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- About Us
- Our Work
- Our Resources
- Our Partners
Ken Johnson, executive director of Northside Institutions Neighborhood Alliance (NINA), believes homeownership is a powerful catalyst for neighborhood revitalization. People who own a home make a big investment. They take pride in it, work to maintain it, get to know their neighbors and collaborate to make the neighborhood stronger.
"When NINA began, there were 49 abandoned buildings in a square mile, and blight begets blight. Our restored homes created pride of place, generating interest and momentum. This multiplies the positive impact by increasing the value of other homes in the neighborhood, enabling families to build equity and achieve the American dream." - Ken Johnson
Asylum Hill, in the middle of Hartford, was a very desirable neighborhood of stately Victorian homes for insurance and manufacturing employees in the 1800s. Over time, the community lost investment and population, and suffered physical decline. Home to several large corporations and institutions, the revitalization of the neighborhood is important to residents and corporations alike. NINA's partner corporations know many of their employees value Asylum Hill's proximity to work, with easy access to downtown's culture and dining options.
View this success story in a printable PDF format.
When and where: Since 2003, NINA has worked in the Asylum Hill neighborhood. Its homes are clustered on Ashley, Garden and Sargeant Streets, as well as on Huntington and Imlay Streets.
What: NINA has renovated or built 20 homes and one mixed-use building, for a total of 31 units of housing and 5,805 square feet of commercial space. They operate programs for home improvements, landscaping, streetscapes, community safety and youth development. Their Homeownership Incentive Program helps employees of Asylum Hill institutions purchase homes in the neighborhood. NINA also works extensively with volunteers from area corporations and institutions.
Why and how: LISC participates in NINA's work with loans for acquisition, predevelopment and construction. LISC also provides capacity-building and operating support grants, technical assistance and training. NINA was a winner of the LISC Safety MetLife Foundation Community-Police Partnership Award.
Who: NINA's major funders for homeownership development include Aetna, The Hartford, Saint Francis Hospital and Medical Center, Webster Bank, LISC, City of Hartford, Eversource, People's United Community Foundation, Travelers, William and Alice Mortensen Fund, Connecticut Department of Economic and Community Development, Connecticut Natural Gas and Bank of America.
Thanks to the funders who support LISC's work in Hartford:
Bank of America
Beatrice Fox Auerbach Foundation
Bissell Foundation
Charles Nelson Robinson Fund
Citizens Bank
City of Hartford Connecticut Department of Economic & Community Development
Connecticut Housing Finance Authority (CHFA)
Ensworth Charitable Foundation
Farmington Bank
First Niagara Bank
Fisher Foundation
The Fund for Greater Hartford
George and Grace Long Foundation
Hartford Foundation for Public Giving
JP Morgan Chase
People's United Community Foundation
Santander Bank
TD Charitable Foundation
U.S. Department of Housing & Urban Development
United Way of Central and Northeastern Connecticut
Webster Bank


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Industrial Videoscope can be divided into three categories:hard rod,fiber obtic videosope and video borescope.In general,video borescope are the developing trend and the application is very large.
According to different inspection requirements,we need to choose the most suitable videoscope based on endoscope probe diameter,length,articulation ability,visual clarity,focal length and other technical indicators.However,because videoscope is allways used in complex environment,we should also need to consider the abilities such as waterproof, oil proof, corrosion resistance and wear resistance of consideration and so on.
Reference for Industrial Videoscope Choosing:
1. The guiding ability of industrial videoscope.Coantec videoscope is 360°all-way articulation.
2. The color reduction of the industrial endoscope.
3. The lighting of the industrial endoscope.Coantec has 7 levels of lightness,up to 100,000 lex.
4. The imaging clarity of the industrial endoscope.Coantec camera resolution is high definition,up to 1,000,000 pixel.
5. The material of the industrial endoscope.Coantec videoscope probe is made up of titanium alloy.
Coantec videoscopes are mainly applied in special equipment inspection,electric power,car manufacturing,automotive aftermarket,petrochemical, machinery, aviation, pipeline container checking, security checking.Coante,as a mature brand in China,is very popular in world wide.Coantec,Coantec,creating the most user-friendly videoscope.
Service Online

subset: commoncrawl


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Geraldine Sullivan
Specialises in:
Astrology, Clairvoyance, Tarot Cards
Thirty minute reading: £80
One hour reading: £150
One Hour Combined: Healing & Reading £150
One hour Healing: £130
Available: Wednesday
All readings are available in person and via Skype or Telephone
Geraldine's readings are accurate with powerful and moving messages for her clients. Would you like to
know more about a loved one? Do you have unanswered questions and need answers? Or do you need direction? Find out what is in store for you over the next year with inspiring insight and progress forward in your life with confidence. She has over 30 years experience of reading for celebrities, entrepreneurs and people from all walks of life, helping people heal the past for a more calming future.


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Are you an Apostate looking for something fancier than a piece of wood? Look no further! This Magister's Staff is far more impressive than a chewed-up old stick, measuring 68″ long and weighing in at a little over 4lbs.
This kit comes with both the large staff casting as well as a colored translucent gemstone of your choice pressure cast in tinted Smooth-Cast 325 resin. The main staff is cast from Smooth-Cast 320 urethane and has a 48″ long 5/16″ steel bar embedded into the length of the handle for added rigidity.
Gemstones are held in place with the clamping pressure of the jaws, and have small divots marking their orientation. The seam lines on this piece are very fine and only minimal clean up will be needed before primer and painting. For a really bright shine, I recommend polishing the gem with automotive wax before inserting it into the jaws. Please note which gem color you would like in the notes on your order!
This kit will require sanding of seam lines and light filling in some areas. Buyers familiar with prop kits will find this easy to assemble with a minimal amount of prep work needed before finishing.
Finished photo is of our own completed kit and is provided for reference only; if you would like more information about how this piece was made, as well as information on how to take it from its current state into a finished piece, please see our post on our blog!


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|Mrs. Muller -Grade 2
On February 12th, we will have a Valentines Party at 12:30pm. We need items to create an amazing ICE CREAM Sundae party. Please sign up below and don't forget to read ALL the posts. We need parent volunteers to help. Our Valentine's list is below:
Tarver PTO is presenting a "Health and Wellness" Night on Jan. 21st at 6:30 pm. How do you stay healthy?
JOIN US on January 21st 6:30-8:00 for our next PTO Parent Community Night.
Mr. Trujillo and the PTO will give a brief address about current events but the main focus will be HEALTH & WELLNESS!
BRING THE KIDS!! They'll get to show you what they're learning in gymnastics, we'll have family games & activities (so bring your comfy shoes!) and we'll have great nutrition information including healthy and affordable meal and snack ideas.
Staying fit can be FUN!! See you then!
Our new read aloud is The One And Only Ivan. Can you share something interesting or something you are wondering about in the story? Here is a short video.
Individual Student Blogs
May 2014


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Fil Lumière is a technological innovation on the part of Montagut, symbol of the brand for fifty years. This unique material combines the brilliance and softness of silk with advanced technical properties which include high strength and ease of maintenance.
Montagut became Maison Montagut in autumn-winter 2018. Montagut, a family knitwear brand since 1880, is repositioning itself by expanding its knit expertise with a wider range of products. You will find below the different lines to discover.


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New Production
Since the last post, I had our baby! My husband & I welcomed a baby girl into our family and we couldn't be happier (photo below taken by good friend and our photographer, Carey Kirkella). While I'm still working, I'm having to adjust to the new, sometimes random schedule. But rest assured, our online shop is still open and all orders are going out on time.
In addition to that, our Annie Duffle is currently in production at our NYC manufacturer. We're restocking classic black and introducing a new color we have yet to name- it's a perfect grey/taupe that we fell in love with! Everything should be in stock within the next two weeks so stay tuned. Or better yet, sign up for our newsletter and be the first to know when we drop new styles/colors- plus, you'll receive 10% off your order for signing up!


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We are Experts in Intelligent
Telesales and Marketing
Established in 2001, Trifle Solutions has grown year on year providing flexible telemarketing and telesales services specifically for the B2B marketplace.
We hand pick professionals who are driven, self-motivated and most of all love the work they do. To enable our staff to reach your goals they must gain an in-depth knowledge of your business, its products and services, your competitors and the marketplace – all we ask is for your input.


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Insights: arf
Re:think 2015: Searching For Truths in the Analytic Age
The ARF has a rich heritage and is keen to celebrate it. It's also an organisation with an eye on what's coming next – the ghost of research future. Re:think 2015 looked to reconcile old and new.Tom Ewing
The ARF Captures Our Marketing And Research Industry Journey
The ARF Re:Think 2014 conference did a great job of capturing our marketing and research journey. The keynote address on day one was actually an interview of one of the real 60s "mad men": Keith Reinhard, Chairman Emeritus of DDB.Joel Rubinson
Why We Need Cross Platform Media Measurement… TV Is Now The 720 Pound Gorilla
How important is it to measure multiplatform usage by consumers? Gian Fulgoni, Chairman at comScore suggests that TV is now the 720 pound gorilla—still the biggest and hairiest beast, but maybe not quite as large relative to the other creatures in the jungle as it once was.Joel Rubinson
A Day In The Life Of The Researchers Of The Future
How the combination of GRIT data, mobile ethnography, and the hard work of a lot of very smart people gives us a glimpse into the lives of Researchers of the Future.Leonard Murphy
Who Is Leading Insight Industry Transformation?
From 2008 to 2010 ARF was the industry leader in Research Transformation, with a Research Transformation Super Council (RTSC) led by Susan Wagner of J&J, Donna Goldfarb of Unilever, and Joel Rubinson of ARF.Cambiar
Autumn Is Almost Here: It's Conference Time!
You can tell it's almost Autumn; the days are getting a bit shorter, cooler weather is creeping in and there is a flurry of activity as everyone prepares for many of the big market research industry conferences and events. Here's a rundown of some upcoming events this fall.Leonard Murphy
Viva la Revolucion!
The Research Liberation Front has come up from the underground and are spreading their dissident ideas of "Set Research Free" via the web! Authorities are trying to identify these rabble rousers who dare to try to upset the established order within the MR industry!Leonard Murphy
Lead Up To The IIR TDMR: Interview With Dr. A.K. Pradeep of NeuroFocus
Next up in our series of interviews with presenters at the Technology Driven Market Research event is the Keynote Speaker: Dr. A.K. Pradeep, CEO of NeuroFocus.Leonard Murphy
ARF re:Think 2011: Networking, Clubbing & Revolutionaries
The re:Think 2011 experience reminded me of the importance of taking our online relationships into the real world. I love being able to connect and collaborate with colleagues online. But something special does happens when you shake hands with friends old and new.Leonard Murphy
ARF re:Think 2011: The Great Neuromarketing Debate
One of the most buzzed about topics at the ARF re:Think 2011 was Neuromarketing. Several sessions were devoted to the topic, including the much anticipated "Neurostandards" presentation. In case you have not been following this effort, here is the crux of the matter.Leonard Murphy


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The SHL's new minor league, the Continental Hockey League, has completed its first week of play. So far, there aren't any dominant teams, top-flight goal scorers, or dominant netminders. In general, the league's leaders have yet to emerge… except one.
When it comes to penalty minutes, there's an undisputed leader: Albuquerque Screaming Eagles defenseman Cedric Meloche. In his first five games, Meloche has already racked up 26 penalty minutes, twice as many as his nearest competitor. He has earned that lofty total largely through his fists, as he has already gotten into four fights.
"I like to fight," Meloche admitted cheerfully.
The 20-year-old attributes his professional success to his pugilistic abilities. "When i we were young, we all wanted to be hockey players," said Meloche. "But I was a little guy and could not skate too fast or shoot too good, so I had to fight. I learned to fight good, so I moved up."
It took all of 42 seconds for Meloche to get into his first professional bout against the Minnesota Freeze. When Freeze D "Chilly Willy" Calligan gave Eagles C Vance Ketterman a hard check into his own bench, Meloche took exception and clocked Calligan in the chest, touching off a donnybrook. Late in the third period, it was Calligan's turn to take umbrage after Meloche enthusiastically fouled a couple Minnesota players, and the two wound up throwing hands again.
On Saturday, Meloche against fought twice in the Eagles' game against the Muncie Squirrels. In the first period, Squirrels C Britt Cadmium leveled Eagles RW Ashton Starhawk with a vicious hit that was not penalized. Meloche responded by hauling Cadmium down from behind. Surprised and irked, Cadmium bounced up and stared Meloche down yelling, "You wanna go, little man?" Meloche replied, "Yes, I wish to go!" They proceeded to drop gloves and trade blows, with Meloche bloodying Cadmium's nose before they could be separated.
Two periods later, Meloche and Muncie D Zander Phthalo began jostling vigorously during a faceoff. The jostling escalated to shoving and then to punching, and Meloche wrestled Phthalo to the ground before they were separated by the referees.
After Saturday's slugfest, league officials threatened to suspend Meloche if he continued racking up fighting majors at this rate. Eagles coach Butch Slazenger, recognizing Meloche's value to the team, also counseled his blueliner to rein it in. "I love Cedric Meloche," said Slazenger. "He's my favorite player. And all the guys love that he has their back. But he's not just a goon. He's strong on both ends, and we can't afford to have him suspended. So I told him to pump the brakes a bit. Try not to get into multiple fights in a game, watch out for instigator penalties, stuff like that. Don't give them an excuse to suspend you, because we need you."
Meloche said he will try to heed his coach's advice. "I always play the way I play," said Meloche, "so I will stand up for my team and fight. But I know it is bad if they throw me out, so I will maybe not fight so much. I want to do the best thing for my team."


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As a part of the Enviroschools network, Linton Kindergarten are finding ways to strengthen sustainable practices.
A recent rubbish audit at the kindergarten revealed that paper was a large part of generated waste. Two baskets were added to the art area for paper collection. One basket is for reusable paper, and one for recyclable paper.
The challenge then, was to find possible ways to reuse the paper. A professional development trip to a kindergarten in Havelock North sparked the idea of making new paper from old paper.
The process begins by ripping the paper from the reusable paper basket into tiny pieces. Rainwater, collected from the roof into the water tank (which is another deliberate way of reusing water), is then added. After a few days, the paper is whizzed by a special drill, turning it into a mushy paper pulp, that is then spread onto a deckle (made from recycled picture frames).
The paper is then flipped onto a towel, allowing the excess water to gently roll away. Tamariki work together, taking the new paper to drying racks placed in the sun.
Paper making requires plenty of commitment, perseverance and focus, but is certainly worth the effort. Children are empowered to take part in a process that teaches a practical way to manage some of the waste generated at kindergarten.


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The issue isn't really about the prime minister. It is about the price we pay for speaking up about racism.
Though HRV may play a role in wisdom, Grossman thinks that there isn't a lot one can do to change it--it's more a matter of individual differences. But, he says, we may want to consider training people in impartial, third-person perspective taking to help them be wiser in life, whether they have high HRV or not.
I typically agree with much of what David Brooks has to say. However, I'm afraid that he was way off the mark with his article "How Covenants Make Us." Demographic diversity in the United States is a good thing. The problem has to do with the lack of integration and assimilation of demographically diverse communities.
Law schools must begin recognizing the damage that is being caused to interpersonal relationships and our overall society, due to the low EQ levels of those involved in the field of law and make a concerted effort to address this extremely serious problem.
We are both members of The Esquire Network (TEN) and he has been mentioning that we seem to have very similar approaches to the practice of law and suggesting that we should discuss it further over breakfast. We did just that.
I want to take this opportunity to thank all those involved in the production and development of Disney•Pixar's film "Inside Out." The film is named "Inside Out" because it is about the inner workings of the mind, which controls how people behave.
The fight for equality among all genders, races and sexual orientations requires just that same rainbow of people who want change -- and this includes men.
Empathy is the experience of understanding another person's condition from their perspective. You place yourself in their shoes and feel what they are feeling.
Instead of dictating how young deaf people should live their lives, parents can work together with their child to find the most comfortable way of adapting.
Women are notably under-represented in the fields of Math, Science, Engineering and Tech. Only 5.5 percent to 22.3 percent of civil, industrial, chemical and mechanical engineers are women, and they represent just a quarter of the workforce involved with computer and mathematical sciences.
I'm not talking about an approach where you sugar coat your life experiences so that everything is "really amazing" and every day is "super fantastic." That's a bit nauseating. I'm talking about simply seeing the truthful beauty in life for what it is and trying to show a bit less but.
The false limits we have set up for ourselves can be shattered to reveal the core of our genuine being. You can use the following 10 principles for building the most authentic version of yourself.
Willie nods his head and then says: "Yeah Robert, people make mistakes... that's why pencils have erasers!"
Unless you are on your way to the hospital or living on the street and have nothing to eat, a detour on the road called "your daily life" is not so much a problem -- it's an inconvenience.
Will Perspectives' new status as a leading program affect other area charters? We haven't identified any partners yet, whether
To have this relationship with these two boys is literally the best thing I will ever have. And to always be with them, and for them, in this world, is the highest calling I will ever know.


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5 Quick Tips to Making Shareable Content
Get the Word Out!
Sharing your business's updates, events, and announcements with a chamber of commerce or destination marketing organization (DMO) for inclusion with their email blasts and social media messaging is smart. Here are some tips to make your message quickly shareable by a chamber or DMO, the members, and social media followers.
#1. Keep it short and make it concise.
Put the meat of your message into one paragraph. If your message is long, see tip #5
#2. Think copy and paste.
Make it easy for the recipient to copy and paste your content in their email blast, on their Facebook page, or their blog. Create your shareable content in a Word document or as an email as these formats are easy to copy and paste from. Embed links to websites if relevant.
#3. Include a graphic or image.
Messages are more likely to be read if accompanied by an image or a graphic. The graphic/image should illustrate your content. There are several free online services that allow you to create a free graphic (like the one I created for this article). Try sites like Canva, Pablo, or Adobe Spark. For free images, try Pixabay.
#4. Create an attention grabbing headline.
Tell the world what your message is about in one sentence. Your content is more likely to be read if it has a bold text and with a short, 70 characters or less, message.
#5. Create a blog post on your website.
This is my favorite way of sharing content and will give your business the biggest bang for your efforts. Create a complete message you wish to share on your website as a blog post. Then, create one concise paragraph of your message. At the end of the paragraph, add the words LEARN MORE and have those words link to your complete blog post. This increases traffic to your website and will give your website more SEO power. Winner, winner, chicken dinner.


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Investors are once again on a record pace of purchasing in 2005, matching their pace from 2004. The National Association of Realtors reported in March that "twenty-three percent of homes purchases last year were for investment and another 13 percent were vacation properties."
LoanPerformance, a subsidiary of First American Corporation, reports this year that investors have made up 7.19 percent of all mortgagees through the end of April 2005 -- which is more than a 300 percent increase since 2001, according to a brief in the Wall Street Journal.
The obvious is obvious: despite fears of a bubble, real estate is the hottest investment tool in the 21st century to date. Outside of a real estate meltdown, it appears the advent of retiring baby boomers will be looking for real estate to purchase, both close to home and out of state. Some of those targets are waterfront properties. Since there's only so much coastal property, you may be in competition with buyers/investors from all over the world for land on the water.
The good news is you don't have to be a millionaire to invest in coastal property, but be wise in your selection process. I heard a radio ad the other day touting to residents in the Washington, D.C. region (three states in all) to come to North Carolina to buy waterfront land for as low as $99,900 per lot.
Now, for many would-be investors, purchasing a piece of property without a house on it for a hundred grand is ludicrous. But when you're in a market where a 2-bedroom bungalow goes for $700,000 – well, a lot in another state for $100K seems real affordable. So, I called.
The property in question looks great on the internet. The lots are from a quarter to a half acre in a 600-acre development that's two-thirds sold. By the time the development is complete, there will be a man-made lake, boat launch, community center and canal-front properties throughout.
The sales person even talked about how all the developer's other communities had sold out in just 3 to 6 months. Wow … better get on the appreciating bandwagon while I can, right?
Well, that's the question I put to one of my investment mentors. (I have several.) He owns land in North Carolina, Florida, Hawaii -- plenty of coastal areas. I haven't had the opportunity to purchase coastal land, yet, so I'm hoping this will be my first dive into the land pool. He was excited for me when I described the slick brochure over the phone. And wisely said, "You need to go down there and check it out."
So my chief investment officer (who's also my beautiful bride) and I are heading down there this weekend to check it out -- along with the tips handed to me from my mentor and I pass them on to you:
- Walk the land -- don't buy sight unseen. There are so many things that can go wrong with land. Look it over closely. Get a hold of the site plan -- where are you're lines, what's on your land, what's on your neighbor's land, where are the septic fields, etc.
- Read up on the history of the land development (local newspapers, internet). Was this a friendly undertaking or was there a lot of grief from neighbors? What was on this land before? A farm, wooded land, mobile home park, etc.
- Visit homeowners in the community who have already built a house. What do they think about the developer? Don't be afraid of this. Just go up, knock on the door and ask: "I'm considering purchasing a home at the Lucky Ducky Water Resort next door. How have you liked living here? Was the house built to your satisfaction? Has the community amenities been developed as promised?" The current owners will know and will gladly tell you either good or bad.
- Will the land percolate for the size house you want? Don't buy a piece of land on the coast you can't build on unless you just want a camping/fishing lot.
- If you're excited about owning water front property, go ahead and look at waterfront property in the area you're searching that already has a house on it. Instead of waiting to build, you may be able to get something now that can actually create a cash flow.
- Don't shop just in one waterfront development. Look over developments in the same area and search for land through local MLS search engines.
- Check out the economic growth of the county. Who are the employers? Are they growing? (Seek out this information from the Economic Development Authority -- local or state level). You want to purchase an appreciating investment.
- When was the last drought and what happened to the water levels then? Did the waterfront properties become beach front homes?
Just like any other investment, a waterfront purchase requires due diligence and a commonsense, step-by-step approach to finding out if this is a boom or a bust.
Mr. Carr has covered real estate since 1989. He is the author of Real Estate Investing Made Simple. Got a personal real estate issue? Post your questions and comments at Anthony's blog.


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20/8/2018 1 Comment
Movies to watch on Netflix
As promised, I have put together a blog post of different shows to watch on Netflix for all of your moods. Hope you enjoy💕
When you want to laugh
1. Grown ups
2. White chicks
3. Clueless
4. Norbit
5. Daddy day care
6. Blended
All the rom-coms you need…
1. How to lose a guy in 10 days
2. Two Weeks Notice
3. 27 Dresses
4. Bride Wars
5. The Proposal
6. Miss Congeniality
When you want to cry
1. Dear John
2. The Notebook
27/8/2018 10:16:22 am
Thanks for the suggestions Jenna - took your tips and watched Blended on the weekend - absolutely loved it. Definitely a feel good movie 😊
Leave a Reply.


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Guest Starring:
Elsa (NPC'd by Sydney), Jan (NPC'd by Daphne), and Sol
Date: May 9, 2010
Sometimes you owe, and sometimes you — pay.
"Wages Of Sin"
Sol's Penthouse Apartment
"You procured it, ja?"
"No, I did not. He was there."
"Your father will not be happy with this, huh? What will you tell him?"
"I will tell him…" Vasha pauses as she pulls a tumbler from the bar and fills it half full with an amber liquid. Raising the glass, she curls her hand around until the backs of her fingers touch her lips and chin. It almost seems as though she is hugging the glass for comfort. "I will tell him that the Captain was already there. I will tell him the truth."
"He will not be pleased."
"No, I expect not."
Fourteen Years Ago
Pulling herself from the pool, Vasha's bronze legs take long strides toward the deck chair where her towel lays. Pulling it up, she dabs the water from her face and turns toward the french doors leading to her father's bedroom. Through the glass, she can see Elsa there. Her eyebrow twitches upward in surprise, slowly, she creeps across the patio, hugging the wall until she's adjacent to the door and able to hear.
Elsa glances at the french doors and then back to her cohort. "You are the one. You know you are the one. That's all that matters, ja?" She's not pleading, she's trying to make sense of the situation in which she finds herself. Her straight, long, dark hair is smoothed as she steps towards him to reach out a hand to his arm. "Come, my darling. Can we not put this unpleasantness behind us and move on?" Fortunately, like it or not, her lips rest in a perpetual pout, keeping her thoughts a complete secret to those around her. Her little black dress falls mid-thigh, but rises a few inches further as she takes a step. "Come, have a drink. Scotch? Neat?"
The muscular arm reached for retracts, disgusted with the very idea of a touch from this dark-haired vixen. Jan's entire figure fills the antique chair in which he sits, pressed against the cushions in an unflinching pose meant for the gargoyles on cathedrals. Before he pulled away, both arms were on those of the chair, thick fingers clenched about the ends of the rests with intent enough to leave permanent indents. The dark mood is redundant on the chiseled face, an overcast of grim deliberation making his emotions quite clear, if not his thoughts and intentions. His hand leaving the chair arm slides into his pocket, removes something. Bits of wire and charred plastic clinging to an unrecognizable hunk of material. "Move on," the rumble starts low in his chest as he takes his sweet time answering her. "Move on, she says. Is that what you want me to do, wo-man."
Outside, next to the wall, Vasha crouches low to the ground. The dark, slim figure of one of the servants catches her eye and she turns her head in his direction, narrowing her eyes in a glare. He scuttles into the house on the opposite side of the pool, fearful of raising the young mistress' ire. The death of the Bantu, Dangaan, was not only a slight on the house but a great loss to the warlord. Though not quite indispensable, he was a loyal man and a friend to the young woman skulking to the side of the double glass doors.
Present Day
"What will you do now, huh? You will tuck your tail and return to Beaconsfield to hide under your father's shirt?"
The glass flies across the room to smash against the wall next to the man's head. "Be mindful of your place, Old Man, I have not yet decided my course of action." She turns, waving her hand toward the trembling boy standing against the wall. "Clean that up, be quick." Her sneer of displeasure hurries him along, as though her tone of voice isn't enough to do the job.
"Where are you going?"
"I am going out. The drink here is less than palatable." Or maybe it's the company. She never could stand having his hands all over her.
Fourteen Years Ago
Dark eyes flit towards the alcohol and then back to Jan. "Dahling, you lived did you not?" her arms are drawn to her sides while she shifts her weight, the only sign of her uneasiness about the entire situation. In fact, Elsa has quite the poker face overall — a sea of calm in the middle of a storm. "And what else can we do? What else can anyone do? In my estimation a person can die or move on." Her dark long eyelashes flutter with a kind of odd flirtation — not outwardly flirty, but it's a flirtation regardless. "So. Would you like that drink?" her expression turns stoic, statuesque.
"I will choose who dies and who moves on," Jan's rumble turns into a thunder clap, the arrogance rolling so naturally off of him that it smacks of fact. Half of him rises from the chair, disconnecting from the expensive fabric like a monster birthed from the shadows he's placed himself in. "And I will not tolerate to live any who disrespect this house, like this motherless scum-sucking coward Por-ter." Seething a moment longer, he regains his composure the next. Sliding naturally into place, fitting into that black throne that has become an extension of him. He uses the hand holding the burnt bit of metal, curling it towards himself as guide. "Yes… bring me that drink."
Still crouched outside the door, Vasha slowly straightens to her full height. Though not as thick as her father, she commands an imposing figure when she wishes. Her face hardens and slowly, she steps out from her hiding place, wrapping the towel around her waist to cover herself. Throwing a glare into the glass, her eyes find Elsa and she tenses her jaw. She stands outside the door, knowing Jan will either reward or punish her for what she's done. Disrespecting the woman that he placed in the position of mother to her is a great offense in the house, even still.
She'd answer him, but that's a disrespect in itself. Of course he decides who dies and who moves on, but it's her job to remind him what she does here. Her wiles, her contributions, and the like. She steps over to the scotch service and pours a tumbler-full of the amber fluid into a tumbler. There are servants to do this kind of thing, but currently this is her way of calming him. Elsa gracefully steps back to the place where Jan is seated. "There is no man of your stature inside or outside these walls. You know that, dah-ling." Her tone remains level as does her gaze, almost as if dealing with a lion as its potential prey.
"He is an insect," unlike the lion that is Jan, "And like one he will crushed." Now when Elsa approaches, his hand has reconsidered; thick grasp coming to find her upper arm, caress the skin, pushing away hair as it gets in the way of his touch. As he does, the rough black cut of his eyes find Vasha across the way, the vision of her defiant even in her towel. "And like him…" He continues, nearly to a purr with that low, earthy voice that can just as soon turn to earthquakes— that does: "All who grovel in the dirt by his side!" The caress has turned into a crushing hold, bruising skin but not happy until bones creak as he erupts from his chair, yanking with one powerful sweep to force Elsa into it: cornered. Set on display. A finger curves outwards, stabbing the air towards his flesh and blood watcher at the door. "Come here," he instructs neutrally, storm swept away. "Come and tell me what you think of this woman now."
Stepping through the open door, Vasha moves fluidly to her father's side. The water still dripping off of her from the swim she had just moments before. "Ja, Papa," she emits, her voice calm and level, much like his. Hazel eyes look down at the woman in the corner, then the teenager takes a breath, causing her chest to swell with pride. She wasn't the one who betrayed Jan, she was his savior, his angel. "I do not think of her, Papa, she is dead to me." With a few slow blinks in Elsa's direction, it is as though she no longer exists to the girl. The Bantu is dead, her father's hold on the land crippled by such a display.
A shriek is emitted at the movement — a stark contrast to her proffered emotionlessness moments before. The liquor spills on the floor and the glass shatters against it as Elsa is thrust into the chair, cornered like the prey that she is. There is no stoicism left in the earth. For Elsa there is no mercy; there is no mirth; there is no joy. Terror crosses her features. Her eyes fight to find some semblance of wit or cunning, but none can be found. Her gaze flits to Vasha and she knows there is no words she can utter that will change her former-lover's mind, yet she speaks anyways, her tone fearful, yet firm in its resolve, as an ironic smile spreads across her lips — if she's going to receive retribution, she may as well do all she can to deserve it, "I lied. Kyle Porter is twice the man you are," she glances down towards his crotch, "in more ways than one." And then she turns upwards to meet his gaze. A dare. A terrified dare. Pained yet accepting at the same time. At least she sent him one last blow. At this one wasn't of the job variety.
Angel she may be, but Vasha is not looked upon with any form of on high-generosity; there are only monsters here, and the snake amongst them must be purged. The task brings no pleasure to Jan's heavy features, only a grim set of anger lines amongst the weight of responsibility. This. Is his house. But the first curve of his powerful arm lashes not towards Elsa but his daughter, landing flatly on her cheek with no sense of restraint on account of youth or blood shared. "You will shed a bitter tear for a mother who is so wretched she is not even a woman any longer." Mother is sacred. Elsa is not. He gave her this chance; she spit in his face. And now, at his pride. The pillar of his body does not curve at her insults, though thin lips part cruelly to the side to reveal white teeth in sharp contrast, a sneer he wears too well. "No one will see fit to recognize you. They will flinch and say: what is this hellish creature that does not belong even below our feet." And his arm extends, the length of it a terrible sentence until that hand wraps the entirety of Elsa's face in its grasp. She has seconds within which to imagine what is next.
Vasha's head pivots to the side as she is sent reeling from the slap across the face. Her father is strong, but he knows her resilience, he knows her limits and that is not one of them. Shaking her head, she snorts to clear her head from the dizziness. Then one bronzed hand reaches up to touch the tender cheek as she glances up to her father, an expression of betrayal painted across her face. "Ja, Papa," she utters obediently, though the sadness shed are for her own pain, not for the woman on the floor. Blinking twice, a small rivulet forms from the corner of each eye as two tears rolls down her cheeks. Turning slightly, she faces Elsa, the coldness in her eyes betraying none of the sentiment she once gave the woman she called Mama.
"And no woman in her right mind would take you — " she hisses through clenched teeth before his hand grasps her face — at which a very distinct yelp followed by a glass-shattering scream escape her lips. Her fear is palpable, particularly in the beads of sweat that form along the outside of her skin. But then, it's a fear she's lived in for years; a fear that's washed over her because of the man she knows so intimately yet fears just the same. And perhaps that's the appeal, some deep-seated sadomasochist fear that makes all of it that much more thrilling. Her cheeks are wet with tears for the impending doom — not only that which befalls her, but for the fate of her foster daughter — a fate worse than death; an existence where coldness and detachment reign and where any sign of emotion, remorse, or empathy are considered complete weaknesses. And perhaps, that is the true pain.
Unyielding that grip, unmoving that rock of resentment. When Elsa decides her last words are going to be ones of wickedness, Jan's mouth hardens into an exact line. A moment of serenity for this family; a still-frame of father with false mother in hand, bruised daughter standing by. Then, the hand squeezes. In the same motion, he brings his elbow up and then straight down, rocketing the trapped woman to the floor, her head slamming into the broken shards of the glass of scotch she meant as peace offering mere minutes before. Before she's fully even hit the ground the first time, his hand lifts away, forming a massive fist of curled fingers and thick calloused knuckles that fly forward to impact once — twice — repeatedly into the face of Elsa. An early one breaks her nose, splattering its blood amongst the glass shards. Every one after that cracks something else, rearranging once beautiful features that he would cup tenderly as they shared a bed not paces from this very spot. He continues even after her squirms have long ended: continues to pummel the sin from her.
Present Day
Leaning her head against the steering wheel, Vasha's eyes are closed as she fumbles to insert the keys into the ignition. The moment they are turned, the engine is screaming like a beast to be let loose onto the streets. Opening her eyes, she stares at a brown stain on the concrete barrier. It could be blood, some of the very same that purchased the vehicle, but it's not.
Soon after the request is made, it is granted and the silver Lotus peels out of the parking garage, into the rain dampened streets. The manual states 0 to 60 in 5.5 seconds, it takes Vasha less.


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Leisure Industry print and promotion
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Well we're back! After a non stop summer we're back here to tell you about some of the things we've been up to!
Barnsley Premier Leisure are our longest standing customer. They are a really
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Well we're back! After a non stop summer we're back here to tell you about some of the things we've been up to!
Barnsley Premier Leisure are our longest standing customer. They are a really
Dear all
Things are progressing nicely here at SSL. We've recently been asked to tender for some new design work for the NHS. And I'm proud to say that we've won it!!
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Today we were asked to do a quick theatre mailing. Tickets for an up and coming children's show were NOT selling, so a small budget was allocated to create a direct mail piece. We
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We've recently been asked to get involved in some more charity work for Barnsley Hospital Charity. The brief was to design a charity box to go into schools, to raise money for the 'Tiny
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Barack Obama was appalled- simply appalled by the Blagojevich allegations!
The One insisted:
"I have never spoken to the governor on this subject. I'm confident that no representatives of mine would have any part of any deals related to this seat."
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Obama claimed that his advisors' hands were clean:
Earlier today it was reported that Rahm Emanuel spoke several times with Rod Blagojevich about the open senate seat.
Now, it is being reported that Rahmbo was even giving Blagojevich names of acceptable replacements.
The Telegraph reported:
Sources close to the investigation said Rahm Emanuel, the new chief of staff, had conversations about the issue with Rod Blagojevich and John Harris, the governor's chief aide who is also facing corruption charges.
It is claimed that at one point he even gave them a list of acceptable candidates to take over the vacant seat.
Suspicion has grown that Mr Emanuel is the unnamed "Washington based adviser" mentioned in the 76-page prosecutors' affidavit that Mr Blagojevich ordered his chief of staff to contact.
Mr Obama, who takes office on Jan 20, has vehemently denied any of his staff were involved in deal-making and has promised to reveal any contact between members of his team and the governor's office.
The sources close to the investigation did not say Mr Emanuel had discussed any deals, but even being on receiving end of corrupt demands could be damaging to him and Mr Obama. The sources told Fox News that it was likely the discussions were recorded.
TRENDING: Pelosi Accuses Trump of Killing Americans in Coronavirus Crisis: "As President Fiddles, People are Dying"; Raises Watergate Impeachment Question
So, it looks like besides the list of acceptable names and several conversations, Barack Obama is correct…
His representatives did not take part in any deals related to this seat.
As of 4 PM this afternoon.
Tom Maguire has more thoughts on Rahmbo's involvement.
Am I the only one who is looking forward to hearing these tapes?
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More… Rahmbo comes unglued— He's "beet red" and "pissed" that he can't go anywhere without someone asking question about his role in Blago-gate.
BUSTED!… ILLINOIS GOVERNOR BLAGOJEVICH ARRESTED! (Video)
Blagojevich Calls His Buddy Obama a Motherf***er
Best. Corruptocrat. Headline. Ever.
"Blagojevich Arrest Shakes Obama Presidency to Its Core"
Oh Brother… Obama Already Caught Lying About His Contact With Blagojevich
Obama & Emanuel Worked Closely to Get Blagojevich Elected
Obama Met With Blagojevich in Chicago On Nov. 5 To Discuss Senate Seat
Oh Gee We're All Shocked… Jesse Jackson Jr. Is "Senate Candidate No. 5"
Warning: Bumpy Road Ahead… Blagojevich Gets Tossed Under Bus
Report: Michelle Obama Is "Specifically Named Individual" in Indictment
Change… Blagojevich Held 2 Hour Long Conference Call With Obama Advisors
Ruh-Roh…Rahm Emanuel Continues to Duck Questions On Hot Rod
The Blagojevich-Obama Team Conference Call (Video)
Rahmbo El Busto!… Emanuel Spoke With Hot Rod About Senate Seat… Several Times!
Rendell Blasts Obama Over Hot Rod Scandal


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Søgeord (borrelia) valgt. Opdateret for 11 timer siden.17 emner vises.
Eckman E, Clausen D, Herdt A, et al.
AbstractBackgroundDemonstration of intrathecal production of Borrelia-specific antibodies (ITAb) is considered the most specific diagnostic marker of Lyme neuroborreliosis (LNB). Limitations include delayed detectabilty in early infection and continued presence long after successful treatment. Markers of active inflammation – increased CSF leukocytes, protein and CXCL13 – provide nonspecific markers of active infection. To assess the utility of CSF CXCL13, we measured its concentration in 132 patients with a broad spectrum of neuroinflammatory disorders, including LNB.MethodsCSF CXCL13 was measured by immunoassay. Spearman's rank correlation test was performed to explore its relationship to conventional markers of neuroinflammation and Borrelia-specific ITAb production.ResultsIn non-LNB neuroinflammatory disorders, CSF CXCL13 elevation correlated with CSF IgG synthesis and leukocyte count. In LNB, CXCL13 concentration was far greater than expected from overall CSF IgG synthesis, and correlated with Borrelia-specific ITAb synthesis. Median CSF CXCL13 concentration in ITAb-positive LNB patients was >500 times greater than in any other group.ConclusionsIntrathecal CXCL13 and IgG production are closely inter-related. CXCL13 is disproportionately increased in "definite LNB," defined as having demonstrable Borrelia-specific ITAb, but not "probable LNB," without ITAb. This disproportionate increase may help identify patients with very early infection, those with active vs. treated LNB, or help differentiate ITAb-defined active LNB from other neuroinflammatory disorders. However, its reported specificity is closely related to the diagnostic requirement for ITAb. It may add little specificity to the demonstration of a pleocytosis or increased overall or specific IgG production in the CSF.
Thompson, C., Mason, C., Parrilla, S., Ouyang, Z.
Borrelia burgdorferi encodes a functional homolog of canonical Lon protease termed Lon-2. In addition, B. burgdorferi encodes a second Lon homolog called Lon-1. Recent studies suggest that Lon-1 may function differently from the prototypical Lon protease. However, the function of Lon-1 in B. burgdorferi biology remains virtually unknown. Particularly, the contribution of Lon-1 to B. burgdorferi fitness and infection remains hitherto unexplored. Herein, we show that Lon-1 plays a critical role in the infection of B. burgdorferi in a mammalian host. We found that lon-1 was highly expressed during animal infection, implying an important function of this protein in bacterial infection. We further generated a lon-1 deletion mutant and an isogenic complemented strain. Relative to that of the wild-type strain, the infectivity of the mutant was severely attenuated in a murine infection model. Our data also showed that the mutant displayed growth defects in regular BSK-II medium. Furthermore, bacterial resistance to osmotic stress was markedly reduced when lon-1 was inactivated. When exposed to tert-butyl hydroperoxide, survival of the lon-1 mutant was impaired. In addition, production of several virulence factors such as BosR, RpoS, and OspC was elevated in the mutant. These phenotypes were restored when lon-1 mutation was complemented. Finally, we created a lon-1(S714A) mutant and found that this mutant failed to infect mice, suggesting that the proteolytic activity of Lon-1 is essential for bacterial infection. Taken together, these results demonstrate that Lon-1 is required by B. burgdorferi to infect animal hosts and to cope with environmental stresses.
V. Muigg et al.
Marcinkiewicz, A. L., Lieknina, I., Yang, X., Lederman, P. L., Hart, T. M., Yates, J., Chen, W.-H., Bottazzi, M. E., Mantis, N. J., Kraiczy, P., Pal, U., Tars, K., Lin, Y.-P.
The spirochete Borrelia burgdorferi sensu lato is the causative agent of Lyme disease (LD). The spirochetes produce the CspZ protein that binds to a complement regulator, factor H (FH). Such binding downregulates activation of host complement to facilitate spirochete evasion of complement killing. However, vaccination with CspZ does not protect LD infection. In this study, we demonstrated that immunization with CspZ-YA, a CspZ mutant protein with no FH-binding activity, protected mice from infection by several spirochete genotypes introduced via tick feeding. We found that the sera from CspZ-YA-vaccinated mice more efficiently eliminated spirochetes and blocked CspZ FH-binding activity than sera from CspZ-immunized mice. We also found vaccination with CspZ, but not CspZ-YA, triggered the production of anti-FH antibodies, justifying CspZ-YA as a LD vaccine candidate. The mechanistic and efficacy information derived from this study provides insights into the development of a CspZ-based LD vaccine.
Ruiyan Zhang, Ting Gong, Teng Chen, Ning Zhang, Faming Miao, Qi Chen, Ye Feng, Lu Ji, Jinghui Zhao
We read with interest the recently published article titled "Seroprevalence of Lyme borreliosis in Finland 50 years ago" [1].We applaud the authors for studyingthe Lyme borreliosis (LB) seroprevalence in Finland in the 1960s and 1970s through evaluation of IgG against Borrelia burgdorferiin historical serum samples. Despite its scientific and comprehensive, some technical issues should be discussed.
Strle K, Strle F.
Lyme borreliosisoutcomeposttreatment Lyme disease symptomsBorrelia burgdorferi sensu latobiomarkers
Quentin Bernard, Antoine Grillon, Cédric Lenormand, Laurence Ehret-Sabatier, Nathalie Boulanger
The skin plays a key role in vector-borne diseases because it is the site where the arthropod coinoculates pathogens and its saliva. Lyme borreliosis, particularly well investigated in this context, is a multisystemic infectious disease caused by Borrelia burgdorferi sensu lato and transmitted by the hard tick Ixodes. Numerous in vitro studies were conducted to better understand the role of specific skin cells and tick saliva in host defense, vector feeding, and pathogen transmission. The skin was also evidenced in various animal models as the site of bacterial multiplication and persistence.
Cuellar, J., Astrand, M., Elovaara, H., Pietikäinen, A., Siren, S., Liljeblad, A., Guedez, G., Salminen, T. A., Hytönen, J.
Borrelia burgdorferi sensu lato (sl), the causative agent of the tick-borne Lyme borreliosis (LB), has a limited metabolic capacity and needs to acquire nutrients, such as amino acids, fatty acids, and nucleic acids, from the host environment. Using X-ray crystallography, liquid chromatography-mass spectrometry, microscale thermophoresis, and cellular localization studies, we show that Basic membrane protein D (BmpD) is a periplasmic substrate-binding protein of an ABC transporter system binding to purine nucleosides. Nucleosides are essential for bacterial survival in the host organism and these studies suggest a key role for BmpD in the purine salvage pathway of B. burgdorferi sl. As B. burgdorferi sl lacks the enzymes required for de novo purine synthesis, BmpD may play a vital role in ensuring access to the purines needed for sustaining an infection in the host. Further, we show that although human LB patients develop anti-BmpD antibodies, immunization of mice with BmpD does not confer protection against B. burgdorferi sl infection.
Nayak, A., Schüler, W., Seidel, S., Gomez, I., Meinke, A., Comstedt, P., Lundberg, U.
The development of vaccines for prevention of diseases caused by pathogenic species can encounter major obstacles if high sequence diversity is observed between individual strains. Therefore, the development might be restricted either to conserved antigens, which often are rare, or to multivalent vaccines, which renders the production more costly and cumbersome. In light of this complexity, we applied a structure-based surface shaping approach for the development of a Lyme borreliosis (LB) vaccine suitable for the USA and Europe. The surface of the C-terminal fragment of outer surface protein A (OspA) was divided into distinct regions mainly based on binding sites of monoclonal antibodies. In order to target the six clinically most relevant OspA serotypes (ST) in a single protein, exposed amino acids of the individual regions were exchanged to corresponding amino acids of a chosen OspA serotype. Six chimeric proteins were constructed and based on their immunogenicity four of these chimeras were tested in mouse challenge models. Significant protection could be demonstrated for all four proteins following challenge with infected ticks (OspA ST1, OspA ST2, and OspA ST4), as well as with in vitro grown spirochetes (OspA ST1 and OspA ST5). Two of the chimeric proteins were linked to form a fusion protein, which provided significant protection against in vitro grown spirochetes (OspA ST1) and infected ticks (OspA ST2). This article presents the proof-of-concept study for a multivalent OspA vaccine targeting a wide range of pathogenic LB Borrelia species with a single recombinant antigen for prevention of Lyme borreliosis.
Célia Scherelle Boumbanda Koyo, Sandrine Lydie Oyegue-Liabagui, Oleg Mediannikov, Sébastien Cortaredona, Lady Charlene Kouna, Didier Raoult, Jean Bernard Lekana-Douki and Florence Fenollar
The epidemiology of febrile illness etiologies is under-explored in resource-poor settings. Establishing a local repertory of microorganisms circulating in blood of febrile and afebrile people is important for physicians. Blood was collected from 428 febrile and 88 afebrile children in Makokou (Gabon) and analyzed using polymerase chain reaction. Plasmodium spp. were the pathogens, which were most detected in febrile children (69.6%; 298/428) and in afebrile children (31.8%; 28/88) (P < 0.0001). Plasmodium falciparum was the most prevalent species in both febrile and afebrile children (66.8% and 27.3%, respectively). No differences were observed between febrile and afebrile children for Plasmodium malariae and Plasmodium ovale (8.2% versus 10.2% and 3.3% versus 3.4%, respectively). Triple infection with P. falciparum, P. malariae, and P. ovale was also detected in 1% of febrile children (4/428). Filariasis due to Mansonella perstans was detected in 10 febrile patients (2.3%), whereas Loa loa was detected in both febrile and afebrile children (1.4% and 2.3%, respectively). Bacterial DNA was detected in only 4.4% (19/428) of febrile children, including 13 (68.4%) who were coinfected with at least one Plasmodium species. These were Haemophilus influenzae (1.6%, 7/428), Streptococcus pneumoniae and Staphylococcus aureus (1.2%, 5/428), and Rickettsia felis (0.9%, 4/428). Coxiella burnetii, Bartonella spp., Borrelia spp., Tropheryma whipplei, Anaplasma spp., Leptospira spp., Streptococcus pyogenes, and Salmonella spp. were not detected. This study also highlights the over-prescription and the overuse of antibiotics and antimalarials. Overall, malaria remains a major health problem in Makokou. Malaria control measures must be reconsidered in this region.
Ndeye Safietou Fall, Nafissatou Diagne, Oleg Mediannikov, Florence Fenollar, Philippe Parola, Cheikh Sokhna, Didier Raoult, Jean-Christophe Lagier
Kristin Boardman, Kyle Rosenke, David Safronetz, Heinz Feldmann and Tom G. Schwan
African multimammate rats, Mastomys natalensis, are widely distributed in sub-Saharan Africa and live in close association with humans. In West Africa, numerous field studies have shown these animals may be naturally infected with the relapsing fever spirochete Borrelia crocidurae, the primary cause of tick-borne relapsing fever in this region of the continent. However, naturally infected individual rats have never been examined over time; therefore, the true host competency of these rats for this spirochete is unknown. Therefore, using animals from an established laboratory colony of M. natalensis, rats were experimentally infected with B. crocidurae and their blood examined to 28 days postinoculation. These animals were highly susceptible to infection and displayed prolonged and cyclic spirochetemias. Our results demonstrate these peridomestic rodents are likely competent hosts for infecting argasid tick vectors and play a primary role in the enzootic cycle for B. crocidurae in West Africa.
Bista S, Singh P, Bernard Q, et al.
AbstractBorrelia burgdorferi conserved gene-products BB0406 and BB0405, members of a common B. burgdorferi paralogous gene family, share 59% similarity. While both gene-products can function as potential porins, only BB0405 is essential for infection. Here we show that, despite sequence homology and co-expression from the same operon, both proteins differ in their membrane localization attributes, antibody accessibility, and immunogenicity in mice. BB0406 is required for spirochete survival in mammalian hosts, particularly for the disseminated infection in distant organs. We identified that BB0406 interacts with laminin, one of the major constituents of the vascular basement membrane, and facilitates spirochete transmigration across host endothelial cell barriers. A better understanding of how B. burgdorferi transmigrates through dermal and tissue vascular barriers and establishes disseminated infections will contribute to the development of novel therapeutics to combat early infection.
Gomes-Solecki M, Arnaboldi P, Backenson P, et al.
AbstractLyme disease, caused by some Borrelia burgdorferi sensu lato, is the most common tick-borne illness in the Northern Hemisphere and the number of cases, and geographic spread, continue to grow. Previously identified B. burgdorferi proteins, lipid immunogens, and live mutants lead the design of canonical vaccines aimed at disrupting infection in the host. Discovery of the mechanism of action of the first vaccine catalyzed the development of new strategies to control Lyme disease that bypassed direct vaccination of the human host. Thus, novel prevention concepts center on proteins produced by B. burgdorferi during tick transit and on tick proteins that mediate feeding and pathogen transmission. A burgeoning area of research is tick immunity as it can unlock mechanistic pathways that could be targeted for disruption. Studies that shed light on the mammalian immune pathways engaged during tick-transmitted B. burgdorferi infection would further development of vaccination strategies against Lyme disease.
The aim of the study is to assess anti-Coxiella burnetii antibodies presence in inhabitants of north-eastern Poland, to assess the risk of Q fever after tick bite and to assess the percentage of co-infection with other pathogens.
The serological study included 164 foresters and farmers with a history of tick bite. The molecular study included 540 patients, hospitalized because of various symptoms after tick bite. The control group consisted of 20 honorary blood donors. Anti-Coxiella burnetii antibodies titers were determined by Coxiella burnetii (Q fever) Phase 1 IgG ELISA (DRG International Inc. USA). PCR was performed to detect DNA of C. burnetii, Borrelia burgdorferi and Anaplasma phagocytophilum.
Anti-C. burnetii IgG was detected in six foresters (7.3%). All foresters with the anti-C. burnetii IgG presence were positive toward anti-B. burgdorferi IgG and anti-TBE (tick-borne encephalitis). Anti-C. burnetii IgG was detected in five farmers (6%). Four farmers with anti-C. burnetii IgG presence were positive toward anti-B. burgdorferi IgG and two with anti-TBE. Among them one was co-infected with B. burgdorferi and TBEV. Correlations between anti-C. burnetii IgG and anti-B. burgdorferi IgG presence and between anti-C. burnetii IgG presence and symptoms of Lyme disease were observed. C. burnetii DNA was not detected in any of the 540 (0%) patients.
C. burnetii is rarely transmitted by ticks, but we proved that it is present in the environment, so it may be a danger to humans. The most common co-occurrence after tick bite concerns C. burnetii and B. burgdorferi.
A. J. Henningsson et al.
Lyme borreliosis (LB) is a tick-borne disease caused by spirochetes belonging to the Borrelia burgdorferi sensu lato species. Due to a variety of clinical manifestations, diagnosing LB can be challenging, and laboratory work-up is usually required in case of disseminated LB. However, the current standard of diagnostics is serology, which comes with several shortcomings. Antibody formation may be absent in the early phase of the disease, and once IgG-seroconversion has occurred, it can be difficult to distinguish between a past (cured or self-cleared) LB and an active infection. It has been postulated that novel cellular tests for LB may have both higher sensitivity earlier in the course of the disease, and may be able to discriminate between a past and active infection.
VICTORY is a prospective two-gate case-control study. We strive to include 150 patients who meet the European case definitions for either localized or disseminated LB. In addition, we aim to include 225 healthy controls without current LB and 60 controls with potentially cross-reactive conditions. We will perform four different cellular tests in all of these participants, which will allow us to determine sensitivity and specificity. In LB patients, we will repeat cellular tests at 6 weeks and 12 weeks after start of antibiotic treatment to assess the usefulness as 'test-of-cure'. Furthermore, we will investigate the performance of the different cellular tests in a cohort of patients with persistent symptoms attributed to LB.
This article describes the background and design of the VICTORY study protocol. The findings of our study will help to better appreciate the utility of cellular tests in the diagnosis of Lyme borreliosis.
Trial registration
NL7732 (Netherlands Trial Register, trialregister.nl).
International Liver Congress (ILC) 2020
London, England
15.04.2020 - 19.04.2020
European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 2020
Paris, Frankrig
18.04.2020 - 21.04.2020
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Google develops AI that can predict your risk of heart disease by scanning your eyes
Here's yet another potential breakthrough in the field of health care and Google is responsible for it. The California-based company has developed an AI system that can scan your retinas to assess your risk of heart disease has been developed. WhileRead More


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Baby Got Bach VR Porn Every Tuesday afternoon you secretly look forward to the forty five minutes you get to spend with Alex Blake. After all, you're her private piano teacher. You know she's only eighteen but you still can't help getting turned on by read more
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The Repair Man VR Porn Your life as a repairman is pretty boring…until today! Petite Latina Holly Hendrix comes outside to greet you at the front door. She's looking all coy and cute, just suckin' on her lollipop. She's talking to her dad on the read more
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Component manufacturers - Manufacturer
35 rue Thomas Edison
33610 - CANEJAN - France
Phone: +33 5 35 54 49 59
Sector of intervention
Under sector of intervention
BASE is a French SME specialised in solar energy, founded in 2009. BASE has developed Cogen'Air, the rst Thermovoltaic solar panel producing electricity and heat.
The Cogen'Air solar panel produces 1000W of energy (250W of electricity and 750W thermal), to reduce buildings and industrial drying processes energy expenditure and generate photovoltaic revenue. Cogen'Air increases the value of dried materials (forage, wood, agri- food) and reduces their transportation costs (waste).
BASE carries out energy studies for companies wishing to reduce their operating costs, reduce their environmental impact and increase their energy independence. These studies are a decision-making aid in order to efficiently and durably reduce their consumption and their carbon footprint by improving the electrical and / or thermal efficiency of their buildings / processes.
BASE has several references in France for solar drying (forage, wood, waste) and for energy efficiency of buildings (electricity, heat, hot water production...). BASE has partners in Morocco and Brazil and is seeking other partnerships to increase international development.
Key figuresStaff: 15
Turnover: --
International turnover: --
ContactMs Stephanie Ackermann
Responsable Administrative
Phone: +33 5 35 54 49 59
Email: firstname.lastname@example.com
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I just finished watching CNN Journalist Anderson Cooper's interview with the sister of detained American Kennith Bae. As usual, Mr. Cooper and Dom Lemon Before him followed company orders and wasted no time vilifying Dennis Rodman because he is not using his position as a friend of the Supreme Leader of North Korea to advocate for Bae's release. They intentionally misquoted him with the reairing of the full interview to try to say Rodman doesn't give a rat's ass about Mr. Bae's situation. This despite the fact Rodman himself made it clear he is not
- a Diplomat
- a politician
- going to get into North Korean/U.S. Relations
The "rant and tirade" CNN called it? He was understandably furious. Yes, his genuine friendship with Kim Jong Un is mutual. Yes, he knows about Mr. Bae's situation. Yes, he knows very cleary about what's going on in North Korea. If you look carefully at the video footage of Rodman in Pyongyang you will notice something you don't see when reporters visit North Korea: No government minders are trailing him. CNN Journalist Wolf Blitzer was pretty adamant about government minders following outsiders from the West everywhere.
OK, look: Try to look at this from Rodman's perspective. Like he said when the reporter in America who was interviewing him tried to bait him into talking politics, he and the players with him are IN North Korea right now. He's not stupid. The players with him aren't stupid. They're not going to say ANYTHING that might jeopardize their safety or their freedom to leave the country. What the reporter who did the interview tried to do–and Rodman drove the point home very clearly–was bait him into a subject that could put them in danger.
Remember: Both the U.S. Justice Department and the NBA have denounced the players who've chosen to participate in this cultural exchange, which doubles as a birthday present for Kim Jong Un. What the West keeps ignoring is if this cultural exchange is a success, it will lead to other things. It's obvious Kim and Dennis can directly speak to each other without a translator so…yeah. It's also seems North Korea isn't opposed to the idea of opening relations with "the enemy". Otherwise why invite Rodman back into the country?
All these North Korean refugees jumping down Rodman's throat on Facebook KNOW he is in an influential position. If he speaks to The Supreme Leader, he will be heard. Going back to Rodman's thoughts on Mr. Bae's situation, he said something interesting before he stopped himself in that interview this morning: "You don't know what happened." It's not what he said but the way he said it that I found most telling. Watch the interview again. Notice the increased emotion when he says that sentence. I think Dennis Rodman does know why Mr. Bae is still being held by North Korea. For reasons we don't know or understand he's not going to talk about the why. There's also the fact we know Mr. Bae is still alive and is in a hospital in Pyongyang. If they wanted him dead he would've been killed already but he's being taken care of. He is in a hospital room, not a jail cell.
I believe Dennis Rodman will one day be vindicated as a diplomat in the eyes of the west. As I said on Facebook earlier tonight this game could lead to other things down the road.


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Search Results:
Enter your search terms below. You can choose to filter your results by section by choosing from the drop-down menu below. Also, in some sections you can choose to filter by system as well!
Example: To search for Pac-Man in MAME ROMs type in "pac-man", choose "ROMs/ISOs/Games" as your Section and "M.A.M.E." as your system. It's just that simple!
You searched for "running high" in ROMs/ISOs/Games:
2 Results FoundSwitch between ROMs, Emulators, Music, Scans, etc. by selecting the category tabs below!Save time. Add Emuparadise search to your browser search bar!
Running High (Japan) ISO
System: Sony Playstation Size: 464M
System: Sony Playstation Size: 464M
Digimon World 2 [NTSC-U]*
Playstation One Links (EPForums Registration Required)
Playstation One Links (EPForums Registration Required)


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Hey Neighbor, I want to meet your neighborhood!
"Too often we expect our citizens to come to city government. However, I understand that people have busy lives, and are not always able to come to public meetings or other community events to voice their concerns. As a result, we are shifting our approach and having city government come to you. My hope is to learn about what is going on in your neighborhood and discuss critical issues impacting our community," said Mayor Mike Nelson.
These meetings are intended to provide the community with an opportunity to ask questions, openly discuss how to make the Edmonds community better, and to engage in conversations with me about important issues in your neighborhood.
If you'd like to schedule a meeting in your neighborhood please contact us at 425.771.0247 or email@example.com
Kristiana Johnson Council Position #1
Contact Phone: 425.533.6105 • email: firstname.lastname@example.com
Term Expires: 12/31/2021
2020 COUNCIL APPOINTMENTS: 1) Public Safety and Personnel Committee 2) Historic Preservaton Advisory Commission 3) Lodging tax Advisory Committee 4) SNOCOM 911
OPEN -- Council Position #2
Contact Phone: • email:
Term Expires: 12/31/2021
Adrienne Fraley-Monillas Council Position #3
Contact Phone: 206.890-4562 email: firstname.lastname@example.com
Term Expires: 12/31/21
2020 COUNCIL APPOINTMENTS: 1) Finance Committee 2) Affordable Housing Alliance (alt) 3) Diversity Commission 4) PFD Oversight Committee 5) Snohomish Health District
Diane Buckshnis Council Position #4
Contact Phone: 206.228.3462 • email: firstname.lastname@example.com
Term Expires: 12/31/2023
2020 COUNCIL APPOINTMENTS: 1) Parks, Planning and Public Works Committee 2) Lake Ballinger Work Group 3) SeaShore Transportation Forum 4) Salmon Recovery - WRIA 8 5) Tree Board
Vivian Olson Council Position #5
Contact Phone: 425.361.8176 • email: email@example.com
Term Expires: 12/31/23
2020 COUNCIL APPOINTMENTS: 1) Finance Committee 2) Disability Board 3) Economic Development Commission 4) Housing Commission
Susan Paine Council Position #6
Contact Phone: 206.361.8844 • email: email@example.com
Term Expires: 12/31/23
2020 COUNCIL APPOINTMENTS: 1) Parks, Planning and Public Works Committee 2) Disability Board 3) Port of Edmonds 4) Salmon Recovery - WRIA 8 (alt)
Laura Johnson Council Position #7
Contact Phone: 425.361.8229 • email: email@example.com
Term Expires: 12/31/23
2020 COUNCIL APPOINTMENTS: 1) Public Safety and Personnel Committee 2) Community Transit 3) Mayor's Climate Protection Committee 4) Youth Commission
If you have questions, topics you'd like to hear about, or general feedback, please send us an email to Update on Edmonds


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Recent trends in pre-marital fertility across sub-Saharan Africa
The rising age of first marriage across sub-Saharan Africa over the past 25 years has led many scholars to predict a concurrent increase in pre-marital childbearing. We examine whether this anticipated increase has materialized using data from 27 countries. Our results reveal considerable heterogeneity. Although levels of pre-marital fertility have risen by up to 13% in recent years in some African countries, other countries have experienced a 7% decline.


text:
Diamonds and Dogs
This is reality, and GW Pharmaceuticals (GWPH) is rising. Today they announced their Cannabidol (CBD) placebo test on Schizophrenia to have top line results, causing shares to be up 7% today. The patients that received the CBD showed a significant improvement on three different scales. Although this was an exploratory study, expect more to come from them in the future. GW's CEO plans on exploring more on the effects of CBD for multiple medical problems.
Enjoy your stay at LaSalle's (LHO) hotels. You might get comfortable, luxury hotels that LaSalle renovated, but their share price today dropped 4%. The company missed expectations of $345.1 million by $3.7 million. For the future they plan to rebrand three of their hotels in San Francisco that they hope will increase performance in their next quarter. They also reported a $.45 dividend per share today as well. With the holiday seasons right around the corner expect LaSalle to do better.


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Blueprints Requiring Neurodes
MarketNeurodes can be purchased directly from the market for 10. Alternatively, players can purchase their blueprints for 100, or doing Raid The Law of Retribution Nightmare where the permanent blueprint can be dropped as a mission reward, blueprints are permanent and reusable. (Edit: The Law of Retribution Nightmare raid is no longer available for play until an undisclosed date when Raids have been reworked and re-released).
|Manufacturing Requirements
|Time: 1 hrs
|Rush: 2
|Market Price: 10
|Blueprints Price: 100
Gathering Tips
These are based on opinions and may not be true. These should be viewed as advice unless proven undoubtedly true. Check comments for confirmation. Results may vary. Please note each of the tips below is a subjective average, and may not necessarily represent your experiences.
- Plato (Lua) guarantees a spawn of Sentients within the first couple of minutes. This is the most effective way of farming Neurodes, but bring a party, as Sentients do prove difficult.
- Orokin Derelict Assassinations (Lephantis) are one of the easiest ways to acquire Neurodes (1-2 average every run), although Derelict Assassination Keys can only be created once every hour. These special derelict keys also require Lephantis Nav Coordinates, which can only be acquired through other Orokin Derelict Missions.
- Newer players might find it helpful to search missions in Earth for Neuroptic Masses, the resource container for Neurodes. The chance for enemies to drop Neurodes is very low, making it worth spending time to search for the containers instead.
- Sentient fighters spawning on Lua have a relatively high chance of dropping Neurodes (can be accessed upon completing The Second Dream quest).
- A widely held misconception among players is that Neurodes are easy to acquire on Orokin Derelict Defense Missions. Not only can this be difficult for some players, but it averages 1 Neurodes every 30 waves. (tested with a Nekros performing Desecrate, may need further testing to confirm).
- Another misconception is Orokin Derelict Survival, which does yield more than the defense mission. However it is about 3-4 Neurodes for every 30 minutes, assuming that you have a Nekros performing Desecrate.
- Eris survivals and defense (including Dark Sectors) do NOT yield many Neurodes, about 1 for every 20 minutes/waves.
- The singular form of Neurodes is Neurodes, according to the game.
- The descriptions of Neural Sensors and Neurodes were swapped several times, at one point completely reversing what the components clearly were. This was finally reverted in Hotfix 15.16.2 to properly fit their obvious designs.
- In computing, Neurodes (likely a portmanteau of neuron and node) serve as the building blocks of artificial neural networks.
Community content is available under CC-BY-SA unless otherwise noted.


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Scientific proof if your partner is not being faithful
The question of do I have a cheating spouse is unfortunately a normal question now to ask in most marriages. Sexual infidelity is a common issue in many relationships and unfaithfulness is the number one reason for divorce and breakups world wide. In many studies it is conservatively approximated that over 60% of husbands and 40% of wives will cheat at some point during the course of their marriage.
It is a sensitive subject that often is not easy to find "evidence" to prove. In your gut you feel like you know the truth but then how can you be sure it is not just imagination or paranoia? Well infidelity is no longer a guessing game. DNA testing can provide a conclusive way to find out if you have a cheating spouse or not.
Have you discovered suspicious items such as unexplainable stains on clothing or sheets or other items?
With infidelity DNA testing the items you send us such as underwear, condoms, bed sheet swatches, etc. that have suspected fluids on them can be tested to determine if DNA from a male or female or both was on the suspicious items. If the items tested are confirmed to contain DNA we can then perform a comparative analysis to identify whose DNA it is.
At DNA Testing Experts we know how emotionally devastating suspecting having a cheating spouse can be and how it can destroy relationships and even entire families so we handle each case with care, discretion and compassion and provide the most accurate and conclusive results possible.
Considering the life changing consequences of these answers to your suspicions make sure you are getting the most accurate testing possible. It would be tragic to break up a relationship or stay when you should leave over a false result. The cheap self test kits you can buy online will simply test for substances that glow under a UV light but that doesn't necessarily mean if it glows it is semen. Blood and urine also glow under a UV light. These tests risk giving you a false positive result that will cause more harm than good. The DNA testing we do is discreet, accurate and can give you the answers you need to know quickly.
Sources of DNA That Can be Used for Cheating Spouse Infidelity Testing
During the course of sex and even for days afterward, semen and vaginal fluids can be transferred to undergarments, panties, feminine products, clothes, sheets and a variety of other types of items. Semen can also stay in the vagina for up to five days after sex; and can still be detected for an even longer period of time when it becomes a dried stain.
The types of samples you can send us vary widely and the chances of us being able to successfully extract DNA profiles depends on the samples you send in to us. Often items can contain enough DNA for analysis but there may be exceptions. In some cases the amount of DNA in the sample may just be too small for any significant analysis; other issues could be the DNA sample provided is contaminated with chemicals or mold (if it was stored in a plastic bag) which might corrupt or degrade the DNA for testing or the DNA may just be too old.
How the Infidelity DNA Testing Works
The DNA detection and comparison process has several steps to it. The first step is sending you a DNA collection kit. You would then collect a mouth swab of your DNA or the DNA of the person you wish to test the suspected items against. This swab is the control sample. After you have collected your sample you would then send it to our lab for testing. Alternative types of samples may be sent in other than a cheek swab such as hair with the roots attached, toothbrushes, nail clippings, cigarette butts, chewing gum, etc. Please see our Alternative Samples page for more information on this.
Infidelity DNA testing processes the item or garment you send in to detect the presence of male and/or female DNA. In the lab, if DNA is detected on the sample during the DNA detection process, then a DNA profile will be compiled. This DNA is then identified as male or female DNA and may even have multiple DNA profiles found on the tested item. You have the option to compare the DNA found on the item against your DNA control sample. This is often done to rule yourself out as a donor.
PLEASE NOTE: Any articles sent in will not be returned.
How to Collect an Infidelity DNA Testing Sample
- The preferred infidelity DNA testing sample is a piece of clothing that you suspect has semen on it. We realize in some instances that it might be difficult to submit the actual test item for fear that you will be discovered. Clothing items are not the only option.
- Another infidelity test DNA sample choice could possibly be a dried stain from clothes or bed sheets or even a vibrator. This should be done using a sterilized razor blade or knife. Sterilization should be done with running the blade through a flame or boiling water as opposed to alcohol or bleach as the chemicals might degrade the DNA sample. Carefully scrape the dried stain substance into a clean paper envelope.
- Another possible method for collecting an infidelity DNA test sample is by using lightly moistened Q-Tip swabs. Using chlorine free filtered, or preferably distilled water roll the lightly moistened Q-Tip firmly a few times on the suspected substance you are wanting to test. Let the Q-Tips to air dry for about an hour. After the Q-Tips have sufficiently dried place them in a clean paper envelope.
cheating spouse
The infidelity DNA test results will be conclusive, 100% accurate and you will finally know the truth about whether you have a cheating spouse or not.


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opticalRendu for Roon
opticalRendu for Roon
The Sonore opticalRendu is a Roon Ready network player and network streamer. The opticalRendu turns your USB DAC into a network DAC.
You can play to your DAC from a computer or a music server such as or sonicTransporter. All playback can be controlled via an iPad, tablet or computer.
The opticalRendu uses small ultra low noise regulators for each component and FEMTO oscillators to get the absolutely cleanest signal to your DAC. This manifests itself as very clean sound with revealing detail.
The opticalRendu network player and network streamer utilizes a fiber optic interface to eliminate all noise from the network. This eliminates the need for expensive copper Ethernet cables.
The only difference between the opticalRendu for Roon and our standard opticalRendu is the opticalRendu for Roon only run the Roon Ready player and the standard opticalRendu run many different software player types.
If you only use Roon the opticalRendu for Roon is the player/streamer for you.
- Supports the following PCM sample rates up to: 44.1kHz, 48kHz, 88.2kHz, 96kHz, 176.4kHz, 192kHz, 352.8kHz, 384kHz, 705.6kHz, and 768kHz
- Supports the following DSD sample rates up to: DSD64, DSD128, DSD256, DSD512
- POP free DSD/PCM transitions
- Not all USB devices or output modes support the maximum sample rate of the unit
- RoonReady certified
- Supports Tidal and Qobuz lossless streaming through Roon
- Native DSD is supported on select DACs
- The unit is controlled via the Roon app mobile device or on your computer
- Based on Sonicorbiter, closed source and open source (GPLv2)
- High-quality ultra low noise regulators
- External power input jack
- SFP cage / SFP optical module for network connection (OM1 SFP Transceiver included)
- USB-A audio output connector
- Micro SD port for the operating system
- Two fixed frequency ultra low jitter FEMTO oscillators
- Multicore processor with DDR3 RAM
- Power input: 6-9 VDC at 2.2 Amp min continuous
- Power connector: 2.1mm x 5.5mm (center +)
- Dimension without rubber feet: 1.18"(H) x 4.33"(W) x 4.41"(D)
This product includes:
- Sonore opticalRendu for Roon
- SFP module
Note: Although many different types of fiber and SFP transceivers will work we recommend Gigabit SFP modules that work with OM1 (62.5/125 µm multi-mode) type multi-mode fiber with LC connectors for all systemOptique certified products. This will allow your systemOptique to work at its highest level of performance. Don't worry, we supply these SFP transceivers on our website
- Small Green Computer Linear Power Supply - 7VDC - recommended
- CIAudio linear power supply - 7VDC
- Teddy Pardo linear power supply - 7VDC
- HD-Plex Linear power supply - adjust to 7VDC
- Sbooster linear power supply - 6VDC
- Uptone Audio LPS-1/LPS-1.2 power supplies - not recommended
We Also Recommend


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Bandol Rose Chateau Val d'Arenc
Laudable Rosé reminding us how great rosés can be. Impeccable structure and finesse. Lean and concentrated with freshness and red fruit in good measure. Juicy, intense and a perfect thirst quencher. Equally a stand up wine to strong fish dishes and charcuterie.
Origin: Bandol, France
Vintage: 2017
Grape variety: Mourvédre, Cinsault and Grenache
Food pairing: Fish stew, crab terrine
Bottle size: 75cl
London E17 postcodes: Free
Available for deliveries within London E17 postcode only. We deliver on Wednesdays & Saturdays (6pm to 9pm)
Click & Collect: Free
Place your order online, then pop by to our shop (151 Forest Rd, E17) during our opening hours to pick up your bottles. We will contact you as soon as your order is ready.
Standard Delivery: £8.95
Place your order online and get delivered to your home address within 5 working days
Free to store
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REOPro - Real Estate Default Professionals
America's Largest Social Network for Default Real Estate Professionals
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I currently have Ala Mode hosting my website but have been thinking about changing. It is very user friendly and easy to change BUT I don't seem to be getting much traffic or leads. Does anybody…Continue
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I am trying to grow my REO business and thought going to one of the conferences would be one of the best ways. Does anybody have any suggestions which would be the best conference to attend? I know…Continue
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