text: Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Actin Cytoskeleton: Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.Actin Depolymerizing Factors: A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.Actin Capping Proteins: Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cytoskeleton: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Phalloidine: Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Thiazolidines: Reduced (protonated) form of THIAZOLES. They can be oxidized to THIAZOLIDINEDIONES.Gelsolin: A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Cytoskeletal Proteins: Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.Profilins: A family of low molecular weight proteins that bind ACTIN and control actin polymerization. They are found in eukaryotes and are ubiquitously expressed.Cytochalasin D: A fungal metabolite that blocks cytoplasmic cleavage by blocking formation of contractile microfilament structures resulting in multinucleated cell formation, reversible inhibition of cell movement, and the induction of cellular extrusion. Additional reported effects include the inhibition of actin polymerization, DNA synthesis, sperm motility, glucose transport, thyroid secretion, and growth hormone release.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Myosins: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Bicyclo Compounds, Heterocyclic: A class of saturated compounds consisting of two rings only, having two or more atoms in common, containing at least one hetero atom, and that take the name of an open chain hydrocarbon containing the same total number of atoms. (From Riguady et al., Nomenclature of Organic Chemistry, 1979, p31)Actin-Related Protein 2-3 Complex: A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Contractile Proteins: Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Pseudopodia: A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.Tropomyosin: A protein found in the thin filaments of muscle fibers. It inhibits contraction of the muscle unless its position is modified by TROPONIN.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Actin-Related Protein 2: A PROFILIN binding domain protein that is part of the Arp2-3 complex. It is related in sequence and structure to ACTIN and binds ATP.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Protein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.Actin-Related Protein 3: A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. It is expressed at higher levels than ARP2 PROTEIN and does not contain a PROFILIN binding domain.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Recombinant Proteins: Proteins prepared by recombinant DNA technology.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.PhosphoproteinsPolymerization: Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Two-Hybrid System Techniques: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.Actomyosin: A protein complex of actin and MYOSINS occurring in muscle. It is the essential contractile substance of muscle.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Kinetics: The rate dynamics in chemical or physical systems.Cell Cycle Proteins: Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.Polymers: Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Fungal Proteins: Proteins found in any species of fungus.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.cdc42 GTP-Binding Protein: A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS. It is associated with a diverse array of cellular functions including cytoskeletal changes, filopodia formation and transport through the GOLGI APPARATUS. This enzyme was formerly listed as EC 220.127.116.11.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Depsipeptides: Compounds consisting of chains of AMINO ACIDS alternating with CARBOXYLIC ACIDS via ester and amide linkages. They are commonly cyclized.Muscles: Contractile tissue that produces movement in animals.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Protein Isoforms: Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.Molecular Weight: The sum of the weight of all the atoms in a molecule.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Microtubules: Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.Wiskott-Aldrich Syndrome Protein, Neuronal: A member of the Wiskott-Aldrich syndrome protein family that is found at high levels in NERVE CELLS. It interacts with GRB2 ADAPTOR PROTEIN and with CDC42 PROTEIN.Biopolymers: Polymers synthesized by living organisms. They play a role in the formation of macromolecular structures and are synthesized via the covalent linkage of biological molecules, especially AMINO ACIDS; NUCLEOTIDES; and CARBOHYDRATES.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Wiskott-Aldrich Syndrome Protein: WASP protein is mutated in WISKOTT-ALDRICH SYNDROME and is expressed primarily in hematopoietic cells. It is the founding member of the WASP protein family and interacts with CDC42 PROTEIN to help regulate ACTIN polymerization.Parathyroid Hormone-Related Protein: A ubiquitously expressed, secreted protein with bone resorption and renal calcium reabsorption activities that are similar to PARATHYROID HORMONE. It does not circulate in appreciable amounts in normal subjects, but rather exerts its biological actions locally. Overexpression of parathyroid hormone-related protein by tumor cells results in humoral calcemia of malignancy.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Nerve Tissue ProteinsCytoplasm: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.rho GTP-Binding Proteins: A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 220.127.116.11.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Arabidopsis Proteins: Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.Cytochalasins: 11- to 14-membered macrocyclic lactones with a fused isoindolone. Members with INDOLES attached at the C10 position are called chaetoglobosins. They are produced by various fungi. Some members interact with ACTIN and inhibit CYTOKINESIS.Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Myosin Type II: The subfamily of myosin proteins that are commonly found in muscle fibers. Myosin II is also involved a diverse array of cellular functions including cell division, transport within the GOLGI APPARATUS, and maintaining MICROVILLI structure.Dictyostelium: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.Microscopy, Confocal: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).COS Cells: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Adaptor Proteins, Signal Transducing: A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymesSequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Intracellular Signaling Peptides and Proteins: Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Filamins: A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.Muscle Proteins: The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Nucleic Acid Synthesis Inhibitors: Compounds that inhibit cell production of DNA or RNA.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Protozoan Proteins: Proteins found in any species of protozoan.Structural Homology, Protein: The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Vinculin: A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Wiskott-Aldrich Syndrome Protein Family: A family of microfilament proteins whose name derives from the fact that mutations in members of this protein family have been associated with WISKOTT-ALDRICH SYNDROME. They are involved in ACTIN polymerization and contain a polyproline-rich region that binds to PROFILIN, and a verprolin homology domain that binds G-ACTIN.Viscosity: The resistance that a gaseous or liquid system offers to flow when it is subjected to shear stress. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Bacterial Proteins: Proteins found in any species of bacterium.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Tissue Distribution: Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Cell Surface Extensions: Specialized structures of the cell that extend the cell membrane and project out from the cell surface.rhoA GTP-Binding Protein: A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 188.8.131.52.Precipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Calmodulin-Binding Proteins: Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.Caenorhabditis elegans Proteins: Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.Cell Line, Tumor: A cell line derived from cultured tumor cells.rac1 GTP-Binding Protein: A rac GTP-binding protein involved in regulating actin filaments at the plasma membrane. It controls the development of filopodia and lamellipodia in cells and thereby influences cellular motility and adhesion. It is also involved in activation of NADPH OXIDASE. This enzyme was formerly listed as EC 220.127.116.11.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.rac GTP-Binding Proteins: A sub-family of RHO GTP-BINDING PROTEINS that is involved in regulating the organization of cytoskeletal filaments. This enzyme was formerly listed as EC 220.127.116.11.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.Proto-Oncogene Proteins c-bcl-2: Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.Electrophoresis, Gel, Two-Dimensional: Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Drosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.Tubulin: A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Cytochalasin B: A cytotoxic member of the CYTOCHALASINS.Myosin Type I: A subclass of myosins found generally associated with actin-rich membrane structures such as filopodia. Members of the myosin type I family are ubiquitously expressed in eukaryotes. The heavy chains of myosin type I lack coiled-coil forming sequences in their tails and therefore do not dimerize.Microtubule-Associated Proteins: High molecular weight proteins found in the MICROTUBULES of the cytoskeletal system. Under certain conditions they are required for TUBULIN assembly into the microtubules and stabilize the assembled microtubules.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.GTPase-Activating Proteins: Proteins that activate the GTPase of specific GTP-BINDING PROTEINS.Cross-Linking Reagents: Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Protein Multimerization: The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.Cytokinesis: The process by which the CYTOPLASM of a cell is divided.Muscle, Skeletal: A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.RNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.Schizosaccharomyces pombe Proteins: Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Schizosaccharomyces: A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.Myosin Type V: A subclass of myosin involved in organelle transport and membrane targeting. It is abundantly found in nervous tissue and neurosecretory cells. The heavy chains of myosin V contain unusually long neck domains that are believed to aid in translocating molecules over large distances.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Cell Adhesion Molecules: Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.Colipases: Colipase I and II, consisting of 94-95 and 84-85 amino acid residues, respectively, have been isolated from porcine pancreas. Their role is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Luminescent Proteins: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.Retinoblastoma-Like Protein p130: A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. RBL2 contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and E2F5 TRANSCRIPTION FACTOR. RBL2 also interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Protein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Cercopithecus aethiops: A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.Tropomodulin: An actin capping protein that binds to the pointed-end of ACTIN. It functions in the presence of TROPOMYOSIN to inhibit microfilament elongation.Guanine Nucleotide Exchange Factors: Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.NIH 3T3 Cells: A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)


text:
Beware – Candidate shortages Ahead!
Business Owners and hiring Managers need to prepare for a challenging year in 2019. The job portal Adzuna has revealed that competition for roles is at an all-time low, since starting their stats in 2012. The researchers found there are currently only three vacancies per jobseeker, indicating that the fight for talent is going to […]Read More
Do you dread the Office Christmas Party?
The work Christmas party can be a wonderful time to 'let your hair down' with your colleagues, meet their partners and generally have a great night out. Sometimes though, spending time outside of work with your work colleagues is not everyone's idea of fun, but if done correctly, can really help bond employee and employer […]Read More
How is your Company Culture?
It has long been known that staff are a Company's greatest asset and that Company culture is a huge contributor to both staff performance and recruiting attraction. But when we try to discuss Company Culture, it is often put to one side and dismissed and deemed not as valuable as others aspects of the role […]Read More
Are you losing sleep over work?
Whilst the build up to Christmas is often a very busy time for Business', over the Christmas and New Year break, people get a chance to pause with Family & friends whilst away from work, often spending time reflecting on last year and making decisions for the coming one. It is very clear that the […]Read More

subset: commoncrawl


text:
In-DEX Yard Management technology, when used in conjunction with In-DEX WMS Warehouse Management Software, allows users to efficiently schedule inbound and outbound loads evenly across days and in doing so optimises movements with available resources such as loading bays, operators, handling equipment and more.
As well as being a shared diary, In-DEX WMS Yard Management automatically sends email confirmations with the agreed appointment details to customers, suppliers and transport operators along with a 2-D barcode.
When vehicles are running behind their advised booked times, In-DEX WMS Yard Management can automatically send an email alert following a user-definable time period requesting that the transport company contacts their driver to call with an updated arrival time.
For business efficiency analysis, against transport company, customer or supplier KPI reports can be produced to analyse actual times against the booked times.
Booking Slot Confirmation Process
Vehicle Site Pass Process


text:
With the Law Library now available to attorneys, the Attorney Lounge in the Page Belcher Federal Building is being repurposed as of May 2. Attorneys will still have a welcoming place to check email or simply relax while waiting for court to resume. The Library, which was renovated last year, offers access to Wi-Fi, use of a conference table, and a lounge area. The TV with cable access that was located in the Attorney Lounge, will be moved to the Library.
Location and Hours of Operation: The Page Belcher Federal Building is located at 333 West 4th Street, Tulsa, OK. The Court Clerk's Office and the Law Library are located on the 4th Floor, and are open Monday through Friday, 8:30 a.m. to 4:30 p.m., excluding Federal Holidays.


text:
The regional awards season continues – just days after the announcement of the Queenstown winners, the Rotorua finalists have been revealed. Presented by the Restaurant Association of New Zealand, the Rotorua Hospitality Awards recognise the outstanding hospitality professionals all making a positive impact on the local hospitality scene. A large number of nominations were received from the industry across 16 categories, recognising those who work hard to achieve excellence in hospitality.
Marisa Bidois, Chief Executive of Restaurant Association says:
"Time and time again we are impressed by the number of nominations received and we see this as an endorsement that the Rotorua hospitality industry welcomes the awards as a means to support and recognise each other. It is a real tribute to the calibre of talent in Rotorua hospitality. To be selected by your peers as a leader in your category is a great honour in itself."
Winners will receive a coveted "Chevron" trophy, representing a stripe indicating rank or length of service. Winners will also be listed on the Eat New Zealand website, a movement dedicated to promoting New Zealand's best food, drink and culinary destinations to the world.
Final voting via the website is now open to the industry, closing at 5.00pm on Sunday, 24 June 2018.
The public can also have their say and vote for their favourite establishment via the Rotorua Lakes Council People's Choice Award. This will be awarded to an establishment that excels in customer service, consistency in food preparation and delivery, welcoming ambience and style, attention to detail and is always willing to go that extra mile. Online voting is also via the website for both industry and the public.
The winners will be announced at the awards evening, held at the Energy Events Centre on Sunday, 1 July. The Rotorua Hospitality Awards is your opportunity to get together with the city's hospo squad, enjoy a night of delicious canapés, wine, beer, and cocktails, and participate in a bit of healthy competition at our awards ceremony.
2018 Rotorua Hospitality Awards Finalists
More FM Rotorua Outstanding Barista
Ethan Butler, Artisan Cafe
Leroy Cunningham, Market Kitchen
Grace Choi, McDonalds
Andrea Baigorria Germain, Revolver Espresso
Bidfood Rotorua Café
Artisan Café
Lime Caffeteria
Scope Cafe
Restaurant Association of NZ Outstanding Bartender
Sean Kelly, Atticus Finch
Shaye Honeycombe, Ponsonby Rd Lounge Bar
Rohi Mehla, Rydges Rotorua
Scott Barlow, Stratosfare Restaurant and Bar
Lion Breweries Bar of the Year
BREW Craft Beer Pub
Ponsonby Rd Lounge Bar
Pig & Whistle Historic Bar
Hospitality Management Systems Front of House Team
Atticus Finch
Indian Star Tandoori Restaurant
Stratosfare Restaurant and Bar
Tabasco Emerging Talent – Chef
Bethany-May Allen, Peppers on the Point – Lake Rotorua
Pritam Gaware, Skyline Rotorua
Gill Fursharan, Urbano Bistro
Toi Ohomai Institute of Technology – Outstanding Chef of the Year
Phil Elliot, Atticus Finch
Deep Rajput Thakur, Giovannis Restaurant
Digraj Baghela, Princes Gate Hotel
Mark Solon, Urbano Bistro
Indian Star Emerging Talent – Business
Delhi Hutt
El Mexicano Zapata
Scope Cafe
OneMusic Outstanding Ambience & Design
Ponsonby Rd Lounge Bar
Regent of Rotorua
Urbano Bistro
Restaurant Association of NZ Outstanding Ethnic Restaurant
Indian Star Tandoori Restaurant
The Thai Restaurant
Yamato Japanese
Eftpos NZ Outstanding Restaurant
Atticus Finch
Regent of Rotorua
Urbano Bistro
GrabOne Outstanding Takeout
24b Lunchbar
Pizzeria Imperfetto
The Factory Smokehouse & Grill
Skyline Rotorua Outstanding Sales Rep
Rohan Knowles, Diverse Distributors
Donna Bloore, Jasco Distributing
Tracey Lawrence, Service Foods
Restaurant Association of NZ Outstanding Supplier
Bidfood Rotorua
Diverse Distributors
Service Foods
Events & Venues Excellence in Event Hospitality
Crankworx Rotorua
Rotorua Night Market
The Okere Falls Beerfest


text:
This party season get fun, funky and fabulous and choose from a gorgeous range of party essentials from Miso. The Miso girl is young, bright and full of personality, as well as being fashion forward and knows how to style prints, patterns and statement clothing whilst still retaining a feminine edge.
Sparkle the night away and be the envy of all your friends in a show stopping Sequined Peplum Dress, or be fun and flirty in this seasons must-have Mesh Panel style. Just don't forget to accessorise with big hair, big eyes and killer heels.
This Peplum Mesh Panel Dress is a bold statement dress for the festive season. This dress is designed to show off your curves to perfection. With a trendy peplum hem and a sexy black mesh panel, you can dress to kill this winter. Wear with black opaque tights and accessorise with a clutch bag and statement jewellery to complete the look.
Get ready to party by investing in this fabulous dark red Sequined Peplum Dress. With sweetheart neckline, peplum waist feature and adorned head to toe in red sequins, this is a glamorous party dress that will make sure you stand out from the crowd!
Click on one of the images to see the full range.


text: The increasing popularity of the double oven in Australian kitchens is being linked to an ever-growing passion for home cooking and the fact that, in general, our kitchens are larger than those of Europe and the UK. And while two ovens are often better than one, one leading supplier is advising consumers to ensure they really are getting two ovens and not just a poor imitation of the very versatile genuine double oven. Smeg Australia says a double oven should provide two separate ovens that each offer fan baking, static baking and a separate grill. The appliance should also provide less traditional functions such as pizza cooking and energy saving programs.One of the most versatile double ovens on the market is Smeg's DOSPA38X, a richly featured appliance that offers eight fan and static cooking functions in the main oven (79 litre capacity), four in the second(41 litre capacity) plus two pyrolytic cleaning modes.The auxiliary oven has a recessed floor for an optional pizza stone,which means users can achieve a perfectly crisp and brown pizza base with an evenly cooked topping.The ECO energy saving programs comprise a cooking mode (in each oven) whereby the grill and lower element are used in combination with the fan to provide efficient, even heat distribution while conserving energy.The ECO pyrolytic cleaning program meanwhile, in the main oven,runs for 90 minutes at an interior temperature of 500°C, carbonising food residue to a fine ash – ideal for lightly soiled ovens. This program uses up to 50 per cent less energy than the regular pyrolytic cleaning program for heavily soiled ovens.The Classic satin stainless steel DOSPA38X double oven is a signature design by renowned Italian architect, Guido Canali – lines are beautifully clean and understated and the styling is absolutely timeless. Standard features include LCD panel, manual or programmable cooking, thermoseal oven technology and a fingerprint-proof stainless steel surface. The energy rating of this appliance exceeds the European Energy Rating A by 20 per cent.


text:
Every year about this season some theatre company present "It's a Wonderful Life: A Live Radio Play" which is based on the Frank Capra film of 1945. WMTC, a Manhattan radio station, is presenting on Christmas Eve 1946 an adaption of the classic film that starred Jimmy Steward, Donna Reed and Lionel Barrymore.
Watching this excellent production that featured five gifted actors it brought back memories of the 1950's "radio plays" that I saw in the Hollywood area. The major movie studios would present studio contract players to do radio adaptations of movies. The major one was the "Lux Radio Theatre" on Monday nights.
A little of the history of this particular radio play if I may. About 20 years ago, Frances Kondziela, a longtime friend and teacher asked playwright Joe Landry to pen an adaption of the iconic play for her high school ensemble. After the high school premiere, TheatreWorks of New Milford, Connecticut, staged a full production at the Westport County Playhouse. Since then many regional companies have presented this holiday classic to appreciative audiences and their families.
The story is as familiar and comfortable as a pair of old pajamas. The cast performs with scripts in hand in front of old fashioned silver microphones with a large Christmas tree off to the left side of the stage. The audience sees on how a radio show is produced with various props for sound effects that are handled by the actors and two signs overhead that say "Applause" and light up at the appropriate times. The fourth wall is virtually disregarded and a certain intimacy between the actors and the audience is inevitably forged.
Aaron Murphy plays George Bailey the Jimmy Stewart role in this play. He has an engaging presence playing the "good guy". Joseph Kearns is exceptional playing the "bad guy" Potter along with Uncle Billy and various other characters. Justin Gillman is splendid in all of his characterizations that included Harry and young Harry, Clarence and Dr. Campbell. Hanna Finn does a fine job on her portrayals of Mary Hatch and young Mary. Rounding out the cast is Hillary Hesse who strikingly preforms over ten characters.
"It's a Wonderful Life: A Live Radio Play" plays through December 28 at the Tides Theatre, 533 Sutter Street, San Francisco. For tickets call 415-399-1322 or on line at www.tidestheatre.org


text:
Applying Sealant: Waterproofing Your Basement
Applying waterproofing sealant to your basement is important to avoid problems moisture problems. These problems can cause property damage and lower the value of you home. Water damage can cause molds and mildew that is why it is important to waterproof such areas of your home. Prior to applying sealant in your home, you should repair holes and cracks first as you do not want to seal damaged walls and floors.
Step 1 - Locate Leaks
Locating leaks in important to know where the damage has begun in our basement. It is easy to spot these leaks because you can see moisture and mold buildup in these areas at the late stages. During early stages of, you may see puddles forming or visible water build up. These leaks are often found on wall and floor joints.
Step 2 - Clean Mold Buildup
Mold appears to be this black and fuzzy growth on walls and other porous materials such as paper, cardboard, wood and carpets. Basements tend to be storage for most people so you have to clean this area prior to sealing. You do not want to seal in mold. Remove anything that has developed mold or items will probably develop mold in the future from under your basement. If your insulation has developed mold, it is best to replace it as mold will continuously replicate if not eliminated. Use soapy hot water or an antifungal cleaner to clean the walls and floors. Use a heavy-duty brush for stubborn mold. Areas that appear to not have mold should also be cleaned as this may have spores developing on them as well. Make sure you are wearing your protecting mask and goggles when you are dealing with mold as they can cause respiratory problems.
Step 3 - Dehumidify Area
After cleaning, dry the area with a cloth and switch on dehumidifier. This will help stop the development of mold. Initiate preventive measures by dehumidifying your basement frequently.
Step 4 - Patch Leaks
One the area has bean cleaned and dehumidified, repair all leaks and seepages. Avoid repainting, as sealant will not stick to paint. It is best to sand areas that have been painted to ensure it has a surface that sealant will adhere to.
Step 5 - Mix Sealant
Latex waterproofing sealants or epoxy is available in your local hardware. Always follow the mixing instructions on containers.
Step 6 - Apply Sealant
Patch your wall surface section by section until you have covered the whole area. Make sure that you take time to push in the sealant into the walls little crevices and corners. Apply more on areas that are prone to leaks and seepages such as areas to you have repair earlier. As soon as the first coat has dried, moisten the area with a fine mister and then let it sit overnight. Apply a second coating but make sure you mist it again prior to second coating.


text:
Debt-ridden couple refused bankruptcy in England
Posted: 27th June 2013
A couple who are facing debts in excess of €70 million in Ireland following the collapse of their property investment business have failed to convince the Court of Appeal that they should be declared bankrupt in England on the basis that they have established their centre of main interest (COMI) in this country.
Prior to the demise of Ireland's 'Celtic tiger' economy, the couple had amassed a property empire that spanned the world, with office and retail buildings in Dublin, London, Stockholm and Washington DC. Their assets included a mansion near Dublin, a country estate in Galway, a holiday home in the French Alps, a substantial home in central London and a collection of classic cars.
Their principal creditor, the Bank of Ireland, obtained a judgment against them at the Irish High Court in 2010 fo €71.5 million, plus costs. The couple moved to London shortly afterwards and were accused of 'bankruptcy tourism' by the bank which claimed that they were attempting to take advantage of what they perceived to be the more benign debt recovery regime prevailing in England.
The High Court in London subsequently rejected the couple's plea that they had established their COMI in England and dismissed their bankruptcy petitions. The Court found that, so far as their creditors were concerned, their business operations always had been, and remained, based in Dublin.
In the Court of Appeal, the couple argued that it had not been necessary for them inform each and every one of their creditors of their change of COMI. It was submitted that their settlement in England was evidenced by their regular shopping trips to Fortnum & Mason, visits to the capital's theatres and a plot that they had reserved for themselves at Westminster's Mill Hill Cemetery.
Refusing permission to appeal, the Court ruled that any challenge to the judge's decision would have no reasonable prospect of success. The judge had taken an entirely conventional approach to the law and criticisms of his findings of fact were not a proper basis on which to mount an appeal. The Court also declined to refer the matter to the European Court of Justice.


text:
There's nothing more classic than a pale blue button down. But we've taken it up a notch with a swiss dot print for a subtle hint of flair. This style perfectly blends timeless and a modern for a look that works for everyone.
Free shipping on orders over $99. If it doesn't fit, we'll refund or exchange it at no extra fees.
Buy 2 Shirts for $100.
Buy 3 Shirts for $140.
We're here daily,9 a.m.–9 p.m. firstname.lastname@example.com


text:
ATΩ Delta's 148th Anniversary Reunion
October 14 – 15, 2016
Calling all Taus – clear your calendars for the weekend of the Pittsburgh football game this fall and come to Charlottesville to celebrate the 148th Anniversary of the chartering of ATΩ Delta! Oh, and it's also Homecomings Weekend at The University!
Schedule of Events:
- Friday, October 14
- 6:30-8:30pm: Dinner at in the Newcomb Hall Ballroom
- 9:00pm: "Beer Bash" as 125 Chancellor Street featuring The Bootsie Daniel Band
- Saturday, October 15
- TBD: Tailgate in the Pavilion I Garden.
- TBD: Pittsburgh vs Virginia football game.
- TBD: Saturday night band party.
We have a block of rooms at each of the hotels below. Make sure to use the phone number listed, the national hotel reservation number may not be able to access our block of rooms. Ask for the "UVA Alumni Association ATO Event" block.
Cavalier Inn (434-296-8111) — $189
Hyatt Place (434-995-5200) — $189
Hampton Inn 29 North (434-978-7888) — $159
Fairfield Inn (434-964-9411) — $159
La Quinta (434-293-6188) — $159
Please let your fellow Taus know you'll be in attendance by RSVP'ing below.
We hope to see you this fall for what should be our biggest reunion yet!


text:
Alpine Toilets
This is a double toilet package with the building constructed from colour steel sandwiched foam. It is designed to fit over a 4000L holding tank and comes complete with a super vent and roto Vent.
This alpine toilet is very light, robust and easy to clean. Doors can be inward or outward opening and are fitted with door closers, privacy latches, and heavy duty stainless steel door handle sets.
The floors are aluminium checker-plate and carry a slight slope to the door for easy cleaning. The building has an opaque fibreglass skylight, and a partition wall.
Due to demand we have been manufacturing some double buildings of different configurations, for example fully accessible cubical and a standard single cubical in one building and two fully accessible cubicles in one building. Double buildings have a partition wall between and one skylight.
A stainless steel hand basin fitted and supplied with an industrial spring action tap is an optional extra.


text:
We here at Atheist Analysis don't usually create formal response letters to blogs or news stories unless they are of immense social and humanistic consequence, but recently a link was given to me that lead to a progressive christian blog. This more compromising version of faith is, to me, less harmful and overall a step in the right general direction, while still providing enough material for all of us here on the blog team to continue picking apart the hypocrisy; it's the cutting off heads and hellfire damnation that is lacking – for the better, most would concede.
For this short reply blogger Moonlit History and I, Deafilosophy (or Chris Hanna as there are a lot of pseudonyms being thrown around at the moment), will be commenting on some of the points, perspectives, open-ended questions, and conclusions made in the article linked above. So without further ado, I will begin.
Aside from the horrendous grammar in the title of the article at hand, or, more accurately, the lack thereof, I was initially quite content with just perusing the content with a smile as any time people of faith accept science over empty pseudo-superlatives I get all warm and fuzzy inside. But, that title just ate at me. Let's try, "Religion and Science: Can We Talk?" instead. There, isn't that better?
As an engineer and open atheist almost all my life, I did not know atheism had a name until high school. I am quite familiar with most of the apologetic and progressive arguments for God that absorb scientific explanations. Immediately the fine tuning argument is casually implied with an invocation of the cosmological constant, and, of course, mentioning Albert Einstein, a noted Spinozan deist at best.
Two things and then I will give the floor to my esteemed colleague; the fine tuning argument is the most basic argument for the prime mover, for classical deism, and it is also the limit of our understanding of the universe at the moment. But using this argument to prove the personal Christian God is to overextend and ultimately, as C.S. Lewis was so apt to do, try to prove too much with too little:
"Sigmund Freud wrote that the voice of reason was small, but very persistent. C. S. Lewis tried to prove too much by opining that the presence of a conscience indicated the divine spark" (Hitchens, "god is not Great," 2007, p. 256).
Continue reading "A response to "Religion and science can we talk?""


text:
Two and a half months into the New Year, we've gotten rather used to 2016. I'm no longer writing "2015" on documents out of habit; it's been long enough. The habit has changed. We're long enough into the New Year that the new behaviors we promised ourselves at the turn of the year should be ingrained in us. They should be – but are they? Those of us who practice Lent may be in the midst of new behaviors promised at Ash Wednesday. Now is a good time to review our New Year's Resolutions, if we made them. How are we doing?
Personally, I decided this year that New Year's Resolutions as traditionally practiced were not useful to me. For me, they have been unreasonable promises to make sweeping changes in behavior or high, unattainable expectations for living conditions. So, instead of making promises to myself that I could not keep, I spent some time at the end of 2015 pondering what I needed most in my life. From there, I sought one word that I could focus on this year. I sought a word that would encompass everything that I felt I needed to accomplish in 2015 in my physical as well as my spiritual life. By December 31, the word I came up with was "Organization." I thought if I could clean out my closets, literally as well as figuratively, throughout this next year, life would improve.
Organization. It was a good choice. It covered everything I had thought of that I needed to work on. It's a good, solid word. It's a utilitarian word that describes rearrangement and cleaning out. Yes, it was good, I decided, and I began the year with this word as my focus: Organization.
Then came the final meeting of my Simple Abundance group. We had meeting been almost monthly throughout 2015 to discuss our readings in the classic devotional by Sarah Ban Breathnacht. As a group, we had been learning to apply spirituality to our daily lives through the readings and our discussions. We were unable to meet at the end of 2015, so we met on the first weekend in 2016 to wrap up our discussions and to discover our intentions for the new year. Obviously, I intended to share my thoughts about Organization. It had, in fact, been our Simple Abundance reading and discussion that had led to my choice of this focus word for the year. By the time we came to our sharing about the coming year, though, something changed.
You see, after we talked about the readings and how we had been affected by them over the year, we spent some time in meditation. Our leaders asked us to think about a word or a phrase that would encompass our goals for the coming year. Of course, I had done this. I knew my word. Organization.
Our gatherings took place at a beautiful place in Apache Junction, Arizona called Sacred Space for Retreat. The property includes a large stone labyrinth. We were sent to the labyrinth for a silent meditative walk. The labyrinth walk is one of my favorite contemplative practices. In fact, I have a small labyrinth of my own at home. The one at Sacred Space is much larger and provides the opportunity for a longer, deeper walk with God.
As I walked that day, focusing at first on my word, "Organization," I began to let go of thought, focusing only on my breath and my steps. As I neared the center, I began to feel cleansed. I looked at the ground for a moment as I walked and I noticed a geode. I came to the center, where I stood facing the eastern mountains. I took three deep belly breaths, and stood in silence. I could hear the soft crunching of the feet of those who walked before and after me. I heard the birds that flew above and the rustling of leaves on the mesquite that grow in this desert environment. I felt the caress of the soft breeze upon my face, upon my uplifted hands.
Then, I heard clearly in my mind a new word: Purity.
As I walked out of the labyrinth, I pondered this word. In today's world, the word "purity" can have connotations that feel contrived or self-righteous. The first meaning of this word that came to me was about the idea of moral purity, which seems to me a rather subjective connotation. Among the evangelical crowd, there is a push for sexual purity that leads to purity contracts, purity balls, purity rings; all promises to remain "pure" until marriage. A second, more negative connotation of the word "purity" is related to the idea of racial purity and the atrocities of ethnic cleansing. However, to be pure is also to be free of harmful substances; it is to be clean, as in purity of water or air; it is to have a perfect quality, as in the purity of sound, music, voice.
Thinking about this word as I walked out of the labyrinth, I wondered how it could apply to me or to my life. I am a messy human being. I am disorganized, often scattered, and always overscheduled. In other words, I am a human being immersed in twenty-first century life. What has "purity" to do with me?
I thought of the geode. On one side, the geode is a rather ugly, bumpy, dirty rock. On the other, the stone is white and almost translucent in its beauty. One can imagine that beneath that lovely, smooth, shiny surface, there could be some glorious crystal.
Many years ago I read a science fiction novel that I will never forget. The book, Swan Song, by Robert R. McCammon, describes the world after a nuclear detonation. The radiation spurs an evolution among the people that begins with the growth of what they call "Job's Mask," which covers their faces. The masks are ugly, hard, and often debilitating crusts. Some of the people develop gifts as well, such as visions and the ability to make things grow. Eventually, the Job's Masks fall off, revealing the true nature of the individuals inside. Some who had been considered ugly, bad, or unwanted before the war are revealed to be truly beautiful on the inside.
As I pondered the geode, I thought of this book, and I thought of what both the rock and the book revealed to me about purity. Purity is not always visible. Alchemical purity is the absolute perfection of a substance. As a person, is purity truly possible? As a Wesleyan, I have to say that yes, it is. After all, Purity is Perfection. Perfection is possible. It is not something to declare; it is something to seek. It is not something to take pride in; it is something to be revealed. If Purity is cleanliness and the absence of harmful substances, then "Organization" is an activity to be used as a tool toward Purity, toward Perfection.
As we near the final week of Lent, we commemorate the clownish street theatre of Jesus' final entry into Jerusalem on an ass, with palms waving and strewn on the ground before his feet. We remember the final week the twelve Apostles and the many Disciples would have with the friend and teacher they knew as Jesus, the night they shared a final meal, and the night He would be betrayed and denied. This week, we near the end of our 40 days of self-denial and contemplation.
On Easter morning, we remember the stone rolled away to reveal an empty tomb and Jesus transformed into the glorious, divine Christ. We will also be rolling aside our Lenten promises. When we do, what will we reveal? Will we be new people, set with our faces toward resurrected lives in Christ?
It is my prayer that on Easter morning I will set aside my simplistic denial of a worldly thing and continue the formidable task of organizing my cluttered life, revealing beneath its ugly, bumpy reality the hidden path toward purity. It's only one new beginning of many new beginnings winding through my life. Yet, each new beginning is an opportunity to set aside the ugliness of self-absorption to reveal the deeper beauty found in self knowledge.
My path toward Purity begins with Organization.
There's an app for that.
I call it prayer, followed by action.


text:
© Ryan Liebe
This delicious popcorn is flavored with a mix of Japanese seasonings like togarashi (a chile blend) and crumbled nori (dried seaweed). Slideshow: Make-Ahead Snack Recipes
How to Make It
In a food processor, pulse the first four ingredients to a fine powder. Toss with buttered popcorn and serve.


text:
Back to Journals » Hepatic Medicine: Evidence and Research » Volume 8
Obeticholic acid for the treatment of primary biliary cholangitis in adult patients: clinical utility and patient selection
Authors Bowlus C
Received 14 June 2016
Accepted for publication 22 July 2016
Published 1 September 2016 Volume 2016:8 Pages 89—95
DOI https://doi.org/10.2147/HMER.S91709
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Dr Gerry Lake-Bakaar
Christopher L Bowlus
Division of Gastroenterology and Hepatology, University of California Davis, Davis, CA, USA
Abstract: Primary biliary cholangitis (PBC), previously known as primary biliary "cirrhosis", is a rare autoimmune liver disease characterized by the hallmark autoantibodies to mitochondrial antigens and immune-mediated destruction of small bile duct epithelial cells leading to cholestasis and cirrhosis. Surprisingly, while immune modulators have not been effective in the treatment of PBC, supplementation with the hydrophilic bile acid (BA) ursodeoxycholic acid (UDCA) has been demonstrated to slow the disease progression. However, a significant minority of PBC patients do not have a complete response to UDCA and remain at risk of continued disease progression. Although the mechanisms of action are not well understood, UDCA provided proof of concept for BA therapy in PBC. Obeticholic acid (OCA), a novel derivative of the human BA chenodeoxycholic acid, is a potent agonist of the nuclear hormone receptor farnesoid X receptor, which regulates BA synthesis and transport. A series of clinical trials of OCA in PBC, primarily in combination with UDCA, have established that OCA leads to significant reductions in serum alkaline phosphatase that are predicted to lead to improved clinical outcomes, while dose-dependent pruritus has been the most common adverse effect. On the basis of these studies, OCA was given conditional approval by the US Food and Drug Administration with plans to establish the long-term clinical efficacy of OCA in patients with advanced PBC.
Keywords: primary biliary cholangitis, nuclear receptors, farnesoid X receptor, bile acid, obeticholic acid, ursodeoxycholic acid
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]


text:
Fax +49 (911) 179 3736
Dr Möller's research centres on: incentive systems, the organization of work, personnel economics, and the organization and coordination of research issues.
From 1988 to 1991, Dr Möller received her first vocational training in a bank (Bankkauffrau). In 1999 she completed her degree in social sciences at the University of Erlangen-Nuremberg (including a study visit to Högskolan in Örebro, Sweden). She has been a Senior Researcher at the IAB since 2000. In 2007 she was awarded her doctorate in economics (title of thesis: "Organization, Personnel Policy and Company Performance").


text:
Need To Do Laundry?
You just need us! Let us take care of it for you today.
Don't waste hours you don't have just to get your laundry done. Bring it to The Spin Cycle Laundry & Tanning and let us take care of it for you - every step of the way!
We'll wash and dry all your laundry. We take the time to read the labels, separate it for you, and make sure that your fabrics are all treated exactly the way they should be.
You have access to tons of drop off laundry services, including folding, ironing, pressing, and more! Just let us know what you need and we'll take care of it for you.
You don't even have to come in to let us get to work on your laundry! You can simply use our drive-thru window for quick and easy drop-off.
Don't have time to visit us? Not a problem! Just give us a call and we'll pick it up from your home or business. It couldn't be easier!
You can also use our laundry center for your large capacity items, or stop by and take advantage of our modern tanning beds.


text:
Portable Car Lifts
ASEplatinum Portable Car Lift MR6K-38
ASEplatinum MR6K-38 Portable Mid-Rise Car Lift | 6,000 lb. Capacity Mid-Rise lift is built to last and a remarkable value
ASEplatinum MR6K-48X Mid-Rise Scissor Lift
ASEplatinum MR6K-48 Scissor Lift, 6,000 LB Capacity Perfect lift for raising automobiles like typical passenger cars and light trucks. For brake repairs, car detailing, wheel and tire repiars or ...
Automotive repairs have never been easier with this truly unique portable car lift. Just roll it up to the vehicle with it's pallet jack style design, and then lift the car up to a full rise height so you can comfortably stand up underneath the car to perform service. Then just roll it away when you're done. It's that easy. This commercial grade hoist has a 1 year warranty.


text:
Shopping in Lidl has unexpected surprises. The supermarket seems to take the same "stack 'em high, sell 'em cheap" approach that characterised Kwik-Save (RIP 2007). But with the added frisson of eccentric one-off items.
I can see that competition with Adsa (Wal-Mart ) and Tesco means they'd want to sell non-grocery goods. Recent items include fishing tackle, hall furniture, and laptop computers. We noticed that motorcycle gear was popular, and that skiiing underwear seemed to take a long time to shift from the shelves.
But under what conceivable circumstances would a Lidl shopper make an impulse buy of this week's random bargain: unicycles? I like to imagine some of the more adventurous customers proudly cycling home with their new purchase. At least they'd have both hands free to carry the rest of their shopping.
It's amazing what Lidl think they can peddle to their customers, eh?


text:
Djwhal Khul Spirituality Article
Channeled by Rev. Terri Newlon
(Complimentary every week)
"Venus Joins Mars in Virgo"
August 22, 2019
(Channeling begins)
Djwhal Khul here. Tashi Delek.
Alright. Well, we had Mars moving into Virgo on August 17 and now we have Venus having joined Mars in Virgo as of August 21 and then of course the Sun going into Virgo.
So I'm seeing a triple power of that Virgo energy which is rather dynamic, it's logical, it organizes things, it looks at step by step plans, it looks at end result and then plans backwards to achieve the end result, so I think this Virgo energy is quite good.
By the time we get to August 29, we will also have Mercury the communicator and the messenger of the Gods also entering Virgo. So this Virgo strong energy I'm liking very much.
As always, thank you and my love to you.
Djwhal Khul
Channeled by Rev. Terri Newlon
(Spirituality Article, Transcribed by Micheline Ralet)


text:
Redefining Precision and Control, Blue Key World Offer Affordable Chef Knife
Portland, OR -- (SBWire) -- 03/09/2017 --A chef's knife comparable to the best knives in the world, Blue Key World has been able to impress their buyers with their 8 Inch Chef Knife. The company promises out of the box sharpness, and an easy to use and clean, ergonomic design that is perfect for most hands that has been cleverly achieved through an innovative technique that makes Blue Key World's stainless steel chef knife one of the best on the market for the price.
Speaking how they were able to achieve such an amazing feat, the spokesperson for Blue Key World said, "We at Blue Key World so cleverly created our outstanding knife that is good for all hands, very easy to use and clean with a super quality 8'' stainless steel blade almost on par of those $200 knives that Michelin star chef's talk about." He further added: "Here's what we did, started contacting 24 different manufacturers whose specialty is in creating kitchen knives. We contracted out models from each and one of them and were happy with none of them. However, there were kernels of greatness in some of our prototypes. We analyzed and pulled the best elements out of each - and infused them into the Blue Key World's knife you see before you."
It took the company several months to develop a product that they were confident about. The Blue Key World is determined to keep providing their customers with new products that make their lives easier.
An extremely satisfied customer Scott from NH wrote in his testimonial: "Very happy with the knife. The First use was to cut veggies for stir fry. It was even better than the knife we bought at a Chinese cooking class for that purpose. We bought it to replace a 60 year old kitchen knife that finally fell apart. Didn't think we'd find something we liked as well, but this one is a perfect replacement. We like how light weight it is. This knife is razor sharp and we will look into buying the sharpener to keep it that way. We plan to look for more knives from the company."
To purchase and learn more about Blue Key World 8 inch chef knife, please visit: https://www.amazon.com/Chef-Knife-Multipurpose-Stainless-Restaurant/dp/B01A8R6LHA
About Blue Key World
Blue Key World is a brand and retailer of innovative consumer products on Amazon.com, the brand was established in 2014. The aim of the brand is to deliver products that make lives easier for their customers and also saves time.
Media Contact Information:
Luis Amaral
Phone Number: 503-568-1586
Address: 1812 N Columbia Blvd, Suite C15-249747
Portland, Oregon, 97217, USA
Website: http://www.bluekeyworld.com
Media Relations Contact
Luis Amaral
View this press release online at: http://rwire.com/779605


text:
Your sound advantage
Games aren't just about graphics. Sound completes the picture. These made-for-gaming headphones deliver high quality stereo sound to keep you immersed in the action from start to finish. And the lightweight design feels so comfortable, you might even forget you're wearing a headset.
Game-quality stereo sound
- Powerful 40mm neodymium drivers deliver clear, rich sound
- Detailed soundstage
- Made for gaming: A pin drop, a bomb blast—hear it all
Folding, noise-cancelling mic
- Reduces distracting background noise
- Easy to rotate to best position for clearest voice pick up—or out of the way when you are not talking
Performance-sport cloth earcup pads
- Soft earcup covers designed to keep your ears comfortable, even after hours of gaming
- Breathable performance-sport cloth helps reduce heat and moisture build up during intense sessions
- Durable, washable covers slip off for easy cleaning
On-cable sound controls
- Easy-to-use volume dial and mute switch
- Keeps controls close at hand for quick and precise sound adjustments
- Extra-long cable lets you position controls right where you want them for quick access
Lightweight design
- Slim and light for maximum combat comfort
- A lighter, balanced assembly gives you a natural and stable fit
Flexible swivel earcups
- Adjust up to 90 degrees for a personalized fit that sits flush on your ears
- Swivels flat to protect earcups when you're not wearing them
What's in the box?
- Headset
- User documentation
What you need:
- Windows, Mac, or Linux computer
- 3.5mm headset and speaker inputs
Gaming-Grade Specifications:
- Driver: 40mm
- Frequency response: 20Hz-20KHz
- Impedance: 32 Ohms
- Sensitivity: 90dB SPL/mW
- Pickup pattern: Cardioid (Unidirectional)
- Type: Pressure Gradient Electret Condenser
- Frequency response: 50-20KHz
- Sensitivity: -40dBV/Pa re: 0dB = 1 Pa, 1KHz
- Test conditions: 3.0V, 2.2K Ohm


text:
Join Andrew and Jenny as they share Schneider Electric's journey to transform their Employer Brand strategy, including the re-launch of their People Vision and refreshed Employer Value Proposition for prospective candidates. With the transformed employer brand strategy, Schneider Electric has better aligned the way we hire to the way we work, ensuring our people goals work alongside our business strategy and vision. We want to ensure the way we recruit, work and do business also reflects our goal to be one of the most global of local companies in the world.
Schneider has reinvented their EVP to ensure an integrated and seamless approach throughout a company with over 135,000 people. Take away best-practice learnings on how to reinvent your EVP, including gaining leadership support, fostering collaboration with marketing and ideas to leverage existing company HR policies to their advantage.


text: It is not too late to book for our Christmas Quiz being held on Friday 30th November at St. James's Church Hall, Tower Road, Clacton on Sea, CO15 1LE. Teams of 4 to 6 people at £3.50 per head. Prizes for best Banquet. This quiz is raising funds to support the running of our Club. Follow the link for more details:


text:
So that's my life right now.
Watching Bakemonogatari (I'm late I know) and playing DFO.
Bakemonogatari is a portmanteau of bakemono (monster) and monogatari (tales), the translation as "Ghostory" is pretty clever I think.
I want to make a comparison to XXXHolic but it's not quite right there.
It is the same kind of eclectic material, except it's directed by Shinbo, so everything has an extra layer of WEIRD STYLE over them.
If you liked XXXHolic you might like this, it's like the same kind of material from a totally different point of view director-wise.
Also it's quite cute in a lot of areas, and a lot of the dialogue is really amusing.
DFO stands for Dungeon Fighter Online, or Arad Senki in Japan, or Dungeon and Fighter in Korean.
It's basically a free to play Streets of Rage the MMO, aka a 2D side scrolling brawler you play with up to three friends in a party.
The game is more fun than it has any right to be and made me realize that while 3d over the shoulder MMOs are pretty and I'll play them, I'll hardly stick with them because I get tired of the over the shoulder spam your skill nonsense.
And because it's built like a brawler/fighter IF YOU GET HIT it's cause you were dumb, not because you were forced to sit in front of a mob and spam skills at it and it spammed skills at you.
This game though has a really nice combo system, PVP is fun and silly as hell, I really like how the entire game works.
No blocking though, unless you're a slayer :V
Unfortunately Nexon USA is trying it's hardest to make me quit this game, first by raising the experience curve significantly compared to the asian versions, second by capping us at level 40 when level 60 is the normal max.
There's also a fatigue system that limits the amount of dungeons you can enter per day (PVP uses no fatigue), so raising the exp means basically artificially expanding the time it takes to get to max.
From what I have been told they actually have the newest client, and each patch is basically OVERWRITING the triggers that lock out the newest contet.
What a backwards way of rolling out a game.
And don't get me started on the item shop, you pay for avatar "tokens" and spend one to get a random item of a random type (so say a headpiece) with random stats.
With something like 5 looks and 4 stats that's $2.50 (holy crap) for a 1/20 chance of getting what you want.
Right now it would be actually more efficient to buy gold from all the Chinese gold spammers in town and buy your avatar items using in-game gold.
But anyway I hit level 40 (the last 3 levels are sheer torture ugh ironically because there is a very efficient two fatigue dungeon that takes six to ten minutes to complete, however since you start with 156 fatigue in terms of hours you're looking at six hours plus of grinding this dungeon because everything else gives less exp per fatigue point) so right now I'm just chilling.
If you can't tell the game has attracted quite a few "real" fighting game players.
Friday, October 16, 2009
3 comments:
I've actually been playing this too lately- casually, and I'm not sure how long I'll stick with it, but walking home at lunch and spending half an hour clubbing goblins with a giant totem pole has worked wonders for my morale in the office.
How long is it solo-able for? I'm at level 8 as a Priest and I haven't had any problems yet. Am I going to hit a wall at some point here?
- Akuun said...
It looks neat from your videos, but screw Nexon and the crap they include to deliberately inflict pain on their players.
- Blogger said...
eToro is the #1 forex trading platform for beginner and professional traders.
Subscribe to: Post Comments (Atom)


text:
On Howard Stern's SiriusXM show this week, Steven Tyler took his god-given share of the credit for Guns N' Roses long-awaited real reunion this year. Recalling a chance meeting sometime between one and three years ago, Tyler informed Stern, "I said, 'You need to get the fuck back together again soon, because we all miss you.' Same thing someone said to me when Joe [Perry, Aerosmith guitarist] and I were fighting."
And GN'R is super thankful—that they have the chance to burn the 67-year-old rocker through the magic of social media:
Blabbermouth notes that comic Andrew Dice Clay also mentioned having sway over Slash and Axl Rose. While talking to Stern, Tyler also said:
"There's a place and time, where if you don't [reunite], you're gonna miss it. And in another four or five years, no one's even gonna know who they were or care if they were on tour. ... I have such a huge ego about keeping the fire and the music together, because if people come up to me and say, 'Dude, I got laid to your music,' or a woman comes up and says, 'We got married to your songs'—you had something to do with the fabric of people. Guns N' Roses had something to do with the fabric of humans on this planet."
And the man stitching that fabric, from his big hammock in the skies of rock and roll deities, was Steven "Sweet Emotion" Tyler. JK buddy—but seriously, ya burnt.