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Michael Clark Proves That (20/10 Vision) Does Exist In The Basketball World
If you don't know Michael Clark then it's about time you get...
MIAMI (BASN) — In my years of following the U.S. National Team I've experienced joy, frustration, disappointment, exhilaration, pride and sometimes anger.
Never have I felt embarrassed.
But that's how I felt after watching the US' performance against Brazil in the Confederations Cup. This is worse than the loss to Iran in the 1998 World Cup. As I write this the US is still technically alive in the Confed Cup and I realize that we haven't even played Mexico in Azteca yet but this feels like rock bottom. As of right now, this is not a Top 15 program.
Team USA simply doesn't get it.
No, not just the players. Fans. Coaches. Pundits, like myself.
U.S. Soccer simply doesn't get it right now.
See, it's easy to lay this on the players. They are the ones who have been going out there day after day and playing substandard football since about this time last year. The 2002 World Cup success seems as distant as my high school days.The 2007 Gold Cup seem like a decade ago.
Since then there hasn't been much to hang our collective hats on and say, "See, this program is going somewhere."
But it goes beyond them. See, I am so over hearing this talk about hard work and determination. About getting "stuck in" and how we don't need more athletic players who can do fancy things with the ball. I'm so tired of hearing about false positive results like squeaking by Cuba and claiming historic points on the road against Guatemala.
Do we get it yet? Do we get that EVERYONE plays hard at the top level of the game? Do we get that EVERYONE is determined to win and to make their mark in the world of international football?
Do we understand that it takes players who have enough skill and confidence on the ball to not give it away any time they get under pressure (or even not under pressure such as on a short corner kick), especially against real teams like England, Spain, Italy, Brazil or away to Costa Rica and Mexico?
Do we understand that even when under pressure defensively that top teams like Brazil clear it to teammates instead of simply hoofing it because they are able to keep their composure?
Do we get it yet that against a sound resolute defense like Italy skill and the ability to unbalance a defender, not for the sake of doing a trick, but to create a defensive breakdown is what wins games at this level?
Skill and creativity (similar to what we saw on Brazil's third goal) are not surplus to requirements but is something rather basic amongst top professionals. We can't seem to break anyone down with ball at our feet and then wonder why we can't make an impression against the top sides.
We bristle when it's suggested that Americans can't play this game yet how often does American player do something with the ball to make you get out your seat and shout "OLE!"? Former Manchester United midfielder Roy Keane gets it. As he said a year or so ago when England failed to qualify for Euro2008, "You have to have the heart and passion but that only gets you so far…"
Do we get it yet that there is no reason for a player with the ball at their feet on a counter attack, no matter how talented, should be able to outrace players from our country that has produced so many world class athletes? Do we see it now?
Do we now understand that players have to be put into a position where they can succeed? Isn't it becoming clear that looping a ball into the air and expecting a 19 year old kid to bring it down and hold it amongst 3 defenders, hoping someone comes to help him is neither a good use of the ball or his skill?
Did anyone else notice that he created a penalty when the ball was played to him ON THE GROUND and then was able to size up his defender? Do we see now that playing players out of position who aren't getting time at their clubs or giving someone their first World Cup Qualifying start in the 2nd toughest stadium in CONCACAF because he plays on turf isn't going putting your team in a position to win?
When possession is necessary or you need a midfield spark is keeping players like Jose Torres and Freddy Adu on the bench really giving your team the spark it needs?
Do the leaders of this team now know they need to inspire their team? Do fringe players realize they have to step up when they're presented opportunities? Do players realize that the US does not get calls?
No, neither of the cards shown to Ricardo Clark and Sacha Kljestan should have been red but that doesn't excuse putting yourself in a position where you can be penalized as such. Have we learned this yet? Have we figured out that we need a purpose on offense, that forcing passes, failing to switch the field to maintain possession simply puts the team on the back foot?Do we in the media now realize that we may have shirked our responsibility in demanding better results? Are we afraid to call out players and coaches as it's done in other sports here and other footballing nations because we fear being shut out of the small soccer media circle?
I'm not suggesting coaching witch hunts but by putting polyurethane on substandard CONCACAF performances or excusing embarrassing displays because they were just friendlies did we fail to put the decision makers on notice that this run of results dating back to last year simply wasn't acceptable?
Do we get it yet?
I want to believe that we do now. I want to believe that we've taken the collective experiences of last year's friendlies, the ho hum results against sorry teams in qualifying, the inconsistent play of the Hex and the harsh reality check dealt to us in this Cup and will collectively raise this program to levels. NOBDOY that we play from now until the end of our run at the World Cup is fearing us right now and that has to change. It's important that we understand that now.
I hope we win the Gold Cup but for this Grown Man, the road to recovery begins in an unlikely place, Estadio Azteca. How the US performs there will give us an idea as to whether we've reached the understanding necessary to make this a team to be reckoned with in South Africa next year or more of what we've been seeing for the past year. We're less than a year away from the Big Show but right now, we lack the collective understanding of what it takes to perform on that stage.
"You get it now, do ya?"-Timothy Delahunt, The Departed


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'Stating the obvious!' Brexit no deal papers blasted for offering 'few solutions'
Economist Julian Jessop fears a Brexit "in name only"
BRITAIN faces a "Brexit in name only" according to a top economist who says the Government's list of no-deal technical notices published last week amounted to a "long list of problems with very few solutions".
Julian Jessop, chief economist and head of the Brexit unit at the Institute of Economic Affairs, said the Government did little more than "state the obvious" when it released 25 guidance documents advising people and businesses in the event of a no deal.
He said: "In some ways it is a good effort – it's about time the Government started talking about these things.
"The thing is you can explain the implications of what a no-deal scenario would be but most people know that already. It's stating the obvious.
"What they want to know is what the Government is going to do.
"It seems to me to be a long list of problems offering very few solutions."
Mr Jessop said part of the problem was the deep divisions which exist within Mrs May's Government between what he termed soft and hard Brexiteers, which are reflected in Government departments.
He said: "Basically the soft Brexiteers want as soft a Brexit as possible, probably remaining in some sort of customs union, and the hard Brexiteers are not going to be diverted in any way."
He suggested Remainer Philip Hammond's decision to warn that a no-deal situation would cost the UK economy £80billion on the day Brexit Secretary Dominic Raab unveiled the technical notices were deliberately timed.
Theresa May is being hampered by divisions within her Government, Mr Jessop said
He said: "Number 10, the Cabinet Office and the Treasury are all keen on a soft Brexit.
"The Department of Trade and the Department for Exiting the European Union are less so.
"There are two sides here which is why we are seeing conflict.
"If you had asked me a year ago I would be pretty relaxed about a no-deal situation.
Mr Jessop believes Chancellor Philip Hammond's timing was deliberate
"But the clock is running down pretty quickly.
"Michel Barnier hinted there might be some of unique deal in the offing but we knew that already.
"I worry that we will end up with a Brexit in name only, were we are close to the rules of the single market, which would likely scupper hopes of a trade deal with the United States for example."
After the latest round of talks between Mr Barnier and Mr Raad, the former said said it was "possible" to get an agreement in time for a summit of all the bloc's leaders in Brussels on Oct. 18-19, though he said delay into November was also possible.
Meanwhile Mr Raab said he was "stubbornly optimistic" and "as confident as before, if not more" that there would be a deal.
The two said they made progress over security cooperation, including on exchanging data.
Speaking at a joint news conference, Mr Raab said: "Europe's security is the United Kingdom's security."
However, Mr Barnier said unresolved issues include geographical indications, the role of the EU's top court in policing the agreement, Euratom cooperation and data protection, among others.

subset: commoncrawl


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This year I have been able to obtain far more divisions/offsets from my mother-plants than in other years, the only problem now is that I have over 4000 auriculas to find space for.
Previously I lived in Sweden for nearly 20 years and my quest there was to hybridize auriculas to produce ever better plants suitable for the harsh Swedish climate. Whilst living there I also created and run Sweden's Auricula Club. I've now returned to England to continue growing auriculas together with my daughter-in-law, Iris. Iris is also an enthusiastic & skilful Iris grower; many of her wonderful Irises can be seen on this blog.


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Photo copyright Chaz Boston Baden unless otherwise specified. Scroll down and send a message via the caption-correction box to request permission to use.
Sherri's t-shirt says "I am not shitting you."
(2005-05-28 01:25:00)
Photos by Chaz Boston Baden.
Caption help for this group of 10 photos received from Rhonda Eudaly.
Back to ConQuesT 36 part 11: Friday Late Night (28-May-2005)


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As I sit here at my desk on my lunch break eating my pasta with seafood and veggies that I made last night, all ingredients bought from the grocery store without even giving it a second thought, I've been thinking. Everyone's been asking me these days…"What? You're not keeping track of the games?" No, I'm not worried about the Flames, not this year or really any year, not even when I've been in box seats during all the play off games…but even then I walk out of that room wondering who won anyways? I'm worried about global food prices. Forget the Flames and their million + they get a year to work a sport that they love…I know they need a job too but what I'm talking about here is something that's deeper than entertainment. It affects all of us on so many different levels. I mean, don't get me wrong, yeehaw for Canada and it's rising grain prices! The Farmers are sure happy and keeping busy and we certainly aren't short of food, just notice the pricier milk and bread nowadays but overall we're doing well. But what about the less fortunate that can't afford it now?
What about the rest of the world? What about Haiti? They're already the poorest country in the western hemisphere, my heart goes out to them because they now rely on dirt as a source of calcium (yes, dirt!) and even the more poor rely on mud as a daily staple in their diet. In Egypt, people are dying in line while they wait for subsidized bread. Dying in line for SUBSIDIZED BREAD! I cried when I read that and I can barely type this note now without tears falling on my keyboard. If mother nature is the cause for all of this then what does that mean for the future? The question isn't what is the world coming to? It's what has this world come to? We're all part of it. Yes, even you and you don't even realize it even though it's not intentional. It's the cycle of life.
The price of food has risen drastically and we don't see it because it's not advertised in our face like gas prices. We don't see the people in other countries suffering because where they live and grow their food they are being taken advantage of and practically raped. Have we fought enough over oil that we can't fight anymore so let's move on to something else? Food? This planet is supposed to hold a common human species that should love each other and work together but we want to kill each other for what we have, how immoral. Obviously the reason why the world is suffering because there's so much primitive activity reverting back to Neanderthal days I think. Sometimes I look into the stars at night and think that maybe I was put on the wrong planet. I have my own problems and times are tough being a single mom and taking care of my mother and just getting by (thanks for reading), but when put into perspective I have my health, I have the best son ever, my mom is alive, I have a great job, my friends are my family, I have a roof over my head, food in the fridge and a not bad shoe collection! So next time when the when you're having a bad day, smile about it because I bet you could make a list like mine of all the wealth you possess. And when the Flames aren't doing so well, just remember that team that got booted out before was even worse.
So if you don't want to be a part of the evil in this world, or better yet, you want to be part of the angel team, then stand up and use your voice!!! Make a difference even if you think it's a small gesture; it all adds up. Next time when you're at your favorite grocery store, notice the prices of food and buy a damn $5 hamper!!! Not only today, but everyday, be grateful for everything that you have even if you think it's less than what someone else has or deserves. Because what someone else has I'm sure they deserved too. One of the hardest things for people to do is be sincerely happy for others. How can you be happy for someone if you can't be happy for yourself first? Do something nice for someone today. Tell someone you love them. Just do it…don't hold back. Why are people so hesitant on telling someone they love them but can instantly tell someone they hate them. I love you and thank you for reading! Sorry so short, lunch is over and I'm going into a meeting. (This was true but as the Virgo I am a had to just read over it before publishing so just getting it out now rushed.)
Love. It's the highest frequency. Tune in sometime.
~Rina xoxo
The world isn't running out of gas or food, it's running out of souls. Be a star and light up the world…every star counts.


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"Jinx Removing" t-shirts. This is an ode to the classic "Spell Breaking" seven day candles, designed with love and good intentions for you and your closet. Bring on the good luck this summer.
We're note superstitious, just a little-stitious.
100% cotton. Hand-printed in Toronto, Canada, in a limited run. Available in black with white print on chest and back.


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|"Wendy Smoak (JIRA)" <j...@codehaus.org>
|[jira] Updated: (CONTINUUM-1689) Bad string replacement of entities in build result
|Thu, 10 Sep 2009 05:16:31 GMT
[ http://jira.codehaus.org/browse/CONTINUUM-1689?page=com.atlassian.jira.plugin.system.issuetabpanels:all-tabpanel ] Wendy Smoak updated CONTINUUM-1689: ----------------------------------- Affects Version/s: 1.3.4 Fix Version/s: 1.4.x Confirmed in 1.3.4 by adding -X to the arguments of a simple project. For example, instead of {noformat} OS name: "mac os x" version: "10.5.8" arch: "x86_64" Family: "mac" {noformat} the output has {noformat} OS name: "mac os x" version: "10.5.8" arch: "x86_64" Family: "mac" {noformat} and Instead of {noformat} [DEBUG] Configuring mojo 'org.apache.maven.plugins:maven-clean-plugin:2.2:clean' --> {noformat} the output has {noformat} [DEBUG] Configuring mojo 'org.apache.maven.plugins:maven-clean-plugin:2.2:clean' --> {noformat} > Bad string replacement of entities in build result > -------------------------------------------------- > > Key: CONTINUUM-1689 > URL: http://jira.codehaus.org/browse/CONTINUUM-1689 > Project: Continuum > Issue Type: Bug > Components: Web - UI > Affects Versions: 1.1, 1.3.4 > Reporter: Baptiste MATHUS > Priority: Minor > Fix For: 1.4.x > > > I just noticed that the output of maven is wrongly encoded. Here's one of lines of he generated html source : > [INFO] Setting property: resource.loader => 'classpath'. > Remind: In the console, the output is the following (output of mvn site) : > [INFO] Setting property: resource.loader => 'classpath'. > As you can see, there's a problem > I guess the entity conversion is done twice : > * first "=>" is converted "=>" > then another pass detects the & although it shouldn't process it anymore, so : > * second "=>" is in turn converted "=>" > I guess the correction weight might depend on how those conversion are done. As a lot of conversions produces an &, maybe encoding & as & should be the very first conversion to be done in the list ? > Maybe even some "dependency" tree could do the trick (or even better, an API that already does that neatly :)). > Cheers. > Let me know if you need anything else. -- This message is automatically generated by JIRA. - If you think it was sent incorrectly contact one of the administrators: http://jira.codehaus.org/secure/Administrators.jspa - For more information on JIRA, see: http://www.atlassian.com/software/jira
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I'm gonna fulfill my promise in last entry, blog about the BBQ party on last Sunday.
We'd a BBQ party is because we are replacing the exact Mother's day celebration a week before. Momi was quite busy on the exact date for wesak day, it was coincidence that they both fall on the same week and Ting, the tomo claimed that we were too late celebrating Mother's day which is a week after mother's day. Innocent wey! LOL..
*dancing in the rainbow* lol too exaggerated I know!
As I mentioned on my last entry (or you didn't read at all nah~), we had Korean style BBQ, but the food we had prepared is very Malaysian, so let's call it a mix Korean and Malaysian. Emmmm..is mixture baby yo!
From pork to chicken to vegetables to sauce to sweet potatoes to salad to otak-otak (fish and squid paste with chilli) from Muar to fish to fresh prawn to countless food.
I mean we didn't finish up the foods at the end, it was too much until everyone surrender. *waving the white flag*
Or nothing will happen coz she doesn't read my blog at all. Ok! Safe!
He is so dam cute and keeps act cool while wearing that sun shade lol!
Ps: he was sis's precious you know. Sis loves him so much as compared to Jun Jie (CK). So bad loh. I'm everytime fair and square. Isn't?
Ps: his teeth grow so fast after dropping for months. Especially the rabbit tooth dropped last year, and it is now all grow so nicely already.
I was the master chef that day and everyone so in love with my pork. Should trade something with them while serving them like barter system..emmm..
Cannot stop laughing at what bro doing, he smashed the dried squid after BBQ, and becames fried squid flour. Lol
It was uncle and aunty idea to wrap vege with it because the previous two was too dry without vegetables, and this is perfectly cooked.
What I expect for kids at 8 years old. Haha!
Oranges didn't match well with the yogult at all.
The whole bowl of this disappeared in seconds. Everyone was like grabbing non-stop. I think this is faster than "whole sale" our BBQ chicken and pork.
Hotdog was another best sales of the year because those kids got to BBQ themselves for the first time! They were like keep grabbing the uncook hotdog to the stove and "burn" it lol. The only thing I heard from them was 姐,好了吗?(sis, is that okay?) 我要多多 honey (I want more honey!!)
So funny but I think is not bad thing after all, at least they got to try something new and willing to do it all by themselves. Feel free coz we didn't need to entertain them as they already self-entertained. Haha!
Ps: Lewis wasn't a great sparkingly juice opener, and this is a training for him to be my bro-in-law hahaha! All of us laughed when he finally open the thing and the thing still in his hand lol. ( sorry, doesn't know the name of the cover)
The little one laughed the loudest.
Anyway, we ended quite early that day, around 9 or 10pm and all of us cleaned up the place quickly with the help of JS. Chit-chat for another hours just to catch up and praising Lucky girl the timid one.
She is really so clever that day, keep shaking hand with me hahaha! I think she is scared of so many people in my house. Nicky boy was the pity one as he needs to be lock up behind without light on, coz lots of insect that day. He was so scared of dark I should say, never ever off the light for him throughout 8 years you know.
Alright! Kinda hungry, and should grab something to eat now teehee


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A three-part series on faith, discipleship and witness from my northern Alberta garden.
My kids love fruit. Our local grocery store always has a basket of free fruit, offered to help keep kids "healthily" occupied while shopping. Choosing their respective "shopping" fruit is an adventure, and our first stop. They especially love the fruit that we can grow at home: raspberries, currants, sour cherries, strawberries, crab apples, pine berries, the occasional blueberry, gooseberries, rhubarb, goji berries, sweet tiny tomatoes (yes, tomatoes) and in particular, haskap, or more commonly known, honeyberries or blue honeysuckle. This year, we are attempting ground cherries, some new golden raspberries, valiant grapes (again!) and will be adding a dwarf apple tree to the front yard.
Growing fruit in northern Alberta is not without it's challenges. While there are many great fruits that can be grown with abandon (can you say giant rhubarb), there are others that simply cannot thrive in our growing conditions, and if they survive at all, they are stunted and do not produce. Our growing season is short. Our nights remain cool. Even our prolonged high temperatures do not remain consistent enough to sustain certain heat loving fruits. Bananas and mangoes will forever remain a grocery store purchase, though kiwi has become a distinct possibility! I have managed, in the past, to grow enormous mini-watermelon plants; their vines overtook the garden bed, the sidewalk and the fence. Yet they produced no fruit. They were an impulse purchase, to placate my four and a half year old, from a local greenhouse and I had no clue how to grow them. I spent little time preparing, and simply planted them in a sunny spot in the yard. If I had taken a moment to do a little research, I would have realized the perfect, warm and sunny location they needed for fruit production was next to my house, where the heat would settle and reflect and create a micro climate. So instead, they greedily soaked up much needed nutrients, ran roughshod over my feeble attempt at flowers, and did not produce fruit. They had potential, but they ultimately did not provide the fruit we were looking forward to.
Our discipleship efforts are much the same as my watermelon misadventure. Often we have high expectations, and yet fail to remember the commitment required. We forget our part is dependent on more than our dreams and wishes, our visions and desires. We live expectantly, waiting (impatiently) for the blossoming fruit, while neglecting the conditions required to obtain a harvest. In ministry, we desire to produce disciples … forgetting that growth and production is God's domain (or the respective plant's if you are following the metaphor) and we are instead called to cultivate disciples. Read more
We do not produce disciples, we cultivate them; discipleship is not the result of rote production, but exquisite cultivation.
In the back of my yard along the fence, grow raspberry bushes. They were transplanted from the huge patch at my in-laws. There must be something in the dirt out there at the ol' chicken farm, because these things are kind of mutant-like. Let me explain: they rooted and took off almost immediately, they produce an amazing amount of sweet and tangy berries, and they spread, via their roots, rapidly. By rapidly, I mean out of control – pure invisible chaos! They spread to the point of growing two feet out the other side of the fence; they spring up randomly in the compost pile and find a home right in the middle of the green beans! They have not yet reached the status of a bramble or thicket, but they are definitely edging closer.
When I think of discipleship, I think of my raspberry patch. Cultivating. Pruning. Experiencing. I cannot help but be reminded of Jesus' teaching in John 15 as I set about pruning the patch, hoping (and praying) that they will provide a bountiful harvest for my children to enjoy as they play in the yard. Each fall I cut the old canes to the ground, as they will no longer bear any fruit and tend to just get in the way. New canes emerge and grow quickly, sometimes in unwanted places! This year, the first order of business is some intense spring pruning, digging and relocating. Anyone need some raspberry plants?
Discipleship is messy. Discipleship is painful an prickly (raspberry thorns anyone?). Discipleship is hard. Discipleship will push you to the brink, and then some, which my raspberry plants may soon find out! Discipleship leads to thicker and fuller and deeper union and communion with God, through the Lord Jesus Christ and transformation by the Holy Spirit. Consider these words:
"I am the true grapevine, and my Father is the gardener. He cuts off every branch of mine that doesn't produce fruit, and he prunes the branches that do bear fruit so they will produce even more. You have already been pruned and purified by the message I have given you. Remain in me, and I will remain in you. For a branch cannot produce fruit if it is severed from the vine, and you cannot be fruitful unless you remain in me. Yes, I am the vine; you are the branches. Those who remain in me, and I in them, will produce much fruit. For apart from me you can do nothing. Anyone who does not remain in me is thrown away like a useless branch and withers. Such branches are gathered into a pile to be burned. But if you remain in me and my words remain in you, you may ask for anything you want, and it will be granted! When you produce much fruit, you are my true disciples. This brings great glory to my Father." (John 15:1-8)
If we do not produce disciples, but instead cultivate them; and if discipleship is indeed not the result of rote production, but exquisite cultivation, then we must begin with the source of life. If a plant is growing, it is alive. For a disciple to grow, they must be alive. Jesus tells us that to be alive is to be attached to Him. We have been pruned and purified by the message of Jesus Christ. He is the true vine, the one in whom we must remain, and when severed from reveals we can do nothing … we cannot grow and therefore are dead. Our responsibility is to remain connected to Jesus, the one who gives and sustains and produces. We cannot produce genuine growth and change; our hearts and character are shaped by the Holy Spirit. We can however cultivate the soil and the connection – our lives of faith. If we attempt to produce and grow apart from Christ, we will end up with enormous mini-watermelon plants: greedily soaking up the next best thing; running roughshod over those around us; and eventually withering away and rotting, without ever showing signs of that which we were made to do.
To cultivate and allow ourselves to be cultivated in discipleship is not easy; however, as we open ourselves up to the possibility, new life is infused into fallow ground. But it can be prickly. It can be dangerous. It can be more rewarding than we ever imagined. Sometimes, there are moments of explosive growth, in our lives and in our gardens, and then it tapers off, finding a rhythm before bursting forth with an abundance of fruit. Other times, the growth is slow and steady, responding to the pruning of the gardener, with ease and constancy. And there are times when growth must be restarted by a complete transplant; new soil, new location, intentional cultivation.
In this time of Lent, let us begin anew. The prophet Hosea provides us with timely wisdom: "Sow righteousness, reap love. It's time to till the ready earth, it's time to dig in with God" (Hosea 10:12a, The Message). What does that look like? Again, it will look different for different people, though there are some basic ways we can begin the process of cultivating and being cultivated.
One suggestion and encouragement here is to recall the words of Jesus in John 15.
Let us submit ourselves to the reminder of Jesus that God is the gardener of our lives. He is the one who prunes, effectively shaping and forming and disciplining His children, who are connected to the vine of life, Jesus Christ. The more the Lord prunes, the more fruit will be produced in the lives of those who call Him by name. When I leave my raspberry plants to their own agenda, they become an out-of-control mess. Damaged, diseased and dead. The dead canes are just that – dead. Uprooting a dead raspberry cane can be done with relative ease; they are severed from anything that sustains life. The damaged and diseased canes may still produce some fruit, but often the fruit is small and bitter. What areas of our lives are damaged, diseased or approaching death, in desperate need of pruning by the Gardner?
Perhaps, the simplest way to begin is to start breaking up any fallow ground in our hearts and our lives and invite the Lord to begin pruning, to His glory.


text: Search site: Submit Search About Shop Tour Instruments Fluke Ukulele Mandolin Flea Ukulele Firefly Banjo Cricket Violin Fluke SB Timber Bass Custom More Products Songbooks & Instructional Materials Strings Cases, Hangers, & Stands Electronics Hardware & Fun Stuff More Instruments Jewelry & Apparel Gallery AT FireFly Custom DS 1 Custom DS 2 Custom Dye Sublimation Custom ER 1 Custom Gecko LE EJD Fire and Ice Giant Uke Lauren and her new Fluke Photographic dye-sublimation Woodie Amethyst Honu Fluke Aussie Fish Fluke Avocado Flea Custom dye-sub printed top Custom Laser Engraved Peace, Love, Ukulele Custom Purple Cricket Firefly strut personalized Koa Paddle Black Cricket Anchor, custom Neck engraving Player Sound Port Player Sound Port - option Wedding Flea, personalized Blue Cricket Violin Custom painted elephant flea Sheffield Wednesday Football Club Logo Flag of Wales Flea Octopus Laser-cut Sound hole Flea Fleamingo Fluke Tattoo Schoenhut Flea Union Jack Fluke Charlie Brown Flea Engraved Firefly strut Tiki King Fluke Ukulele Rag Flea Artwork by Tyla Customer painted Fluke Pirate Flea Elvis Chicken Ian Stone Giant Ukulele Tassel Doll Band Kleinette Firefly Dealers Libraries Press Video Clips Testimonials Support FAQs Fluke/Flea Ukulele support Cricket Violin support Firefly Banjo support Timber Bass support Instrument Specifications Shipping Info Warranty Returns Contact Nav Menu 2 Nav Menu 3 Nav Menu 4 Nav Menu 5 Nav Menu 6 Nav Menu 7 Nav Menu 8 Magic Fluke MENU Open menu Toggle search bar CART ( ) Search... Submit search Home > Instruments > Flea Ukulele > Koa Flea Ukulele other views: Koa is the tropical hardwood of choice for many traditional luthiers. Our solid koa soundboards provide a more lively and bright acoustic sound, as well as a beautiful figured grain aesthetic. Solid koa soundboard with hardwood fret board, solid walnut neck, bubinga bridge and adjustable saddle. See gig-bag and case options below. price: $364.00 Flea Scale Length choice Soprano 14" : 14 Fret Concert 15.5" : 15 Fret [+$14.00] Peghed Tuner Upgrade Upgrade to Peghed internally geared tuners [+$75.00] Electronics K&K: K&K Soundboard Transducer [+$75.00] K&K Aloha Twin: K&K Aloha Twin pickup designed exclusively for Ukulele [+$110.00] Mi-Si Preamp: Mi-Si battery-free pre-amp [+$149.00] Fret Markers Side Fret Markers: Fret Markers [+$15.00] Player Sound Port Player Sound Port [+$25.00] Grip Strips Grip Strips (professionally preinstalled) [+$4.00] Hardshell Case Type Flea/Firefly [+$95.00] Softshell Gig Bag Ukes Gig Bag for Ukes: Soft Shell Gig Bag Navy [+$49.00] Custom Notes Make notes here to start a dialogue with us about customizing your instrument. Accessories Clip-on Ukulele Electronic Tuner Strap for Fluke or Flea Hardshell Case Flea/Firefly price: $15.00 Add price: $15.00 Add price: $95.00 Add Average Rating: 5 of 5 Total Reviews: 1 Write a review » 1 of 1 people found the following review helpful: Cannot go wrong December 23, 2015 Reviewer: Chris W. from Victoria, BC My daughter needed a ukulele for music class at school, so I ordered a soprano Flea for her and, while I was at it, a concert Fluke for me - natural finish, no fancy options. Both are superb instruments. Intonation, action, fit and finish... everything is perfect.The smaller body of the Flea suits my daughter well, but I enjoy the bigger, more resonant tone of the Fluke - the difference is definitely noticeable. I have put some Worth strings on the Fluke (clear, hard, low-g) and they sound absolutely fantastic. The only bad thing I have to say about the Fluke is that now my Firefly isn't getting all the attention it used to. 8^) Was this review helpful to you?


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This explains the situations that are outlined in the Act where you do not need a WWC Check even when you are engaged in child-related work.
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|Working with Children Check Unit, Department of Justice
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|State of Victoria, 2018.
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Working with Children Check Unit
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Customer Support Line: 1300 652 879
Hours of operation are 8:30am - 5pm weekdays (excluding public holidays)
If you need an interpreter please call the Translating and Interpreting Service on 13 1450 and have them contact the Working with Children Check Customer Support Line on 1300 652 879.
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That's why whenever you spend your hard earned money with us we think it's only polite to say thank you for choosing GetGeared by rewarding you with G points to spend in the future.
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We work closely with the Royal Mail and the main regional courier company for your country to ensure that your goods get to you reliably. Each delivery charge applies to the whole order: An order can contain several items and delivery will still be the same low cost.
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Indira Gandhi National Open University will be admitting students for the Master of Education (M.Ed) programmes starting from January, 2014 through an Entrance Test all over the country on Sunday 8th September 2013
IGNOU M.ED Admit Card 2013 will be available before 10 days on official website of IGNOU. The last date to apply for the Master of Education (M.Ed.) application form is 8th August 2013
M.ED Eligibility
B.Ed. with fifty five percent marks. Two years teaching/professional experinece after completion of B.Ed programme in a Government/Government recognized school/NCTE recognized teacher education/research institution in education.
The reservation for SC/ST/OBC (non-creamy layer) and other categories shall be as per the rules of the Central Government/State Government, whichever is applicable.
i) The reservation for SC/ST/OBC (Non-Creamy Layer) and other categories shall be as per the rules of the Central Government.
ii) The candidate should submit their application form only at concerned Regional Centre under whose jurisdiction/operational area the workin school falls.
iii) Masters' Degree awarded without a first degree is not accepted for purpose of Academic Studies in IGNOU.
iv) As per Government of India Notification dated 7th March, 2013, the concession for the wards of Kashmiri migrants for admission during academic session 2013-14 shall be adhered to with regard to the following:
1) Relaxation in cut off percentage upto 10% subject to minimum eligibility requirement.
2) Increase in intake capacity upto 5% course wise.
3) Reservation of at least one seat in merit quota in technical/professional institutions.
4) Waiving of domicile requirements.
PRICE : Rs. 1000.00 by cash at the Counter
Rs. 1050.00 by registered post
Candidate can download IGNOU M.Ed. Prospectus for the January 2014 session at http://ignou.ac.in/userfiles/Prospectus%20MED%20January%202014.pdf
For details, students can contact the Student Service Centre, Maidan Garhi, New Delhi at 011-29535714, 29533869/70, 29572512/13/14, email: email@example.com or the Regional Centres.


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The Love Of Our Lord
Listening to Punk Goes Pop: Volume Two this morning. Steaming it from imeem.com feeling pretty god about the mix so far. There a re few I could do without but there always are. Reflecting this morning on the love of Jesus and specifically how to love like Him. How to cary His example of love over into my life. In John 15 Jesus says in v.12 "This is my command, love each other as I have loved you."(NCV) It's not an option. It's a command. Jesus then continues with what 'the greatest love a person can show is to die for his friends." v.13 Wow. Jesus did this ... he literally fleshed this out. His flesh and bone has town from his body during his scourging before the cross so much that he was unrecognizable. The man of sorrows found in Isaiah. Jesus thank you for the cross. Thank you for the cost you paid no greater love have you ever showed to me than to be nailed to the tree. There is no one like you God.
Posted by Micah Andrew Hasty at 9:18 AM
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Dermatoglyphics – The discerning of Multiple Intelligences based on fingerprinting.
- The belief that you can infer intelligences from fingerprints is unfounded.
- There is no reason to think that fingerprints would be yoked to a specific intelligence or the theory of multiple intelligences.
- Knowledge of one's intelligences does not dictate life trajectory. We should not predict someone's career choices based on their intelligences, let alone on the basis of their 'fingerprints.'


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Dymatize's Mega Gainer - Product Review
Dymatize's Mega Gainer features a NCAA compliant weight gainer specially formulated for strength athletes, body builders and football players. It provides the perfect ratio of essential and non essential amino acids with complex and simple carbohydrates. Mega Gainer delivers a complete source of extra quality calories to promote muscle mass and weight gain.
Mega Gainer's Megatein blend contains whey protein concentrate, non fat dry milk powder, whey protein isolate and ultra micro filtered whey protein. The enzymatically predigested proteins support protein synthesis and positive nitrogen retention (stored muscle protein) for faster muscle gain and recovery. The addition of BCAA's (branched chain amino acids) leucine, valine and isoleucine and conditionally essential amino acid glutamine help prevent muscle tissue breakdown and support muscle growth and repair.
Mega Gainer's maltodextrin complex carbs help preserve liver and muscle glycogen (stored energy) levels. This decreases muscle fatigue and extends endurance and performance. Maltodextrins help prevent muscle breakdown after workouts and support nitrogen retention (stored muscle protein).
Mega Gainer is NCAA compliant and contains over 21,400 mg BCAA (per 100 gm protein) and 17,100 mg glutamine (per 100 gm protein). It is instantized, for instant mixability.
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People who are allergic to dairy products should avoid whey protein. Few side effects are associated with glutamine supplementation. However, mega-dosing with glutamine over an extended period of time may result in potential side effects such as diarrhea, weakness and nausea.
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If you're looking for a NCAA compliant weight gainer, Mega Gainer is for you.
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Dosage and Directions


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What is CAD
Drawings created with Computer-Aided Design (CAD) tools, which were introduced in the 1960s, represented tremendous productivity gains over paper drawings, such as ease to revise and archive. CAD tools also opened new opportunities, such as enabling manufacturing instructions to be derived automatically and executed directly from the drawing. Nevertheless, as computer design and manufacturing tools proliferated to meet increasingly complex and diverse engineering needs, so did the formats that each tool used to capture and store product data. While paper drawings can be marked up by anyone with a pencil, a product model that cannot be interpreted by the necessary CAD tool is useless. For organizations to share designs across various CAD and Computer-Aided Manufacturing (CAM) tools, their data files must be formatted in a manner that the tool can recognize. This requirement has become increasingly important in an age where large manufacturers often form joint ventures to address a business opportunity, and where partners in a supply chain are being called upon to deliver an increasingly complex array of services.


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The likelihood of having depression is significantly increased in people with deficient level of vitamin D, compared with people with adequate levels of the sunshine vitamin, says a new study.
Data from the third US National Health and Nutrition Examination Survey revealed that people with vitamin D deficiency were at a 85 percent increased risk of having current depressive episodes, compared with people with sufficient levels, according to findings published in the International Archives of Medicine .
"It is not known, whether vitamin D deficiency leads to the depression or depression leads to the vitamin D deficiency," write the researchers from Georgia State University. "Further studies are needed in deciphering the precise role of vitamin D in psychosomatic disorders.
"Although the direction of the cause and effect relation between depression and vitamin D deficiency is not known clearly at this time, in public health perspective, the coexistence of vitamin D and depression in the US population at large is a concern," they add.
"It is important to identify persons who are at risk for vitamin D deficiency and/or for depression and to intervene early because these two conditions have enormous negative consequences on long term health."
D and depression
Vitamin D is somewhat of an unusual "vitamin," because it can be made in the body from sunlight and most foods do not contain vitamin D unless added by fortification. Synthesis of vitamin D in the body requires exposure to ultraviolet light and can be influenced by genetics, skin color, and sun exposure.
Reports of greater than expected vitamin D insufficiency coupled with emerging evidence that higher circulating concentrations of this nutrient may protect against cardiovascular disease have prompted a renewed interest in teasing out how environment, genetics, and behavior work independently and coordinately to influence vitamin D status. To help clarify this, researchers at Emory University studied vitamin D status in twins living in different North American locations.
"The results of the Karohl study are quite important," according to American Society for Nutrition Spokesperson Shelley McGuire, PhD. "Over the past couple decades, nutrition scientists have discovered that maintaining optimal vitamin D status is important for much more than keeping our bones strong. It's also critical for keeping our immune systems healthy and may help protect against diseases like heart disease and cancer. This study suggests that, whereas genetic differences impact winter vitamin D status, lifestyle choices and sun exposure (factors we can control) are predominant in the summer months. Additional research is still needed in more heterogeneous populations."
The authors concluded that during the winter vitamin D status is governed mainly by genetic factors. Conversely, non-genetic factors are most important during the summer. Future studies designed to better understand what these factors are will be especially useful as public health experts continue to explore ways to increase vitamin D status in different populations living under varying environmental and dietary situations.
And the World Health Organization (WHO) forecasts that within 20 years more people will be affected by depression than any other health problem; it ranks depression as the leading cause of disability worldwide, with around 120 million people affected.
This is not the first time that vitamin D has been linked to symptoms of depression. Dutch scientists reported in 2008 in the Archives of General Psychiatry that low levels of the vitamin and higher blood levels of the parathyroid hormone (PTH) were associated with higher rates of depression among 1,282 community residents aged between 65 and 95.
Furthermore, a review by Bruce Ames and Joyce McCann from the Children's Hospital and Research Center Oakland highlighted the role of the vitamin in maintaining brain health, noting the wide distribution of vitamin D receptors throughout the brain.
According to the review (FASEB Journal, Vol.22, pp. 982-1001), the vitamin has been reported to affect proteins in the brain known to be directly involved in learning and memory, motor control, and possibly even maternal and social behaviour. Depression in the elderly is highly prevalent and can increase the risk of medical illnesses, worsen the outcome of other medical illnesses, and may increase mortality.
New data
Vijay Ganji Ph.D., R.D and his Georgia State co-workers analysed data from 7,970 US residents aged between 15and 39. Assessments of depression were performed using the National Institute of Mental Health's Diagnostic Interview Schedule.
Results showed that people with blood levels of vitamin D of 50 nanomoles per liter or less were at an 85 percent increased risk of having current depressive episodes in persons, compared with people blood levels of at least 75 nanomoles per liter.
"The mechanism through which vitamin D plays a role in metal health is not clearly understood," said the researchers. "Active vitamin D enhances glutathione metabolism in neurons, therefore, promotes antioxidant activities that protect them from oxidative degenerative processes."
The researchers also not that vitamin D is involved in gene expressions for the production of neurotransmitters like dopamine.
The researchers stress however that their results do not prove that vitamin D deficiency causes depression, and called for additional studies to decipher the association between vitamin D and depression.
Source: International Archives of Medicine


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At a recent lymphedema clinic appointment, the medical staff were impressed with the fact that Sarah has completely reversed her lymphedema. Since Sarah had her lumpectomy and removal of the lymph nodes under her left arm, it is a condition to which she is now susceptible.
Her treatment, which included the aforementioned operations, four months of chemotherapy and three weeks of radiotherapy ended in February 2014. Later in the year she had some lymphedema swelling in her left arm, which measured 8.5% bigger in circumference than her right arm. We had previously bought a multi-gym which is set up in the spare room to help her build up her strength after the chemotherapy. Because of the lymphedema we bought a vibro-plate – a stand-on machine that vibrates your whole body. The vibrations are known to help the lymph fluid to drain out of the swollen areas. "I'm just going upstairs for a wobble," she tells me.
In October 2014 we took up 1950s-style dancing, including jive, lindy hop and swing dance. In January 2015, she took control of the reins in the kitchen (I have problems with portion control) and decided to embark upon a new healthy-eating lifestyle, cutting out alcohol, sugar, carbohydrates and processed foods. After ten months, she had lost three stone and is now down to a more or less ideal weight. We dance two or three times per week, which keeps her arms on the move. At the lymphedema clinic appointment, they found that her lymphedema had completely reversed – her left arm having reduced in size from 8.5% bigger to 6.5% smaller than her right arm! She was told that such a recovery was rare. Someone told me that they didn't realise that this was even possible.
I have no idea what all the fuss is about, yet all this fuss is being made. Could the combination of weight loss, proper clean–eating, the vibro-plate, sugar and alcohol abstinence have contributed to the unlikely (bordering on impossible, apparently) reversal of her lymphedema?
I'd love to hear from people who know more about this than I do.
Sarah's stylish lindy hop 50th birthday dance, December 2015:
Visit our website: http://www.markdpritchard.co.uk


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Mages are the undisputable kings of burst damage. They have the worst armour and generally very low hitpoints, so rely on killing quickly. They also have a range of abilities which prevent damage by preventing their opponent from attacking wow gold. There are two major PvP builds – the frost mage and the fire mage.
The frost mage is a high-survivability, moderate damage build. There are a number of tricks which allows the frost mage to survive combat for a much longer period than other mage builds wow gold, which is important because of the low base survivability that mages have.
[read the review on Story Behind Varus Retribution League Legends]
This survivability comes from too major areas: talents which unlock abilities that other mages don't have access to, and kiting. The two key surviving abilities that frost mages have are Ice Barrier and Ice Block. Ice Barrier absorbs a set amount of damage rip dvd, and is on a 30 second cooldown (it lasts one minute, which means that if you time your application correctly, you can re-apply it immediately). Ice Block is basically immunity, but with the detriment of preventing any action from the mage.
When using Ice Block, it is important to remember that you can remove the buff yourself whenever you want. You don't have to wait the entire duration – if you right click on the buff, it will instantly remove it, and you are free to make any action convert video. This makes it excellent for removing debuffs and returning to combat, or waiting temporarily for backup. It also confuses the opposition if you break early. It's usually best to Blink as soon as you remove the buff.
Other than those two abilities, Frost Mages are excellent kiters. This is the process of killing your opponent while preventing them from attacking you blu ray ripper, through slowing spells and use of Blink/running. Cone of Cold, which has a long snare duration, and is insta-cast, is a key to effective snaring, and Frostbolt will be the majority of you damage in a 1v1 situation.
Frost mages are also great in group PvP, especially if they have Improved Blizzard. In AV, they can slow an entire push very effectively for a low mana cost blu ray ripper. The key to this is to not cast the max rank Blizzard (as most people aren't stupid enough to stay in the AoE for the entire duration), but rather to cast Rank 1, as it still contains the slow effect (assuming you have the talent for it), and costs much less mana.
The fire mage has a greater capability for burst damage, but lower survivability than a frost mage. Fireball does greater damage than Frostbolt wedding gowns, and Ignite makes up a large portion of DpS which frost builds lack.
The key to fire mage success is gear and Presence of Mind. Damage boosting trinkets, like the Zandalian Hero Charm, provide a massive boost to power Wedding gowns, and should be used in combination with Fireball/Pyroblast and Presence of Mind. Because of the long cast time of Fireball (and to a greater extent, Pyroblast), it is rare that you will have the opportunity to cast without someone noticing you and attempting to interrupt your spells. visit glasskaar for more…


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Many homeowners start the new year with a long list of home-improvement projects. Since your house is often your greatest investment, it's imperative that you invest in maintenance projects to preserve the value of your home. Replacing your windows is a simple change that has a big impact. With ...Read MoreRead More


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A game show buzzer.
A bee flying around the room.
A fella named Buzz who likes his burgers.
A chainsaw getting ready for work.
A little boy getting his haircut for the first time with electric clippers.
Someone calls kissing bussing but this time used a Z to give it more bite.
The sound of a crowd murmuring with anticipation.
A vibrator.
A new washing machine that makes a noise to let you know it's done.
A cell phone set with a buzzing ringtone.
A faulty electrical outlet that sparks every time you plug something in.
Electroshock therapy.
The memory they implanted that mouse with.
The button that somebody got for Jacob that got pushed whenever he was being an asshole. It got pushed a lot and nobody enjoyed it more than Jacob. Jonathan eventually ran over it with the van.
Dragonflies' wings sort of buzz too – when they fly real fast and right past you.
Buzz, buzz.
A game show buzzer.


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Experimental evolution of aging, growth, and reproduction in fruitflies
Edited by May B. Berenbaum, University of Illinois at Urbana-Champaign, Urbana, IL, and approved January 24, 2000 (received for review July 12, 1999)
We report in this paper an evolutionary experiment on Drosophila that tested life-history theory and the evolutionary theory of aging. As theory predicts, higher extrinsic mortality rates did lead to the evolution of higher intrinsic mortality rates, to shorter lifespans, and to decreased age and size at eclosion; peak fecundity also shifted earlier in life. These results confirm the key role of extrinsic mortality rates in the evolution of growth, maturation, reproduction, and aging, and they do so with a selection regime that maintained selection on fertility throughout life while holding population densities constant.
In this paper, we report a case study in experimental evolution with the fruit fly Drosophila melanogaster that is designed to test predictions of life-history theory (1–6) and the evolutionary theory of aging (7–11). It did confirm those predictions. The change in the environment that drove phenotypic change was a difference in extrinsic adult mortality rates. What is different in this experiment is that mortality was applied in a way closely resembling natural conditions rather than by using traditional artificial selection. Treatments differed only in adult mortality applied twice each week, which maintained selection on fertility throughout life.
Evolutionary Theory of Aging and Life Histories.
Evolutionary theory predicts the impact of a difference in extrinsic mortality on intrinsic mortality rates (hence, lifespan) and on growth, maturation, body size, and reproduction. When extrinsic mortality rates increase, they lower the probability of survival to a given age and cause the strength of selection to decline faster with age, making an increase in intrinsic mortality rates with age "more affordable" or "less avoidable." From this concept follows a central prediction of the evolutionary theory of aging (11): Higher extrinsic mortality rates should lead to higher intrinsic mortality rates and a decrease in lifespan, which is a prediction adumbrated by Weismann (7) and Medawar (8), explicit in the work of Williams (9), quantitative in the research of Hamilton (10) and Charlesworth (1), and consistent with comparative evidence (11–13).
Extrinsic mortality rates also affect the evolution of other life-history traits. Higher extrinsic adult mortality rates should lead to higher reproductive effort early in life and, for age- but not stage-dependent life histories (5), to more rapid development and eclosion at an earlier age and a smaller size (1–6).
Experimental Evolution and Artificial Selection.
Recently, a new tool has been exploited to test such predictions: experimental evolution (14). In contrast to artificial selection, in which the experimenter determines which trait is selected, in experimental evolution, the experimenter creates the conditions under which a prediction should hold and lets the evolving population determine with which traits the problem will be solved. This approach has yielded important insights with bacteria (14–16), algae (17), poeciliid fish (18), and temperature adaptation in flies (19–22). It succeeds because evolution is more rapid than until recently was expected (23, 24).
Previous artificial selection experiments on Drosophila (25–31) suggested that the evolution of lifespan is constrained by genetic links between early-life traits, such as early fecundity and larval competitive ability, and late-life mortality and fecundity rates. The longest running artificial selection experiment on the evolution of aging in fruit flies (25) started in 1976. One set of lines is only allowed to reproduce early in life, another only late in life; fecundity and survival are the targets of selection, and the evolutionary responses in traits such as intrinsic mortality are measured. The late-reproducing lines have evolved a much longer lifespan and lower intrinsic mortality rates than the early-reproducing lines. This experiment has been repeated in another laboratory with a design that avoids unintentional selection and controls larval density (31). In this second case, the late-reproducing lines have improved survival and decreased early-life fertility, but there has been no improvement in late-life fertility, and no correlated responses to selection in the preadult period have been observed. In the best field test of life-history theory, still continuing in Trinidad (18, 24), extrinsic mortality rates of guppies are manipulated by exposing them to different predators. Significant genetically based changes in age at maturity and fecundity in the predicted direction have been observed, and changes in lifespan are expected.
Our Experiment: High Versus Low Adult Mortality.
Our experiment subjected a stock of wild D. melanogaster to two treatments: high adult mortality (HAM) and low adult mortality (LAM). In contrast to previous experiments, we knew age-specific extrinsic mortality precisely, because we imposed it twice weekly. Thus, the problem posed by selection to the organisms differed from the problems posed by previous work (25, 31), because the LAM flies were contributing offspring to future generations throughout their lives, not just at the ends of their lives. This selection regime, which maintains selection on fertility throughout adult life, is a much closer approximation of the kind of selection that is likely to be found in nature than is selecting for flies that lay eggs only very early or very late in life (25, 31). Moreover, because the population density of both the adults (200 per population cage) and the larvae (250 per unit of larval medium) was the same in both treatments, the responses can be attributed solely to differences in mortality rates with no confounding effects of larval or adult density.
We checked that the predictions of both the evolutionary theory of aging and the reproductive-effort model held for our experimental conditions by analyzing computer models simulating the evolutionary dynamics of phenotypes endowed with different life-history traits and age schedules of intrinsic mortalities. In the simulations, these phenotypes were subjected to selection regimes with different adult mortalities that corresponded to the regimes used in the experiment. The models assumed the standard constraining relations among development time, body size, and fecundity that are known to Drosophila researchers (5). The prediction that the HAM treatment should evolve higher intrinsic mortalities held for a range of assumptions about the shapes of intrinsic-mortality curves, including Gompertz (32) and sigmoid. The predictions of the reproductive-effort model also were confirmed. The computer models predicted that HAM flies would evolve shorter development times, smaller size at eclosion, higher fecundity early in life, and lower fecundity later in life than did LAM flies.
These expectations have the following intuitive explanation. In this experiment, extrinsic adult mortality starts when flies enter a population cage at the age of 14 days from egg. Under the high mortality in the HAM treatment, most flies can only reproduce for a few days before they are killed; they should evolve high fecundity at the age of 14 days by eclosing early and by being at the peak of their age-fecundity curve when they enter the cage. In the LAM treatment, most flies can reproduce for several weeks; fecundity late in life is important and can be increased by eclosing later at a larger size, which, however, delays maturity and lowers fecundity at age 14 days. Note that if the shift in mortality rates occurs precisely at eclosion, rather than at a particular age, then an increase in adult mortality selects for a longer juvenile period (at low risk) and a larger size with higher fecundity at eclosion.
The founder stock of 820 flies consisted of 10 virgin males and 10 virgin females from each of 41 isofemale lines that were collected in and near Basel, held as isofemale lines for several years, then bred together in a single cage for about five generations to yield a genetically variable population. To found the selection lines, we collected eggs once a week for 5 weeks to get five age classes and to avoid the cycles of reproduction and age classes that persist in populations founded with flies of one age. The experiment started November 15, 1993, is continuing, and is maintained on a day–night cycle of 12 h of light, 25°C/12 h of dark, 20°C, and at 70% relative humidity. Eggs for recruits are collected from the population cages on Tuesdays and Fridays, at which time two bottles for each line are established, each with 250 eggs in a fixed amount of larval medium. The adults that hatch from these eggs are used 14 days later to replace flies that die or are killed in the population cages.
Each treatment consists of three replicates, and each replicate has 100 male and 100 female adults, enough to avoid inbreeding. Adult mortality rates are imposed and densities are reestablished by hand with 14-day-old flies on Mondays and Thursdays (Fig. 1); larvae are maintained at the same density in the same medium for both treatments. We could maintain constant larval densities because of excess production of eggs and constant adult densities, because we reared more flies than were needed. Extrinsic mortality was adjusted so that intrinsic plus extrinsic mortality reached the target level.
The mortality rates imposed and the rearing conditions have changed twice since the start of the experiment. In the first 13.5 months in the HAM treatment, 90% of the flies in the cage were killed and replaced twice per week; the probability of surviving 1 week as an adult was P = 0.01. In the LAM treatment, the probability of surviving 1 week as an adult was P = 0.64. In January 1995, larval density was raised from 6.25 to 10.40 larvae per ml, and food quality was lowered from 1% to 0.75% yeast in the larval medium. In January 1996, larval density was raised to 12.50 larvae per ml, and the adult mortality rate in the LAM treatment was lowered, raising the probability of surviving 1 week as an adult to P = 0.81. Those changes increased both the physiological stress on the larvae and the difference between the two mortality regimes. We have achieved the target adult mortality rates in the two treatments (Fig. 1).
Traits were assayed in vials, not in the population cages themselves, in measurements made parallel to the main selection experiment. For each of the three replicates in both selection regimes, 4 × ≈50 14-day-old flies (age measured from egg) were collected in 250-ml glasses and fed fresh yeast for 4 h, then allowed to lay eggs into Petri dishes for 3 h. About 26 h after egg laying, 10 vials with 25 larvae each were set up for each replicate. One day before eclosion began, the vials were positioned in random order in a machine that collected newly hatched flies at 2-h intervals. After eclosion (8–12 h) males and females were separated. Males were frozen, dried for 3 h at 50°C, and weighed to ± 1 μg. Females were weighed alive (±10 μg) and set up in individual vials (52 × 18 mm) with two males apiece of the same age and line to determine fecundity. The medium in the vial caps was covered with 1:10 (vol/vol) diluted fresh yeast; caps were changed every day. Eggs were counted automatically with imaging software until 95% of the females had died; dead and trapped males were replaced until all females had died.
Intrinsic mortalities were measured precisely in 1998, when the HAM treatment had experienced ≈90 and the LAM treatment ≈50 generations, on 5,000 flies of each sex for each replicate in two cages per replicate with 2,500 males and 2,500 females per cage (5,000 × 2 sexes × 3 replicates × 2 treatments = 60,000 flies). Dead flies were sexed, counted, and replaced every 3 days with white-eyed mutants to hold the density constant throughout the assay. At the end, the number of flies remaining in each cage was counted to estimate the number of escapees, which averaged about 4% of the flies. We distributed escapes uniformly over age classes in proportion to the number of flies surviving.
Fecundity, development time, and weight were analyzed by ANOVA [SAS Institute (Cary, NC) procedure GLM] with replicate lines nested within treatments and, where appropriate, replicate vials nested within lines. Line MS was used to test treatment effects. Mortality curves were analyzed with Cox regressions (SAS procedure PHREG) with the same design as the ANOVA. Data were right-censored for escapees.
Results and Discussion
The female flies responded in the first year of their new life in population cages by taking longer to develop, becoming smaller at eclosion, and decreasing slightly in early fecundity (male response was similar), regardless of the treatment. Time to eclosion increased in both treatments from 266 to 285 h. Dry weights at eclosion held constant at about 380 μg. Early (days 13–15) fecundity dropped from ≈75 eggs per day to ≈50 eggs per day (Fig. 2 a–c). The assay methods were improved during the first year of the experiment, and the changes in the absolute values of life-history traits during the first year can mostly be attributed to changes in the assay methods.
Divergence between the two treatments in time to eclosion and dry weight began after 1 year when larval food quality was lowered and larval density was increased; divergence in early fecundity began after 2 years when larval density was raised again and the adult mortality rate in the LAM treatment was lowered. In all three traits, divergence continued for 1–2 years, then slowed considerably or stopped (Fig. 2 d–f). The slow change at the beginning of the experiment can be attributed to two factors: the larvae were not food-stressed enough to express the genetic variation that allows a response to selection, and the difference in the adult mortality rates was not yet large enough to provoke a rapid response. After the stress on the larvae was increased in 1995 and again in 1996, and the difference in mortality rates was increased in 1996, the lines diverged rapidly, as predicted (Table 1). The HAM flies developed more rapidly, eclosed at a smaller size, and reached peak fecundity more rapidly. Then the dynamics slowed, either because the lines had reached new adaptive peaks or because genetic variation for improvements was exhausted by selection and inbreeding.
The results qualitatively confirmed the assumptions we made in the computer model: Flies that eclosed earlier were lighter (r = 0.86; P = 0.02) than flies that eclosed later and had higher early fecundity (day 13–15 after egg; r = 0.99; P < 0.0001, calculated with the six-line means).
Intrinsic mortality was significantly higher for HAM than for LAM flies from the 40th to the 75th day of life (Fig. 3). Thus, differences in extrinsic mortality rates did lead to the evolution of the expected differences in intrinsic mortality rates. Recently, there has been considerable discussion (33–36) about the shape of mortality curves, stimulated by the observation of decelerating mortalities late in life (37). In this study, female age-specific intrinsic mortality (dx) stopped increasing after about 65 days of age, then decreased, then increased again (Fig. 3), suggesting that mortality rates do slow their rate of increase and may decrease late in life.
We rarely have measured a significant difference between treatments in late fecundity in 5 years of yearly assays. In the HAM treatment, late fecundity is not under selection, but may be subject to reduction through mutation accumulation. In the LAM treatment, late fecundity is under direct selection in all survivors. The fact that late fecundity has not decreased more dramatically in the HAM flies suggests that the effects of mutation accumulation, if any, or of an antagonistic pleiotropic connection with early fecundity, if any, are not strong. Thus, our results do not support the existence of a genetic connection between early and late fecundity as suggested by Rose (25) but not by Partridge et al. (31).
Was there a connection between fecundity and subsequent mortality, a cost of reproduction? In 1996, we checked for a relationship between early fecundity and subsequent adult mortality by calculating the correlation of early fecundity with mortality rates defined on subsequent 5-day intervals (Fig. 4). With measurements on only six lines, power was low, but correlations were consistently strongly positive for about 2 weeks after the fecundity measurement and, for one age class, they were significantly so. Thus, we confirmed the positive relationship between early fecundity and subsequent mortality found by Rose (25) and Partridge et al. (31) that is a cornerstone of the life-history theory and the evolutionary theory of aging (5, 6, 11). However, in our case, the strength of the correlations arises because the HAM lines had (i) high early fecundity and (ii) high later mortality, whereas the LAM lines had (iii) low early fecundity and (iv) low later mortality, a pattern explicable as direct responses to selection by both traits in three (i, iii, iv) of four cases. Only the relatively high late mortality of the HAM flies (ii) is not selected directly and suggests either a link to early fecundity, decreased maintenance, or effects of mutation accumulation. Our results do not contradict stronger evidence from other experiments (e.g., ref. 38) in which the relationship measured could not have been caused by direct responses to selection.
This experiment tested and confirmed the prediction that higher extrinsic mortality rates lead to the evolution of higher intrinsic mortality rates, something that had been done previously (25–31), but never before with such a realistic and precisely measured difference in mortality regimes and with such precise control of both adult and juvenile population densities. It also confirmed all of the major predictions of life-history theory in a case in which mortality rates were known precisely and where the confounding effects of density variation in both adults and larvae were excluded. The interaction of extrinsic mortality rates with intrinsic constraining relations was sufficient to explain these results.
We thank Mark Drapeau, Dieter Ebert, Tad Kawecki, Nancy Knowlton, Tom Little, Linda Partridge, and three anonymous referees for improvements to the manuscript, and Anni Mislin, Hanni Zingerli, and Barbara Sykes for technical support. This work was supported by the Swiss National Science Foundation.
↵* To whom reprint requests should be addressed. E-mail: steve.stearns{at}unibas.ch.
This paper was submitted directly (Track II) to the PNAS office.
Article published online before print: Proc. Natl. Acad. Sci. USA, 10.1073/pnas.060289597.
Article and publication date are at www.pnas.org/cgi/doi/10.1073/pnas.060289597
- high adult mortality;
- low adult mortality
- Received July 12, 1999.
- Copyright © The National Academy of Sciences
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- Kaiser M
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