text:
Nanocrystals in ligand boxes exhibiting enhanced chemical, photochemical, and thermal stability, and methods of making the same
Dendron ligands or other branched ligands with cross-linkable groups were coordinated to colloidal inorganic nanoparticles, including nanocrystals, and substantially globally cross-linked through different strategies, such as ring-closing metathesis (RCM), dendrimer-bridging methods, and the like. This global cross-linking reaction sealed each nanocrystal within a dendron box to yield box-nanocrystals which showed dramatically enhanced stability against chemical, photochemical and thermal treatments in comparison to the non-cross-linked dendron-nanocrystals. Empty dendron boxes possessing a very narrow size distribution were formed by the dissolution of the inorganic nanocrystals contained therein upon acid or other etching treatments.
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This application claims priority to U.S. patent application Ser. No. 60/417,796, filed Oct. 3, 2002, which is incorporated herein in its entirety.TECHNICAL FIELD OF THE INVENTION
The present invention is related to methods of using organic ligands to stabilize nanocrystals, as well as nanocrystals stabilized by these ligands. More specifically, the present invention provides new compositions and methods involving ligands to enhance the chemical, photochemical and thermal stability of colloidal nanocrystals.BACKGROUND OF THE INVENTION
Colloidal nanoparticles are nanometer-sized solid particles which are of interest for advanced materials applications. Generally, colloidal nanoparticles include colloidal nanocrystals as well as amorphous particles, and can comprise either inorganic or organic solids. For simplicity, inorganic nanocrystals are discussed as the representatives of nanometer-sized solids. The term "nanometer-sized" is typically used to refer to particles with an approximate size range between about 1 nm to about 1000 nm in diameter (one nanometer is 10−9 meter). More typically, "nanometer-sized" refers to an approximate size range between about 1 nm to about 100 nm in diameter.
Usually, nanocrystals are thermodynamically metastable and typically need kinetic stabilization with a monolayer of organic ligands, typically referred to as capping groups, surfactants, stabilizers, and the like. These organic ligands function by having at least one binding site with which to bind the surface atoms of the nanocrystals, while the remaining portion of the ligand molecules provides steric isolation between nanocrystals.
Organic dendrons are regularly branched organic molecules starting from a focal point or molecular branching point, which can be used as ligands for nanocrystals. For simplicity, the term dendron is used to refer to any organic ligand that is substantially branched. With organic dendrons, the molecular focal point typically constitutes the site at which the ligand binding occurs while the periphery constitutes the organic groups that provide steric protection. The number of branching points from the focal point to the terminal groups of the dendron is used to characterize these ligands and is referred to as the number of generations of the dendrons. For instance, generation 3 (G3) dendrons refers to dendrons with three branching points from the focal point to the terminal groups of the dendrons.
Colloidal nanocrystals have generated great fundamental interest in recent years and continue to exhibit tremendous promise for developing advanced materials for a variety of important technical applications. The size-dependent emission is probably the most attractive property of semiconductor nanocrystals. For example, differently sized CdSe nanocrystals can be prepared that emit from blue to red with very pure color. These nanocrystal-based emitters can be used for many purposes, such as light-emitting diodes, lasers, biomedical tags, and the like. Further, magnetic nanocrystals can be used as enhancing agents for magnetic resonance imaging (MRI) in the medical diagnostics industry.
Current research and development activities in colloidal nanocrystals remain hindered by the paucity of reliable processing chemistry. Recent developments in the synthesis of semiconductor, metal, and oxide nanocrystal systems, have placed the need for better processing chemistry in an even more urgent position. The processibility of colloidal nanocrystals is related to the stability of the nanocrystal-ligand complex, which it turn is related to the nature and structure of the surface ligand monolayer, the interface between the inorganic core and the organic ligand monolayer, and the structure of the surface of the inorganic nanocrystals themselves. Yet, knowledge regarding the structure of inorganic nanocrystal surface, the nature of the nanocrystal-ligand interface, and the dynamics and structure of the ligands within the monolayer is limited.
An example of the importance in obtaining stable nanocrystal-ligand complexes is seen in the lifetime of the light emitting diodes (LEDs) based on semiconductor nanocrystals. Presently, this attainable lifetime is relatively short, which is likely the result of the thermal dissociation of organic ligands from the nanocrystal surface during operation of the LED device. Further, the troublesome conjugation chemistry related to promising biological labeling applications using semiconductor nanocrystals has been also found to be associated with the detachment of the organic ligands from the nanocrystal surface. Additionally, the long-pursued enhancement effect of magnetic nanocrystals for magnetic resonance imaging is still in its infancy because of the instability of the ligands on the surface of the nanocrystals.
Two types of stability/instability issues related to nanocrystal/ligand complexes have been identified. Type I instability arises when the organic ligands dissociate from the inorganic core, and the nanocrystal/ligand complex is thus destroyed. In solution, this type instability results in uncontrollable chemical properties of the outer surface of the ligand monolayer and the detachment of the desired chemical/biochemical functions from the inorganic core. In many cases, this Type I instability also induces precipitation of the nanocrystals. In both solid state and in solution, dissociation of the organic ligands and the inorganic core often causes undesired variations of the properties of nanocrystals, such as decrease of either photoluminescence or electroluminescence brightness. Type II instability is associated with the inorganic core of the nanoparticle or nanocrystal being subject to oxidation, etching, and even complete dissolution. Thus, Type II instability normally defeats the function of the original nanocrystal/ligand complex.
Therefore, what is needed are new processing methods to impart and maintain greater stability to the nanocrystal/ligand complex. Both Type I instability resulting from dissociation of organic ligands from the inorganic core, and Type II instability arising from the chemical oxidation, etching, or dissolution of the inorganic core following ligand dissociation should be addressed. Nanocrystal stability is closely associated with the nature and structure of the surface ligand monolayer, the interface between the inorganic core and the organic ligand monolayer, and the structure of the surface of the inorganic nanocrystals themselves, therefore all these aspects of nanocrystal stability should be addressed. If possible, these new processing methods would be applicable to enhance the thermal stability, the redox stability, and the photolytic stability of the nanocrystal/ligand complex.SUMMARY OF THE INVENTION
The present invention addresses the current limitations in nanocrystal stability by affording stable nanocrystal-ligand complexes though improved stability of both inorganic nanocrystal core as well as the organic ligand monolayer. New methods for enhancing stability and new compositions of nanocrystals are provided herein.
Typically, semiconductor nanocrystals were employed because of their general interest, easy detection and relatively better established synthetic and ligand chemistry, however, the methods described herein are equally applicable to nanocrystals that are not semiconductors. Typically, the stability of the CdSe nanocrystal core was enhanced by the epitaxial growth of a thin layer of CdS on their surface. Such core-shell structures can improve the photochemical stability of CdSe nanocrystals by the confinement of the photo-generated charges inside the core material. Additionally, the enhanced stability of the ligand monolayer was achieved by cross-linking substantially all the surface ligands of each nanocrystal.
As will be understood from the description of this invention, the cross-linking of surface ligands of a nanocrystal results in enhanced stability of the ligand sheath surrounding the nanocrystal, as well as the nanocrystal-ligand complex as a whole. Accordingly, one aspect of this invention is improving and enhancing the cross-linking of these ligands to achieve more stable nanocrystal-ligand complexes. To achieve substantial cross-linking, it is not necessary that all of the cross-linkable sites of each ligand engage in a cross-linking reaction. Typically, substantial cross-linking implies that most of the ligands of the nanocrystal-ligand complex engage in cross-linking reactions that serve to enhance and improve stability.
One aspect of this invention is a composition referred to as a "box-nanocrystal," comprising a nanocrystal core usually in the size range between 1 and 100 nm and a plurality of dendron ligands coordinated to the nanocrystal core that are substantially cross-linked. The nanocrystal core of the box-nanocrystal can comprise a metal, an elemental non-metal, an organic compound, a metal oxide, a metal chalcogenide, a metal pnictogen, a metal halide, an alloy, a mixed metal oxide, or a combination thereof. Further, the nanocrystal core of the box-nanocrystal may comprise a boride, a carbide, a silicide, a nitride, a phosphide, an arsenide, an oxide, a sulfide, a selenide, a telluride, a fluoride, a chloride, a bromide, an iodide, or a combination thereof. When the nanocrystal core is an oxide material, then the nanocrystal core may comprises an oxide of silicon, aluminum, titanium, zirconium, iron, nickel, cobalt, manganese, ruthenium, antimony, tin, cerium, barium, vanadium, chromium, lead, copper, indium, yttrium, zinc, mixed oxides thereof, or combinations thereof.
Another aspect of this invention is a similar composition referred to as a "box-nanoparticle," which is a generic term for a composition comprising a crystalline or a non-crystalline nanoparticle core, usually in the size range between 1 and 100 nm, and a plurality of dendron ligands coordinated to the nanoparticle core that are substantially cross-linked. Thus, a box-nanocrystal is a box-nanoparticle having a nanocrystal core. The nanoparticle core of the box-nanoparticle may comprise the same types of materials as those specifically disclosed herein for the box-nanocrystal, including but not limited to, a metal, an elemental non-metal, an organic molecule, a metal oxide, a metal chalcogenide, a metal pnictogen, a metal halide, an alloy, a mixed metal oxide, or a combination thereof. The box-nanocrystals and dendron-nanocrystals disclosed herein are examples of box-nanoparticles and dendron-nanoparticles, therefore the disclosure, reactions, and examples presented herein for box-nanocrystals and dendron-nanocrystals are exemplary for the more general applications to box-nanoparticles and dendron-nanoparticles.
In one aspect of this invention, the nanocrystal core of the box-nanocrystal comprises a CdSe nanocrystal, a CdS nanocrystal, or a CdSe/CdS core/shell nanocrystal. Further, the nanocrystal core can comprise a semiconductor, a magnetic material or compound (including ferromagnetic, ferrimagnetic, paramagnetic, super-paramagnetic, piezoelectric, piezomagnetic, or antimagnetic materials or compounds), a catalyst, an antibial, or materials with other chemical and physical properties. Further examples include, but are not limited to, an organic dye or a bio-active species. The box-nanocrystal and the nanocrystal core of this invention are characterized by an average diameter of typically less than about 100 nanometers.
One feature of this invention is its versatility. Thus, the nanometer-sized solid core of this invention can comprise a range of inorganic or organic compounds and substances, including but not limited to: 1) a metal oxide of varying topologies; 2) a metal-"non-oxide" element composition such as a metal boride, carbide, silicide, nitride, phosphide, arsenide, sulfide, selenide, telluride, halide (fluoride, chloride, bromide, or iodide), and the like; 3) a complex inorganic substance in which more than one metal is combined with an element, for example a bimetallic sulfide or a "mixed oxide" compound such as BaTiO3 or YMnO3; 4) a complex inorganic substance in which a metal is combined with more than one other element, for example, a metal oxycarbide; 5) a metal; 6) other binary or ternary compounds that combine non-metals with non-metals, such as boron nitride, or combine metals and metals, such as a bimetallic alloy; 7) an elemental non-metal such as silicon; 8) an organic compounds such as organic dyes or drug molecules; or 9) combinations or mixtures thereof.
Generally, the box-nanocrystal of this invention comprises cross-linkable dendron ligands that themselves comprise cross-linkable alkene, amine, hydroxyl, ester, halide, ketone, aldehyde, or other common functional groups prior to cross-linking. Typically, the box-nanocrystal of this invention comprises dendron ligands selected from generation 2 (G2), generation 3 (G3), generation 4 (G4), or generation 5 (G5) dendrons, wherein each dendron ligand is functionalized with one, two, three, four, or more binding sites for coordinating to the nanocrystal. In one aspect, the dendron ligands are selected from G3-(hydroxamide)-CH═CH2, G3-(carboxylic)-CH═CH2, G2-(amine)-ester, G3-(thiol)-CH═CH2, G3-(diamine)-OH, G3-(thiol)-OH, G3-(dicarboxylic)-OH/PEG, or G4-(dicaboxylic)-CH═CH2/PEG, prior to cross-linking. When the cross-linkable dendron ligands comprise alkene groups, these alkene groups may be substantially cross-linked by a ring-closure metathesis reaction.
Another aspect of this invention is a nanometer-sized particle, comprising a nanometer-sized solid core usually in the size range of about 1 nm and about 100 nm and a plurality of cross-linkable dendron ligands coordinated to the nanometer-sized inorganic core. In one aspect, the nanometer-sized solid core comprises a nanocrystal.
Another aspect of this invention is the dendron box itself, comprising a plurality of dendron ligands that have been substantially cross-linked, wherein the dendron box has an average inner diameter between about 1 nm and about 100 nanometers, and wherein the nanometer-sized inorganic core has been removed. Thus, this invention further encompasses a method of forming a dendron box comprising contacting a nanocrystal with a plurality of cross-linkable dendron ligands to form a dendron-coated nanocrystal (also called a "dendron nanocrystal"), cross-linking the dendron ligands to form a box-nanocrystal, and removing the nanocrystal from the box-nanocrystal to form the dendron box. Useful cross-linkable dendron ligands include, but are not limited to, G3-(hydroxamide)-CH═CH2, G3-(carboxylic)-CH═CH2, G2-(amine)-ester, G3-(thiol)-CH═CH2, G3-(diamine)-OH, G3-(thiol)-OH, G3-(dicarboxylic)-OH/PEG, and G4-(dicaboxylic)-CH═CH2/PEG. This invention also encompasses the removal of the nanocrystal from the box-nanocrystal by oxidation, acid treatment, thermolysis, and other chemical and photochemical etching techniques.
Another aspect of the present invention is a method of stabilizing a nanocrystal comprising contacting the nanocrystal with a plurality of cross-linkable dendron ligands to form a dendron-nanocrystal, and cross-linking the dendron ligands to form a box nanocrystal. Typically, the nanocrystal is a semiconductor nanocrystal, or is selected from CdS, CdSe, or CdSe/CdS core/shell nanocrystals, but this method of stabilization is also applicable to a number of materials that are not semiconductors. Also typically, the cross-linkable dendron ligands of this method comprise a thiol anchoring group and a plurality of cross-linkable alkene groups. Usually, the cross-linking of the alkene groups is effected by a ring-closing metathesis reaction, to cross-link substantially all the dendron ligands in the dendron-nanocrystal to form the box-nanocrystal.
Thus, in one aspect of this invention, in order to reach the desired global cross-linking, a "Generation 3" (G3) dendron with thiol anchoring group, and eight carbon-carbon double bonds as terminal groups was employed as the surface ligand. The relatively enhanced chemical stability of the dendron ligand-coated nanocrystals (dendron-nanocrystals) enabled the nanocrystal/ligand complexes to readily survive the cross-linking reaction and related purification procedures. Further, the multiple double bonds of each dendron ligand made it possible to obtain global cross-linking of substantially all the dendron ligands on the surface of each nanocrystal to form a "dendron box" around each nanocrystal, termed a "box-nanocrystal". Various methods were also developed that allowed the removal of the nanocrystal from the dendron-box, in which the cross-linked ligands remained intact and provided a useful capsule for a variety of molecular scale host-guest applications.
In one aspect, the present invention addresses the current limitations in nanocrystal stability by creating stable nanocrystal-ligand complexes by providing a globally cross-linked ligand monolayer for each nanocrystal. Through such cross-linking, the ligands around each nanocrystal form a ligand box. For simplicity, the resulting cross-linked nanocrystal-ligand complex is called a box-nanocrystal. As disclosed herein, this method is very versatile and is applicable to synthesizing and stabilizing a range of inorganic nanocrystals with different compositions, including but not limited to, metals, oxides, and chalcogenides.
Organic dendron ligands with functionalized terminal groups, especially terminal groups that can be cross-linked (referred to as cross-linkable dendrons), are particularly suited for the global cross-linking process if a large number of the terminal groups of each dendron are available for cross-linking. This feature affords a high probability that a dendron ligand will be cross-linked with its neighboring ligands in the ligand monomer of a given nanocrystal, thereby providing the desired stability to the box-nanocrystal. For example, a G3 dendron can have at least 8 terminal functional groups, although a global cross-linking of all ligands around each nanocrystal likely requires only two to three cross-linking sites.
While not intending to be bound by this statement, because dendrons are molecules with defined structures, all cross-linking sites could be readily designed at a close proximity to each other, which was thought to make the cross-linking reactions occur in a high yield. Further, the dendron ligands of this invention are typically characterized by flexible dendron chains, which is thought to allow significant intermolecular (that is, interdendron) chain tangling, a feature which should further assist intermolecular cross-linking. To enhance the bonding between nanocrystals and the dendron ligands, bidentate and multidentate binding sites may be designed at the focal point of the dendron ligands.
Different types of cross-linking strategies are suited for the global cross-linking reactions of all dendron ligands around each nanocrystal of this invention. Typically, maximum box-nanocrystal stability is achieved when a cross-linking reaction affords a substantially global cross-linking reaction. The formation of the dendron box around each nanocrystal to form a box-nanocrystal was confirmed by NMR, IR, TEM, and mass spectroscopy. The superior chemical, photochemical and thermal stability of the resulting box-nanocrystals were quantitatively or semi-quantitatively examined.
The resulting stable nanocrystals should be immediately applicable to many technical applications of nanocrystals, including but not limited to, LEDs, solid state lasers, biological labeling, MRI, drug delivery using magnetic nanocrystals, and the like. Further, cross-linked nanocrystal-ligand complexes (box-nanocrystals) are also of interest for many fundamental studies, such as measurement of the size-dependent melting point of nanocrystals and studies on the size-specific reactivity of nanocrystals.
In addition, empty dendron boxes without the enclosed nanocrystal, formed by the dissolution of inorganic nanocrystals in an etching solution, are also provided by the present invention. These empty dendron boxes represent a new class of nanometer sized polymer capsules, which are nearly monodisperse, soluble, stable, and which possess a very thin periphery. These empty dendron boxes find utility in may ways, including as molecular sieves, in host-guest applications, as encapsulating agents for drug delivery, molecular imprinting, and the like.
Accordingly, it is one aspect of the present invention to provide new cross-linked nanocrystal-ligand structures (box-nanocrystals) to impart and maintain greater stability to the nanocrystal-ligand complex.
It is another aspect of this invention to develop new processing methods that will prevent dissociation of organic ligands from the inorganic nanocrystal core, and avoid the thermal instability, chemical oxidation, etching, photochemical instability, or dissolution of the inorganic core itself.
Another aspect of this invention is the enhancement of the stability of the ligand monolayer by substantially cross-linking all the surface ligands of each nanocrystal through methods that include, but are not limited to, ring-closing metathesis (or RCM) reactions, to form cross-linked nanocrystal-ligand structures.
It is another aspect of this invention to develop and utilize a dendrimer-bridging strategy for the formation of box-nanocrystals.
Another aspect of this invention is the use of the improved stability of dendron-coated nanocrystals (referred to as dendron-nanocrystals) to provided the needed stability to the nanocrystal-ligand complex, such that it can remain intact during the cross-linking reaction of the dendron ligands and related purification procedures.
Still another aspect of this invention is the global cross-linking of substantially all the dendron ligands on the surface of each nanocrystal to form a dendron box around each nanocrystal, that is, to provide a box-nanocrystal.
Yet a further aspect of this invention is the formation of an empty dendron box, without the enclosed nanocrystal, which can be used as nanometer-sized polymer capsules, new types of molecular sieves, in host-guest applications, and the like.
Another aspect of this invention is the conjugation of chemical and biochemical functional species, including but not limited to DNA, RNA, peptides, polypeptides, nucleic acids, amino acids, sugars, proteins, antibodies, and the like, onto the surface of box-nanocrystals.
Yet another aspect of this invention is the detection of bio-active species using the stable box-nanocrystals, wherein binding of the bio-active species to the biomedical functional species attached onto the surface of box-nanocrsytals occurs specifically. Another aspect of this invention is the detection of avidin by this method.
These and other features, aspects, objects, and advantages of the present invention will become apparent after a review of the following detailed description of the disclosed embodiments and appended claims, in conjunction with the drawings described as follows.
The present invention provides a class of highly stable nanocrystal-ligand complexes, referred to as box-nanocrystals, and methods and processes for kinetically stabilizing nanocrystal complexes. The enhanced stability of the ligand monolayer was achieved by the substantially global cross-linking of all the dendron ligands on the surface of each nanocrystal to form a dendron box around each nanocrystal. The cross-linking reactions may be achieved by a variety of methods including, but not limited to, ring-closing metathesis reactions, dendrimer-bridging, and complex formation. The present invention further provides for the formation of an empty dendron box without the enclosed nanocrystal, which can be used as nanometer-sized polymer capsules for a variety of host-guest type complexes and applications.Design and Synthesis of Dendron Ligands for Box-Nanocrystals
The formation of box-nanocrystals typically employs dendron or other branched ligands having a certain number of cross-linking points for the global cross-linking reactions involving all ligands around each nanocrystal. The backbone and periphery of the dendrons should be flexible to afford significant intermolecular chain tangling and reduce steric factor for cross-linking reactions to occur.
The focal point of the dendrons, that is an initial molecular branching point, was chosen as the bonding point to the surface of nanocrystals. To enhance the overall bond strength between dendron ligands and the nanocrystal, bidentate and multidentate ligands were also considered. For nanocrystals with different compositions, different bonding or coordination sites on the dendron ligands were typically selected. For example, nanocrystals comprising noble metals or most semiconductor materials typically coordinated with relatively soft ligand bonding sites, such as thiols, amines, phosphines, phosphine oxides, carboxylic acid, phosphonic acids, phosphinic acids, and the like. Nanocrystals comprising transition metals or transition metal compounds typically bonded with ligand sites such as hydroxamate, sophonic, silicate, amines, phosphines, phosphine oxides, carboxylic acid, phosphonic acids, phosphinic acids, and the like. Nanocrystals of some elemental non-metals, such as silicon and germanium, typically bonded with ligands comprising alkene, halide, or hydroxyl groups, and the like. The binding sites for organic nanoparticles varied by the structure and surface of the nanoparticles. Various choices for dendron bonding or coordination sites used for other types of nanocrystal surface atoms or ions can be found in standard references on inorganic coordination chemistry and organic chemistry.
Formation of Box-Nanocrystals via Ring-Closing-Metathesis (RCM) Reactions
The ring-closing metathesis (RCM) reaction comprises one method of cross-linking the dendron ligands surrounding a nanocrystal to form box-nanocrystals. If the dendron ligand comprises terminal groups which are all or partially carbon-carbon double bonds, RCM reactions can readily occur using any ring-closing metathesis catalyst, including but not limited to the Grubbs catalyst.
A typical RCM cross-linking process of this invention employs the second generation Grubbs' ruthenium catalyst as described in detail in Example 11. The catalyst (2 mol % per alkene group) was added to a solution of the dendron-nanocrystals. In calculating this concentration of alkene groups, the amount of ligands on the surface of CdSe/CdS core/shell nanocrystals was estimated by assuming a close packing of ligands on the surface of nanocrystals. The box-nanocrystals that were prepared were precipitated out of solution with an appropriate solvent, and separated by centrifugation and decantation. The reactions provided herein may be altered when the size and composition of the nanocrystals are different.Formation of Box-Nanocrystals via Dendrimer-Bridging Reactions
Another method of cross-linking the dendron ligands surrounding a nanocrystal to form a box-nanocrystal is to globally cross-link these ligands using a second, linker molecule. Thus, the terminal functional groups of dendron ligands are selected such that they can be globally cross-linked using a linker molecule.
Dendrimers are closely related to the dendrons. However, unlike a dendron, a dendrimer does not have a focal point. In general, when two, three or four dendrons chemically linked to each other at their focal points, a dendrimer molecule is formed. Dendrimer molecules with flexible backbones worked extremely well as the linker species. Thus, while functionalized dendron ligands used to coordinate to and surround the nanocrystal core, dendrimer molecules with flexible backbones and functionalized with reaction-complimentary functional groups are used as the linkers by which to globally link the coordinated dendron ligands around the nanocrystal core. While not intending to be bound by this statement, it is believed that the bulky, flexible structure of the dendrimer facilitates this ease of cross-linking. When one functional groups of a multi-functional dendrimer reacts with one terminal group of a dendron ligand, the steric bulk of the dendrimer likely prevents access of another dendrimer molecule to the terminal functional groups on a neighboring dendron ligand. Thus, the functional groups of the other flexible branches on the same dendrimer have a greater chance to react with the terminal groups on the neighboring dendron ligand at the same nanocrystal core. As a result, global cross-linking is possible.
Experimentation also revealed that small bifunctional or trifunctional linker molecules were typically not optimum for the formation of box-nanocrystals in this type of reaction, because these small multifunctional linkers usually have rigid molecular structures. These structural features typically result in the cross-linking reactions competing with mono-coupling events between the terminal functional groups and the small linker species, thereby resulting insufficient cross-linking.Properties of the Box-Nanocrystals and Experimental Evidence for their Formation
Various physical and chemical properties of the box-nanocrystals of this invention were observed in the course of characterizing these species by a variety of methods. Further, this experimental evidence demonstrated that the nanocrystal core remained intact in the box-nanocrystal, and that the cross-linking reaction did not result in degradation or loss of the dendron ligand sheath or casing from the nanocrystal core. For example, the key optical properties of the nanocrystals were verified to be substantially identical before and after the cross-linking process. Thus, the absorption and emission spectra of semiconductor nanocrystals did not change after the cross-linking.
The completion of the cross-linking reactions was determined by NMR and IR spectroscopy by detecting certain fingerprint features of the complex. For example, as shown in
In addition, strong evidence of the "global" nature of the cross-linking of substantially all the dendron ligands of a given nanocrystal was provided by mass spectrometry measurements, using the clear solution obtained by the HCl digestion, as shown in
A measure of the average number of the dendron units on the surface of each box-nanocrystal before HCl digestion was obtained as follows. Nanocrystals after the cross-linking process were carefully purified and the purity was examined by 1H NMR spectroscopy. Typically, if any free small molecules existed in the solution, very sharp and intense 1H NMR signals would be detected. A known amount of the purified nanocrystals in the form of fine powder was weighed to determine the total mass of the nanocrystal-ligand complex, and then, those nanocrystals were dispersed in a known-volume solvent. The total number of inorganic nanocrystals in the solution was determined by the absorbance of the solution and the extinction coefficient of the nanocrystals. The size of the nanocrystal core was determined by the first peak of the absorption spectrum or transmission electron microscopy measurements. With this information in hand, the average number of the dendron units per nanocrystal could be easily calculated, through standard chemical methods.
This agreement between the gravimetric measurements and the mass spectrometry measurements on the number of dendron units before and after the HCl digestion revealed two important features. First, the mass peak of the empty boxes should be the signal from the singly-charged ions. Second, the cross-linking between nanoparticles was insignificant. While not intending to be bound by this statement, this lack of significant inter-particle cross-linking probably arises from an entropic effect, given the closely packed nature of the double bonds at the outer surface of the ligand monolayer on each nanocrystal, as well as the relatively low molar concentration of the nanocrystals.
Transmission electron microscopy (TEM) measurements also provided strong evidence that inter-particle cross-linking was insignificant.
Further, the formation of box-nanocrystals often altered the solubility of the nanocrystals in a variety of solvents in ways consistent with the expected structure resulting from intra-particle cross-linking. For example, carbon-carbon double bond-terminated box-nanocrystals formed by RCM reactions often became only soluble in aromatic solvents or polar organic solvents, such as benzene, DMSO, and DMF. The solubility of the box-nanocrystals was considered to be consistent with the resulting structures of the cross-ring metathesis.Stability of Box-Nanocrystals Under Severe Chemical Conditions
The present invention also provides new compositions and methods involving ligands to enhance the chemical, photochemical and thermal stability of colloidal nanocrystals. Examples of how CdSe box-nanocrystals exhibit superior stability against chemical treatments are seen in both their stability in the presence of strongly acidic solutions and in the presence of strongly oxidizing environment, as illustrated in
A strong oxidant, H2O2 (0.15 mol/L), was found to etch CdSe, as well as many other semiconductor nanocrystals. The dendron-nanocrystals were much less stable than the corresponding box-nanocrystals when they were exposed to H2O2.
Further, the CdSe/CdS core/shell nanocrystals were generally more stable than the corresponding plain core nanocrystals. In particular, the core/shell box-nanocrystals did not show any detectable change upon exposure to H2O2 at a similar concentration for at least several hours. Further, the chemical stability of box-nanocrystals and their precursors, dendron ligands coated nanocrystals (dendron-nanocrystals), is a function of the chemical composition of the inorganic nanocrystals. For instance, box-nanocrystals with a metal oxide nanocrystal core were found to be generally more stable than the metal chalcogenide nanocrystals.Thermal Stability of Box Nanocrystals
Thermal stability of the nanocrystals was examined by sintering the purified nanocrystal powder at 100° C. for a various amounts of time under vacuum. Except for the sintering process, the purification, storage and other processes were all handled in air under ambient conditions. It has been observed that nanocrystals coated with typical commercial ligands such as mercaptoacetic acid often do not survive the purification process, which may include repeated steps of dissolution, precipitation, and air-drying. In contrast, the dendron-nanocrystals of this invention survive these routine treatments without having their solubility substantially effected.
The CdSe dendron-nanocrystals did not survive the harsh heating treatment (100° C., vacuum), although they could be dried under vacuum at room temperature. After sintering, the CdSe dendron-nanocrystals became insoluble in typical solvents. By comparison, the solubility of the CdSe box-nanocrystals was well-maintained after this sintering process, and no change in the optical properties of the nanocrystals was detected. As shown in
The excellent thermal stability of box-nanocrystals suggests that the known, irreversible aggregation of colloidal nanocrystals in solid state can be efficiently halted by "sealing" each nanocrystal inside a dendron box by the process of cross-linking the dendron ligands. It is believed that cross-linking both isolates the nanocrystals from each other and makes it very difficult for a nanocrystal to escape its box. Thus, the poor stability properties of colloidal nanocrystals in general, which cause purification and storage problems, can be improved by sealing those nanocrystals inside the desired dendron boxes. Further, the performance of solid state devices made of semiconductor nanocrystals, such as LEDs and solid state lasers, can be significantly enhanced by the improved stability of box-nanocrystals using semiconductor nanocrystals. In addition, the thin cross-linked dendron monolayer of the box-nanocrystals should also be ideal for efficient charge injection for solid state optoelectronic devices.
Typically, the stability of box-nanocrystals and dendron-nanocrystals against thermal treatments depends on the composition of the inorganic nanoparticle core. For example, under the same conditions described herein for the CdSe nanocrystals (100° C. and vacuum), Fe3O4 dendron-nanocrystals survived a twenty-hour thermal treatment.Photochemical Stability of Box-Nanocrystals
The photochemical stability of box-nanocrystals in air under UV radiation was studied following the established procedure (14,15).
The dendron-nanocrystals of the current ligand system were moderately stable against photo-oxidation. The plain core CdSe dendron-nanocrystals were gradually photo-oxidized and precipitated out of the solution after about 35 hours. In contrast, the CdSe/CdS core/shell dendron-nanocrystals were significantly more stable than the plain core dendron-nanocrystals, and its precipitation occurred after about 100 hours inside a photo-oxidation chamber.
The box-nanocrystals of both the plain core and the core/shell nanocrystals were much more stable than the corresponding non-cross-linked dendron-nanocrystals. For the plain core box-nanocrystals, there was no observable oxidation of the inorganic core detected after the sample was in the photo-oxidation chamber for about 125 hours, although precipitation was observed to be quite rapid. In the case of the core/shell box-nanocrystals, photo-oxidation was not detected for 27 days (650 hours). After 27 days exposure in the photo-oxidation chamber, the precipitation of the nanocrystals occurred in a rapid fashion and was very similar to that of the CdSe box-nanocrystals shown in
The core/shell nanocrystals of this invention are typically prepared in the presence of surface amine ligands. The photoluminescence (PL) of the core/shell nanocrystals partially remained after the replacement of these surface amine ligands by the thiol-based dendrons such as the ones shown in
The PL properties of the core/shell nanocrystals varied in an interesting fashion under chemical oxidation and photo-oxidation conditions, as the PL brightness decreased slightly at the beginning stages of the chemical or photo-chemical oxidation. After this initial stage, the PL brightness typically increased significantly, usually with at least a two-fold increase in the relative intensity of the PL peak. In all experiments performed so far, this increase in PL brightness was always associated with a slight blue shift of the absorption spectrum. A similar PL brightening was also observed if the core/shell dendron-nanocrystals were stored in air with room light after several weeks.
The plain core nanocrystals, including both dendron-nanocrystals and box-nanocrystals, did not emit immediately upon replacing the original surface amine ligands with the thiol based dendron ligands. This was true even when those highly luminescent CdSe nanocrystals isolated at the "bright point" (of highest PL quantum yield) in a growth reaction (see. Qu, et al., Journal of the American Chemical Society, 2002, volume 124, page 2049-2055) were employed for the surface replacement. In addition, no subsequent treatments including cross-linking, thermal sintering, chemical oxidation, and photochemical oxidation, revived a noticeable PL for the plain core nanocrystals.Formation of Empty Dendron-Boxes
One aspect of this invention is the use of ring-closing metathesis (RCM) reactions for cross-linking the branches of the dendron ligands bound to the surface of the nanocrystals described herein. The resulting structures in which a nanoparticle is encapsulated in a dendren box is termed a box-nanocrystal. In some cases, the nanocrystal core of the box-nanocrystal was chemically removed to form an empty dendren box. These dendrien boxes are expected to be useful as molecular imprinting structures and drug delivery carriers.
The size distribution of empty dendron boxes revealed by mass spectroscopy and illustrated in
The inorganic nanocrystals disclosed herein were able to escape the dendron boxes after the chemical bonds between the inorganic nanocrystals and the ligands boxes were destroyed. Such permeability is desirable for drug delivery, molecular imprinting, and a variety of host-guest chemical applications. Such dendron boxes are likely to be the largest empty dendron boxes with a very narrow size distribution currently available. In contrast to the large cavity of the box, the wall of the box based on a G3 dendron itself is quite thin, which is also expected to make these dendron-boxes useful for molecular imprinting and other types of host-guest applications.Conjugation Chemistry of the Box-Nanocrystals
In a further aspect of this invention, the nanocrystals of this invention were chemically coupled to certain chemical or biochemical functional groups, which tailored them for various biochemical or medical applications. This process was relatively simple for box-nanocrystals due to their superior stability and robust chemical and physical behavior. A range of conjugation chemistry of box-nanocrystals is possible, varying according to differences in the structure and properties of the box-nanocrystal's periphery, backbone, bonding, and the inorganic core. For instance, the terminal carbon-carbon double bonds of the box-nanocrystals shown in
The amine box-nanocrystals shown in
The conjugation strategies described herein can readily be used for conjugation of bio-functional species onto box-nanocrystals. For the box-nanocrystals assembled by RCM reactions, bio-conjugation can be readily performed by activating the double bonds that result from the RCM reaction, with bromo groups, one example of which is illustrated in
Using the amine box-nanocrystals shown in
With the incorporation of more biotin units on each nanocrystal surface, the original hydrophilic box-nanocrystal gradually became hydrophobic due to the poor hydrophilic nature of the biotin reagent. Accordingly, the biotinylated box-nanocrystals with high biotin density on the surface precipitated out from the typical reaction solution (DMSO:H2O is 1:1 by volume). Therefore, the amount of biotin on each nanocrystal may be controlled in such a manner that the nanocrystal maintains its solubility and at the same time exhibits maximum number of binding sites for avidin.
The biotinylated core/shell box-nanocrystals exhibited the same stability as amine box-nanocrystals. For example, the PL intensity of biotinylated core/shell box-nanocrystals remained constant for hours under the standard photo-oxidation conditions, and for at least several months under ambient conditions. The excellent stability of bio-conjugated box-nanocrystals provides an essential basis for developing quantitative binding assays using bio-functional box-nanocrystals.Detection of Bio-Active Species Using Box-Nanocrystals
Generally, bio-detection systems using semiconductor nanocrystals have been at a qualitative or non-reproducible level largely because of the instability of the nanocrystals employed. In contrast, this invention provides quantitative and reproducible detection of bio-targets using the box-nanocrystals disclosed herein.
Thus, another aspect of this invention is the bio-medical applications of box-nanocrystals, which were demonstrated using biotin-avidin binding assay with biotinylated box-nanocrystals. Avidin is a glycoprotein which contains four identical subunits, with each subunit possessing one binding site for one biotin molecule. The strong, yet non-covalent, binding between avidin and biotin makes it very useful in bio-conjugation chemistry. The biospecificity of the avidin-biotin interaction is similar to antibody-antigen or receptor-ligand recognition, but is characterzed by a greater affinity constant
Upon the addition of a certain amount of avidin (75 pmol) in PBS buffer to the biotinylated box-nanocrystals in DMSO and H2O solution (1:1/v:v), the clear solution turned to turbid immediately, indicating the formation of avidin-biotin/box-nanocrystal conjugates. This turbid solution was centrifuged for a few minutes to obtain a clear top solution for UV-Vis and PL measurements. The addition of avidin was continued until the solution was almost colorless.
Non-specific binding of the box-nanocrystals to biological targets was also examined. Three sets of experiments were performed to exclude non-specific binding in this system. In the first control experiment, the same volume of PBS buffer without avidin was added into the biotinylated box-nanocrystal solution, and no precipitate was observed. The UV-Vis absorbance and PL intensity after calibration of dilution effect kept as constants with repeated centrifugation and addition of the buffer solution (
The second control experiment was designed to study the non-specific binding of avidin with the amine groups on the surface of the box-nanocrystals. The same amount of avidin was added to the solution of amine box-nanocrystals under exactly the same conditions for the biotinylated box-nanocrystal system. Again, no precipitation was observed using the amine box-nanocrystals, and no change of either UV-Vis absorbance and/or PL intensity was observed during the continuous addition of the avidin solution (
The third experiment was designed to directly verify that the precipitation of the nanocrystals observed in
The present invention is further illustrated by the following examples, which are presented for illustration purposes and which are not to be construed in any way as limiting the scope of the present invention. On the contrary, it is to be clearly understood that resort may be had to various other embodiments, modifications, and equivalents thereof which, after reading the description herein, may suggest themselves to one of ordinary skill in the art without departing from the spirit of the present invention or the scope of the appended claims.
General experimental information and sources of precursor chemicals are as follows. Triphenylmethanol, 2-aminoethanethiol hydrochloride, bromoactyl bromide, dially amine, triethylsilane, trifluoroacetic acid (TFA), tetramethylammonium hydroxide, N,N′-disuccinimidyl carbonate (DSC), 4-cyanobenzaldehyde, 4-cyanobenzoic acid, succinic anhydride, 4-(dimethylamino)pyridine (DMAP), ethylenediamine, anhydrous methyl sulfoxide (DMSO), trioctylphosphine oxide (TOPO, technical grade, 90%), trioctylphosphine (TOP, technical grade, 90%) cadmium acetate hydrate (99.99+%), selenium powder (100 mesh, 95%), anhydrous toluene (99.8%), Celite™, Grubbs' catalyst (second generation) were all purchased from Aldrich (Milwaukee, Wis.). Anhydrous ethyl ether, benzene, chloroform, N,N-dimethylformamide (DMF), acetone, ethyl acetate, methanol, triethylamine and K2CO3, were all purchased from EM Science (Cincinnati, Ohio). Unless otherwise specified, all chemicals and reagents were used as received without further purification. Immunopure avidin, Sulfo-NHS-biotin were purchased from Pirece.EXAMPLE 1 General Synthesis of a Symmetric Generation 3 (G3) Dendron Ligand
A symmetric G3 dendron ligand 6 was synthesized as shown in
Triphenylmethanol (22.9 g, 88 mmol) was added to a solution of 2-aminoethanethiol hydrochloride in trifluroacetic acid (TFA, 40 mL). The resulting solution was stirred at room temperature for about 40 min. The TFA was then removed under reduced pressure and the residue was triturated with diethyl ether. The white precipitate that formed was filtered, partitioned with aqueous NaOH (40 mL, 1N), and extracted with ethyl acetate (3×50 mL). The combined organic layers were dried over Na2SO4. The product was obtained as a white solid after solvent evaporation (23.8 g, yield 85%). ESI-MS(m/z): 320 (M+H+). 1H NMR δD (CDCl3): 7.21˜7.43 (m, 15H, phenyl), 2.58 (t, 2H, SCH2), 2.32 (t, 2H, CH2N).EXAMPLE 3 Synthesis of Bis-(2-amino-ethyl)-(2-tritylsulfanyl)-amine (3)
A CH3CN (135 mL) solution of compound 2 (9.3 g, 29 mmol) and N-(tert-butoxycarbonyl)aziridine (12.3 g, 87 mmol) was refluxed for 3 days. The solvent was then removed under reduced pressure and the residual oil was purified by silica gel column chromatography with CHCl3 as the eluent. This procedure provided 12.3 g of BOC-protected G1 dendron as light yellow solid. This solid was then treated with TFA, dried under reduced pressure, and partitioned with aqueous NaOH to give compound 3 as yellow oil (7.9 g, yield 68%). ESI-MS (m/z): 406 (M+H+). 1H NMR δD (CDCl3): 7.23˜7.44 (m, 15H, phenyl), 2.78 (t, 2H, SCH2), 2.60˜2.70 (m, 4H, CH2NH2), 2.27˜2.35 (m, 6H, CH2N).EXAMPLE 4 Synthesis of N,N-diallyl-2-bromo-acetamide (4)
Bromoacetyl bromide (45 g, 0.22 mol) in ethylacetate (25 mL) was added dropwise to a solution of diallyl amine (9.6 g, 0.1 mol), Et3N (15 mL), in ethyl acetate (50 mL) at 0° C. The reaction was then warmed to ambient temperature, and the stirring was continued for about 3 h. The resulting mixture was washed with saturated NaHCO3 solution (3×100 mL) and dried over Na2SO4. The evaporation of the solvent gave 16.2 g of product as a light yellow oil (yield 75%). ESI-MS (m/z): 218 (M+H+). 1H NMR δD (CDCl3): 5.75 (m, 2H, CH), 5.19 (m, 4H, CH2), 3.95 (d, 4H, NCH2), 3.81 (2, 2H, BrCH2).EXAMPLE 5 Synthesis of N,N-dially-2-({2-[[2-bis-diallylcarbamoylmethyl-amino)-ethyl]-(2-trutylsulfanyl-ethyl)-amino]-ethyl}-daillylcarbamoylmethyl-amino)-acetamide (5)
Compound 4 (0.9 g, 4.1 mmol) was added to a solution of compound 3 (0.4 g, 1 mmol), K2CO3 (0.84 g, 6 mmol, in 3 mL of H2O) in THF (15 mL). The reaction mixture was then heated at 65° C. overnight, after which time the solvent was removed under reduced pressure. The residue was purified by silica gel column chromatography, eluted with CHCl3/MeOH (85:15/v:v) to obtain a light yellow product (0.7 g, yield 73%). ESI-MS (m/z): 954 (M+H+). 1H NMR δD (CDCl3): 7.21˜7.33 (m, 15H, phenyl), 5.68 (m, 8H, CH), 5.07 (m, 16H, CHCH2), 3.44˜3.88 (m, 24H, COCH2, CH2CHCH2), 2.81 (m, 2H, SCH2), 2.31 (m, 10H, NCH2).EXAMPLE 6 Synthesis of N,N-dially-2-({2-[[2-bis-diallylcarbamoylmethyl-amino)-ethyl]-(2-mercapto-ethyl)-amino}-ethyl}-diallylcarbamoylmethyl-amino)-acetamide (6)
Triethylsilane (0.1 mL) was added to a solution of 5 (0.5 g, 0.5 mmol) in trifluroacetic acid (TFA, 10 mL). The white precipitate that formed upon triethylsilane addition was removed by filtration through Celite™. The TFA was then removed under reduced pressure to give the product as a light yellow oil (0.34 g, 95%). ESI-MS (m/z): 712 (M+H+). 1H NMR δD (CDCl3): 5.68 (m, 8H, CH), 5.07 (m, 16H, CHCH2), 3.44˜3.88 (m, 24H, COCH2, CH2CHCH2), 2.75 (m, 2H, SCH2), 2.31 (m, 10H, NCH2).EXAMPLE 7 Synthesis of CdSe and CdSe/CdS Core/Shell Nanocrystals
Trioctylphosphine oxide (TOPO)-capped or amine-capped CdSe nanocrystals were synthesized using the standard "greener" methods reported previously. (See: Aldana, J.; Wang, Y.; Peng, X. J. Am. Chem. Soc. 2001, 123, 8844; Qu, L.; Peng, Z. A.; Peng, X. Nano Lett. 2001, 1, 333; and Qu, L.; Peng, X. J. Am. Chem. Soc. 2002, 124, 2049-2055; which are incorporated by reference in their entireties.) The typical size of the nanocrystals used in this work was about 3.3 nm with the first excitonic absorption peak at 550 nm. The CdSe/CdS core/shell nanocrystals were synthesized through the solution atomic layer epitaxy (SALE) method. The core size of the core/shell nanocrystals was about 3.5 nm and the shell thickness was about 1.5 monolayers of CdS.EXAMPLE 8 Synthesis of an Asymmetric Generation 3 (G3) Dendron Ligand (FIG. 18)
Synthesis of tosylated triethylene glycol monomethyl ether 2. To a solution of triethylene glycol monomethyl ether 1 (32.8 g, 0.2 mol), triethyl amine (20.4 g, 0.2 mol) and pyridine (2.0 g, 0.026 mol) in CH2Cl2 (100 mL), and p-toluene sulfonyl chloride (42 g, 0.22 mol) in CH2Cl2 (100 mL) was added slowly over a period of 2 h in an ice-bath. The reaction mixture was then stirred for 36 h at room temperature, after which the mixture was filtered and the solvent was evaporated in vacuo to give a brown oil. This oily residue was purified by column chromatography on silica gel with Hexane:CHCl3(5:1) then EtOAc to give the product as a colorless oil (54.6 g, 86%).
Synthesis of the secondary amine 3. To a solution of allylamine (21.2 g, 372 mmol) in THF (100 mL) was added tosylated triethylene glycol monomethyl ether (2) in THF (50 mL). The reaction mixture was stirred at 50° C. for 24 h then additional allylamine (10.5 g, 186 mmol) was added and stirred for 12 h. The reaction mixture was partitioned between CHCl3 (100 mL) and 2N HCl (100 mL). The aqueous layer was separated and washed with CHCl3 (100 mL×2). To an acidic aqueous layer was added 2N NaOH until the pH reached about 11. The aqueous layer was extracted with CHCl3 (100 mL×6) and the organic layers were combined and dried over Na2SO4. The solvent was evaporated from this solution to give the product as a yellowish oil (14.0 g, 93%).
Synthesis of the tertiary amine 4. To a solution of secondary amine 3 (5.0 g, 24.6 mmol) and triethylamine (2.49 g, 24.6 mmol) in EtOAc (100 mL) was added bromoacetyl bromide (9.93 g, 49.2 mmol) in EtOAc (20 mL) slowly in the period time of 1 h in an ice bath. The reaction mixture was stirred for additional 3 h in an ice bath. To this mixture was added saturated NaHCO3 (20 mL) slowly. The organic layer was separated and washed with NaHCO3 (20 mL) and water (20 mL×2), and then dried over Na2SO4. The solvent was evaporated from this solution to give a pale brown oil. The oil was purified by column chromatography on silica gel with CH2Cl2:EtOAc (3:2/v:v) to give the product as a pale yellow oil (4.0 g, 50%).
Synthesis of the N-protected diamine 6. To a solution of 3,5-dihydroxybenzoic acid methyl ester 5 (10.8 g, 60.0 mmol) and K2CO3 (37.2 g, 270 mmol) in DMF (100 mL) was added N-Boc protected 2-bromoethylamine (35.2 g, 147 mmol) in DMF (20 mL). The reaction mixture was stirred overnight at 50° C. The reaction mixture was then filtered and the solvent was evaporated to give brown oily residue. The residue was dissolved in CHCl3 (150 mL) then washed with water (100 mL×2) and dried over MgSO4. The solvent was evaporated to give a pale yellow oil. The oily residue was recrystallized from EtOAc:hexane to give the product as a white solid (20.1 g, 74%).
Synthesis of diamine hydrochloride 7. Boc-protected diamine 6 (21 g, 46.2 mmol) was dissolved in CH2Cl2 (100 mL) and diethyl ether (100 mL). To this solution was added concentrated HCl (50 ml) and this mixture was then stirred overnight. The solvent was then evaporated to give the product as a white precipitate. This solid (14.2 g, 94%) was then dried under vacuum.
Synthesis of asymmetric G3 dendron 8. To a solution of hydrochloride salt 7 (0.67 g, 2.0 mmol) and triethylamine (2.0 g, 20.0 mmol) in THF (50 mL) and water (50 mL) was added the tertiary amine 4 in THF (10 mL). The reaction mixture was stirred for 2 days at 70° C. To this mixture was added EtOAc (100 mL) then the organic layer was separated. The aqueous layer was washed with EtOAc (100 mL×2) then the organic layers were combined. The combined organic layer was washed with 100 mL of NaHCO3 (aq) and water (100 mL) then dried over Na2SO4. Evaporate the solvent to give brown oily residue. This oily residue was purified by column chromatography on silica gel with EtOAc:MeOH (2:1) to the product as a pale yellow oil (1.1 g, 45%).
The ester functionality in compound 8 can be transformed to carboxylic acid, hydroxamic acid, phosphonic acid, or other bonding groups following standard literature methods. For example, the ester functional group in compound 8 was transformed to carboxylic acid by basic hydrolysis and the carboxylic acid was further transformed to hydroxamic acid by its reaction with hydroxyl amine hydrochloride salt.EXAMPLE 9 Surface Modification of Semiconductor and Noble Metal Nanocrystals with Dendron Ligands
CdSe and CdSe/CdS core/shell nanocrystals were modified with dendron ligand 6 shown in
Fe3O4 nanocrystals were modified with dendron ligands using hydroxamic acid as the bonding group by the surface replacement of the original fatty acid ligands. Fe3O4 nanocrystals coated with fatty acids were synthesized by the suspension of the bare Fe3O4 nanocrystals formed by the co-precipitation of the ferrous chloride and ferric chloride under basic conditions. Typically, Fe3O4 nanocrystals coated with fatty acids were mixed with an equal molar amount of the dendron ligands in CHCl3, and the reaction mixture was refluxed for a few hours. After the reaction was allowed to cool to room temperature, hexane was added to precipitate the resulting nanocrystals, which left the reaction byproducts in the supernatant. The resulting nanocrystals were soluble in a variety of solvents depending upon the terminal groups of the dendron ligands. A similar procedure was also applied for bare nanocrystals suspended in acidic aqueous solution (about pH=2), in which case, carboxylic acid was also be used as the bonding group.EXAMPLE 11 Formation of Box-Nanocrystals through RCM Strategy
The terminal allyl groups of the dendron-nanocrystals were cross-linked to form box-nanocrystals using Grubbs' ruthenium alkylidene catalyst as shown in
The dendrimer-bridging strategy for the formation of box-nanocrystals is illustrated in
The homobifunctional cross-linker, N,N-disuccinimidyl carbonate (DSC, 8 mg) and 4-(dimethylamino)pyridine (DMAP, 6 mg) were added to a 15 mL solution of G3-(thiol)-OH-coated CdSe nanocrystals in dry DMSO. The absorbance at the first exciton peak of the 3.5 nm CdSe nanocrystals solution was 1.26. After the solution was stirred overnight, 2 mL of such solution was added dropwise into 15 mL of DMF solution containing 8 mg G2 dendrimer amine shown in
A similar cross-linking procedure was applied using ethylene diamine or tris(2-aminoethyl)amine, instead of G2 dendrimer amines. The products obtained from these cross-linking methods were different from the products obtained by dendrimer-bridging with diamine or trisamine as the linkage reagents were soluble in the coupling solutions. The resulting cross-linking products were used as references for the stability studies. The resulting nanocrystals showed some improvement of the stability in comparison to the original dendron-nanocrystals.
Depending on applications, the dendrimer used in this approach may be terminated with thiols, hydroxyl groups, ester groups, and the like. The reaction conditions listed above may vary from one type of inorganic nanocrystals to another.EXAMPLE 13 Coupling Chemistry of the Amine Box-Nanocrystals
The amine box-nanocrystals were found to be very versatile for surface coupling reactions. Thus,
Sulfo-NHS-biotin (2 mg) was added to 4 mL of either the core/shell CdSe/CdS or the CdSe amine box-nanocrystal solution in H2O and DMSO solvent (1:1/v:v). The absorbance of the first absorption peak of the nanocrystals in the solution was 2.5. After stirring this mixture overnight at room temperature, the slight turbid solution was centrifuged. The top clear solution was decanted to another vial for the bioassay. The amount of precipitate was found to be dependent on the ratio of biotin to the box-nanocrystals. It was observed that a high density of biotin on the surface of box-nanocrystals made them insoluble in either pure water or a solvent mixture containing water.EXAMPLE 15 Avidin Detection Using Biotinylated Box-Nanocrystals
Detection of avidin using biotinylated box-nanocrystals was carried out by the addition of 10 μl of avidin solution (1 mg/mL in PBS buffer) to an optical cuvette containing 0.5 mL of the biotinylated box-nanocrystals with absorbance around 0.4 at the first absorption peak. A cloudy mixture formed upon the addition and was centrifuged for 5 minutes, the PL intensity and UV-Vis absorbance of the upper clear solution were monitored. The addition of avidin, centrifugation, and monitoring procedure was repeated until no more precipitate was formed. The precipitate of avidin-biotin/box-nanocrystal complexes was denaturized by heating to about 70° C.EXAMPLE 16 Synthesis of a Dendron Ligand with Bi-Dentate Bonding Site (FIG. 20)
The synthetic steps of Example 16 are presented in
Step 1. Synthesis of N-tert-butoxycarbonylaminoethylamine (2). Di-tert-butyldicarbonate (10.7 g, 50 mmol) dissolved in CHCl3 (60 ml) was added dropwise to a solution of ethylenediamine (30.5 g, 0.5 mol) in 250 ml of CHCl3 at 0° C. during 3 h with stirring. The reaction mixture was stirred for 16 h at room temperature and washed with water. The organic phase was dried over Na2SO4, evaporated to dryness in vacuum to obtain 8.0 g of a colorless oil which was purified by silica gel chromatography (CHCl3—MeOH 5:0.1). 1H-NMR (CDCl3): 1.17 (2H, NH2), 1.39 (9H, 3CH3), 2.74 (t, 2H, CH2NH2, J=6.0 Hz), 3.10 (q, 2H, CH2NH, J=5.5 Hz), 4.99 (bs, 1H, NH). IR (neat): 3354 (N—H), 2977, 2933 (C—H), 1693 (C═O) cm−1.
Step 2. Synthesis of N,N-bis-(2-cyanoethyl)-N-tert-butoxycarbonyl ethylenediamine (3). To a solution of the amine 2 (3.2 g, 20 mmol) in a large excess of acrylonitrile (50 ml, 760 mmol), glacial AcOH (2.3 ml, 40 mmol) was added and the mixture was refluxed for 24 h. The excess of acrylonitrile was evaporated in vacuum to obtain a residue, which was dissolved in CHCl3 (50 ml) and added to 30 ml of concentrated ammonium hydroxide. The organic layer was separated, washed with water and dried over Na2SO4. After evaporation of the solvent, 4.3 g of yellowish oil was obtained. The oil was purified in silica column chromatography with elute (CHCl3—MeOH 5:0.1). 1H-NMR (CDCl3): 1.42 (s, 9H, 3CH3), 2.47 (t, 4H, 2 CH2CN, J=6.6 Hz), 2.65 (t, 2H, N—CH2CH2NH, J=6.0 Hz), 2.87 (t, 4H, 2 CH2N, J=6.0 Hz), 3.18 (q, 2H, NCH2CH2NH, J=6.0 Hz), 4.97 (bs, 1H, NH). IR (neat): 3348 (N—H), 2977, 2947, 2836 (CH2), 2247 (CN), 1692 (C═O) cm−1.
Step 3. Synthesis of N,N-bis-(3-aminopropyl)-N-tertbutoxycarbonyl ethylenediamine (4). The 1.33 g (5 mmol) of compound 3, 25 ml of the 1.4 M solution NaOH in 95% EtOH and 1 g of Ni-Raney slurry in water (pH=10.0) were added to a 250 ml hydrogenation vessel. The mixture was placed under hydrogen (40 psi) in a Parr hydrogenation apparatus and shaken for 24 hours at room temperature. The mixture was carefully in vacuum filtered through sintered glass funnel and the catalyst was washed with 95% EtOH. After diluting the filtrate with 20 ml H2O, EtOH was evaporated and the residue was concentrated. After addition of 20 ml of NaOH saturated solution, the amine 4 began to separate as an oil which was extracted with CHCl3. The combined extract was dried over Na2SO4 and the solvent was evaporated to obtain a colorless oil (1.37 g, 5 mmol). 1H-NMR (CDCl3): 1.20 (bs, 4H, 2NH2), 1.39 (s, 9H, 3CH3), 1.52 (m, 4H, 2 CH2CH2CH2), 2.45 (t, 6H, CH2N, J=7.1 Hz), 2.67 (t, 4H, 2CH2NH2, J=7.1 Hz), 3.13 (q, 2H, NCH2CH2NH, J=6.0 Hz), 5.22 (bs, 1H, NH). IR (neat): 3371 (N—H), 2973, 2933, 2865 (CH), 1687 (C═O) cm−1.
Step 4: Synthesis of 4-cascade-N-tert-butoxycarbonyl ethylenediamine [2]-(1-azabutylidyne)-propanitrile (5). The procedure is similar to that in step 2. The compound 5 was a yellowish oil. 1H-NMR (CDCl3): 1.42 (s, 9H, 3CH3), 1.59 (m, 4H, 2CH2CH2CH2), 2.46-2.56 (m, 18H, 9CH2N), 2.82 (t, 8H, 4CH2CN, J=6.6 Hz), 3.14 (q, 2H, CH2NH, J=6.0 Hz), 4.99 (bs, 1H, NH). IR (neat): 3377 (N—H), 2963, 2935, 2825 (C—H), 2247 (CN), 1702 (C═O).
Step 5: Synthesis of 4-cascade-N-tert-butoxycarbonylethylenediamine [2]-(1-azabutylidyne)-propanamine (6). The procedure is similar to that in step 3. The compound 6 was a colorless oil. 1H-NMR (CDCl3): 1.40 (m, 17H, 9H, 3CH3, 8H, 4NH2), 1.52 (m, 12H, 6CH2CH2CH2), 2.38 (m, 18H, 9CH2N), 2.68 (t, 8H, 4CH2NH2, J=6.6 Hz), 3.13 (m, 2H, CH2NH), 4.93 (bs, 1H, NH). IR (neat): 3375 (N—H), 2939 (C—H), 1690 (C═O).
Step 6: Synthesis of Compound 7. To the stirred mixture of the amine 6 (1.0 g, 2 mmol) in 50 ml THF, 2.0 g of K2CO3 and 1.93 g of allyl bromide (16 mmol) were added and stirring was continued at room temperature for 36 h. The solution was filtered and washed with CHCl3. After evaporating the solvent, yellowish oil was dissolved in water, adjusted to pH, 8 with NaHCO3 solution, and extracted with CHCl3. The combined extract was washed with 10% brine solution, dried over Na2SO4 and concentrated to afford 1.0 g of a yellowish oil, compound 7. 1H-NMR (CDCl3): 1.39-1.54 (21H, 3CH3, 6NCH2CH2CH2N), 2.36-2.47 (26H, 13NCH2), 3.05 (t, 16H, 8NCH2CH═CH2), 3.12 (2H,NHCH2), 5.10 (t, 16H, 8CH═CH2), 5.76-5.82, (m, 8H, 8CH═CH2). IR (neat): 3378 (NH), 3075 (CH═CH2), 2947, 2804(CH2), 1704 (C═O), 1642(C═C)
Step 7: Synthesis of Compound 8. To the compound 7 (1.0 g, 1.2 mmol) dissolved in 10 ml of CH2Cl2, 10 ml of ethyl ether and 5 ml of concentrated HCl were added. After stirring for 4 hours at room temperature, the solvents were removed under vacuum. The resulting residue was dissolved in NaOH and extracted with CH2Cl2. The combined extract was dried over Na2SO4, filtered, and concentrated in vacuum to obtain 0.7 g of an brownish oil. 1H-NMR (CDCl3): 1.52-1.54 (12H, 6NCH2CH2CH2N), 2.2-2.5 (26H, 13NCH2), 3.03-3.06 (16H, 8NCH2—CH═CH2), 3.3 (2H, NCH2CH2N), 4.16 (2H, NH2), 5.08-5.10 (16H), 8CH═CH2), 5.77-5.79 (8H, 8CH═CH2). IR (neat): 3389(NH2), 3075 (CH═CH2), 2946, 2804 (CH2), 1642 (C═C)
Step 8: Synthesis of Compound 9. The procedure is similar to that in step 2.
Step 9: Synthesis of Compound 10, the targeted dendron ligand. The procedure is similar to that in step 3.
All of the publications or patents mentioned herein are hereby incorporated by reference in their entireties. The above examples are merely illustrative of the present invention, and are not intended to limit the scope of the appended claims.
1. A method of forming a dendron box comprising:
- contacting a solid nanoparticle core between about 1 nm and about 100 nm in diameter with a plurality of cross-linkable dendron ligands to form a dendron-nanoparticle;
- cross-linking the dendron ligands to form a box-nanoparticle; and
- removing the solid nanoparticle core from the box-nanoparticle to form the dendron box.
2. The method of claim 1, wherein the nanoparticle core comprises a nanocrystal.
3. The method of claim 1, wherein the cross-likable dendron ligands are selected from G3-(hydroxamide)-CH═CH2, G3-(carboxylic)-CH═CH2, G2-(amine)-ester, G3-(thiol)-CH═CH2, G3-(diamine)-OH, G3-(thiol)-OH, G3-(dicarboxylic)-OH/PEG, or G4-(dicaboxylic)-CH═CH2/PEG.
4. The method of claim 1, wherein dendron ligands comprise cross-likable alkene groups prior to cross-linking, and wherein the alkene groups are substantially cross-linked by a ring-closing metathesis reaction.
5. The method of claim 1, wherein the dendron ligands comprise cross-likable groups prior to cross-linking, and wherein the cross-likable groups are substantially cross-linked by a dendrimer bridging reaction using a linker molecule.
6. The method of claim 1, wherein the nanoparticle core comprises a metal, an elemental non-metal, an organic compound, a metal oxide, a metal chalcogenide, a metal pnictogen, a metal halide, an alloy, a mixed metal oxide, or a combination thereof.
7. The method of claim 1, wherein the nanoparticle core comprises a boride, a carbide, a suicide, a nitride, a phosphide, an arsenide, an oxide, a sulfide, a selenide, a telluride, a fluoride, a chloride, a bromide, an iodide, or a combination thereof.
8. The method of claim 1, wherein the nanoparticle core comprises an oxide of silicon, aluminum, titanium, zirconium, iron, nickel, cobalt, manganese, ruthenium, antimony, tin, cerium, barium, vanadium, chromium, lead, copper, indium, yttrium, zinc, mixed oxides thereof, or combinations thereof.
9. The method of claim 1, wherein the nanoparticle core comprises a CdSe nanocrystal, a CdS nanocrystal, or a CdSe/CdS core/shell nanocrystal.
10. The method of claim 1, wherein the nanoparticle core is removed from the solid nanoparticle core is removed from the box-nanoparticle by chemical etching, photochemical etching, thermolysis, or a combination thereof.
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|February 5, 2002
|Oldenburg et al.
|June 10, 2003
|Bawendi et al.
|August 19, 2003
|Bawendi et al.
|September 9, 2003
|Bawendi et al.
|December 26, 2006
|Peng et al.
|June 6, 2002
|Peng et al.
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|09 977 212
|February 2000
- Daniel et al., Gold Nanoparticles Contaiing Redox-active Supramolecular Dendrons that recognize H2PO4—Chemical Communications, No. 19, Sep. 18, 2001, p. 2000-2001.
- Kim et al., "Novel Dendron-stablized Gold Nnanoparticles with High Stability and Narrow Size Distribution," Chemical Communications, vol. 7, No. 7, Mar. 21, 2001, pp. 667-668.
- Wang et al., "Dendron-Controlled Nucleation and Growth of Gold Nanoparticles", Angewandte Chemi International Edition, vol. 40, No. 3, Feb. 2, 2001, pp. 549-552.
- PCT Search Report, corresponding to PCT/US03/31461, mailed on Feb. 28, 2005.
- Derwent Publication #XP002262148, Sep. 18, 2002, Abstract.
Type: Grant
Filed: Oct 3, 2003
Date of Patent: Sep 25, 2007
Patent Publication Number: 20050004293
Assignee: The Board of Trustees of the University of Arkansas (Little Rock, AR)
Inventors: Xiaogang Peng (Fayetteville, AR), Haiyan Chen (Fayetteville, AR), Wenzhou Guo (Cupertino, CA), Y. Andrew Wang (Fayetteville, AR)
Primary Examiner: Porfirio Nazario-Gonzalez
Attorney: Kilpatrick Stockton
Application Number: 10/679,247
International Classification: C08K 3/30 (20060101); H01L 21/76 (20060101); C01B 21/064 (20060101);


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Travel Icon London - Pen and Ink Sketch Print
Regular price
Simple, class travel sketch by Seattle mixed media artist and calligrapher Caitlin Dundon with painted touches in butter yellow and beige/white painterly background with calligraphy, "London." 12" x 12". Shipped flat. Available for licensing.
Interested in learning calligraphy or mixed media painting techniques? Find out about Seattle calligraphy classes or Seattle mixed media collage classes taught by Caitlin Dundon. Or take a workshop from Caitlin at an artist retreat in a city near you.

subset: commoncrawl


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Pink & Pearl
This flower arrangement has pink, white and peach ranunculus.
The elegant mix of ivory and bright pink blooms in this Bouq satisfies any mood, from playful to polite, making it the ideal gift for yourself or someone who deserves a little extra attention.
Please note that Ranunculus are smaller blooms that take time to open. For a fuller flower arrangement, we recommend a Deluxe size or larger.
Know Your Roots Meet the farmer
Farmer David
Farmer David lives on the farm. No, literally his house is located right on the farm! Over 30 years ago, David made his way to South America and married an Ecuadorian woman. Together they started growing flowers, and their farm grows world-class ranunculus in every color you can think of!
Learn More about how our flowers are grown


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If you can place yourself in the enviable position of applying for a mortgage for your own home, count your blessings. Credit is tight right now and current homeowners can typically only refinance their mortgage loan. They certainly don't have any chance of selling their home at a profit.
Banks Are in Hot Water
A few bad apples can certainly ruin a harvest. For many years, the banking community pushed adjustable rate mortgages on applicants that could ill afford any mortgage at all. These shenanigans set off the housing market meltdown that is responsible for starting the global economic chaos.
Take care and due your homework to accept the best situation possible. A source you can trust should explain the differences between adjustable and fixed rate mortgages.
The Adjustable Rate Loan
If short term occupancy is in your plans, an adjustable loan might work well for you. However, if you are planning to occupy the property for many years, a fixed rate mortgage is the way to go. Adjustable rate mortgages tend to wreak havoc with household budgets.
Variations on adjustable rate loans are now available. Among them are 3/1, 5/1 and – recently – the 7/1. These two numbers notated together is an indicator that the mortgage loan is adjustable. The first number tells you how many years your interest remains fixed. The second number, explains how often your rate adjusts after the fixed rate ends. Term describes how many years the mortgage payments last, which could be 10 years or as much as 40 years.
The Fixed Rate Loan
A fixed rate mortgage is the safest loan to have when you will be living with a mortgage for a long time. Your budget will love you for rejecting adjustable rate mortgage payments over the length of the loan.
Fixed rate loans are usually more difficult to obtain. Many years ago. they were considered the crème d la crème of the loan world. When the housing market was soaring and fixed rates were higher, lenders saw the adjustable rates as a boon to those who could not qualify for fixed rate mortgages.
Banks knew they could sell the lower up-front payments to these marginal borrowers. Adjustable rates are affordable initially, but if a negative life event strikes, the homeowners without emergency money will be first to lose their home to foreclosure.


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The English Lake District is well-known for its water and its mountains, but maybe less known for its gardens. And yet this "most beautiful corner of England" contains some magnificent gardens of many different varieties.
I recently came across a book that I'd missed noticing when it was first published. (Obviously my eyesight must be going in addition to my hearing, as apparently it has been well displayed in bookshops – and not only in Cumbria).
Gardens of the Lake District, by Tim Longville, is published by Frances Lincoln, 2007 [ISBN: 0711227136] and is a book not to be missed by anyone with an interest in either Lakeland or gardens. It is superbly illustrated with pictures of some magnificent gardens, many of which are open for visiting by the general public.
Next time you're planning a visit to the Lakes use this book to help choose which gardens to visit, and if you're too far away to visit in body let the book transport you there in mind. Click on the image to buy from Amazon.
– David Murray –


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Warning: This article contains story spoilers.
Recently, hackers or data miners were able to discover evidence of missing content from Metal Gear Solid V: The Phantom Pain.
"Users in the Facepunch forum discovered not only a texture of The Boss from Metal Gear Solid 3, but they discovered an entire missing chapter.
MGSV: TPP contains two chapters, the latter of which is a wreck of unfinished plot points and re-used content. Facepunch found a title card that references a third chapter, tentatively titled "Peace"." – Jez Corden, windowscentral.com
It is common for game developers to cut content, but players have expressed that the games story is incomplete. Perhaps the added content would have made the story more whole.
Months ago, Director Hideo Kojima and videogame company Konami had a falling out. Weeks ago, Kojima productions was disbanded and Kojima is due to leave the company after completing work on the game. Unfortunately, development of the next Silent Hill video game, called Silent Hills, directed by Kojima and film director Guillermo del Toro, was cancelled by Konami, as well. With Metal Gear Sold V, it appears the game might have been cut short due to Kojimas' relationship with Konami.
A rough demonstration, of the missing content was shown on the Metal Gear Sold V documentary, which came included with the Collectors edition of the video game. A video posted by youtube user Inzaa shows part of a final battle with one of the main characters on a new level. Concept art and footage for this mission called "Kingdom of the flies" can be seen here:
MGSV:The phantom pain has a save data transfer that was supposed to allow players to return to one of the levels from last years prologue game, Metal Gear Solid V: Ground Zeros.
Director Hideo Kojima stated:
"People who own GZ will be able to play a different mission(s) at Camp Omega in TPP. That's the current plan. There's something of a big feature involved. It's never been done in the games industry before." –www.metalgearinformer.com, November 4, 2014
Apparently, Camp Omega was also cut from MGSV:TPP . Users from a www.gamefaqs.com forum topic titled: So returning to camp omega was a lie?, have comfirmed the level was not included. Additionally, certain enemy A.I features, promised by the games developers, were not present.
Forum contributor AnnihilatorSol on www.gamefaqs.com posted
"Yeah it's a lie, so is the "enemy adapts to your play style"
They only adapt to your techniques for wearing gas mask, but the riot gear pretty much stops everything anyway. The will use flares regardless if you played the whole game on day time, they will wear helmet once you reach mission 9 I believe regardless if you are going CQC only. They wear body armor a lot in later stages, and then riot gear starts appearing around mission 30 even if you are going non lethal."
It is disappointing to see missing content from a game and to know that it could have been better. There is still hope in the form of Down-loadable content. Game developers have the habit of releasing DLC months after a games release. Maybe the missing levels will eventually become available to play."


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Medical Specialty >> Gynecology
Doctors Lounge - Gynecology AnswersBack to Gynecology Answers List
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Date of last update: 10/14/2017.
Forum Name: Obstetrics
Question: Intrauterine Growth retardation
|Shykm - Thu Jan 08, 2009 6:23 am
I am Shyla. 23 years old. 54Kg weight. Married, for 2 years. In my first pregnancy, had a sad experience. I suffered from Intrauterine Growth Retardation which resulted in still birth. This happened in Sep 2007. It was diagnosed due to placental insufficiency. Consequent to this, doctor here advised us rest for one year and asked us to go for regular doses of Aspirin when we plan next pregnancy.
On account of transfer of my husband, we have moved to a new place. Now we are planning for the pregnancy. Whether IUGA may repeat again this time? What are the chances? Could you please advise us what precautions to be taken to prevent this happening again. Should we go for Aspirin dose. Are there any disadvantages of Aspirin, how long that need to be taken and what dose. Are there any other measures to be followed. Pls come back
Look forward your kind advise
|Debbie Miller, RN - Fri Jan 09, 2009 10:48 pm
Of course your doctors will be monitoring you for this condition in a future pregnancy just in case but I would not expect the same thing to happen again. It is probably just due to the way the placenta and cord developed and next time it will likely do well. The aspirin suggestion is for the possibility that a small blood clot may have been involved in the insufficiency of nutrient transfer to the baby. I see no problem with going for the low dose aspirin therapy that is often prescribed in such cases as this. It would not be a full adult dose; just about the equivalent of a "baby" aspirin.
Good luck with your plans.
|Shykm - Sun Feb 01, 2009 3:34 am
Dear Debbie Miller
Thank you for the advice.
As we are planning for the second pregnancy after IUGA in the first, we had an appointment with our doctor here. She recommended us some tests prior to trying for pregnancy and at the same time prescribed Aspirin at low doses. We did the same. We are yet to show the results to the doctor, as she is not available for next one week. The Test / Results are as follows...
1. Lupus Anticoagulant - Negative (ratio of LA Screen to LA confirm is 1.02)
2. Anti Cardiolipin Antibody IgG - Negative
3. Anti Cardiolipin Antibody IgM - Moderately Positive (35 MPL)
4. Anti Phospholipids IgM - Negative
5. Anti Phospholipid IgG - Negative
6. Factor XIII - Normal
7. Rubella Antibody IgG - 279 IU/ML.... A result greater than 10 IU/ml is considered positive
8. Protein C Clotting - 95 % (Reference range = 72 to 160%)
9. Protein S Clotting - Total Protein S - 70% and Free Protein S is 55%
10. Anti-D/S DNA (Immunofluorescence Method) - Negative
11. Anti Nuclear Antibody - Negative
Our doctor also did mention that, in case the results are positive, its better to go for some daily s/c injection of something (i could not make out the name) all through the pregnancy in addition to aspirin..which may be quite expensive.
We would definitely follow the instructions / prescriptions of our doctor. I request you or any other qualified person in this aspect to comment or give your views and advice based on these results.
I look forward to your valued comments.
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At War with the Army
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Rated R for Randy.
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It's a great opportunity for kids to look up from their devices & stimulate their creativity. We have bead workshops, comic workshops, craft corners & more!
Nothing compares to the magic of a good story & we have a whole festival of stories to be shared / listened to / experienced, plus heaps of authors reading & talking about their award winning books.
It's FREE fun for the whole family!
. Did we mentioned our
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text: Vastavam web: Tommorow is going to be a litmus test for four films at the box office. The films Kavacham, Subramaniapuram, Next Enti, and Subhalekhalu are going to hit the screens amidst decent expectations. The prime reason for the release of all these films is a holiday. But if we observe the latest political scenario in Telangana, the scathing effects of the ruling party on opposition by arresting Revanth Reddy at 3.am in the morning and also the grand alliance Mahakutami is working real hard in all the possible ways to make big in the elections. If the people turn out in large numbers on that day, it will definitely show a great impact on the first day collections of the film. And also on the 11th of the month, the elections result will be declared and the entire state will be sticking onto the television sets for the entire day.


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We supply a range of waterproof labels for use outdoors or in damp conditions.
Waterproof Labels
Quite often labels are used in damp, wet, cold or even freezing conditions. In these circumstances a normal paper label might become soggy and become unreadable or even fall off. Waterproof labels can cope with these conditions and stay in place longer.
Label Material
Waterproof labels are made from polyester and as such are tear resistant as well as being resistant to weather, water and oil. Polyester is ideal for being used in laser printers, digital printing and photocopiers, so great for use in your own office or warehouse.
Label Sizes
There is a good range of polyester labels that can be used in your standard office laser printer. All of the labels come on A4 sheets, so no adjustment to your paper tray is necessary.
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1 label per sheet – 210 x 297mm
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Waterproof Labels For Sport
Many outdoor sports such as cycle sportives and triathlons use weatherproof labels. Our waterproof labels stick to helmets, bikes or other equipment that is part of the event. Under normal weather conditions like rain, snow and wind, they will stay in place until removal is required.
The ideal size for waterproof labels for a bike helmet are 99×38.1mm (14 per sheet). This size gives enough space for a race number of 3 or 4 digits long.
1 per sheet labels (210x297mm) can be used for the seat stem on a bike, by being printed several to a page. They can then cut into strips that will be 210mm long, meaning the width can be bespoke to any size that you need.
Waterproof Garden Labels
Garden centres use weatherproof labels to label plants or items that are kept outdoors. The labels are resistant to all weather conditions such as rain, frost, snow and ice (-20 to 100 degrees). They can be printed using a standard office laser printer, so there is no need to have them outsourced.
With the smallest label being just 38.1×21.2mm (65 per sheet), they are an ideal size for pricing labels. The larger sizes such as the 8 per sheet (99x68mm) can be used for product information as product descriptions or logos..
Industrial Waterproof Labels
Lots of manufacturing companies use waterproof labels to label things such containers or equipment. Barrels or plastic containers that are stored outside don't lend themselves to normal paper labels. Paper labels will become soggy and either fall off or disintegrate, whereas polyester labels can last for years.
Avery Weatherproof Labels
Avery make a small range of weatherproof labels that are available in convenient packs of 25 sheets. These are designed to go through any normal mono or colour laser printer. Avery laser labels can be delivered the next day across the UK or even the same day in Manchester.
Waterproof Labels In Manchester
If you require labels quickly, we stock some of the popular sizes for fast delivery in and around Manchester. We can deliver further afield on a next day basis, so contact us on 0161 429 8118 if you need an urgent delivery.