text:
Peptides (v.26, #5)
IFC (editorial board) (CO2).
Anti-alopecia mechanisms of soymetide-4, an immunostimulating peptide derived from soy β-conglycinin by Takahiro Tsuruki; Kyoya Takahata; Masaaki Yoshikawa (707-711).
Previously, we found that orally administered soymetide-4 (MITL), an immunostimulating peptide derived from soybean β-conglycinin α′ subunit, suppressed alopecia induced by the anti-cancer drug etoposide in neonatal rats. Soymetide-4 has weak affinity for N-formyl-methionyl-leucyl-phenylalanine (fMLP) receptor. fMLP showed an anti-alopecia effect after intraperitoneal administration, though it was inactive after oral administration. Anti-alopecia effect of fMLP was blocked by pyrilamine or cimetidine, antagonists for histamine H1 or H2 receptor, respectively. However, the anti-alopecia effect of soymetide-4 was not inhibited by the histamine antagonists but by indomethacin, an inhibitor of cyclooxygenase (COX), or AH-23848B, an antagonist of the EP4 receptor for PGE2. Anti-alopecia effect of soymetide-4 was also blocked by pyrrolidine dithiocarbamate, an inhibitor of nuclear factor-κB (NF-κB). These results suggest that PGE2, which is produced after activation of COX by soymetide-4, might suppress apoptosis of hair matrix cells and etoposide-induced alopecia by activating NF-κB.
Keywords: Alopecia; Etoposide; Peptide; Prostaglandin; Soybean;
New antibacterial peptide derived from bovine hemoglobin by Rachid Daoud; Veronique Dubois; Loredana Bors-Dodita; Naima Nedjar-Arroume; Francois Krier; Nour-Eddine Chihib; Patrice Mary; Mostafa Kouach; Gilbert Briand; Didier Guillochon (713-719).
Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields an intermediate peptide fraction exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis after separation by reversed-phase HPLC. From this fraction a pure peptide was isolated and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). This peptide correspond to the 107–136 fragment of the α chain of bovine hemoglobin. The minimum inhibitory concentrations (MIC) towards the four strains and its hemolytic activity towards bovine erythrocytes were determined. A MIC of 38 μM was reported against L. innocua and 76 μM for other various bacterial species. This peptide had no hemolytic activity up to 380 μM concentration.
Keywords: Antibacterial peptide; Bovine hemoglobin; Pepsin; Active peptide; Hydrolysate;
Design and expression of peptide antibiotic hPAB-β as tandem multimers in Escherichia coli by Xiancai Rao; Jinchuan Hu; Shu Li; Xiaolin Jin; Chun Zhang; Yanguang Cong; Xiaomei Hu; Yinling Tan; Jianjun Huang; Zijin Chen; Junming Zhu; Fuquan Hu (721-729).
Peptide antibiotics are small peptides encoded by organism genomic DNA. They are recognized to play important roles in the innate host defense of most living organisms. The growing resistance of bacteria to conventional antibiotics and the need for discovery of new antibiotics have stimulated great interest in the development of peptide antibiotics as human therapeutics. However, preparation of peptide antibiotics at a large scale is a great challenge in developing these commercial products. In this study, tandem repeat multimers of peptide antibiotic hPAB-β were designed and the recombinant plasmids containing one to eight copies of hPAB-β gene were generated. Eight genetic engineered bacteria harboring pQE-hPAB-β1–8 recombinant were able to express the repetitive hPAB-β multimers of interest in inclusion bodies, respectively. The expressed proteins could reach 2.6–28% of the total proteins. The hPAB-β trimer construct was selected out for the subsequent study based on its higher expression level (27.8%), which yields in wet cell weights (3.15 ± 0.45 g/l) and the fusion protein inclusion bodies was able to completely dissolve in 8 M urea. The tandem trimers could easily be captured by Ni-NTA affinity chromatography and cleaved into monomers by hydroxylamine. Then, the monomer hPAB-β of interest was purified to 95% homogeneity by reverse phase chromatography and gel filtration. The final yield of purified recombinant monomer hPAB-β was 680 ± 12 mg/100 g wet cells. The minimum inhibitory concentrations (MICs) of the purified recombinant hPAB-β against type or clinical strains of microorganisms were about 31–250 μg/ml and these results showed that the recombinant hPAB-β could retain its bioactivity.
Keywords: Peptide antibiotic hPAB-β; Tandem repetitive multimers; Expression; Bioactivity;
Molecular cloning of a novel putative potassium channel-blocking neurotoxin from the venom of the North African scorpion, Androctonus amoreuxi by Tianbao Chen; Brian Walker; Mei Zhou; Chris Shaw (731-736).
Scorpion venoms are a particularly rich source of neurotoxic proteins/peptides that interact in a highly specific fashion with discrete subtypes of ion channels in excitable and non-excitable cells. Here we have employed a recently developed technique to effect molecular cloning and structural characterization of a novel putative potassium channel-blocking toxin from the same sample of venom from the North African scorpion, Androctonus amoreuxi. The deduced precursor open-reading frame is composed of 59 amino acid residues that consists of a signal peptide of approximately 22 amino acid residues followed by a mature toxin of 37 amino acid residues. The mature toxin contains two functionally important residues (Lys27 and Tyr36), constituting a functional dyad motif that may be critical for potassium channel-blocking activity that can be affirmed from structural homologs as occurring in the venoms from other species of Androctonus scorpions. Parallel proteomic/transcriptomic studies can thus be performed on the same scorpion venom sample without sacrifice of the donor animal.
Keywords: Scorpion; Venom; Neurotoxin; Potassium channel; Molecular cloning;
Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa by Ciaren Graham; Alexandra E. Irvine; Stephen McClean; Stephen C. Richter; Peter R. Flatt; Chris Shaw (737-743).
An octadecapeptide was isolated from the skin secretions of the dusky gopher frog (Rana sevosa) on the basis of histamine release from rat peritoneal mast cells. This peptide was purified to homogeneity by HPLC and found to have the following primary structure, YLKGCWTKSYPPKPCFSR, using both Edman degradation chemistry and peptide sequencing using high-resolution mass spectrometry (Q-TOF MS). The peptide, named peptide Tyrosine Arginine (pYR) shares 77.8% homology with peptide Leucine Arginine (pLR). The effects of the natural amidated peptide, non-amidated peptide and C-loop region of pYR on granulopoiesis and neutrophil apoptosis were investigated. All three analogues inhibited the early development of granulocyte macrophage colonies from bone marrow stem cells but did not induce apoptosis of the end stage granulocytes, the mature neutrophil. Thus, pYR is a novel member of an important and emerging new class of amphibian peptides with hemopoietic actions.
Keywords: Granulopoiesis; Histamine; HPLC; Peptidomics; Rana sevosa; Skin secretions;
Identification of cDNA coding for a homologue to mammalian leptin from pufferfish, Takifugu rubripes by Tadahide Kurokawa; Susumu Uji; Tohru Suzuki (745-750).
We identified cDNA coding for a homologue to mammalian leptin in puffer, Takifugu rubripes, using genomic synteny around the human leptin gene. In addition to significant sequence homologies, the puffer leptin (pLEP) displays characteristic structural features in common with mammalian leptin. The pLEP mRNA was expressed mostly in the liver that contained abundant lipids. In addition, homologues to pLEP were found in the databanks for three fish species (salmon, medaka, and Tetraodon) and two amphibians (salamander and Xenopus). The phylogenetic analysis shows rapid rates of molecular divergence among leptins from different vertebrate classes, but not between mammals and avians.
Keywords: Leptin; cDNA; Takifugu rubripes; Fish; Amphibian;
The role of previous exposure in the appetitive and consummatory effects of orexigenic neuropeptides by Stephen C. Benoit; Deborah J. Clegg; Stephen C. Woods; Randy J. Seeley (751-757).
The ingestion of foods is comprised of two distinct phases of behavior: appetitive and consummatory. While most food intake paradigms include both phases, the intraoral intake test emphasizes the stereotyped consummatory-phase by infusing a liquid food directly into the oral cavity. Several hypothalamic peptides have been shown to increase intake of chow in standard food intake paradigms and the current experiments sought to test whether these peptides would increase food intake in the intraoral intake paradigm. NPY, melanin-concentrating hormone (MCH) and orexin-A were infused into the third ventricle (i3vt) in a counterbalanced latin-square design just prior to rats getting 0.1 M sucrose solution infused via indwelling intraoral catheters and compared it to intake on bottle tests with access to the same sucrose solution. On the first day, each peptide increased intraoral intake relative to saline in the between-subjects comparison. Moreover, intake of sucrose following i3vt saline increased as a function of training. By the final day of the experiment, rats receiving saline consumed as much sucrose as rats receiving NPY, MCH, or orexin-A. This finding was conceptually replicated in the second experiment in which rats drank sucrose freely from a bottle on the home cage. A third experiment directly assessed the role of previous exposure in the sucrose intake induced by NPY. Those results confirm that repeated exposure to sucrose increases baseline intake and attenuates the hyperphagic effect of NPY. These results are consistent with two conclusions: (1) NPY, MCH, and orexin-A increase both appetitive and consummatory-phase ingestive behaviors on initial exposures; (2) repeated training interacts with the effects of these orexigenic peptides.
Keywords: Learning; Intraoral intake; MCH; Orexin-A;
Orexin-A-induced feeding is dependent on nitric oxide by Susan A. Farr; William A. Banks; Vijaya B. Kumar; John E. Morley (759-765).
Orexin-A is a peptide produced in the lateral hypothalamus/perifornical area, which stimulates feeding. The production of orexin-A is determined by the metabolic state of the animal. We have previously shown that nitric oxide (NO) plays an important role as a mediator of feeding induced by a variety of neuropeptides. This raises the question of whether orexin-A's effects are NO dependent. Here, we first determined that intracerebroventricular administration of 25 ng of orexin-A significantly increased food intake in satiated mice. We next examined the effects of N ω-nitro-l-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, on orexin-A-induced increase in food intake. L-NAME (50 mg/kg; SC) significantly blocked the orexin-A-induced increase in food intake. Orexin-A administration increased the levels of nitric oxide synthase in the hypothalamus. To further verify the importance of NO in the orexin-A-induced increase in food intake, we compared the ability of orexin-A to increase food intake in neuronal nitric oxide synthase knockout (NOS-KO) mice and their wild-type controls. Orexin-A failed to increase food intake in the NOS-KO mice, whereas it did increase food intake in the wild-type controls. This supports the hypothesis that nitric oxide is a central regulator of food consumption.
Keywords: Orexin-A; L-NAME; Mouse; Food intake; Nitric oxide; Orexigenic peptides; Hypothalamus;
Enhanced antinociception by intracerebroventricularly and intrathecally-administered orexin A and B (hypocretin-1 and -2) in mice by Jalal Izadi Mobarakeh; Kazuhiro Takahashi; Shinobu Sakurada; Seiji Nishino; Hiroyuki Watanabe; Motohisa Kato; Kazuhiko Yanai (767-777).
Orexins are neuropeptides located exclusively in neurons of the lateral hypothalamic area, which send projections to most monoaminergic nuclei, such as noradrenergic locus coeruleus, dopaminergic ventral tegmental areas, and histaminergic tuberomammillary nuclei. The present work was carried out to examine the role of orexins in nociception in mice. C57BL/6 mice were administered with orexin A and B intracerebroventricularly (i.c.v.), intrathecally (i.t.) and subcutaneously (s.c.) to reveal the sites of action of these peptides and to examine the pain thresholds using four kinds of nociceptive tasks. Orexins showed antinociceptive effects in all four types of assays for thermal (hot-plate, tail-flick, paw-withdrawal), mechanical (tail-pressure), chemical (formalin, capsaicin and abdominal stretch) nociceptions and nociceptin-induced behavioral responses, when administered i.c.v. or i.t., whereas the s.c. administration was ineffective. The antinociceptive effects of orexin A were more remarkable than those of orexin B. The i.c.v. administration of orexin A was as effective as, or more potent than the i.t. administration. The effects of orexin A were completely blocked by adenosine type 1 receptor antagonists, 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) and theophylline, but not by naloxone, suggesting a possible involvement of the adenosine-containing neurons and/or the adenosine pathway in these orexin actions. The i.c.v. administration of nociceptin had no significant effects on orexin expression in the brain and spinal cord. The present findings suggest that orexins have an antinociceptive role in at least four different types of pains, probably acting on both the brain and spinal cord.
Keywords: Orexin; Pain perception; Nociception; C57BL/6 mouse; Intracerebroventricular; Intrathecal;
Structural studies on 26RFa, a novel human RFamide-related peptide with orexigenic activity by Romain Thuau; Laure Guilhaudis; Isabelle Ségalas-Milazzo; Nicolas Chartrel; Hassan Oulyadi; Stéphane Boivin; Alain Fournier; Jérôme Leprince; Daniel Davoust; Hubert Vaudry (779-789).
A novel hypothalamic neuropeptide of the RFamide family, comprising 26 amino acids residues and thus termed 26RFa, has been recently characterized in human, and was found to be the endogenous ligand for the orphan G protein-coupled receptor GPR103. Intracerebroventricular injection of 26RFa provokes a robust increase in food intake in rodents. In the present study, we have investigated the solution conformation of 26RFa by using two-dimensional NMR spectroscopy in different media. In water, 26RFa exhibits mainly a random coil conformation although the presence of a nascent helix was detected between residues 6 and 15. In methanol, 26RFa adopts a well-defined conformation consisting of an amphipathic α-helical structure (Pro4–Arg17), flanked by two N- and C-terminal disordered regions. The strong conservation, from amphibians to mammals, of the amino acid sequence corresponding to the amphipathic helix and to the C-terminal flexible octapeptide of 26RFa, suggests that these two domains are crucial for the interaction of the peptide with its receptor.
Keywords: RFamide-related peptide; Neuropeptide; 1H NMR; Molecular modeling; Solution structure;
Neuropeptide FF receptors exert contractile activity via inhibition of nitric oxide release in the mouse distal colon by Quan Fang; Jia Guo; Min Chang; Li-xiang Chen; Qiang Chen; Rui Wang (791-797).
Neuropeptide FF (NPFF) and NPVF, two closely NPFF related peptides, have different affinities for the two NPFF receptors (NPFF1 and NPFF2). To assess the peripheral effects of NPFF receptors in the gastrointestinal tract motility, NPFF and NPVF were tested in the mouse isolated distal colon. Both NPFF (1–15 μM) and NPVF (1–15 μM) dose-dependently caused significant colonic contractions. Pre-treatment with the putative NPFF antagonist, BIBP3226 (30 μM) abolished the contractile responses to the two neuropeptides (3 μM). They had no additional contractile activities in colonic preparations contracted by N ω-nitro- l-arginine (30 μM). Moreover, the contractions of these two neuropeptides were weakened by l-arginine (2 mM). The responses to NPFF (5 μM) and NPVF (5 μM) were not modified by atropine or naloxone (1 μM). Furthermore, NPFF (1 μM) and NPVF (1 μM) did not influence the contractive responses to acetylcholine (0.1–10 μM), morphine (1 μM) or nociceptin (0.1 μM). These data suggest that NPFF and NPVF cause contractions of the mouse distal colon via their NPFF receptors and this effect is mediated by NO but not by cholinergic pathways, independently from opioid system. In addition, the isolated bioassay may be applied as a simple parameter to characterize the potential NPFF agonists and antagonists.
Keywords: Neuropeptide FF (NPFF); NPVF; Mouse distal colon; BIBP3226; Nitric oxide (NO); Opioid;
Substance P and the neurokinin-1 receptor in relation to eosinophilia in ulcerative colitis by Maria Jönsson; Örjan Norrgård; Sture Forsgren (799-814).
Substance P (SP) has been implicated in the pathophysiology of ulcerative colitis (UC) and it has been suggested that blocking of its effect would be advantageous in this disease. Eosinophils have also been implicated in the pathophysiology of UC. In the present study, specimens from the sigmoid colon of UC patients were investigated by the use of antisera against SP and the neurokinin-1 receptor (NK-1R) and staining for demonstration of eosinophils. The degrees of SP innervation and NK-1R immunoreaction, as well as the levels of eosinophil infiltration, varied between different patients. Interestingly, NK-1R immunoreaction in the epithelium was often seen to be the most marked where there were numerous eosinophils in the underlying mucosa and where the mucosa showed a marked morphologic derangement. The observations suggest that there are marked fluctuations in effects of SP and eosinophils during the disease. The infiltrating eosinophils may be involved in the destruction of the mucosal tissue. Furthermore, for the majority of cases where there is marked derangement of the mucosa, it is apparent that there is an upregulation of the NK-1 receptor in the epithelium in parallel with the infiltration of the eosinophils.
Keywords: Ulcerative colitis; Neuropeptides; Substance P; Neurokinin-1 receptor; Eosinophils;
Expression of galanin receptor messenger RNAs in different regions of the rat gastrointestinal tract by Laura Anselmi; Alexander Lakhter; Arlene A. Hirano; Marcello Tonini; Catia Sternini (815-819).
Galanin effects are mediated by three G-protein-coupled receptors: galanin receptor 1 (GalR1), GalR2 and GalR3. We quantified mRNA levels of GalR1, GalR2 and GalR3 in the rat stomach, small and large intestine using real-time RT-PCR. All three GalR mRNAs were detected throughout the gut at different levels. GalR1 and GalR2 mRNA levels were higher in the large than in the small intestine. GalR2 mRNA was most abundant in the stomach. GalR3 mRNA levels were generally quite low. The differential regional distribution of GalRs suggests that the complex effects of galanin in the gut are the result of activating multiple receptor subtypes, whose density, subtype and signaling vary along the gastrointestinal tract.
Keywords: Real-time RT-PCR; Intestine; Stomach; Enteric nervous system;
Dexamethasone reverses the memory impairment induced by antagonism of hippocampal gastrin-releasing peptide receptors by Roberta Venturella; Daniela Lessa; Tatiana Luft; Benno Roozendaal; Gilberto Schwartsmann; Rafael Roesler (821-825).
Storage of emotionally influenced memory is regulated by activation of glucocorticoid receptors (GRs) as well as of gastrin-releasing peptide receptors (GRPRs) in the dorsal hippocampus. In the present study, male Wistar rats were given a bilateral infusion of saline or the GRPR antagonist (D-Tpi6, Leu13 psi[CH2NH]-Leu14) bombesin (6-14) (RC-3095) (1.0 μg/side) into the dorsal hippocampus 10 min before training on an inhibitory avoidance task, followed by an immediate post-training i.p. injection of vehicle or the GR agonist dexamethasone (0.3 mg/kg). A retention test trial, carried out 24 h after training, indicated that intrahippocampal infusion of RC-3095 impaired inhibitory avoidance retention. Post-training administration of dexamethasone induced an enhancement of retention regardless of whether the animals had received saline or RC-3095 into the hippocampus before training. The findings indicate that hippocampal GRPR blockade does not prevent memory enhancement induced by dexamethasone. Together with previous results, these findings suggest that endogenous activation of GRPRs in the hippocampus modulates the consolidation of emotional memory, but is not a critical receptor system mediating memory formation.
Keywords: RC-3095; Bombesin-like peptides; Gastrin-releasing peptide receptor; Glucocorticoids; Hippocampus; Memory modulation;
Brain mast cells and therapeutic potential of vasoactive intestinal peptide in a Parkinson's disease model in rats: Brain microdialysis, behavior, and microscopy by Neşe Tunçel; Erol Şener; Cem Cerit; Umut Karasu; Firdevs Gürer; Varol Şahintürk; Cengiz Bayçu; Dilek Ak; Zeynep Filiz (827-836).
In the present study, the effect of systemically administered vasoactive intestinal peptide (VIP) (25 ng/kg i.p.) was investigated on drug-induced rotational behavior, extra-cellular dopamine levels and histology of corpus striatum in a 6-hydroxydopamine (6-OHDA)-induced rat model of Parkinson's disease. After 15 days of 6-OHDA lesion, apomorphine-induced (0.05 mg/kg s.c.) rotational behavior of the animals significantly increased and extra-cellular dopamine levels of corpus striatum were significantly reduced. VIP reversed the rotational deficits but did not alter the decrease in striatal dopamine levels. On the other hand, histological data indicate that VIP significantly reduced neuronal death and demyelination. Electron microscopic appearance of mast cells showed ultra-structural variety between VIP-treated and 6-OHDA lesioned groups. VIP activates mast cells without any evidence of typical exocytosis, and possibly mast cells could participate in neuroprotection. Our results suggest that systemically administered VIP can attenuate the motor response changes, neuronal cell death, and myelin sheet loss characteristically associated with 12 μg 6-OHDA administration into the rat striatum. Brain mast cells seem to participate in neuronal protection. Possibly, protective cues could be produced by brain mast cells.
Keywords: 6-OHDA toxicity; Corpus striatum; Dopamine; Rotational behavior;
The effects of neurotensin on selected parameters of lipid metabolism in rats by J. Piątek; H. Witmanowski; J. Paluszak; H. Krauss; J. Krawczyk (837-843).
15 nM/kg b.m. of neurotensin (NT) caused a significant inhibition of LMA within 30 min of administration and this effect persisted up for to the 240th minute of the experiment. A 15 nM/kg b.m. dose also caused a reduction in SLA which persisted up to the 120th minute. Sixty minutes after an intraperitoneal administration of NT a decrease in the cholesterol and NEFA levels and an increase in the TG and glycerol levels were observed. These effects were inhibited by the NTR2-blocker (levocabastine) and were not subject to change after an in vivo application of SR 48692.
Keywords: Neurotensin; Neurotensin receptors; Cholesterol; Lipid metabolism;
Effects of adipokines on expression of adrenomedullin and endothelin-1 in cultured vascular endothelial cells by Kazuhiro Takahashi; Kazuhito Totsune; Masahiko Sone; Kumi Kikuchi; Osamu Murakami (845-851).
Obesity is a major risk factor for the development of hypertension. Adipokines may cause hypertension by acting both centrally and directly on the vascular vessels. We wished to clarify whether three adipokines, leptin, resistin and tumor necrosis factor-α, affect expression of adrenomedullin and endothelin-1 in vascular endothelial cells. Human umbilical vein endothelial cells were cultured for 24 h with leptin (1–10 nmol/l), resistin (1–10 nmol/l) or tumor necrosis factor-α (1–10 ng/ml). Expression of adrenomedullin and endothelin-1 was examined by radioimmunoassay and northern blot analysis. Immunoreactive-adrenomedullin in the medium and adrenomedullin mRNA expression levels were decreased by treatment of tumor necrosis factor-α time- and dose-dependently, whereas endothelin-1 secretion was not significantly changed by it. Leptin or resistin had no significant effects on expression of adrenomedullin or endothelin-1 in human umbilical vein endothelial cells. Under hypoxic conditions (1% O2), expression of both adrenomedullin and endothelin-1 was induced in these cells. Immunoreactive-adrenomedullin levels in the medium were decreased by treatment of tumor necrosis factor-α under hypoxia. Leptin or resistin had no significant effects on adrenomedullin or endothelin-1 expression also in hypoxia. These findings have raised the possibility that decreased expression of adrenomedullin by tumor necrosis factor-α may be related to the increased risk of hypertension and other cardiovascular diseases in obese subjects.
Keywords: Adrenomedullin; Endothelin; Leptin; Resistin; Tumor necrosis factor; Adipokines; Obesity;
Carboxy-terminal PTH fragments stimulate [3H]thymidine incorporation in vascular endothelial cells by Ke-Hong Ding; Qing Zhong; Ding Xie; Jianrui Xu; Roni J. Bollag; Wendy B. Bollag; Carlos M. Isales (853-862).
We have previously reported that the intact PTH molecule (1–84) stimulates proliferation of human umbilical vein endothelial cells (HUVECs). To define the bioactive portion of the PTH molecule we utilized amino, mid and carboxy-terminal PTH fragments. Carboxy- but not amino-terminal fragments were equivalent to the intact PTH molecule in stimulating [3H]thymidine incorporation in HUVEC. Carboxy- but not amino-terminal PTH fragments increased intracellular calcium. Blocking the rise in intracellular calcium with calcium chelators abolished PTHs proliferative effect on HUVEC. In contrast to PTH 1–84, the carboxy-terminal fragment effect on [3H]thymidine incorporation was blocked by KN-93 an inhibitor of CaM kinase II. Taken together, these data suggest that the carboxy-terminal PTH is (or contains) the bioactive fragment responsible for the changes in intracellular calcium and thymidine incorporation in HUVEC stimulated with the intact PTH molecule.
Keywords: Endothelial cells; PTH; Proliferation; Signaling; Calcium; Receptors;
Ligand-dependent complex formation between the Angiotensin II receptor subtype AT2 and Na+/H+ exchanger NHE6 in mammalian cells by Lakshmi Pulakat; Shannon Cooper; Dieter Knowle; Chirag Mandavia; Steven Bruhl; Mary Hetrick; Nara Gavini (863-873).
Keywords: Angiotensin II; AT2 receptor; Na+/H+ exchanger; Human NHE6; Protein–protein interaction;
Solution conformation of Substance P antagonists—[d-Arg1, d-Trp7,9, Leu11]-SP, [d-Arg1, d-Pro2, d-Trp7,9, Leu11]-SP and [d-Pro2, d-Trp7,9]-SP by CD, NMR and MD simulations by Arati Prabhu; Alpeshkumar Malde; Evans Coutinho; Sudha Srivastava (875-885).
Substance P (SP) is an important neuropeptide involved in pain transmission and induction of inflammation. Its antagonists are being extensively investigated for their non-narcotic analgesic and anti-inflammatory activity. With a view towards better understanding the structural requirements of these analogs for efficient interaction with the SP receptor, the conformation of three SP antagonists [d-Arg1, d-Trp7,9, Leu11]-SP, [d-Arg1, d-Pro2, d-Trp7,9, Leu11]-SP and [d-Pro2, d-Trp7,9]-SP has been studied by CD, NMR and molecular dynamics (MD) simulations. All three peptides exhibit a high dependence of structure on the solvent. The molecules tend to adopt β-turns in solvents like DMSO and H2O and form helices in a hydrophobic environment. A direct relation between the helix forming potential of these antagonists with their receptor binding potency has been observed.
Keywords: SP peptide antagonist; 2d-NMR; Circular dichroism; Molecular dynamics;
Sauvagine analogs selective for corticotropin releasing factor 2 receptor: effect of substitutions at positions 35 and 39 on CRF2R selectivity by Adam W. Mazur; Feng Wang; Michelle Tscheiner; Elizabeth Donnelly; Robert J. Isfort (887-891).
Corticotropin releasing factor 2 receptor selective analogs of the amphibian peptide sauvagine, a member of the corticotropin releasing factor (CRF) peptide family, have therapeutic potential for the treatment of skeletal muscle atrophy. Previously, we demonstrated that [P11X12X13]Svg peptides have improved CRF2R selectivity, although not to the level of CRF2R selective hormones such as urocortin 2 and urocortin 3. Since we also demonstrated a potential for improvement in selectivity of sauvagine by modifications of residues 35 and 39, we investigated substitutions of these amino acids in selected [P11X12X13]Svg peptides. We have observed that substitution of Arg35 in sauvagine to Ala35 (the amino acid found in all CRF2R selective agonists), increased the selectivity of [P11, X12, X13]Svg analogs. In contrast, substitution of Asp39 in sauvagine to Ala39 (also the amino acid found in all CRF2R selective agonists) did not further increase the selectivity of [P11, X12, X13, A35]Svg analogs. Thus, the residues 35 along with 11, 12, and 13 in sauvagine represent important sites for improving CRF2R selectivity.
Keywords: Sauvagine; Corticotropin releasing factor receptor; Skeletal muscle atrophy;
Glutamate antagonists attenuate the action of NC-1900, a vasopressin fragment analog, on passive avoidance task performance in mice by Tomoaki Sato; Takayuki Ishida; Koh-ichi Tanaka; Nafees Chowdhurry; Masahiro Irifune; Kenji Hirate; Tamotsu Mimura; Takashige Nishikawa (893-897).
To examine the relationship between glutamate receptors and the action of NC-1900 on a step-through passive avoidance (PA) task in mice, MK-801, an NMDA receptor blocker, and (S)-4-carboxyphenylglycine (4CPG), a group I metabotropic receptor antagonist, were administered intraventricularly (i.c.v.) singly or as co-injections. The i.c.v. injection of MK-801 (0.8 μg) or 4CPG (2 μg) decreased the latency on the PA task. NC-1900 (1 ng/kg, subcutaneously (s.c.)) alone prolonged the latency on the retention trial in the PA task. MK-801 (0.2 and 0.8 μg) or 4CPG (0.5 and 2 μg) significantly inhibited the action of NC-1900, while the s.c. injection of NC-1900 did not affect latency in mice that received i.c.v. co-injection of MK-801 and 4CPG at any of the doses tested. These results suggest that glutamate receptors participate in the action of NC-1900 on learning and memory in PA task performance.
Keywords: NC-1900; Memory; MK-801; 4CPG; Glutamate receptor; Vasopressin analog;


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Customer Data Platforms (CDP) Market Outlook, 2019-2024 - Trends, Forecasts, Competitive Analysis, SPARK Matrix - Quadrant Knowledge Solutions
13 May, 2019, 13:00 BST
MIDDLETON, Massachusetts, May 13, 2019 /PRNewswire/ -- Quadrant Knowledge Solutions announces the addition of the "Market Outlook: Customer Data Platforms (CDP), 2019-2024, Worldwide" report to their strategic market outlook offerings. Customer Data Platforms (CDP) helps marketers to implement data-driven marketing initiatives to improve customer engagement, conversation rate, and overall customer experience.
For more information, visit: https://quadrant-solutions.com/market-research/market-outlook-customer-data-platforms-cdp-2019-2024-worldwide/
Organizations have invested in multiple marketing and data warehouse systems over the years, and most of these are often in silos. These systems may provide a capability for centralized data integration and customer-level data for various marketing use-cases. However, the customer data unification at an individual-level remains a challenge. Marketers continue to spend significant time and resources in data integration, preparation, and unification project. Additionally, ever-growing complexities, volume and speed of customer data across consumer interaction channels are further adding to the challenges.
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CDP platform integrates data from first-party, second-party, and third-party sources, such as website, web forms, social media, emails, e-commerce channels, CRM, data management platforms (DMP), POS systems, and such others. It integrates a variety of customer data types includes personal, demographic, behavioural, transactional, engagement, device data, and such others.
Quadrant Knowledge Solutions Customer Data Platforms (CDP) Market Outlook research provides strategic information to the market participants and users who are responsible for strategic planning, marketing, sales, and purchasing CDP solutions. This research provides strategic information for technology vendors to better understand the market supporting their growth strategies and for users to evaluate different vendor capabilities, competitive differentiation, and their market position.
SPARKTM Matrix: Quadrant Knowledge Solutions' SPARK Matrix (Strategic Performance Assessment and Ranking) provides a visual representation and strategic insights on how each vendor ranks against their competitors, for various performance parameters based on the category of technology excellence and customer impact.
CDP Market Outlook study includes analysis of key CDP vendors including ActionIQ, AgilOne, Amperity, BlueConic, BlueVenn, D4t4 Solutions, Evergage, Lattice Engines, Lytics, mParticle, NGData, Quaero, Red Point, RedEye, SessionM, Tapad, Tealium, and Zylotech
Research Contents
Executive Overview
- Customer Data Platforms Market Overview
- Key Research Findings
Market Background, Key Market Trends, and Market Drivers
Market Adoption and Deployment Trends
Market Forecast Analysis by Worldwide Regions
- Americas
- Asia Pacific
Market Forecast Analysis by Industry Verticals
- Banking, Financial Services, & Insurance (BFSI)
- Entertainment & Sports
- Healthcare
- Hospitality & Travel
- Media & Publishing
- Retail & eCommerce
- Telecom
- Others
Competition Landscape & Analysis
- Competition Analysis
- Competitive Factors and Technology Differentiators
- Leading Vendor Profiles and Analysis
- SPARK Matrix: Customer Data Platforms (CDP)
Research Methodologies
Keywords: Customer Data Platforms, CDP Market, Customer Analytics, Customer Intelligence, Identity Unification, Identity Resolution, Customer Segmentation, Unified Customer Profile, Customer Data Management, Advanced Analytics, Machine Learning, Artificial Intelligence, Customer Experience, Customer Acquisition, Customer Retention, SPARK Matrix, Market Research, Market Size, Competitive Analysis, Market Forecast, Market Size, Technology Trends
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Quadrant Knowledge Solutions is a global advisory and consulting firm focused on helping clients in achieving business transformation goals with Strategic research, consulting, and advisory services.
Riya Mehar
See available research: https://quadrant-solutions.com/market-research/
SOURCE Quadrant Knowledge Solutions

subset: commoncrawl


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Sunny & Co Toys GS2820 Sunny Toys GS2820 28 In. Shakespeare, Sculpted Face Puppet
by Sunny & Co Toys
by Sunny & Co Toys
These puppets are in from top of head to tip of toes and can accommodate adult-size hands. Their large size makes them especially good for ventriloquist use before large audiences. All of these puppets have sculptured faces and finely detailed clothing. Hand goes into back of puppet and up into head to work mouth.Features. Sculpted Face Puppet - Shakespeare. Size - 28 in.. Item Weight - 2 lbs.
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It's time for our current students to submit their intent to return for the 2017-2018 school year.
Some of you are familiar with the process, but we also have several new parents who will be participating for the first time with this process.
Here is how the process works:
- The intent-to-re-enroll form was distributed on Wednesday, February 8, 2017 and must be returned by Wednesday, February 15, 2017
- Each Student must return a form rather he/she is returning for the 2017-2018 school year
If you have any questions, please contact Ms. Jones, the Director of Admissions and Student Support Services at 215.476.6471 x112.


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KENDUSKEAG - All the signs are here. The weather is warming up, the snow is disappearing and baseball is being played. Yes, spring has sprung, and one of our area's most beloved traditions is upon us again.
It's Kenduskeag Stream Canoe Race time.
Hundreds of hopefuls will once more descend on the town of Kenduskeag on April 15 for the 51st annual running of the Kenduskeag Stream Canoe Race. The race will commence at 8:30 a.m.
A little history: The first race was held in 1967, and the Bangor Parks and Recreation Department has been involved since its inception. In the entire history of the race, there has never been anything in the way of corporate sponsorship. It is a wonderfully self-contained community event.
The first race had 34 paddlers participating. The number of racers has varied from year to year, with peak numbers as high as 1500 brave boaters during the mid-1990s. Over the years, tens of thousands of people have taken part in this iconic event.
It's worth noting that the Kenduskeag Stream Canoe Race is the largest paddling event in New England and one of the largest in the entire country.
The race course is 16.5 miles long, with 10 miles of flat water. The other 6.5 miles consist of rapids of varying degrees of difficulty, from class I through class III. Generally, the most difficult area for paddlers to negotiate is Six Mile Falls. Fortunately for spectators, there is a bridge right there, providing a first-rate vantage point to enjoy the inevitable spectacular splashdowns. This is where the prime tips and flips really happen.
There are a wide variety of competition classes; over 20 in all. There are different categories for kayaks and canoes, one or two paddlers. There's even an "open" class, consisting of boats carrying more than two people and any craft that doesn't necessarily qualify as either a canoe or a kayak. The classes are also broken down by level of experience. Essentially, this means that no matter where on the spectrum your paddling skills fall, there's a place for you in the race.
Registering for the race is easy and can be done in a number of different ways. You can download the registration form from the Bangor Parks and Recreation website and mail it in along with your registration fee. You can register and pay online (though you'll still need to sign and deliver the form to Parks and Rec). You can also register in person at the Parks and Recreation office right up until 1 p.m. on April 14. Finally, you can register onsite on race day from 6:30 to 7:45 a.m.
Preregistration is recommended; signing up early means a fee of $25 per paddler versus $50 apiece on the day of the race.
The Kenduskeag Stream Canoe Race is one of the most entrenched and entertaining traditions that this area has to offer. Whether you participate or just go to watch, it's a heck of a good time. And trust me, anyone can do it. How do I know?
Because I've done it. Twice.
Memories of races past
I have a longstanding connection to the Kenduskeag Stream Canoe Race. I grew up in Levant – West Levant, to be precise.
This meant that the canoe race was nearby. You could hop into a car at my house, make a right turn out of the driveway and in five minutes, you're at the bridge where the race launches. Heck, there were a couple of times in my youth that I straight up rode my bike over there to watch some of the racers head out.
And yet I never actually PARTICIPATED in the race when I was young. I had friends and relatives who would do it, but for whatever reason, I never got into a canoe myself while I was living practically next door to the thing.
It took the transition to adulthood for me to reach the point where I would actually participate in the race. As of this writing, I have paddled in the Kenduskeag Stream Canoe Race twice in my life – once in 2001 and again in 2008 – and while I'd like to take one more go at it, I'm rational enough to recognize that the combination of my inherent laziness and the unstoppable march of time likely means that it's "Two and Through" for me and the race.
Care to float down Memory Stream with me?
In April of 2001, I was living in a house on French Island in Old Town with a bunch of degenerates (though it should be noted, three of those degenerates – one of whom features prominently in this story – were in my wedding party; all of us somehow became upstanding citizens when we weren't paying attention, but that's a story for another time).
My friends Nick (who would serve as my best man slightly more than a decade later) and Steve – outdoorsy types both – had decided to paddle the race and asked me if I would be interested in joining them. Having grown up nearby but never participated, I agreed.
We borrowed a canoe from Nick's parents the night before the race and made our way to the starting area that morning. Nick and Steve were dressed appropriately while I was clad in flannel and jeans and basically everything that they recommend against wearing. Nick and Steve prepared the boat with all the inflatable doodads and whatnot while I stood around being generally useless, as was (and still is) my way.
And yet, I never got the sense that they regretted asking me. Fools.
So it came time to start. Our initial hint that things might be a bit tricky for us was when we all three got into the canoe for the first time and pushed off into the water – Nick at the front, Steve at the back, me in the middle. There was a lot of beef in that boat; we were sitting unexpectedly low in the water.
As we tried to maneuver ourselves into position, my inexperience became very clear. Now, I had been very upfront about my canoeing abilities or lack thereof; I told them that I could count on one hand the number of times I had ever even been in a canoe. But despite my flailing ignorance, my boatmates were optimistic.
Then we started rowing.
Those initial 10 miles of flat water are exhausting to race through – particularly when you've got a boat weighed down in the middle by a heavy dude who doesn't really know how to paddle. We pushed our way through it, but just before we went round the bend to approach Six Mile Falls, Nick suggested a pit stop.
See, it turns out that Nick's grandparents lived right there on the banks of the stream. So we decided to get out, stretch our legs, have a snack and so on. As you've probably figured out by now, the "race" part of the canoe race wasn't really a big concern for us. After 45 minutes or so, we got back into the water and headed for the Falls.
And then almost immediately went into the drink.
Heavy boat, you see – we wound up getting stuck on rocks in the middle of the falls and tipping over, much to the delight of the vultures. We drifted under the bridge, climbed out and dried out. As an added bonus, I had lost my paddle, so not only would I be weighing us down for the rest of the trip, I couldn't even contribute.
So I sat in the middle and had a leisurely cruise the rest of the way while Nick and Steve propelled us toward the Penobscot. A highlight was going through downtown and realizing that due to the high water, we were actually going to have to lie down to get under one of the footbridges.
And that was that. If you've ever wondered what kind of people wind up finishing last in the Kenduskeag Stream Canoe Race, well … now you know.
In 2008, I had been part of the team here at The Maine Edge for a while. One day, I pitched the idea of doing a story where I participated in the 42nd running of the Kenduskeag Stream Canoe Race. Fellow Edge staffer Pat Shaw volunteered to join me, so we registered for the race. Our canoe was a medium-sized green number courtesy of Mike Fern. Our registration number was 233.
I hadn't been in a canoe since my previous go-round with the race; Pat's paddling experience wasn't much deeper. Still, when we set out that morning, things were looking good. We managed to get into the water without embarrassing ourselves, and soon we were making our way through the ten miles of flat water that leads to the excitement of Six Mile Falls. After some initial difficulties with regards to steering, there was steady progress.
However, our journey truly began when we came upon the falls. We followed a group of our fellow racers into the beginning stages of the falls. It rapidly became clear that this was a mistake. There was a bit of a traffic jam that resulted in our canoe getting bumped, spinning sideways, and finally tipping us into the stream.
Maine streams in April are pretty chilly, in case you were wondering.
Undaunted, we pulled ourselves and the canoe out of the stream, dumped the water out of the boat, and set out to hit the really difficult part of the falls in earnest. We emerged into view of hundreds of spectators, most of whom would like nothing more than to see us get dumped by the rapids (our previous fall had gone unseen by most observers). We hit the falls; Pat made eye contact with a spotter who seemed to think that we were about to get wet (again). Instead, we went through dead center, over a drop of three feet or so, and managed to stay upright. We even heard a few cheers from the bridge and banks.
There was no time for celebration, however, as there was still plenty of stream ahead of us. Still, we were feeling pretty good about ourselves. We had made up some time through the falls, and were now moving at a nice clip. We breezed through the first mandatory portage with no problems, and things were looking up.
This is where we may have gotten a little cocky.
We kept on down the stream; then, the current started picking up. We were rapidly descending on the second portage. Unfortunately, we couldn't see the sign, and by the time we realized what was happening, we had been spilled once more into the frigid waters of the Kenduskeag, losing one of our paddles. We were rescued by one of the many wonderful safety personnel, but despite our best efforts, our canoe had continued downstream, getting stuck mid-stream atop another canoe that was in turn stuck on a rock.
Eventually, race officials dislodged the canoe, sending it downstream. We took off after it; Pat proceeded to run to the water, jump in, and swim to the canoe. After a struggle, he snagged it and managed to make his way to shore. After that ordeal, however, we decided that it wasn't to be, and informed a nearby official that we were out of the running.
So we didn't make it to the finish line. Regrettable, but it doesn't change the fact that we made a (kind of) fine showing and did the Edge (sort of) proud.
In the end, I greatly enjoyed my canoe racing adventures, which just goes to show that even those of us who are less disposed to "being outside" and "doing things" can have a good time hitting the water and heading downstream. The Kenduskeag Stream Canoe Race is one of those uniquely Maine events that everybody should try at least once. Take part in the tradition and have some fun.
And try to stay dry.


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Thursday, July 12, 2007
Finally - Xcaret!
We did make it to Xcaret - we actually went the day before going to Isla Mujeres. It is an incredible place - lots to do and amazing stuff to see - animals, swimming, maya village, butterfly garden. We don't have pictures of this but we all snorkeled in an underground river. It was kind of cold and a little awkward for the boys, but Bekah did an incredible job with her snorkel gear. I was impressed. Another highlight is the show they put on in the evening - really a first class presentation of Maya culture, Mexican culture, and traditional dances. Sadly, our batteries died out during the performance, but we enjoyed a ball game - Maya style (you know with the hoop on the wall like you see in Chichen Itza), tradiational dances from different regions in Mexico (one called Viajitos or in other words, "senor citizens" where all the male dancers wear masks depicting old men - then at the end they reveal that they actually are in the elders category!), and men hanging from ropes at the top of a huge pole and spinning. Ok, I'm done, I'm not doing the show justice, but you can see some professional shots here.
There wasn't nearly enough time to see and do everything - going with a group kind of lent itself to that, but we really enjoyed what we were able to experience right down to the buffet dinner "included" in our ticket price ;-)
The place costs and arm and a leg, but if we ever save up to go there again . . . we'll get there when the doors open and stay til they kick us out. Enjoy the few shots we did manage to take on the slideshow or check out the photos on this website.
Back to work now . . .
Posted by Unknown at 4:45 PM 1 comment: Links to this post
Labels: Vacation Cancun
Thursday, July 05, 2007
Isla de Mujeres
The Friday of our Cancun vacation, we "chose our own adventure." Heading out the door of our condo with a picnic lunch, we drove to Puerto Juarez, parked the car and hopped on a ferry traveling to "women island" (I like the name better in Spanish . . .). Once there, we rented a golf cart and explored the island on a gorgeous day - the last of May. As we explored, the kids fed sea turtles, touched a shark, picked up a starfish and shell souvenir, enjoyed a picnic lunch, and swam in the ocean. Later, we enjoyed seafood at a local restaurant and splurged afterwards on ice cream - yummy! The trip back on the ferry was incredible as we took in the beautiful colors of the sunset and felt the ocean breeze on our faces.
Posted by Unknown at 5:24 PM No comments: Links to this post
Labels: Kids, Vacation Cancun
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It's cold outside. Let's just make a bowl of pasta and be done with it. This recipe is pretty easy and super tasty, you could use cheddar if you don't have parmesan and I can use any mushrooms you like. You don't have to make the breadcrumbs but I really like adding a bit of … Continue reading Mushroom and sage tagliatelle with garlic breadcrumbs
Tag: vegetarian
Spicy bean and cheese wraps
Honestly, a decent packed lunch is what gets me through long days of studying. As much as I love a Tesco meal deal, it's a lot cheaper (and nicer) to make your own lunch. This recipe is pretty quick and easy and involves no cooking and very little washing up - yay! If you'd like … Continue reading Spicy bean and cheese wraps
Butter bean, squash and tomato salad
I'm a big fan of packed lunches you only have to make once. I'll make a salad like this one on a Sunday night and not have to think about making a packed lunch for the next 5 days - dreamy! There's a nice combination of summer and autumn in this recipe too - fresh … Continue reading Butter bean, squash and tomato salad
Mushroom bolognese
This recipe is a super quick and easy alternative to the usual beefy bolognese. You can use whatever mushrooms you can get your hands on, or you could even use lentils and dried basil for a total storecupboard meal. If you're lacking ideas I've also added some extra recipes at the end for some bolognese-y … Continue reading Mushroom bolognese
Sunshine pasta: Tomato, ricotta and basil macaroni
My sunshine pasta is exactly the kind of thing I like to cook, midweek, when there's still some sun lingering on from the day, but it's getting a bit nippy outside. The sweet tomatoes complement the creamy ricotta and little bites of basil very nicely too. It's safe to say I think I've found my … Continue reading Sunshine pasta: Tomato, ricotta and basil macaroni
Spaghetti with cavolo nero, chestnuts and ricotta
This is the kind of dinner you can knock up in about 15 minutes - it's really easy and, most importantly, delicious. It's also a nice way to eat more greens - which can prove difficult when it's cold and all you want to eat is potatoes! I love the ricotta in this recipe, but … Continue reading Spaghetti with cavolo nero, chestnuts and ricotta
Clementine Drizzle Cake
Clementines really are at their best at this time of year. This is exactly why I couldn't bring myself to throw away some slightly squishy little fruits in our fruit bowl that hadn't quite made the mark. Instead of going in the bin, they've been repurposed in the form of this tasty clementine cake with … Continue reading Clementine Drizzle Cake


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Two assets I made for "Adventures of Pip" by Tic Toc Games. I made more stuff but these 2 are my favourites. There is not much optimisation going on in terms of colours, because hey, work stuff and no limitations.
Adventures of Pip (c) Tic Toc Games
Love it. So much detail crammed into such a small picture though! I think it would look better more spread out! But.. that's just me!
- Looks really cool. -


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Host, Ted White interviews formerly incarcerated guests and they tell how the Gospel has changed their lives. Also, hear from guests who volunteer and who minister in New Hampshire's correctional institutions.
Air Times:
WVNH 91.1 and WANH 88.3 – Sundays @ 7:00 PM
Listen here: https://soundcloud.com/user-899071514
Concord TV – Public Channel 22, Sundays @ 06:30 PM and several times throughout the week
Watch Live and On-Demand episodes of Prison Chapel of the Air at this link:


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Remembering The Invergowrie Foundation in your Will
By including The Invergowrie Foundation in your Will you are supporting the work of the Foundation to advance the education of girls and women in Victoria.
The Foundation awards grants that will make a significant impact on the education of girls and women in Victoria, enabling access, promoting excellence, advancing leadership and supporting research.
The Foundation is committed to long term viability and steady growth in its corpus to increase its philanthropy. Since the Foundation's establishment in 1992, growth of the corpus has enabled grants and scholarships in excess of $8.0 million to have been awarded.
Your gift would help the Foundation to continue making a positive difference in the lives of girls and women in Victoria.
You can include The Invergowrie Foundation in your Will when you first have your Will drawn up or by adding a codicil to an existing Will. A gift can be for a specific purpose or to be used at the discretion of the Trustees.
Please note that the correct legal entity is The Trustee for The Invergowrie Foundation.
The Chief Executive Officer of The Invergowrie Foundation would be pleased to help you with information about the ways that you can assist the Foundation through a bequest. Alternatively, an appointment can be arranged with the Chairperson of The Invergowrie Foundation to discuss how your bequest would be applied to help advance the education of girls and women in Victoria. You are most welcome to telephone or write to the Chief Executive Officer at the following address, or simply return the completed reply slip.
The Invergowrie Foundation
First Floor
24 Glenferrie Road
Malvern 3144
E: firstname.lastname@example.com
If you decide to leave a gift in your Will to The Invergowrie Foundation please let us know as we would appreciate the opportunity to thank you.


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I have to admit here, I consider Shunji Iwai one of the most originals voices of Japanese cinema, an artist whose every work deserves more than a look, to say the least. "A Bride for Rip Van Winkle" is definitely among his best works, and this says a lot for the director of films like "Picnic", "All About Lily Chou Chou" and "Hana and Alice".
Buy This Title
The three-hour script is split in three parts and revolves around Nanami, a timid, extremely low profile woman, who, at the beginning of the movie, works as a part-time teacher, despite having issues with her students who mock her for her very low voice, and in a convenience store, to make ends meet. She spends most of her free time on "Planet" a social network, and seems to have no actual friends or a boyfriend. However, eventually she takes part in a blind date with Tetsuya, after meeting him online, who is also a teacher. The two of them get along from the beginning, and soon decide to get married. However, Tetsuya proves to be a mama's boy, and additionally, asks from Nanami, who has no friends and her parents are divorced, to find additional people to invite to the wedding. Having no alternative, Nanami turns to Amuro, a mysterious jack-of-all-trades who provides guests for weddings among his other services.
Amuro seems to be much more, though, as (in the second and third act) he becomes an important factor in Nanami's life, as he also helps her when she thinks her husband cheats on her and when her marriage collapses. Additionally, he finds odd jobs for her to help her financially, first as a housekeeper at a cheap hotel, then in a supposedly vacant mansion, and somewhere between, as a paid wedding guest. During the last, she meets Mashiro, the Rip Van Winkle of the title, and the two of them connect intensely, as their mutual feelings of loneliness collide, during the third act. However, it is soon revealed that neither Mashiro nor Amuro nor the house is what Nanami thinks.
Iwai manages to present a number of messages regarding Japanese society, through a relatively abstract narrative that goes in and out of surrealism a number of times. The lack of actual communication among people and the "solution" provided the social media depict one of the most central, with the consequences of actually making life decisions based on people one meets online being disastrous. The fact that money seems to buy everything, from guests at a wedding to actual friends, is another, with Iwai including an ironically funny sequence where Amuro is taking care of a number of children in the park, actually having them on leashes, as if they were animals. The relationships of parents and children, which stretch from almost non-existant to oedipal is another one, while the futility of the concept of marriage, which derives from silly social rules and constant pretentiousness is another.
All of the above come together in the last act, with the relationship that is formed between the two girls including almost every aforementioned comment, in rather surrealistic fashion.
In this setting, the roles of the three protagonists seem almost archetypical. Nanami does not know who she is (even if she is actually something) nor what she wants, as she floats in life, always ready to be directed to wherever, by the people around her. Haru Kuroki does a great job of portraying the character, with a very fitting timidness that never changes.
Amuro seems to know what he is doing, but he is also incomplete, as his life gives him little time for actual, personal relations, while everyone in his life seems to be a client, in extreme, capitalistic fashion. He seems to be able to get his way no matter what the circumstances, but even this trait does not lead him anywhere. Go Ayano is impressive in this part, always appearing cool and in control, and ready to go to extremes to achieve his goals.
Mashiro is the one who is willing to go to extremes to achieve human connection, and a truly sad character, whose riches does not seem to able to change her fate. The short-lived time of happiness she has with Nanami is actually completely demolished as the film progresses and her true character is revealed, including her relationship with her mother, in one of the strongest scenes in the movie. Cocco does an excellent job of portraying her, managing to emit fake happiness and sadness, at the same time, while her chemistry with Kuroki, which derives from their character's differences, is one of the film's highlights.
At times, the three characters reminded me much of the ones in Sion Sono's "Guilty of Romance", which seems to have influenced Iwai, in a number of corresponding concepts, although without the violence and the sex that dominate Sono's movie.
Technically, the film is more than impressive, with Chigi Kambe's cinematography portraying the kaleidoscopic nature of the narrative in impressive fashion, and in a plethora of various locations. The scene with the hidden cam, and the sequence in the fish room where a blue tint dominates the image, are among the highlights of an outstanding effort. Shunji Iwai's own editing induces the film with the sense of surrealism, in a hypnotic; almost dreamy style that fits its aesthetics quite nicely, although, at times, I felt that some scenes could have been avoided or even cut short, at least in the 179 minute version. Kyoko Heya's art direction is also among the production's highlights, with the majority of settings looking impressive and meaningful at the same time.
"A Bride for Rip Van Winkle" is a truly great film, one of the best in Shunji Iwai's filmography and a great sample of meaningful, contemporary Japanese cinema.


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Young Carers Awareness Day this year focuses on mental health and the support that young people need in their caring role.
A young carer is a person under 18 who provides or intends to provide care for another person. The number of child carers has continued to rise in the UK and these carers typically spend between 20 to 50 hours a week on caring duties. In 2017 it was estimated that there are over 700,000 child carers in the UK. The Children and Families Act 2014 gives young carers the same right to an assessment as adult carers, including the appropriateness of the child providing care in light of their own needs.
It is especially difficult for those who have not had the responsibility of caring for someone to imagine what it is like for someone to undertake this on a daily basis, often under conditions that are economically and socially strained and restrictive. The burden felt by young carers can be immense, and if there is little emotional support, it can overwhelm them.
Produced by Little Fish Films, Gus Filgate's film Pie tells the story of a young carer's determination to treat his granny like she used to treat him. The film goes a long way to helping people understand the emotional investment and choices that young people make to make the lives of their loved ones better.


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The braincase of a skull may well be,as advertised,a strongly built and cleverly engineered structure,but listening to all that incessant banging coming from the direction of the crab apple tree in the garden,one has to wonder: Is it really strong enough to keep a woodpecker from having the most terrible headache?
And what about those rams you see butting heads with such determined ferocity? The crashing sounds,the visions of extreme violence,the frequent tangling of hornshow do animals with an instinctive need for such brutish behaviour prevent their brains from turning into rice pudding?
Some answers are to be found in the upstairs bedroom of a decidedly unremarkable suburban house on the outskirts of the city of Coventry,in the English Midlands. This is where a collector named Alan Dudley has spent the last four decades bent to a curious obsession. By day he works selecting veneers for gluing to the dashboards of expensive automobiles. After work,he collects skulls.
He now has thousands,from the great hulk of a hippopotamus skull to the tiniest and most delicate tissue-like skull of a wren,and his collection makes up one of the finest and most comprehensive known. Included in it are many skulls of creatures that do strangely violent things to their headsthe ram and the woodpecker among them.
Both creatures happen to have very dense skulls,especially in that rounded rear area known as the braincase,where they are built like armoured cars. Crucially,their braincases are also unusually smooth inside.
The brains of most animals that are prone to head banging these include deer and other antlered mammals,as well as various birdsare relatively small and smooth-surfaced; and theyre bathed in only small amounts of cerebrospinal fluid,leaving little room for the brain to move and be shocked by the sudden decelerations and accelerations of their weaponised heads.
Moreover,both rams and woodpeckers are scrupulous in the precise fashion in which they smash their heads into things,whether trees or one another: The aim is such that theres very little side-to-side torsion exerted on the brain,none of the movement that induces whiplash injury and other kinds of damage.
Gannets have solved a similar problem. These magnificent seabirds,with wingspans of as much as 6 feet,catch fish by spectacular dives into the ocean. Starting from heights of 100 feet or more,they enter the water at 60 mph and hurtle downward far beneath the surface,pursuing their chosen fish underwater using their wings to swim.
It's an awesome performance not least because they are so successful as hunters: They are eagle-eyed and they have true binocular vision,which helps them lock on target. However,their fishing success is one thing. Their survival is quite another. To dive into water from 100 feet may not be lethal for a gannet,but it would,or should,get a fearful migraine. Yet that doesnt seem to happen. Gannets manage to bob to the surface with all their mental faculties intact,their brains entirely unhurt.
And how? Skull modifications,just as with the ram and the woodpecker. In this case,to mitigate the brain-shattering trauma of the collision with a wall of water,air sacs built into the gannets face act as cushion; its extremely long and narrow beak helps the bird enter the water with only a very stealthy kind of impact; and it has no nostrils that would allow water to gush inward and do serious damage to the delicate tissues inside.
Modifications of the skull are many,all produced by evolution to give each animal maximum advantage in adapting to its environment.
As always when at a loss,animal behaviourists like to say that the rams carry out their butting for reasons of power,territory or sexual ritual. Their skulls give nothing away,other than being stronger than usual. The rabbit skull,however,is awash with clues as to how this particular animal behaveswhich makes it a rather more intellectually satisfying skull to collect,if perhaps not as spectacular to have on display in Dudleys upstairs bedroom.
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No instalment of Paranormal Activity ever held a candle to the fear that accompanies the millennial social norm of ghosting. Whether you have ghosted, suffered the pain of being a ghostee or just heard the ghost stories, we are all unequivocally affected. Ghosting is the phenomenon of cutting off communication with someone, ranging from ignoring a text to disappearing entirely from someone's life. It is a newly developed social norm, given birth to by the rise of technology, which hides behind its false appearance as a platform which connects people. Yet, it is undeniably one of the most socially destructive and disconnecting social innovations imaginable. It is not surprising that, when we are so capable of hiding behind our screens, the social structures put in place when interaction was limited to real life experience have been replaced by new, updated versions which lack any form of physical interaction.
Technology has encouraged an environment which permits the treatment of other people as disposable. We no longer feel the need to talk to each other when it is easier to ignore a message. There is no plausible reason during a conversation to simply stop talking, get up and leave and yet the most self serving and unjustified excuses support the decision to leave a message unopened or on read. Whether you can't be bothered, you're avoiding confrontation or you don't want to say something difficult, the overwhelming urge to ghost is undeniable. This ease of ignorance means that we have a become a generation much less capable of navigating tough social situations. We don't have to learn to explain ourselves, to respond to other people's emotions and to reconcile confrontation. What's more, the ghostee is left with insecurity and unanswered questions. The message you are sending by ghosting someone is much worse than the message you never sent. You are showing this person that not only are you not interested, but that you're indifference is so great that they are not important enough for you to respond. When someone is simply cut off with no explanation, is it not so incredulous to assume they would be left feeling confused and insecure. By virtue of human nature in our society, we are far more critical of ourselves than others. The answers we would find, the flaws we could point to as the cause of rejection, are far more vast, personal and damaging than what the true reasons are. In many cases, the reason for ghosting may in fact be nothing to do with the other person, yet it is highly unlikely that this conclusion would be reached.
We owe it to ourselves and to each other to communicate our feelings. It is completely normal to lose interest romantically, or to feel annoyed at your friend, but these issues can only become more dangerous if left unattended. Explaining to someone why you no longer want to see them may help you to better understand yourself, and will allow them the opportunity to grow as well as protecting them from the spiral of negativity and self deprecation you put them at risk of. These social interactions teach us valuable lessons in empathy, communication and respect. It is a terrifying thought that we may be leading a culture in which our fear of rejection is so strong that we are inhibited. We shouldn't be scared to send a message because we inevitably assume they might not reply. We shouldn't be scared to express our emotions to other people, or live in fear angering and upsetting people. We are so casually slipping into a society that deems other people as insufficient for the basic level of respect of just replying to their message, thereby creating one which subjects us all to isolation and insecurity. The saying 'do unto others as you would have them do unto you' comes to mind and, although archaic, stands true today. Respect yourself, respect each other and reply to your messages.


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Products Baby Blankets
Item #: SLD01
Dimensions: 29" x 39"
Fleece Blanket for Dye Sublimation, Vapor® Large, Fleece, 29" x 39" White. These plush, sublimatable blankets can also be decorated as small throws. Perfect sublimation gift to expecting parents, bundle with a burp cloth, beanie and bib (SLD02, ... continue reading


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Hunting gloves – Buying guide, Reviews, and Comparison
If you want to find the best hunting gear, but you don't have time to go through the buying tips and reviews prepared by our research team, this short paragraph should tell you everything you need to know about the best hunting gloves on the market. After analyzing a lot of feedback coming from both amateur and professional hunters and target shooters, we have concluded that the Mechanix Wear Tactical MultiCam is the best because of its breathable Trek Dry material that follows the shape of the back of your hand to ensure you stay cool and comfortable in the field. The gloves feature a high-dexterity palm made of seamless 0.8mm synthetic leather that ensures a great combination of durability and flexibility for unhampered mobility. The dense TPR closure protects the covered hands from impact and abrasion. If the Mechanix Wear Tactical MultiCam is not available, you should consider the Carhartt Men's Magnet as it is a reliable alternative both in terms of performance and quality.
Comparison table
Our Top Choice
Equipped with a comfortable wrist closure, these best bow hunting gloves offer light protection along with a seamless fit around your wrists. The revolutionary Trek Dry material is breathable and follows the shape of the back of your hands to help you stay cool and comfortable during the hunt. The palm section guarantees a perfect combination of durability and flexibility for problem-free movement.
The gloves are not designed with adequate insulation for use during extremely chilly weather.
Designed for the effective disguise of the wearer in various settings, this pair of gloves is perfect for hunting and a variety of lighting conditions and elevations. Geared to trick the human eye, these gloves keep you hidden as needed.
2nd Best
Geared with a 100 percent polyester shell, these gloves also come with a polyurethane-strengthened palm, thumb, and fingertips along with a magnetic index finger and thumb for picking up metal items and getting a grip. The gauntlet cuff comes with a barrel lock closure for effortless tightening around the hand. The elastic wrist provides a snug and comfortable fit.
Nowhere in the description are the gloves said to be waterproof so don't expect them to be so.
Made with a 100 percent polyester shell, these best hunting gloves for cold weather have a polyurethane-strengthened thumb, fingertip, and palm plus a magnetic index finger and thumb for increased functionality. The barrel-lock closure provides a perfect and secure fit.
Also To Consider
Outfitted with a comfortable TPR wrist closure, this pair of gloves delivers light protection as well as an easy, comfortably seamless fit on the wrist. These good lightweight hunting gloves are constructed using the exclusive breathable TrekDry material that follows the back of your hand's shape so you remain cool and comfy in the field. Free movement is provided by the seamless 0.8mm synthetic leather that offers a blend of durability and flexibility.
According to one customer, the gloves have a weak Velcro closure that may not be for heavy-duty use.
With a versatile design that revolutionized the tactical hand protection industry, this pair of gloves comes with a versatile design that has proven to be functional for various users. The gloves provide matchless fit and versatility.
5 Best Hunting Gloves (Reviews) in 2020
1. Mechanix Wear MultiCam FastFit Gloves
Made with the revolutionary form-contouring Trek Dry material, the Mechanix Wear Tactical MFF-78-010 helps you make the most of every military or outdoor operation with its innovative MultiCam camouflage pattern. The material also keeps the hands comfortable and cool so you can make the most of every adventure.
To deliver a secure and comfortable fit, the gloves come with an elastic cuff that also enables effortless on and off without the need to make any adjustments. The two-piece palm is anatomically designed to prevent annoying bunching of material at any point, which can hamper free movement. To facilitate hassle-free glove storage, this pair ships with a convenient nylon web loop.
The lovely camouflage design can be easily protected and maintained thanks to the machine-washable feature.
The Multicam camouflage design enables the user to blend in with the surroundings for optimal use of the gloves. It disguises the user in a variety of environments, lighting conditions, elevations and seasons.
The gloves are machine-washable to enable easy maintenance and cleaning after use.
The comfortable TPR wrist closure provides an easy and secure fit on the wrists. The palm comes with an XRD open-cell foam padding to absorb vibration and shock and reduce hand fatigue.
These gloves are not adequately insulated to protect your hands from the chilly weather.Click to see the price on Amazon!
2. Carhartt Men's Magnet
Made with a 100 percent polyester shell, the Carhartt Men's Magnet A522 has high-quality features that enable optimal use in a variety of settings. These gloves are equipped with a polyurethane-reinforced thumb, fingertips, and palm for an extra-strong configuration. The index finger and thumb are magnetic to facilitate functionality.
The gauntlet cuff is outfitted with a barrel lock that enables easy fit on the wrist. The wrist is also made elastic to allow easy on and off of various hand sizes. The shell is made of 100 percent polyester for durability and reliable performance. The palm is made of 100 percent polyurethane for added strength. The lining is also made of 100 percent polyester.
These gloves protect your hands from the cold during outdoor adventures. They help keep your body temperature at a consistent level so you can focus on what needs to be done. You can put a liner under the magnetic fingers when the gloves are used for hunting purposes.
Their camouflage pattern supports your objective for stealth, so you can perform operations without worrying too much about being detected.
These gloves are versatile enough to be worn while jogging, fishing or doing yard work.
These gloves are not advertised in any way to be waterproof, but they do their job of hand protection adequately.Buy from Amazon.com for ($21.99)
3. Mechanix Wear Original Coyote Gloves
Providing superb hand protection, the Mechanix Wear Tactical MG-72-010 is ideal for military and tactical use as well as wilderness settings. The gloves come with a naturally-occurring shade of Coyote tan that disguises the hand of the wearer in an exceptional manner and various outdoor settings.
These gloves match your combat uniform well and are able to keep you concealed in an operating environment. You won't get easily detected so you can aim carefully and fire once you get a good shooting advantage.
These gloves are made of synthetic leather, which is durable and ensures a long life for the product. Superior in hand protection, the gloves feature Trek Dry technology, which guarantees breathability and comfort to the encased hands.
The Trek Dry material follows the shape of the back of your hand, so you always get a great fit along with exceptional dexterity or freedom of movement.
These Coyote tan military gloves boast a revolutionary TPR wrist closure that provides light protection during military and hunting operations.
The palm is made of seamless 0.8mm synthetic leather that guarantees a good combination of durability and dexterity. Its unique XRD open-cell foam construction absorbs impact through the palm to effectively minimize hand fatigue during use.
The TPR or Velcro wrist closure only provides light protection so it may be considered a weakness by some users.Buy from Amazon.com for ($17.98)
4. Glacier Glove Aleutian Full Fingered Neoprene Fleece Lined Glove
Constructed of neoprene, the Glacier Glove Aleutian GGAFU-P gloves are a high-quality product for the outdoor enthusiast. You will truly appreciate that they are built of G-Tek 2mm Fleece-lined Neoprene that guarantees a dependable waterproof design to make them ideal for outdoor use.
The seamless palm design of these waterproof hunting gloves provides sufficient support for various manual purposes. The full finger design guarantees a comfortable fit. Designed for both wet and cold conditions, these gloves provide dexterity and comfort where they are most required.
The gloves come with the exclusive Touchrite technology on the index finger and thumb, providing a tactile or intuitive performance especially when used for pulling the trigger of your hunting firearm or bow. Use these gloves for doing outdoor tasks such as hunting or gardening work.
These gloves provide superb functionality and performance thanks to their high-quality neoprene construction, which makes them suitable for applications that require waterproof hand protection.
The palm carries a seamless design that delivers enough support for a variety of manual operations.
The gloves come with a full-finger design that ensures a consistently comfortable fit. They also guarantee comfort and dexterity for optimal functionality.
The gloves do not have enough insulation against extremely cold temperatures, so one user recommends putting on a liner under them.Click to see the price on Amazon!
5. KevenAnna Tactical
The KevenAnna Tactical gloves carry a half-finger design that enables you to have greater dexterity and freedom of movement when you have to perform manual operations such as aiming and firing your hunting bow or hunting rifle. These gloves also work well for camping, motorcycle riding, and outdoor activities.
Furthermore, these gloves can be utilized as high-performance army gear when you need excellent military gloves, combat gloves, paintball gloves, batting gloves, assault gloves, and shooting gloves. They have a comfortably breathable design that is useful for preventing the easy onset of hand fatigue.
The gloves are made of high-quality elastic fabric that delivers optimum fit for all the five fingers. The double-layered knitted fabric ensures superbly cozy and dry hands in the half-fingered gloves.
The gloves come with an innovative half-finger design that provides maximum flexibility to the fingers while delivering heavy-duty protection.
The gloves have thick leather plates that shield the knuckles and palm from strong impact.
Adjustments on the finger and palm are easily carried out using the pull-ring design for those sections. This pull-ring configuration also facilitates easy putting on and taking off of the gloves from your hands.
One consumer commented that their gloves came with a characteristic odor that had to be washed off for removal.Buy from Amazon.com
Buying guide
When you want to hunt in frozen dark marshes, you ought to think about hand protection in addition to having the best bow or firearm. There are factors that all hunting gloves should come with, whether they are turkey hunting gloves or deer hunting gloves.
Look at products in the best hunting gloves on the market reviews that suit the type of hunting you intend to do
A variety of such gloves is available to suit various tasks, purposes, and conditions, along with different hunting methods.
Full gloves are designed to offer a high level of versatility and thus cover a wide variety of activities. Full hunting gloves are made of different types of materials. Most often, the pointer finger contains a different type of material such as neoprene, which enables greater maneuverability and tactile feel that wool gloves may not be able to deliver.
Many people love fingerless gloves because of how they are able to keep the hands warm without hampering dexterity. This enables the user to handle detailed, complex work that needs the use of fingers. Fingerless gloves are often constructed using simple materials and can have distinctive features including palm and knuckle guards.
Mittens are not exactly the ideal hunting gloves, but their versatility and warmth are advantages that some hunters may like over other glove types. The top rated wool hunting gloves carry a mitten design, but different manufacturers offer products with varying weave sizes.
Convertible mittens have characteristic rounded ends that can be buttoned back to give way to fingerless gloves. Convertible mittens keep the fingers warm while enabling the user to perform detailed finger work.
Riding gloves are made of a warm kind of material, which has to be thick around the fingers for protection from abrasion against the reins. They are also usually elasticized in the wrist area, so they do not slide off easily. Leather or wool is used in making riding gloves.
Typically constructed of thick, durable leather, falconry gloves come with a similar design and set of features although there will be color variations. They come with a guard spanning the forearm length, in addition to a metal run on the wrist that serves for tethering the bird to keep it secure.
Falconry gloves should fit well, so they do not slide around when the bird moves. They should be thick enough to provide protection to your hands from beaks and claws while being comfortable to wear for extended periods.
Whether you're buying cold weather duck hunting gloves or another type of glove, remember that the quality of material counts a lot too
Hunting gloves are made of a wide range of materials, each with its advantages and disadvantages.
Many types of gloves varying between hunting gloves to sporting gloves and standard cold-weather ones use wool in their construction. Wool is a natural material used in many different knit sizes, thicknesses, and hues. Wool is naturally water-resistant thanks to the lanolin compound in the material. It is great for keeping the hands warm.
Wool has a strong odor when it does get wet. It requires retreatment with lanolin since the compound naturally wears off with water exposure or regular wear.
Another natural material, leather is popular in hunting and sporting gloves and is also available in a range of designs and colors. It has a superb level of durability and strength and if properly cared for, can offer lasting use. Leather gloves stretch to fit with time. That said, they do not offer breathability and can be pricier than other glove types.
Gore-Tex is the brand name used to refer to a man-made material that has gotten quite popular as a dependable outdoor gear material. It offers extreme water resistance and doesn't need any wax or spray treatments to be consistently so.
Gore-Tex offers breathability to keep the hands comfortable and cool while reducing hand fatigue. It is also quite durable and extremely damage-resilient. However, Gore-Tex gloves are bulky and may not be comfortable against the skin without liners such as fleece.
Fleece is a material made from synthetic plastics and used in cold-weather clothing. Fleece gloves are fast-drying and lightweight, extremely soft on the skin, and the material is often used as a liner for less comfortable materials. However, water passes easily through fleece.
Fleece gloves can't be tumble-dried or ironed because they are weak against high temperatures.
When buying insulated hunting gloves, make sure they are of the perfect size for you
The size of the glove should be comfortable yet functional for you. An overly bulky pair can hamper the free mobility of your hands, or the gloves may just slide right off the hands. The majority of hunting gloves are listed in basic sizes; S, M, L, and XL. However, there may be listings that include measurements of the palm and fingers along with the simple glove size.
This will allow the consumer to calculate the size that suits their needs. If the buyer is quite close to the edge between sizes, it is good advice to go one size up instead of downsizing as this will provide more wiggle room as well as a comfortable fit.
A perfect pair of hunting gloves allows you to curl your fist fully. With time, leather gloves stretch to fit one's hands although the entire process may take quite a time, so some consumers just go a size larger at the onset.
Unavailable products
Reebow Tactical
Outfitted with a molded hard knuckle and reinforced palm, the Reebow Tactical gloves are designed for heavy-duty use. These gloves provide sufficient protection against impact and abrasion, so they are suitable for use as work gloves, motorcycle riding gloves, and tactical gloves.
You will surely appreciate the remarkable flexibility and dexterity of the gloves even when used for motorcycle or ATV riding or working in the yard. The gloves are elegantly stitched for a great look. They are lightweight yet amazingly durable. They have a comfortable and breathable design to reduce hand fatigue.
When working or doing outdoor activities, you will appreciate how the gloves deliver a snug fit and enough support.
These hard-knuckled tactical gloves are constructed of durable microfiber leather material that makes them suitable for dirt bike, ATV, and motorcycle riding.
The gloves are made resistant to wear. They are slip-proof for full support during use. They are also lightweight and are fast-drying for dependable outdoor performance.
These gloves provide secure protection when used in heavy-duty applications. They allow you to do intensive outdoor tasks
The gloves tend to come larger than expected, according to some users. However, they offer dependable functionality and a great design for outdoor use.


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Salsa Ladies Styling Interview with Melissa Rosado
No comments yetPosted in Ladies Salsa Styling
Melissa Rosado who is a top international Salsa performer and teacher, talks about how not to affect the lead when we incorporate our ladies styling into our partnerwork. This is a great tip for ladies!
Highly recommended – Melissa Rosado's Salsa Styling Online DVD!


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Woodbury Guild
The Woodbury Guild is a great mix of volunteers: stay at home moms, young professionals, and working parents. Many of its members have children and love knowing they have access to the amazing services at Children's Minnesota. The guild hosts an annual spring event at Lift Bridge Brewing Company in Stillwater. They also hold an annual golf tournament called Swing for the Children at Oak Marsh Golf Course, the fourth Monday in September.
The volunteers in the Woodbury Guild socialize together and enjoy each other's company outside of planning events for CHA. When they are meeting to organize our events they usually meet at Total Wine and More in Woodbury or Oak Marsh Golf Course in the evenings on the first Tuesday of the month. This guild welcomes members from all over the East Twin Cities!


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"I guess that's really it; I feel like I don't have to prove myself. I don't have to prove anything," Dion says. "When you don't have to prove anything, it gives you a lot of space."
Search results for:
Hit-maker for the ages and Strip resident star Lionel Richie and will be the headliner at the Keep Memory Alive Power of Love gala March 16 at MGM Grand Garden Arena.
Split/Face" will be displayed at the new Criss Angel Theater at Planet Hollywood on Dec. 19, the first night of previews for the new"Mindfreak Live."
Boy George has been teasing about a residency for the past couple of years, especially referring to a Vegas residency in occasional Twitter posts. But this weekend Culture Club is co-headlining a one-night, rock-and-dance party with the B-52s at Downtown Las Vegas Events Center.
"Don't Lie to Me," released Thursday, finds a passionate Barbra Streisand questioning the nation's leader and pleading for change.
A rapper and producer who collaborated with some of the most famed acts in West Coast rap history has been arrested on drug charges outside Atlanta.
I refer to Dave Loeb's performances on "Family Guy" as, "The Bait." It's what we put on the hook to to remind Las Vegas music fans of Loeb's true passion — his UNLV Jazz Studies program at UNLV.
We anticipate Jennifer Lopez will be back on the Strip either next year or 2020, at The Park Theater. An educated deduction, that.
Majestic Repertory Theatre, 1217 S. Main St., premieres "Measure for Measure," a new piece of disco-tinged immersive theater based on the Shakespeare play of the same name, on Thursday. Because the show is customized for each attendee, tickets are limited to 18 for each showing.
Round-up of music happening this week.


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This 500-piece puzzle deserves kudos–and that's no hyperbole! For ages 14 yrs-adult, from Eeboo.[Product Details...]
This spiral-bound sketchpad offers 60 high-quality, heavy weight pages! For ages 3 yrs-teen, from Eeboo.[Product Details...]
Make a mini feast with air-dry clay and awesome accessories! For ages 8 yrs-teen, from Fashion Angels.[Product Details...]
This giant bounce ball creates instant high-energy fun! For ages 3 yrs-teen, from B4 Adventure.[Product Details...]
Gravity will be so confused! Engineering, physics, and fun brought to life! For ages 8 yrs-teen, from Thames & Kosmos.[Product Details...]
Racers grab the handle and go–to the finish line! For ages 5-12 yrs, from B4 Adventure.[Product Details...]
Stack big, chunky puzzle pieces in ABC order! For ages 2-10 yrs, from BeginAgain.[Product Details...]
Hundreds of ways to create, all neatly organized in a stylish and sturdy case! For ages 8 yrs-teen, from Kid Made Modern.[Product Details...]
Be the first to clear your planet of free-floating debris! For ages 6 yrs-teen, from USAopoly.[Product Details...]
A robot that turns into a racecar with the push of a button! For ages 8 yrs-teen, from Odyssey Toys.[Product Details...]
Dexterity develops as little hands push the characters down, and they pop back up! For ages 1-3 yrs, from Djeco.[Product Details...]
Discover germs, create cultures, and grow gross stuff! For ages 6-12 yrs, from Heebie Jeebies .[Product Details...]
Set up interlocking bases, insert the buckets, and toss color-coded balls! For ages 8 yrs-adult, from Franklin Sports.[Product Details...]
Golf balls are now birdies that fly through the air! For ages 6-12 yrs, from Hog Wild Toys.[Product Details...]
Write a word that fills in the blank. Who matches? For ages 8 yrs-adult, from USAopoly.[Product Details...]
Quick! Is it a furry friend or a sesame snack? For ages 7 yrs-teen, from Blue Orange Games.[Product Details...]
Having your own drone is awesome! For ages 8 yrs-teen, from Island Genius.[Product Details...]
Rest your device on this little speaker for instant big stereo sound! For ages 5 yrs-adult, from On Trend Goods.[Product Details...]
Become a sweet scientist! Brew your own delicious root beer! For ages 8 yrs-teen, from Copernicus Toys.[Product Details...]
In warm water, the colors change right before your eyes! For ages 3-7 yrs, from Reeves International.[Product Details...]
In warm water, the colors change right before your eyes! For ages 2-7 yrs, from Reeves International.[Product Details...]
Iconic arcade action in a tabletop game! For ages 6-12 yrs, from Ravensburger.[Product Details...]
Modern engines communicate with lights, sounds and motion! Additional trains and tunnels for extra fun. For ages 3-7 yrs, from Ravensburger.[Product Details...]
This modern engine communicates with lights, sounds and motion! For ages 3-7 yrs, from Ravensburger.[Product Details...]
Get ready to save the day with this realistic replica! For ages 4-7 yrs, from Bruder Toys.[Product Details...]
Special delivery! A fully functioning replica that brings work to life. For ages 4-7 yrs, from Bruder Toys.[Product Details...]
These adorable critters are well-loved! Attention to detail in every miniature world. For ages 3-10 yrs, from EPOCH Everlasting Play.[Product Details...]
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Setting up a new Windows 7 box? Ninite.com is amazing!
March 1, 2012
This past weekend I decided that I needed to make my MacBook Air more functional, so I Bootcamp'd it to Windows 7, my preferred working environment. Of course after installing Win7 I needed to make it useful by installing a plethora of (my) standard tools. Normally, I do this by hand (I have a list, and don't really mind it), but I figured I'd give Ninite.com a shot since someone had suggested it to me. Oh, My, dear and fluffy lord. Ninite.com is amazing.


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I have a bramble in my garden which is starting to take over. Please can you tell me how to stop it without killing the rest of my shrubs?
Certified GKH Gardening Expert
You can use Round-up, but only in a very localized fashion. Cut the bramble back as best you can and then paint the fresh cuts with Round-up. The bramble will suck the Round-up into its roots and this will go a long way to killing the roots. You may have to repeat 2-3 times, but every time you do it, it will weaken the bramble.
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Hi lovelies. How are you all? I have had the longest week ever, it has gone so slow! So I am glad it's time for a rest. Also this is my 100th post, I don't believe I have posted so many already.
It's the weekend! So that means it's time for Copycat Saturday. This week I was inspired by this pin, as often is the case with pinterest it doesn't link back to the original creator.. but judging by the watermark I think they originated from this blog, however I cannot find the original post.
For more pictures and full details on this manicure click below.
This is a pretty simple design for me, but I thought it was really pretty.
I didn't have a colour like the original manicure so I decided to go for Zoya - Cassi.
On my ring finger I painted the shape of my half moon first and then filled in the rest of my nail.
I secured my rhinestones with topcoat. I was having an issue with my topcoat going wrinkly on my ring finger, I have no idea why!
So despite these being really simple, I still enjoyed wearing them. Plus they took about a fifth of the time of my normal manicures, which is always a bonus.
What do you think? Let me know in the comments.
Thanks for looking.