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In a world where we can now speak out instantaneously about the latest news, it might be wise to consider a few things before posting.
I try to write my blog posts in an informative and unbiased way. Sometimes that's harder to do than I anticipate. But I believe by pointing fingers and playing the blame game, we accomplish little and antagonize a lot. My goal is to try to reach out to the general public and offer useful, thought-provoking material that anyone can relate to. Sometimes I'm providing factual information, and sometimes I'm attempting to look at issues in a more philosophical manner, Today I want to do a little of both.
On a daily basis, we see cartoons, hear jokes, or read articles that are disparaging, derogatory or distasteful. Most of these are directed at political figures or groups, or at sports or entertainment celebrities. But some are against ordinary groups of people, like 'all republicans' or 'all democrats' or in the worst cases, against an ordinary individual. On occasion, the joke or cartoon is not meant to hurt anyone, but is a thoughtless depiction derived of a dark sense of humor. When aimed at an individual, it becomes a type of bullying and/or discrimination. This is the kind of stuff that we read about, where a teenager commits suicide after being shamed or humiliated online or at school. What started out as a bad joke can turn into an insulting, hurtful continuum of degrading ridicule, leading to utter despair by the targeted person.
Kids are generally not mean spirited and cruel by nature. Most of the time this is learned behavior – sometimes by the way they're treated, and sometimes by watching the behavior of others around them. At times, they get swept up by their peers and join in tormenting others in order to be accepted by a desired social circle. However, more often than not, they learn this type of disrespect from their home environment, whether it be from parents, siblings, or other relatives. For more insight into what might lead to bullying behavior in your child, https://www.healthyplace.com/parenting/the-parent-coach/causes-of-bullying-bullying-behavior-in-bullying-child/ leads to a site that offers a more in-depth explanation of how a child can become a bully, even when they aren't actually being exposed to direct bullying tactics. One quote from this article jumped out at me because, as a parent, I know this to be true: "Just because a child might not always "listen" to our requests and instructions doesn't mean they aren't intently listening to our views of other kids, parents, teachers, neighbors, and so on."
I would like to focus on the ways in which we unintentionally – or intentionally – bully and disrespect other people in our daily interactions. At work there is usually a code of behavior that's implemented in the work environment. Human Resource Departments spend large quantities of their time arbitrating disputes and harassment claims between employees. Granted, some companies are better at doing this than others. At least there are rules.
But how do we interact with others in our "free time" or social activities? When approaching an opinion other than our own, do we let fly with the first thought that comes to mind, or do we consider the effectiveness of our words? There is a well-known saying, "Better to be silent and thought a fool than to speak and remove all doubt." Of course, we've all said something at times that we wish we could take back. Anger generates an even more favorable climate for regrettable moments. Perhaps politics is the most conducive element for destructive thoughts, words and behavior. For one thing, we all think we're right when it comes to politics. Of course that's not possible, but who wants to go first and say, 'maybe I was wrong'? Who's going to even think it?
What's shocking, though, is the amount of vitriol we see in social media. I often wonder, do these people talk this way to everyone, or is it the anonymity of the Internet that removes their inhibitions? I'm not going to give you any pictorial examples of the things I see posted on Face Book, Twitter, or other social media sites because if I did, most likely my site would be shut down for pornographic or inflammatory content. The verbal abuse is bad enough.
I'm not speaking of trolls, or paid antagonists, or juvenile personalities that jump onto their parent's accounts. I'm talking about supposedly reasonable adults, maybe even relatives or people you know, that curse and insult not only a targeted politician like Donald Trump, Bernie Sanders or Hillary Clinton, but belittle anyone who supports them, or anyone who questions a political stance. Hillary Clinton used a poor choice of words when she called Trump supporters "a basket of deplorables." I believe she was referring to the KKK which had endorsed Trump for president, or possibly the White Nationalists/Supremists who also backed him. Unfortunately, she unwittingly encompassed all Trump supporters with her statement. It sets up an atmosphere where it's okay to use labels like repuklicans or demoRats. I suppose these names are considered to be clever. To me, the users seem ignorant. It's as if they don't have enough grasp of the English language to describe their thoughts and feelings, so they resort to name-calling of the basest sort.
Most people have also seen the vicious cartoons about the Obamas, either dressed in Muslim garb, or depicted as apes, and the one of Trump tweeting while on the toilet, or in an inappropriate embrace with Vladimir Putin. There are even pictures of a naked Trump statue that someone wasted their talents on. What kind of mentality finds this humorous? It's true that the shock of seeing some of these elicits a reflexive or embarrassed laugh, but if this is a sign of a person's true sense of humor, I don't think I'd want to go see a comedy with them. In any event, I personally find these pictures offensive. I might agree with the sentiment, but I think less of the person who addresses it in this manner.
My parents brought me up to understand that hurting someone's feelings is cruel. To attack their weaknesses or physical differences was shameful. To call someone fat, or ugly, or stupid, etc. shows that you are insensitive to others' feelings. Furthermore, I can't think of a single instance where this name-calling helped a situation. The same goes for cursing. I know all the words and what they mean, (and I've even used some in a fit of anger.) I know what STFU means, too, and GTFO. Google them, if you don't. I was also taught that cursing shows a lack in vocabulary. That's not necessarily always the case, though. Frustration often lends itself to cursing, but it's still not a good way to communicate.
The question here is why are we arguing with people that we disagree with by going down to a level of hate-filled obnoxious commentary? When someone tells you to STFU, does their point of view even matter to you anymore? So if you talk to them that way, is there any reason to wonder why they don't get your point? And the hate is so penetrating. In group discussions, I have seen people type that they no longer speak to members of their own family because of a political stance. They say they will never even knowingly talk to someone from the other political party. Why? How can you learn if you cut yourself off from understanding a different perspective? No one says you have to agree, but wouldn't it be better if you consider their side, even if only to give yourself a better way to argue once you understand where their thoughts come from?
Here are some random comments I've seen on group postings and article comments. They come from both liberal and conservative sites and you would be wrong if you tried to guess:
- A**hole
- Drug addict
- You are a nitwit (unable to determine who)
- —shut places down so the Chinese and Russians can't dump their trashy kids there…
- F'ing morons
- Facebook, please … add a puke emoticom
- Godzilla outshines Moo-shelle
- I can understand why leftist women are so angry. All you have to do is look at them, if you know what I mean.
- Who cares if it wipes out Mexico? LOL (re: Hurricane Katia)
- You pray and God preys
I'm going to throw two other points in here. One is criticism of a person's religion (unless, of course, the subject is religion to begin with). The other is making false statements. These are two methods of arguing that are cheap shots and have no place in social discourse. False statements not only detract from your argument, but if believed can lead to serious mistakes and sometimes physical harm to other people – not to mention terrible election outcomes from time to time.
The best way to critique an article is by presenting facts and where you obtained them. Sometimes that might be personal experience, which is fine. Just use acceptable language and aim for good spelling and punctuation.
When replying to a meme, remember that lots of people you don't know might be affected by what you say. You don't have to sound like an English professor, and you may certainly offer criticism. That's part of what makes these sites like Twitter, Instagram and Face Book so popular. Just be kind, or express your frustration or anger in a non-combative way. If you would be offended should someone say something similar to you, don't say what you were about to say. – END


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IQ Genex is versatile. I suggest all executives to do so. That isn't a moment of glory for IQ Genex. You need to choose, among other things, the right IQ Genex for you and this is an ironclad statement. I was going to try a different IQ Genex. No matter how you examine that, you must be cautious. Maybe I may not be partly wrong referring to this. I don't have to paint myself into a corner. That's the time to take a load off while so much for coming in last place. That grew out of the frustration I've heard from competent people. They gave me carte blanche to discuss IQ Genex. You have to ignore this: I am a recognized guru in IQ Genex. I'm searching for game plans to correct for this by the time mentioned. That is according to the instructions I have read in relation to IQ Genex because you won't locate rivals for the job. That is IQ Genex unleashed. The key is keeping a lid on IQ Genex.
I immediately began giving IQ Genex another look. They were on edge. You ought to lay your cards on the table.
I am somewhat new to writing in connection with IQ Genex and who has been paying attention to this. I ought to say it I have to take into account that stock flash in the pan. The standard modern IQ Genex contains this, which will help with IQ Genex. What could you do with a IQ Genex?
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Analysis of the global T m–T s correlation and establishment of the latitude-related linear model
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- Citations
In this study, the correlation between T m, a key variable in GNSS water vapor inversion, and surface temperature (T s) was calculated on a global scale based on the global geodetic observing system (GGOS) atmosphere T m data and European centre for medium-range weather forecasts (ECMWF) surface temperature data. The results show that their correlation is mainly affected by latitudes, and the correlation is stronger at high latitudes and weaker at low latitudes. Although the correlation is relatively weak in the tropic areas, the temperature changes so little in a year in these areas that we can still achieve good T m results by linear regression model. Based on these facts, "GGOS atmosphere" T m data and ECMWF T s data from 2005 to 2011 were used to establish the global latitude-related linear regression model. The new model has root mean square error (RMSE) of 3.2, 3.3, and 4.4 K, respectively, compared with respect to the "GGOS atmosphere" data, COSMIC data, and radiosonde data and is more accurate than the Bevis T m–T s relationship.
KeywordsCorrelation Linear regression Zenith wet delay Water vapor GPS
Thank the ECMWF for providing surface temperature data and "GGOS atmosphere" for providing global T m data.
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- 11.Liou YA, Teng YT, Van Hove T et al (2001) Comparison of precipitable water observations in the near tropics by GPS, microwave radiometer, and radiosondes. J Appl Meteorol 40:5–15CrossRefGoogle Scholar
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- 14.Wang Y, Liu LT, Xu HZ et al (2007) Retrieving change of precipitable water vapor in Chinese Mainland by GPS technique. Geom Inf Sci Wuhan Univ 32:152–154Google Scholar
- 15.Jade S, Vijayan MSM (2008) GPS-based atmospheric precipitable water vapor estimation using meteorological parameters interpolated from NCEP global reanalysis data. J Geophys Res 113:1–12Google Scholar
- 16.Yao YB, Zhu S, Yue SQ (2012) A globally applicable, season-specific model for estimating the weighted mean temperature of the atmosphere. J Geod 86:1125–1135CrossRefGoogle Scholar


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For those unaware, you've probably heard Zach Sobiech on the radio. He created a hit song called "Clouds", but what is far more touching than his song is his story. Many of us are all worried about what we'll do in 20 years, but what would you do if you only had 4?
For those interested, here's a second documentary showing his family one year later. It's definitely sad watching them cry and talk about him, but it's also very moving to see how they've created such meaning from his passing.
At times I find it hard to watch videos that are so sad and emotional, but I think it's important to get those reminders. You can worry about your job, you can worry about your future, but make sure you always cherish now and love the people around you. You never know when you will get another chance.


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Two men who were arrested after shots were fired Dec. 26 in celebration of the Washington Redskins victory over the San Francisco 49ers were granted probation before judgment Tuesday in Washington County District Court.
Robert W. Socks, 22, of 834 Spruce St., Hagerstown, and Mitchell Mason, 19, of 63 Broadway Apt. 6, Hagerstown both had been charged with reckless endangerment, discharging a firearm within city limits and hindering a police officer.
Socks pleaded guilty Tuesday to discharging a firearm and was fined $325 by Judge Ralph France. His weapons were forfeited for the one-year period of his probation.
"I know what I did was really stupid ... it won't happen again," Socks said.
Mason, who Hagerstown City Police found in Socks' attic, pleaded guilty to hindering a police officer.
Police responded to Socks' home just before midnight after getting calls from neighbors about shots being fired off the back porch.


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Outgoing Majority Chief Whip, Mubarak Mohammed Muntaka says Parliament would be unable to pass the Right to Information Bill (RTI), less than 24 hours to the dissolution of the House.
He said discussions between New Patriotic Party (NPP) and National Democratic Congress (NDC) Members of Parliament (MP) over the passage of the bill were not positive.
Speaking to Evans Mensah, host of Joy FM's Top Story programme Thursday, the Asawase Constituency MP was doubtful if the bill would see the light of day before midnight of January 6.
Outgoing President John Mahama has charged MPs to build consensus to ensure the passage of the RTI bill when he delivered his final State of the Nation Address.
He thanked the House for passing the many bills his government has brought before it, saying Ghanaians would be proud if the longest bill in the history of the country is passed into law.
But Mohammed Muntaka who is the Minority Chief Whip designate for the seventh Parliament said it would be difficult for the outgoing President's wish to be honored because of the lack of consensus in the House.
"I doubt with all sincerity and apologise that we will not be able to do the wish of the outgoing President," he said.
Ranking Member of Parliament's Legal and Constitutional Affairs Committee, Joe Osei Owusu described the outgoing President's wish as laughable.
He said President Mahama's call for the passage of the RTI bill was coming at the wrong time after he had failed to push for the passage of the bill during the eight years of the NDC government.
"It is a wish, but didn't come in good time to be respected."
Source: Myjoyonline


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Great White™ magnetic whiteboards are Optima's best porcelain steel whiteboard and a New England tradition. Each board carries a lifetime warranty on the dry erase writing surface and each unit is handcrafted on our factory floor where we control the quality from start to finish. These are among the finest magnetic whiteboards you will ever find and we stand behind each unit that we make.
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Preparing for national qualifiers
In all four years that I competed in high school, nothing to me was quite as daunting as the national qualifier each spring. (Depending on your state, this can range anywhere from february to april.) After a long year of competing and revising both your piece and performance, you finally reach the breaking point. Win or go home. It's that simple and this brings staggering pressure to the say the least. I have seen a couple big victories, but have also fallen just short as well. Either way, there are some things that you must keep in mind as you approach this pivotal tournament.
There are a few rules that any competitor must be aware of before the national qualifier. Regardless of state or district, the NFL mandates that only the top 2 competitors can qualify in a given speech event unless there are at least 38 competitors in an event. In most districts, there wont be 38 competitors in any event; therefore only 2 qualify. Although this seems very doable, view it in a different light...You are only allowed to have ONE competitor beat you if you plan to go to nationals. Unless you are from a pathetically easy district, this is a very formidable task.
There is another rule that most competitors don't understand because it is different from all other tournaments. The NFL has a rule for national qualifiers called "2 downs and you are out." In order to understand this, let me give an example. Let's say your speech category has 30 competitors. You will have 5 rooms of 6 competitors. An "up" is considered positions 1-3 and a "down" is considered positions 4-6. Therefore, if you get two downs, you are out of the tournament. Although this is tough enough, especially if you have a stacked room, it gets even tougher. After the drops before round 3, they move to accelerated drops meaning "ups" are only placements of first or second place. Not only is this tougher, the competitors are tougher because the weaker opponents have already been dropped.
These are the two major rules that competitors must understand. You have to be familiar with your surroudings and the procedures if you are to succeed. With these two rules comes one last piece of advice: Get lots of sleep the night before. I can tell you from much experience that endurance (on top of a great piece of course) is the key to qualifying to nationals. I remember my sophomore year specifically. I was competing in oratory and there were 41 competitors. Based on the above-stated rule, the top 3 were guarenteed to go to nationals. Competition started at 9 AM and by the time a final round was determined, it was 8 PM that night. It had already been 5 rounds and now the 6th and final round would determine who went to nationals. I can honestly say that I have never been more fatigued for a round than I was for that one. I dont care how many years you have competed or how much you have prepared, it takes A LOT of endurance to stand strong after that much competition.
These are just a few hints. Obviously, there is much more that goes into perfecting a piece that will get you to nationals. But just remember this. Each year, a little more than 2% of competitors make it to nationals. That is extremely difficult; therfore the margin for error is slim. But if you are ready for the tournament and understand the rules, you put yourself a leg up on the competition.
As a person who never even had the option of going to Nationals, and who was never awesome enough to break to State, I found this all very interesting. I got exhausted just reading it. I couldn't imagine competing in it. I bet it's fantastic fun! Load up on Redbull...haha, just kidding. The crash would be horrific!
Question...it seems "very doable" being the top two competitors to qualify? Who is saying that?! I would like to add a third thing to the list of preparedness for going to Nationals: do not go in expecting to qualify; like you already have it. Go in prepared to fight for it. Be a Spartan! I am fairly sure anyone who thinks it's easy to be in the top two, and that they own it, will not have prepared properly and fail. Just saying.
Kudos to anyone who gets to Nationals at all. That takes some mad skills.
This is a great breakdown. And yes, definitely get plenty of sleep before a national qualifier! Staying up late the night before a tournament can be fun, but a qualifying tournament is not the best time to wake up with bags under your eyes and a desperate need for coffee...


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As the new mini season of The X-Files is fast approaching, I've been wanting to do a rewatch but I've also been wondering how to do it. Luckily, Fox has done the timing for me, and is having an unofficial rewatch. They aren't airing these episodes (they want you to buy the DVDs or do Netflix), but it is a nice idea to get fans back into the thing.
As I've mentioned, I've been wanting to do a rewatch and possible review series for a good long while now, but now seems as good a time as any to do so.
Reviewing a pilot is an interesting thing. There are so many considerations that go into making a pilot that it's hard to treat it as a normal episode of television. It's made to entice TV executives to order more episodes, but it's also made to get TV watchers to keep watching the show. It's supposed to be the start of the story but also is supposed to show what a typical episode might look like. Pilots are also notoriously shaky – some of my favorite TV shows have pilots that I would flat out tell people to skip, or insist "no it gets better, believe me.
With all that being said, The X-Files actually has a fairly remarkably good pilot. While it's not a perfect episode (I think the "this is based off of true events" angle is a bit hokey), and there are certainly much better episodes throughout the series run, it does a pretty fantastic job of introducing the basic premise of the show and getting across the general feel of the show. The firs meeting of Mulder and Scully, for example, has dialogue where he's explaining why he's a believer and why she's a skeptic, and it perfectly conveys what their interactions are going to be.
The scenes featuring just Mulder and Scully, in particular their first meeting and when they bond in his hotel room, are the strongest scenes of the episodes. The chemistry between the two of them is already there in full force, which makes their partnership relationship wonderful to watch. Honestly, their relationship works almost TOO well right away; it feels like they are already trusting each other from the getgo, which doesn't quite work with the narrative.
The rest of the plot is pretty typical X-Files, but again goes a long way in establishing the tone of the show. Possible abductions! Lost time! Possible conspiracy! While the merit of the entire mythology arc has been debated a lot (I personally like them but I understand that there are serious flaws with the mytharc), this episode does a fantastic job of getting to the core of the mytharc and Mulder's journey.
It's also almost remarkable how the visual tone of the series is established. This show still looks great, and in part that's because the cinematography (in particular the lighting) still looks wonderful. When I took a film production class in college, The X-Files was used as an example of effective lighting, and that's not wrong. The use of shadows and chiaroscuro lighting gives The X-Files some unique visuals, and it looks great.
The acting is pretty solid for the most part. Gillian Anderson is the always wonderful and David Duchovny manages to hold his own with her. Some of the guest actors aren't too great (Theresa Nemman's actress is a little shaky) but, for being a TV show in the early 90s (before film actors and production started to migrate to TV) the acting is solid.
Ultimately this episode (and most of The X-Files) holds up remarkably well for being over 20 years old. The strong writing, characters and cinematography make it work really well. There are a few elements that feel dated (certainly the technology they use is quite dated), but as a show this holds up. I first became an X-Files fan in late 2005 – early 2006, and the show worked well on first watch then, but it still works now.
While there are definitely better, more ambitious episode of The X-Files, this is just about a perfect pilot episode and a great introduction to a great series. 8/10.


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Another festive picture for you this week from December 1992.
This time we are featuring youngsters from Hipsburn First School, who were making Christmas cards to send to friends and family.
Do you recognise any of the little poppets pictured?
If so we would love to hear from you. Contact Helen Millichamp on 01665 602234 or email firstname.lastname@example.com


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The Dallas Cowboys released all time sack leader DeMarcus Ware due to salary cap issues. It was a sad day in the Big D for all involved with Ware's release. Finding a replacement for DeMarcus is key in the upcoming 2014 NFL draft. Seeing Ware in another jersey, and playing for a different team will be hard for the Cowboys fans to fathom. Here are some of the Twitter reactions from some of Cowboys Nation loyal fans:
Farewell Mr Ware and here's hoping you get placed on a contender. You deserve the best for the passion and love you brought to the sport and the Dallas Cowboys organization. When it's time for retirement, Jerry needs to bring you back for a 1 day contract to retire you with dem boys. The Dallas Cowboys will miss #94.
Check some of his highlights below


text: "Outsource My IT is absolutely the best thing that has happened to me and my computers. In all my years in business I have never found an IT operation that is more responsive, knowledgeable, and service oriented. I have every confidence that Outsource My IT can handle any tech problems that arise and I'm just lucky that I have the Outsource My IT team looking after my tech needs."— Lorri M. Greif, CFRE President, Breakthrough Philanthropy, Inc


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1745 N Main Street
Request a Showing Now.Request Information
Call 864.757.4000
Welcome to 1745 N Main Street.2600-2799 SqFt. In Ground Sprinkler // October 04, 2017
This home is a Three bedroom, Two bath Ranch,Traditional style. The home sits on a 0.45 acre lot.
Traditional elegance in the heart of North Main. Designed by renowned Greenville architect, Willie Ward, this well-maintained 3 bedroom, 2 bathroom home ... Traditional elegance in the heart of North Main! Designed by renowned Greenville architect, Willie Ward, this well-maintained 3 bedroom, 2 bathroom home offers the convenience of one-level living with an open floor plan and an abundance of natural light. Features like 9 ft ceilings, gleaming hardwood floors, detailed moldings and built-in shelving add to the charm. There are two fireplaces, one in the living room and one in the cozy heart-of-pine den. The sunroom, just off the Master bedroom, can be used as a study or a relaxing space to enjoy your morning coffee. A spacious, walk-up attic offers ample storage, but also the potential for expansion. Beautiful outdoor living spaces, like the covered brick terrace and screened porch, are enjoyed virtually year-round. There is also a fenced, landscaped backyard, a full irrigation system and potting shed for the avid gardener. This is a wonderful opportunity to own this fantastic home in the middle of all that downtown Greenville has to offer. Schedule your showing today! High ceilings over nine feet give the rooms an grand impression. Enjoy green grass year-round with the built in sprinkler system that provides full-yard coverage.
Sold for $568,000 on January 5th of 2018.
Priced at $599,900
Greenville IQ™ found 6 other homes you may like...
Map of GreenvilleClick and drag the orange icon
on the left side of the map for Street View.
" data-pancontrol="true" data-zoomcontrol="true" data-maptypecontrol="true" data-scalecontrol="true" data-streetviewcontrol="true" data-overviewmapcontrol="true" data-scrollwheel="true" data-maptype="ROADMAP" data-marker="true" data-style="" data-full="false" >
In-depth Details
Inside the Home
- Attic Stairs Permanent
- Bookcase
- Cable Available
- Ceiling 9ft+
- Ceiling Smooth
- Security System Owned
- Smoke Detector
- Some Window Trtmnts
- Walk In Closet
- Countertops-Other
Outside the Home
- Patio
- Porch-Screened
- Some Storm Windows
- Sprklr In Grnd-Full Yard
- Brick Veneer-Full
Request a showing now.Call 864.757.4000 You may also call our office at 864-757-4000 to arrange a viewing of 1745 N Main Street.
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While the end of the year tends to be slow, Vivisimo announced on December 16th that they have joined the EMC Documentum Application Developer Program.
According to the press release, this new alliance will allow Vivisimo "to ensure that the integration to Vivísimo's Velocity 5 enterprise search platform meets the highest quality standards."
In a recent InfoWorld test center article, Velocity 5 received a score of 8.9 out of 10, placing it at the top of the list of enterprise search software in that publications opinion. The six evaluation criteria were ease of use, integration, management, performance, scalability and value.
Continue reading "Enterprise Search - Vivisimo and EMC Documentum" »


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"My, what nice veins you have!" If you've ever had blood drawn, you know what comes next: tap, tap, tap—Ouch! I'm not especially squeamish, but I do look away to avoid seeing that giant needle and all those vials of blood.
If you find this ritual of our medical system uncomfortable—maybe even ranking up there with leeches and garlic wraps—I have good news for you! Thanks to the dedication and inspiration of a young Stanford dropout turned entrepreneur, you and your veins are about to be saved.
Elizabeth Holmes just turned 30 years old, but is already one of the richest women in the world. Tall and well-spoken, at first glance she doesn't appear to be an obsessively driven entrepreneur and medical revolutionary. However, as she begins to describe her business odyssey—starting a side project as a Stanford sophomore, and eventually dropping out because she was spending so much time speaking with venture capitalists—you quickly appreciate both her talent and determination.
After operating in stealth mode for the past ten years, the company Holmes built—called Theranos—is now fully launched and making waves in the diagnostic testing world. Like other successful game-changing companies in Silicon Valley, she and her team have completely reimagined the entire end-to-end user experience—in this case the experience of having your blood drawn and getting back the lab results.
The Theranos experience begins with a disarmingly simple way to draw blood. A small heat pad is applied to your finger, and then a barely noticeable pin prick draws just one tiny drop of blood. Theranos then places your micro-sample in a specially designed "nanotainer."
From there, your tiny sample is sent to their state-of the-art labs where they have re-engineered dozens of tests to run on small micro-samples of blood. Not only that, but test results are ready in a fraction of the time when compared to traditional labs. The Theranos site describes their process as "Answers at the speed of digital. Waiting for results is often the worst part of getting a test. With Theranos, you and your doctor can get answers back fast. Less waiting in fear. We've designed our systems to help you and your doctor view your results anywhere, anytime, to help you get the care you need when you need it."
Not only are test results completed quickly, but they are delivered and displayed in a modern, remarkably easy to understand online dashboard that is accessible to both patients and healthcare providers.
Theranos is currently in the process of rolling out their testing centers through a major partnership with Walgreens, with initial centers already up and running Phoenix, AZ. Of course, all this technology comes with a price, and the Theranos prices are listed in full transparency on their website.
However, don't expect to be paying more for this advanced testing! That's because Theranos' pricing is in many cases 50% – 90% less than the typical Medicare reimbursement rate. In fact, Holmes estimates that over the next 10 years, Theranos is poised to save government insurance programs in the US hundreds of billions of dollars in testing costs. They are also working with the US military to provide real-time diagnostics in trauma situations.
Theranos has global ambitions as well, and Holmes envisions a future in which accurate and inexpensive access to personal lab results becomes a fundamental human right. A decentralized testing infrastructure, supported by smartphones and Web access, will allow for anywhere lab testing to become a reality, even in the remotest parts of the world. Holmes points out that access to comprehensive testing in remote locations is a critical first step in providing appropriate healthcare delivery.
Looking ahead, Holmes describes that the ultimate mission of Theranos is to deliver universally affordable access to health data, while providing a great user experience, one person at a time. Although it's too early to say whether Holmes and her team at Theranos can succeed in meeting these ambitious goals, the initial results look very healthy indeed…and at least one writer is actually looking forward to having his blood drawn!
Jim Walker
Latest posts by Jim Walker (see all)
- 6 Bio Hacks for Marketing Hyper-Growth - March 21, 2017
- Blockchain and Patient Privacy - March 8, 2017
- Dr. Alexa is Revolutionizing Home Healthcare - February 28, 2017


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A few months ago I read a very nice blog post from our friends at TriLink Biotechnologies giving the chemist's perspective on the excitement surrounding "Click Chemistry" and how it can be used to make non-natural, yet functional DNA and RNA. Some of the terms in that post such as 1,3-dipolar cycloaddition are oriented more towards chemists. Here's a more biologist-friendly explanation of Click Chemistry: [Read more…]
Click Chemistry: What it means for biologists
PEI transfection patents to expire
July 7, 2015 marked the 20-year anniversary of the filing dates of both the U.S. patent and European patent limiting the use of PolyEthylenImine (PEI) as a transfection reagent. Coincidentally, both U.S. and European patents generally have a term of 20 years from the filing date.
Many academic researchers have been ignoring these patents and/or have been sharing protocols online and publishing articles explaining how cost-effective and simple PEI-mediated transfection can be. For example, the most commonly used PEI, catalog nr. 07923966-2 (Polysciences), is a linear form with molecular weight of 25,000 Da. The 2 gram bottle of PEI powder can be used to make a few liters of transfection reagent, so depending on which protocol is used the cost can be about 0.01% that of commercially-available transfection reagents.
PEI is a simple polymer with an amine group and two carbon spacer that is thought to bind to DNA to produce positively charged particles that can enter the cell.
Some of the negative aspects of PEI compared to more sophisticated transfection reagents are:
- PEI tends to be rather cytotoxic. While PolyJet™ DNA In Vitro Transfection Reagent is composed of proprietary bio-degradable polymers designed to greatly reduce cytotoxicity, standard PEI has been shown to induce apoptosis in a variety of human cells.
- PEI is difficult to dissolve. Linear PEI is solid at room temperature and somewhat soluble in hot water and low pH. A typical protocol might involve dissolving 8mg of PEI in 25mL of water, a process that may take many hours or days and/or result in a non-uniform solution that cannot be sterile filtered.
To solve this problem, Polysciences has released the a much more soluble hydrochloride salt form (catalog nr 07924765-2), called Polyethylenimine "Max", (Mw 40,000) – High Potency Linear PEI. This MW 40,000 form corresponds to the MW 25,000 polymer length in free base form, with the salt accounting for the molecular weight difference.
Looking through the literature one might reach the conclusion that PEI is a universal transfection reagent suitable for transfecting cultured cells or use in vivo.
Here's a summary of some of the earlier scientific articles using PEI for various transfection applications:
|Nucleic Acid
|Cell Type
|antisense oligos
|mouse (in vivo)
|Adult neural stem cells
|Brain derived cells
|chicken (in vivo)
|CHO cells
|Cos-7 (nuclei)
|Embryonic neurons (in vivo)
|Fetal mouse liver
|HeLa cells
|Human monocytes
|L929 cells
|LNCaP cells
|Mouse brain
|Mouse lung
|Mouse lung
|Murine adult neural stem cells
|Ovarian carcinoma cells
|Postmitotic neurons
|Pseudocystic tumor cells
|Rat (in vivo)
|Rat brain
|Rat fetal hypothalamic cells
|Rat kidney
|Rat kidney
|WERI-Rb1 retinoblastoma
|Xenopus tadpole brain
|dsRNA and siRNA
|Snail, Biomphalaria glabrata
|modified siRNA
|Murine melanoma cells
|in vivo
|Pancreatic cancer cells
|HT1080 cells
In addition to use as a direct transfection reagent, PEI is used for a variety of "combination" gene delivery methods. Adenofection for example makes use of PEI to permit delivery of large plasmids/BAC/YAC into cells by non-covalently coupling of the DNA to an adenovirus. A variety of other approaches make use of modified (e.g. PEGylated) PEI for gene delivery or have targeted PEI to specific tissues with antibodies or proteins.
In the list of applications, mRNA delivery into cells seems conspicuously absent. Indeed at least one paper indicates that PEI-mediated transfection of mRNA is very inefficient. For mRNA transfection, researchers have better success with the Stemfect™ RNA Transfection Kit or the mRNA-In Transfection Reagent.
Leave your comment below to share your experience with PEI!
CRISPR genome editing: which cell line to choose?
Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.
This technology produces first a targeted break in genomic DNA, which can then be exploited to produce cell lines with genes knocked out or where a donor vector has been used to introduce new genetic elements (point mutants, fluorescent tags, antibiotic resistance cassettes, etc.). Essentially any desired modification to the cells genome can be made. In setting up these genome editing projects there are many choices to be made including vector for the Cas9 protein and for the sgRNAs. Perhaps the most difficult choice, however, can be which cell line to use. Even the most affordable stable genome editing cell line development services can come with a significant cost, so choosing the right cell line at the beginning is crucial. Here we explain some of the choices researchers have in setting up their CRISPR genome editing projects and give our advice for cell line selection.
Kit-Free Site Directed Mutagenesis Protocol
Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol first involves a thermocycling/PCR step with mutagenic primers, followed by a DpnI digestion step to digest the methylated parental/wild-type plasmid, and finally transformation into competent cells for nick repair.
Most experts we've talked to still use this technique, but don't see the point of an expensive kit. Instead they use their own protocols with inexpensive enzymes and reagents bought separately. One such protocol involves the following:
"QuickChange" Protocol for Site-Directed Mutagenesis
Step 1: Design mutagenesis oligos (Primer 1 and Primer 2) using the website PrimerGenesis
Step 2: Order high quality custom oligos from tebu-bio
Step 3: Set up this reaction:
|Plasmid DNA (50ng/ul)
|1 ul
|10x Reaction Buffer (comes with polymerase)
|5 ul
|50 mM MgCl2 (comes with polymerase)
|1 ul
|dNTPs (10mM)
|1 ul
|2.5 ul
|Primer 1 (10uM)
|1 ul
|Primer 2 (10uM)
|1 ul
|AccuStar™ DNA polymerase*
|1 ul
|36.5 ul
|50 ul
* Due to inherent 3'-to-5' exonuclease activity of this very high fidelity enzyme, the polymerase must be added last to the reaction in order to prevent primer damage.
Step 4: Run the following PCR Cycle in a thermocycler
95°C 5 min; 15 cycles of (95°C 30sec, 60°C 1 min, 68°C 15 min*), 68°C 10 min, 10°C forever.
*for large (>9kb) plasmids, increase the extension time to 20min.
Note: run a negative control reaction without polymerase enzyme.
Step 5: Add 1 ul of Dpn1 enzyme to PCR tubes and leave at 37C for 2 hours
Step 6: Prepare a 1% agarose gel with GreenView™ Plus DNA Gel Stain
Step 7: Load 10ul of completed reaction mixed with SafeGreen™ Loading Dye next to 1kb DNA ladder and run the gel at 150V for 10 min to determine if PCR was successful. You should not see a product for the 'negative control' tube that had no polymerase. Troublshooting: If PCR reaction yields no product, then do gradient for the annealing temperature. The DMSO is extremely important, so keep this in the reactions.
Step 8: Mix PCR reaction with 500ul of PB buffer and purify on column. Wash with PE buffer and elute with 50 ul of water. Use 10ul of for transformation of TransforMax™ EC100™ competent cells.
Interested in using this protocol to your lab? Here is a list of products you will need:
|Item Name
|Catalog Number
|2′-dNTP Set
|Accustar DNA Polymerase
|Molecular Biology Grade Agarose
|GreenView Plus DNA Gel Stain
|SafeGreen Loading Dye
|TransforMax EC100 Chemically Competent E. coli
|1kb DNA Ladder
Legal Notes: QuikChange® is a registered trademark of Stratagene.
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such as GeneCopoeia even propose complete cell line generation services. Projects can involve transfection of mammalian expression constructs, TALEN pairs, or direct transfection of RNA.
When scientists want to make a stable knockout cell line, one of the first questions they should ask is whether or not the resulting cell line will be viable. Often a murine knockout mouse has been made and/or siRNA knockdown experiments have been performed in human cells, so experienced users have a good idea if a human knockout cell line will be viable. There are certainly some cases, however, where either the researcher knows or expects that a complete knockout will not be viable but wishes to make the knockout nonetheless. What is the best way to deal with all of the risk involved with starting an relatively expensive and time-consuming project like this that could end in failure?
To knockout an essential (or potentially-essential) gene we recommend the following:
1) Plan the entire project in collaboration with a professional company offering stable cell line development services.
2) Develop your knockout strategy using TALEN or CRISPR.
3) Introduce a functional copy of your gene under the control of doxycycline into a safe harbor knock-in site. This copy of the gene should be designed to be resistant to the TALEN/CRISPR knockout strategy designed in #2.
4) Generate the parental doxycycline-inducible/repressible cell line and isolate a stable clone.
5) Knockout the functional endogenous alleles of the gene in this stable clone.
You will be then left with a viable cell line that will allow you to test whether or not complete knockout results in viable cells. It may be even possible to include loxP sites in the knocked-in cassette to completely remove the doxycycline controlled wild-type gene.
Any questions? Leave your comments below…
BioID proximity-dependent biotinylation studies
In 2012, Roux et al. published a nice paper, that received no less than four article recommendations from F1000 researchers. The paper described a method for tracking the interaction partners a protein has had within a cell (a history of its interacting partners). The method, called BioID, is based on proximity-dependent biotinylation of proteins by a promiscuous biotin ligase mutant BirA (R118G), which is fused to your protein of interest. After an overnight incubation with biotin, cells can be subjected to harsh lysis and biotinylated proteins can be isolated and identified by mass spectroscopy to determine the proteins that had come into contact with the chimeric BirA (R118G) protein. This method is a bit different from standard co-IP or pull-down experiments, because it allows one to identify proteins who interact transiently or weakly with the protein of interest. Also, due to the strong biotin-avidin binding affinity, harsh washes can greatly reduce background protein binding.
We've consulted some experts using this protocol in their labs, and here are the steps required to perform BioID:
1) Obtain pcDNA3.1-myc-BirA(R118G) from an academic collaborator or Addgene
2) Perform or request a custom cloning of your gene of interest (GOI) into the vector EcoRI and HindIII sites to myc-BirA-GOI vector
3) Transfect cells with BirA(R118G) chimeric protein mammalian expression vector using an appropriate transfection reagent
4) Incubate cells overnight in medium with 50 mM biotin
5) Lyse cells directly in 1X Laemmli buffer and sonicate.
6) Incubate lysates with streptavidin-coupled beads for 3 hours at 4°C.
7) Pellet beads and wash 5 times with 1mL RIPA buffer.
8) Elute bound proteins by boiling at 100°C for 8 minutes in 2X Laemmli buffer.
9) Analyze eluted proteins by western blot of mass spec.
Now a number of research articles have been published using the BioID method and have identified novel protein-protein interactions. Some researchers have noted differing results using N- or C-terminal BirA (R118G) tagging. At least part of this might come from the chimeric protein's intracellular localization. Indeed an important step is to confirm that the BirA(R118G) fusion has the same intracellular localization as the protein of interest.
New Fluorescent Labeling and Detection Products
For decades researchers have been using the famous Alexa Fluor® dyes developed by Molecular Probes for everything from flow cytometry to oligonucleotide labeling. These sulfonated forms of common fluorophores (coumarin, rhodamine, fluorescein, and cyanine) are thought to be more stable, brighter, and less pH-sensitive than the native molecules (Ref: Panchuk-Voloshina et al. 1999). Antibody users are likely most familiar with Alexa Fluor® 488 and Alexa Fluor® 594 which when used together allow for the simultaneous staining of one protein in green and another in red and can be used in combination with the blue nuclear dye DAPI.
The original Molecular Probes patents have likely limited the number of companies offering less expensive alternatives to the Alexa Fluor® dyes. Innovative dyes as DyLight® and IRDyes® have been developed and are used for specific applications, but direct alternatives for the standard Alexa Fluor® exitation and emission spectra dyes have been difficult/impossible to find…..until now.
Andy Fluor™ dyes are the next generation fluorescent dyes spanning the visible and near-infrared (IR) spectrum for labeling proteins and nucleic acids:
- Andy Fluor™ 350
- Andy Fluor™ 405
- Andy Fluor™ 430
- Andy Fluor™ 488
- Andy Fluor™ 555
- Andy Fluor™ 568
- Andy Fluor™ 594
- Andy Fluor™ 647
- Andy Fluor™ 680
- Andy Fluor™ 750
For oligonucleotide labeling by Nick translation, Random primer labeling, End-labeling with terminal deoxynucleotidyl transferase, Reverse transcription or PCR amplification Andy Fluor™ dNTPs are a nice low cost alternative to products such as ChromaTide® Alexa Fluor® 488-5-dUTP.
|Amine modification
|Andy Fluor 488
|Andy Fluor™ 488-X-dUTP
|Andy Fluor™ 488-X-UTP
|Andy Fluor 555
|Andy Fluor™ 555-X-dUTP
|Andy Fluor™ 555-X-UTP
|Andy Fluor 568
|Andy Fluor™ 568-X-dUTP
|Andy Fluor™ 568-X-UTP
|Andy Fluor 594
|Andy Fluor™ 594-X-dUTP
|Andy Fluor™ 594-X-UTP
|Andy Fluor 647
|Andy Fluor™ 647-X-dUTP
|Andy Fluor™ 647-X-UTP
Originally developed by Applied BioProbes, the Andy Fluor™ dye labeled nucleotides are a small part of the rapidly growing fluorescence-based molecular biology offer from GeneCopoeia, Inc. Just as Alexa Fluor® has been the market leader for covalent fluorescent labeling of antibodies and oligonucleotides, SYBR® Green I, also produced by Molecular Probes, has historically been the leading dye for qPCR and "safe" agarose gel staining. GeneCopoeia has recently released their own: GreenView, GreenView Plus, and RedView agarose gel dyes.
GreenView and RedView DNA Gel Stains very popular alternatives to Ethidium Bromide, as SYBR® Green-based Gel Stains have been demonstrated to offer a better safety profile by Ames test due to reduced DNA intercalation (Ref. Singer et al. 1998). Further optimization has resulted in dyes that also fail to cross the cell membrane of living cells resulting in an improved safety profile:
Additional innovations from GeneCopoeia include the iQuant™ DNA Quantitation Kits to allow precise quantitation of DNA samples across a wide range of concentrations. Fluorescence-based DNA quantitation is highly sensitive and selective for DNA, relative to NanoDrop®-based absorbance readings (e.g. 260/280 ratios). Kits exist for double-stranded DNA or single-stranded DNA and can be adapted to microplate format using a fluorescence plate reader or tube format using fluorometers such as Qubit® or Quantus™:
Legal notes: Alexa Fluor®, SYBR®, Qubit® and ChromaTide® are registered trademarks of Life Technologies Corporation. DyLight® and NanoDrop® are registered trademarks of Thermo Fisher Scientific. IRDyes® is a registered trademark of Li-COR. Andy Fluor™ is a trademark of Applied Bioprobes. Quantus™ is a trademark of Promega.
Column-free, Caustic Solvent-free DNA or RNA Purification
Epicentre® (an Illumina Company) has developed a series of MasterPure™ DNA and RNA purification kits for nearly any sample type. While Epicentre's QuickExtract™ DNA Extraction Solution is designed for the fastest extraction of low abundance DNA for PCR, the MasterPure™ kits yield the highest quality DNA and RNA for applications such as:
- NGS genomic DNA and RNA-Seq library preps
- DNA methylation/epigenomics studies
- Genomic DNA and cDNA cloning
- qPCR and qRT-PCR
These kits offer a simple protocol for the purification of Genomic DNA, cellular RNA, or total nucleic acid (TNA):
PseudoU-RNA Molecular Weight Markers
Standard RNA ladders such as the DynaMarker® for large or small RNA or double-stranded size determination are routinely used by researchers wishing to confirm the molecular weight of an in vitro transcribed or chemically-synthesized RNA. Due to the altered gel mobility of pseudouridine-containing RNAs, a PseudoU-RNA Molecular Weight Marker can be used to more accurately assess the molecular weight of these modified RNAs.


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In one of several small studies reported at Digestive Disease Week here, an open-label pilot trial of high-dose (800-1,200 mg daily) of Xifaxan found that six of seven patients randomized to 400 mg bid reported improvement in symptoms. So did three of seven patients who received the higher doses, said James George, M.D., of Mount Sinai Medical Center in New York, and associates.
Dr. George said he and colleagues investigated Xifaxan following the report that a 10-day course of Xifaxan at 200 mg TID demonstrated some activity for patients with small intestinal bacterial overgrowth. There was, however, no statistically significant improvement among small intestinal bacterial overgrowth patients treated with Xifaxan.
To be eligible for Dr. George's study patients could not have any other co-existing gastrointestinal disease, recent antibiotic use, or be taking Zelnorm (tegaserod). Dr. George's team enrolled 14 patients with constipation-predominant IBS (IBS-C) who tested positive for bacterial overgrowth. Seven were given Xifaxan at 400 mg BID and seven were given 400 mg TID for 10 days.
Six of the seven patients on the lower dose and three of the seven on the higher dose reported improvement in bloating and constipation. Symptoms improved within a week of starting treatment, but six patients had symptom recurrence within two weeks of completion of the 10-day course. All six responded to a second course of Xifaxan. The remaining eight patients had continued relief of symptoms for the next four weeks after the completion of the treatment course.
Xifaxan, which is FDA approved for treatment of noninvasive Escherichia coli, was well-tolerated by all patients in the study.
Led by Ernesto Lauritano, M.D., investigators from Catholic University of Rome used a glucose breath test to assess results of a dose-finding study of Xifaxan for treatment of c-IBS with small intestinal bacterial overgrowth. They defined as peak H2 levels 10 ppm above the basal value after ingestion of 50 g of glucose.
Dr. Lauritano and colleagues randomized 60 patients into two treatment groups. One group received Xifaxan at 1,200 mg daily and the other at 1,600 mg daily for one week. The glucose breath test was repeated one month after the end of therapy.
The glucose normalization rate was 60% using an intent-to-treat analysis in the low-dose group and it was 63% in the high-dose group.
But the researchers pointed out that higher-dose Xifaxan was not associated with higher efficacy and as the dose increased, so did toxicity.
A similar study was conducted by Mark Pimentel, M.D., of Cedars-Sinai Medical Center in Los Angeles, and colleagues.
The investigators enrolled 87 patients with IBS-C. At baseline and after treatment patients were assessed with a structured questionnaire rating severity of abdominal pain, bloating and constipation. The patients were also assessed using a lactulose breath test, similar to that used by the Italian team.
The patients were randomized to Xifaxan 400 mg or placebo TID for 10 days.
One week after the antibiotic course, 48.6% of patients on active treatment had a significant response in symptoms of diarrhea compared with 23.5% with placebo (P<0.05). Moreover, 34.9% of Xifaxan patients had improvement in symptoms of bloating compared with 18.6% of placebo patients but this was not statistically significant (P=0.07).
Constipation and abdominal pain were unchanged at this assessment point.
At the end of the 10-week study, all four symptom categories showed improvement, including 35.1% of patients on active treatment reporting improvement in constipation compared with 28.1% on placebo (P<0.05) and 39.7% of patients on Xifaxan reporting improvement in abdominal pain compared with 28.7% on placebo (P<0.01). Improvements in bloating (49.2±6.8 vs. 22.6±3.5%, P<0.0000001) and diarrhea (50.6±5.7 vs 35.3±6.3%, P<0.00001) remained highly significant.
- Reviewed by Zalman S. Agus, MD Emeritus Professor at the University of Pennsylvania School of Medicine
Primary Source
Digestive Disease Week 2006Source Reference: George J et al "An Open-Label Pilot Trial of High Dose Rifaximin in the Treatment of Patients with Constipation Predominant Irritabe Bowel Syndrome" Abstract T1287
Lauritano E et al "A Dose-Finding Study of Rifaxamin in the Treatment of Smal Bowel Bacterial Overgrowth in Patients with Irritable Bowel Syndrome" Abstract M1174
Pimentel M et al "A 10-Day Course of Rifaximin, a Non-Absorbable Antibiotic, Produces a Durable Improvement in Symptoms of Irritable Bowel Syndrome: A Double-Blin Randomized Controlled Study" Abstract 134


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- JotForm SupportWelvinAnswered on September 17, 2015 at 05:56 PM
Hi Tim,
No, you don't need to re-embed the form if you are using our default embed method. The changes will automatically appear in the embedded form as soon as the form is saved.
- anubisairdogsAnswered on September 17, 2015 at 07:47 PMThanks
- JotForm Supportashwin_dAnswered on September 18, 2015 at 06:11 AM
Hello anubisairdogs,
On behalf of my colleague, you are welcome.
Do get back to us if you have any questions.
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RAIN-WANA is open to participation by all interested agricultural research centers, projects, and national libraries in the WANA region. As a member of the platform, you can store and share all your information publicly and privately – including publications, technical reports and information, photos, brochures, case studies – and share it with the world.
For more information or to participate contact:
Michael Devlin: m.devlin [at] cgiar.org
Nihad Maliha: n.maliha [at] cgiar.org


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Oil was up about 6 percent on Thursday as rising violence in producing country Libya and an expected boost in oil demand from a central bank easing in China helped crude rebound from one of its sharpest daily routs ever in the previous session.
Continue Reading Below
Traders and analysts said they expect higher-than-usual volatility in coming days as the market tries to find a bottom to a seven-month selloff that took prices to near six-year lows.
But many were pessimistic about the market making a sustained rally, with record-high U.S. crude inventories rekindling renewed worries about a supply glut.
"It is just a changing market sentiment as more and more players are starting to believe production cuts are coming in the U.S. and that will be enough to erase the surplus," Dominick Chirichella, senior partner at the Energy Management Institute, New York, said on the Reuters Global Oil Forum.
"I think there will be a lot of disappointment going forward for that view."
Benchmark Brent crude futures were up $3, or 5.5 percent, at $57.16 a barrel by 1:02 p.m. EST (1802 GMT).
U.S. crude futures, also known as West Texas Intermediate (WTI), were up $3.16, or 6.5 percent, at $51.61.
Brent fell nearly 7 percent and WTI 9 percent on Wednesday after the U.S. government reported a 6.3 million-barrel build in U.S. crude stocks last week to above 413 million, their highest since 1982. The report cut short a four-day rally had sent crude prices nearly 20 percent higher.
Prices were up from early on Thursday, reacting to a raid on an oilfield in Libya by gunmen even though production was not affected.
An attack on a tanker off Nigeria and monetary easing by China that could boost on oil demand also provided support to crude.
The rally accelerated towards noon, after oil services firm Genscape reported a smaller-than-expected build in crude stockpiles at the Cushing, Oklahoma, delivery point for WTI in the four days since Jan. 30.
Market sources who saw the Genscape data said it estimated a build of about 550,000 barrels at Cushing. Traders had expected a spike of more than 1 million barrels in that period.
The smaller-than-expected Cushing build sharply narrowed the discount in WTI's front month to the second month <CLc1-CLc2>. Brent premium's to WTI <CL-LCO1=R> meanwhile, rose to over $6 a barrel, its widest since early November.
(By Barani Krishnan; Additional reporting by Alex Lawler in London and Jacob Gronholt-Pedersen in Singapore; Editing by William Hardy, Jason Neely, Meredith Mazzilli and Marguerita Choy)


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John K Buor
Ghana Institute of Management and Public Administration, Ghana
Title: Effect of frequent policy change on disaster preparedness and systemic resilience
Statement of the Problem: Reducing exposure to hazards, lessening vulnerability of people and property, wise management of land and the environment, early warning, and improving preparedness for adverse events are all pre-requisites leading to disaster risk reduction. However, it appears that key industry players and stakeholders are not able to anticipate the effects of strategic risk management decisions. Blindfolded by cost management tendencies, supplies of resources leading to disaster risk reductions have kept dwindling over the years.
Methodology & Theoretical Orientation: We employ system dynamics (SD) modelling, with data sourced from elitist interviews, to analyse the structural behaviour of the interactions between Disaster Preparedness, Systemic Physics (or the physical environment), and Systemic Resilience in response to policy change in maritime logistics setting.
Findings: Results from our research suggests that frequent policy interventions geared towards disaster reduction has the potential to produce unintended consequences as levels of increased unacknowledged conditions.
Conclusion & Significance: Our research models therefore, could provide strategic policy makers with real-time decision evaluation tool to justify the choice of a set of alternative risk reduction interventions prior to decision implementation.
John K Buor is developing expertise in his research into effects of policy change on logistics/supply chain risk, security, emergency, and disaster/disruption management leading to uninterrupted flows in global logistics (i.e. hazard management and disaster preparedness). His research philosophy is founded on Giddens' (1979) theory of structuration. He employs hybrid methodological approach (system dynamics simulation modelling techniques) to model the potential consequences of policy interventions on the structural behaviour of the interdependencies between/among factors that can influence global logistics/supply chain resilience and sustainability with a keen interest in promoting green logistics/supply chain and environmental safety


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The purchaser, Mik Jac Properties (Pty) Ltd (seller: The Antonio Da Costa (Sr) Trust), plans to demolish the existing structures at 156 Leeuwpoort Street (Erf 1774), Boksburg, Ekurhuleni Metropolitan Municipality. In terms of Section 34 of the National Heritage Resources Act, Act 25 of 1999, an application for the above must be submitted to the Provincial Heritage Resources Authority of Gauteng (PHRA-G).
Any Interested and Affected party (I & AP's) who wishes to comment on this is invited to do so in writing to the Provincial Heritage Resources Authority of Gauteng (PHRA-G) at Private Bag X 33, Johannesburg, 2000/ e-mail: firstname.lastname@example.com (please cc email@example.com)
Closing date for comments: 8 February 2018
Disclaimer: Any views expressed by individuals and organisations are their own and do not in any way represent the views of The Heritage Portal.


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To be fair, I have really always been digital. I started recording by plugging my bass into a Boss distortion pedal (fondly named 'Captain Crunch') and that into the line-in on the back of an old Mac. It sounded terrible, but that's where I got my start. Now I have professional equipment, both digital and analog. There are pluses and minuses to each, and quality good sounding gear is available in abundance for both types. However, there is something a bit more fun to the analog world.
Generally, I've strived for a 'hybrid' approach. I have a Soundtracs Topaz mixing board that I use for mic preamps and EQ before converting to digital audio and multitrack recording on a computer. It's basically the old analog workflow except I replaced a tape machine with an analog to digital audio interface and a computer. The idea is to then do any editing on the computer and add any effects, then run everything back out to the analog mixer to build a final stereo mix. That last step has never really happened. I don't have enough analog effects to really do much other than basic EQ and setting volume. Even though I have been mixing 'in-the-box', I've still always imagined and planned my studio and gear with a hybrid approach.
The majority of my paying gigs have always been live recording. For that the smaller, lighter, and less gear I have to take with me, the better. An analog compressor will take up likely a whole space in my rack and weigh a number of pounds. A digital plugin compressor, only takes up an unnoticeable amount of hard drive space once, but can be used an unlimited amount of times simultaneously. Generally, it's also cheaper to buy a good sounding quality plug-in than it is to buy it's analog counter part.
Now, I still WANT the analog gear. It looks cool to have racked up. I like plugging things together and adjusting knobs and seeing the lights blink in rhythm with the music. It's just not the same to see a graphic representation on a computer screen. For my true needs, though, software is much more efficient and effective.
So I am finally fully embracing the convenience and cost effectiveness of digital. It's more of a mindset, really. I've pretty much been fully digital, anyways. I'm actually a little relieved to not be striving for things that just aren't currently the right fit for me. I would still love to someday get nice hardware compressors and EQs and mix on my board. For now, I'm going to just go with what makes sense.


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Oswestry's Hope House is looking for energetic people to sign up to their sporting fundraising events for the coming year.
The hospice is looking for people to form part of Team Hope House and Ty Gobaith in 2018 as the children's charity back a range of events from half marathons, aquathons and triathlons as well as organising their popular annual Cycle Challenge and their brand new Snowdon Trek.
Both the Chester Half Marathon and Shrewsbury Aquathon on April 29 are in Hope House's sights and if anyone has already registered for these events or wish to register for an event not listed, they can still become part of Team Hope House and Ty Gobaith by committing to raise minimum sponsorship.
The first ever Hope House and Ty Gobaith one day Snowdon Trek will take you to the summit of the highest mountain in England and Wales, Mount Snowdon, on Sunday July 1.
The trek will follow the most popular route to the summit, the Llanberis Trail, with a team of experienced mountain guides, guiding you along the route at check in points. Registration is £20 plus £100 sponsorship per person (to include free t-shirt).
The annual Hope House and Ty Gobaith Cycle Challenge reaches its 10th anniversary in 2018 and will take place on Sunday August 5.
The popular ride will be going back to its original two scenic routes – 56 miles to Lake Vyrnwy and 72 miles to Bala Lake. Cyclists will be treated to a snack stop at a Half Way Feed Station and a delicious home-made feast of cakes and savouries at the end.
Cyclists can register for the early bird price of £25, on the day entry will be available at £35.
If you would like to sign up for any of the events visit www.hopehouse.org.uk/whatson or call the fundraising office on 01691 671671 / 01492 596 581


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Tuesday, April 10, 2012
Capitalism: Dukkha par excellence?
1 comment:
all good thoughts, but i would just point out that neither buddhism nor capitalism is a single entity. both can be and are imbricated systems, and buddhism has very frequently done very well in market economies -- witness how it spread across asia via the silk road and sea trading routes. of course, buddhism is changed as it comes into contact with new cultural forms and modern capitalism is no exception. but the important question you raise, i think, is whether we as modern persons within the systems of modern capitalism and modern buddhism can learn to recognize the ways in which our approach to meditation or other forms of buddhist practice is perhaps less revolutionary than we like to imagine and more caught up in the cultural forms that do keep us docile, with our craving present but controlled in part because we have not sufficiently cultivated discontent with the entire craving-consumption-craving complex.
- April 12, 2012 at 7:38 AM


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This forum is for you to use as you wish for asking questions or generating discussion on non-motorized use of trails in Kananaskis Country or of issues relating to trail use. Where a blog has been linked to the forum, please follow the link at the bottom of the blog to the forum to make comments.
4:54 pm
December 21, 2012
I have been working through all of the hikes in the "Where Locals Hike in the Canadian Rockies: Kananaskis" trail guide and I should be done the book by end of summer 2013. The only one left that I'm equally excited and terrified to do is Northover Ridge. Debating the pros and cons of tackling it in one day. Planning to assault it in August of 2013.
I have a neck/shoulder injury that makes backpacking difficult. I can backpack, but even on level ground with only 35-40 lbs on my back, my neck/shoulder are screaming within a few kms. The idea of carrying that weight over a razor-sharp ridge worries me.
I have done long distance hikes, and have little trouble covering 30+ km in one day if I'm carrying minimal gear. I'd like to hear from people who have done this ridge in one day, and what their challenges were, how long it took them, whether they recommend it, etc.
I am a very experienced hiker and cover about 500 km in the four-month hiking season, but the biggest ridges I've done to date are moderate ones like Akamina, Lineham, Pocaterra, and Mist. Any cautions or words of wisdom would be appreciated!
12:52 pm
December 8, 2008
Hi GG, The ridges you list are all very easy. Northover is much more serious. I would suggest that before tackling it you get some harder trips under your belt, ridges or easy ascents involving lots of scree and easy scrambling with some exposure.
My own view is that it seems a huge pity to condense 2 or 3 enjoyable days in the mountains into 1 day when you are not going to be able to stop and look at anything. What about carrying lightwight bivvy gear?
Anyone else with ideas?
1:25 pm
My first reply disappeared into thin air!
I did this trip many, many years ago when I was much younger and fitter (1 Sep. 1984!).
We started from the Upper Lakes parking area, followed the trail around the south side of Upper K Lake, to Hidden Lake, then up the steep trail to Aster Lake. From there we hiked up through snow to the ridge, which we followed until dropping down to Three Isle Lake. Then it was a long, fast hike all the way back, down the headwall and around the north side of Upper K Lake and finally back to the car. We figured it was about 34 km and just less than 4,000 vertical feet. It took us 12 hours 40 minutes, and we started at 7 am.
You would need a nice sunny and long day to do this trip in one day. There are some tricky sections, such as the route up from Hidden Lake to Aster Lake, and along the ridge itself. We almost wandered out onto the glacier in a strange mist which appeared out of nowhere. We found a lost guy up there with a huge backpack who we added to our party. We had day packs plus some extras. The ridge has a knife edge section which might intimidate some people, especially if windy.
My diary reads: "Over 12 and a half hours of continuous walking, with only a few breaks...quite a marathon day!". I wouldn't go up there with a sore neck - it will get a good workout twisting around to let you admire the awesome scenery up there. Since my experience dates quite far back, there will be plenty of others who will give you better advice than me. But I did make it round the circuit , so it can be done!
Good luck.
10:41 pm
December 8, 2008
6:58 am
8:10 am
I'll add one suggestion by Bob, which he has just again reminded of via email, and that is to consider going in via Aster Lake and continuing out via Three Isle. Two reasons. The first is that the braided streams at Aster Lake fill by the afternoon, thereby slowing you down. The second is that there is no bushwacking once you are at Three Isle, and this may be important if you are late.
The third reason I'll add is that if the water level in Hidden lake is high, you'll only have to do that section once.
11:27 pm
April 15, 2010
I'm a fan of backpacking, and of taking one's time to see an area, but I think it can also be really neat to cover a lot of ground and see a ridiculous amount of scenery in a day trip. I can definitely see the logic given your issue with backpacks.
For this one, you need to be comfortable with both the distance and the terrain.
I'm not sure what else you've done, but easy-to-moderate scrambles including exposure would be a good idea. Lady Macdonald would be a good testpiece for the terrain.
For the distance, do the Iceline first as a testpiece.
Northover Ridge on the WLH book is stated as 33.7km, 1180m gain.
The Iceline Loop (full loop, not dayhike trail) is 30km, 1100m gain.
The Iceline also has good trails the entire distance, compared to the mix on Northover.
If you have any trouble with the Iceline, do a few more trips before Northover. If you can do the Iceline with plenty of time to spare, Northover seems like a comfortable next step, assuming you have experience on comparable terrain (routefinding, exposure).
Watch the conditions too, as you have to balance the early season long light with the late season easier route around Hidden Lake. If you do go, definitely start on the Aster side and get the challenging part over with. On the Three Isle side, once you make it down the headwall, the last 8km are easy, and the last 6km or so are big wide open easy trail. That's nice to have waiting at the end.
Given the need to start early and possibly end late (thus moving around twilight), it would be good to plan around the avoidance of heavy berry season as well.
8:12 pm
Are u sure iceline loop is 30 km? I did a hike/jog on that trail in 5 hours, the full loop....is northover ridge really comparable to the iceline? I really want to do this hike but like most worrie about time. In the pics it almost looks llike a 30 km hike with a constant incline.
Any help on this would be great. I am in good shape and do tend to do some jogging on trails.
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Oh these little ones! Every year, they crack me up. At first we managed to be semi-serious.
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Kym Lomas has taken a well deserved break from 'Coronation Street' to enjoy a girly holiday in Marbella, where she has been pictured showing off her amazing bikini body.
The former Hear'Say singer jetted off with former WAGs Nicola T and Elen Rivas and ex-'Emmerdale' and 'Hollyoaks' star Sheree Murphy.
Kym was seen lazing around the pool with her gal pals in a pink two-piece, which revealed her killer curves.
Meanwhile, friends of the star are said to be worried about her marriage to Jamie Lomas after he joined rival soap 'EastEnders'.
The couple will be forced to spend five days a week apart, as Kym's on-going role as Michelle Connor in 'Coronation Street' means she will continue to be based in Manchester, while Jamie will be filming 'EastEnders' in London.
A source told The Mirror that it could spell trouble for their relationship, which already came under strain when Jamie unsuccessfully tried to crack America.
"It will often mean spending five days a week apart, and friends are saying that it's only a matter of time before the cracks begin to show again," they said.
It was revealed last week that Jamie will be playing recovering alcoholic Jake Stone, who will embark upon an affair with troubled Lauren Branning, who has been facing her own battle with the booze.
"I have admired the show for many years... Jake Stone is a great character to play and I'm really looking forward to getting my teeth stuck into the role," Jamie said of joining the soap.
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