text:
Roseville Boudoir Photography | She's Doing it Again
What was your biggest fear before booking your Roseville boudoir photography session? Did it come true, and if not, what happened instead?
My biggest fear was showing all the imperfections and flaws through the camera, but it now how it all turned out. The finished photos were AMAZING!
What, specifically, was your favorite part of your Roseville boudoir photography experience, and why?
The entire experience was very liberating. It allows you to look deep within yourself and trust that you are amazing just as you are.
The experience as a whole was great from shopping, figuring out what I want to do and wear, and then coming into the studio and bringing it all to life.
I hope to look at these photos one day when I'm 80 years old and think WOW that was me.
But actually I received that gift sooner than I expected, because I look at them now and think that. In fact, I am now doing my second session and look forward to doing something different.
It's a magical experience!!
What advice would you give to someone considering booking their Roseville boudoir photography session with us?
Don't wait. Do it now. The longer you wait, the more you will regret that you didn't do it sooner. It's a personal gift to one self. It's a magical experience!!
What responses did you get from your Beloved/friends/family about your images?
The response was unbelievable, everyone I showed the photos to LOVED them.
What types of negative things (if any) did you tell yourself before your Roseville boudoir photography session?
I'm almost 50 and worried about every last flaw. I had a c-section and my stomach is not photograph-able.
What did your experience do to silence those doubts?
The photographer helped silence those doubts. Jillian made me feel so comfortable with the entire experience, including myself.
What did you take away from your Roseville boudoir photography experience that surprised you the most?
I was surprised how much I loved the process. It's almost like a high or rush.
If you could give your Roseville boudoir photography session a title, what would it be?
Do It Again!!


text:
I'll show you how different we are now
I'll show you how different we are now
-Matsuoka Rin-
This is my entry for the red challenge. I hope it's red enough! I kind of failed with brush work in my last piece, so I went back to my specialty which is using textures ahaha. This was originally going to be a gif, but I liked it fine as a graphic. I hope you guys like it too!

subset: commoncrawl


text:
You'd think after being delayed on the ground for six hours anyone, and especially a toddler, would be well and truly over being stuck on a plane.
That wasn't the case for Phoenix Preston, a four year old who was delayed on her first ever flight.
Preston was travelling from Chattanooga, Tennessee, to Orlando, Florida, to visit her father but was delayed due to a mechanical fault.
Despite many on board being aggrieved by the delay, Pheonix's mother, Leandra, said her daughter's excitement never wavered - and it wasn't long before her unbridled wonder and optimism enthralled her fellow passengers, too.
Capturing the moment the plane finally begins to taxi and Phoenix, like her namesake, begins to rise - the tot adorably exclaims, "this is awesome. Yee-haw," as other passengers can be heard laughing in the background.


text:
Professional Services
CLA Personnel works closely with multiple professionals in order to provide expert services in various fields: access to information and privacy, procurement, project management, finance, communications, human resources and more.
In order to facilitate the hiring process of our consultants and bring help onboard fast, CLA Personnel is qualified on the following standing offers and supply arrangements:
Temporary Help Services, Stream 5 professional services
Task and Solutions Professional Services (TSPS)
Need professional services fast? Contact us today to discuss the procurement method that best suits your needs or ask your questions with our contact form


text:
Back to Journals » Drug Design, Development and Therapy » Volume 11
In vivo study of doxorubicin-loaded cell-penetrating peptide-modified pH-sensitive liposomes: biocompatibility, bio-distribution, and pharmacodynamics in BALB/c nude mice bearing human breast tumors
Authors Ding Y, Cui W, Sun D, Wang GL, Hei Y, Meng S, Chen JH, Xie Y, Wang ZQ
Received 23 August 2017
Accepted for publication 4 October 2017
Published 26 October 2017 Volume 2017:11 Pages 3105—3117
DOI https://doi.org/10.2147/DDDT.S149814
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Anastasios Lymperopoulos
Yuan Ding,1,* Wei Cui,2,* Dan Sun,1 Gui-Ling Wang,1 Yu Hei,1 Shuai Meng,1 Jian-Hua Chen,3 Ying Xie,1 Zhi-Qiang Wang4
1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, 2School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 3School of Medicine, Jianghan University, Wuhan, People's Republic of China; 4Department of Chemistry and Biochemistry, Kent State University Geauga, Burton, OH, USA
*These authors contributed equally to this work
Abstract: In vivo evaluation of drug delivery vectors is essential for clinical translation. In BALB/c nude mice bearing human breast cancer tumors, we investigated the biocompatibility, pharmacokinetics, and pharmacodynamics of doxorubicin (DOX)-loaded novel cell-penetrating peptide (CPP)-modified pH-sensitive liposomes (CPPL) (referred to as CPPL(DOX)) with an optimal CPP density of 4%. In CPPL, a polyethylene glycol (PEG) derivative formed by conjugating PEG with stearate via acid-degradable hydrazone bond (PEG2000-Hz-stearate) was inserted into the surface of liposomes, and CPP was directly attached to liposome surfaces via coupling with stearate to simultaneously achieve long circulation time in blood and improve the selectivity and efficacy of CPP for tumor targeting. Compared to PEGylated liposomes, CPPL enhanced DOX accumulation in tumors up to 1.9-fold (p<0.01) and resulted in more cell apoptosis as a result of DNA disruption as well as a relatively lower tumor growth ratio (T/C%). Histological examination did not show any signs of necrosis or inflammation in normal tissues, but large cell dissolving areas were found in tumors following the treatment of animals with CPPL(DOX). Our findings provide important and detailed information regarding the distribution of CPPL(DOX) in vivo and reveal their abilities of tumor penetration and potential for the treatment of breast cancer.
Keywords: tumor targeting, TUNEL stain, hemolysis, therapy for breast cancer, pharmacokinetics
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]


text:
Enter your Email Address and click 'Continue' Example: email@example.com Submit Cancel Forgot Password Close /GarnetHill/US/Global/Popups/forgot_password Enter your email address
10 0
We apologize, but this item is no longer available.
false pdp {"scheme":"product1_cr"}
false pdp {"scheme":"product2_cr"}
Johnny Was Fletcher Tunic is rated out of 5 by 4.
Rated 5 out of 5 by Outdoor lover from Pretty Tunic It's a very pretty Tunic. True to the size. I like it can be mashing wash. Very happy to the purchase.
Date published: 2019-11-06
Rated 5 out of 5 by KKto from Great craftsmanship. True to size and flattering. I bought this for travel this winter for warmer climes and can't wait to hit the road. It's light, comfortable and gorgeous!
Date published: 2019-10-09
Rated 5 out of 5 by Lindabbb from Beautiful Top Love, love, love this top. I receive so many compliments every time I wear it.
Date published: 2019-09-09
Rated 4 out of 5 by JEH Texas from Pretty top Very nice top but runs large. Get a smaller size. Bit overpriced for value.
Date published: 2019-10-20
Is this more of a blue color or a gray??
This is more of a blue color. It does have gray undertones.
Date published: 2019-08-04
Product:47783-Johnny Was Fletcher Tunic


text:
Jay Som - Everybody Works [180g Orange Vinyl]
On her first proper album as Jay Som, Melina Duterte, 22, solidifies her rep as a self-made force of sonic splendor and emotional might. If last year's aptly named Turn Into compilation showcased a fuzz-loving artist in flux—chronicling her mission to master bedroom recording—then the rising Oakland star's latest, Everybody Works, is the LP equivalent of mission accomplished.
- Lipstick Stains
- The Bus Song
- Remain
- 1 Billion Dogs
- One More Time, Please
- Baybee
- (BedHead)
- Take It
- Everybody Works
- For Light


text:
How do I join?
1. Login to your account
Enter your email and password used
when registering for one of the
events we partner with.
2. Choose an event
Select a StudyAd
partner event.
3. Use your unique link
This unique link must be
used in all of your ads.
4. Share with your peers
Promote the events you have
selected among all of your contacts
via social media, email, SMS etc.
5. Check your progress
Check your
performance LIVE
6. Run for prizes
Run for $2,000USD
in tuition fees.
How does StudyAd work?
Developed by the world's largest study abroad events organizer, StudyAd is a contest model platform that allows students to compete for prizes such as financial aid and free courses by simply promoting study abroad events to their peers.
How can I log in?
In order to log in, please use the same e-mail and password you created when registering to any of the study abroad partner events (EDUEXPOS, THE STUDENT WORLD, EDUCANADA...).
How can I get another password?
In case you don't remember your password, please select the "forgot your password" option. The system will automatically send you a new one by e-mail.
Do I need to register to attend any of the partner events to be able to join StudyAd?
No, it's not mandatory to be registered but we highly recommend that you do. By registering for an event, you will not only have access to the event but you will also be notified about all of the benefits and details making it easier to promote.
Can I promote more than one event at the same time?
Yes, you can. Depending on your region you may only see one option.
How does the system track my progress?
When you join a campaign, the system automatically creates a unique link for your promotion. Only the people that register to attend the event through this link can be qualified and the system tracks each of your registrations.
When is the winner announced?
The announcing date is specified on the prize's page. StudyAd will communicate the winner by e-mail and on facebook.com/edufindme between 10 to 15 days after the event. In case of physical events, it will be communicated between 10 to 15 days after the last event of the tour.
What does each badge mean?
The badges only illustrate your progress. The more people register through your link and join the event the more entries you receive and therefore more badges you unlock.


text:
Another stunning photograph of dear Lily, whom we will say au revoir to later today. Though she's gone far too soon, she's left behind a legacy of children and grandchildren, a joie de vivre unlike any other, and a love story that spanned fifty years and several continents. Every year of my life was in some way marked by her presence and friendship; this is a tough one to wrap my mind around but again, it is the survivors whose grief is my priority, not my own.
In the words of The Little Prince, Il est très simple: on ne voit bien qu'avec le cœur. L'essentiel est invisible pour les yeux.
[Rough] translation: It is quite simple: one only sees well with the heart. What's essential is invisible to the eyes.
Today will be difficult to be sure, but we'll get through it. Merci pour tout, mon coeur.


text:
- Register
- Login
- The Arts
- Talents
- Crew
- Institutions
- Festivals
- Videos
No products in the cart.
Steve Ochogo is a passionate,seasonal and professional Kenyan actor who has been in the theatre(stage acting) and film Industry for ten years. He is the producer and Director of Galaxy Arts Entertainment. Apart from being an an actor he is also a script writer and a gospel musician. He has worked with productions such as Theatrix Arts, Jicho Four production, Phoenix Players, Heartstrings Entertainment, KBC(Plot Ten),Assignment Studios,Naswa( Citizen TV) as the Production Manager and head of Creative.
Kalasha International Awards.
(1)Best Original Screenplay ~Embraced
(2)Best Short Film ~Embraced
(3)Best supporting Actor~Embraced


text:
Human defensins are natural peptide antibiotics. On the basis of the position and bonding of six conserved cysteine residues, they are divided into two families, designated α- and β-defensins. Human α-defensins are expressed predominantly in neutrophils (human neutrophil peptides (HNP) 1–4) or intestinal Paneth cells (human defensins (HD) 5 and 6). Although α-defensins have been implicated in the pathogenesis of inflammatory bowel disease, their immunomodulatory functions are poorly understood. In the present study, HNP-1, HNP-3, and HD5 were found to be potent chemotaxins for macrophages but not dendritic cells using Gαi proteins and MAPK as signal transducers. α-Defensins were also chemoattractive for the human mast cell line HMC-1 but lacked, in contrast to β-defensins, the ability to induce intracellular calcium fluxes. Furthermore, HNP-1, HNP-3, and HD5 comparably mobilized naive as well as memory T lymphocytes. Using the protein kinase C (PKC) inhibitors GF109 and Gö6976, we observed a PKC-independent functional desensitization to occur between human α-defensins, which suggests a common receptor for HNP-1, HNP-3, and HD5 on immune cells. This α-defensin receptor was subject to heterologous desensitization by the PKC activator PMA and to PKC-dependent cross-desensitization by human β-defensins. Conversely, α-defensins desensitized β-defensin-mediated migration of immune cells in a PKC-dependent manner, suggesting unique receptors for both defensin families. Taken together, our observations indicate that chemoattraction of macrophages, T lymphocytes, and mast cells represents an immunomodulatory function which is evolutionarily conserved within the human α-defensin family and tightly regulated by β-defensins.
Human defensins are short cationic β-sheet peptides with molecular masses ranging from 3 to 5 kDa. They are divided into two families, α and β, based on the differences in the positions and three disulfide bonds of their six cysteine residues (1). α- and β-defensins are products of distinct gene families that are thought to have evolved from an ancestral β-defensin gene (2). Despite only modest amino acid sequence identity among α-defensins, a striking similarity on the level of tertiary structures was found (3, 4).
α-Defensins are produced primarily by neutrophils (human neutrophil peptides (HNP))5 or intestinal Paneth cells (human defensins (HD)) (5). The best known property of defensins is their ability to kill microbial pathogens. Their spectrum of activity includes bacteria, fungi, and viruses (6). An important role of α-defensins in the maintenance of intact mucosal barriers is suggested by the finding that mucosal peptide antibiotics are involved in the pathogenesis of inflammatory bowel disease (7). Notably, a deficiency of HD5 in Paneth cells is associated with Crohn′s disease of the ileum (8).
Recently, α-defensins have been shown to play significant roles in adaptive as well as innate immunity. They modulate the cytokine response of monocytes and lymphocytes (9, 10) and promote systemic Ag- or tumor-specific immune responses (9, 11, 12). In keeping with these findings, HNP-1 and -2 have been found to mobilize immature dendritic cells (DC) and naive but not memory T lymphocytes via as yet unidentified receptors (13). However, controversial results exist with regard to HNP-1/-2-induced migration of monocytes (13, 14, 15), whereas HNP-3 and HD5 are supposed to completely lack chemotactic activity (4, 14, 15). Clearly, studies of the immunomodulatory properties of α-defensins are still in their infancy despite the well-appreciated importance of these peptides for the host defense (16).
The purpose of the present study was to investigate the impact of human α-defensins on cells of the innate and adaptive immune system to 1) identify those immune cells that are primary targets for α-defensins, 2) define receptor usage by α-defensins, and 3) uncover cross-regulation between α- and β-defensins.
Materials and Methods
Reagents and cell lines
Pertussis toxin, MAPK ERK inhibitor PD098059, JNK inhibitor SP600125, p38 inhibitor SB203580, the general protein kinase C (PKC) inhibitor GF109, Gö6976, which inhibits calcium-dependent PKC isotypes, and PMA were all purchased from Calbiochem. Recombinant (human or murine) CCL3, CCL19, and CCL20 were obtained from PeproTech. Recombinant human anaphylatoxin C5a was described elsewhere (17). Commercial sources for HNP-1 were The Peptide Institute, Bachem, and PeproTech; HNP-3, HD-5, and human β-defensin (HBD) 2 were obtained from The Peptide Institute.
Human macrophages, DC, and mast cells
Leukocytes were obtained by leukapheresis from volunteer blood donors in the Department of Transfusion Medicine, University Clinic Göttingen (Göttingen, Germany). PBMC were isolated as described previously (17) and cultured for 1 h at 1 × 107 cells/ml in endotoxin-free RPMI 1640 (Biochrom) supplemented with 5% heat-inactivated autologous serum in flat-bottom plates. After washing off nonadherent cells, adherent mononuclear cells (>90% CD14+ monocytes) were used to generate macrophages or DC. DC were cultured in RPMI 1640 supplemented with 10% FCS (PAN Biotech), 2 mM l17).
To obtain macrophages, adherent PBMC were cultured in RPMI 1640 supplemented with 5% FCS, 2 mM l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 100 U/ml GM-CSF. After 1–3 days, cultured macrophages expressed low to moderate levels of HLA-DR but high levels of CD14.
The human mast cell line HMC-1 was cultured as described elsewhere (18).
Human lymphocytes
Human peripheral blood CD3+CD4+CD45RA+ naive or CD3+CD4+CD45RO+ memory T cells were purified from PBMC (see above) by magnetic cell sorting using a negative selection technique according to the manufacturer's recommendations (Miltenyi Biotec). The purity of T cell subsets was verified by FACS analysis and was always >95%.
Murine DC and macrophages
Generation of murine immature DC derived from BALB/c bone marrow precursor cells has been described previously (17). Nonadherent bone marrow cells were cultured in RPMI 1640 containing 5% FCS, 0.1 mM nonessential amino acids, 2 mM l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μM 2-ME, along with 100 U/ml murine GM-CSF (PeproTech) for a total of 6–7 days.
Murine bone marrow-derived macrophages were generated by culturing nonadherent bone marrow cells from BALB/c mice in RPMI 1640 containing 5% FCS, 0.1 mM nonessential amino acids, 2 mM l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μM 2-ME, and murine M-CSF (20 ng/ml; PeproTech) for 6–7 days. Adherent cells were >95% macrophages as judged by morphology and homogenous surface expression of C5a and C3a receptors and absence of CD11c.
The murine macrophage cell line J774A.1 was cultured as described elsewhere (19).
In vitro chemotaxis
In vitro chemotaxis was assayed using the HTS Transwell-24 system from Corning. Cells diluted at 1 × 106/ml in migration buffer (RPMI 1640 with 1% BSA) were placed in the upper wells, whereas the various chemoattractants diluted in migration buffer as indicated were added to the lower wells. For chemotaxis of human and murine macrophages and DC, polycarbonate membranes with a pore size of 8 μm were used and incubation was performed at 37°C in a 5% CO2 atmosphere for 90 min; if human T lymphocytes were used, pore size was 5 μm and incubation time was 4 h and for HMC-1 cells pore size was 8 μm and incubation was 3 h. Migration was stopped and migrated cells were detached by placing Transwell chambers for 15 min on ice. Thereafter, migrated cells in the lower chambers were counted using a hemocytometer. All determinations were performed in duplicate.
To investigate the role of PKC in receptor desensitization, cells (1 × 106 in 1 ml of medium) were preincubated in the presence or absence of the PKC inhibitors GF109 (4 μM) or Gö6976 (2 μM) at 37°C for 30 min and centrifuged.
For desensitization studies, cells (1 × 106 in 1 ml of medium) were preincubated at 37°C for 30 min with selected chemotaxins (200 ng/ml) or PMA (2 μM) and centrifuged.
To study the role of Gαi in signal transduction, cells were incubated for 2 h in the presence of different concentrations of pertussis toxin (Calbiochem) before their use in chemotaxis experiments. Treatment with pertussis toxin did not affect cell viability, as assessed by trypan blue exclusion.
To study the role of MAPK in signal transduction, cells were preincubated at 37°C for 30 min with PD098059, SP600125, or SB203580 (20 μM each) and centrifuged. Treatment with MAPK inhibitors did not affect cell viability, as assessed by trypan blue exclusion.
Migration of human cells in a SCID mouse model
All animal work was conducted in accordance with guidelines for animal welfare and was approved by the government of Lower Saxony, Germany. In vivo migration studies were performed as described elsewhere (17). SCID mice (strain CB-17 SCID of both sexes; weight 19–24 g) were obtained from Charles River Breeding Laboratories. Cells (1 × 107 in 200 μl of PBS) were injected into the tail vein of SCID mice along with a chemotaxin (10 or 20 μg in 200 μl of PBS) that was injected into the peritoneal cavity. After 4 or 15–18 h, mice were sacrificed and peritoneal cells were harvested by lavage. Subsequently, cells were stained with anti-HLA-DR-PE in combination with FITC-anti-CD14 or FITC-anti-CD86 to identify migrated human macrophages and DC, respectively. Absolute numbers of migrated human cells were calculated from the percentage of HLA-DR+ cells as determined by FACS analysis and the total peritoneal cell count.
For desensitization studies, human cells (1 × 107 in 1 ml of PBS) were preincubated at 37°C for 1 h with selected chemotaxins (2 μg), washed twice in PBS, and resuspended in 200 μl of PBS for injection. Pretreatment of cells did not affect cell viability, as assessed by trypan blue exclusion.
Migration of murine cells in vivo
In vivo migration studies were performed as described elsewhere (17). Murine bone marrow-derived cells were labeled with the red fluorescent dye PKH-26 (Sigma-Aldrich) according to the manufacturer's instructions. Cells (1 × 107 in 200 μl of PBS) were injected into the tail vein of syngeneic BALB/c mice (weight 20–24 g; age 8–20 wk) along with a chemotaxin (10 or 20 μg in 200 μl, as indicated) that was injected into the peritoneal cavity. Approximately 15–18 h later, mice were sacrificed and peritoneal lavage performed. Subsequently, peritoneal cells were counted and analyzed by FACS. Absolute numbers of migrated labeled cells were calculated from the percentage of red fluorescent cells and the total peritoneal cell count.
For desensitization studies, labeled cells (1 × 107 in 1 ml of PBS) were preincubated at 37°C for 1 h with selected chemotaxins (2 μg), washed twice in PBS, and resuspended in 200 μl of PBS for injection.
Calcium measurements
A total of 106 cells was loaded in 700 μl of RPMI containing 5% FCS, 1 μM Fluo3-AM, and 0.02% Pluronic F127 (both from Molecular Probes). Subsequently, the cell suspension was diluted 2-fold with RPMI 1640/10% FCS and was incubated for 10 min at 37°C. Cells were washed twice with Krebs-Ringer solution composed of 10 mM HEPES (pH 7.0), 140 mM NaCl, 4 mM KCl, 1 mM MgCl2, 1 mM CaCl2, and 10 mM glucose. The changes in fluorescence intensity of Fluo3 were monitored on a LSRII cytometer (BD Biosciences). Equal loading of the samples was controlled by treatment with 100 nM ionomycin (Sigma-Aldrich).
Statistical analysis
For the calculation of p values, the paired Student's t test was used. Differences with p < 0.05 were considered to be significant.
Mobilization of macrophages
We observed human α-defensins HNP-1, HNP-3, and HD-5 to induce concentration-dependent, bell-shaped chemotactic responses in human monocyte-derived macrophages in vitro (Fig. 1⇓A).
To address the issue of signaling via one or more chemotactic receptors, functional desensitization between human α-defensins was investigated. Preincubation of macrophages with HNP-1 or HD5 is shown to inhibit subsequent migratory responses to HD5 (Fig. 1⇑B). Although desensitization can be of homologous or heterologous nature, only the latter is PKC-dependent (20, 21). We observed that the general PKC inhibitor GF109 was unable to block desensitization between HNP-1 and HD5 (Fig. 1⇑B). This finding indicates that α-defensins signal through a common receptor in macrophages resulting in PKC-independent homologous receptor desensitization. Receptors for α-defensins were subject to heterologous desensitization as shown by the ability of the PKC activator PMA to desensitize HD5-induced chemotaxis (Fig. 1⇑B). Human macrophages have been found to migrate in response to different human β-defensins (22). We observed that PKC was instrumental in the cross-desensitization between α- and β-defensins because PKC blockade by GF109 abrogated the desensitizing effects of HNP-1 and HD5 on HBD-2-induced migration of macrophages (Fig. 1⇑C). Desensitization by α-defensins did not extend to other chemotactic receptors as shown in the case of C5a (Fig. 1⇑D) or CCL3 (data not shown).
α-Defensin-mediated migration of human macrophages was further studied using a SCID mouse model. We have recently shown that accumulation of human macrophages in the peritoneal cavity of SCID mice as a result of chemotactic cell migration was inducible by injection of anaphylatoxins and chemokines (17). We now observed that human macrophages could be mobilized into the peritoneal cavity of SCID mice by i.p. injections of α-defensins (Fig. 1⇑, E and F). Similar to in vitro chemotaxis experiments, in vivo migration of macrophages was abrogated if α-defensins were used for both preincubation and chemoattraction. Cross-desensitization between both defensin families was also observed in vivo (Fig. 1⇑, E–G). No inhibitory effect by α-defensin preincubation was observed when C5a was used as a chemotaxin in vivo (Fig. 1⇑H). C5a was also unable to desensitize HNP-1-induced chemotaxis (Fig. 1⇑E). These experiments confirm the in vivo relevance of α-defensin-induced chemoattraction and desensitization.
Mobilization of DC
IL-4 which is commonly used for the generation of DC in vitro was recently observed to inhibit migration of monocyte-derived macrophages and DC in response to anaphylatoxins (17). In line with these findings, IL-4 treatment also inhibited migration of human monocyte-derived DC in response to HNP-1 in vitro and in vivo (Fig. 2⇓A). Thus, macrophages but not DC appear to constitute primary target cells for α-defensins. To find further support for this hypothesis, we investigated α-defensin-induced migration of murine macrophages and DC which had been generated from bone marrow precursors in the presence of M-CSF and GM-CSF, respectively (Fig. 2⇓B). It was found that HNP-1 preferentially mobilized macrophages but not DC in vitro and in vivo. Of note, murine DC retained the ability to migrate as shown by their potent chemotactic response to CCL20 (Fig. 2⇓B). In support of its role as a chemoattractant for macrophages, HNP-1 recruited the murine macrophage cell line J774A.1 (Fig. 2⇓C).
Mobilization of T lymphocytes
HNP-1 and HD5 were observed to be potent chemotaxins for both naive as well as memory T lymphocytes (Fig. 3⇓, A and B). Of note, we also identified HNP-3 to recruit memory T cells (Fig. 3⇓B).
In analogy to human macrophages, the general PKC inhibitor GF109 was unable to block functional desensitization between human α-defensins (Fig. 3⇑C). This finding suggests that HNP-1, HNP-3, and HD5 signal through a common receptor also in T lymphocytes, resulting in PKC-independent homologous receptor desensitization. The α-defensin receptor on T cells was subject to heterologous desensitization following PKC stimulation by PMA (Fig. 3⇑C).
Mobilization of mast cells
The cell line HMC-1 is widely used as a model for immature human mast cells. We observed human α-defensins HNP-1, HNP-3, and HD-5 to induce concentration-dependent, bell-shaped chemotactic responses in HMC-1 cells (Fig. 4⇓A).
In analogy to human macrophages and T cells, the general PKC inhibitor GF109 was unable to block the functional desensitization between HNP-1 and HD5 (Fig. 4⇑B). Thus, human α-defensins signal through a common receptor also in mast cells. Mast cells have been shown to migrate in response to HBD-2 (23). We observed that the α-defensin receptor on HMC-1 mast cells was subject to heterologous desensitization by the PKC activator PMA and to PKC-dependent cross-desensitization by HBD-2 (Fig. 4⇑B). Conversely, α-defensins desensitized HBD-2-mediated migration of mast cells in a PKC-dependent manner, suggesting unique receptors for both defensin families (Fig. 4⇑C). Usage of the PKC inhibitor Gö6976 allowed us to investigate the involvement of calcium-dependent PKC isotypes (Fig. 4⇑, D and E). Gö6976 abrogated the desensitizing impact of HBD-2 on HNP-1-induced migration but, vice versa, failed to inhibit the desensitizing influence of HNP-1 on HBD-2. Thus, HBD-2 but not HNP-1 or HD5 signal through calcium-dependent PKC isotypes, a finding which is consistent with the inability of HNP-1 or HD5 to induce calcium fluxes in HMC-1 in contrast to HBD-2 (Fig. 4⇑, F and G).
Involvement of MAPK and Gαi
To determine the role of MAPK in α-defensin-induced signaling pathways, we used a pharmacological approach. Human macrophages (Fig. 5⇓A) were pretreated with PD098059, SP600125, or SB203580, which inhibit MAPK ERK, JNK, and p38 phosphorylation, respectively. We found that predominantly ERK and p38 contributed to α-defensin-induced chemotaxis.
Furthermore, pretreatment of macrophages (Fig. 5⇑B) and HMC-1 mast cells (Fig. 5⇑C) with pertussis toxin for 2 h inhibited HNP-1-induced chemotaxis in a dose-dependent manner. The 50% inhibitory concentration of pertussis toxin was ∼50 ng/ml in macrophages and 12.5 ng/ml in HMC-1 cells. These data indicate that Gαi proteins are instrumental in α-defensin-induced signal transduction.
Defensins function as important effectors of innate immunity. Their main function appears to be the killing or inactivation of microorganisms (24). Recent evidence suggests that defensins may be involved in adaptive immunity by attracting immunocompetent cells to the sites of inflammation and infection (22).
Investigating chemotaxis in vitro and in vivo, we found that HNP-1, HNP-3, and HD5 are chemoattractive for human monocyte-derived macrophages. HNP-1 and -2 have been shown in the past to recruit monocytes (14) or to be devoid of this activity (13, 15). HNP-3, in contrast, was described as chemotactically inactive, a finding which was surprising because HNP-1 to -3 differ at only the N-terminal amino acid (25). The intestinal α-defensin HD5 shows only moderate sequence similarity to neutrophil α-defensins (26) and was also observed to lack chemotactic activity (4). According to our data, however, recruitment of human macrophages is a function common to neutrophil as well as intestinal α-defensins including HNP-1, -3, and HD5. α-Defensin-induced migration was also detected using the murine macrophage cell line J774A.1 or murine macrophages differentiated from bone marrow precursors in the presence of M-CSF. Thus, mobilization of macrophages represents a common, evolutionarily conserved property of the human α-defensin family. The discrepancies between our data and those shown by others could originate from the different defensin preparations applied. We obtained synthetic HNP-3 from a commercial source whereas HNP-3 found to be chemotactically inactive was purified from neutrophils (14, 15). Other possible reasons may be the different methods for investigating in vitro chemotaxis (Transwell system vs microchemotaxis chambers). Furthermore, we used cultured macrophages which may express higher numbers of defensin receptors than freshly isolated monocytes.
DC have been demonstrated to migrate in response to gradients of HNP-1 via an unidentified receptor (13). According to our results, however, human and murine DC generated from monocytes and bone marrow precursor cells, respectively, were only marginally mobilized by α-defensins in vitro and no migration was observed using an in vivo migration assay. Therefore, we conclude that macrophages but not DC represent primary target cells for α-defensins. In keeping with this reasoning, IL-4 which is commonly used for the generation of human monocyte-derived DC was identified as a suppressor of α-defensin-induced chemotaxis. This finding is reminiscent of the inhibitory effect of IL-4 on anaphylatoxin-induced DC migration as a consequence of receptor down-regulation (17).
Chertov et al. (15) identified HNP-1 and HNP-2 as chemoattractants for CD3+ T cells whereas HNP-3 was inactive. Later, Yang et al. (13) reported that naive but not memory T cells migrated in response to HNP-1. In contrast, we found neutrophil HNP-1 and -3 to be comparably chemotactic for naive and memory T lymphocytes. Furthermore, our data prove that intestinal HD5 has the capacity to recruit naive and memory T cells, a finding which contrasts with a previous report on the chemotactic inactivity of intestinal α-defensins (4). It is tempting to speculate that the protective role of HD5 in host defense, specifically in the pathogenesis of ileal Crohn′s disease (7, 8), may in part result from its chemotactic effects on immune cells, including T lymphocytes and macrophages which may supplement or even exceed its antimicrobial activity. Indeed, the antimicrobial effects of many host defense peptides are sensitive to physiological salt concentrations in vitro which may favor the view that immunomodulation is the predominant function of defensins in vivo (27).
Rat peritoneal mast cells respond to human neutrophil α-defensins with histamine secretion (28). The underlying mechanism of action was found to be receptor-independent similar to other polycationic secretagogues such as substance P or polylysine. In contrast, the cell line HMC-1 which is widely used as a surrogate for human mast cells responded with chemotaxis to gradients of HNP-1, -3, and HD5 as shown in the present study. The chemotactic impact of α-defensins on mast cells is obviously receptor-mediated because chemoattraction was subject to homologous as well as heterologous desensitization.
Receptor desensitization is a regulatory mechanism to prevent the damaging effects of prolonged or excessive activation of G protein-coupled receptors (21). Heterologous or cross-desensitization involving different G protein-coupled receptors is commonly mediated by second messenger-regulated kinases such as PKC and does not require agonist occupancy (20). In contrast, homologous desensitization affects receptors in the agonist-occupied state and involves phosphorylation by G protein-coupled receptor kinases (21). We found that the functional desensitization between α-defensins occurred without participation of PKC, indicating that a single receptor is engaged. The receptor for α-defensins was also subject to heterologous desensitization because the PKC activator PMA abrogated defensin-induced chemotaxis.
Intriguingly, the ability of α-defensins to desensitize the chemotactic activity of β-defensins and vice versa was also PKC-dependent, indicating cross-desensitization to occur between distinct defensin receptors. In support of our findings, HBD-2 has been shown to signal through as yet unidentified G protein-coupled receptors on various immune cell subtypes such as macrophages and mast cells (22, 23). In HMC-1 mast cells, we made the observation that calcium-dependent PKC isotypes were involved in the HBD-2-mediated cross-desensitization of the α-defensin receptor which was consistent with the capability of HBD-2 to induce cellular calcium fluxes. Conversely, HNP-1 and HD5 did not engage calcium-dependent PKC isotypes when cross-desensitizing the HBD-2 receptor which was in line with their inability to induce calcium fluxes. It is noteworthy that calcium-independent PKC isotypes have been demonstrated to mediate cross-desensitization of chemokine receptors by opioid receptors (29). In contrast to their impact on defensin-induced cross-desensitization, PKC inhibitors were unable to block migration in response to α-defensins and HBD-2. These results are in line with published evidence that the monocyte chemotactic response to chemoattractants such as fMLP and chemokines is independent of PKC activation (30).
α-Defensin peptide sequences exhibit a large degree of sequence variability and divergent antimicrobial properties (1, 6). The molecular basis for the evolutionarily conserved binding of neutrophil as well as intestinal α-defensins to a common chemotactic receptor on immune cells may reside in the similarity of their tertiary structures which are stabilized through three conserved disulfide bridges (3, 4).
We found α-defensin receptors to be coupled to Gαi proteins due to their sensitivity to pertussis toxin, which confirms a previously published observation (13). G protein-coupled receptors are known to be connected to the MAPK signaling pathways by classical G protein-stimulated synthesis of second messengers (31). Mammalian cells contain three major classes of MAPK: ERK, JNK, and p38. All three kinases have been shown to play crucial roles in cell migration by distinct mechanisms (32). Our results confirm the importance of MAPK also for α-defensin-induced chemotaxis with p38 and ERK being predominantly required for HNP-1-induced migration of macrophages. In line with these data, p38 and ERK have been observed to participate in the degranulation of mast cells by HBD-3 and -4 (33).
In summary, human α-defensins are characterized by evolutionarily conserved receptor usage in macrophages, mast cells, and lymphocytes. The existence of a common receptor for α-defensins strengthens the view that immunomodulation may represent a principal function of the defensin system.
We thank Olga Walter and Kerstin Eckelmann for excellent technical assistance.
The authors have no financial conflict of interest.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
↵1 This work was supported by a grant from the Deutsche Forschungsgemeinschaft to A.S. (SO 478/1).
↵2 J.G. and A.S. contributed equally.
↵3 Current address: Merck KGaA, Darmstadt, Germany.
↵4 Address correspondence and reprint requests to Dr. Jörg Zwirner, Department of Cellular and Molecular Immunology, Georg-August-University Göttingen, Humboldtallee 34, Göttingen, Germany. E-mail address: jzwirne{at}gwdg.de
↵5 Abbreviations used in this paper: HNP, human neutrophil protein; HD, human defensin; DC, dendritic cell; PKC, protein kinase C; HBD, human β-defensin.
- Received March 29, 2007.
- Accepted July 6, 2007.
- Copyright © 2007 by The American Association of Immunologists


text:
Choice of game
I had laid out my plans for this match long before the tournament, believing this could have been Dravid's final game of competitive cricket. An overnight trip from Delhi to Jaipur felt like a pilgrimage to see the legend take the field.
Team supported
Rajasthan Royals. Dravid's presence, Fortress Jaipur and Otago's 14-in-a-row streak gave it a mini-Kolkata 2001 feeling.
Key performer
Despite Brad Hodge's match-winning, iceman-like innings, Rahul Shukla's revelatory bowling performance, James Neesham's all-round performance, my pick is Ajinkya Rahane. His innings set up the chase and held it together for the most part, and its technical perfection and class would have made his captain proud.
One thing I'd have changed
Shaun Tait bowling on this pitch in tandem with the rampant Shukla would have been a treat to watch. With a generous sprinkling of grass and quite some assistance on offer, Royals could have inflicted greater damage on Otago in the Powerplay. Instead, Tait was in the dugout, talking to commentators on air.
Face-off I relished
Pravin Tambe v Ryan ten Doeschate. Not the biggest names on the pitch, but these two dished out a duel that took us back to the days of classic, flighted legspin, with the odd flatter one thrown in. Having come in with his team tottering at 20 for 3, ten Doeschate showed no signs of trouble against any bowler, attacking with gusto. But when he tried stepping out repeatedly to Tambe, he was beaten by a combination of flight, pace and dip. In his third over, Tambe trapped ten Doeschate in front with a flatter one. A week shy of turning 42, Tambe has been doing everything in his capacity to prove Douglas Adams right.
Wow moment
Shane Watson's (dead) ball of the century, which had about as much chance of landing as there is of Watto not reviewing an lbw dismissal. Watson should have looked up to the commentary box for empathy. Who better than Murali Kartik, the last man to perform this wayward feat against a Kiwi team, to go to in such times?
The acrobat
Pravin Tambe's exploits on the field have thus far gone unnoticed. An acrobatic dive at the boundary, followed by poor recovery, cost Royals a boundary. However, Tambe would not give up trying. He dived again and saved overthrows for his team. It reminded us of a certain Bermudian policeman's efforts, six years ago.
Close encounter
For the first few overs, most of the spectators did not recognise the fielder at fine leg. But after the fourth over, things changed dramatically. Rahul Shukla became the more-rooted-for Rahul throughout the Otago innings, which is no mean feat considering his namesake's stature.
Before the game, Murali Kartik and Sanjay Bangar, who are part of the Hindi commentary team, were engaged in a conversation on the field. Suddenly Kartik started mock-punching and shadow boxing his old Railways team-mate .
Shot of the day
Neesham played quite a few exquisite shots, including a picture-perfect cover drive off Watson. However, the shot of the day came from Rahane's bat. For the better part of his innings, time seemed to stop as per his whims. A couple of cover drives off Neil Wagner left us wanting more.
Crowd meter
The turnout was sparse, probably because Rajasthan had already qualified. That is not to say that there was a dull moment in the game. At the beginning, the announcer called out to Rajasthan supporters, asking them to stand up. The whole stadium did so. As if to remind the world that all was well with our game, the crowd did the same when it was asked for Otago. Well, as they say, at the end of the day, cricket is the real winner.
Chant of the day
When Otago lost a flurry of early wickets, the crowd started cheering for them, wanting them to get to a competitive total to ensure a good game. Towards the end, every boundary was met with raucous cheering, every run was applauded. Ian Butler's boundaries were met with: "Jeetega bhai jeetega, Butler jeetega [Butler will win"].
ODI v Twenty20
I prefer ODIs, because they provide a lot more time for reflection and discussion. T20s leave us with very little time for anything apart from the dance-cheer-dance routine.
Marks out of ten
7. Tait's absence, Dravid's dry run and the lack of a nail-biting finish (yes, I'm looking at you and the tied Super Over, Otago), meant the game fell short of the perfect score. While the vantage point and the vacant seats in our stand ensured an excellent view of the proceedings, the lack of spectator-friendliness from volunteers was a significant negative. We ended up with a damaged credit card due to rash security checks, and were not allowed to stay back for the presentation ceremony, for reasons unexplained to us.


text:
The Garden Bridge Controversy
UPDATE - February 2016
Objection to proposed cycle stands in Queen Anne's Gate
We have written a formal objection to the siting of cycle stands in front of some of the best preserved and attractive early eighteenth centry houses in London.
Please see our letter in full on our Planning page. FM Conway Ltd is the service provider carrying out the consultation on behalf of Westminster City Council.
Thorney Tales (6) - St Margaret's Church, the amazing history of a window
Courtesy of The British Museum
The beautiful stained glass window which dominates the east side of St Margaret's Church can best be described as ill-timed. It was created in Holland around 1526 to celebrate the marriage of Henry VIII to Catherine of Aragon. But by the time it had been finished Henry had become besotted with Anne Boleyn. Henry is depicted in saintly contemplation in the panel at the bottom left of the window while on the right hand side of the window Catherine, against an almost identical background, is also deep in prayer. Both appear to be looking at the Crucifixion which occupies the whole of the central panel of the window.
Read more: Thorney Tales (6) - St Margaret's Church, the amazing history of a window
Annual Review 2014-15
Our AGM was held on Wednesday 18th November 2015 at the Grange Rochester Hotel.
After the official business of the meeting, Dr Caroline Shenton,
an archivist, historian and writer, gave a fascinating and
humerous talk about her book The Day Parliament Burned Down.
Please go to the About Us page to view the Minutes of our AGM and our Annual and Financial Reports.
A visit to the hidden archives of Westminster Abbey, 4th November 2015
The Thorney Island Society came into being as a result of saving London's first public library in Great Smith Street in 1985. So it was with a double reverence that we made our much anticipated visit to a far older library only a few hundred yards away in Westminster Abbey. Our objective was the Muniments Room where ancient documents, particularly about Abbey transactions, are stored. But to get to it you have to go through a library that Time has told to stand still. Matthew Payne, Keeper of the Muniments, had us enthralled as he explained the history all around us dominated by the overpowering hammerbeam oak roof dating back to 1450. Only three books survived the Dissolution of the Monasteries in the 1540s - probably because they were about coronations and not associated with the old religion - but the library has since acquired a book of 1477 and has a fragment of the History of Troy printed by William Caxton (c 1422 - 1491) at his press mere yards away from where we were standing.
A wooden spiral staircase took us up to the Muniments room itself where we enjoyed a spectacular view of the inside of the Abbey - at least when we could take our eyes off the ancient memories around us including a long oak chest dating back to 1159, million believed to be the oldest in the country. Among other treasures shown to us were the beautiful prayer book of Margaret Beaufort, mother of Henry V11, who died in 1509 and what seemed to be a document of Offa - yes, he of Offa's Dyke - dating back to 693 though it turned out to be a 12th century reproduction. However, the Abbey does have an authentic document that can be traced back to 959, which is long enough ago for most of us.
With that we bid our farewells to a remarkable part of the Abbey beyond the reach of most visitors. Our thanks to Matthew Payne for showing us around and to Pippa Parsons for organising the trip.
Paddington Tower
Thorney Tales (5) - Westminster Hall Roof
Visit to new Parliamentary Education Centre, 28th October 2015
Discovering Parliament through virtual reality
Construction of the new Education Centre for Parliament has proved controversial for some Thorney Island members. This was partly because it encroached on rare green space in Victoria Tower Gardens and partly because it was difficult to believe that a new building costing £7 million was going to be "temporary" for just 10 years as claimed.
At the end of October we visited it and were very impressed with the facilities provided to enlighten school children of primary and secondary age about the history of our parliamentary democracy. A series of rooms mimicing the look of the House of Commons and Lords utilise augmented reality techniques to explain over a thousand years of history at rapid speed to 100,000 of today's techno savvy kids every year.
Read more: Visit to new Parliamentary Education Centre, 28th October 2015
Thorney Tales (4) - The tomb behind one of Britain's biggest fortunes
St Margaret's churchyard is not short of famous dead bodies, being the resting place of
William Caxton, Sir Walter Raleigh, Wenceslaus Hollar, the brilliant engraver of London,
and the largest number of regicides you'll find anywhere. But there is only one tomb
above ground.
Close to the road between St Margaret's church and Parliament Square, it is regularly ignored by passers by but is the source of one of the wealthiest families the country has ever known.
Read more: Thorney Tales (4) - The tomb behind one of Britain's biggest fortunes
Visit to the RHS Lindley Library, 14th July 2015
- Westminster Coroner's Court
- 10 Greycoat Place - Developer's Consultation
- Visit to the RHS Lindley Library
- St. James's and The Green Park Update and Movement Strategy - 10th July 2020
- Visits & Events 2020-2021 - Details
- Caring for the Poor and Vulnerable on Thorney Island, Guided Walk
- Stephen Myers
- The Truth behind the National Holocaust Memorial in Victoria Tower Gardens
- Bearskins, Bayonets and Bravery - New Guards Museum podcast
- Victoria Tower Gardens - London Parks & Gardens Trust Fundraising for Judicial Review
- Two Africans with strong links to Thorney Island escaped slavery to become role models in London over 250 years ago
- Collecting fine bookbindings Zoom Talk - June 2020
- RUSI, The Royal United Services Institute, Whitehall
- London History Day, 31st May 2020 - 10 Old Pye Street
- Urban Tree Festival 2020 - Online
- Live Video of Feeding St James's Park Pelicans
- St Stephen's Church, Rochester Row
- Westminster Kingsway College
- Save Victoria Tower Gardens - Planning Inquiry Postponed
- Thorney Tales (19) Revd James Palmer and Palmer's Village
- Visits & Special 35th Anniversary Celebrations 2020
- Workshop by Jens Jakobsen, Master Florist at the Blewcoat School - March 2020
- Consultation Begins - Southside, Victoria Street
- Ignatius Sancho, Short Talk by Victor Keegan - February 2020
- Christchurch Gardens Reconstruction Short Talk - January 2020
- Christmas Party 2019
- 55 Broadway - Planning application
- Richmond House, Whitehall - The Northern Parliamentary Estate
- 20mph Throughout Westminster ? YES !
- The Society's 33rd AGM, 12th November 2019
- Visit to the Palace of Westminster & Jewel Tower - October 2019
- Thorney Tales (18) The Buxton Memorial Fountain
- Mission: Invertebrate, The Green Park - August 2019
- Tour of Buckingham Palace Gardens - August 2019
- From Beer to the Bard - A Victoria Walk by Anthony Davis - July 2019
- Special Tour of Westminster Abbey - July 2019
- Gems of Thorney Island, A Walk by Victor Keegan - July 2019
- The Guards Museum, Wellington Barracks - Volunteers
- Victoria Tower Gardens, poem by Elizabeth Witts
- The Green Park Tree Walk - May 2019
- Visit to Bonhams Auctioneers - May 2019
- Visit to Westminster Cathedral - May 2019
- Visit to The Speakers House - April 2019
- Ann Carlton our Co-founder
- Thorney Tales (17) Westminster Coke and Gas Company
- Duck Island Volunteer Project
- Illuminated River
- Visits & Events 2020-2021
- Dolphin Square Redevelopment
- Centenary of Armistice Cenotaph Service and Exhibition in St James's Park
- Visit to The Queen's Jubilee Galleries, Westminster Abbey - September 2018
- Thorney Tales (16) College Hall, Westminster Abbey / School
- Relocation of Emmeline Pankhurst statue from Victoria Tower Gardens
- Visit to The Church House - August 2018
- "The Devil's Acre before Peabody" Talk - August 2018
- Visits to Westminster School - July 2018
- Purcell Musical Tour of Westminster Abbey - June 2018
- Reception to mark the opening of The Queen's Jubilee Galleries, Westminster Abbey - June 2018
- Proposals for Christchurch Gardens, Victoria Street - June 2018
- Thorney Island Sources Tour of Westminster Archives - June 2018
- Visit to the Institution of Civil Engineers - May 2018
- St James's Park Tree Walk - May 2018
- Unveiling of Millicent Fawcett Statue in Parliament Square - April 2018
- Visit to Watts & Co, Ecclesiastical Furnishers & Outfitters - April 2018
- Broadway & Carteret Street
- Thorney Tales (15) Henry Purcell's Birthplace
- AGM 2017 & Talk
- Townsend House, Greycoat Place
- Thorney Tales (14) Siegfried Sassoon
- Chelsea College of Art & Design Collaboration
- Introducing our New Chair
- Lambeth Bridge Roundabout
- Tour of St James the Less church in Pimlico - September 2017
- Local Tree Walk with Paul Akers, WCC Arboriculturalist - September 2017
- Visit to HM Treasury building - July 2017
- Thorney Tales (13) - Elizabeth Woodville
- Suffragist Millicent Fawcett - First women honoured in Parliament Square ?
- Visit to St Margaret's Church - July 2017
- Parks: Our Shared Heritage Exhibition - July/August 2017
- Consultation by WCC on Future Growth/Building Height
- Proposed memorial statue to Lady Margaret Thatcher in Parliament Square
- Thorney Tales (12) The Jerusalem Chamber
- 61-71 Victoria Street
- Vauxhall Bridge Road/Tachbrook St Draft Development Opportunity
- Visit to Tate Britain's Archives & Library, November 2016
- Thorney Tales (11) - The Abbey Garden
- St Andrew's Club - 150th Anniversary, October 2016
- June Stubbs 1927 - 2016
- Henry V's Chantry Chapel Visit, October 2016
- Thorney Tales (10) - Oliver Cromwell v Charles I
- The Victoria Tower Gardens & The National Holocaust Memorial & Learning Centre
- Old War Office, 57 Whitehall
- Our visit to Parliament's hidden medieval places, 28th July 2016
- Westminster Fire Station
- Thorney Tales (9) - Governor of Duck Island
- Thorney Tales (8) Westminster Opera House
- Fundraising Gala Dinner September 2016
- Fundraising Gala Dinner September 2016
- New Scotland Yard, 10 Broadway
- What is Thorney Island ?
- Millbank Tower grows taller
- Welcome
- Thorney Tales (7) - Storey's Gate
- Thorney Tales (6) - St Margaret's Church, the amazing history of a window
- Paddington Tower
- WCC say "No" (AGAIN!) to proposed sculpture outside Westminster Central Hall
- Objection to proposed cycle stands in Queen Anne's Gate
- A visit to the hidden archives of Westminster Abbey, 4th November 2015
- Visit to new Parliamentary Education Centre, 28th October 2015
- Thorney Tales (5) - Westminster Hall Roof
- Thorney Tales (4) - The tomb behind one of Britain's biggest fortunes
- Thorney Tales (3) - The Jewel Tower
- Visit to the RHS Lindley Library, 14th July 2015
- Thorney Tales (2) - The River Tyburn
- The Garden Bridge Controversy
- Visit to Bridgewater House
- Queen's Walk Cycle Route, The Green Park
- Thorney Tales (1) - Britain's Hidden Treasure Trove
- AGM 2016 & the Panorama of The Thames Project
- Annual Review 2014-15


text:
The Cost of Living Adjustment (COLA) on our pension is based on the increase in the Consumer Price Index (CPI) over a 12 month period ending on October 31 of each year.
· 100% of the increase in the CPI (rounded to the nearest whole number), to a maximum of 2%
· 60% of the increase in the CPI (rounded to the nearest two decimal points), to a maximum of 4%.
On November 17, Statistics Canada released the CPI data for the last twelve months. According to Statistics Canada, the CPI rose 1.4% between October 2016 and October 2017 for Canada as a whole.
Based on this, the Cost of Living Adjustment on our Bell Canada pensions should be 1% in 2018. The increase will appear on the January pension payment.


text:
Wondering how to get FIFA 19 for free on xbox 360 console?
The long wait has come to an end, because now everyone
can enjoy FIFA 19 ISO without hacking Xbox 360. All one has to do is to download
FIFA 19 Xbox 360 free ISO by clicking the blinking button above and copy it to you console to play.
Inevitably it takes a little extra work to move FIFA 19 ISO on your console,
but hey, it's free and free is always worth it. But it's really handy actually. You can either use a external hard
drive and plug it up on your Xbox 360 or you can just burn the download ISO into DVD
and start playing FIFA 19 ISO. And no need to worry, all the required instructions on how to install and play
FIFA 19 xbox 360 ISO are given with the ISO file itself step by step.
More over you have two ways to decide from -
We also added some extra features such as -
FIFA 19 xbox 360 free download patches
EA Sports' soccer franchise returns for the 2018 season. On microsofts Xbox 360 platform, FIFA 19 is one of the most fun Soccer
games that was developed by EA Vancouver near 9/28/2018. As it is a part of the FIFA series.


text:
Return to: Department Programs List
This minor is restricted to students working on a teaching major in biology, Earth science, or physics. Students completing this minor are required to demonstrate knowledge, skill, and disposition proficiency through a program portfolio prior to student teaching. Students must pass the WEST-E exam for chemistry to receive a chemistry endorsement.


text:
Top Companies in Bentonville
Wal-Mart Stores Inc.
Address: 702 SW 8th St, Bentonville, AR 72712
Wal-Mart Stores Inc. represents a huge chain of department and warehouse stores. The company has gained plenty of reputation overtime due to the huge discounts and wide variety of special offers. Practically, this...
Browse Cities in Arkansas
Best Places to Work for in Bentonville, AR
The unemployment rates around Arkansas are way above the nationwide ratio – around 7.1%. Despite that, there are still a lot of opportunities for people who actually have a degree. The medical and information technology industries are the most desirable for new graduates.
However, there are plenty of opportunities for people who have not obtained any postsecondary degree. For example, Wal-Mart is a top employer in the area with thousands of employees. Subway, TEK Systems and Mu Sigma are not to be ignored either. In the long run, it is worth noting that any kind of certification will go a very long way in helping a job seeker.


text:
Poetry is the title of a film about a grandmother, from a small town in Korea, reacting to her current life and a recent local tragedy that has involved her family. Despite her age and the odds against her, she continues to try to better her circumstances by taking a poetry class.
The film itself is very visual and always gives just enough character and plot to keep your imaginations grasping for the rest of her; very much like the fragmented narrative style of modern poetry.
The main character is unable to plainly tell us or anybody how she really feels or what she is doing and why. And never does. At the end of the film … well, I'm a natural-born spoiler, so I won't go any further about the contents of the movie. Despite feeling quite melancholy after this 2 1/2 hour long film, I think it is worth seeing. The story and main character are beautifully portrayed and I continued to think about the film for several days after seeing it.
As part of the poetry campaign for this blog, this week's poetry video takes a sample from a poem within this film to entice you to either read the full poem or watch this film.
This is, by far, my favourite poetry video that I have done for the lyw channel on Youtube. This poem so captures the delicate yet enduring quality of the main character of this film. It was a pleasure to choose the quiet black and white photography to breathe with this meditative text. I only wish I could have had the freedom to render the whole poem.
I don't know if it's a copyright thing but the entire poem is difficult to find on the Internet. The one I did find was not faithful to the English translation provided directly in the film. However, when I went back to the film to read the poem faithfully, I found the placement of periods and commas a little confusing there, as well. Most likely, something was lost in translation. We just need to learn Korean, I guess, for the real version of the poem.
Please visit the following link to this week's poetry video, sampling one of Poetry's poems:
blog written by lyw
Image Credits:
- Wildflower image c/o http://f.hatena.ne.jp/uralic/20120816053135
- Dark water with tree image:


text:
The Acacias complex has 25 apartments located on the Jaume I promenade with a capacity of 2 to 5 persons. The maximum capacity is 3 adults with 2 children (never 4 adults). They are fully equipped and offer a wide range of services and comforts designed to make guests feel right at home.
Ideal apartments for holidays with children.
Each apartment has a lounge-dining room and a kitchenette equipped with kitchen utensils, refrigerator, microwave oven and washing machine*, sofa-beds for 3 persons, a separate bedroom with a double bed, a roomy bathroom and a spacious 10m terrace from which to enjoy views of the swimming pool or side sea views.
There are 2 different categories depending on the views and distribution:
- Apartaments Superior: with views of the complex swimming pool or side sea views.
- Apartaments Superior Premium*: with side sea views and a washing machine.
- Hairdryer
- Air conditioning and heating
- Microwave
- Television (International channels are not available)
- Vitroceramic hobs
- Refrigerator
- Kitchen utensils
- *Washing machine (in Superior Premium apartments)
- Obligatory cleaning on day of departure: 10€
- Apartment size: 50m
- Bath towels
- Sheets
- Deposit on check in: 150€.


text:
The marketing team at Members Plus has a difficult job: after all, with competition from big banks with even bigger budgets able to offer endless services, the business of personal banking is more competitive than ever.
It wouldn't be surprising to learn that most consumers shop for a checking or savings account or for a loan through the internet. Members Plus recognized this and knew that in order to stay in the game, they were going to need a compelling web presence to draw in new customers.
The new design offered all of these things along with a clean, professional and, of course, mobile-friendly design. We integrated a mobile banking login front and center for existing customers, as well as a manageable quicklinks section. Initial input that many prospective customers didn't know that credit unions were available to them led us to create an interactive map and callout section to really drive the point home.
On the back-end, we created a whole new way of managing rates using advanced WordPress development that made it easier than ever to update and display rates across the site. The mega menu navigation features promotional areas for an image and tagline that can be changed at any time to display special promotions or new offerings. We created an intuitive branch manager system that can be used to add branch information and add branches as they grow, along with a slick front-end interface to compliment it. Finally, we set them up with secure SSL (an important feature their site did not have before) to put customers mind at ease.
After launch, Members Plus realized the need for ongoing services and signed up to have us build up their social pages and help them come up with a cohesive content strategy for their blog. We explained that these are key ways to drive more traffic to the site and they agreed that every strategy was a worthwhile one. Since then, we've continued to work with them on an ongoing basis and have watched the analytics for the site respond positively to the improved platform and content strategy.
August 2015
- View Project
Like what you see?
Every project starts with us getting to know each other and learning about your company or organization and desired end product.


text:
tag:blogger.com,1999:blog-3694097211268403186.post5619113562861632062..comments2020-08-10T08:39:43.851+01:00Comments on Meet me in Mayfair: Is the beauty industry corrupting childhood?Abigail Franceshttp://firstname.lastname@example.com:blogger.com,1999:blog-3694097211268403186.post-53251950799461223912020-07-23T14:20:39.583+01:002020-07-23T14:20:39.583+01:00I use only high quality materials - you can see th...I use only high quality materials - you can see them at: Beauty
ssshttps://firstname.lastname@example.com:blogger.com,1999:blog-3694097211268403186.post-64267711832888689562015-04-02T04:44:13.043+01:002015-04-02T04:44:13.043+01:00This is a tough one for me because I strongly feel...This is a tough one for me because I strongly feel that children should not wear makeup until they are well into their teens, even though I remember how desperately I wanted to wear more than clear lip gloss at the age of 13. Now that I am older, I have 3 main reasons why I think kids need to wait until they are about 15ish (give or take) to start experimenting with makeup and hair color:
1. You have to be able to purchase and maintain your style. Having personal style is important and being able to express yourself is great, but while your parents should clothe you and hopefully, take your taste into account, it is not up to them to buy every new item that comes out and they certainly shouldn't have to nag you about maintaining your style (like re-dying your hair, cleaning the bathroom after you dropped makeup, etc.) Some kids can handle these things at a younger age, but most need to be in their teens to do it consistently.
2. You have to be mature enough to understand the implications of your style and deal with them maturely. I would never tell anyone what s/he should wear or how they should do their makeup (unless they asked) and I would never judge anyone, but that's just me. Unfortunately, we live in a society wear people will judge and assume certain things about you based on your appearance. Typically, a 16 year old can deal with that appropriately, but a 12 year old might have a hard time.
3. Lastly, it's nice to have things to look forward to in life. If you have your first manicure, kiss, pedicure, facial, massage, pair of heels, date, boyfriend, tube of red Chanel lipstick and Gucci bag all before you turn 13, what will you look forward to at 14? It's not a huge deal, but I didn't get my first facial until I was like 27 and I was way excited. It was something that I'd wanted and looked forward to for a long time and anticipation can be such a wonderful feeling.
I know that there are some very mature tweens and younger teenagers out there, along with some that have a very definite sense of style and I applaud them for having that, but most younger teens still need a few years to get a handle on making good life choices. I remember it being hard, but it's a life lesson that will be worthwhile in the end.
Anonymoushttps://email@example.com:blogger.com,1999:blog-3694097211268403186.post-49683487391419210462015-03-13T22:27:35.865+00:002015-03-13T22:27:35.865+00:00Interesting read and hard to pin point subject in ...Interesting read and hard to pin point subject in terms what should and should not be. I personally feel children should not be indulged to what to do this beyond their age. For example my mom allowed me to wear polish at 12 hesitantly. But when she realized I was unable to maintain the color due to chipping I was not allowed to wear it anymore until I knew how to maintain them.

The point is with every grown up action there is grown up responsibilities. Children should just be that, young and care free! Thanks for sharing.Anonymoushttps://email@example.com:blogger.com,1999:blog-3694097211268403186.post-52147085359905778632015-03-13T18:13:07.285+00:002015-03-13T18:13:07.285+00:00I think wearing make-up is definitely a personal p...I think wearing make-up is definitely a personal preference, I was 12 when I first wanted to wear make-up, however I wasn't allowed until I was 15. While I don't think it made a huge difference in my childhood I am glad I was forced to wait. At a slightly older age I was more confident in myself and better understood the difference between highlighting versus covering my features.
I love how aware your opinion is. Thank you for posting.
-Chelsey // AndEverythingNice Anonymoushttps://firstname.lastname@example.com:blogger.com,1999:blog-3694097211268403186.post-68029863049620043712015-03-11T23:49:51.045+00:002015-03-11T23:49:51.045+00:00Your blog amazing! I do really like it! I know how...Your blog amazing! I do really like it! I know how much time it requires, but you did a really good job! Keep doing it!) I'll be happy to see you in my blog!

Diana Cloudlet
http://www.dianacloudlet.com/
Anonymoushttps://email@example.com:blogger.com,1999:blog-3694097211268403186.post-5202230537340884982015-03-06T10:47:45.313+00:002015-03-06T10:47:45.313+00:00I definitely agree with this. I didn't have my...I definitely agree with this. I didn't have my first phone until I was at least 11 years old and even then I never used it because I much preferred to be outside playing on my bike with my friends. I was about 14 when my mom first took me to Superdrug to buy make-up and even then it was bare minimum and in neutral shades. I think kids nowadays are becoming adults a lot quicker.

Natalie Ann xo // Petal Poppet Blogs ♥Petal Poppethttps://firstname.lastname@example.com:blogger.com,1999:blog-3694097211268403186.post-51473178719847576622015-03-06T01:02:16.053+00:002015-03-06T01:02:16.053+00:00WOOO I GOT MENTIONED HI EVERYONE WOOO I GOT MENTIONED HI EVERYONE Anonymoushttps://firstname.lastname@example.com:blogger.com,1999:blog-3694097211268403186.post-18230670773777381392015-03-05T22:35:35.294+00:002015-03-05T22:35:35.294+00:00I think that this is a really touchy subject and t...I think that this is a really touchy subject and that everyone has there own opinions on it. Personally, I don't really see a problem with boys and girls wearing make-up at a young age as they can do what they like with their allowances etc. but I can see why people are a bit "meh" about it at the same time... The world's a lot different even from when I was a kid (and I'm only 19!!) and I guess this is just how society's evolving to now. So long as children aren't feeling pressured I see no problem with it - it's when they feel inadequate that I start to worry.

Great post!

Chantel Dione | fashion, beauty, music & lifefirstname.lastname@example.com


text:
If you've made it to cloth diapering a Toddler, give yourself a pat on the back! That means you've successfully cloth diapered for over a year - so kudos to you! It's all smooth sailing from here on out, well, kind of.
After cloth diapering for over a year, you probably feel like a pro [and you are]! Or maybe you're just now picking up cloth diapers after your baby has been in disposables for his or her first year of life, and that's fantastic too - you're making a wise choice for your baby, the environment, and your wallet.
Although you won't be changing your toddlers diaper as frequently, you might start to notice that what was working overnight for your baby just might not be keeping your toddler's sheets dry by the morning. I'm sharing with you a couple of overnight cloth diaper solutions for toddlers.
Double Stuff your Cloth Diapers
If you're using a pocket cloth diaper system like Smarti Pants, you may get back with simply double stuffing. You can experiment with this by first stuffing 2 microfiber inserts into your pocket diaper or what I found worked best for my girls is by laying a microfiber insert on top of a hemp insert and stuffing it that way. The microfiber traps liquids fast and the hemp is trim and able to hold more liquids - which gives you a slimmer double stuffed diaper that works twice as hard.
Prefolds with Diaper Covers
Some parents find that certain types of prefolds hold in more liquid, especially at night. You can pair a prefold or folded flat with a diaper cover, like Smarti Pants Diaper Covers to give you a diaper that fits your toddler while absorbing more than a regularly stuffed pocket diaper.
Fitted Diapers with Fleece or Wool Covers
Once my girls hit a certain age, I could no longer stuff their pocket diapers enough - they were too bulky and just couldn't handle the amount of wetting overnight. I chose a Fitted Diaper and Wool Combination simply because a fitted diaper is one in which the entire diaper absorbs, not just the inserts. The Fleece and Wool covers act as a liquid-proof barrier - they hold the wetness into the diaper without letting it escape on your crib sheets. This system can be a bit more costly, which is why I would try the other overnight cloth diaper solutions for toddlers first.


text:
Ginger Grant moves data from Azure Blob Storage into Azure SQL Database using Data Factory:
There are instances where data resides in Azure Blob Storage and the data is needed in a SQL database. For example, if one ran a Machine Learning experiment in Data Factory, the results would be stored in Azure Blob storage, and for analysis purposes, it may make a lot more sense to move the data to SQL database. Moving data around in Data Factory, means writing JSON. In this example we will be using an Azure SQL DB, but it is not essential that the data be stored in Azure. An on-premises SQL Server could also be used, as long as a gateway was added for the connection, the other steps would be the same. There are five different Data Factory elements required to move data from an Azure blob to a database: a pipeline for the data, a data set containing the definition for the blob, a linked service for the blob, a data set containing a definition for the SQL Data, and a linked service to connect to the SQL database.
There's a lot of JSON ahead.


text:
Female High School Student Files Civil Rights Complaint Over Transgender Male Student In The Women's Locker Room! "I Felt Very Violated"
A female Pennsylvania high school student said that her privacy was violated and she was subjected to sexual harassment after a male student who identifies as female looked at her while she was changing in the girl's locker room. Neither student is identified by name in a civil rights complaint filed on March 13 with the U.S. Department of Education's Civil Rights Division. Posted By Persist
Please click the "Report" button below if the video on this page is not working properly.
Comments will only be displayed by verified emails. No Spamming or Racism.


text:
There's a kind of magic that accompanies certain kinds of animation, a sense that, even though it's not strictly realist, it's conveying some greater truth about life that simply wouldn't work with a live action film. My Life as a Zucchini, the Oscar-nominated stop motion film, is enlivened by that magic. Despite a sheen of whimsy, the film is a beautiful and sometimes heartbreaking tale of children coping with profound loss.
The titular Zucchini (Gaspard Schlatter) is actually the nickname of 9-year-old Icare, given to him by his mother. He's introduced alone in his attic bedroom, where he has decorated the walls with his crayon drawings. It's a stark, spare room, and Zucchini's only possessions are a diamond-shaped kite and a supply of continuously replenished beer cans. The simple kite has his drawing of a superhero on one side (his absent father) and a chicken on the other side (his mother said his dad always liked "chicks"). The beer cans belong to Zucchini's mother, an alcoholic who spends her days in front of the TV.
When Zucchini makes a mess of the cans, his mother climbs up the ladder to berate him, and the scared boy closes the ladder door on her head, knocking her down and killing her. When we see Zucchini again, he's at a police station with Raymond (Michel Vuillermoz), the officer assigned to his case. The boy is quiet and reserved rather than weepy—perhaps he knew something like this would happen to his mother one day.
Raymond takes Zucchini to an orphanage, where he's initially bullied by an older child, Simon (Paulin Jaccoud), though their relationship stabilizes when both children realize they've suffered similar losses. Director Claude Barras and the film's writers are sensitively attuned to the current political climate in France and populate the orphanage with children affected by it. One girl's mother has been deported back to an African nation, and a Muslim boy is there because his father was imprisoned for holding up a store for a pair of Nikes. Raymond visits Zucchini periodically, and the other children bombard him with water balloons for being a cop—they've gone through their own horrors of abuse and parental separation, often mediated by the police.
A new child arrives shortly after Zucchini, a girl named Camille who's being deposited by her verbally abusive aunt. Zucchini is instantly taken with her, and the two share an innocent puppy love. The characters in My Life as a Zucchini are designed rather simply, not much more than round heads with little features added on, but the animators are able to create a wealth of emotions from those rudimentary faces. The night after Zucchini first meets Camille, he lays in bed staring at the ceiling with a faint smile that perfectly conveys his new-found infatuation. It's a small but moving detail. Rather than using the animation style to explore the more fantastical possibilities of the medium, My Life as a Zucchini is concerned with conveying the tiniest details of these children's lives. In a Q & A session following a screening at Los Angeles's Nuart Theater, Barras explained that the purpose of the animation was to add a sense of poetry to the movie, as well as softening its rougher edges. It might have been possible to craft a live action version of this film, perhaps in the style of Ken Loach's Kes, but that film would have been an oppressive mess without the addition of Zucchini's poetry.
The movie's veracity is aided by its cast of non-professional child actors. They lack the annoying self-awareness that often infects professional child actors or the adult voiceover artists who impersonate them. These actors downplay their emotions in a more realistic fashion. Instead of doing traditional voiceover recordings like most animated films, six weeks were spent filming a live-action version of the movie prior to any animation. The animators were able to use the actors' own expressions to craft their stop motion doppelgangers.
Although spare in decoration, the culture of the orphanage rings true. Anyone who has visited a similar institution will recognize the display on the wall where children mark their mood for the day, both a warning and a guide for their caretakers. Barras spent three weeks visiting an orphanage to prepare for the film, and one of the writers spent a whole year observing to ensure that it was neither the Dickensian hellhole so often shown in American films, nor some kind of Peter Pan-like utopia for orphaned children.
At times the film abandons it's more realist tendencies, especially in sequences involving clandestine recordings of an abusive caretaker, but most of the movie is filled with more bittersweet aspects of life. The film lacks the digital glitter of its better known Oscar competitors, but the subtle pleasures of My Life is a Zucchini have their own special charms.


text:
Are you looking for a natural solution that actually works?
Are you someone who doesn't like taking medications? Maybe you've tried other natural remedies but they haven't worked. Or maybe the medications you are taking aren't working well enough. It's very common, especially with people suffering from conditions like chronic pain, autoimmune disorders, arthritis, migraines, neuropathy, high blood pressure, and others listed below. Often people are not given a good solution from conventional doctors OR other natural methods.
That's where we come in. Chinese Medicine is based on a 3,000 year old science of determining the actual cause of your problem and how to address it with specific and effective methods. Many people have a laundry list of disease names and diagnoses but ultimately still don't feel good despite all the testing, medications, and conventional treatments.
Chinese Medicine actually gives you something to do about it. We help people with natural treatments like herbal medicine, acupuncture, cupping, and shiatsu. Our patients report that our treatment methods work better than anything they've tried for years. They report less pain, more energy, and are able to get back to doing the activities they love.
We help you find an herb instead of a pill.
Natural medicine that works.
Acupuncture for Veterans
We help Veterans who are dealing with chronic pain, injuries, PTSD, anxiety, insomnia, and more. Many Veterans have found relief at our clinic using acupuncture to treat problems ranging from low back pain to fibromyalgia to opioid painkiller addiction.
We accept VA insurance through Triwest and the Boise VA. Most Veterans are eligible to have 20 acupuncture visits covered per year. Ask your local VA doctor for a referral to our clinic for acupuncture.
When you need Chinese Herbs, you need them to work. That's why we specialize in Chinese Herbal Medicine. We source only the highest quality organic and lab-tested herbs because we care as much about safety and efficacy as you do.
Our practice places a heavy emphasis on Chinese Herbal Medicine, as herbal treatment is often necessary to treat chronic, severe, and recalcitrant problems. Traditional administration of high-quality herbs in traditional dosages frequently yields successful results where other methods have failed.
Chronic Pain
Lyme Disease
Meet Sean Dugan LAc, MAOM
Chinese Medicine Specialist
When you need Chinese Herbs, you need them to work. That's why Sean has dedicated his career to the mastery of Chinese Herbal Medicine.
Sean is a board-certified Chinese Medicine Practitioner and Licensed Acupuncturist with a Master's Degree in Acupuncture and Oriental Medicine from the Oregon College of Oriental Medicine. He is licensed by the State of Idaho and certified by the National Certification for Acupuncture and Oriental Medicine.
Sean is a classically trained herbalist who specializes in internal medicine for the treatment of chronic disease. He practices Jing Fang Classical Chinese Medicine and Jing Gui medical pulse diagnosis. Sean sees patients for a wide variety of conditions, including cardiovascular disease, respiratory illness, autoimmune conditions, fibromyalgia, Lyme disease, and neurological conditions.
Sean is trained in Xing Nao Kai Qiao Acupuncture technique, the most researched and effective acupuncture method for neurological
conditions including stroke rehabilitation, concussion and TBI, tremor, Parkinson's, and MS. Sean also utilizes Orthopedic Acupuncture techniques for sport's medicine and Japanese Acupuncture techniques for those needing a more gentle approach. Sean also utilizes non-needling therapeutic techniques such as cupping, guasha, moxibustion, and Tuina massage.
Healthy digestion is essential to feeling good. Acid reflux, GERD, chronic loose stool, constipation, IBS, intestinal parasites, Clostridium difficile infection are among a few of the digestive problems we've been able to help with Chinese Medicine. Herbs, acupuncture, and sometimes dietary changes and we'll have you feeling much better. "I'm having regular, spontaneous, bowel movements for the first time in 20 years!" is a frequent side effect of Chinese Medicine treatment.
We all know we need it, yet many of us don't get enough sleep or don't get quality sleep. Whether they come in seeking help with sleep or not, most patients find themselves sleeping better with Chinese Medicine treatment. Insomnia, sleep apnea, nightmares, bruxism, bed-wetting, are all sleep problems that can be helped with Chinese Medicine.
Men's Health
Most men don't like to talk about their problems. We get it, and we don't mind. All you have to do is show up, take your herbs, and you'll be feeling better soon. We're here to fix problems, and talking about them is not required unless you want to. We know that you are strong enough to bear the pain, but we are here so you don't have to.
Cardiovascular Health
Did you know that all the blood in your body pumps through your heart every 60 seconds? We know how vital heart health is to our lives. Yet heart disease is still the leading cause of death worldwide. That's why we place such a strong emphasis on cardiovascular health in all our patients. Believe it or not, Chinese Medicine can detect pathological changes in the cardiovascular system decades before modern testing methods such as the EKG. Many people have pre-clinical heart problems that will go untreated until they get bad enough to require surgery or drugs. With Chinese Medicine you don't have to wait, we can help now. We have helped patients with hypertension, arrhythmia, angina, atrial fibrillation, mitral valve prolapse, palpitations, edema, varicose veins, and shortness of breath, to name a few.
Chronic Pain
Neck pain, back pain, headaches, arthritis, fibromyalgia, hip pain. If you have any type of pain, acupuncture and herbal medicine are some of the most effective treatments for pain available. We get excellent results with acute and chronic pain of all types. Speed up post workout recovery time, and help training related injuries to heal faster.
Mental Health
We all recognize stress as a major factor in our lives today. Many of us struggle with feeling depressed, overwhelmed, anxious, irritable, angry. The good news here is you'll never hear a Chinese Medicine practitioner say "it's all in your head." Because it's not. Chinese Medicine has always recognized that there is no separation between our mind, body, and emotions. If you feel like something is wrong, then something IS wrong. Chinese Medicine treats the mind and emotions through treating the body, often with dramatic results.
Para hacer una cita en español, llame al 208 629 8932


text:
Tuesday, April 8, 2008
John Cena Still The Future?
John Cena is not the champion right now. However, I couldn't help but notice that on Raw he got a piece of the championship pie handed to him.
The crowd at each arena is not fully behind John Cena anymore, and it's odd to see that he's in the fold chasing the title.
I think it's ridiculous that once again, at Backlash, we're going to get a multi-man main event with little to no implications for the future, outside of the crowning of a potential John Cena title reign.
Or am I completely wrong?
Think about it for a moment.
Why would Orton, HHH, or JBL be the champions?
Orton has had the title for a long time now, and I for one am tired of seeing a horrible heel champion. Sure he has some microphone skills, but his matches are plagued with lackluster endings, especially ones that involve a stupid kick to the head in the most impromptu times.
JBL has been sitting on the sidelines for so long, that it doesn't make sense for him to clothesline himself into a world title reign. He's been to the mountain top before, this time is not going to be a great moment in WWE history if it is the intentions of the WWE to hand over the title to JBL.
HHH? HHH is due a title reign, but he hasn't been in any memorable feuds to qualify him for the job. HHH is solid, but he just lacks the emotion and face to work on his heel position...I understand he's face right now, but he's a poor face if the heel is nothing more than a young champion with a kick to the head.
I might be wrong.
However, the amount of merchandise that John Cena can move as a champion is not really rivaled by any other potential champion.
He's also young enough to move into another memorable feud.
The ultimate example of what they could do with John Cena is to turn him heel, out of frustration. Have him lose at Backlash, lose the next night on Raw, then cheat his way back into the fold! Cheat his way in, hit someone with a chain, get caught and start rapping again and not caring with the kiddies think. Stop saluting people, stop trying to be the wholesome one, and just do what he used to do...when he got over with the adults, despite his lackluster move set.
Liked this post? Want to help me keep blogging? Buy a dvd or two from Amazon.com and save up to 75% off WWE merchandise, and get free shipping while you're at it; just click HERE
Posted by Unknown at 9:11 PM
Labels: hhh, jbl, john cena, monday night raw, randy orton, triple h, wwe, wwe championship
Subscribe to: Post Comments (Atom)


text:
Liberty and Lipscomb will square off for the first time since meeting in the 2019 ASUN Championship as the Flames close out its three-game homestand. The Flames remain the only team in the ASUN unbeaten during conference play as Lipscomb starts the week 2-2 in the ASUN.
Gameday Central Links
Game Notes
Live Stats
Live Video
Live Audio
Purchase Tickets
Liberty vs. Lipscomb
Game Date: Saturday, Jan. 18th
Game Time: 7 p.m. (ET)
Live Video: ESPN+
Play-by-Play Broadcaster: Matt Warner
Color Analyst: Paul Nazigian
Sideline Reporter: Emily Austen
Live Audio: LFSN Radio (107.5 FM)
Play-by-Play Broadcaster: Alan York
Liberty Notables
Like Us on Facebook
Follow Us on Twitter
Follow Us on Instagram


text:
$26.23 $34.97
What's special about this yarn?
This bring the sparkly elegance of the night sky to life in your hands, with its subtle elegance. The Angelina compliments the black merino and isn't garish like some blends with sparkles can be.
How does it feel?
Its soft and grounding, like lying on the ground on a cool summer night gazing up at the stars.
18.5um merino from South Africa
Non-Mulsed merino
18.2um Rambouillet from America
Angelina nylon from Europe


text:
The Hotel Royal Orchid, tucked neatly and conveniently away from the hustle and bustle of Nairobi city, is a definite hidden gem worth exploring. Being part of the Royal Orchid Group, the five star hotel is home to two restaurants, a bar and a 24 hour cafe. They invite you to enjoy their delicately prepared meals served at their restaurants and unwind your evening as you enjoy the fantastic view of the city from the roof top.
Located on the ground floor of Azure Towers, this restaurant offers a buffet set up providing multi-cuisine delicacies that are guaranteed to satisfy your taste buds. It boasts of both an inside and outside setting, with the outside setting overlooking the swimming pool.
At Tiger Trail, you get to sample only the finest Indian Cuisine from a delicate balance of succulent food coupled with a complete fine dining experience and authentic ambiance.
This is home to the most delicious pastries set to make your sweet tooth happy. Sip on their quenching beverages which range from perfectly blended tea, coffee and herbal infusions as you pair them up with assorted sandwiches. Also, keep up to date with all your work, or social media with their free Wi-Fi.
Catch up with the boys or girls at this elegantly designed watering hole situated on the first floor of the hotel. Have fun and memorable moments as you sip on their special brews from their bar list.
More interesting reads:
Brand spanking new bars and restaurants in Westlands


text:
Abu Dhabi Municipality says Al Falah roadworks project to be completed
Project is part of an ongoing development drive in Abu Dhabi and its suburbs
Abu Dhabi City Municipality (ADM), in cooperation with Musanada, the Abu Dhabi Public Services Company, will fully complete all infrastructure and internal roads projects that started in Al Falah last year, by the end of June, it has announced.
According to a report by WAM, the project is part of the ongoing development drive in Abu Dhabi and its suburbs, as part of the plan to satisfy community needs in the area. It is also one of the projects that aims to realise the overall vision of the emirate, bring happiness to the community, and bolster the reputation of Abu Dhabi, the report added.
The project includes the construction of three-lane, two-way roads between the old and new Al Falah cities, in addition to four intersections in the southern and western part of the new part of the city. It also includes the completion of road networks surrounding the Al Falah residential area, in addition to other associated works, such as rainwater drainage, lightening, intersections and light signals.
The infrastructure works in Al Falah will cost more than $11.2 million and will provide roads and infrastructure services to existing and new residential land plots, thus enhancing the quality of life and services on offer to the populace, the report said.
The Al Falah area is part of many areas targeted with projects aimed at upgrading infrastructure in the foreseeable future, tailored to cater to the economic boom and rising demographic growth rates witnessed by the emirate of Abu Dhabi at all levels.


text:
Hi, my name is Jory Teltser and I am an aspiring professional photographer. I consider myself an expert bird watcher and a highly skilled wildlife photographer. Recently, I have started to expand my interests into the world of landscapes, portraits, and street photography.
Behind birding, photography is my biggest passion and love. I have opened up my creative side to enjoying nature, and finding compositions in it's overflowing beauty. I am now taking my skills as an photographer to the next level, by trying to share my work with the world and giving people an opportunity to be expressed through images.
Copyright © 2018 Jory Teltser Photography - All Rights Reserved.


text:
Seven Seas, Odessa, Ukraine
- Free continental breakfast and free WiFi
- Collect stamps
Staff is friendly, properties clean and beautiful, Breakfast is wonderful, staff will help you with any of your questions and and assist you making travel plans or tours .
Seven Seas, Odessa, Ukrainefrom NZ$134
- Deluxe Double Room (Celestial)
- Deluxe Double or Twin Room (New York)
- Deluxe Double Room, Terrace (Marrakech)
- Junior Suite, Terrace (Ethno)
- Deluxe Suite, 1 Bedroom (Montenegro)
- Deluxe Double or Twin Room (Provence)
- Deluxe Double or Twin Room (Marseille)
- Deluxe Suite, 1 Bedroom (Shangri-La)
More great choices
What's around Seven Seas, Odessa, Ukraine
- In Prymors'kyi district
- Arcadia Beach - 23 min walk
- Gold Coast Beach - 32 min walk
- Uspensʹkyy Cholovichyy Monastery - 2.8 mi / 4.5 km
- City Garden - 6 mi / 9.7 km
- Potemkin Stairs - 6.2 mi / 10.1 km
- Memorial of the heroic defense of Odessa. 411-I battery - 4.5 mi / 7.2 km
- Odessa Academic Theater of the Musical Comedy - 4.7 mi / 7.6 km
Getting around
- Odessa (ODS-Odessa Intl.) - 23 min drive
- Free airport shuttle
At a glance
Key facts
Hotel size
- This hotel has 8 rooms
- This hotel is arranged over 3 floors
- Check-in time 3:00 PM-11:00 PM
- Check-out time is noon
24-hour airport shuttle service is available on request. Fees may apply. Contact the property in advance to make arrangements.
Required at check-in
- Credit card, debit card, or cash deposit required for incidental charges
- Government-issued photo ID may be required
- Minimum check-in age is 18
- If you require a visa to enter the country, your property may be able to help with the supporting documents needed to obtain one *
Travelling with others
- One child stays free when occupying the parent or guardian's room, using existing bedding
- Pets not allowed
- Free WiFi in public areas
- Free WiFi in rooms
- Free airport shuttle (available 24 hours) *
Payment types at the property
- Cash
In the hotel
- Free continental breakfast daily
- Room service (during limited hours)
- Barbecue grills
- Outdoor pool
- One meeting room 1
- 24-hour front desk
- Tours/ticket assistance
- Laundry facilities
- Free newspapers in lobby
- Luggage storage
- Number of buildings/towers - 1
- Year Built - 2017
- Safe-deposit box at front desk
- Designated smoking areas (fines apply)
- Picnic area
- Terrace
- Library
- English
- Russian
In the room
- In-room climate control (air conditioning)
- Minibar
- Coffee/tea maker
- Bathrobes
- Slippers
- Iron/ironing board (on request)
- Down comforter
- Soundproofed rooms
- Premium bedding
- Sleep Number by Select Comfort mattress
- Yard
- Individually decorated
- Individually furnished
- Private bathroom
- Separate bathtub and shower
- Bidet
- Free toiletries
- Hair dryer
- 42-inch flat-screen TV
- Satellite TV channels
- Desk
- Free WiFi
- Free local calls
- Refrigerator
- Daily housekeeping
- In-room safe
Recommended hotels
Seven Seas, Odessa, Ukraine's small print
Also known as
- Seven Seas Hotel Odessa
- Seven Seas Odessa
- Seven Seas Hotel
- Seven Seas Odessa
- Seven Seas Hotel Odessa
If you require a visa to enter the country, your property may be able to help with the supporting documents needed to obtain one. To learn more, you can reach out to the property via the contact details included on your booking confirmation. The property may charge for this assistance, even if you end up cancelling your reservation. All arrangements are solely between the property and yourself.
This property does not have elevators.Guests must contact this property in advance to reserve cribs/infant beds.
For transfers, guests must contact the property 24 hours prior to arrival at the number on the booking confirmation.This property only accepts cash for all bookings and onsite payments.
Mandatory fees
You'll be asked to pay the following charges at check-in or check-out:
- A tax is imposed by the city: UAH 20.87 per person, per night. This tax does not apply to children under 18 years of age.
Cash deposit: UAH 3000.0 per stay
Optional extras
Rollaway beds are available for UAH 770.0 per night
We have included all charges provided to us by this property. However, charges can vary, for example, based on length of stay or the unit you book.
FAQs for Seven Seas, Odessa, Ukraine
Does Seven Seas offer free cancellation for a full refund? Yes, Seven Seas does have fully refundable rooms available to book on our site, which can be cancelled up to a few days before check-in. Just make sure to check this property's cancellation policy for the exact terms and conditions. Is Seven Seas currently open? This property is closed from June 16 2020 to August 31 2020 (dates subject to change). Is there a pool at Seven Seas? Yes, there's an outdoor pool. Are pets allowed at Seven Seas? Sorry, pets aren't allowed. What are the check-in and check-out times at Seven Seas? You can check in from 3:00 PM to 11:00 PM. Check-out time is noon. Is airport shuttle service offered by Seven Seas? Yes, a free airport shuttle is available.
Is the description of this property not correct? Tell us