text:
Thanks to 'Techsan' for editing this story.
Where do I begin? I guess when I was just a young buck of nineteen freshly out of high school on his way to college. Like a lot of kids at that time I guess I thought I was hot stuff. I dated a lot and tried to see how many pairs of panties I could get into. God, I loved the challenge. Will she or won't she? That is the question. I had as many wills as I did will nots.
I started college but like most young guys at that time I dropped out eventually. My mind was on the pussy challenge, not studying. I did learn enough in college to land some good jobs.
I was in sales which put me in a lot of homes. Damn, I like that. Most places I stopped were just for business and I took care of it, being one of the top salesmen. Then there were the pussy stops. That's when you could smell it upon entering a residence. The woman, usually alone, dressed in sexy clothes or robes and nightgowns. In these cases I usually got the sale quickly and then did what comes natural with a young buck and a lonely or horny woman of any age.
The hardest thing to judge was determining if the woman was flirting or wanted sex. I didn't want to lose my job for a piece of ass when there was so much out there. I usually let the ladies take the lead. If they brought up sex or made the first move I jumped their bones. I probably passed up a lot of pussy just to be on the safe side.
I eventually met a really nice gal when I was doing a little shopping at the mall. She was just a sweet little thing standing a little over five feet tall, a big bubbly smile and big boobs. As she waited on me I used my charm and tried to get a date. After about the tenth time coming in the store she agreed to go out with me. I was really falling for this girl. I was just hoping she was doing the same.
I still kept my sales job while dating Nancy. We got along great together and eventually made love. Yes, it was love. It was so much different than the sex I was used to. I wanted to please this woman, not just myself. After about ten months of dating, she became pregnant. She was on birth control but I guess we were that one in ninety-nine chance.
In her fourth month of pregnancy we got married. Looking back I guess I wasn't ready to be married. I loved Nancy, I really did, but I was twenty and pretty immature. I thought with my dick. I had sex with a few gals after getting married just because I could. It was different from the loving sex I had with Nancy. More than anything I still believe it was the chase. Just to say I was able to get into a girl's pants and then do it.
I didn't brag about it to anyone but I believe people knew. I was a married guy with a kid on the way. I know people want to call me a bastard, prick, lowlife, and all the other adjectives. I deserve to be called them all. I know that now but then it was just trying to get into any woman's panties. I was an immature kid.
One day Nancy found out I banged one of the girls at the office. She cried and cried and we talked about it. She thought about divorcing me and probably should have. She eventually forgave me. I really began feeling bad about the affair. For some reason at twenty years old I didn't think about my actions until it was too late. Then after I found out what it did to my loving wife, it tore me apart. I vowed never to cheat on her again if she would forgive me.
I kept that promise and tried to be a good husband. The baby was born, a son we named Jacob. We were now a family. We had our problems like any family. We could always use more money. We didn't agree on what to buy. Like any young couple we had a big difference of opinions. But we were able to work them out.
One thing was for sure and that was that after my affairs my love grew for Nancy. I might not have always showed it but it was there. I would die for that woman. Making love to her was fantastic. I believe it was because of the effort both of us put into it. I would do anything to make her climax. To this day, hers is the only pussy I ever ate. I loved sticking my tongue deep into her pussy until she climaxed.
We even tried anal sex about three times. It wasn't all it was hiked up to be. Her ass was so tight it hurt my dick. I used some KY jelly so I could at least get the head in. It did feel good shooting a load in her ass though. She preferred good old-fashioned pussy sex. Didn't make any difference who was on top or if I got her from behind, it was all good. She didn't like to give head. She tried it a few times but I could tell she hated it. I wasn't going to make her do it when she didn't received pleasure from it. I did continue to eat her out. I know it made her feel great.
A couple of years later we had another son, David. We couldn't have been happier. We were truly a family. Probably as normal as they come.
I always wanted to own my own business. To be able to control my own destiny. Nancy and I talked and talked about it. Money was kind of tight with having the two boys and all the other expenses. We finally found a small hardware store. We bought it on land contract and made the business a success. Not a lot of money but enough to survive. I was really happy to have fulfilled that dream of having my own business. A wonderful wife and two sons. Life was good.
Nancy didn't lose all her weight after the second baby. She always talked down about herself. I told her she looked fine. I loved her heavy, skinny, it didn't make any difference to me. Of course she never really believed me but it was true. When I looked at her I didn't think about her weight. Granted that a beautiful sculptured body looks great but I loved Nancy regardless of the weight factor. Besides she was a little overweight, not obese. Hell, I was thirty pounds overweight myself. She would go on diet after diet. Always trying something to take the weight off.
We would argue about it over and over again. She always ended the arguments by throwing the affair up at me even though it was years and years earlier. I guess even though she said she forgave me deep down the negative feelings were still there.
She worked part time in her job while the boys were growing up. After the boys were in high school, she took a promotion in her job. She was a department manager and would make a lot more money. She decided to get back on the diet kick and also joined a health club for women. I didn't mind this since it was an all woman exercise place. Whenever a woman joins a health club and the husband doesn't, it seems to lead to affairs.
I was surprised she stayed with it. She began to lose weight and was feeling good about herself. That made me feel good. I liked her to be happy and if being thinner made her happy then I was happy. Our sex life started to rear up again. Like most married couples it waned for a few years while being busy with the family. Whenever we made love it was good. I guess after so many years of marriage you just sort of take the sex for granted. Sometimes we might go as much as two months without having sex. But then when we made love it was great.
She liked her job and new found body. She just seemed so much happier. Then it happened. She came home and asked if I would mind if she went out with her friends. I said of course not, you've been going out with them for years. She mentioned they were having a girls night out on Saturday and wanted to make sure I didn't have a problem with it. This just seemed strange. I knew something was bothering her. So I asked her what the problem was.
She just said she was probably going through a menopause phase and her mood swings were probably getting to her. I hugged her and said I loved her and I would always be here for her. She just hugged me back. I felt her shudder a little. I knew we had a problem but it wasn't the right time to face it. I would just have to think about it and decide how to approach her.
On Saturday I watched to see how she dressed to go out with the girls. She just wore a pair of black slacks and a nice blouse. She looked good but to me she always looked good and I often told her so.
She told me where they were expecting to go in case I needed her for anything. Then her friend Pam picked her up and they left. It was bothering me. I had to know what was happening to her. I waited a few minutes and headed out myself. I really didn't want to spy on her because I always trusted her. There just seemed to be something wrong with her demeanor. I had to find out what it was.
Nancy and her friends went to a combination dance hall-lounge-night club. It was really big. It was easy to get lost in the crowd. I went up to the second floor on kind of a balcony where I could over see the lower level. I was able to spot Nancy and her girlfriends sitting together in two of the half round booths. They seemed to be having a good time. Maybe I was wrong, maybe this wasn't part of the problem. I ordered a drink and just kind of watched Nancy. I felt bad being there but I needed to know for my own well being.
Nancy's friends were a mixture of married, single and divorced women. Most I have met at one time or another. There were a few there that I didn't recognize. I figured she met them at the health club. After about a half hour a few guys came over and asked some of the girls to dance. The first couple of times Nancy shook her head 'no' but after she had a few drinks she agreed to dance a little. I really didn't have a big problem with her dancing but because of the possible problem she might be having, it made me nervous.
She was starting to spend more and more time on the dance floor. I didn't recognize any of the guys. They could have been from work or just strangers out for a good time. I decided to stay for a little while longer to see what was going on. She started to dance a few slow songs with one guy and he was holding her close. I saw him slide his hands down to her ass and pull her toward him. She just reached back and gently pushed his hands away. He did it a number of times and she continued to just push it away. The last time he grabbed her ass she left it there awhile longer before pushing it away. Then I saw him reach up and touch her breast. He held it there a few seconds and then she again pushed his hand away.
It was bothering me. Should I say or do something? Should I confront her in some way? She wasn't suppose to know I was even here. I was wondering if this went on before or maybe she just didn't want to make a scene. I just didn't know. It wasn't like her to do nothing about it After the song ended she went back and sat with her friends. I was too far away to hear any conversations but some of the girls were laughing or smiling. It could have been about anything.
A few minutes later the girls were finishing their drinks and were getting ready to leave. They were saying goodbye to the other girls and the guys. The guy Nancy was dancing with reached over and gave her a light goodbye kiss. Nothing special, just a light kiss, but it really bothered me. Was I reading more into this than I should have? I know I will never forget it no matter how innocent it was.
I got up quickly and left out the back door, got into my car and headed home. The boys were already in their room watching TV. I just said hi to them and went and made myself a drink. I already had liquor on my breath and figured it would be a good cover.
Nancy arrived within ten minutes and I greeted her. I asked her if she had a good time and she said she did but she seemed a bit nervous. I looked her in the eyes and said, "Nancy, do we need to talk?"
To the best of my knowledge Nancy has never lied to me. I'm sure she has secrets like everyone else but I have never caught her in a bold lie. I could see tears form in the corner of her eyes. She looked at me and said, "Jim, I have a problem and I don't know what to do." and she started crying.
I held her close and hugged her and I asked her if it had anything to do with her girls night out?
She said, "Yes. . .No. . .Maybe. . .I don't know? Let's sit down, I think we need to talk."
I said, "Nancy, tell me why you are so upset. Start with this evening, what happened?" I wanted her to tell me about tonight because I saw what was happening and could guage her truthfulness.
"Well," she said, "We arrived at the club and were drinking. I started dancing with a few guys. One in particular. He was touching my butt and breasts and I should have slapped him but didn't. I let him feel me up and didn't stop him. I pushed his hands away and when he put them back I really didn't get mad."
"Nancy, why didn't you stop him? Push him away or something."
She was crying lightly and said, "I liked it, Jim. I wanted him to touch me. I'm so sorry, I can't get these feeling out of my system. It's making me crazy."
"Was this the first time it ever happened, Nancy?"
She started crying again and said, "No, this has been happening the last couple of months and I can't shake it. I want to be touched. I want men to want me."
"MY God, Nancy, have you been having affairs? Have you been fucking other men? Have you been cheating on me?"
She was crying and answering me at the same time, saying, "God, no, Jim, I haven't ever cheated on you. I have never had another man. Never but as of late I have let men touch me. I was hoping it was just a short phase I was going through and you would never find out. I just can't hide it anymore. It's tearing me up inside. I can't live like this. I had to tell you. Jim, I love you so much but I feel like I missed something. You're the only man I've been with. I know you have had other women."
"Stop right there, Nancy. You have thrown that affair up to me for the last 15 years. You are not going to use it against me now to have an affair. Is that what you want, Nancy? My permission to cheat on me, to cuckold me, to make a wimp of me? It isn't going to happen Nancy. I love you with all my heart. I always will but you will not make a fool of me just so you can have some strange fucks. Someone to compare me to."
"Well, Nancy, is that what you want? A chance to have your little flings and then come back home to me after your pussy gets satisfied?"
Nancy replied, "I don't know Jim, I just don't know" as she was crying.
I had tears in my eyes as I told Nancy that she was right. Neither of us could live like this. We both needed time to think. I told her I was going to separate from her for approximately two months. She could live in the house with our sons. I would live in the apartment above my hardware store.
She said, "Jim, I love you. I don't want you to go. I'm just all mixed up. Please don't leave me."
"Two months, Nancy, two months to decide if you want your freedom and new found sex libido or if you want to stay married to me. I have no idea what you are going to do but if you decide to be a fuck slut for anyone I'll apply for a divorce immediately. This two months is for you to decide what you really want, not to see how many affairs you can get in. I will just take it 'A day at a time' and suggest you do the same."
"Since tomorrow is Sunday I'll gather my personal things and take them to the apartment. I'll also talk to the boys in the morning."
The next morning I told Jacob and David to come into the living room. I think they heard some of the conversation from their room the night before. They sat down as I explained that their mother and I were having some marital difficulties and that I would be living above the store till we got things straightened out. I explained that this was not their doing but was a problem between their mother and I. Nancy was standing behind them with tears in her eyes but not saying a word.
I explained to them that we both loved them very much and that they could talk to either of us at any time. I would still attend their school events. Nothing has changed other than I wouldn't be living at the house. No matter what the future brings they will always have a roof over their heads and won't have to worry about moving and losing their friends.
They wanted to know more but I told them that was all I could tell them for now. I said that their mother and I had some major problems to work out. We would know within two months what the future holds.
My boys were sad as they helped me take my personal stuff to the apartment above the store. I told them everything would be alright and for them to help their mother as much as possible. She needed their love if we were to stay a family. I instructed them to not ask her questions about the separation and help her around the house. They were good boys and I knew they would do what they could. I was only a mile away if they needed me.
Nancy needed time to work things out. I was in a world of turmoil myself. What if I lose her? My life was beginning to turn to shit. What could I do? What could I do? I guess it was all up to Nancy now.
End of Ch.1
Comments welcome
Thank you for reading my story.
DG Hear
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Comments - by Tootight112/07/16
good story
you bet. I wont go to ch 2 or 3, because we are reading about stupid kids that should have never gotten married to begin with. Do they love each other I would say yes, but so stupid, so immature, it'smore...
by Anonymous09/05/16I agree with TooTight1
The wife told him about the problem she's having. Told him she didn't know what to do, but she had NOT cheated on him - yet. Told him the problem wasn't going away and it was driving her crazy. Themore...
by xtchr07/24/16Don't Believe In Separation!
I just don't believe in separations. Either stay and really try to work it out or divorce. You can't work on problems if you are not together. Can't wait for the next chapter.
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text:
09 November 2007
Twin Stacks, SOLD
click here to bid on eBay
Twin Stacks, oil on linen panel, 7"x5"
Sometimes life just presents itself to the artist.
Observe and let yourself get inspired.
It is all there for the taking. How can one not want to capture
the color of this evening. The warm glow against the azure sky.
Daily painting,
oil painting,
Urban Landscape
3 comments:
- Sheila Vaughan said...
Woah! Stephen - this is a celebration and a half this is. Just such a powerful statement. Superb. And that red and blue is so difficult to get just right (I know from bitter experience) but you have it totally balanced here. Wonderful painting.
- 9.11.07
- Jason Waskey said...
The sentiment captured in this post, in your paintings--- all over this blog-- have inspired me to look with new eyes at the world around me.
I look forward to each painting as it arrives in my inbox.
Thanks so much! - 10.11.07
- Steven said...
Fantastic painting. Woo hoo!
- 19.11.07
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subset: commoncrawl


text:
Tuesday, September 20, 2011
oh hey....
kinda busy this week but, check out these beautiful, thoughtful, inspirational images shot by photographer, Pia Ulin There's something to love in each image whether it's herringbone floors, clean lined cabinetry, classic modern design juxtaposed against antiquity, a shot of color in a flatly lit room, a palette that looks so ripe you want to bite into it.......see if you can find that one detail that makes the shot.....
2 comments:
- Adie Andrews said...
What an incredible combination of different styles each detail which is related with certain style so perfectly fits to the whole conception. Just wonderful.
Greets holiday apartments in london - Wednesday, September 21, 2011 at 4:43:00 AM PDT
the light.
dee - Wednesday, September 21, 2011 at 11:40:00 AM PDT
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the B to the Log
Sep 2011
- People and their invented histories.
- blah, blah, blah
- Modwalls
- weekend up date
- These guys [ Johnny Tapete in Germany ] have...
- peace out
- bruised indigo
- taped
- guh! I'm going through photos of bathrooms f...
- oh hey....
- peace out
- ephemera
- La mac daddy: Lacanche
- toyboatoyboatoyboatoyboatoyboat
- missoni madness.... and a new toaster
- peace out
- $175k will get you this sw...
- mahna mahna
- Ikea in the 60s
- kindred spirits at the front
- Stuff to do, now that your kids are back in school...
- get your jollies
- The NYC, floating, public pool.
- You Are What You Do.
- Mixed Tape Monday
- peace out
- color blocking
Sep 2011
it's all about me, really.
- beachbungalow8
- Manhattan Beach, California, United States
- editor | interior designer | podcast personality | social media pollinator


text:
Time Warner Cable Aurora New York, Time Warner Cable Bundle Deals are a great answer getting all of your needs met with one company. With Time Warner Cable® bundles that include Digital TV, Road Runner® Broadband and Digital Home Phone, you'll be equipped to make the most of your day! They provide the best solutions for Digital Home Phone, Digital TV, and Road Runner® Broadband. Time Warner Cable® offers a lot more than just great cable television, Digital Home Phone service and Road Runner® Broadband.
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Include Time Warner Cable Digital Home Phone Service along with your Digital TV and Road Runner® Broadband to complete your telecommunications needs. Time Warner Cable New York One of the great things about Time Warner Cable Digital Home Phone plan is that you don't have to change your existing telephone number. We encourage you to compare the benefits you receive from Time Warner Cable Digital Home Phone with those you are receiving from your local telephone service provider.


text:
Journal article Open Access
Fuchs, Christian
{ "DOI": "10.1016/j.tele.2016.10.003", "abstract": "
Preprint version
\n\n
Fuchs, Christian. 2017. Sustainability and community networks. Telematics and Informatics 34 (2): 628-639.
\nDOI: http://dx.doi.org/10.1016/j.tele.2016.10.003
\n\n
Full article available at:
\nhttp://www.sciencedirect.com/science/article/pii/S0736585316303203
\n\n
Sustainability and Community Networks
\n\n
Christian Fuchs
\nUniversity of Westminster
\nLondon, UK
\nWestminster Institute for Advanced Studies (WIAS) & Communication and Media Research Institute (CAMRI)
\nhttp://fuchs.uti.at
\firstname.lastname@example.com
\n\n
Abstract
\n\n
Community networks are IP-based computer networks that are operated by a community as a common good. In Europe, the most well-known community networks are Guifi in Catalonia, Freifunk in Berlin, Ninux in Italy, Funkfeuer in Vienna and the Athens Wireless Metropolitan Network in Greece. This paper deals with community networks as alternative forms of Internet access and alternative infrastructures and asks: What does sustainability and unsustainability mean in the context of community networks? What advantages do such networks have over conventional forms of Internet access and infrastructure provided by large telecommunications corporations? In addition what disadvantages do they face at the same time? This article provides a framework for thinking dialectically about the un/sustainability of community networks. It provides a framework of practical questions that can be asked when assessing power structures in the context of Internet infrastructures and access. It presents an overview of environmental, economic, political and cultural contradictions that community networks may face as well as a typology of questions that can be asked in order to identify such contradictions.
\n\n
Acknowledgement: The research presented in this paper was conducted with funding provided by the EU Horizon 2020 project netCommons: Network Infrastructure as Commons, http://netcommons.eu/, grant agreement number: 688768
", "author": [ { "family": "Fuchs, Christian" } ], "container_title": "Telematics and Informatics", "id": "163751", "issue": "2", "issued": { "date-parts": [ [ 2016, 10, 29 ] ] }, "note": "Acknowledgement: The research presented in this paper was conducted with funding provided by the EU Horizon 2020 project netCommons: Network Infrastructure as Commons, http://netcommons.eu/, grant agreement number: 688768", "page": "628-639", "title": "Sustainability and community networks", "type": "article-journal", "volume": "34" }


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The rules PM me a pic Links to previous threads: The first one, April 28 - May 5, May 6 - 12, May 12 - 18, May 19 - 26, May 23 - 26, May 26 - 30, May 30 - June 2, June 2 - 6, June 6 - 9, June 9 - 13, June 13 - 16, July 16 - 20, June 30, July 6, July 19, July 26 I'm back with more pics. And with the power of the AP wire at my fingertips, the pics should be fabulous!
are they still making this stuff? I used to drink it instead of cokes & other soft drinks, but can't seem to find it anywhere anymore...
"People of Canada, earlier today the Army of the United States of America crossed our southern boarder in search of maple syrup and Labatt Blue. After several hours of fighting the Army of Canada was defeated and quickly surrendered. Behind me is the flag of your new rulers."
American troops that receive more than the 2 week training in the Army do thier extensive training in Quebec and Alberta. Our normal Rangers are equivalent to your Navy SEALs. Just food for thought.
CANADIAN POLICE TRAINING "Please watch as I demonstrate how to check a cat for concealed narcotics..."


text:
Rain 45°37° |
Sunrise: 06:40:18 am
Sunset: 07:15:18 pm
Moonrise: 07:15:59 am
Moonset: 08:09:36 pm Day: Cloudy, Showers Night: Scattered Showers; areas of denser fog. |
Mostly Cloudy 54°37° |
Sunrise: 06:38:28 am
Sunset: 07:16:32 pm
Moonrise: 07:50:33 am
Moonset: 09:23:33 pm Day: Mostly cloudy.Areas of dense fog in the morning. Night: Mainly clear early, then a few clouds later on. Low near 35F. Winds S at 5 to 10 mph. |
Partly Cloudy 62°40° |
Sunrise: 06:36:38 am
Sunset: 07:17:46 pm
Moonrise: 08:27:24 am
Moonset: 10:36:20 pm Day: Partly Cloudy Night: Becoming cloudy. |
Scattered Showers 58°38° |
Sunrise: 06:34:49 am
Sunset: 07:18:59 pm
Moonrise: 09:08:57 am
Moonset: 11:47:52 pm Day: Scattered Showers Night: |
Mostly Cloudy 55°37° |
Sunrise: 06:33:00 am
Sunset: 07:20:13 pm
Moonrise: 09:55:17 am
Moonset: 12:01:00 am Day: Mostly Cloudy; Isolated showers possible. Night: |
Partly Cloudy 62°38° |
Sunrise: 06:31:11 am
Sunset: 07:21:26 pm
Moonrise: 10:47:08 am
Moonset: 12:54:33 am Day: Partly Cloudy Night: |
Partly Cloudy 63°44° |
Sunrise: 06:29:23 am
Sunset: 07:22:40 pm
Moonrise: 11:44:56 am
Moonset: 01:55:28 am Day: Partly Cloudy Night: |
Rainy Days and Tuesdays!
Our next storm system arrives late tonight and Tuesday. Although the main part of the storm will be in the southern Rockies, a strong piece of energy will rotate north into KOTA Territory, bringing fairly widespread showers. Computer models have been very bullish with rainfall totals from this system, but at this time it appears as though much of the area could see .50" to 1.00" in Rapid City of much-needed rainfall. Snow will be confined to the highest elevations of the Black Hills and Big Horns. The rain ends Tuesday evening
We start the day Wednesday with fog, which could slow down travel in the morning. Then skies clear Wednesday afternoon and we warm up into Thursday. We are tracking another storm that has potential for Friday or even Saturday - and once again, mainly rain.


text:
The Small Town Catholic
A Blog about being a Roman Catholic in small town Wisconsin
Tuesday, September 25, 2012
Hail, or Maybe, "Fail" Mary Pass
It's all over the local news. As living only 20 minutes away from Lambeau Field, I am a HUGE Green Bay Packer's fan. The Packer's "loss" to the Seattle Sea Hawks is the biggest controversy in this whole brew ha ha with the referee lock outs. Read more
I figured I could post this since it was a Hail Mary pass! I meant no offense to our Blessed Lady by the title.
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Unustasid parooli?
Dreams So Real
Dreams So Real were a significant band in the Athens, Georgia music scene, gaining some national exposure in the late 1980s and early 1990s, including releases on Arista Records.
The trio consisted of chief songwriter Barry Marler on lead vocals and guitar, Trent Allen on backing vocals and bass, and Drew Worsham on drums. Forming in 1983 when its members met in an Athens record store, their debut single, "Everywhere Girl", came out in 1985 and was produced by Peter Buck of R.E.M.. Buck also produced their first full-length album, 1986's Father's House.
Read more about Dreams So Real on Last.fm
Minu paarilised
Kasutajad kellele meeldib "Dreams So Real"
24, Kaunas, Leedu
Näita veel inimesi
Lisa mind!
Sind näevad 1561 noormeest


text:
Sweet peas grace the flowerbed with delicate blooms in rainbow shades, making them favorites for covering a garden wall or fence. Blooms attract both butterflies and hummingbirds and release a sweet, heady aroma. Plants that grow taller than 36 inches are typically referred to as climbing or vining sweet peas. This group is divided into three categories. In areas that do not experience a hard winter freeze, sweet peas can be planted in either spring or fall.
Old Fashion or Heirloom
Traditional sweet peas typically produce less showy blooms than newer cultivars, but they more than make up for this with their fragrance. These are likely the flowers your grandmother grew in her flowerbed -- before plant breeders began their work to improve flower color and size at the expense of fragrance. Many old-fashioned varieties emit orange-honey scents that hang in the air. Those who wish to cultivate the feel of days gone by often choose antique or heirloom sweet peas for their delightful fragrance and pastel blooms.
Early Flowering
Early sweet peas perform best in areas where winters are mild, because they can be planted in late summer to produce winter blooms. Planting sweet peas at the same time you plant your fall bulbs produces early spring blooms. But that doesn't mean they are limited to warm climates. These early-flowering sweet peas can be planted in late spring in cold climates for summer blooms. These sweet peas typically require 10 hours of daylight to induce blooming.
Summer Flowering
These sweet peas require 12 to 15 hours of daylight to bloom and are typically planted in the spring. Although not as fragrant as the old-fashioned or heirloom varieties, some cultivars produce good fragrance. Flowers are larger and showier than either early-blooming or heirloom sweet peas and run the gamut of color, including blue. If you are looking for showy blooms, summer-flowering sweet peas may be the answer.
Each category contains a wide selection of cultivars that vary in both bloom size and fragrance. All prefer full sun to partial shade and like evenly moist, well-drained soil. These climbers require support to keep them upright but are not particular to what they climb. Trellises in many shapes and forms are readily available, but these hardy flowers will cover a fence or garden wall, as long as they have something to wind around. Sweet peas do not produce tendrils but instead twine the main vines around an upright structure as the reach for the sky, often achieving heights of 10 feet or more, depending on the cultivar.
Photo Credits
- Jupiterimages/Photos.com/Getty Images


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Home > Services > Health - Fitness Program
The Green World USA is a transnational group of companies engaging itself in scientific research and development' manufacturing of pharmaceuticals' herbal medicine and herbal cosmetics of a complete systems-oriented nature with its headquarters based in Michigan of the United States of America and manufacturing facilities in South California. It is registered and accredited by FDA (Food and Drug Administration) of USA. Then Dr. Deming Li' the founder of the Green World was determined to take the herbal systems especially the traditional Chinese herbal systems and the outcome of her scientific research and to introduce these wonderful herbal treasures to everyone' everywhere in the world.
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Welcome to the National Honor Society for Dance Arts
The National Honor Society for Dance Arts™ (NHSDA) is a program of the National Dance Education Organization, created to recognize outstanding artistic merit, leadership, and academic achievement in students studying dance in public and private schools in K-12 education, dance studios, cultural/community centers, performing arts organizations, and post-secondary education. The NHSDA offers three levels of induction: The Junior Program for grades 6-8, The Secondary Program for grades 9-12, and The Collegiate Program for students in college or university dance programs. Establishing an NHSDA chapter in your school is one very important way that you can honor your students and help advance the field of dance education.
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ThomasRed is a prominent player from Belgium. He was the 4th and 6th President of the United Netherlands, and was the President who had the longest non-consequtive termn in the history of the (United) Netherlands.
In June he became Party president of Belgian Party and on July 6 he became the first Belgian president of the United Netherlands when he won the elections from Frerk. In August he also won the elections. In August 2009 he wasn't very active due to RL, but he managed to win on Presidential elections again in September 2009. In October, he got successed by ArtemIvanov, an independant candidate who ran in name of the Libertarian Social Democrats.
He was elected in Congress of the United Netherlands three times: May 2009, June 2009 and October 2009.
In 2014 he joined EclypsiaBE.
He was a member of Belgian Civilian Army for a long period of time. Afterwards he joined Belgian National Army where he ultimately become Commander and hold that position til now.
He is the Press director of newspaper Behind the Atomium.
Other Achievements
Name | Date | Location | Type |
1st BoDi National Belgian Honor | Day 1186 | Belgium | Won |
1st BoDi Life Achievement Award | Day 1186 | Belgium | Nominated |
2nd BoDi National Belgian Honor | Day 1257 | Belgium | Nominated |
2nd BoDi Life Achievement Award | Day 1257 | Belgium | Won |
2nd BoDi Award of Merit / Honorary Belgian | Day 1257 | Belgium | Won |
1st BoDi Award for the Silent Worker | Day 1397 | Belgium | Nominated |
4th BoDi Life Achievement Award | Day 1726 | Belgium | Won |


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The Nobel Prize in Physics 1974 Martin Ryle, Antony Hewish
Links to other sites
Antony Hewish' page at Cambridge University
Interview with Professor Antony Hewish from BBC Archive: Radio telescopes, pulsars and why stars 'twinkle'
Your mission is to arrange an amazing laser party!
Read more about the Nobel Prize in Physics.
All you want to know about the Nobel Prize in Physics!
Copyright © Nobel Media AB 2017


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Survival games for camping bring together education and fun to both teach and allow participants to practice skills vital for enduring harsh conditions in the outdoors. Three of the most basic needs for survival are food, water and shelter. Fire supplements each of these and offers other benefits that can help your chances for rescue, health and well-being in life-or-death situations. The Girl Scouts and Boy Scouts of America serve as a valuable resource for survival techniques.
Other People Are Reading
Practice locating sources of food by identifying tracks and edible plants of the area. A nature hike offers opportunities for teams to point out each sign of animal tracks, scat or presence and each patch of wild food. Teams can compete for points, with leaders assigning bonus points if participants can offer additional information, such as how to harvest and prepare the food, what season or under what conditions it is edible, and identifying the useful parts of the plant. To follow up, challenge the participants to gather materials and cook a meal from wild ingredients.
To challenge participants with collecting water, leaders may start with a demonstration of rudimentary methods for gathering water and a discussion of the principles involved. Follow up by holding a contest to see who can collect the most water within a given period of time. All participants can use the same method, or each can choose a different one from the demonstration. Additionally, participants can start from camp and fan out across the area with markers to discover locations where the most acceptable water sources can be found. Leaders may set a time or distance limit, then discuss features of each find and award points based on their practicality.
Compete to see who can build the best emergency shelter out of supplies gathered from the area. Conditions for the game varies depending upon the location, materials available and the weather, so the game offers many permutations. Leaders can place subjective limitations, such as only allowing downed wood or dead materials, or setting a time for completion. For a tougher contest, participants could be required to live in their shelter for day. Rewards for winners of the games may include luxury food items or freedom from camp chores for one meal or one day.
Fire serves multiple purposes for those in survival situations, and each of these can inspire a survival game. The games may range from who can build the largest or smokiest signal fire to who can achieve the tallest flame or build a fire that can burn through a string at a set height before all others. One of the biggest challenges, and one that forces participants to consider scarcity and value of their resources, is to build a fire with one match. This requires foresight, planning and proper construction. Fire safety procedures and proper methods to extinguish a fire make a good accompaniment to the game. The fires can then be used to cook food and purify water or to heat a shelter, making combinations of skills tests for a multi-stage survival game possible.


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Tracing Graph Paper - 10/12 CountYarn Tree $6.00 $5.39 + $3.39 shipping within the U.S. (1 review) In Stock. Usually ships in 1-2 business days. Item: 123-2522 - Type: Needlecraft Accessories. Pad of 18 sheets--9 sheets of 10 count paper and 9 sheets of 12 count paper. Printed on special paper that is strong, yet still transparent enough to trace through. Graph Paper makes it easy to make your cross stitch designs. School logos, special occasion mementos, your home, or special sayings are just a few ideas for charting your own! Use the tracing graph paper to make a graph from a photo or sketch. Just trace the different colors; then go back and put in the color codes! All of our graph paper is printed with black ink so you can easily make copies of your finished design. 8 1/2"x11" pads. 123Stitch.com ships via the U.S. Postal Service to anywhere in the world. Orders to the U.S. typically arrive 3-5 days after we ship. Shipping charges are shown in your shopping cart every time you add an item. If you are not completely satisfied with any of our products, you can return it to us within 30 days for a full refund. |
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on 04/12/2016 - Great Product
I used this product to turn a picture my granddaughter drew into a cross stitch framed memory. Love it.
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The New Prague High School Class of 1950 will hold its 55-year reunion on Saturday, July 9, at the Park Ballroom Clubroom in New Prague.The group will attend 4 p.m. Mass at St. Wenceslaus Church, followed a 5 p.m. social hour and dinner at the clubroom.
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SC10 4X4 Aluminum Rear Hub Set (Blue) by Exotek Racing
Exotek Racing
Check Store Availability
Please be aware that pricing and product availability in-store may vary from what's available online because each store is independently owned and operated.
This is an optional Exotek SC10 4X4 Aluminum Rear Hub Set. These precision machined aluminum rear hubs for the SC10 4x4 feature vertical ball stud mounting for quick and precise roll center changes. Simply use 3mm shims to raise or lower the ball stud height. Precision machined from T6 alloy for precise fit, lightweight design and heavy duty use. Uses the stock bearings and maintains the stock toe geometry.
This product was added to our catalog on April 15, 2015.


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Internet Telephony Magazine Click here to read latest issue Subscribe for FREE - Click Here
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IoT EVOLUTION MAGAZINE Click here to read latest issue Subscribe for FREE - Click Here
There are few things more purely distressing in life than the sudden loss of power, especially when working. Lost data, lost productivity, and lost time all add up to a situation most would rather not be in. Recently, Delta--who ranks as the world's leader in both DC brushless fans and switching power supplies--announced the introduction of a new line of Amplon M Series UPS--Uninterruptible Power Supply--systems throughout Russia and Europe.
The new line of the Amplon M Series offers a variety of power ratings--starting at 1 kVA and then proceeding to 1.5 kVA, 2 kVA and ultimately 3 kVA--and is specifically geared toward providing multiple-application backup power thanks to its line-interactive status and sine-wave quality generation. Not only is the Amplon M Series lineup geared to be ultimately flexible--suggested uses include as a backup for servers, VoIP applications, and networking processes--it's also designed to provide that at a comparatively low total investment. Image via www.opensource.com
Since the Amplon M Series is line-interactive, it boasts an AVR--Automatic Voltage Regulator--system that keeps the power flowing steadily out from the device and ensuring the least chance possible that power surges or the like will interfere with availability. That's especially important when using the Amplon M Series in applications like VoIP or server and networking matters, because reliability and up-time reach paramount importance.
The general manager of Delta MCIS, Dr. Charles Tsai, explained the benefits of the Amplon M Series with his remarks: "The Amplon M is an absolutely reliable power protection solution for small and medium businesses that will provide the backbone for business operations continuity. We are proud to launch this new product with its high availability, flexibility and excellent power performance. Delta MCIS continues to engineer new UPS development to provide a full range of UPS offerings that fulfill a wide range of customer requirements."
Indeed, having protection against sudden power outages is important. Not just for the obvious reason of giving users a chance to save their work before a power outage completely removes their ability to operate, but because it also allows the larger systems picture--the servers, the network, the VoIP systems, and all those other systems that keep the individual users able to operate and be productive in the first place--to continue operating through that very same power outage.
They're temporary solutions to a problem that often has indefinite origins and duration, but UPS systems may well mean the difference between hours of lost productivity and an early end to the day.
ITEXPO: February 14 - 16, 2018 | Fort Lauderdale, Florida
Real Time Web Solutions Conference: February 14 - 16, 2018 | Fort Lauderdale, Florida
IoT Evolution Expo : January 22-25, 2018| Orlando, Florida


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Happiness and good wishes multiply when sent with a gift like this hamper from the house of Ferns n Petals. This Eid send your good wishes along with this hamper and make your dear ones feel special. Your gift contain: Ribbon, Brown Wooden Tray, Chocolate Date with Almond,
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Please refer to the expiration date on the package and consume your edibles before that.


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The Horse Forum
- Trail Riding
- - Best cinch for trail riding
Best cinch for trail riding what are you opinions on which cinch is the best for trail riding. I am moving my horses up to my house in the country soon and i want a good cinch for trail riding for hours and up and down hills. There will also be walking across creeks. What do you guys think would be the best cinch for this? thanks!!!!!:D |
Can never go wrong with a good ole mohair girth. Posted via Mobile Device |
47 Attachment(s) I like mohair or felt. Not a fan of neoprene at all. I have a felt smart cinch that I looovvvee. Never rubbed or anything and I often take my horses to the beach, through muddy creeks, and tough terrain. Posted via Mobile Device |
I second a good mohair string girth. That's all I use and I can't complain at all. I've got a couple of smart cinches (hate them with a passion) that I'm trying to wear out right now, but as soon as I'm through with them, I'm going to start buying these CLASSIC - Classic Roper Nylon Center Cinch - NRSworld.com |
Lonestar, just curious why you don't like neoprene? I have the airflex and love it, wife just switched her pony to a neoprene girth and loves it too... |
My biggest problem with the smart cinches is the fact that the bottom roller is so wide. Whenever I cinch up, as the latigo tightens, it often slides off-center so that the latigo holes are offset from the buckle tongue. It wouldn't be a big deal except it takes me a minute to re-adjust...and since I ride a lot of young horses who can sometimes be unpredictable the first time the cinch is done up, that minute can become a big deal in a hurry. Also, the ones I've got (though this feature isn't exclusive to the smart cinches) have the leather middle in them like this http://ts2.mm.bing.net/th?id=H.48726...41373&pid=15.1 Unfortunately, that leather gets wet...constantly. I have to double check it every morning after it has dried to make sure that the leather hasn't curled up around the edges. Made that mistake once and ended up with a gall the size of a quarter on a horse. Very not cool. So now, I'll only buy cinches with the nylon center in them. |
I'm a big fan of neoprene cinches. Posted via Mobile Device |
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<<<Looking for my Lupus Awareness Month giveaway?>>>
Welcome to the 3rd day of my week as the Growing In Unity gal!
I am so thankful for each of you that have stopped by so far to check out and comment on each of my projects using Unity Stamp Company stamps. Be sure to comment on all five of my Growing In Unity posts this week if you want a chance to win some free stamps!
If you are new here or aren't already a follower: I'd love to have you follow my blog!
If you are one of my regular readers: Thanks for coming back! I love having you!
If you are on Facebook, please "like" Ink Blots by TRD.
Today's card is a simple one to create, really. I think it looks more involved than it really is.
First, I placed some strips of washi tape on my card base. Then, I die cut a piece of cardstock (the same size as the front of the card) and saved both the positive and negative cuts. I adhered the negative die cut to the card front and then added the positive cuts as embellishments. It's all topped off with a bit of twine and one of my favorite new sentiments from Unity - this one is from the A Lovely Butterfly Day Itty Bitty.
(If you are interested in seeing a couple more cards created with the same basic supplies - see my post here.)
From the comments that are left on each of my posts this week I will be randomly choosing someone to win any two Itty Bittys (winner's choice!) from Unity Stamp Company - to be sent to a domestic address only, please.
Washi Tape: My Mind's Eye;
Stamps: Unity Stamp Company (A Lovely Butterfly Day);
Ink: Memento (London Fog);
Twine: The Twinery (Gold Shimmer);
Dies: Top Dog Dies (Pretty Petals Dies Set);
Tools: Fiskars (Trimmer, Scissors); Scor-Pal (Scoring Board);


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Melt In Your Mouth Honey Lemon Merry Christmas Cookies
"These are a Christmas family tradition at our house and they don't last long. Even better (more moist and very sweet) the second and third day if kept...
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Melt In Your Mouth Flaky Butter Brioches Recipe
Flaky French rolls with top knots-I always make these each year for Thanksgiving and Christmas...and any special celebration. But they are easy enough...
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Buttery Melt-In-Your-Mouth Sweet Cornbread
This is wonderful warm out of the oven with syrup
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Melt in Your Mouth Pralines
"They are terrific! Not chalky, but creamy and delicious. These can be stored at room temperature, or frozen. Can't beat pralines from Louisiana!"
salt, +baking soda, +butter, +buttermilk, +powdered sugar, +heavy cream, +pecan, +vanilla extract, +sugar
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Melt In Your Mouth Chicken Recipe
Tried many times. True every time. Awesome recipe I obtained from a church friend several years ago. This is a regular in my home and nobody ever tires...
salt, +black pepper, +chicken, +cream of chicken soup, +garlic powder, +stuffing, +monterey jack cheese, +sherry
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Melt in Your Mouth Banana Bread
This bread is so yummy that it will be gone before you know it.
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Melt-In-Your-Mouth Meat Loaf
"'When my husband and I were first married, he refused to eat meat loaf because he said it was bland and dry,' recalls Suzanne Codner of Starbuck, Minnesota....
salt, +ground beef, +brown sugar, +eggs, +ketchup, +milk, +mustard powder, +sage, +bread crumbs, +worcestershire sauce
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Melt In Your Mouth Blueberry Cake
"This is a nice tender cake - one of my Mom's specialties from years ago. It is a great cake to take along to a picnic."
salt, +baking powder, +blueberries, +butter, +egg whites, +egg yolks, +flour, +milk, +vanilla extract, +sugar
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Northern White Rhino: Now Ceratotherium cottoni? Using genetic data and re-assessing physical evidence, scientists write that they have uncovered a new species of rhino (Ceratotherium cottoni), long considered by biologists as merely a subspecies (Ceratotherium simum cottoni). Researchers write in an open access PLoS ONE paper published last year that evidence has shown the northern white rhino is in fact a distinct species from the more commonly known—and far more common—southern white rhino (Ceratotherium simum, formerly Ceratotherium simum simum). If the scientific community accepts the paper's argument it could impact northern white rhino conservation, as the species would overnight become the world's most endangered rhino species with likely less than ten surviving. The researchers found that the skull of the northern and the southern white rhino are 'readily distinguished' and that the animals can be differentiated simply by looking at them. In addition, the genetic study found that the northern and southern white rhino diverged around a million years ago. "Its taxonomic distinctiveness argues strongly for its conservation, as its demise will mean the permanent loss of a unique taxon that is irreplaceable," write the authors. Currently 8 northern white rhinos are confirmed to survive, however four of these though are no longer able to breed. The last four northern white rhinos capable of saving the species were transferred from Dvur Králové Zoo (Czech Republic) in 2009 to Ol Pejeta Conservancy in Laikipia/Kenya where they are guarded around the clock (the photo shows Suni, a male northern rhino, arriving in Africa). While dire, the situation may not be utterly hopeless. "The admirable success of the conservation histories of the Southern white rhino and the Indian rhino, both of which were brought back from the brink of extinction by successful conservation efforts, does, however, hold out hope that the northern white may yet be saved for posterity," write the authors. Conservationists hope that by providing the four rhinos—two males and two females—with their natural habitat will provide a better chance for breeding. Rhinos are notoriously difficult to breed in captivity. CITATION: Groves CP, Fernando P, Robovský J (2010), The Sixth Rhino: A Taxonomic Re-Assessment of the Critically Endangered Northern White Rhinoceros. PLoS ONE 5(4): e9703. Download complete article: PLoS ONE: The Sixth Rhino: A Taxonomic Re-Assessment of the Critically Endangered Northern White Rhinoceros
I'd hate to burst your bubble but 2 males and 2 females is not enough to resurrect a species. If we want to avoid inbreeding, we'll have to either crossbreed them with Southern white rhinos which can be plagued with problems or get as much genetic material from dead rhinos and clone them and as we all know from Jurassic Park, you could end up with a slew of unintended consequences.
tigris115; Please explain how a Hollywood Science Fiction Movie has any relevance to....... well anything!? Other than entertainment value. Ado Elephant Park was founded with only 11 elephants. I believe it is actually at a level now where population control is a challenge. Not saying this is the same scenario, but good common sense conservation combined with real science can do wonders. At this point, that rhino population and inbreeding is the least of their worries. I think multiplication of that species needs to be the priority, inbred or not.
Ok, the JP part was just a stupid joke. But I'm still think that 2 breeding pairs is cutting it a bit close. Don't get me wrong, we've had miracles in conservation (like Addo), I'm just wondering if these rhinos are too far gone to really do anything.


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We've got 3 definitions for NFIB »
What does NFIB stand for?
What does NFIB mean? This page is about the various possible meanings of the acronym, abbreviation, shorthand or slang term: NFIB.
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NFIB | National Federation of Independent Business | |
NFIB | National Federation of Independent Businesses | |
NFIB | National Foreign Intelligence Board Governmental » US Government -- and more... |
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"NFIB." Abbreviations.com. STANDS4 LLC, 2017. Web. 28 Mar. 2017. <http://www.abbreviations.com/NFIB>.


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Holiday Inn Express Louisville Northeast
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Holiday Inn Express Louisville Northeast
About the hotel
Property Location
With a stay at Holiday Inn Express Louisville Northeast in Louisville (Northeast Louisville), you'll be convenient to Springhurst Towne Center Shopping Center and E P Tom Sawyer State Park. This hotel is within close proximity of Chamberlain Pointe Shopping Center and Avish Gardens Shopping Center.
Make yourself at home in one of the 81 air-conditioned rooms featuring refrigerators and microwaves. Your room comes with a pillowtop bed. Wired and wireless Internet access is complimentary, while 32-inch flat-screen televisions with cable programming provide entertainment. Private bathrooms with shower/tub combinations feature complimentary toiletries and hair dryers.
Be sure to enjoy recreational amenities including an indoor pool and a fitness center. Additional features at this hotel include complimentary wireless Internet access and a fireplace in the lobby.
A complimentary buffet breakfast is included.
Business, Other Amenities
Featured amenities include complimentary high-speed (wired) Internet access, a business center, and express check-in. Planning an event in Louisville? This hotel has facilities measuring 480 square feet (43 square meters), including a meeting room. Free self parking is available onsite.
Hotel Facilities
- Safe-deposit box at front desk
- Number of floors - 3
- Total number of rooms - 81
- Dry cleaning/laundry service
- Number of buildings/towers - 1
- Smoke-free property
- Luggage storage
- Express check-in
- Free self parking
- Business center
- One meeting room
- Laundry facilities
- Conference space size (feet) - 480
- Free WiFi
- Fitness facilities
- Elevator/lift
- Express check-out
- Fireplace in lobby
- Free wired high-speed Internet
- Multilingual staff
- Indoor pool
- RV, bus, truck parking
- Free breakfast
- Accessible bathroom
- Computer station
- Free newspapers in lobby
- Roll-in shower
- Conference space size (meters) - 43
- In-room accessibility
- 24-hour front desk
Room Facilities
- Free toiletries
- Phone
- Free WiFi
- Free wired high-speed Internet
- Private bathroom
- Free cribs/infant beds
- Cable TV service
- In-room climate control (air conditioning)
- Desk
- Connecting/adjoining rooms available
- Daily housekeeping
- Refrigerator
- Premium TV channels
- Flat-panel TV
- Shower/tub combination
- Pay movies
- Microwave
- Air conditioning
- Hair dryer
- Free local calls
- Iron/ironing board
- Blackout drapes/curtains
- Free weekday newspaper
- Pillowtop mattress
- Coffee/tea maker
Hotel Policy
Know Before You Go
- Up to 3 children 18 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.
- Only registered guests are allowed in the guestrooms.
- The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
- Alcohol is not served at this property.


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Specific Issues Reports for H.CON.RES.34 by Northeast Utilities, 112th Congress
Content of Specific Issue field
FY 2012 Labor, HHS Appropriations; All provisions related to Low Income Home Energy Assistance Program (LIHEAP) funding. H.R. 1473, Department of Defense and Full-Year Continuing Appropriations Act, 2011; All provisions related to LIHEAP. H. Con. Res. 34, Budget of the United States Government, FY 2012; All provisions related to LIHEAP funding, Energy Effeciency and Renewable Energy budget, and electricity budget.
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Dawn Henderson
116 posts and 1 follower since Nov 2011
Angry Birds cake for friends son. Buttercream with Fondant birds
Tags: angry birds birthday black gold yellow blue brown green red orange white cake banana
Apr 2012
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Michal Bulla ... Apr 2012
Dawn Henderson ... Apr 2012
Thanks so much!
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text: Meta Battle Subway PokeBase - Pokemon Q&A Login Register All Activity Questions Unanswered Tags Users Ask a Question Rules Chat Ampharos or charizard? 0 votes I have the mega ring for bolth xy charizard ampharos asked Nov 19, 2013 by N the Champion Don't you mean mega stones? What's your team? Please log in or register to add a comment. Please log in or register to answer this question. 1 Answer 1 vote Best answer It depends, charizard x and ampharos both have dragon type, if you need a good sweeper, choose charizard. But ampharos is good due to good stats and a nice move set, it depends on what you want. Want a fire type? Choose charizard. Electric? Ampharos. Depends on what you want. But are you using charizardite x or y? answered Nov 19, 2013 by zachydoodle selected Nov 19, 2013 by N the Champion Y I'm using y OK, you should probably use charizard, because mega charizard has high stats and packs a real punch Please log in or register to add a comment. Related questions Luxray or Ampharos?In the X and Y game? Ampharos or Jolteon? Mega Ampharos or Mega Manectric? If I have a Ampharos and a Manectric with Minus, would the ability work? Why Can't Ampharos learn Dazzling Gleam? How would I obtain an Ampharos in X? Thunder or Thunder Punch on Ampharos? Ampharos or Jolteon in HeartGold? Useful Meta Posts Vote for Sun/Moon moveset questions How to change your username How to add a user avatar Why was my post hidden? The points system Question etiquette Flags vs Downvotes Anime FAQ


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Transcription of viral DNAs. Lecture 14 Flint et al. pp. 253 – 277.
General points For RNA viruses, most viral mRNAs are synthesized by Viral RDRP For DNA viruses, viral mRNAs are generally synthesized by cellular RNA polymerases. –Some exceptions: e.g. Poxviruses have their own DNA-dependent RNA polymerase. Transcription and expression of viral genes occurs in a strictly defined, reproducible sequence. Generally: –Genes for viral enzymes and regulatory proteins are transcribed early in infection. –Genes for structural proteins are transcribed later.
The relative simplicity of viral systems has enabled their use in elucidating the general properties of cellular RNA transcription What we know about transcriptional control is based on virology. Therefore, we must first digress back to cellular transcription.
Cellular transcription DNA dependent RNA polymerase Cells contain 3 DNA dependent RNA polymerases (see Table 8.1) RNA pol I: transcribes pre-rRNA; no known viral templates RNA pol II: transcribes pre-mRNA & snRNA: polymerase for most viral DNAs. RNA pol III: transcribes pre-tRNAs, 5S rRNA, U6 snRNA; polymerase for some viral DNAs.
Finding the right place to start The transcriptional machinery must: –Be directed to initiate transcription at the correct location on a DNA template (the transcriptional start site). –elongate through the entire gene –Be directed to terminate transcription at the correct location. All of these functions require the assistance of –Cis-acting sequences along the DNA –Trans-acting factors (accessory proteins)
Nuclear localization DNA dependent RNA polymerases located in nucleus Viruses that usurp these functions must localize their templates for this machinery into the nucleus. Three variations on this theme (Table 8.2) 1.Viral genome looks like a chromosome: -Associated with host-cell nucleosomes or viral proteins that look like nucleosomes. e.g. Papilloma- and Polyomaviruses 2.Viral ssDNA converted to dsDNA in viral particle. dsDNA then imported into nucleus. e.g. hepadnaviruses 3.Viral RNA reverse transcribed in viral particle. cDNA imported into nucleus and integrated into genome. e.g. Retroviruses.
Transcription by RNA Pol. II. At least 40 proteins required: Pol. II itself + accessory proteins. Accurate transcription initiated at the promoter. Promoter + additional DNA sequence that controls transcription = Transcriptional control region (TCR). –The adenovirus type 2 major late promoter was the first TCR ever recapitulated in vitro. Initiation is a multistep process: –Promoter recognition by RNA Pol. II –Formation of open initiation complex (unwinding) –Promoter clearance –3' movement of complex away from promoter
Transcription by RNA Pol. II. Fig 8.2
Promoters (Fig. 8.1) Core promoters Contain all the information necessary for recognition of initiation start site. Direct RNA Pol. II complex to begin transcription. Contain TA-rich "TATA box" sequences. –Thermodynamically easy to unwind. –Located 20 – 35 bp upstream (5') of start site. Contain Initiators Sort sequences Specify accurate but inefficient transcription
Promoters (Fig. 8.1)
Regulation of Pol. II transcription Transcription must be regulated: genes must be turned on and off in temporal patterns Viral gene expression: early and late genes Transcriptional regulation is controlled by: –Cis-acting sequences in DNA – both local and distal –Trans-acting factors – both protein and RNA Trans-acting factors specifically bind to cis- acting sequences to either –activators stimulate transcription –repressors prevent transcription
An enormous number of sequence- specific transcriptional regulators. Basic properties (Fig. 8.7) Modular organization – built from discrete structural and functional domains. DNA binding module – targets protein to a specific DNA sequence Activation (or repression) domain – interacts directly or indirectly with the RNA polymerase. Dimerization domain – activation tends to require interacts with other transacting factors. Provides a way to fine tune activity. –Homo-dimer: interaction of two copies of the same factor –Hetero-dimer: interaction of two different factor.
Modular organization of sequence-specific transcriptional regulators
Example: Regulation of HIV-1 transcription by host and virus encoded factors. The HIV-1 transcriptional control region: Located in the 5' region of the proviral genome. Divided into 3 enhancer regions. –Promoter: Contains binding sites for TFIId and SP I factors: recruit RNA Pol II to promoter. –Core enhancer: binding sites for Nf-kB and Ets-1. These proteins are only active in growing T-cells, i.e. those exposed to antigen. –Upstream enhancer region: Binding sites for Ets-1, Gata-3, Lef, Nf- IL6. These proteins are only active in hematopoietic cells: helps to narrow the specificity of transcription.
Transcriptional control by viral trans- acting factors: Tat and Tar (Fig. 8.13). By itself, RNA Pol II does not elongate well away from the transcriptional control region: this provides a way to downregulate (negatively control) HIV replication. This is called poor processivity. To overcome this, the virus produces two trans- acting factors: –Tar: an RNA trans-acting factor –Tat: a protein trans-acting factor These two factors work synergistically to recruit other host proteins that stimulate and enhance the processivity of the elongating RNA Pol II complex.
Transcriptional control by viral trans-acting factors: Tat and Tar (Fig. 8.13).
DNA virus transcriptional programs Viral proteins act to either enhance or repress transcription of viral genes in an orderly manner.
DNA virus transcriptional programs SV40: Genome contains 2 transcriptional units: Early and Late. Early unit encodes the Large T antigen. Large T antigen acts to enhance transcription of the late unit, leading to virus production.
DNA virus transcriptional programs Adenovirus type 2: Has 3 transcriptional units: Immediate early, early, and late. E1A protein produced by immediate early unit E1A activates early unit, represses late unit. E2 protein encoded in early unit. E2 activates transcription of the late unit.
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