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See you next week
Week 1 complete
Congratulations on getting this far.
By now you will have an understanding of the key concepts of big data analytics, where big data is coming from, and why it is so challenging to analyse. You will also have an appreciation of some of the implications of big data analytics.
Coming up next week
Join us next week to discover how big data analytics can be applied to solve problems in different industries. We'll look closely at case studies in the areas of:
- environment
- sport
- health, and
- business.
These examples should start you thinking about the possibilities big data analytics presents for all areas in our future.
Your task
What you are looking forward to most next week?
Share your answers in the comments.
© Griffith University


text: Shortly after the time we found out that we were to expect a baby at the end of the year, my grandfather died of a heart attack. My grandfather, or 'Papa' as us grandkids called him, had dreams of an art career in his youth, but the Great Depression and the demands of a growing family made it necessary to find more practical employment, yet he continued to throw his creative impulses into his everyday life, through creative carpentry, and an oil painting hobby (I still have his homemade art painting box where I store my own oil paints). I wish he could've lived to see me on my feet as a freelance artist, and I like to think he would've gotten a certain amount of satisfaction out of it. I did this pastel portrait of him shortly after his death and it still hangs in my grandmother's bedroom (she eventually passed away at a ripe old age of 101 many years later). I have many fond memories of summers spent at their Whitefish Bay cabin on Lake Superior near Paradise Michigan (me and my brother were usually shipped up there for the last half of the summer to escape hay fever allergies), and of wild blueberry picking and hunting for agates on the shore.

subset: commoncrawl


text: Expertise and technique are the two main keys to any great photograph. In case you're searching for a wildlife photography course, we'd recommend the best place to start is Institute of photography. Now you may ask why? The reason to take courses from this institute is simple, its learning process is different from the other institute in the market. It's


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Real-Time Returns
The year was 1988. Bush No. 1 was warring with Dukakis, Guns N' Roses was just hitting the rock scene and Phil Marsillio was looking to modernize his family-owned, high-end appliance store. That decision led to the adoption of computer-run, point-of-sale software from Storis, which, at the time, was in its infancy. Today, nearly 20 years later, Marsillio considers it one of his most profitable business decisions.
"As far as Vision R8 (the latest version of the Storis platform), it's superior to everything I've seen," said the co-owner of Fairfield, Conn.'s Marsillio's Appliances, which has been in business for over 62 years. "Over the years, it's evolved to know what customers are asking for and it carries it out."
Vision R8 is an integrated, real-time system that connects all the aspects of a retail business—from POS to accounting—in a Windows-based environment with the goal of improving efficiency, lowering operating costs, enhancing customer service and increasing profitability. The software includes inventory control, merchandising, accounts receivable, finance receivables, customer service, an executive dashboard, EIS reporting and custom floor tags.
- Companies:
- Microsoft Corporation
- Storis
- Places:
- Fairfield, Conn.
- Windows


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We go again.
The Gameweeks continue to come thick and fast in Fantasy Premier League with managers given little time to collect their thoughts. Fantasy Football Scout has been a hive of activity in the last 24 hours with writers and contributors working furiously to bring you the latest team news, stats, and opinion.
On Wednesday evening Tammy Abraham overcame a hip injury to deliver a fifth double-figure haul of the season against Aston Villa. The Chelsea forward is once again a small doubt heading into Gameweek 16 although it's unlikely anyone is selling this time around. Neale's team news report included the following quote from Frank Lampard:
Tammy Abraham is good, he hasn't done much in between games. We'll make sure of that in the morning but at the moment he's good.– Frank Lampard
Jurgen Klopp surprised fantasy managers by dropping two of his talismanic front three as both Roberto Firmino and Mohamed Salah sat out the Merseyside derby. Post match quotes from the Liverpool manager did little to assuage fears of further rotation:
I have to show the respect for the boys and I've said a lot of times how much I like the squad and now we have to use it. We think the game will be really intense and for this you need kind of fresh legs and we try to bring them in.– Jurgen Klopp
When quizzed by Andy on the likely Liverpool starting XI Neale nimbly evaded the question. However after his 15-point haul against Everton, Sadio Mané is included in this week's Scout Picks. Sam, in her video review of Gameweek 15, agreed with David Wardale's conclusion that:
Where Salah was once the undroppable driver of the Reds' attacking juggernaut, Mane is now the man behind that particular wheel, and ignoring him is to play a very perilous game indeed.– David Wardale
Dare Klopp rest him?
Another player to miss the midweek round of fixtures was Anthony Martial. Rotation's Alter Ego revealed in his team news summary that the Frenchman is now fit again although unlikely to start in the Manchester derby.
With understandable concerns over rotation and injuries during this busy period, Greyhead investigated which of the Great and the Good had made the best use of their benches. The answer is Neale, who has gained an average of 5.4 points per auto-sub. A hot topic on benches followed.
Cheap defenders may come to our rescue. The talking point on this week's Scoutcast centered around the proliferation of budget options. Joe, Andy and Az debated whether we should even be opting for four at the back.
Although there wasn't such good news for the poster boy of defensive value this season – John Lundstram – when Sheffield United lost at home to Newcastle on Thursday evening. Reflecting on that defeat Neale suggested that conceding an early goal changed the dynamic of the match:
Sheffield United averaged 41.1% possession and 137.9 passes in the opposition half per Premier League match before Thursday night. Against Bruce's backs-to-the-wall Geordies, those figures shot up to 72.7% and 362 respectively. All of a sudden the boot was on the other foot and a Blades side so adept at soaking up pressure and hitting teams with dynamic, direct attacks had to find a way of patiently breaking down a five-man defence.– Neale Rigg
Lundstram owners will be hoping for a more open game when the Blades travel to Norwich City this weekend. And Joe is fairly confident they will be in luck. On the Scoutcast he revealed that no team has conceded more chances at home than Norwich in their last four home matches (17):
Norwich at home are far worse than they are away.– Joe
Looking for opportunities is a key component of David's Captain Sensible article. With Vardy and Mané neck and neck in the captaincy poll – in addition to his normal stats tables – David uses the members comparison tool to assess the pair. Opponents Aston Villa and Bournemouth are also scrutinised.
Manchester City are narrowly top of the latest DS stats, fixtures and clean sheet odds brought to us by G-Whizz. And Raheem Sterling is top of this week's RMT predictions. However Pro Pundit Zophar has already sold the 24-year-old and is busy plotting on how to spend the cash.
In terms of captaincy, Zophar voiced concerns Mane's playing time as his reason for sticking with Vardy:
Captaincy is likely to remain on Jamie Vardy. Sadio Mané playing 90 minutes on Wednesday has me worried he might be benched against Bournemouth keeping in mind their Champions League game on Tuesday.– Zophar
Indeed in his Gameweek 16 forecast Pep Pig thinks that Salah will bounce back after his midweek benching. He also picks Marcus Rashford to shine in the Manchester derby. It was pointed out on the Scoutcast that Rashford has scored against top six teams he's faced this season – Chelsea, Leicester, and Liverpool.
Although United-fan Andy was more cautious, suggesting that it was his side's domination of the Spurs midfield that afforded Rashford the opportunities to score twice. He doesn't expect a similar domination against Manchester City.
To view all our discussions please visit our Hot Topics page, and if you've got some analysis, scout reports or features brewing, be sure to visit our community section to submit your article.
If you're a member and are yet to get to grips with the custom statistics tables, you can view this movie for an explanation of how they work. Also, remember to tag them so that people can find the tables with ease.
Community Competitions
For a full update on all FFS Mini-Leagues and Community Competitions, please click here to read the latest round-up.
For those interested in the Head-to-Head Leagues, the main page can be linked to here. The fixtures for Gameweek 16 can be found below:
Use the drop-down menus to show your league and division. To find out which league and division you are in, follow the instructions on the main Head-to-Head page.
Once the Gameweek starts, if you click on your opponent's name you will be linked to their team on the FPL website.
Best of luck for Gameweek 16!


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active beauty
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We don't let our age define our body's physical capabilities, yet we let it define the confidence of our appearance. From wrinkle reducing creams and serums to supplements, we waste time, energy and money sifting through the vast array of anti-aging products to develop an effective, daily routine.
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"The Active Beauty™ one-stop solution brings superior results with the science to back it up."


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Gibbous ♋ Cancer
Moon phase on 2 February 2023 Thursday is Waxing Gibbous, 12 days young Moon is in Cancer.Share this page: twitter facebook linkedin
Waxing Gibbous is the lunar phase on . Seen from Earth, illuminated fraction of the Moon surface is 91% and growing larger. The 12 days young Moon is in ♋ Cancer.
Friday Fri
Saturday Sat
Sunday Sun
Monday Mon
Tuesday Tue
Wednesday Wed
Thursday Thu
Previous main lunar phase is the First Quarter before 4 days on 28 January 2023 at 15:19.
Moon rises in the afternoon and sets after midnight to early morning. It is visible to the southeast in early evening and it is up for most of the night.
Moon is passing about ∠8° of ♋ Cancer tropical zodiac sector.
Lunar disc appears visually 9.1% narrower than solar disc. Moon and Sun apparent angular diameters are ∠1777" and ∠1947".
Next Full Moon is the Snow Moon of February 2023 after 3 days on 5 February 2023 at 18:29.
There is medium ocean tide on this date. Sun and Moon gravitational forces are not aligned, but meet at very acute angle, so their combined tidal force is moderate.
The Moon is 12 days young. Earth's natural satellite is moving from the first to the middle part of current synodic month. This is lunation 285 of Meeus index or 1238 from Brown series.
Length of current 285 lunation is 29 days, 10 hours and 13 minutes. This is the year's shortest synodic month of 2023. It is 4 minutes shorter than next lunation 286 length.
Length of current synodic month is 2 hours and 31 minutes shorter than the mean length of synodic month, but it is still 3 hours and 38 minutes longer, compared to 21st century shortest.
This New Moon true anomaly is ∠360°. At beginning of next synodic month true anomaly will be ∠15.6°. The length of upcoming synodic months will keep increasing since the true anomaly gets closer to the value of New Moon at point of apogee (∠180°).
11 days after point of perigee on 21 January 2023 at 20:58 in ♑ Capricorn. The lunar orbit is getting wider, while the Moon is moving outward the Earth. It will keep this direction for the next day, until it get to the point of next apogee on 4 February 2023 at 08:55 in ♌ Leo.
Moon is 403 413 km (250 669 mi) away from Earth on this date. Moon moves farther next day until apogee, when Earth-Moon distance will reach 406 476 km (252 572 mi).
4 days after its ascending node on 28 January 2023 at 16:05 in ♉ Taurus, the Moon is following the northern part of its orbit for the next 9 days, until it will cross the ecliptic from North to South in descending node on 12 February 2023 at 07:31 in ♏ Scorpio.
4 days after beginning of current draconic month in ♉ Taurus, the Moon is moving from the beginning to the first part of it.
At 08:18 on this date the Moon is meeting its North standstill point, when it will reach northern declination of ∠27.511°. Next 14 days the lunar orbit will move in opposite southward direction to face South declination of ∠-27.629° in its southern standstill point on 16 February 2023 at 14:36 in ♐ Sagittarius.
After 3 days on 5 February 2023 at 18:29 in ♌ Leo, the Moon will be in Full Moon geocentric opposition with the Sun and this alignment forms next Sun-Earth-Moon syzygy.


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Posts for: October, 2018
Along with thumb sucking, childhood teeth grinding is one of the top concerns anxious parents bring to their dentists. It's so prevalent, though, many providers consider it normal behavior—the sleep-disturbing sound it can generate is often the worst consequence for the habit.
But that doesn't mean you should brush aside all concern, especially if the habit continues into late childhood. Long-term teeth grinding could eventually damage the teeth and gums.
Teeth grinding (or clenching) is the involuntary movement of the jaws when not engaged in normal functions like chewing, speaking or swallowing. The action often produces higher than normal chewing forces, which over time can accelerate tooth wear, cause fractures, or contribute to loose teeth, all of which could increase the risk of dental disease. While it can occur at any time it's most common among children during nighttime sleep.
While stress is the usual trigger for teeth grinding in adults, with young children the causes for the habit are more complex and less understood. Most doctors hold to the theory that most pediatric teeth grinding arises during shifts from lighter to heavier, rapid-eye-movement (REM) sleep. The child's immature neuromuscular chewing control may engage involuntarily during this shift. Teeth grinding is also prevalent among children who snore or mouth-breathe, or who take anti-depressant medication.
But as mentioned before, there's usually no cause for concern unless the habit persists beyond about age 11. If the habit isn't fading, you should speak to your dentist about ways to reduce it or its effects. One way is with a custom-made night guard worn during sleep. The smooth, plastic surface of the appliance prevents teeth from making solid contact with each other during a grinding episode.
You might also seek treatment from an ear, nose and throat (ENT) specialist if your child is having issues with airway obstruction, which could also relieve teeth grinding. And children experiencing stressful situations or events may find relief both emotionally and physically from psychological therapy.
At younger ages, you can safely regard your child's grinding habit as normal. But if it persists, it's worth looking for ways to reduce it.
If you would like more information on your child's teeth grinding habit, please contact us or schedule an appointment for a consultation. You can also learn more about this topic by reading the Dear Doctor magazine article "When Children Grind Their Teeth: Is the Habit of 'Bruxism' Harmful?"
There's something universal about thumb sucking: nearly all babies do it, and nearly all parents worry about it. While most such worries are unfounded, you should be concerned if your child sucks their thumb past age of 4 — late thumb sucking could skew bite development.
Young children suck their thumb because of the way they swallow. Babies move their tongues forward into the space between the two jaws, allowing them to form a seal around a nipple as they breast or bottle feed. Around age 4, this "infantile swallowing pattern" changes to an adult pattern where the tip of the tongue contacts the front roof of the mouth just behind the front teeth. At the same time their future bite is beginning to take shape.
In a normal bite the front teeth slightly overlap the bottom and leave no gap between the jaws when closed. Â But if thumb sucking continues well into school age, the constant pushing of the tongue through the opening in the jaws could alter the front teeth's position as they erupt. As a result they may not fully erupt or erupt too far forward. This could create an open bite, with a gap between the upper and lower teeth when the jaws are closed.
Of course, the best way to avoid this outcome is to encourage your child to stop thumb sucking before they turn four. If, however, they're already developing a poor bite (malocclusion), all is not lost — it can be treated.
It's important, though, not to wait: if you suspect a problem you should see an orthodontist for a full evaluation and accurate diagnosis. There are even some measures that could discourage thumb sucking and lessen the need for braces later. These include a tongue crib, a metal appliance placed behind the upper and lower incisors, or exercises to train the tongue and facial muscles to adopt an adult swallowing pattern. Often, a reward system for not sucking their thumbs helps achieve success as well.
Thumb-sucking shouldn't be a concern if you help your child stop before age 4 and keep an eye on their bite development. Doing those things will help ensure they'll have both healthy and straight teeth.
If you would like more information on thumb sucking, please contact us or schedule an appointment for a consultation. You can also learn more about this topic by reading the Dear Doctor magazine article "How Thumb Sucking Affects the Bite."
A tooth with deep decay is in real peril. If the disease isn't stopped, it can eventually infect the bone and greatly increase the risk of losing the tooth. But tooth decay removal and a root canal treatment can stop advancing decay and resulting infection in its tracks.
During this common procedure we first drill into the tooth to access the inner pulp. After removing the infected pulp tissue, we disinfect and fill the empty chamber and root canals with gutta percha. We then seal the tooth and crown it to protect against re-infection.
But while most root canals are successful and long-lasting, sometimes the tooth becomes re-infected. Here are 3 factors that could affect the long-term success of a root canal treatment.
Early treatment. Like many health problems, the sooner we detect decay and treat it, the better the outcome. A tooth in which the infection has already advanced beyond the pulp is at greater risk for re-infection than one in which the infection is localized in the pulp. Keeping up your regular dental visits as well as seeing the dentist at the first sign of abnormality—spots on the teeth or pain—can increase your chances of early diagnosis.
Tooth complications. Front teeth with their single roots and canals are much easier to access and treat than a back molar with an intricate root canal network. Root canals can also be extremely narrow making them easy to miss during treatment. In cases like this the expertise and advanced equipment of an endodontist (a specialist in root canal treatment) could help increase the odds of success in complex situations.
The aging process. Teeth do wear over time and become more brittle, making them increasingly susceptible to fracture. A previous root canal treatment on an aging tooth might also increase the fracture risk. To avoid this, it's important for the tooth to receive a crown after the procedure to protect the tooth not only from re-infection but undue stress during chewing. In some situations, we may also need to place a post with a bonded composite buildup within the tooth to give it extra support.
Even if a tooth has these or similar complications, a root canal treatment may still be advisable. The benefits for preserving a decayed tooth often far outweigh the risks of re-infection.
If you would like more information on root canal treatments, please contact us or schedule an appointment for a consultation. You can also learn more about this topic by reading the Dear Doctor magazine article "Root Canal Treatment."


text: One way to appreciate the impact of communities in organizations is to look at how the community and its leadership are connected into the overall structures of the sponsoring organization. So, where does community reside in organizations? We took a look in our SOCM 2017 research. There's … [Read more...]


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2017 Volvo XC40 Release DateCategory : Volvo
2017 Volvo XC40 Review, Release and Price – Welcome to Bestautorelease.com, we will provide updated information about the release of car 2017 Volvo XC40. Volvo car is one brand that is famous all over the world, every car that was released has always brought great changes and awesome. Examples are cars 2017 Volvo XC40 released in 2017. We will also review about the price, interior, exterior and engine of the 2017 Volvo XC40 Release Date.
Their cabins are comfortable, design is elegant and reliable engines are under the hood. Also, safety is at top level, which is recognized by buyers, and that is why company is very popular. Same characteristics are for all classes in which company has its vehicles, and so it is with new compact crossover. Built on the new platform, 2017 Volvo XC40 will be attractive and stylish. With it, company will complete their crossover class, adding this small SUV under bigger vehicles, XC60 and XC90.
Though the vehicle is primarily targeted to the Indian market, Volvo will also offer the model in other markets world over. The new Volvo has been significantly redesigned to enable it compete with the segment's top models. Volvo is almost finalizing development of the XC40 and already the model is undergoing testing though disguised in the smaller V40 body.
2017 Volvo XC40 Price
The latest price of 2017 Volvo XC40 has been set at $32,000 – $40,000 based on the current dollar exchange rate. If there are changes in prices in 2017 Volvo XC40 we will do an update on this article, so keep following our blog Bestautorelease.Com.
2017 Volvo XC40 Specs
Below we have summarized some of the reviews about the features of the car exterior and interior 2017 Volvo XC40. The car is expected to be a comfortable car for the ride by the community.
Development of the 2017 Volvo XC40 is near the end. We can say this because vehicle has been spotted in the streets, and it seems like almost finished. Visually, it reminds on V40 vehicle from same manufacturer. That is most seeable on moldings, roof rails and side skirts. Radiator grille is standard feature of Volvo vehicles, and aggressiveness is boosted with xenon lamps and company's engraved badge. Finally air intake is wide and fog lamps are placed on the bumper. Overall impression is improved with glossy roof made of aluminum and 17 or 18-inch alloy wheels.
The revisions extend to the radiator grille which looks smaller than that of the outgoing model. The grille will also feature chrome edges. Flanking the grille will be a pair of modified Xenon headlights with an option for LED headlights. The front will also feature new fog lights which mounted on the bumper.
Inside the cabin, designers put a lot of effort to improve safety, comfort and infotainment. Seating layout is now different, with more space for passengers. Driver is getting new instrument panel with digital clusters and TFT display. Helping systems are parking assist and cruise control for base models. Safety is improved with laser assisted braking, while blind spot information system is boosted with cross traffic assist.
The seating arrangement has also been altered to create more space for passengers and cargo. Drivers will benefit from the revamped instrument panel which will feature a TFT display and digital clusters. Premium cloth will be the standard upholstery material for low trim models while higher trim vehicles will be upholstered in quality leather.
2017 Volvo XC40 Engine
Base models of the 2017 Volvo XC40 comes packed with 1.6-l gasoline, four-cylinder unit. Finally diesel models will be more interesting, with 2.0-l displacement. This turbocharged powerplant is going to be mated to a 6-speed automatic gearbox and it can produce 150 hp and 220 lb-ft of torque. Petrol engine pumps more power, 180 ponies and 240 lb-ft of torque. Also, different transmission box is going to be offered. The 5-speed manual comes as optional replacement of the 6-speed auto.
The petrol version will be a 1.6 L, 4-cylinder engine which is capable of producing 180 hp @ 5,700 rpm and 240 b-ft of torque between 1600 rpm and 5,000 rpm range. This engine will be paired to a 5-speed manual transmission system as standard and a 6-speed automatic system as an option.
For more power output, buyers can opt for the 2.0 L, 5-cylinder diesel unit that can deliver 148 hp at 3,500 rpm and 220 lb-ft of torque between 1,500 rpm and 2,700 rpm. The engine will be mated to a 6-speed automatic transmission system which will send power to all wheels.
Thank you for reading the article on the Best Auto Release, We hope article about the 2017 Volvo XC40 can be useful as your knowledge in seeing the latest automotive world today.
Image Review Specs 2017 Volvo XC40:
Tags : 2017 Volvo XC40 changes, 2017 Volvo XC40 concept, 2017 Volvo XC40 price, 2017 Volvo XC40 redesign, 2017 Volvo XC40 release, 2017 Volvo XC40 review, design, Engine, Interior, Specs, Volvo, Volvo XC40


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You may need to install Flash or enable it on your browser to play the puzzles.
Can't see the Full Screen puzzle? Your pop-up blocker probably prevented it from opening. Click here to relaunch the pop-up.
Still can't get it to work? Please contact us for additional help
Free puzzle of a sea lion with long whiskers resting his head on a rock. The sea lion just finished swimming in the water.


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Level 2 B-BBEE contributor for the year 2017
Lingua-World is proud to have been named a level 2 B-BBEE contributor for the year 2017,
and we're more inspired and determined than ever to further support the development of the people of South Africa.
For nearly 5 years now, our aim has been to supply the different branches of industry in South Africa with our high-quality services, and we are driven by our task of making mutual understanding possible in all areas of daily life.
Today, translation and interpretation work is a significant part of communication on every level. Thanks to our translations, the people of South Africa read and understand documents, instructions and press releases in the language they call their native tongue, (from a possible 12 languages!).
On the other side of things, translations and interpreters ensure that companies in South Africa can communicate with their business partners in different languages. This leads to increased and stronger relationships as well as improved efficiency, which both mean rapid development for the companies and thus the promotion of economic growth.
BEE's mission is to achieve the distribution of wealth across as broad a spectrum of previously disadvantaged South African society as possible, and to implement measures to erode the huge gap between the rich and the poor, thus creating a dignified standard of living for all.
The success of our work over the last 5 years is not just the professionalism we have gained when interacting with our customers, but most importantly the friendships we have built between Lingua-World (Pty) and the children from Youth Alive Ministries in Dube village, Soweto. The children we have got to know there over the years work on the Lingua-World computers every day and are achieving great results in school. More and more children come every year and gain from our help, developing their skills and chances for a better future.
Through our work, we are sure that we can help improve the lives of even more people!


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Bowling alleys come in many different shapes and sizes. There are countless standalone bowling centers, but they're also common in family entertainment centers. But no matter what kind of bowling center you own, there's good news — we can hook you up with the perfect insurance policy that will ensure you have the best coverage and the lowest premiums.
There are all kinds of accidents that can happen in a bowling center, and plenty of room for property damage. In the case of accidents, you won't regret partnering with Northeast Insurance Center for the best bowling center insurance. As specialists in amusement industry insurance, we know how to meet the unique, niche needs of venues like bowling alleys and entertainment center.
Our specialized programs save an average of 20% to 30% and MORE for bowling centers! Let us put together a program for you today.
Request a Quote
Business Insurance
General Liability
Excess / Umbrella
Equipment Coverage
Workers Compensation
No matter what type of amusement business you own, we can save you money on your insurance premiums.
Call: 800-719-9066
We insure bowling centers and bowling alleys and related amusement venues throughout the entire United States: Alabama, Alaska, Arizona, Arkansas, California, Colorado, Connecticut, Delaware, District of Columbia, Florida, Georgia, Hawaii, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Montana, Nebraska, Nevada, New Hampshire, New Jersey, New Mexico, New York, North Carolina, North Dakota, Ohio, Oklahoma, Oregon, Pennsylvania, Rhode Island, South Carolina, South Dakota, Tennessee, Texas, Utah, Vermont, Virginia, Washington, West Virginia, Wisconsin and Wyoming.


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If you missed it, we posted two new stories on www.locdlife.com last week. Check them out (they're at the top of the list).
Looking for a holiday hair style? Try a loc hawk.
It can be as simple as clipping your hair to the center using bobby pins or decorated clips:
1. Sweep your hair to one side towards the top of your head, leaving a channel for your "hawk". Clip in sections as you go.
2. Sweep up the other side up towards the top in a similar fashion, also applying clips as you go.
3. Continue backward towards the neck.
4. The loc hawk can either hang loose, or be curled with rods.
Or, you can try it by doing a series of buns, as in this video by Chescalocs (http://www.youtube.com/user/chescalocs):
1. Start with parting a triangle section towards the front. Gather, roll into a twist and wrap the twisted locs around the base of that section. Secure with a rubberband. Tuck any loose locs into this rubberband or place a new one to tuck them into.
2. Repeat in small sections, placing the buns at the top and center until you reach the back.
3. You can continue to place the last section in a bun, or gather into a ponytail with a rubberband and hang loose.
Either way, you have a loc hawk—a style that people will remember.


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EDITORIAL: Fall River youth baseball team headed to World Series, looking for community support
On Thursday night July 25 me and my teammates made some history of sorts. We won the New England 8U Babe Ruth / Cal Ripken Regional Championship in Milton, Connecticut. We made it through an incredible week of baseball, in heat that topped out close to 100 degrees, finishing victorious. With the guidance of our coaches and many bottles of water we were undefeated during the entire week of competitive baseball, which included the best 8U teams in all New England. On our road to New England Regional Championship we amassed a 12 and 1 record.
We are now faced with the opportunity to compete at the national level at the World Series in Cherry Hill, New Jersey representing our park Maplewood and our city Fall River, Massachusetts. We love both with all of our hearts. In fact, we play and defeat our opponents with the hard work and heart that are said to be the cornerstone of our hometown. Like our city, our team is comprised of a diverse group of players who share a love of family, our hometown and most of all our love to play baseball together. We grind out every game to the bitter end and "never give up" has become our motto. We also try to live by the Babe Ruth Sportsmanship Code:
Develop a strong, clean, healthy body, mind and soul.
Develop a strong urge for sportsman-like conduct.
Develop understanding of and respect for the RULES.
Develop courage in defeat, tolerance and modesty in victory.
Develop control over emotions and speech.
Develop spirit of cooperation and team play.
Develop into real, true CITIZENS…
We would like nothing more than to end our summer of baseball competing for and earning the World Series Championship. We'd like to show the nation the results of our countless nights of practice in the cages and on the field. From Abbott Park to Maplewood Park, we spend hours in practice and in play culminating in this shot at the national title.
We cannot do this alone and kindly ask for your support. By making a generous donation to our team you are helping us attain our dreams and continue our baseball journey. Our team is extremely grateful that you even took the time to read about us. Your donation will help us with our travel, lodging and dinning expenses as we compete at the Cal Ripken Invitational in Cherry Hill, NJ. On August 7th through August 15, 2019. http://crworldseries.com/. Thank you for your generous support Maplewood Nation!
Thank you!
Joe Charves


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During this webinar, we will look at the top exam issues for 2018 and focus on avoiding the mishaps of poor planning on your examination. This program will move through some of the "hot spots" for BSA this year and help you stay in the clear of traps from the examination process.
Covered Topics:
- Beneficial Ownership on legal entities
- Drillling down on business structures
- High Risk Customers
- New SAR
- New CTR issues
- Risk assessments and risk management
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text: The Sardinia tank maxi dress, is perfect to wear all summer long, made from 100% rayon fabric , allowing your skin to breathe and featuring a flattering V neck, which makes it perfect to add your latest statement necklace to complete your boho summer look. A relaxed fit maxi with pom pom hem detail and useful hidden side pockets. The Sardinia tank maxi dress is the perfect addition to your summer wardrobe, dress it up or down and it will suit all your post Christmas catch ups. Pair it with our Algarve cape to add an extra stylish touch.


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Gwenno has song of the day with 'Tir Ha Mor'
This new track is taken from Gwenno's upcoming 'Le Kov' album, which is written entirely in Cornish. The album will release on the 2nd of March this year. Gwenno has some UK tour dates, starting in Holyhead on the 20th January and visiting Birmingham, Mancheste, Leeds and more. Check here for all dates and tickets.
The last song of the day was Crisis Fest by Sunflower Bean. Listen to all of the songs from 2018 so far in the playlist below!


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If you are still a little undecided as to whether to purchase the new Valve Portal 2 game this video of the first 15 minutes of the new game might help you make the decision.
With mixed reviews and a reportedly short game length in hours of game play and the release of the Potato Sack just before release has sparked a few different thoughts on the new Portal 2 game.
If you didn't already know Portal 2 takes place 100 years after the first one. Portal 2 was released on the PlayStation 3, PC, Mac and Xbox 360 on April 18th 2011.
Have you purchased the new Portal 2 game? What are your thoughts, good or bad?
Source : DVICE


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March 5th
Dear Journal,
Now that I have the majority of my tasks completed for the morning – there will always be more, I know. I am going to take some time to write in my journal, for my own peace of mind, to record some of the things that have happened in my life. Why do you ask? I want to be able to look back on things more readily when I grow too old to actually go out adventuring again.
Let' see, where did I leave off? Ah yes, with my family's permission, I left the forests and our Inn behind me to seek my fortune in the world. Yes, I knew that the training I had had as a barmaid, maid and general housekeeper would work well for me in the cities – I knew that I would find employment out there as I continued my journey.
I did try to find work for a time in Silvermoon, however, that area still feels alien to me even now. There is a lifestyle there that goes beyond anything that I have experienced elsewhere. So many things are socially driven by protocols as well as wealth. Family names are meant to push you further up the social ladder. Since I never was one to give into the social amours, I was still able to find work with a young nobleman and his family.
I spent a pleasant time with this family and enjoyed helping them with their children when the need arose. I do think that they were a bit crazed with having baby after baby, to keep the bloodline going. I realize that our race had been crippled very badly after the Scourge Invasion and we needed to repopulate the area, however, one thing that I did notice with the youngsters is that there wasn't that much time given to them by their parents. The parents were more attuned to the political things and the social gatherings than they were to growing a familial bond with their children. I think that the majority of the young people in Silvermoon today are products of their family's failings.
I stayed with this Silvermoon family for quite a while and the Father was actually one of the high ranking Rangers that was eventually transferred to further his career in Northrend. Ah yes, Northrend, the cold wind blows, the snow falls and there are Scourge to kill as the Rangers went in search of their arch nemesis, Arthas.
Nothing would do for the family other than to make the transitioning to Dalaran to accompany the Father on his journey and to be nearby to offer their support. Now, Dalaran wasn't always the grand city that we know of now, it was through magic and those means that moved it from its earthly place in Azeroth to another – floating high above the rest of humanity.
The city was very beautiful and one couldn't help but be enamored of its charms. So many things going on with the various shops and everything was expensive. Oh, just the beauty and the peace of the city was something that I had never seen outside the confines of Silvermoon City. Well, this city was different, it was a safe haven for all races, the authorities wouldn't allow any clashes or altercations between the factions. You can say what you will, Dalaran was a place where one could easily forget the strife going on in the world.
Several things are of note to write down here in this journal. While this city, Dalaran, was pristine on the surface, there were places where one could have some foul deed befall you and that would be in the Sewers beneath the elegant surface. It was not an area where one would tread without some caution and awareness. I would say that it is, at the very least, comparable to Murders Row in Silvermoon although it is on a much larger scale than that little place could ever hope to achieve.
Life was indeed quite pleasant in that fair city. There was a constant influx of new people on an almost hourly basis, however, if you intended on maintaining a residence in the city, you had to have money. Lots of money. I know the family that I was working for had their wealth established long ago in Silvermoon, however, they chose to take one of the larger residences in Dalaran. Of course, they did have to have room for the tutors for their children that they brought with them, some were left behind in Silvermoon to attend boarding schools.
I found myself putting in some very long hours in this household. I was promoted to Housekeeper and had a staff of six maids and two nannies that worked with me. It was very time consuming and left little time for me to enjoy the fair city and some of the entertainments there, however, I did manage to find some time sit in the park with the nannies and the youngest two children and we would socialize with one another as well as get to know some of the visitors to the city. It wasn't really unpleasant, however, I always had my duties that seemed to never end. Thankfully, I didn't have to interact with the stableman all that often because he seemed to have an aversion to bathing on a regular basis.
Life was still good and the family seemed extremely happy with their lot in life in Dalaran. There were many parties held and many visitors from home to keep things as close to normal as they had been in Silvermoon City. I know that the husband and wife were very much in love and were quite thrilled with the advances that the husband was making in his chosen career path. I guess I don't have to mention that he was next in line to take on the family title, properties and wealth when his Father passed on the next life. It was indeed a happy time.
As it is with all things military, there are certain dangers that go along with the career paths taken by these individuals. One day, late in the Spring, two young rangers approached the door and knocked and asked to see the mistress of the house. I had a sense of foreboding about letting them to see her unannounced and without an appointment, however, they were dressed in their best uniforms and they seemed to be polite. Little did I know at the time that they were bringing notice to the family that the Father had been killed in Ice Crown.
I stayed with the family in Dalaran until all of the funeral arrangements had been made and the body was shipped to Silvermoon for proper internment in the family crypt. I know that I was deeply saddened at his loss because he and I had become very good friends over the time that I worked for them. He had a lilting laughter and pleasant countenance that would have made any young girl fall in love with him. No, I wasn't that foolish, however, I did realize when he died that my feelings were more than that of a friendship.
The family moved back to Silvermoon and I was left behind in Dalaran. I had managed to save quite a bit of my earnings and had decided that I wanted to find employment here. I knew that I might be living a foolish dream and I knew that I only had so much money, so, I definitely had to find work immediately.
I worked as a barmaid for a time at the Filthy Animal – I can understand why the name was so fitting. I know that it was a rough and tumble kind of place and that the clientele there were mostly transient adventurers. The money was good there, the tips were more than I would make in a month as a housekeeper, however, this was not the lifestyle that I wanted to continue on for an indefinite period of time.
One day there was a notice posted on the boards in the Animal as well as near the park where I liked to take my respite from the smoke and noise. It appeared as though someone was looking for a housekeeper to maintain their home in Dalaran. Interesting, they didn't mention the pay, however, they did mention that the house was undergoing some renovations and that the housekeeping would be minimal at first. Didn't mention anything about a family with children, that might be a plus, however, it did mention that the owner was a young, single, independent businessman. It also mentioned that the person was from Silvermoon. The name to contact was Fnor Morningstar – interesting, no title there and that would mean that he was more of the common folk and not any kind of royalty like most of them have in Silvermoon. I decided that I would go and apply for the job.
So, that's where my story and my life got to be interesting.
Agatha Fairsong


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Our Services
A Few Words About Us
Our Philosophy
Our team are a group of friendly individuals with a passion for providing assistance to everyday people, just like us!
Our fresh approach to providing legal services means that we aim to work with you towards your goals. We listen to the outcomes you want and we will deliver our advice in a relatable and easy to understand manner.
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Conflicts are a part of family life. Many things can lead to conflict, such as illness, disability, addiction, job loss, school problems and marital issues. Often times, family conflict becomes too much and you need to explore and learn about new ways to cope.
Our Services
Our experienced EAP counselors are available to assist you in dealing with the many challenges that come with family life. We offer free and confidential consultation, information, and referral services to you and your household members.
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You can call the EAP toll-free, 24 hours a day, 7 days a week at 1-800-322-5327 or email at email@example.com.
Additional Resources:
CDC-Child Development and Positive Parenting Tips
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CDC-Child Development Screening
Ages and Stages-Developmental Milestones
Adventures in Parenting-National Institute of Child Health and Human Development
National Institutes of Health-MedLine Plus-Child Development
Growth and Development-OneToughJob.org
Positive Parenting-Psychology Today
Positive Parenting-Parent/Child Communication
Nine Steps to More Effective Parenting-KidsHealth.org
Child Growth Charts
Child Development Stages and Appropriate Arts Activities
Zero to Three: National Center for Infants, Toddler and Families


text: A delightful middle-school series with a magical touch! Meet Daisy, a baker with a cozy, local bakery and a rotating cast of characters who find their way to her door, each facing a personal challenge. Full of friendship and positive messages, these stories help young readers face their own challenges and remind them that there's always a silver lining to be found.


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Tonight's question, greetings, and banter here. (More)
Today on Meet Sunday State Nation This, Jerry Brown said he isn't sure if Hillary Clinton will be the Democratic nominee and Joe Biden should consider running. Also, Howard Dean called Clinton's responses to Emailgate "too lawyerly" and Michael Mukasey reprised the What Did She Know And When Did She Know It? refrain, while Ellen Tauscher called the email probe "unprecedented" and "partisan politics." Is Emailgate a convenient distraction from GOP WHannabes chasing Donald Trump to strip away birthright citizenship?
Today on Campus
Morning Feature – "What's Wrong With Slavery?" (Ask Ms. Crissie)
Our Earth – Eco News Roundup


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In order to get to where you want to go, you need a map. The sales process is no different—by mapping the steps that your organization takes to lead potential customers along the path of purchase, you'll be more successful in reaching your goals. Here are five valuable tips to help you map your company's sales process.
Maps. There's nothing quite like them for getting us from point A to point B. And when it comes to your company's sales process, mapping may be the most effective way to make sure that each and every step of that process is identified and clarified from beginning to end.
Given that 50% of sales time is wasted on unproductive prospecting, it is imperative that your organization create a detailed sales guide that eliminates inefficiency. Part of your sales strategy should be around mapping out your sales proces.
Through process mapping, your company can better collaborate across all departments to achieve sales goals and objectives. The challenge with sales process mapping lies in doing it correctly, as mistakes can undermine this tool's potential to bring positive business results. That being said, here's a guide to help you map out your company's sales process successfully:
Mapping out a sales process isn't something can be completed in a single afternoon; it's an undertaking that requires the cooperation and collaboration of team members in all departments. Companies with closely aligned sales and marketing functions have 36% higher customer retention rates. As such, before you do anything, you need to make sure that everyone involved is on-board with the idea. For organizations that have yet to embrace more modern systems— meaning that they are focusing primarily on sales results and competition between individuals and departments—the concept of mapping out the entire sales process from end to end will most likely be met with resistance.
Questions regarding what process mapping is, what it involves, and how it will benefit everyone concerned will need to be effectively addressed. In short, you will need to sell your company on the benefits of sales process mapping before you can design or implement an effective map. Otherwise, there will be no point in proceeding to the next step.
Mapping out a journey only works if you know your destination. The same holds true for mapping out the sales process. In this case, your destination is a satisfied, paying customer. Achieving this end result is not dependent on any one department in your organization, but requires all departments—sales, marketing, servicing, etc.—to work cohesively as a team. Mapping out each step of the sales process is vital, from prospecting to closing, and especially to following up (44% of salespeople give up after a single follow-up, yet 80% of all transactions require 5–12 follow-ups before they are successful).
Factoring in the roles that all of your departments play in each of those steps from beginning to end is essential for success.
Without customers, there would be no sales. And yet many organizations map out the sales process journey without considering what the customer is doing along the way. Customers couldn't care less about what's mapped out on your whiteboard. They're on their own Customer Decision Journey, and it's up to you and your sales force to know what that journey looks like.
Once, the old sales funnel assumed a very linear path to purchase, with customers gathering information, narrowing things down, and making the purchase decision. But in today's digital world, a world that is more about customer behaviors, the CDJ is no longer linear and predictable. In fact, it has changed so dramatically that a whopping 68% of B2B organizations have not identified their funnel. That being said, it's critical for your organization to identify and isolate a primary market segment and map out every stage of the journey they are taking on a whiteboard.
Focus your efforts on locating customers where they are, not where you think they should be. Social media and email marketing are both effective and inexpensive ways to contact customers and introduce them to your sales process. In fact, 78% of salespeople who use social media outsell their peers, and email marketing has twice the ROI in average that cold-calling generates. If you realign your focus so that the customer becomes your top priority, your sales force will be empowered with the ability to look at customer actions and behaviors, identify what stage of the buying journey the customer is at, and act accordingly.
For a sales process map to be effective, it is critical that business leaders identify the work steps most closely associated with the stages of the customer journey, and then make sure that those steps and stages are properly aligned from beginning to end. Mapping this out visually will help your salespeople to see where their customers actually are in the journey, as opposed to where they might think they are. This systematic approach to the sales process will help reveal various inconsistencies within the sales force respective to the various stages of the customer journey. But that is a good thing, as one of the main purposes of mapping is to identify weaknesses and strengths within the business process, and bring everyone together to find workable and measurable solutions.
Process mapping doesn't end with a big sigh of relief and a pat on the back as you and your sales force admire the finished map on the whiteboard in the conference room or on a computer screen. As impressive as a process map can look, its real value lies in how it captures and clarifies the realities that an organization has to deal with, and then backs up those realities with evidence and data. However, the realization of that value only comes when an organization actively and consistently adheres to what the map reveals about the sales process and the customer journey. So, trust your map, and put it to work. Otherwise, it's a waste of time.
A traditional map must be followed from start to finish in order to guarantee a safe and timely arrival at the desired destination, and the same rules apply to a sales process map. Getting creative, perhaps by trying a sales tool or technique at a specific stage of the customer journey that the map does not call for is a risky choice, and could end up derailing the sale altogether. However, as people in your organization begin to trust the map and the data, seeing where the problems lie in the sales process and working together to solve them, they'll begin to experience positive results that will help to reinforce that trust.
That's when you (and the teams within your sales organization) will start to realize the real purpose and benefits of sales process mapping. As you stick to the map and learn new and better ways to get things done, each and every step of your sales process will improve, resulting in more sales and greater competitive advantage.


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Not every wish can be fulfilled with the help of your own financial means. This applies in particular to larger purchases, such as new or used cars, as well as expensive household appliances. In such cases, consumer installment loans in claim can take their budget increase . When you take out an installment loan, you quickly receive the required loan amount, with the maximum amount for installment loans being in the five-digit range. The loan amount is repaid in the form of constant monthly installments, which are paid at regular intervals over a certain term.
Make an extensive installment loan comparison
A large number of providers advertise on the market with installment loans on apparently favorable terms. However, not every loan is actually suitable for financing private wishes. For example, there are clear differences in monthly rates and interest rates. Above all, long terms and the resulting high overall interest rates later turn the initially cheap loan into a liquidity trap. It is therefore all the more important that consumers make an installment loan comparison using an installment loan calculator.
Compare online and save time
In the context of such an installment loan comparison, the conditions of different lenders are compared and the cheapest offers are highlighted. The consumer is spared the tedious search for relevant information. The installment loan comparison is also a non-binding service that can be used at no additional cost.
If the installment loan comparison delivers a suitable result, the consumer has the option of applying for the loan at the specified conditions. For this purpose, he transmits his personal data, on the basis of which the responsible credit institution carries out a credit check. The applicant's creditworthiness in turn serves as the basis for the decision on whether to grant the loan amount. This does not apply to loans that are granted without a previous request to a credit agency.
Check debt rescheduling
Existing loans should also be checked from time to time with an installment loan comparison for possible savings. In periods of low interest rates, it can be worth terminating existing contracts and converting them into a cheap installment loan. Many banks can flexibly replace old contracts. It is important to compare here. Whether rescheduling is possible depends on the existing contractual conditions.
Credit Amount: USD Loan term: 1 month 2 months 3 months 6 months 12 months 24 Months 36 months 48 months 60 months 72 months 84 months 96 months 108 months 120 months Usage: Free use New cars / used cars Furniture Modernization / renovation vacation PC / Multimedia credit repayment Balance current account to compare


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Memory on Snowfield is a North suit that appeared in the Flowing Garden Event.
Wardrobe Edit
- Hair: Falling Snow Reflex
- Coat: Striped Shawl
- Top: Honor Top
- Bottom: Ideal Culottes
- Hosiery: Cashmere Stockings
- Shoes: Ankle Boots of Wish
- Hair Ornament: Twisting Curve
- Earrings: Lonely Fillet
- Brooch: Stamp of Youth
- Hair: Falling Snow Reflex-Clear
- Coat: Striped Shawl-Clear
- Top: Honor Top-Clear
- Bottom: Ideal Culotte-Clear
- Hosiery: Cashmere Stockings-Clear
- Shoes: Ankle Boots of Wish-Clear
- Hair Ornament: Twisting Curve-Clear
- Earrings: Lonely Fillet-Clear
- Brooch: Stamp of Youth-Clear
It's a snowy day when Vicky left her hometown. The snow buried her home and family. She had to step on the journey.
– Falling Snow Reflex
In the night under a cliff, Leia sang an old home song. It echoed in Vicky's deep memory.
– Striped Shawl
The snow goes bigger and bigger in the wildness, but Leia's song is like the spring wind, taking Vicky's pain away.
– Honor Top
Leia's feelings radiated through her song. Vicky felt that she had to help Leia find her love.
– Ideal Culottes
Vicky heard that Leia was once a singer in the troupe. She never left North, so she couldn't imagine it.
– Cashmere Stockings
In December, the cold dragged the war into a deadlock. Vicky's troop moved to the north line. They are further away
– Ankle Boots of Wish
Trapped in heavy snow, two troops met. It's the first time when Vicky and the medic Leia met.
– Twisting Curve
When Vicky became a military messenger, she left the frontline. Sometimes, she would wake up in the cold night.
– Lonely Fillet
Vicky told Leia that when spring comes, she will return with the message of the young man. Leia sang her goodbye.
– Stamp of Youth
The train took Vicky to the frontline. Though she had her own tasks, she never forgot Leia's wish.
– Falling Snow Reflex-Clear
Vicky chose to tell Leia that the young man went to the frontline when they met again. She had seen too much misery
– Striped Shawl-Clear
This is the coldest winter since Vicky left hometown. No one would sing the familiar song by her side anymore.
– Honor Top-Clear
When they met again, Vicky told Leia that she had found the man, but Leia begged her to not say it.
– Ideal Culotte-Clear
But Vicky didn't give up. Leia's love supported her on the tundra and mountain. She had to take the message back.
– Cashmere Stockings-Clear
The short passion was long gone. Leia's memory of the young man is more about affection toward hometown and youth.
– Ankle Boots of Wish-Clear
Hearing Vicky's words, the young man didn't say a word. Leia's dream never exists. It's a joke of the wind.
– Twisting Curve-Clear
Asking around, Vicky found the young man that Leia admires. She asked him if he remembered that silver-haired girl.
– Lonely Fillet-Clear
Meeting again after the long separation, Leia and Vicky wept and hugged with one another. Finally, spring will come
– Stamp of Youth-Clear
Interpretation Edit
Coming soon...
- See also: Handbell in the Wind.
Name by ServerEdit
|Name (Romanized)
|International (English)
|Memory on Snowfield
|International (French)
|Mémoire du Champ de neige
|Snowfield Memory
|China (Simplified)
|雪原的思念 (xuěyuán de sīniàn)
|Snowfield NostalgiaCT
Taiwan/Hong Kong/Macau (Traditional)
|雪原的思念 (xuěyuán de sīniàn)
|Snowfield NostalgiaCT
|雪原の想い (Setsugen no Omoi)
|Snowfield Sentiment
|South Korea
|설원의 그리움 (seol-won-ui geulium)
|Snowy LongingCT
|Kerinduan atas Padang Salju
|Longing for the Snow Fields
|Nỗi Nhớ Đồi Tuyết
|Snow Hill Nostalgia
|Southeast Asia (English)
|Snowfield Memory
Gallery Edit


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Hey guys, Bulba here and a common discussion has been going on on my Twitter recently. Who will it be, Steve or Banjo that gets in as the Microsoft rep for Smash DLC? Well, that is what I'm here to discuss! Also, keep in mind Vergeben (the most credible Smash leaker) only said we would get Minecraft content and is not sure if we will get Steve for DLC. Anyways, let's get to it!
Let us begin with Steve. Without a doubt Minecraft has made a huge mark on the entire gaming industry, going on to still sell after millions of copies already sold. It has even released on Wii U, (New) 3DS, and Nintendo Switch. Speaking of the Nintendo Switch version, Minecraft on there received the "better together" update where crossplay was pushed very heavily with Xbox. What is the point of all of this? Well, Nintendo and Xbox have been very friendly towards each other while discussing Minecraft, saying they have been encouraging each other for crossplay. This does very well help Steve's chances as Nintendo has just finished choosing the DLC for Smash. There is not too much else going for Steve, but the fact that he is part of one of the top three best selling games is enough to get him in Smash.
Before moving on to Banjo, I wanted to discuss Steve's moveset and a potential problem for him. His moveset could consist of his sword, hands, bow and arrow, ender pearl and potions. However, the problem is how he would perform his moveset. Steve has very limited movement, meaning it would be awkward to see him hang on to ledges, jump, and attack without bending his limbs. Also, Sakurai has stated he spent a lot of his time focusing on character expressions, while Steve would have the same pixely face which never changes. Finally, Sakurai has stated he likes Minecraft but that does not mean much as he also stated he played Shadow the Hedgehog.
Next is Banjo, who I personally want more. One of the biggest evidences for Banjo is that he (in an unofficial Smash Ballot for Japan) was the number one most wanted third party, even surpassing Geno and Shadow. Perhaps Microsoft wants to make their relationship with Nintendo go beyond Minecraft, or vice versa and Banjo in Smash would be the perfect way to do so. Thirdly, he has the history with Nintendo as he originally was a Nintendo IP first appearing on the Nintendo 64. Other than that, Banjo does not have too much evidence, but history has shown with Mega Man in Smash 4 that fan popularity can be more important than relevancy.
Next up is the evidence against Banjo, and although I said fan popularity > relevancy, Banjo has not had a game in a decade while it had only been one year or two since Mega Man's previous game before the Smash 4 roster was chosen. Another potential problem is that Nintendo would also have to go through their old buddies back at Rare, who may or may not be willing to put Banjo in. Finally, it is important to remember Nintendo has chosen the DLC meaning they would likely pick a character from the second best selling game of all time (Minecraft) over Banjo.
So, who will it be? Banjo or Steve? I think the most likely scenario would be Banjo making it in along with a Minecraft stage/assist, as it would eliminate the problem of Steve's movement issues while still giving his series representation. Also, I'm sure by now Nintendo knows Banjo could make them even more money than Steve as his fan support is all around the world, and not mainly in one region. It is also worth noting that Banjo is a more established character, as Steve is only the main skin used to promote Minecraft even though most people change their skins to be different people. Finally, it would go along with Vergeben who is still saying he is confident in Minecraft content but not a fighter.
Thank you so much for reading! Who will it be, Banjo or Steve? Let me know in the comments below, on Twitter or on Discord. I also want to thank you for voting in my poll on Twitter, where my followers overall thought Banjo was more likely (you can see above). See you soon!
(Image credit: Banjo and Steve are both properties from Banjo- Kazooie and Minecraft, both of which are owned by Microsoft. Edited by me [BulbaSour])


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Episode 020 – Lies, Exaggerations, and Other BS
(Note: This document contains the essential content of this episode but is not necessarily a complete verbatim transcript. It may also contain bonus content not included in the episode.)
= = =
Amidst the noise and confusion generated by all the speakers, authors, coaches, infomarketers and other assorted gurus who are screaming at you about how simple and easy it is to become phenomenally successful in business with little or no effort on your part if you'll just buy their book or course or coaching program, you'll occasionally find someone who is willing to tell you the truth about business and entrepreneurship.
I'm one of those people.
But before proceeding I want to say that if you don't want to hear the truth about entrepreneurship, then tune out right now and be on your way, and I wish you the best, but… If you think you can handle the truth, then stay with me.
With respect to figuring out how to make your entrepreneurial adventure be the best it can be, you basically have 2 choices:
- You can allow yourself to get sucked into all the lies, exaggerations, and feel-good fantasies that are tossed your way every single day, OR…
- You can learn to recognize lies, exaggerations, and feel-good fantasies and ignore them in favor of rational thought.
You can learn that business, and everything else in your life, is an experiment.
You can learn that some experiments produce good results, and some produce bad results, and that you cannot know in advance which it will be.
You can learn that what someone else has done or accomplished has little or nothing to do with you.
You can learn that methods, strategies and tactics that someone else has used have little or nothing to do with you.
You can learn that if you were to read all the books there are, watch all the videos there are, take all the courses there are, and spend thousands of dollars on coaches and consultants, you would still have to figure out, on your own, by yourself, how to make your adventure work, because what will work best for you will be unique to you.
Once you've learned these kinds of things, you can…
- Stop looking for canned answers to your questions and one-size-fits-all solutions to your problems.
- Start looking for ideas from which you can create your own answers and solutions because those are the answers and solutions that will work best for you.
= = =
Imagine if someone said:
"I want to become a very successful entrepreneur, so I'm looking for someone who can tell me exactly what I should do to accomplish that."
No one can do that for you.
You must find your own way, your own formulas.
You must experiment to find out what works well for you.
= = =
Consider the following quote:
"There's a science to success, and it's quite simple: Model those who are already successful, gain their specialized knowledge and take action."
~ Tony Robbins
This statement is false, or, at the very least, a gross over-simplification.
Stop buying into the notions that:
- They did it, so I can do it too.
- They are successful, therefore if I just do what they did, I will be successful too.
- You are unique.
- Your circumstances are unique.
- You can get ideas from what others have done, and you can experiment with those ideas, but you cannot simply copy what they've done and expect it to work the same for you.
= = =
Failure Is Not an Option!
- This notion is delusional and irrational.
- Failure is ALWAYS an option.
- People don't get married believing they'll end up getting divorced. But it happens.
- People don't start businesses believing they'll end up bankrupt and out of business. But it happens.
- Start with acceptance of the idea that you might fail, but that you're going to do everything in your power to prevent it.
= = =
More ridiculous statements and notions:
- It's never too late…
- He did it, and if he can do it, anybody can do it.
- I did Action A and got Result B, therefore if you do Action A, you'll get Result B.
- Launch a (website/blog/podcast/YouTube channel) and millions of people will be able to see it/read it/hear it/watch it! (clue: millions of people will be able to see it/read it/hear it/watch it, but that does not mean they will see it/read it/hear it/watch it.)
= = =
Are these statements true?
- You can BE ANYTHING you want.
- You can DO ANYTHING you want.
- You can HAVE ANYTHING you want.
No, they are false.
Are these statements true?
- You can BE MORE than you are now.
- You can DO MORE than you are now doing.
- You can HAVE MORE than you now have.
Yes, they are true.
= = =
False and/or Misleading Phrases:
- It's simple…
- It's quick and easy…
- It's effortless…
- All you have to do is…
- We've cracked the code…
- We'll pull back the curtain…
- You can become unstoppable!
- Just X easy steps to achieve…
- This is the ultimate guide to…
- You'll learn the secrets of success!
- We will unveil the hidden formulas for…
- This program will unleash your superpowers!
= = =
Ridiculous Claims:
30 to 50 new high-ticket clients each month!
How to Create a Stunning Ebook Online in 30 seconds Without Writing a Single Word!
Master the Inner Game of Business in 2 Days!
Learn HTML 5 in 1 hour!
= = =
Consider this quote:
"Sadly, many parents, educators, and do-gooders have meddled with the natural development process of curious, exploring children. Realizing the power of confidence, many well-meaning people have attempted to simply give it, unearned, to children by constantly telling them they can do anything. Not only is this untrue, and obviously so to the child, but it has the complete opposite of the intended effect. Children hear phony self-esteem platitudes about achieving their dreams, while they struggle to hit the ball or beat the game, and they assume the world is a sham and that fate is the determining factor of their success or failure."
~ Isaac Morehouse, founder of Praxis, from his article "The Future of School"
= = =
You are Unlimited!
- This is a delusional, irrational, and false statement.
- You are NOT unlimited!
- You have limitations.
- Lots of them.
- All kinds of them.
- So stop trying to convince yourself that you're unlimited and start figuring out what your limits are so you can deal with them.
Here's one of my very favorite absurd claims:
"I'll teach you how to make over $50,000 per month, working only 4 hours per week, and you don't need:
- any experience in marketing
- any experience in selling
- a product
- a website
- an online store
- a blog
- an email list
Sign up now for my webinar on (date/time).
Don't miss this amazing opportunity!
= = =
Learn to tell the difference between rational, realistic claims and BS.
And decide that you're not going to use any of these ridiculous tactics in your own marketing.
= = =
Upcoming Episodes:
Episode 021 will begin "Managing Your Self", a series of 8 episodes where we explore how the human psyche works, and how understanding that can be the deciding difference in how you and your business function.
The episodes are:
- 021 - If You Can't Manage Your Self…
- 022 - The Human Condition
- 023 - Mindset – What Makes You You
- 024 - The Human Psyche
- 025 - Ego: the Enemy
- 026 - Resistance: the Enemy's Assistant
- 027 - Outsmarting the Enemy
- 028 - Love and Fear
Think for yourself. If you don't think for yourself, then other people will do your thinking for you, and that's not a good thing.
Read books. Read blogs. Watch videos. Watch TED Talks. Listen to podcasts. Learn something new every single day. Never stop learning. You cannot foretell when a piece of knowledge will become useful to you.
Create something -- something good -- something that wasn't there before and would never have been there if you hadn't shown up.
Think - Learn - Create
Be Brave - Be Bold
This podcast is also available on these platforms:
Copyright 2019 by Tom Harris


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Latest posts by Robijyoti Bhattacharjee (see all)
- What when animals take control of the human world. An Artist Tries To Paint That Idea into Amazing Artworks - December 12, 2014
- After storming Twitter MufflerMan Trends on Touchtalent - December 9, 2014
- A Native Artist Frames Philippine's Mayon Volcano In Different Angles. The Collection is So Awesome! - December 3, 2014


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Baroness Lawrence was pleased to be invited to Dunraven School in southwest London to attend a special assembly led by a group of sixth form students in the run-up to the first annual Stephen Lawrence Day.
Drawing on the educational resources provided to all schools in England by the Stephen Lawrence Charitable Trust (which you can also find here), the Year 12 students ran their own assembly on the theme of 'Live our best life', which they presented to Year 7 classes, the Baroness and her son Stuart. This was followed by a Q&A session, which gave the children a chance to ask her any questions the assembly had raised.
The Baroness has always maintained her belief that "the lessons of fairness and respect must start early if we are to have a truly fair and civilised society, free from discrimination where we all play our part as active citizens", and felt that the students had done a wonderful job of conveying the importance of making positive choices and respecting one another to their younger audience.
She was delighted that they had responded so positively to Stephen's story, undertaking their own research about how to live their best lives and what this truly meant to them, and enjoyed meeting them and learning about their future plans.
The school assembly at Dunraven will be repeated at schools up and down the country over the next weeks and months as people remember Stephen and feel empowered to make a difference in their schools and communities.


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Here are some photos I have taken over the last week.
I love to thrift and found this divided basket at Goodwill last week for $3.99. I'm not sure if I will sell it or keep it. We haven't been entertaining too much but this would be great for different pretzels & chips. It is on a lazy Susan which is pretty great.
A couple in our church had a baby girl. I sent the present in the mail since I knew they wouldn't be at church for a few weeks. Her name is Harper. I stock up on Kraft paper from the Dollar Tree since you can use it for almost any gift. Love it!
Isn't the human body amazing? I am starting to bruise from my IV and I love to see the different colors.
Spring cleaning time! Both the hubby and I love to organize. I realize we are weird but we both look forward to it. On Saturday we did our annual "clean out the file cabinet" where we go through every. single. file and see if we can throw anything out. We wound up with a big trash bag full of paper.
I love having a window across from my bed. It allows me to see the wonderful sunshine! We went from a -20 wind chill to 50 degrees in one day last week.
My hair is getting SO long. I have trimmed it myself but really need a trip to the hair dresser. One of the symptoms of RSD is hair growth.
Hair dye from eSalon.com. I will be doing a post about this next week. So much cheaper than the salon!
I also found a pair of shorts at Goodwill last week. I cannot wear pants too often since the material presses against my knee. This is a common outfit for me - a bleach stained GAP hoodie, my husband's oversized t-shirt and shorts. It is a good thing we don't entertain too much!
A few years ago we painted "my" room (i.e. the guest bedroom) and we installed new shelving in the closet. We removed the doors and put up the curtains. For about $120 I had a new closet. I wish I would have remembered to take a "before" photo.
|Do you see the box sitting on the shelf? That is my father-in-law's medicine.
I put it together every week. I wish we had another spot for this in the house but for right now it is in here.
Tucked behind the curtain are my belts, umbrella & purses. I'm down to two!
Please continue to pray for my knee. The injection hasn't helped at all. The doctor told me not to be discouraged since there are other things to try. I will go back to the doctor who did the injection in about 10 days to see what is next. Until then I am thankful for my cane and my new (thrifted!) wheelchair.
I hope everyone has a great week!


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With Community Education, there truly is something for everyone at DTM. In our Parent and Child Together (PACT) activities, we have had hundreds of families participate beginning with our Muffins with Mom event in September. This event featured a book reading and signing by our current school board member, Ms. Danielle Green. 180+ mothers and students filled the auditorium doors to listen to Ms. Green's story of becoming an author, as well as reading and signing her book "Hey Pretty Girl". October's PACT event was Donuts with Dad, which over 120+ children along with their fathers gathered to eat donuts and drink apple cider while reading books together at the table. This event also featured guests from Fathers and Men Engaged to speak about the positive benefits of organizing a male role model group here at DTM of which 11 dads signed up to be at the forefront of this effort here at DTM.
We have hosted various family nights with school partners which included a 2 and ½ day blitz in yoga and mindfulness with Holistic Life Foundation. This visit was highlighted by a yoga and breathing lesson in every classroom, as well as a mindfulness lesson for the Courtyard party on September 11th. We have also hosted a Fall into reading Family night on October 22nd which also included activities to highlight advocacy for Lights on After School week in connection with the After School Alliance. Over 110 families attended this event.
In partnership with Rivers of Living Waters Ministries, we hosted a Harvest Celebration that included free food, games, candy bags and a game show for parents on October 27th. The event was attended by well over 250 parents and children.
In adult physical education, instructor Lynne Peterson has grown the B-Fit aerobic hustle class to over 95 unique participants, with an average of 45 per week since beginning September of 2018. Participants range in age and gender, which is truly something for everyone.
Contact Chris Collins, Community School Director at DTM, to get connected: email@example.com.


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+8 8 0
It's hot enough in China to fry an egg on the street
"It's hot enough to fry an egg" seems to be a universal expression of how damn hot it is. Most of the time, though, it's not really that hot. An egg must be heated to 158 °F (70 °C) before it properly fries. But the Associated Press reports that this child in China's Shandong province was...Continue Reading http://qz.com
Join the Discussion
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Ill take an order of egg fried rice with a side of shrimp please!
On a serious note, stay in the shade and keep hydrated. Hope it passes quickly.
Holy shit that is hot. At least they are cooking it on a pan and not the pavement like certain people at a national park. You know who you are!7 years agoReplyLink Save2 Replies
If you're wondering what 99bottles is referring to:7 years agoReplyLink Save1 Replies


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L. Gregory Appelbaum1,2, Julia E. Schroeder1,3, Matthew S. Cain1,3 and Stephen R. Mitroff1,3* Improved Visual Cognition Through Stroboscopic Training (click PDF in the right column to download full paper)
Borch-Jacobsen, Mikkel. Lacan: The Absolute Master. trans. Douglas Brick, Stanford University Press 1991. (In Place of an Introduction)
Lenoir, Timothy. "The Eye as Mathematician: Clinical Practice, Instrumentation and Helmholtz's Construction of an Empiricist Theory of Vision"
Lenoir, Timothy. "Helmholtz and the Materialities of Communication"
Lenoir, Timothy. "Operationalizing Kant—Manifolds,Models, and Mathematics in Helmholtz's Theories of Perception"
Lenoir, Timothy. "The Politics of Vision: Optics, Painting, and Ideology in Germany, 1845-95"
- Plates (images) to accompany Lenoir "The Politics of Vision" WARNING: File is very large, ~19MB
Sawyer Seminar Thematic Bibliography (partially annotated) – Read-only Google Doc in progress and updated periodically


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Monday, 5-Feb-2018 at 6:00 PM--RootsMagic-4, Summers, Local History Room, Largo Library.
Tuesday, 6-Feb-2018 at 6:00 PM--Family Tree Maker 2017 Basics--Part 5, Hahn, Local History Room, Largo Library.
Thursday, 8-Feb-2018 at 10:30 AM--INTRODUCTION TO GENEALOGY(Beginner) -- Basics of how to get started in researching your family history—what to do first, where to look & how to do it. Bryan, Local History Room, Largo Library.
Friday, 9-Feb-2018 at 10:15 AM--Franco-American Discussion Group.Geographic areas include: Europe (primarily France, but not limited to France)-Eastern Canada (primarily Quebec, some Ontario, but including the Acadians of the Maritime Provinces)-USA (Central North America and New England, also Louisiana and the surrounding Southern states). Charest-LeFebvre, Local History Room, Largo Library.


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November 4, 2017
By Lisa Jevens
This story was originally published in the Chicago Tribune Prime Time senior living section.
Whether it's Veterans Day or any day, our veterans always deserve something special that lets them know we remembered. Here are some gift ideas to show your favorite veteran how much you value his or her service.
1. Military...
This story was originally published in the Chicago Tribune Prime Time senior living section.
Visitors to the nation's capital might wonder why there is no museum memorializing World War II. That's because it's located in New Orleans. The National WWII museum claims to be the No. 1 attraction there and the second-h...
October 14, 2017
If you have been shopping around for an independent living community lately, you have probably noticed that many are transforming their dining programs.
They are moving away from traditional dining room meal-a-day plans to...
There are so many convenient ways to listen to music, at home and on the go. You'll be happy to know that the technology is getting simpler and less expensive too. Here are some gadgets that will allow you to play your musi...
September 16, 2017
How much would you pay to never lose your keys again? What about your phone? Misplacing things cost money, wastes time and is just plain maddening. Fortunately, GPS, Wi-Fi and cellular technology allow us to electronically...
Alexa, what time is it?
Maybe it's time to get a voice-activated assistant like the Amazon Echo or Google Home.
These hands-free, voice-activated, internet-connected speakers are as appealing to the Greatest Generation as t...
August 26, 2017
Long before it became trendy to retire to a college town for the culture, activities and intergenerational milieu, retirees have been attracted to Evanston.
For decades, the picturesque lakefront city at Chicago's northern...
Dogs and cats require and deserve a lot of loving care from you, their human parents. Fortunately you can do a lot of this at home with the many specialized products designed to maintain a pet's health and grooming regimen....
June 17, 2017
In the age of digital cameras and smartphones, does anyone print their photos anymore? They sure do, and these new-style printers are part of the reason why.
1. Kodak Dock & Wi-Fi 4 x 6 Photo Printer
This highly-rated photo...
The guidelines for when and how often to get common cancer screening tests such as colonoscopies, PAP smears, PSA tests and mammograms have changed over the last few years. It's important to talk to your doctor about when...
Older Posts >
Branded Content for Studio 1847, part of the Chicago Tribune.


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Anti-LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
Key features and details
- Mouse monoclonal [H4B4] to LAMP2 - Lysosome Marker
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: African green monkey
- Isotype: IgG1
Product nameAnti-LAMP2 antibody [H4B4] - Lysosome Marker
See all LAMP2 primary antibodies
DescriptionMouse monoclonal [H4B4] to LAMP2 - Lysosome Marker
Host speciesMouse
SpecificityHuman CD107b/LAMP-2
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: African green monkey
Does not react with: Mouse, Rat
The details of the immunogen for this antibody are not available.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline
Concentration information loading...
PurityAffinity purified
Clone numberH4B4
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
Alternative Versions
Compatible Secondaries
Isotype control
Recombinant Protein
Related Products
Our Abpromise guarantee covers the use of ab25631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt
|Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Use at an assay dependent concentration.
In our hands milk blocking is gives superior results to BSA blocking for this product.
|Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
Sequence similaritiesBelongs to the LAMP family.
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
- Information by UniProt
FormAlternative splicing produces 3 isoforms.
Alternative names
see all
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
All lanes : Anti-LAMP2 antibody [H4B4] - Lysosome Marker (ab25631) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human liver lysate
Lane 4 : Human liver membrane fraction lysate
Lane 5 : Human skeletal muscle lysate
Lane 6 : Human brain lysate
Lane 7 : Human kidney lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Performed under reducing conditions.
Additional bands at: 100 kDa (possible post-translational modification), 110 kDa (possible post-translational modification), 120 kDa (possible post-translational modification)
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. After transfer, the membrane was blocked for an hour in 3% milk before being incubated overnight at 4°C with mouse monoclonal [H4B4] to LAMP2 (ab25631; diluted 1:5000). Antibody binding was detected using peroxidase labelled goat anti-mouse IgG (ab97040; diluted 1:50000) for an hour at room temperature and visualised using ECL development solution.
Ab25631 staining human normal placenta. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Overlay histogram showing THP1 cells stained with ab25631 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25631, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
All lanes : Anti-LAMP2 antibody [H4B4] - Lysosome Marker (ab25631) at 1/500 dilution
Lane 1 : HEK293 cell lysate at 30 µg
Lane 2 : HepG2 at 30 µg
Lane 3 : THP-1 at 30 µg
Lane 4 : 3T3-L1 at 30 µl
Lane 5 : Mouse hepatocytes at 30 µl
Lane 6 : Rat hepatocytes at 30 µl
All lanes : HRP conjugated Goat anti-Mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 30 seconds
Along with THP-1 macrophages, other human cell lines were loaded, and the 110 kDa mature band for LAMP2 was detected in all the samples. On the other hand, mouse and rat cells were negative. The antibody works really good on human samples, detecting a single 110 kDa band but it's not suitable to use for mouse or rat samples.
Anti-LAMP2 antibody [H4B4] - Lysosome Marker (ab25631) at 1/500 dilution + whole cell lysate prepared from THP-1 macrophages at 30 µg
Goat anti-mouse IgG conjugated to HRP at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Primary antibody incubated for 12 hours at 4°C.Gel running conditions: 12%Blocked with 5% milk for 1 hour at 25°C.
Western blot analysis of Rhesus monkey primary retinal pigmented epthelium whole cell lysate and HeLa cell whole cell lysate (20µg/lane) labelling LAMP2 with ab25631 at 1/1000. An alkaline phosphatase-conjugated rabbit anti-mouse IgG was used as the secondary antibody.
Flow Cytometry analysis of Human T lymphocyte cell line Jurkat labeling LAMP2 with ab25631 at 1 μg/106 cells dilution (purple). A Goat Anti-Mouse IgG1, Human ads-FITC was used as the secondary antibody. Grey - Isotype Control, Mouse IgG1-UNLB, followed by Goat Anti-Mouse IgG1, Human ads-FITC.
References (124)
ab25631 has been referenced in 124 publications.
- Odle RI et al. An mTORC1-to-CDK1 Switch Maintains Autophagy Suppression during Mitosis. Mol Cell 77:228-240.e7 (2020). PubMed: 31733992
- Johnstone DL et al. PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights. Brain 142:542-559 (2019). PubMed: 30668673
- Liu Y et al. Prevalence and clinical characteristics of Danon disease among patients with left ventricular hypertrophy and concomitant electrocardiographic preexcitation. Mol Genet Genomic Med 7:e638 (2019). PubMed: 30929317
- Peña E et al. Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer. Free Radic Biol Med 135:283-292 (2019). PubMed: 30902760
- Kulshrestha A et al. Targeting V-ATPase Isoform Restores Cisplatin Activity in Resistant Ovarian Cancer: Inhibition of Autophagy, Endosome Function, and ERK/MEK Pathway. J Oncol 2019:2343876 (2019). PubMed: 31057611


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The MS system, as explained previously, uses a cardioid microphone to pick
up an all-round mono signal, and a figure-of-eight mic to pick up the difference
between left and right in the sound field. The M and S signals can be combined
without too much difficulty to provide conventional left and right signals.
This is of practical benefit when it is necessary to record a single performer
in stereo. With a coincident crossed pair, one microphone would be pointing
to the left of the performer, the other would be pointing to the right. It just
seems wrong not to point a microphone directly at the performer, and with the
MS system you do, getting the best possible sound quality from the mic. It is
sometimes proposed as an advantage of MS than it is possible to control the
width of the stereo image by adjusting the level of the S signal. This is exactly
the same as adjusting the width by turning the mixing console's panpots
for the left and right signals closer to the centre. Therefore it is in reality
no advantage at all.
The MS system, as explained previously, uses a cardioid microphone to pick


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July 7, 2011
Engl024/Essay 6
Regarding my Education
My education is very important to me; I would like to be more effective. At this time
Iam in my second semester in college. I have done a little research in the Medical assistant field, there is no formal education training requirement. I feel me having
AA degree will enchance my application. But to do more in the medical office such as takingX-rays, giving injections does require taking the course, in the furture I will pursue my education in office manager in the heatlhcare.
I have not yet taking medical terminology, mathematics, or keyboarding and transcription. I already have the keyboarding experience. At this time I do have some qualifications in this field, dealing with the public. Also like the constantly interact with other people. This is a technology world we live in today but medical field is a good field to be in today.
For me to be successful in my education I need to work hardner in my studies and stay focus. Even in this second semester I having hard time but I am getting it together as long as I stay on the right track. As far as me being able to listen in class what has be taught and retain it in my mind, Iam sometimes not able to recollect. I need to concentrate more.Would like to do some volunteer work at the hospital or nursing home to have something under my belt and it will enhance my application.
Education is good for any one at any age it is never to late to