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Any brothers know of a reputable source for research liquids in Canada? There are many but is hard to find quality reviews for them. I know sarms1 is the go to on this forum, but would rather not pay for the shipping if I don't have to.
Any brothers know of a reputable source for research liquids in Canada? There are many but is hard to find quality reviews for them. I know sarms1 is the go to on this forum, but would rather not pay for the shipping if I don't have to.
I live in Canada and personally use sarms1.com. I don't know of any Canadian based SARMS companies.
I have ordered from them countless times and never had a problem with receiving a package.
Well there are ones like canadianmedsupplies and researchliquids but just don't know if the product is as pure as sarms1.
I would just stick with Sarms1 to be safe. I have never had an issue with shipping. I get my pins from canadianmedsupplies but never SARMS. The problem is that there is no way for you to test them so that's where the risk is.
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It's never a sure thing guys.
I had peptides seized at the border and the stuff is legal.
It's not a huge risk, but still a risk. Since losing hundreds of $$$, I'm done ordering from the US.
And this is kinda what I was thinking too. I have no problem ordering from sarms1 apart from the fact I don't want to pay extra shipping and I dont want to run a risk of it being seized, no matter how small the risk is. If I can't find a reputable source in canada, I will use sarms1.
Don't be stupid man...
And no place is gonna offer you free shipping, where did you come up with that idea?
I've talked to a few (and that means just that, a few, 2-3 tops, not like 20 people....) big real life guys who've gotten impressive results from a few sarms. I forget which ones. I'm not saying they can't produce results but the safety claim made about them bothers me the most as it's not being truthful.
I also don't turn a blind eye to the fact I stay on about 700ish mg test year round I could be taking years off my life. I accept that, but sure as hell don't tell others to do it claiming it's safe!
Anyway, I got some injuries taking me out the next while, I may try the ones touted to help injuries. Peptides were said to do that and I'm not so sure they delivered as promised for most. That's why I have 0 expectations if I do try them, not even sure I will. At least they're not injects which makes them more user friendly.
Not saying anything about free shipping, just that shipping is cheaper in canada.
As for the safety of sarms, you make some very good points. I would most of all like to talk to an unbiased doctor regarding their use but this proves difficult. But even then, what is a doc going to be able to say about long term safety.


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Hemp is used to make rope among other things. It is also the source of what we call marijuana. I have used it off and on for many years with intervals dictated by jobs I held. I had about a 20-year lapse for a job in the oil field I held.
Now that I am retired I am fighting a battle to make cannabis legal in Texas under the auspices of NORML. I will no longer smoke cannabis until it is legal in Texas. In its place I will use a low THC, high CBD oil that I will use in my diffuser to enjoy the medical benefits of cannabidiol as an essential oil. CBD oil can be ordered on the internet.
Even very famous doctors like CNN's Sanjay Gupta are calling for marijuana's descheduling by the FDA. The evidence that marijuana is a danger to the user is simply not supported by the research. The ban on experimental studies of cannabis is not supported by any evidence. This means any kind of law against cannabis as a recreational drug is not supported by the scientific evidence. Alcohol kills us in the tens of thousands, while cannabis has no record of killing anyone.
Alcohol is a public menace while it remains legal and cannabis has no bad or deadly threats and is treated as a top-level killer, according to the idiotic DEA, which is one to the most useless federal government agencies. The drug war is over. We lost.
Originally posted by: http://lubbockonline.com/editorial-letters/2016-09-17/letter-cannabis-not-danger-its-said-be#


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Hello! I have found some cool stuff on Jordin Sparks, current as of 2019-10-05. I personally have always appreciated Jordin Sparks, so was eager to do some deep research into them. Let's get started!
First… how popular is Jordin Sparks right now? On Google Trends Jordin Sparks had a popularity ranking of 47 ten days ago, 56 nine days ago, 52 eight days ago, 60 seven days ago, 68 six days ago, 56 five days ago, 65 four days ago, 29 three days ago, 29 two days ago, 35 one day ago and now has a popularity rank of 36. So in the recent past, they were gathering the most attention on 2019-09-27 when they had a rank of 68. If we compare Jordin Sparks's popularity to three months ago, they had an average popularity of 57.5, whereas now their average popularity over the last ten days is 50.4. so by that measure, Jordin Sparks has regressed. It's worth noting, finally, that Jordin Sparks never had a rank of 0, indicating people are always searching for them 🙂
And what about how Jordin Sparks has fared if we consider the entire past 3 months? Our date indicates 2019-08-14 to be their most popular day, when they had a relative rank of 100. Not bad!
As of 2019-10-05, my research indicates that people searching for Jordin Sparks are also searching for these related terms: no air, jordin sparks no air, jason derulo jordin sparks, jordin sparks tattoo, jason derulo, jordin sparks songs, battlefield jordin sparks, battlefield, no air jordin sparks lyrics, jordan sparks, tattoo jordin sparks lyrics, jordin sparks net worth, jordin sparks battlefield lyrics, jordin sparks baby, jason derulo and jordin sparks, jordin sparks love will, jordyn woods, sage the gemini, jordin sparks wiki and who is jordin sparks married to.
I did some more thorough analysis today on the web sentiment regarding Jordin Sparks, and found a number news articles about them in the past month. I may update this post later when I analyze the best ones
Considering everything written about Jordin Sparks (not just official news articles), I decided the overall sentiment for Jordin Sparks on 2019-10-05 is neutral. But what do you think? I'd like to hear your comments (just don't write anything too mean).


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1/4 cup black or golden raisins
3 Persian cucumbers (about 1/2 pound)
24 ounces Greek yogurt or labneh (3 cups)
2 tablespoons any combination of finely chopped fresh parsley, cilantro, basil, tarragon or dill
1 garlic clove, finely grated or pounded into a smooth paste with a pinch of salt
Fine sea salt and freshly ground black pepper
3/4 teaspoon ground or crumbled dried rose petals (optional)
1 teaspoon dried mint
1 teaspoon dried dill
3 tablespoons roughly chopped toasted walnut pieces
In a small bowl, submerge the raisins in boiling water to plump for 10 minutes, then drain well.
Remove alternating stripes of peel on cucumbers and trim ends. Dice cucumbers into 1/4-inch pieces and place in a large bowl with raisins, yogurt, fresh herbs, garlic, 3/4 teaspoon salt, 1/2 teaspoon pepper and, if using, half the rose petals. Using your fingers to break up any large pieces, gently grind the dried mint and dried dill into the bowl. Stir to combine and adjust seasoning with salt as needed.
Just before serving, stir in the walnuts and transfer to serving bowl. Garnish with remaining rose petals, if using. Cover and refrigerate leftovers for up to 3 days.


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Austin Seferian-Jenkins
Last Updated: 2019-04-11
Top Stories about
Austin Seferian-Jenkins
179Austin Seferian-Jenkins - Wikipedia
131Austin Seferian-Jenkins Stats, News, Bio | ESPN
74Austin Seferian-Jenkins succinctly explains why hes joining the Patriots - Patriots Wire
73Austin Seferian-Jenkins on joining Patriots: 'I'm ready to ...
69Patriots sign tight end Austin Seferian-Jenkins - NBCSports.com
50Patriots Turn To Austin Seferian-Jenkins With Rob Gronkowski Gone - Forbes
48Patriots, TE Austin Seferian-Jenkins agree to one-year contract - Yahoo Entertainment
41Report: Patriots hosting Iowa TE Noah Fant for pre-draft visit - Patriots Wire
39NFL notebook: Giants WR Shepard gets hefty extension - National Football Post
34Austin Seferian-Jenkins Stats | Pro-Football-Reference.com
31Patriots sign TE Austin Seferian-Jenkins, report says ...
30Patriots Austin Seferian-Jenkins: Joining Patriots post-Gronk - CBSSports.com
29Austin Seferian-Jenkins, TE for the Jacksonville Jaguars ...
24Patriots sign TE Austin Seferian-Jenkins - UPI.com
21Austin Seferian-Jenkins seems thrilled to play with Tom ...
153 players the New England Patriots should still target in free agency - ClutchPoints
14REPORT: Former Jags RB T.J. Yeldon visited the Patriots Wednesday - Jaguars Wire
12Austin Seferian-Jenkins is Projected to Have a Better Fantasy Season in 2019 - Scout
11New England Patriots news 4/11/19 - - Pats Pulpit
10Patriots re-sign Gostkowski, add TE Seferian-Jenkins - Bangor Daily News
9Zolak & Bertrand: Kyries Comments, Brad Stevens, Bradys Instagram Post, Austin Seferian-Jenkins (H
8Patriots sign TE Austin Seferian-Jenkins to 1-year deal ...
7NFL rumors: Patriots may be looking to sign playmaker - Sporting News
6Patriots Talk: Patriots Draft Needs Following Free Agency - Trifecta Network
3Morning Roundup: Aces pick Jackie Young No. 1 in WNBA Draft - NewsOK.com
2Austin Seferian-Jenkins signing helps reduce urgency for Patriots - NBC Sports - Misc.
1NFL notebook: Giants wide receiver Shepard gets hefty extension - WSAU News
0New England Patriots Tom Brady joins Twitter, immediately fakes retirement - UPI News
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text: Global National January 24 2020 8:21pm 01:54 Raptors' Siakam named starter in his first NBA All-Star Game Toronto Raptor Pascal Siakam has been named a starter in his first-ever NBA All-Star Game. As Mike Drolet explains, in just four years, Siakam went from a NBA nobody to an NBA all-star. Rick Zamperin: NBA All-Star Pascal Siakam has only scratched the surface of his potential Toronto Raptors' Pascal Siakam named 2020 NBA all-star game starter Responsive site?


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The susceptibility of 30 clinical isolates belonging to six different species of filamentous fungi (Aspergillus fumigatus, Aspergillus flavus, Scedosporium prolificans, Scedosporium apiospermum, Fusarium solani, and Fusarium oxysporum) was tested against six antifungal drugs (miconazole, voriconazole, itraconazole, UR9825, terbinafine, and amphotericin B) with the microdilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) (M38-P). The MICs were compared with the MICs obtained by a colorimetric method measuring the reduction of the dye 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) to formazan by viable fungi. The levels of agreement between the two methods were 96 and 92% for MIC-0 (clear wells) and MIC-1 (75% growth reduction), respectively. The levels of agreement were always higher for Aspergillus spp. (97% ± 2.5%), followed byScedosporium spp. (87% ± 10.3%) and Fusariumspp. (78% ± 7.8%). The NCCLS method was more reproducible than the MTT method: 98 versus 95% for MIC-0 and 97 versus 90% for MIC-1. However, the percentage of hyphal growth as determined visually by the NCCLS method showed several discrepancies when they were compared with the percentages of MTT reduction. A new simplified assay that incorporates the dye MTT with the initial inoculum and in which the fungi are incubated with the dye for 48 h or more was developed, showing comparable levels of agreement and reproducibility with the other two methods. Furthermore, the new assay was easier to perform and more sensitive than the MTT method.
Filamentous fungi may cause invasive mycoses in both immunocompromised and nonimmunocompromised individuals. These infections require prompt systemic antifungal therapy, the effectiveness of which depends, among other things, on the in vitro susceptibility of the fungus to antifungal drugs. Therefore, there is a demand for reproducible and reliable methods of antifungal susceptibility testing of filamentous fungi. Many variables that decrease the inter- and intralaboratory agreement are now defined (2, 4, 5, 16, 19). Broth macrodilution methods are time-consuming and labor-intensive (1), and therefore, efforts have focused on microdilution methods that are more practical and more user-friendly in the clinical laboratory (18). Based on these principles, the Subcommittee on Antifungal Susceptibility Testing of the National Committee for Clinical Laboratory Standards (NCCLS) has proposed the M38-P standard for the antifungal susceptibility testing of conidium-forming fungi (14).
The determination of MICs for filamentous fungi can be facilitated with a method which overcomes observer bias and quantifies the hyphal growth of molds. Because turbidity measurements and colony counts (9) are not useful in the case of filamentous fungi, colorimetric methods based on the measurement of metabolic activity may facilitate determination of the MIC. One of these is an assay using the dye 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). This yellow tetrazolium salt is cleaved by dehydrogenases inside mitochondria or in other cellular locations possessing dehydrogenase activity (7) to form its purple formazan derivative (11), which can be measured spectrophotometrically at 550 nm. MTT is cleaved by all living, metabolically active fungi independent of proliferation and irrespective of unicellular or multicellular growth and thus is a measure of metabolic activity.
A method based on MTT has been used for susceptibility testing of fungi. The MTT method demonstrated excellent agreement with the standard macrodilution method for the antifungal susceptibility testing of yeasts (1, 9). For filamentous fungi, the MTT method has been used for Aspergillus fumigatus, resulting in interpretable data (10). In order to overcome the shortcomings of visual MIC determination for filamentous fungi and to develop a reliable, subjective, and less variable method for the antifungal susceptibility testing of filamentous fungi, we evaluated the performance of a method using MTT and compared the results with those obtained with the proposed standard of the NCCLS. The correlation between the metabolic activity and the growth of fungi was examined. Furthermore, a simplified method using MTT was developed and compared with the previous methods.
Test isolates.Thirty clinical isolates of filamentous fungi (part of our private collection) were tested. These included five isolates of each of the following species: A. fumigatus (AZN7151, AZN7275, AZN8249, AZN8248, and AZN7272),Aspergillus flavus (AZN501, AZN6837, AZN6803, AZN6686, and AZN6578), Scedosporium prolificans (AZN7886, AZN7903, AZN7946, AZN7921, and AZN7895), Scedosporium apiospermum (AZN1252, AZN7309, AZN7110, AZN7111, and AZN6474),Fusarium solani (AZN729, AZN646, AZN2106, AZN7844, and AZN2784), and Fusarium oxysporum (AZN685, AZN441, AZN1859, AZN8263, and AZN1188). Candida parapsilosis (ATCC 22019) andCandida krusei (ATCC 6258) were used for quality controls.
Isolates were passaged twice at an interval of 5 to 7 days at 30 to 37°C. All isolates were tested three times on three different days. All the solutions were prepared ex novo with powders from the same lot. Conidia of the isolates were obtained from fresh cultures each time.
NCCLS method.A broth microdilution method was performed according to NCCLS guidelines (M38-P) (14). The drugs that were used included miconazole (Janssen Research Foundation, Beerse, Belgium), terbinafine (Novartis, Basel, Switzerland), itraconazole (Janssen Research Foundation), UR9825 (Ureach, Madrid, Spain), voriconazole (Pfizer Central Research, Sandwich, United Kingdom), and amphotericin B (Bristol-Myers Squibb, Woerden, The Netherlands). The drug dilutions were made in RPMI 1640 medium (withl-glutamine, without bicarbonate) (GIBCO BRL, Life Technologies, Woerden, The Netherlands) buffered to pH 7.0 with 0.165 M morpholinepropanesulfonic acid (MOPS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany). The final concentrations of the antifungal agents ranged from 0.015 to 16 mg of amphotericin B/liter, 0.06 to 64 mg of miconazole/liter, and 0.03 to 32 mg of itraconazole, terbinafine, UR9825, and voriconazole per liter.
The tests were performed in 96-well flat-bottom microtitration plates, which were kept at −70°C until the day of testing. Spores were collected with a cotton stick and suspended in sterile water. After the heavy particles were allowed to settle, the turbidity of the supernatants were measured spectrophotometrically (Spectronic 20D; Milton Roy, Rochester, N.Y.) at 530 nm and transmission was adjusted to 68 to 70% (Scedosporium spp. and Fusarium spp.) or to 80 to 82% (Aspergillus spp.). Each suspension was diluted 1:50 in RPMI 1640 to obtain two times the desired inoculum. The inoculum size was confirmed by plating serial dilutions on Sabouraud dextrose agar (SDA) plates, with final inocula ranging from 1 × 104 to 5 × 104 CFU/ml. After agitation, the plates were incubated at 37°C for 48 h (Aspergillus spp. and Fusarium spp.) and 72 h (Scedosporium spp.). The growth was assessed by visual observation with the aid of a concave mirror. Growth was scaled according to the NCCLS guidelines as follows: 4, no reduction in growth; 3, slight reduction in growth, or approximately 75% of the growth control; 2, prominent reduction in growth, or approximately 50% of the growth control; 1, slight growth, or approximately 25% of growth control; and 0, optically clear, or the absence of growth. MIC-1 and MIC-0 were determined to be the lowest concentrations of drug showing slight growth (25%) and absence of growth (100% visible-growth inhibition) compared with that of the drug-free control, respectively. For amphotericin B, only the MIC-0 was determined.
MTT method (MTT-3h).The MICs were also determined by a colorimetric method using the dye MTT (Sigma Chemical, St. Louis, Mo.). After the MICs were visually determined on each of the microtitration plates, 25 μl of RPMI 1640 containing 5 mg of MTT/ml was added to each well. Incubation was continued at 37°C for 3 h. The content of each well was removed (centrifugation is not required for filamentous fungi due their adherence to the wells), and 200 μl of isopropanol containing 5% 1 M HCl was added to extract the dye. After 30 min of incubation at room temperature and gentle agitation, the optical density (OD) was measured with a microtitration plate spectrophotometer (MS2 reader, Titertekplus; ICN Biomedical Ltd., B.V., Zoetermeer, The Netherlands) at 550 nm. The OD of the blank, which consisted of an uninoculated plate incubated together with the inoculated plates, was subtracted from the ODs of the inoculated plates. The percentage of MTT conversion to its formazan derivative for each well was calculated by comparing the OD at 550 nm (OD550) of the wells with that of the drug-free control based on the following equation: (A550 of wells that contained the drug/ A550 of the drug-free well) × 100%. MIC-0 and MIC-1 were considered to be the lowest concentrations of drug showing at least 95 and 75% reductions in the OD compared with that of the drug-free well, respectively.
Toxicity test.The dye MTT was tested for possible inhibitory effects on the growth of filamentous fungi. The isolates were incubated in RPMI 1640 with different concentrations of MTT ranging from 4 to 0.06 mg/ml, for 72 h at 37°C. A final inoculum of 104 CFU/ml was used. Growth inhibition was assessed by visual observation of the wells containing MTT and compared with the MTT-free wells. The level of inhibition of the fungal growth was assessed and scored as described above.
Modified MTT method.The MICs for each of the isolates were also determined by a modification of the MTT method previously described. For this method, conidia suspensions were prepared as described above. Each suspension was diluted 1:50 in RPMI 1640 containing 0.2 mg of MTT/ml to obtain two times the desired inoculum. Then, the inoculum that contained MTT was added to microtitration plates, which resulted in a final concentration of MTT of 0.1 mg/ml, and the microtitration plates were incubated for 72 h (Scedosporium spp.) and 48 h (other species). After incubation, the formed formazan was extracted and the MICs were determined as described above.
Analysis of results.Each of the 30 isolates was tested three times by the three methods against the six drugs. For each isolate, there was one reading per test. The MICs derived from the three readings by the NCCLS method and the two MTT methods were compared for each drug-isolate combination.
(i) Agreement between NCCLS and MTT methods.The percentage of agreement between the NCCLS method and the MTT methods was defined as the proportion of MIC-0 and MIC-1 determined by MTT methods which fell within 1 dilution of the MIC-0 and MIC-1 determined by the NCCLS method, respectively, for each reading. The high and low off-scale MICs were included in the analysis by converting to the next higher or lower drug concentration, respectively. The differences were analyzed by two-way analysis of variance and were considered not statistically significant when the probability (P) exceeded 0.05.
(ii) Correlation of visual reading of growth with OD reduction.The correlation of the visual reading of growth with the OD reduction was estimated for each MIC endpoint by comparing the MIC-0 and MIC-1, as these were determined visually by the NCCLS method and spectrophotometrically by the MTT method (MTT-3h). The frequency of visual MICs, which fulfilled the criteria defined above for the MIC endpoints of the MTT method (at least 95 and 75% reductions of the OD for MIC-0 and MIC-1, respectively) as well as the average of percentage of OD reduction compared with the visual MIC endpoints were calculated.
(iii) Reproducibility of methods.For each method, the median of the three MICs measured was calculated and the proportion of the MICs that fell within 1 dilution of the median was estimated. Based on these calculations, the reproducibility of each method for determining the drug-MIC endpoint was determined.
A total of 1,620 MICs obtained by the three methods were evaluated in this study. All the isolates produced detectable growth at 48 and 72 h. The MICs of quality control strains were within the reference ranges for the three replicates.
MIC data.The MIC ranges for both MIC endpoints obtained by the NCCLS method for six antifungal drugs against the six fungal species are summarized in Table 1. Table1 also provides the median MIC of the three readings. In most of the endpoint-drug-species combinations, the MIC range and the median MIC were the same for the two methods, with very few exceptions with theFusarium spp. The MICs were distributed over all the drug concentrations used.
Toxicity of MTT.MTT did not have any effect on the growth of the fungi at concentrations of 0.125 mg/ml and lower (Fig.1). At the higher concentrations, MTT was toxic for Scedosporium spp. and Fusarium spp. since the growth was completely inhibited. By contrast,Aspergillus spp. appeared resistant to MTT even at higher concentrations.
Agreement between methods.The percentages of agreement within 1 dilution between MICs obtained by the NCCLS and MTT methods for each species-drug-MIC endpoint combination are presented in Table2. The results were stratified by antifungal agents and MIC endpoint. High levels of agreement (96% for MIC-0 and 92% for MIC-1) were found for the comparison of the NCCLS method with the MTT method. Lower levels of agreement were found when results of the modified MTT method were compared with those of the NCCLS method and the MTT method (MTT-3h). The agreement for MIC-0 was always higher than that for MIC-1. The observed differences among the methods were not statistically significant except for those derived from the comparison of the modified MTT method with the other two methods for both MIC endpoints of voriconazole and for MIC-1 of UR9825.
The levels of agreement for each drug-MIC endpoint combination between the methods were always the highest for the Aspergillus spp. (97% ± 2.5%), followed by Scedosporium spp. (87% ± 10.3%). For Fusarium spp., the levels of agreement were relatively low (78% ± 7.8%) (data not shown).
Representative curves of the OD and percentage of MTT conversion of miconazole against S. apiospermum are presented in Fig.2. MIC-0 and MIC-1 determined visually by the NCCLS method were 1 and 0.5 mg/liter, respectively.
Correlation between visual reading of growth and OD reduction.Visual reading of growth was correlated with the colorimetric assessment of metabolic activity of fungi by comparing the results of the NCCLS method with the results of the MTT method (MTT-3h) (Table3). The off-scale MICs were excluded from the analysis. For the MIC-0 endpoint, 84.7% of the MIC-0s of all drugs determined by the NCCLS method corresponded to a 95% or greater reduction of the OD. For the MIC-1 endpoint, 63.9% of the MIC-1s obtained by the NCCLS method corresponded to a 75% or greater reduction of the OD.
Reproducibility of methods.Overall, the three methods were very reproducible, since the levels of the reproducibility were higher than 89% for both MIC endpoints. The NCCLS method was more reproducible than the MTT methods, since the reproducibility for MIC-0 and MIC-1 were 94.8 and 93.4% for the NCCLS method compared with 94.8 and 89.5% for the MTT method and 92.8 and 89.6% for the modified MTT method, respectively. Between the two MIC endpoints, MIC-0 was more reproducible than MIC-1. For all drugs tested, the reproducibility of MIC-0 ranged from 90 to 100% and that of MIC-1 ranged from 82 to 99%. The lowest reproducibility among the drug-MIC endpoint-method combinations was exhibited by MIC-1 of terbinafine determined by the MTT method and the modified MTT method (82 and 84%, respectively).
There are several problems in the determination of the MICs of antifungal agents, such as trailing effect caused by partial inhibition of fungal growth (8) and the subjectivity of visual reading with the aid of a magnifying mirror (16). These shortcomings are increased for filamentous fungi, which produce hyphae, making quantification of growth difficult. Moreover, the unequal growth, clumping, and adherence (13) of molds in assay tubes makes establishing a clear endpoint impossible both by visual reading as well as by spectrophotometer.
The colorimetric method using the dye MTT has been investigated as an alternative method for the NCCLS method for in vitro susceptibility testing of fungi. Its applicability was investigated for the MIC of fluconazole against 101 yeast isolates, and high levels of agreement with the NCCLS method were reported (1). In the same study, the authors found that the MICs could be determined 24 h earlier with the MTT method. Although previous studies have shown that the MTT method resulted in interpretable dose-response curves with both amphotericin B and itraconazole against A. fumigatus(9, 10), the applicability of the MTT method for the antifungal susceptibility testing of various filamentous fungi against different antifungal drugs and the comparison of this method with the NCCLS method (M-38P) had not been studied.
Overall, the levels of agreement between the MTT and NCCLS methods within 1 dilution were 95.8% based on MIC-0 and 92.3% based on MIC-1 (Table 2). The MTT method relies on metabolic activity of mycelia (6, 11) and, thus, not directly on growth. Any factor which influences the metabolic rate might have an effect on reduction of MTT even if the biomass remains the same. The high levels of agreement with the NCCLS method might indicate that growth corresponds directly with the metabolic status of fungi. However, different levels of agreement were found between the genera, with Aspergillus spp. showing higher levels of agreement (97% ± 2.5%) than Scedosporium spp. (87% ± 10.3%) andFusarium spp. (78% ± 7.8%). This could be explained by the different rates and manner of sporulation and, if true, indicates potential difficulties in developing a general, accurate, comprehensive assay for testing the susceptibility of conidium-forming filamentous fungi in vitro.
Although the microtitration plates were prepared ex novo, the two methods showed high reproducibility since the levels of agreement among the replicates were 97.6 and 96.7% (NCCLS) and 94.8 and 89.5% (MTT) based on MIC-0 and MIC-1, respectively. The lower reproducibility of MIC-1 compared with that of MIC-0 in the MTT method could be explained by the high sensitivity of the assay. Different variables in the conditions of incubation, such as batch of medium, temperature, and evaporation, might have an effect in the reduction of MTT influencing the less-stable MIC-1 endpoint to a higher degree.
The correlation of the MIC endpoints in two methods showed some discrepancies since only 84.7% of the MIC-0s determined by the NCCLS method corresponded with metabolic activity lower than 5%, as assessed by MTT conversion, and 63.9% of visually determined MIC-1s by the NCCLS method corresponded to MTT conversion lower than 25% (Table 3). The discrepancies that occurred from the comparison of MIC endpoints were expected since the accurate method of MTT precisely quantifies the growth in each well, which is very difficult to achieve by visual reading.
In order to increase the reduction of MTT and diminish the incubation period required for the MIC determination, it has been suggested to use menadione, an electron-coupling agent that increases the reduction of MTT (6, 9, 10). However, we were able to increase the reduction of MTT just by adding the dye to the inoculum and by incubating the fungi with the dye for 48 and 72 h. This resulted in a 1.5- to 5-fold increase of absorption due to the higher production of formazan (Fig. 2). To ensure that MTT does not inhibit the growth of fungi, we incubated the fungi with different concentrations of MTT. All fungi were inhibited by a high concentration of MTT, although the susceptibility varied among the species. No inhibition was observed at a concentration of 0.125 mg/ml or lower when all the isolates were incubated for 72 h with the dye (Fig. 1). Therefore, a final concentration of 0.1 mg of MTT/ml was used in the modified MTT assay. The agreement of this new assay with the NCCLS method was lower than that of the MTT method (86.6% for MIC-0 and 78.5% for MIC-1), probably due to interexperimental variability (different plates) and its higher sensitivity, since small differences in the growth rate can result in large differences in the amount of formazan that is produced. The discrepancies for UR9825 and voriconazole were statistically significant. This could be related to the trailing effect, which might influence the results obtained by the modified MTT method more than those derived by the MTT assay. However, the modified assay was very reproducible (92.8% for MIC-0 and 89.6% for MIC-1), compared to the MTT method. The MTT method incorporates several advantages. It is easy to perform and objective (1). The MICs of antifungal drugs can be determined rapidly since it can produce results after 24 h for Aspergillus spp. and 48 h forScedosporium spp., although the reduction of MTT increased during prolonged incubation (unpublished observations). The MTT method is reproducible and reliable. It provides good agreement with the NCCLS method while overcoming some of its drawbacks. The main advantage of the MTT method is that it enables precise quantification of hyphal proliferation of molds and can assess even very small differences. This property is very important in cases that interactions between combinations of antifungal agents are studied (12).
A drawback of the MTT method is the requirement of high numbers of cells, especially if they have low metabolic activity (6), in order to achieve measurable MTT reduction. Furthermore, the high sensitivity can decrease the reproducibility in some experimental designs. The extraction of formazan crystals is performed by dissolution in organic solvents (15), which precludes direct spectrophotometric measurement of absorption (20) and prevents the assessment of fungicidal concentration of the drugs. Furthermore, an additional extraction step is required to visualize the reduction of MTT (20). Alternatively, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) could be considered an alternative since it is reduced to a water-soluble formazan product (8, 20; J. Meletiadis, unpublished observations).
In conclusion, the MTT method can be used as an alternative method to the NCCLS method since it shows high levels of agreement with the NCCLS method. The spectrophotometric reading provides more detailed information on the antifungal activity in each well. Furthermore, optimalization of the MTT method may overcome the above-mentioned drawbacks. The modified assay we described in this study could be the starting point for further optimization of the MTT method.
This study was supported by the EC-TMR-EUROFUNG network (ERBFMXR-CT970145) and by the Mycology Research Center of Nijmegen.
- Received 6 March 2000.
- Returned for modification 16 May 2000.
- Accepted 3 June 2000.
- Copyright © 2000 American Society for Microbiology


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Journaled News-Updates | BSD Now 22
Posted on: January 30, 2014
Posted in: BSD Now, Featured, Video
We talk with George Neville-Neil about the brand new FreeBSD Journal and what it\'s all about. After that, we\'ve got a tutorial on how to track the -stable and -current branches of OpenBSD. Answers to all your BSD questions and the latest headlines, only on BSD Now – the place to B.. SD.
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– Show Notes: –
FreeBSD quarterly status report
- Gabor Pali sent out the October-December 2013 status report to get everyone up to date on what\'s going on
- The report contains 37 entries and is very very long… various reports from all the different teams under the FreeBSD umbrella, probably too many to even list in the show notes
- Lots of work going on in the ARM world, EC2/Xen and Google Compute Engine are also improving
- Secure boot support hopefully coming by mid-year
- There\'s quite a bit going on in the FreeBSD world, many projects happening at the same time
- Jordan (jkh), one of the co-founders of the FreeBSD project, is once again a FreeBSD committer
n2k14 OpenBSD Hackathon Report
- Recently, OpenBSD held one of their hackathons in New Zealand
- 15 developers gathered there to sit in a room and write code for a few days
- Philip Guenther brings back a nice report of the event
- If you\'ve been watching the -current CVS logs, you\'ve seen the flood of commits just from this event alone
- Fixes with threading, Linux compat, ACPI, and various other things – some will make it into 5.5 and others need more testing
- Another report from Theo details his work
- Updates to the random subsystem, some work-in-progress pf fixes, suspend/resume fixes and more signing stuff
Four new NetBSD releases
- NetBSD released versions 6.1.3, 6.0.4, 5.2.2 and 5.1.4
- These updates include lots of bug fixes and some security updates, not focused on new features
- You can upgrade depending on what branch you\'re currently on
- Confused about the different branches? See this graph.
The future of open source ZFS development
- On February 11, 2014, Matt Ahrens will be giving a presentation about ZFS
- The talk will be about the future of ZFS and the open source development since Oracle closed the code
- It\'s in San Jose, California – go if you can!
This episode was brought to you by
Interview – George Neville-Neil – email@example.com / @gvnn3
Tracking -STABLE and -CURRENT (OpenBSD)
News Roundup
pfSense news and 2.1.1 snapshots
- pfSense has some snapshots available for the upcoming 2.1.1 release
- They include FreeBSD security fixes as well as some other updates
- There are recordings posted of some of the previous hangouts
- Unfortunately they\'re only for subscribers, so you\'ll have to wait until next month when we have Chris on the show to talk about pfSense!
FreeBSD on Google Compute Engine
- Recently we mentioned some posts about getting OpenBSD to run on GCE, here\'s the FreeBSD version
- Nice big fat warning: \"The team has put together a best-effort posting that will get most, if not all, of you up and running. That being said, we need to remind you that FreeBSD is being supported on Google Compute Engine by the community. The instructions are being provided as-is and without warranty.\"
- Their instructions are a little too Linuxy (assuming wget, etc.) for our taste, someone should probably get it updated!
- Other than that it\'s a pretty good set of instructions on how to get up and running
Dragonfly ACPI update
- Sascha Wildner committed some new ACPI code
- There\'s also a \"heads up\" to update your BIOS if you experience problems
- Check the mailing list post for all the details
PCBSD weekly digest
- 10.0-RC4 users need to upgrade all their packages for 10.0-RC5
- Help test GNOME 3 so we can get it in the official ports tree
- By the way, PCBSD 10.0 is out!
- Special thanks to developers, testers, translators and docs team!
- Upcoming: Working on a 11-CURRENT PC-BSD and 10-STABLE
- Tony writes in: http://slexy.org/view/s21ZlfOdTt
- Jeff writes in: http://slexy.org/view/s2BFZ68Na5
- Remy writes in: http://slexy.org/view/s20epArsQI
- Nils writes in: http://slexy.org/view/s213CoNvLt
- Solomon writes in: http://slexy.org/view/s21XWnThNS
- All the tutorials are posted in their entirety at bsdnow.tv
- Send questions, comments, show ideas/topics, or stories you want mentioned on the show to email@example.com
- Watch live Wednesdays at 2:00PM Eastern (19:00 UTC)
- A BSD Now t-shirt design is in the works, we\'ll update you on the progress (but we have to get permission to use the mascots and get a rough sketch first)
- NYCBSDCon will be on February 8th in NYC
- We\'ll announce the winner of our tutorial contest on next week\'s episode! Get your last minute tutorial submissions in for our contest


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Job Information
Anthem, Inc. IT Change Management Support Internship- PS30724 in East Syracuse, New York
IT Change Management Support Internship- PS30724
Location: United States
Requisition #: PS30724
Post Date: Mar 09, 2020
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Support technical projects spanning Enterprise Applications such as Remedy, SailPoint, SharePoint, to applications that support our Medicare Programs such as Contact Center, Case Management, and Data Warehouse/Business Intelligence.
Primary duties may include, but are not limited to:
Coordinates and responds to requests for change and administers these requests within the Change Management System (Remedy).
Facilitates and/or participates in weekly change management and status meetings.
Monitors changes for cross functional dependencies and ensures that all Change Management policies are followed with each change that is submitted.
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Be currently enrolled in a program of study at an accredited college or university working on undergraduate studies
Possess strong written and oral communication skills
Be proficient in MS Office applications like MS-Word and Excel; being familiar with SharePoint is a plus
Has ability to commute to our office on the northeast corner (Castleton area) of Indianapolis
Anthem, Inc. is ranked as one of America's Most Admired Companies among health insurers by Fortune magazine and is a 2018 DiversityInc magazine Top 50 Company for Diversity. To learn more about our company and apply, please visit us at careers.antheminc.com. An Equal Opportunity Employer/Disability/Veteran.
Anthem, Inc.
- Anthem, Inc. Jobs


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Plates, Dishes, Basins & Chargers
Teapots, Coffeepots, Pitchers & Sugar Bowls
Mugs, Tankards & Measures
Church Pewter
Iron for the Hearth
Other Items
Civil War
Tel: (860) 267-2146
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American Pewter > Plates, Dishes, Basins & Chargers
A very fine condition 12 1/8"" American Pewter platter by Jacob Whitmore, Middletown, Connecticut 1757-1790. Jacob Whitmore had an association with Thomas Danforth II, of Middletown (1755-1782) in that they worked together and jointly owned a number of molds for casting pewter.
Jacob is known to have produced plates in various sizes basins and mugs both pint and quart sizes. This 12" plate is the finest example we have ever had. It shows very little use. The face surface retains most of the original lathe marks. The touches are fine "as struck". Only the back of the plate has some light etching with some fine pitting. A superb example
We are only open by appointment. tel (860) 267-2146
Please call between 9:00 AM-8:00 PM EST
© Wayne Hilt, 2020


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2590 Buttermilk Pike VillaHills KY
Great opportunity in Villa Hills with a full brick ranch cared for by former owners who are relocating. Super large eat-in kitchen w/updated stainless steel appliances and counter island w/extra storage. Beautiful dark maple cabinetry and Corian counter top. Lots of real hardwood. Great flat, fenced rear yard. Full bathrooms on main floor and lower level. Super convenient to shopping & interstate system. Entire basement was water proofed by Ever-Dry with warranty. Come visit with your realtor or visit our open house. Verify schools.
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Phone: 513-478-4318
The data relating to the real estate for sale on this web site comes in part from the Broker Reciprocity program of the Northern Kentucky Multiple Listing Service, Inc. Real estate listings held by brokerage firms other than Comey & Shepherd Realtors are marked with the Broker Reciprocity logo or Broker Reciprocity house icon. The properties displayed may not be all the properties available through Broker Reciprocity.


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Hooded Trench Coat
angled pockets, button strap cuffs, epaulettes, back vent, detachable hood, removable belt,hits at knee,lightweight,hooded, double breasted button front and belt closure,model's height is 5ft. 10in., wearing size s,cotton/polyester,imported
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3421 Tittabawassee Rd
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Instead of turning to your kid's heap of sugar-laden Halloween treats, we have a tasty alternative for satiating your sweet tooth: SmartSweets. These gummy candies have 85 percent less sugar than the average candy. In fact, one entire bag of SmartSweets fun-pack gummy candy has only one gram of sugar (most others have around eight grams!)
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Paper copies of past examination papers are not held by the University Centre Library, but downloadable PDFs and Word documents of the University Centre at Blackburn College's past papers are available online from the Past Examination Papers Database.
Past papers are uploaded by Course to the database and can be downloaded. The earliest papers are available from 2018/19.
No model answers are provided with the papers .
If you discover a problem with any of the associated papers, for example the file will not download, please send an email to the University Centre Library stating the exam paper Module Code and the Title to :


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As Floridians we are all familiar with the saying, "it's so hot outside you could fry an egg." Our summer days can reach temperatures well over 90 degrees making daily outdoor exercise miserable for us and our four-legged companions. Some owners believe dogs can tolerate the heat. The truth is if you're hot, they are hot too. Our Tampa Bay veterinarian would like to share a few tips on keeping your canine cool and protected during these dog days of summer.
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- Avoid Pavement: When possible stay on grassy or dirt trails. These will always be much cooler then hard paved surfaces.
- Surface Test: If paved areas are the only option, then test all surfaces before venturing out. If you can comfortably hold your hand, palm down on the surface for 10 seconds the pavement is safe to walk on.
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In the end we all want what is best for our pets when it comes to their health. Although exercise is extremely important so is ensuring their overall well-being. Along with the tips above and the helpful reminder, if you're hot they're hot, you are set to protect your pet during the scorching summer months. If you have questions regarding your pet's exercise routine, the summer heat, or any other health related issue please contact our hospital today at 813-501-4985. One of our team members is ready to serve you.


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DEF/LIGHT - Transcendevil (CD)
$ 20.57
$ 20.99
Release Date: 04/15/14
Second full-length album by Ukrainian Black/Death Metal band for fans of: Dimmu Borgir, Hate, Behemoth etc.
The new work of Ukrainian Black/Death Metal band is a brutal, inspired blast of darkness. Symphonic elements emphasize the aggressiveness of the compositions in the album, and the fully symphonic track 'Septem Million' is a real step forward in the development of the band. Recommended for fans of: Dimmu Borgir, Hate, Behemoth, Flashgod Apocalypse, Anorexia Nevrosa.
|Label: Metal Scrap Records
|Genre: Metal
|Language: English
|Run Time: 49:14 mins
NOTE: International orders are shipped using FedEx International Mail Service. Delivery can take up to 8 weeks and no tracking number is provided by this service. This is done to reduce shipping costs. If you require faster shipping or shipping with tracking, please contact us for a shipping cost.


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If you require a fast and dependable team to change your stunning PSD to HTML5 conversion facilities then, search no more. Global Niche Tech offers an extremely experienced front-end advancement group which will effortlessly change your PSD documents to culminate astounding, cross-program good W3C Valid HTML.
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Avail Best PSD to HTML Services For Your Desirable Template
Furthermore, you will avail the best PSD to HTML service to established your desirable, and unique templates. We also help you to design quick and easy layouts and we won`t disappoint you too.[read more="Read More" less="Read Less"] We always make sure to deliver you the best and perfect design templates, so that your brand can achieve the maximum amount of traffic to your website on a daily basis. As we all are living in a world which is full of art and technology that's why it is now becoming more well-known in IT business. Being in a highly competitive high-tech industry, we understand what you need. So get our brilliant PSD to html5 conversion services so that you can save your money and time both at the same time.