text: DSpace Community: http://hdl.handle.net/2445/7209 2018-12-12T02:28:51Z Nutrients, control of gene expression and metabolic homeostasis http://hdl.handle.net/2445/126685 Title: Nutrients, control of gene expression and metabolic homeostasis Author: Haro Bautista, Diego 2018-12-04T08:23:28Z A tryptophanol-derived oxazolopiperidone lactam is cytotoxic against tumors via inhibition of p53 interaction with murine double minute proteins http://hdl.handle.net/2445/126421 Title: A tryptophanol-derived oxazolopiperidone lactam is cytotoxic against tumors via inhibition of p53 interaction with murine double minute proteins Author: Soaresa, Joana; Raimundo, Liliana; Pereira, Nuno A. L.; dos Santos, Daniel J. V. A.; Pérez Bosch, Maria; Queiroz, Glória; Leão, Mariana; Santos, Maria M. M.; Saraiva, Lucília Abstract: Inactivation of the p53 tumor suppressor protein by interaction with murine double minute (MDM) proteins, MDM2 and MDMX, is a common event in human tumors expressing wild-type p53. In these tumors, the simultaneous inhibition of these interactions with MDMs, for a full p53 reactivation, represents a promising anticancer strategy. Herein, we report the identification of a dual inhibitor of the p53 interaction with MDM2 and MDMX, the (S)-tryptophanol derivative OXAZ-1, from the screening of a small library of enantiopure tryptophanol-derived oxazolopiperidone lactams, using a yeast-based assay. With human colon adenocarcinoma HCT116 cell lines expressing wild-type p53 (HCT116 p53+/+) and its p53-null isogenic derivative (HCT116 p53−/−), it was shown that OXAZ-1 induced a p53-dependent tumor growth-inhibitory effect. In fact, OXAZ-1 induced p53 stabilization, up-regulated p53 transcription targets, such as MDM2, MDMX, p21, Puma and Bax, and led to PARP cleavage, in p53+/+, but not in p53−/−, HCT116 cells. In addition, similar tumor cytotoxic effects were observed for OXAZ-1 against MDMXoverexpressing breast adenocarcinoma MCF-7 tumor cells, commonly described as highly resistant to MDM2-only inhibitors. In HCT116 p53+/+ cells, the disruption of the p53 interaction with MDMs by OXAZ- 1 was further confirmed by co-immunoprecipitation. It was also shown that OXAZ-1 potently triggered a p53-dependent mitochondria-mediated apoptosis, characterized by reactive oxygen species generation, mitochondrial membrane potential dissipation, Bax translocation to mitochondria, and cytochrome c release, and exhibited a p53-dependent synergistic effect with conventional chemotherapeutic drugs. Collectively, in this work, a novel selective activator of the p53 pathway is reported with promising antitumor properties to be explored either alone or combined with conventional chemotherapeutic drugs. Moreover, OXAZ-1 may represent a promising starting scaffold to search for new dual inhibitors of the p53 MDMs interaction. 2018-11-23T16:00:02Z Comparison of 24-h volume and creatinine-corrected total urinary polyphenol as a biomarker of total dietary polyphenols in the InCHIANTI Study http://hdl.handle.net/2445/126148 Title: Comparison of 24-h volume and creatinine-corrected total urinary polyphenol as a biomarker of total dietary polyphenols in the InCHIANTI Study Author: Zamora-Ros, Raul; Rabassa Bonet, Montserrat; Cherubini, Antonio; Urpí Sardà, Mireia; Llorach, Rafael; Bandinelli, Stefania; Ferrucci, Luigi; Andrés Lacueva, Ma. Cristina Abstract: Polyphenols have beneficial effects on several chronic diseases but assessing polyphenols intake from self-reported dietary questionnaires tends to be inaccurate and not very reliable. A promising alternative is to use urinary excretion of polyphenols as a proxy measure of intake. The best method to assess urinary excretion is to collect 24-h urine. However, since collecting 24-h urine method is expensive, time consuming and may be difficult to implement in large population-based studies, measures obtained from spot urine normalized by creatinine are commonly used. The purpose of the study was to evaluate the correlation between polyphenols dietary intake and total urinary polyphenol excretion (TPE), expressed by both 24-h volume and urinary creatinine normalization in 928 participants from the InCHIANTI study. Dietary intake data were collected using a validated food frequency questionnaire. Urinary TPE was analyzed by Folin-Ciocalteau assay. Both urinary TPE expression models were statistically correlated (r=0.580), and the partial correlation coefficient improved (pr=0.722) after adjusting for the variables that modify the urinary creatinine excretion (i.e. gender, age, BMI, physical activity and renal function). In crude models, polyphenol intake was associated with TPE corrected by 24-h volume (r=0.211; P<0.001), but not with creatinine normalization (r=0.014; P=0.692). However, urinary TPE expressed by creatinine correction was significantly correlated with dietary polyphenols after adjusting for covariates (pr=0.113; P=0.002). We conclude that urinary TPE expressed by 24-h volume is a better biomarker of polyphenol dietary intake than by urinary creatinine normalization. After covariate adjustment, both can be used for studying the relationships between polyphenol intake and health in large-scale epidemiological studies. 2018-11-15T15:32:40Z Polyphenol fraction of extra virgin olive oil protects against endothelial dysfunction induced by high glucose and free fatty acids through modulation of nitric oxide and endothelin-1 http://hdl.handle.net/2445/125966 Title: Polyphenol fraction of extra virgin olive oil protects against endothelial dysfunction induced by high glucose and free fatty acids through modulation of nitric oxide and endothelin-1 Author: Storniolo, Carolina Emilia; Rosselló Catafau, Joan; Pintó Sala, Xavier; Mitjavila Cors, Maria Teresa; Moreno Aznárez, Juan José Abstract: Epidemiological and clinical studies have reported that olive oil reduces the incidence of cardiovascular disease. However, the mechanisms involved in this beneficial effect have not been delineated. The endothelium plays an important role in blood pressure regulation through the release of potent vasodilator and vasoconstrictor agents such as nitric oxide (NO) and endothelin-1 (ET-1), respectively, events that are disrupted in type 2 diabetes. Extra virgin olive oil contains polyphenols, compounds that exert a biological action on endothelial function. This study analyzes the effects of olive oil polyphenols on endothelial dysfunction using an in vitro model that simulates the conditions of type 2 diabetes. Our findings show that high glucose and linoleic and oleic acids decrease endothelial NO synthase phosphorylation, and consequently intracellular NO levels, and increase ET-1 synthesis by ECV304 cells. These effects may be related to the stimulation of reactive oxygen species production in these experimental conditions. Hydroxytyrosol and the polyphenol extract from extra virgin olive oil partially reversed the above events. Moreover, we observed that high glucose and free fatty acids reduced NO and increased ET-1 levels induced by acetylcholine through the modulation of intracellular calcium concentrations and endothelial NO synthase phosphorylation, events also reverted by hydroxytyrosol and polyphenol extract. Thus, our results suggest a protective effect of olive oil polyphenols on endothelial dysfunction induced by hyperglycemia and free fatty acids. 2018-11-09T14:53:06Z Dietary vitamin D intake and risk of type 2 diabetes in the European Prospective Investigation into Cancer and Nutrition: the EPIC-InterAct study http://hdl.handle.net/2445/125948 Title: Dietary vitamin D intake and risk of type 2 diabetes in the European Prospective Investigation into Cancer and Nutrition: the EPIC-InterAct study Author: Abbas, Sascha; Linseisen, Jakob; Rohrmann, Sabine; Beulens, Joline W. J.; Buijsse, Brian; Amiano, Pilar; Ardanaz, Eva; Balkau, Beverley; Boeing, Heiner; Clavel-Chapelon, Françoise; Fagherazzi, Guy; Franks, Paul W.; Gavrila, Diana; Grioni, Sara; Kaaks, Rudolf; Key, Timothy J.; Khaw, Kay Tee; Kühn, Tilman; Mattiello, Amalia; Molina Montes, Esther; Nilsson, Peter M.; Overvad, Kim; Quirós, J. Ramón; Rolandsson, Olov; Sacerdote, Carlotta; Saieva, Calogero; Slimani, Nadia; Sluijs, Ivonne; Spijkerman, Annemieke M. W.; Tjønneland, Anne; Tumino, Rosario; A., Daphne L. van der; Zamora-Ros, Raul; Sharp, Stephen J.; Langenberg, Claudia; Forouhi, Nita G.; Riboli, Elio; Wareham, Nicholas J. Abstract: BACKGROUND/OBJECTIVES: Prospective cohort studies have indicated that serum vitamin D levels are inversely related to risk of type 2 diabetes. However, such studies cannot determine the source of vitamin D. Therefore, we examined the association of dietary vitamin D intake with incident type 2 diabetes within the European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct study in a heterogeneous European population including eight countries with large geographical variation. SUBJECTS/METHODS: Using a case-cohort design, 11 245 incident cases of type 2 diabetes and a representative subcohort (N = 15 798) were included in the analyses. Hazard ratios (HR) and 95% confidence intervals (CIs) for type 2 diabetes were calculated using a Prentice-weighted Cox regression adjusted for potential confounders. Twenty-four-hour diet-recall data from a subsample (N = 2347) were used to calibrate habitual intake data derived from dietary questionnaires. RESULTS: Median follow-up time was 10.8 years. Dietary vitamin D intake was not significantly associated with the risk of type 2 diabetes. HR and 95% CIs for the highest compared to the lowest quintile of uncalibrated vitamin D intake was 1.09 (0.97-1.22) (P-trend = 0.17). No associations were observed in a sex-specific analysis. The overall pooled effect (HR (95% CI)) using the continuous calibrated variable was 1.00 (0.97-1.03) per increase of 1 mg/day dietary vitamin D. CONCLUSIONS: This observational study does not support an association between higher dietary vitamin D intake and type 2 diabetes incidence. This result has to be interpreted in light of the limited contribution of dietary vitamin D on the overall vitamin D status of a person. 2018-11-09T11:51:15Z Empirically-derived food patterns and the risk of total mortality and cardiovascular events in the PREDIMED study http://hdl.handle.net/2445/125272 Title: Empirically-derived food patterns and the risk of total mortality and cardiovascular events in the PREDIMED study Author: Martínez-González, Miguel Ángel, 1957-; Zazpe, Itziar; Razquin, C.; Sánchez-Tainta, A.; Corella Piquer, Dolores; Salas Salvadó, Jordi; Toledo, Estefanía; Ros Rahola, Emilio; Muñoz, M.A.; Recondo, J.; Gómez-Gracia, E.; Fiol Sala, Miguel; Lapetra, José; Buil-Cosiales, Pilar; Serra Majem, Lluís; Pintó Sala, Xavier; Schröder, Helmut; Tur, Josep A.; Sorlí, José V.; Lamuela Raventós, Rosa Ma.; Estruch Riba, Ramon Abstract: BACKGROUND & AIMS: There is little evidence on post hoc-derived dietary patterns (DP) and all-cause mortality in Southern-European populations. Furthermore, the potential effect modification of a DP by a nutritional intervention has not been sufficiently assessed. We assessed the association between a posteriori defined baseline major DP and total mortality or cardiovascular events within each of the three arms of a large primary prevention trial (PREDIMED) where participants were randomized to two active interventions with Mediterranean-type diets or to a control group (allocated to a low-fat diet). DESIGN: We followed-up 7216 participants for a median of 4.3 years. A validated 137-item food-frequency questionnaire was administered. Baseline DP were ascertained through factor analysis based on 34 predefined groups. Cox regression models were used to estimate multivariable-adjusted hazard ratios (HR) for cardiovascular disease (CVD) or mortality across quartiles of DP within each of the three arms of the trial. RESULTS: We identified two major baseline DP: the first DP was rich in red and processed meats, alcohol, refined grains and whole dairy products and was labeled Western dietary pattern (WDP). The second DP corresponded to a 'Mediterranean-type' dietary pattern (MDP). During follow-up, 328 participants died. After controlling for potential confounders, higher baseline adherence to the MDP was associated with lower risk of CVD (adjusted HR for fourth vs. first quartile: 0.52; 95% CI (Confidence Interval): 0.36, 0.74; p-trend <0.001) and all-cause mortality (adjusted HR: 0.53; 95% CI: 0.38, 0.75; p-trend <0.001), regardless of the allocated arm of the trial. An increasing mortality rate was found across increasing quartiles of the WDP in the control group (allocated to a low-fat diet), though the linear trend was not statistically significant (p = 0.098). CONCLUSIONS: Higher adherence to an empirically-derived MDP at baseline was associated with a reduced risk of CVD and mortality in the PREDIMED trial regardless of the allocated arm. The WDP was not associated with higher risk of mortality or cardiovascular events. 2018-10-10T14:48:49Z Statistical and biological gene-lifestyle interactions of MC4R and FTO with diet and physical activity on obesity: new effects on alcohol consumption http://hdl.handle.net/2445/125062 Title: Statistical and biological gene-lifestyle interactions of MC4R and FTO with diet and physical activity on obesity: new effects on alcohol consumption Author: Corella Piquer, Dolores; Ortega-Azorín, Carolina; Sorlí, Jose V.; Covas Planells, María Isabel; Carrasco, Paula; Salas Salvadó, Jordi; Martínez-González, Miguel Ángel, 1957-; Arós, Fernando; Lapetra, José; Serra Majem, Lluís; Lamuela Raventós, Rosa Ma.; Gómez-Gracia, Enrique; Fiol Sala, Miguel; Pintó Sala, Xavier; Ros Rahola, Emilio; Martí, Amelia; Coltell, Oscar; Ordovás, José M.; Estruch Riba, Ramon Abstract: Background Fat mass and obesity (FTO) and melanocortin-4 receptor (MC4R) and are relevant genes associated with obesity. This could be through food intake, but results are contradictory. Modulation by diet or other lifestyle factors is also not well understood. Objective To investigate whether MC4R and FTO associations with body-weight are modulated by diet and physical activity (PA), and to study their association with alcohol and food intake. Methods Adherence to Mediterranean diet (AdMedDiet) and physical activity (PA) were assessed by validated questionnaires in 7,052 high cardiovascular risk subjects. MC4R rs17782313 and FTO rs9939609 were determined. Independent and joint associations (aggregate genetic score) as well as statistical and biological gene-lifestyle interactions were analyzed. Results FTO rs9939609 was associated with higher body mass index (BMI), waist circumference (WC) and obesity (P<0.05 for all). A similar, but not significant trend was found for MC4R rs17782313. Their additive effects (aggregate score) were significant and we observed a 7% per-allele increase of being obese (OR = 1.07; 95%CI 1.01-1.13). We found relevant statistical interactions (P<0.05) with PA. So, in active individuals, the associations with higher BMI, WC or obesity were not detected. A biological (non-statistical) interaction between AdMedDiet and rs9939609 and the aggregate score was found. Greater AdMedDiet in individuals carrying 4 or 3-risk alleles counterbalanced their genetic predisposition, exhibiting similar BMI (P = 0.502) than individuals with no risk alleles and lower AdMedDiet. They also had lower BMI (P = 0.021) than their counterparts with low AdMedDiet. We did not find any consistent association with energy or macronutrients, but found a novel association between these polymorphisms and lower alcohol consumption in variant-allele carriers (B+/−SE: −0.57+/−0.16 g/d per-score-allele; P = 0.001). Conclusion Statistical and biological interactions with PA and diet modulate the effects of FTO and MC4R polymorphisms on obesity. The novel association with alcohol consumption seems independent of their effects on BMI. 2018-10-04T14:16:47Z Structural model of the hUbA1-UbcH10 quaternary complex: In silico and experimental analysis of the protein-protein interactions between E1, E2 and ubiquitin http://hdl.handle.net/2445/123857 Title: Structural model of the hUbA1-UbcH10 quaternary complex: In silico and experimental analysis of the protein-protein interactions between E1, E2 and ubiquitin Author: Correale, Stefania; de Paola, Ivan; Morgillo, Carmine Marco; Federico, Antonella; Zaccaro, Laura; Pallante, Pierlorenzo; Galeone, Aldo; Fusco, Alfredo; Pedone, Emilia; Luque Garriga, F. Xavier; Catalanotti, Bruno Abstract: UbcH10 is a component of the Ubiquitin Conjugation Enzymes (Ubc; E2) involved in the ubiquitination cascade controlling the cell cycle progression, whereby ubiquitin, activated by E1, is transferred through E2 to the target protein with the involvement of E3 enzymes. In this work we propose the first three dimensional model of the tetrameric complex formed by the human UbA1 (E1), two ubiquitin molecules and UbcH10 (E2), leading to the transthiolation reaction. The 3D model was built up by using an experimentally guided incremental docking strategy that combined homology modeling, protein-protein docking and refinement by means of molecular dynamics simulations. The structural features of the in silico model allowed us to identify the regions that mediate the recognition between the interacting proteins, revealing the active role of the ubiquitin crosslinked to E1 in the complex formation. Finally, the role of these regions involved in the E1-E2 binding was validated by designing short peptides that specifically interfere with the binding of UbcH10, thus supporting the reliability of the proposed model and representing valuable scaffolds for the design of peptidomimetic compounds that can bind selectively to Ubcs and inhibit the ubiquitylation process in pathological disorders. 2018-07-24T10:23:09Z Association of maternal weight with FADS and ELOVL genetic variants and fatty acid levels- The PREOBE follow-up. http://hdl.handle.net/2445/123832 Title: Association of maternal weight with FADS and ELOVL genetic variants and fatty acid levels- The PREOBE follow-up. Author: de la Garza Puentes, Andrea; Montes Goyanes, Rosa; Chisaguano Tonato, Aida Maribel; Torres-Espínola, Francisco José; Arias García, Miriam; de Almeida, Leonor; Bonilla Aguirre, María; Guerendiain, Marcela; Castellote Bargalló, Ana Isabel; Segura Moreno, Maite; García-Valdés, Luz; Campoy, Cristina; Lopez-Sabater, M. Carmen; PREOBE team Abstract: Single nucleotide polymorphisms (SNPs) in the genes encoding the fatty acid desaturase (FADS) and elongase (ELOVL) enzymes affect long-chain polyunsaturated fatty acid (LC-PUFA) production. We aimed to determine if these SNPs are associated with body mass index (BMI) or affect fatty acids (FAs) in pregnant women. Participants (n = 180) from the PREOBE cohort were grouped according to pre-pregnancy BMI: normal-weight (BMI = 18.5-24.9, n = 88) and overweight/obese (BMI≥25, n = 92). Plasma samples were analyzed at 24 weeks of gestation to measure FA levels in the phospholipid fraction. Selected SNPs were genotyped (7 in FADS1, 5 in FADS2, 3 in ELOVL2 and 2 in ELOVL5). Minor allele carriers of rs174545, rs174546, rs174548 and rs174553 (FADS1), and rs1535 and rs174583 (FADS2) were nominally associated with an increased risk of having a BMI≥25. Only for the normal-weight group, minor allele carriers of rs174537, rs174545, rs174546, and rs174553 (FADS1) were negatively associated with AA:DGLA index. Normal-weight women who were minor allele carriers of FADS SNPs had lower levels of AA, AA:DGLA and AA:LA indexes, and higher levels of DGLA, compared to major homozygotes. Among minor allele carriers of FADS2 and ELOVL2 SNPs, overweight/obese women showed higher DHA:EPA index than the normal-weight group; however, they did not present higher DHA concentrations than the normal-weight women. In conclusion, minor allele carriers of FADS SNPs have an increased risk of obesity. Maternal weight changes the effect of genotype on FA levels. Only in the normal-weight group, minor allele carriers of FADS SNPs displayed reduced enzymatic activity and FA levels. This suggests that women with a BMI≥25 are less affected by FADS genetic variants in this regard. In the presence of FADS2 and ELOVL2 SNPs, overweight/obese women showed higher n-3 LC-PUFA production indexes than women with normal weight, but this was not enough to obtain a higher n-3 LC-PUFA concentration. 2018-07-24T08:12:33Z Elevated circulating levels of succinate in human obesity are linked to specific gut microbiota http://hdl.handle.net/2445/123063 Title: Elevated circulating levels of succinate in human obesity are linked to specific gut microbiota Author: Serena, Carolina; Ceperuelo-Mallafré, Victoria; Keiran, Noelia; Queipo-Ortuño, Maria Isable; Bernal, Rosa; Gomez-Huelgas, Ricardo; Urpí Sardà, Mireia; Sabater, Mónica; Pérez-Brocal, Vicente; Andrés Lacueva, Ma. Cristina; Tinahones, Francisco J.; Fernández-Real, José Manuel; Vendrell, Joan; Fernández-Veledo, Sonia Abstract: Gut microbiota-related metabolites are potential clinical biomarkers for cardiovascular disease (CVD). Circulating succinate, a metabolite produced by both microbiota and the host, is increased in hypertension, ischemic heart disease, and type 2 diabetes. We aimed to analyze systemic levels of succinate in obesity, a major risk factor for CVD, and its relationship with gut microbiome. We explored the association of circulating succinate with specific metagenomic signatures in cross-sectional and prospective cohorts of Caucasian Spanish subjects. Obesity was associated with elevated levels of circulating succinate concomitant with impaired glucose metabolism. This increase was associated with specific changes in gut microbiota related to succinate metabolism: a higher relative abundance of succinate-producing Prevotellaceae (P) and Veillonellaceae (V), and a lower relative abundance of succinate-consuming Odoribacteraceae (O) and Clostridaceae (C) in obese individuals, with the (P + V/O + C) ratio being a main determinant of plasma succinate. Weight loss intervention decreased (P + V/O + C) ratio coincident with the reduction in circulating succinate. In the spontaneous evolution after good dietary advice, alterations in circulating succinate levels were linked to specific metagenomic signatures associated with carbohydrate metabolism and energy production with independence of body weight change. Our data support the importance of microbe-microbe interactions for the metabolite signature of gut microbiome and uncover succinate as a potential microbiota-derived metabolite related to CVD risk. 2018-06-18T13:28:01Z


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This property has been removed by the agent. It may be sold or temporarily removed from the market
3 bedroom end of terrace house for saleO'Connor Grove, Kirkby, Liverpool, Merseyside, L33
Property Description
Key features
- Three bedrooms
- Semi Detached
- Conservatory
- Garage
- Double glazed & central heated
- Gardens
Full description
Located in a quiet cul-de-sac this three bedroom semi detached home is well presented throughout. Comprising of Entrance hall, downstairs cloaks/WC, lounge, kitchen/ diner and conservatory to the ground floor. There are three bedrooms and family bathroom upstairs. Externally the property benefits from a detached garage and driveway and gardens. The property has great amenities and transport links with the M57 and East Lancashire Road being close by.
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Disclaimer - Property reference MAG170335. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by Entwistle Green, Maghull. Please contact the selling agent or developer directly to obtain any information which may be available under the terms of The Energy Performance of Buildings (Certificates and Inspections) (England and Wales) Regulations 2007 or the Home Report if in relation to a residential property in Scotland.
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This game uses pinyin and Chinese characters with no translation. The vocabulary is from YCT Level 1, with additional useful words and phrases. The two links below, which open in new windows ready for printing, are for the game, Wordsearch Ch1a with answers. This is designed for learners who are learning characters, including post 16.
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The town's grand list went up 2.19 percent this year, resulting in part from a new restaurant, an office building and a new way to assess motor vehicles.
It may seem like good news that the list of taxable property in the town is up from last year - meaning that revenue will increase about $200,000 - but the actual growth in real estate and personal property is slight.
"It's nice it went up, but it's minimal," said Mayor John F. Gauger Jr.
In all, the grand list grew to $297.81 million before exemptions, with the biggest growth showing in motor vehicles, which increased 21 percent, said Janice McKie, town assessor.
"It's only because we're assessing motor vehicles differently this year," McKie said, explaining that this year the vehicle's engine size and whether it has four-wheel-drive helps determine the value.
"While you can say you had an increase, you just changed the way you're counting beans," Gauger said.
In past years, the town determined a vehicle's value based on the average of the blue book value and the National Automobile Dealers Association guide. The value was determined by the make and model of a vehicle.
This year, though, the assessor is using a computerized guide that is able to determine the engine size and whether it has four- wheel-drive through the vehicle identification number, McKie said.
Town officials are bracing for a backlash from car owners who might be surprised to find that their vehicle assessments have increased when they receive their tax bills in July. "We're probably going to get a lot of repercussions," McKie said.


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Pub for saleHawk & Buckle, Llannefydd, Denbigh, LL16 5ED
Do you like this property?
Call: 0161 468 3952
Property Description
Commercial information
- Business for sale
Key features
- Impressive 17th Century semi-rural inn
- Lounge, public bar & restaurant: 80 covers
- Eight well-appointed en suite bedrooms
- 3 bed owner's accommodation
- Panoramic views to coast & Clwydian range
- Beer gardens: 30 & private parking: 8. Energy Rating D
Full description
Tenure: Freehold
A distinctive two storey double fronted 17th Century former coaching inn of colour rendered stone and brick elevations, beneath a multi-pitched tiled roofline having been thoughtfully extended over the years and now incorporating a total of eight en suite letting rooms, set upon a good sized plot with private beer patio and parking, affording panoramic views of the North Wales coastline and Clwydian range.Location
From the A55 North Wales coast road, take the exit for St. Asaph onto the B5381 turning left after the New Inn signposted Trefnant. After approximately two miles turn right, signposted Henllan onto the B5428 following this for a further 1.5 miles to the village of Llannefydd where the property can be found on the right hand side.Internal Details
Dating back to the 17th Century, the Hawk & Buckle offers a number of period features to include inglenook with a brand new log burner, beamed ceilings combined with modern amenities.
Comprising; entrance porch, large open-plan lounge bar (60) with a combination of leather sofas, free-standing tables and chairs, served by a good size bar servery to one wall.
Public bar with open grate fireplace and pool table.
Resident's restaurant and breakfast room (30) with tiled floor.
All fixtures and fittings are to be included within the sale however, any items that are owned by a third party or personal to our clients will be exempt.Ancillary Areas
Comprehensively equipped trade kitchen with adjacent food preparation / glass wash area, boiler room, rear porch, ladies and gents toilets, ground floor trade cellar and bottle store.Letting Accommodation
The property offers a total of eight en suite bedrooms of which five are on the ground floor with a further three en suite bedrooms at the first floor level.
The bedrooms are currently configured as luxury double rooms with spa bath, standard double rooms with en suite showers and family room with both bath and shower. All rooms offer free WiFi, TV and coffee/tea making facilities.
The property benefits from an excellent raised outdoor seating area with A-line tables affording far reaching views and private resident's parking for eight vehicles.Owner's Accommodation
Currently configured as: lounge, three bedrooms and a bathroom.Trading Information
Management accounts to 31 March 2018 show a net turnover of £101,846 with a gross margin of 63% and an adjusted net profit of £37,503.
Please note, these figures are based on extremely limited trading hours.
Bar opening hours:
Monday & Tuesday - closed
Wednesday to Friday - 6pm until close
Saturday - 4pm until close
Sunday - 6pm until close
The Rateable Value is £7,000 with effect from April 2017. Confirmation of actual business rates payable should be obtained from the local authority.Regulatory
Premises licence.
More information from this agent
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/commercial-property-for-sale/property-68395660.html
Energy Performance Certificate (EPC) graphs
Nearest station
- Abergele & Pensarn (5.6 mi)
BroadbandShow average broadband speed at this postcode Average broadband speed within this postcode Finding the fastest speed... Compare broadband deals
To view this property or request more details, contact:
How much will it cost me to call the number displayed on the site?
Standard geographic charges from landlines and mobiles apply and calls may be included in your telecom provider's call package.
To view this property or request more details, contact:
How much will it cost me to call the number displayed on the site?
Standard geographic charges from landlines and mobiles apply and calls may be included in your telecom provider's call package.
Map & Street View
Street View is unavailable in this location
Nearest station
- Abergele & Pensarn (5.6 mi)
To view this property or request more details, contact:
How much will it cost me to call the number displayed on the site?
Standard geographic charges from landlines and mobiles apply and calls may be included in your telecom provider's call package.
Disclaimer - Property reference 5651921-fh. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by Christie & Co , Manchester. Please contact the selling agent or developer directly to obtain any information which may be available under the terms of The Energy Performance of Buildings (Certificates and Inspections) (England and Wales) Regulations 2007 or the Home Report if in relation to a residential property in Scotland.
* Average speeds are based on the download speeds of at least 50% of customers at peak time (8pm to 10pm) from packages available on comparethemarket.com. Speed can be affected by a range of technical and environmental factors. The speed you receive where you live may be lower than that listed above. You can check the estimated speed to your property prior to purchasing. Fibre/cable services at your postcode are subject to availability. You can confirm availability on the provider's website. Providers may increase charges. You should have the right to exit your contract without penalty if this happens. The information is provided and maintained by Decision Technologies Limited.
Map data ©OpenStreetMap contributors.


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Oxford Playhouse is seeking two new Trustees to join the Board.
Anyone with an interest in theatre and the performing arts can apply for this important voluntary role overseeing the continued development and success of the Oxford Playhouse. We are particularly interested in recruiting Trustees with demonstrable expertise in one of the following areas:
For further information download an Application Pack here.
Deadline: 31st January 2019
Interviews: 7th February 2019


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Simple Steps for Starting Your Business: Module 1 - Startup Basics
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Business Plan Template for a Startup Business
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SCORE – Mentoring Northern Arizona's small businesses for 33 years
Small Business Crowdfunding Do's and Don'ts
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Developing a Business Plan
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