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Microarrays 2015, 4(2), 188-195; https://doi.org/10.3390/microarrays4020188
Tissue Microarray Technology for Molecular Applications: Investigation of Cross-Contamination between Tissue Samples Obtained from the Same Punching Device
Translational Research Unit (TRU), Institute of Pathology, University of Bern, Murtenstrasse 31, Room L313, CH-3010 Bern, Switzerland
Author to whom correspondence should be addressed.
Academic Editor: Luigi Terracciano
Received: 5 January 2015 / Accepted: 26 March 2015 / Published: 2 April 2015
Background: Tissue microarray (TMA) technology allows rapid visualization of molecular markers by immunohistochemistry and in situ hybridization. In addition, TMA instrumentation has the potential to assist in other applications: punches taken from donor blocks can be placed directly into tubes and used for nucleic acid analysis by PCR approaches. However, the question of possible cross-contamination between samples punched with the same device has frequently been raised but never addressed. Methods: Two experiments were performed. (1) A block from mycobacterium tuberculosis (TB) positivetissue and a second from an uninfected patient were aligned side-by-side in an automated tissue microarrayer. Four 0.6 mm punches were cored from each sample and placed inside their corresponding tube. Between coring of each donor block, a mechanical cleaning step was performed by insertion of the puncher into a paraffin block. This sequence of coring and cleaning was repeated three times, alternating between positive and negative blocks. A fragment from the 6110 insertion sequence specific for mycobacterium tuberculosis was analyzed; (2) Four 0.6 mm punches were cored from three KRAS mutated colorectal cancer blocks, alternating with three different wild-type tissues using the same TMA instrument (sequence of coring: G12D, WT, G12V, WT, G13D and WT). Mechanical cleaning of the device between each donor block was made. Mutation analysis by pyrosequencing was carried out. This sequence of coring was repeated manually without any cleaning step between blocks. Results/Discussion: In both analyses, all alternating samples showed the expected result (samples 1, 3 and 5: positive or mutated, samples 2, 4 and 6: negative or wild-type). Similar results were obtained without cleaning step. These findings suggest that no cross-contamination of tissue samples occurs when donor blocks are punched using the same device, however a cleaning step is nonetheless recommended. Our result supports the use of TMA technology as an accessory to PCR applications.
Keywords:tissue microarray; biomarker; digital pathology
1. Introduction
Tissue microarrays (TMAs) play an important role in translational and clinical studies [1]. By concentrating hundreds of small tissue cores typically of 0.6 mm in diameter onto a single paraffin block, dozens of biomarkers can be studied on a large number of patients while at the same time sparing costs, resources and tissues [2,3,4]. TMAs are used to study protein markers by immunohistochemistry and to investigate DNA aberrations, mRNA and miRNA expression using various in situ hybridization techniques [5,6,7]. Together with clinically annotated and appropriately powered sample cohorts, TMAs are a powerful tool not only for biomarker screening but also for prognostic and predictive modeling of disease outcome. In addition, TMAs allow that the same experimental conditions be applied to hundreds of samples simultaneously [8].
Recent advances in TMA technology rely on digital pathology and automated tissue microarraying. One approach is to use a slide scanner to digitally visualize stained tissue sections, which can then be annotated using a TMA tool of various sizes (Figure 1). These annotated slides are matched to their corresponding donor blocks, which are precisely punched out at the desired locations and transferred into recipient blocks, automatically. At our institute, this approach of combining modern TMA technology with histopathological expertise and biostatistics is referred to as next-generation TMA (ngTMA) [9].
Figure 1. (A) Digital annotation of a scanned slide after double-immunohistochemistry staining with pan-cytokeratin and caldesmon; (B) Hematoxylin and Eosin staining of a tissue microarray (TMA) constructed from annotated regions of histological interest.
Clearly, TMA instrumentation could also be advantageous for applications other than construction of TMAs. In fact, punches taken from donor blocks can be placed directly into tubes and therefore used for molecular analysis using PCR-based approaches.
However, the question of possible cross-contamination across tissue samples using the same punching device, especially one embedded in an automated tissue microarrayer, has frequently been posed but never addressed. This may be of particular concern if nucleic acids are amplified by PCR. Therefore, in this study, we determine the level of contamination transferred between samples after automated punching using a sensitive assay for mycobacteria and a pyrosequencing assay for KRAS mutation analysis.
2. Experimental Design
2.1. Patients
Mycobacteria assay: In a first step, we identified two patients recently diagnosed at the Institute of Pathology, University of Bern. The first was a 21 year-old male with a histological diagnosis of granulomatous lymphadenitis compatible with tuberculosis. A molecular test for mycobacterium tuberculosis, bovis and BCG was made and confirmed the presence of the bacteria. The second was a 67 year-old woman with right-sided colon cancer and no evidence of mycobacterial infection. Formalin-fixed paraffin-embedded tissue blocks from the lymph nodes and cancer, respectively, were retrieved from the tissue archive.
KRAS assay: In a second step, we identified six patients also recently diagnosed with metastasizing colorectal cancer diagnosed at the same Institute. All underwent mutation analysis for KRAS Exon 2, codons 12 and 13 using pyrosequencing. Three patients were wild-type (WT); one patient had a colorectal cancer with G12D mutation, the second a G12V mutation and the third was mutated in codon 13 (G13D).
2.2. Analysis of Alternating Mycobacterium Positive and Negative Tissue Samples
First the mycobacterium-positive and then negative tissue blocks were loaded into an automated tissue microarrayer (TMA Grandmaster, 3D Histech, Budapest, Hungary). Four punches at 0.6 mm in diameter were taken from the positive sample and transferred to a 0.2 mL PCR tube. The punching tool was mechanically cleaned in an empty paraffin block by insertion and removal multiple times (Figure 2). Then, the negative tissue block was cored similarly and tissue punches placed into the respective tube. Again a mechanical cleaning took place. This sequence was repeated three times such that six different tubes containing alternating positive and negative samples were obtained.
DNA was isolated from tissue punches by overnight digestion with proteinase K following purification using a BioRobot EZ1 (Qiagen, Hilden, Germany). The primer pair used for PCR amplification of a fragment from the 6110 insertion sequence specific for mycobaterium tuberculosis complex was 5'-CCTGCGAGCGTAGGCGTCGG-3' and 5'-GTTTCTCGTCCAGCGCCGCTTCGG-3'. The forward primer was labeled by FAM. Amplification was performed in 40 cycles of 95 °C for 1 min, 62 °C for 1 min and 72 °C for 1.5 min. The PCR product was analyzed by capillary electrophoresis using a Genetic Analyzer 3500 (Life Technologies, Zug, Switzerland). 5 fg of mycobacterium tuberculosis DNA corresponding to 5–10 bacteria was used as a positive control. Human DNA was used as a negative control.
Figure 2. Schematic diagram showing (above) the alternating sequence of mycobacterium-positive and negative tissue blocks in the arrayer; (below) alternating colorectal cancer tissue blocks with known mutational status, followed by mechanical cleaning of the punching device in a paraffin block.
2.3. Analysis of Alternating KRAS Mutated and Wild-Type Tissue Samples
In order to compare the level of contamination between a manual punching device without any cleaning step between donor blocks and the automated device with mechanical cleaning step described above, KRAS analysis was performed. Using a punching device from a homemade semi-automated tissue microarraying instrument, four punches of 0.6 mm in diameter were cored out from the tumor blocks in the following sequence: G12D, WT, G12V, WT, G13D, and WT. Punches were placed into separate tubes for further analysis. Next, the same tissue blocks were loaded into the automated tissue microarrayer and punched out using a 0.6 mm tool × 4 times according to the same sequence, as above. Between each block, a mechanical cleaning step was performed by insertion of the device into an empty paraffin block several times.
DNA was extracted using standard protocols (QIAamp® DNA FFPE Tissue, Qiagen). PCR was performed using the Pyromark PCR kit (Qiagen) with the following primer sequences for KRAS (Microsynth®, Balgach, Switzerland): Forward 5'-TAA GGC CTG CTG AAA ATG ACT G-3', Reverse 5'-TTA GCT GTA TCG TCA AGG CAC TCT-3' and Sequencing 5'-CTT GTG GTA GTT GGA GC‑3'. The PCR conditions were as follows: activation step at 95 °C for 15 min, denaturation 30 s at 94 °C, annealing 35 cycles of 30 s at 60 °C, and extension 30 s at 72 °C and final extension 10 min at 72 °C. After PCR, fragment analysis was carried out using a Qiaxcel system (Qiagen). Then, the mutation analysis of KRAS (exon 2, codon 12 and 13) was performed using the pyrosequencing method using a PyroMark Q24 (Qiagen). The sequence to analyze was TGNTGRCGTAGGCAAGAGT GCCTTGACGATA. In addition, a control oligo and water control were used in the pyrosequencing as well as appropriate positive and negative of KRAS mutation controls.
3. Results/Discussion
3.1. An Alternate Use of TMA Technology
TMA technology combined with digital pathology can help strengthen biomarker research [10]. Recent advances allow precise histological areas to be digitally annotated onto scanned tissue slides. These annotations are then used to identify specific regions from the corresponding tissue block that should be punched out and transferred to the TMA. Another possible application of this technology is to punch out and transfer tissues directly into tubes, which can then be used for subsequent molecular analysis. However, the possibility for cross-contamination between samples has not yet been addressed. This contamination could arise from tiny amounts of material remaining on the punching device that can be transferred from one tissue sample to the next.
3.2. Cross-Contamination Assessed Using Mycobacterium Tuberculosis Assay
To assess cross-contamination between punches, we alternate mycobacterium tuberculosis-positive and negative tissue blocks in an automated tissue arrayer with a mechanical cleaning step in between. Tissue blocks were punched out sequentially. The presence of mycobacterial DNA in the tissue punches was analyzed by PCR using a primer pair specific for IS6110, an element, which is present at multiple copies in the genome of mycobacterium tuberculosis complex [11]. This method is proven to be highly sensitive as it allows for the detection of as little as 1–2 mycobacteria. A positive mycobacterium result would be indicated by a peak at 123 bp in the corresponding electropherogram. A fragment of 123 bp was clearly detected in the DNA from punches from mycobacterium tuberculosis-positive tissues (Figure 3A,C,E). In contrast, mycobacterium-negative tissues punched out in between, were found to be negative (Figure 3B,D,F). Thus, there appears to be no transfer of mycobacterium tuberculosis from the positive to the negative case.
3.3. Cross-Contamination Assessed Using KRAS Analysis by Pyrosequencing
Since the detection of mycobacterium in the negative samples may be influenced by the level of infection, we carried out the analysis of KRAS mutational status on colorectal cancers. Using only a manual punching device without cleaning step and alternating between tissue samples, no cross‑contamination could be seen. When analysis of KRAS gene mutation was performed using the automated device with cleaning step, again no cross-contamination of tissue samples was found. All mutated samples carried the same mutations, while all WT samples were clearly WT (Figure 4).
3.4. Impact of the Finding
Researchers working with modern TMA technologies have a growing interest in the possible application of TMA instrumentation for purposes other than TMA block construction. Especially, a combined digital pathology/TMA approach is ideal for assessing issues of heterogeneity and allows very precisely annotated histological regions to be transferred either to TMAs or to tubes for storage and/or analysis of nucleic acids. With or without a digital pathology platform, neighboring punches side-by-side for TMA construction and simultaneous molecular analysis is clearly advantageous in the era of molecular pathology.
This study was motivated by the growing concerns over cross-contamination between samples and appears to be the first study to date to address this issue. Using two different PCR methods and alternating between positive/mutated and negative/wild-type samples, our findings underline no evidence of transfer of material from one tissue block to the next.
Figure 3. Electropherograms used to evaluate the presence of mycobacterium in donor blocks from a previously confirmed positive and negative tissue sample. (A) positive control and (B) negative control. Tissue samples were alternated in the tissue arrayer three times leading to the following results: (C,E,G) mycobacterium tuberculosis samples and (D,F,H) mycobacterium tuberculosis negative samples. No evidence for cross-contamination between samples.
3.5. Conclusion
To conclude, our finding suggests that TMA instrumentation is appropriate for use as an accessory to molecular applications. No cross-contamination appears to occur between samples punched with the same device, although a cleaning step in between donor blocks is still recommended.
Figure 4. Pyrograms highlighting results from an analysis of KRAS exon 2 codon 12 and 13. (A) G12D mutation; (B) WT; (C) G12V mutation; (D) WT; (E) G13D mutation; (F) WT.
Author Contributions
All authors were involved in study design and conception. EV and JAG performed experimental analyses. IZ drafted the manuscript. All authors approved the final version.
Conflicts of Interest
The authors declare no conflict of interest.
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'Spin Your Wheels' along the Aspinwall riverfront April 6
No matter the weather on April 6, the Spin Your Wheels fundraiser at Aspinwall Riverfront Park will see the shoreline abuzz with activity.
From spinning class to yoga and boxing, an array of exercise classes will be offered for all ages from 9 a.m. to 4 p.m.
Cost begins at $25.
Proceeds benefit Riding for the Handicapped of Western PA (RHWPA), a group that works to advance the progress of disabled children through therapeutic horseback riding.
With the event now in its fifth year, organizers promise to outdo every previous effort, co-chairs Jennifer Evashavik and Katherine Hook said.
"It's like a big party. We try to make it better each year," Evashavik said.
Aspinwall Riverfront Park, with the glistening Allegheny River and marina, will host four spinning classes, Zumba, yoga, high intensity interval training, Power Fusion, boxing tutorials and other sessions scheduled in the large, open-air space by the docks.
There will be 14 spinning bikes, borrowed from Tribe 412 Fitness on Fox Chapel Road, to pedal on the porch beside the river. An awning overhead will protect participants, even if it snows like last year.
Sign-ups for all sessions are online at spinyourwheels.org.
The college friends from Wittenberg University started the fundraiser for autistic youngsters and others with disabilities. Evashavik's son has been riding with RHWPA for a decade, and both women have seen firsthand the joy when a child sits high on a horse.
And the animals from a McCandless stable are retired equines continuing useful lives.
This year's theme is, "Become an exercise hero for yourself and the kids."
Even though the spinning bikes gave the fundraiser its name, there is something for everyone and every age.
There's kid's boot camp with 30-minute sessions. A trail around the Allegheny riverfront and boardwalk provide an adventure.
Also offered will be Mindful Movement, a free class adapted for every child in the room off the patio with its row of French doors.
Exercise classes are the center of Spin Your Wheels, but the event has many other spokes.
New this year is graffiti-pop artist Luca Paganico, who will create performance art. Free coffee courtesy of Beans 'n' Cream and other beverages such as Bloody Marys will fuel the day.
Specialty drinks will be sold from Main Squeeze Juice truck.
And when the kids aren't exercising, childcare is free.
Many local businesses and residents have donated items for raffle. Last year, a whole wall was set up and this year will be even better, Evashavik said.
Some vendors will be on hand and donating a portion of their sales.
Each participant gets a gift bag and a T-shirt.
"You can't believe it until you see it," Hook, a teacher at Hartwood Elementary, said.
Freebies include Motive and Bloom salons' braid bars, Michelle Trumble's mini-manicures, Klavon's ice cream, Maida Steel's chair massages and other prizes all spread out along Aspinwall's riverfront.
The fundraiser is a chance to reinforce community feeling, too.
Evashavik, her husband Nick, and sons Luke and Ben; and Hook, husband John, and teens Emily and William, are Aspinwall residents.
Hook said the fundraiser "pulls the community in. Aspinwall is a strong-knit community."

subset: commoncrawl


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So, you have a cycle, you want to take it out, but don't know where? Don't fret. We give you the skinny on much-loved cycle routes in and around town, from city centre, to heading well and truly out of town. In fact, we'll go a step ahead and even hook you up to these routes on Strava – a much loved app for cyclists, and the Facebook equivalent for cardio-lovers. Go out and cycle, then upload your ride stats, get kudos from others following you and track progress over time and feel justifiably proud of yourself. Note, though, the best time to head out cycling has to be the morning, while most of the city is asleep. Ready? Let's roll.
Hop On Your Bicycle And Take The Routes Less Travelled
Ulsoor Lake
This pretty little 3.3km route is a great option if you're starting out. It gives you a good chance to get used to your bike and navigating traffic. It also helps keep speed in check and learn to practice consideration for others in traffic.
Difficulty Level: 1/5
Hennur Bamboo Forest
If off-roading is your thing, you'll love this place. There's gorgeous trails, some easy to traverse and some not. Located just about 15 minutes away from the Outer Ring Road junction this forest offers plenty of quiet to be enjoyed, birdsong to be listened to, and greenery to be taken in. Start in the region of Kingston County, an up-and- coming gated community and just go exploring.
Difficulty Level: 2.5/5
Ride To Nandi Hills & Climb
The one thing that Bangalore is not, is flat. The rolling roads beyond the airport turnoff lead you to Nandi Hills. This oft-cloud-covered challenge can be summited after an 8km tough ride. Not for the faint of heart, especially given the amount of vehicular traffic. But if you're willing to give it a shot once, be warned, this has the ability to become a monthly affair!
Difficulty Level: 5/5
Tavarekere-Sondikoppa Road
The least boring route in the vicinity of Bangalore – no single straight flat stretch of road extending beyond a kilometer, this one is exciting. Multiple thriving lakes, peacock-filled forests and calm villages awaits the rider on this undiscovered route to the Tumkur highway from South Bangalore.
Difficulty Level: 3/5
The Big Big-Banyan- Tree Loop
The Big Banyan Tree has been the finish line of many a 'My first 25km bicycle ride' and 'My first 50km bicycle ride'. Once you escape the traffic of Mysore road, you are transported back in time to a place that still holds the essence of 'Pensioner's Paradise'. A little known deviation is to take a right after the Big Banyan Tree and head towards Tavarekere. Beware though, the short steep climbs will test your mettle.
Difficulty Level: 4/5
The Love Triangle
Looking for a day long trip to escape from the hustle of the city, then this is the perfect route for you. Make sure to leave early to avoid the traffic on the zippy Tumkur Highway. As soon as you reach Nelamangala, the wide NH4 gives you space a plenty to ride stress-free. The left turn to heaven after Kunigal leaves you never wanting to go back home! Minimal traffic (perhaps one tractor passes by once every 30 minutes), water-lily filled lakes and smooth tarmac will make you want to thank us. Once you reach the Mysore road, despite the slightly large traffic, the mainly downhill nature of the stretch will have you back in the Ooru in no time.
Difficulty Level: 5/5
Old Madras Road
The national highway to the erstwhile gold mine won't have you gaping at the scenery, but if you are looking at a fast-paced training ride, this is the stretch to be on. The strong headwind on the way back will have you cursing yourself for the decision to ride here but the post-ride satisfaction of overcoming the obstacle washes it away.
Difficulty Level: 3.5/5


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My name is Nengi Willie-Pepple and I love material things.
Not to say that I don't love non-material things that is, in fact I love non-material things a lot more. However, my love for material things is pretty damn strong.
Lets rewind back to the year 2001. If my calculations are right, I was eight years old then. I loved material things so much that I would carry the shopping bag out of the store, refusing to let anyone assist me. Regardless of how heavy it was, it was mine and no one (even the person who paid for it – my mum) was touching my brand new things.
When I got home I would take it out of the wrapping and admire it for hours. Be it new school shoes, a new dress, Barbie etc. But let's solely make clothing the topic of discussion here.
So, after admiring said item(s) I would fold them neatly and place them in a special section of my wardrobe. This was my thing for years and years and truthfully I was happy with this behaviour. …until I wasn't.
With this behaviour of keeping my new purchases neatly tucked away, what happened was that, by the time I deemed an event special enough to wear these 'too good for just ANY event' items of clothing, the clothes or shoes were too small for me. I had to be stopped.
Fast forward to 2009, my mum made me realise that this was such a silly habit. "You're a growing child Nengi, it just doesn't make sense! I know you'd prefer to save them, but time waits for no one!" With that new piece of information, I slowly broke the habit.
All this back-story is to let you in on how the Nengi of 2001 almost crept back into 2016, as I wanted to save this Balmain/H&M number for my birthday. Let's not forget, the collaboration dropped in November 2015.
Your girl actually adored the dress so much that she considered the 5-month wait until her birthday. As you can see by the pictures, this did not happen and I wore it out when I was in Nigeria. Although my body hasn't changed much in the past 5 years, (no longer a growing child), time doesn't wait for anyone and when you think about it, there will always be amazing clothes and shoes out there. Fast fashion is a never-ending cycle.
Here is a link to how coveted a piece from the collection was. Thankfully I didn't experience the craziness on the streets of London, as I purchased mine online.
BalmainxH&M Dress (sold out), Missguided Heels


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For more information see the Copyright and Permission FAQ.
A black ink on long fiber board paper woodcut of four geese with water in the foreground, by Herschel Logan. One of the Prairie Printmakers, Logan executed this work in 1927, and said this was "from a sketch done on the old boyhood farm near Winfield." Logan was born April 19, 1901 in Magnolia, Missouri, and the family moved to Winfield, Kansas shortly afterwards. He attended the Chicago Academy of Fine Arts. He worked as an advertising artist in Salina until his retirement in 1968. He moved to Santa Ana, California, where he died December 8, 1987.
Creator: Herschel C. Logan
Date: 1927
Item Number: 222132
KSHS Identifier: 1977.170.49
Business and Industry - Occupations/Professions - Artists
Collections - Museum
Community Life - Arts and Entertainment - Art - Printmaking - Prairie Print Makers
Date - 1920s - 1927
Environment - Wildlife - Birds - Geese
Objects and Artifacts - Communication Artifacts - Original Art - Picture - Nature
People - Notable Kansans - Logan, Herschel C., 1901-1987
Places - Cities and towns - Winfield
Places - Counties - Cowley
Type of Material - Objects and Artifacts


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When school wraps up for another year, the learning can go on! Help keep your son or daughter motivated and excited with fun-filled summer camps in Worcester from Sylvan Learning.
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DNG (Dedicated 'N Galvanic), is a rapper & producer from The Hague City (Netherlands). Inspired by various US rap artists at the beginning of the so called Golden Era of Hip Hop, DNG started rapping and making beats himself at the age of 14. After being part of several short lived rap crews in the local underground rap scene DNG released his first demo tape ("Gattin' Down") in 1999. This was also the year when he teamed up with three other gifted and hungry mc's from the Lake Side Area to form the rap group Coup deVillz. Being part of Coup deVillz - with whom he rocked many stages – however didn't stop DNG from also working on solo material. In 2006 he released the "MiniMeMixtape" and his first animated video "Lil Nut". With two independent albums under their belt the rap group eventually split up in 2009.
start rapping
years age
first demo
'gattin' down
and more…
Still passionate about the creative aspect of writing and producing, DNG prepared himself for another independent project and finally released his debut album Regardless in 2011 on his label Bloodstream Music. The album featured guest appearances from his former partners in rhyme Slowgo and Cavy G as well as female vocalist Imen and horn player Michael Varekamp and was also complimented with 5 videos directed by Vin Rock. Even though it has been quiet after the release of his debut album, DNG has been working on new material. "I know it's quite tempting in this era of social media to blog about every step in the creative process just to keep your name ringing but I prefer to stay low key. Once I publicly announce something regarding my music, it'll be about finished product." In case one wonders if the rapper will release another album or EP, DNG has decided not to bring out his new music in aforementioned formats "Based on my personal situation, I'd rather drop singles instead of another album."
As far as his goals for the future, DNG keeps it simple and plain.
"The meaning of the first letter in my name as well as my label imprint are self-explanatory. I've been doing this for quite some time and I'll probably never stop making music. For now, I'll just have fun with it, throw some new music out there and see where it will take me."
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Thursday, September 27, 2012
My Booties obsession and a $100 Shopbop Gift Card Giveaway!
91 comments:
Such a great giveaway!ReplyDelete
Peyton, firstname.lastname@example.com
I would absolutely love those black wedge booties! I'm also really needing a new pair of black skinny jeans.
Katy, firstname.lastname@example.com
I need some new jeans--badly!!
Those Belle booties are amazing... I'd go for those!ReplyDelete
I would love to put it towards a leather jacket. Shopbop has so many great ones!ReplyDelete
I would definitely put it towards a great new Vince sweater for fall!ReplyDelete
you read my mind! i would love a new pair of booties for the fall!ReplyDelete
Love those booties at the top. I'd probably get jeans though. I think I'm done with the low rise.ReplyDelete
redchampagne @ gmail.com
hi, what a great giveaway, i love shopbop!ReplyDelete
If i won, i would go for a rebecca minkoff mini mac bag.
Thanks a lot, Radmila, email@example.com
thank you!ReplyDelete
I need a new wallet, so I really wish for Marc Jacobs one! :)
Great giveaway! I'd probably use it towards a Tory Burch handbag. firstname.lastname@example.com
Love the booties you found! and Shopbop is my favorite site!! I would love to win that giftcard and buy some booties or this DVF dress I have my eye on!ReplyDelete
A Tory burch cross body bag I want so bad!ReplyDelete
Yup, I'd have to use that $100 on a bootie call myself. Sign me up, mamacita!! Thanks for the opportunity, Mel!!ReplyDelete
merciblahblah at gmail dot com
Love shopbop! I would put the gift card towards a new RM bag or T-Bags dress! firstname.lastname@example.com
Love! I want some gray skinny jeans. email@example.com
Oh love the booties! If I won, I would get Club Monaco Shoshana Shirt!ReplyDelete
fashionkitten03 (at) hotmail (dot) com
I love your blog! I'd spend it on a new pair of skinny jeans :)ReplyDelete
Love your blog!ReplyDelete
The Frye Vera Strappy booties are top on my fall wish list!
I think I would use it to get some new long fall tops to go with my new skinny jeans. email@example.com
I also have my eye on the Isabel Marant booties (in black.) They are amazing! If not that, I would go for a new coat. KatReplyDelete
What a awesome giveaway! I would have to pick new fall boots, since my wardrobe is in dire need of them! email@example.com
OMG! WANT them all!ReplyDelete
swankychicfete (at) gmail (dot) com
Yay!!! I'd buy a pair of your raved about 1961 skinny jeans!!! Amazing blog! firstname.lastname@example.com
Yay yay giveaway :) Love the Belle booties! I'm a sucker for blouses so I'd have to go with the Joie Marlo blouse in navy. A beautiful basic to pair with some fun accessories, skinnies, and boots! LizReplyDelete
Hi Mel! It's been so long! Hope you are doing well!ReplyDelete
What an awesome giveaway! I can think of a bazillion ways I'd like to spend $$ at Shopbop. =) Booties, jeans, fall sweaters...the list goes on!
Those booties are adorable and so versatile, but I could also invest in a pair of flattering skinnies. Maybe a nice new pair would act as motivation after having this baby (in December)! email@example.com
Amazing giveaway, Melanie!ReplyDelete
Kate, firstname.lastname@example.com
I think I'd be boring and go for a new pair of jeans. Or maybe some fun booties or flats...
What dreamy giveaway! I would definitely spend it on booties as well! I need a pair of black ones and would love to splurge on some! Thanks!ReplyDelete
- Stacey CSeptember 28, 2012 at 10:40 AM
I love Shopbop! I think I would use it toward new flats or a dress. Thanks for hosting this awesome giveaway!ReplyDelete
I would LOVE to win this!! Thank you for hosting & sharing with all of us! I have a whole list of items I need/want:) If I have to narrow down, probably on a blouse or cardigan! email@example.com
- AnonymousSeptember 28, 2012 at 11:32 AM
What an awesome giveaway!! I would have to spend it on shoes, shoes, shoes!!!ReplyDelete
- AnonymousSeptember 28, 2012 at 11:45 AM
This is a great contest! Love Shopbop! I would definitely spend the card on some non-maternity clothes, maybe a new blouse or some black skinnies. Less than a month to my due date and I can't wait to get into fall fashions! (Either adorable clothes, or a pair of booties to pamper my feet after three months of swelling and being relegated to flats.)ReplyDelete
carolineslawson @ gmail . com
I would get a new pair of jeans for fall.ReplyDelete
I would love some new black skinnies!ReplyDelete
Simple and classic ballet flats for me! Perfect for chasing after my two little boys.ReplyDelete
I'd love to get a new pair of 7 for all mankind jeans. Thank you for the giveaway and the chance!ReplyDelete
mom22girlz at ymail dot com
There are a million things I want for my fall/winter wardrobe! I hope I win :)ReplyDelete
Definitely the Jean-Michel Cazabat Rica Wedge Booties. Black, wedge, point-toe booties. That covers all the trends right? lol!ReplyDelete
LOVE Shopbop! I just pinned that picture of Gwyneth yesterday, so I'm dying over those gorgeous red booties that you found, and I would totally buy them if I won the giftcard! Fingers crossed! Thanks for hosting this giveaway! kerry.stiles{at}gmail{dot}comReplyDelete
I would love a new pair of boots! Thanks for a fantastic giveaway!ReplyDelete
- AnonymousSeptember 28, 2012 at 3:14 PM
Love your pics, Booties for me too! Or a new pair of black riding boots, I've had mine re-soled 3 times now. Probably time for a new pair! :)ReplyDelete
I would either get a new pair of black boots or a cozy wrap sweater, or maybe both!ReplyDelete
I wanna shake my booty in some red hot booties!ReplyDelete
- AnonymousSeptember 28, 2012 at 8:21 PM
Love the shopbop giveaway! I would love a pair of DL 1961 jeans. firstname.lastname@example.com
it's a huge giveaway.ReplyDelete
i can't barely wait to see who the winner is
i would like to have Alexander Wang printed lizard marion bag.
So fun!! I love Shopbop!! I would love to put the gift card toward a new pair of cute skinnies! Thank you!!ReplyDelete
Great giveaway, I would either get a pair of boots or a new handbag.ReplyDelete
Boot wedges would be perfect, just dress enough for work I think.ReplyDelete
geminicalli at yahoo dot com
thanks for the giveaway. I would buy hunter rainboots.ReplyDelete
michelle wong
mwong241 (at) gmail (dot) com
This comment has been removed by the author.ReplyDelete
I'd probably spend the oney on boots or jeansReplyDelete
Nataly carbonell
taty_bonell [AT] hotmail [DOT] com
- AnonymousSeptember 30, 2012 at 12:18 AM
The BB Dakota Zane Leather Lapel Blazer is so mine if I win this!!ReplyDelete
Thanks for this amazing giveaway :3
Monica Meza
- AnonymousSeptember 30, 2012 at 8:45 AM
I'd love a new pair of jeans!!ReplyDelete
I need a new pair of Joe's Jeans!ReplyDelete
shannoncarman at yahoo dot com
Thanks for this fabulous giveaway!!I know exactly what I would use the gift card on... a pair of good, stylish, skinny maternity jeans!! The rubber band trick just isn't cutting it anymore. :) email@example.com
- CristinaSeptember 30, 2012 at 9:17 PM
This is such a fantastic giveaway! I think I would buy a pair of Hunter boots for the rainy fall!ReplyDelete
Thanks so much!
- AnonymousSeptember 30, 2012 at 10:11 PM
I'd love to buy a new purse for the fall. Maybe one of the new wine/oxblood colored handbags. My current purse is not big enough for all of my mommy items! Such great stuff on this site!ReplyDelete
I am with you on the booties trend! Personally, I am in LOVE with the Michael Kors Wayland Suede Booties!ReplyDelete
Amy M.
Totally needing new jeans for fall! Shopbop certainly has great ones!ReplyDelete
tiffrarellano at hotmail dot com
great giveaway, I don't even know what I would get!! Fingers crossedReplyDelete
My friend and I are starting the bar method soon, I have been doing crossfit for about a year but I am ready for a change, plus you look amazing and you just had a baby!!
- Julia A.October 1, 2012 at 7:27 AM
I new pair of booties for the fall would be great!ReplyDelete
Julia A.
Oooh, yay! The red booties are TDF--love them!!ReplyDelete
I'd buy a Marc Jacobs coin purse.ReplyDelete
family74014 at gmail dot com
What a great giveaway! I could definitely use some new casual fall boots with a low heel. All of my boots are either flat or sky high. I need something in between! *Crossing fingers*! girlandtown at gmail dot comReplyDelete
Fun!! I would love some light brown booties! It would be a treat to win!!ReplyDelete
Amazing giveaway! There are so many treasures on Shopbop right now. I have my eye on the Damien top by Elizabeth and James. I also love the Joclyn heels by Nicholas. : ) Thanks for the opportunity!ReplyDelete
- AnonymousOctober 1, 2012 at 2:21 PM
Oooh I would love a new pair of shoes! The Jeffrey Campbell Celine pumps are super pretty. Thanks! :)ReplyDelete
I need new jeans! DL1961s would be the number 1 choice.ReplyDelete
Heather Pribyl
i'd get a new pair of hunter boots!ReplyDelete
I would love to get the SplendidReplyDelete
Maritime Stripe Maxi Dress: http://www.shopbop.com/maritime-stripe-maxi-dress-splendid/vp/v=1/845524441928891.htm?folderID=2534374302063654&fm=other-shopbysize&colorId=38969
Thanks for the chance to win!
wildorchid985 at gmail dot com
- CarolineOctober 3, 2012 at 1:43 PM
I'd get a knit poncho that would double as a cover up for the extra baby weight/ easy nursing cover after our first baby makes his or her debut next week! Can't wait for "normal" clothes again!ReplyDelete
I need new dress and jeansReplyDelete
I need a new cocktail dress!ReplyDelete
Hooray for a great giveaway! I need a new pair of killer denim that can be dressed up or dressed down. I hope I win :) xoReplyDelete
kim at kimberlyloc dot com
I'd love to get a pair of Minnetonka Moccasins!ReplyDelete
I'd get the Blank Denim Print Skinny Jeans:ReplyDelete
calidreamin87 (at) gmail (dot) com
- jessicaOctober 6, 2012 at 12:07 PM
I would definitely put it toward a leather jacket!ReplyDelete
This is such a great giveaway Mel! I am on the hunt for either a new fall/winter bag or those red booties Gwyneth was sporting! -- BethReplyDelete
In definite need of some new fall boots for sure! Dying over the black wedge pair!!ReplyDelete
Email for above post: email@example.com
So many things, winter basics are on my must get list. Easy separates for an on the go mom who still loves style.ReplyDelete
Thanks for the giveaway :)
J a l _ g a l @ h o t m a i l . C o m
I can definitely use some new jeans, Frye Boots or new booties this Fall!ReplyDelete
I could use some new jeans and shoes oh and a purse! I need a lot of new things!ReplyDelete
Heidi- firstname.lastname@example.com
I need a new pair of Uggs for the winter!ReplyDelete
Katey- email@example.com
I love the Pendleton, The Portland Collection Chief Joseph Towel. Thanks for the awesome giveaway :)ReplyDelete
Renee Richardson
fattybumpkins at yahoo dot com
I am glad I didn't miss this! Also glad you got to spend time your family! I like to say booties but knowing me I would probably end up buying jewelry. I have a problem. : ) stemparties@ gmail.comReplyDelete
I would love a pair of new booties as well! Great for a California fall/winter! Hope you are having a great weekend!ReplyDelete
Oh I really could use a new pair of jeans! Great giveaway. I am new to your blog and am so glad to have found it!!ReplyDelete
Enter me, please!!ReplyDelete
I'd pick these:
- courtney dOctober 7, 2012 at 11:36 AM
i would get a new pair of sunglasses!ReplyDelete
cgalloway2 (at) yahoo (dot) com
Thanks for the contest. I'd either get a new pair of jeans (really want/need...just had a baby) or a pair of Matt Bernson shoes! Thank you again!ReplyDelete


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Connecticut surpasses goal for insurance sign-ups under Obamacare
Talks ongoing to add more carriers
new haven >> Access Health CT continues to exceed internal benchmarks, with more than 100,000 residents enrolled in insurance since it started taking applications for what commonly is referred to as Obamacare.
State and federal officials Monday said 121,983 people had signed up for health insurance as of Sunday, with 50,665, or some 41.5 percent, enrolled in private insurance plans and the rest in Medicaid.
Access Health CT CEO Kevin Counihan said the organization reached its goal of 100,000 seven weeks earlier than anticipated and is the first in the country to exceed its internal goal. He said 22 percent more were enrolled by early February than were projected for the end of March.
Counihan said compared nationally, Connecticut ranks third in enrollment of those who are eligible.
"Consumers have consistently reported a positive experience with Access Health CT," said Gov. Dannel P. Malloy, who attended the announcement at the enrollment center on Church Street, with Lt. Gov. Nancy Wyman, Counihan and U.S. Rep. Rosa L. DeLauro, D-3.
Counihan said he is in talks with two to three new carriers exploring the possibility of offering plans as part of Access in November. "We want to offer more choice, more competition," he said.
The CEO said applications are being analyzed, but indications are that for the first time the state will be below 8 percent uninsured by March 31.
The projected number of uninsured is now an estimated 286,000, down from 344,000, or 9.4 percent, in 2010. he said.
"Is that the end all and be all? Absolutely not, but for the first year, it is good progress," Counihan said.
Counihan said 34 percent of applications were for people younger than 44. More details will be released next week.
As for the choice of the three available carriers, 60 percent picked Anthem Blue Cross and Blue Shield; 37 percent took ConnectiCare; and 3 percent Healthy CT.
Counihan said they expect there will be a higher percentage of residents, who signed up for Medicaid under Access Health CT, to have been previously uninsured, than those who took private insurance.
Counihan said a McKinsey analysis of the private insurance market nationally found that 11 percent of Obamacare enrollees previously were uninsured.
Pre-analysis, he estimated that somewhere between 25 percent and 50 percent of the 71,318 newly enrolled in Medicaid in Connecticut through Access were uninsured and another 5,000 to 8,000 in the private market did not have insurance.
Access Health CT said the split on enrollment is 50,665 in private insurance; 48,983 in Medicaid because of the expansion under the Affordable Care Act and an estimated 22,335 in Medicaid who would have qualified before Obamacare.
Asked how long residents have to wait for their insurance cards once they have signed up, Counihan said some are faster than others. He said "in general" it is two to three weeks. "They are getting better," he said. "They are making good, steady progress."
Counihan said Access is looking at the potential of opening a third enrollment center beyond those in New Haven and New Britain.
As for enrolling members of the Hispanic community, Counihan said a Spanish website should be up in two weeks.
At the end of December, Counihan said growing pains included three times as many inquiries to its call centers; carriers getting eligibility files for the first time ever and Anthem introducing a new billing system nationwide. He expects all those factors will have settled down by the next surge, which will run from the middle to the end of March.
Since the legislative session is under way, Counihan was asked his opinion on negotiating with carriers for better rates.
Malloy said Connecticut has the fourth-highest costs in the nation. "So we should expect to have relatively high pricing," the governor said, although he said there were some good signs for that pricing to come down.
He said some carriers dropped out when the state insurance department said it would review rates. "We are trying to strike the right balance," Malloy said. "We will have that discussion with the legislature as time goes on."
He said those higher costs are related to demographics as we are an aging state, among other factors.
Malloy pointed out that Connecticut is going to stand behind the higher Medicaid reimbursement rates for practicioners. He said 3,600 of them now take Medicaid patients, as opposed to only 1,200 two years ago.
The governor was asked about obstacles to people coming in and signing up for insurance. He said for one thing the undocumented are not eligible, although they can get care at community health centers.
Counihan said across the country about 3 million people have enrolled in private insurance through the federal and state exchanges and 6 million to 7 million in Medicaid. "This law is not going to be defunded. It is not going to be repealed," he said.
Counihan said service through Access Health CT is getting better, as call center staff has doubled since December. He said in the beginning the wait times were 45 minutes and the abandonment rate was 37 percent. Now, the abandonment rate is less than one percent and the average wait is down to 16 seconds. "We are making progress," Counihan said.
Malloy said there are 465 certified navigator assistants and certified application counselors providing in-person assistance to more than 160 faith-based and health community-based organizations.
Have questions, feedback or ideas about our news coverage? Connect directly with New Haven Register editors at AskTheRegister.com.


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Established 1976 in Denver, Colorado. Circa 40,000 volumes in stock. By Appointment only, however your are most welcome to come by, so please email [email protected] for an appointment.You can call us at 303 733-5700 but we are usually away from the phone. Leave a message telling us what the call is about, and will call you back. We have 75,000 Rare, Scarce, and Out-of-print Books, Ephemera,and most areas are covered including Americana, Western Americana, Civil War, Military, World War 1, World War II, Children, Illustrated, Science, Technology, Math, Physics, Engineering, Geology, First Editions Fiction, Books on Books, Bibliographies, Art, Theater, Natural History, Travel, Britain, Cookbooks and more.
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A week after hackers revealed the potential identities of millions of people with accounts on the Ashley Madison adultery site, the NBC Connecticut Troubleshooters have learned five of those leaked accounts appear to be linked to the email addresses of Hartford city and public school employees.
The revelation may prompt some city policy changes. Hartford auditors told the Troubleshooters that next month, they will recommend that the city government and school system block city server access to Ashley Madison, along with dating sites such as Match.com and eHarmony.
Hartford's internal audit department has identified five email addresses from the city and public school system used to sign up for Ashley Madison, mostly during 2011 and 2012.
The five include two email addresses from the Hartford Police Department, two from Hartford Public Schools and one linked to the Hartford Public Works Department.
Auditors said about 600 emails from Ashley Madison were sent to those email addresses.
It's not clear if anyone read the Ashley Madison messages or for how long. Auditors also have not asked the employees if they personally created the Ashley Madison accounts or if someone else may have used their email addresses.
Auditors also pointed out that city policies permit employees to use their government email accounts for "brief and infrequent" personal matters, so the people identified who still work for the city likely will not be disciplined.


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Are you a current Berkeley student looking for ways to get more involved with the Jacobs student community? This page describes some avenues for you to help build the community at Jacobs Hall.
The Jacobs Institute Student Advisory Board (SAB) helps to ensure that the opinions, needs, and interests of the student community are taken into account during strategic planning and program development. Though the Jacobs Institute may not be able to act upon all of the suggestions offered by the SAB, its input will be taken seriously. SAB members should be invested in the design community at Berkeley and represent diverse perspectives from the student community. Members commit to:
SAB members receive a complimentary Maker Pass during their tenure and are listed on the Jacobs Institute website. Students can stay on the SAB for multiple years, provided that they can fulfill board commitments. We recruit new board members at the beginning of each academic year.
The Advanced Prototyper program is a volunteer program for the experienced Maker Pass holder who is ready for the next challenge. Are you ready to take the skills you have learned in the makerspace and give something back to your peers? Do perks like a free Maker Pass, lockable storage, and increased access sound exciting to you? Then apply for the program and we will be in contact with you about next steps! To be eligible for the Advanced Prototyper Program, you should:
The Jacobs Student Supervisors are undergraduate student staff who are responsible for helping Jacobs Hall run smoothly and safely. Primary responsibilities to achieve this include monitoring makerspace access, enforcing safety and usage policies, regularly maintaining resources, and providing assistance to Maker Pass holders. Experienced Student Supervisors have the opportunity to advance to the Student Supervisor II role, which entails additional equipment maintenance and training responsibilities; each Student Supervisor II apprentices with a Jacobs Design Specialist and gains specialized knowledge of a particular area of the makerspace. New Student Supervisors are hired in August, and occasionally in January, before the start of the semester. If you are interested in joining the Student Supervisor team in the future, contact Mary Catherine Richardson, Design Specialist and Manager, Student Supervisor Program.
A small team of students supports the Jacobs Institute's work in graphic design, content production for web and print, and creative storytelling. Graphic Design Assistants primarily focus on design outputs like posters and print collateral, illustrations, infographics, and brand applications, while Student Reporters primarily focus on outputs like written stories, video and photography, and web and social media content. Both roles involve the opportunity to work on long-term special projects, propose new ideas, and work as part of a collaborative team. Positions on the Jacobs creative team are currently filled.
The Program Assistant works with the Jacobs program staff to help courses and programs at Jacobs Hall run smoothly. The primary responsibilities to achieve this include maintaining class, event, and office supplies, formatting announcements for the student community mailing list, supporting event marketing and operations, and maintaining our collection of collateral and student projects. This position is currently filled.
Each semester, the Jacobs Institute hires dedicated, knowledgeable graduate students to serve as academic student employees (ASEs) for the Design Innovation (DES INV) courses. Available roles range from graduate student instructors (GSIs), readers, and tutors, depending on the course.
Interested students should apply through the ASE application portal. Please review information about the course(s), including meeting time and content, before you apply. Course instructors manage the search process for their ASEs and will contact applicants whom they would like to interview. We aim to fill spring 2020 roles by October 31st.
Spring 2020 ASE positions
To join the general candidate pool and receive an email when the ASE application opens for the next semester, fill out this interest form. To learn more about graduate student academic appointments, including fee remission policies, visit the Graduate Division website.


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A 5.9-magnitude earthquake has rattled New Zealand, panicking shoppers and residents but causing no major damage.
The quake hit at 3:36 pm local time with its epicentre about 66 kilometres from the South Island town of Kaikoura at a depth of 55 kilometres, the US Geological Survey said.
The local GeoNet monitoring service measured the quake at 6.2 and said it was felt across the entire country, but was unlikely to cause damage because it was so deep.
"I am advised that MCDEM (emergency management) hasn't received any reports of major injuries or damage," Civil Defence Minister Nikki Kaye said.
However, there were widespread outages on landline and mobile networks, while some rail services were briefly suspended as tracks were inspected for damage.
Kaikoura District Council chief executive Stuart Grant told TVNZ the quake felt like two tremors in quick succession, describing the second as a "big jolt".
He said the council building was evacuated as a precaution but appeared undamaged.
The manager of Kaikoura's Mitre 10 hardware store James Hills said the quake dislodged items from shelves and sent panicked customers fleeing from the building.
"(There was a) fair bit of panic... everything was falling off the shelves," he said.
"There's been a little bit of damage, certainly not heaps, but yeah, there's a lot of stuff fallen over."
Local resident Caleb McNabb said on Twitter that buildings "swayed" in Kaikoura's main street, while Wellington's Mandy Simpson tweeted "that was a long, horrid shake".
The quake struck in the same region as a 4.7 tremor yesterday that jolted office buildings in Wellington.
There were also two 6.5-magnitude quakes around the same area in July and August 2013. GeoNet data manager Kevin Fenaughty said it was unclear if there was a direct relationship.
"We cannot be certain that it hasn't been triggered by stresses from all those quakes over time," he said.
A devastating tremor hit the South Island city of Christchurch in February 2011, killing 185 people.
New Zealand is on the boundary of the Australian and Pacific tectonic plates, which form part of the "Ring of Fire", and experiences up to 15,000 tremors a year.


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The return of one of the Turkish Airlines EuroLeague's greatest coaches, David Blatt, to his adopted country of Israel
Apr 01, 2019·24 : 22
What is the status of gender equity at the highest level of European professional basketball?
Mar 13, 2019·26 : 45
The biggest surprise of the current Turkish Airlines EuroLeague season, playoffs hopeful FC Bayern Munich, opens the fourth episode of The Journey, a behind-the-scenes broadcast series tracking five teams in the competition from the opening tip until this season's champion is crowned.
Feb 15, 2019·24 : 30
Symphony in Yellow reunites Maccabi's back-to-back EuroLeague champions
Feb 11, 2019·41 : 21
Euroleague Basketball Originals: The Journey (Episode 3)
Jan 24, 2019·24 : 29
Inside the Greek Derby: The Journey, a behind-the-scenes broadcast series that is tracking five Turkish Airlines EuroLeague teams from the opening tip until this season's champion is crowned, opens its second episode going into the heart of one of world sport's most intense rivalries.
Dec 14, 2018·24 : 08
A Euroleague Basketball Originals production, The Journey brings you closer than ever before to the action. This newly created series will focus on five different stories that have one element in common: pursuing success.
Nov 11, 2018·24 : 40


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Is your Club already affiliated?
Find your club on our list of affiliated clubs here >
Renewal of your annual affiliation is due on April 1st each year.
Download a Club Affiliation Renewal Form 2019 -20
Interested in affiliating your club?
Affiliation is a partnership – we support you and your club members and in turn we expect you to get your club engaged in our activities. The affiliation process begins with a phone call chat, then a visit. If appropriate, we may then put your club forward to our Executive Committee for their approval to affiliate.
Download a New Club Affiliation Form 2019 -20 >
Find out more about our clubs and the benefits of affiliation >


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Ben-Hur Cut Down Trailer (2016)
Alternate trailer for Ben-Hur shows additional footage not shown in the trailer.
Remake of the 1959 classic, a falsely accused nobleman survives years of slavery to take vengeance on his best friend who betrayed him.
It would seem the casting agent for Ben-Hur always knew the character Esther would be extremely attractive. While Nazanin Boniadi is definitely that, the role was also offered to Sofia Boutella, Moran Atias and Natlia Warner as well.
Remakes of classics rarely make sense, and the 1959 Ben-Hur has been ranked #2 by the American Film Institute for greatest film under the genre of "epic". Because of this, we are presented a double-edged sword. Since regarded so highly, studios see the need [and cash dollars] to re-create the story for the next generation. But, at the same time, the original is so good that it is unlikely for the remake to surpass it or even match it.
As shown even in this Ben-Hur trailer, the franchise is known for its insane chariot races. The 1959 version required an infirmary to be built near the set, to take care of injuries incurred during the race sequences. Fortunately, the infirmary spent most its time healing sunburns.
Two trailers for a highly anticipated film in a single day? Immediately after releasing the first trailer for the film, Paramount followed up with this "Cut Down" trailer. Taking advantage of a shorter running time, the condensed trailer seems to have more caffeine pumped into its veins while it focuses on visuals and less on story.
It makes sense, and we can imagine future TV spots for Ben Hur to feel a lot like this. Just because most productions featuring the always-eloquent Morgan Freeman feel the need to use his soothing voice, this trailer made sure to have his character, Ilderim, play narrator.
1 min 30 sec
Posted On
March 16, 2016
Timur Bekmambetov
Lew Wallace
Paramount Pictures
August 12, 2016
Jack Huston
Nazanin Boniadi
Haluk Bilginer
Pilou Asbæk
Marwan Kenzari
No Music Available


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Join us on our new FB Group:
Campbell River Minor Softball/Fastpitch
All players MUST have a Softball BC Number.
If your child does not have one, go online http://www.softball.bc.ca/ and sign up.
It is a one time fee of $10.00, then that is their number for life. A player must have this to complete registration.


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Drosera filiformis complex plants. From left to right: Drosera filiformis var. floridana, exceptionally red plant, Washington County, Florida. D. filiformis, Columbus County, North Carolina. D. filiformis, Ocean County, New Jersey. D. x californica 'California Sunset'. D. tracyi, Walton County, Florida. The plants were grown next to each other outside. All plants blooming size except the Drosera tracyi.
Drosera filiformis and Drosera tracyi are two closely related North American species. Drosera filiformis is found on the Atlantic coast from North Carolina to Nova Scotia along with a few populations in the Florida panhandle. The Florida plants are generally smaller and redder than the Atlantic coast plants and were given the name Drosera filiformis var. floridana. The ICPS Seed Bank has distributed seeds of Drosera filiformis var. floridana as "Florida all red", "Florida red", or simply "Florida". Drosera tracyi is found along the gulf coast from Louisiana to the Florida panhandle and southern Georgia. Both species form hibernacula in the winter although Drosera tracyi is found in an area generally considered warm temperate.
Hybrids between Drosera filiformis and Drosera tracyi are easy to make partly because Drosera tracyi flowers tend not to self pollinate very well. The origin of the hybrids Drosera 'Florida Giant', "Carolina Giant", and 'Portland Sunrise' are unknown. Drosera 'California Sunset' was produced by one of the founders of the ICPS. The hybrids tend to be much easier to maintain in cultivation than either of the parents and it is quite possible that many "Drosera tracyi" plants in cultivation have some introgression from Drosera filiformis. Hybrids between Drosera filiformis and Drosera tracyi may be referred to as Drosera x californica.
The Atlantic coast Drosera filiformis requires a definite winter season to survive long term. You should grow them outside year round if you live within their natural range of USDA zones 6 and 7. If you live in a colder region, move the plants into a protected area after they have gone dormant. It is especially important they have protection from late spring frosts. If you live in zones 8 and 9 the plants should do well. If you live in zones 9 or warmer grow the Drosera filiformis var. floridana form outside. There are selected clones of Florida Drosera filiformis that will survive outside in zone 7.
Drosera tracyi is found in USDA zone 9. It requires winter protection if grown in a colder climate and is especially sensitive to early and late freezes. We had trouble growing it outside in zone 9 in northern California which may have been a result of no real winter and long wet springs. Consider growing Drosera x californica 'California Sunset' or other hybrids between Drosera filiformis and Drosera tracyi instead. The hybrids can handle winter better than Drosera tracyi. Or grow Drosera tracyi in a cold greenhouse.
If you want to grow Drosera filiformis in a terrarium, it is best to grow the Drosera filiformis var. floridana form. The seeds germinate without stratification and some of the plants do not go dormant. Most will form loose hibernacula that last a month or so before the plant starts growing again. The more you feed them the more likely they will not go dormant. This plant may be grown outdoors in summer but needs to be in a warm greenhouse or other location that does not freeze during the winter. In a terrarium the Florida form may not develop the deep red color it would in full sun outdoors.
Seeds of Drosera filiformis and probably Drosera tracyi need a "winter" to germinate. The usual way to do this is to give the seeds a few weeks of damp cold stratification. You can put pots planted with the seeds in the refrigerator for 4 weeks or just put them outside if you live where it gets cold during the winter. An alternative is to put the seeds in a small plastic bag with damp peat or finely chopped sphagnum moss in the refrigerator. The seeds and moss can be carefully spread over the surface of the soil in a pot after stratification. Probably the best way to start these species from seed is to put pots planted with seeds outside in the winter and allow the seeds to sprout naturally. That way the new plants are immediately in phase with the seasons and will not go dormant prematurely.
No matter how you handle stratification, the seeds should not be buried when you want them to germinate. A pot with a 50:50 mix of peat and sand usually works well. Drosera filiformis and Drosera tracyi like large tall pots and can rot if the surface of the soil is too wet. You can mix extra sand into the top 1 cm of soil or even use pure sand the thickness of the hibernacula. The pots should always be sitting in water. Please see Sowing Seeds Step-by-Step for more details on starting seeds and Growing cold temperate Drosera for more information on growing the plants keeping in mind that Drosera tracyi is rather cold sensitive.
Make sure you feed your plants well if they are not catching many insects on their own. You can use rehydrated dried blood worms or foliar feed the plants with a dilute liquid fertilizer.
-- John Brittnacher
For more information please see:
Robinson, James T. (1981) New CP Cultivar Received in 1980. Carniv. Pl. Newslett. 10(4):95 ( PDF )
Sheridan, Philip (1978) New Jersey Pine Barrens. Carniv. Pl. Newslett. 7(4):107-109 ( PDF )
Schnell, Don (1995) Drosera filiformis Raf.: One species or two?. Carniv. Pl. Newslett. 24(1):11-15 ( PDF )
Hummer, John H. (1998) A noteworthy Florida find: Drosera filiformis var. tracyi (Droseraceae). Carniv. Pl. Newslett. 27(3):89-90 ( PDF )
Rice, Barry A. (2011) The thread-leaf sundews Drosera filiformis and Drosera tracyi. Carniv. Pl. Newslett. 40(1):4-16 ( PDF )
Drosera 'California Sunset' flower. This plant is a hybrid between Drosera filiformis and Drosera tracyi.
Drosera tracyi in a 8 cm pot.
Drosera filiformis var. filiformis flower.
Drosera filiformis var. floridana is smaller and more delicate than Drosera filiformis var. filiformis.
Drosera filiformis var. filiformis is smaller and more delicate than Drosera tracyi.
Drosera tracyi up close. Be prepared to be covered in slime when working with this species.
False vivipary on a Drosera filiformis scape. Note the scape is branched as is one of the leaves.


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Friday, May 13, 2016
Relaxation Time
Use Shabbat as a time to relax and separate yourself from the age of constant information.
Posted by Jewish Treats at 1:00 AM
Labels: Action
1 comment:
- Leslie Lim said...
I read your blog.I thought it was great.. Hope you have a great day. God bless.
- June 23, 2016 at 1:12 AM
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The anatomy of a Disney movie used to be simple: princess meets prince, evil witch wreaks havoc, prince is hero, prince gets the girl. Things were black-and-white. A fairy-tale was nothing but a story.
Increasingly, we're seeing more complex stories emerge that more accurately reflect real life. Sometimes the ugly one isn't the villain, sometimes the handsome man is not the hero, sometimes romance is not the answer. Maleficent is the latest of these stories. It humanises the "badie" to the point where we're forced to reconsider the very nature of evil.
I thought I was tired of re-tellings of the same story – does there seems to be a dearth of creativity in Hollywood at the moment, or is it just me? After hearing the reviews, I was braced for another Oz: The Great and Powerful (powerful disappointment, maybe), but Maleficent surprised me. Seeing it for the first time on Blu-ray, I loved it. However, I'm not sure that it hits the nail on the head for its target audience: children.
The story opens by establishing a complex political landscape. The first act is about forbidden love (easy enough to grasp), followed by intense betrayal (the kind that I'm not sure I'd want my kids to witness). Then the familiar story of sleeping beauty starts, featuring annoying fairies (now ten times more annoying) and a surprising lack of animal companions (this is still a Disney movie, isn't it?).
Sleeping Beauty's villain has always been among the most graceful and enthralling Disney characters and no one can carry off that level of poise and malice like Angelina Jolie. The other star of the show is the CGI which creates rich, layered environments. There's something about the world where this film plays out that is both realistic and fantastical. Rich colours and high saturation combined with striking attention to detail creates a visual feast that's difficult to look away from.
It's a good analogy for the story too. It's more nuanced than your average Disney with some attempt at creating real, three-dimensional, characters and with real driving motivations. On the other hand many of the characters are still the pure fantastical archetypes with completely unrealistic reasoning and motivation. Basically you're more likely to enjoy it if you focus on the titular character and enjoy the prettiness.
Praise must also be given for the regular homages to the 1959 animated film, Sleeping Beauty. Disney fans will pick up familiar snatches of dialogue and some scenes – in particular the famous curse scene – are filmed to be almost identical. A new, haunting, version of Tchaikovsky's Sleeping Beauty Waltz – the main song from the 1959 flick – plays over the credits.
If you are looking at purchasing the Blu-Ray, you can be assured that the stunning visuals and dramatic score do the format justice. In addition, the release has a number of special featurettes on the making of the film that are short but interesting.
It's the usual fare – interviews with the cast where they speak about how wonderful the other members of the cast are, a few deleted scenes and some information on how the story came to be. The most interesting of these features to me was a look at how the CGI was done. What was disappointing is that they didn't really go into the costumes or character design. There's a clip on Maleficent's head dress but nothing more.
This is by far the darkest Disney story I've ever seen, and I guess that's part of its appeal. The age rating is 7 – 9 PGV, but I'd caution parents of sensitive little ones against buying this one. The monsters are very scary and there are sad bits that had me – as an adult – in tears (don't judge). As the age rating suggests, there's also a lot of general violence. That said, it's good to see a strong and powerful female lead character, and a message that doesn't imply the ultimate aspiration for any little girl is to be rescued by a muscly man.


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On Fri, 12 Aug 2005 14:18:45 +0200, John Foliot - WATS.ca <email@example.com> wrote: > In a 1997 CHI (Computer Human Interface) paper [2], it was noted, "The > basic insight is that, in order to navigate through a world with minimal > prior knowledge of its layout, .... that they [developers - JF] shall > not overwhelm the user with information. In particular for view > navigation, Furnas showed that it is ideal to show only small views (a > relatively small number of choices) that the number of navigation steps > is not too large and that the route to any target must be discoverable." > A Microsoft study [1.2] demonstrated that accuracy diminished as more > "sub-levels" (hierarchy) were added (in other words one nested list > inside the master list is preferred over a list inside of a list inside > of a list). > However, George A. Miller (the original author of "The Magical Number > Seven") noted "The point seems to be that, as we add more variables..., > we increase the total capacity [of differentiation - JF], but we > decrease the accuracy for any particular variable.."[1.1] There's some interesting work done by (among others) Inmaculada Fajardo Bravo at the Univesity of the Basque country, which suggests that there are different cognitive paths taken by "most people" and "most pre-lingually deaf signers". I don't have a reference handy, but it should be findable (I think I have pointed to it on this list in the past). The general point of cognitively overloading a page is pretty sound though, IMHO. There is a trade-off between being able to navigate to anywhere, and being able to work out how to get to where you want to go... and too much choice isn't often helpful. Good site design instead employs multiple paths to the same information, as well as information about "_the_" path (as understood by the site designer). That's why the Web is designed as a web, not a directory tree, although all too often it is built to look like a directory tree. cheers Chaals -- Charles McCathieNevile firstname.lastname@example.com hablo español - je parle français - jeg lærer norsk Here's one we prepared earlier: http://www.opera.com/downloadReceived on Friday, 12 August 2005 13:29:37 UTC
This archive was generated by hypermail 2.3.1 : Tuesday, 13 October 2015 16:21:32 UTC


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P0626 OBD-II: Generator Field/F Terminal Circuit High
Get diagnosis and repair information for trouble code P0626
OBD II Fault Code
OBD-II Code Engine may not start is defined as a Generator Field/F Terminal Circuit High
The Alternator recharges the Battery when the engine is running. The Alternator is controlled by the engine control module (PCM). If the PCM detects an incorrect signal from the Alternator, the PCM will set code P0626.
Engine may not start Symptoms
- Engine may not start
- Engine may stall
Common Problems That Trigger the P0626 Code
- Alternator failure
- Battery failure
- Powertrain Control Module (PCM) failure
- Wiring issue
Common Repairs Needed for the P0626 Code
Not the OBD-II Code You're Looking For?
More OBD-II Codes


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It is engaged in providing state of the art learning technologies to its clients. We make use of Neuro-Linguistic Programming (NLP),defined as science of achievement to enhance effectiveness of all the programs offered by us. We also offer core NLP programs conducted by some of the best trainer in the world.
Wisdom Tree takes pride in having its client base in different segments, and has successfully delivered the products for their requirements.
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Achieve your goals and success in life...discover a better you!
In case you have any queries, write to us at firstname.lastname@example.com
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|Rediff India Abroad Home | All the sections
Discuss | Email | Print | Get latest news on your desktop
Ahead of final phase, ultras kill 2 CRPF men
December 22, 2008 18:43 IST
Two days ahead of the seventh and final phase of the Jammu and Kashmir [Images] assembly poll, militants on Monday shot dead two Central Reserve Police Force soldiers and fled with their service rifles in Baramulla district.
Militants sprayed bullets on constables Inderjeet Singh and S S Budhria from a point blank range in Iqbal Market near the main bus stand in Sopore town, official sources said.
Both were killed instantly, they said adding the ultras took away their rifles before fleeing.
The shooting sparked panic in the North Kashmir town, 55 km from Srinagar [Images], which was immediately cordoned off by security forces to apprehend the attackers.
No militant outfit has claimed responsibility for the attack.
The incident, the first major strike by ultras in recent months, took place at a time when security had been beefed up across the state to ensure peaceful polling in the last phase.
As many as 21 constituencies are going to polls in the last lap on December 24.
Email | Print | Get latest news on your desktop


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Part 2 of this article describes the regulatory framework for prosecuting and defending election contests (including relevant U.S. Securities and Exchange Commission staff interpretations and filing requirements) and the tools used by contestants to wage their fight campaigns. Part 2 also offers certain strategic and tactical recommendations to would-be contestants and describes various factors to be considered for the settlement of election contests.
June 05, 2015 Published Article Law 360
Shareholder Activism And Defense Strategies: Part 2
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I know, I haven't posted in awhile and here I am (eek) reblogging. But doesn't this look delightful dear readers?
New things in the works for us over here at ZH. Stay tuned (and make this seriously AH-MAH-ZING dish! – with your kids of course!!)
Wagamama serve a dish called Chicken Katsu Curry. I have never actually eaten it, but in theory it sounds wonderful. Crispy, breaded chicken with a flavourful curry sauce and fluffy rice.
As we are trying to save money, rather than head out to try this dish, I thought I'd make my own version at home. It was all guess work really, but it turned out well – hubby declared it 'restaurant quality', but he is the biggest fan of chicken in any sort of crispy coating so he is biased.
The chicken and curry sauce worked really well together. It's not the sort of meal you could have every night, but for a hump day treat, why not give it a go?
Try this recipe, and if you've had the Wagamama version you'll have to let me know if it's at all similar 🙂
This will serve 2, and takes…
View original post 257 more words


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Review Details
AlcoSense Elite 2 Breathalyzer (Multi Alert Level)
- Product Review (submitted on 20 August 2013):
- Drink driving is never a good idea, but sometimes, we don't even know ourselves if we're over the limit.This Alcosense Elite is probably the most important item I carry in my car. I've used it after a heavy a night many a time. After testing it on myself, friends, and family, it does sometimes give surprising readings, but it also acts as a real eye opener. I've allowed myself to wait a little longer before driving after testing myself a few times.To me, this doesn't just save your license, it could even save your life.I usually post reviews on books, games and films, but drink driving is something I am strongly against, and this Alcosense unit is nothing but a good idea, especially at its the reasonable price!


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Saigon is a city torn: capitalism pulling it towards the developed world while the communist regime keeps it trapped in the past. So we left Ho Chi Minh City in yesteryear and moved north along the South China Sea.
First stop, the massive dunes of Mui Ne. Having not set an alarm, we awoke with the sun having turned our room into a sauna. A short while later, I negotiated a rental of a sick Honda Airblade moped for our exploration of the coast line. Thirty minutes along a deserted road and a fishing village's bay jammed with junk boats revealed itself. The sight caught us off guard, what with all the uniform blue masts and yellow trimmed hulls. Further along, the unmistakable vision of giant sand dunes came into focus. The pavement gave way to a shifting granular surface that required Ash to walk while I carefully navigated the machinery across. We threw some Dong (Vietnamese currency) to three kids hanging around to safeguard our "hog†while we hiked the white dunes.
Approaching an oasis pond full of blooming lilypads, we rented a rolled up piece of teal plastic that would serve as a sand sled. Twenty minutes uphill under the blazing sunshine and we reached our destination. We peered over the edge only to have the brilliant sand sculpture dissolve beneath our toes. I scoped out the sledding hill void of any snow and gave it the 'ole college try only to humiliate myself by sliding all of five feet. Having learned from my initial failure, the second attempt can only be classified as a mild success. We opted out of additional winter sports atop the neighboring red sand dunes. It broke our hearts, but we reluctantly handed over the keys to the Honda Airblade and climbed to the top row (the bottom was crawling with roaches) of a sleeper bus bound for Nha Trang.
Our hopes were high after reading that Nha Trang was Vietnam's beach "paradise.†Our expectations were soaring. No beach could have lived up to the image conjured up in our minds. Yet we made the most of it by drinking "3 3 3†brand beers (pronounced 'Ba Ba Ba'), consuming freshly grilled squid kebabs, and relaxing at the Sailing Club. We booked a two tank scuba dive at Hon Mun ($40!), located a short distance from Nha Trang. Being the only two certified divers, Ash and I were treated to a private guide of Vietnam's intriguing underwater world. Armed with a digital underwater camera, the ocean became our playground. For a reason unbeknownst to me, Ash began an underwater flying exhibition, her arms out as if she was performing the angel balance. Chuckling with a respirator in your mouth and mask suctioned to your face causes some serious problems when forty-five feet beneath the surface. But I had no choice but to laugh.
We came across a zebra striped sea snake slithering across the ocean floor, examined straw coral, swam alongside a turtle, and even had starfish in our hair. Just when we thought we had enough fun, Huy (our diving guide) instructed me how to fill my BCD vest with enough air to stand upside down underneath the dive boat. It was an odd feeling being underwater, feet firmly against the hull, and breathing normally. Ash and I agreed that it wasn't the best diving, but certainly the most fun. It was supposed to be a quick jaunt back to Nha Trang. That was until the engine refused to start and the dive boat had to be towed back to shore. So Nha Trang wasn't all it was cracked up to be. Could have fooled us.
One long overnight bus later, we settled into the tailoring capital of SE Asia, Hoi An, Vietnam. It was Ashley's dream come true for four days of non-stop shopping. The town itself is beautiful with its French architecture painted a faded burnt yellow. Some roads are paved, others cobblestone, and most are dirt. Yet they all offer the same product: cheap, custom fit, quality clothing. The first day was spent examining twenty plus shops comparing craftsmanship, cost, and specialties. We hit the streets early the ensuing morning negotiating prices, trying on prototypes, and being fitted to ensure ample time was available to evaluate our goods the next two days. All this shopping made the man accompanying this ravenous woman thirsty. Ah… I discovered a local beer joint offering mugs of refreshing lager for just $0.18! Ash was happy, I was even happier. With cold and cheap beer at our fingertips to keep the good times going, behold our shopping spree spoils:
- Three dresses (deep blue, pink, baby blue) for Ash and her sisters (Kiley & Bailee) – $15 each
- White wool winter coat with blue silk insert and embroidered (Ash) – $55
- Long pink casual shirt (Ash) – $18
- Gray pinstripe cashmere suit with embroidery (Greg) – $90
- Navy blue pinstripe cashmere suit with embroidery (Greg) – $90
- Button down shirt (Greg) – $12
- Long light blue dress (Ash) & long yellow dress (sister-in-law Kim) – $22 each
- Deep blue silk cocktail dress (Ash) – $45
- Black travel shorts (Ash) – $22
- Brown corduroy blazer with embroidery (Greg) – $30
- Button down boy shirt (Ash) – $12
- Blue silk nightgown with our friend Amy's (recently married) new initials embroidered – $19
- Shipping clothes & assorted items to three different USA addresses – $120
We treated ourselves to all of Central Vietnam's offerings: dune sledding, underwater merriment, and a brilliant new wardrobe. A breath of fresh air… until troubling Hanoi.
- Greg and Ash
Learn From Our Footsteps:


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Released in theaters last spring, this movie makes a strong and compelling argument for a plant-based diet. The film follows three doctors who use plant-based diets on their patients as a remedy for their health problems, and the results they get can be absolutely astounding.
By now, most of us are comfortable with the concept of Dumpster diving, even if we're not willing to dive in ourselves. "DIVE!" follows the filmmaker and his friends as they salvage edible food from the Dumpsters of Los Angeles food markets and discover the enormous amount of food that's unnecessarily going to waste every day.
"Dive!" Trailer from Compeller Pictures on Vimeo.


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"Our Oshawa" is an annual publication produced by the City of Oshawa featuring the past year's major achievements and community partner accomplishments.
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Emergencies can happen anytime, anywhere. Severe weather such as floods, tornadoes, ice storms, and accidents such as chemical spills can strike any community. Learn how you can be prepared and stay informed.
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RTIwala Team answers all your question about Priya Ranjan Dasmunsi! As you may have been reading or hearing about the sudden demise of Priya Ranjan Dasmunsi on news channels and news websites. We're going to tell you about Who was Priya Ranjan Dasmunsi? Top 7 Unknown Facts about Priya Ranjan Dasmunsi which you should know!
Who was Priya Ranjan Dasmunsi?
He was a senior Congress party leader, former Union minister and MP from Raiganj constituency in West Bengal. He was survived by his wife Deepa Dasmunsi and son Priyadeep Dasmunsi. Dasmunsi was President of Indian Youth Congress in West Bengal from 1970 to 1971. The late leader entered the Indian Parliament in 1971.
Related: What is AIPC or Professionals' Congress?
He was sworn in as Union Minister of State, Commerce in 1985. Late Priya Ranjan Dasmunsi was the cabinet minister in Ministry of Parliamentary Affairs and Ministry of Information and Broadcasting during the first term of Prime Minister Manmohan Singh.
When and How Priya Ranjan Dasmunsi Died?
The Congress Statesman suffered a massive stroke on October 12, 2008. which left him partially paralyzed and unable to speak. Blood supply to a part of his brain was cut off causing irreversible damage. His body systems were functional but he breathed through a tracheostomy tube attached to his neck and was fed through a PEG tube in his stomach. All basic life functions like breathing, blood pressure, sleep-wake cycle were stable but he was not conscious of his surroundings.
Also Read: Most Expensive Mobile Phones gifted to Ministers and Govt Officials
Mr. PR Dasmunsi (72), died at 12.10 p.m. today (Monday). He had been critically ill for the past one month and unfortunately succumbed to his illness, said Apollo Hospital in a statement.
Top 7 Unknown Facts about Priya Ranjan Dasmunsi
- Late Dasmunsi was the first Indian to be a match commissioner in a World Cup match in 2006.
- Dasmunsi also served as the President of the All India Football Federation (AIFF) for almost 20 years.
- Dasmunsi was President of Indian Youth Congress in West Bengal from 1970 to 1971.
- He entered the Indian Parliament in 1971. He was sworn in as Union Minister of State, Commerce in 1985.
- The whole expenditure and all hospital bills are paid by Government of India.
- Priya Ranjan Dasmunsi's dream to set up an AIIMS-like hospital in Raiganj remained unfulfilled.
- His wife Deepa Dasmunsi contested against her brother-in-law brother Satyaranjan Dasmunsi and won the 2009 LS election from the Raiganj constituency in West Bengal by TMC.
Also Read: Rw Reveals Top 10 Unknown Facts of Mahatma Gandhi
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Please forgive the absence of an actual post today in light of the fact that I'm at the Storyline Conference, the one that I raved about a few weeks ago and am still trying to register the reality that I'm actually here. It's jam-packed with great sessions and events so instead of trying to write a deep and meaningful blog on my phone in the next 30 minutes I'm just taking a rain check on todays post. Thanks for understanding.
P.s. For those of you who have read Donald Millers books, last night Bob was here and I got to hug him. It was awesome.