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Create an Account - Increase your productivity, customize your experience, and engage in information you care about.
The Hall of Justice hours have been changed to 9:00am to 3:00pm daily. District Court will still be open 8:30am – 5:00pm and can be reached by phone at 360-577-3073.
Weddings performed by a District Court Judge have also been suspended until further notice.
Cowlitz County District Court is a court of limited jurisdiction. The court hears criminal, civil, traffic infraction, and miscellaneous cases filed by:
In addition to these cases the cities of Longview, Kelso, Kalama, Woodland, and Castle Rock contract with the District Court to have their cases heard.
District Court clerks are not allowed to give you any legal advice. This includes: what to say in court, how to file civil forms, what plea to make on a charge, or change any order from a judge.
District Court judges are elected to four-year terms and, in addition to hearing cases, they are authorized to perform civil marriage ceremonies.
Contact the Court


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subset: commoncrawl


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Super cute pair of black and white kitten salt and pepper shakers in a basket. These kittens in a basket were made in Japan for Takahashi of San Francisco, an importer of interesting and quality goods for their store in San Francisco. Wonderful details on the cats with mischievous expressions and blue eyes. Perfect gift for the cat lover.
CONDITION: Excellent with no chips, cracks, or nicks. Plastic corks.
SIZE: 4 1/4 inches long, 3 inches tall.
You can view my other vintage shakers here: https://www.etsy.com/shop/BagBagSydVintage?ref=l2-shopheader-name§ion_id=19334678
SHAKERS: I lightly clean all my vintage salt and pepper shakers - however they are vintage, and some have been used over the course of their lifetime and may contain residue of salt or pepper. I try to eliminate as much of that as possible, but these items are often fragile in their decorations - most novelty shakers were not actually meant to be used so cannot be soaked or scrubbed. With original corks I leave them in, as to try and remove then could destroy them.
1-3 business days
I'll do my best to meet these shipping estimates, but can't guarantee them. Actual delivery time will depend on the shipping method you choose.
Buyers are responsible for any customs and import taxes that may apply. I'm not responsible for delays due to customs.
Just contact me within: 14 days of delivery
Ship items back to me within: 30 days of delivery
Request a cancellation within: 2 days of purchase
But please contact me if you have any problems with your order.
Because of the nature of these items, unless they arrive damaged or defective, I can't accept returns for:
Buyers are responsible for return shipping costs. If the item is not returned in its original condition, the buyer is responsible for any loss in value.
I do not keep any private information on my buyers, nor do I contact them after the transaction is completed, except on Etsy when requested by the buyer. I only use name and addresses to fulfill the order. I have no control over what third party mailing services do with the info.


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"La Tortura (With Alejandro Sanz)"
check it out: on Vh1
Something about it and Alejandro is cute!
"La Tortura (With Alejandro Sanz)"
check it out: on Vh1
Something about it and Alejandro is cute!
i luv the male singas part
but y she dance so hard
she's giving taina a run for da La Salvaje money...
It looks like !!Originally Posted by PtHova
Elq- I'm sure Taina can give her a ticket to where ever she came from!! RIGHT TAI TAI!! lol
I was just looking at that video and about to post it . all I can say is Mmmmmmmmmm
the song is hot,.. i love them both,...i think alejandro has a sexy ass voice,....and shakira is just the bomb all around
my sentiments exactlyOriginally Posted by WadadliEmpress
Originally Posted by DRAGON
are you watchin VH1?? Because I'm LOVIN COLDPLAY's new video...
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of this woman nice video
empress he's a hottie
My friend did it... AND SHE LOVED IT!! do it!Originally Posted by trini_chic
She said that she's shaken every other part of her body so she decided to try something new - her chest.Originally Posted by PtHova
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myCode – improving care together with young adults with cancer
How can we improve care and life for teenagers and young adults with cancer? The answer to this question is being explored by the Young Cancer support association and Karolinska University Hospital through the joint innovation project myCode, supported by several other stakeholders.
The goal is to enhance quality of life and increase survival by focusing on young people under treatment – and on new technology to improve communication between patients and the healthcare system.
The teen years can be a difficult period for anyone. This challenge becomes considerably more difficult for young people who at the same time mus cope with a cancer diagnosis and the emotional, aesthetic and health-related side effects that accompany its treatment.
We see a need to recognize teenagers and young adults (15-29 years) with cancer as a special group:
- 50 percent report psychiatric and psychosocial problems, including post-traumatic stress syndrome, anxiety and depression.
- The disease also impedes opportunities for education, employment and self-sufficiency.
- Research also shows that survival is worse compared with children and adults with the same disease. In certain groups, the difference is as high as 30 percent. There is a link here between inadequate understanding of – and therefore adherence to – the treatment.
New technology and venues
"We would like the various needs of individuals to guide development in order to create a holistic view in which the patient is actively engaged," says Therese Sjöberg, who is head of the innovation project.
95% have already discussed their disease on social media.
"For example, how can we create attractive digital social environments for encounters, dialogue and support adapted to this target group, which is well-acquainted with social media – and where 95 percent have already talked about their disease on Facebook?"
Large group in need of a place to belong
Young people are one of the larger age groups, who currently sense a lack of belonging in the healthcare system and do not always feel at home in either the pediatric or the adult wards. Therefor we are now exploring new innovative solutions to meet the specific needs of teenagers and young adults – regarding both access to care and the design of technological solutions.
The idea is to ultimately provide new venues for the encounter between health care and young patients – or between friends. The project will also explore whether virtual encounters, where patients in a similar situation meet one another, can be effective.
In Sweden, 800 teenagers and young adults (15-29 years) are diagnosed with cancer annually. Worldwide, the corresponding figure is 1.5 million new cases per year.
FILM. How to encounter young cancer patients
Teenagers and young adults with cancer often have different needs than children and senior patients. This is a fact resulting in not only a sense of not feeling at home at the ward, but also effects wellbeing and survival rates. An innovation project at Karolinska University Hospital healthcare aims at improving healthcare for this patient group – through a collaboration between patients, healthcare staff and a multitude of other stakeholders representing industry and society. The project is led by the Center for Innovation at the hospital and the cancer care wards in close collaboration with the patient organization Young Cancer.
myCode – joint innovation to improve cancer care for teenagers and young adults
The innovation project is exploring how healthcar can change and create new – maybe digital – venues, in order to facilitate for young patients to follow their treatment plan and get healhtcare customized for their specific needs. The overall goal is to increase wellbeing and survival rates.
myCode stnads for my solution to effect my life as a patient, according to my needs with technology supporting me. The name of the project follows the ambition to
Parties: Karolinska Universitety Hospital och Young Cancer support organization Cancer in collaboration with Childhood Cancer Foundation, Regional Cnacer Centers, Swedish Association of Local Authorities and Regions and industry partners. Financial support from Region Stockholm Innovation, Childhood CAncer Foundation and Vinnova.
Therese Sjöberg, Innovation Leader
Phone: 072-719 65 58
Email: Therese Sjöberg


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How Bad Air Balancing Can Harm Your Commercial Building
Air balancing is crucial for a healthy commercial heating system. Air balancing helps to maintain climate control and ventilation. Buildings can often deal with spots of hot and cold air and areas with both high and low humidity. The following are some things you should know about air balancing in your commercial space.
What Is Air Balancing?
Air balancing helps to make sure the heating and air conditioning system delivers the necessary air and air pressure to a room. An HVAC technician assesses the airflow from different registers throughout a building to check the system pressure. The technician will also check the heat and humidity levels. These tests will tell you what issues your building has with the balance of air and what should be done to correct it.
Why Is Air Balancing Important?
A heating and cooling system does more than keep a room at a comfortable temperature. When the balance of air is not how it should be the system will be out of tune. You will ultimately spend more on energy costs to heat and cool a room without the temperature ever feeling right. You can experience drastic changes in humidity levels, exhaust buildup in rooms due to issues with air pressure, condensation buildup on your equipment, and overall discomfort for those who are in your building.
What Is the Cause of Bad Air Balance?
There are several causes of bad air balance in a building. The issues can stem from leaky ductwork, decreased power in the blower or exhaust fan, the wrong size of air filters, or broken or improperly installed outdoor air dampers.
The process of air balancing includes the identification of a specific problem. The HVAC technician will perform several diagnostic tests to determine the main cause of the issue, then proceed to either repair the problem or tell you what you can do to prevent poor air balance.
Repairing an air balancing issue is a delicate and complex process that only a professional HVAC technician should undertake. While you can do some general maintenance yourself, a professional diagnosis is necessary to get to the bottom of such an issue in a commercial building.
If you think you may have some problems with air balancing in your building, be sure to contact your HVAC contractor right away. The proper balance of hot and cold air will ensure you do not experience any expensive damages to your building or equipment due to the issues caused by poor air balance. Commercial air conditioning repair may be necessary.


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We used a maskless photolithography method to produce DNA oligonucleotide microarrays with unique probe sequences tiled throughout the genome of Drosophila melanogaster and across predicted splice junctions. RNA expression of protein coding and nonprotein coding sequences was determined for each major stage of the life cycle, including adult males and females. We detected transcriptional activity for 93% of annotated genes and RNA expression for 41% of the probes in intronic and intergenic sequences. Comparison to genome-wide RNA interference data and to gene annotations revealed distinguishable levels of expression for different classes of genes and higher levels of expression for genes with essential cellular functions. Differential splicing was observed in about 40% of predicted genes, and 5440 previously unknown splice forms were detected. Genes within conserved regions of synteny with D. pseudoobscura had highly correlated expression; these regions ranged in length from 10 to 900 kilobase pairs. The expressed intergenic and intronic sequences are more likely to be evolutionarily conserved than nonexpressed ones, and about 15% of them appear to be developmentally regulated. Our results provide a draft expression map for the entire nonrepetitive genome, which reveals a much more extensive and diverse set of expressed sequences than was previously predicted.
Characterization of the complete expressed set of RNA sequences is central to the functional interpretation of each genome. For almost 3 decades, the analysis of the Drosophila genome has served as an important model for studying the relationship between gene expression and development. In recent years, Drosophila provided the initial demonstration that DNA microarrays could be used to study gene expression during development (1), and subsequent large-scale studies of gene expression in this and other developmental model organisms have given new insights into how organisms implement their developmental plan (2). Additional studies in Drosophila have addressed questions in diverse biological processes, including metamorphosis, aging, innate immune response, and sexual dimorphism, and in evolutionary patterns of gene expression (3–9). The cumulative data from such microarray studies are valuable for functional annotation of the genome. However, all microarray studies of gene expression in Drosophila have been restricted to fragments of genes from predicted gene sets and thus are subject to the limitations of gene prediction algorithms. Large-scale expressed sequence tag (EST) sequencing has also been extremely valuable for annotation of the Drosophila genome (10), but this approach is limited by biases due to 5′ or 3′ end DNA sequencing, the extent to which transcripts are represented in cDNA libraries, and the number of EST sequences generated. A nonbiased approach is necessary for the determination of the entire catalog of expressed sequences in the genome. Genomic "tiling"DNA arrays, which use oligonucleotides or polymerase chain reaction (PCR) products corresponding to chromosomal sequences as probes, have been used to create transcript activity catalogs for portions of the human genome and for Arabidopsis (11–13). Microarrays have also recently been used to characterize the great diversity of RNA transcripts brought about by differential splicing in human tissues (14). We used both types of approaches to characterize the Drosophila genome.
Experimental design. To determine the expressed portion of the Drosophila genome, we designed high-density oligonucleotide microarrays with probes for each predicted exon and probes tiled throughout the predicted intronic and intergenic regions of the genome. We used maskless array synthesizer (MAS) technology (15, 16) to synthesize custom microarrays containing 179,972 unique 36-nucleotide (nt) probes (17). Of these, 61,371 exon probes (EPs) assayed 52,888 exons from 13,197 predicted genes, 87,814 nonexon probes (NEPs) assayed expression from intronic and intergenic regions, and 30,787 splice junction probes (SJPs) assayed potential exon junctions for a test subset of 3955 genes. For the SJPs, we used 36-nt probes spanning each predicted splice junction, with 18 nt corresponding to each exon (14). RNA from six developmental stages during the Drosophila life cycle (early embryos, late embryos, larvae, pupae, and male and female adults) was isolated and reverse-transcribed in the presence of oligodeothymidine and random hexamers, and the labeled cDNA was hybridized to these arrays. The stages were chosen to maximize the number of transcripts that would be differentially expressed between samples on the basis of previous results (3, 7). Each sample was hybridized four times, twice with Cy5 labeling and twice with Cy3 labeling (fig. S1).
Genomic and chromosomal expression patterns. We determined which exon or nonexon probes correspond to genomic regions that are transcribed at any stage during development (18). We used a negative control probe (NCP) distribution (fig. S3) to score the statistical significance of the EP or NEP signal intensities for each of the 24 unique combinations of stage, dye, and array, correcting for probe sequence bias (17, 19). These results were combined into a single expression-level estimate (19), a threshold for which was determined by requiring a false discovery rate of 5% (20). This threshold shows 47,419 of 61,371 EPs (77%) and 35,985 out of 87,814 NEPs (41%) were significantly expressed at some point during the fly life cycle. Significantly expressed EPs correspond to 79% (41,559/52,888) of all exons probed and 93% (12,305/13,197) of all probed gene annotations. Our results confirmed 2426 annotated genes not yet validated through an EST sequence (Fig. 1A). Out of 10,280 genes represented by EST sequences, only 401 (3.0%) were not detected in these microarray experiments. Our finding that a large fraction of intergenic and intronic regions (NEPs) is expressed in D. melanogaster mirrors similar observations for chromosomes 21 and 22 in humans (16) and for Arabidopsis (14). These results support the conclusion that extensive expression of intergenic and intronic sequences occurs in the major evolutionary lineages of animals (deuterostomes and protostomes) and in plants.
We noted that mRNA expression levels for protein-encoding genes varied with the protein function assigned in the Drosophila Gene Ontology (fig. S2) (21). For example, genes encoding G protein receptors were expressed at relatively low levels, whereas genes encoding ribosomal proteins were highly expressed. A gene's expression level was also associated with cellular compartmentalization and the biological process it mediates (fig. S2). For example, genes encoding cytosolic and cytoskeletal factors were more highly expressed than those predicted to function within organelles such as the endoplasmic reticulum, Golgi, and peroxisome. To determine whether a high level of gene expression was associated with essential genetic functions, we examined the expression levels of genes recently shown to be required for cell viability (Fig. 1B) in a genome-wide RNA interference (RNAi) screen in Drosophila (22). Compared to the rest of the genome, the genes identified as essential by RNAi showed a significant increase in expression during all stages of development (P = 0.0009, t test), even when the highly expressed ribosomal protein genes were omitted (P = 0.0005, t test). This result is also consistent with the observation that genes with mutant phenotypes from the 3-Mbase Adh genomic region are overrepresented in EST libraries (23). High levels of essential gene expression may in part reflect widespread expression in cells throughout the animal, and the relative RNA expression level may serve as a rough predictor of essential cellular function.
We also examined changes in gene expression during the fly life cycle to determine what fraction of the entire genome is differentially expressed between developmental stages. Figure 2A shows the expression signal intensities of transcripts from a typical 50–kilobase pair (kbp) region of the Drosophila genome during each major developmental stage. Stage-specific variation in expression is observed not only for exon probes, as expected, but also for intergenic and intronic probes. We used analysis of variance (ANOVA) (24) to systematically identify probes as differentially expressed at a false discovery rate of 5% (16). As expected, the majority of probes detecting differentially expressed sequences are also expressed above background noise level (89% of EPs and 81% of NEPs) (17) (Table 1). We found 27,176 EPs to be differentially expressed, corresponding to 76% of annotated genes, and even more when we applied a less conservative background model (fig. S4). The fact that the majority of genes are developmentally regulated is consistent with previous results obtained with cDNA microarrays (3). In intergenic and intronic regions, we detected differential expression for 15% (5508/35,985) of significantly expressed NEPs, indicating that many of the putative noncoding expressed transcripts are also developmentally regulated.
|Probe type
|Total probes
|Significant probes (FDR = 0.05)
|Overlap: ANOVA and PEAB
|5508 (81%)
|24,062 (89%)
By examining gene expression levels along chromosomes, we discovered that genes that have remained linked during evolution are correlated with one another in expression levels. We considered the genes inside 827 syntenic blocks identified between D. melanogaster and D. pseudoobscura (25), whose expression varies substantially during development (Fig. 2A). We computed the average pairwise expression correlation between genes within each syntenic block and compared with the correlations among nonsyntenic genes. Average correlations among genes within 729 (88%) of these syntenic blocks showed a positive value (Fig. 2B), and genes within 369 syntenic blocks (45%) across all chromosomes showed positive correlations that were significant (P < 0.05) (Fig. 2C). Larger blocks show higher significance of correlated gene expression levels than smaller ones (Fig. 2D). Expression correlations for 104 out of 108 syntenic blocks greater than 300 kbp in length were statistically significant, and all expression level correlations among genes in blocks greater than 650 kbp were significant (fig. S5). These results indicate that chromatin domains or long-range enhancers act across wide genomic regions and that genomic rearrangements in these regions have been constrained during the evolutionary lineages leading to these two species, thereby maintaining these blocks of synteny. Natural selection may therefore act to maintain genes within neighborhoods where expression is coordinately regulated.
Exon expression and splicing. Within each gene, expression levels of exon pairs were typically highly correlated, and the distribution of these correlations was significantly shifted relative to that of exon pairs from different genes (Fig. 3A). A pattern separation algorithm was used to determine the patterns of expression for exons within each gene during development (17). We found three major trends (Fig. 3, B to D): 53% of genes showed uniform or highly correlated (r > 0.8) expression (Fig. 3B), 46% of genes showed multiple patterns of exon expression suggesting alternative promoter usage or splicing (Fig. 3C), and 1% of genes showed multiple patterns with at least one exon pair showing strong anticorrelation during the life cycle (Fig. 3D). These strong anticorrelations suggest exclusivity in the use of one exon or another for this small subset of genes. Together, this initial analysis indicated that a vast amount of gene expression variation is missed in previous microarray studies that have used cDNAs or that assay only a subset of exons from each gene.
However, although these initial analyses allowed identification of exons that are differentially expressed during development, they did not reveal precisely which exons are spliced to one another during posttranscriptional RNA processing. To estimate the extent of splicing in the Drosophila genome during development, we used SJPs to directly assay spliced exons and differential use of splice isoforms. We focused on a subset of 3955 predicted genes that included genes with as few as 2 and as many as 54 exons. For these genes, we designed SJPs for all theoretically possible splicing combinations. Because SJPs could potentially hybridize to exons that were not spliced directly together, we used a specialized set of wrong junction probes (WJPs, splice junctions formed from exon segments but that do not match any possible transcript) as negative controls (fig. S6). These WJPs show higher hybridization signal than the NCPs we used as a reference distribution to detect absolute expression because of partial hybridization. We find that 28% (8732) of the 30,787 SJPs are expressed at a level above the background level defined by the WJPs.
By examining the ratio of significantly expressed, sequentially spliced exons and significantly expressed exons that are noncontiguous, we were able to determine the proportion of exons and genes that are alternatively spliced during development. The ratio of noncontiguous to contiguous splicing (NC/C ratio) for exons' use of downstream exons holds near a constant 0.40 (Fig. 4A), indicating an average of 2.5 contiguous splice events for every exon skipping (i.e., alternative) splice form. Also, these SJP data show that 53% (1374 of 2606) of expressed Drosophila genes from multiexon genes exhibit exon skipping (18).
To determine the extent of splicing that is captured by large-scale EST sequencing, we used BLASTn to align sequences from the junctions' probes to known EST and cDNA databases (Fig. 4B). We identified 3292 splice junctions in the databases that matched our positive hybridization results, whereas 3464 junction probes did not detect hybridization ("EST only" sequences) but did match the expression databases, providing a very high false negative rate of ∼46%. Nevertheless, 5440 new splice variants were identified (Fig. 4B). Most of these (4564) were alternative splice junctions, indicating that EST sequencing missed the vast majority of alternatively spliced transcripts. Taken together, the exon-specific expression patterns and the splice junction expression patterns significantly extend the functional annotation of the predicted genes in the Drosophila genome.
Intergenic and intronic expression. Lastly, we further examined the patterns of RNA expression from nonexonic sequences. There are several reasons to expect that a significant fraction of the annotated noncoding genome is expressed. First, previous studies performed in Drosophila using reverse Northern methodologies on chromosomal walks have identified multiple noncoding RNA transcripts at certain loci, such as in the bithorax complex (26–28). Second, the algorithms and experimental methods used for gene prediction and annotation may have not exhaustively identified the entire gene complement or all of the correct gene structures. Third, transcriptional analysis of a fraction of the human genome (13) and the Arabidopsis genome (12) has shown that about 50% of the predicted noncoding genome is expressed. Thus, we might expect this to be true for other organisms as well, although it is difficult to predict to what extent.
We examined the 41% of noncoding regions of the genome for which we detected transcriptional activity above background. Some of the NEP probes' expression may have been due to previously unannotated genes or to additional exons of already annotated genes from the current Drosophila genome annotation (29, 30). To explore this possibility, we examined whether the expressed NEP probes corroborated computational exon predictions by Genscan (31). Exon predictions not in Flybase annotation (version 3.1) were divided into two groups: those bordering annotated genes and "unique" exons that did not. The latter set includes potential previously unknown genes. Genscan "unique" exons together encompassed 2045 NEP probes, 1221 (60%) of which were expressed. This represents a significant enrichment for expressed probes (compared with 41% of all NEP probes), strongly suggesting that many of these predictions represent previously unknown genes.
The overlapping NEP probes give supporting expression data for 1155 of Genscan's predicted exons. Of these, 369 represent additional exons bordering existing genes, whereas the remaining 786 exons belong to 716 putative novel genes (18). There were also several instances where Genscan predicted a longer upstream or downstream exon boundary relative to the current annotation. NEP probes overlapping these regions of disagreement confirmed expression of 64% (38/59) of 5′ predictions and 81% (30/37) of 3′ ones. Lastly, comparing to a recent study that identified several hundred expressed exons predicted with the Fgenesh algorithm (32, 33), we found a considerable overlap of expressed NEPs within these predicted exons (61% or 477/777).
We next considered intronic expression and found that 43% of introns (6717/15,770) were expressed. There is also a relationship between the activity of an EP and its nearest intergenic NEP (and vice versa). We examined the correlations between these two classes of probes by means of a G test and a Dunn-Sidak correction for multiple testing. The nearest NEP was located on the same or opposite DNA strand in both the 5′ and 3′ direction, and for each of the 15 stage comparisons the differential expression levels of the probe pair were recorded. The stage or sex bias (e.g., transcript enrichment in males versus females) of NEPs at the 3′ end of exons is highly correlated with the stage or sex bias of the exon; similarly, the bias of exons at the 5′ end of NEPs is highly correlated with the bias of the NEP (fig. S7). These results indicate that either these expressed sequences are contained within genes whose end points have been misannotated or that they encode noncoding RNAs that are expressed in concert with nearby genes because of local chromatin or cis-regulatory effects. At least some of these transcripts are likely due to the second hypothesis, because extensive EST sequencing has failed to reveal the transcribed sequences within sequenced exons and because Northern blot analysis (fig. S8) shows that the noncoding sequences we have identified are often contained within several major and many minor polyA-RNA products that are inconsistent with expected protein-coding gene sizes. However, our analysis of the Genscan predictions suggests that many of the expressed sequences that correlate in expression with nearby annotated exons do in fact simply correspond to unannotated exons.
The extent to which these putative noncoding RNAs are functionally relevant awaits strategies for systematic characterization, but this genome-wide scan indicates that they are both abundant and developmentally regulated. The function of such extensively regulated noncoding gene expression during development is unknown. To determine whether these expressed sequences are functionally constrained at the sequence level, we used an alignment of the genomic sequences of D. melanogaster and D. pseudoobscura (25). Sequences corresponding to expressed NEPs were indeed more likely to be conserved than those corresponding to nonexpressed NEPs: The fraction of aligned base pairs was 68.7% for expressed probes and 59.0% for nonexpressed probes (Fig. 5) (P = 10–192, t test), whereas 63.7% (expressed) and 60.9% (nonexpressed) of the base pairs in fully aligned probes were conserved between these species (34) (P = 10–44, t test). This small but highly significant difference in conservation between expressed and nonexpressed NEPs indicates that there are evolutionary constraints on the expressed noncoding portion of the genome. The functional relevance of these sequences could potentially be further tested through methods such as large-scale RNAi screening (22) and systematic mutational analysis or through comparative expression analysis with additional species of Drosophila.
Summary. Ideally, to create a finished and fully annotated expression map of the genome, each stage and tissue would be assayed by multiple methods. Confidence measures of expression levels can have their basis in negative controls and cross-checking between data sets, such as we have presented here. It is clear that our past understanding of genome-wide RNA transcription has been very limited, because a large proportion of exons show dynamic patterns of differential splicing and noncoding activity is ubiquitous. Taken together, our results also indicate that there are thousands of uncharacterized and unannotated transcripts expressed in a developmentally coordinated manner. Systematic genetic approaches will likely be required to determine the functions of the large class of newly identified noncoding expressed sequences, which are slightly more conserved than other noncoding sequence. Additionally, the existence of evolutionarily conserved chromosomal domains of correlated gene expression indicates that these domains are also functionally important. However, the mechanisms responsible for these expression domains remain to be elucidated. This draft expression map of the Drosophila genome shows that there is considerably more complexity in gene and transcript regulation than was previously known, and it represents an initial step in identifying all the functional elements that ultimately control the developmental program of this organism.
Supporting Online Material
Materials and Methods
SOM Text
fig. S1


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This is a variety dish. First onion is fried into a brownish color and added into the chicken marination. Then we will be get a smoky taste for this dish.
- 1 Kg cleaned chicken pieces
- 3 tbsp curd
- 1 tbsp gingergarlic paste
- Salt
- 2 medium onion sliced
Curd, gingergarlic paste and salt saute into the chicken. Then fry the onion into the brown color and crush it into chicken and mix well. Marinate it for one hour.
- 2 onion sliced
- 4 cloves
- 2 cinnamon sticks
- 2 cardamom
- 1 starnise
- 1/2 tsp fennel
- 6 dried red chili + pinch turmeic powder
- 1 tbsp corriander seed
- 3 tbsp veg.oil
- Corriander leaves
- Heat the dried ingredients then grind 1 to 9 ingredients into fine paste.
- Heat the oil and saute the grinded mix well. When the aroma comes from the paste add 1 3/4 cup water in it.
- When the water boils add the marinated chicken in it. Add salt also.
- Allow to cook chicken in a high flame 2 to 3 minutes. Then reduce the flame and cook it for 10 minutes.
- After that add chopped corriander leaves.
- Smoky chicken is ready to serve. Enjoy with chappathi..


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17895 Sweetwater Springs Road
Guerneville, CA 95446 / B0700, SONOMA COUNTY
Charismatic 1905 Victorian farmhouse well-maintained and restored to capture the essence of old world charm. This 2,400 sqft beauty showcases 3BR/2BA with fine craftsmanship and quality oak flooring and cabinetry throughout. Blissful lounging can be attained in the sunroom, on the outside partial wraparound deck or remodeled basement. The romantic 3.5 acre grounds boasts a regal in ground swimming pool and spa exposed to an abundance of sun, surrounded by redwoods & seasonal creek. Not to mention the newer detached garage, gated dog run or animal pasture, flower beds and mature fruit trees. Affording you the ultimate country experience yet having the benefit of being near all the conveniences that Guerneville has to offer.
Contact Agent
Christen Hamilton
This listing courtesy of Christen Hamilton (Lic. 01437477), Vanguard Properties (Lic.01486075).
BAREIS MLS Information herein believed reliable but not guaranteed.
Copyright 2020, Bay Area Real Estate Information Services, Inc. All Rights Reserved.


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Whales In Your Neighborhood
June 3, 2017
12:00 Noon - 2:00 p.m.
Admission Information:
Orca Network, Whale Scout and The Whale Trail are teaming up to provide education about Whales In Your Neighborhood. Meet up with an expert naturalist at a local park to search for and learn more about land-based whale watching and the whales who live in the Salish Sea, in particular our endangered Southern Resident orcas.
This event is part of Orca Awareness Month.
Locations include:
Westside Preserve, Friday Harbor – at the middle pullout
Lime Kiln Point State Park, Friday Harbor – at the observation wall


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Friday Nights with DJ O for TEMPO
Join us Friday nights for a great time out with friends and family.Party starts at 8PM!$12.00 admission$4.50 skate rentals (optional)
Event Celebrations at Rollerplex
Thinking of an event? Join us at Rollerplex, we've got what you're looking for!
Family Day Skate Event
Returning for our 2nd Annual Family Day Skate Event, DJ WIZ + friends will be throwing it down in the DJ booth! Get your skates and make your way down to Rollerplex Entertainment Centre!Monday, February 17, 20202PM-8PM$6.00 Admission$4.50 Skate rentals*No combined coupons and or discounts available*2 HOUR SKATE LIMIT WILL BE ENFORCED.
Hallelujah Skate – Long Weekend Special
Sunday, February 16, 2020Join us with guest DJ PROPHET playing all your favourites in Gospel themed music.This is an all ages-fun for everyone event. Invite a friend and make the most out of this epic night!8PM-12AM$10.00 Admission$4.50 Skate rentals*group rates apply (10+ persons)
Couples Skate – February 14 with DJ O
Friday, February 14, 2020 Couples Skate with DJ O – new DJ on rotation at Rollerplex!Moving from Late Night Skate – TEMPO Friday Nights8:00PM-12AM$12.00 admission$4.50 skate rentals
Family Day Skate Party
Co-hosted with DJ WIZ and friends. This family day is guaranteed to be fun event for everyone! Monday, February 17, 20202PM-8PM$6.00 admission$4.50 skate rentals
Back to the 90s Skate Event
Rollerplex is hosting our first 90's themed skate. Dress in your best 90s gear, and showcase your best dance moves on the rink! See you then! Friday, January 318PM-12AM$12.00 admission


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Energy Efficiency Finance
Option 1Available Mon-Fri 8am-9pm, Sat 9am-6pm
Outlook Series
Outlook is a series of AIB reports that explores key issues affecting particular sectors within the Irish economy.
Our Energy Efficiency Outlook report looks a range of funding solutions and supports provided through traditional bank finance in addition to the tax incentives and grant programmes administered by the SEAI.
To access this and other Outlook reports, click here.
Investing in energy efficient solutions
Investing in an energy efficiency solution today can reduce your energy bills and create future savings for your business. In some cases, the cost of financing your project is less than the savings you will generate.
We have a range of options available to help finance your energy efficiency project. So why not keep cash in your business and finance your energy efficiency project with us?
In AIB we are actively supporting our customers to prepare for Brexit.
That's why we are partnering with the Strategic Banking Corporation of Ireland (SBCI) in the new €300m SBCI Brexit Loan Scheme.
SBCI Brexit Support Loans are available to viable businesses who are Brexit impacted and meet the Scheme criteria which is detailed on the SBCI website.
Finance and repayment options
We provide flexible repayment options – for big or small projects – which can be structured to take into account seasonal changes in your business cashflow. Flexible repayment arrangements may impact on the repayment amount and/or term of the loan.
And, when assessing your credit application, we'll take account of the additional repayment capacity resulting from the reduction in your energy bills.
Our Finance options include:
Asset Finance (Leasing and Hire Purchase)
Our Asset Finance products offer practical and tax efficient ways to finance new or used business assets with flexible and competitive repayment terms. Repayment options can be structured to take into account seasonal changes in the business cashflow.
Energy savings for all!
If your business is …
- facing high energy bills or using lots of process heat
- thinking about investing in new equipment and machinery
- using significant lighting, refrigeration, heating and/or air conditioning
… you should consider energy efficient solutions to achieve a sustainable reduction in energy bills.
Gain control of your energy bills.
Keep track of your energy costs and review energy-saving solutions that can reduce your electricity, oil or gas bills.
We have finance options available to your business. Talk to us.
Replacing old equipment.
When replacing old items such as heating and refrigeration equipment, consider the long-term savings you can make by opting for the top energy-rated solution.
An Accelerated Capital Allowance (ACA)* can help.
*An ACA is a tax incentive that allows companies to write off 100% of the purchase value of qualifying energy efficient equipment against their profit in the year of purchase.
We have finance options available to your business. Talk to us.
Talk to us
We are interested in hearing from you. To find out more on how we can assist you contact:
Elaine Dunne
Email: email@example.com
Tel: 01 7720968
Alan Makim
Email: firstname.lastname@example.com
Tel: 01 7724198
Note: Lending criteria, terms and conditions apply. Credit facilities are subject to repayment capacity and financial status and are not available to persons under 18 years of age. Security may be required. Flexible repayment arrangements may increase the cost of credit.
Business Reports / Articles / Tip Sheets
- Outlook: Energy Efficiency for Business
- Energy Saving Tips … Office Space
- Energy Saving Tips … Hotels
- Energy Saving Tips … Retail
- Energy Saving Tips … Manufacturing
- 10 Tips to Successfully Select a Biomass Boiler
- 5 Tips for Selecting LED Luminaires
Case Studies
- Energy Management Case Study: Centra
- Energy Management Case Study: Riverside Park Hotel and Leisure Club
Sustainable Energy Authority of Ireland (SEAI) Resources


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1st Choice For Quality Garden Sheds & Buildings
Craig Antill --- 1st Choice Leisure Buildings --- To keep up to date please visit our Facebook page
Take the opportunity to browse our range of buildings online & then come down to see us, a family business started in 1979.
Would you like your own space in the garden for your hobby? Then take a look at our TIMBER WORKSHOPS – Take a look at our delightful TRENTAN LOG CABINS with sizes and styles to suit all. – Enjoy your garden with your family in a new SUMMER HOUSE. They will love you for it. – Does your car require a home? We have all the LIDGET CONCRETE GARAGES range to suit all ranges of vehicles. – Would you like to work at home? Then why not consider a GARDEN OFFICE made to suit your needs. – With two large display sites you can visit us anytime or easily shop online.
How To Choose Your New Quality Garden Building For Your Home
Farnham Show Site
1st Choice Leisure Buildings
Squires Garden Centre,
Bagshot Lea Road,
Badshot Lea,
Phone 01252 311085
Woking Show Site
1st Choice Leisure Buildings
Sutton Green Garden Centre,
Whitmoor Ln,
Sutton Green,
Phone 01483 237550
STOP And Think BEFORE Buying Your New Garden Shed. What you should ask and check BEFORE buying
- Are the buildings you want to buy on display?
- How good is the wood cladding?
- Are the floor and roof SOLID timber – is there any chipboard?
- Can you choose your size and style of shed?
- Are the sheds delivered and installed FREE and delivered FREE?
- How thick is the framework?
- How tall are the buildings, are you a midget?
- What conditions are attached to the guarantee?
- Is the shed made in the UK and is it stockpiled?
Viewing and Display Sites
Timber Cladding And What To Look For
Your New Shed Roof Is Crucial To Its Life
Your New Shed Floor Needs To Be Strong
Ensure The Roof Style Will Suit Your Needs
How Much Does It Cost To Install a Garden Shed? – What You Need To Know
Read About Delivery and Assembly Options
Important To Know About Windows And Glass
If You Are Not From Lilliput Then Be Wary About Your New Building
And Best of All – THOSE Wonderful Extra Long Guarantees Being Offered
How Much Are Garden Sheds?
This all depends on the type of garden shed that you decide to buy and a whole host of other options. There are so many options to choose from and these will depend on the purpose of the building. Take a look through the product tabs at the top of the garden sheds page and go to the appropriate section. There is a huge choice from plastic to concrete and wood.
Many have a range of build and installation options (free on small and large wooden and concrete sheds). Some have insulation options as well to keep you warm in winter and coll in summer. It will also depend on where you want to site your shed. Whether this is in the back garden on at the side of your house. Each page has full help and advice on design and build options.
Personal Quotations?
We are very happy to supply you with a free no-obligation quotation for your heavy duty garden shed.
Call us on 0333 800 8880, use our contact form or visit one of our display sites.
Garden Sheds Over The Years
What Is A Shed?
What Materials are Garden Sheds Supplied In?
These buildings come in a variety of materials such as wood, timber, metal, steel, plastic or concrete. Often the choice of material used for these tends to be personal choice but different claddings have varying pros and cons. Timber sheds tend to be most popular due to the natural feel it the material gives. However, whilst they are strong, one of the downsides is they need to be treated to maintain their long life. Tanalised or pressure-treated ones won't require as much maintenance. They normally last for around 15 years, so are very popular. Some sheds are made from galvanised steel with a long guarantee against rust, so no treating of these sheds is required. Beware of metal buildings which are 'electro-plated' rather than 'hot dipped galvanised'. This treatment won't last as long. Sheds made from more durable materials won't rust so are a viable proposition for the owner who does not want any maintenance issues. However, these type of units can look a little bland and industrial but do serve their purpose in the garden very well.
Concrete is the 'bruiser' of the shed world. Being made from solid concrete they don't need any maintenance to speak off. They can brush aside everything that the weather throws at them. One of the downsides of these is the limitation in sizes available and. Also, to some people, they can feel rather utilitarian.
1st Choice for Corner Sheds and Summer Buildings
1st Choice for Wooden Buildings©
How To Insulate a Garden Shed Floor, Walls and Roof
- 1/ Measure the size of your building. How high are the walls, how large is the roof area, measure the floor if you want to insulate it
- 2/ Decide on the type of insulation you would like to use. i.e. fibreglass type, insulating a shed with polystyrene, or foam insulation sheets
- 3/ Order the selected insulation from your builder's merchant or someone like B&Q
- 4/ How to insulate the garden building walls – Cut the insulation to size and fit this in between the walls and tack into place.
- 5/ Cover the wall with plywood, hardboard or T&G timber boards.
- 6/ How to insulate a building roof – Cut the insulation to size for the roof, we recommend using polystyrene as this can be cut slightly oversize so it can be wedged into place
- 7/ Cover the roof with plywood or hardboard. We recommend screwing this into place so it can be removed if need be.
- 8/ Finally cover the joins in the wall and roof panels with cover strips. This ensures your insulating of your shed, summerhouse or hut has a tidy cosmetic finish
- 9/ How to insulate the floor – You will need to fit insulation in between the floor joist and tack into place before assembling it.
- 10 Alternately, buy an shed with insulation already in place. This is the easiest option but a little more costly. See our full range of insulated garden sheds on this page.
Should I Consider Buying An Insulated Shed?
The garden shed is used traditionally as a storage place and sometimes as a workshop which limits its use, especially during the cold winter months. There is another option which means you are able to use it all year round. By investing a little more and upgrading to an insulated building you will be able to use these wonderful sheds all year round. Your children could use it as a den and maybe play computer games in there or chill out with their friends.At at the same time, they will be out from under your feet! Mum or Dad could use it as a 'man cave' or 'she den' for their own hobbies, pastimes and have some 'me' time. An insulated shed could be used as a home office for business. If so the cost would be tax deductible which is a 'win-win' for you.
Throughout the summer months, it's no problem using your building in your garden for the normal day to day things, whether it's a little pottering about or enjoying being away from the house. But in the cold winter weather, it becomes a place not to be used. Which is a shame? How much better would it be if you could use it all year round with no limitations on what you could do down there.
Well, this is possible by deciding to go for an insulated one and you will then not mind going down there all year round. You would then be able to enjoy your hobby down there, possibly allow your children to use, but with reservations on that use, or maybe just enjoy a little time away from the hubbub of the daily grind. If you had a little 'home cottage' type business and used in connection with that activity then you could claim the cost against your profits so that would make it even more feasible.
Can I Insulate My Shed After It Has Been Delivered?
Are There Any Other Options Available?
Will my Insulated Shed Be Warm In The Winter?
Provided your new shed is insulated and lined either by you or us then it will be nearly as warm as inside your house. However, like your house, during the colder spells, you will require some heating to ensure a comfortable environment.We would recommend an electric radiator as the best option. Stay well away from any form of Calor type gas heaters as they produce a lot of damp as well as being a fire risk. The bonus is that the insulation will help to keep your shed cooler in summer.
This is something I didn't know in my early days of garden shed manufacturing (I started back in 1979 making sheds). I discovered this when going from an uninsulated building into an insulated one. The difference was amazing! The insulation option is well worth considering
How Much Will Insulating A Garden Shed Cost?
Can An Insulated Shed Help Sell My House?
The attraction of an insulated garden shed is very appealing as this can give prospective purchasers a very good reason to choose your house. This will give them an opportunity to carry on using it as an office, or maybe they can use it as a recreation or craft room. Possible other uses are as a gloried den for the children which they would love. You can always look on the internet and ask what is the cheapest way to insulate a garden building or about insulation DIY options?The choice of garden buildings is staggering with many different types available and you can see a good range on our web site. The most important consideration is to ensure it is lined and insulated (I always think this should be insulated and lined as the insulation has to go in before the lining. But it normally is described as lined and insulated, (why I don't know). The reason for the insulation option is to help to keep the building warmer in winter and more importantly, and not always appreciated, is to keep it cooler in the summer.
You need to ensure that the internal height is sufficient as many of the cheap (made down to a price) sheds and garden buildings are quite low. This is important as a low ceiling height will make it a little claustrophobic. Ensure that the building is made from T&G (tongue and grooved) timber and also, for long life, from redwood deal and not the cheaper white wood.
Where ever possible order double glazed doors and windows as this will help to keep the warmth in during the winter and the heat out during the summer making the building pleasant all year round. There are a number of options for the roof covering from heavy duty felt, long lasting Onduline roofing, felt tiles, cedar shingles or even a slatted roof. All will keep your new building dry from rain and snow so the choice is cosmetic.
The Managing Director of property developers Puddlestone said that there has been a significant rise if the demand for homes with insulated buildings in the gardens. This has made those homes more attractive and easier to sell. This is put down partly to the increase in people wanting to work from home due to the financial crisis. Having said that it does not always mean that the sale value will reflect the cost of the home office just that it makes the home easier to sell.
If you are thinking of investing in an insulated garden shed for the garden it is good to know that it will help in the future to sell your home. So you have had the benefit of whilst you are living there and you also have the confidence that when you decide to move on it should be easier to do so.
Can I Receive A Personal Quote?
Our Corner Garden Sheds Add Value to Your Home.
Read The Following If You Have An Unused Corner In Your Garden For A Shed
Why You Should Choose a Corner Shed for Your Home
Before Buying a Corner Garden Building Always Do Your Home Work
When Choosing Always Beware of Cheap Timber Being Used
Be Very Wary of Cheap Chipboard, OSB Board and Other Cheap Boards
Our Large Range of Sheds Will Be Built For You. Taking the Worry Out It For You
Where Ever Possible Try To View Your New Shed Beforehand
Garden Sheds For Sale
All available in more or less in any size to suit you. All wooden sheds include free assembly saving you loads of money and making life just a little easier. They can be made bespoke to suit your needs in a range of styles, sizes and finishes.
When looking for garden sheds it appears very straight forward. Just click around the internet, find the size you want and then look for the best price. Easy YES? Well not really as there is such a huge array of varying quality sheds out there.
From the cheap and nasty right up to ones which would feel at home at Hampton Court Palace.
You need to do your homework to ensure you buy the right one for YOUR needs. Take a read of the following or look on our What You Need To Know web page or any of our Top Buying Tips pages.
1st Choice Leisure Buildings – ONLY Sell Garden Sheds Made to Very High Standards
By choosing to buy your timber shed from an established business you can be sure that they have a great deal of expertise in the industry. Also, they have become established by selling the right products at the right price.
Most importantly, they have been listening to their customers. By choosing a business which actually has a show site you can be sure there is somewhere to contact them if you need to. With online-only businesses, they can be here today and gone tomorrow.
With a business with an actual physical address, you will also find you can talk to someone if you choose. Just by typing in 'sheds near me' or 'garden shed suppliers' will generally find a local business.
1st Choice Leisure Buildings, a Family Business which was established in 1979. We also have a physical office and Show sites.
Toughened Glass As Standard
Sheds by their very nature are large and heavy so building them can be a challenge. This may be fine for some people but a company offering a delivery and installation service will always be a better option. Not only does it save you a lot of hassle, but you can also be sure it has been installed correctly.
Besides the assembly service for our sheds, we can also arrange for a base to be built. This can either be made from a slab of solid concrete or a tanalised timber sub-frame base. It's even possible to arrange for your building to be painted in colours to suit you. Although the buildings do come with a protective treatment.
1st Choice offer a delivery & assembly service (mostly free of charge) to save you time & effort. Not just dumped in your garden.
Bespoke Garden Sheds
The internet is a wonderful thing but even with the flashiest of pages or glossy brochures, you can never get a REAL feel for what you are buying. For many items, this is not a problem but with your new garden shed it's important to know that what you getting will meet your expectations. By actually viewing the proposed structure. you can be sure of the quality and how well it is constructed. You can see how tall it is so you won't bang your head. Also how the doors and windows are made.
This will help you to distinguish from the cheap and nasty built to a price. Our well-constructed buildings are designed to last a long time. By viewing beforehand you can save yourself from potential heartache and stress. You'll be glad you did!
The cheapest price is not always the best value. Click Here For A Walk Through Of Our Display Site
Visit One of the 1st Choice Leisure Buildings Showrooms – and see our displays near Guildford and Farnham in Surrey.
There are certain advantages for standard sizes or designs as these can help keep the costs down. However wouldn't it be good to buy a building to suit you and your garden? By doing this you can utilise your new shed to have the best use and to fit in the space that you have.
Talk to a company which can make a bespoke structure in the size and design you need. The extra marginal cost can be the difference between making do or being 100% satisfied.
All you need to do is to talk to us about what your ideal shed is and we will do our utmost to meet your needs.
1st Choice Leisure Buildings can make virtually every shed we sell in a size and design to suit you.
Onduline Roofs With Air Gaps
Money can always be saved by selecting a cheaper timber such as whitewood. To a certain degree, it's OK for a basic garden shed. However, It's far better to insist on a quality redwood 'deal' timber. This wood grows far more slowly than whitewood so the grain is more tightly compacted, ensuring a longer life. A marginal extra expensive for a better long term prospect. Whitewood, because it grows quickly can be subject to the small knots dropping out.
All timber is responsibly sourced and is FSC 'Forest Stewardship Council' quality approved. This is very important these days where every effort needs to be taken to protect our environment.
The majority of 1st Choice's garden sheds are made from redwood 'deal' timber for a long life.
Traditional Style Garden Sheds
You will find that most of the cheap garden sheds use chipboard, OSB, or Sterling board on the floors and roofs to save money. However, as soon as damp gets in these boards start to swell and disintegrate. That's the end of your shiny new shed!
Some shed companies try to hide this by saying their floors and roofs are made from 'Sheet Materials'. They avoid using the words chipboard or OSB board because they know there could be problems in the future.
1st Choice Leisure Buildings recommends solid timber – we do NOT use chipboard, OSB board or Sterling board!
An Ideal Solution For Your Home
Many of the cheapest shed companies deliver them in pieces where you have to build some of the panels yourself. Ludicrous! If you are buying a cheap shed always triple-check what you are getting! Your new buildings are unlikely to be pre-constructed, leaving you with a load of work to do. It may seem like a great idea to buy a kit. But know what you are letting yourself in for!
Perhaps when you left school you saw your future as a carpenter or a 'chippy'. You may have been looking forward to a life building sheds. That's fine! There's nothing wrong with that. But if not then why don't you let the factory's do the work for you?
Insist on a complete building to be delivered to you.
All our Buildings are delivered in completed sections (and mostly assembled free of charge) to save you time and effort!
Visit this page for more Buying Advice You will be pleased that you did. Also, visit 1st Choice's UK display sites and see for your self. There is a great selection of sheds built on our show site for you to view!
Before spending your money take the time to out visit one of our two display site in Surrey, close to Guildford, Woking, Farnham and Aldershot. Our friendly staff will be delighted to show you our buildings, listen to you and give you free unpressured advice to enable you to make an informed choice.
Our Range Of Summer Houses
If you are interested in a new summerhouse, sun room or summer building for your home – whether it's for sitting out in the garden on a sunny day, or for using the summer house for a recreation area or hobby room or somewhere to store your leisure equipment or gardening supplies – then you find will find a large range on offer on our 1st Choice Leisure Buildings web site.
Our wide range of summer houses, sun rooms, home offices and garden studios are available to be delivered and assembled at your home. All these buildings are ideally suited from small back gardens right up to very large gardens and can be used for domestic, recreational or professional use. To suit the needs of you all our buildings, which include log cabins, sun rooms, summerhouses and wood built home offices can be bespoke, both in size and design, so it meets YOUR requirements. Why settle for anything less? We always strive to ensure we are your 1st Choice for your garden buildings.
Our Range Of Garden Workshops
When you are looking for a new garden workshop, timber or sectional building you need to do your research. Take a look on the web and see what is available – you will find a large range of differences in price. And that is because of the quality (or not) of the building. A workshop for your garden can be made very cheaply and down to a price but won't last. Or these garden workshops can be made properly with the right grade of timber and right construction methods.
All our Platinum and Diamond workshops are made in the right way with the right materials so they will be buildings you will be proud of. Add in the free assembly service and you can see what great value our buildings are. Designed to last and to be useful in your garden
Our Range Of Garden Offices
In our modern lifestyle things are changing and more people are looking for a better work – life balance and this is why working from home is becoming more popular. To address this need our range of garden offices for sale has been increased with new and more up to date features to ensure the best environment for you. Our Royal garden offices are hard to be beat anyway and include all the very best features to make working at home a pleasure.
The best piece of advice when considering a garden office is to ensure you do your research an above all ensure that you view your prospective building before buying. This way you will ensure it will meet your needs and expectations. Take a trip out to one of our Surrey showrooms and see for your self. Being a family business we will be happy to offer free advice to help you choose.
Our Range Of Trentan Log Cabins
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Ordnance Park was a neighborhood built to benefit the many families (mostly women) who worked at the Naval Ordnance Plant in South Charleston, WV, or B. F. Goodrich Rubber Plant in Institute, WV during WWII. MacCorkle Avenue was later constructed to accommodate increasing traffic.
The homes were rentals until after WWII, when they were offered to returning veterans. There were 225 duplex houses with two different floor plans of two or three bedrooms. The community had a school, a church and a movie theater. Four playgrounds are still being used today by current residents.
The street names throughout Ordnance Park are named after President Franklin D. Roosevelt and prominent naval officers and contractors that worked on the site.


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In the Osten lab, researchers focus on the analysis of brain regions and how brain cells connect and communicate with each other to drive behaviors. This communication between neurons may be disrupted in disorders like autism and schizophrenia. The lab uses the latest technologies to image entire mouse brains and to map brain-wide neuronal activation evoked by various behaviors, including social behavior. Dr. Kim and his CSHL colleagues try to identify the specific cell types in the brain regions involved, and test the role of different cell types and brain regions in mediating specific aspects of the behaviors. The goal is to identify targets for therapeutic development.
Congratulations to Yongsoo, recent winner of a coveted NARSAD Young Investigator Award, which he will use to pursue research on the hormone oxytocin in a mouse model of autism. Because oxytocin has been associated with certain behaviors including social recognition, bonding, anxiety and maternal behaviors, it is sometimes referred to as the "bonding hormone." Yongsoo is looking at which brain regions respond to doses of intranasal oxytocin, and how administration of this hormone alters brain activity in response to social stimulation.
About SCIENCE SHORTS: Each short talk of approximately 5 minutes explores a topic or area of research at CSHL. They were designed for non-scientific audience and given by a CSHL Ph.D. student or postdoc. So far we have covered the question "Why haven't we cured cancer yet?"; the beautiful imagery generated by the important scientific technique of florescence microscopy; and, how protein structures are determined using "molecular photography."


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Hi! We're Bring You Online.
Hi! We're Bring You Online.
When you sign up with Bring You Online, we ask you for some details about your business, such as your address, contact information, services, prices and working hours. The more information you provide, the more personalized your site becomes.
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Great question! Here is a list of some of them:
Never! We believe we will work for you, and that you should be able to tell us if your business goals realign. We realize things happen and are here to help you.
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We are a small family-based, veteran-owned agency out of Scottsdale, Arizona with hundreds of clients that rely on us to help grow their business online, increase conversions, and help with their online presence.


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For years, the profession has complained that the RCVS does not listen to its members, that council members were not representative and yet voting numbers in the RCVS elections have been low for years, although now improving. BVA and its specialist divisions, including BEVA, have been the route of communication, holding them to account. The First Rate Regulator initiative calls for it, amongst other things, to enhance engagement and communication with the profession and for it to be seen as a modern and relevant regulator.
Recently the RCVS announced its plans for the middle tier; advanced practitioners. This is a welcome innovation, recognising a range of post graduate qualifications including certificate exams. There are factors that many are still unhappy about; the £110 price tag for the title and the requirement for old style certificate holders to complete A module CPD. This is particularly galling to some since there is still a perception that old style certificates were more rigorous. I don't believe this is fair, and the inclusion of some of these 'soft skills' does not decrease the rigorous clinical components in the C modules. Candidates work just as hard, if not harder for these qualifications and we should avoid devaluing their efforts.
A second aspect of the proposal is to remove most post-nominals from the register. This has caused considerable consternation in the profession. This proposal would mean that only a registrable degree would be listed. All certificates, masters (including the MA awarded at Cambridge), doctorates and diplomas would be replaced by the listing as advanced or specialist practitioner. The idea is to simplify public understanding and mirrors what is done by the General Medical Council. An online petition generated over 1200 signatures, while only around 300 had responded to the initial consultation in 2011. Why did so few respond to the working party, and so many respond to a petition? Have we become more engaged with veterinary politics, or were the proposals inaccessible to the members? The obvious response is that they were clearly accessible to all, details were in the RCVS news and widely publicised online. Everyone could read them, but it is evident that very few did. Accessibility is not just about availability.
I suspect, as so often is the case, that size is everything. We are all busy, few will prioritise a 33 page document that has no clear executive summary, especially with a title of veterinary specialisation; at the time of the report there were around 2500 certificates passed, yet only around 300 RCVS specialists. Its title suggested it was not relevant to the majority. Deep within this document was the question Do you agree that there is a need to simplify the range of qualification titles and postnominal letters that are shown against veterinary surgeons' names in the RCVS Register? BEVA responded on your behalf and as a result the proposal to only indicate broad areas of work (equine practice) were changed and the advanced practitioner status will now indicate specific areas of work (equine internal medicine, equine surgery, equine reproduction, equine practice). The removal of post nominals from the register remained.
So what made so many respond to a petition? Why are the numbers of signatories more than half the number of certificate holders (there are less certificate holders than there are certificates passed as some have multiples)? It is clearly all about methods of communication; news of this petition spread by social media, by online forums and via email. Short messages, easily digestible, that asked for people to respond to a single question. All that was required was signing a petition, although many have also left comments. Size was everything!
The world has changed, the internet bombards us with information. It has made us good at digesting small bits of information, and shunning long blocks of text. Twitter and text messages limit us to 140 characters, we have grown use to this. Many of us are disinterested in emails that are longer than one screen, irrespective of the device! Short IS sweet. The RCVS need to wake up to this new reality and be more concise in its communications. If they want engagement, then they need to develop ways to encourage this in a way that is relevant to the 21st century. If they don't they are going to be faced with a great many more 11th hour petitions and resentful members.
Many of us were surprised by the response of the college, to review this proposal, to respond to its members! The president even took to Twitter to share the news and engage in debate. Is this a new era in listening from council? Are they living up to the aspiration of 'first rate regulator'? Their response did suggest that the profession has 'misunderstood' the proposals, the same word was used in response to the last petition about out of hours provision. I hope that they have not misunderstood the feeling from the profession on this issue. We DO object to paying £110/year to be listed as advanced practitioners, when listing a certificate has cost nothing, but the biggest objection is to these qualifications being removed from the register. In the words of Jesse J 'Its not about the money.' If the outcome of the RCVS review in June is to simply reduce the costs of the list they will demonstrate that nothing has changed in terms of their ability to respond to the membership. If they review the whole proposal they will show that we really are entering a new era of dialogue and engagement.
What was interesting in this online debate was the lack of involvement from the BVA. No comments, no tweets, nothing. While BEVA helped spread details of the petition, BVA clearly showed no interest. They did create an entry in their community pages, but this was the day after the RCVS announced their review. It never made it to the Vet Record. This is an organisation that is meant to represent the profession, who appeared to not notice until the RCVS issued a press release. They need to be careful, if the RCVS are going to engage directly with the membership then the BVAs role as conduit for the profession to lobby the RCVS will be lost. People may start asking what us the point of the BVA.....


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Trade for new and used items, like cheap adidas shoes, in in Toronto. Earn BTZ to buy and sell on Bunz, or to spend on coffee, groceries and more at local businesses. Help us support sustainable trade in Toronto.
Comfy Brown Chair for Cheap!
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REVIEW: Doctor Who – The Lodger
Once again, this was an episode I was sure I was not going to like after watching the preview. I'm happy to say that I couldn't have been more wrong. It's one of the stronger stand-alone episodes of the season and looks to be the last chance to catch your breath before the season finale.
Spoilers next.
The episode is framed with the TARDIS being unable to fully materialize on modern day Earth after essentially spitting The Doctor out and disappearing with Amy still aboard. Separated from the TARDIS and alone on Earth, The Doctor takes up residence in a room for rent with a guy named Craig where people are continually lured up to the second floor by the neighbor and never come out.
Along the way we see as many different people luring victims upstairs as we see victims. Whatever is happening to them is causing time distortions that are blocking the TARDIS from landing. The Doctor can't risk being found out in his investigation, so he can't use any advanced technology and has to do his best to fit in. This includes The Doctor recruiting a cat as a spy, working in a call center, and dazzling a pub league soccer team which is a lot of fun and nicely timed with the World Cup Finals this weekend.
In the end it's revealed that the whole of the second floor is actually someone's attempt to build a TARDIS with a perception filter and the "tenant" is failsafe software trying to repair the ship and find a suitable pilot. It has been burning through humans in its search, but now that it has found The Doctor it selects him to allow it to escape. The Doctor saves the day by using Craig's desire to stay right where he is to convince the ship to shut down and self-destruct.
A stranded spaceship with a dead crew and a perception filter that warps time and has been looking for pilot… Could this be The Doctor's TARDIS? Is this its fate after the explosion at the end of the universe? The Doctor found a fragment of the TARDIS door in the crack and there's a good chance there's a connection here. We also see the crack appear on the apartment wall after The Doctor leaves and Amy finds Rory's engagement ring after asking The Doctor why he can't find her a man. Everything is building to a head and the preview for next week looks absolutely amazing.
More on Doctor Who 2010:
Vincent and The Doctor / The Pandorica Opens
Join the Discussion
Mentioned in:
- REVIEW: Doctor Who – The Pandorica Opens » My Latest Distraction
- REVIEW: Doctor Who – Vincent and the Doctor » My Latest Distraction
- REVIEW: Doctor Who – The Impossible Astronaut » My Latest Distraction
- REVIEW: Doctor Who – Day of the Moon » My Latest Distraction
- The Quotable Doctor Who » MyLatestDistraction
- REVIEW: Doctor Who – The God Complex » MyLatestDistraction
- REVIEW: Doctor Who – Closing Time » MyLatestDistraction


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I'm so excited to tell you about our three Team Orphans Virtual Ironman teams! What a fun way to raise money for adoption and get a lot of people exercising! With three teams and 82 members, we'll be exercising many thousands of miles! Meet the three team leaders today. For the next few months, I'll be introducing you to all of our team members from around the country. We have a wide variety of people (and one dog) – ages from 8 to 70, and athletes of all different kinds- runners, bikers, swimmers, walkers, dog walkers, ice skaters, and roller bladers.
Team 1 is headed by Denny Gillen. Denny is 61 years old and is training for his 3rd Ironman race, Ironman Boulder, on August 3. Denny has already raised over $18,000 for Team Orphans. He is a sales rep for Hewlett Packard and is responsible for HP becoming a business sponsor and for our Team Orphans clothing. (By the way, our Team Orphans clothes- tri shirt, tri shorts, biking jerseys, and running shirts- are also fundraisers. For every purchase, 25% is donated back to our adoption grants.) Denny is the proud father of 3 sons and the grandfather of 11. His beautiful wife, Stella, has lovingly and patiently endured Denny's biking accidents lately. Thanks to all of our prayers, Denny is healthy and training well!
Team 2 is headed by Brian Helsel. Brian is our young ( 24 yrs old) and fast Ironman! Brian just finished his Master's of Science degree in exercise and sports medicine with a focus in exercise physiology from Western Michigan University. Last week he moved from the Grand Rapids area down to South Carolina to work as the Healthy Living Director for the Spartanburg YMCA. Other future plans are to race Ironman Louisville on August 24, fundraise for Team Orphans, and promote endurance sports and a healthier lifestyle to the community.
Team 3 is headed by me-your crazy Team Orphans leader. My next Ironman is November 1 in Panama City, Florida. In addition to directing Team Orphans, I'm also a wife, mother of three teenagers and two spoiled cats, church pianist, cookie baker, and flower gardener. Interesting fact about me? Eight years ago I had never run or ridden a road bike. All I ever wanted to be was a music teacher…..
So we have three Virtual Ironman teams going on now! Denny's Team has 19 members and started in March. They have already traveled 4, 751 miles. Brian's team has 7 members and they just started a few weeks ago. They already have 422 miles of exercise. My team, now at 56 members, starts today.
I'll be blogging every week about three of our team members. Enjoy reading about each other. Thank you for supporting Team Orphans and adoption. ……because every child deserves a forever family……


text: This special volume originates from the International Conference on Innovation and Management held at University of Vaasa in Finland in 2014. Talks with the key note speaker and Editor-in-Chief of the Journal of Cleaner Production led to an idea to develop a special volume about innovative products and services according to the themes of the conference. Thus, the purpose of this special volume is to explore different viewpoints of how innovative products and services may support sustainable societal development. There are five thematic areas with papers that describe new advancements in different industries and organizations. The included papers cover relevant theoretical background and present case studies and practical results. This special volume shows that great progresses are being made in different thematic areas but also that there are so much more waiting to be done for sustainable societal development. This volume indicates that cross-disciplinary approach is truly needed to achieve societal sustainable development. This requires people to change their mindsets and genuinely co-operate towards better future. (C) 2017 Published by Elsevier Ltd.


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India Arrangers ANZ Investment Bank, BA Asia and Crédit Lyonnais are waiting for responses from two banks before closing the $110m equivalent yen denominated loan for Reliance Industries. The borrower is deciding whether to increase the size of the deal or to scale back allocations as ...
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Indian River arts festival moves to September
Those who attended an arts festival in July at the Indian River Marina in past years and loved it, but thought maybe they'd somehow missed it this year — they are in luck — as is anyone looking for a fun family activity.
The Autumn Art Festival, set for Saturday, Sept. 14, at the marina, is a reboot of the seafood and art festival previously held in the third week of July, according to Delaware State Parks spokesperson Jayme Gravell.
Because July is peak boating and beach season, organizers felt moving the event to September might make it more enjoyable for everyone, from the vendors to the festivalgoers, Gravell said. With the start of the "shoulder season," parking should be much easier for the Autumn Art Festival, she said.
The festival, which runs from 10 a.m. to 5 p.m., will feature many of the same artist and vendors, as well as a variety of food trucks, entertainment, children's activities, and a beer- and wine-tasting area for the adults.
In addition to a selection of handmade wares and other retail vendors, the festival will feature entertainment by steel drum musician Jimmy G, as well as an appearance by Lollipop the Clown from 1 to 3:30 p.m. Pumpkin painting activities (painting pumpkin cutouts) for children will help usher in the fall decorating season, and there will be pinatas for the kids to have fun with as well.
Hammerheads Dockside, located at the marina, will host a beer- and wine-tasting venue from 11 a.m. to 4 pm. Food trucks will be parked around the pavilion during the entire festival, with local favorites including Fat Daddy's BBQ from Georgetown.
The artists will be lined up along the bulkhead area of the marina.
There is no charge for admission to the festival, and parking is also free of charge.
By Kerin Magill
Staff Reporter


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INDIANAPOLIS, Ind. – (WTTV) Police say a woman was killed while waiting for help on westbound I-70 near the airport.
The crash occurred around 5:30 a.m. Authorities say a woman was parked in the emergency pull-off lane with an apparent flat tire near the ramp that leads to Ronald Reagan Parkway and the airport.
While waiting inside her SUV for help, a Ford black pickup truck came off WB I-70 and struck her on the driver's side. She was pronounced dead at the scene.
The male driver of the pickup truck was taken to the hospital with non-life threatening injuries.
There are major backups in the area. Avoid it by using US-40 through Plainfield as an alternate route.
We will update this story when more information is made available.
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- Police identify the victim of Friday night fatal crash on I-70
- Heavy rain to blame for I-70 crash
- Several vehicles involved in I-70 Crash


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As a third-generation, family-owned business, we have been in the plumbing industry for nearly 80 years. In that time, we have garnered a wealth of experience in residential and commercial plumbing repairs, installations, and maintenance. Whether the commercial plumbing repair you need is large or small, our extensive knowledge from years of experience enables us to solve your plumbing issues with precision and efficiency. With integrity as a top priority, you can trust us at Bodenheimer Plumbing Services, Inc. to not tack on unnecessary fees, but instead to do your repair at a fair and honest rate.
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Don't allow your plumbing issues to wait so that they can grow in significance and don't allow them to interrupt the flow of your business any longer. Call us at Bodenheimer Plumbing Services, Inc. today for your commercial plumbing repair.


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For Sale $45,000 - Land
This HUGE lot (over 3/4 acre, almost 1/2 manzana!) is located in the mature development of Los Miradores, just north of San Juan del Sur. It offers incredible bay views, and both electrical and water connections. Only five minutes from town, yet the tranquility makes it feel miles away. THIS IS THE BEST BARGAIN CURRENTLY AVAILABLE within Los Miradores.
Total lot area: 3164 m2, 34,000+ sq ft, 4493v2.


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The Pressure is ON!
1 John 2:18 "Little children, it is the last hour; and as you have heard that the Antichrist is coming, even now many antichrists have come, by which we know that it is the last hour. They went out from us, but they were not of us; for if they had been of us, they would have continued with us; but theyRead more


text: Quick view Bourjois Paris Bourjois Face Air Mat Powder 01 Rs3,000.00 Ultra fine and lightweight formula. Even & flawless finish. Ultra-lightweight formula that provides bare skin sensation. Mattifies the skin and blurs imperfections for a flawless finish. Does not cause dry skin feeling. Add to Cart Add to Wish list