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45Nrth's Wölvhammer uses NASA-grade insulation to keep your feet warm when temperatures plummet below freezing
Punishing terrain, brutal conditions, and no guarantee of finishing
When winter drives you indoors, stationary trainers can keep your wheels spinning. Here's how the CycleOps Fluid2 simulates riding.
From the outside, internally geared hubs seem simple, but complicated works are inside
What's cool at Interbike? Heat dissipating carbon wheels
Big brands sell bikes straight to consumers
Disc brakes continue their spread while fat bikes see more innovation
Japanese component maker set to offer hydraulic disc brakes for road and cyclocross
Industry gathers in desert with bigger, fatter tires to sweat
Revolutionary new gear can change the Tour forever
Company becomes first bike brand to control its own aero testing facility
An expanded high-end range can boost your speed, whether the roads are dry or wet
The Globe Roll 8 Rare is stylish, fun, practical, and as hard to get as its name implies
High-tech sole has flex and traction for walking but still pedals fast
Titanium bikes aren't popular or cutting-edge—but their ride is unique and absolutely astounding
Italian footwear brand introduces new road and mountain models
Two carbon models use Avid calipers
Bura SL ready to compete on American roads
This Swiss E-bike is ready to speed you around town
British clothing company releases its first road shoe
New gear includes Di2 for city bikes and disc brakes for road and CX
A tribute to the life and death of Patrick Ytsma
A Giro Stage 6 winner. We review Campagnolo's long-awaited EPS shifting group


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Roxy-Pacific snaps up two sites in Australia for A$67.4m
PROPERTY firm Roxy-Pacific Holdings on Tuesday said it plans to buy two vacant sites in Glebe, Sydney, Australia for A$67.4 million (S$68.9 million).
These are two city fringe residential development sites comprising a total land area of about 7,125 square metres, with a development potential of 248 residential units.
The acquisition will be financed by internal funds and bank borrowings.


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Frequently Asked Questions
If you and I were sitting face-to-face, chatting in your office perhaps, you'd look me in the eye and ask me questions.
Let me try to answer a few of those questions right here and now…
What are your qualifications as a copywriter?
For a while copywriting has been described as salesmanship in print. However, I prefer the definition by arguably the greatest living copywriter - Gary Bencivenga. He describes copywriting as salesmanship multiplied and leveraged onto different media and delivery systems.
That being said, I had my start selling advertising to businesses in the London area while in college. Then I sold tradeshow spots and business services for a stint.
After college, I decided the London property market was my quickest way to the charmed life I desired... So, I weighed my options, decided on a property company and got into the property business. For over 6 years I sold, resold, bought, rented, and had myself knee deep in the London property business.
It was while I was in property business I discovered the power of the written word.
I noticed a few of my property descriptions attracted tons more phone calls and viewings; while others got flimsy enquiries and little to no viewing activity.
So, in my bid to make sure all my property listings were buzzing with lots of enquiries and viewings, I stumbled upon a copywriting training put together by some of the 'Who is Who' in the direct response copywriting business… At the time, I didn't know much about the creators - Bill Bonner (Agora), Michael Masterson, and Paul Hollingshed, but I went ahead and ordered the course.
I burned the midnight candle and studied the course non-stop until I completed it. Then I started implement what I learned to my property descriptions, brochures, and canvassing letters.
Soon, all my listings were getting a lot of attention - enquiries and viewings improved tremendously, and my sales were no.1 in the company. The last time I checked; the canvassing letters I wrote while working there is still being used in the company till this day.
My new found copywriting skills put me in good stead. When the recession hit in 2007, many businesses were struggling, while others just shut their doors. Regardless of the devastation going on around me, I was still able to make 2007 and 2008 my best years in sales and income. And I attribute much of that success to my ability to write compelling sales and marketing copy.
And meanwhile, in my spare time I was also using my copywriting skills to help businesses – around the UK, US, and Canada – sell their goods and services online for a slice of the profits.
By October 2009 the demand for my services got to a point I had to send my boss my most cherished letter yet. So, I resigned and went into my own business fulltime.
Do you have experience in the alternative health field?
1 of the main (entirely justified) complaints of alternative health clients is that copywriters deliver over the top, hypey sales copy that can't possibly be approved by compliance. Because the copy would most definitely put the client in some legal hot water if they put it out to the market. What good is copy you cannot use?
I'm not a Chiro, hypnotherapist or naturopath who turned into a copywriter. I've spent most of my adult life in sales and marketing: belly to belly, toe to toe, and face-to-face selling to real human beings, overflowing with desires, emotions, and fears for the future. People just like me.
So, what makes me uniquely equipped to write copy and create marketing for alternative health clients?
It's not just my ability to write persuasive copy, or my market savvy…
The fact is, I'm an alternative health buyer. I'm a dyed in wool believer in alternative health remedies.
I invest my hard earned money in alternative therapies - from natural supplements, super foods, fitness training DVD, and physical therapy (like acupuncture massages) just to name a few.
No one knows your market, like your market.
And I already am the ideal prospect you want to market to.
What kinds of assignments/projects do you handle?
I cover the full range of copywriting services you will need to create a supercharged, high converting, sales generating marketing funnel and web presence.
About 80% of my business is helping alternative health companies connect, compel and communicate with the market in ways that tug heartstrings and open wallets.
I do this using a varied assortment of marketing communications, including ad copy, video sales letters (VSLs), advertorials, emails, articles/blog posts, newsletters, landing pages, webpages, white papers, and SEO web content.
My specialty is my ability to create clear, credible, persuasive copy interwoven with unique stories that are embedded with powerful psychological triggers which hypnotically compel your audience to take action.
I am able to unearth the "cool" and "sexy" in a somewhat boring product, so your prospects continuously salivate to get their hands on it. Some of my clients also appreciate my ability to break down complex and complicated topics into simple metaphors that anyone can understand. And this makes it easy for me to pick apart complex topics or mechanisms, and explain it in ways even a 10 year old can grasp.
You never need to worry about your copy sounding to academic and boring when it comes to explaining the technical side of your product or service.
Do you offer consulting services?
In addition to writing copy, I also offer my services to a couple of clients as a consultant. I help them plan their campaigns - complete marketing funnel strategy, architecture, and creation. My clients are always thrilled to get targeted advice that works - at an affordable fee - with the bulk of my fee tied to performance.
Your fee schedule lists price for a 'Website Audit'. What's that?
As you would already know, having a beautiful looking website doesn't guarantee your success online. With a strategic website audit, my clients are provided an objective review of their website; this includes the homepage, about page, and all other complimentary pages. However, you may choose the specific pages you want me to audit. It's up to you.
When you order a website audit, you get a written report of 2 or more singlespaced typed pages, with relevant marked up screenshots which identify problem areas. I'll show you what's good about it, what works… What doesn't work… And what should be changed, to help you achieve your goals… And how. My audit covers your website copywriting, SEO, effective design, strategy, conversion optimisation, and your offer. It also includes specific directions for revisions and improvements, although I don't rewrite copy or implement the improvements for you under this arrangement.
A website audit is ideal for clients who want a 2nd opinion on a newly launched website, a grossly underperforming one, or if you need fresh ideas to inject into an existing site which you know could be performing better. An audit also enables you to sample my expertise at far less cost than you'd pay to have me write your entire website, or create a sales funnel from scratch.
Speaking of money. What does it cost to hire you for a project?
Frankly, I don't consider my marketing and copywriting services as a "cost" to my clients. I prefer to consider it an investment in your business. And as an investor in your business, you expect a return on every money invested. I am confident that every project or assignment we do together would give you a healthy return on your investment (ROI), or I'll rewrite it, tweak it, re-strategise it until it does.
Now we've clarified that… For any assignment/project; sales letter, an advert, advertorial, landing page, website, marketing funnel - just let me know what you have in mind, or what you want to achieve and I'll quote you a price. I have also enclosed an estimate of my fee schedule which gives typical prices for a variety of projects.
How long will it take you to write copy for me?
Ideally, I'd like to have 2 to 4 weeks to work on copywriting projects. This gives me the time to research, conceptualise, write, polish, edit, and revise until I am happy with every single word.
Nevertheless, I realise you can't always wait that long. So if you require an urgent job, just indicate that when you order, stating the date you need to receive your copy by. If I can accommodate that, I'll let you know. And if I take on the job, I guarantee that you will have the copy on your desk by the deadline, or sooner.
Regardless of the urgency or tight deadlines, the copy I submit to you will always receive my usual thorough and professional attention. You can count on that.
What happens if we want you to revise the copy?
If you desire any improvements or tweaks to my already delivered work, just let me know what you want improved and what the changes are, and I'll make them fast. They'll be no charge for rewriting. Revisions are included in the fee we agree for the assignment, as long as they're assigned within 30 days of you receiving my delivered work. And are not based on a change in the assignment, which was made after my completed work has been submitted.
Most clients are delighted and ecstatic about my work when they receive it. But if for some reason you're not 100% thrilled, I'll revise the copy according to your specific guidelines… all at my expense.


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Patrick Raddatz
Patrick is a researcher, artist, music facilitator, working on scientific and artistic research projects focussing on the evolution of House and Techno in Germany, as well as on obsolete media technologies and photography. He currently has a teaching assignment at the University of Art and Design Offenbach and is working at the Institut für Klangforschung and the independent art space saasfee*pavillon.
Having graduated in Visual Communications with majors in Experimental Spatial Concepts and Photography, but originating from an electronic music background as a dj and producer, he is regularly working on concepts for and realizing events such as ROTOR and Robert Johnson Theorie in cooperation with the University of Art and Design Offenbach, the Institut für Klangforschung and partnering institutions. Patrick's thesis «HOUSE NATION BRD – Regionale Netzwerke in House und Techno von den Anfängen in den 1980ern bis zur Definition eines städtetypischen Sounds in den 1990ern» has been featured at Robert Johnson Theorie and the B3 Biennale.


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Tuesday, 31 December 2013
Pastor Arrested Along With His Kids Was Involved In Fake Money Printing.
The Department of State Security Service, SSS, in Lokoja, Kogi on Tuesday announced the ARREST of a syndicate, including a pastor, for the alleged printing and circulation of fake naira notes. The Director of SSS, Mike Fubara, who presented the suspects to journalists, said the syndicate included 15 others, including the pastor's six children. He named the pastor as Godson Akubuiro, the General Overseer of The Mountain of Breakthrough Deliverance Ministry, Lagos.
Mr. Fubara said the SSS in its effort to trace the source of the "worrisome fake naira currency in circulation in the state," undertook a covert operation to uncover those behind the act. "This operation took the service close to four months before a breakthrough resulting in the arrest of the 16-man syndicate led by Reverend Godson O. Akubuiro," the director said. He said that the operation took security agents to The Breakthrough Church, also known as Land of Solution, located at Agric, Ikorodu, Lagos. He said that the suspects were being investigated preparatory to their prosecution. The director said items recovered from the suspects include equipment and materials used in printing fake currencies. Other items recovered were a large quantity of printed fake notes, cut to size blank currency notes and N1.3 million fake naira notes.
Mr. Fubara urged the public to be wary of the fake naira notes in circulation and report suspects to the service.
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"Billy" Butlin (29 September 1899 – 12 June 1980) was a British, South Africa-born entrepreneur whose name is synonymous with the British holiday camp. Although holiday camps such as Warner's existed in one form or another before Butlin opened his first in 1936, it was Butlin who turned holiday camps into a multimillion-pound industry and an important aspect of British culture.
Born in Cape Town, South Africa, to William and Bertha Butlin, Butlin had a turbulent childhood. His parents separated before he was seven, and he returned to England with his mother.
He spent the next five years following his grandmother's family fair around the country where his mother sold gingerbread; exposing the young Butlin to the skills of commerce and entertainment. When he was twelve his mother emigrated to Canada, leaving him in the care of his aunt for two years. Once settled in Toronto, his mother invited him to join her there.
In Canada Butlin struggled to fit in at school and soon left for a job in a Toronto department store Eaton's. In the First World War he enlisted as a bugler in the Canadian Army. After the war, Butlin returned to England, bringing only £5 with him. Investing £4 of that money to hire a stall travelling with his uncle's fair, Butlin discovered that giving his customers a better chance to win brought more custom in, and he quickly became successful. One stall became several, including prominent locations such as Olympia in London, and Butlin soon was able to purchase other fairground equipment, and started his own travelling fair. He proved successful in this endeavour as well, and by 1927 he opened a static fairground in Skegness. Over the next 10 years Butlin expanded his fairground empire, all the time harbouring an idea to increase the number of patrons in his Skegness site by providing accommodation.
Butlin's first holiday camp opened at Skegness in 1936, followed by Clacton, two years later. Plans to open a third in Filey were cut short by the outbreak of the Second World War. Butlin used the war to his advantage, persuading the MoD to complete the Filey Holiday Camp and construct two more camps in Ayr and Pwllheli as training camps which he reclaimed when the war was over. In the post-war boom, Butlin opened four more camps at Mosney, Bognor Regis, Minehead and Barry Island as well as buying hotels in Blackpool, Saltdean, and Cliftonville. By the time he retired in 1968, Butlin had allowed hundreds of thousands of holidaymakers a self-contained vacation with a variety of entertainment.


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Home »
Special Projects
Personal photography projects of Rob Greer including landscape photography, flora photography, and the Found Items Project.
Landscape PhotographyRob Greer is a nature photographer based in Houston who is best known for his amazing landscape photography of Yosemite National Park.
Yosemite National Park: Winter PhotographsThese pages feature a winter photo collection from Yosemite National Park. Many images were captured at great distances using a super-telephoto lens.
Flora PhotographyThese photographs of various succulents and plants captured by Rob Greer over a span of several years are provided simply for your viewing pleasure.
Found ItemsGallery of found items includes whimsical photographs and images intended to infer observations on society or simply be visually interesting.
Fine Art Nude PhotographyLike many photographers in Houston, Rob Greer has explored the fine art nude photography and has refined and developed a highly recognizable style.


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I have been practicing law since 1980. Throughout my career, lawyers have discussed their cases with each other. As a young lawyer, I would seek out mentors both inside and outside my firm to give me good advice on how to handle cases generally and about specific issues relative to my cases. In those days, we discussed cases by phone or over lunch. We also discussed questions about cases with speakers at CLE presentations.
After the advent of email, lawyers began joining listervs to exchange information. Most of the listervs have restrictive membership rules to prevent information from getting back to opposing counsel. In my experience with listervs though, I often thought many lawyers were too free with the facts of their particular cases and divulged confidential client information.
Now, social media is becoming the predominant way that people exchange information about both important and trivial matters. Besides websites, lawyers market their services most heavily on Facebook and LinkedIn. Facebook and LinkedIn have become vehicles for lawyers to exchange information about their cases. So the question becomes: Can lawyers ethically use social media to exchange information about their cases? State Bar of Texas Ethics Opinion Number 673 issued in August 2018 answers that question.
The opinion acknowledges the custom of lawyers discussing their cases with other lawyers to "test their knowledge, exchange ideas, and broaden their understanding of the law, with the realistic goal of benefiting their clients" but cautions lawyers not to violate their professional obligations under the Texas Disciplinary Rules.
Rule 1.05 covers both "privileged confidential information" and "unprivileged confidential information." Most lawyers are sensitive to attorney/client communications that are privileged confidential information. But, unprivileged confidential information is much broader and can cover subjects that have been in the newspaper or on the evening news. To get good advice on an issue, a lawyer obviously must disclose sufficient facts about the case to make sure the issue is clearly framed. By doing so, the lawyer flirts with violating Rule 1.05.
With this risk in mind, lawyers need to rely on the application of the exceptions to the rule for revealing unprivileged client information. Subpart (d)(1) says a lawyer may reveal unprivileged confidential information "when impliedly authorized to so in order to carry out the representation", and subpart (d)(2) says a lawyer may reveal unprivileged confidential information when the lawyer has reason to believe it is necessary to do so in order to "carry out the representation effectively." Per the Committee, these exceptions permit a lawyer to reveal a limited amount of unprivileged client information to lawyers outside the lawyer's firm without the client's express consent when the lawyer reasonably believes that the revelation will further the representation and when it is not reasonably foreseeable that the client will be prejudiced.
In setting this standard, the Committee set out the following guidelines:
- An inquiring lawyer may reveal unprivileged client confidential information only to the extent necessary for effective consultation for the client's benefit. If an effective consultation cannot be held without divulging any unprivileged confidential information, then the lawyer should not divulge it.
- If the lawyer determines that unprivileged client information must be revealed, the lawyer should use a hypothetical that does not reveal the identity of the client or information identifiable to the client. If discussion about the hypothetical threatens to harm or prejudice the client, then Rule 1.05 may be violated absent client consent to the disclosure. The lawyer should evaluate the risk of prejudice to the client by assuming that the inquiry might be disclosed to adverse counsel or the public.
- A lawyer should never disclose privileged confidential information specific to an identifiable client that foreseeably might prejudice the client without informed client consent.
- If the client instructs the lawyer not to reveal confidential information, then the lawyer may not do so even if the exceptions would otherwise apply.
- A lawyer who intends to reveal unprivileged confidential information to a responding lawyer may wish to consider getting the responding lawyer's agreement to keep the information confidential. If no agreement for confidentiality is obtained, the inquiring lawyer should take that fact into consideration before revealing confidential information.
The opinion protects the responding lawyer by stating no attorney client relationship is formed between the inquiring lawyer's client and the responding lawyer and absent an agreement to the contrary a responding lawyer has no duty of care, loyalty or confidentiality to the client.
The Committee also excepted communications between an inquiring lawyer and a private ethics lawyer or the State Bar of Texas Ethics Helpline from the scope of the opinion.
In conclusion, while the opinion does not ban lawyer to lawyer communications about cases on social media, lawyers need to be very careful when framing social media inquiries. The inquiring lawyer needs to carefully consider whether unprivileged client information must be revealed, and if so, either craft a hypothetical to avoid doing so or get client consent.


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Category: Conventional Tractor Trucks
Manufacturer: Mack
Model: R
Description: 2012 MACK CHU613 DAY CAB, MACK MP8505C ENGINE, 525 HP, EATON 13 SPD., 373 REAR END, AR, JAKES, CRUISE, 11R24.5 FRUITLAND 500 DIRECT DRIVE PUMP, 11R24.5, 321,736 MI. VIN:1M1AN07Y6CM011065 (G)
Construction Equipment Guide
470 Maryland Drive
Fort Washington, PA 19034
Construction Equipment Guide covers the nation with its four regional newspapers, offering construction and industry news and information along with new and used construction equipment for sale from dealers in your area. Now we extend those services and information to the internet. Making it as easy as possible to find the news and equipment that you need and want.
Privacy Policy
All rights reserved. Copyright 2020. Reproduction of materials appearing on this Web site is strictly prohibited without written permission.


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Status: Closed
Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
Be the first lehighvalleylive.com user to add photos or videos of Prevent Is Key
Prevent Is Key is located at 25 W Main St, Rockaway, NJ. This business specializes in Other Organizations.


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Are you over 50 and making a difference? Apply for the AARP Purpose Prize by April 30.
by Katharine Greider, From the AARP Bulletin Print Edition, September 3, 2007
Comments: 0
For Americans who undergo nonemergency procedures overseas, insurance coverage is still the exception to the rule. Liability is one concern. But as the health travel trend grows and U.S. medical costs skyrocket, insurers—and employers—are testing the waters.
Some pioneers:
United Group Programs, Inc., a consultant and third-party administrator of employer health plans based in Boca Raton, Fla., offers insurance for patients at a handful of overseas hospitals at a sharply reduced cost to the employer. Patients pay no deductibles or copayments. Nearly 50 companies, ranging in size from 150 to 100,000 employees, have signed on, says Jonathan Edelheit, vice president of United Group.
Patients must sign liability waivers, but their follow-up care at home is covered, Edelheit says. United Group is also launching an international preferred provider organization with negotiated discounts at participating hospitals, and it plans to introduce financing for individuals seeking medical care abroad.
BlueCross BlueShield of South Carolina offers coverage to its 1.5 million members for major procedures performed at Bumrungrad International Medical Center in Bangkok. The plan, offered through a subsidiary called Companion Global Healthcare, will soon expand to include a hospital in Europe, and possibly in Singapore and India, says David Boucher, the insurer's assistant vice president for health care services.
Blue Shield of California and Health Net of California offer inexpensive "cross-border" policies allowing members to get care in Mexico.
Legislators in West Virginia and Colorado recently introduced bills that would give state employees cost-saving incentives under their health plans to travel overseas for major procedures.
But going abroad for treatment isn't always an easy sell. When Blue Bridge Paper Products of North Carolina proposed to send an employee to India for surgery last year, the United Steelworkers (USW) union objected, and the plan was dropped. Stan Johnston, director of the USW's District 9, says a major worry was that eventually insurers might mandate travel abroad as a cost-saving measure.
"Let's assume that it was your 80-year-old mother or 3-year-old grandchild who was being outsourced to India or Thailand," he says. "That puts a whole different dynamic on the circumstance, and people need to think deeply about what this means."
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Stories Between Two Novellas
On Sale: 04/24/2018
Spend $49 and get FREE shipping on HC.com
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A striking new collection of ten short stories and two novellas that explores the idea of property in every meaning of the word, from the acclaimed New York Times bestselling author of the National Book Award finalist So Much for That and the international bestseller We Need to Talk About Kevin.
Intermingling settings in America and Britain, Lionel Shriver's first collection explores property in both senses of the word: real estate and stuff. These pieces illustrate how our possessions act as proxies for ourselves, and how tussles over ownership articulate the power dynamics of our relationships. In Lionel Shriver's world, we may possess people and objects and places, but in turn they possess us.
In the stunning novella "The Standing Chandelier," a woman with a history of attracting other women's antagonism creates a deeply personal wedding present for her best friend and his fiancée—only to discover that the jealous fiancée wants to cut her out of their lives. In "Domestic Terrorism," a thirty-something son refuses to leave home, resulting in a standoff that renders him a millennial cause célèbre. In "The ChapStick," a middle-aged man subjugated by service to his elderly father discovers that the last place you should finally assert yourself is airport security. In "Vermin," an artistic Brooklyn couple's purchase of a ramshackle house destroys their once-passionate relationship. In "The Subletter," two women, both foreign conflict junkies, fight over a claim to a territory that doesn't belong to either.
Exhibiting a satisfying thematic unity unusual for a collection, this masterful work showcases the biting insight that has made Shriver one of the most acclaimed writers of our time.
- Imprint: Harper
- On Sale: 04/24/2018
- Trimsize: 6.00 in (w) x 9.00 in (h) x 1.09 in (d)
- Pages: 336
- List Price: 26.99 USD
- BISAC1: FICTION / Short Stories (single author)
- BISAC2: FICTION / Family Life
- BISAC3: FICTION / Literary
"Shriver... is a brilliant satirist and virtuosic writer....even if Property isn't your dream house, it's a diverting enough place to spend an afternoon or two." - Associated Press
"The collection, which concentrates on middle-class Brits and Americans, is bookended by two richly detailed and sardonic novellas.... Shriver's stories will make readers laugh when they feel they shouldn't, and the uniting theme of houses and humans works exceedingly well, turning up new wrinkles with each successive story." - Publishers Weekly (starred review)
"Two novellas and ten stories by the author of We Need to Talk About Kevin skewer the absurdities of materialism, wherein possessions substitute for meaning and power is exerted by, say, being 30 years old and refusing to leave your parents' house." - O Magazine, 10 Titles to Pick Up Now
"A collection of two novellas and 10 short stories from the author of the explosive "We Need to Talk About Kevin." Set in Britain and the US, the stories explore the relationship between people, property and other "stuff."" - New York Post, This Week's Must-Read Books
"Award-winning Shriver's enthusiastic audience will delight in her clever and literary analyses of the spaces we occupy, and how they're all too often no broader than a knife's edge." - Booklist


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For our project we ordered an exclusive clicker brick, which
was specially developed in Germany for us.
The brick is called EGODOM.
It has totally unique aesthetics: heterogeneity of texture and
colour. There are no chemical additives or colourants. The
brick is made of natural clay. At the same time it has low
water absorption and high frost-resistance.
Read more:
We use eco-friendly aluminum window construction
with increased energy efficiency from leading
international companies. Our supplier is the
leader in this field: Schuco Germany.
Read more:
Each element of our project meets high international standards:
from Quick-mix (a special solvent for brickwork) to Stratobel
Energy N 66.2 (sun-protective multilayer windows on the facade).
We can tell you everything in details about elevators, pipes,
ventilation and each object in the building. We would be
happy to answer Your questions.
+7 495 2345 720
order a callback


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L/N Little Nemo
Large WWII V for Victory Pin Gold Tone Red Lucite Sweetheart Pin Vintage Home Front Patriotic Jewelry
Regular price $175.00
Very large 1940's V for Victory pin. In very good condition. Age appropriate wear. Clasp works well. Signed L/N (Little Nemo).
Your fantastic brooch will be gift wrapped, well wrapped, and shipped with insurance and tracking.
Thank you.


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Wallace and Gromit are great for class. This 3 part video supports ELLs (English Language Learners) with text and materials.Part 2 Part 3
Resources for teaching HERE.
Downloads: 9406
Great Screen English. One of the reasons why showing a movie gets such a bad reputation particularly in places like Korean public schools is the tendency to subtitle in the students mother tongue which is simply killing time.
This is great for those after exams periods where the tendancy is just to swap DVD's and hope for the best.
Thank you for this!!!! This site is awesome, worth every cent!!
Thanks for the vote of confidence Andy. We've been around a long time and just recently went with the supporter fee/donation. But I like the fact that we can get self supporting but still be a teacher led community with and for worthy teachers everywhere.
Where can I find the A Close Shave videos to download, pls?
Above every video is a link that says, "Download Videos". Click this and it goes to our video files folder. Go to the appropriate folder and scroll to find the video. The folder for Wallace and Gromit videos is in "Videos For The Classroom Low".
You are right, not that easy to find so I'll also move it as a regular folder in the Video files folder you first go to. Sorry for any confusion.
Add a Remark


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Our lives are in God's hands. God has been faithful in the last 40 years of serving him as Anglican clergy. There have been many surprises along the road. He has worked all things for the good in ways that I would not always have imagined. (Romans 8:28 & Genesis 50:20)
Nana Allen, my maternal grandmother, was an amazing lady. She was a devout Anglican Christian who loved the Book of Common Prayer, and knew that something was being tampered with in the DNA of Anglicanism. Nana knew that I would become an Anglican priest, and told me this years before I even came to personal faith. She was very close to God and heard his still small voice. Nana's desire was to live until I became a deacon (which she did) and then to live until I became a priest (which she did). She died shortly before my throat operation on May 25th 1982 when God restored my voice. I wrote her funeral eulogy, but had to rely on Rev Harold McSherry to deliver it.
In the Anglican Church, they ordain you twice just to make sure that it sticks. 😉 My first ordination was on May 18th 1980 where I was ordained as a deacon by Archbishop David Somerville. I was wearing a new suit that I had been given as an ordination present. For my ordination as a priest on May 31st 1981, Archbishop Douglas Hambidge ordained me at St Philip's Church Dunbar. It was a challenging time because I was having speech therapy but my voice had not returned. My medical specialists assured Archbishop Hambidge that my voice would return in another month or so. When this did not happen, my medical specialists encouraged me to leave St. Philips on Oct 1st 1981 to take up full-time speech therapy. They were concerned that otherwise my voice might never come back. This was a very painful but needed transition. I was off work doing speech therapy for exactly one year on Oct 1st 1982 when I moved to St Matthew's Abbotsford as the assistant priest with Archdeacon Jack Major. Being at St Matthew's was life-transforming for me in untold ways.
The Hirds singing a song unto the Lord at St Matthias Oakridge
Absolutely foundational in our Christian walk and growth was our time at St Matthias Oakridge with the Rev Ernie Eldridge. Ernie+ encouraged us to use all of our gifts, especially the gift of music. Janice my wife is a professional musician who graduated from the UBC School of Music. We loved to sing together, especially with our singing group Morning Star. One of the unfortunate side-effects of my Botox treatments every three months is that while it helps my speaking, it limits my singing voice. My guitar playing has greatly improved after eight years of guitar lessons with Tony Chotem. So even though my singing is limited, I am still able to serve in the area of music ministry. When I get to heaven, I look forward to the complete restoration of both my speaking and singing voice. In the meantime, I am grateful that I am still able to preach and serve as a priest, after being told by my GP in 1981 that I would never preach again. Without the throat operation, the ongoing prayer, and the Botox treatments, this would have been my fate.


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Sunday, April 22, 2018
A 2 PWE Post
Cardboard. Cardboard is nice, too!
Once a Cub, busted some Topps Opening Day and he sent me a boatload in a pair of PWEs along with some other high quality Cub offerings.
Ryno! This first one is from Panini Chronicles, which I though was a very unique concept for a set.
I'm now up to 869 different Rynos. The 900 milestone is within reach if I want to pursue it.
We'll see though, because I have other ideas on how I'm going to spend all my card money this year. For example: I need to save some dough for when The Vogelmonster goes on his inevitable homerun tear and hitting streaks. Vogelbach will be the poster child for Topps NOW before the season is over. You heard it hear first!
Matt, thanks for the nice cardboard surprise in my mailbox last week. It was very much appreciated and I'll see what I can do about evening the score before too long.
Quick Update: I'm still not back to full blogging strength because school and my baseball coaching duties are demanding much of my time. Seniors graduate three weeks from today, which means the end of the school year isn't far behind. Baseball? Well, I'm hoping that goes into late May and/or early June. We're on a nice little winning streak right now and gave an undefeated team their first loss of the season yesterday. It was a good day. Overall, our record is 15-2 at the halfway point of the regular season. I'm hoping the second half of the season and the playoffs go as well as the first half!
Happy spring, everyone!
7 comments:
Same to you! And good luck with your teamReplyDelete
It sounds like your kids are rolling like the Red Sox. Good work, coach!ReplyDelete
What is that pavillion-type thing pictured in the OD card above the Traditions card?Replies
That is the new-ish Cubs park. It houses the team store, the trophy/awards room and front offices. The grassy area in front is where the Cubs hold friendly neighborhood activities on non game days, like yoga, ice skating in the winter and etc.Delete
I like how Topps credits "Take Me Out To The Ballgame" to the Cubs, because of Harry, when Carey started the tradition on the south side.ReplyDelete
We've never known Topps to be very strong in the research department.Delete
That's exactly what I did with Quintana. Twice. Each were 2-case breaks and I came away with all base, haha.ReplyDelete
The 83 Happs were from a player break, too. The shiny one is from the bonus silver pack that came with buying a hobby box.
Also, the Baez and Happ from Series 1 are the "workout gear" SP photo variations.
I pulled the Sandbergs out of a mess of a box at the card show I just went to for myself and when I saw doubles, I figured you'd need them too.Replies
Also congrats on your team!Delete


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Design Style Series: Do We Need Design Styles?
When someone decides to make a house, it's never a new or a fresh decision. We dream of the kind of house we will have when we grow up or when we get married. Quite like the stereotype of women daydreaming about their marriage (which is true in certain cases); all of us also day-dream about what our house will look like.
We day dream of large homes with many rooms and beautiful decor.
The reality on the ground is something quite different. We live in small houses and have to always decorate on a budget. Shying away from words that describe us, doesn't help anyone. And the fact is that most of us belong to the bulky middle-class chunk of the Indian population.
Once we own a house, we head straight to google to look for images that we can replicate. Those of us who know about it head to Pinterest. We will look for small home design ideas and find absolutely beautiful images that seem to make living in a small house our new dream.
Now we are looking for budget decorating ideas and who would hire an interior designer for a budget home design, amirite!?
The end result although is never quite what we had in mind. Something is always off and we never know what.
Just like everything else in the world, interior design has categories. There are different styles of design and it is very important to maintain that through out the house. That is not to say that you can have hints of everything else. But the weightage that these receive should not be the same. Look at the pictures below. Which one would you prefer?
They both look good. But there is one definite winner. Ikea has used elements from the same style and they tied the room together with the correct accessories. The over all elements are the same. A solid wall, a couch, a coffee table, two different lighting sources, side-tables, greenery, singular wall adornment (mirror versus blank frame is debatable).
Still one is better than the other right? It's the combination of the things that matter.
Now, this is not a 'buy Ikea' post. This is to demonstrate the difference between what you see in your head versus what actually happens.
The most important thing to keep in mind, to have a picture-perfect home, is to decide on and stick to a particular design style.
Design Styles are indispensable to create a perfect home.
But there are way too many design styles out there, each distinct and yet the same.
Interior designing like all other things has categories and types. Some are based on the 'time' or the 'age' and some are based on the location of where they are usually found. Country is one design style that has clear evidence of the difference. French country home is very different from a British country home which is vastly different from an American country home!
For the upcoming posts, we will not segregate the different styles based on the so-called categories. But we will make the Indian version of those clear, in addition to the origins
As you go through the posts, you will find elements over-lapping or some might even look exactly the same. The reason is none of these styles was developed in isolation. All the styles of design and the types of furniture are all derived from or inspired by what preceded them. For example, if you google contemporary, modern,
Look at the gallery below, we collected pictures from different sources – some are contemporary, some minimal, some scandinavian, some modern. Bet its tough to say which is which!
This might have left you understandably confused. That was what we hoped for!! 😉
Design styles matter and they are confusing, that's why you have CUBSpaces!
Over the course of
Miloni Mehta
CUBS Editorial Team


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Personalized Metallic Foil Candy Jars
- Dimensions: jar 2.75"L x 1.75"W, label 1.25" diameter
- Materials: glass, metal lids, self-sticking labels
- Minimum order: 24
- Some assembly required
These personalized metallic foil candy jars are the sweetest way to share your love with wedding guests on your special day. Fill these personalized mini candy jars to the brim with your favorite sugary treat and set out on a favor table or as part of your place settings for a favor your guests will surely savor. Personalize each jar with one of our many delightful lid designs. Plus, you can include the bride's name, couple's names, date and/or a personal message of your own. These mini jars are made of durable glass and come complete with a metal screw-on lid. Simply peel n' stick the shiny labels on each lid, and refill and reuse to your heart's content!
Candy filler not included.
Estimated Production Time: 2-4 business days; rush ships within 1 business day
Click chart to enlarge
Click chart to enlarge


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Florentine Corner #C-162 Premium Finish
Availability: 3-4 weeks
Frame Size: 23″ x 19″
Thickness: 1″
This Florentine ceiling corner can also be used as a wall panel Focal Point. It is shown in a Premium Finish which uses up to 9 custom colors.
Product Information
- Corner dimensions 23″ x 19″
- Hand cast in urethane resin
- Suggested screws for installation – #6 flathead
- Screws required 4 wall/4 ceiling
- Premium Finish up to 9 custom colors
- Has cabochon settings used for installation screws and decorative crystals or semi-precious stones
Urethane Resin
To discuss your Premium Finish call and speak with our Artist/Designer. Have information available about your colors. We can work with all major paint brands. Samples of fabrics and digital images of the room and furnishings give our Artist the information necessary to develop your custom Premium Finish.
1. All returns require an RMS number. Please call 410-867-0790 within two weeks of your purchase to request an RMS number. Merchandise must be returned within two weeks after receiving your RMS number.
2. There is up to a 25% restocking/handling charge on returns, based upon the list price of the item online.
3. Please insure all returns for the full list amount. If any of the returned items are damaged in shipping, you will have to collect insurance from the shipper. Refunds will not include shipping charges.
4. Items returned because they were improperly measured or ordered without reading or understanding how items are sized will be subject to a 25% restocking charge, and given a store credit. This credit can be used to re-order the correct size.
5. Orders that involve custom work, custom painting (not one of our finishes), custom cutting, or custom additions cannot be cancelled, changed or returned. Filter Grilles cut to size, Speaker Grilles with surrounds added, or any grille painted in finishes other than Beaux-Arts Classic Products standard finishes, are considered custom items. All Recessed Chandeliers are considered custom items.
6. All of our products are shipped by FedEx Ground and international by FedEx International. Some of our larger grilles are considered oversized and, therefore, assessed additional charges for crating."


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We provide the following services:
- Soil surveys to determine the suitability of land for the establishment of a vineyard.
- Farm planning (rootstock suitability, soil preparation, cultivar recommendations, block design, etc).
- Information regarding the performance of clones for specific regions and sites.
- Viticultural consulting.
- Follow-up service after establishment of new plantings.
- Regular field days for clients sharing new and up to date information regarding new varieties, rootstocks, market trends, etc.
- Managing certain breeding programs as well as producer contracts with the table grape producers.
Contact Johan Wiese, Andrew Teubes or Koos van der Merwe:
Tel 021 864 1018 Fax 021 864 3689 e-mail email@example.com www.vgnurseries.co.za


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To use contract staff or to hire permanent employees, that is the question. Whether you're trying to fill a new role, planning to scale up, down or sideways, understanding contract vs employee pros and cons is absolutely essential. With contracting on the rise and the gig economy disrupting ..Read More
If you're tossing up whether or not you should use a recruitment agency to hire a new employee, we're here to set the record straight. Below are five major reasons not to use a recruiter. We should know. We are recruiters! You want to utilise the network closest to you Recruiting someone ..Read More
Mindfulness is the mental state achieved by focusing on the present, while acknowledging and accepting what is happening around you. There's a reason it has taken the world by storm. Mindfulness in the workplace has been shown to significantly increase productivity, strengthen communication, b..Read More
Ladies, did you know there are loads of high-paying job opportunities in one of Australia's largest industries? With an average salary of $109,325, it is one of the highest paying industries in the country. In fact, the industry is experiencing such rapid growth and simultaneous attrition com..Read More
Nicole * is a single mother on Centrelink. She's desperate to find work and willing to do what it takes to feed her daughter and keep a roof over their heads. Although she has a range of useful skills, Nicole isn't focused on pursuing her passion or climbing the corporate ladder. She has been l..Read More
Watch: Full recording of The Future of Innovation and Technology, with guest speaker Charlie Day. ..Read More


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Zone Nth Wales | DiBArt added on September 15, 2013 | Answered
I had a thriving Lavatera. This year at flowering time it started to droop and die. I cut it right back and it does have new foliage appearing. Does anyone know what happened?
Certified GKH Gardening Expert
Certified GKH Gardening Expert
Answered on September 24, 2013
It could have been several things. A fungus or a root problem sounds most likely as when you cut it back, it started to recover.
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text:
- Location
Central Otago
- Duration
- Nine days, 9.00am-3.00pm
- Delivery
On campus
- Start
- 10 & 11 June, 15-18 June, 22 July, 3 & 4 August 2020
- Apply
- Anytime
Learn how to prune and train fruit trees, including apples, pears, cherries, plums, apricots, peaches and nectarines.
This course is heavily practical and you will be required to prune a large number of trees to gain a good understanding of the topic.
- Training systems used in a range of fruit tree types
- Pruning techniques and responses to pruning
- Tools and equipment used in pruning
- Health and safety in pruning
This is a nine-day course. For 2020 the dates are: 10 & 11 June, 15-18 June, 22 July, 3 & 4 Aug (9 days in total).
For more information
Please contact firstname.lastname@example.com or phone Central Campus on 0800 765 9276.
Materials and clothing
Please wear warm clothing suitable for learning in an outdoor environment. All materials and equipment are supplied for this course.
You do not have to sit an assessment and will receive a Certificate of Attendance to demonstrate that you completed the course.
To apply
Please click the apply button in the top right-hand corner of this webpage to begin the application process.
For help with applications, please contact firstname.lastname@example.com
While every effort is made to ensure that this sheet is accurate, Otago Polytechnic reserves the right to amend, alter or withdraw any of the contained information. The fees shown in this document are indicative ONLY. Both domestic and international fees are subject to change and are dependent on the development and implementation of Government policies. Please note that additional fees may from time to time be required for external examination, NZQA fees and/or additional material fees.


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First lap was getting a feel for the kart and pedals. George didn't hear the bit about no over-taking during this lap. Then it was 15 minutes of what felt like an hour of driving as fast as you can (unless you're Sue, who pootled). The karts get up to speeds of 40mph on what was quite a small track with loads of bends. Tom and Jamie raced each other all the way and were the quickest with Tom achieving the fastest lap recorded for that day, let alone our session. It should be noted however, that they weren't without their pushing and shoving of other drivers on the track. There were a couple of wipeouts and a few banners of 'no bumping' flashed up to certain racers.
Afterwards, we went bowling and split into teams of 4. Some started off well, others came through for the finish. Some struggled with the 'mind games', others struggled with lifting a bowling ball after the driving escapade... A solid effort with Jamie achieving the highest score and his team won on points.


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Western blot analysis of cell extracts from various cell types using SRC-1 (128E7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using SRC-1 (128E7) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from LS180 cells treated with calcitriol (10nM, 3 hours) and either SRC-1 (128E7) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Upstream Primers #25661 and SimpleChIP® Human Nanog Promoter Primers #95064. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
|Product #
|100 µl
|$ 268
|H M R Mk
|MW (kDa)
|Rabbit IgG
Product Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
|Western Blotting
|Immunohistochemistry (Paraffin)
|Chromatin IP
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 5 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised October 2016
Protocol Id: 10
This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity utilizing Protein A magnetic separation.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
10X Cell Lysis Buffer: (#9803) To prepare 10 ml of 1X cell lysis buffer, add 1 ml cell lysis buffer to 9 ml dH2O, mix.
NOTE: Add 1 mM PMSF (#8553) immediately prior to use.
A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads. Pre-clear enough lysate for test samples and isotype controls.
IMPORTANT: Pre-wash #73778 magnetic beads just prior to use:
Carefully remove the buffer once the solution is clear. Add 500 μl of 1X cell lysis buffer to the magnetic bead pellet, briefly vortex to wash the beads. Place tube back in magnetic separation rack. Remove buffer once solution is clear. Repeat washing step once more.
IMPORTANT: The optimal lysate concentration will depend on the expression level of the protein of interest. A starting concentration between 250 μg/ml-1.0 mg/ml is recommended.
IMPORTANT: Appropriate isotype controls are highly recommended in order to show specific binding in your primary antibody immunoprecipitation. Use Normal Rabbit IgG #2729 for rabbit polyclonal primary antibodies, Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 for rabbit monoclonal primary antibodies, and Mouse (G3A1) mAb IgG1 Isotype Control #5415 for mouse monoclonal primary antibodies. Isotype controls should be concentration matched and run alongside the primary antibody samples
Proceed to one of the following specific set of steps.
NOTE: To minimize masking caused by denatured IgG heavy chains (~50 kDa), we recommend using Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (#45262) or Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (#3678) (or HRP conjugate #5127). To minimize masking caused by denatured IgG light chains (~25 kDa), we recommend using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (#3678) (or HRP conjugate #5127).
posted December 2008
revised April 2018
Protocol Id: 410
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114
|SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653
|SignalStain® DAB Substrate Kit #8059
|SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted June 2005
revised April 2020
Protocol Id: 303
Specific for product: SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005.
Reagents Included:
Reagents Not Included:
|This ! signifies an important step in the protocol regarding volume changes based on the number of immunoprecipitation preparations (IP preps). One IP prep is defined as 4 x 106 tissue cultured cells or 25 mg or disaggregated tissue.
|This !! signifies an important step to dilute a buffer before proceeding.
|This is a safe stopping point in the protocol, if stopping is necessary.
When harvesting tissue, remove unwanted material such as fat and necrotic material from the sample. Tissue can then be processed and cross-linked immediately, or frozen on dry ice and stored at -80°C for processing later. For optimal chromatin yield and ChIP results, use 25 mg of tissue for each immunoprecipitation to be performed. The chromatin yield does vary between tissue types and some tissues may require more than 25 mg for each immunoprecipitation. Please see Appendix A for more information regarding the expected chromatin yield for different types of tissue. One additional chromatin sample should be processed for Analysis of Chromatin Digestion and Concentration (Section IV). If desired, five additional chromatin samples should be processed for Optimization of Chromatin Digestion (Appendix B).
(!) All buffer volumes should be increased proportionally based on the number of IP preps in the experiment.
For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed (at least 12 X 106 cells are required in order to include positive and negative controls). For HeLa cells, one IP is equivalent to half of a 15 cm culture dish containing cells that are 90% confluent in 20 ml of growth medium. One additional sample should be processed for Analysis of Chromatin Digestion and Concentration (Section IV). Since every cell type is different, we recommend including one extra dish of cells in experiment to be used for determination of cell number using a hemocytometer or cell counter.
(!) All buffer volumes should be increased proportionally based on the number of 15 cm tissue culture dishes (or 20 ml suspension cells) used.
(!) All buffer volumes should be increased proportionally based on the number of IP preps in the experiment.
(!!) IMPORTANT: Once in solution, store 1M DTT at -20°C.
NOTE: For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration. Over-digestion of chromatin may diminish signal in the PCR quantification. Under-digestion of chromatin may lead to increased background signal and lower resolution. Adding too little chromatin to the IP may result in diminished signal in the PCR quantification. A protocol for optimization of chromatin digestion can be found in Appendix B.
For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin (as determined in Section IV) per immunoprecipitation. This should be roughly equivalent to a single 100 µl IP prep from 25 mg of disaggregated tissue or 4 x 106 tissue culture cells. Typically, 100 µl of digested chromatin is diluted into 400 µl 1X ChIP Buffer prior to the addition of antibodies. However, if more than 100 µl of chromatin is required per IP, the cross-linked chromatin preparation does not need to be diluted as described below. Antibodies can be added directly to the undiluted chromatin preparation for immunoprecipitation of chromatin complexes.
(!) All buffer volumes should be increased proportionally based on the number of immunoprecipitations in the experiment.
NOTE: Most antibodies from Cell Signaling Technology work optimally between 1 and 2 ug per IP sample. In the case where there are multiple samples with varying concentrations, it is best to match the negative control Normal Rabbit IgG #2729 to the highest antibody concentration.
(!) All buffer volumes should be increased proportionally based on the number of immunoprecipitations in the experiment.
|Primer length:
|24 nucleotides
|Optimum Tm:
|Optimum GC:
|Amplicon size:
|150 to 200 bp (for standard PCR)
|80 to 160 bp (for real-time quantitative PCR)
|Volume for 1 PCR Reaction (18 µl)
|Nuclease-free H2O
|12.5 µl
|10X PCR Buffer
|2.0 µl
|4 mM dNTP Mix
|1.0 µl
|5 µM RPL30 Primers
|2.0 µl
|Taq DNA Polymerase
|0.5 µl
|Initial Denaturation
|5 min
|30 sec
|30 sec
|30 sec
|Repeat Steps b-d for a total of 34 cycles.
|Final Extension
|5 min
|Volume for 1 PCR Reaction (18 µl)
|Nuclease-free H2O
|6 µl
|5 µM RPL30 Primers
|2 µl
|SimpleChIP® Universal qPCR Master Mix #88989
|10 µl
|Initial Denaturation
|95°C 3 min
|95°C 15 sec
|Anneal and Extension:
|60°C 60 sec
|Repeat steps b and c for a total of 40 cycles.
Analyze quantitative PCR results using the software provided with the real-time PCR machine. Alternatively, one can calculate the IP efficiency manually using the Percent Input Method and the equation shown below. With this method, signals obtained from each immunoprecipitation are expressed as a percent of the total input chromatin.
Percent Input = 2% x 2(C[T] 2%Input Sample - C[T] IP Sample)
C[T] = CT = Threshold cycle of PCR reaction
The immuno-enriched DNA samples prepared with this kit are directly compatible with ChIP-seq. For downstream NG-sequencing DNA library construction, use a DNA library preparation protocol or kit compatible with your downstream sequencing platform. For sequencing on Illumina® platforms, we recommend SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 and its associated index primers SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) #29580 or SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers) #47538.
When harvesting cross-linked chromatin from tissue samples, the yield of chromatin can vary significantly between tissue types. The table to the right provides a range for the expected yield of chromatin from 25 mg of tissue compared to 4 x 106 HeLa cells, and the expected DNA concentration, as determined in Section IV of the protocol. For each tissue type, disaggregation using a Medimachine (BD Biosciences) or a Dounce homogenizer yielded similar amounts of chromatin. However, chromatin processed from tissues disaggregated using the Medimachine typically gave higher IP efficiencies than chromatin processed from tissues disaggregated using a Dounce homogenizer. A Dounce homogenizer is strongly recommended for disaggregation of brain tissue, as the Medimachine does not adequately disaggregate brain tissue into a single-cell suspension. For optimal ChIP results, we recommend using 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation; therefore, some tissues may require harvesting more than 25 mg per each immunoprecipitation.
|Total Chromatin Yield
|Expected DNA Concentration
|20-30 µg per 25 mg tissue
|200-300 µg/ml
|10-15 µg per 25 mg tissue
|100-150 µg/ml
|8-10 µg per 25 mg tissue
|80-100 µg/ml
|2-5 µg per 25 mg tissue
|20-50 µg/ml
|2-5 µg per 25 mg tissue
|20-50 µg/ml
|10-15 µg per 4 x 106 cells
|100-150 µg/ml
Optimal conditions for the digestion of cross-linked chromatin DNA to 150-900 base pairs in length is highly dependent on the ratio of Micrococcal Nuclease to the amount of tissue or number of cells used in the digest. Below is a protocol for determination of the optimal digestion conditions for a specific tissue or cell type.
|Possible Causes
|1. Concentration of the digested chromatin is too low.
|Not enough cells added to the chromatin digestion or nuclei were not completely lysed after digestion.
If DNA concentration of the chromatin preparation is close to 50 µg/ml, add additional chromatin to each IP to give at least 5 µg/IP and continue with protocol.
Count a separate plate of cells before cross-linking to determine an accurate cell number and/or visualize nuclei under microscope before and after sonication to confirm complete lysis of nuclei.
|2. Chromatin is under-digested and fragments are too large (greater than 900 bp).
Cells may have been over cross-linked. Cross-linking for longer than 10 min may inhibit digestion of chromatin.
Too many cells or not enough Micrococcal Nuclease was added to the chromatin digestion.
Perform a time course at a fixed formaldehyde concentration. Shorten the time of cross-linking to 10 min or less.
Count a separate plate of cells before cross-linking to determine accurate cell number and see Appendix B for optimization of chromatin digestion.
|3. Chromatin is over-digested and fragments are too small (exclusively 150 bp mono-nucleosome length). Complete digestion of chromatin to mono-nucleosome length DNA may diminish signal during PCR quantification, especially for amplicons greater than 150 bp in length.
|Not enough cells or too much Micrococcal Nuclease added to the chromatin digestion.
|Count a separate plate of cells before cross-linking to determine accurate cell number and see Appendix B for optimization of chromatin digestion.
|4. No product or very little product in the input PCR reactions.
Not enough DNA added to the PCR reaction or conditions are not optimal.
PCR amplified region may span nucleosome-free region.
Not enough chromatin added to the IP or chromatin is over-digested.
Add more DNA to the PCR reaction or increase the number of amplification cycles.
Optimize the PCR conditions for experimental primer set using purified DNA from cross-linked and digested chromatin. Design a different primer set and decrease length of amplicon to less than 150 bp (see primer design recommendations in Section VIII).
For optimal ChIP results add 5-10 µg chromatin per IP. See recommendations for problems 1 and 3 above.
|5. No product in the positive control Histone H3-IP RPL30 PCR reaction.
Not enough chromatin or antibody added to the IP reaction or IP incubation time is too short.
Incomplete elution of chromatin from Protein G beads.
Be sure to add 5-10 µg of chromatin and 10 µl of antibody to each IP reaction and incubate with antibody over-night and an additional 2 h after adding Protein G beads.
Elution of chromatin from Protein G beads is optimal at 65°C with frequent mixing to keep beads suspended in solution.
|6. Quantity of product in the negative control Rabbit IgG-IP and positive control Histone H3-IP PCR reactions is equivalent.
Too much or not enough chromatin added to the IP reaction. Alternatively, too much antibody added to the IP reaction.
Too much DNA added to the PCR reaction or too many cycles of amplification.
Add no more than 15 µg of chromatin and 10 µl of histone H3 antibody to each IP reaction. Reduce the amount of normal rabbit IgG to 1 µl per IP.
Add less DNA to the PCR reaction or decrease the number of PCR cycles. It is very important that the PCR products are analyzed within the linear amplification phase of PCR. Otherwise, the differences in quantities of starting DNA can not be accurately measured.
|7. No product in the Experimental Antibody-IP PCR reaction.
Not enough DNA added to the PCR reaction.
Not enough antibody added to the IP reaction.
Antibody does not work for IP.
Add more DNA to the PCR reaction or increase the number of amplification cycles.
Typically a range of 1 to 5 µg of antibody are added to the IP reaction; however, the exact amount depends greatly on the individual antibody.
Increase the amount of antibody added to the IP. Find an alternate antibody source.
posted December 2011
revised June 2018
Protocol Id: 82
SRC-1 (128E7) Rabbit mAb detects endogenous levels of total SRC-1 protein (all three isoforms). The antibody does not cross-react with other SRC proteins, including SRC-2 and SRC-3.Species Reactivity:
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SRC-1 protein.
There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).
Explore pathways + proteins related to this product.
UniProt ID: Q15788
Entrez-Gene Id: 8648
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.


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Maggiano's Italian Restaurants Near Prairie Village
Salute! Dine At Maggiano's Italian Restaurant Near Prairie Village
Take a seat and enjoy a wholesome Italian meal at Maggiano's Little Italy. From season-fresh salads to classic pastas, we combine the flavors of Nonna's authentic Italian recipes with the joy of friends and family. Find a Maggiano's Italian restaurant near you in Prairie Village today and reserve a table online or over the phone!
Maggiano's Banquet Halls:
The Perfect Place For Your Event
For Italian food near Prairie Village that doesn't disappoint, come to Maggiano's Little Italy. Book a reservation online or visit your local restaurant for lunch for two, dinner with the whole office, or a quick bite at the bar with friends. No matter the meal, the day, or the size of your party, you'll always be treated like a special guest at Maggiano's. Here, every guest is family!
From appetizing antipasto dishes to Chef-prepared cakes and cookies, we believe that simplicity is at the core of Italian cooking. To really bring out the full-bodied flavors at the heart of our handcrafted recipes, our chefs use only the freshest season-ready meats, vegetables, cheeses, and grains. With gluten-free, vegetarian, vegan, and classic Italian fare, Maggiano's has a dish for everyone. Order a Chef-prepared pasta entree, refreshing salad, hearty soup, or cheesy flatbread and get ready for an Italian-American treat!
Here at Maggiano's, we think there's more to "eating Italian style" than simply eating. It's about reconnecting with friends and family, unwinding after a tough day, and savoring each and every bite of food. It's never rushed and it's always delicious. At our restaurants, your next lunch or dinner can be memorable, but so can your next private event or special occasion. With elegantly decorated banquet halls and event spaces, and an attentive staff to match, Maggiano's is the ideal event venue near Prairie Village for any type of occasion. Whether you're hosting an anniversary dinner, bridal brunch, or need a large space to celebrate a quinceanera or bar/bat mitzvah, let our Private Dining Managers make your next gathering easy, memorable, and above all—delicious!
Call your local Maggiano's today or make a reservation online to see why we're nothing like other Italian restaurants near Prairie Village. Experience something exceptional at Maggiano's!


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Happy Plugs
Happy Plugs Ltd. Unit A, 7/F 78 Hung To Road Kwun Tong
Hong Kong
Phone:+46 855923801
Company Presentation
About Us
Happy Plugs is designed in Sweden, known for its vibrant fashion and music scenes. We created Happy Plugs with the ambition of transforming essential tech accessories into fashion must-haves. We keep it simple and elegant, but still stylish and affordable. Our Happy Plugs are made to fit your personality, your style and your devices.
Happy Plugs is the global market leader in fashion tech, available at over 10.000 retailers in 70 countries worldwide - making it a global phenomenon. Happy Plugs products are available in the most prestigious concept stores like Nous and Le Bon Marche in Paris, Selfridges and Harrods in London, Restir and Loft in Tokyo to Blomingdales in the USA and Urban Outfitters' stores worldwide.
Chamber Representative
Andreas Vural


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This week on Money For the Rest of Us Plus we answer questions from members on investing in high yield municipal bond funds and ETFs, worries about a bubble, a substitute holding for the DoubleLine Total Return Bond Fund and investing in sector specific real estate investment trust funds and ETFs.
Become A Member
If you would like to listen to this episode of Money For the Rest of Us Plus, you can do so by becoming a member of the Money For the Rest of Us Hub.
Learn More About Membership Options »


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Student Leadership
Leading with enthusiasm and charisma.
At Kikino School, students have plenty of opportunities to take part in leadership programs. Some programs are seasonal, and some last all year. Students can join as many leadership programs as they want and/or do not have to join any at all. Attendance and behaviour are considerations for remaining in a leadership program.
For this school year, our leadership groups are:


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WWU names Lake resident a 2017-18 Outstanding Graduate
Lake Stevens Ledger
Katelyn Brower of Lake Stevens graduated magna cum laude in June with a Bachelor of Science degree and as the Outstanding Graduate in Geology. She is also a first-generation university graduate.
At Western, she served as a curator and research assistant to digitize, electronically document and catalog Western's extensive geologic natural history collection and presented her work at the 2017 Geological Society of America annual meeting. As the Northwest Geological Society's first at-large student board member, Brower helped plan the February 2018 Geohazards Symposium in Seattle. She is also a student liaison with the Pacific Northwest section of the American Institute of Professional Geologists and the webmaster of the Pacific Northwest Association for
Women Geoscientists.
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Wednesday, August 1, 2018 - 11:03am


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With the recent blow-it-outta-the-water opening of Good Times at Davey Wayne's, "secret" and "hidden" bars are about to start coming up in every conversation again. To help ensure you've got your secret bar basics down, here's a beginner's guide to every LA bar that's -- in some way (hidden entrance!) or another (no address!) -- secret. Shhhhhh.
Send Foodz: LA Gems
Blind Barber
Culver City
This NYC-imported cocktail bar features both award-winning mixologists and a where-the-hell-is-it entrance, but what makes it special is the fact that it's one of the only places in town where you can get an old-fashioned Flattop and an Old Fashioned on the same visit.
How to get in: Go through the back door of the barbershop. Maybe get your haircut first though?
Lock and Key
Even if there weren't a hidden entrance, this bar's facade -- at Vermont and Third -- is so indistinct, you'd think it was a whatever dive, rather than a classy cocktail bar. Add to that an Alice in Wonderland puzzle to get in, and you've hit K-town's best-or-second-best (see below) secret bar.
How to get in: Go through the front door... and then figure out which doorknob to turn to ACTUALLY get in.
Seventy7 Lounge
Culver City
You'd think the whole there's-no-name-and-it's-in-a-back-alley thing would have gotten old the minute Heather Graham did, but nope: Seventy7 Lounge continues the Swingers thing with stiff drinks, burlesque shows, and an entrance that's so money, it doesn't even know it.
How to get in: Find the cocktail sign in the alley -- there's a bouncer behind that door.
The Red Door
Toluca Lake
You may have already guessed that the name of this kinda Latin-themed lounge in the Valley doesn't have any signage, just, uh, red doorage; what's not totally clear is that in addition to delicious drinks, they also have an excellent food menu.
How to get in: Go to Robano's Pizza. Walk down the alley. Find the red door.
Because one great whiskey bar wasn't enough, Seven Grand now houses a second great whiskey bar -- albeit one that's super-tiny, super-secret, has a whiskey-locker program for your personal stash, and only serves three mixed drinks.
How to get in: There's a phone, and a light, with instructions in English... and Japanese.
The Writer's Room
The oontzery the Supper Club shares a wall (...and a hidden door) with this industry-hang cocktail lounge from super-awesome bartender Daniel Nelson and his crew, with vintage couches and mirrors and lots and lots of very dark corners. To drink in.
How to get in: Go behind Musso & Frank, and find the very huge and intimidating dude with the list. Be on it -- or be convincing.
La Descarga
In some ways, the grandfather of the secret-LA-bar, La Descarga is the Houston Brothers' ode to Havana in the 1950s, with rum drinks, a cigar smoking patio, and floorshows from live jazz bands with very, very live dancers.
How to get in: It helps to have a reservation -- so make one, and then the doorman will show you into an office, where you'll be told the rules, and shown the... closet?
The Varnish
This kinda-cramped-in-a-good-way speakeasy's in the back of one of LA's longest-running restaurants, and feels like a step back in time, thanks to wooden seats, stiff drinks, and music -- like Sinful Sundays, their end-of-the-weekend ode to dirty songs from the '20s.
How to get in: First, get a sandwich (and a Moscow Mule, if it's gonna be that kind of night) at Cole's, then continue to the door in the back, and turn the knob. Come early -- seats are first come, first served.
R Bar
This divey K-town bar is marked only with an "R" on the outside, but inside are great bands or karaoke and a good hang -- if you know the password.
How to get in: KNOW THE PASSWORD (which you can find on Facebook and Twitter).
No Vacancy
This's another Houston Brothers bar (told you they were the kings) with a similarly single-minded theme: the building used to be a brothel and a hotel, so you're transplanted into both, with almost Disney-style attention to detail -- and a death-defying, high-stepping show every hour on weekends.
How to get in: Get past the doorman, who'll show you to your room... and its bed.
Good Times at Davey Wayne's
This bar from the kings of the secret entrance (the Houston Brothers, it's the Houston Brothers), is a jaw-dropping, kind of hipster-y ode to the '70s, with DJs playing Fleetwood Mac, vintage beer cans, and hot chicks rollerblading.
How to get in: There's a garage, with one door. And it's the refrigerator.
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Viewed products
The Spanish means vineyard Vineyard...
The Spanish means vineyard Vineyard and AB refers to Andrés Botaina, the original owner of the vineyard the grapes. This vineyard was one of the first taken by Manuel Gonzalez after starting the wineries in 1840. He says that Lustau Amontillado Botaina also has a call, a diferent vineyard.
That's not very old for a amontillado. In fact it used to be labeled Amontillado Fino, indicating that it was a transitional style, but from 2009, this name could not be used anymore and simply was named Amontillado (while age remains the same) .In addition to being quite young, who is also on the light side: contains only 16.5% alcohol, which is not enriched again after losing his flower.
Warning: Last items in stock!
Availability date: 0000-00-00


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Last night I was woken up to 2 calls that my father's been vomiting up coffee ground like substance non stop. It's been 4 days since we sat together and he was mumbling about bicycles and cursing out mom's mom "that old woman person".
What is that feeling of navigating his dying in a home that barely cares for his body? Slowly the nurses move, unswerved from slow meanderings down hotel like hallways. Telling me they don't understand him well because he's not a native English speaker. No, what is clear about his unclarity is his dementia, his blood being coughed up. The lack of doctors, trained medical professionals who can observe his chart and his symptoms…and they're trying to put this on his English? It's an overwhelming feeling that still in the end, they pin incoherence on his accent over his actual illness and their lack of comprehension.
Or when sending him to the hospital at 3am, musing out loud he should go to hospice. The strong adherence to protocol and personal opinions…
When did care and attention to a person's dignity get so expensive?


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This is a limited edition dark gray variation of our geometric vase concrete planter. Only 1 available!
Hand-poured cement geometric vase.
This geometric vase is great for plants and also things that are not plants. Candles, succulents, cacti, pencils...really whatever your heart desires. It's a hole that you can put stuff in.
- Handcrafted in Alabama
- 4.2 x 4.2 x 4 inches
Note: Each item is individually hand-mixed, poured and painted. There will be slight variations in color, finish and surface texture.


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8105 47 Av Nw, Calgary
Welcome to this beautiful 2 storey home over 2700 sq ft developed on 3 levels.
Graceful living in this home flooded in natural light on main by huge windows, a west backyard and 6 skylights on 2nd floor. The chef's dream kitchen, exotic granite countertops & large island. The gas stove has 2 ovens, cabinets are fullsize to ceiling, pullouts in pantry cabinet and lots of drawers for well appointed kitchen. The family room with 3 sided fireplace viewable from kitchen nook to front entrance. Hardwood is an acacia wood which is throughout main floor, stairwell and upstairs. The master bedroom is expansive with sitting area, and well designed walk in closet. The master ensuite 5 piece tastefully finished in double sinks and large shower, soaker tub, won't disappoint. The loft which is currently a hobby room could be converted to a bedroom. Laundry room up also has an abundance of cabinets, granite counters. There are built in speakers on all 3 levels of home. Downstairs which was completed by builder has 10 ' ceiling, large recreation room, gas fireplace & wall unit, two large extra storage closets, generous furnace room with more storage. The 3rd bedroom with full bath, ideal for company or older child. All this situated in great location on quiet street, easy access to Downtown or Stoney trail. & close to Bowness Park for recreation for family. Worth your consideration call today.
Air Conditioning-Central, Ceiling 9 ft., Ceiling 10 ft., Deck, Insulation-Upgraded, No Animal Home, No Smoking Home, Patio, Programmable Thermostat, Skylight, Vaulted Ceiling, Wall Unit-Built-In, Vinyl Windows
Double Garage Detached, Insulated
Back Lane, Fenced, Flat Site, Low Maintenance Landscape, Park/Reserve, Playground Nearby, Public Transportation, Schools, Shopping Nearby
CREB® Data Ownership and Disclaimer Statement Data supplied by CREB®'s MLS® System. CREB® is the owner of the copyright in its MLS® System. The listing data displayed is deemed reliable but is not guaranteed accurate by CREB®.
The trademarks REALTOR®, REALTORS® and the REALTOR® logo are controlled by The Canadian Real Estate Association (CREA) and identify real estate professionals who are members of CREA. Used under license. The trademarks MLS®, Multiple Listing Service® and the associated logos are owned by The Canadian Real Estate Association (CREA) and identify the quality of services provided by real estate professionals who are members of CREA. Used under license.
Listing information last updated on April 4th, 2020 at 10:45pm CDT.


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Cholera is a contagious infection caused by
the bacteria Vibrio cholerae, which can in turn cause severe gastroenteritis and excessive
watery diarrhea for several days. Rapid dehydration and electrolyte imbalances
can be fatal as suspected in the deaths of James K. Polk, the 11th President of the United
States; and Charles the Tenth, King of France. V. cholerae is a gram-negative, curved bacteria
which looks like little red or pink comma-shapes on a gram stain. It's positive for oxidase and grows in alkaline
media. It has pili and a single polar flagellum,
kind of like a tail, at one end which it uses for movement through the gastrointestinal
tract. It's a facultative anaerobe so that means
it can undergo respiratory and fermentative metabolism. Transmission of V. cholerae typically occurs
through a fecal to oral route. This includes consuming untreated sewage water,
and anything that comes in contact with it, like raw or undercooked fish including shellfish;
and improper hygiene, like a lack of hand washing after a bowel movement. Cholera tends to be more common in developing
countries and places lacking advanced sanitation and sewage treatment facilities, with high
rates in some locations in Africa and South America. People who have low gastric acidity or have
an O-blood type are particularly at risk for a severe infection. Now, when V. cholerae enters the stomach it
shuts down protein production to conserve energy and nutrients, and to survive the acidic
environment. But once V. cholerae is in the intestines,
it uses its flagella to move toward the intestinal walls; propel through the mucous layer on
top of the epithelial cells lining the intestines; and attach to the finger-like cellular projections,
called villi, on the surface of the epithelial cells. There, V. cholerae can begin to multiply and
produce toxins. And though V. cholerae does not enter the
epithelial cells itself, the toxins do and they can cause a lot of trouble. Now, the exact toxins produced can depend
on the strain of V. cholerae. Some strains produce toxins that won't cause
any, or maybe just mild clinical symptoms. But some strains produce cholera enterotoxin,
also called choleragen, which is most often the cause significant clinical symptoms. When cholera enterotoxin enters the epithelial
cells, it leads to the ADP-ribosylation of the Gs alpha subunit of G-protein. So, this causes the G-protein to becomes permanently
activated and it keeps activating a membrane-bound protein called adenylate cyclase. This protein in turn leads to an overproduction
of the intracellular secondary messenger protein, cyclic adenosine monophosphate, or simply
cAMP. Increase in cAMP causes chloride channels
on the cells to increase the secretion of chloride into the lumen while inhibiting the
channels that let sodium and chloride back into the cell. So, the abnormally high levels of luminal
sodium and chloride disrupt the osmotic balance between the intestines and surrounding tissue;
and water, bicarbonate, and potassium rush into the lumen of the intestines as a result. This can cause symptoms like vomiting and
voluminous amounts of watery diarrhea containing extremely high concentrations of sodium, potassium,
chloride, bicarbonate; as well as high levels of live V. cholerae ready to infect another
individual. There could also be flecks of the mucous layer
of the intestine which looks similar to rice in water. Typically, there is no fever, pain or cramping. And while the incubation time for V. cholerae
can take hours, to 2-3 days; severe dehydration and depletion of electrolytes can happen within
4 to 12 hours of the first bout of diarrhea or vomiting. These imbalances can lead to symptoms like
disorientation, dry mouth, swollen tongue, sunken eyes, cold clammy skin, or shriveled
and dry hands and feet. Other, even more severe, fatal complications
can come from depleted electrolytes and water in the blood. Like low levels of bicarbonate can cause metabolic
acidosis with deep and labored breathing called Kussmaul breathing. Or low levels of potassium can cause muscle
dysfunction including leg cramps, weakness and abnormal heart rhythms. And low chloride and sodium can cause headaches,
poor balance, disorientation, seizures and coma. Finally, severe dehydration can cause hypovolemic
shock with a significant decrease in blood pressure. Diagnosis is typically made by a stool sample
growing V. cholerae on a thiosulfate-citrate-bile salts-sucrose agar. But even before a definitive diagnosis can
typically be made, the first line of treatment is to rapidly replace the lost water and electrolytes
with rehydration salts either orally or intravenously. In cases of mild to moderate diarrhea, this
type of supportive therapy is enough of a treatment course and the diarrhea may resolve
on its own in about 3 to 7 days. But more extreme cases may require antibiotics. In this case, the stool culture can be helpful
in deciding which antibiotics would be most effective against the infecting strain, and
may include tetracyclines, ciprofloxacin, ofloxacin, furazolidone, or trimethoprim-sulfamethoxazole. So, to recap: Cholera is a contagious, severe
gastroenteritis caused by the gram-negative Vibrio cholerae bacteria. It's route of transmission is fecal to oral
through consumption of contaminated sources like water and shellfish. Some strains produce cholera enterotoxin at
the surface of intestinal epithelial cells which leads to the over-activation of cAMP
by permanently activating Gs alpha. Patients most often present with voluminous
"rice-water" diarrhea that quickly leads to dehydration and electrolyte imbalances
that can be fatal if not rapidly treated with rehydration salts.
Vibrio cholerae (Cholera)
Cholera is a contagious infection caused by


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Academic Artist seeking to better understand life, culture, and religion through various rabbit holes
Friday, December 15, 2006
so frustrated...
ok so we had this nasty wind and rain storm last night and as of an hour ago when i left the house we still had no power. but this is not what i am frustrated about. i am frustrated with some of the men in my life. it is like there is this blank empty hole where consideration, reality(in one case), and honesty (self focused and external) should be. if it didn't effect me or other women i care very much for then i think i could just pass it off and let it go but it does.
i am tired of being lied to and manipulated, tired of watching two of my best friends being lied to and manipulated by self-centered men. this ignorance of what is happening is so incredibly stupid. why is it so hard for us to be honest? why do we say things just to be nice to the other person, especially someone you have a history with? women do all of this too i know that but right now it is men that i am frustrated with.
it just doesn't make sense to me to tell someone you care about them, even when time and time again they give you an out, and then treat them horribly. i guess the base of this entry is that i miss my friends i am tired of them hurting people who care about them or at least are trying inspite of what assholes they are.
i am also tired of peoples expectations. it is so much work to try and be what someone else think you should be....
PS- the picture is the cover of a cd i just got from the band Augustana...i saw them open for counting crows this summer and tucked their name in the back of my head. when i saw one of their videos on mtv last week at work i made sure to check them out again. they were by far the best opening act(that i hadn't heard of or was not there to see) i have EVER seen at a show. their set was incredible. listen to them they rock.
Posted by jessi knippel at 6:10 PM
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