text:
From OpenWetWare
(→References) |
Line 1: | Line 1: | ||
[[Category:CH391L_S13]] | [[Category:CH391L_S13]] | ||
- | DNA synthesis and molecular cloning are tools used by synthetic biologists to create the biological "parts" needed to design and engineer biological devices and systems. | + | DNA synthesis and molecular cloning are tools used by synthetic biologists to create the biological "parts" needed to design and engineer biological devices and systems. DNA comes from somewhere. Be it from another organism or from a tube of chemicals. Nucleotide bases can be assembled one at a time to form longer oligonucleotides. Oligonucleotides are single-stranded pieces of DNA. The other strand of DNA can be synthesized de novo or from the existing strand using DNA polymerase. |
+ | |||
+ | The resulting double stranded pieces are then put together to build larger fragments of DNA. As the length increases, we make a distinction from oligonucleotide (~200 bases) to gene (~1000 bases or 1 Kb), and then from gene to genome (~1,000,000 bases or 1 Mb). | ||
+ | |||
+ | The methods used to manipulate DNA from a single nucleotide to one million bases are discussed here. | ||
==Introduction== | ==Introduction== |
Revision as of 19:21, 4 February 2013
DNA synthesis and molecular cloning are tools used by synthetic biologists to create the biological "parts" needed to design and engineer biological devices and systems. DNA comes from somewhere. Be it from another organism or from a tube of chemicals. Nucleotide bases can be assembled one at a time to form longer oligonucleotides. Oligonucleotides are single-stranded pieces of DNA. The other strand of DNA can be synthesized de novo or from the existing strand using DNA polymerase.
The resulting double stranded pieces are then put together to build larger fragments of DNA. As the length increases, we make a distinction from oligonucleotide (~200 bases) to gene (~1000 bases or 1 Kb), and then from gene to genome (~1,000,000 bases or 1 Mb).
The methods used to manipulate DNA from a single nucleotide to one million bases are discussed here.
Contents |
As described before, synthetic biology captures a diverse, multi-disciplinary field. No matter which definition(s) becomes accepted, the ability to make and manipulate DNA is a vital component to practicing synthetic biology.
A large number of parts have been made by the synthetic biology community. Many can be found as part of the Registry of Standard Biological Parts. These modular genetic components are designed to be easy to acquire and assemble to facilitate the building of more complex biological devices. To learn more about the Registry and the biological parts known as BioBricks™, see the entry for the iGEM Registry.
The Registry of Standard Biological Parts is an attempt to create an annotated and characterized repository of biological parts. It is motivated in part because synthetic biologists rely on the ability to make testable biological units. While the parts registry is a useful resource, it is not comprehensive. The ability to manipulate and create genetic material is a necessary skill for being a successful synthetic biologists. This page details how to create DNA from small (<60 nts) oligonucleotides to larger genes (~400 nts) to genome sized (~500 ,000 nts) biological units. Many of the methods found here are the basis for the construction of the registry itself.
DNA Synthesis
Oligonucleotide Synthesis
Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.
Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
Gene Synthesis
Gene synthesis, or artificial gene synthesis, refers to the process of creating a nucleic acid template for a gene in vitro, without the requirement of a preexisting DNA template. Soon after the elucidation of the genetic code and the description of the central dogma of molecular biology, there arose a need to synthesize genes de novo in order to study their biological function both in the test tube and in model organisms. Chemical synthesis of DNA has grown from an expensive and time-consuming process into a viable commercial industry capable of high-throughput manufacture of almost any scale of custom DNA molecules in almost any context. This allows species-specific gene optimization, creation of genes from rare or dangerous sources, and combinatorial assembly of any DNA sequence that can be chemically synthesized, even including non-traditional bases. The most advanced applications of gene synthesis have been applied to the recent creation of completely synthetic minimal genomes in prokaryotes.
Despite nearly four decades of progress in gene synthesis technologies, most DNA sequences used in modern molecular biology are assembled in part or in whole from naturally occurring templates. However this limits the scope and applications to previously existing genes and the results of large-scale genomic surveys of novel genes from nature. Modern gene synthesis relies heavily on advancements in chemical DNA oligonucleotide synthesis, with the primary challenges being scale, cost, fidelity and the eventual assembly of complete gene products.
An extensive, but not comprehensive, directory of commercial gene synthesis providers can be found at Genespace.
Gene synthesis is one of the key enabling technologies of synthetic biology. The increase in efficiency and the decrease in cost is occurring at a staggering rate. Below is a graph comparing DNA synthesis to Moore's Law. In addition, it includes another enabling technology, DNA sequencing.
While genome synthesis is technically feasible, it is not yet a commercial service. Below is a figure describing the cost of genome sequencing.
History of Gene Synthesis
Gene synthesis predates the invention of restriction enzymes and molecular cloning techniques by several years. The first gene to be completely synthesized in vitro was a 77-nt alanine transfer RNA by the laboratory of Har Gobind Khorana in 1972 [1]. This was the result of nearly five years of work and resulted in a DNA template without promoter or transcriptional control sequences. The first peptide- and protein-producing synthetic genes were created in 1977 and 1979, respectively [2, 3]. Steady advancement has led to recent synthesis of complete gene clusters tens of thousands of nucleotides in length, and ultimately a bacterial genome approximately 1.1 million bases in length [4].
Molecular Cloning
It is impractical for most synthetic biologists to synthesize more than several kilobases of completely synthetic DNA. It is often desirable to build bigger pieces at lower costs and faster speeds than de novo synthesis is currently able to accomplish.
Over the years, many different strategies have been developed to assemble DNA in flexible ways that suit different purposes. These strategies typically employ purification of enzymes that are known to modify DNA in specific ways and these methods of action can be exploited for designing and building specific sequences of DNA. For example, restriction enzymes are used to cut DNA in a specific manner upon recognition of a specific nucleotide sequence. Polymerases and endonucleases add or remove nucleotides to make double stranded DNA from a single stranded template or to create single stranded DNA from double stranded DNA. Many of the modern techniques take advantage of recombination machinery that break DNA from one location to reattach it to another location. In other cases, the endogenous enzymes in a host are utilized to manipulate DNA without the need for prior purification. For example, in some methods endogenous DNA ligase is used to repair single stranded breaks (known as "nicks") to complete the formation of fully circular DNA.
Ultimately, these methods generally require transformation into a host where endogenous enzymes are used to complete the genetic manipulation and replicate (clone) the genetic material. This allows for the expression of the desired proteins to test the ability of the engineered system or for the purification of the genetic material itself such that it can be used for further manipulation, study, or storage.
While there are many specific protocols for the numerous methods of cloning, most share reasonable overlap in their underlying mechanisms of action. Broadly speaking, methods may rely primarily on restriction enzymes, polymerase chain reaction (PCR), or on homologous recombination [5, 6].
Restriction Enzyme
Restriction enzymes recognize a specific nucleotide sequence and then cut the DNA in such a way that results in a double stranded break. If the enzyme cuts within or near the recognition site, it is classified as a Type II restriction enzyme. Restriction enzymes can also be classified by if the DNA break results in a straight cut resulting in a blunt end or with a jagged cut resulting in a sticky end.
For example:
EcoRI digestion produces "sticky" ends,
whereas AluI restriction enzyme cleavage produces "blunt" ends:
The Registry of Biological Parts has developed standards for the type and position of restriction enzyme sites to be used when building DNA using restriction enzymes in order to ensure compatibility in the connecting of biological parts. Two of the more popular standards are named and listed below:
- BioBricks
- BglBricks
One modification to the standard restriction enzyme method is the use of an enzyme that recognizes a non-palindromic sequence. This results in the ability to cut with a single restriction enzyme and still maintain directionality of the biological part.
- CpoI directional cloning
Other methods have been developed that utilize Type IIs restriction enzymes. These enzymes cut away from their recognition site. This strategy has the advantage over Type II enzymes in their reduction of a "scar" sequence and ability to generate combinatorial libraries.
Polymerase Chain Reaction
Polymerase Chain Reaction (PCR) is a fast and reliable way of making multiple copies of specific sequences of DNA. The PCR methods typically allows for relatively simple and fast construction of genes by linking multiple shorter oligonucleotides together in a series of steps where the earlier PCR product acts as a primer that results in a longer DNA fragment that itself can act as a primer for subsequent rounds of PCR. An exception is TOPO TA cloning, which utilizes the propert of Taq polymerase to leave a single A nucleotide overhang after the PCR reaction. In combination with an optimized vector with a T nucleotide overhang and a topoisomerase that recognizes the vector at the T overhang position, the insert and vector that are annealed at the T and A nucleotides are sealed into a circular vector efficiently.
Oligonucleotide precursors are linked together leaving gaps that must be filled in by DNA polymerase. In the most common method, polymerase cycling assembly (PCA), DNA oligonucleotides are designed to be part of either the top or bottom of the final DNA duplex. Cycles of annealing and polymerase extension result in a growing DNA duplex built from smaller oligonucleotide fragments. A final round of PCR is done with constant primers that amplify the complete desired gene product. Initial oligo linkage is done for 20-30 cycles and full-length PCR is carried out for 20-30 additional cycles.
Oligos must be designed carefully to have similar melting temperatures and be free of interfering secondary structures (high GC content, hairpins, etc.)
- Ligation Independent Cloning (LIC) [11][12][13]
- TOPO TA Cloning (Invitrogen) [14][15]
- Uracil-Specific Excision Reagent (USER) (NEB) [16]
- Splice by Overlap Extension (SOE) PCR [17][18]
- Polymerase Cycling Assembly (PCA) [19]
- Cre/Lox P1 phage (BD Creator from Clontech)
- att lambda (Gateway Cloning from Life Technologies) [25][26]
Gibson Assembly
In 2009, Daniel Gibson of J. Craig Venter's group reported a method for assembling hundreds of kilobases of DNA sequence using an isothermal enzymatic setup known today as "Gibson Assembly" [27][28][29][30]. Sub-fragments of the eventual assembly product are obtained as blunt-ended double-stranded DNA molecules several kilobases in length. They share several hundred bases of homology at their adjacent termini. T5 DNA Exonuclease chews back the double-stranded DNA molecule to reveal 3' single-stranded overhangs. Being heat-liable, T5 exonuclease is inactivated after several minutes at the reaction temperature. A mixture of thermostable ligase (Taq) and DNA polymerase (commonly Phusion) repair and assemble the individual fragments into larger molecules. This method has been used to assemble nonfunctional DNA molecules up to 900 kilobases in length, as well as the complete 16.3 kilobase mouse mitochondrial genome [31]. The size limit of molecules that can be assembled in this manner is currently limited to the cost of design and screening the final assembly products and the hundreds of kilobases of DNA that must be sequenced. An illustrative video of this process can be found on the NEB website.
- CPEC circular polymerase extension cloning [32]
- SLiCE (Seamless Ligation Cloning Extract) in vitro homologous recombination [33]
In Vivo
- Mating-assisted genetically integrated circuit (MAGIC)-bacterial mating [34]
- ET recombination [35]
- Transformation-associated recombination [36]
Moving Forward: Scaling Up Gene Synthesis
The methods below are not new methods and some are already being used commercially. What they offer for the future are methods for potentially scaling up production while decreasing costs. As mentioned before, cost and speed are major limitations preventing de novo DNA synthesis from becoming standard practice.
Solid-Phase Synthesis
An alternative gene synthesis technique using solid-phase synthesis of a DNA duplex has been created by several companies, including Blue Heron. In these methods, DNA oligonucleotides are synthesized as in other techniques, they are annealed into complementary short duplexes without overhanging ends, and these duplexes are chemically ligated on a solid phase such as a liquid chromatography column. The complete synthetic DNA duplex is then eluted and used for downstream processes, such as cloning or gene expression.
Multiplex/Microchip Synthesis
DNA oligonucleotides have become a commodity and the price per base has been steadily dropping. But there is still great expense involved in large-scale gene synthesis. For a several kilobase gene fragment, oligo cost alone can still exceed $1,000. To over come this hurdle, multiplex parallel gene synthesis technologies are under development, including inkjet DNA printers, photolithography and electrochemical parallel synthesis.
Microfluidic microchips are a common multiplexing solution. In the method of Tian et al. [37], oligonucleotides are synthesized while coupled to a microchip at their 3' end. The overall process is similar to traditional oligo synthesis, except that individual wells are photoactivated before addition of the next base onto the 5' end. After chemical cleavage of the complete oligo from the microchip, they are hybridized and purified using complementary "quality assurance" oligos bound to magnetic beads. This method eliminates oligos that have either incorporated extra bases or undergone deletions. Final yield from each synthesis well is only around 5 fmol of oligo, which is not sufficient for downstream applications. A final amplification step using common PCR primers to all oligos increases yields up to a million-fold. Oligos can then be assembled by overlap PCR-based methods such as polymerase assembly multiplexing, or PAM. This method has resulted in the synthesis, amplification and expression of a 14.5 kb, 21-gene 30S ribosomal protein operon from E. coli.
Minimizing Errors
One major roadblock remaining in synthetic gene synthesis is overcoming natural error rates in DNA synthesis chemistry. Phosphoramidite oligo synthesis currently has an error rate of 1 in ~160. This results in a high enough error rate in assembled genes that screening and sequencing are major cost bottlenecks, as is the repair or re-synthesis of mutated genes. While the development of cheap and high-throughput sequencing technologies will streamline the screening process, oligo synthesis error rates must also be reduced.
Currently, oligo pools are often screened against complementary reference libraries that can be reused. Oligos that do not completely base-pair with their designed sequence are either washed away in purification steps or are cleaved by DNA mismatch repair proteins due to their imperfect base-pairing. This has currently decreased error rates to 1 in 106, on the order of error rates in DNA polymerases.
Genome Synthesis
J. Craig Venter's team at JCVI have worked to carry gene synthesis to its most advanced level - the creation of a completely synthetic genome. Initially, the JCVI team synthesized a 582,970-base pair "minimized" genome of Mycoplasma genitalium using a stepwise assembly [27]. The genome was divided into 24 cassettes of about 24 kb each which were synthesized by commercial manufacturers. These overlapping cassettes were enzymatically stitched together into bacterial artificial chromosomes, which were attached in a stepwise fashion using a combination of restriction digestion and in vitro recombination. At the point where the molecules were "quarter genomes", they were transformed into yeast where in vivo recombination machinery assembled the full M. genitalium chromosome. This was only proof of synthesis, however, and not of a synthetic genome useful for supporting a living organism.
In 2010, the JCVI team reported the creation of a bacterium controlled by a fully synthetic genome[4]. The 1.08 mega-base pair genome of Mycoplasma mycoides was synthesized and transplanted into a M. capricolum recipient cell to create synthetic bacterium nicknamed "Synthia". The genome was divided into 1,078 1-kb cassettes, which were synthesized by Blue Heron. These cassettes were assembled in yeast using in vivo homologous recombination using a three-step hierarchy that built fragments of ~10,000 bp, ~100,000 bp and finally the complete 1.08 mega-bp genome. These were then isolated carefully from contaminating yeast DNA and transplanted into the recipient bacteria. A selectable marker placed in the synthetic genome demonstrated that bacteria that grew after transplantation were controlled by the synthetic genome. The same team demonstrated that a semi-synthetic genome could be assembled and transplanted using a combination of natural and synthetic DNA.
In 2011, a group from Johns Hopkins reported the first partially synthetic eukaryotic genome. They edited approximately 1% of the Saccharaomyces cerevisiae genome to eliminate introns, repetitive sequence and retrotransposons, replaced all TAG stop codons with TAA, and introduced recombigenic LoxPSym sites to aid in future large-scale manipulations of the genome [38].
Overall error rates in synthetic genome assembly were controlled by careful cassette synthesis and verification, as well as relying on high-fidelity in vivo recombination methods for hierarchical construction. Even low error rates can have huge effects, though. One single base deletion in the genome was in an essential gene, dnaA, and delayed progress for many weeks. Isolation, growth and manipulation of mega-base pair DNA molecules either in vivo or in vitro is a difficult roadblock as well.
Final assembly of large double-stranded DNA products into kilobase and megabase functional molecules, free of errors and with minimal cost remains a process bottleneck for gene and genome synthesis. In vitro recombination methods have been successfully used to assemble large DNA molecules on the order of tens of kilobases, but this method remains prone to errors and requires considerable homology be engineered into assembly fragments.
See Also
- Gibthon - Gibson assembly design program
- j5: A tool for designing DNA assembly with recombination from Nathan Hillson The manual has an excellent overview of recombination-based cloning strategies like SLIC and Gibson. [39]
- Review of Cloning techniques from ASU
Error fetching PMID 412251:
Error fetching PMID 85300:
Error fetching PMID 15616567:
Error fetching PMID 18218864:
Error fetching PMID 19363495:
Error fetching PMID 19745056:
Error fetching PMID 20488990:
Error fetching PMID 20935651:
Error fetching PMID 21601685:
Error fetching PMID 21918511:
Error fetching PMID 21601682:
Error fetching PMID 21364738:
Error fetching PMID 22126803:
Error fetching PMID 21750718:
Error fetching PMID 18985154:
Error fetching PMID 19649325:
Error fetching PMID 17293868:
Error fetching PMID 22328425:
Error fetching PMID 20385581:
Error fetching PMID 17907578:
Error fetching PMID 16289702:
Error fetching PMID 17389646:
Error fetching PMID 2357375:
Error fetching PMID 19111926:
Error fetching PMID 7590320:
Error fetching PMID 2235490:
Error fetching PMID 7580902:
Error fetching PMID 9321675:
Error fetching PMID 9771703:
Error fetching PMID 11076863:
Error fetching PMID 17702758:
Error fetching PMID 8552668:
Error fetching PMID 15731760:
Error fetching PMID 22241772:
Error fetching PMID 3045756:
Error fetching PMID 2020554:
Error fetching PMID 1658796:
- Error fetching PMID 4571075:
- Error fetching PMID 412251:
- Error fetching PMID 85300:
- Error fetching PMID 20488990:
genome replacement - Error fetching PMID 21601682:
Gene Synthesis Review - Error fetching PMID 19111926:
review - Error fetching PMID 18985154:
GoldenGate - Error fetching PMID 21364738:
MoClo - Error fetching PMID 22126803:
MoClo - Error fetching PMID 21750718:
GoldenBraid - Error fetching PMID 2235490:
LIC - Error fetching PMID 7580902:
LIC - Error fetching PMID 9321675:
LIC - Error fetching PMID 2020554:
TA cloning - Error fetching PMID 1658796:
TOPO - Error fetching PMID 17389646:
USER - Error fetching PMID 3045756:
SOE-PCR - Error fetching PMID 2357375:
SOEing - Error fetching PMID 7590320:
PCA - Error fetching PMID 17293868:
SLIC - Error fetching PMID 22328425:
SLIC - Error fetching PMID 17907578:
in-fusion - Error fetching PMID 16289702:
in-fusion - Error fetching PMID 20385581:
In-Fusion biobrick - Error fetching PMID 11076863:
Gateway lambda Int - Error fetching PMID 17702758:
Gateway lambda Cre - Error fetching PMID 18218864:
Complete genome synthesis - Error fetching PMID 19363495:
oligonucleotide assembly in vitro - Error fetching PMID 19745056:
oligonucleotide assembly in yeast - Error fetching PMID 21601685:
MIE paper - Error fetching PMID 20935651:
- Error fetching PMID 19649325:
CPEC //T5 exonuclease recombination
- Error fetching PMID 22241772:
SLiCe - Error fetching PMID 15731760:
MAGIC, bacterial mating approach - Error fetching PMID 9771703:
ET recombination - Error fetching PMID 8552668:
Transformation-associated recombination (TAR) cloning - Error fetching PMID 15616567:
- Error fetching PMID 21918511:
- j5 DNA Assembly Design Automation Software doi: 10.1021/sb2000116


text:
RoadsideThoughs depends on cookies for its operation. Please refer to our Privacy page for details. By closing this banner or continuing to browse RoadsideThoughts, you agree to the use of cookies.
Below are places that are close (or relative) to Killarney.
This page contains five sections:
The largest community within a 50 mile [80 km] radius is Edmonton. It lies immediately south of Killarney. Edmonton has a population of 812,000 people.<1>
The next largest community is Saint Albert. It lies just northwest of Killarney and has a population of 57,700 people.
The following large communities are also in the area. Keep in mind that when we give a distance, it's a 'straight-line' distance starting from Killarney and not the distance if you are driving it.<2>
The following is a sample of neighboring communities:
As we travel, we always find names that give pause: while some are interesting and others familiar, some names are simply curious along with those that just strike our fancy. We always talk about such a name and if time permits, we'll try to make a side-trip.
We have a new section that have been added to our Genealogy Helper Page for Killarney.
We have a list of several cemeteries that are in the area around Killarney. Our list includes the location of each cemetery, with the ability to see an overhead view of the cemetery.
Of the airports that we know about, these are some of the closest to Killarney.
When we're on the road, rather than always picking an airport that is close to our destination, we try to see if there might be another airport that would be a better fit to our travel plans.<5> To help with your planning, the following list of airports is organized by type and where they are relatively close to Killarney.
Please keep in mind that these airports where chosen based on their air-distance from Killarney. You should check the map and see how accessible they are when considering one airport over another.
Large Airports
Smaller Airports with Scheduled Flights / Air Taxi Service
General Aviation Airports
Additional Airport Information
For each of the airports listed below, we've linked to websites where detailed information about the airport can be found.
Cities, Towns and Villages of Alberta
About Alberta
Unfortunately, we don't know of a website for Killarney. If you can help, please contact us through our Feedback Page.
The official website for Province of Alberta: www.alberta.ca/
- Return to Citation
For additional information, please visit our Glossary for Killarney.
Please keep in mind that we can not guarantee the accuracy or timeliness of the information on this website, so use with care. We encourage you to double-check the information that is critical to you.
If you've found an error or have additional information that you would like to share, please don't hesitate to write. Click here to contact us.

subset: commoncrawl


text:
When a bold statement is the goal, the Somerset House carpet collection offers high-impact, fashion-forward choices to help you define your design.
The beauty of natural fiber is the foundation for this stylish, stand-out collection of woven wiltons, wool berbers, flat weaves, sisal, coir and printed wools.
View the entire Somerset House CollectionColor | Larkspur |
Color Family | Light Neutrals |
Style | Loop |
Fiber | Triexta |
Fiber Brand | Smartstrand Ultra Forever Clean |
Construction | Continuous Filament |
Size | 12' |
Pile Height | 0.33 |
Face Weight | 38 |
Stiches Per Inch | 8.33 |
Pattern Match | 0.156 |
Backing Material | H4 |
FHA Certified | Yes |
Origin | US |
Application | Residential |
ProSource offers products from industry-leading suppliers, with warranties that allow you to feel confident with your product selection. See below for more information.
Bring a written quote into your ProSource Wholesale Showroom at the time of purchase. If, for any reason, ProSource can't beat a competitor's first-quality, non-closeout price on identical merchandise, we'll give you $100 on the spot. Guarantee applies to exact brand comparisons only. Each showroom is independently owned and operated. Products and prices may vary by location.


text:
Send News. Want a reply? Read this. More in the FAQ. News Forum - All Forums - Mobile - PDA - RSS Headlines Twitter |
The Crysis 3 Website now offers system specifications for Crysis 3, the next installment in Crytek's shooter series (thanks VG247). They outline the typical minimum and recommended systems for playing the game, as well as a "hi-performance" spec for the ultimate experience:
Copyright © 1996-2017 Stephen Heaslip. All rights reserved.
All trademarks are properties of their respective owners.
News CGI copyright © 1999-2017 James "furn" Furness &
Blue's News.
All rights reserved.
Chatbear v1.4.0/blue++: Page generated 30 March 2017, 17:01.
Chatbear Announcements.


text:
The Genealogy & Family History Social Network
You must be a member of Genealogy Wise to view this page.
Join Genealogy Wise
Welcome toGenealogy Wise
Sign Upor Sign In
National Institute for Genealogical Studies
Genealogy Store
Get Badge
© 2017 Created by Nat Ins for Genealogical Studies. Powered by
Badges | Report an Issue | Terms of Service
Please check your browser settings or contact your system administrator.


text:
Home | Help | Search | Link Exchange | ToS/DMCA | Contact | Login | Register |
Pages: [1] Go Down |
Author | Topic: Members can use Twitter #hashtags and @ tags to boost visiblity of new topics! (Read 3316 times) |
Content such as images and videos posted by members are NOT hosted on Anarchy Nation servers. They are hotlinks where the images are hosted elsewhere, and the links point to these these third party sites. None of these sites are affiliated with Anarchy Nation. Do not submit DMCA requests to the owners of this site. Instead follow the links, and request the files themselves be removed from the file hosting site's servers. Learn more about our DMCA Policy by clicking here. Anarchy Nation takes no responsibility for what any user encounters when following a link outside this website. This includes (but not limited to) advertisements, pop-ups, embedded adware/malware, suggestion of paid subsriptions, etc. We highly encourage users to have proper firewall, anti-virus and malicious script-blocking software installed on their platforms. It is better to be safe than sorry! |


text:
Bronco, Excursion, Expedition, Explorer Ford Steering Stabilizers
Rancho high performance steering stabilizers for your Ford are a simple and affordable way to enhance a vehicle's performance. Rancho steering stabilizers are ideal for off-road driving, towing / hauling and street performance. All Rancho products are covered by a Limited Lifetime Warranty.
Vehicle | Type | Year | Price | Item # |


text:
On Sale - ProjectoScreen120HD & 144HD
Epson Refurbished Projectors
Save with Epson Refurbished Projectors
Our Price Beats: $375.00
Our Price: Click for Special Price
Overview: 87696
Front or rear projection surfaces snap easily on to the front of the silver aluminum frame. Flat back and sides for easy installation. Provides a perfectly flat viewing surface for graphic and data video projection.Da-Mat, High Contrast Da-Mat, Da-Tex (Rear), Pearlescent, Cinema Vision, High Contrast Cinema Vision and Dual Vision fabrics up to and including 16' high will be seamless. Audio Vision, High Contrast Audio Vision and High Contrast Cinema Perf fabrics up to and including 8' high will be seamless. High Power fabric up to and including 6' high will be seamless.
**Screen Ordering Notes:Please allow 48 hours to process screen orders.Screens are only shipped in Contiguous US (48 states).Screen dimensions are based on manufacturers details and subject to change. Tolerances are typically ±1 inch.


text:
Shop By
Currently Shopping by
- Frame Size: Remove This Item 14ft x 16ft
Trampoline Nets
Australia customers, Click here
Measure your outer frame size to buy the right size net.
We offer Trampoline Nets for all major brands. Scroll down on the left to narrow your search.Measure your metal frame size. From one side to the other, across the middle.
Count the number of poles. Match your trampoline to the pictures.
Netting only for Octagon, Oval, Rectangle, Round, Square Trampolines.
Narrow your search, scroll down on the left.
We also sell complete enclosure systems with Netting and Poles. Click on Trampoline Enclosures - Complete on the left.
Regular Price: $142.00
Special Price $98.00
Trampoline Replacement Net for 16ft x 14ft Oval - 6 pole Enclosure - NET ONLY
NET ONLY, poles and hardware not included. Fits 16x14' oval frame with 6 angled poles and straps. -
Regular Price: $142.00
Special Price $98.00
Net for 14ft x 16ft Oval Trampoline Enclosure using 6 poles
Net only, poles not included. Original Skywalker Trampolines Brand.


text:
Gene Baker : Celebrity Addresses
Claim to Fame:
Address 1: Gene Baker
2250 E 48th St Davenport, IA52807
Latest Mail Result:
No results yet. Please submit one!
Submit Your Mail Results | Mail Results History (Your results help all community members!)
⇒ Report this address as outdated.
⇒ See "Member Tools for this celebrity" near the bottom of this page.
Member Tools for this Celebrity
⇒ Submit an address for Gene Baker ⇒ Request an updated address for Gene Baker ⇒ Setup email alerts for Gene Baker. ⇒ Members Helping Members - Please help other members! ⇒ Report Gene Baker is deceased Other Tools
Our celebrity address database and community has thousands of addresses, members, and mail results! Celebrities A-Z


text:
There are no extensions by this developer currently available on Magento Connect.
Currently there are no reviews for this developer.
Please include your feedback on this Developer in your extension review.
We built Magento Connect using the latest techniques and technologies.
This makes Magento Connect faster and easier to use.
Unfortunately, your browser doesn't support those technologies.
Use the links below to download a new browser or upgrade your existing browser.
Internet Explorer Version 9+ Mozilla Firefox Version 7+ Google Chrome Version 14+ Apple Safari Version 5+


text:
CONCORD - On Friday, Aug. 22, the Old Manse, where several New England transcendentalist authors lived and gathered for inspiration, will open its doors free of charge to all visitors.
The Concord literary landmark will be participating for the first time in the Free Fun Fridays program, organized and funded by the Highland Street Foundation.
A National Historic Landmark where Ralph Waldo Emerson and Nathaniel Hawthorne once lived, it was built in 1769 by Emerson�s grandfather just yards from the spot where the first shots of the American Revolution would later be fired.
From 10 a.m. to 8 p.m., the Old Manse will offer the following activities at no charge: admission to the historic home, including self-guided and kid-friendly tours; Colonial music and dance demonstrations; a scavenger hunt and craft demonstrations; living history and a recreation of an 18th century yard; guided attic and landscape tours; tours of the adjacent Caesar Robbins House offered in partnership with the Drinking Gourd Project and more. Food and drink will be available for purchase.
Managed by the Trustees of Reservations, the Old Manse is located at 269 Monument St., Concord.
For more information, call 978-369-3909 or visit www.thetrustees.org/old manse.
Admission is free to the Old Manse on Friday
Aug 21, 2014 at 2:11 PM
Aug 21, 2014 at 2:31 PM


text:
Everyone Wins at Piolets d'Or
"2012 was an exceptional year for groundbreaking ascents which reflected the values of the Piolets d'Or," the Piolets d'Or committee said in a press release. "The jury struggled to reduce that list to six, but those that were eventually chosen are truly outstanding."
"In light of the very high level of the six ascents, the 2013 Jury chaired by Stephen Venables has decided to award each of the six nominated ascents a 'Piolets d'Or.'"
The committee stated that each of the six nominated ascents was notable for reaching the summit and then coming down the mountain by a different descent route.
It was only in 2009 that the Piolet d'Or became the plural Piolets d'Or, allowing for multiple awards each year.
The following six ascents were nominated for and won the 2013 award:
Baintha Brakk, a.k.a. The Ogre (7,285m), Pakistan
Josh Wharton, Kyle Dempster and Hayden Kennedy made an expedition to complete a first ascent via the south face of The Ogre. Dempster and Kennedy reached the top but had to descend with an ill Wharton.
Nanga Parbat (8,125m), Western Himalaya, Pakistan
Rick Allen and Sandy Allan of the U.K. made the first successful traverse of the Mazeno Ridge in Pakistan. At six to eight miles long, this is one of the longest ridges in the Himalaya. They were also accompanied for most of the traverse by Cathy O'Dowd of South Africa.
Kyashar (6,770m), Nepal
Hiroyoshi Manome, Tatsuya Aoki and Yasuhiro Hanatani of Japan climbed the 2,200m south pillar of Kyashar. At least seven other times had tried and failed on this same route.
Muztagh Tower (7,284m), Pakistan
Alexander Lange, Dmitry Golovchenko and Sergey Nilov of Russia took 17 days to climb the 2,000m northeast spur of this unclimbed Karakoram peak.
Kamet (7,756m), India
Didier Jourdain, Sebastien Bohin, Sebastien Moatti and Sebastien Ratel of France summited Kamet via the previously unclimbed southwest face and then descended via the south face.
Shiva (6,142m), India
Paul Ramsden and Nick Fowler of the U.K. traversed the Prow of Shiva by climbing the northeast ridge and descending the south ridge in the fifth overall ascent of the mountain.
You can read more about the six Piolets d'Or winners on the Rock and Ice website here. To read about the Special Mention by the committee to two "fair means" ascents of Cerro Torre, click here.


text:
Rosie Woven Oversized Shirt
Style Notes
Day tops are a staple separate in every wardrobe
Make your top pop this season with sporty, baseball-style basic tees in quilted finishes with ribbed, stripe trims. Crew necks come in block colours, crop tops with mesh inserts and long sleeve jersey staples with the hot-right-now high neck. Kick-start your casuals by layering them under out-there outerwear – bomber jackets are best for teaming with a t-shirt, while duster coats take turtle necks up a notch for the new season.
Details & Care
100% Polyester. Flat Measurement of Garment Not Worn. Total Length 47cm/18". Measured On UK Size 10. Machine Washable. Model wears size 10. -
Shipping info
Delivery option Delivery times Delivery cost Super Saver Delivery 9 working days $5.99 per order Standard Delivery Up to 6 working days (Metro areas only). $6.99 per order Express Delivery Up to 4 working days (Express delivery may take longer in very remote areas) $15.00 per order Click here for further information.
Returns info
We hope you are satisfied with all of your purchases but if you ever need to return an item, you can do so within 28 days from the date your parcel was dispatched.
Please note, we cannot offer refunds on pierced jewellery or on swimwear if the hygiene seal is not in place or has been broken.
Click here to view our full Returns Policy.


text:
19456 Surnames | 123976 Records | 2652 Reunions
Dead Fred asks:Are you a Desperate Genealogist? Click the e-book above for details!
Dead Fred's Genealogy Photo Archive, a free, fun photo genealogy research web site devoted to helping you visualize your heritage!
Photo Thumbnail Click image for larger view.
Order The Desperate Genealogist's Idea Book: Creative Ways to Outsmart Your Elusive Ancestors (eBook).You can also order The Desperate Genealogist's Idea Book in hardcopy format by clicking here.
Photo #31169
Last Name: Williams
First Name : LaMar
Middle Name:
Subject's City: Nephi
Subject's State: UT
Subject's County: Juab
Subject's Country: United States
Date: 1921-1940
Photographer: Chapman
Photographer's City: Nephi
Photographer's State: UT
Type** : B&W
Comments: LaMar Williams (Nee: ) | Nephi UT United States | 1921-1940 | Comments: Photo of the Juab High School Class of 1937 on the front steps of Juab High School, Nephi, Utah (from the scrapbook of Wanda Ingram Bowers). Row 1 Lillian Peterson Zelma Bowers Theo Stephenson Rayola Shepherd Barbara Petty Minnie Howard Geraldine Bowles Myrtle Bailey Clarice Kendall Genevieve Sudweeks Maxine Park Alice Christiansen Marjorie Jackson CoDeal Mangelson Nancy Radley Row 2 Phyllis Francom Grace Garrett Sarah Aagard Henrietta Ballam Jane Lunt Wanda Ingram Geraldine Cazier Delma Ostler Afton Hansen Kathleen Wells Grace Blackett Ora Sherwood Melva Douglas Grace Powell Maurice Chapman Bryan Forrest Row 3 John Gadd Arthur Sidwell Merrill Stephenson Frank Gustin ----- Winters Warren O'Gara Jack Pace Wilson Foote Row 4 Tom Hall LaMar Kendall Harry Duckworth Blaine Greenhalgh Morgan Greenwood Howard Painter Max Bowles Rulon Anderson Joseph Pastrup Clair Kendall Keith Chase Glen Pratt Phil Garrett Don Bowles Clifford Garbett LaMar Williams Roscoe Garrett Row 5 Jimmy Nielsen Melvin Wynder Don Gowers Grover Brough Ross Golden Frank Foote Keith Kendall Clark Sparks Eldon Boswell
Digital Image Only? NO
Everyone Listed In This Photo:
To view all records posted by this submitter, click here:
Home Search Photos | Post Photos | Surnames | Mysteries | Annuals Meet Fred | Testimonials | Media | Archivists | FAQs Contact Us | Groups | Links | Your Own Search Box | Marketplace Privacy Policy | Terms of Service
Free genealogy searches for those looking into their family history.
© 2001-2017 DeadFred.com Genealogy Photo Archive P.O. Box 6937 | Springdale, AR | 72766-6937 | (479) 750-0082
Search Photos
Post Photos
Meet Fred
Media Center
Contact Us
Facebook Group
Your Own Search Box


text:
Type |
Website Templates |
Author |
Middltone |
Package |
Responsive Template |
Additional Features |
Back To Top Button, Crossbrowser Compatibility, Dropdown Menu, Favicon, Google map, Google Web Fonts, Sliced PSD |
Animation |
HTML plus JS |
Coding |
CSS 3, HTML 5, JQuery, Sass, Semantic Code, Valid Coding |
Features |
On-line chat, Responsive |
Gallery Script |
Slider, TouchTouch |
Kind of shops |
Use this responsive Website template for Environmental, Business, Industrial, and Science websites. |
Language support |
English |
Topic |
Business & Services, Environmental Templates |
Web Forms |
Contact Form |
Width |
2050px |
Sources Available
HTML PSDSoftware Required
Adobe Photoshop CS+
For uncompressing a template ZIP package: WinZip 9+ (Windows); Stuffit Expander 10+ (Mac)
Sublime Text2 or later, Notepad++
Every website template here is a perfect example of what a perfectly executed HTML5 design can look like. Fully responsive and cross-browser compatible these professional web templates come in a wide selection of thematic categories. We are sure you'll find the one template your online business needs to become a true success.


text:
Proteins with unstable 3-D structures help the microscopic animals withstand drying.
Researchers find strong correlations between the composition of the human microbiome and genetic variation in immune-related pathways.
Researchers find strong correlations between the composition of the human microbiome and genetic variation in immune-related pathways.
The discovery of a new form of the deadly botulinum toxin gets published, but its sequence is kept under wraps until an antidote is developed.
Nobels awarded for vesicle trafficking and computational chemistry; building 3-D microbial communities; mislabeled microbes cause retractions
Researchers apply a 3-D printing technique to structure populations of bacteria in a three-dimensional environment.
A new tick-borne disease has emerged in the US Midwest—and the culprit is not a bacterium.
Antibiotic cycling makes a comeback in the lab; how life scientists can learn from astronauts; napping to conquer fears; deconstructing the cancer R&D crisis
Switching up the drugs used to treat bacterial infections could help clinicians battle both illness and resistance at the same time.
A patient response-based gene expression signature can distinguish respiratory infections caused by viruses from those of bacterial or fungal origin.
The agency estimates that at least 23,000 people in the U.S. die each year as a result of antibiotic-resistant infections.
Researchers behind high-impact studies that at first seemed obscure are honored in another round of prizes.


text:
The European Patent Office will grant patent rights over the use of CRISPR in all cell types to a University of California team, contrasting with a recent decision in the U.S.
Deriving physiologic first principles
The history of cetaceans can serve as a model for both evolutionary dynamics and interdisciplinary collaboration.
On islands off the coast of Florida, scientists uncover swift adaptive changes among Carolina anole populations, whose habitats were disturbed by the introduction of another lizard species.
Fossils of an extinct, armored fish challenge current understanding of when copulation and internal fertilization evolved in jawed vertebrates.
Genes acquired from bacteria contributed to the origins of archaeal lineages, a large-scale phylogenetic analysis suggests.
Simple changes help yeast thrive in the presence of their own harmful byproducts and could boost biofuel production.
Researchers have captured footage of wild chimpanzees teaching each other to use tools, lending support to the idea that humans aren't the only primates to engage in social learning.
In chapter 5, "Resurgence: Bedbugs Bite Back," author Emily Monosson chronicles the rise of the pesky pests in the face of humanity's best chemical efforts.
Extinct, giant arthropods, long assumed to be top predators of ancient seas, didn't have sharp enough eyesight to be refined hunters.
Bed bugs are but one example of a species whose populations have evolved in response to human behavior.


text:
Jazz2Online © 1999-INFINITY (Site Credits). Jazz Jackrabbit, Jazz Jackrabbit 2, Jazz Jackrabbit Advance and all related trademarks and media are ™ and © Epic Games. Lori Jackrabbit is © Dean Dodrill. J2O development powered by Loops of Fury and Chemical Beats.
Original site design by Ovi Demetrian at GotToSeeIt.com. DrJones is the puppet master. Eat your lima beans, Johnny.


text:
By registering i agree to the Terms & Conditions.
Already a member ? Please Log In.
Not a member yet? Go to the quick Sign Up !
Enter your information in the form below and we will get back to you within 24 hours.
--- Number of Employees --- 201 or more Employees 51 to 200 Employees 26 to 50 Employees 11 to 25 Employees 6 to 10 Employees 1 to 5 Employees
--- Industry --- Accounting / CPA Advertising Agriculture Architecture Auto Dealership Banking & Financial Services Construction / Contracting Consulting Distribution Education Energy & Utilities Engineering Food / Beverage Government Healthcare / Social Services Hospitality / Gaming Human Resources Insurance Legal Maintenance / Field Service Manufacturing Marketing Services Media & Communications Non-Profit Pharmaceuticals Property Management Public Sector Real Estate Retail Software / Technology Sales & Marketing Telecommunications Travel, Transportation & Logistics Other
We haven't collected enough critic reviews for this product yet.The cloudswave score requires at least 3 critic reviews to be computed.
Key feature
Subscribe for free and get updates on new web apps and exclusive deals.
Thank you for subscribing to our newsletter !
Enter your email and press continue to start downloading.
The white paper has been sent to your email. You can also download it right now from this URL: Detailed comparison of the 10 best Academic Resources software


text:
I picked up this skirt at a Goodwill a few weeks ago. I was completely attracted to its long length and sheer fabric. Im also really in love with (cause I cant voice it enough) my Rackk and Ruin crystal/bullet necklace. Im going crazy over the insanely beautiful amethyst, especially since my palm reading Ive been more into crystals and their healing powers. Amethysts are said to:
- ward of negative influences
- calm stress
- enhance spiritual awareness
P.s. For those of you wondering, my sun is Taurus, my moon is Scorpio and Im a Cancer Rising.


text:
Advertisement:Close [X]
Lingerie Sex VideosDo you share our lust for extreme lingerie? See our creamy thighs wrapped in stockings, our sheer pantyhose toes jerking throbbing cocks. From latex to lace we get hot and our pussies drip when we slide into something kinky and spread wide!
Blonde masturbates in thigh highs and high heels
Want to subscribe to this channel?Sign in to add this channel to your list
Sign in or RegisterPlease confirm your email address
to use this functionality


text:
Jordynn Warmer
by Heidi May
Welcome! You are browsing as a guest
Would you like to see
15 projects made from this pattern
and much more?
create a free account
What am I missing?
© Heidi May
© Heidi May
© Heidi May
Jordynn Warmer
by Heidi May
This pattern is available
for $5.50 USD
buy it now
Welcome to The Velvet Acorn, here you will find purely original pattern designs in knit and crochet. Inspired and crafted with my love of nature and the outdoors in mind. I always aim for comfort, warmth and versatility, timeless pieces that layer well and go with everything. I always create trying to pay close attention to detail, making every pattern design unique and filled with love and personality all their own.
Thank you for looking and keep an eye out for more patterns to come.
All pictures and patterns copyright to The Velvet Acorn ©
About this pattern
by Heidi May
15 projects,
in 55 queues
About this yarn
by Cascade Yarns
90% Wool, 10% Other
128 yards
842 votes
3422 projects
stashed 1955 times
More from Heidi May
- Page created: January 10, 2012
- Last updated: September 4, 2016 …
- visits in the last 24 hours
- visitors right now


text:
- EXCLUSIVE OFFER: Jim Cramer's Protégé, Dave Peltier, only buys Stocks Under $10 that he thinks could potentially double. See what he's trading today with a 14-day FREE pass.
Editor's Note: TheStreet ratings do not represent the views of TheStreet's staff or its contributors. Ratings are established by computer based on metrics for performance (which includes growth, stock performance, efficiency and valuation) and risk (volatility and solvency). Companies with poor cash flow or high debt levels tend to earn lower ratings in our model. NEW YORK ( TheStreet) -- Safeway (NYSE: SWY) is trading at unusually high volume Thursday with 11 million shares changing hands. It is currently at two times its average daily volume and trading up $1.01 (+5.8%) at $18.47 as of 10:55 a.m. ET.


text:
- Frequently Asked
- How You Can Help
Prefix Information
Find the geographical location of any
telephone number / prefix in
any area code.
Search in Area Code 639
This is a directory of U.S./Canada/Caribbean telephone prefixes and their
geographical locations. Each area code has its own search page.
This telephone number /prefix location search service
is compiled from available information. If you know of a prefix we do not
have or you spot an error, let us know but don't get upset about it.
We do the best we can.
This prefix list is updated on a regular basis.
We know about ALL new area codes long before they are implemented. If you
don't see the new code yet, go back to the old code for the prefix location.
Caveat: Although we have done our best to provide accurate information,
errors can and will creep in. We will correct any and all errors that we find
or are brought to our attention at the next update.
You can only look up ONE area code/prefix at a time and only by typing in
a three number prefix. Typing in anything else will just give you an
"error message" as the program will not be able to find anything but ONE
prefix in that specific area code.


text:
Pawn a cloth gazebo for Cash!
Get cash offers from pawn shops near you.
Find a Pawn Shop Near Me
You might also like...
We've seen people pawn everything from jewelry, gold, silver, diamonds, firearms, musical instruments, boats, and sports cars to even a jet pack! Our blog covers it all.


text:
Some tires may be discontinued and not available.
We'll notify when this product is available
Touring A/S 205/60R16
Features & Benefits
Tire Specifications
Touring A/S 205/60R16
Size | UTQG | Max. Load | Inflation Pressure | Tread Depth | Tire Weight | Rim Width Range | Sect. Width | Tread Width | Overall Diam |
205/60R-16 | N/A | 1,389 lbs | N/A | N/A | 23 lbs | N/A | N/A | N/A | 26" |
Purchase includes the Regul Touring A/S tire only. The wheel rim shown in the picture is not included. Picture provided is a stock picture from the manufacturer and may or may not reflect the actual product being ordered.
All Regul Touring A/S tires are continuously developing with manufactured improvements. With that said, Simple Tire and the manufacturers have the right to change these tire specifications at any time without notice or obligation.
If you have any questions, please email us at [email protected]
Please Note: Specifications of the Regul Touring A/S tires shown above are listed to be as accurate as possible based on the information provided to us by Regul tires.
Available Sizes & Prices
No Similar Tires available
Touring A/S 205/60R16
Treadlife Warranty
Touring A/S 205/60R16
This product is not covered by a manufacturer's treadlife warranty.


text:
Become aware of the conscious and unconscious codes of meaning we send when communicating with others. Learn More>>
Learn how to use Microsoft PowerPoint 2010 to create exciting slide presentations that include SmartArt, multimedia, animation, sound, charts, clip art, and hyperlinks. Learn More>>
Improve your career prospects by learning how to develop powerful written documents that draw readers in and keep them motivated to continue to the end. Learn More>>
Lost for words? Don't be! Learn to build rapport, trust, warmth, and respect through conversation. Learn More>>
Learn how to use the five most popular social media platforms—Facebook, Twitter, LinkedIn, Pinterest, and Google+—to grow and promote your business. Learn More>>
Write great marketing copy to improve your company's image and your chances of getting hired or promoted. Learn More>>
Gain the respect and admiration of others, exert more control over your destiny, and enjoy success in your professional and personal life. Learn More>>
Learn how to use Microsoft PowerPoint 2013 to create animated presentations formatted with color, text, pictures shapes, charts, and text and object hyperlinks. Learn More>>
Learn the secrets of effective public speaking and small group communication. Learn More>>
Develop an Internet marketing plan for your business that incorporates SEO, advertising, email, social media, and more. Learn More>>
Complete the form below and click the Reset Password button. If we find your password, we'll send password reset instructions to the e-mail address we have on record for you.


text:
Movies |
Drama, Suspense/Thriller
Shotgun Stories
Son Hayes never speaks of the scars on his back. The shotgun pellets left under his skin make for a sporadic pattern of blue-black dots. The men he works with take bets on how he got them. His brothers, Boy and Kid Hayes, don't discuss it. His past, just like these scars, is never far behind him. This stands true for the memory of his father, a man that never bothered to give his children proper names. He left the three brothers, Son, Boy and Kid, when they were young. Their last impressions were of a violent drunk who never hesitated to put his own needs ahead of his family. The brothers were left to be raised by their mother, a hateful woman, who to this day blames her children for the life she's been left with and the man she could not keep. Their father, having left the memory of his children as completely as he left their home, managed to move on and put his life back together. He sobered up, became a devout Christian, married a wonderful woman, and fathered four new sons. All of who received proper names. His life became a model that most would aspire to, a man successful in business, community and family. His only true failing are the sons he turned his back on.
Cast: Barlow Jacobs, Michael Shannon, Glenda Pannell, Douglas Ligon, Natalie Canerday
Produced by: David Gordon Green
Directed by: Jeff Nichols
Written by: Jeff Nichols
Running time:
1hr 32min
Opens: Apr 18, 2008 NY
Theaters for Today |
Pick a day


text:
Movies |
Get Him to the Greek
Aaron Greenberg gets things done. The ambitious 23-year-old has exaggerated his way into a dream job just in time for a career-making assignment. His mission: Fly to London and escort a rock god to L.A.'s Greek Theatre for the first-stop on a $100-million tour. His warning: Turn your back on him at your own peril. British rocker Aldous Snow is both a brilliant musician and walking sex. Weary of yes men and piles of money, the former front man is searching for the meaning of life. He may have to coax, lie to, enable and party with Aldous, but Aaron will get him to the Greek.
Cast: Rose Byrne, Jonah Hill, Elisabeth Moss, Russell Brand, Katy Perry
Produced by: Judd Apatow, David L. Bushell
Directed by: Nicholas Stoller
Written by: Nicholas Stoller, Jason Segel
Running time:
1hr 49min
Opens: Jun 04, 2010 Nationwide
Theaters for Today |
Pick a day
on this day. |


text:
Language: English Continue
This country code will remain if no action is taken to change it.
Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.
Expand to Read More »
« Collapse Content
Buffer C 10X Buffer
Bovine Serum Albumin, Acetylated
MULTI-CORE™ 10X Buffer
This product can be added to a
Helix Freezer
This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
Already a Helix Customer?
Buffer C. 37°C.
Haemophilus haemolyticus.
10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
R6441 For Research Use Only. Not for Use in Diagnostic Procedures.
View All Reviews »
Review This Product »


text:
Users Online Now:
Who's On?
Visitors Today:
Pageviews Today:
Threads Today:
Posts Today:
Join Our:
GLP Poker Rooms
Donate To GLP
Adv. Search
Back to Forum
Back to Thread
I live in in Newtown, CT
User Name
Font color:
Dark Red
Dark Blue
Ms Sans Serif
In accordance with industry accepted best practices we ask that users limit their copy / paste of copyrighted material to the relevant portions of the article you wish to discuss and no more than 50% of the source material, provide a link back to the original article and provide your original comments / criticism in your post with the article.
[quote:Anonymous Coward 13806508:MV8yMTE5NzMwXzM1Nzc3ODY4X0NCMzQyNDVF] [quote:Anonymous Coward 1482838:MV8yMTE5NzMwXzM1Nzc3ODMzXzIxOTEwOTkz] [quote:Anonymous Coward 13806508:MV8yMTE5NzMwXzM1Nzc3NjQzXzY0NEVCMjVF] [quote:Anonymous Coward 33061433:MV8yMTE5NzMwXzM1Nzc3MzA5X0U2M0M1Q0FG] For those of you just catching up, the NBC news Chief Justice Correspondent Pete Williams went on national TV on 12/15 and said that Federal and state officials confirmed to him that there were no rifles in the school, just FOUR handguns. [/quote] And that afternoon officials told him that was a mistake and NBC corrected their report. At least you're being honest in pointing out the date of Dec. 15 whereas most people are trying to pass this off as a recent report. [/quote] Do you see what they did there? They took Pete Williams a former State Dept spokesman and fed him a false story, wound him up and had him parrot it on TV. Then when that didn't work.. [/quote] What do you mean by "that didn't work"? [/quote]
Original Message
I will post no proof that I actually do live here. I'm sure some have clicked away immediately at that confession and I do not blame them. You may or may not believe in what I am about to say and that is your choice.
I live in Newtown. I live about 1 mile from Sandy Hook Elementary on Riverside Road. I didn't go to work on Friday Dec 14th. I called in sick but I was playing hooky. I work at a restaurant in Danbury. I've spent the past 2 hours debating with myself if I should post this. I could have posted it somewhere else but this site seems to always pop up when the Sandy Hook "shooting" is concerned. I figured this was the most popular spot.
At around 8:50-9:00am I had just finished eating my breakfast. I had Captain Crunch cereal. I had used the last of my milk. I decided to go get some at Caraluzzi's because a friend of mine works there and I wanted to see what he was up to that evening. I get in my car at about 9:05am. I drive West on Riverside Road. Before I even got to the Fire House, I noticed a lot of vehicles parked there. Way, way, way more than usual. A few was parked on both sides of the road. I figured they was just doing some kind of training or a meeting. However one thing did stick out and that was the fact that a lot of the vehicles was solid silver and solid black. I drive past the Fire House and the Senior Center is right next door less than 100 yards. That parking lot was full as well with some of the same vehicles mixed with trucks and what not. Vehicles you would expect to see in a parking lot. Still I thought nothing of it. I go get my milk.
I'm in Caraluzzi's and I've been talking to my friend for a right good while and we hear 2 police cars go by. About 5 minutes later we hear a Helicopter. Dave comes from the back and said, "They must be doing another drill again. Scanner said mass casualty incident involving children". Right then I figured out why all those vehicles was at the Fire House and Senior Center. Because it's not the first time they have done that. They did a similar thing about 1 year ago around this time of year. And they are always doing car accidents that concerns Hazmat or whatever. I told them what I saw. Dave said, "Those cars sound like FBI cars. I guess they would be in on it". So I figure to go so I could catch a peek at what was going on. I get to town and couldn't go down Riverside Road. I told the officer that I live down there. He said, "No through traffic even for residents. Fire and Rescue and Police need to finish doing what they are doing". I said, "What are they doing?". He said, "I would tell you if I knew and I have no clue. The way it sounds they are running a drill but we have been blocked from main traffic. I was just told to do traffic control and make sure no one goes down there".
Then I drive west to Danbury. I don't know why but I just did. I was thinking to myself that that was what I get for playing hooky from work. I had the radio on but not loud enough to hear it. I turn it up thinking maybe I could catch what was going on. The local station mentioned nothing. At about 10:00 they break in from Sammy Hagar's "I Can't Drive 55". The guy said that he was hearing reports of an incident at Sandy Hook Elem and that surrounding schools was on lock down however, "I called my wife because she works at Newtown Middle School and she said they weren't on lock down and haven't heard anything about an incident. So it appears information has been mixed up. We need to get our stuff together before they tell me to report".
I get to Danbury and I go into Global gas station to get a Mt. Dew. They had the tv on and I froze. They was watching coverage of the "shooting". I just watched for 30 minutes frozen. Then I started thinking about all of those cars at the Fire Dept. I didn't know what to think about that. My brain just didn't work after watching the tv.
Fast forward to right this second. I've read the theories and the stories and honestly, those are the only ones that add up. I live here and I do not know what the **** went on. This town is fairly small. I know right many people. I know Eugene Rosen. He is an extremely creepy bastard. I know Scarlett Lewis. But I would like to know where in the hell she got all of that money to "spruce" herself up for interviews. But the Phelps and Parker families that seem to be shoved down our throat, I have no idea in hell who they are. I've asked family and friends and they don't know who they are. I know a Robert Parker, but that sure as hell isn't him in the interviews.
I just do not know what is going on.
Pictures (click to insert)
Potentially Offensive
Big Round Smilies
Aliens and Space
Friendship & Love
Misc Small Smilies
View All Categories
Next Page >>


text:
Loving these simple two tone balloons.
Your email is never published nor shared. Required fields are marked *. Please, be nice!
Notify me of further comments on this post
Want your image to appear next to your comment? Get a gravatar!
Post Comment
Interested in sponsoring a week of my RSS Feed? Click here to learn more.
Cancel Sign Up
Thank you for subscribing to our daily updates. We promise not to use your e-mail address for any other reason. You can unsubscribe at any time.


text:
About Us
Contact Us
Dignited Igniters Network
Our Team
How to Pitch your product or start-up to the Dignited
Privacy Policy
Terms of Use
MTN Uganda returns the famous "Juzza Internet" Promo that doubles your data when you buy a bundle
Facebook in partnership with Airtel plans to lay 500 miles of fiber cable in Uganda
How to extend your MiFi's WiFi signal using a strong WiFi router
Internet routers price comparison from Ugandan mobile networks
Tecno Camon CX(C10) and CX Air launch in Kenya; Here are the official specs and price
5 features developers will love about Android O, the latest Android version
Sony's new patent will make it possible to charge your phone wirelessly using your friend's
Tecno L9 Plus: Here are the specs, price and where to buy Tecno's L9 Plus
Why you should not buy a 3D TV in 2017
What we know about Startimes HD TVs with in-built decoders
GOtv Lite monthly subscription rates hiked by 3K, now at Shs. 8,000
DStv Explora Versus Explora 2: Here's what's new
Banking & Payments
Kenyans in the diaspora lead in sending Mobile Money back home
Ecobank's Masterpass QR is the smart move towards a cashless society
A complete list of Worldremit pickup locations in Kenya
Kenyans in the diaspora can now send money back home to bank account holders on KCB
personal computing
How to tweak your registry like a Pro and make Windows run faster
The difference between Micro USB and USB type C
Learning code in Uganda: These are the best options to get you started
Is your PC not recognising your Android smartphone? Here's how to fix it
Web & Social
SoBro is furniture that knows about your devices
A 2017 complete guide to online Shopping and Shipping websites in Uganda
Facebook messenger lite is lightweight version of Messenger that's faster and uses less data
Pilot is a smart language translation earpiece by Waverly Labs
SoBro is furniture that knows about your devices
A 2017 complete guide to online Shopping and Shipping websites in Uganda
Kenyans in the diaspora lead in sending Mobile Money back home
Ecobank's Masterpass QR is the smart move towards a cashless society
About Us
Our Team
Contact Us
Privacy Policy
Terms of Use
Search for:
smartphone prices
ZTE smartphone prices in Uganda and where to buy
Alcatel smartphone prices in Uganda: Your best option for low-end and mid-range devices
iPhone prices in Uganda: Here's how and where you can buy your next iPhone
Blackberry smartphones in Uganda: These are the best prices and deals we know of so far
HTC smartphone prices in Uganda: These are the latest HTC phones in the stores


text:
0 active jobs | Company Website
It all began when William R. Kelly—founder of the temporary staffing industry—established Kelly Services® in 1946. The company's traditional expertise started with office services, call center, light industrial, and electronic assembly staffing. Through the years, we expanded our expertise and established a proven record of successfully matching job seekers to opportunities in disciplines such as science, engineering, law, education, healthcare, IT, and finance. As a Fortune 500® company, Kelly® has evolved into a global workforce solutions leader, making employment connections for nearly half a million people around the world every year. Since our founding, we have embodied the true spirit of social responsibility. Our character and values resonate through our culture. Inherently—through our core business focus—we seek to improve the quality of life for our employees, their families, and their communities, as well as society at large. We embrace the role we play and the value we contribute to society—whether ensuring equal opportunity to employment, promoting safer workplace conditions, advocating for healthcare reform, or adhering to sustainable business practices. Together with our employees and our customers, we will continue to work toward ensuring that our legacy will remain strong for generations to come. For more than 60 years, people have trusted Kelly Services to help them reach their career goals. We are your proven link to employment opportunities that will pave the way for a successful future. Whoever and wherever you are—and with the right skills and attitude—Kelly can help you find a job, expand your career, and build your future.
Refine Search
For your privacy and protection, when applying to a job online:Never give your social security number to a prospective employer, provide credit card or bank account information, or perform any sort of monetary transaction. Learn More >>
By applying to a job using CareerBuilder.com you are agreeing to comply with and be subject to the CareerBuilder.com Terms and Conditions for use of our website. To use our website, you must agree with the Terms and Conditions and both meet and comply with their provisions.
0 Results
No Jobs Found.
This company also has jobs posted on the following Niche Sites: Sologig.com MiracleWorkers.com WorkInRetail.com
Or find more companies on our Company Search Page.


text:
Add a greeting card to your chocolate gift in checkout.
Order now and choose a future delivery date to suit you.
Standard p&p from just £3.95
'The best chocolate in the world' As featured in
Chocolate Trading Co
Welcome to Chocolate Trading Co. Sign in.Register with us
my basket (0 items)Total £0.00


text: For thousands of years, rings have been more than just adornment. They have been symbols of authority, symbols of promises, and even legally binding signatures. Today rings still speak volumes about you. Our Aquamarine Teen Rings are as eloquent as they are beautiful and always one of a kind. Choose Red Garnet and White Gold or Blue Sapphire and Yellow Gold: it's your choice. Mix and match gems and precious metals for a look that's exactly you. Our amazing array of designs by talented jewelry designers means you can find Aquamarine Teen Rings that you can't find anywhere else. Because all Gemvara Aquamarine Teen Rings are made from scratch to your specifications, we can hand-select the finest quality gems and recycled precious metals to perfectly suit your design. Aquamarine captures the icy beauty of the sea.When washing your hands, don't leave any rings on the rim of a sink where they can slip down the drain! Clean Aquamarine Teen Rings in a bowl of water with mild dish soap: use a soft brush where dust collects. Gemvara's exceptional heirloom-quality Aquamarine Teen Rings are designed to be passed down for generations, crafted by hand in the United States.


text:
Thermaltake's Level 10 enclosure Is the $700 Level 10 the ultimate enthusiast case, or is it the ultimate example of form over function? We investigate. Type: Feature article Tags: Cases |
28 |
Cheap MSI Fuzion motherboards officially launched You know those two affordable MSI motherboards with Lucid Hydra GPU load balancers we saw at Computex? Well, MSI has now formally launched... Type: News story Tags: Motherboards |
18 |
3D Vision Surround makes debut in beta GeForce driver What's geekier than sitting in front of a 3D monitor while wearing stereoscopic glasses? Sitting in front of three identical 3D monitors while while wearing... Type: News story Tags: Graphics |
60 |
Portege R700 oozes awesome from every port Like a junior-high boy who is always secretly on the prowl, I have been constantly on the lookout for my next ultraportable laptop, especially since... Type: News story Tags: Mobile computing |
117 |
Corsair's Force F120 solid-state drive The first SandForce-based SSDs only squeezed 100GB of storage capacity from 128GB worth of flash. Corsair's Force F120 kicks that capacity up to 120GB,... Type: Feature article Tags: Storage |
10 |
From start to finish in Rez HD This year's E3 expo has played host to all sorts of new gaming hotness. We've learned that the Need for Speed franchise will return... |
17 |
Crucial's RealSSD C300 solid-state drive Crucial's RealSSD C300 is the first SSD with a 6Gbps Serial ATA interface and ONFI 2.1 flash memory. Let's see how it compares. Type: Feature article Tags: Storage |
56 |
Upcoming MSI Lucid mobos to start at $125 When we benchmarked it last year, MSI's original Big Bang Fuzion motherboard left us with mixed feelings. The board and the integrated Lucid Hydra... Type: News story Tags: Motherboards |
16 |
Antec plans to get into the audio business Enthusiasts may associate the Antec name with power supplies, enclosures, fans, and those neat noise-dampening panels on P-series cases. But high-end computer speaker setups? Believe... Type: News story Tags: Multimedia |
57 |
Latest NZXT case has Star Wars vibe The folks at NZXT have parted with their previous stylistic influences with the new Phantom case, which we got a chance to see last week... Type: News story Tags: Cases |
17 |
Skulltrail spiritual successor sighted in EVGA suite Intel's Skulltrail platform may already be distant history for many enthusiasts. However, EVGA apparently intends to rejuvenate the concept with its Classified SR-2... Type: News story Tags: Motherboards Cases Power |
36 |
Thin CULV Core i5 laptop spotted in MSI booth COMPUTEX — Only a week after seeing Intel announce its 32-nm Consumer Ultra-Low Voltage processors, we were able to get up close to a... Type: News story Tags: Mobile computing |
21 |
Enermax, Seasonic show unique 80 Plus Gold PSUs COMPUTEX — Power supplies with 80 Plus Gold certification abounded at Computex this week, but some units stood out more than others. Two new introductions... Type: News story Tags: Power |
29 |
Biostar, MSI flaunt Sandy Bridge motherboards COMPUTEX — Intel's next-generation Sandy Bridge processors may not be due out for at least another six months, but here at Computex, some motherboard makers... Type: News story Tags: Motherboards |
17 |
We take a closer look at Asus' 10'' Eee Pad COMPUTEX — We visited Asus' booth yesterday for a closer peek at the 10" Eee Pad. (To see our coverage of the 12" Eee Pad... Type: News story Tags: Mobile computing Personal tech |
38 |
Thermaltake shows mechanical gaming keyboards COMPUTEX — Keyboards with clicky, mechanical keyswitches have gathered a cult following in recent years, and Thermaltake is planning to capitalize on that. Sitting next... Type: News story Tags: Input devices |
52 |
Kingston preps 64GB SD card, external USB 3.0 SSD COMPUTEX — At a small press conference in a Taipei hotel this morning, Kingston showed us two new storage devices: a 64GB flash card based... Type: News story Tags: Storage |
13 |
Gigabyte mobos support 1500W CPU power, overclocking via iPad COMPUTEX — The Gigabyte folks had more to offer at Computex this week than provocative predictions. At a press event yesterday, Gigabyte introduced its... Type: News story Tags: Motherboards |
31 |
Aorus gives its GTX 1070 the triple-fan treatment | 1 |


text:
Get volume discounts, fast shipping, and a dedicated account manager.
Hansgrohe, a German manufacturer of kitchen and bathroom plumbing fixtures, is known for bringing the art of bathroom design to life. With the help of world famous designers, Hansgrohe products are not only contemporary, but also designed for the future.
Founded in 1901, Hansgrohe's passion for innovation is one reason they've become the world's largest supplier of showerheads and hand-held showers. Kitchen and bathroom faucets round out Hansgrohe's extensive catalog of products.
Outfit your home with contemporary elegance from Hansgrohe. Just beginning your search? We recommend starting with our best-selling Hansgrohe PuraVida, Raindance, and Talis collections.


text: IMG_4055 April 6, 2016 Katherine Heigl I'm thrilled to share with you all of the things that inspire me, give me purpose, and bring beauty, peace and gratitude to my life. My blog represents my thoughts, interests and hobbies. I hope my ideas speak to you and inspire you the way countless others have done for me. No Comments Leave a Reply Cancel Reply Name * Email *


text:
Filter by: Content type: ALL Problems Articles Games Stage: All Stage 1&2 Stage 2&3 Stage 3&4 Stage 4&5 Challenge level:
Janine noticed, while studying some cube numbers, that if you take three consecutive whole numbers and multiply them together and then add the middle number of the three, you get the middle number. . . .
Pick a square within a multiplication square and add the numbers on each diagonal. What do you notice?
What happens to the perimeter of triangle ABC as the two smaller circles change size and roll around inside the bigger circle?
Take any pair of two digit numbers x=ab and y=cd where, without loss of generality, ab > cd . Form two 4 digit numbers r=abcd and s=cdab and calculate: {r^2 - s^2} /{x^2 - y^2}.
A serious but easily readable discussion of proof in mathematics with some amusing stories and some interesting examples.
A introduction to how patterns can be deceiving, and what is and is not a proof.
Helen made the conjecture that "every multiple of six has more factors than the two numbers either side of it". Is this conjecture true?
Think of a two digit number, reverse the digits, and add the numbers together. Something special happens...
Investigate sequences given by $a_n = \frac{1+a_{n-1}}{a_{n-2}}$ for different choices of the first two terms. Make a conjecture about the behaviour of these sequences. Can you prove your conjecture?
Can you find a rule which relates triangular numbers to square numbers?
Here is a machine with four coloured lights. Can you make two lights switch on at once? Three lights? All four lights?
Many numbers can be expressed as the difference of two perfect squares. What do you notice about the numbers you CANNOT make?
Can you find the values at the vertices when you know the values on the edges of these multiplication arithmagons?
Can you find a rule which connects consecutive triangular numbers?
Show that all pentagonal numbers are one third of a triangular number.
Problem solving is at the heart of the NRICH site. All the problems give learners opportunities to learn, develop or use mathematical concepts and skills. Read here for more information.
Show that for any triangle it is always possible to construct 3 touching circles with centres at the vertices. Is it possible to construct touching circles centred at the vertices of any polygon?
Take any whole number between 1 and 999, add the squares of the digits to get a new number. Make some conjectures about what happens in general.
In 15 years' time my age will be the square of my age 15 years ago. Can you work out my age, and when I had other special birthdays?
Explore the relationship between quadratic functions and their graphs.
Have a go at creating these images based on circles. What do you notice about the areas of the different sections?
Drawing a triangle is not always as easy as you might think!
Can you find some Pythagorean Triples where the two smaller numbers differ by 1?
Two semicircle sit on the diameter of a semicircle centre O of twice their radius. Lines through O divide the perimeter into two parts. What can you say about the lengths of these two parts?
Points D, E and F are on the the sides of triangle ABC. Circumcircles are drawn to the triangles ADE, BEF and CFD respectively. What do you notice about these three circumcircles?
Explore the relationship between simple linear functions and their graphs.
Alison has created two mappings. Can you figure out what they do? What questions do they prompt you to ask?
This article for teachers discusses examples of problems in which there is no obvious method but in which children can be encouraged to think deeply about the context and extend their ability to. . . .
The points P, Q, R and S are the midpoints of the edges of a non-convex quadrilateral.What do you notice about the quadrilateral PQRS and its area?
Charlie has created a mapping. Can you figure out what it does? What questions does it prompt you to ask?
Four rods of equal length are hinged at their endpoints to form a rhombus. The diagonals meet at X. One edge is fixed, the opposite edge is allowed to move in the plane. Describe the locus of. . . .
Find out about Magic Squares in this article written for students. Why are they magic?!
The points P, Q, R and S are the midpoints of the edges of a convex quadrilateral. What do you notice about the quadrilateral PQRS as the convex quadrilateral changes?
Do you notice anything about the solutions when you add and/or subtract consecutive negative numbers?
Use the computer to model an epidemic. Try out public health policies to control the spread of the epidemic, to minimise the number of sick days and deaths.


text:
USA - English
Canada - English
Canada - Français
BROAN offers the widest variety of ventilation fans in the industry. For the latest high-performance, ENERGY STAR® Certified solution to today's green building requirements, or ultra quiet operation for a comfortable environment, BROAN offers stylish options to fit any situation from a retrofit to a new build out. As the leader in residential ventilation, we will confidently meet your needs.
The leader in home ventilation, BROAN offers the broadest line of kitchen ventilation products in North America, providing choices, style, value and a name you can trust.
As the industry's leader in providing Fresh Air Systems for residential construction, BROAN sets the bar in standards of innovation and excellence. We are continually seeking ways to provide the best quality, technology, style and care to our customers.
Air in your attic can reach 150°F during the summer, warming your home, increasing the load on your air conditioner, and adding to your energy bill. In winter, ice dams can result from poor ventilation, and moist air in the home can condense on attic surfaces causing mildew, rotting wood and wet insulation. BROAN has the solution!
It's quite simple. You may use some rooms only briefly, such as the bathroom, as you get ready for work in the morning. Heating the entire home for this purpose is pointless. Instead, opt for spot heating, which will effectively heat the area you need – when you need it.
This range hood can work in an ADA-compliant application. See Additional Information above.
Cleaning a Range Hood Filter – Danny Lipford
Proper Use of a Range Hood- Danny Lipford
How to Ventilate the Whole House
Proper Ventilation Equals A Healthy Home - Danny Lipford
What are CFMs? - Danny Lipford
Range Hood & Ventilation Fans Tip - Check Terminations of Ventilation Ducts
How do I determine the size of the range hood I need? - Danny Lipford
Is there any regular maintenance needed on my range hood? - Danny Lipford
Does an Over the Range microwave provide adequate Capture? - Danny Lipford
Give Your Kitchen a 72-Hour Facelift with Danny Lipford
Island Range Hoods for Kitchen Upgrades - Danny Lipford
Importance of a Range Hood for Indoor Air Quality
Kitchen Renovation Includes Adding Range Hood with Danny Lipford
Keep your kitchen clear of smoke and fumes. Don't neglect ventilation when remodeling your kitchen, ConsumerReports.org
Cook with a gas stove? You could be breathing polluted air, study says, Los Angeles Times
Using gas stoves with no hood may expose many to air pollution, UPI Health News
Breathe Easy – What you need to know to ensure proper home ventilation, K+BB
5 Kitchen Renovation Goofs To Avoid, Consumer Reports
Clearing the Air, K+BB
The Kitchen as a Pollution Hazard
Fixing Stove Hoods To Keep Pollution Out Of The Kitchen
Take a few minutes and tell us about your experience on our Web Site.


text:
Hyde Sofa
- Summary
Sleek and stylish, Hyde brings roomy comfort to smaller rooms and more intimate furniture groupings. Shelter arms, tapered legs and loose back and seat cushions give it modern flair, while quality craftsmanship ensures heirloom durability.
- Overall: 86"W x 37"D x 33"H
- Polished-nickel nailhead trim.
- Handcrafted in the USA by skilled artisans. American and imported materials.
- Frame crafted of kiln-dried sustainably harvested hardwood.
- Sinuous steel springs in seat and back.
- Seat cushions have high-resiliency foam cores with up to 10% soy-based materials, wrapped in either our premium down blend or our thick, double layer poly fiber.
- Reinforced with double-dowels and corner blocks.
- Ebony-stained solid maple feet.
- Available made to order in all of our custom upholstery fabrics; to place a custom order, please call 888-922-4108 for assistance from one of our furniture specialists.
- Part of the Williams Sonoma Green Collection.
- Available in many of our custom fabrics.
- Furniture upholstered in mohair will not have welting (piping) or topstitching.
- 6-8 week in-home delivery with White Glove service.
- View the complete collection here.
This item is part of our Green Collection. Our Green Collection creates truly great design with an eco-friendly point of view. These Green products are distinguished by any one of the following characteristics: Organic, Re-cycled, Re-claimed, Sustainable.
- Dimensions & More Info
- Overall: 86"W x 37"D x 33"H
- Seat width: 78"
- Seat depth: 20"
- Seat height: 19"
- Seat interior: 78"W x 20"D x 19"H
- Diagonal depth: 32"
- Arms: 4"W x 31"H
- Legs (fixed): 5.75"H
- Weight: 121 lb.
- Use & Care
Create your own custom-upholstered furniture using the fabric of your choice. For more details, please call 1.888.922.4108.
Furniture upholstered in mohair will not have welting (piping) or topstitching.
- Clean with a soft dry or slightly damp cloth; dry thoroughly.
- Avoid harsh cleaners (including ammonia-based glass cleaners) and abrasives as they will damage the finish.
- Brush or vacuum regularly (use a nonmetallic stiff-bristle brush).
- Protect from direct sunlight to prevent fading.
- Dab spills immediately with a soft white cloth dampened with distilled water (not tap water). Test fabric cleaners first in an inconspicuous area. Do not saturate fabric with cleaner. Pat instead of rub to avoid altering texture. Clean stain from outer edge to prevent "circling."
- Thorough cleaning is recommended every 12 to 24 months. Do not wait for severe soiling. We recommend a professional in-home upholstery cleaning service.
- Do not remove cushion casings for cleaning. Unlike slipcovers, upholstered casings are not meant to be removed.
- Never use detergents, polishes, preservers or saddle soap. They can remove natural oils.
- Dust or vacuum for routine maintenance.
- Do not place in direct sunlight; it will lighten or discolor leather over time.
- Dab spills immediately with a soft white cloth dampened in distilled water (not tap water). If spill penetrates leather, use lukewarm distilled water and a soft white cloth to massage stain and surrounding area. Blot with a dry cloth and allow leather to air dry.
- Most surface scratches will fade over time if you rub them with your fingertips. Friction and heat from rubbing helps to redistribute wax in surrounding areas to cover the scratch. Not all scratches have the same depth, and this may not work on deeper scratches.
Composition & Construction
- Our leather upholstered products are constructed from aniline leather. Only the top 5% of hides can be made into aniline leather, making it the finest available. The full-grain or top-grain leathers are soaked in transparent nontoxic dye, with no subsequent pigmented finish coats applied. Dye penetrates the hides, letting the natural grain show through. Our ivory leather, white pebbled leather and white faux ostrich are exceptions to this process; they are semi-aniline dyed, with a layer of pigment applied afterwards.
- Anilines have natural markings that vary from hide to hide. They also have color variations due to the leather's ability to accept dyes differently in various areas of a hide. Markings and shading distinguish genuine leather from man-made materials. Appreciating shade differences among hides also means appreciating them on individual furniture pieces, because no two pieces can be produced from a single hide.
- Our leathers appeal to customers who find the natural color variations, markings and patina of pure aniline leathers unique and beautiful. They may not appeal to customers who want uniform color and surface commonly seen on less expensive leathers that are heavily corrected and pigmented.
- Shipping
Shipping Information
All custom upholstery items will be shipped directly from the manufacturer and are not eligible for rush shipping. Please allow 6 to 8 weeks for delivery. Item arrives fully assembled and in one piece by our in-home White-Glove Delivery service. For White Glove items, we'll call you in advance to schedule a delivery time, then bring the item into your home, unpack and inspect it, perform simple assembly and dispose of all packaging material. Complete beds will be set up; headboards only will be unpacked, but not attached to existing bed frames.) Delivery is by appointment, Monday – Saturday; our delivery service will call to arrange a day. Available in the 48 contiguous states. Please call 888.922.4108 for additional assistance.
Shipping Rates
The charge for all shipping is included in the flat-rate delivery fee listed below the item's price
Return Policy
Our products are designed with your comfort and style in mind.
Made to Order and Final Sale Furniture items are non-returnable, and cannot be cancelled once the order is placed.
Monogrammed orders cannot be cancelled or returned.
Final sale items ending in .97 or .99 cannot be returned.
Should your item arrive damaged or with a defect, please contact us at 888.922.4108 within 7 days so that we may arrange for a replacement. Frames and springs are warranted to be free of manufacturing defects for the product's life, subject to the following conditions: warranty applies only to original owner and normal residential use. Defects resulting from negligence, abuse or use in a commercial, hospitality or other non-residential setting are not covered. For warranty service, call 888.922.4108.
All other items (with the exception of those marked as Final Sale) may be returned within 30 days of receipt for a refund of merchandise only. View Full Return Policy.
- Request Swatches
Click here to request a swatch. There is no fee or shipping charge for swatch requests. You can expect to receive your swatches within seven to ten business days.


text:
At the top of the CDC's list of primary risk factors for all chronic diseases are: smoking, poor nutrition, and sedentary lifestyle.
Living a healthier lifestyle can help to prevent heart disease. This includes the following:
Eliminate all tobacco products |
You should be aware that all tobacco products are included as risk factors for chronic illness, not just cigarettes. And, although there may be medical uses from derivatives of some social drugs, such as alcohol, there is no therapeutic use for nicotine. As soon as you stop smoking, your body begins to heal itself from the devastating effects of tobacco. |
Adhere to a heart-healthy diet |
One aspect of managing your heart attack risk factors includes eating a heart-healthy diet that includes appropriate levels of the following:
To try to eliminate any confusion, the federal government has established a food plate and food labeling laws. The food plate can help you eat a variety of foods while encouraging the right amount of calories and fat. To find more information about the Dietary Guidelines for Americans 2010 and to determine the appropriate dietary recommendations for your age, sex, and physical activity level, visit the Online Resources page for the links to the ChooseMyPlate.gov and 2010 Dietary Guidelines sites. Please note that My Plate is designed for people over the age of 2 who do not have chronic health conditions. Maintaining a heart-healthy, balanced diet will help to:
Follow an appropriate exercise program |
One vital step toward reducing your chances of having a heart attack is making the time to exercise. Today, with our fast-paced society, people must schedule time to exercise. Choose an activity that you enjoy doing, then talk with your health care provider about an exercise plan that meets your individual capabilities and needs. An exercise program will help in the management of almost all stroke and heart attack risk factors. The Dietary Guidelines for Americans 2010 recommend that an individual engage in at least 30 minutes of moderate-intensity physical activity, above usual activity, at work or home on most days of the week. Regular physical activity will help to promote health, psychological well-being, and a healthy body weight. Always consult your health care provider regarding your healthy diet and exercise requirements. |
Click here to view the
Online Resources of Women's Health


text:
Attend CICS training in Olathe. NetCom Learning provides more than 1500+ IT and Business training courses throughout Olathe, KS and the country. Whether you're looking for customized onsite cics training for a private group throughout Olathe area or a public instructor-led online cics class, NetCom Learning has the solution for you. IBM CICS (Customer Information Control System) is a family of application servers and connectors that provides industrial-strength, online transaction management and connectivity for mission-critical applications. |
Private training is an excellent corporate solution to achieve maximum value for your training funds. Private group training is ideal for situations requiring more than 1 student taking the same training. NetCom Learning has been helping our clients achieve their private group training objectives since 1998. Our goal is to meet your learning requirements, with a strong focus on achieving excellence in all your interactions with us.
We can deliver any classroom or online course in a private format - at your facility, ours or online, in any time zone and anywhere in the world.
Since students in your group have similar goals, we can customize and streamline your training program, which means a more relevant education and often enables us to discount the training by 10-30% or more.
Discover your exact savings with a Free Private Training Consultation today! Your dedicated NetCom Learning Consultant will determine your needs, answer your questions and present you with a complimentary quote for your private training program.
Request Free Private Training Consultation »


text:
The article you have requested: Gaming Today Impressions of Dark Sector (Xbox 360) is not available. Perhaps you would like to post an article to share news with our community or explore:
We recommend you return to the article list and browse the links from there. If the problem persists, contact us with the details.