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Life Science
Learning Center
Life Science Innovations
Summer 2002
Summer 02 Proteomics
Proteomics: New Products for Protein Analysis
By Staci RohnSigma-Aldrich Corporation, St. Louis, MO, USA
The Human Genome Project, one of the most widely heralded projects in science, has provided a great deal of data about the location of genes and their sequence. However, the true biological function of these genes lies in the expression of their encoded proteins. The full range of proteins found in an organism is known as the "proteome" and the study of the proteome is known as "proteomics". Whereas DNA is composed of four nucleotides of similar structure, proteins are built from 20 different amino acids that differ greatly in size, charge, and structure. To complicate analysis, proteins are often modified following translation. These post-translational modifications include phosphorylation and glycosylation, though a number of other modifications exist as well. Thus, the complex nature of proteins makes proteomics a task of enormous proportions. Analysis of proteins starts with sample preparation, followed by protein separation, then characterization, and finally image analysis. Scientists who want to study proteins from start to finish must use multiple suppliers to obtain the necessary materials.
One of the companies quickly becoming a leading supplier in this field is Sigma-Aldrich. Sigma-Aldrich has taken enormous initiative and is making huge strides in the proteomics market thanks to years of experience in protein preparation and analysis, strategic alliances, and gritty determination. Working with companies like Proteome Systems™ (the company that coined the term "proteomics") in Australia and Shimadzu Biotech™ (formerly Kratos Analytical™) in the UK has allowed Sigma-Aldrich to offer products that simplify proteomics. Calling themselves "The Proteomics Alliance™," Sigma-Aldrich, Proteome Systems, and Shimadzu Biotech combined their talents in late 2000. The opportunity was enormous – a virtually unrivaled combination of reputation, resources, and expertise that could offer everything needed to run a comprehensive high-throughput proteomics facility. Reformulating and repackaging existing chemical products was the most obvious approach for Sigma-Aldrich. Working with the Alliance, they quickly released two lines of kits: one for sample preparation and one for the calibration of MALDI mass spectrometers. A multitude of other products has followed, each created to solve known problems in proteomics research.
Sample Preparation
The first step of proteomics research involves the isolation of proteins from a biological sample. Many proteins of interest are low abundance or membrane-associated, and therefore difficult to solubilize. Detergents, protease inhibitors, and reducing and alkylating reagents facilitate the protein solubilization and stabilization. However, standard detergents leave behind approximately 10% of the proteins, which poses an obvious problem for researchers studying low abundance proteins.
Higher protein loads, improved solubility, and increased visibility of low abundance proteins are the key benefits of Sigma's ProteoPrep™ sample preparation products. In addition, the ProteoPrep kit format provides pre-measured and pre-weighed products in individual vials, which allows researchers to obtain all the needed reagents at one time, with one product. It also significantly reduces the waste and time typically associated with reagent preparation. These kits are for specific or targeted protein extraction, and include new, innovative detergent formulations that significantly refine results on 2-D electrophoresis gels.
There are three ProteoPrep kits from which to choose, depending on the needs of the researcher. The Universal Extraction Kit (Product Code PROT-TWO) sequentially separates soluble cytoplasmic and membrane fractions. The Membrane Extraction Kit (Product Code PROT-MEM) allows total extraction of membrane-associated proteins, while the Sample Extraction Kit (Product Code PROT-TOT) provides multiple reagents for testing or optimizing extraction conditions for total protein extracts. The data below demonstrate the superior results achieved with the ProteoPrep Universal Extraction Kit in an E. coli extraction (Figure 1).
Isolation of specific organelles often aids in the study of targeted proteins and cellular processes. Intact mitochondria are often the target of protein sample preparation, particularly for researchers studying proteomics, or apoptosis and its relationship to diseases. An intact mitochondrial fraction can be obtained with the Mitochondria Isolation Kit (Product Code MITO-ISO1). This kit allows small-scale isolation of an enriched, functional mitochondrial fraction from both "hard" or "soft" animal tissue samples. Easy-to-follow protocol diagrams are included with an additional stain to determine the integrity of the inner mitochondrial membrane. The integrity of the outer mitochondrial membrane can be determined using the Cytochrome c Oxidase Kit (Product Code CYTOC-OX1). The Chloroplast Isolation Kit (Product Code CP-ISO) provides plant researchers a convenient procedure for the isolation of intact chloroplasts from plant leaves.
Protein Separation
The analytical separation of proteins is one of the most challenging steps of proteomics research. The key is finding the most efficient way to achieve accurate results. Sigma-Aldrich recognizes 2-D electrophoresis as the most reliable technique currently available to obtain rapid results, in large scale, and in parallel.
Reproducibility, accuracy, and comprehensiveness are the hallmarks of the Sigma-Aldrich electrophoresis system. The ProteoSilver™ Silver Stain Kit (Product Code PROT-SIL1) contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. The kit has been proven to have an extremely low background, as shown in (Figure 2) . ProteoSilver's detection limit of 0.1 ng/mm2 of protein (BSA) allows for superior detection of low abundance proteins and increased stability of the bands or spots. The MALDI-MS compatible ProteoSilver Plus™ Kit (Product Code PROT-SIL2) contains the same reagents found in ProteoSilver, with two additional reagents that help simplify the destaining required prior to MS characterization.
Protein Characterization
Multiple tools for protein characterization are available, but mass spectrometry is the most common method used by researchers today. Mass spectrometers are incredibly sensitive instruments that use molecular ionization to generate the mass-to-charge ratio of proteins and peptide fragments from protein digests, which leads to molecular weight determination and protein identification. Researchers may use the results to deduce theoretical information about the amino acid sequence of a protein. Over the past decade MALDI-TOF mass spectrometry has become easier to use, less expensive and more accurate. However, using poorly packaged, impure calibration reagents can reduce ionization efficiency and spectra. Again, Sigma-Aldrich offers assistance.
ProteoMass™ kits save significant time when calibrating MALDI instruments. Sigma's stringent quality control ensures an absence of impurities and contaminant interference by eliminating the possibility of Na+ and K+ leaching from packaging. Working with Shimadzu Biotech, the manufacturer of the Kratos Analytical AXIMA-CFR mass spectrometry system, Sigma-Aldrich has designed and tested the kits to ensure researchers using ProteoMass achieve accurate spectra and increased ionization efficiency. In addition, the kits offer multiple matrices, solvents, and standards. The standards can be mixed and matched to target a specific mass range. Each standard is packaged in multiple vials, which ensures fresh product is available, and eliminates the possibility of using old, opened products that may have degraded on the shelf. Three kits are available, each targeted to a specific mass range, and suitable for either external or internal calibration.
The spectra in (Figure 3) and (Figure 4) were obtained using the ProteoMass Peptide MALDI-MS Calibration Kit (Product Code MS-CAL2), for a mass range of standards between 757 Da and 3494 Da.
Image Analysis
This final step of proteomics involves analyzing and cataloging the identified protein structures. Bioinformatics has become increasingly important in this area. Proteome Systems rounds out the proteomics process with bioinformatic systems that efficiently generate data needed in protein analysis. In particular, the systems are geared to high-throughput proteomics in which the amount of data generated is far too extensive to be managed on an individual level.
While companies supplying the proteomics market have boundless opportunity to stake their claim, few have taken the initiative of Sigma-Aldrich. The optimized products now available provide proteomics researchers a comprehensive means to perform their research quickly, with products that have already been tested in leading high-throughput proteomics facilities worldwide. Using the full platform from the Proteomics Alliance, including equipment such as the Xcise™ or ElectrophoretIQ™ from Proteome Systems, and the Axima™ mass spectrometer from Shimadzu, means using systems and products that work together, backed by the expertise and support from pioneers in the field. The race to map the human proteome is underway, and Sigma-Aldrich has proven its commitment.
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65 Mitchell Blvd, San Rafael, CA 94903
phone: 415-453-9700 ext 102
· 2
November/December 2008
Currently, a combination of several factors has created a near perfect stormfor 2,4,6-TBAformation inwinery cellars: the presence of a wide variety of 2,4,6-TBP sources, the fact that the majority of wineries do not have cleaning and sanitation practices in place to completely prevent colonization of filamentous microbes on surfaces, and wineries´ need to maintain humid cellars (to prevent wine evaporation). As with 2,4,6-TCA, once 2,4,6-TBAis released into the atmosphere, it will cling to almost any surface, and once it gets above a certain threshold in the atmosphere, it will taint wine.
Sensory Threshold / Sensory Impact of 2,4,6-TBA
The sensory threshold of any chemical compound can vary, depending on the medium in which it is present (water or alcoholic solution) and/or on the individual conducting the sensory evaluation. This is also true for 2,4,6-TBA, which has a sensory threshold in water ranging from 0.008 to 0.03 ppt, and a threshold in wine ranging from 2 to 6 ppt. These sensory thresholds in water and wine are about the same for 2,4,6-TCA.
To give some perspective on the magnitude of such low concentrations, here are some analogies:
One ppt is equivalent to one cube of sugar dissolved in enough water to fill 100 Olympic-size swimming pools.
One ppt is equal to one second in 31,797 years.
One ppt is equivalent to 0.4 mm of the total distance from the Earth to the Moon (238,908 miles).
In wine, depending on the sensitivity of the individual, the presence of 2,4,6-TBA (or 2,4,6-TCA) will cause a loss of fruit intensity and aroma at lower concentrations (1 to 3 ppt) and an actual taint perception at higher concentrations (more than 3 ppt). At these higher concentrations, 2,4,6-TBA will impart musty, moldy, wet newspaper, wet cardboard, and wet cement odors and flavors to wine.
Preventive actions
To prevent haloanisole (2,4,6-TBA, or 2,4,6-TCA) taint in wineries, the following actions are recommended:
Perform periodic inspections (semi-annually or annually) to determine if a facility is free of 2,4,6-TBP, 2,4,6-TBA, 2,4,6-TCP, and 2,4,6-TCA, (plus other haloanisoles and halophenols not discussed in this column, such as 2,3,4,6-TeCP, 2,3,4,6-TeCA, PCP, and PCA). The periodic inspections should consist of testing atmosphere,water supply,water distribution system,wines (both cellared and those in marketplace), corks, wood material, paint, and plastic liners.While this is by nomeans a complete list, itwill provide an effective inspection of a facility.
Each winery should demand certification from manufacturers of incoming goods that their products are haloanisole- and halophenol-free including barrels, oak adjuncts, silicon bungs, fining agents, and shipping materials (wood pallets, cardboard, etc).
When new materials from "unapproved vendors" are introduced into winery (paint, plastic, or insulation), the material should be tested for haloanisoles and halophenols.
Conduct periodic cleaning and sanitation of the winery infrastructure (floors, walls, ceilings) to limit colonization of microbes on surfaces.
Eliminate the use of cleansers and sanitizers containing chlorine and bromine.
Due to the plethora of materials that can be origins of the 2,4,6-TBA precursor 2,4,6-TBP, and due to the fact that a very high percentage (nearly 80%) of all the filamentous fungi analyzed can produce haloanisoles, the probability of 2,4,6-TBAformation in a winery environment is very high. In addition, wineries must also contend with the actuality that 2,4,6-TBA can be brought into the facility from external sources (barrels, paints, wood materials, plastics, etc).
The ubiquitous nature of this chemical requires that wineries be extremely diligent about prevention activities. As the magnitude of the 2,4,6-TCA problem in the wine industry demonstrates, a mere reactive stance toward 2,4,6-TBA could lead wineries to dramatic economic andmarketing hardships.
Chatonnet, P., S. Bonnet, S. Boutou, and M-D. Labadie. 2004. "Identification and responsibility of 2,4,6-Tribromoanisole in musty, corked odors in wine." J. Agric. Food Chem. 52: 1255-1262.
Flodin, S., and K. J. Whitfield. 1999. "Biosynthesis of bromophenols in marine algae." Water Sci. Technol. 40: 53–58.
Patnaik, P., E. Powers, and J.N. Khoury. 2002. "Pathways of phenol-chlorine reactions in the presence of bromide ions in waste waters." American Laboratory News, Application Note. August: 26–28.
Coque, J. J.R., M.L. Alvarez-Rodrigues, and G. Larriba. 2003. "Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines." Appl. Environ. Microbiol. 69: 5089–5095.
Alvarez-Rodríguez, M. L., L. López-Ocaña, J.M. López- Coronado, E. Rodríguez, M. J. Martínez, G. Larriba, and J. J. Coque. 2002. "Cork taint of wines: Role of the filamentous fungi isolated from cork in the formation of 2,4,6-trichloroanisole by O-methylation of 2,4,6-trichlorophenol." Appl Environ. Microbiol. 68: 5860–5869.
Gribble, G.W. "The diversity of naturally occurring organobromine compounds." 1999 Chem. Soc. Rev. 28: 335–346.
Zoecklein, B. "Environmental taints, Enology Notes #94." Wine Enology Grape Chemistry Group, Virginia Tech, Virginia 2004.
Mara, P.A., and L. F. Bisson. "Bacterial causes of winery chloroanisole contamination." Papers and posters presented at the ASEV 56th Annual Meeting 22–24. June 2005, Seattle, WA.
[Dr. Robert Tracy, with nine years of scientific research experience and seven years of winery laboratory and quality control/- quality assurance experience, specializes in wine chemistry and microbiology, winery sanitation, and QA/QC. He is co-founder of BevTrac Mobile Quality Systems LLC, based in Windsor, CA (
), providing a mobile wine laboratory and consulting services to the wine industry. Please direct inquiries to firstname.lastname@example.com or tel: 707/239-8581.]
PG&E studies electrodialysis for cold stability
By Steve Fok, PE
From September/October 2008
Accumulation tables overcome unstable tapered bottles
By Ben Garvey
From July/August 2008
UC Berkeley introduces beneficial insects to control vine mealybugs
By J. Scott Gerien
From May/June 2008
Future of the wine industry - Climate Change Science
By Kimberly Nicholas Cahill, and Christopher B. Field
Stanford University and Carnegie Institution for Science
From March/April 2008
New fermentors for gentle handling
By Tina Vierra
From January/February 2008
Innovation for the winemaking toolbox
By Henry Work
From November/December 2007

subset: commoncrawl


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Submitted by ZX81 on Wed, 04/02/2009 - 8:49pm
I've uploaded the majority of the songs from my New Years set. Bear in mind they're unmastered as I was running the mastering live as a plugin - an they're missing keyboard/gated sounds which I played live :)
You can download them here.
- ZX81's blog
- Login to post comments


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Near $7.1M SAD 4 budget moved to May 15 vote at the polls
GUILFORD — A proposed $7,087,042 SAD 4 budget for the 2018-19 school year was approved — with all 20 articles being passed as written over about 20 minutes with nearly 50 registered voters present — during a district budget meeting on May 3 at Piscataquis Community Elementary School.
The near $7.1 million spending plan is now moved to a referendum in the SAD 4 towns of Abbot, Cambridge, Guilford, Parkman, Sangerville and Wellington on Tuesday, May 15 with residents of the half dozen communities voting either "yes" or "no" to make a district-wide decision on the 2019 budget. More information on the financials is available at www.sad4.org.
The $7,087,042 figure for 2018-19 represents an increase of just over $208,000 (3.024 percent) from the current year's budget of $6,879,013.
During an information session held before the district budget meeting, Interim Superintendent Raymond Freve explained the increase in the 2018-19 budget.
He said in addition to the $208,028 increase in the expenditure budget, revenue estimates are up $289,932. These increases have resulted in a rise in assessments for each of the six towns. The state's subsidy formula (ED 279) has dictated that SAD 4 will be raising more locally and be receiving less — a turnaround of about $202,699.
Freve said the district state valuation is up by $866,667 to $382 million for next year, the state mill rate will rise $0.32 to $8.51 and the projected total student count is 542 or 31 less than in 2017-18.
The superintendent said these three factors have resulted in a $73,665 state subsidy reduction (3.27 percent) to a total of $2,176,464 for the 2018-19 academic year. "To feed that number there has to be local increases in the budget," Freve said.
The local share of the spending plan totals $4,180,370, an increase of $290,375 from the 2017-18 figure of $3,889,995. The local share is made up of $3,235,991 in local required monies, just under $900,000 in local additional funds across the half dozen district communities and SAD 4's portion of the Piscataquis Valley Adult Education Cooperative expenses. The district is responsible for nearly $44,800 of the $428,555 adult education budget.
Each component of the $3,889,995 was passed via individual articles. The local required amount passed 39-5, the local additional funds were OKed at 42-7, and another 41-6 tally approved the $7,087,042 for the total 2018-19 budget.
Abbot's share of the proposed 2018-19 budget would be up by $49,117 to $793,972; the share for Cambridge is up $20,930 to $268,793; Guilford's share is up $112,480 to $1,393,403; the share for Parkman is up $52,918 to $654,967; Sangerville's share is up $38,219 to $858,086; and the share for Wellington is up $16,708 to $211,147.
The SAD 4 school board is scheduled to meet next on Wednesday, May 16 — the day after the referendum — at 7 p.m. at the elementary school.


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The goal of accreditation is to ensure that institutions of higher education meet acceptable levels of quality. Accreditation in the United States involves non-governmental entities as well as federal and state government agencies. Accreditation's quality assurance function is one of the three main elements of oversight governing the Higher Education Act's (HEA's) federal student aid programs. In order for students to receive federal student aid from the U.S. Department of Education (Department) for postsecondary study, the institution must be accredited by a "nationally recognized" accreditor (or, for certain vocational institutions, approved by a recognized state approval agency), be authorized by the state in which the institution is located, and receive approval from the Department through a program participation agreement.
UNLV's recent NWCCU Year 7 Comprehensive Evaluation took place in October 2017.
UNLV's self-evaluation report is available to read.
Accreditation Contacts
Gail Griffin
Questions or Comments
E-mail email@example.com with questions or comments.


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Manuscripts reflect a true and intimate side of LeRoy neiman
I have known for years and LeRoy Neiman , after his death his colleague Tara Zabor in LN studio in New York found that dozens of LN manuscript , never published before these manuscripts after his death I carefully studied these works , one of which Zhang is very appealing to me , the painting was created in 1949-1952 years, I've never seen this kind of style LeRoy creative works, but very compelling. So I think in the end , how many good works have never been published . These manuscripts reflect the dark side of LeRoy privacy .LeRoy Neiman such private manuscript reflects the creation of his real and intimate side, no extra but purely for their own purpose for such creations , so I made ??an ingenious proposition - how to reflect an artist 's manuscript real and intimate side, and how these translate into work to show to the public . If there are arts organizations will be able to realize this idea , it would be kind of situation ? Just a regular exhibition or those exposed to the public before the creation of private form of subversion is not the same ? And this in turn mean? These problems are waiting for us to find the answer , it is an interesting experience.
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Buch: Pattern Recognition and Signal Analysis in Medical Imaging (2. Ausgabe)
von Anke Meyer-Baese, Volker Schmid (Academic Press)
Medical imaging is one of the heaviest funded biomedical engineering research areas. The second edition of Pattern Recognition and Signal Analysis in Medical Imaging brings sharp focus to the development of integrated systems for use in the clinical sector, enabling both imaging and the automatic assessment of the resultant data.
Since the first edition, there has been tremendous development of new, powerful technologies for detecting, storing, transmitting, analyzing, and displaying medical images. Computer-aided analytical techniques, coupled with a continuing need to derive more information from medical images, has led to a growing application of digital processing techniques in cancer detection as well as elsewhere in medicine.
This book is an essential tool for students and professionals, compiling and explaining proven and cutting-edge methods in pattern recognition for medical imaging.
466 Seiten | ISBN: 9780124095458 (Hardcover), 9780128101162 (Taschenbuch), 9780124166158 (e-Book)


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It's time for a new Curtain Call Inspiration Challenge!
photo source: www.studiodiy.com
If you are a teacher like me, then maybe you see apples and primary colours when you look at the inspiration photo! LOL I sure did! We have one more month of school left and I have a few teacher thank you cards to make to thank the teachers who were mentors for me this year as I started teaching Grade One again after 14 years :) I couldn't wait to make my first apple shaker card with my PTI dies. Look closely at the confetti...they are tiny letters and numbers from the PTI pencil shaker die set. So sweet!
Make sure you visit each of the designers' blogs for more inspiration!
Beach Ball Designers
Michelle Ferguson, Guest Designer
Amy Kolling
Amy Sheffer
Amy Tsuruta
Gayatri Murali
Kim Singdahlsen
Laurie Willison
Lisa Henke
Marisa Ritzen
Maureen Plut
Monika Davis
Sarah Gough
Sheri Gilson
Stacey Schafer
Tenia Nelson
Tracey McNeely
Stop by the Curtain Call Inspiration Challenge: Beach Ball for the challenge details and to play along with us!


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White LadyMy name is Sara. Well to start off I'm not what you call a good girl so obviously I get into trouble.
I think it was 2 years ago I was at a 5th grade graduation party. So my mother calls me on the cell phone and told me to go home I scream at her no cause I was really enjoying my time so when I get home my dad pulls me into the room and starts beating the crap out me. So I feel like crap and I go to sleep It is about 1:00 am and I'[m really cold because the window was open and the white curtains were blowing and I had no blanket and when I open my eyes a little there is like a white figure and it is covering me with my blanket. It looked like a lady and she had long hair and she was wearing a long white dress with something white on her head and I felt comfortable so I fell asleep again. I WAS NOT DREAMING!! I saw it clearly.
So then in the morning I asked my mom if she did it and she said no. I knew it wasn't her because my mom is sort of chubby and she has blondish short hair. But I don't know if it was an angel or my dad's dead mother, which makes her my dead grandmother.


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"Venus in Fur" on the West End: It's Sexual and Violent (Like Game of Thrones!)
Wooot it's Theatre Thursday y'all! Today's review is of the new production of "Venus in Fur" in the West End, currently playing at the Theatre Royal Haymarket until December 9.
One of the Broadway shows I most regretted missing was the play "Venus in Fur", for which the joyous and adorable Nina Arianda won a Tony in 2012 for being the literal opposite of joyous and adorable. David Ives's play was generally referred to as a dark and twisted psychosexual…comedy? Drama? It is both, and it is neither. And that's really all I knew about it until I finally saw it last weekend, in the form of the new West End production starring Natalie Dormer (Game of Thrones, Tudors, you all love her) and David Oakes (whom I last saw in Shakespeare in Love, the West End play, as Marlowe). It's not the kind of show you enjoy while it's happening, but the kind where you can't stop trying to figure it out after the fact, which could be the more interesting option.
I <3 Diversions
Entertainment is a ridiculously big part of my life. I grew up in movie theatres, so in my family you have to see pretty much every movie to make it in conversation. This pop culture knowledge has extended to television, music, and, for me, the theatre. To quote Abed, I like liking things, so there's that.
February 2019
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introducing our chic Chip and Dip Set which is crafted from natural bamboo and features a non-porous anti-bacterial surface. It has high durability and is accompanied with a ceramic bowl.
Beautiful Home Bamboo Chip And Dip With Ceramic Bowl
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First time doing a research project? Not sure you have found the most appropriate sources for your paper, speech or presentation? Question too complicated to be addressed adequately by email or at a busy reference desk? Why not make an appointment with a City Tech librarian? We can help you:
- focus your research topic
- find relevant books, articles and websites
- develop effective search strategies
- evaluate information sources
To request an appointment, complete all parts of the form below. We will contact you by email within 2-3 business days to confirm the date and time of your appointment.
Research appointments are held at the City Tech library and are available to City Tech students only.
Questions or comments about research appointments? Contact Prof. Keith Muchowski at firstname.lastname@example.com or 718.260.5480.


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Kondgbia's response: "I didn't know Messi was playing in Madrid."
Kondgbia's response: "I didn't know Messi was playing in Madrid."
Puyol #5
Seems like I was right about that Kondogbia quote being fake.
Valencia CF wish to categorically deny non-existent statements falsely attributed to Kondogbia in reference to the footballer Leo Messi.
Looked like it originated from Mundo Deportivo lol
Having an outstanding season. Was great at Sevilla and Monaco too apparently. Inter must have been a really bad match for him.
Btw not saying we should sign him, but he definitely got some great qualities and seem to be getting back to a great level.
Last edited by DonAK; 9th February 2018 at 02:11 AM.
He's probably suited for Spanish football than Italian football IMO.
For the last couple of years I have been browsing through the Inter fan forums and he was always criticized for being slow in pretty much everything. Don't think he ever was a bad player, he just wasn't suited for Serie A.


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Need a quote? We'll be happy to provide information
for residential or commercial glass projects of any size.
Fill out the form below with the spacifics of your glass repair or project. Information like size, glass type, glass colour, and other idenifying details are all helpful in creating the most accurate quote.
Once the form is submitted a member of our team will contact you.


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Otago Polytechnic's Diploma in Ceramic Art (Level 6) is New Zealand's only specialised programme in ceramic arts at tertiary level. The part time course takes four years and can be studied at a range of centres around New Zealand.
Students study the theoretical aspects of ceramics online under the guidance of tutors at Otago Polytechnic. The papers are drawing, glaze technology and history of art. A studio practice paper is provided regionally by experienced professionals.
The DCA provides a great opportunity for those working with clay, or interested in ceramics, to enhance their skills levels and knowledge.
Please contact me if you have any questions about studying the Diploma in Ceramic Art right here in Otaki.


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Dog Training Quote of the Day…. How to pick a puppy!
The most confident puppy in a litter rarely makes the best pet, similarly the shiest puppy in the litter rarely makes the best pet, it's best to pick a puppy somewhere in the middle.
However, with proper socialization, training and lots of consistency, most any puppy can become a great pet. ~Mike Deathe, Founder of KISS Dog Training
886 Total Views 2 Views Today


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A website should have its own identity. It should not only look distinct but also be identifiable with its main purpose, activity, location, etc.
|Indian tricolour
|Tiger: Indian national animal
If you have a blog or website on nature photography, travel, Indian flora and fauna, etc, you can display tiger and peacock. Both make powerful visual elements. While tiger is majestic and forceful, peacock beats all animals with its fan of colourful feathers.
Travel, culture and nature sites can also display the Himalayas and the Taj. Culture, history and travel websites can use photos of Rajasthani folk dance, a classical dance, dancing Shiva, Ganesha, yoga, lotus and other cultural icons of India.
|The Taj Mahal
Avoid visuals showing poverty, icons of a particular religion, people – especially the poor, handicapped and socially backward - in bad light, superstitions, crime and filth. India has a good share of these all, but displaying them serves hardly any good purpose while it amplifies India's negatives at the cost of its strong cultural and social traditions.
This post is addressed specifically to Indian bloggers, but those from other nations can think of similar actions on their websites, blogs and other social media.
photo courtesy: India portal, Kerala Tourism, Wikipedia


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Andrew Wiggins Vs Julius Randle Part 2 Lives Up To The Hype! Overtime Thriller At Peach Jam!
Arek Kissoyan | | 17,468 Views | East
#23 of Mixtapes.
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Check out the highly anticipated re-match between 2 of the most dominant players in the nation, Andrew Wiggins (CIA Bounce) and Julius Randle (Texas Titans). The Titans lead for most of the game untill Xavier Rathan Mayes hit 4 three pointers in a row to bring CIA bounce back and eventually take the game into overtime. In overtime Julius Randle fouled out of the game with 3 minutes to go and Andrew Wiggins made sure to put the game away in this exciting overtime thriller. Andrew Wiggins finished the game with 28 points and 18 rebounds. Julius finished with 15 points, 13 rebounds and 4 assist.


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The current issue of PLOS One includes a recent paper by myself and my co-authors from UCSB which demonstrates that situations under which mind wandering is likely to occur are associated with increases in pupil diameter (see http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018298). These changes are distinct from the changes that take place when the mind processes external information. This study suggests that pupilometry maybe a useful covert marker for internal focus and provides further evidence that internally maintained thoughts (such as mind wandering or daydreaming) are often associated with a state of increased arousal (such as strong emotion or important personal concerns).
Mind-wandering is a product of spontaneous, internally generated thought
Recent thoughts
- Exploring the varieties of experience during the mind-wandering state using pupilometry
- Constraining the wandering mind: the role of control processes in deliberate mind-wandering
- Distant memories: Why the default mode network is important during states of internal focus
- Monitoring the mind: clues for a link between meta cognition and self generated thought
- Emotional wandering: considering the complex relationship between self-generated thought and happiness


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For the love of skin health & coconut oil
DIY Body care recipes for every skin type.
You know I love coconut oil for so many reasons, I am crazy for coconut oil is an understatement.
DIY body care:
Coconut oil is great for making DIY deodorant, DIY toothpaste & for DIY body care & hair care (& of course taking, oil pulling, cooking & baking but this post is about DIY body & skin care).
DIY Deodorant:
- 4 tbsp organic corn starch (or potato starch)
- 4 tbsp baking soda
- 4 tbsp organic raw coconut oil
- Add in 20 to 30 drops of your favorite essential oil like Litsea Cubeba/May Chang
- Mix together, you may need to add more or less of the coconut oil to get the desired consistency. Store in airtight container, apply to underarms with fingers.
- More deodorant info here
- My deodorant blog category
- Options to add: Tea tree oil, other essential oils.
DIY toothpaste:
- 3 tbsp baking soda
- 3 tbsp coconut oil
- 3 to 10 drops essential oil (like 1 or more of these tea tree, thieves/germ fighter, orange, peppermint or eucalyptus)
- Mix & store in an airtight container
- Brush teeth as you normally would
- Add more or less of baking soda to create your favorite desired consistency, or just use the coconut oil to brush with or just coconut oil & essential oils of choice, make it your own.
- More toothpaste info here
- My DIY Oral care blog category
Body Butter:
- 1 or so cups of solid coconut oil
- 1 tbsp of natural liquid oil of choice (Jojoba, castor, fractionated coconut, argan)
- 5 to 20 drops of essential oil of choice like Lavender or Rosemary.
- Mix as directed here
- Apply to skin as you would creams & lotions, apply small amounts & rub into skin after bath or shower if going out in the sun or anytime needed. Very healing & has natural SPF. Hydrating to skin without toxins.
Facial scrub:
- You can simply use just epsom salt on wet face & work in & let sit till dry then wash off & rub in raw coconut oil,
- I have also used the Epsom salt mixed with the coconut oil together on skin 3 parts epsom salt to 1 part coconut oil.
- You can also try: Epsom salt with ground pink salt, Epsom salt with borax, epsom salt & baking soda, Epsom salt & honey, Epsom salt is awesome.
Face wash:
- 1/4 tsp coconut oil
- 1/2 tsp castile soap
- 1/2 to 3/4 tsp activated charcoal powder
- 1 to 5 drops of essential oils of choice
- Add 1/4 tsp raw honey
- Mix together in small bowl, apply to slightly wet skin/face & rub like you would with soap, rinse off or let sit a few minutes & rinse off.
- I mix right in my hand by adding to one palm then rubbing together.
- I also like to wash skin, then apply warm/hot damp cloth, lay over skin, let sit about 30 seconds then use the wet washcloth to clean the wash off.
Vapor rub/Chest rub:
- 1 tbsp coconut oil
- 1 to 2 drops eucalyptus essential oil
- 1 to 2 drops Pine essential oil
- Or use 1 to 2 drops Thieves/Germ Fighter instead of the eucalyptus & pine or use all 3 at 1 drop each
- Mix together in hand, apply to chest, throat, back, sinuses.
Affiliate & Referral links: I do when I can have links that are affiliate or referral links, not all links on my blog, pages & posts are affiliate or referral links. (More info here)
I will share links to buy products on Amazon anytime I can, I thank you for your support.
- Check out my Disclaimers & Policies page here
- If this post contains an alternative remedy remember I am not a Dr.
Follow me: Facebook – Pinterest – Google+ – Instagram (Raising6kids) – Twitter @NaturalCheree
Click here to learn where buy some of my favorite products such as coconut oil, colloidal silver & more.
All information contained in the pages of Natural & Frugal: Raising 6 kids blog – NFR6K Blog, blog posts, site, Facebook page, Twitter, Pinterest or elsewhere are for information & research only.
Natural & Frugal: Raising 6 kids & NFR6K Reviews
To contact me to review your product go here.
More products I love by Tropical Traditions: Gold Label Virgin Coconut oil – Green label Virgin coconut oil – Palm Oil – Palm shortening – Extra Virgin Olive oil – Black Cumin Seed oil – Organic Sesame oil – Coconut water Vinegar – Organic Apple cider vinegar – Balsamic vinegar – Pink Himalayan salt – Organic spices – Organic Whole Unbleached Sugar – Grass-Fed butter & more.
"If you order by clicking on any of my links & order from Tropical Traditions & have never ordered from them in the past, you might receive a free book on Virgin Coconut Oil, & I will receive a discount coupon for referring you"
I only recommend products or services I use personally and believe will be good for my readers. Thank you for reading my blog & supporting me on the journey to share #NFR6K Cheree


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The world of YouTube!5:12 PM
I took the plunge into the world of YouTube! Feels so strange but we'll see how it goes! Check out my first video here:
26-year-old Public Relations graduate living in Halifax, Nova Scotia. Sharing my love of beauty and occasionally throwing in my love for music, travel, DIY - and other aspects of surviving the beautiful east coast of Canada.
Oh, mascara. The controversial darling of the makeup world. There are some lucky folks who find their ride-or-die. The one they will st...


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(This piece was originally published on October 1, 2017 in the Flyer Group.)
For the first time, Greening the Statehouse will run from 9 a.m. to 4 p.m. Dec. 2 at the Hendricks County Fairgrounds. This is the organization's 10th annual meeting, and admission ranges from $10 to $25.
"This is the largest gathering of environmentally minded Hoosiers each year," Hoosier Environmental Council Director Jesse Kharbanda said of Greening the Statehouse. "It draws people from all across the state from different political backgrounds who are interested in finding solutions for protecting the environment."
Kharbanda said this year's event is special.
"Not just because it's the 10th, but because the public is now wondering if we're backtracking on maintaining environmental issues for a sustainable economy," he said. "As a means of addressing that concern we are bringing in one of the most prominent conservatives working to protect the environment."
That person is Mitch Hescox, who will be the guest speaker. Hescox spent his early years in the fossil fuel industry, but has since become one of the nation's leading conservative promoters for environmental activism. He is the head of the Evangelical Environmental Network and speaks regularly on renewable energy, clear air and clean water policies.
"The way that we're thinking about it is that in order to make real significant change to protect the environment, we have to make sure we're engaging people across the political spectrum," Kharbanda said. "At its peak in the '70s, there was huge bipartisan support in congress, and that's why the Clean Air Act was passed unanimously. It has now become a divisive issue, and it shouldn't be. Whatever your politics are, there's a basis for wanting to protect the environment."
Greening the Statehouse intends to provide attendees with a sense of Indiana's environmental landscape over the previous year.
"But we'll also be sharing what it is we see for the future," Kharbanda said. "This is different from prior years in that we're putting more of an emphasis on the tools that people can acquire to be able to make a difference in their communities."
Attendees will learn more about engaging in meaningful relationships with their elected officials that ultimately leads to progress for the environment.
Kharbanda describes the Hoosier Environmental Council as a big tent organization that seeks to engage people from every part of the state. As a result, both large statewide and smaller local organizations will be represented on Dec. 2.
Lectures will be presented and informational booths will also be stationed throughout the event area. Groups with a localized emphasis will be present and groups focusing on a variety of different issues such as renewable energy, environmentalism and agriculture, forestry and environmental justice will be available to answer questions.
"But the real centerpiece of the day will be all about people getting trained on how they can be more effective advocates for their community," Kharbanda said. "People will come away feeling inspired and empowered with new tools to make them more successful in bringing positive change to their communities."
Kharbanda said there are many elected officials who are taking up the mantle of responsibility locally. Mayors in North Vernon and Bloomington have championed ordinances that have utilized solar energy for much or all of their respective towns; Fort Wayne now has safer waste water treatment and Indianapolis has electrified much of the city's automotive fleet.
"Mayors taking the initiative like this are a great source of hope," he added.
For more information or to register for the event, visit the website at hecweb.org/gts2017.


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Cards and Payments Conferences
> Upcomings Conferences
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> Previous Conferences, Webinars and Training All Past Conferences >
This event is focused especially on digital banking, remaining the existing customers and attracting potential ones with the digital experience. PSD2 as a new regulation will bring a lot of challenges to banking industry. You will have an opportunity to see what the most innovative banks deal with.
By : Allan Lloyds group


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Phone number: 029981....
URL: http://www.allaboutyou.net.au
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Nearby Businesses within 5 km
- A 1.9 km Minds Eye Films
- B 2.3 km Pemberton Productions
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- D 3.4 km Shotgun Weddings Productions
- E 3.5 km The Quarantine Hauntings
Nearby Suburbs
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We are pleased to invite you to learn more about our unique immersion program and school community.
Our after school language classes provide a fun and effective way for preschool and elementary children to learn Mandarin or Spanish language skills.
To register online click your campus below:
Home > Students Learn About the Engineering Design Process > Engineering 3
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The VanDoodleZine
Shop Vandoodlezine
Be as Redneck as ya wanna be wearing The Vandoodlezine's Offical Gear!
Just click on the product to look at it in more detail , to buy or for more info!
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Help a Vandoodle out! By clicking on the Google Ads Vandoodle recieves a penny or two! No purchase
Please help The Vandoodlezine by visiting these sponsors! All proceeds go to CCC Academy, respectfully.


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Telling your story• Articles, stories, websites, and other resources about how people have told their own, their family, or their company stories)
• The art and craft of interviewing
• Great interview questions and guides
• Audio recording and editing equipment, software, and tutorials
• Capturing family voices
Multimedia -- scanning and adding images, sound, music• Video and multimedia tributes and documentaries (good examples online)
• Video production and editing
• Sources for music, images, video clips and related material
• Finding background music for video biographies, podcasts, presentations, blogs, etc.
• Finding vintage music from a particular year or place
• Finding photos and other images
• Scanning photos, documents, and other images
• Scanning, repairing, and organizing photos
• Audio recording and editing equipment, software, and tutorials
• Audio editing software and instructions
• Audio tools, technology, and tutorials
• Mastering multimedia
Archiving, conservation, and preservation"Never dump originals - they may be all that is left after the computer age."
~Peter Amsden, via Taylor Whitney • Preserving your family and national treasures
• Archiving resources
• Books about preservation
• Adding metadata to photos
• Suppliers of archival materials
Oral histories and video interviews• Doing oral histories or video interviews
• Oral history collections online
• Online guides to doing oral history
• Transcribing oral history interviews
• Books about doing oral history
• Oral history as social history
• Stories about oral history
• Further reading about oral history
Booklists• Books to help you get started writing your own (or someone else's) life story
• Writing personal and family histories (a booklist)
• Memoir writing as discovery
• Memoirs, healing, and self-understanding
• Writing from memory prompts
• Anthologies of life story writing and reminiscence
• The art and craft of memoir and biography
• Books for life story writing and reminiscence groups'
Memoir, biography, and corporate history• Memoir, biography, and corporate history
(Writers and Editors site)
• What is the difference between a memoir and an autobiography (or memoirs)?
• Organizations and gathering places for
biographers, memoirists, personal historians, and other life story people
• The craft of life story writing
• The nature and malleability of memory
• The art, craft, and politics of biography
• Memoirs, memoir writing, and autobiography
• Writer's Digest series on memoir writing
• The life story business and market
• Become a personal historian--help others tell their life stories
• More about personal histories and legacy memoirs (primarily for the family)
• Writing corporate and organizational histories (commissioned histories)
• The self we tell ourselves we are influences our decisions
Memoirs (recommended reading)• Coming of age memoirs
• Memoirs and personal accounts of
vocation, avocation, occupation, profession, calling
• Memoirs of regular people
(and lives lived outside the limelight)
• Memoirs of friendship, family, and other relationships
• Graphic memoirs
• Food memoirs and biographies
• Memoirs of illness, crisis, disability, differentness, and survival
• Memoirs of (and other books about) caregiving
• Memoir- and life-story-related sites
Ethical wills (or legacy letters)a/k/a life letters, heart wills, ending notes, love wills, testaments)
• What is an ethical will?
• Examples of ethical wills
• Why people create ethical wills
• How-to books
• Dignity therapy
• Ethical Wills (Pat McNees, blog entry on APH website)
• Kristie Miller's Letter of Intent
Pat McNees's three websites• Dying, Surviving, and Aging with Grace (not necessarily in that order)
• Writers and Editors
• Pat McNees (personal site)


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Home | Learn Acting | Film Actors Biographies | Contact
How to Become A Model | Sayings |How to Face Audition | How to get Casting calls information | Portfolio and Directors Mentality |Coming to city of Dreams | 11 Acting and Modeling Audition Tips | An Actors life and Goals |Everything you ever wanted to know about Method Acting | Get your kids into Acting Business | Want to be Hero or Villain ? | Useful Acting Tips | Join Acting Workshop
Method Acting
Learn Everything you ever wanted to know about Method Acting
Method acting is an acting technique in which actors try to replicate in real
life the emotional conditions under which the character operates, an effort to
create a life like, realistic performance. "The Method" typically refers to the
generic practice of actors drawing on their own emotions, memories, and
experiences to influence their portrayals of characters. was an American
director, actor, producer, and acting teacher
Konstantin Stanislavski, (1863-1938) Who pioneered idea and called it the "Method of Physical Action" it was purely based on "recollection of emotion". Actor was suppose to act in given circumstances, the exercise of the imagination, and the use of physical action. But few thing were modified from this method by Lee Strasberg mainly evolvement to rely on Physical Action inducing feelings and emotions
"Method Acting" name was given by Lee Strasberg between 1940 and 1950.
Lee Strasberg was an American director, actor, producer, and acting teacher
Strasberg's students included many of America's most famous actors of the 20th century, including Paul Newman, Al Pacino, James Dean, Dustin Hoffman, Marilyn Monroe and many others.
Lee Strasberg suggestion to the Students and theater film performers was "try not to act, be yourself, use gesture in a manner that you use in private life".
Some consider Method acting difficult to teach. because of a common misconception that there is a single "method." Stanislavski himself changed his method constantly and dramatically over the course of his career. This made it hard to teach a single method. In general, however, method acting combines a careful consideration of the psychological motives of the character and some sort of personal identification with and possibly the reproduction of the character's emotional state in a realistic way.
Those trained by Strasberg often tried to experience all sensations as the character would and often used personal experience on stage and films to identify with the emotional life of the character and portray it.
Some useful links where you can know more about Method Acting.
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Coming up on First at 4:00:
Federal regulators have approved new supplies for a pair of crucial cancer drugs. Rachel DePompa has details on this health alert.
Deputies are investigating a string of arsons in Caroline County. Ben Garbarek speaks with one man whose house was vandalized.
Plus, Matt Lincoln has the latest from the America's Next Top Model casting call at Virginia Center Commons.
Then at 5:00, Sabrina Squire visits New Hope Rescue in our Acts of Kindness segment.
Those stories and more are coming up, starting with First at 4:00.


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Alicia Silverstone and her adorable son Bear Blu were spotted at the Los Angeles Farmer's Market on Sunday August 28th, the mother and son appeared to be having a blast together, and the happy toddler… [Continue Reading]
Alicia Silverstone And Bear Blu Soak Up The Sun In Sydney
Alicia Silverstone's handsome little boy Bear Blu Jarecki is growing up so fast! Bear just turned 5 years old on May 5, Alicia and Christopher Jarecki announced the birth of their son back in 2011…. [Continue Reading]


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Gerard Pique slid Marcelo's cross into his own net before Lionel Messi levelled from a penalty.
Ronaldo, who was a substitute, restored Real's lead when he ran from halfway before smashing an effort home.
He was booked for taking off his shirt to celebrate and shown a second yellow shortly after for a dive - before Marco Asensio scored an excellent third.
Ronaldo felt he should have had a penalty when he went down under pressure from Samuel Umtiti, and appeared to shove the referee in the back after he was shown the red card.
Cristiano Ronaldo scored and was then sent off as Real Madrid beat Barcelona 3-1 in the Spanish Super Cup first leg.
Real host Barca in Wednesday's second leg at 22:00 BST.
The second leg will be played three days later on 16th August 2017 at Santiago Bernabue.
Watch Match Highlights Below:


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Contact Us
If you have any issues feel free to contact us at firstname.lastname@example.com.
Need to return or exchange your Vibrant bra?
No problem.
Start A Return
- Vibrant will pay the shipping costs on all returns and exchanges.
- For many women, the Vibrant bra feels different than bras they currently wear. That's a good thing! Before you decide to return it, we encourage you to wear it more than once.
- To receive a full refund:
- The bra cannot be damaged, stained or discolored.
- The bra cannot be machine washed (hand washing with a non-toxic soap is recommended, but please do so after you've committed to keep it).
- Your bra is postmarked to us less than 30 days after your purchase date.
- To exchange for a different size, please indicate your preference on the return/exchange page/packaging insert and include it when you send your bra back. We'll take any appropriate packaging. We'll process your new bra as fast as we can, and will contact you via email when it ships.
- Refunds for the entire amount billed to your card will be credited to the card you used to make the original transaction.
- If you received a bra that you think may be defective, please contact customer service immediately! We'll make it right!


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3, 4 and 5 Bedroom Newly Constructed Homes for sale in Conway, South Carolina
Barons Bluff offers new single family homes for sale, combining the best of two worlds. Located between the quaint historic river town of Conway, South Carolina and the many attractions, beaches and shopping of exciting Myrtle Beach, less than a fifteen minute drive away. Come discover what many others already have. Low maintenance energy efficient homes at very affordable values. A natural gas community. Lake views and wooded views available.
Creekside Custom Homes
~ A Foundation of Honesty and Integrity ~
Creekside Homes, founded in 2004, has built an exceptional reputation. Whether it is a customized home or a commercial project, Jamie McLain strives to deliver the clients' goals and puts forward the best product possible.
Since founding Creekside Homes, Jamie has designed and built spec, semi-custom and highly customized homes in a wide size and price range in planned communities and on individual sites along the beaches and in inland areas of Horry County.
Most of Jamie's subcontractors have worked with him since 2004. "Their sweat is what makes me look good," he says. "I consider them family. We're just family that travels around building houses. We build together, and we do the best job we can for folks."
Like many generations of his family, Jamie was born and raised in Horry County. Although the financial crisis that began in 2008 caused many companies to close, Creekside Homes was able to continue to build. The high quality of work and the diversity of the company are among the primary reasons Creekside Homes has remained successful.
Jamie earned a degree in forestry from Horry Georgetown Technical College in 1997 and a bachelor's degree in forestry from Clemson University in 2000. He returned to his hometown of Conway and was involved in environmental work before founding Creekside Homes and continues his commitment to the environment. According to Jamie, "we're environmentally conscious and try to recycle everything we can. It's a shame for it to go into the landfill when it can go back into some other product".
Living a balanced life is part of what makes Jamie the calm and capable designer and builder his homeowners describe as they spread his reputation and become his best advertisers. Since family is important to Jamie, creating homes and communities that families can enjoy is also important to him. He enjoys spending time with Helen, his wife, and their son, Jase, and attending Trinity United Methodist Church.


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Courage does not always roar.....sometimes it is the quiet voice at the end of the day saying....."I will try again tomorrow".
Thursday, February 11, 2016
A Month of Letters - Day 11 - Lois Hannan
I have done that as well so I wonder how many I will get as surprises.
February is a Month of Letters where we send at least one piece of mail a day via snail mail to someone.
2 comments:
Lovely card of your wildlife Bridget - sorry I haven't been commenting quite as much - life just got really hectic the past few days.ReplyDelete
Another lovely card. I hadn't realized that you have put your name down to send cards to other people. I do hope that many others will pick your name and send you a surprise card.ReplyDelete
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Big & Tall Short Sleeve Linen Solid
Details https://www.belk.com/p/nautica-big-tall-short-sleeve-linen-solid/3201634M74145.html
Orig. $75.00$14.39 14.39
Additional Information
Take your cool and casual standards to the next level with this tropically detailed shirt. A lightweight linen profile makes this versatile piece the perfect pick for a walk along the dock or a seaside excursion with friends. It even boasts a classic fit, creating the kind of comfort that goes great with a warm weekend breeze.
- Button closure
- Short sleeves
- Classic fit
- Linen fabric
- Cotton
Shipping Details
Shipping Details
Shipping your purchase is easy at belk.com. Depending on your shipping method and the destination of your package, you can expect it to arrive within 4-7 business days Shipping Information.
Return Details
Returns are easy at belk.com. Easy returns
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I find myself sometimes still struggling with my obligation to buy less. For example handbags and jewellry. I have to ask myself the question: do I really need this? Then I consider the money – how much good could this amount of money do to a person less fortunate than me? And, wouldn't this handbag become just another bad buy and pile up in my closet or I have to sell it at the internet for a quarter of the price I paid? And what about the global problem of waste? The more I buy, the more waste I generate.
For some reason it appears as though buying new things is a way of being nice to myself. Whenever I'm feeling somewhat sad or down, spending money on items I really don't need, have been the main way in which I've tried to make me happy again. During the last months, however, it has become clear to me that what makes me happy is not buying new things, but being with my son and my dog. I guess I've known this for some thime, but haven't done anything about it before this lent. My buying stop during lent was really fruitful, and now it's easier to not buy than buy. Because when thinking about buying an item I really don't need, I get a bad conscience! And that helps me alot.
My want to contribute to a better world, a world with a more fair ressource distribution, obliges me to do and not do certain things. And though they may feel like a unneccessary sacrifice at the time, I know that it's the right thing to do. And in the long run it will give me a better conscience. Knowledge should oblige one to act!
The parish priest always emphasise the fact that believing must be followed by action, otherwise one cannot say one believe. That's a good thing to remember!
Filed under: Handbags, Lent, Random life, Religion, Society | Leave a comment »


text: Causation and catastrophic injury: when a late report really is too late 08 November 2013 Laura-Johnson (0) At 2am on 26 January 2006 Jonathan Boyle was at a bus stop on Grove Road in East London with a friend. They had been out that evening and had been drinking. At the same time a police car was being driven south along the road on the way to deal with a pub fight, although not being driven with lights or siren or in any sort of hurry. The driver was travelling at about 33 miles per hour. As the driver approached the bus stop the Claimant suddenly fell from the pavement into the path of the vehicle. The vehicle collided with him and he suffered life changing spinal injuries. The Claimant brought a claim against the police: Boyle v Commissioner of Police of the Metropolis [2013] EWHC 395 (QB). At trial in February 2013 Turner J rejected the Claimant's primary case that an incident was occurring at the bus stop, at or around the time of the accident, that should have put the driver on notice that someone might suddenly enter the road, causing him to react in a way that would have enabled him to avoid the accident. Turner J did find the driver to be in breach of duty for travelling at 33mph and concluded that he should have been travelling at around 5 miles per hour more slowly. The claim was dismissed however because the Claimant had no medical evidence to suggest that his injuries would have been less had the driver been travelling at an appropriate speed. The Claimant served a report on causation from a spinal surgeon on the Friday before the trial was listed to start on the following Monday. He made an application on the morning of trial to rely on that evidence and, if necessary, to adjourn the question of causation to be dealt with at the quantum hearing, with the trial to address only the issue of breach of duty. Turner J refused the application on the grounds that it had been made too late and that the report did not assist him in determining the issue in any event. The Defendant's solicitors had repeatedly drawn the attention of the Claimant's solicitors to the fact that they had adduced no evidence on this issue and no explanation was given to Turner J about how the report had come to be served so late. The Claimant appealed and the Court of Appeal dismissed the appeal on Tuesday. The decision is of some wider interest because the Court placed weight on the wider prejudice caused by such late applications not only to the Defendant but to the system of justice as a whole and to waiting litigants. Although Turner J's decision was made before 1 April 2013, the new climate in litigation of adherence to rules was taken into account. Longmore and Black LLJ concluded that although the judge had a discretion to admit the report even though it was served late, he had considered the relevant rules in CPR Pt 35 and the factors enumerated in rule 3.9 so far as relevant. It was impossible to say that the judge had gone wrong in his analysis. No explanation had been given for the delay and the Defendant had invited the Claimant's solicitors to serve the relevant evidence. The judge had not erred in conflating the issue of whether the report should be allowed in and whether it was adequate for its intended purpose. The judge was correct that there was no point in serving the report if it did not assist. The report appeared to do no more than state the obvious, namely that if the speed at impact had been less the Claimant would probably have been less seriously injured. The expert's evidence might have been useful if he had given oral evidence or there had been a meeting of experts, but neither course would have been consistent with the permitted way of deploying expert evidence which required the sequential disclosure of reports. The Court of Appeal concluded that adjourning until after the court had made a finding as to the speed at which the driver had been travelling might have been sensible at some earlier stage but was contrary to an earlier master's order providing for a split trial and would have left the court to decide the issues of breach of duty and safe speed in a vacuum. It was not right to contemplate such an outcome so late in the day. There had been a prolonged and persistent failure to serve the evidence. The judge's decision was properly arrived at and he was entitled to find that the Claimant's case had to fail. Edward Bishop QC and Laura Johnson successfully represented the Commissioner of Police of the Metropolis both at first instance and on appeal


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mAssigner- Group To do & Task List for iPhoneFree
Free Monica Manchanda iOS Version 1.2 Full Specs
Publisher's Description
From Monica Manchanda:
mAssigner is a next generation to do list and group task management solution (FREE for limited period) that helps you manage your own task list / To do list or assign and delegate To do to your colleagues, friends or family members using their mobile contact information from your mobile device. First of it's kind To do/tasks list management solution!mAssigner features an intuitive design and a simple reminder and alert system to ensure that you will never miss a deadline again! Keep track of important project deadlines, build your grocery or shopping list for your household, and plan events with your friends.Key Benefits & Features:* "Work together, not in isolation" with easy-to-use collaborative tools* Intuitive design that incorporates familiar features from email* Assign To do to contacts already in your mobile device ( a patent pending feature)* Comment on tasks to communicate with your team member* Convey urgency or completion with a single click* Manage your deadlines with helpful alerts and reminders* Cloud sync across multiple platforms so you can access your To do list anywhere you go* Handles to-do assigned from unknown mobile numbers* Latest or recently updated To do remain on top for easy accessibility* Notifications for all actions and comments for your to-do* Tracks and logs To do and comments* Tracks assigned To do of each individualNew features coming soon! Do you have suggestions for a feature you would like to see in the mAssigner To Do List & Task Manager? Let us know, we are eager to hear your feedback!For news and updates you can follow us on Twitter and Facebook:http://twitter.com/massignerhttp://www.facebook.com/massignerWatch our videos at:http://youtube.com/user/massignerNOTE: Please do visit our terms and condition page- http://massigner.com/TermsPrivacy.htm
read more +


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Construction activity often imposes or relieves loads on adjacent structures potentially causing movement and damage to the structure. In the worst cases, catastrophic failure can lead to a loss of life. For this reason monitoring of the adjacent structures is crucial to the protection of the structure as well as the contractor performing the work. CEC primary focus is providing monitoring in compliance with NYC Building Code 3309.16 and the necessary documentation required during inspections.
Full service Monitoring
CEC provides cost-effective full service monitoring of adjacent structures including:
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HoHoe (Ho Hoye) – Gateway to Nature
There wasn't much on the net for accommodations, so we were just going to turn up in the Volta region for nature, and find a spot. We found a decent spot, Pacific Guest House for GHC$40, so 10 bucks!
We had been craving to leave the cities, Accra is so massive and Cape Coast is a small enough city you don't feel bombarded, but we needed some beauty and hear the Volta region with it's mountains and huge lake was where it's at!
Having been stung by a wasp and twisted my ankle on a hike in Kokrobite, I needed some chill recreational activities, so what do we do? Climb a mountain, and like American Ninja Warrior, climb a mountain!
Coming into Hohoe (which the locals call 2H city), I was immediately drawn to the surrounding mountains, all lush green and engulfing. We trekked out to Wli Waterfalls, and climbed to the upper falls. For this half-in/out of shape fatty, it was a chore, and was thinking the reverse climb as going to be pretty wicked as well. I was right, the other hiker with us fell a couple time, and I slid on my rear a couple times. Our guide said no one has (yet) to be carried out, so I had hope, with a sore rear. Being that I have no rumpshaker….
Approaching the upper falls, I thought it was raining in the jungle, the power of the falls was so strong, it was a whole other climate/ecosystem up there. Getting close to the stream I could peak up and see the origins of the falls, and it seemed to honestly come from heaven. Wli is the tallest waterfall in Western Africa and I climbed that biatch!!!!
The devilish forces (as we call them in my buddhist practice) were correct, and the reverse climb was a beast, all of our legs felt like jelly, except our "guide" who was fortified by drinking from the waterfall. We pissed our "guide" off so much because we wanted to take our time, and not just hoof it up the mountain. Stop, look, take in the splendor, take a pic, eat a banana, hydrate. I say "guide" because he offered no education to the experience, and when asked, didn't really know if this WAS the tallest waterfall in Western Africa, where the water source came from….so he was there to run ahead to the tourist office to let them know if we died or not.
Tafi Atome..In the monkeys we believe
There's a monkey sanctuary in Tafi Atome. It's showcased as a place of ritual. Here the local village worship the Mona Monkey as their religion. They believe that the Mona monkey is a messenger to God, communicating their prayers to God.
There's not many that still 100% praise the almighty Mona, but praise Jesus the almighty instead, and have respect for the monkeys. There's a shrine and a king of the village that accepts gin for libations.
With the proselytization of Christianity in Ghana, a lot of monkeys were killed in the beginning, as it was seen as a pagan religion, but now there seems to be a symbiosis of traditional religion and Christianity. There is SO much God in Ghana! We've been woken up by midnight services, and there's a church service, rally on every corner every day of the week.
I love the fact that tradition can still exist and it's not driven out with the possible bad word- Pagan, it's tradition, it's deity, it's what they believe.
Tafe Abuife..The Kente Weaving villages
After visiting the monkeys, we headed to the Kente weaving village called Tafe Abuife. The only available transportation was a single motorbike for the three of us, including our driver. Three people on a bike, hmmm. I have seen four on a bike, while people carrying stuff so this didn't come as a surprise at all. It took about 20 min to get there.
We were greeted by Francis, our guide. Started the tour by a history of Kente weaving. Kente is pronounced as (Key Tay) by the locals, but since most foreigners failed to say 'Kete' correctly and end up calling it Kente, it became the actual name.
In the Ewe village we visited, we were told that Kente weaving started by a hunter who saw a spider spin its web and told his people and the king what he saw, and led them to the spider. After studying how the spider spun it' web they decided to try it themselves. They erected poles and used cotton by making a thread out of it and weaving it across the poles. And according to the Ewe, that's how Kente weaving started…the Ashanti have a different version. http://www.ghanaweb.com/GhanaHomePage/features/The-Issue-of-the-Origin-and-Meaning-of-Kente-aka-Kete-367807 for more read.
Wearing Kente used to be reserved for the Royal family, but today everybody can own Kente cloth. It's worn for holidays and special occasions, though.
Out of the 4 villages that weave in the Volta region, every household in the village owns a loom and children start learning how to weave at the age of 4 or 5. Most people make a living out of it. It takes 3 years of apprenticeship before the village will let a Kente weaving artist sell.
We also saw a factory with about 20 weavers, weaving different kinds of Kente. We were shocked to see only one woman weaver. Not all men are meant for the farm….There is three kinds of Kente design. They call them the single, double and triple woven. The triple being the most complicated and expensive.
The single has a matte design throughout, the double switch between matt and other designs and the triple has all designs and no matt. All the designs tells a certain story, typically dealing with carpentry, farming, family history. The weavers, as they progress in their trade are allowed some creative freedom, and make their own patterns and colors, in line with the traditional story of their liking.
For example on the piece that we bought below the zigzag blue lines are called the 'footsteps of our forefathers.' It tells the story of a wicked king that oppressed the people and they want to leave the kingdom. But they weren't allowed to do so by the king. They decided to leave anyways, and started walking backwards in a zigzag fashion to avoid getting attacked from behind. This pattern is called 'sheep's eye' and represents a slaughtered sheep that used to be sacrificed by the people to the gods.
More at https://www.kentecloth.net/


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text: Grant, We have a GLX (AWD, V6) as a company car for our VP (purchased new). Great value as it's a total Audi drivetrain. The FWD versions seem to have more differences (suspension, etc.). Our 2001 is about to get replaced. It's been stellar, but it's around 200K miles now! My mother's BMW 330xi will likely become his new car as she wants something bigger. The climate control head should be scannable with VAG-COM. If there's a stepper motor failure, it'll surely turn up there. And that's where you should start the diagnosis. When he changes the cabin air filter, it would be a good opportunity to check for debris (leaves, dead rats) as well. Mark Rosenkrantz ('87 4KCSQ, '00 S4, and a bunch of other toys) On Dec 24, 2007 9:10 AM, Grant Lenahan <glenahan at vfemail.net> wrote: > OK, its a Passat. and it isn't even mine. > > But a friend, who's a pretty fair mechanic, just bought a 2001 Passat > for his son. It should be very similar to B5 A4s and S4s in this > regard, I assume. > > One problem is that the heat doesn't work - it blows stone cold air > (I'm told). They know the history of the car, and the owner says it > was working normally one day, and totally gone the next. We suspect a > mixture (cold/warm air) stepper motor died or a connection to it has > a problem. But i've not heard many complaints about this. > > Any wisdom? Common problems? Things to check? Warnings? > > Thanks much, > > Grant > > ps: apologies if this arrived twice. It got caught in moderator > approval land and never emerged - or not so I saw. > _______________________________________________ > Biturbos4 mailing list > Biturbos4 at www.audifans.com > http://www.audifans.com/mailman/listinfo/biturbos4 >


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In places like San Jose, "new approaches to the storefront" are paying dividends for physical retailers willing to experiment.
City Observatory illustrates a key contributor to a vibrant urban environment by mapping all of the businesses in the urban cores of the nation's 51 largest cities.
City Observatory
The quality of L.A.'s public structures falls far below its remarkable private residences. Greg Goldin argues why we should see the beauty in its greatest creations: its infrastructure and evolving collection of noisy storefronts.
Zocalo Public Square
How to Be A Retronaut features a smattering of photos of New York's unique storefronts, taken by photographers James and Karla Murray. Since the book's release, about a third of the stores have faded from existence.
How to Be a Retronaut
With small businesses closing shop and empty stores lining its main street, the British borough of North Tyneside is creating fake storefronts to create the appearance of a lively shopping area.

Mixed-use projects are all the rage, but often times, the ground floor retail storefronts remain empty long after residents have moved in.

Dallas Morning News


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text: Subscribe to our newsletter This thingy has to be empti pwieshh Deep Learning Weekly - 🤖 - Issue #57: Unsupervised Learning, Brain AMA, Video Object Segmentation, Tensorboard Plugins, API security and more... Revue Hi and welcome to a new week in deep learning, In this issue, we bring you Google Brains latest AMA, September 15 · Issue #57 · View online Hi and welcome to a new week in deep learning,In this issue, we bring you Google Brains latest AMA, take a look at the current state of unsupervised learning, get you started on video object segmentation and try to detect malicious API requests. Furthermore, an extensive survey on music generation using deep learning was released, Fast.ai has announced the use of Pytorch in upcoming courses and Tensorflow now offers a plugin based system for their Tensorboard visualizations. Happy hacking and reading. As always if you enjoy receiving this newsletter you can help us by sharing it with friends and colleagues.See you next week! Industry In search of the missing signals giorgiopatrini.org – Share In this well-written overview, Giorgio Patrini explores the current state of unsupervised learning and shares his thoughts on some recent approaches and techniques. The way he explains the papers allows you to quickly jump into the field and get a feel for the different trends that came up recently. Definitely worth a look! We are the Google Brain team. We'd love to answer your questions (again) www.reddit.com – Share The Google Brain team has decided to do another AMA on Reddit, after last years one was very successful. They share their opinions on Pytorch, try to anticipate upcoming challenges and hurdles, e.g. unsupervised learning and solving multiple task with a single model, as well as insights into the daily life at Google Brain. AI can liberate humans to lead happier lives, if we get it right www.cityam.com – Share Another take on the heated discussions around the impact of AI on general life and the workplace of the future. This time a little more balanced and not the usual apocalyptic approach. A four-day working week seems like a great argument for AI. Learning Video Object Segmentation — The Basics – Eddie Smolyansky – Medium medium.com – Share This is the first of two articles on video object segmentation. It covers the basic concepts, existing datasets and last years main approaches, as well as a new dataset made by Visualead and focused on e-commerce. A great read if you want to get into video object segmentation or quickly need to get up to speed. Don't forget the second article for more advanced insights. Detecting Malicious Requests with Keras & Tensorflow medium.com – Share This article describes the results of a recent hackathon, where the team built a machine learning system that tries to classify API requests as regular and malicious. Interestingly they opted for a NLP based approach and the resulting model seems quite efficient, but the lessons learned are interesting as well. Libraries & Code Introducing Pytorch for fast.ai www.fast.ai – Share Fast.ai will begin using Pytorch instead of Keras and TensorFlow for their upcoming courses and explains the motivations behind the change. The dynamic aspect of the framework seems to speed up adoption quite heavily, so why not take a look? GitHub - fyu/drn: Dilated Residual Networks github.com – Share Speaking of Pytorch, this repository contains a collection of residual neural networks. They're able to achieve good results on different datasets and may be useful for your next project. Build your own Machine Learning Visualizations with the new TensorBoard API research.googleblog.com – Share Google has released a new API for TensorBoard that allows creating new visualizations using a plugin based system. An initial set of new plugins using the API is already available and may be used as a starting point for your own implementations. Papers & Publications Deep Learning Techniques for Music Generation - A Survey arxiv.org – Share Whenever a specific field grows exponentially, it can become really hard to grasp all available approaches and put them in perspective. Thanks to this recent paper one can now get an overview of all recent works on the generation of music using deep learning. NIPS 2017 - Accepted Papers nips.cc – Share This years accepted papers have been announced and you can now get a sneak peek at what's to come. Definitely a lot of interesting titles in there. Did you enjoy this issue? If you don't want these updates anymore, please unsubscribe here If you were forwarded this newsletter and you like it, you can subscribe here Powered by Revue Share Share to Twitter Share to Facebook Subscribe to our newsletter This thingy has to be empti pwieshh


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On Sunday night I had a wonderful encounter that came about by the most mundane of circumstances: a ride on the London Underground.
During my internship in London this summer Tom and I went out for dinner in town one evening after work. After having a bit too much to drink we ambled down to the tube and arrived just in time to dash into a carriage as the doors were closing rather than waiting a whole two minutes for the next one -I'm told pretending to be in a hurry all the time is the done thing on London transport.
In the carriage Tom noticed a couple, one of whom was wearing a Warwick University hoodie so he decided to strike up a conversation. In the time it takes to travel two tube stops on the Northern line we chatted about Uni and life after Uni and briefly exchanged emails before parting ways – they were from Hong Kong and by that point I knew I was moving to Shenzhen.
About 2 months later, I received a message from them saying they were around and asked if I wanted to meet up for dinner. I thought OK cool, some dumplings, meat and veg in a local somewhere would be great. We loosely agreed a day and that was about it. On the day I received a call from them and we arranged a time and metro stop at which to meet. I went along and found them with a car and driver. As we drove all the way to the other side of the city I realised they had come to meet me there because that's where I was, not because it was in any way convenient.
We pulled up to this lush place where the driver opened the door for me and helped onto the red carpet that led up to its entrance. It was the one place in Shenzhen I had actually heard of. It's the one place all foreigners have heard of as it is one of two Shenzhen attractions. It wasn't the designer bags so it had to be the spa. It was all lit up and inside there was glass so clean that it sparkled. We went into the lift and the doors opened to the restaurant. There were tanks with exotic sea creatures swimming around by the entrance and we were led to our table which was in a tastefully decorated private room at the back of the restaurant complete with own private restroom. We were greeted by the head Chef who discussed our food options with the couple (or at least that's what I'm guessing they discussed since the conversation was in Cantonese). The director came in to chat with us for a while too as he was his father.
Then the food arrived and the spread was fantastic. There was sashimi, wasabi strong enough to blow your brains out, stir fried frog, noodles, veg, roast goose (which is a rarity) with a tasty sharp dipping sauce, sweet buns, tea, mango smoothies and Kung Fu Soup in cute individual teapots – it's so named because of the slow and assiduous labour required to make this dish. This was a spread like no other. The kind you would reserve for a reunion with an old friend.
Despite having known each other for less than 10 minutes before hand, this couple treated me like an old friend. They explained to me that this is how it's done in the East. Coming from a place where if you met a person on the tube again it would be by chance and you would exchange a knowing nod or an awkward 'hello' at most, I have to say I was really touched by this show of kindness. I think we in the West could learn a lot from the East.


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Petzl Core Rechargeable Battery
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This rechargeable battery works well in low temperatures and is compatible with TIKKINA, TIKKA, ZIPKA, ACTIK, ACTIK CORE, TACTIKKA, TACTIKKA + and TACTIKKA +RGB headlamps.
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RELATED: Chris Hogan is back at practice! Click here for the latest update on the wide receiver returning from injury!
Colin Cowherd is at it again.
The often outspoken FS1 radio host talked about the New England Patriots on Wednesday, making an extremely bold claim that a large part of the Patriots dynasty comes from pure luck.
Cowherd, on his show, outlined the era beginning with the hiring of Bill Belichick in 2000, extending through the Patriots' Super Bowl victory a year ago and into the present season where the Pats are again one of the league's best teams.
He specifically outlined four things that somehow aligned perfectly for the Patriots that have enabled the team to go on such a dynastic run. It's one that he says will never happen ever again in the NFL.
The first lucky break was the hiring of Bill Belichick:
"There are four things that will never, ever be aligned like this again," Cowherd said. "They have the ultimate, brilliant chip-on-the-shoulder head coach. Bill Belichick, living in the shadow of Bill Parcells, has had a chip on his shoulder forever.
"And you know what the chip on the shoulder does for people? It is a lifetime of drive. So the next great head coach happens to be in New England with a massive chip on his shoulder. Doesn't want to write books, doesn't want to be on TV, doesn't want to do press conferences, he's all football."
The next break was a chip-on-shoulder quarterback:
Next page for more strikes of luck for the Patriots and to see the video >>>
4 reasons we'll never see anything like the Patriots dynasty again pic.twitter.com/8t32MzXcut
— Herd w/Colin Cowherd (@TheHerd) December 6, 2017


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Busy buying holiday weekend
By theoldhomestore, Oct 13 2015 12:00PM
Q. When is a holiday not a holiday?
A. When it's Columbus Day!
Lots of special events this weekend. Some kids off school, some not. Some people at work, some not. Anyway, lots of things about to do and lots of junk to buy - so that's a good thing.
Dade City Antiques Faire (with an e) proved very fruitful for primitives, but all geared up for Halloween at the moment. Was hoping for some prim dolls, but mostly witches and cats - not the scary santas and angels just yet. Great kitchenalia.
Websters Flea Market was much busier due to the holiday, and slightly more comfortable weather. Again, lots of nice things to bring home for TOHS.
These make the last big buying trips for this visit. It has been great. I hope that I can get it all home! If not, it will have to stay in the cupboard until January, when TOHS will be out again - as TOHS devotees are going to buy it all for Christmas - aren't you!
Web feed


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|Free bin. Small. Plastic. Liked it.
Oh well
|Sleeping Bag. Very good condition.
Has domes at the bottom.
|Cute handwriting on the label.
I'm not looking forward to the day when everyone who knows how to write like this has passed on.. I don't think there's a huge focus on handwriting these days. (computers can now do the job)
|Assisi frock.
They were trying to sell this for $30.
I got it in their closing down sale.
I believe they were a casualty of the Op Shop-Tries-to-be-a Vintage-Store thing. I liked them though. And they did have some bargains sometimes.
|Another Assisi frock. They are twins.
same label, same size, belts missing.
|skirt $1 at Assisi.
|Having just found Valerie Monk's blog
(She is Crown Lynn royalty if you didn't already know- author of the Crown Lynn book, and apparently another book on the way, hurrah!! And she commented on my blog! So stoked! Apparently my Prince Wills mug is rare. Thanks Val!)
I had spied this mug on her blog and thought how I'd never seen that pukeko design before..
Then I found this at the oppie the very next day! $2
|kitsch but I love them. Oil and Vinegar.
|Trivet - not my usual aesthetic, but I was quite drawn to it.
. But it is actually very handy and I've been hunting one.
So that's it!
Wonder what this week will bring?!
Collection linky post coming up this week after a miss last week!
Happy Days!
Linking up to
* Apron Thrift Girl
* A Living Space
* Blackbird has Spoken


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They're perfect for sponge cakes and also ideal for fruit cakes up to about 12". For larger or heavier cakes we recommend you use the tougher drum boards.
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