text:
I graduated from U.C. Riverside with a Bachelor of Arts degree in sociology and psychology. In my junior year of college, I spent a year abroad at the University of Göttingen in Germany. After that I went to Cal. State San Bernardino for my multiple subject teaching credential.
My hobbies are gardening, hiking, traveling, and painting with watercolors. However, my favorite activity is reading and learning new things. I hope to share my love of learning with all my students.
Riverside Unified School District prohibits discrimination, harassment, intimidation, or bullying in all district programs, activities, and employment on the basis of actual or perceived ancestry, age, color, disability, gender, gender identity, gender expression, nationality, race or ethnicity, religion, age, sex, sexual orientation, parental or marital status, pregnancy, or association with a person or a group with one or more of these actual or perceived characteristics.
If you have any complaints or questions regarding this policy you may contact
Senior Administrator for Pupil Services or the District Complaint Officer at
(951) 788-7135 or (951) 352-1200.
Remember If you see something suspicious, say something.
Contact WeTip at: 1-800-78-CRIME (1-800-782-7463) or log-on and submit a tip at www.wetip.com
For safety policy click here
Need help with drug abuse or mental health? Please visit RUSD's Care Solace page for support.


text:
Christmas is over now, and while you still might be enjoying your time now with your friends and family throughout New Year's and the remainder of the Christmas season, it will soon be time to take down your Christmas decorations and store them away for next year. We know that putting all those decorations can be quite a chore, but if you do it properly, you'll have an easier time assembling things next year!
Check out our 7 tips and tricks below for properly packing and storing your holiday decorations!
Be Smart About Tree Storage – Artificial trees can last for years and years to come if they are taken care of properly. Before you store the tree, think about its weight. Many are too heavy to wrangle into a trap-door attic or lug down a flight or two of stairs. To save yourself a headache (and a backache!), consider storing your Christmas tree on the same floor that it is used such as in a storage room, a closet, or even an attached garage.
Store Christmas Lights Tangle-Free – Storing lights after the holidays can be such a pain. It's even more of a hassle when you revisit them in the following year and try to untangle the cords without breaking off or damaging the lights or wires. Avoid the tangled mess by using a piece of masking tape and permanent marker to label each strand. You can indicate how long it is or where you usually use the lights. Afterward, wrap the strands securely.
Protect Your Ornaments – Ornaments come in many different shapes and sizes and can be very fragile. Instead of tossing them in a box, try storing them in a container that is designed with layers and fill the empty space around each ornament with tissue paper to keep them secure. When looking for your container, try to choose something that is lightweight, has fabric-lined trays, and has plenty of padding.
Wrap Up the Gift Paper – If you're not able to store your giftwrap, paper, tags, bows, ribbons, and tape in one small permanent area, you can put it all into an under-the-bed storage system. If there is no room under your bed for all of your supplies, your gift wrapping supplies can be stored in an organizer in a guest room closet.
Make Setting Up Villages Easy – Do you have Christmas villages? If so, you might know how much work it takes to set up the villages and all of the snow, landscaping, lighting, and accessories each year. Make set up easier next year by storing your villages inside of their packages and placing them inside a storage bin FIRST. Then place any snow, ground, and other accessories you might have on top of the village boxes. By doing this, you can set up the things that naturally go out first without having to dig through all of the village pieces.
Keep Your Wreath Safe – While relatively sturdy pieces, wreaths can be quite fragile, especially if they feature dried berries and twigs. Keep your wreaths safe, decorated, and fluffy for next year by storing it in a crush-proof container! Even though it might take up more space, the container will do a great job protecting what's inside and can be stacked.
Keep Linens and Cloths Fresh – Want to keep your tree skirts, seasonal tablecloths, and other festive items fresh, fluffy, and protected from moisture, moths, and mold? Consider storing them in a hanging vacuum-storage bag. You can even store your plush winter throws in these storage bags, too. Next year, you can unseal these seasonal items, and they will be as fresh as they were when you stored them!
Your home is so much more than just a place to store decorations. Make sure that it is properly protected with the right home insurance policy. Call the experts at Madison Mutual Insurance Company by dialing 1-800-766-MMIC or by visiting us online at www.madisonmutual.com!

subset: commoncrawl


text:
- Our Union
- Campaigns & Actions
- Janus v AFSCME What's at Stake?
- Photos
- calendar
- Become A CAT
- Facts at your Fingertips
- Talking Papers
- Solidarity
- Past campaigns and actions
- Newsstand
- Resources
- Contract & Bargaining
ARTICLE 11 - Child Care
The Employer shall continue to provide tax sheltered dependent care accounts for employees' payroll deduction use, if allowable under state and federal statute. Each campus shall provide to employees information that is available on child care referral services existing for both well and sick child care. This information will be provided at employee orientation and upon employee request by the appropriate Human Resources Department. Additional child care information is included in Appendix E.
Whenever the University establishes an active Child Care Committee on a campus, the Union shall be offered representation on that Committee.


text:
There has been a concern for some time throughout America regarding the quality of public education. Students are graduating from highschool without adequate knowledge of the three R's. Universities are recruiting a multitude of incompetent pseudo-scholars. Employers don't understand why the new generation of workers do not possess the basic skills to perform the job. It would appear that American students are not learning as much as their parents did. And yet, teachers are still teaching, taxes are still being paid, and more funds than ever are being appropriated for public education. What's going on in America's classrooms?
Up until the 1980's most schools used a standards based curriculum. In the traditional classroom setting, educators focused on the input side of education, teaching a specific body of knowledge. Students were graded against predefined standards and passed or failed based on their ability to meet those standards. This method of teaching produced a graduate with a well rounded education, and prepared him for further development of career skills.
With the exception of those who did not apply themselves, the system worked.
Today America's educators take a new approach in the classroom. The focus of education has moved to measuring what students can do, rather than what they understand. This is the core principle of Outcomes Based Education (OBE). An outcome, by definition, is something that follows as a result or consequence. So OBE then, is an approach to education where the end result is the most important factor. This is very important in understanding what OBE is, and what it intends to do. In the OBE classroom,
every aspect of the curriculum is geared toward achieving a small group of specific goals. To gain an insight into OBE, it is necessary to learn something about its origins and those promoting it.


text:
- The $40 Shipping Covers Mel, Syd, Bris, Adel, ACT. and some surrounding regional areas. Please call/email us for a competitive freight quote outside these areas like WA, TAS, NT and remote areas
- Additional features:
- Premium Timber, Treated for long term protection against rot, fungal decay and insect attack
- Our durable timber products have been UV-stabilised and weather-treated to withstand the harsh Aussie climate
- Pre-painted window frame, Matte Stained wood for a smooth, natural finish
- Meets EN71 European Safety Standards
- Assembled Size: House – 110cm x 107cm x 141cm (L x W x H) Complete Footprint – 197cm x 132cm x 141cm (L x W x H)
- Recommended Age: 12 Months and Above
We have a fantastic range of Fitness Equipment at unbeatable prices. Please give us a call or email if you have any questions about our Fitness Equipment. Ph: (03) 9887 5058. WE WON'T BE BEATEN ON PRICE!


text:
Calendar Month View
Card Making Class
Adults 55+
- Date: 04/09/2019 9:00 AM - 12:00 PM
- Location: Senior Center
240 S. Route 59
Bartlett, Illinois 60103
Return to full list >>
Card Making Class
Tuesdays: April 9 & 23 (Series)
9:00 A.M. – 12:00 P.M. Donation: $23
Join Devona Murrell in creating themed cards. The themes this series will be birthday cards and an Easter card. You will have a chance to make six cards per class. Supply cost is included in donation fee. Devona will guide you through designing your cards and creating your unique pieces of art. Registration required.


text:
Out of stock
Additional Information
|1000 g
|21 x 25 cm
There are no reviews yet.
Be the first to review "Igor Mukhin. Born in the USSR (The First Edition (2005)! Rarity!)" Отмена ответа
Need Help? Contact Us Leave Feedback
Categories: Artist and self-publish books, Documentary.
Contact Us
Leave Feedback
The first edition of the famous book by the wellknown Russian photographer Igor Mukhin.
Mukhin first made his name in the late 1980s. As part of Immediate Photography, an underground grouping that included Boris Mikhailov and Alexander Slyusarev, he documented the crumbling remains of Soviet monumental propaganda. Soon, however, he went on to find his own individual style. Rejecting the social criticism of dissident art, he began to strike a more optimistic note, focusing his lens on the day-to-day lives of Russian youth.
This theme is on ample display in "Born in the U.S.S.R." The teens and twentysomethings, who populate Mukhin's photographs are a lively bunch — they swim, sunbathe, arm-wrestle, hug their boyfriends awkwardly in the street, wave banners at various kinds of political demonstrations, gaze at the fireworks on New Year's Eve and stick their tongues out at the camera.
192 pages, 22х25 cm
Language: English and Russian
Related Products
- 55,00 €
- 30,00 €
- 45,00 €
- 40,00 €
Insight. Black and White Ukranian Photographers. XXI centuryArt photography, Artist and self-publish books, Documentary
- 30,00 €
- 30,00 €
- 25,00 €
- 25,00 €
- 25,00 €
The End of a Century: Metamorphosis of Being. Leningrad – Saint PetersburgDocumentary, Historic photography, Petersburg Photography
- 15,00 €


text:
Disclaimer: I do have a small credit in this book. A long time ago, the author mentioned that he was looking for people to help out, so I stepped up. It was only participating in discussions like "What do you think about version control's use in industry?"; I didn't help with any actual writing, and yesterday was my first time reading virtually all the final content. No monetary interest, of course.
I've been doing a lot of reading over the last few weeks, some of it technical, some of it non. I've been meaning to type up a few book reviews, and yesterday I finished reading my copy of Josh Carter's "New Programmer's Survival Manual," published by PragProg. Since that's a bit lighter material than Spinoza, I figured I'd write it up first.
The Survival Manual is intended to be the book you should buy after you graduate from college, but before you get a job. It's a bunch of tips, arranged by topic, that give you a taste of what 'real world' programming is like, as well as what working life is about. I think that it accomplishes this goal wonderfully, but if you'd like to hear specifics, read on.
One of my roommates is not particularly technical. She picked the book up, and read the introduction and the first tip. She then said something that, after reading all of it, I agree with 100%. "I like this guy's writing style. It's very... readable." Josh's prose feels... comfortable. Like you're talking to a down-to-earth friend, or co-worker. Which is great, given the topic. I really enjoyed the actual reading of this book. Especially in technical books, this is a great quality to have, but I guess this book isn't exactly technical; it's much more about soft skills than hard.
I also really enjoyed the cookbook-style organization. Basically, the book is one big ball of tips that you can read individually. They're grouped together with three or four others that are on the same topic, but each stands alone, and they cross-reference each other when appropriate. While I sat down and read it front to back, I'd imagine it's pretty easy to cherry-pick certain things and read it in a more non-linear fashion.
I also feel that 'pragmatic' is a good description of most of the content. In particular, as much as we all love DVCS, the book frankly states that most places still use centralized version control, which is totally true. He makes sure to carefully not be particularly dogmatic about the specifics of anything, focusing on strategy more than tactics. For example, "Get good with your editor, make sure it can work effectively without using the mouse, for speed," not "zomg emacs r teh suck, use vim."
Finally, Josh links to copious other works, and provides references for all of it. Some stuff is just general internet culture, but the bibliography alone would assist any programmer in finding some useful primary materials to read. Here's the first six: SICP, Getting Things Done, Extreme Programming Explained, TDD By Example, The Mythical Man-Month, and Foundations of Object Oriented Languages: Types and Semantics. Oh, and just because there's some XP/Agile stuff in those particular books, don't think it's slanted towards that: waterfall is explained as well. Just so happens those books were first.
There's not that much bad that I can say about this book, really. There are a few small things, though:
There's a 'white belt brown belt black belt' metaphor that's supposed to indicate a difficulty of each tip. Given that there's only two black belt tips, I didn't feel that three levels were really needed. I also just thought that there wasn't a huge difference between white and brown, either, so I'm not sure that it really adds any utility.
Because this book is fairly high-level, it might be hard to actually apply
these lessons. This might be more of a problem with readers than with the actual
book, but I can't count how many times I (and others) have said "Of course,
write descriptive variable names!!1" and then typed
x = 5. It's just the way
things are. And since this book is essentially a collection of advice at that
high level, I can see someone reading this book, and then just simply not
following the advice. I'm not sure if this is something Josh could have fixed,
or if it's inherent in this kind of book. Also, it won't affect a diligent
reader. It is something to think about, though.
This book is totally worth it. The style reminds me of the seminal Pragmatic Programmers. If you're a freshly minted graduate, put this book at the top of your reading list, then also grab a copy of all the books in the bibliography to read later.


text:
Full text loading...
n Management Today - Cyril Ramaphosa gets Leadership in Practice award from Unisa
"Business leaders, as much as any other leaders in society, need to understand the people. They cannot afford to stand aloof. Leaders in business, as much as other leaders, need to appreciate the collective knowledge of the people and understand the potential strength of their collective will. We know that the world is becoming increasingly interconnected. It is a risky endeavour to try to navigate that world without the support and guidance of others."
© Publisher
Article metrics loading...


text:
Religions and its beliefs are represented on the Internet in many ways, one of them is to create nice and clean church website. With the rapid growth of WordPress as the most preferred CMS to build a website, WordPress Church Themes have become a trending topic these days.
Are you planning to launch your church website and looking for a decent WordPress Church Theme ? Do you want to revamp your church website on wordpress and want a perfect wordpress church themes to match your website aesthetic. Are you looking for a ready to use wordpress church theme for your newly developed wordpress website ?
If you find yourself in any of the situation mentioned above then you must continue reading this very article further, as we would be talking about the best wordpress church themes available in the market today. In this blog post we will showcase the world's top Church themes for WordPress and also discuss the benefits of using WP church themes.
Why use WordPress Church Theme ?
- These themes are highly customizable, which gives you the ability to tweak its function or appearance as per your need.
- WP Church themes are designed for everyone and not just technical people. So one of your Family member can also manage your website easily.
- WordPress church themes are low cost and is usually maintenance free.
- Church themes made for wordpress are powerful right out of the box and fives your super control over your website.
- Such themes are extremely flexible and search engine friendly.
- Professional wordpress church themes never compromise on security , so you can be rest assured that your your website and data is safe and secure.
- Easily host multimedia, video messages, webinars with just a few clicks from right within the dashboard.
- With a powerful commenting system, it helps your church website build a community rapidly.
- The best wordpress Church theme has easy integrations with third party payment processors for easy donation management.
- WP Church themes Save you time, money and effort with the help of ready-to -use wordpress church theme.
# Bethlehem is an easy-to-use, responsive and highly flexible wordpress theme built for Churches and Prayer Groups as well as Non-profits.Bethlehem WP theme has a powerful dashboard panel that allows you to customize your website to match your brand with just a few clicks.
# ChurchSuite is a super premium, fully responsive as well as Retina-Ready WP Church theme with a minimal, clean look that lets visitors focus on content without much distraction. ChurchSuite has been developed with several awesome features without compromising with website load speed and performance.
# Risen Church theme specially built for WordPress based website which has all the essential features a church website needs. With mobile friendly responsive design, event registration management, sermon archive, recurring events, donations and media gallery , Risen WordPress Church Theme is one of the first choice for many church website owners.
We would love to know which Church theme you guys finally decide to use for your church website.


text:
In a speech at a rally for the National Association of Home Builders last Thursday, posing in front of an unbuilt home that no one will ever buy because no one can get a mortgage, alleged fiscal conservative Newt Gingrich argued on behalf of home ownership and government support of it, and in the process ridiculed people who ride the subway, of which there are apparently some in this country.
Gingrich determined that people who ride the subway also don't own homes, and lazily take public transportation that, well, their tax dollars help pay for from their "high-rise apartments" to their jobs at "fancy newspapers in the middle of town." These elitist assholes don't see the value of owning their own home (or owning their own job?), Gingrich surmised. Maybe they should just…like…move to Paris! Where there is more subway than there is Subway, and where being a snob doesn't get you verbally excommunicated by a windbag with too much time on his hands. READ MORE »


text:
At least 43 people, including newborn babies, have died after a bus plunged 200 metres (660ft) into a ravine in northwestern Guatemala.
Rescue workers said the overcrowded bus veered off a road in in San Martin Jilotepeque, roughly 37 miles (60km) northwest from the capital Guatemala City.
At least 40 more people are believed to have been injured in the crash, which completely destroyed the bus. It had been traveling to San Martin Jilotepeque from Chimaltenango.
The cause of the accident is not yet known. Rescue workers said conditions were sunny and dry at the time of the crash. A fire department spokesman said 90 people were aboard the bus, which only had a capacity of 54.
Forty people were reportedly confirmed dead at the scene, with a further three more at the hospital. At least three of the dead included babies, the fire department spokesperson added.
President Otto Perez declared three days of official mourning in the wake of the tragedy.
The mayor of San Martin Jilotepeque, Otto Vielman, said: "From what we see, the bus crashed against a wall of rocks first and then fell over the cliff."
A similar bus crash on the same road killed 40 people in 2000, Vielman added.
Guatemala is the fifth-worst country in Latin America in terms of road accidents, with 20 in 100,000 inhabitants killed in crashes in the Central American nation.


text:
1321 S Darling Dr NW, Alexandria, MN 56308, United States
Visit their website.
Shady Lawn Resort offers a healthful, relaxing vacation at economical rates. Shady Lawn is a clean, pleasant, family-oriented resort with nicely shaded grounds overlooking Lake Darling. Excellent Bass, Crappie, Northern, Sunfish and Walleye fishing is found in Lake Darling, as well as the adjoining lakes (over 5,000 acres), which are readily accessible by boat.
There is an excellent sandy swimming area with shallow beach and no drop-offs (ideal for children). An anchored raft is available for sunbathing and water games. A large deck overlooking beautiful Lake Darling is also available for sunbathing and relaxation.
Pets on a leash are welcome!
Open May- September


text:
Cross Country Base Expert/Pro Next Level Program
Drew EdsallAll plans by this Coach
6 Weeks
Plan Specs
cycling mountain biking
Refund Policy
This plan is protected by our Refund Policy and may, with the author's approval, be exchanged for a plan of equal value from the same author.Still have questions about this plan?
Plan Description
This training program is the best way to start your training for the year.
This 6 week mountain bike training plan is for the Expert or Pro level athlete that is very experienced rider with lots of hours on the bike in the past. This is a very tough training program with the last week reaching 20 hours total volume. Main focus is on volume combined with muscular endurance and tempo work. If you need to be pushed to the next level and feel you are ready for the hours in this program, this program will be great for you.
You will establish HR training zones mid week in the first week, and then the remaining workouts will be focused on building your Base Miles.
The workouts are 6 days per week with Monday as your off day. One of the main goals of this plan is to build your foundation, so there will be a long ride every Sunday focusing on building your aerobic capacity. The rides during the week can be performed indoors or outdoors, your choice.
The rides in the training plan will build your aerobic capacity with long weekend rides, muscular endurance with tempo efforts and big gear efforts, and developing the means to push harder in your next training block.
You will end this plan with the ability to ride longer, harder, and most importantly the training plan setup to move forward with a higher intensity training plan after this.
This training plan will set you up to continue to build your fitness higher and higher following it.
Average Weekly Breakdown
|Weekly Average
|Longest Workout
|16:53 hrs
|6:00 hrs
|Workouts Per Week
|Weekly Average
|Longest Workout
|16:53 hrs
|6:00 hrs
Sample Day 1
Ride Zones 1-2 today. Get 50% or more of the time in Zone 2.
Sample Day 2
BT: Warm up well. Then ride a 30 minute time trial on flat course. Punch HR monitor 'lap' button 10 minutes into Time Trial. Average heart rate for last 20 minutes predicts Lactate threshold heart rate (LTHR).
After you are done with the test, update your Training Zones by doing the following:
-Take your avg HR for the last 20 minutes
-Go to My Settings in Training Peaks
-Go to Zones
-In the Threshold Box put your avg HR for the 20 minutes
-The select Joe Friel Cycling Zones(7)
That should set up your zones for you.
Sample Day 3
Ride Zones 1-2 today. Get 50% or more of the time in Zone 2.
Sample Day 4
Ride mainly in Zones 1-2.
Within workout include 10-15 x 10-20 revolutions of the pedals at a very slow cadence of 50-60 rpm at RPE of 6-7. HR will not respond in time for these efforts, so use RPE (see attachment at beginning of week for details on this).
Take 2-3 minutes rest between each effort.
Sample Day 5
Ride Zones 1-2 only today. Within the ride include 20 minutes of one legged work, where one leg does 90% of the work while the other leg "loafs". While performing this drill, attempt to push your toes to the front of your shoe at the top of the pedal stroke, and scrape dirt off your shoes at the bottom of the pedal stroke. Attempt to eliminate all dead spots and become more efficient!
Sample Day 6
Ride Zones 1-2 today. Get 50% or more of the time in Zone 2.
Sample Day 8
Ride Zone 2-3 today. Use big gears only keeping your cadence between 70 and 80 for most of the ride. For any climbs you encounter, push even a bigger gear at 60-70 rpm.


text:
Nassau County is located in the north eastern part of Florida between Baker and Duval Counties. Created in 1824 the county is named after the Duchy of Nassau, Germany. Nassau County includes 651.55 square miles of land and 74.30 square miles of water. The population of Nassau County was 73,314 according to the 2010 U.S. Census.
The county seat of Nassau County is the city of Fernandina Beach, FL. Fernandina Beach has a population of about 12,000 people. Fernandina Beach is the only United States city to have 8 different national flags flown over the city. From 1562 the following nations have laid claims to this area, these nations are; France, Spain, Great Britain, Patriots of Amelia Island, Green Cross of Florida, Mexico, Confederate States of America and the United States of America. George Rainsford Fairbanks, a Confederate Major, historian and lawyer was from Fernandina Beach.
Nassau County is part of the Greater Jacksonville Metropolitan Statistical Area. Nassau County is home to Amelia Island, where the 1988 famous child movie star Pippi Longstocking had her adventures at. Nassau County has several historical places to visit, some of these are; Amelia Island Lighthouse, Bailey House, Fairbanks House, Fort Clinch, Hippard House, Merrick-Simmoons House, Mount Olive Missionary Baptist Church, Original Town of Fernandina Historic Site, John Denham Palmer House and Tabby House.
Nassau County is bordered by Camden County, GA to the North, the Atlantic Ocean to the East, Charlton County, GA to the West, and Duval County, FL to the South.
Municipalities of Nassau County, FL
Incorporated Cities and Towns of Nassau County, FL
Callahan, Fernandina Beach and Hilliard
Unincorporated Towns and Vilages of Nassau County, FL
Amelia City, American Beach, Andrews, Becker, Boulogne, Bryceville, Chester, Crandall, Crawford, Dahoma, Dyal, Evergreen, Franklintown, Glenwood, Gross, Hedges, Hero, Ingle, Italia, Keene, Kent, Kings Ferry, Lessie, Mattox, Nassau Village-Ratliff, Nassauville, O'Neil, Verdie, Yulee Heights and Yulee
External Links for Nassau County FL
Official County Site - http://www.nassaucountyfl.com


text:
With the appearance of social media, the number appears to be dropping, and it's possible you'll be stunned at how few steps exist between you and someone else – particularly effectively-known people. If firm coverage forbids such an action, then ethically, the enterprise needs assessment; financially, they will easily fill the slot with another pharmacists with out a conscious problem and not have to terminate the employee. I extremely advocate Powerteam International and their events to anyone who's severe about taking their business to the following stage and creating even more success in their lives.
We'll additionally include input from news of us who efficiently moved into new areas, the challenges they confronted and the way they overcame them. When I am seriously considering making an attempt out a new enterprise (and spending my hard earned cash), I sometimes take a look at multiple reviews on a number of sites. However, I also discover that Fast Company would not deliver on the identical level of journalism as the other enterprise magazines, and has proved itself to be of little or no use. As a big fan of Li Ka Shing, I even have read many books about him and his philiposophy in enterprise is profound and yet easy.
The Boston University on-line business faculty can also be accredited by the impressive Association to Advance Collegiate Schools of Business (AACSB) International and the New England Association of Schools and Colleges (NEASC). The time or date displayed (together with in the Timeline of Articles feature) reflects when an article was added to or updated in Google News.
When I say that a frontrunner cannot be putting out fires, I imply that, in an enormous company, she or he cannot be centered on quarterly earnings. The work of leadership is strictly to create and implement the 5 plans that boil down the essence of the success of a particular enterprise in order that employees can contribute to that success and clients can benefit from it. And it is easier to say that than to do it!
There is an enormous distinction in the bundle they provide depending in your country of origin for the bulk of Saudi Companies. If you've gotten the required dedication, expertise and schooling, you may also climb up the company ladder to top administration positions. The world is smaller than ever, now, and it has an actual affect on how folks store.


text:
Installing a Kee Gate self closing safety gate can help you to comply with Health and Safety regulations by protecting voids on access points in warehouses, on mezzanine floors, access ladders and roof hatches. By closing automatically, the self-closing safety gate overcomes the potential for human error which is a problem with traditional methods such as chains, bars and sliding tube.
Previously, chains, bars and sliding link tubes provided safe access to open voids. These methods are reliant upon users remembering to replace the chain or bar once they've passed through. Being spring loaded, our self-closing safety gates automatically close behind the user which is why they are now the preferred solution.
Installation of the Kee Gate self-closing safety gate is quick and simple, the gate is supplied 1m wide and can be easily be cut down to the required size on site. To trim the gate all you need to do is undo the two grub screws using an Allen key and then cut the gate to the desired size. Once done, simply re-attach the gate and tighten the grub screws.
Watch our Kee Gate Installation Video to see for yourself how easy it is to install it.


text:
This is the link for this week's Teapot Party challenge over at Splitcoaststampers. Looking for a place to send your cards? Join this weekly challenge - the mods select some one in need of cheer, or who deserves a special recognition. This card will go to Carolyn who has had many health problems and could use a little cheer. Butterflies were included in the suggested themes.
1-2-3 challenge: Image by Stampemdous; I used shimmery silver cs to emboss the butterflies (Darice) - my favorite cs for embossing. The second cs is poppy red. There are 3 shiny gems in a color close to the strawberry milkshake of the color challenge. The sentiment is from Inspired by Stamping.
Sketch/color: Share Joy Challenge
1,2,3: Come and get it
Embossing: Frilly and Funkie
Any: Inspiration Destination
Simply Papercrafts for all Occasions
One Crazy Stamper
The Sisters of Craft


text:
Calistoga Cafe
This weekend I got to try a new restaurant, not once, but twice!
The first time we visited Calistoga Cafe was for lunch on Saturday after a 5K we did for our local Humane Society 🙂
Cute doggie alert!
Calistoga reminds me a lot of Panera but with a different flair. They have mostly sandwiches and soups and serve breakfast too, including their own homemade bagels and pastries. They have a coffee bar too and offer a few flavors of smoothies. Everything is fairly reasonable in price too, especially for the volume of food you get.
Posted on May 12, 2009, in Breakfast, Lunch, restaurants and tagged Calistoga Cafe. Bookmark the permalink. 3 Comments.
Pingback: Perfect day « See Sarah Eat


text:
How to learn Graphic Design in Photoshop
Graphic design is like a visual design that give the original look to image or text means something appear like a real. Graphic design is most different or we can say unique creativity which is mostly appreciated by people, even people almost love to see everything graphical today is the time of graphics. If you want to be perfect in graphic designing then just need to take a short course and we provide here that awesome course at the reasonable prize. Now Graphic design field is a leading field among all other streams because it takes a short time to make you perfect and it also an interesting or creative field that's why people mostly like to choose a graphic design course.
The photo shop is only a way which makes everything very easier, so graphics mostly designed in the Photoshop have a hundred of elements which give the extraordinary look to the image. Here some steps for you.
- Opening a file and creating a new file
- Using Tools from Photoshop's Toolbar
Marquee Tools
2. Move tool
3. Lasso Tools
4. Magic Tool
5. Using the image menu
6. Layer Management
7. Working with Text
8. Creative Graphic
Graphic design now come in all the fields like today's time almost all games are well designed with the graphic design even film maker also like to give some graphical touch to their film. Everything which is graphically designed look too attractive and easy to understand. 21 century is a children century you can see today time children are extraordinary and very competitive they want everything graphically even they want to live a graphical life they like to imagine everything in the graphical form so graphical design is leading firm which is more popular everywhere.


text:
Rugged Gateways That Stay Connected
AirLink gateways can be deployed without any programming and are built with the intelligence to always stay connected. Relied upon every day in remote outdoor locations, in vehicle applications, and retail chains, they securely connect people, equipment, and services.
Intelligence That Accelerates Deployment
ALEOS® embedded intelligence is the power behind AirLink gateways that enables them to connect with industrial, enterprise, and mobile equipment out-of-the-box. Its rich set of configurable parameters simplifies device deployment and provides a reliable, standardized experience.
Programmability Without Any Embedded Expertise
The ALEOS Application Framework and integrated development environment make it easy to process data inside an AirLink gateway. Collect and analyze information from connected equipment and optimize data transfers using a simple, Lua-based scripting language. Now you can write embedded applications without deep embedded expertise.
Remote Management That Simplifies Operations
AirVantage® Management Service is a cloud application that simplifies and scales M2M operations, allowing you to manage a thousand devices as easy as ten. Deploy faster and reduce your operational costs by remotely configuring, deploying, and monitoring any number of AirLink devices over-the-air.
Cloud Platform To Access Your M2M Data
The AirVantage Enterprise Platform enables you to utilize data collected from AirLink devices to bring innovation to business processes, service applications, and revenue streams. Offering easy access to machine data using the latest web 2.0 API standards, our cloud platform simplifies the integration of wireless data with enterprise applications.
Why Sierra Wireless AirLink?


text:
By STAYWARE®
Perfect for all ages and abilities, these, slip-, tip-, & flip- resistant self feeding plates help minimize mealtime stresses. The reversible two-in-one patented design goes from partitioned to non partitioned with just a flip. Side A is divided with a spill guard and suction. Side B is non-skid and sloped with a side scoop wall. Simply press the center to make it stay, lift its edge to take it away. With no suction tab to grab or lip to flip, STAYnEAT plates stay firmly in place. The larger version suctions on both sides. STAYnEAT Jr. fits on most high chairs. The suction feature is designed for table tops only. A must have for picky eaters and individuals with special needs such as Autism, Alzheimers, Parkinson's, etc. These perfected all in one solution plates are a favorite feeding tool for all Occupational & Speech Therapists.


text:
Bone marrow mesenchymal stem cells (BM-MSCs) are an attractive cell based therapy in the treatment of CNS demyelinating diseases such as multiple sclerosis (MS). Preclinical studies demonstrate that BM-MSCs can effectively reduce clinical burden and enhance recovery in experimental autoimmune encephalomyelitis (EAE), a commonly used animal model of MS. However, a number of recent clinical trials have not shown significant functional benefit following BM-MSC infusion into MS patients. One possibility for the discrepancy between animal and human studies is the source of the cells, as recent studies suggest BM-MSCs from MS patients or animals with EAE lack reparative efficacy compared to naïve cells. We sought to define important transcriptional and functional differences between diseased and naïve MSCs.
We utilized RNA Sequencing (RNA-Seq) to assess changes in gene expression between BM-MSCs derived from EAE animals and those derived from healthy controls. We show that EAE alters the expression of a large number of genes in BM-MSCs and changes in gene expression are more pronounced in chronic versus acute disease. Bioinformatic analysis revealed extensive perturbations in BM-MSCs in pathways related to inflammation and the regulation of neural cell development. These changes suggest that signals from EAE derived BM-MSCs inhibit rather than enhance remyelination, and in-vitro studies showed that conditioned medium from EAE MSCs fails to support the development of mature oligodendrocytes, the myelinating cells of the CNS.
These data provide insight into the failure of autologous BM-MSCs to promote recovery in MS and support the concept of utilizing non-autologous MSCs in future clinical trials.
Mesenchymal stem cells (MSCs) are a multipotent class of stem cell with potential as a cell-based therapy for both autoimmune and neurological diseases (1). While MSCs can be derived from a variety of tissues, one of the most common and readily available sources of MSCs for clinical applications is the bone marrow (2). Bone marrow derived MSCs (BM-MSCs) are a non-hematopoietic type of stem cell that can be collected and expanded in-vitro from both human donors and experimental animal models (2).
BM-MSCs possess a remarkable capacity to modulate the responses of different immune and neural cell types (3). For example, BM-MSCs have strong immunosuppressive functionality (4), and can inhibit the proliferation and activation of a range of immune cells including T-cells, B-cells, and macrophages (1, 5). These immunosuppressive capabilities appear to be mediated in large part by the secretion of a broad spectrum of anti-inflammatory chemokines and cytokines by BM-MSCs (6). In addition to their immunomodulatory capabilities BM-MSCs are also neuroprotective and may influence the development of distinct classes of neural cells in the CNS. For example, BM-MSCs as well as conditioned medium from BM-MSCs biases neural stem cell fate away from an astroglial fate and promotes neural stem cell differentiation into oligodendrocytes and/or neurons (7, 8). In addition, BM-MSCs and BM-MSC conditioned medium enhances differentiation of oligodendrocyte precursor cells (OPCs) into mature oligodendrocytes (9). The effects of BM-MSCs on neural cells also appears to be mediated by secreted factors, including growth factors like hepatocyte growth factor (HGF) (10).
Due to their immunosuppressive and neuromodulatory properties, BM-MSCs are considered to be a potentially powerful cellular therapy for inflammatory and neurodegenerative diseases of the CNS such as multiple sclerosis (MS) (11). Experimental autoimmune encephalomyelitis (EAE) is a commonly used model of MS that results in a predictable onset of functional deficits that correlate with immune mediated CNS demyelination (12). When BM-MSCs are transplanted intravenously into EAE they rapidly halt disease progression and improve motor recovery and function (8, 13, 14). Although the mechanisms mediating this functional improvement are not well defined, transplanted BM-MSCs appear to suppress the immune response as well as the activation and migration of peripheral immune cells into the CNS of EAE mice (8, 13). In addition, BM-MSCs attenuate demyelination and possibly promote remyelination and axonal survival (8, 13, 15). The therapeutic benefits of BM-MSCs in EAE appear to be mediated by their secretion of anti-inflammatory and pro-regenerative factors (10, 16).
Based on their success in treating animal models of MS like EAE, BM-MSCs have moved into clinical trials for MS patients. The majority of these trials use autologous MSCs (derived from MS patients) (17), in contrast to most previous studies in EAE animal models, which used MSCs from healthy human donors or healthy animals ("naïve MSCs") (8, 13, 14). New evidence suggests however that inflammatory disease can alter the therapeutic functionality of BM-MSCs. In a recent study, BM-MSCs derived from EAE mice and MS patients were shown to have reduced therapeutic efficacy compared to naïve MSCs in their ability to ameliorate disease progression in EAE (18). The MSCs derived from donors with ongoing disease secreted higher levels of many pro-inflammatory cytokines, and demonstrated less immunosuppressive potential in-vitro (18).
Defining how diseases like MS affect the functionality of BM-MSCs is crucial in evaluating whether autologous MSCs represent a viable strategy for treating MS and other inflammatory diseases. The current study was undertaken to gain insight into how disease affects BM-MSCs at a transcriptional level though RNA-Sequencing experiments to compare gene expression profiles between naïve MSCs and MSCs derived from EAE mice (EAE-MSCs). Alternations in the expression levels of a large number of genes between EAE-MSCs and naïve MSCs were detected using this approach, with bioinformatic analysis revealing significant perturbations in pathways related to inflammation and immune cell regulation as well as myelination and neural cell fate. Such changes in gene expression in EAE-MSCs correlated with important functional consequences, as conditioned medium from EAE-MSCs failed to promote oligodendrogenesis in vitro and instead favored astroglial expansion. Collectively, this data underscores how inflammatory diseases like EAE dramatically alter BM-MSCs at a transcriptional and functional level, raising concern about the efficacy of using autologous MSCs as a cellular therapy for MS.
All animal experiments were performed according to protocols approved by Case Western Reserve University School of Medicine's IACUC with adherence to the NIH Guide for the Care and Use of Laboratory Animals.
EAE was induced in 10 week-old female C57BL/6 mice (Jackson Laboratory: 000664) using Hooke Labs MOG35–55 EAE Induction kits according to the manufacturer's protocol. Briefly, mice were immunized via subcutaneous injection of 200
Mesenchymal stem cells were isolated and cultured from the bone marrow of MOG35–55 – induced EAE mice at 14 days (peak EAE-MSCs) or 28 days (chronic EAE-MSCs) after immunization, with the animals having a clinical score of 4. Naïve MSCs were cultured from non-immunized, age-matched C57BL/6 mice. Growth medium for all cultures consisted of
BM-MSCs were isolated as previously described (18). Briefly, bone marrow from the tibias and fibulas was collected by flushing the central canal of the bone with a 26 ×g syringe containing fresh growth medium. Bone marrow cells were then seeded in P75 flasks (Corning) at a concentration of 2×105 cells/cm2 and grown at 37°C with 5% CO2. Flasks were washed twice with media 48 hours later to remove non-adherent cells, and the medium changed every 2~3 days. Cells were passaged using 0.25% Trypsin/EDTA (Gibco) for 2 minutes at 37°C and re-plated in P75 flasks at a concentration of 1×104 cells/cm2.
At the third passage (21 to 28 days in-vitro), 1×106 MSCs were collected for RNA extraction using RNeasy Mini kits (Qiagen) according to the manufacturer's protocol. An independent culture preparation consisting of MSCs derived from 2~3 mice was used for each RNA sample. Conditioned medium (CM) was likewise collected from passage 3 naïve and EAE-MSCs between 21 and 25 days in vitro, with unconditioned medium collected as a control. All CM samples were filtered through a 0.22
RNA libraries were prepared using TruSeq Stranded Total RNA Library Prep kits with Ribo-Zero Gold according to the manufacturer's protocol. Samples were analyzed for whole-transcriptome analysis using Illumina HiSeq 2500 v2 (RAPID RUN), with a read length of 1×50 bp for gene expression analysis. RNA-Seq data can be accessed through NCBI's Sequence Read Archive (https://www.ncbi.nlm.nih.gov/sra) with submission number SUB3074064.
Mapping of reads and differential expression analysis was done in BaseSpace using the RNA Express Legacy application (version 1.0.0). Reads were mapped to the
Gene ontology analysis of the top 2,000 differentially expressed genes (ranked by greatest magnitude fold change) was performed using DAVID (version 6.7). Further gene pathway analysis of all differentially expressed genes was performed using MetaCore. Heat maps were constructed using OriginLab Pro (version 2017) with genes mapped according to their respective z-score.
Dissociated neural cell cultures were prepared as previously described (8). Briefly, spinal cords from P3 C57/B6 mice were dissected manually and chemically dissociated in 0.1% Trypsin/EDTA for 20 minutes and passed through a 30
To test the effects of MSC CM of neural cell development and differentiation, cells were cultured in medium that contained 50% neural cell culture media and 50% MSC CM or unconditioned medium (controls) for 4 days. Coverslips were fixed for 10 minutes in 4% paraformaldehyde and labeled for respective cell markers using a previously published protocol (8). Antibodies used include: rat anti-MBP (Abcam: ab7349 at 1:100), rabbit anti-GFAP (Millipore: ab5804 at 1:800), and rabbit anti-NG2 (Millipore: ab5320 at 1:200). To quantify the percentage of cells labeled with each respective marker, 6 fields per coverslip were counted, with 2–3 coverslips per experiment over 2 independent experiments.
For RNA sequencing and expression experiments, statistical analysis was performed via the RNA express app in BaseSpace, with q-values (multiplicity adjusted p-values) of ≤0.05 considered statistically significant. For cell counting experiments, statistical tests were performed using GraphPad Prism 6, with Dunnett's multiple comparison tests performed post-hoc for one-way ANOVAs and p-values of ≤0.05 considered statistically significant.
RNA-Seq analysis was conducted on MSCs derived from the bone marrow of MOG35–55-induced EAE mice at two distinct phases of disease: at 14 days post induction during the peak of the disease ("peak EAE-MSCs") and at 28 days post induction during the chronic phase of the disease ("chronic EAE-MSCs"). These specific time points were selected based on a previous study showing BM-MSCs derived from MOG-induced EAE mice at these stages of disease have a diminished capacity to suppress inflammation and ameliorate EAE when compared to MSCs derived from non-diseased donors (18). To identify transcriptional changes underlying the reduced therapeutic functionality in EAE-MSCs, gene expression profiles of both peak and chronic EAE-MSCs were compared to expression profiles of naïve MSCs derived from healthy, sex-matched littermate controls.
To ensure that control MSCs were appropriately age-matched to their respective disease counterparts, different cultures of naïve MSCs were derived from healthy littermates at the time of peak EAE and two weeks later for chronic EAE. At least three biological replicates were used for gene expression comparisons and analysis. A different primary culture preparation, consisting of 1×106 BM-MSCs expanded for 3 passages (21~28 days in-vitro) and derived from 2~3 mice, was used for each replicate.
RNA-Seq analysis revealed widespread changes in gene expression patterns in EAE-MSCs that correlated with disease progression. Differentially expressed genes (DEGs) between EAE-MSCs and naïve MSCs were defined by a magnitude fold change of 1.5 or greater, q-value ≤0.05, and a normalized mean expression count ≥10. Using these criteria, RNA-Seq analysis identified 2,337 DEGs between peak EAE-MSCs and naïve MSCs (Fig. 1A). Chronic EAE-MSCs had more pronounced changes in gene expression, with 5, 837 DEGs between chronic EAE-MSCs and naïve MSCs (Fig. 1A). The magnitude of individual gene expression changes in chronic EAE-MSCs also tended to be higher than the magnitude of gene expression changes in peak EAE-MSCs (Fig. 1A). For example, when comparing chronic EAE-MSCs to naïve MSCs, 34% of DEGs showed a fold change of 3 or greater, whereas only 12% of DEGs showed a fold change of 3 or greater when comparing peak EAE-MSCs to naïve MSCs. Unsupervised hierarchical clustering showed peak EAE-MSC and chronic EAE-MSC replicates clustered together independently of naïve MSC replicates (Fig. 1B). These data indicate that both peak EAE-MSCs and chronic EAE-MSCs have distinguishable and reproducible changes in gene expression patterns compared to naïve MSCs.
The immunomodulatory and regenerative capacity of BM-MSCs in models of MS is derived in large part from their ability to secrete a wide array of diverse chemokines, cytokines, and trophic factors that may act on a act on a variety of cellular targets, including adaptive and innate immune cells involved in inflammation (6). To identify cellular and biological processes associated with the gene expression changes in EAE-MSCs, gene ontology (GO) enrichment analysis was performed on the DEGs found between naïve and EAE MSCs.
Gene ontology analysis suggested many DEGs are secreted factors or linked to the secretory pathway. For example, comparison of the distribution of DEGs amongst different cellular compartments revealed a large number of DEGs for both peak and chronic EAE-MSCs were associated with vesicles or the endoplasmic reticulum (ER) and Golgi apparatus (Fig. 2). Out of the top five specific cellular compartments DEGs mapped to, vesicles and the ER/Golgi apparatus were third and fourth respectively for both peak EAE-MSCs and chronic EAE-MSCs. The only compartments with a higher level of associated DEGs were the nucleus and cell membrane (Fig. 2).
Gene ontology enrichment analysis revealed many DEGs in EAE-MSCs were linked to immune activation and inflammation. For example, analysis of the DEGs for both peak EAE-MSCs and chronic EAE-MSCs showed that the third most enriched term (ranked by p-value) was "immune response" (Fig. 3A). Many of the GO terms associated with DEGs for both peak and chronic EAE-MSCs related to aspects of cell-mediated immunity or inflammatory response, including the terms "lymphocyte mediated immunity, "leukocyte chemotaxis", "positive regulation of immune response" and "activation of immune response." (Fig. 3A).
RNA-Seq analysis revealed an up-regulation of many immune and pro-inflammatory genes in EAE-MSCs compared to naïve MSCs. These included a number of inflammatory chemokines, cytokines, and other factors previously shown to be important in mediating MSC immunomodulation. For example, peak and chronic EAE-MSCs had elevated gene expression of multiple pro-inflammatory chemokines including Cxcl1 and Cxcl5, as well as interleukins important in T-cell activation such as IL-6 (Fig. 3B) (19). This data is consistent with a previous study showing that EAE-MSCs express higher amounts of each of these pro-inflammatory cytokines at the protein level and secrete higher levels of these factors compared to naïve MSCs (18). Previous studies have demonstrated that MSC-induced activation of the complement system after transplantation may reduce MSC viability and compromise their therapeutic efficacy (20). RNA-Seq analysis showed higher gene expression of many complement system factors in EAE-MSCs compared to naïve MSCs, suggesting enhanced complement activation by EAE-MSCs (Fig. 3B). The therapeutic efficacy of BM-MSCs is also influenced by Toll-like receptor (TLR) signaling that can affect the immunomodulatory characteristics of MSCs (21). The expression of many TLR genes was increased in EAE-MSCs compared to naïve MSCs (Fig. 3B), which may contribute to the functional differences between the two cell populations.
Further analysis of DEGs was performed using MetaCore to identify specific biological pathways associated with the gene expression changes observed in EAE-MSCs. MetaCore analysis revealed many DEGs in EAE-MSCs were associated with pathways important in immune cell signaling and immune response. For example, gene expression changes in peak EAE-MSCs and chronic EAE-MSCs were linked to the regulation of T-cell differentiation and activation, and to signaling pathways important in T-cell polarization (Fig. 4A). These results are consistent with previous findings from GO enrichment analysis of the DEGs in EAE-MSCs that found immune and inflammatory processes were significantly over-represented (Fig. 3A). Together the comparative analysis of gene expression between naïve and EAE BM-MSCs reveals an extensive change in immune associated genes. This is consistent with the notion that EAE-MSCs are more pro-inflammatory than naïve MSCs and have reduced immunosuppressive functionality compared to naïve MSCs both in-vitro and in-vivo after transplantation into EAE mice (18).
In addition to being immunomodulatory, previous studies have suggested that naïve MSCs have the capacity to influence neural cell fate (7–9). Metacore analysis of the DEGs between EAE-MSCs and naïve MSCs identified changes in pathways important in regulating neural cell development. In both peak and chronic EAE-MSCs, multiple DEGs were associated with "oligodendrocyte differentiation," and expression changes in peak or chronic EAE-MSCs were linked to "neural stem cell lineage commitment," "astrocyte differentiation," and "neurogenesis" (Fig. 4A). Signaling pathways important in neural cell differentiation and development, including WNT, EGFR, and PDGF signaling (22), were also associated with gene expression changes in peak and chronic EAE-MSCs (Fig. 4A).
EAE-MSCs showed changes in gene expression for multiple factors known to regulate neural cell development and oligodendrocyte differentiation. For example, both peak EAE-MSCs and chronic EAE-MSCs up-regulated expression of Fgf and Bmp genes, including Fgf2, Bmp4, and Bmp6 (Fig. 4B). FGF and BMP proteins are important in influencing neural cell fate, as they favor the differentiation of neural stem cells (NSCs) and oligodendrocyte precursor cells (OPCs) into astrocytes versus their differentiation into oligodendrocytes (23, 24). Chronic EAE-MSCs also down regulated expression of genes like Tgfb1 and Igf1, which code for growth factors that promote oligodendrocyte maturation and differentiation (25, 26).
Such changes in gene expression suggest EAE-MSCs may differentially influence neural cell fate compared to naïve MSCs. To test this hypothesis, dissociated cultures of spinal cord neural cells were treated with conditioned medium (CM) from naïve MSCs or EAE-MSCs. Cultures were treated for 4 days (with unconditioned medium used as a control) and the proportion of GFAP+ astrocytes and MBP+ oligodendrocytes was assayed to determine whether factors secreted by EAE-MSCs differentially bias neural cell development.
While cultures grown in CM from naïve MSCs had increased numbers of mature oligodendrocyte compared to controls, cultures grown in CM from chronic EAE-MSCs had reduced numbers of mature oligodendrocytes and increased numbers of astrocytes (Fig. 4C). Parallel studies using CM from Peak EAE-MSCs showed a less significant effect with a slight increase in the number of astrocytes and no reduction in the number of oligodendrocytes (Fig. 4C). In both cultures the presence of EAE-MSC CM increased astrocyte reactivity resulting in elevated expression of GFAP and a more dense network of processes. Changes in the relative number of oligodendrocytes in cultures treated with CM from chronic EAE-MSCs appeared to reflect changes in oligodendrocyte maturation since no significant effect on the number of NG2+ oligodendrocyte precursors was seen compared to controls (Fig. 4C). No changes were seen in the relative number of neurons or microglial cells, which at less than 5% each accounted for a very small percentage of cultured cells (data not shown). These data suggest that the failure of EAE-MSCs to promote recovery in animal models of CNS demyelination may be associated in part with their reduced capacity to promote the development of mature oligodendrocytes.
RNA-Seq analysis suggests that gene expression changes in BM-MSCs during EAE evolve with disease progression. For example, the number of DEGs is much higher when comparing chronic EAE-MSCs to naïve MSCs versus comparing peak EAE-MSCs to naïve MSCs. To investigate what genes are differentially expressed between peak and chronic EAE-MSCs, expression profiles of peak EAE-MSCs and chronic EAE-MSCs were compared to each other using previous criteria for DEGs: magnitude fold change of 1.5 or greater, q-value ≤0.05, and a normalized mean expression count ≥10.
RNA-Seq analysis revealed BM-MSCs show continual changes in gene expression patterns throughout EAE progression. There were 3,736 DEGs between peak EAE-MSCs and chronic EAE-MSCs (Fig. 5A). Consistent with comparative studies between naïve and EAE-MSCs, GO analysis identified many of these genes as being associated with immune or inflammatory processes (Fig. 5B). Also, GO analysis suggested many of these DEGs are associated with processes involved in cell uptake and secretion, as "phagocytosis," "membrane invagination," and "endocytosis" were among top GO terms (Fig. 5B).
Many of the DEGs found between peak and chronic EAE-MSCs were immune or inflammatory genes. These include some of the same chemokine and cytokine genes previously found elevated in EAE-MSCs in comparison to naïve MSCs. For example, chronic EAE-MSCs had elevated levels of expression of pro-inflammatory cytokine genes such as Cxcl1, Cxcl5, and Cx3cl1 compared to peak EAE-MSCs (Fig. 5C). Not all gene expression was elevated in chronic EAE-MScs. Several Toll-like receptor genes and compliment component genes were down regulated in chronic EAE-MSCs compared to naïve MSCs (Fig. 5C).
Overall, there was considerable consistency in the genes whose expression changed in BM-MSCs during EAE. For example, of the 2,337 DEGs identified between peak EAE-MSCs and naïve MSCs, 65% of these (1,498/2,337) were also differentially expressed between chronic EAE-MSCs and naïve MSCs (Fig. 5D). Furthermore, of the 5,837 DEGS found between chronic EAE-MSCs and naïve MSCs, 80% of these genes (2,979/3,736) were differentially expressed when comparing peak EAE-MSCs to chronic EAE-MSCs. In total, there were 988 common DEGs in BM-MSCs throughout all time points analyzed during EAE (Fig. 5D).
Bioinformatic analysis revealed a high degree of conservation in the transcriptional regulators associated with the DEGs identified in EAE-MSCs. For example, the transcription factor SP1 was most significantly associated with the DEGs identified in both peak EAE and chronic EAE-MSCs, and both peak EAE-MSCs and chronic EAE-MSCs showed a high degree of similarity in other transcription factors linked to their respective DEGs (Fig. 5E). Overall the current bioinformatic analysis suggests the changes in gene expression by BM-MSCs during EAE and the transcriptional regulators associated with these changes are highly conserved.
Utilizing RNA-Sequencing, genome-wide expression profiles of BM-MSCs derived from healthy and EAE mice have been analyzed and compared for the first time to provide a better understanding of the transcriptional and functional changes BM-MSCs undergo during inflammatory disease. Widespread gene expression changes were detected by RNA-Seq analysis in BM-MSCs derived from animals with EAE compared to naive cells derived from non-diseased animals. The degree of altered gene expression correlated with disease progression. Many of the most pronounced increases in gene expression were in genes involved in the regulation of the immune system and inflammatory processes. Bioinformatic analysis also identified important changes in EAE-MSCs in genes associated with pathways that influence neural cell fate and antagonize oligodendrocyte differentiation. Consistent with these changes in gene expression, conditioned medium from EAE BM-MSCs reduced the number of mature oligodendrocytes that developed in cultures of neonatal spinal cord, while conditioned medium from naïve BM-MSCs increased the number of oligodendrocytes in parallel cultures.
Mesenchymal stem cells have many characteristics that make them suitable for clinical application in a number of diseases (27). In order to ensure maximal benefit of such trials it is important that the most appropriate cells are used and the cells should be defined as effectively as possibly. The gene expression changes identified between naive BM-MSCs and MSCs derived from an animal model of multiple sclerosis (EAE) are potentially important given the majority of clinical trials for MS patients use autologous MSCs that are likely more equivalent to EAE derived MSCs. While preliminary results from these clinical trials report good safety data in transplanting autologous MSCs into MS patients, most report limited therapeutic efficacy (28–31) prompting questions about whether autologous MSCs from MS patients (MS-MSCs) are equivalent to allogeneic MSCs from healthy patients (naïve MSCs) in their functional characteristics and therapeutic potential (32).
Comparisons of the biological and functional characteristics of naïve and MS- MSCs have failed to reach consensus as to whether MS-MSCs show differences in characteristics like proliferation or cytokines secretion compared to naïve MSCs (33–35). One recent study reported MS-MSCs have less immunosuppressive functionality compared to naïve MSCs in-vitro, and utilized microarrays to profile and compare gene expression in MS-MSCs and naïve MSCs (36). Although there was a significant up-regulation of inflammatory genes in MS-MSCs, consistent with the RNA-Seq analysis of EAE-MSCs in the current study, the magnitude of changes was more limited. For example, less than 700 DEGs were seen in the MS study, compared to the greater than 2,000 DEGs seen in EAE (36). Interpreting genomic or functional data that compares MS-MSCs to naïve MSCs can be complicated as there are a range of variable that may alter the data and are difficult to control for. Factors such as genetic background or donor age may influence the functionality of BM-MSCs (37). Likewise, current or previous medication regimes, disease duration, and disease severity may affect gene expression levels in autologous BM-MSCs. Together such variables may make defining significant functional or gene expression changes in BM-MSCs derived from patients with diseases such as MS difficult. The utilization of a mouse model of disease such as EAE eliminates many of these variables, including genetic traits and prior disease history, and can provide clearer insight into the effects of disease on BM-MSCs.
A recent study comparing BM-MSCs derived from MOG EAE mice to naïve MSCs from healthy, age-matched mice demonstrated that the EAE-MSCs had a reduced ability to ameliorate functional deficits caused by EAE (18). This lack of therapeutic efficacy of EAE-MSCs was correlated with increases in the relative levels of pro-inflammatory cytokines and decreases in the levels of anti-inflammatory growth factors EAE-MSCs secreted relative to naïve MSCs. This cytokine expression data is consistent with the present RNA-Seq analysis showing EAE-MSCs up-regulate a wide variety of inflammatory genes associated with immune response and immune cell activation. The up regulation of gene expression for Cxcl1, Cxcl5, and IL-6 correlates directly with the increased expression of these cytokines in conditioned medium from EAE-MSCs (18).
The duration of disease appears to directly influence BM-MSC gene expression. For example, the number of DEGs was significantly increased in chronic EAE-MSCs versus naïve MSCs, and the magnitude of the gene expression changes tended to be higher in chronic EAE-MSCs compared to naïve MSCs. Increases in gene expression were particularly evident with inflammatory genes. These results are consistent with previous findings that BM-MSCs isolated from MOG-induced EAE mice during the chronic phase of the disease express elevated levels of inflammatory cytokines and have less immunosuppressive potential than BM-MSCs isolated from EAE mice during the peak of the disease (18).
In addition to modulating the immune system, MSCs have been suggested to influence neural cell fate. Several studies demonstrate that naïve MSCs have the capacity to enhance oligodendrocyte differentiation and maturation in-vitro and remyelination in-vivo in demyelinating animal models (8–10). It is currently unclear whether MSCs alter the fate of multipotent neural progenitor cells or directly promote the differentiation of oligodendrocyte precursors when transplanted in-vivo. Furthermore, the relative contributions of MSC mediated recovery in EAE from their capacity to influence neural cell fate and remyelination versus the ability of MSCs to suppress inflammation and the immune response is currently unclear (38). RNA-Seq analysis revealed a significant up regulation in gene expression of a number of TGF
Until there is a better understanding of how MS impacts the therapeutic potential of BM-MSCs, careful consideration should be given in using allogenic versus autologous MSCs in clinical applications to treat MS. Future studies exploring ex-vivo manipulations that could enhance or restore the therapeutic functionality of autologous MS-MSCs such as differential culture conditions or modification of gene expression may provide a more acceptable approach to an effective therapy. The present RNA-Seq analysis of EAE MSCs provides new pathways and potential targets that could be exploited to achieve this effect.
RNA-Seq analysis of BM-MSCs in EAE helps to support newly emerging evidence that disease alters the functional characteristics and therapeutic capacity of MSCs. EAE-MSCs show clear changes in gene expression patterns relative to naïve MSCs, including up-regulation of inflammatory genes. In addition, EAE-MSCs differentially modulate neural cell development and fail to promote the formation of mature oligodendrocytes in-vitro. We show that inflammatory disease dramatically alters BM-MSCs at both a transcriptional and functional level, raising concern about the efficacy of using autologous MSCs to treat MS.
This work was supported by NS30800 (NIH) and the Myelin Repair Foundation. The authors' thank the Case Western Reserve University Genomics Core for RNA library preparation and sequencing services and the George Washington University School of Medicine and Health Sciences McCormick Center for Genomic and Proteomic Research for assistance with data analysis.
The authors have no conflicting financial interest.


text:
09 Juli 2014
The headquarters building of Royal Thai Navy's Submarine Squadron (photo : TAF)
The Royal Thai Navy's Submarine Squadron was officially launched at Sattahip naval base in Chon Buri province yesterday. Navy chief ADM Narong Pipattanasai presided over the ceremony.
The squadron also has a training centre for a submarine command team, which is intended to compile knowledge on submarine operations. The centre, which is equipped with a German-made submarine simulator, provides navy personnel with training, such as how to operate the sonar system.
The submarine simulator is also linked to the anti-submarine simulator currently operated by the navy's Fleet Training Command.
The navy has also sent 18 navy officers to receive training in submarine technology in Germany and another 10 officers to South Korea to attend a training course on international diesel submarines.
The squadron underscores the Royal Thai Navy's push to get submarines added to its fleet. The navy has pressed governments for years to buy submarines to help navy ships protect the Gulf of Thailand.
In 2010, then navy commander Kamthorn Phumhiran approved a plan to set up the squadron, with expectations of having second-hand submarines from Germany. But it never passed the planning stage.
In 2012, then navy chief Surasak Rounroengrom approved guidelines for equipping navy personnel with training on submarines, which led to navy personnel studying submarines with the help of countries who have had them for a long time.
"Although the navy has not had submarines for 62 years, submarines are still important as a strategic and irreplaceable tool to make up for the geographical limitations of Thailand,'' the navy said in an article to promote the underwater craft.
(Bangkok Post)
09 Juli 2014
Navy Launches Submarine Squadron
Label: ANGKATAN LAUT, Kapal Selam, THAILAND
Langganan: Posting Komentar (Atom)


text:
U.S. News & World Report recognized a range of graduate programs at UL Lafayette in its 2021 Best Graduate Schools national rankings.
Blanco to speak, receive honorary doctorate at CommencementMon, 12/11/2017 - 9:31am
UL Lafayette alum and former Louisiana Gov. Kathleen Babineaux Blanco will be guest speaker at the University of Louisiana at Lafayette's Fall 2017 Commencement ceremony on Friday.
She also will receive an honorary doctorate.
The General Assembly will be held at 11 a.m. at the Cajundome.
Blanco was governor from 2004 to 2008.
The year after she took office, Blanco led the state when Hurricanes Katrina and Rita struck the state.
During her term, she directed the largest investment increase in higher education in state history. Blanco expanded the state's pre-K program and fully funded Louisiana's colleges and universities for the first time in 25 years.
She was a two-term lieutenant governor. Blanco was first elected in 1995 and reelected in 1999.
As lieutenant governor, she oversaw the Department of Culture, Recreation and Tourism. State tourism increased by 41 percent under her leadership. The industry's growth resulted in a $2.5 billion increase to the state economy and the creation of 121,000 tourism-related jobs.
In 1984, Blanco was elected to the state House of Representatives. Five years later, she was the first woman elected to the state Public Service Commission. Blanco was commission chairperson in 1993 and 1994.
Earlier in her career, she was a political consultant, a district manager for the U.S. Census and a high school teacher.
Blanco earned a bachelor's degree in business education from the University in 1964.
She is married to Raymond Blanco. He retired as UL Lafayette's vice president for Student Affairs in 2008 after serving in many roles during 46 years at the University.
Learn more about Fall 2017 Commencement at commencement.louisiana.edu.


text:
U of M student start-ups win 2015 Acara Challenge with innovative impact ventures
Student-run impact ventures focused on solar-powered microgrids for rural India and environmentally friendly feminine hygiene products have been selected Gold Level winners of the 2015 Acara Challenge, a competition held by the University of Minnesota's Institute on the Environment in partnership with the College of Science and Engineering and the Carlson School of Management. The top-level teams and other awardees will have the opportunity and resources to further develop their innovative business solutions for environmental and social challenges.
The challenge was divided into a domestic and an international division, each with gold, silver, and bronze levels. Gold Level winners will receive $1,000 toward pilot expenses. Silver Level honorees will receive $750 and Bronze Level recipients get $500. Winning teams on all three levels also receive individual scholarships to the Acara Spring Institute in St. Paul or Summer Institute in Bangalore, India. Select ventures are also invited to receive up to $5,000 in additional funding to pilot their idea.
"We are very impressed with the creativity and commitment to change embodied in these projects and glad to have the opportunity with the help of our generous funders to incubate the winning start-ups over the coming year as they develop their venture plans into operational social enterprises, generating revenue and impact in Minnesota and abroad," said Acara co-director Fred Rose of the Institute on the Environment.
"These students are passionate about launching new ideas to have a positive impact on the world; we are pleased to be able to recognize and support their ideas," said Acara co-director Julian Marshall, a professor in the College of Science and Engineering.
The Acara Challenge, which began in 2009, spurs start-ups with creative, sustainable solutions that can have an impact in the real world.
The 2015 Acara Challenge winners are:
Domestic Division
Ova Woman
This online retail and marketing company sells menstrual cups and fashionable absorbent underwear, helping to reduce the burden of feminine hygiene products on landfills. The initiative is led by Elise Maxwell, a student in the Carlson School of Management.
City Compost MN*
This Minneapolis waste processing company cuts trash volume and creates a valuable end product that is missed by current systems. The team is led by Peter Schmitt, a student pursuing a dual degree from the Carlson School of Management and the Humphrey School of Public Affairs, and Katie Schmitt.
*Crowd Favorite – Domestic Division
For marginalized job seekers who need career experience, Autonomee is TaskRabbit for software development. The initiative is led by Steven Bruce, a student in the Carlson School of Management.
InCOLOR Magazine
This online multimedia magazine is designed to serve as a voice for the various multicultural and inner-city communities. The initiative is led by Tiffany Trawick, a student from the College of Liberal Arts.
Minnetonka Local
This venture offers business development and retail support to encourage lower-income entrepreneurs, increasing local products and services while building community engagement. The team is led by Alana Buckner, a student in the Carlson School of Management.
Honorable Mentions
The BDW Blog
With an aim to close the gap between public option and scientific consensus, the BDW Blog provides a neutral, impartial voice. The team includes students Adam Woodruff (College of Science and Engineering), Yogesh Dhande (College of Science and Engineering), Josh Magnuson (Carlson School of Management), Joanna Mooney (College of Biological Sciences) and Thomas Harris (Bowling Green State University).
Knack is a Web-based business that seeks to tap the potential of young adults to address social issues affecting the Midwest through local artisan product sales. The team includes students Taisha Bauer, Francesca Berarducci and Megan Zimmerman from the University of Minnesota, Duluth.
International Division
Stimulight seeks to improve the quality of life in rural India through the use of clean and reliable LED lights driven by solar-powered microgrids in place of kerosene lamps. The team includes students Robin Walz and Vicky Ong from the College of Science and Engineering.
Apis Krishi*
This venture aims to help rural Indian farmers leave the cycle of poverty by promoting beekeeping education and removing the financial risk of beekeeping. The team is led by student Erin Kayser from the College of Science and Engineering.
*Crowd favorite – International Division
This business created an accessible collection service to help citizens of Bangalore responsibly recycle e-waste. The team includes students Claire Warren (College of Science and Engineering), Aika Mengi (Humphrey School of Public Affairs), Joshua Auerbach (Carlson School of Management) and Malcolm Smith (College of Science and Engineering).
Ripple helps water purification and testing companies connect their products and services with rural markets to improve health in rural communities. The team is composed of students Anna Schulte (Carlson School of Management), Emma Volbrecht (College of Science and Engineering) and Adam Iversen (College of Science and Engineering).
The University of Minnesota's Institute on the Environment seeks lasting solutions to Earth's biggest challenges through research, partnerships and leadership development. For more information on IonE, visit environment.umn.edu. For more information on Acara, see acara.environment.umn.edu.


text:
You will need to sign in or create an account before you can use your wishlist…
Unleash your sensuous side with the Nesti Dante Romantica Cherry Blossom and Basil Soap, a vibrant celebration of Tuscany's most exotic fragrance sensations.
Cleanse and condition your skin with the uplifting partnership of delicate cherry blossom and invigorating basil. When combined, this powerful duo transports your senses to place of serenity with their harmonious flair and romantic allure.
Crafted with a unique saponification process, the bar is enriched with whole neutral vegetable fats and raw materials, which infuse your skin with sumptuous softening extracts whilst delicately scenting your skin with the delightful aroma of cherry blossom and basil.
Work into wet hands then massage the soap bar to create a sumptuous lather. Massage all over your body, then rinse. Keep soap in a clean, dry place after use to preserve its longevity.
100% biodegradable. Dermatologist tested. Not tested on animals.
Shipping Restriction: Please note that at the request of Nesti Dante we are not able to ship this product to the USA.
It looks like you are visiting us from The United Kingdom.
Please choose your delivery country and currency below.


text:
Penultimate gives you the fast, tactile gratification of writing on paper, with digital power and flexibility. Take notes, keep sketches, or share your next breakthrough idea -- in the office, on the go, or home on the sofa.
Penultimate stands with the core productivity apps on your iPad. Every detail of the user interface has been designed and refined to be simple and intuitive - no complicated or cluttered settings.
When you first open your new copy of Penultimate, you'll find a "welcome" notebook that will walk you through all of the major features. You'll be scribbling and whizzing through your pages and notebooks in no time.
Your handwriting and drawing looks spectacular in bold gel ink. Our writing technology is second to none. Use colors or stick with classic inky black. Three different photorealistic paper styles are included, along with a magically effective eraser tool that's always the right size. Want a special paper style? Import any image to create and share your own attractive template, or visit The Paper Shop in the app to see the latest convenient collections available for download.
Store your thoughts in named notebooks for each topic, project, or category. Penultimate can manage as many notebooks as you can create, each with an infinite number of pages. View your list in a notebook carousel -- or sort and scroll in a fast grid view. When your notebooks get large, you can browse quickly through all pages, or insert, delete, duplicate, and reorganize pages within and between notebooks.
Beneath its graceful exterior Penultimate is a powerful application. Our superior inking technology produces pen strokes that look better-than-real. The pioneering automatic Wrist Protection mode prevents most stray marks from appearing on a page for writers of all grips. Image import allows photo annotation, cataloging, and design work. Powerful ink selection and copy/paste is a touch away. +


text:
Flexform Groundpiece is simply a great sofa; it is wonderfully comfortable and versatile which enables you to create your own sofa using only the sections you want.
There are optional leather bookshelf armrests which lend function and style, as illustrated.
Groundpiece comprises a clever collection of seating elements and cushions in a variety of sizes. Available with goose-down or Dacron filling.
Down-filled sofa in fabric cat. Lusso, length 248cm including fabric armrests and 2 large roll cushions, from:
Dimensions: W: 248cm, D: 97cm, H: 56cm


text:
- Description
- Tasting Notes
- What's Inside
- Occasions
Give the gift of a splendid spring day to a special friend! This pretty mix of blooming flowers in gentle, pastel shades is just the thing to show you care.
Our floral artists arrange pretty blossoms such as spray roses, alstroemeria, cushion spray chyrsanthemums, carnations, and salal in a clear glass vase. Finished with a satin bow, the bouquet will add light and fragrance to any room.
Gift Includes:
- Pink Spray Roses.
- Pink Alstroemeria.
- Lavender Cushion Spray Chrysanthemums.
- Yellow Carnations.
- Assorted Lush Greenery and more.
- A free gift card with personal message.
- Arrives in a stylish glass vase with fresh water, ready to be displayed.
- Hand delivered to the recipient's home or office by experienced florists.
- A plush teddy bear, balloons, wine, champagne, or chocolates may be added to your order based on availability. Please contact customer service for more information.
Measures approximately 9" x 13".
During checkout, please be sure to indicate your preferred occasion when you choose a gift card. Exact flowers and vase may vary depending upon location and availability.
Same day delivery is available if you place your order by 2pm in your recipient's time zone, M-F and by 1pm on Saturday. To see if Sunday delivery is available in your area please contact customer service.Gift #7612
Splendid Spring Bouquet is a hand delivered gift. These gifts are delivered by one of our local design centers located throughout the United States and Canada.
Typically, standard deliveries are made between 9:00 AM and 6:00 PM, with business deliveries before 5:00 PM. Hours may be extended during holidays. Please note in some locations we may need an additional 24 hours for the delivery of exotic flowers, wine and fruit baskets.
Same day delivery is available for most gifts when ordered before the cutoff time. Saturday delivery is possible if ordered before 12 PM Friday. The cutoff time for Splendid Spring Bouquet is in the recipient's time zone.
Splendid Spring Bouquet has been purchased most frequently by our customers for the following occasions:
Average Rating:(6 reviews) write a review
Read More Reviews
Flower lover...
My friend that received the arrangement just said they were awesome... She just loved them! Thank you! You never let me down!
Lyns_MacGreat Customer Support!July 29, 2014
I ordered this gift from the UK and did not realize I had sent it to an incorrect address. The customer services rep worked tirelessly to track down the correct address and ensure the flowers were delivered in top condition. Great service, lovely flowers - can definitely recommend!
WendyGreat bouquet for this price!July 28, 2014
I was looking for a "budget friendly" condolence bouquet and I found this arrangement; flowers and color combination says it all... not too sad but it does the job... delivered on time and lifts up someone's spirit despite their loss!
ariosGORGEOUS FLOWERSMay 22, 2013
My mother-in-law loved them! :))))
jeepingGreat Customer ServiceMarch 17, 2013
NitaPassion FlowerNovember 6, 2011
the recipient of the flowers was so suprised and delighted. This not the first time I've sent a gift from your company. The recipent raves about how beautiful the gifts received are. I ve seen the products and it's always the best of quality.


text:
Text Added:
(Note: The text below was slightly modified on 5/16 after this post was originally posted.)
Serious Side Effects of This Entire Class of DrugsThe FDA issued a warning on 5/15/2015 stating that it had received a significant number of aftermarket reports linking this class of drugs to ketoacidosis. Ketoacidosis is a very serious, potentially fatal condition where the acid level in the blood rises dangerously high. Ketoacidosis makes people very sick and if untreated can be fatal. It requries a trip to the emergency room. Symptoms of ketoacidosis include: difficulty breathing, nausea, vomiting, abdominal pain, confusion, and unusual fatigue or sleepiness.
What is particularly worrisome here is that ketoacidosis usually ONLY occurs in people with Type 1 diabetes who have very high blood sugars--those above 300 mg/dl. However, in these cases the ketoacidosis was occuring in people with Type 2 diabetes who had only modestly elevated blood sugars. It is likely that these drugs are promoting ketoacidosis because of the way they affect the kidneys, which may make it harder for the body to eliminate ketones as they build up.
Typically, people eating very low carb diets are told that as long as their blood sugar isn't high, it's perfectly safe to have raised ketone levels in blood and/or urine. But this may NOT be the case if you are taking one of these drugs because they may block the normal processes that keep ketone levels within a safe range. Until it is 100% clear what is leading to ketoacidosis in people taking these drugs it is not a good idea not to take them if you are eating a ketogenic low carb diet. You can read the entire FDA warning at:
FDA Drug Safety Communication: FDA warns that SGLT2 inhibitors for diabetes may result in a serious condition of too much acid in the blood


text:
Product information:
Model No: WSWPII-12101
Age Group: 2-12
Capacity: 16
Structure Size: 770×620×450cm
Use Zone: 1140×900cm
Color options shown in this configuration are:
See inside back cover for more color options.
Send Inquiry
If you have any enquiry about quotation or cooperation,please feel free to email us at firstname.lastname@example.com or use the following enquiry form. Our sales representative will contact you within 24 hours. Thank you for your interest in our products.
- Subject:
- *Name:
- *Email:
- Company Name:
- Tel:
- Fax:
- Country:
- Products:
- Message:
Related Products


text:
In Georgia, there are approximately 200,000 people with Alzheimer's and the population of caregivers more than doubles that number. Alzheimer's disease is a form of dementia that gradually deteriorates the function of the brain. Although there is no cure for Alzheimer's, there are ongoing efforts to help people who have the disease and their loved ones to cope.
The Alzheimer's Association - Georgia Chapter is one organization whose goal is to raise awareness of the disease. Since 1982, the Georgia Chapter has served thousands of people, and continues to provide a number of services designed to educate and uplift their community. From spearheading educational programs and research efforts, to providing phone support services and fundraisers, the organization has established itself as a valuable resource for many Georgia residents affected by Alzheimer's.
Leslie H. Anderson, President and CEO of the Georgia Chapter, says that there are a variety of opportunities to get involved. The organization hosts events such as the Walk to End Alzheimer's and golf tournaments. Each year, Alzheimer's Awareness Day is hosted at the state capitol and there are many volunteer activities available.
In June, the organization will host the Dancing Stars of Atlanta fundraising competition, a spin-off of the "Dancing With the Stars" television show. The idea behind the fundraiser is for each dancer to reach a goal of $10,000. In 2010, event participants ranged from CEOs to professional athletes and politicians. "It's a unique way for us to celebrate the people who support us and support those who need us," Anderson says.
Today, the Alzheimer's Association - Georgia Chapter is involved in research surrounding early detection of the disease. In January, the National Alzheimer's Project Act was passed, which Anderson says is an historic legislative victory for the Alzheimer community.
The NAPA will allow supporters to help coordinate efforts to evaluate and overcome the effects of Alzheimer's through community based programs and research. Alongside their ongoing support of Alzheimer's advocacy on the national level, the Georgia Chapter plans to carry out a plan of its own. Through accelerated research, educational efforts and the growing revenue, the Georgia Chapter plans to continue their efforts to fight the disease. "The tsunami that is Alzheimer's disease is here and we simply must be ready for it," says Anderson.
Know the 10 Signs - Early Detection of Alzheimers's
- Memory changes that disrupt daily life
- Challenges in planning or solving problems
- Difficulty completing familiar tasks at home, at work or at leisure
- Confusion with time or place
- Trouble understanding visual images and spatial relationships
- New problems with words in speaking or writing
- Misplacing things and losing the ability to retrace steps
- Decreased or poor judgment
- Withdrawal from work or social activities
- Changes in mood and personality
If you have questions about any of these warning signs, the Alzheimer's Association recommends consulting a physician. Early diagnosis provides the best opportunities for treatment, support and future planning.
Courtesy of www.alz.org


text:
A man said he was at a residence getting high when three men held him up in an armed robbery early Thursday morning.
A man said he was at a residence getting high when three men held him up in an armed robbery early Thursday morning.
�The call was to the Lunch Box,� said Woody Spencer, spokesman for the Kinston Department of Public Safety. �When the officers got to Lunch Box they found the victim. The victim told them he was in Simon Bright smoking dope when he got robbed. He said he ran from Simon Bright to the Lunch Box, which � is on Martin Luther King, to get away from the subjects.
�(The narrative showed) the victim was out of breath, having trouble breathing.�
After a while, the victim got sick and couldn�t communicate to the officer and was taken to Lenoir Memorial Hospital for treatment.
Ultimately, officers were unable to verify the claim or make an arrest.
�But, due to lack of evidence, there were no arrests made, because (the reporting officer) could no longer talk to the victim, and they did find the two suspects in the Simon Bright area, but they were not out of breath,� Spencer said. �They didn�t seem like they were sweating or anything out of the ordinary, like if they were chasing somebody.�
Wes Wolfe can be reached at 252-559-1075 and email@example.com. Follow him on Twitter @WolfeReports.


text:
Spring break! Well it's not spring in where I live, but who cares. That thumbnail picture for this post as you can see, is a park located in central Jakarta. The picture was taken yesterday, after the rain on Saturday afternoon at Taman Suropati. I went there by myself enjoying the atmosphere, the smell of the wet land. Then I took a 15 minutes walk to Stasiun Sudirman, walking under the big trees in Menteng, and passing some cute unadopted puppies at Latuharhary street. So even though it's not spring here, the transition between rainy to dry season is also good. But of course I want to feel the real spring too, especially in Paris? :p Well anyway, today I decided to share some of my favourite songs which I've been listening to for the past weeks, and also.. a radio, and an awesome cover I found in Youtube! This is gonna be cool.
Before I begin, I wanted to tell you that the list is not ordered from least best to best or vice versa. It's totally all the songs I love. I also link each songs to iTunes so you can listen or maybe buy them (while links on the pictures, will bring you to the image source). Sooo here we go~
1. Uptown Funk // Mark Ronson feat. Bruno Mars
I started to really like this song a couple of months ago. I went out karaoke-ing with by best friends singing and dancing to this song and I think it was the best song we played at that time. Now I can't stop listening to it.
2. Wasting My Young Years // London Grammar
London Grammar is a British trio formed by Hannah Reid, Dan Rothman and Dominic 'Dot' Major. The band is into pop/electronica/indie genre, and originally formed in Nottingham, England, United Kingdom. Their debut EP Metal & Dust was released in February 2013 while their debut album If You Wait was released on September 2013. If You Wait has been certified platinum in Australia and the UK, shipping over 70,000 and 300,000 copies respectively (I got this facts from Wikipedia :P). Anyway, Wasting My Young Years is the first song I hear from London Grammar, I love to listen this song while working at the office <3 I like another song from them called Hey Now but this Wasting My Young Years is still my favourite. Oh and I just found out that the members are in their mid-twenties too just like me, I feel connected to them, lol. But seriously, I think I will listen to their other songs.
3. Got to Belong // Natas Loves You
Natas Love You is a band originated from France. Why do I love this song? Because this song is somehow so catchy and singable and different from other French songs that I listen to lately. I just love to listen to different kind of music! And I also found them playing this song on acoustic, it was so cool. You can watch it here https://www.youtube.com/watch?v=96NLUsAUMgw.
When you listen to the intro of this song, it's like you want to dance automatically, love it. Anyway, Emma Loiuse is a 21 year old singer-song writer from Australia. I know this song from a French radio, isn't that awesome.
5. R U Swimming // Mademoiselle K
It's a fast beat pop song, if I have to say. Listening to this song makes me sing and dance automatically. I think I'll listen to this song when going for a run. I also love to listen to the hit-hat sound this song creates. Coolness overload.
Random fact about Selah Sue is she was born in 1989, just like meeh <3 I love her eyes and her style. And of course this awesome song sang by her. If you keep reading this blog post, you'll find a link to an awesome remix of this song
7. I Bet My Life // Imagine Dragons
I think this song is about someone regretting their relationship with a person, how he neglected someone so important in his life that he's sorry for what he's done. Despite of its deep lyrics, I mostly love this song because when listening to it, it makes me want to ruunnn so fast especially in the chorus part lol.
Mabye you have listen to this song over and over, so do I. But this is such a great song, it's so nice to sing to. If you have seen the music video, you'll love this song more. You can see a man, leaves his daughter every morning to go to work. He wears a nice suit to make his daughter proud, but he changes it once he arrives at the workplace. Turns out he's just a garbage collector, an unconventional profession which is often underestimated by people. The video is so hurtful, he is such a superhero.
This is a kind of song you would love to hear when you're running. It's so catchy that you'll never get bored dancing to. I totally cannot stop listening to this song <3
10. Despair, Hangover & Ecstasy // The Dø
The Dø is a French/Finnish indie pop band founded a while ago, in 2005. This is the first song I listened to and it is so cool. I love this song more when watching them playing this song with another awesome French band but I won't spoiler about it, you'll read it in "best cover" section <3
This song is pretty different from other songs I mentioned earlier. It is a rap song. But it's still from French, I don't know what the song means, I just love French, and when it's a rap, I'll love it even more, lol. But of course, it's a catchy song too.
Gravity controls, gravity controls, the way we're falling
Gravity controls, gravity controls, the way we sink
We're floating into space, floating into space
So boy..
Why don't you kiss me?
And the last best song I listen to recently is.. GRAVITY. This song is played from a Belgian band formed in 1995 (it's been a while!), Hooverphonic. Listening this song makes me imagine a dramatic scene in an action movie, seriously. And the lyrics, I just love it.
BAC FM // Ça te dérouille les oreilles! – You'll probably notice some of the songs I love up there are French songs. How can I know those songs? I don't even live there. Actually last year, I found a huge links to European radio, and since J'ADORE FRANCE, I click on random pop/rock radio link and I found this radio. Now I listen to this radio almost every day during my work hour <3
Lastly to complete the list.. I present the best youtube band cover pour ce printemps *drumrolls* Le Comité Des Reprises! Originated from France (France alert again), formed by PV Nova and Waxx, and they are just so cool and talented. I mean, they change position on every covers. Sometimes Nova plays the guitar, sometimes he sings, plays the piano, etc. So does Waxx, he plays guitar, and then plays drums in the next cover, sometimes he plays bass. I just love this random thing in a band, changing position, lol. Here are two of my favourite covers which I also mentioned in previous best songs:
https://www.youtube.com/watch?v=Gzcla2CKud0 // Selah Sue "Alone"
https://www.youtube.com/watch?v=eDJRtzdTBhk // The Dø "Despair, Hangover & Ecstasy"
Let me know what do you think of this list and tell me what music do you listen to recently~