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Design a Logo
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Project Description
I would like to have a logo for my business which is in the Permanent MakeUp industry.
The title will be "Val's"
the sub title will be "perfection made easy"
I would like the logo to have an expensive look, impressive, something luxuries with a touch of fun.
Tell me your ideas or better show me a rough sketch.
Good love for ya all!
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Ahmadinejad Phenomenon and The Government of Chaos!
Picture: Ahmadinejad and Said Mortazavi
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No one knows what to do with Ahmadinejad .Not only he feels that he is above the parliament and law ,he even shows that he is above Khamenei the leader of the Islamic Republic ( his 11 days absent from attending cabinet meeting on dispute with Khamenei about the resignation of the Minister of Information) .During seven out of eight years of his presidency his Government had more than $600 billion income just from oil revenue and no one knows what he did with the money.According to news, the Central Bank reserve went down to zero and it even seems negative and inflation rose to its highest level. His Government owes billions of Tuman to different institution ( like metro ) as well as ministries. During his Government, corruption went up and in one instance according to report and investigations 3000 billion Tuman ( $1 = 2000 Tuman)vanished from the banks. He is a bully and all around him are thugs . One of such is the former judge Said Mortazavi the suspected muderer of Iranian -Canadian photojournalist Zahra Kazemi and Kahrizak prison case file where some political prisoners were murdered under his direct order. Recently Ahmadinejad nominated Mortazavi to a new post as the head of social security organization and this angered alot of MP's . dispute over Ahmadinejad continues as the MP's called him to the parliament for questiening over and over now.No one knows who lead Ahmadinejad but one thing is clear the captain is driving the country to more disaster.....The situation is mixed up......

subset: commoncrawl


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O.k. so it looks like on an ungrounded system the current through the capacitance coupling is still enough to kill. The current runs through the body and returns on the capacitance coupling of the other two phases.
Did you simply connect your wiggy between one of the phases and ground?
Is 500ohms a typical value that is used for the human body for such rough calculations? I thought I have seen this number used somewhere before?
So using this resistnace value the maximum current that will flow through the body at 277V will be about .554A? I have heard others state that 100's of amps would flow through body which I never chose to believe.
Does the impedance of the system then only come into play for a bolted fault condition? For such faults with a fault resistance there is no need to consider upstream impedance? Therefore shock would be that same at 480V MCC bus as it would at a 480V lighting circuit?


text: I'm a world traveler, attended Haas UC Berkeley, originally from Delray Beach, Florida, where we have condos available. My husband and I live and work right near the Walnut apartments. We take pride in our properties and are responsive to any of our renters needs. Since we are local, we deal with our guests directly – from key exchanges, to cleaning, to repairs, and anything in between. All of our properties are fully furnished and have all the amenities of home!


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THIS RESTAURANT IS CLOSED Fat City Steakhouse
2137 Pacific Hwy. (Hawthorne St.) Send to Phone
Dinner nightlyFeatures
- Parking lot
- Dress code: Casual dressy
- Full bar
- Heart-healthy dishes
- Kid-friendly
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* Click here for rating key
THIS RESTAURANT IS CLOSED Fat City Steakhouse Restaurant Review: If you are anywhere near the airport, you can't miss the big pink building on Pacific Coast Highway that houses Fat City Steakhouse. This isn't your upper crust, à la carte type of steakhouse; rather, it offers good, more affordable food that folks return for again and again. The majority of steaks are USDA Choice, with one Prime cut in the bunch. There are even a few chicken, seafood and vegetarian dishes for those who eschew red meat. Fat City is an ideal place to bring the kids, grab a quick bite before traveling or throw a raucous birthday party with a group of friends. |


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Suzuki GEO All Discussion of all things, Suzuki and GEO |
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What carb??
Hey guys, its been a looooooong time since I was last on the board.. I'm in the process of sprucing up the old zuk for driving to work and back.. Nothing fast and nothing rougher than maybe a gravel parking lot.. Shes old and in not so good shape but she runs and drives ok for a zuki.. The factory carb is still in place and its shot.. I've been reading some about the CV Harley carbs.. Has anyone gotten one to work correctly?? Or is there something out there that is just as easy and cheap..I'm just so tired of reading and being so confused over the whole thing.. I need to get by on the cheap cause there are other things I would like to do to it.. Thanks..
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Hong Kong, 20 February 2012
On 18 February, Foxconn Technology Group announced a pay rise for the production workers in China. The new basic wage at CNY 1800 only applies to the workers in Shenzhen. In inland provinces, where two-third productions workers are based, basic salary remains meager. Given that the inflation in China is high, Foxconn is just following the trend of wage increase in the electronics industry in China.
Pay rise is a trend in Guangdong Province
According to the Shenzhen research team of the National Bureau of Statistics, the Consumer Price Index (CPI) for food in January 2012 rose 10% over the last year, of which price for pork increased by 30%. Apparently, the low-income migrant workers are disproportionately affected by the inflation. As a result, there is a strong demand from workers to increase the basic salary. Moreover, most of the migrant workers can only visit their family once a year during the Chinese New Year. In the past few years, there are more job opportunities in the inland provinces, more workers prefer to seek jobs in their home provinces. The enterprises in coastal area worry about the shortage of labour. Therefore, many companies tend to increase the basic salary to attract workers. As such, Foxconn is not the only enterprise which raises the basic salary of workers. Other electronics factories, like Huawei is also paying CNY 1800 a month for new workers. In Dongguan, many electronics factories like Flextronics and Wintek are paying CNY 1400 a month to new workers. The amount is about 27% higher than the minimum wage. In other words, Foxconn increases the salary is not out of benevolence, but the needs to maintain stability of the workforce.
Wages in most of the Foxconn plants remain low
Foxconn has over 1.2 million workers in China. The workforce in Shenzhen is estimated at 400,000. Workers in inland province receive a much lower salary. For example, Foxconn workers in Chengdu and Zhengzhou who produce iPads and iPhones, their basic salary is CNY 1350-1550 per month. After deducting CNY 110-150 for the dormitory and about CNY 300 for food per month, the disposable income remains meager.
Wages of Foxconn workers in Shenzhen, Chengdu and Zhengzhou (in CNY):
Shenzhen |
Chengdu |
Zhengzhou |
Minimum Wage |
1500 |
1050 |
1080 |
Basic Salary |
1800 |
1550 |
1350 |
Basic Salary after 9 months |
2200 – 2500 |
1750 |
1650 |
Since Apple has bought a membership from the Fair Labour Association, it seems that Apple becomes transparent and socially responsible. Apple is the world's most valuable brand. It can definitely afford a living wage for the workers. Without raising unit price, workers still have to toil long hours in the production lines to earn a living from the overtime premiums. Yet, the FLA will not pressure its members to raise the unit price to provide a living wage for the workers. To fight for a living wage, workers should have the right to form union by democratic elections. At the same time, consumers should keep up the pressure on Apple.
Please find the PDF version of the statement here.


text: No Wake On Charge Keeps Your Samsung Screen Off When Plugged InMark Wilson7/29/14 7:30amFiled to: downloadsAndroid DownloadsAndroidRootedSamsungxposed39EditPromoteShare to KinjaToggle Conversation toolsGo to permalinkAndroid (Rooted): When you plug in your phone to power up overnight, you've probably noticed that the screen lights up. If you have a Samsung Galaxy S5, S4, or S3, here's how to stop the screen from turning on when you plug it in. No Wake On Charge serves a simple function but it's one that many people will welcome. It's an Xposed module that needs the Xposed Framework which we have talked about before. Click the download link below to get hold of the module, enable it in Xposed and restart your handset. That's all there is to it. No more blinding at night, and no more rushing to hit the power button, just simple, no-fuss charging. The module claims to work only with Samsung Galaxy S5s, but the WonderHowTo video claims success with S3 and S4 handsets as well—my own tests with an S4 were successful. Advertisement Advertisement No Wake On Charge (Free) |Xposed Repository via WonderHowToReply39 repliesLeave a reply


text:
NADase as a target molecule of in vivo suppression of the toxicity in the invasive M-1 group A Streptococcal isolates
- Ichiro Tatsuno1,
- Masanori Isaka1,
- Masaaki Minami1 and
- Tadao Hasegawa1Email author
© Tatsuno et al; licensee BioMed Central Ltd. 2010
Received: 19 May 2009
Accepted: 17 May 2010
Published: 17 May 2010
NAD-glycohydrolase (NADase) secreted by M-1 group A streptococcal (GAS) isolates are suspected as one of the virulence factors to cause severe invasive disease including streptococcal toxic shock-like syndrome (STSS). M-1 GAS strains were divided into three groups based on NADase activity: high activity, low activity and no activity in our previous report.
The representative high activity isolates taken from STSS patients showed higher virulence compared with isolates from the low activity group, when used to infect mice. The knockout mutant of the nga gene, which encodes NADase also showed reduced virulence in a mouse infection study. The cloned nga gene was able to significantly complement the lost virulence. In addition, the solution containing purified recombinant IFS, which is an inhibitor of NADase, partially rescued mice infected with S. pyogenes.
These results indicate that NADase is important for the virulence of S. pyogenes in vivo and is the potential target to suppress the virulence.
Group A streptococcus (GAS) is a gram-positive bacterium that infects the upper respiratory tract, including the tonsils and pharynx, and is responsible for post-infectious diseases such as rheumatic fever and glomerulonephritis. In addition, GAS causes severe invasive disease including necrotizing fasciitis [1–6].
Although the mechanism of severe invasive disease is still unknown, NAD-glycohydrolase (NADase) secreted by GAS is suspected as one of the virulence factors [7]. NADase has the ability to cleave β-NAD+, which is universally important in numerous essential redox and energy-producing biological reactions, depleting intracellular NAD pools [8, 9]. NADase is also toxic for bacterial cells themselves, therefore, GAS encodes ifs gene whose product (IFS) is an endogenous inhibitor of NADase activity and localized in the bacterial cytoplasmic compartment [9, 10]. NADase precursor exists as an inactive complex with IFS [9, 10]. In vitro, intoxication of keratinocytes with NADase was associated with cytotoxic effects [11, 12]. Bricker et al. presented that NADase enhances GAS virulence in vivo using mouse models [13]. These results enabled us to further study the NADase as a target molecule to reduce GAS virulence. However, another study of GAS infection among aboriginal people in Australia found no relationship between NADase production and severity or outcome of GAS infection [14]. Furthermore, we recently reported that M-1 group A streptococcal isolates were divided into three groups based on NADase activity: high activity, low activity and no activity [15], whereas we did not find that low and high levels of the NADase activity correlated with severity of GAS human infection (data not shown). Meanwhile, Ajdic et al. reported that among 73 strains isolated from patients with mostly invasive GAS infections from a recent outbreak of streptococcal infection, 67 (92%) were NADase producer [16], although strains isolated from patients with non-invasive GAS infections were not assayed. It is unknown why the 8% strains isolated from patients with mostly invasive GAS infections were not NADase producer. Therefore, we thought that before taking up the study of our interest, it should be further determined how NADase is important as a virulence factor for severe invasive disease. We mainly focused on the following two points: (i) How do NADase activity levels correlate with virulence? (ii) If NADase is important for severe invasive disease, and whether it is possible that IFS suppresses the severity. In this study, we present further evidences to prove the importance of NADase in severe invasive disease.
Bacterial strains
M-1GAS clinical isolates used in this study
Isolates | place# | Isolated year |
SF370 | America | 1985 |
1529 | Japan (Chiba) | 1990-2000 |
KN01 | Japan (Aichi) | 1990-2000 |
MDYK | Japan (Aichi) | 2000 ~ |
MUY | Japan (Mie) | 2000 ~ |
GT01 | Japan (Gunma) | 2000 ~ |
FI01 | Japan (Fukushima) | 2000 ~ |
CR01 | Japan (Aichi) | 2000 ~ |
IYAT | Japan (Fukushima) | 2000 ~ |
Quantitation of NADase activity in bacterial supernatant
NADase activity was determined by the method of Stevens et al. [19] as described previously [15].
Construction of the recombinant His-IFS and His-TarC proteins
The ifs gene of pGST-NgaGT01 (IFS) [15] was amplified by PCR with Extaq DNA polymerase (Takara Bio, Ohtsu, Japan) using primers IFS-F (BamHI) (5'-AGGAAGTAACGGATCC TATAAGGTGC-3') and IFS-R (5'-ATGTGTCAGAGGTTTTCACCG-3'). Oligonucleotide IFS-F(BamHI) contained a restriction site for Bam HI (shown in bold in the primer sequence). The amplification product, which contained a restriction site for Sal I, was digested with Bam HI and Sal I, and cloned into pQE-80L (Qiagen, Hilden, Germany) to yield pHis-IFS, whose insert was sequenced.
Plasmid pHis-TarC encoding a His-tagged carboxyl terminal domain of an Escherichia coli aspartate chemoreceptor (named as His-TarC) was constructed by subcloning a 1.1 kb Kpn I fragment of pIT6 [20] into pQE-80L.
Purification of the recombinant His-tagged proteins
The His-tagged IFS fusion protein was induced and purified under native conditions as described in the manufacture's protocol (Qiagen), with the following modification. To induce the His-IFS fusion protein, 1 mM IPTG was added to a logarithmic-phase culture of E. coli JM109/pHis-IFS and shaken for 3 h at 37°C. A total of 100 ml of the liquid culture was transferred to a centrifuge tube and centrifuged to sediment the cells. The pellet was resuspended in 10 ml ice cold PBS + 1% Triton X-100. After a freeze (-80°C)/thaw and a sonication at 170 W for 2 min (Insonator 201M, Kubota, Tokyo, Japan), insoluble material was removed by spinning it at full speed (16 000 g) for 10 min. One ml of the 50% Ni-NTA slurry was washed twice with 4 ml of Milli-Q water, equilibrated with 1 ml of PBS + 1% Triton X-100, added to the 10 ml cleared lysate and mixed gently by rotating at room temperature for 20 min. The lysate-Ni-NTA mixture was loaded into a column and washed three times with 4 ml wash buffer. The protein was eluted with PBS + 250 mM Imidazole. The protein was verified using SDS-PAGE and anti-RGS-His antibody (Qiagen) or by dose-dependent inhibition of NADase activity of both GAS culture and the GST-Nga fusion protein constructed in a previous report [15].
The His-TarC was induced and purified by the same method described above. In addition, characterization by SDS-PAGE confirmed that the IPTG-dependently induced recombinant protein was purified as essentially a single band of the expected size (31 k Dalton) (data not shown).
Mouse model of invasive skin tissue infection
All animal studies have complied with federal and institutional guidelines. The ability of S. pyogenes to cause local skin lesions and necrosis in mice after skin inoculation was assessed using a procedure similar to that described elsewhere [21]. In brief, 3-week-old female ICR mice (10-12 g) were anesthetized by ketamine-xylazine injection, and the hair was cut from the left flank using scissors and/or electric shaver to bare the skin, unless otherwise indicated. Bacteria (0.1 ml; 1 × 107 cfu per mouse) grown in BHI-Y were injected with a 27-gauge needle just under the surface of the skin so that a superficial bleb was raised immediately below the skin surface. The number of colony-forming units injected was verified for each experiment by plating bacteria on BHI-Y or sheep blood agar plates (with or without kanamycin) and counting colony-forming units.
The purified recombinant His-IFS or His-TarC was injected as follows: (1) on day 0, 25 μg (per 0.1 ml) was inoculated together with bacteria in the left flank. It was confirmed that both His-IFS and His-TarC had no effect on bacterial viability and growth (data not shown), and (2) on days 2-4, 50 μg (per day) was inoculated intraperitoneally. The bacterial viability (and growth) was assessed by incubating the remaining mixture of bacteria and either His-IFS or His-TarC used on the day 0 for 1 to 6 hours, and counting colony-forming units on BHI-Y or sheep blood agar plates. Because it is difficult to increase injection volume in the skin, we decided to increase the concentration of IFS per ml of injection solution. Preliminary test showed highest concentration (no dilution) was more effective at reducing GAS virulence than any of the IFS dilutions tested (data not shown). Thus, we used the highest concentration to add as much IFS as our possible.
Creation of nga mutant of strain GT01
Escherichia coli JM109 was used to propagate plasmid constructions. Non-polar inactivated mutant of nga was constructed via double-crossover allelic replacement in the chromosome of S. pyogenes GT01. To construct the plasmid for the nga knockout mutant, the 5' end of nga (fragment 1) was amplified with oligonucleotide primers ngaGT-n1 (5'-GGCTAGC GAACAGATGTGAAGGTTCTG-3') with an Nhe I restriction site and ngaGT-c1 (5'-TCCCCCGGG TTTCTCATGTAAACCACCT-3') with an Sma I restriction site, and the 3' end of nga (fragment 2) was amplified with ngaGT-n2 (5'-TCCCCCGGG ATAGGAAGTAACAATATGT-3') with an Sma I restriction site and ngaGT-c2 (5'-GGACTAGT ATGTTAGCTTTCAATTGGGT-3') with an Spe I restriction site. Oligonucleotides ngaGT-n1, ngaGT-c1, ngaGT-n2 and ngaGT-c2 contained a restriction site for Nhe I, Sma I, Sma I and Spe I, respectively, (shown in bold in the primer sequence). Fragment 2 was digested with Sma I and Spe I for insertion into multi-cloning site 2 of the pFW12 plasmid [22]. The resulting plasmid was then digested with Nhe I and Sma I, and both the spc2 DNA fragment containing aad9 (promoterless spectinomycin resistant gene), which was obtained from a Sma I digested fragment of pSL60-2 [23], and the Nhe I-Sma I-digested fragment 1 were inserted. This plasmid, nga::aad9/pFW12, was a suicide vector for S. pyogenes. For the preparation of competent cells, strain GT01 was harvested at early- to mid-log phase (OD660 = 0.4 to 0.5) and washed twice with 0.5 M sucrose buffer. The constructed suicide vector nga::aad9/pFW12 was transformed into strain GT01 by electroporation. The conditions of electroporation were 1.25 kV/mm, 25 μF capacitance and 200 Ω resistance, using Gene Pulser II (Bio-Rad, Hercules, CA, USA). After incubation at 37°C for 3 h, competent cells were spread onto BHI agar plates containing 0.3% yeast extract and spectinomycin (final concentration 100 μg/ml). Selected colonies on the plates were cultured. Cultured bacteria were washed once with saline, resuspended in 10 mM Tris, 1 mM EDTA and boiled for 10 min. Genomic DNA was obtained from the supernatant of boiled bacteria. The double-crossover replacement was analyzed using genomic DNA by PCR and successful double-crossover replacement was further confirmed by DNA sequencing.
Cloning of nga gene
All PCR reactions for plasmid construction were undertaken as previously described [15].
The nga GT01 of S. pyogenes strain GT01 was amplified by PCR with Extaq DNA polymerase using primers nga-n4Eco (5'-GGAATTC ATGAGAAACAAAAAAGTAAC-3') and sloC2 (5'-ATCATCCGTTTTCTGACCTG-3') and cloned into pGEM-T easy (Promega, Madison, WI, USA) to yield pNGIe1, whose insert was sequenced. Oligonucleotide nga-n4Eco contained a restriction site for Eco RI endonuclease (shown in bold in the primer sequence). The nga GT01 gene is oriented in the opposite direction as the lacUV5 promoter. An Eco RI fragment containing the nga GT01 gene of pNGIe1 was sub-cloned into pLZ12-Km2 [24] to yield pLZN2, whose insert was sequenced for verification.
To construct pLZN-RBS, inverse PCR with Pyrobest DNA polymerase (Takara) using the primers LZ-R0 (5'-CCGTCGACCTCGAG GGGGGGC-3') and nga-RBS1 (5'-CCGCTCGAG ATATAAGGTGGTTTAC A TG AGAAACAAAAAAGTAAC-3') was performed to add a potential ribosome-binding site (16 bp) to nga encoded on pLZN2. Oligonucleotides nga-RBS1 and LZ-R0 contained a restriction site for Xho I endonuclease, the potential ribosome binding site and/or start codon for the nga gene, respectively (shown in bold, underline and italic in the primer sequence, respectively). The amplification product was digested with Xho I and self-ligated. The insert was sequenced for verification.
To construct pLZN-RBSII2, inverse PCR with PrimeSTAR™ HS DNA polymerase (Takara) using the primers nga-RBS2 (5'-CCGGGGCCCTTAAAAATAATATAAGGTGGTTTAC A TG AG-3') and LZ-R3 (5'-CTCGAGGGGGGGCCCATCAGTC-3') was performed to add the further upstream DNA sequence (10 bp) to the potential ribosome-binding site encoded on pLZN-RBS. A oligonucleotide nga-RBS2 contained the upstream DNA sequence, the potential ribosome binding site and start codon for the nga gene (shown in dotted underline, underline and italic in the primer sequence, respectively). The amplification product was self-ligated and the insert was sequenced for verification.
Statistical analysis
The significant difference of virulence (mortality) between low and high NADase activity groups was ascertained as follows. The mortality of mice infected with each GAS isolate, but not mean mortalities produced by pooling multiple isolates into the two groups, was determined. The four mortalities in the low NADase activity group and the four mortalities in the high NADase activity group were compared using an unpaired t test http://www.graphpad.com/quickcalcs/ttest1.cfm.
Survival times were assessed using a log-rank comparison. R software was used for statistical analysis http://bioinf.wehi.edu.au/software/russell/logrank/. P value ≤ 0.05 was considered significant.
Correlation of NADase activity levels and virulence
Virulence (Mortality) to mouse of GAS isolates with different NADase activity
NADase | Isolate | Mortalitya (Death/Trial) | NADaseb |
Low activity | 1529 KN01 MDYK MUY | 29% (2/7) 33% (3/9) 67% (4/6) 17% (1/6) | 3.37 ± 0.66 6.19 ± 0.52 2.95 ± 0.26 2.97 ± 0.95 |
High activity | GT01 FI01 CR01 IYAT | 80% (12/15) 60% (6/10) 100% (12/12) 67% (4/6) | 57.03 ± 3.65 59.40 ± 4.76 114.30 ± 8.67 87.25 ± 5.22 |
No activityc | GT01Δnga SF370 | 0% (0/8) 17% (1/6) | 0.49 ± 0.13 -0.44 ± 0.80 |
Mouse infection model using nga knockout mutant and complemented strain
Virulence (Mortality) to mouse of GT01Δnga with or without cloned nga gene
Strain | Mortalitya | NADaseb |
GT01 (pLZ12-Km2, vector) | 73% (8/11) | 14.12 ± 1.30 |
GT01Δnga (pLZ12-Km2, vector) | 0% (0/17) | 0.04 ± 0.06 |
GT01Δnga (pLZN2, nga) | 0% (0/11) | 1.28 ± 0.12 |
GT01Δnga (pLZN-RBS, nga) | 0% (0/10) | 1.78 ± 0.03 |
GT01Δnga (pLZN-RBSII2, nga) | 29% (4/14) | 4.57 ± 0.17 |
Furthermore those results encouraged us to construct plasmids containing longer upstream DNA sequences than what is present in pLZN-RBS and pLZN-RBSII2. However these plasmids were not successfully constructed (data not shown, see Discussion in detail).
Assessment of body weight change in mouse infection model experiment
IFS-inhibition of the virulence of the GAS strain GT01
Recent reports have suggested that NADase is important for enabling GAS to cause invasive disease [13, 19]. These reports created our interest in NADase as a target molecule to reduce the GAS virulence. However, before studying the ability of a NADase-inhibitor to reduce GAS virulence, we felt NADase itself should be further characterized in its virulence causing role based on the following two reasons. (i) In M type-3 clinical isolate used in the previous study, the difference between mortality of mice infected with the nga strain and the parental strain was about only 25% [13]. Meanwhile, we recently reported that M-1 group A streptococcal isolates are divided into three groups based on NADase activity: high activity, low activity and no activity [15]. If a high-activity isolate is used to measure mortality of mice infected with GAS compared with the nga strain, the difference could be wider than if a low activity isolate were used. Indeed, in this study, we found that the difference between mortalities of mice infected with GT01 (12/15 = 80% death), a high-activity isolate, and the GT01Δnga (0/8 = 0% death) was 80% (see Table 2). In addition, the difference in mice mortality between the cases of GT01 (pLZ12-Km2, vector plasmid) and GT01Δnga (pLZ12-Km2) was 73% (see Table 3). This result shows that the GT01 isolate could provide an advantage compared with the M type-3 clinical isolate when studying the ability of a NADase-inhibitor to reduce GAS virulence, which is our original interest. (ii) To our knowledge, the reduced virulence of the nga-deletion mutant of GAS has never been successfully complemented using a cloned nga gene. It is common knowledge that complementation tests in vivo are not easily accomplished due to increased technical problems when compared to an in vitro study. In such cases, some alternative methods can be used. For example, Bricker et al. [13] constructed two independent nga-deficient mutants and showed that they have similar phenotypes. In this study, we added two more points of supportive data. We showed that an nga knockout GAS strain possessing a cloned nga gene partially restored virulence (Figure 2 and Table 3). In addition, we showed that a solution containing purified IFS suppressed the virulence of GAS in the experimental mouse-infection model (see later for additional discussion).
Although the data of Table 2 support our conclusion described earlier, some of the individual data were inconsistent with each other. For example, some of the strains belonging to low- and high-activity groups showed similar survival curves. However, this is not surprising because multiple factors play a role in GAS virulence, and the productions of virulent factors differ among the strains [25]. Therefore, it is important to compare groups including multiple, but not single, strains.
Regarding Nga knockout mutant and complementation, the NADase activity for the GT01 strain carrying the pLZ12-Km2 vector was different from that of GT01 alone (reported in Tables 2 and 3). Indeed, the virulence between the two strains also appears to be slightly different from each other, although we were unable to explain the reason.
Although the plasmid pLZN-RBSII2 conferred significant virulence to the nga strain when compared to a control vector (Table 3 and Figure 2), we found that the strain nga (pLZN-RBSII2) produced only 8% of the NADase activity found in the wild type strain. In order to restore NADase levels to near normal, we attempted to construct plasmids containing longer upstream DNA sequences than what is present in pLZN-RBS and pLZN-RBSII2. However these plasmids were not successfully constructed, possibly due to the potential toxicity of over produced NADase to bacterial cell.
As shown in Figure 4, injection of NADase inhibitor (His-IFS) significantly rescued mice from strains GT01. To further investigate the potential of the His-IFS solution, we tested strain CR01, which showed the highest virulence in the mouse-infection model among our collected strains (see Table 2). Although His-IFS alone was not sufficient to significantly rescue mice from the strain CR01, a combination of His-IFS solution and ampicillin was able to significantly decrease GAS virulence in mice compared with ampicillin alone (unpublished data). These results also show that NADase activity occurs in vivo and can be inhibited.
Using western blot analysis, we detected two bands from pHis-IFS using anti-RGS-HIS antibody (Figure 3). Based on the specificity of this antibody, we attributed the smaller band to degradation of the His-IFS protein.
The higher virulence of strain CR01 when compared to the other isolates belonging to high activity group (Table 2) may not only be due to higher level of NADase activity, but also due to additional unknown factors. For example, two-dimensional gel electrophoresis demonstrates that CR01 presents a different pattern of secreted extracellular proteins compared to the other isolates belonging to high activity group, including markedly lower level of the SpeB protein (unpublished results). Further analysis of the strain CR01, although the less representative strain among the high activity isolates had not been focused on very much in this study, would be a very interesting advance for the field.
Finally, we should discuss the discrepancy between NADase activity being important to the virulence of S. pyogenes during in vivo mouse models and our epidemiological data showing that low and high levels of NADase activity do not correlate with the severity of the S. pyogenes isolates in human infection. One possibility is that there is no statistical difference due to low sample number which is a result of a very small number of cases of the STSS disease. There is another possibility. After human passage, the isolated S. pyogenes could be different from the original strain which caused the infection due to getting genetic mutations. It is difficult to directly confirm the virulence of the passed isolates, since humans are the only natural hosts for the organism. When dealing with organisms, which lack a non-human natural host, we can never be perfectly certain and therefore must rely on additional accumulated supportive (usually indirect) evidence. If our purified His-IFS (NADase inhibitor) is able to rescue STSS patients in future that could provide a more ethically acceptable form of direct evidence.
We have presented further supportive evidence that NADase is important for severe invasive disease of S. pyogenes in vivo using the experimental mouse model. Furthermore, we provided useful evidence that the NADase is the potential target to suppress the virulence.
group A streptococci
immunity factor for Streptococcus pyogenes
- NADase (also Nga):
We thank Laura Leverton for critical reading of the manuscript and Hideki Matsui and Takayuki Ichikawa for technical assistance. This study was supported by Grant numbers 19590452 and 21790425 from the Ministry of Education, Science and Culture of the Japanese government. M. I. was supported by a grant for Research on Publicly Essential Drugs and Medical Devices, No.KHC1021 from the Japan Health Sciences Foundation.
Authors' Affiliations
- Cone LA, Woodard DR, Schlievert PM, Tomory GS: Clinical and bacteriologic observations of a toxic shock-like syndrome due to Streptococcus pyogenes. N Engl J Med. 1987, 317: 146-149. 10.1056/NEJM198707163170305.View ArticlePubMedGoogle Scholar
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- Schwartz B, Facklam RR, Breiman RF: Changing epidemiology of group A streptococcal infection in the USA. Lancet. 1990, 336: 1167-1171. 10.1016/0140-6736(90)92777-F.View ArticlePubMedGoogle Scholar
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- Sumby P, Porcella SF, Madrigal AG, Barbian KD, Virtaneva K, Ricklefs SM, Sturdevant DE, Graham MR, Vuopio-Varkila J, Hoe NP, Musser JM: Evolutionary origin and emergence of a highly successful clone of serotype M1 group a Streptococcus involved multiple horizontal gene transfer events. J Infect Dis. 2005, 192: 771-782. 10.1086/432514.View ArticlePubMedGoogle Scholar
- Michos A, Gryllos I, Hakansson A, Srivastava A, Kokkotou E, Wessels MR: Enhancement of streptolysin O activity and intrinsic cytotoxic effects of the group A streptococcal toxin, NAD-glycohydrolase. J Biol Chem. 2006, 281: 8216-8223. 10.1074/jbc.M511674200.View ArticlePubMedGoogle Scholar
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- Bricker AL, Cywes C, Ashbaugh CD, Wessels MR: NAD+-glycohydrolase acts as an intracellular toxin to enhance the extracellular survival of group A streptococci. Mol Microbiol. 2002, 44: 257-269. 10.1046/j.1365-2958.2002.02876.x.View ArticlePubMedGoogle Scholar
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- Bricker AL, Carey VJ, Wessels MR: Role of NADase in virulence in experimental invasive group A streptococcal infection. Infect Immun. 2005, 73: 6562-6566. 10.1128/IAI.73.10.6562-6566.2005.PubMed CentralView ArticlePubMedGoogle Scholar
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- Ferretti JJ, McShan WM, Ajdic D, Savic DJ, Savic G, Lyon K, Primeaux C, Sezate S, Suvorov AN, Kenton S, Lai HS, Lin SP, Qian Y, Jia HG, Najar FZ, Ren Q, Zhu H, Song L, White J, Yuan X, Clifton SW, Roe BA, McLaughlin R: Complete genome sequence of an M1 strain of Streptococcus pyogenes. Proc Natl Acad Sci USA. 2001, 98: 4658-4663. 10.1073/pnas.071559398.PubMed CentralView ArticlePubMedGoogle Scholar
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- Ashbaugh CD, Warren HB, Carey VJ, Wessels MR: Molecular analysis of the role of the group A streptococcal cysteine protease, hyaluronic acid capsule, and M protein in a murine model of human invasive soft-tissue infection. J Clin Invest. 1998, 102: 550-560. 10.1172/JCI3065.PubMed CentralView ArticlePubMedGoogle Scholar
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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July 7, 2014
The Centers for Medicare & Medicaid Services (CMS) posted the proposed 2015 Medicare Physician Fee Schedule (MPFS) [PDF] and associated rules for Medicare Part B services on July 3, 2014, and will publish it in the July 11, 2014, Federal Register. The proposal includes changes to reimbursement rates and the Physician Quality Reporting System (PQRS).
CMS continues to implement the rules established in 2014, which require private practice audiologists and speech-language pathologists to report the documentation of medications in the chart for 50% of the Medicare beneficiary patient visits in order to avoid the 2% penalty in 2017. However, CMS is recommending removal of the quality measure used by audiologists to report referrals to a physician for patients diagnosed with benign paroxysmal positional vertigo (BPPV) or dizziness. The CMS rationale for removal of this measure is that medical referral is a "common practice in order to provide effective treatment for patients."
For more information regarding PQRS, visit the ASHA PQRS webpage.
CMS will continue to administer the outpatient therapy cap exceptions process (using the KX modifier) and manual medical review at the $3,700 threshold for physical therapy and speech-language pathology services combined, which were extended through March 31, 2015, by the Protecting Access to Medicare Act of 2014, including for hospital outpatient departments.
More information on the therapy cap exceptions process and manual medical review is available on ASHA's billing and reimbursement webpages.
A conversion factor (CF) is used to calculate the MPFS reimbursement rates. The Protecting Access to Medicare Act of 2014 will prevent a drastic reduction in the CF between January 1, 2015, and March 31, 2015. CMS estimates that the CF for this period will be $35.7997, a reduction of less than 1% of the 2014 CF. However, CMS has projected a conversion factor for the remainder of 2015 that is 20.9% lower than the current conversion factor. Although this reduction is mandatory because of a statutory formula known as the sustainable growth rate (SGR), it is anticipated that Congress will enact legislation to prevent this reduction as it has done almost every year since the SGR was implemented.
ASHA will be submitting comments, due September 2, 2014, to CMS. The final rules for 2015 are expected to be published by November 2014.
Each year CMS releases proposed rules and rates for the following year with a 60-day comment period. Audiology and speech-language pathology services under Medicare Part B (outpatient) have reimbursement rates established by the MPFS. Speech-language pathology services provided in hospital outpatient settings are also based on the MPFS, while audiology outpatient hospital services are paid under the Hospital Outpatient Prospective Payment System. ASHA continues to work with CMS officials and will submit comments-on behalf of audiologists and speech-language pathologists-that reflect ASHA member concerns.
Please see the September 1, 2014, issue of The ASHA Leader for additional details. The current fee schedule and related information for audiologists and SLPs can be found on ASHA's Medicare Fee Schedule webpage.
For more information on the proposed rule, contact Lisa Satterfield, ASHA's director of health care regulatory advocacy, at [email protected] or Neela Swanson, ASHA's director of health care coding policy, at [email protected].
Feds Target Disparities in Special Education
Real-Time Text May Replace TTY
What Babies See May Be Able to Predict Their First Words
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Nice clarity.
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Recent research into climate change and plant growth indicates that both people and their pets may have a tougher time with allergies in the future.
Leaving aside the discussion about what is causing the warming of the Earth's climate, it seems that warmer temperatures and increased levels of CO2 in the atmosphere are helping plants grow faster.
This can mean more pollen in the air to stimulate allergies. My nose is telling me that the fall pollen season has started, and it may be worse this year than in the past.
Veterinarians see many pets with allergies, especially dogs, and the symptoms our pets have are a little different from the symptoms most people have. Where people who are exposed to allergens in the air have mostly upper respiratory symptoms (runny nose, sneezing, watery eyes, etc.), most allergies in dogs cause dermatologic signs.
The classic inhalant allergy pattern in dogs is inflammation of the skin inside the ears, in the armpit and groin areas, and on the feet. The owner notices more scratching and chewing at these areas. If the condition is not caught early and treated, the skin inflammation and self-trauma lead to secondary bacterial and yeast infections and hair loss, among other complications. An abnormal odor may be noticed due to the overgrowth of bacteria and yeasts on the skin surface.
Allergies can be treated, managed, and hopefully controlled, but they are rarely cured. There may be seasonal variations in symptoms for patients with pollen allergies, but when that special time of year comes around, we can expect symptoms to appear.
Antihistamines are a main line of defense against allergies. Antimicrobials, nutritional supplements, shampoos, other topical soothing agents, and, in really severe cases, steroids are used to manage our pets' allergies. Avoidance is great, but when we're talking about things in the air that we breathe, avoidance can be difficult.
If you notice an increase in scratching and chewing, increased body odor, or hair loss, your pet may have allergies. See your pet's doctor soon so that more severe secondary complications don't ensue.
(Dr. Greg McGrath is a veterinarian at Cedar Lake Pet Hospital in Biloxi, Miss. Questions for this column are encouraged. Write to South Mississippi Veterinary Medical Association, 20005 Pineville Road, Long Beach MS 39560 and include a self-addressed stamped envelope.)
(c) 2008, The Sun Herald (Biloxi, Miss.).
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Spears of Oblivion (3.5e Spell)
From D&D Wiki
Conjuration [Force] | |
Level: | Sorcerer/Wizard 9, Warmage 9 |
Components: | V, S, M |
Casting time: | 1 standard action |
Range: | Long (400 ft. + 40 ft./level) |
Area: | 25 ft. mobile emanation |
Duration: | Concentration (maximum 1 round/level) |
Saving Throw: | None |
Spell Resistance: | Yes |
Each person in the area affected by the spell is
immediately attacked by 1d3 spears of oblivion. The
spears use the caster's Base Attack Bonus to make ranged
touch attacks, and receive a competence bonus on each
attack roll equal to the caster's Intelligence modifier.
Targets immediately suffer 2d10 hit points of damage
for each spear that strikes them. On subsequent rounds,
any creature that spends any time in this spell's area of
effect is attacked by 1d3 spears and suffers damage, as
noted above, if hit.
Material Component: A miniature, gem-encrusted platinum spear worth at least 1,000 gp.
Back to Main Page → 3.5e Homebrew → Complex Special Ability Components → Spells → Sorcerer/Wizard


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What you need to know about the safety of the U.S. food supply, following the earthquake, tsunami and nuclear emergency in Japan
A message from David Schmidt, President and CEO of the International Food Information Council:
While some regions of California have experienced the effects of the tsunami, consumer concern about food contaminated by radiation from damaged nuclear power plants in Japan is unwarranted at this time. The level of radiation that could potentially reach the USA is anticipated to be very low and winds will dissipate it further.
The earthquake, tsunami and nuclear emergency were a devastating blow to the people of Japan. Our hearts go out to those who continue to suffer extreme hardships as these unfortunate events continue to evolve. What is occurring is very complicated and unprecedented. These conditions are being closely monitored and the experts' assessments are regularly relayed to us here in the United States.
We have learned that health authorities in the Asia region, including the Philippines, Singapore and India have begun testing any Japanese imported fresh produce for possible contamination by radioactive fallout. The European Union is taking similar action. Japan is not a large exporter of fresh fruits and vegetables.
In a March 17 public address, President Obama stated "We do not expect harmful levels of radiation to reach the West Coast, Hawaii, Alaska, or U.S. territories in the Pacific. That is the judgment of our Nuclear Regulatory Commission and many other experts. Furthermore the Centers for Disease Control and Prevention and public health experts do not recommend that people in the United States take precautionary measures beyond staying informed."
The World Health Organization has also cautioned people against self-medication with potassium iodide or products containing iodine, which they have stressed should only be taken when there is a clear recommendation from public health authorities, as in the case of an acute and direct nuclear emergency.
If you would like to know more about the risks of radiation please consult the following resources:
- U.S. Centers for Disease Control and Prevention Radiation Emergencies
- U.S. Department of State (advice for travelers)
- U.S. Department of Health and Human Services Response to 2011 Earthquake/Tsunami Disaster
- World Health Organization
- World Health Organization FAQ: Japan Nuclear Concerns
- White House Blog: "We Will Stand with the People of Japan"
Dr. Christine Bruhn, Director, Consumer Research, University of California Davis and Dr. Aurora Saulo, Extension Specialist in Food Technology, University of Hawaii contributed to the content of this article.


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Motivated at least in part by the current controvery over oneness heretic T.D. Jakes, (also here and here) please read two very good pieces on the importance of the Holy Trinity. Hopefully, this will help Christians understand that we are to separate with heretics, not dialogue with them.
Archive for the 'unitarian' Category
The Necessity Of The Holy Trinity
Posted by Job on October 26, 2011
Posted in anti - Christ, Apologetics, apostasy, Bible, blasphemy, blasphemy Holy Ghost, blasphemy Holy Spirit, christian broadcasting, Christian hypocrisy, Christian salvation, christian television, christian worldliness, Christianity, church hypocrisy, church scandal, church worldliness, corrupt televangelism, discernment, evangelical, evangelical christian, evangelism, false doctrine, false preacher, false preachers, false prophet, false religion, false teachers, false teaching, grace, interfaith dialogue, irresistible grace, Jesus Christ, Jesus Only, modalism, oneness pentecostal, oneness pentecostalism, orthodoxy, orthopraxy, Ruach Hakadosh, syncretism, TBN, TD Jakes, televangelism, trinity broadcasting network, unitarian, Y'shua Hamashiach, Y'shua Hamashiach Moshiach, Yeshua Hamashiach | Tagged: Baptist, elephant room, fundamentalism, God the Father, hermeneutics, Holy Ghost, Holy Spirit, holy trinity, monarchian modalism, Monarchianism, patripassianism, R. K. McGregor Wright, sabellianism, separation, trinity, Vern Sheridan Poythress | 4 Comments »
OK Oneness Liars Here Is The Best HISTORICAL Proof That Your Claims Are False
Posted by Job on October 25, 2007
Similar to Mormons and Jehovah's Witnesses, many Oneness Pentecostals claim that their unitarian view of the godhead is the true apostolic biblical faith and doctrine, and that the Holy Trinity came as a result of paganism being introduced into the church down the line. Well, if that is true, then this paganism took over the church rather quickly; long before Constantine and the Council of Nicea in the fourth century. Why? Because the oldest recorded Christian hymn, Hail, Gladdening Light!, which was being sung as early as 150 AD, is utterly Trinitarian! Now this date is key, because church tradition generally holds that the Gospel of John and Revelation were the last books of the New Testament and that one or the other was written as late as 96 AD, as the beloved disciple apostle John lived an exceptionally long time. So this hymn was being sung than 60 years after the New Testament was completed and the apostle John died, meaning that people that were personally taught by John and the other apostles were still alive, and thus would have contended against any paganism, heresy, or false doctrine expressed by it (as after all, in those days people actually did contend against false doctrines rather than just throw up their hands and say "who am I to judge … I am just going to remove the beams from my eyes before I go take the speck out of the eyes of the antinomian gnostics over there … who is to say that the montanists, marcionites, arianists, etc. are wrong and I am right … only God knows the heart and He will restore these good hearted Christians and forgive them on judgment day).
People, this is why the intellectual component of our faith, disciplines like systematic theology and church history, cannot be ignored. I would like to know what "oneness historian" deceiver Dr. Curtis Ward with his "unbroken Church lineage in which the oneness church began in the first century and has succeeded in continual perpetuity throughout history" says about this? Well, below is the hymn, and thanks to Ingrid Schlueter of Hope In Laodicea for providing it. Here post (which can be accessed here: Hail, Gladdening Light!) also provides an audio recording of the song.
Hail, gladdening Light, of his pure glory poured,
who is immortal Father, heavenly blest;
Holiest of Holies, Jesus Christ our Lord!
Now are we come to the sun's hour of rest;
the lights of evening round us shine,
we hymn the Father, Son and Holy Spirit divine.
Worthiest art thou at all times to be sung,
with undefiled tongue,
Son of our God, Giver of life, alone!
Therefore in all the world thy glories, Lord, they own.
Posted in abomination, blasphemy, Christianity, false doctrine, false teaching, heresy, Jesus Only, modalism, oneness pentecostal, oneness pentecostalism, unitarian | Tagged: church history, Hail Gladdening Light, historical evidence of trinity, hymn, trinity | 717 Comments »
Deepak Receives Healing And Spiritual Deliverance In Nepal
Posted by Job on August 22, 2007
Imagine if you saw a man living in the forest, a man who seemed to act more like a wild animal than a human. What would you do? You may be frightened and want to back away. For Gospel for Asia missionary Akash, the choice was simple: He would pray for the man. And as he and the believers in his GFA-affiliated church faithfully prayed for this man named Deepak, the Lord healed him! Read more about Deepak—and the other lives that have been touched in a village in Nepal—here.I appreciate you and your prayers, which make it possible for Akash to serve the Lord in Nepal. Yours for the unreached, K.P. Yohannan gfa.org
The sight of the man filled Gospel for Asia missionary Akash and his team with compassion. Left alone in the forest, this young man had been considered deranged for years. In fact, he scarcely seemed human. As the missionaries approached him, he muttered something incomprehensible. Once they heard him speak, they realized that he was demon-possessed.
Drawing near to Deepak, the missionaries began to pray for him. In the days that followed, they and the believers in Akash's congregation continued to pray for him. Within two weeks, Deepak was restored and delivered—much to the amazement of the other villagers. Everyone knew the kind of person Deepak had once been, and they were amazed to see his radical transformation.
Beginning to see that Jesus could heal anyone, the villagers brought to Pastor Akash six others who suffered from mental illness. As Akash and the believers in his church faithfully prayed for these people, the Lord healed each one.
The sight of the man filled GFA missionary Akash and his team with compassion.
Through the healings, people in the village are seeing God's power at work and His love for those living in bondage. Akash asks for prayer that all in this Nepali village will experience the redemptive love of Jesus in their hearts. Also pray for those who were healed, that they will grow in the love and grace of the Lord.
Links: Read more about Nepal and Gospel for Asia's ministry there. Why healings? Last 15 News Articles News Archives PhotoShows SEND! Magazine Video Archives
Posted in adultery, Asia, buddhism, christian television, Christianity, contemplative prayer, creationism, evangelism, faith healing, Hinduism, idolatry, India, Japan, missionary, multiculturalism, nepal, rapio, religious right, spiritual deliverance, spiritual warfare, unitarian | Leave a Comment »
Christian Missionaries In India Attending To People's Basic Needs
Posted by Job on August 17, 2007
Did you know that you can actually put warmth in a box? It's true! And many of our Gospel for Asia native missionaries need such a package right now, before winter sets in at Asia's higher altitudes. You see, when native missionaries serve in places like Nepal or northern India, they need to have warm clothing if they are going to reach the villages in the middle of winter. And while most have some form of winter clothing, it does wear out—and new missionaries join them each year.
Thank you for your help in reaching Asia for Christ!
Yours for the unreached, K.P. Yohannan http://gfa.org
Posted in Asia, Christianity, evangelism, idolatry, India, Japan, missionary, multiculturalism, nepal, religious right, unitarian | Leave a Comment »
Flooding Kills 2000 In India, Bangladesh and Nepal: Please Help!
Posted by Job on August 17, 2007
From http://gfa.org This is one of the rare times that I am saying that Gospel for Asia desperately needs your financial help quickly. But I feel that I must, and ask that you pray seriously about helping. This truly is an emergency situation.
As I wrote to you yesterday, more than 2,000 people have died in the record monsoon floods across eastern India, Bangladesh and Nepal. Literally millions have been left without homes, and their subsistence farms are under water in the worst flooding in 30 years.
Now, just this morning, I received a report from one particular area that brings this terrible situation closer to home. Our senior leader in Assam called to tell me that 4,000 homes in his area have been washed away. When he told me that over the phone, I was just silent for a moment. I could not imagine four thousand homes destroyed by floods in one place.
Then he made it even more personal–he said that 35 of our believers' homes among the Mising tribe have been been destroyed. Not only that, but 10 of our pastors and their families are also now homeless, and four of our churches have been washed away by the rampaging waters.
You can click this link to make an immediate gift. gfa.org/flood-relief-donation
We know the numbers in this disaster are going to increase, because many other believers have had their homes damaged, and some of our other 92 churches among the 3.5 million Mising people have suffered damage as well. So there will be reconstruction needs in the future.
But our immediate concern is for those who have lost everything–not only among our believers, but among all who are suffering because of the floods.
So far, 15 people among the Mising have died from the flood waters, and another 50 have died from the diseases that are now spreading because of the unsanitary conditions. This is a "mini-tsunami" in terms of suffering. And our response must be rapid.
That's why, in the midst of all this suffering, our GFA missionaries, pastors and believers are already ministering to those around them in the name of Jesus–bringing them what physical and emotional relief they can, while sharing with them the only true hope that can be found in times like these–Christ. But obviously they need our help, because they have little left to share themselves.
My friend, today we–and they–need your immediate help. You can make a gift to bring relief and hope to those who have lost everything by clicking this link: gfa.org/flood-relief-donation
Please understand, this report I am sharing with you today is just the first of many we will be receiving as the scope of this disaster continues to grow.
So I ask you today to help our GFA missionaries, pastors and believers who are at this moment caring for the suffering all around them with your gift that says you, too, want to reach out with love in the name of Jesus.
And what an opportunity! While 98 percent of the Mising are not Christians, now we can reach out, relieve their suffering and let them know that while their old life has been washed away in a flood, God is offering them a new life that will last forever.
Again, this link will take you to our site where you can make a difference in the lives of those who are suffering so terribly, including our Christian brothers and sisters among the Mising tribe. gfa.org/flood -relief-donation
Thank you for your love and compassion.
For the suffering in Asia,
K.P. Yohannan Founder and President Gospel for Asia
P.S. Click this link to read more about the monsoon floods in Asia. gfa.org/gfa-flood-relief-2007
Posted in Asia, bangladesh, Christianity, evangelism, false prophet, idolatry, India, Japan, missionary, multiculturalism, nepal, religious right, Ron Paul, unitarian | Leave a Comment »
Gospel For Asia Responds to Massive Floods In India, Nepal, and Bangladesh
Posted by Job on August 13, 2007
From Gospel For Asia founder K.P. Yohannan:
I am writing you today because I have just received word of some of the worst flooding in South Asia in 30 years—and our GFA native missionaries are already on the scene, ministering to the victims in the name of Jesus.
Simon John, one of our senior leaders in India, returned today from four days in Bangladesh, where he witnessed the floods first hand.
"It reminds me of the tsunami all over again in terms of the suffering," he told me over the phone.
And the floods not only are affecting Bangladesh, but India and Nepal as well. Please read the full story here, then please pray with me about how God can use us to bring relief to the victims. Any gift you can send will make a difference: gfa.org/flood-relief-donation
As you read this story, please pray for those who are suffering, and for our GFA Compassion Services volunteers who are ministering to them. Remember also that our missionaries and church members are among the victims of this disaster.
Thank you for standing with us in this difficult time.Yours for the lost in Asia, K.P. Yohannan
"This is some of the worst flooding I have ever heard about," said Dr. K.P. Yohannan, president and founder of Gospel for Asia, when he was told about the monsoon floods that have left millions homeless and 2,000 people dead in three South Asian nations."I grew up with monsoons every year," he said, "but this exceeds even last year's floods in terms of the number of victims—and we don't even have the final numbers yet."News reports indicate that about 2,000 people have lost their lives so far, and an estimated 28 million people have been driven from their homes. Bangladesh, Nepal, and the adjacent Indian states of Bihar, West Bengal and Assam have been especially hard hit.
In India's Bihar state alone, more than 12 million people have seen their homes and farmland submerged under the rising waters.
Now health experts are predicting an epidemic of waterborne diseases.
"It reminds me of the tsunami all over again in terms of the suffering of the people," Pastor Simon John told Dr. Yohannan by phone from Delhi. GFA's North India leader had just returned from four days in Bangladesh, working with the missionaries and church leaders there to coordinate GFA Compassion Services response to the disaster. He had been unable to communicate with the outside because of the damage to the communications infrastructure.
"Our people are involved in providing rice and beans, kerosene, cooking utensils and other items to the victims," Pastor John told Dr. Yohannan, "but transporting even these basics is difficult because roads are closed in many parts of Assam and Bangladesh. So our missionaries are walking or using bicycles, canoes or whatever other means are available to get to the victims."
"By God's grace we have the missionaries and believers at ground zero to mobilize very fast to help the hurting," Dr. Yohannan noted. "At the same time, many of our believers have lost their homes and are suffering as well."
Pastor John said that "in the beginning we didn't realize that it would be this serious. But the rains just didn't stop. . . ."
The North Indian leader also noted that many of GFA's church members make their living through menial jobs, living hand to mouth each day.
"In the floods, their jobs are gone," he said. "And that means no food-even if there was food available to buy.
"Yet regardless of their own suffering, "our people see this as an opportunity to minister to those around them in the name of Christ. Those that have not been as hard hit are giving of their own food, water, or whatever they can. And our churches are also raising what funds they can among themselves."
Of course, GFA's help goes far beyond immediate physical relief.
"This is a tremendous opportunity to show the compassion and love of Christ," Dr. Yohannan said. "I ask all Christians—whether in Asia or in the West—to join me in praying for the victims of this tragedy. And pray as well for our missionaries and our Compassion Services teams as they pray and counsel with the victims and strive to meet their spiritual and emotional needs as well as bring them physical relief.
"And as the Lord leads you, please help us as we help them. Any contribution you can sent will truly make a life-giving difference. We are committed to doing everything we can to bring relief and hope to these suffering people-not just for a few days, but for weeks and months to come." gfa.org/flood-relief-donation
Posted in Asia, Christianity, evangelism, idolatry, India, Japan, missionary, multiculturalism, nepal, religious right, unitarian | 1 Comment »
Final Answer: The Sinner's Prayer Is Not Only Unnecessary For Salvation, It May Not Be Expedient!
Posted by Job on July 5, 2007
I first asked the question here: Is The Salvation Prayer Necessary For Salvation?, which was in response to this challenge. Now I maintain that the reason why the issue of salvation is so vexing to the church is because there is no "instruction manual" in the New Testament, which I maintain is good because if there were, it would just be something else that Christians could use to turn into into a work or a ritual; claiming that the process in the Bible saved you rather than God. But a "type" of salvation by unearned grace is given in the Old Testament, in Isaiah 6:1-7. "In the year that king Uzziah died I saw also the LORD sitting upon a throne, high and lifted up, and his train filled the temple.Above it stood the seraphims: each one had six wings; with twain he covered his face, and with twain he covered his feet, and with twain he did fly. And one cried unto another, and said, Holy, holy, holy, is the LORD of hosts: the whole earth is full of his glory. And the posts of the door moved at the voice of him that cried, and the house was filled with smoke. Then said I, Woe is me! for I am undone; because I am a man of unclean lips, and I dwell in the midst of a people of unclean lips: for mine eyes have seen the King, the LORD of hosts. Then flew one of the seraphims unto me, having a live coal in his hand, which he had taken with the tongs from off the altar: And he laid it upon my mouth, and said, Lo, this hath touched thy lips; and thine iniquity is taken away, and thy sin purged."
All Isaiah did was realize and admit before God that A) God is Holy, B) he was sinful, C) that his sinful condition was unacceptable before God, and D) being unacceptable before God because of your sin is a bad thing indeed. (Please realize that Isaiah was ALREADY SERVING GOD IN THE OFFICE OF A PROPHET when he had this vision.) In short, Isaiah, acknowledged his sin and repented of it before God. Immediately upon doing so, his sin was removed through no act of his own!
But wait: his sin was removed by an angel, and only God can forgive sin! Not so! The angel was only the messenger, the vehicle, the vessel! Keep in mind: they WERE under the old covenant; Christ had not been born, died, and raised from the dead, and there was no gospel for a man to preach. The seraphim did not forgive Isaiah's sin, he took the live coal from the altar, put it on Isaiah's lips, and the sin was forgiven. Why was the coal live? It had been burned in the fire. What is a common metaphor for fire in the Bible? God the Holy Spirit; Ruach Hakadosh. Consider the Holy Spirit's role in salvation by reading this link http://www.eternallifeministries.org/awp_sal.htm, and while you are at it this brief opinion on salvation in the Old Testament in general here: http://www.biblebb.com/files/macqa/70-12-1.htm. And keep in mind: the fire of the Holy Spirit is not a regular, natural fire but a spiritual one. So, when the Bible says that the coal was "live", it does not merely mean that it was HOT, it means that the presence of the Holy Spirit was still with the coal. How do we know this? Because the angel used tongs! The angel never touched the coal, but had to use tongs to handle it. Why do you think that the angel would need tongs, brothers and sisters? This is an ANGEL we are talking about here, a spirit being! An angel is not going to be burned by handling the bratwursts from your barbecue grill! No, the angel could not – would not – touch the coal because the presence of God the Holy Spirit was upon that coal! And when the coal touched Isaiah's lips, the Holy Spirit that descended down from heaven by virtue of the coal carried by the angel (with tongs) played the same role in Isaiah's salvation as it plays in this dispensation when a man hears the gospel of a preacher and believes and trusts in the message through faith in God alone.
The best part of all this is that Isaiah played no role whatsoever in this. He did not choose the time or place or manner of salvation. He did not pray for his sin to be removed or for God to accept or help Him. And no, baptismal regenerationists, he was not baptized. He played no role in his salvation whatsoever. It was 100% initiated, executed, and completed by God. The only thing that Isaiah did was acknowledge that he was a sinner, and that he was unworthy to be accepted by God because of it! Immediately after doing that, GOD SAVED HIM. After all, what is the Name by which we are saved? Jesus (more accurately Yeshua, Y'shua, Yehoshua, or perhaps Joshua) which means "GOD SAVES."
So why, then, the gospel, and what of it? That would probably be a worthy topic for study, discussion, and debate, but I am satisfied with the explanation that it is the method that God told us to use and to leave it at that. But we see from the Isaiah experience that the critical role of the gospel is to not to get the sinner to ask God to save him. Why? Because Isaiah did no such thing! No, the critical role of the gospel is to get the unbeliever to acknowledge that he is a sinner and therefore unacceptable to God because of it, which is what Isaiah did! Only after the sinner realizes that he is a sinner and cannot be reconciled with God because of his sin can he believe that the purpose of the birth, life, death, and resurrection of the Jesus Christ Person of the Holy Trinity was to reconcile the sinner to God, and to trust that the actions of God alone are what saves him!
Again, I have no problem with a sinner's prayer, but only if it is said as a helpful exercise in "formally" fulfilling Romans 10:9 (That if thou shalt confess with thy mouth the Lord Jesus, and shalt believe in thine heart that God hath raised him from the dead, thou shalt be saved), specifically the "confess with thy mouth" part, only after the unbeliever has accepted that he is a sinner and even then only IF the sinner knows that the prayer does not save him and furthermore plays no role in his salvation! And this gets to be a real problem in my opinion. First, there is the well – known issue that a great many evangelists, especially those that rely on systematic methods (following a tract or a technique that they were taught during church evangelism training) soft – play the issue of sin because they do not want to alienate the unbeliever, and also because they may not be able to answer the unbeliever's questions concerning sin. They may mention it, but to get around that "problem", these evangelists purport to deal with the sin issue during the prayer, which usually starts off with "Lord, I admit that I am a sinner, please for give me for my sins." The logic "Hey, if he says it during a prayer that he is saying out of a sincere desire in his heart to be saved, then that counts as a confession of sin and God will accept it." No, it doesn't work that way. The person's KNOWLEDGE and SINCERITY is for God to save him, NOT of his sin and his separation from God because of it. This problem is added to when evangelists say that a person's separation from God is due to their not accepting Jesus Christ as their personal Savior. Well, did Isaiah accept Jesus Christ as his personal Savior? No.
The main goal for a lot of evangelists to get people to say a prayer that will not save them, even if they are sincere about a desire to be saved and sincerely recite the prayer! So how, then, are these people saved? I honestly believe that they are saved later. They go to church and (hopefully) hear preachers talk about sin and repentance (again, this cannot be presumed, even in evangelical and fundamentalist churches). Or they will (hopefully) read about the issue and (hopefully) understand when they (hopefully) read their Bibles (something else that cannot be presumed). What of praying for God to forgive your sins?1) Isaiah did not do this. 2) Nowhere in the Bible are we told to do this. 3) Will God hear the prayer of a sinner? Basically, the rub is that if you ask God to forgive your sins without meaning it or even having a true understanding of what you are asking, then it is a vain exercise. But according to the Isaiah example (and the other Bible salvation examples that I can recall), God cleanses you of your sin immediately upon your becoming aware of your sin, knowing that you are separated from God because of it, and desiring your situation to change. Thus, if you have heard and believed a true gospel presentation, then God has already forgiven you before even start to pray! (And it is a good thing too, since the prayer cannot save you!) And it may actually hinder you. What if you say the prayer (even if you mean it) without every truly dealing with the issue of your own sin? Then if you believe that your salvation came when you said your prayer, then will you even see how all of those sermons and Bible verses that speak of sin apply to you? If you regard yourself to be saved already by virtue of the prayer (or of baptism), then perhaps not.
So, the issue that must be dealt with is the scripturally untrue notion that the sinner's prayer plays any role in saving a sinner, especially when so many Christians are convinced that they were saved when or as a result of saying the prayer, and that the way to be used by God to bring others into the kingdom of heaven is to get others to say a prayer as they did. It is tempting to say that true gospel preaching would make this matter moot, but sadly it is not true. If you believe that God saves the person as a result of his saying the prayer, then out of your love for the unbeliever and burden for his lost soul, you are going to focus your entire energy on getting him to say the prayer. Everything else will be an exercise, a ritual, a formality. Which, of course, means that it will be EXPENDABLE; meaning that you can sacrifice some of it in order not to offend so that you might gain the person through prayer. Let us be honest: suppose you were to walk up to a person on the street and ask him "Do you want to be saved?" and the person replies "Sure, my best friend just died in a car accident, so I want to be with God when I die; I have heard people say and have seen it on bumper stickers and billboards that you need to accept Jesus to go to heaven, but I do not know how." The overwhelming majority of fundamentalist and evangelical Christians would leap for joy inside and say "Great, just say this prayer!" I would say that a sizable minority would give a quick gospel presentation about how Jesus Christ died for their sins – and may even briefly mention that the person is a sinner in the process – but that would be it! The person was sincere about wanting to be saved, the person said the prayer and asked God to forgive his sins, so there … he is saved! Before reading Isaiah 6 today, I would have agreed. But after reading it, I am forced to conclude that we cannot presume that the person is saved merely as a result of saying the prayer, and we can definitely know that the person's saying the prayer without knowledge of sin and his separation from God because of it and without repentance of sin because of that fact will not result in his salvation.
Illustrative example: Paula White! On Trinity Broadcasting Network about a year ago, Paula White was speaking of Without Walls' "extreme evangelism" methods. One such example was their giving a secular concert (admission was either free or with ticket prices far lower than normal) with R&B and rap artists to attract a large number of young unbelievers. That the concert was sponsored by a church was strictly withheld from the public, so the concert attendees had no idea that they were attending a church evangelism event and spent the entire evening there with nothing regarding Jesus Christ on their minds (or if they were, it had nothing to do with the event that they were attending). After the last act performed (and again, it was a secular concert playing secular music, not that I am condemning secular music or those who make or listen to it, just to point out that Christ or the gospel was unmentioned throughout the entire event) an announcer came on stage and asked if anyone in the building "wanted to accept Jesus Christ in their lives" (Paula White's words on TBN, not mine). Of course, when you have a bunch of impressionable young people that have spent several hours being entertained by their favorite musicians in a concert setting (and R&B and rap acts frequently ask their audiences to respond … "Get up and say yeah!" sort of thing), they are going to be willing to answer in the affirmative to virtually anything almost without thinking. So, when a bunch of kids did so, the announcer had them stand up and say the sinner's prayer! Which meant that if they were sincere in saying the prayer (and when you are a kid with your friends at a music concert, you can be sincere about a lot of things, especially for oh, about 30 seconds that it takes to say such a prayer), then they were saved. Right?
One final thing: Isaiah 6:8 says "Also I heard the voice of the Lord, saying, Whom shall I send, and who shall go for us? Then said I, Here am I; send me. send, and who will go for " Once again, you have God referring to Himself as I (singular) and us (plural). I = Unity, us = Trinity. Trinity in Unity, or Tri – Unity.And in this context, this whole "royal we" (where dignitaries refer to themselves in plural) not apply. Else, the verse would have read "Whom shall WE send, and who will go for US?" No way the normal language would have been used for "I" and the royal language for "we." Yet another example of why there is no excuse whatsoever for calling yourself a Christian while denying Trinity, or making the "the true nature of God is an unknown mystery that has not been revealed" or "the word Trinity is never given in the Bible" canards that are commonly used as excuses for following some Jesus Only oneness preacher or movement. And isn't it amazing that God JUST HAPPENS to declare His Tri – Unity during this salvation scene? Do not be deceived, it was no coincidence. God referred to Himself as "I" and "We" during that vision precisely because the Tri – Une nature of God is integral to salvation, and that there is no salvation apart from it. All oneness dissenters: please explain how a "manifestation" of God or a "father – son – uncle relationship of God" went with that live coal to burn the sin out of Isaiah!
Posted in baptismal regeneration, charismatic, christian broadcasting, christian television, Christianity, false doctrine, feminism, GOP, grace, Jesus Only, occult, oneness pentecostal, oneness pentecostalism, salvation, salvation prayer, sex crime, somalia, spiritual warfare, TBN, the three steps to salvation, trinity broadcasting network, unitarian, Y'shua Hamashiach Moshiach | 11 Comments »
From The Gospel For Asian Mission Field: Goat Milk for the Soul
Posted by Job on July 3, 2007
In his village on the border of Nepal and India, Arnash struggles to provide for his family's survival. But every Sunday, they come to the GFA-affiliated church to worship Jesus, who sustains them. And the rest of the week, they find joy in Him even while they labor to earn enough to eat. Read the story of how God provided for Arnash through a friend of Gospel for Asia.Please remember people like Arnash in your prayers. God is doing great things! Thank you for your partnership.
Yours for the unreached, K.P. Yohannan Founder & President
Posted in Christianity, evangelism, idolatry, India, missionary, multiculturalism, nepal, religious right, unitarian | Leave a Comment »
Carlton Pearson Defecting To The Religious Left: Not As Big A Deal As It Seems
Posted by Job on June 27, 2007
Here is the MSNBC.com link To hell and back – Dateline NBC – MSNBC.com on Carlton Pearson, the former Pentecostal megachurch preacher out of Tulsa who has joined up with the Episcopalians, with the gay rights issue being the main motivating factor. Now some might say "what a tragedy for a preacher to turn his back on the Bible like that." I say instead that all Pearson did was add to the false doctrine that he was already preaching, and that his new doctrine was no worse than what he was deceiving the nations with before. How so? Because Carlton Pearson was already preaching that godliness is gain (see
The Prosperity And Word Of Faith Blasphemers) through his Word of Faith/prosperity doctrines. You know, the same group of people that refuse to explain the difference between the promises given to natural Israel under the Old Covenant and spiritual Israel under the New Covenant in order to not only cause people to think that God is some genie in a lamp that exists to grant their every wish; that God has given them the right to stand in His Place and use His Name to be "little gods" (to create and destroy with the power of the tongue, so that our will shall be done on earth and in Heaven rather than God's); but also that God loves them so much more than He loves Christians suffering in Africa, Asia, the Middle East and all of these other places … that He saw fit to give us alone all of this peace and prosperity! If only those Third World Christians had our faith; if only they had our "favor from the Lord"… if only they tithed like we did, then their "overflow" would come, right?
And that symbolizes the problem with a lot of Christians today. So long as Carlton Pearson was merely being a WORD OF FAITH/PROSPERITY blaspheming heretic directly contradicting scripture and being given over to a reprobate mind (again, read The Prosperity And Word Of Faith Blasphemers to see what I am talking about) … so long as he kept his transgressions in the spiritual realm where people can't see, then he was fine in the eyes of so many good decent Christian folk. But oh, the minute that he stepped into the natural realm, the minute that he began to transgress "traditional Christian values" by extending his apostasy to – gasp! – HOMOSEXUALS, then that is when his members got up and left and they had to padlock that megachurch empire of his built on false doctrine. And that is what is so silly about this whole thing. We Christians really do have to grow up with respect to this homosexuality thing, OK? I mean come on, THEY ARE JUST PEOPLE! Homosexuality is nothing else but another sin that God will forgive you for. No different from gluttony, gossiping, lying, adultery, etc. Look, if you are in a lifestyle of gossiping and claiming that it isn't a sin and that you are so righteous and sanctified and holy and speak in tongues, God is going to burn you for eternity in the lake of fire while that homosexual (see Demon Of The Day: Homophobia) who cries out to God in repentance and salvation will be accepted just fine in heaven! You know what Yeshua, the Hebrew name of Jesus Christ means? God saves. God saves homosexuals. God saves those that have had abortions. God saves thieves. God saves murderers, rapists, prostitutes, and drug addicts. God saves people from other religions. God saves all who repent of their sins and seek after His Truth. But you know who God will not save? Someone who has His Truth in his hands and REJECTS IT to go after LIES, after devil's doctrines! So, before you give some "profile in courage award" to people who left Pearson's church when he started preaching his "gospel of inclusion", you need to ask them A) why were you in that church to begin with and B) did you just leave him to take up with someone else who hates and rejects the true gospel and denies the true nature of God? So no, the tragedy with Pearson is not that he has started preaching inclusion for homosexuals. The tragedy is that A) he was preaching false doctrine long before that, B) people were following him then and C) would still be following him now had he limited his false doctrines to godliness being gain. And that is something that all of us Christians need to admit to ourselves and start seeking the Lord in prayer about; contending earnestly and fighting the good fight of faith for our brothers and sisters in Christ that are in real spiritual jeopardy because they are (knowingly or not) following after lies.
Posted in Apologetics, apostasy, blasphemy, CAIR, christian broadcasting, conservatism, contemplative prayer, false doctrine, false preacher, false preachers, false prophet, false religion, false teachers, false teaching, full gospel baptist, George Bush, global warming, GOP, heresy, homophobia, homosexuality, innocent blood, Jehovah's witnesses, marriage, oneness pentecostal, oneness pentecostalism, political correctness, post abortion syndrome, prosperity doctrine, Republican, sex crime, sexual exploitation, somalia, spiritual deliverance techniques, spiritual warfare, syncretism, terrorism, Tim LaHaye, unitarian, Word of Faith | Leave a Comment »
From Gospel For Asia Ministry: God Provides Food For Poor Nepal Family
Posted by Job on June 12, 2007
Balji did his best to provide for his family, but in his destitute village in Nepal, it was almost impossible. Thankfully, Balji and his family were Christians and trusted the Lord to provide. And He did, but not in a form they expected. Read Balji's story here gfa.org/hope-on-a-leash?motiv=WA76-G1PS.Many families like Balji's are being impacted through your prayers and gifts. Thank you for your partnership to see Asia come to Christ!Yours for the unreached, K.P. Yohannan Founder & President gfa.org/pray?motiv=061207
Posted in Asia, evangelism, idolatry, Japan, missionary, multiculturalism, nepal, religious right, unitarian | Leave a Comment »


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From Your Sink Since 1900
by jewelwing
Printed to order
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Ships in 1-2 business days.
Hundreds of years ago, people would get up in the morning and walk to the center of town, where they would lower their buckets into the well and bring up water so that they could drink. Today, we get up in the morning and ride our bikes to the corner store where we can take bottles from the freezers so that we can drink. And also we pay for it. We've come so far!
But our water supply has so much to offer. All those drugs, flushed down the toilet. All those hormones from birth control and mood altering medications. Tiny shellfish and the occasional bit of metal from pipes our grandfathers made. You know what that makes a person? Strong.
So bring it on, aliens! We don't need those cold germs this time. We've been drinking cow hormones for a couple of decades now! We're a country of angry minotaurs, and we're ready! Mooo! Moooooooo!
This shirt was designed by: jewelwing. who looks lovely but is probably too heavy to fly. Diamonds aren't real aerodynamic, peacock.
Wear this shirt: when you're going for a drink with Gregory Hines.
Don't wear this shirt: if you're a Dasani rep. There's no need to give away all your trade secrets.
This shirt tells the world: "You know, for the same price we could just have a few beers…"
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Agile Testing
Did anybody say webscale?
Thursday, December 22, 2011
Load balancing and SSL in EC2
Here is another
I wrote for this year's
blog. It briefly mentions some ways you can do load balancing in EC2, and focuses on how to upload SSL certificates to an Elastic Load Balancer using command-line tools. Any comments appreciated!
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All Posts Tagged Tag: 'soundcloud'
Video on YouTube, Streaming Music on SoundCloud, Photos on Flickr
Yes, we're on YouTube, SoundCloud and Flickr now. Going forward we're going to make lots of new material available for you to enjoy on a regular basis. Hey, it's Baroque, but it's also the 21st century.