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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 812135, 9 pages
The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects
1The Laboratory of Molecular Pathology, Stem Cell Research Center, Children's Hospital of Pittsburgh, PA 15219, USA
2Department of Bioengineering, University of Pittsburgh, PA 15219, USA
3Department of Orthopedic Surgery, School of Medicine, University of Pittsburgh, PA 15213, USA
4Department of Pediatric Surgery, University of Texas, Medical School at Houston, TX 77030, USA
Received 30 June 2011; Accepted 11 September 2011
Academic Editor: Guy Benian
Copyright © 2011 Jianqun Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graft in vitro. The 3D graft created in vitro was then utilized to investigate muscle tissue repair in vivo. The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice. We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers. Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graft in vitro that can be employed for defect muscle tissue repair in vivo.
1. Introduction
Tissue loss usually results in significant dysfunction, physical deformities, and emotional pain long after treatment. Common causes of tissue loss include military injuries, such as high-velocity missiles, fragmenting exploding devices, and penetrating wounds associated with gunshots, and civilian injuries, such as blunt trauma attributed to automobile accidents [1]. Other causes of soft tissue removal include tumor removal, diabetic tissue damage, and irradiative injuries [2–5]. Current treatments of soft tissue injuries, such as skeletal muscle defects, resort to reconstructive plastic surgery, and skin grafting which are effective techniques to be employed in emergency repair and cosmetic applications, but often these techniques are unable to provide restoration of the function to the damaged tissue [6, 7].
Recent advances in biotechnology and bioengineering have offered novel approaches to repairing soft tissue defects by combining cell biology, gene transfer, biomaterials, and bioreactors [8–12]. Among the new advances, three-dimensional biodegradable scaffolds provide the ability to restore form and also function to the injured area. In order to be successful, the 3D biodegradable scaffold needs to serve two purposes. First, the scaffold needs to have the physiologic and mechanical properties that mimic the in vivo native environment, and second, the scaffold needs to have the ability to allow for development and remodeling of the tissue in order to promote successful restoration of physiological function [13–15]. It is important for the scaffold to be biodegradable because nondegradable constructs implanted for prolonged time periods often induce an inflammatory response which can often compromise the tissue construct and the healing of the tissue [16–18].
Tissue and organ function are dependent on the presence of appropriate populations of differentiated cells, and therefore, it is important to incorporate proper cells in the 3D scaffold. This can be accomplished through rolling bottle cell culture systems which are advantageous, in comparison to static cell seeding, in that a rolling bottle cell culture system improves cell seeding density and homogeneity within the scaffold [10, 19, 20]. This is accomplished through the slow revolutions of the culture bottles on the rolling device which produce a force significant enough to permit the cells to grow evenly in three dimensions [21]. Also the revolutions allow for nutrients to be provided to the cells while at the same time removing waste products through the constant movement of media bathing the cells [22].
In general, the artificial 3D scaffold provides an architecture on which seeded cells can organize and develop into the desired tissue for implantation. Once implanted in the soft tissue defect, the biodegradable scaffold provides an initial biomechanical structure for the replacement tissue until the cells produce an adequate extracellular matrix. During the deposition, organization, and formation of the newly generated extracellular matrix, the scaffold is either degraded or metabolized; eventually leaving a vital organ or tissue that restores, maintains, or improves tissue function. In this study, we built a 3D muscle graft by seeding C2C12 myoblasts into a biodegradable 3D collagen composite scaffold, and implanted it into a skeletal muscle defect mouse model in vivo. Immunostaining was employed to evaluate the effectiveness of the graft in improving vascularization, innervation, and regeneration of the damaged tissue.
2. Materials and Methods
2.1. C2C12 Myoblasts Transfected with Retroviral LacZ and LacZ Staining
C2C12 myoblasts were purchased from ATCC (American Type Culture Collection) and cultured in media (DMEM, Invitrogen) supplemented with 10% fetal bovine serum (FBS, Gibco, BR), 10% horse serum (HS), 1% penicillin/streptomycin (P/S), and 0.5% chick embryo extract (CEE). The culture media was removed and replaced with a 1 : 1 dilution of retroviral LacZ and culture media supplemented with 8 g/mL of polybrene. The cells were incubated in the solution for 6 hours at 5% CO2 and 37°C. To ensure maximum transduction of the virus, the infection was done up to 3 times, and a LacZ staining was done after each infection to determine the efficiency of the infections. The LacZ staining consisted of fixing the cells in 1% glutaraldehyde solution (Sigma) for 2 minutes followed by washing with PBS. The cells were then incubated for 2 hours in a LacZ solution (225 mL PBS/25 mL KFE solution/250 L of 2 M MgCl2/5 mL of X-gal) at 37°C. The cells were then visualized for analysis using bright field microscopy.
2.2. 3D Collagen Composite Scaffold and Cell Seeding
The 3D collagen composite scaffolds were purchased from BD Biosciences, (catalogue number 354613) and the scaffolds are of a cylindrical shape approximately 4.2–5.2 mm in diameter and 3.9–4.5 mm in height with a volume of 0.039 cm3. The average pore size of the scaffold is 100–200 μm and the hydration capacity is 25 L. The weight and wet weight of the scaffolds are 3.5 mg and 45.0 mg, respectively, with an average wet tear strength of 0.48 ± 0.0131 lb/mm. The collagen composite scaffolds, derived from bovine hide, resemble the structure of collagen within the extracellular matrix, and are able to support both short- and long-term differentiation of multiple cell types. The 3D scaffolds were individually placed into separate wells of a 48-well plate, and were immersed in a solution consisting of 200 L of culture medium containing approximately 50,000 LacZ+ myoblasts. The scaffolds were then incubated in 37°C at 5% CO2 for 1 hour.
2.3. Rolling Bottle Cell Culture System
The LacZ+ myoblast seeded scaffolds were transferred from the 48-well plates into Corning cell culture bottles containing 200 mL of culture media. The Corning cell culture bottles were placed on a Wheaton rolling device (Fischer Scientific) at a speed of 5 revolutions per minute (RPM). The Wheaton rolling device was then placed in an incubator at 37°C and 5% CO2 for 3 weeks with periodic cell culture media changes.
2.4. Scaffold Sectioning and Immunostaining
In vitro, the scaffolds were harvested after 1, 2, and 3 weeks of culturing, and rinsed with PBS before being snap-frozen in 2-methyl butane precooled in liquid nitrogen and stored at −80°C. The scaffolds were then cryosectioned at 10 μm and prepared for immunostaining.
In vivo, scaffolds were harvested at various time points, and immunostaining was performed to detect CD31, vWF, dystrophin, neurofilament protein, and fast MyHC expression in injured GMs implanted with the myoblast seeded scaffolds. Tissue sections were fixed in 4% formalin for 5 minutes followed by washing with PBS. Nonspecific binding was blocked with 10% HS for 1 hour at room temperature (RT). Primary antibodies were diluted to 1 : 200 in 2% HS and incubated overnight at 4°C. The sections were then rinsed with PBS followed by secondary antibody incubation for 1 hour at RT in a 1 : 300 dilution. The sections were then rinsed with PBS before the nuclei were counterstained with DAPI.
2.5. Proliferation Assay
After 10 days of culturing the scaffolds in the Wheaton rolling device, a BrdU assay was performed in order to evaluate the proliferation capacity of the C2C12 myoblasts within the scaffold. BrdU was added to growth medium at dilution of 1 : 1000 for an incubation period of 24 hours. The scaffolds were then removed from the medium and rinsed with PBS before being snap-frozen in 2-methyl butane precooled in liquid nitrogen. The scaffolds were then sectioned and fixed with 4% formalin. The DNA was denatured for 10 min on ice with 1 N HCl followed by 2 N HCl at RT for 10 min, and finally 2 N HCl for 20 min at 37°C. Nonspecific binding was blocked with 10% HS for 1 hour. The primary antibody (biotin conjugated anti-BrdU) was added at a 1 : 250 dilution in 2% HS for 3 hours at RT. The secondary antibody (biotin streptavidin) was then added at a dilution of 1 : 400 for 1 hour at RT. The nuclei were then counterstained with DAPI.
2.6. Animal Experiments
The animal protocol was approved by the Animal Research and Care Committee at Children's Hospital of Pittsburgh. The mice included normal (C57BL6J, 5–8 weeks of age, male), SCID (C57BL/6J-Prkdcscid/SzJ, 4–8 weeks of age, female), and dystrophic/immunodeficient mdx/SCID (C57BL/10ScSn-Dmdmdx crossed with C57BL/6J-Prkdcscid/SzJ, 4–8 weeks of age, female). All mice were purchased from Jackson Laboratory (Bar Harbor, Maine), and were housed individually with proper access to food and water and maintained on a 12-hour light/dark cycle. After the mice were anesthetized, an approximately mm3 defect was created in the belly of the gastrocnemius muscle (GM). The 3D muscle grafts and control nonseeded scaffolds were implanted in the defects followed by immediate wound closure. At designed time periods, mice were sacrificed, and the GMs were harvested and snap-frozen in 2-methylbutane precooled in liquid nitrogen, and they were cryosectioned for histological analysis.
3. Results
3.1. In Vitro
In the in vitro portion of the experiments, the C2C12 myoblast seeded scaffolds were cultured in a roller bottle cell culture system, and the scaffolds were harvested after 1, 2, and 3 weeks of culturing. After 1 week of culture, myoblasts were distributed throughout the interior of the scaffold which provides evidence that extensive migration was able to occur (Figures 1(a)–1(f)). Some of the cells migrated into the interior region of the scaffold, and this can be detected by either HE (Figures 1(a)–1(c)) or LacZ (Figures 1(d)–1(f)) staining. The sectioned scaffolds were also immunostained for CD31, an endothelial marker, and vWF, an endothelial cell-specific protein (Figures 2(a)–2(c)). After 1 week of culture, CD31positive cells were seen within the scaffold (red, Figure 2(a)). Colocalization between CD31-positive (red) and vWF-positive (green) cells was visualized after 2 weeks of culture (yellow, Figure 2(b)). The detection of the colocalization of CD31 positive and vWF positive cells is indicative of vascular development within the scaffold. After 3 weeks of culturing, more extensive colocalized positive cells were seen (yellow, Figure 2(c)). A BrdU assay was performed in order to assess the viability and proliferative capacity of the myoblasts within the scaffold. We detected myoblasts with nuclei positive for BrdU (red, Figures 2(d), 2(e), and 2(f)), revealing that some of the myoblasts seeded within the scaffold were able to proliferate.
3.2. In Vivo
In the normal mouse model, implantation of a nonseeded control scaffold resulted in rejection from the host tissue, and this was seen 20 days after implantation (Figure 3(a)). In the SCID mouse model, the implanted nonseeded scaffolds were not rejected by the host tissue, but few host cells migrated into the area of the implantation (Figure 3(b)). In the same SCID mouse model, implantation of the 3D muscle graft with LacZ-positive myoblasts for a duration of 20 days, resulted in detection of large populations of LacZ-positive cells remaining in the transplantation site as well as extensive migration of the LacZ-positive cells into the surrounding host tissue, which demonstrates the success of the 3D graft implantation into the host tissue (Figure 3(c)). With immunostaining, we discovered CD31 and vWF positive cells present within the interior of the scaffold, and some of these cells expressed colocalization of the two markers (Figure 3(d)), indicating that the vasculature process had began to develop.
In the mdx/SCID mouse model, we followed the same procedure described above for the normal mouse model. We observed that the trauma sites were quickly healed in the 3D muscle graft implanted skeletal muscle after 10 (Figure 4(a)) and 20 days (Figure 4(c)) after surgery in comparison to the healing seen in the nonseeded scaffolds implanted into the muscle (Figures 4(b) and 4(d)). By histological analysis, the LacZ-positive cells were able to survive within the implanted scaffold as well as migrate throughout the scaffold. The scaffold began to breakdown as it was incorporated within the surrounding host tissue 10 days after implantation (Figures 4(e) and 4(f)). Twenty days after the graft implantation, we also detected that multiple cell types were spread homogeneously throughout the site of injury, and significantly, we detected CD31 and vWF positive cells present in the new tissue and some of these cells demonstrated colocalization between these two markers (Figures 4(g) and 4(h)). This result indicates that the vascularization process had started within the 3D muscle graft as early as 10 days after implantation.
We also investigated the histology of the long-term 3D muscle graft after implantation in the mdx/SCID mouse model. One month after implantation of the 3D muscle graft, LacZ and eosin staining provided evidence that the LacZ-positive myoblasts were able to survive within the implanted scaffold (Figure 5(a)). The site of injury was composed of LacZ-positive myoblasts and nondegraded residues of the scaffold (Figure 5(a) and 5(c) arrows). Some LacZ-positive myoblasts were detected at the periphery of the scaffold forming LacZ positive multinucleated myotubes (Figure 5(a), arrowheads). Many desmin positive cells (arrowheads), indicative of mature muscle cells, were detected within the area of implantation around nondegraded regions of the scaffold (Figure 5(b), arrows). With an H&E staining, we detected newly formed myofibers within the region of the degrading scaffold (Figure 5(d)). With immunostaining, dystrophin-positive myofibers were detected within the implantation region which suggests that regeneration was occurring within the site of injury (Figure 5(e), arrowheads). We also discovered that some CD31 positive cells were located within the site of injury, revealing that the vascularization was an ongoing process (Figures 5(e) and 5(f), arrows).
Two months after implantation of the 3D muscle grafts, large dystrophin positive grafts were detected within the site of injury suggesting that muscle regeneration had taken place in many regions of the injured area (Figures 6(a) B, E, and H). Also, the majority of the dystrophin-positive grafts stained positive for fast myosin-heavy chain protein (Figures 6(a) G–I). Similar to the previous time points, CD31 was visualized which provides evidence for extensive vascular development within the site of injury (Figures 6(a) A–C). Also neurofilament protein was detected within the dystrophin-positive grafts suggesting that innervations were being formed to the regenerating myofibers (Figure 6(a) D–F). With histological analysis, we have detected a large area of scar tissue remaining in the implanted 3D graft sites three months after implantation through an H&E staining (Figure 6(b) A and B, stars). Also, we have detected regeneration ofmyofibers (Figure 6(b) B, arrows) and large amounts of dystrophin-positive myofibers within the new muscle graft (Figure 6(b) C and D, dystrophin-red, arrows) with revascularization (Figure 6(b) D, CD31-green, arrowheads). This existing scar tissue may be a major factor to interfere with further muscle functional recovery, and can be a potential inducer of muscle reinjury with movement. Thus, prevention of fibrosis is necessary for the success of the bioengineered tissue in application.
4. Discussion
In the last few decades, advances in bioengineering have moved the field of tissue engineering significantly forward [15, 23, 24]. While 3D tissue constructs are still being developed, this field continues to grow from the intense efforts of military and civilian trauma researchers. Tissue engineering techniques combined with cell biology and material sciences hold great promise in developing appropriate strategies for the repair and regeneration of biological tissues. The necessary components for successful 3D engineered tissues have been identified to include the following: a biodegradable material that serves as a scaffold to facilitate new tissue growth, and pluripotent or stem cells to initiate new tissue growth as well as promoting vascularization and innervation once implanted within the patient. Our work focused primarily on creating functional 3D tissue constructs in vitro, through the use of a 3D biodegradable scaffold seeded with C2C12 primary myoblasts, which could then be implanted in vivo for the purpose of repairing and replacing damaged and lost tissue due to soft tissue defects.
Our results confirmed that C2C12 myoblasts are able to survive and migrate within the 3D collagen composite scaffold in vitro. This is a necessary step to occur prior to implantation in order to ensure that the implanted scaffolds are incorporating a rich source of myoblasts into the area of the skeletal muscle injury. Also, we discovered that in vitro, CD31-positive cells were present after 1 week of culturing. CD31 is an endothelial marker that signifies progenitor blood vessel cells [25, 26] which means that the cell seeded scaffold had already started to develop some form of vasculature prior to implantation. This is furthered by evidence that after 2 weeks of culturing in vitro, colocalization between CD31- and vWF-positive cells was detected. These results indicate that the cell seeded scaffolds are capable of forming a type of prevascularization which is ideal for inducing vascular development within the defected muscle once implanted (Figure 7).
The ability to promote vascularization is an essential aspect of tissue engineering because often the vascularization to the area where the tissue loss took place is compromised by the trauma itself [4]. The vascularization that was seen in vitro was also present when the scaffolds were harvested at various time points after implantation in vivo. The detection of CD31-positive cells located throughout the scaffold provide evidence that vascularization was being incorporated within the scaffold from vessels located in the host tissue surrounding the defect. This incorporation of vasculature within the scaffold increased the cell survival of the myoblasts by providing nutrients and oxygen to the newly transplanted cells [27, 28]. In order for a tissue construct to be effective in becoming functional, vascularization needs to occur along with innervation. Techniques for repairing soft tissue defects, such as muscle and perforator flaps, result in providing vascularized coverage to the wound; however, due to the lack of innervations to the damaged area, sensitivity is often compromised [6]. Our results indicated that the seeded scaffold was successful in providing potential innervations to the damaged tissue based on the presence of neurofilament-positive cells. Neurofilament protein is a protein located in the intermediate filament of neurons [29]; therefore, its expression is indicative of neurogenesis and neural development.
Dystrophin-positive myofibers were found located within the area of the defect which signifies that new muscle fibers were being formed in the damaged area. The myoblasts that were seeded in the scaffold were able to successfully differentiate into myotubes which allowed for regeneration of the damaged tissue. This result is not surprising due to numerous previous studies that found that myoblast transplantation is an effective therapy for increasing dystrophin-positive myofibers in such muscle dystrophy diseases such as Duchenne muscular dystrophy [30–32]. A large quantity of the dystrophin-positive myofibers stained positively for fast-type myosin-heavy chain protein, and a lower quantity staining positively for slow-type myosin-heavy chain protein. This preferential difference may be contributed to the environment into which the myoblasts were implanted [33]. If more fast-type myosin-heavy chain myofibers were present in the surrounding area of host tissue around the defect, differentiation of the myoblasts into myotubes exhibiting fast-type myosin-heavy chain proteins may have been favored.
Although many challenges still remain in the successful restoration of soft tissue defects, our study provides a starting point for overcoming these challenges. The ability of the myoblast seeded collagen composite scaffolds to provide the initial architecture for rebuilding a functional tissue, as well as incorporating an appropriate population of cells that helps to facilitate muscular regeneration through increasing innervation, vascularization, and the production of myofibers, makes the 3D collagen scaffold an ideal candidate for repairing soft tissue defects. However, immunocompatibility and fibrosis are two problems that still remain. As seen in our data, only immune-compromised mice were able to successfully integrate the 3D tissue graft into their host tissue which presents a limited scope of application for the scaffolds. Also, fibrosis is a pathological process that affects many tissues and organs after being afflicted by injuries or disease-like states [34]. The development of the fibrotic tissue can lead to chronic healing problems, which can result in tissue/organ dysfunction during the final healing stages, and can make the tissue more susceptible to reinjury after initial healing [35, 36]. Our study detected fibrosis formation in the skeletal muscles after long-term implantation, indicating that prevention or treatment of the fibrous scar tissue is essential for effective bioengineered tissues.
Author's Contributions
J. Ma and K. Holden contributed equally to this paper.
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I haven't posted anything in so long, and as much as I'd like to use some witty or funny excuse about how I met a guy and we're getting married, and I've moved to another country so my life is super hectic right now – oh wait. That's exactly what's happening.
But, here it is. The shoe post that's been, to be honest, sitting in my drafts and just waiting for me to get off my ass and take some pictures of my shoes!
Continue reading "Walk a Mile in my Shoes"

subset: commoncrawl


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* Death toll rises to at least 877 in Haiti
* Storm gradually weakening off U.S. coast - NHC
* Mass evacuations in four U.S. states
Hurricane Matthew killed almost 900 people and displaced tens of thousands in Haiti before plowing northward on Saturday just off the southeast U.S. coast, where it caused major flooding and widespread power outages.
The number of deaths in Haiti, the poorest country in the Americas, jumped to at least 877 on Friday as information trickled in from remote areas previously cut off by the storm, according to a Reuters tally of tolls from officials.
Matthew rampaged through Haiti's western peninsula on Tuesday with 145 mph (233 kph) winds and torrential rain. Some 61,500 people were in shelters, officials said, after the storm hurled the sea into fragile coastal villages, some of which were only now being contacted.
At least three towns in the hills and coast of Haiti's fertile western tip reported dozens of people killed, including the farming village of Chantal where the mayor said 86 people died, mostly when trees crushed houses. He said 20 others were missing.
"A tree fell on the house and flattened it. The entire house fell on us. I couldn't get out," said 27-year-old driver Jean-Pierre Jean-Donald.
"People came to lift the rubble, and then we saw my wife who had died in the same spot," said Jean-Donald, who had been married for only a year. His young daughter stood by his side, crying "Mommy."
With cellphone networks down and roads flooded, aid has been slow to reach hard-hit areas in Haiti. Food was scarce and at least seven people died of cholera, likely because of flood water mixing with sewage.
The Mesa Verde, a U.S. Navy amphibious transport dock ship, was en route to Haiti to support relief efforts. The ship has heavy-lift helicopters, bulldozers, fresh-water delivery vehicles and two surgical operating rooms.
At 5 a.m. EDT (0900 GMT), Matthew's eye was about 20 miles (30 km) south-southeast of Hilton Head, South Carolina. It was moving northward at 12 mph (19 kph), packing 105 mph (165 kph) winds, the U.S. National Hurricane Center said.
INSANE FOOTAGE of debris flying across streets in Daytona Beach, Florida, as Hurricane #Matthew moves along the coast via @CNN pic.twitter.com/CSAp99XSf7— Aaron Vallely (@Vallmeister) October 8, 2016
Video shows flooding in St. Augustine, Florida, as a result of Hurricane #Matthew https://t.co/jljBSZcdMz pic.twitter.com/LizzmdhbjS— CNN (@CNN) October 7, 2016
It reported wind gusts of 80 mph (130 kph) in Hilton Head and predicted the storm would possibly striking the coast on Saturday morning or afternoon.
"Regardless of whether or not the center makes landfall, hurricane-force winds in the northern eyewall will lash much of the coast of South Carolina," an NHC advisory said.
Matthew sideswiped Florida's coast with winds of up to 120 mph (195 kph) but did not make landfall there. The storm was a Category 2 on the five-step Saffir-Simpson scale of hurricane intensity. Category 5 is the strongest.
There were at least four storm-related deaths in Florida but no immediate reports of significant damage in cities and towns where Matthew swamped streets, toppled trees and knocked out power to about 1 million households and businesses.
About 450,000 were without power in Georgia and South Carolina on Saturday, according to utility companies.
In Florida, two people were killed by falling trees and an elderly couple died of carbon monoxide poisoning from a generator while sheltering from the storm inside a garage.
Hurricane and flash flood warnings extended through Georgia and South Carolina and into North Carolina early on Saturday.
Forecasters warned of flooding as 15 inches (40 cm) of rain were expected to fall in parts of the region along with massive storm surges and high tides.
Several major roadways were inundated in Charleston, South Carolina, local media reported.
Standing water closed both directions of the Interstate 95 highway in Georgia. Some 8 inches (20 cm) of rain had fallen in the Savannah, Georgia area where Matthew downed trees and caused flooding of streets.
Large parts of a hotel coming off in Daytona Beach, Florida. #HurricaneMatthew#Matthew pic.twitter.com/54WZtv0lnV— Silver Surfer (@RobPulseNews) October 7, 2016
WATCH: Storm surge from Hurricane Matthew brings significant flooding to St. Augustine, Florida. pic.twitter.com/upslTTPWy0— NBC Nightly News (@NBCNightlyNews) October 7, 2016
Hurricane Matthew Damage: First look at damage along the Florida coast #FLwx #Matthew pic.twitter.com/4OYXYcNkFo— WeatherNation (@WeatherNation) October 8, 2016
Though gradually weakening, Matthew - which triggered mass evacuations along the U.S. coast - was forecast to remain a hurricane until it begins moving away from the U.S. Southeast on Sunday, according to the NHC.
President Barack Obama and officials urged people to heed safety instructions. Coastal residents were warned that storm surges could still pose a danger by flooding entire neighborhoods even as Matthew departs the region.
Banner and thumbnail credit: Reuters, Henry Romero


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February 25, 2013 12:00 ET
LONDON, UNITED KINGDOM--(Marketwire - Feb. 25, 2013) - There's nothing quite like a mother's love - unconditional all year around, so Mothering Sunday is the one time of the year we can stop and make time for our mothers to thank them for their devotion. In celebration of this, ChuckleMonster, an online retailer of cards for all occasions, is lending a helping hand with some Mother's Day offers and a Twitter competition.
Some might opt for funny Mother's Day cards, while some might want sentimental, others simply choose cute. Whatever the preference, the card selection from ChuckleMonster can be personalised to suit and it is offering all medium-sized cards at just £1.99 from now until Mothering Sunday on 10th March, with large cards at £4.99. In addition, a ReTweet competition is being held where a lucky winner will be able to choose a personalised card for their mum, which will be sent along with flowers.
To enter, simply follow Chucklemonster on Twitter @Chuckle_Monster and RT any of the Mother's Day competition posts.
John Phipps, E-Commerce Director at ChuckleMonster says: "We all know we should show our mums appreciation throughout the year, but with busy lives and other commitments, Mothering Sunday is the one dedicated time in the year to show your mum you care and give her some TLC, so we thought we'd lend a hand with our ReTweet competition on Twitter and some great card offers.
"Some might take their mum for a meal, treat her to some chocolates or flowers or send her off on a pampering spa day. Whatever the treat or gift, a card is a treasured item that mums will keep year on year, so extra time and care should be given to choosing one that conveys feelings and makes all the difference."
Personalised cards are increasingly popular, with various options such as photos and bespoke messages the choice is endless and people can really be sure when they go personalised that their card is a real one-of-a-kind.
For those who might not be able to make the journey on Mothering Sunday, a card can be sent directly to mum so she doesn't miss out. ChuckleMonster provides an easy, fuss-free way of celebrating all occasions, whether it's sending anniversary cards, personalised birthday cards or a simple thank you message.
To see the extensive range of card options from ChuckleMonster, visit www.chucklemonster.com.
Georgina Dunkley01858 firstname.lastname@example.com
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This is, strongly, how I view the Tigers and Red Wings in rebuilding mode: It's necessary, and therefore welcome.
Dylan Larkin and Anthony Mantha aren't stars yet, that's for sure, but they could be. So this is the summer of Jeimer. So what? Maybe this Candelario kid can play.
It should be understood pretty much every big success by this town's professional sports teams, at least in the not-too-distant past, started under the similar circumstances.
The 1984 Tigers were constructed gradually from the demise of the 1968 World Series champs. By 1975, the Tigers were MLB's worst team. By 1978, they had a winning record, and soon became contenders in a very strong American League East, eventually winning it all.
It was because of drafting exceptionally well and solid player development. Jack Morris and Alan Trammell will be inducted into the Baseball Hall of Fame this summer. Lou Whitaker should already be in. Kirk Gibson, Lance Parrish, Dan Petry and Tom Brookens were homegrown. So were first-round draft picks Steve Kemp and Glenn Wilson, significant pieces because they were flipped into key parts in trades for Chet Lemon and Willie Hernandez.
The Pistons were next up. They were a bad team, which began to turn it around by drafting Isiah Thomas second overall. They also drafted Dennis Rodman, John Salley and Joe Dumars. They traded for pieces such as Bill Laimbeer, Rick Mahorn, James Edwards and Vinnie Johnson. Kelly Tripucka was drafted, had early success, was traded for Adrian Dantley, who was dealt for Mark Aguirre. Rebuild-became-contention-led-to-championships.
The Red Wings followed a similar path. A lot of young, homegrown talent emerged. The genesis of the Red Wings' back-to-back Stanley Cup championships in 1997 and 1998 was drafting wisely and superior player development. Nicklas Lidstrom and Sergei Fedorov are in the Hockey Hall of Fame. Neither was a first-round draft choice. The drafting of Darren McCarty, and astute trades for young players Kirk Maltby and Kris Draper led to the Grind Line. And like the Tigers, the Red Wings used their young talent in trades to complete the process, most notably Keith Primeau as a key element to acquire Brendan Shanahan.
No question, there were growing pains during those periods, and monumental disappointments. The Tigers were ousted in a close race in 1981, and had difficulty breaking through against stiff competition. The Red Wings were swept by New Jersey in the 1995 Stanley Cup finals, and ousted by the Avalanche after dominating the regular season the following campaign.
Oh, and the Pistons. "There's a steal by Bird." And it was so obvious Laimbeer didn't foul Kareem in the NBA Finals…
The Going to Work Pistons were built mostly on veteran talent acquired in a variety of ways, but it all started under what appeared to be dire circumstances - losing resident star Grant Hill. It resulted in his sign-and-trade to Orlando. One of the players coming back was a young Ben Wallace.
The struggles only made it sweeter when the above-mentioned teams ultimately reached the mountain top. It was especially welcome considering how this town came to identify with the stars since they literally watched them, and their teams collectively, grow before their eyes.
Rebuilds can get out of control. The Tigers had a dozen straight losing seasons during the 1990s and 2000s. The Pistons have essentially been a wreck for a decade now. The Red Wings should have started their current process sooner.
The thing with young players is you just don't know if they will be the next Tram and Jack or Deivi Cruz and Brian Moehler.
But at least an influx of still-developing talent eventually gives a franchise a chance. The Pistons are still just spinning their wheels.
By acknowledging their circumstances, the Tigers and Red Wings have an opportunity to turn it around, if not soon, at least some day.


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Inn at Orchard Heights
- 20 Church Street
- New Paltz, NY 12561
- (845) 255-6792
- Visit Site | E-Mail
- Details
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Our beautiful 1888 Queen Anne Victorian home boasts spacious bedrooms with both private and shared baths. There are comfortable dining areas, a bright sunny parlor, inviting sitting areas, porches and an outdoor garden for relaxing or eating. At Orchard Heights you can enjoy understated elegance in a cozy setting. Our property offers beauty and privacy with seasonal mountain views.
B&B & Country Inns & Farm Stays
- B&Bs & Country Inns:


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We have changed our domainWe have changed servers and operate Career|edu under a different domain. Our services will remain unchanged and we will continue to post the best career openings for the academic world at our new domain.
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Assistant Professor Production SystemsPosted by: University of Twente (UT)
Posted date: 2017-Nov-24
Location: Netherlands
Production systems is about transforming resources into useful products and service. It includes both the technical as the organizational aspects. Hence, the ambition of University of Twente, from 'high tech' to 'human touch', provides the excellent breeding ground to study production systems, as its concerns lie in making artefacts available in a useful, efficient and meaningful way to the individual, organizations, and society at large. In considering production systems as the transformers of scarce resources into most known man made products, production system design and research is at the core of the transition to Smart Industries. And in relation to that, at the UT all aspects including socio-technical, marketing, systems engineering, ITC, sensors and control are present.
The chair of Production Systems is one of the core pillars of the mechanical engineering and industrial design engineering programs at the University of Twente. As such, the chair is responsible for a variety of courses, projects and educational renewal initiatives. The design programs reflect the university's multidisciplinary focus, its entrepreneurial character and also honour the potential of new technologies and smart designs for both industrial applications and for enriching people's everyday experiences.
The research of the chair of Production Systems focuses on the understanding and improvement of production systems and the related design processes for such systems. In this, the development of design support tools and knowledge substantiates the application oriented approach. The chair has a foundation in applying design to advance the efficiency and effectiveness of production systems. A core thrust of the chair is to bring together factory physics and design knowledge, e.g. design methodologies, in order to synthesize new technologies and scientific insights in applications for production systems. The research of the chair of Production Systems involves design for smart production systems, ranging from synthesis approaches and tools, via the development of Smart Industry (Industry 4.0) and Lean manufacturing concepts to addressing more general production management oriented challenges. The manufacturing research involves the holistic consideration of products, production, and product lifecycles, based on sophisticated and groundbreaking modelling methods and information driven technologies for production environments.
The challenge
Teaching: As assistant professor you will contribute to Production System Courses in our Bachelor and Master program in Mechanical Engineering, and to the education/supervision in our program for Professional Doctorates in Engineering. Also, you will supervise BSc- and MSc-thesis projects. The total teaching load will be about 50%. If you are not yet formally qualified for teaching at universities, we offer a program and mentoring to obtain this qualification within 2 years (University Teaching Qualification, UTQ).
Research: We expect you to work as an independent researcher able to identify the academic questions in practical applications related to Production Systems, Smart Industries and the crossroads of design and engineering. You are able to bridge between academic research results and engineering practice and publish research results in high quality international refereed journals. You also have a positive attitude towards building networks and attracting external funding (national science foundation, EU, government and industry) for junior researchers (PhD and PDEng positions), of whom you will become the daily supervisor.
Are you up for the challenge to improve the world with your research as well as teaching on this state of the art technology?
Information and application
Interested candidates can upload their application, including a motivation letter, CV, a full publication list and two references by link below, before December 19th 2017 through the link below.
Conditions of employment
You will initially be employed for a period of 2 years, with the prospect of a permanent position after a positive evaluation. Salary and conditions are in accordance with the Collective Labour Agreement (CAO) of the Dutch Universities. Gross monthly salary is between € 3.111,- and € 4.757,- depending on your experience and qualifications. In addition, the University of Twente offers attractive employment conditions (for example 8% holiday allowance and 8,3% end-of-year bonus), excellent support for research and facilities for professional and personal development.
The University of Twente. We stand for life sciences and technology. High tech and human touch. Education and research that matter. New technology which leads change, innovation and progress in society. The University of Twente is the only campus university of the Netherlands; divided over five faculties we provide more than fifty educational programmes. We have a strong focus on personal development and talented researchers are given scope for carrying out groundbreaking research.
We are an equal opportunity employer and value diversity at our company. We do not discriminate on the basis of race, religion, color, national origin, gender, sexual orientation, age, marital status or disability status. Because of our diversity values we do particularly support women to apply.


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Do you belong to the club of bargainers; always wanting value for your money? Are you an offer freak? I bet you are continuously looking into deals where you can get quality with a cheap price tag. I don't blame you.
I don't know but for me, there is a thrill I get in having the touch and feel of a hardcopy; especially when it comes to magazines and pictures. Being the old fashion type, I don't really embrace having copies of my legal documents and memories on a flash drive or kept away "safely" on Google Drive. What happens if you lose all your data by a simple mistake of clicking the wrong button! Well, that's why it's always important to make hard copies. What better way than the use of print! Continue reading


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When I was opening said pressie I spotted the little Love Hearts logo and got embarrassingly exicted. Then I saw lip gloss...and perfume...and body lotion!! By this point I was hyperventilating and my 25 year old self was telling my increasingly excited 8 year old self to keep cool and at least try to refrain from squealing like a girl. Epic fail :)
Strawberry Dream Eau de Toilette
You probably wont be too surprised to hear that this is seriously sweet and smells of (fake) strawberries which I rather like! It lasts forever and you only need the teensiest little spritz. My kid brothers love the smell and my dad hates it – if we needed proof that this is aimed at the younger generation then I think this is it
Simple, glitterly, slightly sticky but definitely not unpleasant. Generally I wear the pink shade cos it's subtle but still really pretty. Every time I've applied this in the bathroom at work someone has asked me where I got it. I had no idea people loved Love Hearts so much!
Talking of which I got my very first piece of Christmas tat of the year. It's a rubber duck with a little santa hat and a candy cane and I just think it's beyond cute. I really am 25 you know. Honest :)
Sometimes I cant help but put something aside for a "special occasion" which is exactly what I've done with this. Because I'm not using it yet I havent actually opened it to see how it smells. I've decided it's going to be my Christmas body lotion so I'll be using it on the run up to the big day!


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Lila Downs - Y la Misteriosa En Paris: Live at Fip
- Jan Fairley
- 21 April 2010
(World Village)
What could be more glorious than a live recording of a Lila Downs' gig in Paris? The Latin American songwriter has revamped Mexican traditional music for the 21st century, embedding it in indigenous languages and the power of the feminine, while infusing it with humanitarian politics. Here, with her multi-national South American band she gives her all to classics like 'La Iguana', her re-working of 'La Cucaracha' and its penchant for marijuana, as well as a gypsified 'Perro negro'.


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I have to say, I'm pleasantly surprised by how many of your comments on this week's giveaway were pro-pillow fight. Though I agree certain conditions (like who's swinging, where they're aiming and how heavy the pillow is) can make all the difference. Just promise us that any pillows won or purchased from are treated less like weapons and more like the stylish accents that they are.
As you know, Oliver is giving away one pair of matching pillows featuring any of the fabrics on . And while we're obviously not eligible for the prize, we're looking forward to checking out their selection in person tonight at their new store in Richmond (5612 Grove Avenue, if you're interested). Though you'll have to promise to behave and not start any impromptu pillow battles… unless it's the kind we're you're fighting over a favorite discounted fabric sample.
So who's claiming the first prize of 2010? Well, after I wooed random.org with a mixed tape of jams from the Alanis era, it said we oughta know that our winner is… Nicole (for whom a normal pillow fight isn't enough, she prefers them at hotels where jumping on the bed is also part of the equation). Congrats! Now for the difficult decision of picking your favorite fabric (don't worry, you can pick two if you want to do a coordinating front and back design, that way it's more like you get four free pillows and two totally different looks).
And try not to be too bummed if you didn't win this time. Next week's prize is pretty great too. It's a little something that we fell in love with last year- so much so that we actually sent it to everyone on our Christmas card list. And next week a pair of you will score one for yourselves.
Get more info about our freebies on our Giveaway FAQs page. Pics courtesy of .


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Design and printing your marketing material can be a daunting task that's why we have put together a few categories under resources to help. Simply click any of the subsections under the resources at the top of page and that will take you to a world of knowledge.
If you can't find what your after or would just like to discuss anything in more detail please drop us a line on 01922 666141.


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1998 St. Hallett Faith Shiraz
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- Closure: Cork
A well made commercial wine. Superb deep crimson mauve colour. Appealing aroma of toasted oak, spice, vanilla, plum, liquorice and a final blackpepper end note. What starts off as very impressive is cut relatively short due to the lack of concentration of flavour on the palate. Spice, blackpepper, toasted oak and a touch of liquorice are carefully balanced by fine grained tannins. Plum, spice aftertaste. Cellar 3-5 years.


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What We Do
Looking is a brand and design consultancy based in Los Angeles. We work with an array of clients in diverse industries, enabling each client to articulate and express the unique qualities of their brand.
Our skills and experience have proved to clarify and create higher energy in a brand, to increase its impact through reasoned strategy and powerful design.
Who we are
John Clark
Principal, Designer
Czarah Castro
Marianne Thompson
Richard Kobayashi
Senior Designer
Extended Team
Ron Larson
Ron Larson Design Inc.
Tim Holmes
Reddoor Creative
Andy Engel
Andy Engel Design, Inc
James Robie
James Robie Design Associates
Where we are
145 Standard St
El Segundo, CA 90245
+1 310 322 6330


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June 27, 2016 was National Multiculturalism Day in Canada.
You'll be forgiven if you didn't know about it. Not that it means much, but I had no idea. I suspect the same is true for many Canadians.
Canada was the first country to adopt multiculturalism as an official policy, back in 1971. Doing so promotes the idea that all Canadians are equal. It affirms the value and dignity of everyone, regardless of racial or ethnic origin, language or religious affiliation. Multiculturalism helps encourage racial and ethnic harmony and cross-cultural understanding.
As an official policy, that's great. The reality on the ground is different. Most cultures tend to stick together. While this is natural, it means there are still barriers to achieving that cross-cultural understanding.
Here are some ideas on how actively become more multicultural.
- Become friends with at least two or three people who are from a different religion than you. Chances are they'll be from a different ethnic group than you as well. The twelve classical world religions (alphabetically) are: Baha'i, Buddhism, Christianity, Confucianism, Hinduism, Islam, Jainism, Judaism, Shinto, Sikhism, Taoism and Zoroastrianism.
- Choose to eat at small, ethnic restaurants. I like Wind, but it's not a true cultural experience in my opinion. Visit Kool Katts (Caribbean), Michinoku (Japanese), Spiice (Chinese), Duru (Korean)The Garden Restaurant (Greek), Passage to India (Indian / Pakistani), Afghan Horseman Kabob (Afghani), The Thai Dish (Thai), and any other non-chain restaurant that offers more authentic cooking. Learning about culture while experiencing new foods is great fun. If you're unsure what to order, ask the proprietors what they would recommend to someone who's never eaten that cuisine before.
- When you see a racial, religious or cultural stereotype, take some time to research whether it's actually true. It's easy for untruths and misinformation to be spread online. Take some time to educate yourself and others.


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Wow, what a great wedding last night at Barr Mansion! We started with cocktail music at 3PM, then dinner and dancing, finishing at 10PM. It was an awesome musical marathon!
Congratulations to Natalie & Andy on their big day. They gave me some ideas of what they wanted and really let me be creative and wow the people with music. Played lots of great classics and standards with some pop things through dinner and then rocked the dancers with some great rock-n-roll, country and 80s things.
It really feels great seeing people light up when they hear a song that delights them.


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The target blood sugar ranges for diabetic persons varies throughout the day and may range from 70 to 180 mg/dL, according to WebMD. Keeping tight control on blood sugar levels lowers a diabetic person's risk for several health issues including stroke, heart disease and kidney disease.
For non-pregnant adults over the age of 20, 100 mg/dL or less is ideal during fasting, and 70 to 130 mg/dL is optimal prior to meals, notes WebMD. After a meal, 180 mg/dL or less is normal. Before exercise -if insulin is taken- the target blood sugar level is at least 100, while before bedtime, 100 to 140 mg/dL is desirable.


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رومانسي جامد
سؤال البحث
نتائج البحث
بحث متقدم
قسم رسائل الجوال
150رساله بس انجليزي لهواة الانجليش من نار الحب وبس
كاتب الموضوع
عدد الرسائل
تاريخ التسجيل :
موضوع: 150رساله بس انجليزي لهواة الانجليش من نار الحب وبس
الجمعة يونيو 06, 2008 12:41 pm
1) Dark clouds hang over me sometimes...but I'll work it out.
2) The sparkle in your eyes could make the stars jealous.
3) Do you eat lucky charms? Because you look magically delicious!
4) Your friend is the man who knows all about you, and still likes you!
5) He has my heart…but everyday I get a little piece back...its time to move on...
6) Have you ever noticed that the worst way to miss someone is when they are right next to you and yet you know you can never have them.
7) Have you ever wanted to love someone with everything in you, but that other person was too afraid to let you?
Laundry is the only thing that should be separated by color!
9) You could die for Christ, but could you live for him?
10) Maybe God wants us to meet a few wrong people before meeting the right one, so that when we finally meet the right one, we know how to be grateful for that gift.
11) As long as there are tests, there will be prayer in public schools.
12) If the shoe fits, get another just like it.
13) One day you'll love me as I have loved you;
one day you'll cry for me as I have cried for you;
one day you'll want me…. and I won't want you!
14) God can mend all broken hearts; you just have to give him all the pieces.
15) Love is not a game, so why are there so many players?
16) Going to church does not make you a Christian any more than going to McDonalds makes you a hamburger!
17) Real friends are those who, when you feel you've made a fool of yourself, don't feel you've done a permanent job.
18) There are two things I've learned in life: 1) there is a God; 2) I am not Him!
19) Therapy is expensive, popping bubble wrap is cheap. You choose.
20) Don't cry cause its over; smile cause it happened.
21) I'm not inconsiderate, I just don't give a sh*t.
22) I'm sweet like sugar, soft like suede, but unlike Nintendo, I never get played!
23) Do I look like a grocery item? I see you checking' me out!
24) A kiss blown is a kiss wasted; the only real kind of kiss is a kiss tasted!
25) I'm loved by some, hated by many, envied by most, yet wanted by plenty!
26) God made coke, God made Pepsi, God made (name here) so darn sexy!
27) I wish I may, I wish I might, be the one you wish for tonight!
28) You can't fool a playa, but you can play them back!
29) God made rivers, God made dirt, God made boys so girls could flirt!
30) Everyone says you can only fall in love once, but that's not true, 'cause every time I hear your voice I fall in love all over again.
31) To the world you may be one person, but to one person you could mean the world!
32) If I could be an angel, I'd make your every wish come true, but I am only human, just a girl who's loving you!
33) It's not my fault I fell for you! You tripped me!
34) Every cutie with a bootie needs a hottie with a body!
35) I'm not mad at you, I'm mad at myself for thinking you're something you're not; and thinking that nothing would ever change, and you'd always be here for me.
36) I don't have an attitude problem, you have a perception problem!
37) It is impossible to rightly govern the world without God and the Bible.
38) Don't hate me cause I'm beautiful, hate me cause your man thinks I am!
39) Roses are red, violets are blue, give me all your kisses, and I'll surely repay you!
40) The greatest failure is not to try and fail, but to fail without trying!
41) You know you're in love when you don't want to go to sleep because reality is much better than a dream!
42) Roses are red, violets are blue, God made me perfect, what the heck happened to you!?!
43) No man is worth your tears and the one who is will never make you cry!
44) Eat to live, not live to eat!
45) Love isn't when you can be together and talk about anything, it's when you can be together and not say a word and think it's the best conversation you've ever had!
46) I was born to please and taught to tease!
47) If in my dreams is the only place I can hold you, I want to sleep forever!
48) Lost time is never found again!
49) Who do you go to when the one who usually stops your tears is the one who made you cry?
50) Should I smile 'cause you're my friend, or cry 'cause that's all you'll ever be?
51) Don't frown, you never know when someone might be falling in love with your smile!
52) Love isn't when you can imagine spending your life with someone; it's when you can't imagine your life without that person!
53) Star light; star bright, where the heck is Mr. Right???
54) Never say "I love you" if you don't really care; never talk about feelings if they aren't really there; never hold my hand if you're going to break my heart, never say you're going to if you don't plan to start. Never look into my eyes, if all you do is lie, never say "hello" if you really mean goodbye. If you really mean forever then say that you will try; never say forever, 'cause forever makes me cry!
55) There is something demoralizing about watching two people get more and more crazy about each other, especially when you are the only extra person in the room. –Silvia Plath
56) You were born in the heavens, up in the sky, sent down to earth to put a twinkle in my eyes.
57) Last night I matched up the stars to reasons I love you, I was doing fine, 'till I ran out of stars.
58) Good friends are hard to find, harder to leave, and impossible to forget!
59) Struttin' our stuff, shakin' our booties, me n my girls are pure cuties!
60) Don't hate me cause I'm beautiful, hate me cause…. uh…okay hate me cause I'm beautiful!
61) In order to love you have to risk the pain.
62) A peach is a peach, a plum is a plum. A kiss is not a kiss without some tongue!
63) An apple a day keeps the doctor away. But if the doctor is cute...screw the fruit!
64) Milk does the body good. But DANG...how much did you drink?
65) I only look sweet and innocent!!!
66) How do you do it...make me feel like I do?
67) Of all the friends I ever met, your the one I wont forget,
and if I die before you do, I'll go 2 heaven & wait 4 you!
68) I think I'm one of those bad things that happen to good people.
69) OH I'm sorry, did my back hurt you're knife?!
70) Real eyes Realize Real Lies.
71) Cross you're heart & hope to die, clothes & makeup, boys & lies, forever there 'till the end, Definition of: True Best Friends
72) Judge me if you want, but keep the verdict to yourself
73) You laugh because im different, I laugh because you're all the same
74) A Person Who Asks A Question Is A Fool For Five Minutes,
A Person Who Doesn't Is A Fool Forever ...
75) ReMeMbEr mY nAmE * ReMeMbEr My FaCe
* CuZ tHeRe AiNt nO oThA hOnEy * ThAt CaN tAkE mY pLaCe
76) I'm Loved by some, Hated by many, Envied by most, Yet wanted by plenty
77) everyones entitled to be stupid but YOU ARE ABUSING the priviledge!!!
78) One day your prince will come, mine just took a wrong turn, got lost, and is to stubborn to ask for directions
79) Don't call me a GODDESS, Don't call me a QUEEN, Just call me the cutest PRINCESS you've ever seen!
80) Boyz Are Great, Every Girl Should Have One
81) If I had the letters "HRT"
and I could add "EA" and get "HEART"
or add "U" and get "HURT",
I'd rather have "U" and get
"HURT" than have a "HEART" without "U".
81) You can fall from the sky,
you can fall from a tree,
but the best way to fall is in love with me.
82) When I saw you, I was afraid to meet you.
When I met you, I was afraid to hug you.
When I hugged you, I was afraid to love you.
Now I love you and I'm afraid to loose you. ~
83) To love someone is nothing, to be loved is something
84) Loved you then, love you still, always have, always will!
85) You never stop loving someone; you just learn to live without them.
86) If You Love Me... Let Me Know... If You Don't... Then Let Me Go...
87) Why cry over someone who wouldn't cry over you?
88) Never underestimate the power of stupid people in large groups
89) Suicide is a permanent solution to a Temporary problem.
90) They keep saying that the right person will come along; I think a truck hit mine
91) Don't say you love me unless you really mean it, because I might do something crazy like, believe it.
92) Heaven won't have me, and hells afraid I'll take over.
93) Did you fall down the ugly tree and hit every branch on your way down?
94) 4 out of 5 voices in my head say go back to sleep.
96) I know I'm not perfect, but I'm So Close it scares me
97) I like the way I feel, nothing hurts when no ones real.
98) I'm lost; please take me home with you.
99) A very wise man once said "I don't know go ask a woman".
100) I used to think I was indecisive, but now I'm not so sure
101) Maybe this world is another planet's Hell
102) If I were two-faced, would I be wearing this one?
103) I never forget a face, but I'll make an exception in your case
104) I quote others only in order to better understand myself.
105) If you love something set it free.
If it doesn't come back to you, chase it down and kill it
106) Guys are like roses, you gotta watch out for the pricks!
107) Loving you is like breathing, how can I stop?
108) Your boyfriend kisses better than mine.
109) Some people are alive just because it's against the law to kill them!
110) I wish I had the guts to walk away and forget what we have but I know you wont follow.
111) And your point is...?
112) You know you want me
113) If I were to throw a stick, would you leave?
114) I don't come with dice...so please don't try to play me
115) People like you are the reason we have middle fingers
116) I'm not a bad girl...just a good girl that does bad things..
117) Wish I had somebody, walking beside me, looking into my eyes at night.
I want a love to call my own.
I want someone that I can hold, someone wanting me.
Just want to know how it feels to be somebody's somebody
118) If you love something set it free..
.if it comes back, it was meant to be.
If it continues to fly, let it soar, have faith that God has something better in store.
119) There are certain people who are not meant to fit in your life,
no matter how much you want them to be
120) I don't miss him.... I just miss who I thought he was
121) It's really amazing when two strangers become the best of friends,
but it's really sad when the best of friends become two strangers
122) Sometimes it hurts more to smile in front of everyone than to cry all alone
123) It's funny the way you can get use to the tears & the pain
124) If you love your man, set him free..
If he doesn't come back, then you know he's with me
125) Sometimes its better to be alone, no one can hurt you that way
126) Sometimes when I'm alone at night I just sit and contemplate all the reasons why you wouldn't want me the way I want you
127) I may not see u as often as I'd like, I may not get to hold u all throughout the night,
but deep inside my heart I know this is true! No matter what, I'll never stop loving u!
128) I have never had a dream that has ever came true, until God gave me the gift of having u
129) You hurt me more than I deserve, how can you be so cruel?
I love you more than you deserve, why am I such a fool?
130) How can you simply be friends with someone when every time you look at them all you think about is how much more you really want them?
131) How do I tell him that he has my heart?
That without him my world would fall apart.
I need so much for him to know, but if I tell him will he stay or will he go?
132) Something in your eyes captured my soul, and every night I see you in my dreams. You're all I know. I can't let go.
133) Loneliness up ahead, emptiness behind, where do I go?
134) I know I made a lot of stupid mistakes in my life,
but the worst one was thinking the person who hurt me the most wouldn't hurt me again!
135) Waking up is the start of my nightmares.
136) I want to be remembered as the girl who always smiled...
The one who could brighten up your day, Even if she couldn't brighten her own.
137) The roots of love grow all around but for me they come tumbling down everyday heartaches grow a little stronger I cant take this pain much longer.
138) Have you ever loved someone and knew they didn't care?
Did you ever feel like crying but knew you'd get nowhere?
Do you ever close your eyes and say a little prayer?
Have you ever looked into his heart and wished that you were there?
139) There's a smile on my face but I don't know why its there...I put it on to try and satisfy all the people that don't even care.
140) Inside my heart is breaking but my smile stays on.
141) Say goodbye. Turn and walk away. You never really loved me-so why did I expect you to stay.
142) My face is always smiling but my soul wears an everlasting frown.
143) Funny how we feel so much but cannot say a word,
we are screaming inside but we can't be heard.
144) What can you say about a society that thinks God is dead and Elvis is alive?
145) Screw Snapple I'm the Best stuff on Earth!!!
146) Coffee, chocolate, men...some things are just better rich!
147) What a shame...looks like the ugly fairy kissed you on both cheeks!
148) Don't look at me like I'm evil.
149) If I could give you one gift in the world it would be the ability to see yourself as I see you.
Maybe then you'll realize how special you really are!
150) I would tell you to go to hell, but all dogs go to Heaven!
151) A smile is a curve
[b]>>> مـن جن بالحب فهو عاقل , من جن بغيره فهو مجنون , وليس من الصعب أن تضحى من أجل الحبيب , ولكن من الصعب أن تجد الحبيب الذى يستحق التضحية
150رساله بس انجليزي لهواة الانجليش من نار الحب وبس
من اصل
صلاحيات هذا المنتدى:
الرد على المواضيع في هذا المنتدى
قسم رسائل الجوال
قسم رسائل الجوال
انتقل الى:
اختر منتدى
|--المنتدى العام
|--منتدى الترحيب بالاعضاء الجدد
|--المنتدي السياسي
|--المنتدى الأدبي
|--منتدي المعلومات العامه
|--منتدي العلوم النفسيه
|--منتدى الرجل
|--قسم الاعمال المنزليه
|--قسم المطبخ ووصفات الاكل
|--قسم الازياء والموضه
|--منتدى المرأة
|--منتدى الالبومات الكامله
|--منتدي el_____gazar_____d$j
|--اغاني الرااااب
|--قسم الالبومات الاجنبيه الكامله
|--منتدي الاغاني الاجنبيه
|--منتدي الفيديو الكليب الاجنبي
|--الفيديو كلييب العربي
|--منتدي اخبار الفن والفنانين
|--منتدي الاغاني والفديو كليب
|--كلام في الحب وعن الحب
|--منتدي القصص الرومانسيه
|--منتدى الصور الرومنسيه
|--المشكلات العاطفيه والرومانسيه
|--المشكلات العاطفيه والرومانسيه
|--منتدي الشعر
|--واقع الحياه
|--كرسي الاعتراف
|--صوتيات وفديوهات وبرامج اسلاميه
|--الاناشيد الاسلاميه
|--السنه والاحاديث النبويه الشريفه
|--المنتدي الاسلامي العااااااااااااام
|--قسم الجن ولاشباح
|--غرائب وعجائب العالم في صور
|--ركن القصص ولاساطير
|--منتدى الجن والغرائب وما وراء الطبيعه
|--عمرو دياب
|--كلمات الاغاني
|--جورج وسوف
|--كلمات الاغاني
|--سامو زين
|--كلمات الاغاني
|--مصطفي قمر
|--كلمات الاغاني
|--محمد فؤاد
|--كلمات الاغاني
|--كلمات الاغاني
|--كاظم الساهر
|--كلمات الاغاني
|--قسم المالتيميديا والجرافيكس الخاصة بالنادي الأهلي
|--اساطير أهلاوية
|-- رابطه مشجعي النادي الزمالك
|--قسم المالتيميديا والجرافيكس الخاصة بالنادي الزمالك
|--اساطير الزمالك
|--منتدي الدورى العالمى
|--منتدي الكورة الايطاليه (الكاتشيبو)
|--منتدي الكورة الانجليزيه(البربميرليج)
|--منتدي الكورة الاسبانيه (الليجا)
|--منتدي اجمد مصارع
|--منتدى الصور العام
|--الصور الدينيه
|--منتدي صور الفنانين
|--صور وخلفيات
|--بوسترات الألبومات
|--منتدي الافلام العربيه
|--منتدي المسرحيات العربي
|--منتدي المسلسلات العربيه
|--افلام انجليش اون لين وانت قاعد ع النيت
|--منتدي الالعاب الجماعيه
|--تحميل الالعاب بروابط مباشره
|--قسم مشاكل وحول الالعاب والكراك
|--منتدي الالعاب
|--منتدي الالعاب اون لين
|--برامج الحمايه
|--قسم برامج المدبا والصوتيات
|--منتدى البرامج العام
|--قسم برامج الخطوط
|--قسم الفوازير والتسالي
|--قسم الالعاب والتسالي
|--منتدى الموبيل العام
|--قسم الفديوهات ومفاطع البلوتوث
|--قسم النغمات
|--برامج الموبيلات
|--ألعاب الموبيل
|--قسم رسائل الجوال
|--قسم شكاوي الاعضاء
|--قسم الاستفسارات والاسئله
|--قسم المواضيع المحذوفه
|--قسم الصور الدينيه
|--منتدي الالحان والترانيم
الحصول على منتدى مجاني
منتدى مجاني للدعم و المساعدة
التبليغ عن محتوى مخالف
انشئ مدونتك الخاصة


text:
I was just reading in the papers about the decision of Wal-Mart dropping HD DVD for Blu-ray, and wondering what the impact of that would be. I guess I found out. Toshiba has just officially announced the discontinuation of its high-definition video format, HD DVD, after numerous bigwigs such as Wal-Mart, Netflix, Best Buy and many others abandoned HD DVD for Sony's Blu-ray.
It appears that Wal-Mart was the final straw. With no major players backing up its format, Toshiba could only admit defeat. HD DVD was the first to appear on the market, and is cheaper by a margin, but it appears that Blu-ray wins out. It's not a bad thing, because Blu-ray is technologically more superior. In any case, it's still a fact that is made even clearer after this episode that the majority of consumers will create enormous market forces that can make or break a new technology or format.


text:
Signs that vehicles have been damaged in floods include: water marks or lines on exterior surfaces, such as the doors and wheel wells, water marks on interior surfaces and the glove compartment and rusting or corroding parts. Checking for water damage before buying a car is essential, say experts at DMV.org as owners can dry out and clean cars to make them appear dry despite having heavy water damage. Furthermore, damage from floods can take several years to fully manifest, which leaves unsuspecting owners with the burden of repairs.
For car problems facing shoppers, purchasing water-damaged cars is one of the largest problems, say authors at the DMV. In addition to cleaning and drying the interior, car sellers can assign new license plates, which gives consumers a false sense of security.
Before buying a car, the DMV urges people to perform a background check on the vehicle. This involves running a vehicle history report on the car, using the vehicle identification number as a reference. Prospective owners can perform a visual check of outside and interior surfaces to look for mud and silt. The engine, trunk and headlights should also be free of water lines, dirt and silt. Those new to car shopping can also hire a third-party mechanic to perform inspections. Lastly, shoppers should be wary of sellers who hesitate to show certain parts of the car, such as engine and trunk.


text: Coordinator's Column | July 01, 2009From the Coordinator Lynne E. Hewitt Author Notes © 2009 American Speech-Language-Hearing Association Article Information Coordinator's Column Coordinator's Column | July 01, 2009 From the Coordinator SIG 1 Perspectives on Language Learning and Education, July 2009, Vol. 16, 39-41. doi:10.1044/lle16.2.39 SIG 1 Perspectives on Language Learning and Education, July 2009, Vol. 16, 39-41. doi:10.1044/lle16.2.39 View Article Figures Tables PDF PDF Supplemental Data Supplements Multimedia Share Email Twitter Facebook Pinterest Tools Get Citation Citation Hewitt, L. E. (2009). From the Coordinator. Perspect Lang Learn Educ, 16(2), 39-41. doi: 10.1044/lle16.2.39. Download citation file: RIS (Zotero) EndNote BibTex Medlars ProCite RefWorks Reference Manager © 2018 American Speech-Language-Hearing Association × Alerts User Alerts You are adding an alert for: From the Coordinator You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account The alert will be sent to: Confirm × Sign In or Create a free account to receive alerts. × Division 1 continues its tradition of bringing you opportunities for professional learning and high quality CE offerings. For this year's ASHA Convention, we are focusing on early intervention; Luigi Girolametto and Elaine Weitzman of the Hanen Center in Toronto will be presenting our Pre-Convention Clinical Workshop, on Wednesday November 18th, from 1:00 to 5:30 pm. This is offered for .4 CEUs. I hope those of you in the New Orleans area or those coming to the ASHA Convention can take time to attend. The Hanen program is long established and evidence-based. It emphasizes a parent training model of early language stimulation for children with developmental disorders and language impairments. The title is "Parent Programs That Work! The Efficacy of Parent Training for Preschoolers With Language Disorders." Access the agenda and registration form online at http://www.asha.org/members/divs/div_1.htm Note: Division 1 affiliates get a steep discount on registration fees, at least equal to the cost of your annual affiliation dues. Subscribe to view more For full access to this article, log in to an existing user account, become a SIG affiliate, or purchase a short-term subscription. Become a SIG Affiliate Join a SIG Pay Per View Entire SIG 1 Perspectives on Language Learning and Education content & archive 24-hour access $25.00 Buy Now This Issue 24-hour access $17.00 Buy Now This Article 24-hour access $10.00 Buy Now Sign In or Create an Account Please sign in using your ASHA.org login. If you do not have an ASHA login, you may register with us for free by creating a new account. Sign In or Create an Account We've Changed Our Publication Model... The 19 individual SIG Perspectives publications have been relaunched as the new, all-in-one Perspectives of the ASHA Special Interest Groups. Learn more > Related Articles Related Topics


text:
Hotels in Dumbria, Spain
Search & Compare Dumbria Hotels
Save more with Secret Prices
Get instant savings with Secret Prices
Best hotels in Dumbria
Dumbria overviewWhatever your interests are, Dumbria has something for you. Visitors to Dumbria can take time to see places like Mirador de Ézaro and Ezaro Beach. You won't want to miss sights in the surrounding area like Ezaro Waterfall and Molinos de San Adrián de Toba.
Where to stay in DumbriaA welcoming city for everyone, Dumbria has a variety of hotels and other lodging options. There are 7 hotels in Dumbria and you can shop as many as 21 deals for rooms on Hotels.com, some with discounts of up to 10%. Our room prices start at 15 USD per night and here you'll find a breakdown of hotels by star rating in Dumbria as well as in the surrounding area:
- • 57 4-star hotels from 56 USD per night
- • 53 3-star hotels from 52 USD per night
- • 14 2-star hotels from 21 USD per night
What to see around DumbriaRecommended sights include:
- • Mirador de Ézaro
- • Ezaro Beach
When to visit DumbriaDo you want to know the ideal time of year to visit? Here's a list of the seasonal weather norms to help with trip planning:
Seasonal temperature averages
- • January-March: 63°F day, 41°F night
- • April-June: 75°F day, 43°F night
- • July-September: 78°F day, 52°F night
- • October-December: 68°F day, 42°F night
- • January-March: 0.35 inches
- • April-June: 0.35 inches
- • July-September: 0.24 inches
- • October-December: 0.39 inches


text:
Win a pair of Cricket Chairs from Karkula!
Name: A pair of Cricket Chairs by L'Abatte in Black
Shop: Karkula
Value: $560
Today in our Holiday Giveaway we're pleased to present a set of two Cricket Chairs by L'Abatte in Black from Karkula. Designed and crafted in Italy, L'Abatte specializes in lightweight yet well-crafted seating ideally suited for small spaces. These chairs have a painted seat and back, chrome legs and a circular hole cut in the seat back. They're practical and stylish. To enter this Giveaway, please click here. To see all our Giveaways, click here.
Please fill out the submission form in this post. You are allowed ONLY ONE ENTRY PER PERSON PER GIVEAWAY ITEM. Duplicate entries will be discarded. Entries for each posted item are open for 3 days from the date of posting (for example, if a design gift is posted at 12:00 pm EST on Monday, it will be open for entries until Thursday at 12:00 pm EST). Entries generated by script, macro or other automated means are void. Normal time, toll, connection and usage rates, if any, charged by your Internet service provider will apply. All entries become the property of the Apartment Therapy Media. Winners will be selected at random. All registrations must include entrant's valid email address. The email address provided by online entrants will be the identity of the entrant and, if selected for a prize, the identity of the winner. All prizes will be shipped to the address listed on your entry form. You must be a U.S. resident, age 18 or older to enter. Employees, partners and vendors of Apartment Therapy and Martha Stewart Living Omnimedia and their immediate family members are not eligible to enter. For complete rules, please click here.