text:
Kansas City planning apartment complex with low energy use
KANSAS CITY(AP) - Developers are planning a 276-unit apartment complex in Kansas City that they say will use only about 10 percent of the energy used in a comparable building.
The complex, called Second and Delaware, is scheduled for construction on the Missouri riverfront just north of downtown.
The Kansas City Star reports national experts say the $60 million project will be the largest U.S. multifamily apartment complex using construction certified by the Passive House Institute.
Construction will include 16-inch-thick walls that sandwich insulation between concrete panels. Proponents say the method saves large amounts of energy and is capable of withstanding all types of severe weather.
Excavation is scheduled to begin next month. If all goes as planned, tenants could begin moving in by February 2017.


text: "We have been inviting our international trade partners to Bremen for over 50 years. And each year we get to welcome so many guests. Over the years the BISS has not only established itself as an important event for maintaining trade relations, but also as a kind of family get-together. After all, lots of our partners have been coming here for many years, in part in the second or third generation," explains Weiss. The motto of this year's meeting was handling complexity, which was a common thread running through the event.

subset: commoncrawl


text:
Americans are gaining a much clearer view of the ongoing class struggle. National Jobs with Justice (JwJ) and other organizations are calling for a "People's Bailout" in opposition to the handouts flowing from the Bush Administration to the biggest bankers. Activists across the nation responded to JwJ's call for a week of action, December 7-13. North Texas students responded in the college town of Denton, an hour north of Dallas.
Twenty-five students and 6 JwJ activists from Dallas gathered in front of the Wells Fargo Bank in the middle of downtown. Organizer Stewart Minor kicked off the responsive chant, "I don't know but I've been told, C-E-O pockets are lined with gold!" A large percentage of the passing drivers honked their solidarity. The crowd sang "Which Side Are You On" before breaking into the standby, "The People United, Will Never Be Defeated" in English and Spanish, "El pueblo unido jamas sera vencido.".
Leaflets, which had already been distributed all over the University of North Texas, followed the wording suggested from National JwJ:
As many predicted, the Wall Street Bail-out has proven to be the gross give-away to the same financial bigwigs that have been pocketing millions while wrecking the real economy. Little or no benefit has gone to the working people and the real economy, at a time that we face the greatest economic crisis since the 1930s. By the time Obama is sworn in, hundreds of thousands of additional people will lose their jobs, lose their homes and lose their health care.
It's time for a "People's Bail-Out" that fixes the real economy, restores a voice for working people in challenging corporate greed, provides emergency help to the victims of the crisis and begins building a fair economy that works for all, addressing crises in housing, health care, jobs, retirement security and the environment.
Jobs with Justice coalitions and ally organizations around the country will take part in an emergency campaign over the next 2 ½ months to get Congress and the new administration to enact a Peoples Bail-Out.
Immediately we call for:
Pass a large economic stimulus/recovery package, on the scale of the emergency we face;
Pass the Employee Free Choice Act (EFCA);
Stop evictions due to foreclosures;
Emergency action so people losing jobs don't lose health care.
Lay the groundwork for a long-term recovery program including:
Green jobs and clean energy;
Restore worker justice, including EFCA and other reforms;
Health care for all;
Retirement security;
Re-regulate the finance system and make the speculators pay to clean-up their mess
Fair Trade and Migration policies


text:
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text:
Fire station
Friday afternoon October 5th 2012 at 3 p.m. the new housing for the Fire Bridage Ommen-Hardenberg, Ommen-Dalfsen and Ommen Police External services Hardenberg was officially opened. With the press of a button 3 silver coloured cloths fell down from which three different vehicles were exposed. This was the official signal for 'Drie Onder Eén Kap' (DOEK) to be declared open.
Prior to that people glanced back to the building process by bct architects and a moment was taken to ascertain the aspects of the sustainable building. The mayor of Ommen, mr. Ahne, proudly presented the new building to all attendees
steni Colour T7 was integrated as facade material during the design phase. As such we were proud to deliver:
• 240 m² RAL2001 in Mat • 250 m² RAL2001 in high-gloss • 300 m² in RAL3013 Mat • 150 m² RAL3013 in high-gloss • 60 m² RAL3011 in Mat • 120 m² RAL3011 in high-gloss • 90 m² RAL 3005 in Mat • 50 m² RAL 3005 in high-gloss • 250 m² SN8671 Halfmat • 50 m² SN8010 Halfmat
In addition to the tremendous flexibility in colours, RAL colors are colors project, the combination of the two gloss also defines this façade. A absolute beauty of a reference project for us. We were granted the following quote by mr. W. Kemperman of BCT architects:
"For the appearance of the building is deliberately optioned for a closed building block with on the infield much space for storage and parking out of sight of the environment. The building has two faces: a rough stone facade that suits the environment in an appropriate manner all around, while the infield a contemporary translation of colors from nature, who from distance becomes visible thanks to the wonderful height accent. The different shades of red, with alternating matte and glossy plates, enclose the courtyard as a warm blanket and give workers a pleasant working environment."
The photos were taken by Laurens Kuipers


text:
Eclipse IDE
1743 Solutions and counting.
The Crank Storyboard Suite enables the rapid design, development and deployment of embedded user interfaces. It provides an Eclipse based visual screen design tool that uses images as control elem...
Lua Development Tools (LDT) is about providing Lua developers with an IDE providing the user experience developers expect from any other tool dedicated to a static programming language.
Like man...
LuaEclipse is a collection of plugins developed for the Eclipse platform that together make an IDE for the development of applications in the Lua programming language. In this environment it's...
Programming Languages
Last Updated on Monday, February 4, 2019 - 13:55 by Simon Bernard
Build and Deploy, Systems Development, Tools, Graphics, UI
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Last Updated on Monday, June 24, 2013 - 16:16 by Luís Jason antos


text:
27 Jun, 2019
Audrey lives on the Central Coast of NSW and is a spritely, active 86 year old. She lost her husband 20 years ago and although she is surrounded by her children and grandchildren, she's fiercely independent. She lives comfortably on the Age Pension and intends to stay in her home 'for the rest of her days' – except a change to her mortgage from interest only to principle and interest nearly derailed her retirement.
Watch Fiona share Audrey's story.


text:
Introduction: Rustic Wooden Towel Rack
This instructable will show you how to make a towel rack from scrap wood and a broom stick.
We recently added wooden frames around the doors and windows in our beach house, made from a Brazilian hard wood called Angelim Pedra. We had lots of this beautiful hard wood left over from this project, and I decided to use it to make some towel racks. I hate to see good wood go to waste!;-)
Here's how I did it...
• scrap wood
• broomstick
• wood glue
• varnish/paint
• nails
• screws
• plastic screw anchors
• Hammer
• Hack saw
• Grinder (optional)
• Paint brush
• Drill
• 13/16 wood bit
• rasp (or file)
• Screw driver
Step 1: Select and Measure Wood
As I mentioned in the introduction, I had a lot of Angelim Pedra to work with. Ironically I only had two broom sticks to work with. Since I wanted to make four of these towel racks, and the broomsticks measured about 100 cm each, I decided to make my racks about 50 cm wide.
The first picture above isn't the exact piece of wood I used, but from the same wood pile.
(This is a piece I used for my Steampunk Socket/Switchplates;-)
Step 2: Grind (or Sand) Wood
This wood is particularly hard, and as I learned in making my steampunk switchplates, it lends itself nicely to being finished with a grinder. The grinder adds a nice rustic effect, by leaving burn marks on the wood. If you don't have a grinder, or the wood you use doesn't lend itslef to this process, you may decide to just sand your wood.
This is also a good time to drill two holes through the base, equidistant from each end, where we will later mount the rack to the wall.
Step 3: Cut End Pieces
To create the ends of the towel rack, which hold the broom stick in place, measure how far out you want the towel to hang, keeping in mind that that you'll lose a bit of space when you attach these pieces to the back.
After you've cut two rectangular pieces (about 4 to 5 inches long) make diagonal cuts on the top of the ends that will face out, and then round the edges a bit with a sander, rasp or file.
(The picture above is of an end piece that I cut too short, but you can see the rounded edge).
Step 4: Prepare End Pieces
This is by far the most complicated and labor intensive step in this process.
I thought about different ways to assemble this rack, and realized I needed to mount it to the wall before I added the broom stick. (Otherwise the stick would interfere with screwing into the wall). To accomplish this, I decided to prepare the end pieces in such a way that I could slide the stick in after the rack was mounted. (Why does that sound so dirty?;-)
So what I did was use a 13/16 wood bit to create the hole. I drilled just far enough to create a small hole through the other side, to eventually put the screws which will hold the stick in place.
Then I used a hack saw blade to make slits from the drilled hole, to the bottom of these pieces, making sure that the space was slightly wider than the holes.
Once these guidelines were cut into the wood, I placed the piece in a clamp, placing the clamp upside down. The bottom part of the clamp I used fit perfectly into the drilled hole, and held the piece in place, while I chiseled out a track to slide the broom stick into.
After chiseling, I used a rasp to smooth out the slots.
Step 5: Assemble
With the end pieces ready, it's now time to nail them to the base. Start hammering the nails in place before assembling. Once the nails are in the end piece, place some wood glue along the edge of the base and then nail on the end piece.
As the wood I was working with is particularly hard, I didn't have to worry about it splitting, but you may have to find another way to attach the ends (maybe elbow brackets or hinges?) if you are using a more delicate wood.
Step 6: Paint and Cut Broom Stick
I forgot to take a picture of the broom stick before I painted it, but it looked just like a broom stick!;-)
I used a coffee colored (if you take your coffee with milk) enamel paint, which in Portuguese is called "Tabac," I guess because it has the appearance of dried tobacco. Select a paint color depending on your desired finished product.
Once the rack is assembled, lay the broom stick in place, and measure to make sure you have a tight fit, inside the end pieces.
Step 7: Varnish (or Stain)
I used a Brazilian varnish called Sparlack Neutrex, the same varnish I used for my Instructable, Painting metal to look like wood.
Step 8: Mount Rack to Wall
If you have tiled walls, you probably want to drill a hole first, and install plastic screw anchors.
Measure the distance between the holes by placing the rack against the wall, and making marks (maybe with a nail or screw) through the holes in the wooden base. Once you have the plastic anchors in the wall, you're ready to mount the rack.
Step 9: Finished Product
Unfortunately I forgot to take pictures of the last step, which is screwing the broom stick into place. But if you've made it this far, hopefully you can figure out the rest on your own!;-)
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7 Discussions
Great job... well done, I solved the fixing problem by drilling the mounting holes 1/2" above the center of the rail.
I thought about that, and it certainly would have been easier, but I decided to go with this more complicated method, so that the mounting screws would be less visible. I'd like to see a picture of yours, if you could post one here in the comments!
This is one I made last year... You can just make out the positions of the mounting holes. I made one recently, also from Oak but more rustic looking like yours, I'm doing an instructable on it at the moment but can't get to my shed much to finish it.
Great idea
Cheers Aeon Junophor
Thanks Junophor! As you can see, I was able to sneak a few steampunk elements into our beach house;-)
Well how am I going to finish this without the last step? lol
Grasshopper - Your comment made me literally "laugh-out-loud!";-)


text:
Charge the killer with a double murder or not?
Suppose she doesn't die but the baby does?
Views: 1516
I have far too much to do, than sit around and compare scars with you - like I said, if you ever have children, come back, we can talk.
That's true, Gregg, but that doesn't mean I have to listen. I have a history with this guy, of which I realize you're not aware.
While I was, as I said, addressing Feenstra, I was indeed attributing a motivation to you, which I later addressed:
"As for Dave, I believe his own comment,: 'You consider a embryo to be a person with full human rights from the moment it is conceived, while in my case, I don't consider it to be a person until it has a functioning brain/neural activity' certainly sounds like someone setting the groundwork for a defense of Roe v Wade. Perhaps he didn't use the actual title, but the implication was certainly there."
If a criminal attacks a woman they are trying to kill HER. that is attempted murder.
False. This why we have crimes like assault, for cases when someone attacks someone else and are not trying to (or likely to) kill them.
If a criminal attacks a woman, for example punching her in the stomach in order to steal her purse, that would be assault. The criminal was not trying to kill her and so an attempted murder charge would be unjust. If the woman was pregnant and miscarried as a result of the punch, whether the miscarriage is considered a homicide or not, the punch is still not an attempted murder.
Let's say for the sake of argument that causing the miscarriage in the above case is considered involuntary manslaughter. The criminal should then be charged with assault (and maybe battery, depending on the jurisdiction) and involuntary manslaughter. Not attempted murder and involuntary manslaughter.
Charge people with the crimes they have committed, absolutely. But charge them with the crimes that they have actually committed. If the sentences for some crimes are inadequate to deter future offenses, etc, then the solution is to fix the sentencing, not to charge them with crimes they have not committed so they'll get a longer/tougher/different sentence.
@Belle Rose;
"Gregg: you take me for a little girl who hasn't seen the world don't you?"
Negative Sweet Rose, I take you only as a post in cyberspace nothing more, nothing less. I make the assumption that there are persons behind the posts I see, but I do not assume to know those persons.
I find it interesting how you can see 'justice served' by a rotating door prison system for those who murder others. And yet somehow view my personal vendetta system having lower value to 'justice served'.
Under the system you support society must put up with a serial killer and an unknown number of murders, a loss for society. Under my system after one murder society suffers no more murders from that individual, a win for society.
Do you not see how your zeal to temporarily punish a murderer with a few years of prison life creates more suffer, pain and death for the innocent who this ex-con will come into contact with after his release in 3.5 years?
@ The Rose;
"I don't wish sexual assault on anyone."
I didn't think you were.
You're making a point I've tried to make many times, Kris. To say that a fetus isn't a person is a legal fact, not a fact like "all metals expand when heated." It's a mere stipulation which can just as easily be undone by the repeal of some law.
On the other hand, how we use language IS a fact, and we do (the majority of us) refer to fetuses as babies or unborn children. This isn't a stipulation.
Not only is referring to the unborn as children common usage, but it reflects the way we most of us think about the unborn. I'd wager even most people who claim that the unborn aren't persons think about their own unborn as persons, in a real sense if not a legal sense.
It always seems so stilted to hear people make the case that the unborn aren't people or aren't human beings. But apparently it doesn't seem stilted to you.
I'm sure she thought of you and referred to you as her baby and her child even before you were born!
And to be clear, I'm pro-choice. I'm just not buying this whole "It isn't a human being until it's born" routine.


text:
Who doesn't like a good shiplap wall?
It seems as if shiplap has been all the rage the last couple years…especially since Joanna Gaines from the hit show FIXER UPPER made it popular.
I liked the look of it and wanted in my house, but without the work or cost of actually putting up shiplap. SO…I painted stripes on my wall to give my house the same effect!
Here is the before and after…
In order to give my wall the shiplap effect, I had to paint on lines on the wall. I used a straight edge ruler, a pencil, some painters tape and black craft paint.
The best part about doing these lines, is that I wanted them to have that rough wood appearance, so I wasn't even that careful about making the lines perfect.
In the end I was very happy with the results!


text:
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text:
Unit 1 of UAE's Barakah nuclear plant to begin ops by early-2020
Nawah Energy Company, the plant's operator, said it has "entered the next phase of preparations for nuclear operations"
Unit 1 of the UAE's Barakah Nuclear Energy Plant will commence operations by 2020.
Nawah Energy Company, the plant's operator, has entered the next phase of preparations for nuclear operations.
The company recently completed a comprehensive operational readiness review to generate a schedule for the start-up of the plant.
Nawah's review forecasts that "the loading of nuclear fuel assemblies" required to commence nuclear operations at Unit 1 "will occur between the end of 2019 and early 2020".
Construction work on Unit 1 was reported to have been completed this March.
The projection for Unit 1's start-up reflects the time required for Barakah's nuclear operators to complete operational readiness activities and to obtain necessary regulatory approvals.
Operators will begin loading nuclear fuel assemblies and initiate operations after all preparations for nuclear operations are completed, and Nawah has received an operating license for Unit 1 from the Federal Authority for Nuclear Regulation (FANR).
Commenting on the development, Mark Reddemann, chief executive officer of Nawah, said: "Our review relied on global nuclear standards to analyse the remaining work required for nuclear operations, and we are confident that this new projection for fuel loading gives Nawah a nuclear-centric and conservative schedule to deliver nuclear operations in alignment with the highest standards of safety and quality.
"By achieving high standards in Unit 1, Nawah is setting a robust precedent for the subsequent operation of Units 2, 3, and 4, as well as for the regional industry."
Emirates Nuclear Energy Corporation (ENEC), on behalf of Nawah, submitted the Operating License Application for Units 1 and 2 of the plant in 2015.


text: Nucleotides: The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Polymorphism, Single Nucleotide: A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.Adenine NucleotidesGuanine NucleotidesAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Purine Nucleotides: Purines attached to a RIBOSE and a phosphate that can polymerize to form DNA and RNA.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Nucleotides, CyclicGuanine Nucleotide Exchange Factors: Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Pyrimidine Nucleotides: Pyrimidines with a RIBOSE and phosphate attached that can polymerize to form DNA and RNA.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Genes, Bacterial: The functional hereditary units of BACTERIA.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Kinetics: The rate dynamics in chemical or physical systems.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Genetic Variation: Genotypic differences observed among individuals in a population.Codon: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Guanosine Triphosphate: Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Guanosine Diphosphate: A guanine nucleotide containing two phosphate groups esterified to the sugar moiety.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Thymine Nucleotides: Phosphate esters of THYMIDINE in N-glycosidic linkage with ribose or deoxyribose, as occurs in nucleic acids. (From Dorland, 28th ed, p1154)Cytosine NucleotidesNucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Genes, Viral: The functional hereditary units of VIRUSES.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Bacterial Proteins: Proteins found in any species of bacterium.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.Haplotypes: The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.Deoxyadenine Nucleotides: Adenine nucleotides which contain deoxyribose as the sugar moiety.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Uridine Triphosphate: Uridine 5'-(tetrahydrogen triphosphate). A uracil nucleotide containing three phosphate groups esterified to the sugar moiety.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.DNA Repair: The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Base Composition: The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.Introns: Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.Deoxyguanine Nucleotides: Guanine nucleotides which contain deoxyribose as the sugar moiety.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Adenosine Monophosphate: Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Deoxycytosine Nucleotides: Cytosine nucleotides which contain deoxyribose as the sugar moiety.Inosine NucleotidesOligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)RNA, Ribosomal: The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)Ribonucleotides: Nucleotides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed)Genetic Predisposition to Disease: A latent susceptibility to disease at the genetic level, which may be activated under certain conditions.2',3'-Cyclic-Nucleotide Phosphodiesterases: Nucleoside-2',3'-cyclic phosphate nucleotidohydrolase. Enzymes that catalyze the hydrolysis of the 2'- or 3'- phosphate bonds of 2',3'-cyclic nucleotides. Also hydrolyzes nucleoside monophosphates. Includes EC 18.104.22.168 and EC 220.127.116.11. EC 3.1.4.-.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Gene Frequency: The proportion of one particular in the total of all ALLELES for one genetic locus in a breeding POPULATION.Rho Guanine Nucleotide Exchange Factors: Signaling proteins which function as master molecular switches by activating Rho GTPases through conversion of guanine nucleotides. Rho GTPases in turn control many aspects of cell behavior through the regulation of multiple downstream signal transduction pathways.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Viral Proteins: Proteins found in any species of virus.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Oligoribonucleotides: A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Molecular Weight: The sum of the weight of all the atoms in a molecule.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Nucleotide Transport Proteins: Proteins involved in the transport of NUCLEOTIDES across cellular membranes.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Linkage Disequilibrium: Nonrandom association of linked genes. This is the tendency of the alleles of two separate but already linked loci to be found together more frequently than would be expected by chance alone.Phosphoric Diester Hydrolases: A class of enzymes that catalyze the hydrolysis of one of the two ester bonds in a phosphodiester compound. EC 3.1.4.Adenine Nucleotide Translocator 1: A subtype of mitochondrial ADP, ATP translocase found primarily in heart muscle (MYOCARDIUM) and skeletal muscle (MUSCLE, SKELETAL).Nucleotide Mapping: Two-dimensional separation and analysis of nucleotides.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Deoxyuracil Nucleotides: Uracil nucleotides which contain deoxyribose as the sugar moiety.Mitochondrial ADP, ATP Translocases: A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.Templates, Genetic: Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.RNA, Transfer: The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.Guanosine Monophosphate: A guanine nucleotide containing one phosphate group esterified to the sugar moiety and found widely in nature.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Plant Viruses: Viruses parasitic on plants higher than bacteria.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Endonucleases: Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Base Pairing: Pairing of purine and pyrimidine bases by HYDROGEN BONDING in double-stranded DNA or RNA.Cyclic Nucleotide Phosphodiesterases, Type 1: A CALCIUM and CALMODULIN-dependent cyclic nucleotide phosphodiesterase subfamily. The three members of this family are referred to as type 1A, type 1B, and type 1C and are each product of a distinct gene. In addition, multiple enzyme variants of each subtype can be produced due to multiple alternative mRNA splicing. Although the type 1 enzymes are classified as 3',5'-cyclic-AMP phosphodiesterases (EC 18.104.22.168), some members of this class have additional specificity for CYCLIC GMP.Adenylyl Imidodiphosphate: 5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.Adenine: A purine base and a fundamental unit of ADENINE NUCLEOTIDES.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.DNA Replication: The process by which a DNA molecule is duplicated.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.Sequence Deletion: Deletion of sequences of nucleic acids from the genetic material of an individual.DNA-Directed DNA Polymerase: DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.RNA Splicing: The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.ras Guanine Nucleotide Exchange Factors: A family of GUANINE NUCLEOTIDE EXCHANGE FACTORS that are specific for RAS PROTEINS.Receptors, Purinergic P2: A class of cell surface receptors for PURINES that prefer ATP or ADP over ADENOSINE. P2 purinergic receptors are widespread in the periphery and in the central and peripheral nervous system.Ribonuclease T1: An enzyme catalyzing the endonucleolytic cleavage of RNA at the 3'-position of a guanylate residue. EC 126.96.36.199.Deoxyribonucleotides: A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Ultraviolet Rays: That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.Cyclic GMP: Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)Single-Strand Specific DNA and RNA Endonucleases: Enzymes that catalyze the endonucleolytic cleavage of single-stranded regions of DNA or RNA molecules while leaving the double-stranded regions intact. They are particularly useful in the laboratory for producing "blunt-ended" DNA molecules from DNA with single-stranded ends and for sensitive GENETIC TECHNIQUES such as NUCLEASE PROTECTION ASSAYS that involve the detection of single-stranded DNA and RNA.NAD: A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)Receptors, Purinergic P2Y2: A subclass of purinergic P2Y receptors that have a preference for ATP and UTP. The activated P2Y2 receptor acts through a G-PROTEIN-coupled PHOSPHATIDYLINOSITOL and intracellular CALCIUM SIGNALING pathway.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Ribonucleases: Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.GuanineMultigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)3',5'-Cyclic-AMP Phosphodiesterases: Enzymes that catalyze the hydrolysis of CYCLIC AMP to form adenosine 5'-phosphate. The enzymes are widely distributed in animal tissue and control the level of intracellular cyclic AMP. Many specific enzymes classified under this heading demonstrate additional spcificity for 3',5'-cyclic IMP and CYCLIC GMP.Sequence Homology: The degree of similarity between sequences. Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Thionucleotides: Nucleotides in which the base moiety is substituted with one or more sulfur atoms.Poly A: A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Amino Acid Substitution: The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.5' Untranslated Regions: The sequence at the 5' end of the messenger RNA that does not code for product. This sequence contains the ribosome binding site and other transcription and translation regulating sequences.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Adenine Nucleotide Translocator 2: A subtype of mitochondrial ADP, ATP translocase found primarily in FIBROBLASTS.Pyrophosphatases: A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.Consensus Sequence: A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.Nucleosides: Purine or pyrimidine bases attached to a ribose or deoxyribose. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Xeroderma Pigmentosum Group A Protein: A ZINC FINGER MOTIF protein that recognizes and interacts with damaged DNA. It is a DNA-binding protein that plays an essential role in NUCLEOTIDE EXCISION REPAIR. Mutations in this protein are associated with the most severe form of XERODERMA PIGMENTOSUM.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Purines: A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Guanylyl Imidodiphosphate: A non-hydrolyzable analog of GTP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It binds tightly to G-protein in the presence of Mg2+. The nucleotide is a potent stimulator of ADENYLYL CYCLASES.Genes, Regulator: Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Genetic Code: The meaning ascribed to the BASE SEQUENCE with respect to how it is translated into AMINO ACID SEQUENCE. The start, stop, and order of amino acids of a protein is specified by consecutive triplets of nucleotides called codons (CODON).Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).Sequence Analysis: A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Uridine Diphosphate: A uracil nucleotide containing a pyrophosphate group esterified to C5 of the sugar moiety.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Guanosine: A purine nucleoside that has guanine linked by its N9 nitrogen to the C1 carbon of ribose. It is a component of ribonucleic acid and its nucleotides play important roles in metabolism. (From Dorland, 28th ed)Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.DNA, Single-Stranded: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.Genes, Fungal: The functional hereditary units of FUNGI.Base Pair Mismatch: The presence of an uncomplimentary base in double-stranded DNA caused by spontaneous deamination of cytosine or adenine, mismatching during homologous recombination, or errors in DNA replication. Multiple, sequential base pair mismatches lead to formation of heteroduplex DNA; (NUCLEIC ACID HETERODUPLEXES).Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.3' Untranslated Regions: The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences.Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Genome-Wide Association Study: An analysis comparing the allele frequencies of all available (or a whole GENOME representative set of) polymorphic markers in unrelated patients with a specific symptom or disease condition, and those of healthy controls to identify markers associated with a specific disease or condition.Nucleotidyltransferases: A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.Inosine Monophosphate: Inosine 5'-Monophosphate. A purine nucleotide which has hypoxanthine as the base and one phosphate group esterified to the sugar moiety.DNA Adducts: The products of chemical reactions that result in the addition of extraneous chemical groups to DNA.Nucleotide Deaminases: Catalyze the hydrolysis of nucleotides with the elimination of ammonia.Mosaic Viruses: Viruses which produce a mottled appearance of the leaves of plants.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Genetic Association Studies: The analysis of a sequence such as a region of a chromosome, a haplotype, a gene, or an allele for its involvement in controlling the phenotype of a specific trait, metabolic pathway, or disease.Phosphates: Inorganic salts of phosphoric acid.


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Rumors about the future of Assassin's Creed never exactly cease, but they've certainly picked up steam recently. Much of the hoopla concerns an intriguing Easter egg featured in The Division 2. Assassin's Creed Wiki Community user, AlifMorrisonudin, found the Easter egg, which consists of a "Valhalla" poster. It's a standard piece of art, really, featuring a Norse ruler of some sort carrying a staff and spherical artifact. However, upon closer inspection, the spherical artifact appears to be an Apple of Eden from Assassin's Creed lore.
See a screenshot of the "Valhalla" poster shared by AlifMorrisonudin below:
YouTuber JorRaptor took this information and dug a little deeper. Interestingly, this is not the first time talk of a Viking setting has circulated about the Assassin's Creed franchise's future. Art from former Ubisoft artist Michel Nucera made the rounds in 2018. Nucera's art, which has since been deleted from his ArtStation profile, depicted a character in Assassins-esque garb aboard a ship evidently inspired by those from the Viking period. Nucera explained that this particular piece of concept art, referred to as "Assassin's Creed Ragnarok," was merely a passion project, with no connection whatsoever to Ubisoft's plans for the long-running series.
Check out JorRaptor's analysis of the rumor in the video below. The YouTuber makes mention of Nucera's concept art at around the 3:15 timestamp:
Of course, none of the details above explicitly point to the Viking rumors being based in fact. Plus, as previously mentioned, this is not the only rumor to make bold suggestions about the next Assassin's Creed entry. For instance, a recent bit of speculation purports the series may finally return to Rome. Akin to talk of the Vikings era, there exists no concrete evidence to corroborate such claims. Still, it would be fun to see how Ubisoft's flagship franchise might explore Norse mythology, especially on the heels of Egyptian and Greek lore appearing in Origins and Odyssey, respectively.
Easter Egg in The Division 2 Fuels Assassins Creed Vikings Rumor


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Working wildfires over the North American summer corrals pilots and engineers into small-town bars nightly to consume a bucket of wings on beer-soaked bench tops. It's the most wonderful time of the year (a working pilot has less time to complain…) and with the coagulation of talented minds, chicken parts (where do they get that many little legs & wings?) and fluids in a confined operating space, you soon have some pressing world issues sorted.
Nothing useful like how to end world poverty or what to do now there are no more plastic bags at the supermarket but instead the toughest questions of our business; "Where's the smart money in the utility helicopter game right now?"
It turns out the answer is as complicated as the different roles our helicopters occupy and depends on where we are in the cycle. If I open my logbook and review the halcyon summers of 2005 to 2007 I see exploration and drill moving dominating history. Fires were something we did in the few spare days between contracts. Then the burning ambition was a seat in a Bell 204's or 205's and Bell 212's started to emerge as the OEG sector demanded composite fuselages, magenta lines and Italian designed leather for their rig workers. Fleets of Bell 212's were repurposed as utility operators worldwide shrugged off despair over the fuel burn and reduction in useful load (versus a Bell 205) to embrace twin-engine redundancy. As the decade evolved, Honeywell helped to solidify the relevance of the Bell 212 pushing the Bell 205's T53 Engine overhaul price past seven figures. In 2012 you could buy a Bell 212 for the price of the T53 engine and that reminded operators that it was a sensible place to spend a day bucketing fires. Forestry paid the fuel bill anyway.
Come 2008, the Global Financial Crisis strangled the industry in a rear naked choke and by 2012 many pilots had tapped out to become plumbers, engineers found project work and companies treaded water waiting for the future. The future arrived in the form of UAV's and drones which killed off the photography work as times of peace crushed gold prices and the need to explore. Cleaner means of combustion made Oil and Gas (OEG) unpopular and very quickly the business of "chasing fires" shifted from low priority (unless it was your town burning in 2006) to become the big idea for summer.
Until 2016, the business of chasing fires was still unpredictable but the world changed after the Fort McMurray Fire made world headlines and the 2017 Elephant Hill Fire blocked out the sun to exacerbate Antarctic melting in spectacular fashion. According to Fire Ecologists the behaviour since 2016 was not predicted until 2050 (who would even admit to that mistake) but since then a burnt 24,000 square kilometres of British Columbia alone, has cost the local government more than $1000,000,000 (that's a BILLION for you engineers out there) and proved you can't fight fires with UAV's yet. Every helicopter operator from Portugal to Sydney is hoping that 2019 will be a trifecta year.
With Fires the focus, the contract your bank manager really wants you to have is an Initial Attack [IA] Contract. It comes with not so much flying but copious amounts of stability and regular income and it was during the bidding of IA contracts in the mid-2000's that the Bell 412 first shook up the status quo. Bell 412's seductively won a host of contracts in the provinces of Ontario and British Columbia. Until then the only 412's seen in Canada were camouflage green and hover taxied everywhere but since have proven to be better than good and a model that has been replicated across Canada. Those four blades, two engines, and single pilot are an absolute game changer.
So why?
An Initial Attack configuration is one helicopter with a means of collecting and dropping water plus a 4-5 person firefighter team equipped with days of food, shelter, tools, hose, and pumps. The mission is reactive - prevent little problems becoming big problems. In the firefighting world, few of the tools have changed since Moses had a conversation with the burning bush.
VIEW OUR BELL 412 SP - 3,699 Hours | Recent 5 Yr/5000 Hr Inspection | U.S.A
You need copious rain or a mix of shovels, picks, water, and muscle. The sooner you can deliver those resources, the more likely you will prevent escalation.
The Bell 412 with the high cruise speed (thanks to the composite Main Rotor and fancy Blades) delivers that response over 25% faster than the 205/212. Once on scene, the compact BH412 Main Rotor offers better options for confined landings and when freed from the transportation role, a tanked aircraft quickly moves into suppression work delivering 304 US Gallons a shot. A significant feature of a tanked aircraft is you don't need a highly qualified vertical reference pilot with 1000's of hours on a bucket and 100ft line. You don't even need to land to configure to longline. Just punch off the snorkel, start sucking and off to work. Anyone who can hover and manage a couple of tasks concurrently can be quickly trained on the tanked Bell 412.
As you now realize I know the Bell 412 market intimately from the Classic to the EP, so if you are interested to continue the conversation I encourage you to register your interest. There is plenty more to share and I'll support your enquiry with expectations around W&B, aircraft configuration, airspeed and weight limits, operational considerations with tanked helicopters and our best numbers around operating costs.
Call or write today.


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6 Word story Start
This project starts with us examining the most famous 6 word story.
"For Sale; Baby Shoes. never worn" by Earnest Hemingway.
The typical interpretations of this story is the grief of a mother who has lost her baby who is giving the shoes away to benefit another child. I am an optimist, everything I examine I put a happy idea with it. I saw this as someone starting a new adventure, may it be a job, life, education, or relationship. Approaching it slowly with baby steps. Huffington Post did an article in 2014 when Tumblr had this topic trending and had several stories featured. Among my favorites in the article is a user written story "I asked. You answered with silence." and I find this such a strong story because silence is one of the most powerful things a person a can (not) say.
Don Goble
10/3/2016 06:39:35 am
This is a terrific blog post Courvaun! Great insight and outlook on Hemingway and awesome example choice. Silence can say so much.
Leave a Reply.
16 year old diva
April 2019


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Recently found by viaLibri....
2019-11-08 22:42:10
The Photobooks of Nobuyoshi Araki: The Kris Sperry Collection
Various: Various, 1964-2009. We are pleased to announce a new catalog available from Underground Books featuring a significant collection of the photobooks of the controversial Japanese photographer Nobuyoshi Araki. The catalog features 227 entries totaling nearly 300 items, with a substantial number of them being limited editions, signed, inscribed, or with hand drawn caricatures (often comically pornographic, in Araki's signature style). At present, the collection is only available as a complete lot, offered at $35,000. Due to the explicit nature of the content, the catalog is not linked on our website. We have print or PDF versions of the catalog available upon request. Please message or e-mail us to request a copy of the catalog. Item #5283. [Attributes: First Edition; Signed Copy]
Vendeur: UndergroundBks (underground_books_llc) [Carrollton, Georgia, US]
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Browse more rare books from the year 2009
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College Shabbatons
What Is This?
Wanna get away somewhere? Bored of the Jewish life at your college? Want to share your love for Jews and Judaism with other Jews?
Try running a Shabbaton somewhere which would appreciate the influx of enthusiastic young blood! And since you're a college students, why not go to another college!
Here's why it works: You are guitar players, philosophers, dancers, basketball players, pre-med students, econ majors, beer drinkers, 'The Office'-watchers, singers, and Facebook users. You are smart, funny, friendly, energetic, well-educated and cultured, embarking on the lofty and admirable journey of a college education. And you are knowledgeable, enthusiastic, welcoming, and passionate about your Judaism. Who could say 'no' to a group like that?
Each college's situation and populace is different, and thus has different needs and expected goals which you'll have to think about. But here are some general ones you might want to keep in mind when planning your excursion:
- Meet Jewish students who are different then yourself, giving all the opportunity to befriend and learn from each other.
- Reach fellow students in a non-intimidating, non-denominational, and not-institutionalized manner that is uniquely Jewish.
- Engage students in meaningful, content-filled, and inspiring Jewish experiences in environments often lacking just that.
- Impart Jewish education, religious acclimation, and cultural enrichment through shared Jewish experiences.
- Provide multiple avenues for students to connect to Judaism - be it through Jewish community, Jewish culture and rituals, Jewish knowledge or Israel - and all through peer-to-peer relationships. Inform them about Birthright, MASA, Hillel, Chabad and the plethora of Jewish organizations, resources, and activities which are available to them.
- Inspire Jewish students to get involved in Judaism, whether on their own, with their community, or through one of the aforementioned programs.
- Engender a sense of Jewish community on campus through lively and participatory programming.
- Develop and foster relationships and connections between Jewish students on colleges campuses across America.
See what's out there
- Small and Mighty - First find out what this is here, then look /search through the list here or here
- Hillel's college search - Here you can search for all colleges in specific regions or near an address. This site has a basic overview of Jewish life on campus - with undergrad and grad population, services offered, and staff contact info. If there's some sort of institution there, try and contact whoever is in charge - they'll probably have a lot of information and might offer to help you.
- Chabad's college search - See if there's a Chabad house nearby and if so, get in touch with the Chabad rabbi - ditto on the info and help.
- College Prowler has great overviews and insights into general life on campus.
- www.bestplaces.net - While not for a college itself, you can enter the city/state/zip and find out the general overview, as well as a rough religious breakdown of a specific location
- Be careful not to step on anyone else's toes - Chabad or Hillel might be worried that you're trying to 'steal' their customers. If that becomes a concern, make it clear that you have no intention of doing so, and merely want to help bring the students a meaningful Jewish experience. Maybe even offer to work with them, assuming the positives (contacts, resources, facilities) would outweigh the negatives (social detriments, institutional turn-offs, religious demands).
Ways to find people:
- Facebook: search in the college's network (or for the name of the college and) for the words "Jewish", "Jews", "Shabbat", "Israel", "Shalom", "Hillel", etc. - trust me, you never know what will turn up.
- MySpace: http://browseusers.myspace.com/Browse/Browse.aspx - go to the 'Advanced Search' tab and search with X miles of a specific zip code for religion=Jewish, age=18-25?
- Network: ask people from school/camp/home if they know Jews at that college or in that city. It's a small, small, small, small Jewish world.
- Contact the local Jewish Federation/Jewish community - ask someone there if they know of college students nearby.
- Speak to Hart (917 992 8090) - he has contacts at a lot of schools across America.
Once you find local students, get in touch with them - send them an email, give them a call. Tell them what you're doing, why you're coming, how excited you are, and see if they're interested. Ask for help and try to get them involved; having a point person on the ground will makes things easier for you, and it's also a great way to get them active and committed.
Most importantly, find a good week for you and your fellow adventurers. If you can find a weekend which is during or nearby to a Jewish holiday - even better, as holidays bring out the best in unconnected Jews! (For example, if it's before Pesach you can go for a chametz eating contest, or before Rosh Hashana parade around with shofars.) You also want to keep in mind the possibility for a follow-up Shabbaton, so the last weeks of schools aren't ideal.
Programs to Run
The challenge is running a Shabbaton which is both fun, inviting, and attractive, and containing meaningful and interesting Jewish content. A formidable challenge, but one for which many years of Jewish upbringing and education should have prepared you better than anyone else.
Don't underestimate the value of Jews just hanging out, playing Jewish geography, hearing each others life stories - that's some of the most meaningful stuff out there. In the end of the day, simple heart to heart conversations is really what its all about. And if you're a person filled with Jewish content, you'll bring the Jewish content without even trying.
Of course, you'll have to see what the place needs and wants but here are some possible ideas:
General Ideas:
- Discussion on Jewish philosophy over some good old beer drinking
- BBQ (having a bonfire is much better, and more manly, than using a grill)
- Break out a guitar, drum, flute (chalil) or your voices, and have a jam session
- Coupling the Shabbaton with a Motzei Shabbat game of capture-the-flag-with-dart-guns. Or similar super awesome but not necessarily Jewish things that will get people talking and wanting to join just because these Shabbatons are super creative, cool, and fun [thanks Tal!]
One of the best things about Shabbat is that it is a built-in complete package of Jewish content, community, culture, and meaning. Unlike a random night, when you'll have to plan a whole program or activity, going somewhere for Shabbat already provides you with most of that.
Schools with Contacts
List of schools (Why not join the "Heart to Heart" GoogleGroup while you're at it and stay in the loop with "Heart to Heart"'s activities)


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A couple days ago, I shared with you all that I was struggling to decide on a destination–the struggle continues. Last time, I considered Iceland (which is still very much on the list). Today, let's consider a vastly different but equally awesome possibility: Costa Rica.
(Casey Mahaney, Lonely Planet)
(Corey Wise, Lonely Planet)
(Lucas J. Gilman/Aurora Photos)
(Lisa Whitwam/Getty Images)
(Donna and Steve Omeara/Photo Library)
(Roy Toft)
(Christian Aslund for Lonely Planet)
Things I'd like to do in Costa Rica:
-Scuba Dive
-Visit the Serpentarium
-See a sloth & other critters
-Eat local food, especially the fruit
-Swim under a waterfall
Where would you most like to travel to? Where is your favorite place you've ever been?


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Homebrew Spotlight: Lepi
Lepi Medium Humanoid (lepi, animalfolk) (12 RP) Racial Traits +2 Dexterity, -2 Intelligent or Wisdom (0 RP) Humanoid: Lepi are humanoid with the Lepi subtype. (0 RP) Medium Size: Lepi are medium so have no bonuses or penalties due to their size. (0 RP) Standard Speed: Lepi have a base speed of 30 feet (0 RP) Lowlight Vision: Lepi can see twice as far as … Continue reading Homebrew Spotlight: Lepi
Homebrew Spotlight: Lycan
Lycan Medium monstrous humanoid (shapechanger, lycan, natural) (14 RP) Racial Traits +2 to two physical and -2 to one mental ability score. Lycan are sociable and agile, but often lack common sense. Canines are the most common Lycan, this and alternate choices are in the table below in the clan section; other varieties can be taken with the DMs permission, even water-base creatures. (1 RP) … Continue reading Homebrew Spotlight: Lycan
RPG-a-Day: Favorite RPG I Never Get To Play
My favorite RPG that I never get to play is BESM: I have a copy of Big Ears, Small Mouse that I've played twice. It is a supplement to BESM, but uses animal and insect PCs and NPCs. I was a grasshopper mouse barbarian and my son was a dragonfly rogue, my husband ran it in a Redwall-like world. It was a fun game. He … Continue reading RPG-a-Day: Favorite RPG I Never Get To Play
First Looks Relics a Game of Angels RPG Kickstater
Relics is packed to the gills with depth. There is so much content to draw inspiration from that it is shocking that Tin Star was able to jam this much information in just 275 pages. At the point where I thought i had have seen all that Relics had to offer me I was further surprised. Continue reading First Looks Relics a Game of Angels RPG Kickstater
RPG-a-Day: Most Intellectual RPG Owned
The Most Intellectual RPG Owned The most intellectual RPG I own is Chivalry and Sorcery, but I haven't played it. Everyone else say that its too complicated, similar to Central Casting . It was mostly bought for the tables, and has great city creation rules although it's very rule-heavy. Related Links: RPGaDay: Favorite Tie-In Novel/Game RPGaDay: Coolest RPG product/book Continue reading RPG-a-Day: Most Intellectual RPG Owned
Dog Might Games: Countdown Action Edition Review
Hands on Review of Dog Might Games New Game Countdown Action Edition Continue reading Dog Might Games: Countdown Action Edition Review
Homebrew Spotlight: The Travelers, Nomadic Halflings
The Travelers: Nomadic Halflings 9/23/2016 Small humanoid (halfling) (14 RP) Racial Traits +2 Dexterity, +2 Constitution or Wisdom, -2 Intelligence. Nomadic Halflings are nimble, hardy and strong-willed. (1 RP) Advanced Dexterity: Nomadic Halflings receive a +2 to Dexterity. They never lose their Dexterity bonus due to age and instead gain 1 point of Dexterity every time they increase in age. This replaces their age related … Continue reading Homebrew Spotlight: The Travelers, Nomadic Halflings


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Pan Progress Report April 2000
Roy and I visited Odyssey Restoration on Tuesday, March 7, where work is nearing completion on the wood body frame. Because of other cars requiring wood work, their wood specialist has been spending somewhat less time on our Pan.
We picked up the second of the two Firestone wheels we purchased and we had Tom Rasmussen examine it and he felt, as we did, that the wood parts of the wheel are restorable. We now have two restorable wheels and, hopefully, enough rims and other metal parts needed to have two more wheels built.
We will soon be deciding on brand, type, and size of tires we want on this car. Our #1 Pan has 34 X 4 1/2 tires while Roy's car has 33 X 4 as the cars were originally equipped. There is a third size available, 35 X 5, which also fits a 25-inch rim. Originally, Pan's came with 33 X 4 tires, the fronts being smooth and the rears were treaded.
Locally Shirley Block has rebuilt the carburetor and has it ready to go. The frame has been sandblasted and is in Little Falls at Ultra Image for power coating.
In the March issue of the Minnesota Street Rod Association newsletter, there was an article by Jim Davis which included several pictures of cars under restoration including our Pan. Jim mentions that one of the highlights of the open house was seeing our Pan and he included a little history of the Pan Motor Company.
Anyone who is looking for a place to invest that income tax refund talk to Duane or me. We know where you can get 8% on your money.
Warner Noble
Pan Progress Report May 2000
This past month has seen little progress on the Pan. Other work at Odyssey Restoration has allowed Chuck, their wood specialist, to take a break from the Pan for a few weeks.
Roy and I went to Odyssey on Monday, April 10, and brought back all the wheels and parts. It appears we have two restorable wheels for the front of the car and will need to have two wheels made for the rear.
The frame should be returning from the powder-coaters shortly. We plan to have the restored chassis and running gear assembled in time to display at our car show.
Anyone with money left over after taxes and interested in investing it at eight percent should contact Duane or myself. The response to date has been very positive, but we have some items of considerable expense upcoming.
Warner Noble
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St Cloud Antique Auto Club, Inc.
All rights reserved.
updated 1/2/2012


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Porsche 911 highlights
Source: Flickr
The 2005 Porsche 911 redesigned rear-wheel-drive models.
The styles are actually offered as coupes and also convertible Cabriolets.
They all possess a rear-mounted, flat resisted 6-cyl motor.
The upgraded Porsche 911 has discreetly changed designing and also measurements
and also transformed the inner parts. There are 2 types of 911: The Carrera, along with a
325-hp 3.6 liter motor and The Carrera S, along with 355-hp 3.8 liter.
The 6-speed manual transmission is standard to all 911s. Optional,
our team could choose the 5-speed automatic with hand-operated shift gateway as well as
steering-wheel commands. Requirement, the 911s possess antilock 4-wheel
disc brakes, antiskid/traction control and also front edge air bags.
The redesigned Careras consist of side air bags in the windowsills for head
security. In the convertibles styles, we discover a power leading as well as heated
glass back home window. A suspension along with reduced flight elevation as well as shock
absorbers are regular on the Carrera S and also extra on the Carrera. This
system aids readjusting firmness within vehicle driver selectable Regular as well as
Sport designs. All other Porsche versions have 18 in wheels, but the Carrera
S has 19s. An alternative for the Carrera is a Sporting Activity Chrono Plan. That consists of
a dashboard-mounted stop watch as well as it enables affecting several engine as well as
framework commands to boost steering performance. All 911s offer
a navigation unit.
Visit Here: https://poptype.co/catherine-kimball/porsche-macan-lease-deals-miami


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Decorating trees during what has become known as the Christmas season began in Germany during the early 1800's. Nuts coated with sugar, apples and other pieces of candied fruit hung among the evergreen branches were the first German Christmas ornaments. Ornaments cut from gingerbread dough and marzipan also became popular. Later eaten by the children, these expensive treats were out of reach for all but the wealthy.
By the mid 1800's the glass-blowers of Lauscha began to produce hand-blown glass ornaments to mimic the fruits and nuts the wealthy were hanging on their trees. Long known for the quality of their glassware (medicine bottles, barometers, marbles and eye glasses) the glass-blowers expanded the ornament business into a cottage industry with the men doing the actual blowing of the ornaments, the women doing the silvering of the insides (early in the industry with either lead or mercury, then later on with a mixture of sugar-water and silver nitrate) and the children painting the outside. Thus began the tradition of the beautiful glass ornaments that Germany became famous for.
Lovely glass spheres aren't the only German Christmas ornaments that valued by collectors. Dresden began producing gaily painted, embossed paper ornaments. Decorations of pressed tin with brightly lithographed pictures were being produced in other parts of Germany and thin strips of metal called "angel hair" began to show up on trees around the country. This "angel hair" is what we now lovingly call "icicles". Ornaments were also made out of wood, walnut shells, pewter and wax.
Queen Victoria's Prince Albert (a native of Germany) introduced the glass ornaments to England and by the 1870's German Christmas ornaments were being exported to Great Britain. Ten years later, F.W. Woolworth (the American Five & Dime giant) discovered the lovely glass ornaments during a trip to Europe and began importing them to the United States.
Though the popularity of the ornaments declined during both WWI and WWII, Germany still imports some ornaments to the United States every year. German Christmas ornaments, especially the older ones are still valued by collectors. The most popular German Christmas ornament shapes are Santa and Mrs. Claus (or St. Nick), Mary and Joseph, rocking horses, soldiers, pinecones and, of course, the German Christmas Pickle.
Of the many contributions that the German culture has made to modern society, perhaps one of the most beautiful and enduring is that of the Christmas tree and decorations.
– Sherry Law


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In Italy, we debate about lobbying's regulation since the birth of Constitution. Nonetheless, our country has not been still provided with a general rule for lobbying, and in so doing it leaves an important normative gap if we consider the importance assumed by the groups of pressure in the political debate. However, useful suggestions came from regional dimension; in fact, the political systems of Toscana, Abruzzo, Molise, Lombardia and Calabria have recently introduced rules that offer an important testing ground to check the effectiveness of the regulatory framework of a so controversial discipline.
Visualizza il documento integrale.


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Technological progress never stops because curious and creative minds are always producing innovation. As the people of the early 21st century, we are experiencing the fourth industrial revolution right now which will result in far-reaching changes in all areas of our lives.
Artificial intelligence, machine learning and IoT are revolutionizing the retail sector and communication with customers. At KPS's stand, discover what role language recognition will play in future – "shopping with Alexa - SAP Hybris and language recognition." Be also part of our presentation "Digital Management - the art of controlled digital change" with Mr. Stefan Metzger (Partner, KPS AG) as Speaker in the Omnichannel Forum on February 27th from 2:30 to 3:00 p.m.
Contact Ms Katri Ojala to arrange an appointment with KPS-Experts at the EuroCIS.
You will find us in Hall 10/B42 at the SAP partner stand. We look forward to engaging in interesting discussions with you.


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Kid Games
por VishwasG
Kid games at http://www.tutitu.tv/games are built on AS3 and PureMVC.
VishwasG Indore, India
Mi perfil
I am a Html5, Unity & Animate CC/Flash developer working with the technology, since 2003. Till now, I have developed hundreds of successful software for entrepreneurs, start-ups, and companies. I proud myself as a passionate and ambitious programmer providing freelancing and contract based services for coding with Html5, Unity (C#), AS3.0, ( actionscript ), CreateJS suite and OpenFl, for both desktop as well as mobiles. I know sufficiently about using Photoshop and Illustrator and have a good designing sense too. Thanks for going through my profile. :)