® qualified
- Operating temperature range:
- 15 to 32.5ºC
- Operating humidity range:
- 30 to 70% RH
- Acoustic power emissions (ready)
- Inaudible
(Acoustic values are subject to change. For current information see hp.com/support. Configuration tested: LaserJet Pro MFP M127fw, simplex printing with A4 paper. LaserJet Pro MFP M127fw, ADF copy and print simplex using A4 paper.)
- Acoustic pressure emissions bystander (active, printing)
- 49 dB(A)
(Acoustic values are subject to change. For current information see hp.com/support. Configuration tested: LaserJet Pro MFP M127fw, simplex printing with A4 paper. LaserJet Pro MFP M127fw, ADF copy and print simplex using A4 paper.)
Dimensions and weight
- Minimum dimensions (W x D x H)
- 420 x 365 x 309 mm
- Maximum dimensions (W x D x H)
- 420 x 438 x 309 mm
- Weight
- 9.1 kg
- Package weight:
- 11.5 kg
What's included
- What's in the box
- HP LaserJet Pro MFP M127fn
Preinstalled introductory HP LaserJet Black Cartridge (~700 pages)
Installation guide, Getting Started guide, User's Guide, Support flyer, Warranty guide
Printer software and documentation on CD
power cord
USB cable
Phone cord
- Cable included:
- Yes, 1 USB
- Software included:
- Windows: HP Installer/Uninstaller, HP PCLmS print driver, HP WIA Scan Driver, HP TWAIN Scan Driver, HP Scan, HP Fax Setup Wizard, HP Send Fax, Status Alerts, HP Update, DXP, Readiris Software
- Mac: HP Installer/Uninstaller, HP Postscript driver, HP Scan, HP Setup Assistant, HP Fax Print Driver, HP Utility, HP Alerts, HP Firmware Updater, Readiris Software
- Warranty:
- One-year limited warranty
Need Help with your purchase? | |
If you have any questions regarding any of our products or services, please feel free to contact us. | |
1300 765 575 | |
03 9703 255 | |
firstname.lastname@example.com | |
Find out more | |
Delivery and Shipping Information | |
Terms and Conditions |
Be the first to write a review


text:
The Oscars are finally upon us, after months of anticipation and excitement for the film industry and its fans alike. While I do enjoy speculating on the winners and losers (those lucky, lucky losers) prior to the awards, I am much more inclined to just sit back, eat some snacks and enjoy the show. I like to take bets on who's going to cry, who's going to be caught on camera giving dirty looks and whose jokes are going to flop entirely while munching on some great treats. Of course, those snacks need to be properly thought out according to those who are up for awards.
Yesterday, Carrie brought you some delightfully healthful and gorgeous foods beloved by the women who are nominated for Best Actress at the Oscars tonight. If you're an awards show fiend and are looking for more food for the Oscars, take a glance at this guide full of recipes inspired by the Best Picture nominees. There were so many incredible films this year and the ones nominated for the ultimate award are pretty difficult to choose from prediction-wise*, so why not just choose a bunch of them? Well, choose their recipes, at least. Even if most of them will lose the award in the end, all of these foods and drinks are winners, so try 'em all!
*I am totally rooting for Quvenzhané Wallis all the way, though.


text:
- Video
- Overview
- Information
- Reviews
- Help
Rock Climbing BRD Belay Rappel Device Element Carabiner
Shopping Info
Metolius Rock Climbing BRD Belay Device With Element Carabiner - The BRD Belay Device With Element Carabiner by Metolius offers enhanced control while lowering and rappelling with enormous stopping power. This versatile and lightweight belay/rappel device has rope slots are that are engineered to increase friction on the rope allowing the belayer to easily catch long, hard falls with relatively light hand pressure. The principle behind the BRD is very simple: as the load of a falling climber is applied, the carabiner is pulled into the scientifically engineered rope slots and the rope is squeezed between the carabiner and the BRD. CNC machined from 6061 T-6 aluminum and hard anodized for longer wear the cooling fins help to dissipate heat more efficiently. So if you are in the market for a versatile and lightweight device, look no further than the BRD Belay Device With Element Carabiner by Metolius.
Metolius BRD Belay Device With Element Carabiner - Single or double rope compatible, 8 mm to 11 mm ropes. |
I have yet to rappel with this device but on belay duty it does just fine. I'm a little skeptical as to the actual effectiveness of the super awesome "cooling fins". Either way, it performs properly and makes speedy catches and it's pretty thrifty deal as well.
Bend, OR
BRD Belay Device with Element Carabiner by Metolius for Rock Climbing
Need More Information About This Item?
Contact Us by Phone
Speak with an expert at 800.994.4327 Mon-Fri 7am-5pm PST.


text:
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Maca root has been used in indigenous Andean societies as a source of nourishment and healing for thousands of years. This bag contains 100% pure raw maca powder that is certified organic, kosher, vegan and raw. Studies have identified four alkaloids present in maca known to nourish the endocrine system. Maca root has been used as a stress-fighting adaptogen to increase stamina, boost libido and combat fatigue. Maca is a nutrient-dense whole food packed with vitamins, plant sterols as well as many essential minerals, fatty and amino acids. Our raw maca is low temperature processed and cultivated on the Junin Plateau of Peruvian Andes. Navitas Naturals? mission is to provide premium organic power foods that increase energy and enhance health. Our products are of the highest quality, sourced directly from farmers, cultivated sustainably and in accordance with fair trade practices. Product of Peru - Packed in USA
Features include:
•Certified Organic, Kosher, Vegan and Raw.
•Made from pure, 100% organic Maca roots.
•Amino Acids, Essential Mineals. Adaptogen.
•Mix into smoothies, baked goods, or cereal; may boost the libido, treat menopause symptoms, and promote hormonal balance for men and women
•Navitas Naturals products come directly from the farmers who cultivate them and are free of heavy metals, chemicals and pesticides.
Product Notes
Description | Unit Price |
Organic, Raw Maca Powder, 16oz |
Discontinued by vendor |
Please choose which cart you want to add the item.
By choosing one of the above, you are able to shop for both yourself and a registrant at the same time. Once you are ready to checkout your items will be waiting for you in both your personal and registry shopping carts.
Still need assistance?
Please contact us at SOAP123 (1-800-762-7123)
Reviewed by 1 customer
Displaying review 1
Comments about Organic, Raw Maca Powder, 16oz:
It may seem pricey at first blush, but you only need a little at a time, so it is a good value in the end. Definitely a nice addition to my morning juice or smoothies.
Displaying review 1


text:
Badass truck! Glws man
--------------WILL BE AT OTM COME BY AND TAKE A LOOK----------------------- Text/call 713-299-5827 JR
I copy/pasted from the last for sale thread whit some revisions. Lowered price and added more stuff to the truck.
Selling my 2006 Intimidator SS #768 of 933, some specs:
Engine/trans/rear end:
LQ9 6.0 w/ 60,1XX miles (will go up alittle depending on when/if it sells)
8.1L marine injectors (#42)
Volant C.A.I (Powercore dry filter)
LPP Longtube stainless steel headers
Corsa Touring catback w/ Magnaflow hi-flow cats
4L65E w/trans go shift kit, corvette servo and acc. piston
Circle D 3200 278MM Converter
40K trans cooler
14Bolt w/3.73's and G80
Tuned by Jake
22X8.5 Bonspeed Intense 6's
275/45/22 Cooper tires
Energy suspension sway bar end links and bushings
Mcgaughys 2" drop spindels, purple keys and belltech street performance shocks
Mcgaughys drop hangers/shackles, Belltech street performance shocks and Mcgaughys Helper bags, 1 has a hole from when i put on a spare and the tire rubbed it
C6 ZO6 6 piston calipers up front, 15" baer rotors.
C6 ZO6 4 piston calipers out back, 14" baer rotors.
Russell SS brake lines.
Parking/emergency brake lines are not currently connected, i do have them tho.
Just painted trim piece on dash bezel
TBSS airbag whit custom "SS" badge
Panasonic double din
12" P3 sub and amp (Not sure the model number)
Im missing some stuff but that about covers most of it.
Now on to the bad stuff:
I did drive it alot so it has some little rock chips/light scratches, has a dent on the edge of the hood and little dents on the bed rails, Drivers seat shows alittle wear and the bed, well it's scrached up from putting stuff back there. I disconected the heated seat feature, it would come on randomly IDK why many other SS owners have had the same problem. I used to have a flip down screen and removed it, has 4 little holes now a suede headliner will take care of that tho. Little exhaust leak on the Y-pipe by the crossmember.
Never had any mechanical problems, i change the oil every 3-5K whit Mobile 1 and 206 mibile 1 filter. All maintenance items have been done, tune-up, trans fluids, rear end fluid ect.
Price as-is: $27,000
Clean Texas Title in hand.
Trades: im looking for a regular cab 99-06 if it has a 6.0 that would be nice but stock is fine. I dont want someones mistake tho, if you know what i mean, of course +cash on your side.
Some pics.
Side shot to show the brakes
Only interior shot, everything else is pretty much stock. I have the original "Intimidator SS" floor mats, took them out when i first bought the truck in 06' and they have been stored since.
Thanks for looking.
Last edited by NTMD8R_SS; 10-26-2011 at 02:21 PM.
06 ISSHidden Content -FOR SALE...........Billets whit a little lip but BIG brakes!
Sick truck Glws/t
Hidden Content
Im allergic...... Aaachoooo! to hatters.
Forgot once again its #768 of 933 made and the air bag matches, i took the pic when it was bearly put on before the armor all lol.
06 ISSHidden Content -FOR SALE...........Billets whit a little lip but BIG brakes!
This is one of the cleanest trucks on here glws bro
Sick SS if I had the cash I'll buy it
Nice truck bro, glws
Hidden Content
LT headers|Roadkill V3R cam|arp Dual springs|3800/4k circle D|CAI|magnaflow|servo|shift kit
Bump for badass truck. Glws!!
nice ss glws
2007 Gmc Sierra 4.8 with 4.56 gears Magnaflow 3' CAI tuned by KHP
nice ss jr,,,glws
bad ass ss glws
Just your average 5.3
nice truck but price out of my league
Thanks guys. Just looked it up on KBB and for a regular 06SS its about 23k I could maybe get near that price it will have to be taken down to stock tho, but for 4K more i think mine is worth it and price is O.B.O too. Like i said i am taking trades so that will make it more affordable for someone, i know i will never get what i have in it (Over 47K including the purchase price)
06 ISSHidden Content -FOR SALE...........Billets whit a little lip but BIG brakes!


text: Petey Throws Five Innings Of Slop For The WinA.J. Daulerio8/13/09 9:45amFiled to: MlbPedro MartinezPhiladelphia PhilliesWorld fucking champions18EditPromoteShare to KinjaToggle Conversation toolsGo to permalinkIt wasn't pretty, or inspiring, but he did end up with the win. And Charlie Manuel used the hillbilly logic to monitor his new, old pitcher: "Five innings and he was ready to be got." [Philly.com]Reply18 repliesLeave a reply


text:
Taylor on the road to fitness
West Ham, Updated:
[.[views].], BY ClubCall
West Ham have been handed a boost on the injury front as Matthew Taylor looks to have recovered from being knocked out last weekend.
With no match until a week on Sunday, when the Hammers travel to Chelsea, it is hoped that Taylor will not miss any action but he was still left shaken up by the incident.
He was caught in the face by Peter Crouch's flying boot as he attempted an overhead kick and it knocked him out cold and he had to be helped from the field.
"I am not too bad. My head is fine and I am feeling a lot better," Taylor told the club's website,
"It is the first time I have been knocked out in my career, or in my life for that matter, that I can recall. If it was going to happen anywhere, then on a football pitch is not a bad place because of all the medical staff being there.
"I don't have any recollection of heading the ball. My only memory I have is the ball going over my head [from the corner] and following my runner.
"I then remember waking up in their treatment room on the treatment table. I don't have any other memory. I don't remember walking off or anything, which was a bit scary."


text:
From eLinux.org
Eoma200pcblayout.txt (file size: 3 KB, MIME type: application/x-env)
Warning: This file type may contain malicious code.
By executing it, your system may be compromised.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Dimensions | User | Comment | |
current | 16:00, 9 April 2013 | (3 KB) | Lkcl (Talk | contribs) |
- You cannot overwrite this file.
File usage
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text:
Related Sections: | Jamul | San Diego County |
Steele Canyon Golf & Country Club - Canyon/Ranch Course
3199 Stonefield DrJamul, CA 91935
Phone: (619)441-6900Fax: (619)441-6909
This club has three nine hole courses that are played in three eighteen hole combinations. The three courses are very different and challenging. The Ranch/Meadow Course is the most challenging due to the hilly terrain. The width of each fairway varies from narrow to wide open, and the fast greens are medium-sized. There are out-of-bounds stakes on all three courses, and several old oak trees come into play along the fairways. These very scenic courses are highlighted by a beautiful lake.
Holes: 18 | Greens: Bent Grass | Fairways: Bermuda Grass |
Greens Fees: | $50-$79 |
Course Type: | semi-private |
Dress Code: | No denim, collared shirt and bermuda shorts required |
Season: | Open all year |
Guest Policy: |
* Update this Course: Update/modify the details for this golf course's listing.
*All Greens Fees provided are subject to change. Please call the golf course to confirm the current fees.


text:
Dad, grandma are donors for child's double transplant
Family shows twice the love
September 21, 2007 8:00 AMSince his birth, Andrew Morrissey has held a central place in the affections of the adults who surround him. And now the bonds of a family are drawn even closer: The 17-month-old from Maryland has received a combination liver/kidney transplant, with organs donated by his father and a grandmother.
Speaking yesterday afternoon, Dr. George V. Mazariegos, the child's surgeon, said Andrew was recovering well.
The three patients, all from suburban Baltimore, continued to be closely watched in intensive care yesterday after 14 hours of surgery Wednesday at both Children's and Montefiore hospitals.
Andrew, born March 27, 2006, suffered from a congenital kidney and liver disorder known as autosomal recessive polycystic kidney disease. He had both kidneys removed by the time he was a month old. He had developed severe complications from inflammation of the bile duct.
On dialysis since his second kidney removal, Andrew also had a diseased liver.
For much of his young life, he has been in and out of the hospital, mostly because of liver infections. His nephrology team at the University of Maryland Medical Center referred his case to Children's.
"There was a question of whether one or two transplants would be needed," said Dr. Mazariegos, director of pediatric transplantation at Children's Hospital's Hillman Center for Pediatric Transplantation. "He clearly needed the kidney. The risk of ongoing dialysis is very high."
If he had only a kidney transplant and was put on immunosuppressant medication, the doctor said, his risk of infection in the bile duct would rise.
The transplant cures Andrew of the disease he was born with, Dr. Mazariegos said.
Michael Morrissey, 29, a mechanical and environmental engineer, gave one-quarter of his liver to his son, said Dr. Mazariegos. Dr. Rakesh Sindhi, co-director of pediatric transplantation, and Dr. Amadeo Marcos removed the liver at Montefiore and carried it to Children's for the transplant, performed by Dr. Mazariegos and another transplant surgeon, Dr. Kyle Soltys.
Dee Papania, 53, who works for the archdiocese of Baltimore as a pastoral associate, gave one of her kidneys to her first grandchild.
The operation was only the second of that type done by the transplant team at Children's, Dr. Mazariegos said. The first patient, a 6-year-old boy received a kidney and liver from living donors in April and is reportedly doing well.
Living donors -- almost always family members -- give the patient the advantage of avoiding a long waiting list for deceased-donor organs and receiving organs that more closely match their own.
Dr. Mazariegos said that the team has worked with 10 live donors this year, about one-third of its total transplant cases.
"The results of adult-to-children [transplant] have been exceptional," he said.
He said Andrew will likely be on some immunosuppressant drugs for life, although "we try to wean it to the lowest level possible. We have noted that in living-donor transplants, the need for immunosuppressants is minimal. Up to 10 percent of the patients can have it withdrawn. That is an encouraging possibility. We're working on predicting which patients fall into that category."
He predicted that Andrew will spend at least a week in intensive care, the next stage of a journey that began 17 months earlier.
"Supposedly, Andrew should have died in 48 hours after his birth," said John Papania, his maternal grandfather. "He's a miracle. We believe God's got a plan for him."
The Papania and Morrissey families were first evaluated for transplantation in June. They found out they had matches on July 18. On Aug. 2, Andrew's father and maternal grandmother, Dee, were evaluated to make sure they were healthy enough to donate.
Andrew's mother, Jess, said her husband was chosen for the liver because he was more fit and could better stand the arduous liver surgery. Dee's kidneys were smaller and deemed more suitable for the baby.
"We were always very nervous," Jess Morrissey said, "But [Tuesday] it started to get a little scary. We had our day-to-day routine. Now the unknown is a little scary."
Tuesday night, Andrew had a high fever, she said, but after a medication was changed, he was back to his cheerful, playful self and stayed up till 3:45 a.m. Wednesday -- hours before surgery was to begin.
Family members expect to remain in Pittsburgh for four to five months.
The unknown affected the whole family, Mr. Papania said.
"But we're seriously people of faith. I don't see how other people manage without faith. The stress does zap you. But God has a plan."
For the Morrissey family's updates on Andrew's story, see www.caringbridge.org/visit/andrewmorrissey. For more on ARPKD, see the Web site of the ARPKD/CHF Alliance: www.arpkdchf.org/.
Jill Daly can be reached at email@example.com or 412-263-1596. First Published September 21, 2007 4:00 AM


text:
3700 N Wilke Rd
Arlington Heights, IL 60004
Courtyard Arlington Hts, lets you stay in style whenever or wherever you travel. With indoor and outdoor spaces, comfortable rooms and suites, and wifi in every hotel, make Courtyard your next stay.
In ShortThe first Marriott hotel opened in 1957, and has since expanded to several different brands, with Courtyard serving business travelers. In addition to free daily newspaper...
If you've been to or used Courtyard by Marriott Chicago Arlington Heights/North, leave a review.
It's easy, only takes a couple of minutes and you'll help thousands make an informed decision.


text:
DNA helicase | |||||||||
Identifiers | |||||||||
EC number | 22.214.171.124 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
RNA helicase | |||||||||
Identifiers | |||||||||
EC number | 220.127.116.11 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Helicases are a class of enzymes vital to all living organisms. Their main function is to unpackage an organism's genes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e., DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. There are many helicases resulting from the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases.[1] The human genome codes for 95 non-redundant helicases: 64 RNA helicases and 31 DNA helicases.[2] Many cellular processes, such as DNA replication, transcription, translation, recombination, DNA repair, and ribosome biogenesis involve the separation of nucleic acid strands that necessitates the use of helicases.
Helicases are often used to separate strands of a DNA double helix or a self-annealed RNA molecule using the energy from ATP hydrolysis, a process characterized by the breaking of hydrogen bonds between annealed nucleotide bases. They also function to remove nucleic acid-associated proteins and catalyze homologous DNA recombination.[3] Metabolic processes of RNA such as translation, transcription, ribosome biogenesis, RNA splicing, RNA transport, RNA editing, and RNA degradation are all facilitated by helicases.[3] Helicases move incrementally along one nucleic acid strand of the duplex with a directionality and processivity specific to each particular enzyme.
Helicases adopt different structures and oligomerization states. Whereas DnaB-like helicases unwind DNA as ring-shaped hexamers, other enzymes have been shown to be active as monomers or dimers. Studies have shown that helicases may act passively, waiting for uncatalyzed unwinding to take place and then translocating between displaced strands,[4] or can play an active role in catalyzing strand separation using the energy generated in ATP hydrolysis.[5] In the latter case, the helicase acts comparably to an active motor, unwinding and translocating along its substrate as a direct result of its ATPase activity.[6] Helicases may process much faster in vivo than in vitro due to the presence of accessory proteins that aid in the destabilization of the fork junction.[6]
Activation barrier in helicase activity[edit]
Enzymatic helicase action, such as unwinding nucleic acids is achieved through the lowering of the activation barrier (B) of each specific action.[7] The activation barrier is a result of various factors, and can be defined using the following equation, where N = number of unwound base pairs (bps), ΔGbp = free energy of base pair formation, Gint = reduction of free energy due to helicase, and Gƒ = reduction of free energy due to unzipping forces.[7]
- B = N (ΔGbp-Gint-Gƒ)
Factors that contribute to the height of the activation barrier include: specific nucleic acid sequence of the molecule involved, the number of base pairs involved, tension present on the replication fork, and destabilization forces.[7]
Active and passive helicases[edit]
The size of the activation barrier to overcome by the helicase contributes to its classification as an active or passive helicase. In passive helicases, a significant activation barrier exists (defined as B > kBT, where kB is Boltzmann's constant and T is temperature of the system).[7] Because of this significant activation barrier, its unwinding progression is affected largely by the sequence of nucleic acids within the molecule to unwind, and the presence of destabilization forces acting on the replication fork.[7] Certain nucleic acid combinations will decrease unwinding rates (i.e. guanine and cytosine), while various destabilizing forces can increase the unwinding rate.[7] In passive systems, the rate of unwinding (Vun) is less than the rate of translocation (Vtrans) (translocation along the single-strand nucleic acid, ssNA).[7] Another way to view the passive helicase is its reliance on the transient unraveling of the base pairs at the replication fork to determine its rate of unwinding.[7]
In active helicases, B < kBT, where the system lacks a significant barrier, as the helicase is able to destabilize the nucleic acids, unwinding the double-helix at a constant rate, regardless of the nucleic acid sequence.[7] In active helicases, Vun is approximately equal to Vtrans.[7] Another way to view the active helicase is its ability to directly destabilize the replication fork to promote unwinding.[7]
Active helicases show similar behavior when acting on both double-strand nucleic acids, dsNA, or ssNA, in regards to the rates of unwinding and rates of translocation, where in both systems Vun and Vtrans are approximately equal.
These two categories of helicases may also be modelled as mechanisms. In such models the passive helicases are conceptualized as Brownian ratchets, driven by thermal fluctuations and subsequent anisotropic gradients across the DNA lattice. The active helicases, in contrast, are conceptualized as stepping motors - also known as powerstroke motors - utilizing either a conformational "inch worm" or a hand-over-hand "walking" mechanism to progress.[8] Depending upon the organism, such helix-traversing progress can occur at rotational speeds in the range of 5,000 [9] to 10,000 [10] R.P.M.
History of DNA helicases[edit]
DNA helicases were discovered in E. coli in 1976. This helicase was described as a "DNA unwinding enzyme" that is "found to denature DNA duplexes in an ATP-dependent reaction, without detectably degrading".[11] The first eukaryotic DNA helicase was in 1978 in the lily plant.[12] Since then, DNA helicases were discovered and isolated in other bacteria, viruses, yeast, flies, and higher eukaryotes.[13] To date, at least 14 different helicases have been isolated from single celled organisms, 6 helicases from bacteriophages, 12 from viruses, 15 from yeast, 8 from plants, 11 from calf thymus, and approximately 25 helicases from human cells.[14] Below is a history of helicase discovery:
- 1976 – Discovery and isolation of E. coli-based DNA helicase[11]
- 1978 – Discovery of the first eukaryotic DNA helicases, isolated from the lily plant[12]
- 1982 – "T4 gene 41 protein" is the first reported bacteriophage DNA helicase[13]
- 1985 – First mammalian DNA helicases isolated from calf thymus[15]
- 1986 – SV40 large tumor antigen reported as a viral helicase (1st reported viral protein that was determined to serve as a DNA helicase)[16]
- 1986 – ATPaseIII, a yeast protein, determined to be a DNA helicase[17]
- 1988 – Discovery of seven conserved amino acid domains determined to be helicase motifs
- 1989 – Designation of DNA helicase Superfamily I and Superfamily II[18]
- 1989 - Identification of the DEAD box helicase family[19]
- 1990 - Isolation of a human DNA helicase[20]
- 1992 – Isolation of the first reported mitochondrial DNA helicase (from bovine brain)[21]
- 1996 – Report of the discovery of the first purified chloroplast DNA helicase from the pea[22]
- 2002 – Isolation and characterization of the first biochemically active malarial parasite DNA helicase - Plasmodium cynomolgi.[23]
Structural features[edit]
The common function of helicases accounts for the fact that they display a certain degree of amino acid sequence homology; they all possess sequence motifs located in the interior of their primary structure, involved in ATP binding, ATP hydrolysis and translocation along the nucleic acid substrate. The variable portion of the amino acid sequence is related to the specific features of each helicase.
The presence of these helicase motifs allows putative helicase activity to be attributed to a given protein, but does not necessarily confirm it as an active helicase. Conserved motifs do, however, support an evolutionary homology among enzymes. Based on these helicase motifs, helicases a number of superfamilies have been distinguished.
Helicases are classified in 6 groups (superfamilies) based on their shared sequence motifs.[24] Helicases not forming a ring structure are in superfamilies 1 and 2, and ring-forming helicases form part of superfamilies 3 to 6.[25] Helicases are also classified as α or β depending on if they work with single or double-strand DNA; α helicases work with single-strand DNA and β helicases work with double-strand DNA. They are also classified by translocation polarity. If translocation occurs 3'-5' the helicase is type A; if translocation occurs 5'-3' it is type B.[24]
- Superfamily 1 (SF1): This superfamily can be further subdivided into SF1A and SF1B helicases.[24] In this group helicases can have either 3'-5' (SF1A subfamily) or 5'-3'(SF1B subfamily) translocation polarity.[24][26] The most known SF1A helicases are Rep and UvrD in gram-negative bacteria and PcrA helicase from gram-positive bacteria.[24] The most known Helicases in the SF1B group are RecD and Dda helicases.[24]
- Superfamily 2 (SF2): This is the largest group of helicases that are involved in varied cellular processes.[24][27] They are characterized by the presence of nine conserved motifs: Q, I, Ia, Ib, and II through VI.[27] This group is mainly composed of DEAD-box RNA helicases.[25] Some other helicases included in SF2 are the RecQ-like family and the Snf2-like enzymes.[24] Most of the SF2 helicases are type A with a few exceptions such as the XPD family.[24]
- Superfamily 3 (SF3): Superfamily 3 consists of helicases encoded mainly by small DNA viruses and some large nucleocytoplasmic DNA viruses.[28][29] They have a 3'-5' translocation directionality, meaning that they are all type A helicases.[24] The most known SF3 helicase is the papilloma virus E1 helicase.[24]
- Superfamily 4 (SF4): All SF4 family helicases have a type B polarity (5'-3').[24] The most studied SF4 helicase is gp4 from bacteriophage T7.[24]
- Superfamily 5 (SF5): Rho proteins conform the SF5 group.[24]
- Superfamily 6 (SF6): They contain the core AAA+ that is not included in the SF3 classification.[24] Some proteins in the SF6 group are: mini chromosome maintenance MCM, RuvB, RuvA, and RuvC.[24]
Helicase disorders and diseases[edit]
ATRX helicase mutations[edit]
The ATRX gene encodes the ATP-dependent helicase, ATRX (also known as XH2 and XNP) of the SNF2 subgroup family, that is thought to be responsible for functions such as chromatin remodeling, gene regulation, and DNA methylation.[30][31][32][33] These functions assist in prevention of apoptosis, resulting in cortical size regulation, as well as a contribution to the survival of hippocampal and cortical structures, affecting memory and learning.[30] This helicase is located on the X chromosome (Xq13.1-q21.1), in the pericentromeric heterochromatin and binds to heterochromatin protein 1.[30][32] Studies have shown that ATRX plays a role in rDNA methylation and is essential for embyonic development.[34] Mutations have been found throughout the ATRX protein, with over 90% of them being located in the zinc finger and helicase domains.[35] Mutations of ATRX can result in X-linked-alpha-thalassaemia-mental retardation (ATR-X syndrome).[30]
Various types of mutations found in ATRX have been found to be associated with ATR-X, including most commonly single-base missense mutations, as well as nonsense, frameshift, and deletion mutations.[33] Characteristics of ATR-X include: microcephaly, skeletal and facial abnormalities, mental retardation, genital abnormalities, seizures, limited language use and ability, and alpha-thalassemia.[30][34][36] The phenotype seen in ATR-X suggests that the mutation of ATRX gene causes the downregulation of gene expression, such as the alpha-globin genes.[36] It is still unknown what causes the expression of the various characteristics of ATR-X in different patients.[34]
XPD helicase point mutations[edit]
XPD (Xeroderma pigmentosum factor D, also known as protein ERCC2) is a 5'-3', Superfamily II, ATP-dependent helicase containing iron-sulphur cluster domains.[37][38] Inherited point mutations in XPD helicase have been shown to be associated with accelerated aging disorders such as Cockayne syndrome (CS) and trichothiodystrophy (TTD).[39] Cockayne syndrome and trichothiodystrophy are both developmental disorders involving sensitivity to UV light and premature aging, and Cockayne syndrome exhibits severe mental retardation from the time of birth.[39] The XPD helicase mutation has also been implicated in xeroderma pigmentosa (XP), a disorder characterized by sensitivity to UV light and resulting in a several 1000-fold increase in the development of skin cancer.[39]
XPD is an essential component of the TFIIH complex, a transcription and repair factor in the cell.[39][40][41][42][43] As part of this complex, it facilitates nucleotide excision repair by unwinding DNA.[39] TFIIH assists in repairing damaged DNA such as sun damage.[39][40][41][42][43] A mutation in the XPD helicase that helps form this complex and contributes to its function causes the sensitivity to sunlight seen in all three diseases, as well as the increased risk of cancer seen in XP and premature aging seen in trichothiodystrophy and Cockayne syndrome.[39]
XPD helicase mutations leading to trichothiodystrophy are found throughout the protein in various locations involved in protein-protein interactions.[39] This mutation results in an unstable protein due to its inability to form stabilizing interactions with other proteins at the points of mutations.[39] This, in turn, destabilizes the entire TFIIH complex, which leads to defects with transcription and repair mechanisms of the cell.[39]
It has been suggested that XPD helicase mutations leading to Cockayne syndrome could be the result of mutations within XPD, causing rigidity of the protein and subsequent inability to switch from repair functions to transcription functions due to a "locking" in repair mode.[39] This could cause the helicase to cut DNA segments meant for transcription.[39] Although current evidence points to a defect in the XPD helicase resulting in a loss of flexibility in the protein in cases of Cockayne syndrome, it is still unclear how this protein structure leads to the symptoms described in Cockayne syndrome.[39]
In xeroderma pigmentosa, the XPD helicase mutation exists at the site of ATP or DNA binding.[39] This results in a structurally functional helicase able to facilitate transcription, however it inhibits its function in unwinding DNA and DNA repair.[39] The lack of cell's ability to repair mutations, such as those caused by sun damage, is the cause of the high cancer rate in xeroderma pigmentosa patients.
RecQ family mutations[edit]
RecQ helicases (3'-5') belong to the Superfamily II group of helicases, which help to maintain stability of the genome and suppress inappropriate recombination.[44][45] Deficiencies and/or mutations in RecQ family helicases display aberrant genetic recombination and/or DNA replication, which leads to chromosomal instability and an overall decreased ability to proliferate.[44] Mutations in RecQ family helicases BLM, RECQL4, and WRN, which play a role in regulating homologous recombination, have been shown to result in the autosomal recessive diseases Bloom syndrome (BS), Rothmund-Thomson syndrome (RTS), and Werner syndrome (WS), respectively.[45][46]
Bloom syndrome is characterized by a predisposition to cancer with early onset, with a mean age-of-onset of 24 years.[45][47] Cells of Bloom syndrome patients show a high frequency of reciprocal exchange between sister chromatids (SCEs) and excessive chromosomal damage.[48] There is evidence to suggest that BLM plays a role in rescuing disrupted DNA replication at replication forks.[48]
Werner syndrome is a disorder of premature aging, with symptoms including early onset of atherosclerosis and osteoporosis and other age related diseases, a high occurrence of sarcoma, and death often occurring from myocardial infarction or cancer in the 4th to 6th decade of life.[45][49] Cells of Werner syndrome patients exhibit a reduced reproductive lifespan with chromosomal breaks and translocations, as well as large deletions of chromosomal components, causing genomic instability.[49]
Rothmund-Thomson syndrome, also known as poikiloderma congenitale, is characterized by premature aging, skin and skeletal abnormalities, rash, poikiloderma, juvenile cataracts, and a predisposition to cancers such as osteosarcomas.[45][50] Chromosomal rearrangements causing genomic instability are found in the cells of Rothmund-Thomson syndrome patients.[50]
RNA helicases[edit]
RNA helicases are essential for most processes of RNA metabolism such as ribosome biogenesis, pre-mRNA splicing, and translation initiation. They also play an important role in sensing viral RNAs.[51] RNA helicases are involved in the mediation of antiviral immune response because they can identify foreign RNAs in vertebrates. About 80% of all viruses are RNA viruses and they contain their own RNA helicases.[52] Defective RNA helicases have been linked to cancers, infectious diseases and neuro-degenerative disorders.[51] Some neurological disorders associated with defective RNA helicases are: amyotrophic lateral sclerosis, spinal muscular atrophy, spinocerebellar ataxia type-2, Alzheimer disease, and lethal congenital contracture syndrome.[52]
RNA helicases and DNA helicases can be found together in all the helicase superfamilies except for SF6.[53][54] All the eukaryotic RNA helicases that have been identified up to date are non-ring forming and are part of SF1 and SF2. On the other hand, ring-forming RNA helicases have been found in bacteria and viruses.[51] However, not all RNA helicases exhibit helicase activity as defined by enzymatic function, i.e., proteins of the Swi/Snf family. Although these proteins carry the typical helicase motifs, hydrolize ATP in a nucleic acid-dependent manner, and are built around a helicase core, in general, no unwinding activity is observed.[55]
RNA helicases that do exhibit unwinding activity have been characterized by at least two different mechanisms: canonical duplex unwinding and local strand separation. Canonical duplex unwinding is the stepwise directional separation of a duplex strand, as described above, for DNA unwinding. However, local strand separation occurs by a process wherein the helicase enzyme is loaded at any place along the duplex. This is usually aided by a single-strand region of the RNA, and the loading of the enzyme is accompanied with ATP binding.[56] Once the helicase and ATP are bound, local strand separation occurs, which requires binding of ATP but not the actual process of ATP hydrolysis.[57] Presented with fewer base pairs the duplex then dissociates without further assistance from the enzyme. This mode of unwinding is used by the DEAD/DEAH box helicases.[58]
An RNA helicase database is currently available online that contains a comprehensive list of RNA helicases with information such as sequence, structure, and biochemical and cellular functions.[51]
Diagnostic tools for helicase measurement[edit]
Measuring and monitoring helicase activity[edit]
Various methods are used to measure helicase activity in vitro. These methods range from assays that are qualitative (assays that usually entail results that do not involve values or measurements) to quantitative (assays with numerical results that can be utilized in statistical and numerical analysis). In 1982-1983, the first direct biochemical assay was developed for measuring helicase activity.[13][59] This method was called a "strand displacement assay".
- Strand displacement assay involves the radiolabeling of DNA duplexes. Following helicase treatment, the single-strand DNA is visually detected as separate from the double-strand DNA by non-denaturing PAGE electrophoresis. Following detection of the single-strand DNA, the amount of radioactive tag that is on the single-strand DNA is quantified to give a numerical value for the amount of double-strand DNA unwinding.
- The strand displacement assay is acceptable for qualitative analysis, its inability to display results for more than a single time point, its time consumption, and its dependence on radioactive compounds for labeling warranted the need for development of diagnostics that can monitor helicase activity in real time.
Other methods were later developed that incorporated some, if not all of the following: high-throughput mechanics, the use of non-radioactive nucleotide labeling, faster reaction time/less time consumption, real-time monitoring of helicase activity (using kinetic measurement instead of endpoint/single point analysis). These methodologies include: "a rapid quench flow method, fluorescence-based assays, filtration assays, a scintillation proximity assay, a time resolved fluorescence resonance energy transfer assay, an assay based on flashplate technology, homogenous time-resolved fluorescence quenching assays, and electrochemiluminescence-based helicase assays".[14] With the use of specialized mathematical equations, some of these assays can be utilized to determine how many base paired nucleotides a helicase can break per hydrolysis of 1 ATP molecule.[60]
Commercially available diagnostic kits are also available. One such kit is the "Trupoint" diagnostic assay from PerkinElmer, Inc. This assay is a time-resolved fluorescence quenching assay that utilizes the PerkinEmer "SignalClimb" technology that is based on two labels that bind in close proximity to one another but on opposite DNA strands. One label is a fluorescent lanthanide chelate, which serves as the label that is monitored through an adequate 96/384 well plate reader. The other label is an organic quencher molecule. The basis of this assay is the "quenching" or repressing of the lanthanide chelate signal by the organic quencher molecule when the two are in close proximity - as they would be when the DNA duplex is in its native state. Upon helicase activity on the duplex, the quencher and lanthanide labels get separated as the DNA is unwound. This loss in proximity negates the quenchers ability to repress the lanthanide signal, causing a detectable increase in fluorescence that is representative of the amount of unwound DNA and can be used as a quantifiable measurement of helicase activity.
Determining helicase polarity[edit]
Helicase polarity, which is also deemed "directionality", is defined as the direction (characterized as 5'→3' or 3'→5') of helicase movement on the DNA/RNA single-strand along which it is moving. This determination of polarity is vital in f.ex. determining whether the tested helicase attaches to the DNA leading strand, or the DNA lagging strand. To characterize this helicase feature, a partially duplex DNA is used as the substrate that has a central single-strand DNA region with different lengths of duplex regions of DNA (one short region that runs 5'→3' and one longer region that runs 3'→5') on both sides of this region.[61] Once the helicase is added to that central single-strand region, the polarity is determined by characterization on the newly formed single-strand DNA.
See also[edit]
- Chromodomain helicase DNA binding protein: CHD1, CHD1L, CHD2, CHD3, CHD4, CHD5, CHD6, CHD7, CHD8, CHD9
- DEAD box/DEAD/DEAH box helicase: DDX3X, DDX5, DDX6, DDX10, DDX11, DDX12, DDX58, DHX8, DHX9, DHX37, DHX40, DHX58
- ^ Wu Y (2012). "Unwinding and rewinding: double faces of helicase?". J Nucleic Acids. 2012: 140601. doi:10.1155/2012/140601. PMC 3409536. PMID 22888405.
- ^ Umate P, Tuteja N, Tuteja R (January 2011). "Genome-wide comprehensive analysis of human helicases". Commun Integr Biol. 4 (1): 118–37. doi:10.4161/cib.4.1.13844 (inactive 2017-01-30). PMC 3073292. PMID 21509200.
- ^ a b Patel, S. S.; Donmez, I (2006). "Mechanisms of Helicases". Journal of Biological Chemistry. 281 (27): 18265–18268. doi:10.1074/jbc.R600008200. ISSN 0021-9258. PMID 16670085.
- ^ Lionnet T, Spiering MM, Benkovic SJ, Bensimon D, Croquette V (2007). "Real-time observation of bacteriophage T4 gp41 helicase reveals an unwinding mechanism". PNAS. 104 (50): 19790–19795. Bibcode:2007PNAS..10419790L. doi:10.1073/pnas.0709793104. PMC 2148377. PMID 18077411.
- ^ Johnson DS, Bai L, Smith BY, Patel SS, Wang MD (2007). "Single-molecule studies reveal dynamics of DNA unwinding by the ring-shaped t7 helicase". Cell. 129 (7): 1299–309. doi:10.1016/j.cell.2007.04.038. PMC 2699903. PMID 17604719.
- ^ a b "Researchers solve mystery of how DNA strands separate". 2007-07-03. Retrieved 2007-07-05.
- ^ a b c d e f g h i j k Manosas M, Xi XG, Bensimon D, Croquette V (September 2010). "Active and passive mechanisms of helicases". Nucleic Acids Res. 38 (16): 5518–26. doi:10.1093/nar/gkq273. PMC 2938219. PMID 20423906.
- ^ Wu, C. G. and Spies, M.: Overview: What are Helicases? In: Spies, M. (Ed.): [1]. Springer Science+Business Media, NY, 2013
- ^ Highlights DNA Replication
- ^ 3-D Animation Library; Replication: [2] (Advanced)
- ^ a b Abdel-Monem M, Dürwald H, Hoffmann-Berling H (June 1976). "Enzymic unwinding of DNA. 2. Chain separation by an ATP-dependent DNA unwinding enzyme". Eur. J. Biochem. 65 (2): 441–9. doi:10.1111/j.1432-1033.1976.tb10359.x. PMID 133023.
- ^ a b Hotta Y, Stern H (May 1978). "DNA unwinding protein from meiotic cells of Lilium". Biochemistry. 17 (10): 1872–80. doi:10.1021/bi00603a011. PMID 207302.
- ^ a b c Venkatesan M, Silver LL, Nossal NG (October 1982). "Bacteriophage T4 gene 41 protein, required for the synthesis of RNA primers, is also a DNA helicase.". J. Biol. Chem. 257 (20): 12426–34. PMID 6288720.
- ^ a b Tuteja N, Tuteja R (May 2004). "Prokaryotic and eukaryotic DNA helicases. Essential molecular motor proteins for cellular machinery". Eur. J. Biochem. 271 (10): 1835–48. doi:10.1111/j.1432-1033.2004.04093.x. PMID 15128294.
- ^ Hubscher U, Stalder HP (1985). "Mammalian DNA helicase". Nucleic Acids Res. 13 (15): 5471–5483. doi:10.1093/nar/13.15.5471. PMC 321884. PMID 3162158.
- ^ Stahl H, Dröge P, Knippers R (August 1986). "DNA helicase activity of SV40 large tumor antigen". EMBO J. 5 (8): 1939–44. PMC 1167061. PMID 3019672.
- ^ Sugino A, Ryu BH, Sugino T, Naumovski L, Friedberg EC (September 1986). "A new DNA-dependent ATPase which stimulates yeast DNA polymerase I and has DNA-unwinding activity". J. Biol. Chem. 261 (25): 11744–50. PMID 3017945.
- ^ Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM (June 1989). "Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes". Nucleic Acids Res. 17 (12): 4713–30. doi:10.1093/nar/17.12.4713. PMC 318027. PMID 2546125.
- ^ Linder, P., Lasko, P.F., Ashburner, M., Leroy, P., Nielson, P.J., Nishi, K., Schneir, J., Slonimski, P.P. (1989) Birth of the DEAD-box. Nature (London) 337, 121-122.
- ^ Tuteja N, Tuteja R, Rahman K, Kang LY, Falaschi A (December 1990). "A DNA helicase from human cells". Nucleic Acids Res. 18 (23): 6785–92. doi:10.1093/nar/18.23.6785. PMC 332732. PMID 1702201.
- ^ Hehman GL, Hauswirth WW (September 1992). "DNA helicase from mammalian mitochondria". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8562–6. Bibcode:1992PNAS...89.8562H. doi:10.1073/pnas.89.18.8562. PMC 49960. PMID 1326759.
- ^ Tuteja N, Phan TN, Tewari KK (May 1996). "Purification and characterization of a DNA helicase from pea chloroplast that translocates in the 3'-to-5' direction". Eur. J. Biochem. 238 (1): 54–63. doi:10.1111/j.1432-1033.1996.0054q.x. PMID 8665952.
- ^ Tuteja R, Malhotra P, Song P, Tuteja N, Chauhan VS (2002). "Isolation and characterization of an eIF-4A homologue from Plasmodium cynomolgi". Mol. Biochem. Parasitol. 124 (1–2): 79–83. doi:10.1016/S0166-6851(02)00205-0. PMID 12387853.
- ^ a b c d e f g h i j k l m n o p Martin Singleton; Mark S. Dillingham; Dale B. Wigley (2007). "Structure and mechanism of Helicases and Nucleic Acid Translocases". Annual Review of Biochemistry. 76: 23–50. doi:10.1146/annurev.biochem.76.052305.115300. PMID 17506634.
- ^ a b Margaret E. Fairman-Williams; Ulf-Peter Guenther; Echard Jankowsky (2010). "SF1 and SF2 helicases: family matters". Current Opinion in Structural Biology. 20 (3): 313–324. doi:10.1016/j.sbi.2010.03.011. PMC 2916977. PMID 20456941.
- ^ Stelter M, Acajjaoui S, McSweeney S, Timmins J (2013). "Structural and Mechanistic Insight into DNA Unwinding by Deinococcus radiodurans UvrD". PLoS ONE. 8 (10): :e77364. Bibcode:2013PLoSO...877364S. doi:10.1371/journal.pone.0077364. PMC 3797037. PMID 24143224.
- ^ a b Pavan Umate; Narendra Tuteja; Renu Tuteja (2011). "Genome-wide comprehensive análisis of human helicases". Communicative & Integrative Biology. 4 (1): 118–137. doi:10.4161/cib.4.1.13844 (inactive 2017-01-30). PMC 3073292. PMID 21509200.
- ^ Koonin EV, Aravind L, Iyer LM (2001). "Common origin of four diverse families of large eukaryotic DNA viruses". J. Virol. 75 (23): 11720–34. doi:10.1128/JVI.75.23.11720-11734.2001. PMC 114758. PMID 11689653.
- ^ Koonin EV, Aravind L, Leipe DD, Iyer LM (2004). "Evolutionary history and higher order classification of AAA+ ATPases". J. Struct. Biol. 146 (1–2): 11–31. doi:10.1016/j.jsb.2003.10.010. PMID 15037234.
- ^ a b c d e Ropers HH, Hamel BC (January 2005). "X-linked mental retardation". Nat. Rev. Genet. 6 (1): 46–57. doi:10.1038/nrg1501. PMID 15630421.
- ^ Gibbons RJ, Picketts DJ, Villard L, Higgs DR (March 1995). "Mutations in a putative global transcriptional regulator cause X-linked mental retardation with alpha-thalassemia ATR-X syndrome". Cell. 80 (6): 837–45. doi:10.1016/0092-8674(95)90287-2. PMID 7697714.
- ^ a b Nextrprot Online Protein Database. " ATRX-Transcriptional regulator ATRX.", Retrieved on 12 November 2012.
- ^ a b Picketts DJ, Higgs DR, Bachoo S, Blake DJ, Quarrell OW, Gibbons RJ (December 1996). "ATRX encodes a novel member of the SNF2 family of proteins: mutations point to a common mechanism underlying the ATR-X syndrome". Hum. Mol. Genet. 5 (12): 1899–907. doi:10.1093/hmg/5.12.1899. PMID 8968741.
- ^ a b c Gibbons R (2006). "Alpha thalassaemia-mental retardation, X linked". Orphanet J Rare Dis. 1: 15. doi:10.1186/1750-1172-1-15. PMC 1464382. PMID 16722615.
- ^ Pagon RA, Bird TD, Dolan CR, Stephens K, Adam MP, Stevenson RE (1993). "Alpha-Thalassemia X-Linked Intellectual Disability Syndrome". PMID 20301622.
- ^ a b Gibbons RJ, Picketts DJ, Villard L, Higgs DR (March 1995). "Mutations in a putative global transcriptional regulator cause X-linked mental retardation with alpha-thalassemia (ATR-X syndrome)". Cell. 80 (6): 837–45. doi:10.1016/0092-8674(95)90287-2. PMID 7697714.
- ^ Singleton MR, Dillingham MS, Wigley DB (2007). "Structure and mechanism of helicases and nucleic acid translocases". Annu. Rev. Biochem. 76: 23–50. doi:10.1146/annurev.biochem.76.052305.115300. PMID 17506634.
- ^ Rudolf J, Rouillon C, Schwarz-Linek U, White MF (January 2010). "The helicase XPD unwinds bubble structures and is not stalled by DNA lesions removed by the nucleotide excision repair pathway". Nucleic Acids Res. 38 (3): 931–41. doi:10.1093/nar/gkp1058. PMC 2817471. PMID 19933257.
- ^ a b c d e f g h i j k l m n o Fan L, Fuss JO, Cheng QJ, Arvai AS, Hammel M, Roberts VA, Cooper PK, Tainer JA (May 2008). "XPD helicase structures and activities: insights into the cancer and aging phenotypes from XPD mutations". Cell. 133 (5): 789–800. doi:10.1016/j.cell.2008.04.030. PMC 3055247. PMID 18510924.
- ^ a b Lainé JP, Mocquet V, Egly JM (2006). "TFIIH enzymatic activities in transcription and nucleotide excision repair". Meth. Enzymol. Methods in Enzymology. 408: 246–63. doi:10.1016/S0076-6879(06)08015-3. ISBN 9780121828134. PMID 16793373.
- ^ a b Tirode F, Busso D, Coin F, Egly JM (January 1999). "Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7". Mol. Cell. 3 (1): 87–95. doi:10.1016/S1097-2765(00)80177-X. PMID 10024882.
- ^ a b Sung P, Bailly V, Weber C, Thompson LH, Prakash L, Prakash S (October 1993). "Human xeroderma pigmentosum group D gene encodes a DNA helicase". Nature. 365 (6449): 852–5. Bibcode:1993Natur.365..852S. doi:10.1038/365852a0. PMID 8413672.
- ^ a b Schaeffer L, Roy R, Humbert S, Moncollin V, Vermeulen W, Hoeijmakers JH, Chambon P, Egly JM (April 1993). "DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor". Science. 260 (5104): 58–63. Bibcode:1993Sci...260...58S. doi:10.1126/science.8465201. PMID 8465201.
- ^ a b Hanada K, Hickson ID (September 2007). "Molecular genetics of RecQ helicase disorders". Cell. Mol. Life Sci. 64 (17): 2306–22. doi:10.1007/s00018-007-7121-z. PMID 17571213.
- ^ a b c d e Opresko PL, Cheng WH, Bohr VA (April 2004). "Junction of RecQ helicase biochemistry and human disease". J. Biol. Chem. 279 (18): 18099–102. doi:10.1074/jbc.R300034200. PMID 15023996.
- ^ Ouyang KJ, Woo LL, Ellis NA (2008). "Homologous recombination and maintenance of genome integrity: cancer and aging through the prism of human RecQ helicases". Mech. Ageing Dev. 129 (7–8): 425–40. doi:10.1016/j.mad.2008.03.003. PMID 18430459.
- ^ Ellis NA, Groden J, Ye TZ, Straughen J, Lennon DJ, Ciocci S, Proytcheva M, German J (November 1995). "The Bloom's syndrome gene product is homologous to RecQ helicases". Cell. 83 (4): 655–66. doi:10.1016/0092-8674(95)90105-1. PMID 7585968.
- ^ a b Selak N, Bachrati CZ, Shevelev I, Dietschy T, van Loon B, Jacob A, Hübscher U, Hoheisel JD, Hickson ID, Stagljar I (September 2008). "The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta". Nucleic Acids Res. 36 (16): 5166–79. doi:10.1093/nar/gkn498. PMC 2532730. PMID 18682526.
- ^ a b Gray MD, Shen JC, Kamath-Loeb AS, Blank A, Sopher BL, Martin GM, Oshima J, Loeb LA (September 1997). "The Werner syndrome protein is a DNA helicase". Nat. Genet. 17 (1): 100–3. doi:10.1038/ng0997-100. PMID 9288107.
- ^ a b Kitao S, Shimamoto A, Goto M, Miller RW, Smithson WA, Lindor NM, Furuichi Y (May 1999). "Mutations in RECQL4 cause a subset of cases of Rothmund-Thomson syndrome". Nat. Genet. 22 (1): 82–4. doi:10.1038/8788. PMID 10319867.
- ^ a b c d Jankowsky, A.; Guenther, U. -P.; Jankowsky, E. (2010). "The RNA helicase database". Nucleic Acids Research. 39 (Database issue): D338–D341. doi:10.1093/nar/gkq1002. PMC 3013637. PMID 21112871.
- ^ Jankowsky E, Fairman-Williams ME (2010). "An introduction to RNA helicases: superfamilies, families, and major themes". In Jankowsky E. RNA Helicases (RSC Biomolecular Sciences). Cambridge, England: Royal Society of Chemistry. p. 5. ISBN 1-84755-914-X.
- ^ Ranji, A.; Boris-Lawrie, K. (2010). "RNA helicases: Emerging roles in viral replication and the host innate response". RNA Biology. 7 (6): 775–787. doi:10.4161/rna.7.6.14249. PMC 3073335. PMID 21173576.
- ^ Jankowsky E (January 2011). "RNA helicases at work: binding and rearranging". Trends Biochem. Sci. 36 (1): 19–29. doi:10.1016/j.tibs.2010.07.008. PMC 3017212. PMID 20813532.
- ^ Yang Q, Del Campo M, Lambowitz AM, Jankowsky E (October 2007). "DEAD-box proteins unwind duplexes by local strand separation". Mol. Cell. 28 (2): 253–63. doi:10.1016/j.molcel.2007.08.016. PMID 17964264.
- ^ Liu F, Putnam A, Jankowsky E (December 2008). "ATP hydrolysis is required for DEAD-box protein recycling but not for duplex unwinding". Proc. Natl. Acad. Sci. U.S.A. 105 (51): 20209–14. Bibcode:2008PNAS..10520209L. doi:10.1073/pnas.0811115106. PMC 2629341. PMID 19088201.
- ^ Jarmoskaite I, Russell R (2011). "DEAD-box proteins as RNA helicases and chaperones". Wiley Interdiscip Rev RNA. 2 (1): 135–52. doi:10.1002/wrna.50. PMC 3032546. PMID 21297876.
- ^ Matson SW, Tabor S, Richardson CC (November 1983). "The gene 4 protein of bacteriophage T7. Characterization of helicase activity". J. Biol. Chem. 258 (22): 14017–24. PMID 6315716.
- ^ Sarlós K, Gyimesi M, Kovács M (June 2012). "RecQ helicase translocates along single-stranded DNA with a moderate processivity and tight mechanochemical coupling". Proc. Natl. Acad. Sci. U.S.A. 109 (25): 9804–9. Bibcode:2012PNAS..109.9804S. doi:10.1073/pnas.1114468109. PMC 3382518. PMID 22665805.
- ^ Borowiec, J. (1996) DNA Replication in Eukaryotic Cells. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 545-574
External links[edit]
Wikimedia Commons has media related to Helicases. |
- DNA Helicases at the US National Library of Medicine Medical Subject Headings (MeSH)
- RNA Helicases at the US National Library of Medicine Medical Subject Headings (MeSH)


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OK, not done one before so this is new ground to cover.
No particular reasons for these questions, just maybe some variations on what we have done before.
1) I am a big fan of what literature geeks call genre fiction. I love fantasy, science fiction, historical novels, detective stuff, so the first question is;
What is your favourite fiction genre and what book is the epitome of that style for you?
2) I think we have done musical guilty pleasures before, but it is always worth revisiting. Recently I bought Seals and Crofts Diamond Girl album, it was a fave of mine back in the mid '70s. What is your most recent gulity pleasure music purchase?
3) A food question. What is the earliest "exotic" thing you remember eating that was something completely outside of what you had at home?
4) I am a big fan of Marvel comics and my favourite character is Doctor Strange (hence the pic). Do you like comics and, if so, who is your fave character?
5) Last one. Have you ever wanted to be a character in a novel? If so, who and why?


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Healthy Mouth, Healthy Kids |
Diagnosis of Ankylosed Tooth08/13/2009 |
Is it possible to diagnose a tooth as ankylosed simply by looking at an x-ray? A 6-year molar in my 14 year old`s mouth has not erupted - 2 years ago a cyst above that tooth was removed by an oral surgeon. One orthodontist wants to extract the tooth, claiming it is ankylosed simply by looking at an x-ray. Another orthodontist states that the molar is impacted and would like to have the tooth bonded and attempt to pull it up - the second orthodontist believes that if the molar then does not move, it is ankylosed. So can an ankylosed tooth be diagnosed by looking at an x-ray?
Ankylosis can be diagnosed typically when one tooth appears 'submerged' in relation to the neighboring teeth on an xray. Practitioners will also use percussion (ankylosed teeth make a characteristic metallic sound), but these are subjective. Remember that an xray is a two-dimensional image of a three-dimensional object. In some cases, there is simply a single point of ankylosis along the entire root surface, and in some cases pressure on the tooth can cause movement. It is also possible that the cyst somehow impacted the tooth development.
You might want to consult with an oral and maxillofacial surgeon who might use some additional imaging to make a diagnosis. I hope this helps.
Sarath Thikkurissy, DDS, MS Professor Director, Residency Program, Division of Pediatric Dentistry and Orthodontics College of Medicine University of Cincinnati |


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Broadway water supplies back after days
- 4 December 2012
- From the section Hereford & Worcester
Water supplies have been restored to residents in a Worcestershire village - 10 days after they were cut off because of fears they were polluted.
Severn Trent officials had to hand out bottled water to people in Broadway after the water became discoloured and was found to have an unusual taste.
Engineers found a cracked pipe was letting in standing water and polluting the system. The firm has apologised.
The company said water supplies returned at about 22:00 GMT on Monday.
Officials said all customers had now received a letter saying the water was safe to drink.
Company representatives were also due to be outside the village's Horse and Hound Inn on Tuesday to answer any questions.
Customers will be credited with £25.