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Tag: John Garfield ()
The John Garfield Story- Before Brando, Clift and James Dean, There Came The Arrival Of The First Genuine, American Movie Anti- Hero. Narrated By John's Daughter, Julie Garfield- One Hour
"They Made Me A Criminal", "Body and Soul", "Force Of Evil." These 3 films articulate the range of one of America's finest actors. He inhabited a place in film that few performers dare exhibit. In actual life, he fought for progress. In doing so, he left a legacy and the world at age 39. 1 Hr.
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Summer Food Service Program
The Parma City School District provides free summer lunches to all children 18 and under. The free lunches are offered regardless of what school the child attends or what a child's pay status is when in school. Free summer lunch is offered at Dentzler Elementary School, John Muir Elementary School, Greenbriar Middle School, and Shiloh Middle School.
Click this link below for more information about the Summer Food Service Program:


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Stressed and Fatigued?
By Dr. Donald L. Piccoli on 05/07/14
Stress and fatigue are, by far, the most common health complaints for women over 50. There may be a myriad of other issues on the complaint list, however, stress and fatigue are almost always somewhere in the mix. Stress has been a daily buzz word in our lives ever since Dr. Hans Selye's published his "General Adaptation Syndrome" and coined the term "stress." Stress has become something we think we all should be free of, something to avoid at all costs, the boogey man of modern life, lurking in the shadows at home, on the job, in the car, and affecting everything from relationships to career success, finances, and health. Indeed, it affects all areas of our lives.
"I was a little excited but mostly blorft. "Blorft" is an adjective I just made up that means 'completely overwhelmed but proceeding as if everything is fine and reacting to the stress with the torpor of a possum.' I have been blorft every day for the past seven years."
Tina Fey, Bossypants
The healthy human stress response has always been necessary for survival. Thankfully, our species has always had the ability to respond appropriately to a perceived danger of a life or death situation. Going back to the caveman days, if you were being chased by a buck-toothed giant rodent that had visions of you as its main meal, your primary means of survival may have been your body's ability to initiate the fight-or-flight stress response.
Most of today's stresses come from the physical and emotional traumas of everyday life. Instead of being chased by a wild animal, stress can come from our jobs, businesses, finances, traffic, spouses, parents, and kids. Stress has many negative health consequences, and for women they include chronic fatigue, hypothyroidism, depression, lowered sex drive, sleep loss, weight gain, diabetes, early menopause, panic attacks, heart palpitations, cardiovascular events, and digestive issues.
"Pain is a relatively objective, physical phenomenon; suffering is our psychological resistance to what happens. Events may create physical pain, but they do not in themselves create suffering. Resistance creates suffering. Stress happens when your mind resists what is… The only problem in your life is your mind's resistance to life as it unfolds."
Dan Millman
The brain has different types of receptors for the various female hormones making women much more exposed to the many dangers of the stress response. Women are much more likely to develop auto-immune conditions as they age, also due to their unique biology. The stress response involves the adrenal glands, along with the hypothalamus and pituitary glands, collectively known as the HPA axis. When a stressor is perceived, whether real or imagined, the hypothalamus releases a hormone, (corticotrophin), which tells the pituitary to release another hormone (adrenocorticotrophic hormone) which then signals the adrenal gland to produce cortisol (and DHEA). The cortisol then sends a message back to the pituitary and hypothalamus to inform the HPA axis that the cortisol has been made and there is no need to keep making more of it. That is the simplified version. However, when one is continuously and chronically stressed, that natural feedback system can be altered and more cortisol keeps being produced. Unchecked, the cortisol can then lead to the many health consequences listed above.
Living in the chronic state of exhaustion, along with other stress related symptoms, is not what women had in mind for life after 50. This is the time meant for enjoying the fruits of a lifetime of work and accomplishments, time for travel, family, grandchildren, culture, new interest, and personal growth.
"Each moment of worry, anxiety or stress represents lack of faith in miracles, for they never cease."
T.F. Hodge, From Within I Rise: Spiritual Triumph Over Death and Conscious Encounters with "The Divine Presence"
The solutions to chronic stress response and its accompanying health issues are as individual as each person. In today's world we must get to the specific underlying causes of hormonal imbalances, which may include nervous system interference, lifestyle issues, nutritional deficiencies, environmental toxins, digestive issues including food intolerances and leaky gut syndrome, immune challenges, as well as underlying emotional patterns.
"It was after I first began to uplift my thoughts a bit that my cravings for junk food started to dissipate. I did not connect the two at the time. First, I simply noticed that I didn't need to sleep so much. It took a while before I realized that in addition to my improved energy level, there was a direct correlation between chewing on mental garbage and putting garbage in my mouth."
Holly Mosier
You may not be able to remove all the stresses from your life but there are some steps to help minimize their impact on you:
First and foremost: make a decision to do whatever is necessary to correct this. It all starts with you.
Eat a healthy, whole foods diet.
Keep your carb count between 70 and 100 grams per day, even if you are vegan or vegetarian. Most women thrive at this level.
Increase your intake of healthy fats: flax oil, coconut oil, hemp seed oil, olive oil, olives, full-fat plain yogurt, raw dairy, raw cheeses, avocados, butter, eggs, cod liver oil, almond butter, canned sardines, grass-fed butter.
Avoid excess coffee and alcohol.
Avoid/decrease sugar consumption… it will sabotage all your other efforts. Become an expert on reading labels.
Avoid genetically modified foods.
Get plenty of quality sleep.
Exercise regularly. Even going for a walk around the block helps.
Relaxation techniques (visualizations, meditation, prayer, yoga, tai chi, chi gong, etc.)
Spend time in nature.
Spend time with family and friends.
Neutralize or minimize electromagnetic frequency exposure.
Speak your truth, live your truth (find and do what interests you).
Get professional help when needed.
"I am a very old man and have suffered a great many misfortunes, most of which never happen."
Mark Twain
Unlike our ancient ancestors, we can't run away from the threats we face today, therefore we must take charge of our own health and that of our families. Not only can we overcome the effects of chronic stress, we can even thrive in a life of unlimited possibilities.
Dr. Donald Piccoli is a chiropractor certified in advanced Nutrition Response Testing and is the director of Holistic Solutions in Kensington, CT.
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Plumber Murray
Tips That Guarantee The Best Plumber Murray
Sometimes plumbing emergencies do occur and in such scenarios, we may tend to be stressful and frustrated. However, these should not let us hire anybody available to make such repairs. The following are tips to guide you into choosing the best plumber Murray;
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The more working duration the more the experience. One should hire the most experienced plumber since they can successfully locate and fix any plumbing mistakes. Moreover, they provide better services since they are used in doing such tasks.
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If you can successfully source for a recommendation from friends, relatives and other people you are likely to find the best plumber Murray. So don't shy off from consulting from the people around you, since perhaps they have received the services of a plumber one time or the other.
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It is recommended that one should choose the plumber Murray who is near his/her locality. This is to minimize the cost to be involved and most importantly to ensure you can conveniently call them in case you have any plumbing emergency.
The above tips will go a long way to help you secure the best plumber Murray worthy your money and save you a lot of time.…


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No Job Is Too Big Or Too Small!
Whatever your outdoor lifestyle requirements might be, Welch Construction is constantly prepared to try harder for any kind of task. Next is a full listing of the Bridges job we would execute for you in Bryan Texas:
- Attractive Lanais and Pathways - We manage concrete, paving stones, and adobe and concrete paving stones.
- Assorted Classes of Holding Partitions – Wood natural stone, timber, dry-stacked unfinished stone and boulders, and fence systems made on cement blocks.
- Woodworking - Steps, lanai, spans and pathways; diverse types of arborsand pavilions; the staining or repair of fencing and decks; lakefront decks and piers; patio enhancements such as flowerpots, seats and handrails; outside home servicing, renewal, and addition of shingles, trim, garages, and carports.
- Barricades - Work on many types of wooden backyard fencing like trellises, porch screens, game, pig, and equine.
- Drainage – Cut and fill and run-off efforts to handle runoff; discharge covers and placing of pipe; numerous drains, including line and blind; upgrading rainwater-only stream beds to take care of flash flood overflowing.
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An inhabitant the Brazos Valley for four decades, the the person who owns Welch Construction has knowledge in the neighborhood and methods to handle any Bridges outdoor projects in the list above. With over three decades of practical familiarity, a representative with Welch Construction will discuss with you in-person and review your outdoor development preferences near Bryan Texas that can involve Bridges. The consultation is without charge and shall contain a quote of the price for your undertaking.


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Caylee Southland is Civilian's talented web content coordinator for all things digital. She is equally comfortable crafting awesome social media content, running large web projects, or polishing off a huge california burrito all by herself.
When you were 10 years old, what did you want to be when you grew up?
A lawyer
A social cause you care deeply about and why?
Climate change, because not much else will matter if the planet becomes uninhabitable.
If you could earn a living doing anything else, what would it be?
A food critic, aka get paid to travel and eat.
You can invite 3 people from either present day or history to break bread with, who would it be and why?
Alex Honnold – I have so many questions for him.
Miley Cyrus – I think we would be best friends. lol
Anthony Bourdain – he'd have the best stories.
In 5 words, describe yourself? (must use all 5….no more…no less)
Did someone say breakfast burritos?


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Chances are you're here because you're right in the middle of planning your wedding you already have a ton on your plate. I totally get it and I'm here to help.
I'm Meg Russell, a wedding and lifestyle photographer and I love to help couples create beautiful and timeless photographs.
That's why I've created a helpful guide to help you set yourself up for a stress-free experience. I hope this helps in planning your big day!
Please download the guide below and keep in touch! Let me know if this has helped you or if you have any other questions about wedding photography!


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Jobs 1 - 10 of 299
QA TesterFiresoft ConsultingSydney CBD NSW$70,000 - $82,000 a yearQA Tester (Winforms expereince essential) Located in Sydney CBD $70k - $80k + super Opportunity for career growth Our team is comprised of a dedicated team...Missing:
QA Analyst - cloud specialistCloud ConformitySydney NSW$85,000 - $115,000 a yearThe QA Analyst - cloud specialist, will join the Conformity team in Sydney, Australia, and be responsible for providing quality assurance for the Conformity...
Head of QA-newCanvaSydney NSWWe're looking for a candidate to fill this position in an exciting company in The Insurance Sector. Coaching the QA engineering team on quality assistance...Missing:
Head of QA-newCanvaAustraliaWe're looking for a candidate to fill this position in an exciting company in The IT Sector. Be responsible for growing and developing the QA division,...Missing:
Test Manager-newCubic CorporationSydney NSWWe're looking for a candidate to this position in an exciting company in The IT Sector. Manage, coordinate and oversee the work of the test team and ensure...Missing:
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Senior Dev Test Engineer-newINGSydney NSWIt's an exciting time here at ING and we are looking for a Senior Test Development Engineer to develop the ING test automation practice, while balancing...Missing:
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Automation TesterTalenzaSt Leonards Park NSW$120,000 - $130,000 a yearAs the Automation Engineer you will be expected to build test automation frameworks from scratch leading from the front. Back and Front end testing will be...Missing:
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Test ManagerTalenzaSydney NSWThis leading financial services group finds itself extremely well positioned in the current climate and is currently looking to appoint an energetic and high...Missing:
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Automation Test AnalystRobert HalfMacquarie Park NSW$110,000 - $125,000 a yearThis National client is currently seeking a talented Automation Test Analyst to join a high performing team based in Macquarie Park. You will be responsible...Missing:
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Discover which test is best suited to your symptoms by taking our free, 2-minute self assessment.Free Self Assessment
Your blood sample will be analysed for elevated IgG antibodies toward up to 270 foods.About the Test
ImuPro offer a variety of test packages for many health concerns.Compare the Tests
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In-depth medical knowledge and scientific support are extremely important for constantly improving a diagnostic tool like ImuPro. Therefore, in 2013 our International ImuPro Scientific Advisory Board was founded: a committee of medical doctors and scientists from seven countries worldwide. Find out more.
Crohn's Disease (CD) is a chronic inflammatory condition commonly affecting individual sections of the gastrointestinal tract; most often the transition of the small intestine to...Continue to the full story
Jennifer – Perth, WA Hi Cassie, I've been meaning to email you to let you know about my experience with the ImuPro test. I have...Continue to the full story
Psoriasis – an inflammatory skin disease Psoriasis is a skin disease that causes raised, red, scaly patches to appear on the skin. The skin cells...Continue to the full story
Jennifer – Perth, WA Hi Cassie, I've been meaning to email you to let you know about my experience with the ImuPro test. I have had inflammatory arthritis for nearly 20 years and have been taking biologic drugs for about 15 years. For the last couple of years I've struggled with the effectiveness of these... Read more »
Jemma and her son Kai, 5 years old – New Zealand I have 4 young boys, 3 of which have varying degrees of eczema. We had tried everything from creams to gut healing to Karen Fischer eczema diet, gluten free, dairy free, supplements, probiotics- EVERYTHING! My 4 year old son Kai's eczema was becoming so... Read more »


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If you signed up for lunch pick up or delivery, please see the lunch menu. READ MORE >
Staff: On Monday night, the LCSC Board of Trustees approved the attached Resolution. You may watch the board meeting in its entirety at the following link; https://youtu.be/jSx3BKRJtP0. You can view the resolution at this link; https://drive.google.com/file/... In summary, this means the school corporation will continue paying staff their full regular amount of... READ MORE >
The Lawrenceburg Community School Corporation issued this latest parent letter to keep families and the community informed. Please click on "Coronavirus Information" above for the letter. READ MORE >
Lawrenceburg Primary School has launched a Dual Language Immersion program in kindergarten and first grade. Students spend half of their day learning the Indiana College and Career Readiness Standards in English and the other half in Spanish. READ MORE >
Lawrenceburg Community School Corporation has made our Bus Replacement Plan and Capital Project Plan available to the public. Please click on the link to learn more. https://drive.google.com/file/... READ MORE >
The Lawrenceburg Police Department and Lawrenceburg Community School Corporation came together to make a video to help parents and students recognize what being a bully means and how to recognize signs that your child is being bullied or is being a bully. LPD Bullying Video READ MORE >
Annual Performance Report. Lawrenceburg Community School Corporation publishes it's Performance Report every year. Please click on the link to view the report for the 2018-2019 school year, READ MORE >
Lawrenceburg Community School Corporation is now working with SafeSchools, a school safety and compliance program, created by Vector Solutions. The SafeSchools suite includes a staff training system, an incident tracking system, an SDS and chemical management system, and a tip reporting system. We want everyone at Lawrenceburg Community School Corporation to feel... READ MORE >
Greendale Middle School recognized as a Common Sense School Lawrenceburg, IN – Common Sense, the national nonprofit organization dedicated to helping kids and families thrive in a world of media and technology, has recognized Greendale Middle School as a Common Sense School. Greendale Middle School has demonstrated its commitment to taking a whole-community... READ MORE >
To create a new article, click the "Add New" button. A menu will appear on the right side of the screen allowing you to add content to your news article. 1) The cover photo is an optional photo that appears above the text of your article. 2) Article title is a required field. This is the title of the article that will appear in the article as well as on your... READ MORE >


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9 Reasons You Should Join the Free Goal Minded Teacher Online Course!
Teacher Reboot Camp
Get your copy of Hacking Digital Learning , The 30 Goals Challenge , or Learning to Go. Earn a badge for your efforts, which is backed by INTEF and the Ministry of Education in Spain! "The whole being of any teacher is within our hearts.


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About STEM Central
Welcome to STEM-Central.net: Making Connections, Making a Difference in Undergraduate STEM Education.
STEM Central provides a forum for STEM faculty and administrators nation wide to connect with others and learn, share ideas, share resources, and work collaboratively on initiatives.
Previously STEP Central, who's audience was primarily NSF-STEP Grantees, STEM Central's audience is much broader and includes all STEM faulty and administrators who strive to improve Undergraduate STEM Education.
We are currently working with leaders in the community to create appropriate working groups and add resources that are relevant to our broader audience. We invite your ideas and suggestions. Please contact us and let us know how we can make this site work better for you.


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Welcome to KenricoCare. KenricoCare has been provided for over 30 years and is supported by our licensed medical doctors or physicians at Kenrico. We are here to
answer any of your health-related questions and to help you with our suggestions based on our expertise, knowledge & experience. Please refer to the posting guidelines before posting.
- Next topic: Salmonella contaminated foot pads - Started by Jamal December 19, 2019 at 01:40:35 AM
- Previous topic: Diarrhea after using Chinese pads - Started by Ben December 17, 2019 at 11:18:35 PM
Re(1): Terrible headaches
All brands sold in Amazon or Ebay that do not state Kenrico as their manufacturer, are coming from a counterfeiter in China. They have been reported to have been contaminated with salmonella, bacteria and harmful chemicals.
The headaches are the result of chemical poisoning. Typical symptoms of chemical poisoning are headaches, nauseas, vomiting, and dizziness.
Please discard the use immediately and follow the protocol using Kenrico pads:
|KX-2 4000 mg and TRMX 4 ORIGINAL 8500 mg
|LEXIRIN 8500 mg and TRMX 4 ORIGINAL 8500 mg
|ZEO 5000 mg and TRMX 4 ORIGINAL 8500 mg
|QU-1 4000 mg and AQUA COOL TUV 5000 mg
|KX-2 4000 mg and TRMX 4 ORIGINAL 8500 mg
|LEXIRIN 8500 mg and TRMX 4 ORIGINAL 8500 mg
|ZEO 5000 mg and TRMX 4 ORIGINAL 8500 mg
Daily dosage is two (2) pads per day every day.
The 3D foot reflexology chart is now available for both PC, iPhone / iPad and Android:
iPhone / iPad
Placement areas on the feet according to the 3D reflexology chart at https://www.kenrico.com/kenrico_reflexology.html:
- TRMX 4 ORIGINAL 8500 mg
- Brain stem / cerebellum / head, liver, lymph glands
- KX-2 4000 mg
- Cerebrum
- ZEO 5000 mg
- Kidney, spine
- LEXIRIN 8500 mg
- Kidney, spine
- QU-1 4000 mg
- Liver
- TUV 5000 mg
- Stomach, ascending large intestine
|GREEN EDITION KX-2 4000 mg
|KX-2 with SPOROLIFE is intended only for neurological conditions that require attentive treatment and suitable for patients undergoing therapies or rehabilitation. Made in Japan.
|SUPER DETOX EDITION ZEO 5000 mg
|Super Detox Edition ZEO with SPOROLIFE and Zeolite has rejuvenation effects to repair damaged cells in the organs. Made in Japan.
|QUICK EDITION QU-1 4000 mg
|QU-1 contains SPOROLIFE and Activated Milk Thistle for treating liver infection, bile obstruction, gallstones and other dysfunction from substance abuse.
|SOOTHING EDITION AQUA COOL TUV 5000 mg
|Aqua Cool TUV with SPOROLIFE emits cool soothing effects for curing digestive problems, excretory problems and heatstroke. Made in Japan.
All the best,
Thread overview
Terrible headaches - By Jessi December 19, 2019 at 01:33:45 AM
- Re(1): Terrible headaches - By KenricoCare December 19, 2019 at 01:48:40 AM
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You can't reply to this message because this message is too old.
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Ahead of the 2022 FIFA Wolrd Cup tournament in Qatar, the governing body FIFA has now confirmed that they are investigating a possible match-fixing offense in one of the qualifiers match for Group F.
The game they are looking into is Myanmar's 7-0 loss to Kyrgyzstan in an away match in October 2019, which was a third straight loss for Myanmar who had been beaten by Mongolia and Japan in the other group clashes.
"We are looking into this matter," FIFA said, confirming reports in Myanmar media which said officials had visited the country to question players.
"Anyone found guilty of match manipulation will be subject to severe sanctions as FIFA has recently introduced minimum bans of five years," the soccer's world body added in a statement.
Myanmar next plays group leader Japan and Kyrgyzstan in March.
The top two nations in the five-team group will advance to the next stage of qualifying for the 2022 tournament in Qatar.
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Paul Martin and David L. Cockroft
A major disadvantage of working with postimplantation mammalian embryos is their relative inaccessibility to experimentation while they develop within the maternal uterus. Two techniques allow us to get around this problem to a large extent. The first, which is the subject of this chapter, can be used for mouse embryos explanted between 7.5 d of gestation (E7) and 12.5 d of gestation (E12), and involves dissecting embryos from the uterus and culturing them in roller bottles (1). In this way, embryos can be surgically or chemically manipulated or labeled and will develop quite normally in culture for periods of 12-60 h, depending on the stage at explantation (2,3). The second technique, which allows experimentation on more advanced stages, is that of exo utero or open uterus surgery, in which fetuses are suspended in the fluid-filled abdominal cavity of the female mouse while retaining their placental attachment to the uterine wall (4). This procedure is suitable for fetuses at 12.5 d of gestation (E12) and beyond. This chapter will focus on the techniques for culturing E11 mouse embryos with open yolk sacs at limb-bud stages (5) and will also include protocols for culturing earlier stage embryos. We will describe studies in the mouse, but similar manipulations are possible with rat embryos, bearing in mind that they are generally 1 or 2 d behind mouse development; for example, an E11 rat embryo closely resembles a mouse embryo between E9 and E10.
1. Microscope: A good-quality dissecting microscope is essential, preferably with both transmitted (bright- and dark-field) and incident (e.g., fiber-optic) illumination—we use Wild microscopes with an overall magnification range of 6-50x.
2. Instruments: Table 1 shows the tools you will need and what they should be used for.
From: Methods in Molecular Biology, Vol. 97: Molecular Embryology: Methods and Protocols Edited by: P. T. Sharpe and I. Mason © Humana Press Inc., Totowa, NJ
Table 1
Tools for the Job
1 Pair coarse scissors, 1 pair coarse forceps
2 Pairs fine serrated, or watchmaker forceps 2 Pairs watchmaker's forceps (no. 5), carefully ground to a tip diameter of 0.05-0.1 mm (see Note 1) Iridectomy scissors (large)
Iridectomy scissors
(small) Hemostat (Spencer-Wells forceps)
Opening the abdominal cavity, when explanting embryos or bleeding rats Removing uterus Removing uterus, opening uterus, exposing aorta during bleeding Removing decidua of E7-E9 embryos, opening Reichert's membrane
Removing decidua/Reichert's membrane of E10-E11 embryos Opening yolk sac of E10-Ell embryos Holding colon out of the way during bleeding
Can also be used for removing uterus
Do not use for opening uterus and so on, since they are too delicate and easily damaged by such heavy work Can use watchmaker's forceps instead
3. Dishes: 5-cm plastic Petri dishes (Sterilin) are suitable for all the operations of explanting embryos at all ages described here. You will need 3-6/litter (more for older embryos); always transfer embryos to a fresh dish if the medium becomes cloudy with blood, and so forth, and ensure that dishes contain enough medium to submerge embryos completely.
4. Saline recipes—see Table 2—we use a 1:1 mix of Tyrode's and Earle's salts (=explanting saline) for explanting open yolk sac embryos. To this, we add extra bicarbonate and glucose (=culture saline) when it is used in the culture medium, in order to equilibrate it with the CO2 in the gas mixture used during culture and to increase the energy sources.
Embryos cultured with closed yolk sacs are explanted in PB1, and cultured in pure rat serum. The pH of all media should be about 7.2.
5. Rat serum: This is the basic culture medium for all postimplantation embryo culture, and is used undiluted for embryos cultured with closed yolk sacs (E7-E10 mouse) (6), or diluted to 25% for culture with open yolk sacs (E10-E11 mouse) (5). Rat serum is available commercially, at a price, but because of the method of preparation, it is inferior, particularly for the culture of the earlier stages (7,8), to what you can prepare yourself (see Subheading 3.1.3.).
Table 2 Media
PB1 medium,
saline, g/L
saline, g/L
Na pyruvate
Fetal calf serum
10% v/v
6. Sterility: Provided all dishes, instruments, and media used are sterile, we find that explantations and experimental manipulations can be performed on the open bench without problems of infection.
3. Methods 3.1. General
The best way to obtain embryos at precisely the stage of development needed is to oversee the mouse mating procedure yourself. If only small numbers of litters are required (1-10 litters/wk), then a colony of 5 studs, with virgin females bought in as needed (say 20 females/2 wk) is quite satisfactory. For larger numbers of litters, it is probably more economical to set up your own breeding colony, with weaned newborns grown up to replace the female stock. We usually mate female mice at 8-12 wk old, and retire our breeding studs before they are a year old. With albino varieties of mice, it is relatively easy to detect the one day in the cycle of 4 or 5 d that females are in estrus, because the vagina appears pink and swollen by comparison with nonestrus females. In nonalbino strains it is harder to distinguish estrus, and if this cannot be judged, then three or four times as many breeding pairs should be set up to make allowance for the nonestrus females. We introduce females individually to studs late in the afternoon, and check for vaginal plugs the following morning. The hard whitish plug occluding the vagina is usually very obvious, but occasionally it is located deep, and this can be checked using a round-ended probe. If you have too few studs for paired matings, then it is possible to put up to three females with one stud, but plugging efficiency and litter number will sometimes be compromised. Generally, we expect 30-80% of our females judged to be in estrus to become pregnant. Depending on strain of mouse, the litters will contain from 5-15 embryos.
In the United Kingdom there are "Home Office" (HO) regulations stipulating methods of killing animals. The most satisfactory HO Schedule 1 method for killing a pregnant mouse is by cervical dislocation. This is done humanely by placing the fingers of one hand behind the animal's neck and stretching it by pulling the mouse's tail with your other hand. Alternative methods of killing include overdoses of anesthetic, but this may have adverse effects on the later health of the embryos.
Rats (preferably fasted overnight) should be anesthetized with halothane or (if permitted) ether. Lay the anesthetized animal on its back, douse it with 70% ethanol, and open the abdominal cavity. Displace the small intestines to the left, pull the colon to the right, and hold it there with a hemostat (Spencer-Wells forceps). Expose the dorsal aorta with fine forceps (it is generally smaller and paler than the adjacent vein), and insert, bevel down, a 19- or 21-gage needle connected to a 10- or 20-mL syringe, with all air excluded. Gently withdraw blood until the rat stops breathing, after which only a further milliliter or two can be obtained. Remove the needle and transfer the blood to a 12- or 15-mL centrifuge tube, running it gently down the side of the tube. Immediately spin the tube for at least 5 min at 2000g. This will precipitate the blood cells, and a whitish clot will form in the supernatant serum. When all rats have been bled (20-30 is a reasonable number for a single session), squeeze the clots with long slender forceps to expel serum, spin again as above, and decant the serum into 50-ml centrifuge tubes with a pipet. Spin again to bring down any persisting blood cells, and then pool the day's serum in a suitable container (e.g., a 250-mL tissue-culture flask). Add antibiotics if desired (e.g., 100 |g/mL Streptomycin, 100 IU/mL penicillin), and aliquot the serum in quantities of 5-20 mL before freezing. It will keep for several months at -20°C, or years at -70°C (freeze first at -20°C, and then transfer to -70°C; otherwise tubes may crack). See refs. 3 and 9 for further information on rat bleeding.
Thaw the serum at room temperature or in a 37°C water bath, then heat-inactivate it for 30 min at 56°C. Gassing the serum while hot will help drive off any persisting anesthetic dissolved in it. A residual clot often appears at this stage, and can be removed by centrifugation or, more effectively, by filtration through a 0.45-^m syringe filter (Sigma). Serum is then ready for use, either pure or diluted, according to embryonic age.
3.2. Explanting E11 Embryos (Cultured with Opened Yolk Sacs)
1. Although the procedure for explanting with open yolk sacs described and illustrated here is for E11 embryos, it is equally applicable to E10 embryos. The freshly killed pregnant animal is laid on its back and the abdomen doused with 70% alcohol. The abdominal skin can be pinched between thumb and forefinger of both hands, and the skin torn back towards head and tail to reveal a clean peritoneal surface, which can then be opened with scissors and forceps. This method avoids contaminating the abdominal cavity with hair. Alternatively, all abdominal layers can be opened at once with a large U-shaped scissor incision, with the two ends of the U at the hindlimbs.
2. The uterus with embryos is then lifted clear of the abdominal cavity. It can be held with forceps midway along one horn at a site between two embryos and needs to be severed with scissors at the tip of that horn (near the ovary), then where it communicates with the cervix (without separating the two horns), and finally at the tip of the second horn. As this is being done, the uterus can also be trimmed free of mesentery and fat.
3. The whole uterus should then be rinsed in PBS and remaining mesentery cleared away before transfer to a fresh dish of PBS. The uterus can now be opened to expose the embryos. This is done by carefully tearing along the antimesometrial wall of the uterus with fine forceps (Fig. 1). It is easy to damage embryos at this stage, and the best way to avoid this is to keep the two pairs of forceps close to one another, so that as they tug apart, all the effort goes into tearing the uterus and not squashing the embryos. Once the uterus is open, the embryos (concep-tuses) appear like peas in a pod attached to the uterus only in their placental regions. The easiest way to free this attachment is to hold the uterus tightly with one pair of forceps, slip the other pair of forceps on either side of the uterine sheet, and then drag them across each placental attachment in turn, gently teasing the embryos free (Fig. 2).
4. The individual conceptuses can now be transferred by pipet to a fresh dish of explanting saline, and their decidua removed. The decidua of an E11 embryo is thin and is best peeled off, beginning on the side opposite the placenta, by shallow pinching with two pairs of fine forceps, which allows a gentle tearing action. Often the very thin and transparent Reichert's membrane, which sits beneath the decidua and clings to the yolk sac, will rupture during this operation, in which case it is easy to remove it with the decidua. When the decidua has been torn back to about the level of the placenta, it can be tidied up by trimming off with a pair of iridectomy scissors (Fig. 3). If Reichert's membrane is still intact, it must be torn open and trimmed back to the placenta also.
5. Next, the yolk sac must be cut with fine iridectomy scissors, close to where it abuts the placenta, taking care to avoid damaging any of the larger yolk sac vessels (Fig. 4). First use two pairs of watchmaker's forceps to make a small hole in the yolk sac adjacent to the placenta, to allow access for the iridectomy scissors. The yolk sac should not be cut completely free of the placenta—rather cut about 4/5 of the way around, creating enough of an opening for the embryo to be pulled out of the yolk sac head first. The region
Fig. 4. A small hole is made in the yolk sac close to where it abuts the placenta and adjacent to the forelimb bud. Iridectomy scissors are then used to cut around 4/5 of the base of the yolk sac.
of yolk sac left uncut should be that adjacent to the tail of the embryo— otherwise delivery is made significantly more difficult. The embryo is drawn out of the yolk sac by pulling on the amniotic membrane overlying the embryo's head with one pair of forceps, while holding the mouth of the yolk-sac incision with a second pair of forceps (Fig. 5). After the head is outside the yolk sac, it is necessary to rupture the amniotic membrane in order to exteriorize the rest of the embryo. Last of all, the yolk sac and amniotic membranes are flipped under the tail, and the embryo is now available for manipulations or immediate culture.
Fig. 4. A small hole is made in the yolk sac close to where it abuts the placenta and adjacent to the forelimb bud. Iridectomy scissors are then used to cut around 4/5 of the base of the yolk sac.
6. If the developmental stage of the embryos is critical for your experiment, you should stage them now (see Note 2).
7. All of the above procedures and any planned subsequent manipulations (see Note 5) should take no more than about 2 h, or subsequent development in culture will be compromised. This generally imposes a limit of no more than 2 litters of embryos/culture session. When embryos are ready for culture, they are individually transferred into 50-mL Falcon tubes containing 5 mL of 25% (v/v) rat serum in culture saline (see Table 2 for recipes). To ensure a gaseous seal, we apply a thin coat of silicone vacuum grease (Dow-Corning) to the rim of the tube. The tube is then gassed with 95% O2, 5% CO2 (for 1 min at a gas flow rate sufficient to ruffle the surface of the medium gently—Fig. 6). The Falcon lid is then screwed tight and the tube is placed in a 37°C incubator containing rollers-rotating at 30 rpm. We find that the custom-made BTC roller-incubators (see Note 9) are excellent for this purpose. By carefully stacking Falcon tubes in the incubator, it is possible to culture up to 18 tubes (embryos) at one time.
8. The health of embryos can be determined at any stage during culture by brief removal of the Falcon tube from the incubator and viewing under a dissecting microscope with transmitted light. Because the walls of the tube are translucent, it is easy to check how well the heart is beating, and usually whether there is good circulation in the larger vessels of the yolk sac and over the brain. Also, check whether there is any swelling of the pericardium or blistering of distal limb ectoderm (usually signs that the embryo is faring badly). In any case, embryos should be regassed every 12 h or so. Only badly damaged embryos will fail to culture successfully for 12 h and, of these, 90% will successfully make it through to 24 h: From this stage on, survival rates get steadily worse with only 1 in 3 embryos
healthy at 36 h and 1 in 6 or 7 making it through to 48 h. Figure 7A shows the appearance of E11 embryos before and after culture for 24 h.
Explanting E9 Embryos (Cultured with Closed Yolk Sacs)
Embryos younger than E10 at explantation are cultured with the visceral yolk sac intact. The initial stages of killing the mouse, opening the uterus, and separating the conceptuses are the same as for the E11 embryos, though of course the con-ceptuses are smaller.
Removal of the decidua of E9 embryos is similar to the E11 procedure, since it already forms a relatively thin layer over the conceptus. It is advisable to remove all of the decidua, starting at the equator and tearing toward and over the placenta, but being careful not to damage the latter, since it has an extensive blood circulation at this stage.
Next, open Reichert's membrane; although this is thin and transparent, it is mostly overlain with a layer of trophoblast and blood cells, and it may be necessary to pick through this layer before rupturing Reichert's membrane, after which removal is straightforward, trimming it up to, but not beyond, the placental border (Fig. 8).
4. If the visceral yolk sac or placenta is damaged, causing deflation or bleeding, the embryo should be discarded; otherwise, it is ready for culture.
5. E9 embryos may be cultured in 50-mL Falcon tubes as above or 30-mL Universal containers (Nunc, with a smear of silicone grease round the internal rim of the lid to provide a gas-tight seal) with 1-1.5 mL pure serum/embryo, and 2-5 embryos/ tube, depending on size. Initially, the embryos should be gassed with 5% CO2 in air (i.e., 20% O2, 75% N2, 5% CO2), which is replaced with 40% O2, 55% N2, 5% CO2 after 18-24 h of culture. Figure 7B shows the extent of development of E9 embryos after 48 h in culture, though 18-24 h are a more realistic period if you need the majority of embryos to be healthy at termination.
3.4. Explanting E7 Embryos (Cultured with Closed Yolk Sacs)
1. The initial stages are as above, but with E7 embryos, removal of the decidua requires a rather different strategy, since the conceptus is embedded in a relatively thick mass of decidua. Start by impaling the decidual mass parallel with its long axis, but off-center, with one arm of a sharpened slender pair of forceps. Then squeeze the forceps together to form an incision in the decidua.
2. Repeat on the other side of the decidua, then unite the two incisions around the base of the conceptus (usually the thicker end).
3. Now grasp the two flaps of decidua with two pairs of watchmaker forceps, and pull apart (Fig. 9), when the conceptus should be exposed, and usually it will
Fig. 7. (continued) The embryo on the left was refrigerated at explantation; on the right is a littermate cultured for 48 h. The embryos are shown dissected free of the extraembryonic membranes with which they are cultured. (C) E7 embryos before and after culture. An E7 conceptus (including the visceral yolk sac and ectoplacental cone with, which it would be cultured) is shown on the left. This was refrigerated while littermates (center—with membranes as cultured; right—dissected free of membranes) were cultured for 48 h. Scale bars for A, B, and C are all 1 mm.
remain on one of the decidual halves as they are separated. If the embryo sticks to both decidual halves, so that separating them further might damage it, either hold the flaps apart with one pair of forceps, whilst teasing the embryo free on one side with the apposed tips of the other pair of forceps, or make a further incision in one of the decidual halves, so that only a quarter is removed initially, followed by the second quarter.
4. Once this has been accomplished, further divide the base of the decidua containing the embryo along the long axis, and peel apart along the length of the conceptus (Fig. 10). Repeat if necessary until the embryo is attached only to a thin sliver of decidua, like a segment of an orange.
5. Impale the sliver of decidua on either side of the embryo with the points of one pair of forceps, stretching it slightly, and tease the embryo free with the apposed tips of the other pair of forceps (Fig. 11).
6. All that remains is to open Reichert's membrane (Fig. 12); as with the E9 embryos, this may be overlaid with blood and trophoblast, though the membrane itself is thin and transparent. Sometimes Reichert's membrane stands clear over the embryo (at the end opposite the reddish ectoplacental cone), where it can be grasped and pulled apart. Otherwise, it will be necessary to use two pairs of watchmaker forceps to rupture the membrane midway along the length of the conceptus, in a region overlying the visceral yolk sac, where unseen damage to the underlying tissue will be less serious than damage to the embryo itself. Once opened, Reichert's membrane is trimmed off to the border of the ectoplacental cone, which is left intact.
7. The E7 embryo is now ready for culture. These embryos are cultured with 1 mL pure serum/embryo, with three embryos per 30-mL Nunc tube, or up to five embryos per 50-mL Falcon tube. They are gassed initially with 5% O2, 90% N2, 5% CO2, and then with 5% CO2 in air (i.e., 20% O2) after 24-36 h of culture (i.e.,
Fig. 11. The conceptus is teased free from a narrow sliver of decidua.
Fig. 11. The conceptus is teased free from a narrow sliver of decidua.
Reichert's membrane is opened with finely ground watchmaker's forceps.
when the heart beat and visceral yolk sac circulation are established). Figure 7C shows the extent of development of E7 embryos after 48 h in culture.
1. Sharpening forceps: A combination Japanese waterstone (1000/6000 grit, e.g., King brand) is ideal. Wet it with distilled water and grind the forceps along their length until a tip diameter of 0.05-0.1 mm is achieved. It is very important that the tips meet precisely, without crossing or one protruding beyond the other. Then round off all but the innermost (mating) edges, so that when apposed, the tips together form a blunt-ended probe.
2. Within and between litters of embryos of the same age, the range of developmental stages can vary dramatically. There are a number of established staging guides: ref. 10 covers all stages from fertilization to birth, ref. 11 from fertilization to 4 wk postpartum, ref. 12 from 8-16 d, ref. 13 from 7.5-10.5 d, ref. 14 from 6.75-8.0 d, and ref. 15 from 9 d to post natal stages. Between E9 and E14 a useful method of accurately staging embryos is to use the shape of the fore- and hindlimbs (16). These publications will also be useful in assessing the stage of development reached after culture and how it compares with growth in vivo. References 10 and 12 also provide a wealth of additional information, including sectioned embryos, which can help determine the success of your experiment.
3. An alternative method of transferring embryos from dish to dish while they are surrounded by their decidua is to pick them up with watchmaker forceps where the decidua is thickest, thus minimizing carryover of medium from one dish to another.
4. When the time comes to harvest your cultured embryos, they should be gently slipped into ice-cold PBS, and trimmed free of their yolk sac and placenta by cutting the umbilical vessels. Embryos can then be transferred to the fixative of your choice. We favor half-strength Karnovsky's fixative (17) if embryos are to be subsequently processed for scanning electron microscopy or resin histology, or 4% paraformaldehyde if they are for whole-mount or sectioned immunocy-tochemistry or in situ hybridization studies. For general wax histology, good results are obtained with Bouin's fixative followed by staining with Hematoxylin and Eosin after sectioning (10).
5. Manipulations: We have successfully performed a number of manipulations on the E11 embryo after it has been delivered from its yolk sac and prior to culture. It is possible to inject reagents into various epithelial lumens—for example, we have injected the marker dye Monastral Blue into the otic vesicle (18). Similarly, it is possible to perform simple surgical operations (19-21) and to label groups of cells with the lipophilic dye, DiI (21). Even if the embryos bleed a little after surgical manipulations, they generally survive and can be successfully cultured. The two biggest problems with any such manipulations are being able to see well enough what you are doing and keeping the embryo from moving around the dish as you operate on it. The first of these problems is easily resolved with good lighting (both incident and transmitted) and the second requires gentle holding or supporting of the embryo with forceps.
6. Transgenic mice: It is possible to culture transgenic mice, but these will generally be derived from heterozygote crosses, so only a fraction of the embryos cultured from each litter will be of the required genotype. It is sometimes possible to take a small piece of tissue (tail tip or a small piece of yolk sac) at the time of explant, so that embryos can be genotyped by PCR during the culture period. Otherwise, this can be done with spare embryonic tissue taken after the culture period, but before fixation.
7. Synthetic medium: We have recently begun to culture E11 embryos with open yolk sacs in a serum-free medium made by Gibco BRL. This medium is excellent for E11 cultures up to 24 h, but we have not tried it for longer culture periods, or its efficacy for the more sensitive younger embryo stages. Good results can also be obtained over a range of stages with partially defined medium, made by extensively dialyzing rat serum, and then supplementing it with glucose, vitamins, and amino acids (22).
8. Adding glucose to serum: If you have fasted your rats before bleeding, they will have lowered blood glucose levels. Addition of 8 |L/mL of a 50 mg/mL stock solution of glucose to the serum will restore the glucose levels to that in serum obtained from fed rats.
9. Address for BTC: BTC Engineering, 12 Shirley Close, Milton, Cambridge CB4 4BG, UK.
We are particularly indebted to Catherine Haddon for the lucid diagrams illustrating the dissections. We also thank The Medical Research Council and the Wellcome Trust (P. M.), and the Imperial Cancer Research Fund (D. L. C.) for financial support.
1. New, D. A. T., Coppola, P. T., and Terry, S. (1973) Culture of explanted rat embryos in rotating tubes. J. Reprod. Fert. 35, 135-138.
2. New, D. A. T. (1978) Whole-embryo culture and the study of mammalian embryos during organogenesis. Biol. Rev. 53, 81-122.
3. Cockroft, D. L. (1990) Dissection and culture of postimplantation embryos, in Postimplantation Mammalian Embryos—A Practical Approach (Copp, A. J. and Cockroft, D. L., eds.), IRL, Oxford, pp. 15-40.
4. Muneoka, K., Wanek, N., Trevino, C., and Bryant, S. V. (1990) Exo utero surgery, in Postimplantation Mammalian Embryos—A Practical Approach (Copp, A. J. and Cockroft, D. L., eds.), IRL, Oxford, pp. 41-59.
5. Cockroft, D. L. (1973) Development in culture of rat foetuses explanted at 12.5 and 13.5 days of gestation. J. Embryol. Exp. Morph. 29, 473-483.
6. Sadler, T. W. and New, D. A. T. (1981) Culture of mouse embryos during neuru-lation. J. Embryol. Exp. Morph. 66, 109-116.
7. Steele, C. E. and New, D. A. T. (1974) Serum variants causing the formation of double hearts and other abnormalities in explanted rat embryos. J. Embryol. Exp. Morph. 31, 707-719.
8. New, D. A. T., Coppola, P. T., and Cockroft, D. L. (1976) Improved development of head-fold rat embryos in culture resulting from low oxygen and modifications of the culture serum. J. Reprod. Fert. 48, 219-222.
9. Freeman, S. J., Coakley, M. E., and Brown, N. A. (1987) Post-implantation embryo culture for studies of teratogenesis, in Biochemical Toxicology—A Practical Approach (Snell, K. and Mullock, B., eds.), IRL, Oxford, UK, pp. 83-107.
10. Kaufman, M. H. (1992) The Atlas of Mouse Development. Academic Press, London.
11. Theiler, K. (1989) The House Mouse. Atlas of Embryonic Development. Springer-Verlag, New York.
12. Rugh, R. (1990) The Mouse. Its Reproduction and Development. Oxford University Press, Oxford.
13. Brown, N. A. and Fabro, S. (1981) Quantitation of rat embryonic development in vitro: a morphological scoring system. Teratology 24, 65-78.
14. Downs, K. and Davies, T. (1993) Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope. Development 118, 1255-1266.
15. Wanek, N., Muneoka, K., Holler-Dinsmore, G., Burton, R., and Bryant, S. V. (1989) A staging system for mouse limb development. J. Exp. Zool. 249,41-49.
16. Martin, P. (1990) Tissue patterning in the developing mouse limb. Int. J. Dev. Biol. 34, 323-336.
17. Karnovsky, M. J. (1965) A formaldehyde-gluteraldehyde fixative of high osmo-larity for use in electron microscopy. J. Cell Biol. 27, 137,138.
18. Martin, P. and Swanson, G. (1993) Descriptive and experimental analysis of the epithelial remodellings that control semicircular canal formation in the developing mouse inner ear. Dev. Biol. 159, 549-558.
19. Martin, P. Dickson, M. C., Millan, F. A., and Akhurst, R. J. (1993) Rapid induction and clearance of TGFP1 is an early response to wounding in the mouse embryo. Dev. Genet. 14, 225-238.
20. Hopkinson-Woolley, J., Hughes, D. A., Gordon, S., and Martin, P. (1994) Macrophage recruitment during limb development and wound healing in the embryonic and foetal mouse. J. Cell Sci. 107, 1159-1167.
21. McCluskey, J. and Martin, P. (1995) Analysis of the tissue movements of embryonic wound healing—Dil studies in the wounded E11.5 mouse embryo. Dev. Biol. 170,102-114.
22. Cockroft, D. L. (1988) Changes with gestational age in the nutritional requirements of postimplantation rat embryos in culture. Teratology 38, 281-290.
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text: Tepper's Chest Hair 1,466 Posted February 2 2 hours ago, stirs said: Generally you get relief if a guy retires, but once Hurney does his thing with restructures, you get screwed. Live for today.... The major deadcap hits we have are because of Kalil (Hurney did not restructure) and Kuechly retiring. Kuechly had 2 restructures that were desperately needed. We were in cap hell, and no one had any inkling he was retire so soon. He freed up 5.1m in 2018 and 7.24m in 2019 to increase future cap hits by 1.8m each. Olsen retiring carries a smaller deadcap hit, while freeing up like 8 million. Come on numbers guy. 1 Quote Share this post Link to post Share on other sites


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IQS Directory provides a comprehensive list of miniature bearing manufacturers and suppliers. Use our website to review and source top miniature bearing manufacturers with roll over ads and detailed product descriptions. Find miniature bearing companies that can design, engineer, and manufacture miniature bearings to your companies specifications. Then contact the miniature bearing companies through our quick and easy request for quote form. Website links, company profile, locations, phone, product videos and product information is provided for each company. Access customer reviews and keep up to date with product new articles. Whether you are looking for manufacturers of miniature roller bearings, miniature ball bearings, miniature thrust bearings or customized miniature bearings of every type, this is the resource for you.
Del-Tron Precision, Inc. provides high quality and reasonably priced ball bearings and associated products such as roller bearings and thrust bearings. Del-Tron has maintained the same high level of service by continually developing new products that deliver important advantages to its customers. Contact us today to learn more!
TUSK Direct provides high quality linear motion components such as ball bearings at a quality and price that sets us apart from the competition. We offer same-day shipping, life-load calculators and CAD drawings are other services the team at TUSK is pleased to offer customers. Contact us before noon & you will be working with your products the next day. We want TUSK to be your number one source.
Isel has a variety of high quality patented ball bearings. We offer low cost and high performance linear motion, ball screws and patented anti-backlash ball nuts. Our linear motion components are manufactured and inspected exclusively in our own facility. Contact Isel today to learn more!
Alpine Bearing stocks over 3 million bearings & prides in distributing quality instrument ball bearings at guaranteed prices. Miniature bearings, thinex, torque tube, machine tool spindle, airframe control & turbo molecular pump bearings are available with fast delivery & expert re-lubrication service.
Whatever kind of bearings you're looking for, be it precision ball bearings, miniature bearings or discontinued items, Talon Bearing will have it. Our stock comes from all over the world. Buy as many or as few as you need, and if you have excess bearings, we'll pay cash to purchase them from you!
Miniature bearings are tools that are used to allow for relative motion
between two parts on a scale that is smaller than that provided by
bearings of larger sizes. Miniature bearings are put to use in systems
with comparatively small moving parts.
A bearing is any tool or set of tools that help parts move relative to each other. Industry, commerce and consumers rely on a wide variety of products that feature moving parts. Just a few examples of these products include air conditioners, automobiles, computer cooling fans, bicycles and even electric pencil sharpeners. In products that feature very small moving parts, the use of very small bearings is necessary. Miniature bearings are no different from traditional bearings except in terms of their small size and their ability to handle smaller torque loads. Like larger bearings, miniature bearings can be made of steel, ceramics and many other materials. They can be ball-shaped, cylindrical or shaped in other ways. They can be designed to be sealed or open. A few common uses for miniature ball bearings are in bicycle wheels, in pneumatic hose attachments, in remote control cars, in hand tools and in a variety of other products.
When two moving parts can roll over bearings instead of rubbing against each other, the amount of friction created is much smaller. This reduction in friction eases movement and reduces wear on the moving parts. The small balls, cylinders or other shapes in miniature bearings bear the weight of the load, which permits smooth movement. There are two classifications for bearings: linear and rotary, and this applies to miniature bearings as well as to bearings of other sizes. These classifications are based on the motions for which the bearings are designed. A linear miniature bearing moves in a straight line. These bearings are used for pushing and pulling motion, like that of a drawer opening and shutting. In a drawer system, miniature bearings can often be found along the length of the metal races that support the drawer. A rotary bearing, in comparison, is designed for radial motion, like that of a wheel spinning. Such bearings are often installed in bicycles to allow for smoother motion of wheels. Bearings of all sizes are essential tools in improving relative motion between all kinds of moving parts.


text: Day 24 Without Sports 😭: Here's how 2020 Final Four would have turned out 04/04/2020 7:07pm We project what might have happened in the two Final Four games that were supposed to be played Saturday in Atlanta. Author: HEDGEHedge Accordingly was founded ahead of the global financial crisis in January of 2008, with the goal of providing our readers our unique take on latest news on stocks & WallSt. Hedge Accordingly produces both original, and aggregated Wallst news content from top publishers around the world. We curate aggregated content covering the latest news on politics, stocks, wall street, and the tech industry. Click Here for Free stock charting, quotes and a bitcoin, forex and currency exchange. Learn More About Hedge Twitter Facebook


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Okay you've caught a great tasting fish or bagged a wild turkey and you are already thinking of how you'll prepare it either for grilling in the field or cooking back home. That's when you wish you had a Sous chef to help you come up with a delicious recipe and tempting presentation. The fans of the FishMate and HuntMate line of mobile apps for outdoors men asked their talented app designers-who are also great in the kitchen-to come up with an app for cooking their favorite fish and game, and have they created that, plus more exclusively for the iPad.
Interactive iPad app pre-loaded with over 650 recipes and more for just $2.99
ChefMate is an interactive App with over 650 pre-loaded recipes, more than 50 on grilling or preparing fish, steak, chicken, ribs, duck, game hens, squab, sausage and other meats along with shrimp, lobster, crab and shellfish. Does venison tenderloin sound appealing? There's more. How about Cajun catfish, almond crusted trout with Grand Marnier cream, bourbon marinated salmon, broiled salmon with pesto, citrus-grilled snapper with Mandarin orange relish, linguini in clam sauce, shrimp and snapper stew, grouper with pomegranate, grilled tuna and many other fresh and saltwater fish species?
ChefMate designer Penelope Fenton said, "The entrée section includes meats, poultry, seafood, vegetarian and pastas. There are also plenty of side dish selections from which to choose. "Cooking for more than a dozen?" Fenton asked, "ChefMate will quickly scale the ingredients to the proper amounts with just one click, so you don't need to do the math. Plus you get tips on substitute ingredients, onscreen fundamentals and kitchen tips.
ChefMate's "How to" section is alphabetized with topics on how to carve a turkey and a wide variety of meats, plus how to fillet a fish as well as larger fish into steaks. There are also more selections for
- Sauces, starters, salads, desserts, breads, beverages and specialties.
- Adding your own favorite recipes and to share on social media.
- Searching recipes by keyword to quickly locate your favorites.
- "Extras & Help" with essential tools: weight, measure, temperature, fresh to dried herb conversions, high altitude cooking tips, food prep, purchasing, storage and safety.
- Recipes for marinades, pizza, hors d'oerves, breakfast meals, fondues, dips and more.
- Easy onscreen access to "related Recipes" for that special sauce or bouquet garni.
- Frequently asked questions, create shopping lists and Email support.
"And the best part, its just $2.99." said Fenton. "There's no additional subscriptions or charges and no in-app purchases; just a great interactive cooking app, plain and simple."
For more information go to the ChefMate website or the app store.


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For a simple tooth decay or a tooth fracture, there is no need to go so complicated because this can easily be resolved by composite fillings. Naperville patients who come into the dental office with dental caries receive a tooth filling from the best cosmetic dentist. Naperville dentists will begin ... Read More
Tag: composite fillings
September 28, 2016Smiles of Naperville
If one or more of your teeth has significant decay, your Smiles of Naperville dentist will advise you to have a cavity filled to preserve it. In the past, silver amalgam was the only option for filling material. However, most dental patients today prefer composite fillings instead. This is partly ... Read More
March 21, 2016Smiles of Naperville
In decades past, the only choice people had when they needed a cavity filled was for the dentist to use silver amalgam filling material. As a dental patient today, you are fortunate to have the option to receive a composite filling instead. That is because many people expressed concerns about ... Read More
January 7, 2016Smiles of Naperville
Our teeth allow us to do different things in the different activities that we do every day. Mostly, it helps us to chew our food, helps us to speak and maintain our facial structures. Keeping our mouth and teeth healthy can prevent disease, tooth infection and aesthetic dysfunction from occurring ... Read More
May 22, 2015devops
Tooth decay is the most common cause of tooth loss among the young and old population. It usually happens because of poor oral hygiene, wrong food choices and unhealthy lifestyle. Often times, we hear and read the importance of a good oral hygiene to prevent plaque build-up that can cause tooth ... Read More
November 16, 2014devops


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My late 20th century middlebrow center of gravity
Sometimes I like to scroll through this blog for the fun of it, as if I'm walking down Memory Lane to revisit writings about Memory Lane! In addition to enjoying periodic nostalgic memories, I'm reminded of where my own cultural center of gravity is located. I am, at heart, a middlebrow kind of guy, grounded in the late 20th century. Here are 25 reasons why, many of which are drawn from previous posts:
- My MP3 music lists include the likes of 80s and 70s pop hits, old standards featuring music of the Gershwins and Cole Porter, and soundtracks & cast recordings of classic musicals by Rodgers & Hammerstein.
- I still have much of No. 1 on CDs.
- I like Stouffer's French Bread pizza.
- I belong to the Book-of-the-Month Club and occasionally hunt down past BOMC premium books on e-Bay.
- I make my coffee using a drip coffee maker and pre-ground beans.
- Despite my dovish leanings, I enjoy watching old World War Two movies.
- I will indulge myself with an occasional Big Mac.
- I own, and sometimes even read into, a pre-owned set of the Harvard Classics.
- Give me the voices of Olivia Newton-John and Karen Carpenter over those of most of today's female pop singers any day.
- I miss American Heritage magazine.
- I love watching re-runs of The Dick Van Dyke Show on Netflix.
- I still regard Baskin-Robbins ice cream as a treat.
- My leisure reading tastes go to mysteries and suspense, sports books (baseball, football, basketball), and popular history, as well as self-help and psychology.
- Walter Cronkite remains for me the iconic example of a television newscaster with utmost integrity.
- Given a choice, I'll take a casual meal at a favorite diner over a fancy meal with multiple forks.
- I've been a steady subscriber to Sports Illustrated for decades.
- My first computer was a Commodore 64, and I got years of use and fun out of it!
- I continue to rely on Rick Steves for travel advice when planning blessed trips to Europe.
- Pumpkin pie is my favorite Thanksgiving dessert.
- Having my own personal library is deeply meaningful to me.
- Frank Sinatra's "New York, New York," Tony Bennett's "I Left My Heart in San Francisco," and Louis Armstrong's "What a Wonderful World" are simply awesome to me.
- I miss talk radio from the days before it got so politically strident and polarized.
- I regard Stephen King as one of our great contemporary storytellers.
- Growing up, I pursued hobbies such as stamp and coin collecting, science, and playing sports simulation board games — and I still do when time permits!
- There's something thrilling and adventurous about being in a large old train station.
When the TV goes dark (Somehow, I've managed)
For the past ten days, I've been without a functioning television set. This is not exactly the stuff of deprivation or sacrifice, but it is a tad inconvenient, especially now that the pro and college football seasons are in full swing. Fortunately, help is on the way. The other day, I went to an electronics store and ordered a new TV — a fairly basic and surprisingly affordable widescreen model — which should be delivered and set up by the end of the week.
The TV I ordered represents my first purchase of a brand new model since buying a $99 cheapo set some 20 years ago! Every other TV has been a discard or a used one, including the 15-year-old kaput model that will be carted away soon.
Many moons ago, I didn't even have a TV. During law school, I went without. I listened to the radio a lot, and I really enjoyed talk radio programs at a time when that genre was more conversational and fun, rather than a stream of political soapboxes. On occasion I'd go to the TV room in the law school dorm to watch a favorite program or two, or maybe one of my friends would have us over to watch something, but that was about it.
Right after law school, I was working as a Legal Aid lawyer and was barely scraping by, so I didn't have a TV until some benevolent friends gave me an old portable black-and-white set that had been gathering dust. It may seem hard to imagine that I was quite happy with the social and entertainment options in my life at the time without cable or a VCR (these were the pre-DVD and streaming days, folks), but it really was so.
You know what I've rediscovered during this brief time? Listening to football games on the radio can be fun, at least when your team wins. On Saturday I was pulling for Navy to beat Temple and for Notre Dame to beat Michigan. Good results there. But my beloved Chicago Bears took it on the chin against Buffalo, and the Patriots fell apart against Miami.
The weekend reminded me of when I was a kid, listening to games on the radio during a time when lots fewer games were televised.
Of course, this time around I also have the Internet, which I periodically accessed on Sunday to follow the fortunes of my two fantasy football teams. (Two wins there, baby!)
So…radio and the Internet. Old meets new.
Practically speaking, listening to games on the radio made it easier to get some work and chores done. I'm not glued to my TV set when it's working, but even so I realize how easy it is to dump hours into gazing at the screen. I'll keep that in mind once my new TV arrives and everything is up and running.


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Dodge Lady Gaga. Collect Golden Globes. Find the black Oscar nominee.
Winning an Academy Award, it proves, can be nearly an impossible task, at least according to the lighthearted Web game "Leo's Red Carpet Rampage." The game puts players in control of a mini, vintage-style Leonardo DiCaprio in a quest for an Oscar.
And while the game is pure goofiness when it starts — simply mash a couple of buttons to run the red carpet and dodge photographers — it goes dark, and quickly. Players soon will be frantically hitting the keyboard in an effort to "act harder," or muster their way through a quaalude overdose — a nod, no doubt, to DiCaprio's film "The Wolf of Wall Street."
Then, of course, there's this scene:
Games rarely delve into of-the-moment political satire, and the tone throughout "Leo's Red Carpet Rampage" is just nutty enough that when the game nods to the #OscarsSoWhite movement, it isn't heavy-handed. In fact, "Leo's Red Carpet Rampage" succeeds at mocking the lack of diversity among the Academy Awards nominees while playing up the ridiculousness of award campaigns in general.
Though it probably isn't more than a nice diversion, it does succeed in parodying the absurdity of the Oscar race, which dominates entertainment media for a number of months leading up to the Academy Awards on Feb. 28.
Follow me @toddmartens
What might Oscar learn from Grammy?
#OscarsSoWhite? Maybe not in 2017. Here's an early look at promising contenders from upcoming films
David Bowie, a video game pioneer as well
Diversity takes the win at 2016 Grammys with moments that crossed race, age and gender


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Leverage Browser Cache and Minimize DNS requests with OMGF (Optimize My Google Fonts, formerly known as CAOS for Webfonts). On the other hand, millions of people delve into the intricacies of graphic editor solely for himself: for example, for high-quality retouching of images and overlay them complex effects. A typeface ...Read More »
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TERMIUM Plus®
The Government of Canada's terminology and linguistic data bank.
HYDROTHERMAL SYNTHESIS [1 record]
Record 1 - internal organization data 2001-08-14
Record 1, English
Record 1, Subject field(s)
- Mineralogy
- Geochemistry
- Thermal Springs - Uses
Record 1, Main entry term, English
- hydrothermal synthesis
1, record 1, English, hydrothermal%20synthesis
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
Mineral synthesis in the presence of water at elevated temperatures. 1, record 1, English, - hydrothermal%20synthesis
Record 1, French
Record 1, Domaine(s)
- Minéralogie
- Géochimie
- Thermalisme
Record 1, Main entry term, French
- genèse cristalline hydrothermale
1, record 1, French, gen%C3%A8se%20cristalline%20hydrothermale
feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record 1, Spanish
Record 1, Textual support, Spanish
Copyright notice for the TERMIUM Plus® data bank
© Public Works and Government Services Canada, 2020
TERMIUM Plus®, the Government of Canada's terminology and linguistic data bank
A product of the Translation Bureau
Language Portal of Canada
Access a collection of Canadian resources on all aspects of English and French, including quizzes.
Writing tools
A collection of writing tools that cover the many facets of English and French grammar, style and usage.
Glossaries and vocabularies
Access Translation Bureau glossaries and vocabularies.
- Date Modified:


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★★★★★ | Mango vinaigrette salad
★★★★★ | Mango vinaigrette salad
★★★★★ | Mango vinaigrette salad
By Let's Cooking
^^ CLICK TO SEE FULL RECIPES ^^ This formula contains a few fixings plentiful in nutrient C and different phytochemicals that add to the correct working of the body. It is a perfect formula for a solid weight reduction. | #let'scooking
Planning Time: 15 mins
Coz Time:
Total time: 15 mins
- 120g of cucumber
- 120g of tomatoes
- 1 medium build up free sleeve
- 1 pepper of your decision (woman finger, I utilized jalapeño in light of the fact that it is zesty)
- A bunch of coarsely hacked cilantro or parsley
- Salt to taste (discretionary)
- Tahiti or Sicilian Lemon Juice
- 2 tablespoons unfiltered or sanitized apple juice vinegar (Bragg use, yet utilize whatever is accessible to you)
- ½ teaspoon additional virgin olive oil (discretionary, yet included to help ingest fat-solvent nutrients)
- First clean and shakers the cucumber, at that point expel the seeds from the tomatoes and bones.
- Shakers the mango and spot in a bowl alongside the cucumber and tomatoes.
- Slash the pepper that can be fill in for bean stew or chives and include the bowl.
- Include the cilantro or slashed parsley.
- Crush the lemon.
- Season with salt to taste.
- Furthermore, include the apple juice vinegar.
- Blend many well to join the fixings.
- And afterward serve or refrigerate.
- Preservation is one day in the fridge.
Rated 5/5 based on 70.000 customer reviews


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JACKSON – Mississippi is expected to hire its first medical examiner in 15 years by Nov. 1, and Department of Public Safety Commissioner Steve Simpson said he's hopeful at least two other associate pathologists will soon be on board to help handle the state's autopsy cases.
"We have one doctor who has an engagement letter to come here Nov. 1. I'm interviewing another doctor this week in Houston (Texas), and a third has expressed an interest in coming here," Simpson said Tuesday.
Mississippi's contract with a Tennessee-based company that had been performing autopsies for the state ends Friday. Simpson said Global Forensics exercised an option to get out of the contract.
"We've not had any problems with the quality of their work at all. The doctors commute from Tennessee to Mississippi. It has given the coroners some difficulty in having the line of communication they're used to," Simpson said.
It's been nearly two years since the state terminated its contract with Dr. Steven Hayne, a pathologist who came under fire for his work in several criminal cases, including ones that resulted in the exoneration of two men who had been convicted of capital murder.
Mississippi last had a medical examiner in 1995. The void had been filled by Hayne, who handled the majority of autopsies for the state's criminal investigations.
… we'd like to ask for your support. More people are reading the Mississippi Business Journal than ever before, but advertising revenues for all conventional media are falling fast. Unlike many, we do not use a pay wall, because we want to continue providing Mississippi's most comprehensive business news each and every day. But that takes time, money and hard work. We do it because it is important to us … and equally important to you, if you value the flow of trustworthy news and information which have always kept America strong and free for more than 200 years.
If those who read our content will help fund it, we can continue to bring you the very best in news and information. Please consider joining us as a valued member, or if you prefer, make a one-time contribution.Click for more info


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B.A.C.A. News
B.A.C.A. of California
California State Information
|B.A.C.A. California
P.O. Box 402147
Hesperia, CA 92340
|State Executive Board
|State Appointed Positions
|Phone: (408) 930-2131
|Public Relations: (email) Boxer
|State President: (email) Tiny
|Child Liaison: (email) Teacher
|State Vice President: (email) Hoss
|Biker Liaison: (email) Gator
|State Sergeant At Arms: (email) Gimli
|Merchandiser: (email) Noni
|State Secretary: (email) Froggy
|Event Coordinator: (email) Hoss
|State Treasurer: (email) LuLu
|CDO: (email) Mello
|Agency Liaison: (email) Fester
|IT Admin: (email) Merc


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Examination of the glossopharyngeal nerve involves the gag reflex. The examiner may use either a tongue blade or an applicator stick. By touching the posterior third of the tongue, the soft palate, or the posterior pharyngeal wall, the examiner should elicit a gag reflex. The sensory portion of the loop is through the glossopharyngeal nerve;the motor portion is mediated through the vagus nerve.
Another way to test the nerve is to ask the patient to open the mouth widely and to say, ''Ah ... ah.'' Symmetric elevation of the soft palate demonstrates normal function of cranial nerves IX and X. The uvula should remain in the midline.
Taste sensation of the posterior third of the tongue is not routinely tested.
Corticobulbar fibers
Facial nerve nucleus in pons
Facial nerve to upper face
Facial nerve to lower face
Corticobulbar fibers
Facial nerve nucleus in pons
Facial nerve to upper face
Figure 21-16 Innervations of the facial nerve and types of facial weakness. Lesion A produces an upper motor nerve palsy that causes contralateral weakness of the lower face but spares the contralateral forehead. Lesion B produces a lower motor nerve palsy that causes total paralysis of the ipsilateral face.
Was this article helpful?
Have you recently experienced hearing loss? Most probably you need hearing aids, but don't know much about them. To learn everything you need to know about hearing aids, read the eBook, Hearing Aids Inside Out. The book comprises 113 pages of excellent content utterly free of technical jargon, written in simple language, and in a flowing style that can easily be read and understood by all.


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Finn + Emma Organic Cotton Romper - Origami
Finn and Emma
$32.00 $13.00
(You save $19.00)
- Availability:
- Normally ships within 48 hrs
Clearance items are final sale and non-refundable.
When it's time to play hard, it's time for our romper. Made from buttery-soft organic cotton, it features easy-snap fasteners with our signature coconut inserts arranged to make it easy to get to your little one even when they'd rather do anything but get changed. A wardrobe staple for kiddies on the go!
- Color: Origami
- 100% G.O.T.S. certified organic cotton
- Lead and nickel free snaps with wood insert
- Low-impact inks and dyes, great for sensitive baby skin
- Made in India


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March 29th Maker House Workshop Postponed. New date is may 26th
Regular price
Our starter kit contains materials to make 8 Tealight Soy Candles
Pick a blend or create your own:
EMPOWER~ Cinnamon, Tangerine, Rosewood, Ylang Ylang, Wild Orange
BLISS~ Spearmint, Lavender, Lemongrass
LOVE~ Peppermint, Lavender, Geranium
LOLLIPOP~ Key Lime Tangerine Orange Frankincense
ZEN REST~ Patchouli, Lavender, Cedarwood, Orange
HAPPY~ Wild Orange, Peppermint, Litsea, Bergamot, Cassia
DIY kit includes all the goods you will need for next Candle Making event! Contact me with questions, I can customize to meet any group size!
All you need to get started is a stove, pot, glass measuring cup and thermometer!
Add your essential oil choices in Note section at check out. Contact us today and we will help you create your custom order! We have a large selection of Essential Oils, Vintage Teacups, Bowls and Glass to add on your your order!
FREE Delivery Kanata & Stittsville.
FREE Pick Up by appointment.


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TYPE A TP-024 Colour Blind Glasses
CLICK HERE TO ORDER FROM AMAZON TP-024 are our newest addition to the collection offering titanium coating to protect against glare and offer sophisticated UV protection, meaning your eyes will be perfectly protected from the harmful rays of the sun. These glasses will effortlessly enhance your vision as they effectively correct the natural various shades of reds and greens around you. Plus, with a premium protective case and cleaning cloth included, you'll be able to keep your glasses safe and lenses smudge-free.
- (Type A) for mild, moderate or strong deutan, also mild and medium protan colour blindness, correcting for reds, greens, browns, oranges, blues and purples
- For the perfect gift of colour select type A that achieves great results for the vast majority of colour blind people or type B to give that extra boost for more severe colour blindness.
- Advanced light-filtering technology, titanium coated to protect against glare and fully UV protected against the harmful rays of the sun
- Suitable for outdoor use / also indoors in a well-lit environment, watching TV or surfing the internet
Approximately 12% of men and 1% of women have some degree of colour blindness. The most common type of colour blindness is red-green deficiency whereby red-green retinal cone cells have an overlapping response to light. Because their response to light is so similar this causes a wavelength deficiency and therefore the brain is unable to determine red and green efficiently. To compensate for the overlap, PILESTONE has taken existing technology and worked with leading experts to develop new and much improved materials to remove certain wavelengths that enable people with red-green colour blindness greater colour vision.
Pilestone's Returns Policy
Pilestone wants you to be completely satisfied with your purchase. If, for any reason you want to return your glasses then you have a 60-DAY simple money-back guarantee for any glasses purchased through www.pilestone.ca or Amazon.ca
How to return your glasses
Please email us with a brief explanation as to why you wish to return the glasses and we will get back to you with instructions and a returns address.
Please Note:
Once the package is received it will be processed and within 24 hours we will initiate a refund and you will be credited into your bank within 2-5 days.
Returns Policy Details
Please return the glasses in their original condition including all contents as they arrived. Wearing the glasses for weeks and returning in a poor condition is unacceptable.
60-DAY returns from the date we shipped your order.
Please include your order number and/or invoice in the package.
Customers pay return shipping and please ensure the return is Tracked/Signed for and keep your proof of return delivery in a safe place.
Any questions, we're here to help!
Email: email@example.com