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Recent Articles and Notes about Quincy High School
Date: April 22, 2009About: Lee Lindsay Curtis - Class of 1960
Never Seeing the Obstacles
Source: Quincy Herald-WhigAuthor: Holly Wagner
Lee Lindsay Curtis accepted the 12th annual Joe Bonansinga Award for Community Service with her characteristic grace Tuesday by turning the accolades back onto the Rotary Club and the community's many other volunteers for their contributions to Quincy. The Rotary Club and United Way present the award as just one of the many ways they honor the man who became known as "Mr. Quincy." The United Way and past award recipients choose someone they believe best exemplifies the qualities of volunteer service that Bonansinga was known for."There are clear similarities" between Curtis and Bonansinga, said Harry Marcionetti, senior vice president of operations at Knapheide Manufacturing and president of the United Way board of directors. "Both exude passion for their community ... never seeing the obstacles."Marcionetti, who presented the award, praised Curtis for her teaching career in special education and her continuing work with Chaddock, Habitat for Humanity and Court Appointed Special Advocates (CASA). A mother of two and grandmother of four, she and her husband, Michael Curtis, own Great River Aviation. Lee Curtis is a licensed pilot and teaches at Quincy University. She holds a master's degree and continues to study at Western Illinois University.After retiring from teaching, she focused her energies on the community, Marcionetti said. At Chaddock, she is involved with the students as well as serving on the board and foundation. She works alongside the students on houses for Habitat for Humanity, an organization she has served for 14 years as president.In that time, Habitat has built more than a dozen homes for needy families in Quincy. For the past 10 years, Curtis has also volunteered with CASA, advocating for at-risk children involved in the courts.Curtis also has supported the United Way's Unmet Needs Partnership with generous gifts for many years. She has served on numerous boards and committees and received honors for her contributions."Lee Lindsay Curtis sets the standard for excellence in our community," Marcionetti said. "Her impact on our community has been significant ... Her motivation to inspire others is a gift."You have given us the finest example of how to live and how to give back," he said.Curtis thanked United Way and Rotary for the honor and spoke of the people who had inspired her to follow an example of giving -- an example that began with her parents and has continued through the generations of her family. That spirit also lives in the people Curtis calls friends and chooses to surround herself with. It exists in the people who participate in Rotary and touch the community in so many ways."I'm amazed at all that you do," Curtis said. "It's a spirit of service before self." -- email@example.com/221-3374
Quincy Alumni Association 2013
Sponsored By QPS Foundation


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Life is a journey. The destination is death. This blog is all about the musings of a sojourner in her thirties, curious about the stops, the fellow passengers, the driver(s), the conditions of travel and the highlights and lowlights. All the while in a place of tranquility: the sanctuary.
Saturday, April 6, 2013
The Paradox Pool
It is said that exceptions prove the rule.
So naturally, I am always in search of that elusive exception that makes the (opposite) point about the rule. :-)
Another guilty pleasure of mine.
A paradox, however is to a concept what an exception is to a rule.
Paradoxes fascinate me in a way that I doubt is normal, but my little deviance aside, I think the SMP is a haven for paradox-seekers like me.
In fact, on this very blog, I noticed a time when not a day went by without someone pulling a paradox out of the bag. It was astounding as to their frequency.
What I find particularly intriguing about a paradox is that it turns a complicated issue on its head and suddenly makes it extremely simple.
In the SMP, everything and nothing is complicated. Much like life itself, it has no meaning unless a meaning of it is sought.
In the post about 'masculine women', the oxymoron was not lost on me, but it was absolutely apt to describe certain very feminine women this way. Women thus described may not see this description of them as a compliment, but in fact, it is.
The paradox here is that optimal femininity requires some strongly masculine qualities. It is up to individual women to pick which masculine qualities suit their particular brand of femininity, of course. But the principle is a sound one, I think.
A familiar paradox to most of us would be, 'To be happy, one must be fully prepared to be...unhappy'.
This is definitely one paradox I have never really understood. But I am at least pondering it, which is arguably a step in the right direction.
If at least two-thirds of marriages collapse because one party is nothing more than unhappy, then we need to circumnavigate the goal-posts of 'happy' somehow, no?
How do we define 'happy'?
I refuse to look in the dictionary for this one. :-)
Part of the reason for this is...I wonder if the right word is being used in this context?
Would 'content' be a better word?
Would 'satisfied' do?
Some (wise) people define 'love' as a verb and not a noun.
Perhaps 'happy' should also be a verb and not an adjective?
As in, on waking up we make a concerted effort to 'happify' ourselves whatever the subsequent outcome of the day?
I wonder if this is feasible on a consistent basis...
Another important paradox I have come across is this one:
'In order to win, one must lose.'
Similar in principle to the biblical, 'The first shall be the last', this one is directly applicable to all aspects of life.
In order to enjoy harmonious relationships with others, one must be selfless.
In order to achieve the fulfilment of goals and dreams, one must demonstrate and exert self-control.
In order to gain it all, one must be prepared to lose it all.
These last two are especially important for women, I think.
The interpretation of a paradox is very much open. So, in this sense, it can get very simple.
Because it is essentially a case of 'one can make it whatever one wants it to be'.
Which is a great way to simplify life. :-)
There must be SMP-specific paradoxes out there. Anyone care to remind me?
13 comments:
- dannyfrom504 said...
yes. you have an EPIC hamster. but i adore you regardless.
- April 8, 2013 at 1:55 AM
- Spacetraveller said...
As I examine the gold medals that my hamster won in the Hamster Olympics, I say to myself...'Hm, this is one case where women could safely say to men 'Mine is bigger than yours' !
My indestructible, huge furryball of a hamster is a legend...in its own...lunchtime.
Hey, I adore you back...you know that.
Can you think of any more paradoxes in the SMP?
I thought of one which concerns Game...
Time permitting, I may elaborate on that one in a few days' time...
- April 8, 2013 at 7:01 AM
The biggest paradox in the SMP? The duality man/woman. The woman search something in a man(confidence/ security/ strength), the man something else(beauty/ agreeableness/ submission). Both believe they must present what they want in the other to have success, when it's a really bad idea. Thus we get the supplicating metro-sexual and the empowered woman. Both who can't find someone.
At the same time you have the dark triad man and the slave woman who get together for a time, then complain of pump and dump, single mother or not finding someone they can respect.
Human are funny creature isn't it?
- April 8, 2013 at 5:15 PM
- metak said...
What is cute small furry and has more medals than North Korean generals? ;-)
"There must be SMP-specific paradoxes out there. Anyone care to remind me?"
'The first shall be the last' -> Nice guys finish last.
'In order to win, one must not play the game.' -> MGTOW 101
I've got my own hamster you know... ;-)
- April 8, 2013 at 6:04 PM
- dannyfrom504 said...
paradoxes keep the scene live and flowing.
- April 9, 2013 at 2:52 AM
- Spacetraveller said...
@ Lifeuniverse42,
You are so right!
It is perhaps not too difficult to understand how the 'Strong and Independent thing' came about - if the perception was that women were enslaved by men, then the 'solution' to 'empower' women is actually logical. So in this sense, it was the original perception which was wrong, which therefore made the attempted solution also wrong.
But more importantly, for the life of me, I can't figure out why this strategy wasn't ditched as soon as it became crystal clear that it wasn't working.
But yes, great paradox! Thanks for that one.
Good one!
My hamster loves it :-)
The paradox you mention is a very painful one.
(For both men and women).
Yes, absolutely! It really adds 'spice' to the dating scene.
A lot of sensible dating advice goes something like, "You should be THIS, and yet be THAT..." which sounds really confusing to the uninitiated, but actually makes sense on close analysis...
- April 9, 2013 at 12:32 PM
- danny said...
dude, i post openly for a reason. i don't talk theory, studies, statistics, or any other crap.
i post about actual interactions i have with woman. success or failure. techniques i've used IRL, that have worked.
women really aren't that difficult to attract.
- April 9, 2013 at 7:29 PM
- Spacetraveller said...
True dat.
- April 10, 2013 at 4:33 AM
- Spacetraveller said...
What I meant was that:
'The first shall be the last' -> Nice guys finish last = painful for men.
'In order to win, one must not play the game.' -> MGTOW 101 = painful for women.
'Ouch' in both cases :-(
- April 10, 2013 at 5:53 AM
- metak said...
I perceive both options as bad in the long run for men and women.
Pyrrhic victory comes to mind.
- April 10, 2013 at 9:10 AM
- Spacetraveller said...
Look at it this way: in both cases, men feel 'aggrieved'.
If women are SOCIETY and men are CIVILISATION, then aggrieved men will cause both a downward-spiralling society and a crumbling civilsation.
Because aggrieved men refuse to be part of society (men are always on the 'edge' of society anyway, as societal rules are dominated by women, even in Patriarchal times) which causes lawlessness among women (perhaps in our times the lawlessness preceded the aggrievedness??).
If men feel hard done by, by society then there is no more incentive to maintain civilisation (which is dominated by them). So civilisation breaks down too.
With a crumbling economy following swiftly on the heels of a broken (Western) society, perhaps we have both scenarios in full swing already, as you might agree.
Question is, is there a way back?
Nothing is impossible, but maybe the worst is yet to come, followed swiftly by 'Recovery'.
- April 11, 2013 at 1:39 AM
- metak said...
Paradox? ;-)
Apparently women felt 'aggrieved' few decades ago and 'went their own way' so to speak. Now we have men felling 'aggrieved' and GTOW.
I look at things from a slightly different perspective. I don't see this society as 'women' and civilization (civilized isolation ;-) as 'men'. What we have is a sick society and f***ed up civilization. Pointing finger at women or men is a waste of time. It's clear that men and women (99,99% of population) get screwed. So who profits? There's one class that's only getting richer and richer from exploiting and creating this 'gender war'.
"The few own the many because they possess the means of livelihood of all ... The country is governed for the richest, for the corporations, the bankers, the land speculators, and for the exploiters of labor. The majority of mankind are working people. So long as their fair demands - the ownership and control of their livelihoods - are set at naught, we can have neither men's rights nor women's rights. The majority of mankind is ground down by industrial oppression in order that the small remnant may live in ease." Helen Keller, 1911
"Question is, is there a way back?"
Nope. We can only go forward. Things weren't perfect back then either. Now I see a good opportunity to come together and co-create something new that is based on those 'simple' things. Basic human dignity, helping each-other instead of competing or fighting, creating a new model of economy where men and women don't waste their lives slaving away only to provide for their families... etc. The Future's So Bright, I Gotta Wear Shades.. ;-)
- April 11, 2013 at 11:23 AM
- Spacetraveller said...
Hey Metak,
That last paragraph was beautiful. That's my UTOPIC ideal too :-)
And the real tragedy is, it is so simple to achieve if individual men and women adopt the right attitude.
But the alternative attitude (what we currently see everyday) is so much more tempting...
In the short term, that is...
If only we all thought a little bit more 'longterm', we would all be happier, I think...
- April 13, 2013 at 9:06 AM

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text: In a recent video, someone flew a drone over a group of geese in the park. You can see that the animals are getting irritated by having this drone fly over them. Finally, one of the geese flies unexpectedly at the drone, knocking it out of the sky where it landed upside down. The reaction to the video was unanimous by those who saw the video. It was not positive towards the pilot. Drone spying has certainly come in the news with more frequency. Television personality Mike Rowe has claimed to have shot one down as it flew outside of his bedroom. Yet, drone spying is not what it all seems. In fact, there are five reasons why drone spying is not what it all seems.


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Slake is an assassin used to getting what he wants, and what he wants is Raze. But he also wants to earn back the soul he sold when he was a much different demon. All he has to do is capture a runaway succubus named Fayle and hand her over to her family. What he doesn't count on is being caught himself by a web of lies—and his attraction to Raze.
Raze and Slake must navigate a dangerous world to be together. But as Fayle's jealousy of their relationship turns deadly, they find themselves embroiled in a battle not only for their love, but their lives and souls.
Comment: Just a short comment on a short story. This is a novella in the Demonica world, a series many readers have loved and still do.
In this story we meet Raze, an incubus demon who has a partner, Fayle, who helps him during his sexual needs and he helps her in return but one day Slake, another demon, shows up suddenly and although Raze doesn't have an idea why, he immediately feels attracted to him. Raze nothing will come out of it because incubus demons need a female sexual partner otherwise there will be pain. But when things get weird and difficult because Fayle wants to disappear but Raze has found friends and a god job and apparently Slake. But what would happen when Raze realizes Slake is not who he says he is?
I liked this story simply by the original pairing. It's the first m/m pairing with protagonist status in the series and it was great to see a different way of doing things, at least in the romance department.
It was great too to be able to see how characters we liked knowing about before were here giving a helping hand or being key to solve certain situations. Everyone feels like family and that's probably one of the best things about the series.
The plot isn't too far from things we've seen, Slake is looking for Fayle because it's part of his job in an attempt to finish a contract that will give him his soul back but then he falls in love with Raze and realizes he's more important. Raze himself also has some things to confess and at a time where he never thought things could go well for him, Slake is able to help and that changes everything. I thought it genius how Slake and raze were able to be a couple in all ways. That is the real surprise in the book and it suited all the things necessary. Quite the ingenious twist.
I was happy with the ways this happened. A full boom wouldn't have been bad, but I get the author's idea. Still, a great novella and a great way to "solve" some secondary character's situations.
Grade: 8/10


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Have you heard about the unbelievable William Hill Bingo October bingo special? Let us break it down for you because you might not believe it at first.
In a nutshell, every day in October, William Hill Bingo will be giving away Fiat Panda or Fiat 500 (the winner's choice).
That's 31 cars in 31 days. This has to be the boldest online bingo promo to date.
OMG, William Hill Bingo Is Giving Away 31 Cars in October
Will Hill Bingo is giving you a couple of ways to put yourself in the driver seat with this promo. Each day, you have two ways to earn a ticket into the draw and you can earn multiple entries to better your odds.
1. Pick up points to earn entries
Every time you buy bingo tickets and play side games with cash spend you'll earn points.
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2. Call on ball number 61
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From our friend Bill Brown in the Law School:
It occurred to me that some of the students might be interested in a class I am teaching next semester. Mergers & Acquisitions: Venture Capital, Private Equity and Entrepreneurship. http://www.law.duke.edu/curriculum/courseinfo/course?id=247 It is MW 1:30-2:50. We will use case studies that will focus on the business, finance, tax, corporate and IP matters relating to
--VC rounds
--Buy-outs of mature companies/divisions
--Roll-ups (consolidating fragmented industries)
--Financing over-leveraged/troubled companies
--Exit strategies
--Management compensation
Syllabus from last year is attached.
I currently have a couple of Fuqua students enrolled and would welcome other interested students.
Again, congratulations!
Best regards,
Bill Brown
Science Drive and Towerview Road
Box 90360
Durham, NC 27708-0360
919 613-8517
Howie Rhee, MBA
Managing Director
Center for Entrepreneurship and Innovation
Fuqua School of Business, Duke University
Room E167J
919-617-1123 mobile
Duke Entrepreneurship Links:
- Entrepreneurship at Duke - www.EntrepreneurshipAtDuke.com
- A Duke Resource for Entrepreneurs: www.dukeVEN.com
- Weekly Education Series - www.dukeDEES.com
- Connect with Duke Entrepreneurs - www.dukegen.com
- Find a startup - www.startupmatchmaker.blogspot.com
- Duke Start-Up Challenge - www.dukestartupchallenge.org
- Entrepreneurship and Venture Capital Club - www.dukeevcc.com
- Twitter - http://twitter.com/EshipAtDuke
- Entering the Duke Start-Up Challenge? See example business plans - email me for a copy of Business Plan Pro (advert)


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Protecture webinar on GDPR
GPDR webinar
This is advance notice that EAPA and Protecture shall be running a webinar on one of the issues which concerns us all at present, GDPR. The date is 11 April at 10.00 am
Information on this will be sent out in the next few days, but the purpose of this note is to ensure that you receive this advance notice for your diaries.
The session will be run by Gary Shipsey of Protecture. This organisation is a specialist consultancy rather than a legal firm and provides strategic advice on the objectives behind and the pitfalls of Data Protection. His focus is the understanding of what is important for users and holders of sensitive information.
Details of the webinar and how to join will be sent out shortly. The webinar will be hosted by Protecture. I am not sure how many will be permitted to join at this stage: clearly we are trying to maximise numbers, but please be prepared to book promptly to secure your place.


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@2019-20 www.brightsideglobaltrade.com
Brightside Global Trade JournalNew York, NY 10001United email@example.com
Although every effort has been made to ensure accuracy of published materials. We cannot accept any liability for omissions or errors in the information supplied for published. (c) 2017 BMSI. All rights reserved.
The full contents of Brightside Global Trade are protected by copyright. No part may be reproduced, stored in retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, or recording or otherwise, without the prior permission of the publisher.
Brightside Global Trade Journal is published in America. A free online magazine to keep entrepreneurs informed about business, legislature and policy.


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Please click on the image below for free online access to the Summer 2014 edition of Atlantic Beef and Sheep. Thank-you to our friends at DVL Publishing for making the online version of this popular magazine available.
Comments are closed.
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Fax: 902-893-7063
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Free "1000 Java Tips" eBook is here! It is huge collection of big and small Java
programming articles and tips. Please take your copy here.
Take your copy of free "Java Technology Screensaver"!.
JavaFAQ Home » General Java
User-defined class libraries also defined by the CLASSPATH variable.
Open Command prompt in Windows
and type: echo %CLASSPATH%
In Linux, Unix or MAC OSX in any shell: echo $CLASSPATH
The dot, .,
represents the current directory. The symbol [bin] stands for the
absolute path to the jdk1.1.x/bin directory. Therefore, if you keep
the bin and lib directories at the same directory
level, the Java executables will find the JDK classes (contained in the
classes.zip file). Note that the default class path also includes a path
to a classes directory on the same directory level as bin
and lib. You can put your own (unzipped) class files in a
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unsetenv CLASSPATH
You need unset if your CLASSPATH environment variable has been set to a value that is not correct, or if your startup file or script is setting an incorrect path.
RSS feed Java FAQ News


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- Country United States
- Alpha 10.0% —14.0%
This cultivar with its pale green bine was bred in 1968 with its sister Galena from Brewers Gold with an open pollination. It was released in 1979 in Idaho, one of the three hop cultivation areas on America's West Coast It is strongly "catty" flavored bittering hop with an alpha acid content ranging from 10 to 13% and used often in wheat beers. Substitutes: Galena, Nugget, Olympic.


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Best beach just because we ride our bikes here every Saturday.
Vote: BEST Beach
This is a close to everything large beach, no drinking or smoking on the beach so great for families.
Vote: BEST Beach
I love Mission beach because on one side you have ocean waves, and the other side has a nice calm bay!
Vote: BEST Beach
fun in the sun
Vote: BEST Beach
The 2017 San Diego A-List awards are based on more than 37,000 votes from local San Diego-area experts.
Best Greek ⋅ Best Wings ⋅ Best Sports Bar ⋅ Best Festival ⋅ Best Car Wash


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I learned several valuable lessons when it comes to developing your stand-up routine and quickly realized how these lessons applied in the world of data analytics.Read More
At times, it can seem as though the world of data visualization is the wild west, so we wanted to share some key takeaways, and best practices to help you improve your complex data visualization skills.Read More
Dating sites use algorithms in order to match potential partners. If you've ever tried online dating, then you've most likely benefited from the matchmaking powers of an algorithm.Read More
It's safe to say that data is everywhere. No matter who you are, where you live, what you do for a living, you interact with and consume data on a daily basis.Read More


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A malicious software program known as Conficker that many feared would wreak havoc on April 1 is slowly being activated, weeks after being dismissed as a false alarm, security experts said. Conficker, also known as Downadup or Kido, is quietly turning thousands of personal computers into servers of e-mail spam and installing spyware, they said. The worm started spreading late last year, infecting millions of computers and turning them into "slaves" that respond to commands sent from a remote server that effectively controls an army of computers known as a botnet.
- Is my computer infected with the Conficker worm? Probably not. Microsoft released a security update in October 2008 (MS 08-067) to protect against Conficker. If your computer is up-to-date with the latest security updates and your antivirus software is also up-to-date, you probably don't have the Conficker worm. If you are still worried about Conficker, follow these steps:
- 1. Go to http://update.microsoft.com/microsoftupdate to verify your settings and check for updates.
- 2. If you can't access http://update.microsoft.com/microsoftupdate, go to http://safety.live.com and scan your system.
- 3. If you can't go to http://safety.live.com, contact support at 1-866-PCSafety or 1-866-727-2338. This phone number is for virus and other security-related support. It is available 24 hours a day for the U.S. and Canada. For support in other countries, visit the Worldwide computer security information page.
- What does the Conficker worm do? To date, security researchers have discovered the following variants of the worm in the wild.
- Win32/Conficker.A was reported to Microsoft on November 21, 2008.
- Win32/Conficker.B was reported to Microsoft on December 29, 2008.
- Win32/Conficker.C was reported to Microsoft on February 20, 2009.
- Win32/Conficker.D was reported to Microsoft on March 4, 2009.
- Win32/Conficker.E was reported to Microsoft on April 8, 2009.
- Win32/Conficker.B might spread through file sharing and via removable drives, such as USB drives (also known as thumb drives).
- The worm adds a file to the removable drive so that when the drive is used, the AutoPlay dialog box will show one additional option. The Conficker worm can also disable important services on your computer. In the screenshot of the Autoplay dialog box below, the option Open folder to view files — Publisher not specified was added by the worm.
- The highlighted option — Open folder to view files — using Windows Explorer is the option that Windows provides and the option you should use. How does the Conficker worm work? Here's an illustration of how the Conficker worm works. How do I remove the Conficker worm? If your computer is infected with the Conficker worm, you may be unable to download certain security products, such as the Microsoft Malicious Software Removal Tool or you may be unable to access certain Web sites, such as Microsoft Update. If you can't access those tools, try using the Windows Live safety scanner.
- Where can I find more technical information about the Conficker worm and how can I stay up to date on the Conficker worm?
- • For additional information, see Centralized Information About the Conficker Worm.
- • For more technical information about the Conficker worm, see the Microsoft Malware Protection Center Virus Encyclopedia.
- • Bookmark the Microsoft Malware Protection Center portal and the Microsoft Malware Protection Center blog for updated information.
- • For symptoms and detailed information about how to remove the Conficker worm, see Help and Support: Virus alert about the Conficker Worm.
- • To continue to get updated information on security, sign up for the Microsoft Security for Home Computer Users newsletter. For more information, see How to prevent computer worms and How to remove computer worms. Its unidentified creators started using those machines for criminal purposes in recent weeks by loading more malicious software onto a small percentage of computers under their control, said Vincent Weafer, a vice president with Symantec Security Response, the research arm of the world's largest security software maker, Symantec Corp. He said that while he believes the number of infected machines that have become active is relatively small, he expects a consistent stream of attacks to follow, with other types of malware distributed by Conficker's authors. "Expect this to be long-term, slowly changing," Weafer said of the worm. "It's not going to be fast, aggressive." Conficker installs a second virus, known as Waledac, that sends out e-mail spam without knowledge of the PC's owner, along with a fake anti-spyware program, Weafer said. Related Stories • Conficker Worm Hits University of Utah Computers
- • Conficker Reportedly Updates Itself • Feared PC Virus Activates Harmlessly — So Far
- • How to Protect Yourself From the Conficker Virus • PC Virus 'Time Bomb' Set to Go Off at Any Moment • Computer Virus 'Time Bomb' Could Go Off April 1 The Waledac virus recruits the PCs into a second botnet that has existed for several years and specializes in distributing e-mail spam. Conficker also carries a third virus that warns users their PCs are infected and offers them a fake anti-virus program, Spyware Protect 2009 for $49.95, according to Russian-based security researcher Kaspersky Lab. If they buy it, their credit card information is stolen and the virus downloads even more malicious software. "This is probably one of the most sophisticated botnets on the planet. The guys behind this are very professional. They absolutely know what they are doing," said Paul Ferguson, a senior researcher with Trend Micro Inc, the world's third-largest security software maker. He said Conficker's authors likely installed a spam engine and another malicious software program on tens of thousands of computers since April 7. He said the worm will stop distributing the software on infected PCs on May 3 but more attacks will likely follow. "We expect to see a different component or a whole new twist to the way this botnet does business," said Ferguson, a member of The Conficker Working Group, an international alliance of companies fighting the worm. Researchers had feared the network controlled by the Conficker worm might be deployed on April 1 since the worm surfaced last year because it was programed to increase communication attempts from that date. The security industry formed the task force to fight the worm, bringing widespread attention that experts said probably scared off the criminals who command the slave computers. The task force initially thwarted the worm using the Internet's traffic control system to block access to servers that control the slave computers. Viruses that turn PCs into slaves exploit weaknesses in Microsoft's Windows operating system. The Conficker worm is especially tricky because it can evade corporate firewalls by passing from an infected machine onto a USB memory stick, then onto another PC. The Conficker botnet is one of many such networks controlled by syndicates that authorities believe are based in eastern Europe, Southeast Asia, China and Latin America.
This article is from Foxnews.com April 27, 2009
Microsoft information was also obtained from the foxnews.com article


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The Amazon Echo is the first smart consumer electronics product since the smartphone that's poised to become a daily habit for millions.
There are lots of interesting things to think about when it comes to Amazon's Echo device and the Alexa service that powers it.
Echo works because people like and trust Amazon. Because Amazon is truly a mainstream consumer brand, there's no reluctance to purchase the product, and very little resistance to trusting it to be reliable and useful.
Similar to Kindle, it's it's almost seen as not really being high-tech; iPhones are all about ultra-high-resolution Retina screens but Echo doesn't even have a screen.
Echo as a product doesn't have a category name. Maybe it's technically a "smart speaker" or something like that, but that misses the entire point about its utility and the connected services that power it.
If no category name emerges, that will bode well for Amazon, who might well become synonymous with the product category.
Read more: Amazon Echo and Alexa really matter — Medium
Don't forget to share this via Twitter, Google+, Pinterest, LinkedIn, , , BufferTumblr, Reddit, StumbleUpon and Delicious.


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UPDATE @ 7:47 p.m.:
Crews have cleared the large fallen tree limb from the road.
Firefighters on scene said they think the cause was weather related.
A large tree limb that had fallen on power lines has caused the 900 block of Spring Valley-Alpha Road to close in Spring Valley Township.
River levels rise in spots from heavy rain
Spring Valley Township Fire Department and Ohio State Highway Patrol was on scene of the fallen tree Sunday around 6:30 p.m.
Crews could not work to remove the tree until DP&L arrived to shut off the power lines.
It's not known if weather caused the tree limb to fall.


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The biggest, loudest, comfiest Bluetooth headphones we've ever found at this price. 30-foot wireless range, "hours" of battery life on a charge, "custom-shaped" neodymium drivers, built-in mic and call/media controls. They're a little light on the spec info, but they sound pretty good to us – especially for, like we said, 14 bucks
Model: BT8875M (a no-frills model number for a no-nonsense pair of headphones: BT for Bluetooth, four manageable digits, and an M, maybe for microphone, plus it wins Google – nice work all around).
Compare on Amazon at $31!
This deal is expected to expire at 1:59am on Thursday January 12th, 2017.


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Blended Biomass allows more Bagasse as Feedstock for Furfural! A report on blended biomass for Cogeneration, by Amaury Perez Sanchez: Cuba, like other countries with few
Tag: Cogeneration
Bagasse and Blended Biomass Cogeneration Advances (Cuban Example)
Cuba, like other countries with few fossil energy resources, has used biomass to fuel its industrial processes for decades. More recently, it has worked to


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I'm interested in speaking with an Investment Manager Research Team Member
I'm interested in learning more as a Prospective Client
Ellen Ogan Martel, Senior Consultant and Defined Contribution Practice Leader, will speak on a panel during P&I's 401(k)/403(b) Investment Lineup Conference. The panel, 'Measuring the Impact on Participants for Better Outcomes' will examine a variety of metrics that can be used to provide valuable insights for influencing participant behavior and assist them in reaching their long-term goals.
For more information on the conference, click here.


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You are welcome to email me directly here:
I can get letters and other mail here:
[I am moving in March 2019. I will update a PO box here once I have it.]
For publicity requests, please contact my publicist at Algonquin:
Brittani Hilles
Algonquin Books & Algonquin Young Readers
Email my publicist: firstname.lastname@example.com
For rights inquiries and blurb requests, please contact my literary agent:
Michael Bourret
Dystel, Goderich & Bourret LLC
Email my agent: firstname.lastname@example.com
Blurb policy: All blurb requests must go first to my agent, Michael Bourret. You can email the request to him directly at the email address above, and he will get an answer quicker than if you email me. However, please note: I am currently closed to all blurb requests until Feb. 1, 2019.


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Hallo Bloglanders
I hope you are having a Marvellous Monday. TODAY I'm implementing all my new year's resolutions but more about that in another post.
Over the weekends I normally have time to "gather" ...................... information, ideas, things I see in my garden, town, shops or online. So, I'll be bringing you something that I've "gathered" each Monday.
First up:
Wax Highlighters!! I totally hate the normal ones - they make a squeaky sound that reminds me of university so I was very glad to get my hands on these wax ones. Best part - they won't dry out! Also, they are rather affordable and it's readily available even down under in Sunny SA. Mine is from Bostik, they come in a pack of 5 - orange, blue, pink, yellow and green.
That's it. I'm off to my craft room to do some serious crafting. I'll be back to tell you all about those New Year's resolutions. Watch this space!
Thanks for looking at my blog and enjoy your day.


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The sun is out and shining, I'm not looking at snow, and it's Friday so I'm pretty freakin psyched for today!
Continuing with the spirit of National Nutrition Month, the weekly wrap up is going to be a little different today. This post is all about giving you resources to educate yourself this month about proper foods to eat and how to live healthy all while being body positive! It's important to keep your health and happiness in prospective. All of the content that is out on nutrition, dieting, calories, and so many other topics can just get overwhelming and drive a person crazy. Below are just a few tools you can use to exercise, cook, and read that you don't need a PhD in nutrition to understand.
Choose My Plate– this site is by far the easiest tool I have discovered so far. When I was studying to become a mentor for Get Fruved (yesterday's Spotlight Thursday) I had no idea what health was, looked like, or how I was going to talk to others about it. Choose my plate is literally a one-stop-shop site where you can find information on health as well as tools that are easy to use for beginners. You can find recipes, info in portion sizes, BMI calculators, checklist and so much more to help you on your healthy journey. And for an extra awesome point, they provide quizzes to test your knowledge on healthy facts and habits. Maybe quizzes only get me excited because I'm a nerd but still, everything you could need or want is here for you. This in my number 1 recommendation for all.
Food.com Beginner Cook– So I'm a true foodie. When it comes to food I am there with bells ringing but this was also my downfall when I tried dieting in the past. I am a firm believer that just because you want to eat healthy that flavor has to be sacrificed. I make no apologies for not want to eat plain steamed broccoli in the name of health so I found this great site at food.com that has great flavorful food but is also healthy. They provide the list of ingredients, cooking time, and a picture of how it's supposed to look when you're done. I like this site because it also has vegetarian recipes but it is lacking in paleo and vegan options but I'm sure a seasoned veteran can make these recipes suite their vegan or paleo needs. Pinterest is an easier option if you are a beginner in the paleo or vegan lifestyle. Just look up clean eating and you will find tons of articles and recipe options.
Hulu Plus– so Hulu is shaping up to be useful in more ways than just watching TV shows. The latest edition to the mega streaming platform are exercise videos. Hulu just recently partnered with the makers of P90X (that super intense exercise program. You know the one I'm talking about) to bring personal training into your home. New videos are loaded every morning for you to do in the comfort of your home. This means you don't have to go to the gym, put money down on a gym membership, or exercise next to the half naked girl or guy making strange grunt noises. Hulu is $7.99 a month and if you're already a member you can get a 30 day free trail so it's at least worth it to try and if you don't like it, it only cost you 8 bucks which isn't bad compared to what you could have spent on a membership and you only used that membership twice.


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Ariana Pharma and Voisin Life Sciences Consulting present a conference in Boston on November 12th addressing the topic:
Product Development 'What the FDA's Innovative Initiatives enable'
Modern approaches for managing the complexity of product development
Ariana Pharma and Voisin Consulting Life Science are happy to host a seminar by Frederico Goodsaid, PhD – VP, Strategic Regulatory Intelligence at Vertex Pharmaceuticals and Antoinette Paone, MS – Sr Director, Head of Regulatory Affairs CMC at Vertex, who will discuss in detail the story of the development of Kalydeco.
This presentation will be hosted by Mohammad Afshar, CEO of Ariana, and Mark A. De Rosch, PhD – VP, regulatory Drugs/ Biologics at Voisin.
Please note that attendance to this conference is by invitation only. If you would like to join us, please contact Chad Mills.


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SHIVIKA- PYAAR KI DASTAAN episode 38 (ishqbaaz ff)
Hi, am here with the 38th epi. Sorry guys for short ones but 40th one will be long, pakka, pinky promise.
Epi 38-
At the mall-
Shivika leave towards a different shop for household items with anika's hands wrapped around shivaay's arm. Ishana found their proximity a bit awkward as according to what anika had told her they were "just friends".
Ishana- omkara, don't u find taai and shivaay's "friendship" a bit more than what it is?
Om(thinking)- looks like anika has not yet informed her about her relationship with shivaay. It will be better if Ishana gets to know about them through anika only and not any third person.
Om- Ishana! We have come here for furniture not shivika
Ishu- shivika??
Om- uffho!! Just come!!
He drags away Ishana with him.
In the train-
Saumya had a lower side birth and rudra had gotten the upper one. Everyone were sitting and chatting or listening to music or watching a movie.
Rudra- hey guys come on! This is not fun, lets all play a game
(I have used some random names and it is completely fine if u don't remember all the names, there will be just two or three who will play a short cupid role and u will understand who as the story proceeds)
Jeevitesh- good idea. But not the same ol' truth n dare
Richa- not even antakshari
Sneha- and no dumb charades too
Rudra- ummm… hey! How about 'never have I ever'?
Jiya- ooooo… that's my favorite babes
Rudra- really? That has become my favorite too now (flirting)
Saumya (in her mind)- god!! I thought he is a seedha-sadha boy, but he is such a flirt!! He has not left a single chance to flirt that jiya ever since the journey started. And this jiya! She always is behind flirty boys right from school days!!
(so jiya was saumya's school mate and now college mate, they r not friends)
Rudra- hey saumya! U too will play right?
Saumya- yea…
Jiya- she? Hmph!!… this fatso will play with us??
Saumya- oh shut up jiya!! i will play whatever I want
Jiya- hey! I don't have any probs but then u will not be able to continue till the end
Rudra- jiya, why do u say so?
Jiya- rudra, I know her since school days, she is such a bore, she has nothing adventurous.
Jeevitesh- that is ok, saumya will play
Back at the mall-
Shivika are sitting on chairs with a variety of utensils placed before them
Anika and shivaay are shopping for utensils.
Anika- no shivaay, not this, not this, not this, none of these!!
Shivaay- then what??
Anika- arre!! We r not buying clothes that you will spread all these in front of me and tell me to choose, we r shopping for utensils!!
Shivaay- what's the difference??
Anika facepalmed herself
Anika- buddhu singh oberoi! Come with me and learn how u have fun and shop and manage time and money
Anika takes shivaay to the section where utensils were there. She pulls shivaay around wherever she goes.
Anika- ok, I have finally decided we will take the blue themed utensils
Shivaay- u keep themes for buying??
Anika- no and yes. many don't but I do
Shivaay- yeah, how could I forget, ajab gazab
Anika- did u say something
Shivaay- huh? No, nothing
Anika keeps picking up things and shivaay keeps adoring her. She starts making tunes and beats with the utensils and shivaay joins her too, they laugh, they chat, they play around.
While going through the different sections, anika comes across a variety of ice trays.
Anika- shivaay!! Look at these!!
Shivaay- what is so much in them to be excited about, they are the usual ice cube trays
Anika- urgh! I am not talking about ice "cube" trays but about ice "emoji" trays
Shivaay- emoji?? What are emojis??
Anika ( giving a bewildered look)- you do not know what are emojis??
Shivaay (casually)- no
Anika- rudra is right, u cannot be of young generation, even dadi knows emoji!
Shivaay gives her a whetever look. Anika shows him emojis on her phone and he understands
Shivaay- what is the need to call with such names? Cant they simply call it symbols ot something like that?
Anika- u just leave it! Buddhe (old man) kahike!
Shivaay- hey I am not a buddha (old man)! Ok! I don't understand such abbreviations!
Anika- seeing my situation I feeling like singing only one song
Shivaay- what? I mean which one?
Anika- main kya karoon raam mujhe buddha mil gaya
haay, haay buddha mil gaya
main gudiya haseen meri morani si chaal hai
sar men safed us ke daada ji sa baal hai
bigadega har kaam, mujhe buddha mil gaya
main kya karoon raam mujhe buddha mil gaya…..
Shivaay manages to shut her mouth finally and gives her death glares.
Shivaay- u got an old man?? Accha, now see, I wont even talk to u, leave alone talk I wont even look at you.
Anika- ooohhh nooo, I was just joking!
Shivaay (sarcastically)- hahaha it was such a good joke, look I am laughing!!
Anika- accha, ok fine, it was a bad joke but why are you taking it seriously? Huh! I thought u were touchy of only ur height and kaali coffe and saira banu, looks like u have another touchy topic—being old!!
Shivaay was enraged but then anika started to laugh her heart out and this sight made forget all his anger in moment and brought up a smile on his face.
Shivaay- done?? Now can we go?
Anika just nods yes still trying to control her laughter.
They see for kitchen and hall accessories and play with them having a gala time.
On the other hand, ishkara were looking for furniture
Ishana (giving out a damn tired sigh)- that's it! I am soooo tired!
Om (looking at her in a sarcastic way)- doing what?
Ishana- what? Arre! Ask what now! Walking around so many places from one corner of the shop to the other and then to another shops and then corner to corner of that shop too and then to other corners and hidden places of both the shops…
Om- done??! Or have u forgotten to say something else?
Ishana- haan! I forgot, walking to the centre of both the shops!!
Om- oh god!! I will go mad with u
Ishana- u r already mad, how much madder do plan to become??
Om- excuse me??!!!
Ishana- leave all of that. U want the shopping to get over soon right? And I want to relax right?
Om- yeah so?
Ishana- now we will do it in my style, Ishana style
Om- and how is that?
Ishana- wait and watch
She goes from there but then comes back in front of om
Ishana- and learn!
She goes to one of the places where the sofas and recliners were set perfectly like it is set at home. She goes and enquires about a few details from the boy standing there who works in the shop.
Ishana- hmm… lets see how comfy vomfy this is.
Om (in his mind)- comfy vomfy??!! This girl is really mad
Ishana then settles herself on the recliner and rests, she then gets up.
Ishu- yeah yeah, it was ok type, lets see other
Om gives her a u-r-impossible look
They then see a body massager chair and Ishana sits on it and asks the boy to turn it on so she could see how well it works. While Ishana was having a nice body massage, om was just looking at her.
Ishana- what happened mr. zulfi singh oberoi?? Want a massage?
She turns towards the boy
Ishana- can u let that lond hair man see how the other model works? We will take the best of the two
And so om was made to sit on the other one, first he resented but then he started liking it.
So they too shopped in Ishana's style.
After a long time of shopping shivika and ishkara decided to meet at the food court.
When Ishkara reached food court, shivika were already seated there chatting, laughing and eating.
Precap- tears run down saumy's cheeks and two finger wipe them gently (yup, it was rudra), they have a cute eye lock.
Guys sorry for short ones but then the 40th one will be long pakka, pinky promise.


text: >>>>> "Crispin" == Crispin Cowan <email@example.com> writes: Crispin> "Stephen J. Turnbull" wrote: >> I don't think anybody is good enough at producing software >> reliably to budget that more than a few firms can hope to >> survive long in the shrink-wrap market, period, and open source >> firms are at a disadvantage because they do not have a >> reasonably long "initial monopoly" period to build up reserves >> for the next "killer product." Crispin> This seems to predict exactly the opposite of observed Crispin> behavior (over 100 Linux distributions). I submit that Crispin> open source companies get to use the community's software Crispin> development labors, and thus focus on packaging issues. Crispin> As a result, open source companies need far less in the Crispin> way of resources to survive until the next killer Crispin> product. Sorry, I should be more clear. I don't consider a Linux distribution a product of software development. The product is (nearly) all packaging. Amazon.com knows nothing about publishing. Linux distributors need to know more than that about development, of course, but their core skills are (IMO) closer to Amazon's than to Sun's. Only a few will survive as pure free software businesses. Crispin> Or a strategy focussed on packaging, distribution, and Crispin> service. Explicitly abandoning development, I see. That's not what we want! We want people making _big wins_[1] for _creating_ and _developing_ products and contributing their source code to the community. Otherwise the clear virtues of open development will be used by proprietary firms with proprietary licenses (or even source escrow) or patents, and free software could end up being marginalized, or restricted to niches like OSes (a big niche, to be sure, but still a niche). Eg, AbiWord. I hear nothing about AbiWord on any of my LUG MLs; but lots about Star Office and Word Perfect, both proprietary software AFAIK, although distributed in free-beer forms as I understand it. Can AbiWord really compete? We'll see ... but I would bet against it becoming the kind of force in wordprocessing that Linux is in OSes. Footnotes: [1] Early retirement is big enough. -- University of Tsukuba Tennodai 1-1-1 Tsukuba 305-8573 JAPAN Institute of Policy and Planning Sciences Tel/fax: +81 (298) 53-5091 _________________ _________________ _________________ _________________ What are those straight lines for? "XEmacs rules."


text: How Severe Weather Can Lead to an AC Repair Posted August 11, 2017 Cooling While your air conditioner was designed to withstand all of the crazy weather in Stuart and West Palm Beach areas, there are times that severe weather can lead to an AC repair. For instance, bad weather oftentimes coincides with power surges. Since your AC is electronic, and power surges and electronics are not compatible, you'll want to take steps to protect your AC. It's most appropriate to turn your system off using your thermostat and turn off the circuit breaker to increase protection during stormy weather. Some people worry about too much water getting on their air conditioner and choose to cover it with a tarp or cover. This does not help, and could actually damage your AC if you forget to remove the covering before running it again. Your AC was designed to handle rain and snow, so keep your money and prevent a potential AC repair by not covering your unit. However, if you find your unit is in standing water after a storm, you'll want to call Arnold's Air Conditioning of South Florida Inc before you turn it back on. Another hazard to your AC throughout severe weather is falling (or flying) objects. This is a little difficult to prevent, but keeping loose outdoor items contained can help lower your risk. Once the storm passes, make sure to clean up any debris that may have gathered around your unit. If debris does hit and damage your unit, Arnold's Air Conditioning of South Florida Inc can check it and perform an AC repair if it's needed. In addition to storms, summer is known for its blazing heat. Just like it was made to withstand water, your AC was made to handle lots of heat from the sun. But, the hotter the sun, the harder your AC has to run to cool your house. You can help control some wear and tear by keeping your home's temperature setting a little bit higher on these days. The closer your indoor temperature is to the outdoor temperature, the less strenuous the work is for your AC. Many people are still comfortable with their thermostats set to about 78 degrees Fahrenheit on extremely hot days. Note that the older your AC is, the more it may struggle in hotter weather. If you have any questions about the threat of severe weather on your air conditioner, or think you are in need of an AC repair, call Arnold's Air Conditioning of South Florida Inc. We can be contacted at 772-205-3269. Back To Blog


text: This was the second time I had the honor of photographing Summer for some dance pictures and head shots. She's is one of the most outstanding dancers you will ever watch. I know this child will make a way for herself.. she is such a hard worker and all around sweet girl. If you would like to see her other session I shot in downtown Fayetteville click on the link here.


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Disposable Food Box Production Line
1. Working stations adopt four-pillar structure. --more stable and more precise.2. Touch screen, PLC and servo motor adopt Delta brand from Taiwan.3.Material holder combine with the machine.It can save workshop space,easy to control..Forming station adopts savro motor system,more stable and efficient.4.Forming station adopts savro motor system,more stable and efficient.5.Heaters adopts "Elstein" from Germany, vacuum insulation cotton--save more energy.Chat Now
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text:
Shi'ite rebels, who dissolved Yemen's parliament on Friday, 6 February, and took power in the country are drawing flak from Yeminis and neighbouring Gulf countries alike.
Thousands of protesters took to the streets against the rule of the Houthi rebels in capital Sanaa on Saturday, as some Gulf countries denounced their "coup".
On the other hand, Abdel-Malak al-Houthi, leader of the Houthi rebels, defended his move of dissolving the parliament after the group held the former president of the country captive, following which he resigned.
In a televised address to his supporters, Al-Houthi said: "This historic and responsible initiative is in the interest of the country... Because it fills a political vacuum."
"[It was] in the interest of all Yemenis without exception, including the separatists of southern Yemen," the new leader said.
Speaking about the presence of al-Qaeda, Al-Houthi said: "If al-Qaeda takes control of the country, it will plot against our brothers in Saudi Arabia and the Gulf."
While the unrest in the Arab world's poorest nation could benefit al-Qaeda which is active in the region, the Gulf Cooperation Council countries see the Houthi coup as an "escalation that cannot be accepted under any circumstances".
Six Arab countries of the Gulf Cooperation Council, led by Saudi Arabia and the UAE, issued a statement on Saturday calling for the UN Security Council to "put an end to this coup".
"The Houthi coup marks a grave and inacceptable escalation... and endangers the security, stability, sovereignty and territorial integrity of Yemen," the GCC headquarters in Saudi Arabia said, the Daily Star reported.
The Sunni-dominated GCC, which includes Bahrain, Kuwait, Oman, Qatar and the United Arab Emirates, had urged the Houthis to pull out of Sanaa, which the militia overran in September last year.


text:
Paging all Predators fans, it's time to mark up your calendars! Not only does everyone need to prepare for the Predators' FIFTEENTH season, but also the fact that it's an Olympic year!
Now let's bullet point this baby and check what the new season has in store.
* For anyone who trolls around the NHL site, the first news to bring up is the list of new names for the four restructured divisions. Good news for Predators fans – we are in the same conference and the same division as always: the Western Conference and the Central Division. We join the Chicago Blackhawks, Colorado Avalanche, Dallas Stars, Minnesota Wild, St. Louis Blues, and the Winnipeg Jets (who are finally out of the southeastern division) for a rough and tumble new season.
* Next important point: what is up with the pre-season? Well folks I hope you're looking forward to trips to Florida and New York for the pre-season, cause the Predators are heading to both. The Preds will head south to play the Florida Panthers in a double-header September 16th to kick off the pre-season, followed by ten days of eastern traveling to face down the Tampa Bay Lightning, New York Islanders, and drop by the Washington Capitals. For the full pre-season breakdown, head on over to the Predators official site and take a gander.
Whew, sounds like a busy time! Now onward to the regular season schedule.
* The important dates for next season for the Predators (at least in my opinion) of course include the season opener, October 3rd at the St. Louis Blues, and the home opener, October 8th versus the Minnesota Wild. After the season opening fun, we fans of Nashville get to see a full season of hockey up until April 13th when all Playoff positions will be filled.
* Hold tight to your seats Predators fans, it's time to look at those long home and away game stretches. The longest home stretch goes right through the Olympics, lasting six games from February 8th through March 8th. Alright, prepare yourselves. The longest away stretch is a seven game stretch from November 2nd through the 15th, starting out on the west coast before swinging east. Quick, go find what channel these games are on!
* As much as I love to watch every Predators game on the schedule and cheer on the rivalry games, there is always that set of games I want to keep an eye on. The team I have in mind doesn't really have much of a connection to Nashville at all. So what games could I possibly be writing about? Well that would the five games the Predators will play against their new division rival the Winnipeg Jets. Nashville has not had many opportunities to play this team since relocating to Winnipeg, not to mention not playing the Jets at all last season.
* My closing notes on the new schedule have to do with something that is not technically in the schedule at all. From February 9th through the 25th, there will be a break for the 2014 Winter Olympic Games in Sochi, Russia. Our boys in the NHL just got the go ahead today to play, so pick your team and spend those two weeks cheering on your country!


text:
The ability to give a persuasive presentation is a tremendous career booster. These powerpoint slides show you how to create and deliver powerful presentations to any group, of any size, for any purpose.
You can download these brilliant presentation slides HERE.
Free Download - Amazing Powerpoint Slides on Fundamentals of HR Management and HR Scorecard
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text:
Many years ago, I was also a single woman waiting for "the one". I have also been witness to single friends who are struggling with doubts about their self-esteem because no one seem to be interested in them. I ache with these single friends with their deep longings in their hearts. I always remember them during these times where everyone is celebrating togetherness as a couple, whether they are dating, engaged or married. In a world filled with mushy stuff this Valentine's day, I lift up my single friends. This poem I've written is for you.
THE LORD HEARS YOUR CRY By Leah Albano The heavens are witnesses To your every heartache. The angels know Of every tear that you shed. The prayers of your heart, Hoping for someone to love you, Pleading that a man Will come to care for you. All these are etched In God's mind. Know that He has you In His heart. The Lord hears your cry. The Lord knows of your pain. He understands your lonelines. He feels your every doubt. Know that He is there To take away your every tear. Know that He is there To soothe away your every hurt. Look unto Him And trust in His goodness. Let this period of waiting Be a period of growing intimacy with Him. God alone, in His infinite faithfulness Shall meet that void you are feeling. He alone, is the Lover Whom you will ever need. Written: February 13, 2002 Photo: CTTO


text:
Research Topics
Genomes and Genes
Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
Principal Investigator: Dieter A Wolf
Abstract: DESCRIPTION (provided by applicant): ): Bipolar disorder (BPD) is a neuropsychiatric condition defined by a lifetime of relapsing &remitting manic &depressive episodes. This mood disorder has been shown to have strong genetic linkage with familial predisposition. A major impediment to proper diagnosis has been such confounders as the prevalence of substance &alcohol abuse/dependence among those meeting criteria for BPD. Substance abuse, intoxication &withdrawal may elicit mood episodes that recapitulate bipolar phenotypes. Typically, at least 6-12 months of abstinence from substances is required to diagnose a mood episode as underlying BPD. Clinical practice often resorts to early psychotropic medication because of potentially extreme irritability &impulsive suicidal actions. Lithium has been the standard of treatment for BPD, but, because of its side effects, anti-convulsants (including valproic acid, lamotrigine, topiramate &carbamazepine) &atypical anti-psychotics have also been prescribed. The underlying etiology &mechanisms of disease therapy are poorly understood {Rosenberg, 2007 #160}. Adequate laboratory (including animal) models have been difficult to establish {Fornito, 2009 #161}. Microarray analyses have been performed on post-mortem brain samples &compared with controls &patients with schizophrenia, but no clear distinction between neural subtypes, glia, &surrounding vascular cells has been evident (Kim &Webster, 2008). There is no consensus on gene expression patterns linked to BPD &, hence, very little insight into underlying molecular mechanisms. Protein kinase pathways may be altered. Lithium alters MEK &ERK phosphorylation {Pardo, 2003 #162}, decreases CREB phosphorylation &activity of CaM kinase IV {Tardito, 2007 #163} in rat hippocampal neurons. Lithium &valproate may reduce phosphorylation of rat GluR1 {Du, 2008 #164}. Lithium correlates with reduced phosphorylation of the NMDA receptor subunit NR2B in rat cortical neurons {Hashimoto, 2002 #159}. Studies in rat brains have also suggested that lithium administration reduces translocation of Protein Kinase C from the cytosol to the cell membrane {Hahn, 1999 #158}, &inhibits GSK3 activity in mice (Catapano &Manji, 2008). Given the diversity of implicated kinase pathways in BPD and limited by obstacles in studying human neuropsychiatric diseases, we sought to develop a representative, predictive model system to explore regulation of protein phosphorylation in neural cells that most faithfully recapitulates underlying defects of actual human BPD. Recent advances have allowed the conversion of patient-specific human fibroblasts to human induced pluripotent stem cells (hIPSCs), cells which become sufficiently dedifferentiated to a primordial developmental stage that they now emulate human embryonic stem cells (hESCs) in terms of their ability to give rise to the 3 primitive embryonic germ layers and following various differentiation protocols, to more mature, tissue- and organ-specific cell types, including those in the neural lineage. Our group not only has the ability to generate hIPSCs from fibroblasts from normal individuals &from those carrying difficult-to-model diseases, but has actually done so for some neurogenetic/neuropsychiatric entities, e.g., Rett Syndrome, &has differentiated them to neural lineages. We propose to generate hIPSCs from a well-defined subset of BPD patients (i.e., the lithium-responsive subpopulation) in order to begin more faithfully modeling BPD from a rigorous molecular mechanistic perspective using material derived from actual patients. We will include control cell lines from unaffected patients as well as patients with neurologic or psychiatric disorders that are not BPD. At the core of beginning to understand the molecular basis of BPD - and an aspect for which hIPSCs would be particularly well-suited &informative - is a better understanding of changes in the expression of key proteins, particularly their phosphorylation state, at the level of the proteome &phosphoproteome. Our team has been particularly adept at using phosphoproteomic analysis of hESCs (the 1st such analysis in the field) to identify key (including novel) drugable signal transduction pathways that influence neural differentiation. We believe we can apply a similar approach to hIPSCs from BPD patients. In other words, we hypothesize that identification of proteomic &phosphoproteomic differences between hIPSC-derived neurons from BPD vs. unaffected controls or controls with other neuropsychiatric disorders will help elucidate pivotal, diagnostic, and potentially drugable molecular mechanisms underlying BPD. Multidimensional liquid chromatography (MDLC) tandem mass spectrometry (MS/MS) is a powerful tool for analysis of proteomes, phosphoproteomes, and is a strength of the Burnham Institute. MDLC-MS/MS will be used to analyze the proteomes and phosphoproteomes of normal and patient-derived hIPSCs and their neural derivatives. We will use refined bioinformatic tools (another Burnham strength) to glean critical differences among the cell types. We will quantify total cellular proteins, with a particular focus on site- specific phophorylation. This large-scale analysis will likely yield a number of candidate molecular differences between control &BPD cells, any of which might suggest improved methods of diagnosis &treatment. While we will be able to perform follow-up analyses on only a few proteins predicted to be key to control of pluripotency, neural differentiation and neural function, this valuable dataset will be made available to the broader research community so that complementary parallel studies may be launched on the basis of these proteomic &phosphoproteomic results. PUBLIC HEALTH RELEVANCE: Bipolar disorder (BPD) is a severe and prominent societal malady with poorly understood etiology. Proteomic and phosphoproteomic technology is a powerful analytical platform allowing unbiased identification of molecular profiles of healthy and diseased cells, e.g. those from normal and BPD patients. We propose to merge the application of induced pluripotent cells (iPSCs) from BPD patients with comprehensive proteomic/phosphoproteomic analyses of these iPSCs and their neural derivatives, to discover molecular underpinnings of the abnormalities in BPD patients, as well as potential targets for improved diagnosis and treatment.
Funding Period: -------------------- - --------------------
more information: NIH RePORT


text:
The controversial DNC funded dossier that is the basis of the still on-going Mueller investigation into possible coordination between the Trump Presidential Campaign and the Russian Government was blasted by the President in a Tweet sent out on Tuesday.
Republicans in Congress are currently investigating how heavily the Mueller investigation has relied on the dossier and whether they used it to justify a surveillance warrant against former Trump adviser Carter Page. A former British spy Christopher Steele produced the document, after being hired by the controversial Fusion GPS. Fusion GPS is an opposition research firm that got funding from both the Hillary Clinton Presidential campaign and the Democratic National Committee (DNC).
In a closed-door session with the House Intelligence Committee last week, FBI Deputy Director Andrew McCabe stood by the document's credibility according to Fox News, but when pressed to identify what information included in the dossier the bureau had corroborated, McCabe cited only the fact that Page had traveled to Moscow.
It seems that this yet unsubstantiated witchhunt will continue into the new year regardless of any solid evidence that merits the exercise.


text:
Hi! I'm Allie. I'm a 20 year old North Carolinian that's attempting to go vegan. These are my veggie chronicles…
I've always had a passion for food and health. I'm a huge believer in the power that a healthy, natural lifestyle and I hope become a kind, honest, eco-friendly, and healthy as I can. So why vegan, you ask?
For the animals: I believe that we can co-exist peacefully with animals without causing harm. I do not believe that it is necessary to consume animal products to be healthy, therefore I feel that slaughtering animals is unnecessary and cruel.
For the planet: We all know (I think… I hope!) that reducing meat intake reduces your carbon footprint. I believe it's important to cherish and respect our planet, and that we should always do what we can to protect it :).
For me: Beside keeping me morally and ethically sane, I have felt better since going vegan! I'm more energetic, have seen a natural decrease in overall body fat, quicker recovery time between hardcore workout, clearing of skin (not quite cleat yet, but getting there!), and just overall feeling better!
I believe that one person can make a difference. Stick to your ideals, hold on to your principles, keep your feet on the ground, and your eyes ahead. Get informed, change your habits, change your life :).


text:
My absolute favorite thing to blog about is any time I make a complete and utter fool of myself. Luckily for all of us, I have such a story!
Last night I went to the gym. Weirdly, this is how many of my good, ridiculous, Jennifer-is-an-idiot stories begin.
The gym I go to is a sort of boot camp with instructor- led, daily classes. The class on this particular night used TRX bands, which are bands that hang from a bar near the ceiling and look like this:


text:
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Walk-in testing services are offered for:
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- Fingerprinting
Venipuncture (blood draw) tests always require an appointment
Under the Federal Department of Transportation Regulations, these test types must be OBSERVED.
An appointment is required for the following services:
- Return to duty urine tests
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Make sure to read through the information on the Preparing for your Appointment page
Drug & Alcohol Testing 24/7 serves Wisconsin and the nation as a drug and alcohol program management solution and as a local collection site in Wisconsin.
As a Service Agent or Third Party Administrator we can offer the following services:
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Drug & Alcohol Testing 24/7 does not require signed contracts with any clients. We believe, as a client, if you are not happy and we are unable to meet your expectations, you should be free to change providers or collection sites without consequence.


text: Fishing up and down the reef the past couple of weeks has been very inconsistent, especially with the crazy weather we've had. The days that the conditions have been right, east wind and north current, the fishing has been great almost springtime like. Kings, tunas, some dolphin, and great snapper fishing. But we can't get the conditions to last for more than a day or two. We've had lots of south current and west wind which always pushes in cold water this time of year, while it's not always a bad thing, that cold water pushes in the deep water species, vermilions, pink porgies, snowy and warsaw groupers. When we are trying to catch kings and tunas it's the kiss of death.