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Apicidin is a potent histone deacetylase inhibitor (HDACI) that selectively binds to histone deacetylases (HDACs) class I and interferes with the deacetylation process, which results in modification of acetylation level of cellular proteins. The aim of the study was to investigate the potential time and dose dependent cytotoxicity of the test compound, Apicidin, in pancreatic cancer cells Capan-1 and Panc-1 as well as estimate maximal tolerable dose (MTD) of the test agent and determine EC50 using four complementary colorimetric cytotoxicity or viability assays. The cells were treated with increasing concentrations of Apicidin (0-5000nM) for 2, 4 and 6h (short term exposure) or 24, 48 and 72h (long term exposure) before conducting cytotoxic analyses with lactate dehydrogenase assay or viability analyses with sulforhodamine B (SRB), methyl tetrazolium (MTT) and crystal violet (CV) assays. In order to investigate whether Apicidin irreversibly affects the cells already during the short term exposure, the medium containing Apicidin was removed and replaced with fresh culturing medium after 6h of treatment. The cells were then incubated for additional 24, 48 or 72h before carrying out the analysis. The results obtained from cytotoxicity and viability assays indicated, that Apicidin was well tolerated by both cell lines at concentrations below 100nM at any given time point and at all applied concentrations during the short term (6h or less) treatment. Continuous prolonged term exposures (48h or greater) of the cells to Apicidin with concentration exceeding 100nM resulted in significantly increasing cytotoxicity and sustained significant loss of cell viability. Moreover, long term exposure of pancreatic cancer cells Capan-1 and Panc-1 to Apicidin concentrations exceeding 100nM showed an initial anti-proliferative effect before cytotoxicity onset. In summary, MTD was exposure time dependent and estimated to 100nM for long term treatment and to at least 5000nM for treatment not greater than 6h. EC50 concentration of Apicidin was established after long term treatment, however with some variation when comparing the different assays and cell lines. Results from this study may encourage reinvestigating the capacity of potent HDACI Apicidin as an attractive agent for interfering with the deacetylation process catalyzed by HDACs for potential pancreatic cancer intervention.
Coelomocytes are macrophage-like cells in the body cavity or the coelomic spaces of many invertebrates and play major roles in their physiology and immunology. Their structure, function and diversity, however, is still poorly understood.
Tardigrades are micrometazoans inhabiting a wide variety of environments and with an ability to survive extreme conditions. Coelomocytes ("storage cells") represent an important part of tardigrade physiology, storing and distributing energy and possibly also having immunological functions. Few studies of tardigrade cell biology have been reported and neither primary nor continuous cell cultures have been established. Tardigrades are normally found and also cultured in an environment rich in microorganisms, some of which may even be of symbiotic value.
In this study we have tried to establish a primary culture of storage cells in the eutardigrade Richtersius coronifer. Different cell media and concentrations of fetal bovine serum (FBS) were tested. Extracting cells from the tardigrades in an antiseptical environment is challenging since it has to be done under a microscope and contamination from the tardigrades surface is also a problem. To avoid this we tried culturing with high concentrations of antibiotics and antimycotics. We managed to keep the cells viable for up to 18 days in Grace insect medium with 10 % FBS at 20-22°C. The medium was changed every third day. 10x Antibiotic-Antimycotic and 5x of Penicillin-Streptomycin were used to minimize contamination. These concentrations reduce the bacterial abundance, but contamination with fungi was still an issue. Cell morphology evaluation was performed daily and no obvious toxic effects on the cells was observed. Cell viability and cell division were evaluated with Trypan blue staining and cell counting in a haemocytometer. The results indicate that the cells are viable and that some cell division occurs, however more studies need to be performed to confirm this. Still, this study provides the first evidence that primary cultures of storage cells from tardigrades are possible to establish, but the culturing method has to be refined to avoid contamination.
We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for 1-3days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing etoposide-treated with untreated cells. The cell volume of the treated cell lines was initially increased at early time-points. By time, cells decreased in volume, especially when treated with high doses of etoposide. In conclusion, we have shown that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death. Future applications could include real-time monitoring of these holographic microscopy parameters in cells in response to clinically relevant compounds.
α1-Antitrypsin (AAT) is a major circulating and tissues inhibitor of serine proteinases implicated in the regulation of inflammation and host defence. There is now increasing evidence that AAT may also exhibit anti-inflammatory activities independent of its protease inhibitor function. This study was undertaken to investigate the effects of native (inhibitory) and polymerized (non-inhibitory) forms of AAT on MID (Moraxella IgD binding protein)-induced human tonsillar B cell activation in vitro. We found that 0.5 μg/ml MID induces B cell proliferation and stimulates IL-6 release (p < 0.001) relative to non-stimulated controls. Both native and polymerized AAT (0.5 mg/ml) inhibited MID-stimulated B cell proliferation in a similar manner (by 70%, p < 0.001), whereas MID-induced IL-6 release was more strongly suppressed by polymerized (9.9-fold, p < 0.001) as compared to native AAT (2.8-fold, p < 0.01). Electrophoretic analysis of cell culture media did not indicate any interaction between AAT and MID, and flow cytometry data showed no competition for the same receptor. The effects of AATs were observed whether added together with MID or 2 h after MID-addition to cell cultures. Thus, our data demonstrate that AAT inhibits MID-induced B cell activation in vitro that is unrelated to its protease inhibitory activity and is not dependent on MID binding to the cell surface.
Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from ∼8.1 to ∼7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus, V. tubiashii, V. splendidus, V. alginolyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by ∼65–90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5–3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens.
BACKGROUND/AIM: Androgen deprivation therapy is usually in the initial phase a successful treatment for prostate cancer but eventually most patients develop androgen-independent metastatic disease. This study investigated if manganese (Mn) reduces viability of prostate cancer via induction of apoptosis.
MATERIALS AND METHODS: The prostate cancer cell lines PC3, DU145 and LNCaP underwent dose- and time-dependent screening of viability, analyzed by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Flow cytometry was used for the cell-cycle and apoptosis analyses. Intracellular Mn concentration was measured using inductively coupled plasma-mass spectrometry.
RESULTS: At Mn concentrations of 200-1000 μM, the effect on viability was most pronounced in PC3 followed by LNCaP cells. These cell lines also showed higher intracellular concentration of Mn compared to DU145. In all cell lines, Mn increased the proportion of cells arrested in the G0/G1 phase and induced apoptosis.
CONCLUSION: To our knowledge, this is the first report demonstrating Mn as a potential agent in prostate cancer therapy.
Manganese (Mn) is an essential trace metal for all organisms. However, in excess it causes toxic effects but the impact on aquatic environments has so far been highly overlooked. Manganese is abundant both in costal and deep sea sediments and becomes bioavailable (Mn2+) during redox conditions. This is an increasing phenomenon due to eutrophication-induced hypoxia and aggravated through the ongoing climate change. Intracellular accumulation of Mn2+ causes oxidative stress and activates evolutionary conserved pathways inducing apoptosis and cell cycle arrest. Here, studies are compiled on how excess of dissolved Mn suppresses the immune system of various aquatic organisms by adversely affecting both renewal of immunocytes and their functionality, such as phagocytosis and activation of pro-phenoloxidase. These impairments decrease the animal's bacteriostatic capacity, indicating higher susceptibility to infections. Increased distribution of pathogens, which is believed to accompany climate change, requires preserved immune sentinel functions and Mn can be crucial for the outcome of host-pathogen interactions.
Forskningsmiljön "Man and Biosphere Health" är engruppering där forskare från helt olika biologiskakunskapsområden träffas och knyter kontakter. Forskning inom området "Life Science" (Livsvetenskap), som framförallt innefattar biologi, medicin och biokemi, är världens största tvärdisciplinära forskningsområde med studier av biologisktliv samt de förutsättningar som utgör grunden för fortsatt liv. Unikt för samarbetet inom MABH är kombinationen avekologisk och biomedicinsk kompetens, vilket i vårt fall har inneburit att cellbiologisk forskning har knutits ihop med miljöforskning på ett nyskapande sätt.
The invertebrate phylum Tardigrada has received much attention for containing species adapted to the most challenging environmental conditions where an ability to survive complete desiccation or freezing in a cryptobiotic state is necessary for persistence. Although research on tardigrades has a long history, the last decade has seen a dramatic increase in molecular biological ("omics") studies, most of them with the aim to reveal the biochemical mechanisms behind desiccation tolerance of tardigrades. Several other aspects of tardigrade cell biology have been studied, and we review some of them, including karyology, embryology, the role of storage cells, and the question of whether tardigrades are eutelic animals. We also review some of the theories about how anhydrobiotic organisms are able to maintain cell integrity under dry conditions, and our current knowledge on the role of vitrification and DNA protection and repair. Many aspects of tardigrade stress tolerance have relevance for human medicine, and the first transfers of tardigrade stress genes to human cells have now appeared. We expect this field to develop rapidly in the coming years, as more genomic information becomes available. However, many basic cell biological aspects remain to be investigated, such as immunology, cell cycle kinetics, cell metabolism, and culturing of tardigrade cells. Such development will be necessary to allow tardigrades to move from a nonmodel organism position to a true model organism with interesting associations with the current models C. elegans and D. melanogaster.
Adult Diffuse Large B Cell Lymphoma (DLBCL) is a heterogeneous form of hematopoietic cancer and difficult to treat. In order to find a better diagnostic indication for the disease, we analyzed Low Molecular Weight Protein Tyrosine Phosphatase (LMWPTP) that in humans is encoded by the ACP1 gene. LMWPTP is an enzyme shown to counteract Protein Tyrosine Kinases (PTK) and was suggested to be a negative growth factor regulator. However, the 18 kDa PTP can also have a positive effect on cell growth and proliferation, indicating a controversial role in the tumorigenic process. LMWPTP exists in different isoforms which are electrophoretically, kinetically and immunologically distinct. We have studied two subgroups of DLBCL consisting of a Germinal Center B cell like (GCB) and a non-Germinal Center B cell like (non-GCB) group. The two subgroups have been defined by gene-expressing profiling and are associated with differential outcome. The expression levels of LMWPTP protein was compared and showed significant differences between the GCB and non- GCB subgroups (p=0.012). Interestingly, when the samples were divided into survivors and non-survivors, and thereafter analyzed for LMWPTP expression, the samples from patients with a higher survival rate showed increased staining intensity, whereas the samples from patients with lower intensity of LMWPTP did not survive the disease (p=0.001). In conclusion, we have shown that DLBCL patients with worse outcome express LMWPTP with a lower intensity, suggesting a tumor suppressor role for this form of the enzyme.
Src homology 2 domain-containing tyrosine phosphatase-2 (SHP-2) is an important regulator of cell signaling because of its ability to dephosphorylate receptors of growth factors as well as the cytokines and tyrosine-phosphorylated proteins associated with these receptors. In the current study, we used four different prostate cancer cell lines: PC3, DU145, LNCaP and LNCaP-IL6+. Tumor specimens from 122 patients with prostate cancer were analyzed using a tissue microarray. Our data demonstrate that all four prostate cancer cell lines express the SHP-2 protein. Additionally, low staining intensity and SHP-2 expression in the cytoplasm of cancer cells in prostate tumor specimens was inversely correlated with prostate volume (p = 0.041 and p = 0.042, respectively) whereas nuclear staining was positively correlated with extracapsular extension (p = 0.039). In our post-prostatectomy specimens, we found that patients with low SHP-2 expression had less favorable outcomes with respect to biochemical recurrence and clinical progression (p = 0.005 and p = 0.018, respectively). The loss of cytoplasmic SHP-2 expression is associated with increased growth and prostatic cancer progression.
The protein tyrosine kinase (PTK) receptors and cytosolic signaling proteins as well as the protein tyrosine phosphatases (PTPs) have important roles in regulation of growth of the benign and malignant prostate gland. Here, we studied expression of the protein tyrosine phosphatase SHP-1 in prostate cancer cell lines and in human prostatic tissues. SHP-1 is expressed at a high level in LNCaP prostate cancer cells compared with PC3 cells. Silencing of SHP-1 expression with siRNA in LNCaP cells led to an increased rate of proliferation, whereas overexpression of SHP-1 by means of transient and stable transfection in PC3 cells led to a decrease in proliferation. Corresponding changes were observed in cyclin D1 expression. We further demonstrate that LNCaP and PC3 cells respond differently to IL-6 stimulation. SHP-1 overexpression in PC3 cells reversed IL-6 stimulation of proliferation, whereas in SHP-1-silenced LNCaP cells, IL-6 inhibition of proliferation was not affected. In addition, IL-6 treatment led to higher levels of phosphorylated STAT3 in SHP-1-silenced LNCaP cells than in control cells. Next, SHP-1 expression in human prostate cancer was analyzed by immunohistochemical staining of tissue microarrays comprising tumor specimens from 100 prostate cancer patients. We found an inverse correlation between the tumor level of SHP-1 expression and time to biochemical recurrence and clinical progression among prostate cancer patients. In conclusion, our results suggest that a decreased level of SHP-1 expression in prostate cancer cells is associated with a high proliferation rate and an increased risk of recurrence or clinical progression after radical prostatectomy for localized prostate cancer.
The protein tyrosine kinase (PTK) receptors and cytosolic signalling proteins as well as the protein tyrosine phosphatases (PTP) have important roles in regulation of growth and function of the benign and malignant prostate gland. Here we studied the expression levels and functions of the protein tyrosine phosphatase SHP-1 in prostate cancer cell lines and in benign and malignant human prostatic tissues. We found that SHP-1 is expressed at a high level in LNCaP prostate cancer cells compared to PC-3 cells. Silencing of SHP-1 expression with siRNA in LNCaP cells led to an increased rate of proliferation as measured by thymidine incorporation, whereas in PC3 cells in which SHP1 was overexpressed by transient transfection proliferation rate was decreased. We also examined SHP-1 expression in prostate cancer by immunohistochemical staining of tissue microarrays comprising tumor specimens from 122 prostate cancer patients. We found an inverse correlation between SHP-1 staining intensity and the time to biochemical recurrence as measured by a rise in the serum level of prostate-specific antigen (PSA) in prostate cancer patients. In conclusion, our results suggest that a low level of SHP-1 expression in prostate cancer cells is associated with high proliferation rate and with an increased risk of biochemical recurrence after radical prostatectomy for localized prostate cancer.
BACKGROUND: Interleukin-6 (IL-6) is a multifunctional cytokine that has been implicated in the modulation of growth and progression of prostate cancer. Decreased expression of the tyrosine phosphatase SHP-1, involved in regulation of cytokine and tyrosine kinase receptor signaling, has been shown to be associated with less favorable outcome among prostate cancer patients.
METHODS: Parental LNCaP cells and an LNCaP-IL6+ subline, derived from parental LNCaP cells by continuous culture of the cells in the presence of recombinant IL-6 were used in the study. Expression of STAT3, pSTAT3, ERK, pERK, AKT, pAKT, PTEN, and SHP-1 was analyzed by immunohistochemistry, Western blots, cDNA microarray, quantitative PCRs, and reverse transcriptase PCRs. Proliferation and apoptosis of transfected cells were analyzed by caspase3/7 assay and flow cytometry.
RESULTS: Phosphorylation of ERK and STAT3 was increased in the LNCaP-IL6+ subline compared with LNCaP cells, whereas pAKT was decreased. Overexpression and inhibition experiments with SHP-1 siRNA showed that SHP-1 reduced proliferation and increased apoptosis in both cell lines. Microarray analysis revealed 80 up-regulated and 87 down-regulated SHP-1-related genes in the LNCaP-IL6+ cell line compared with LNCaP cells.
CONCLUSIONS: SHP-1 suppresses growth and increases apoptosis in both LNCaP and LNCaP-IL6+ cells, which suggests that SHP-1 could be a therapeutic target in prostate cancer, even when there is an IL-6-related growth advantage.


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Top Super Foods That Will Cure Your Leaky Gut Problems for Good
Despite the growing epidemic of leaky gut, this condition remains confusing to the majority of people. If you are one of those people experiencing symptoms of leaky gut, you probably know that a lot of common health conditions are a result of leaky gut. Hippocrates, the father of modern medicine was right when he said "All disease begins in the gut."
Medically speaking, leaky gut syndrome is also called "hyperpermeable intestines." Unless you are studying medicine or have a strong knowledge in this field, you might not be able to fully understand what it means.
It's high time to sit down and know more about this condition, so you will be able to recognize it in yourself, or even in your loved ones.
Understanding What Leaky Gut Really Means
Before you know what leaky gut means, it is important to understand how digestive system works.
The first mechanism of defense of your immune system is the intestinal lining. Tight junctions connect the outer layers of intestinal cells. Microvilli are found at the tip of these cells, and they are responsible for the absorption of nutrients and transporting them into the bloodstream.
When the digestion process is normal, everything that we ingested is screened effectively since the tight junctions stay closed. What passes through the bloodstream are only the nutrients that are needed by the body.
To fully understand this process, picture a long line of excited party goers going into an exclusive bar. Due to the popularity of this bar, everyone should be screened carefully by the bouncers. They are tasked to make sure that only the elite party goers can enter, while ordinary people are rejected easily.
Those bouncers act like tight junction to filter the party-goers that can come in.
For whatever reason, the bouncers decided to leave their post and went home. Naturally, everyone grabbed this opportunity and went rushing into the bar. The supposedly exclusive bar is now filled with different people from all walks of life.
Having a leaky gut is like having those bouncers decided to go AWOL.
When foreign particles enter your body, it treats them as enemy, so they work so hard to try to get rid of them. Your body is on a war-mode to eject these foreign particles. In return, this could destroy the natural process of your body parts which could lead to inflammation and other variety of diseases.
The Usual Suspects: Know the Symptoms of Leaky Gut
Since leaky gut is often the root cause of many health problems, it is important to have this diagnosed and treated early. Below are the common symptoms of leaky gut.
- Allergies: Allergies are commonly triggered by particles like dust, pet hair, insects, molds and pollen. Did you know that it is also one of the symptoms of leaky gut in adults? Leaky gut may not be the first thing in your mind when you are uncontrollably sneezing and have runny eyes, but these two systems intertwine. Both digestive and respiratory tract's main job is to protect the body from foreign invaders.
- Asthma: With leaky gut, yeast, pathogens and poorly digested food particles can now pass through and may wander to other parts of the body such as sinuses and the lungs. They can cause inflammation which can result to difficulty breathing, chronic stuffy nose and wheezing.
- Autism: Nine out of 10 individuals with autism are also suffering from leaky gut. While there is no actual study yet that could explain the correlation of the two, but a hypothesis that because of leaky gut, foreign substances can pass into the bloodstream that harm the brain.
- Autoimmune Disease: Autoimmune diseases, in which the immune system is making antibodies to fight against its own tissues, are always associated with leaky gut syndrome. Treatment of these diseases includes healing of the gut.
- Eczema: When the gut is healthy, it contains about 80 to 85 percent of good bacteria and 15 to 20 percent of bad bacteria. When the particles are not filtered properly due to leaky gas, this ratio becomes out of balance. This can result to many skin diseases like eczema.
- Psoriasis: The connection between leaky gut and psoriasis is evident according to Steinmetz Center for Integrative Medicine. When there is a leak of protein from the gut, the body considers them as foreign substance. It then attacks these foreign substances by triggering an inflammatory, autoimmune response in the form of psoriasis.
- Inflammatory Bowel Disease: Once there is a gap in the intestinal linings, toxins can flow into the blood stream. Inflammatory bowel diseases (IBD) like ulcerative colitis and Crohn's are another symptom of leaky gut.
- Rheumatoid Arthritis: Rheumatoid Arthritis is one of the symptoms of leaky gut in men wherein the body's immune system mistakenly attacks the joints. When this happens, you may suffer from fatigue, severe joint swelling and cramps
- Systematic Inflammatory Response Syndrome (SIRS): When bacteria and other foreign organisms pass through the body due to leaky gut, your body's immune system automatically fights them off which could lead to inflammation. It's the body's response to these infectious particles. SIRS is an inflammatory state as a response of our body's immune system to infection.
- Type 1 Diabetes: This type of diabetes is a kind of auto-immune disease in which the pancreas is no longer producing insulin. Due to the inflammation caused by leaky gut, the immune system attacks the gland that is responsible for making insulin.
- Bloating: People with leaky gut often complain of gastrointestinal issues like bloating that are often accompanied by gas.
- Candida Overgrowth: Candida is yeast that can be found in the gut, mouth and birth canal. When there is an imbalanced immune system, Candida overgrowth is more likely to happen. Patients complain of experiencing skin and nail fungal infection, digestive issues and experiencing chronic fatigue.
- Constipation: People that are straining to have bowel movements and have hard or lumpy stool should blame their leaky gut as the culprit of this misery. Their digestive system is no longer functioning properly which contributes to the difficulty on passing out their feces.
- Ongoing Diarrhea: Chronic diarrhea can be a symptom of many disorders, one of which is the leaky gut syndrome. The toxins and bacteria that entered the body can be causing the diarrhea of people that has this syndrome.
- Gas: It is the stomach's job to break down foods and send them to the small intestines. When the stomach failed to do its job and passes large, unbroken particles to the small intestines, gas and bloating happen.
- Cramps and Pain: When you experience stomach pain and cramps after eating, blame the leaky gut as the culprit. Patients with leaky gut are also experiencing Irritable bowel syndrome (IBS).
- Joint Pain: Painful fingers, aching knees and chronic back pain are just some of the indications of leaky gut. The increase in inflammation in your body results to joint pain.
- Rash: Systematic and local inflammations which are caused by leaky gut contribute to a lot of skin diseases, including rashes. Having a good skin comes from the inside out so it is important to have a healthy gut.
- Migraines: The gut is the body's second brain and they are connected through extensive networks of hormones, neurons and chemicals that affect our moods. If you are constantly suffering from recurring migraine and get a reaction to most of the food that you are eating, it is highly likely that you have a leaky gut.
- Chronic Fatigue – Especially After Eating: Many people that are suffering from leaky gut syndrome often experience a condition where they feel debilitating fatigue after a normal extension that can't be alleviated by rest. A study suggests that to treat chronic fatigue syndrome and other chronic illness and major depression, leaky gut should be treated first.
- Food Sensitivities: Most of the people that have leaky gut syndrome are also having food sensitivities and allergies, particularly to wheat, dairy and other grains. There is a circular correlation between leaky gut and food sensitivities. This means that food allergies can trigger a leaky gut and a leaky gut can also cause food allergies.
- Reflux: When there is a leaky valve in the stomach, acid enters the esophagus. Because the esophageal valve that connects the stomach and esophagus can't properly shut, gastric juices end up sneaking up the pipe.
- Anxiety: Since the brain and gut are interconnected, not only the gut influences the brain but also the brain influences the overall health of the gut. If you have a leaky gut, pathogens and other harmful substance can enter the bloodstream and can reach the brain causing anxiety.
- Depression: Numerous studies are suggesting that leaky gut syndrome could be the root of chronic depression. Patients that have this syndrome are also showing a manifestation of depression.
- Insomnia: If you have not been getting a good sleep, blame the leaky gut. Leaky gut affects sleep since the pain associated with inflammation can make it difficult to sleep.
Now that you fully understood what leaky gut is and the symptoms of leaky guts in adults, is there anything you can do to alleviate this problem? Surely you do not want the quality of your life to be affected. The good news is that you can say goodbye to your leaky gut with your lifestyle and diet.
The Power of Food to Alleviate or Even Cure Leaky Gut Problems
Though there are a lot of causes that trigger leaky gut, one of the main culprits is food that you take in to your body. Gluten and other inflammatory foods like toxic and dairy foods are suspected causes of this syndrome. Reverse this cycle by healing the leaky gut with the superfoods that you can take in.
Below are the foods that need to be included in the patient's daily diet to get rid of leaky gut problems.
1. A Bowl of Bone Broth to Alleviate your Leaky Gut Problems
For most people, they associate the word "home" with a warm and delicious bowl of broth. They can still probably remember their mother bringing them with a warm bowl of broth every time they had fever or was sick. Broth, especially bone broth, does not only provide warmth and comfort, they also provide a lot of medical benefits.
How is bone broth prepared?
Homemade bone broth, which is one of the most powerful superfoods, is made by simmering the bones of an animal, preferably free-range or pasture-raised, for 24 to 48 hours in a slow-cooker. Sounds time-consuming but the long wait is definitely worth it.
This slow and low cooking process draws out the marrow, collagen and other healing elements from the bones, including minerals, amino acids, gelatin and glycine that help heal inflammation.
Why is bone broth good for the gut?
Gelatin that can be found in bone broth is beneficial for fighting food sensitivities, restoring strength of the gut lining, supporting healthy inflammation levels in the digestive tract and helping the growth of probiotics or the good bacteria in the gut.
Unlike many other foods that are difficult to fully break down, bone broth is soothing and easily digested. It's time to turn on the cooker and have your home filled with the delicious scent of a home-cooked broth.
2. Flaxseed Tea – Not Your Ordinary Tea
People that are health conscious have tried different type of teas that they could put their hands on – green tea, black tea, oolong tea, milk tea and white tea. They should also try adding flaxseed tea to their list.
What is a flaxseed tea and how is it made?
Flaxseed, which is also called linseed, is harvested from the flax plant, which has been used as a traditional medicine for hundreds of years. It is one of leaky gut natural remedies that you can easily integrate to your diet.
To make flaxseed tea, just simply pour 12 ounces of boiling water over a tablespoon of flaxseeds for 30 minutes to overnight. Drain the seeds out before drinking it.
Along with its many health benefits, flaxseed tea provides healing support to people with leaky gut problems due to its content of soluble fiber and omega 3-fats which have lubricating effect and are anti-inflammatory.
3. Switch to Red Cabbage to Say Goodbye to Leaky Gut Syndrome
Many fans of salad could not live without cabbage. Though green cabbage is quite popular and commonly used, it's time to put the red cabbage in the limelight. So what's the difference between these two aside from the color? While they are both good for you, red cabbage has more powerful nutritional profile.
Red cabbage which is also known as a purple cabbage is a type of cruciferous vegetable that is eaten either cooked or raw. Do not underestimate the power of this vegetable as it provides the following benefits such as:
- Boosts the immune system.
- Fights inflammation and arthritis.
- Reduces the risk of osteoporosis and aids healthy bones.
- Combats chronic disease.
- Promotes a healthy gut.
These benefits provide ailment to leaky gut syndrome and the symptoms associated with it.
4. Coconut Oil to the Rescue for Leaky Gut Problems
Amongst the super foods that cure leaky gut, coconut oil is something that no one should be without. What's in the coconut oil that makes it essential for those that have issues with their gut?
Coconut oil contains healthy saturated fats, which are important in healing your gut. It also contains capric, lauric and caprylic acids that have antifungal, antimicrobial and antiviral properties. If you want your gastrointestinal system to be cleansed, coconut oil used should be extra virgin, organic and cold pressed.
If you may be asking how to use coconut oil into your diet, the answer is quiet simple. There are a lot of ways to mix coconut oil into your daily food. Instead of using the regular oil in frying eggs, French toast, omelets and pancakes, use coconut oil instead.
You can add it to fruit and vegetables smoothies, sautéed peppers, pan-fried steaks and potatoes and even caramelized onions.
5. The Combination of Garlic and Ginger is a Sure Way to Say Hello to a Healthy Gut
Garlic and ginger are nature's gifts to people that are suffering from leaky gut syndrome. Let's put each of this food in the limelight and be awe of how powerful they are in treating this syndrome.
Take the case of garlic. All cooked meal becomes more delicious if they are added with a little more garlic. You can add as much as you want with no negative implications. Aside from its magical power in turning ordinary foods into a five-star restaurant worthy dish, it is also rich in natural oxidants. What do these natural oxidants do? They are known to provide healing qualities.
Due to this healing quality, garlic balances the stomach bacteria which is necessary in having a healthy gut. In addition, it removes yeast overgrowth. Two cloves of fresh garlic every day will work wonders.
Ginger, on the other hand, has its own healing qualities. It helps fight inflammation in the intestinal track caused by leaky gut. In addition, it does not have any negative side effects, so it is effective in reducing irritation. People that have leaky gut should carry a small piece of garlic that they can chew on every day.
6. Tighten Up Your Gut with Turmeric
Those who love curry must also feel the same strong emotion to its main spice, turmeric. There is no doubt that this powder is enjoyed by so many, aside from the fact that it gives a special kick to curry and other dishes, it is also one of the most powerful herbs.
The list of the benefits that turmeric offers is long, but one of them is to cure leaky gut problems. Turmeric is a powerful astringent that contracts the proteins in the bowel lining. This results in reduced gut permeability, since the spaces between cells are squeezed. In addition, it is antimicrobial and anti-inflammatory.
What are some ways to add turmeric to your recipes?
Have a cup of turmeric tea, turmeric eggs for breakfast and curried carrot soup. There are also turmeric supplements that you can take.
7. Improve Your Digestive Health with Aloe Vera
This plant is commonly known to soothe burns, while many people use it as a powerful ingredient in beauty products. While this popular knowledge is true, there's more to this wonderful plant.
It contains minerals, key vitamins, amino acids and enzymes that are vital for our bodies to work properly. People that have problems with their digestive system should embrace the benefits that aloe vera can give to them.
Aloe vera can help heal the lining of a damaged intestinal tract and encourages good gut bacteria. Although there are a lot of ready-made aloe vera products, you can make an aloe vera juice for yourself.
Here's how you can enjoy aloe vera's benefits:
- Cut open an aloe leaf and expose the clear gel.
- Using a spoon, scrape the gel out.
- Add one or two tablespoons to coconut water, fresh juice or water.
8. Use Supplements for an Optimum Gut Health
With the advancement of science and medicines, a lot of drugs and supplements have been created to aid you in having good overall health. Though relying on natural remedies to treat leaky gut, it would be an insult to our great scientist if we turn our back to what they have worked hard for.
Before choosing the brand to support your specific needs, do your own homework first. Supplementing with a high quality product for your gut health is absolutely necessary. Digestive Science Intensive Colon Cleanse is one supplement that people with leaky gut needs to be considered. It's a complete package that cleanses the colon and aids in an efficient digestive system.
Now you know that you don't have to suffer from the symptoms of leaky gut anymore. With the aid of these super foods, it's time to fill your kitchen with these essentials. You can now live a healthy and happy life as you say goodbye to your gut problems.
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subset: commoncrawl


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Printing, Commercial Printing, Packaging
About Us
r-pac is a leading global supplier of packaging and trims providing retailers and brands with creative and innovative solutions. From design and structural packing development, printed paper products, woven, printed and heat transfer labels, EAS to RFID item tagging solutions. r-pac lead the world in developing and maintaining brand equity supported by the" identify with us!" company ethos. Today the r-pac footprint stretches over 30 countries across North America, Europe, Asia Pacific, Latin America, the Middle East and Africa. From these global operations we provide solutions to a multitude of business sectors including apparels, electronics, cosmetics, toys, hardgoods and sports brands. Since our foundations were laid over 20 years ago, we continue to value imagination, initiative and a collaborative spirit to delivering growth. Powered by our performance driven team members, we constantly work to promote a different experience to our diverse client.
Business Category: Printing & Packaging


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MBIX is happy to announce another local ISP has connected to the peering fabric. Zirro (AS395588) is a startup ISP in Winnipeg. They've connected with two 10 G links, paired using LACP to make a logical 20 Gbps connection to the 167 Lombard switch.
Great to see that new networks recognize the value in connecting to MBIX. Keeping local traffic local improves performance, adds redundancy, and helps build the Canadian internet.


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Fish Oil is hot on the news lately. Fish oil is rich in DHA and EPA, two powerful fats responsible for things like: increased metabolic rate, improved fat burning, and reducing our risk for a host of diseases (cardiovascular disease, cancer, and diabetes). Also studies are showing that supplementing babies and children with DHA improves brain development and can result in smarter children. In fact, the supplementing begins during pregnancy.
According to Dr. John Berardi:
" The North American diet is unbalanced in the omega 3 and omega 6 ratio, being higher in the omega 6 and increasing our risk for disease. The current North American diet provides a ratio that is around 16 to 20:1 (omega-6:omega-3). This is likely due to the shift in dietary staples, which generally do not include foods like flax, hemp, walnuts, perilla, green leafy veggies, chia, fish and algae."
Its typically recommended using about 6-12g of total fish oil per day or 3-6g of combined DHA+EPA. The primary way most people are told to supplement are through fish oil, which is actually cod liver oil. However, there are some issues with this form of supplementation, such as traces of contaminants that are found in our fish supply. But where do fish get their DHA from? The algae they eat of course. Many experts recommend taking the form of these fats straight from the algae see below. This is also an option for vegans.
I went into my fridge and found my own bottles of fish oil and DHA supplements to compare. Here is what I found:
1.) Nordic childrens DHA-Cod liver oil-serving size1/4 tsp
618mg omega 3, 205mg EPA, 300mg DHA, 300mg Omega 9
2.) Nordic Baby DHA drops-1-4ml
1008mg omega 3, 328mg EPA, 500mg DHA, 480mg Omega 9
3.) DEVA algae DHA drops-100mg DHA per 7 drops for children
4.) Spring Valley Prenatal DHA-1 softgel, 200mg DHA
Below is some information about algae based DHA.
The bottom line, in my opinion, is that both sources of DHA/EPA are good. The best thing I would do is rotate the source and increase your consumption of the fats directly from foods. Getting it from different sources ensures that your hitting all your bases. If you are vegan, then you would need to use only the algae based. I will warn you, its got a strong flavor so you may want to just take the pills and avoid the drops!


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Started out as a franchise of Face of Men, Mordecai Skin still mainly targets male youth, who faces embarrassing facial problems. Yes, they're actually a men's facial parlour, however, also welcomes ladies. In fact, as according to their management, they do have a substantial number of female patrons, who placed themselves as 'guinea pigs' for their partners.
And frankly speaking, after my session, I am indeed contemplating to drag T over for one as well. If only he's not so against facial. T.T
Now what's that about? We'll get to it in a while.
If you didn't know, Mordecai was the cousin and guardian of Esther, Queen of King Xerves, who taught her the meaning of both inner and outer beauty. And by adopting his name, Mordecai Skin shows their belief that beauty runs deeper beyond the surface of our skin and their ideal in treating their customers' skin issue from inside out.
Using only Eve Taylor's products, which do not contain Lancolin, Mineral Oil Artificial fragrance or SC alcohol, and are natural and gentle to the skin,
But that's actually not the focus. See my face, no signs of redness or swelling at all! Pictures were also taken under normal lighting and I've not done any sort of filtering to make my skin look any better than how it looks on the mirror.
Even though facial IPL, other than to rejuvenate the skin, also helps to gradually shrink our open pores, I was obviously unable to see any visible changes in my pores size after this one session. However, my skin definitely felt softer and smoother, and the effect was pretty lasting. I was truly amazed at how smooth my skin felt when I was washing my face in the showers when I got home, and the soft and smooth feeling actually lasted even up till now (been 1 week!).
And you know what, Mordecai Skin only charges $130 for the entire IPL treatment session. The management actually very humbly admitted that they're not exactly very cheap when it comes to their treatment prices, even though they've tried their best to give customers the best price possible. But after checking with a friend, who has been doing facial IPL treatment at a home salon, I realised that prices at home salons can go up to $110 per session!
With just $20 more, we get much more convenience of not needing to head to a stranger's home alone, better services, and promises of NO hardselling.
Why not?
Mordecai Skin is located at
Blk 728 AMK Ave 6 #01-4214 Singapore 560728
For more information, visit their website HERE
Till then,


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Camera not working mac yosemite
Camera not working mac yosemite If the camera doesn't respond in Yosemite, try to restart your mac. In case, you are working with OpenCV, following errors might occur: QTKit didn't find any attached Video Input Devices! Warning, camera failed to properly initialize! Cleaned up camera. Program ended with exit code: 255 Solution for these errors is the… Read More »


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Chamfer Edging
Price: £2.40 Each (inc Vat)
We offer many different types of edging products; including brick edging, rope edging, chamfer edging, and much more. The items in our edging collection will finish off the design of your garden in style, adding a unique identity to your outdoor space. We offer incredible value, without compromising on quality – ensuring that your garden design is both durable and attractive.
Edgings are perfect for keeping the shape of your garden immaculate, and our range is ideal for patios, driveways, paths, flower beds, and lawns.
If you're looking for high quality edging for your garden, Minster Paving can provide you with exactly what you need. Together with our stunning paving collection, you can create a beautiful outdoor space to enjoy all year round.:


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Why are seniors are filing more and more bankruptcies?
November 2, 2010 Leave a comment
As reported on Fox Business, according to something called "Consumer Bankruptcy Projects", senior citizens (55 to 64 years old) are filing more bankruptcies now than they did in 1991. In 1991 they filed only 6.1% of all bankruptcies. By 2007 that number had risen to 15.3% and by 2009 it was up to 16.9%.
The numbers for the 45-54 year olds increased from 15.8% to 23.5% during the same period. The numbers declined for the 35 to 44 year olds and for the 18 to 24 year olds the percentages declined even further. The younger people seem to be filing a smaller and smaller percentage of the bankruptcies and the older folks are taking up the slack. My question is- are the younger getting more responsible and the older folks less responsible?
According to the study the reason for this is mainly credit cards. The older people are getting more credit card offers and they are using them. Older folks are apparently more likely to have 5 or more cards which is a dramatic increase over the last 16 years. Apparently seniors are less likely to negotiate with their credit cards for lower interest rates or payoff amounts. They seem to accept and thus get stuck with whatever the credit card company gives them.
The other factor that the study points out is that seniors (55 to 64 years old) are also likely to be paying for their children and their parents simultaneously. People are living longer and this age group often has living parents who need care. Their medical care can be expensive and seniors often turn to their credit cards and lines of credit to pay the medical debts of their parents.
Seniors may also have adult children who need help with their families so they turn to their parents who turn to credit cards and lines of credit. Sometimes these children get their parents to sign onto business loans to start businesses which ultimately fail. The parents then become responsible for the debt.
I have noticed a just such a change with my clients. I am seeing more and more seniors with credit card debt who are in need of a bankruptcy. They often have significant credit card and personal loan debt. They are often very ashamed that they have such debt but according to this study it is indeed very common.
The study predicts that as the baby boomers age the numbers of seniors who file bankruptcy will increase. Having the financial responsibility of care for both parents and children can really be expensive for this age group. It is not surprising then why they get into debt and need a bankruptcy.
I am hoping that people can at last not be ashamed that they must file for bankruptcy. I am convinced that it is a financial decision that should carry no shame.
I am a bankruptcy attorney practicing bankruptcy law in San Diego. Please visit my website for further help at www.farquharlaw.com.


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Information Technology
If you're looking for a career on the cutting edge of the digital frontier, then IT may be just what you're looking for. Information Technologists are in demand across Canada and around the world for their skills in computer maintenance, networking, Security and movement of information and Internet development. As more and more companies realize the savings a well planned computer infrastructure can produce for them, the more your skills as an IT specialist will be in demand. Train today and start your career in this dynamic, leading-edge field as an Information Technologist. All Academy of Learning College programs include preparation for Industry Certifications like; A+, Network+, Security+, MCSA, MCSE, MCP, MCITP and more. Adding these certifications to your list of credentials is a must have. The Kingston campus is a Prometric and Pearson Vue authorized test centre, so, as a student you can write you industry exams right on campus!
Diploma Programs
- Computer Service Technician (NOC 2242)
- Network Administrator (NOC 2281)
- PC Support Specialist (NOC 2282)


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How to Think Like a Financial Adviser
Overwhelmed by amorphous long-term money goals? Personal-finance consultant Ashley Feinstein leads a crash course to help you get a handle on your dreams.
Name (and Date) Your Goals
Says Feinstein: "Many people don't know how much it would take to reach their goals. So start by making each one specific and measurable—and attach a deadline. For example, 'I want to buy a house within five years.' Then fill in the blanks. Look at housing prices in your ideal area and work backward from the down payment. This gives you a number—an amount you need to save monthly to fulfill your five-year plan. If the monthly savings goal doesn't seem doable, you need to look at the variables. Do you really need a house that size? Is the neighborhood critical? And adjust for reality. You may need to add a year and subtract a bedroom."
Examine Spending
"I have clients track their expenses for at least a week prior to our first meeting and then for three weeks after. People hate this. But ultimately they say that learning where their money goes is a huge relief. You have to really look at your behavior to change it. To make your tracking easier, use a tool like Mint or MoneyWiz."
Tailor and Target Savings
"Most of us are saving for a few things at once—retirement, college, emergencies. Some savings accounts allow you to create 'goal buckets,' which make it easier to automatically put money toward each priority. Saving for emergencies is tricky because it's so vague. I have clients think through emergency situations to make it more concrete. The obvious one is loss of a job. How much money would you need per month to feel comfortable, keeping in mind that emergency spending is not the same as regular spending? (You'll eliminate frills like dining out, for example.) The rule you may have heard to set aside three month's salary is not necessarily right for everyone."
Do a "Values" Exercise
"Ask yourself what really matters to you. Understanding what you most want out of life helps you create financial goals you're motivated to stick with—and follow through on what it takes to reach them. Typically people find that they're overspending in areas that aren't even important to them. Let's say you discover that you value traveling and time with friends most highly. That's where your money should go. Maybe you're paying for convenience (ordering lunch at work and eating alone at your desk) when you would be happier long-term if you brought a bag lunch and put the money you saved toward a trip with pals."
Assert Yourself
"Get in the habit of asking for what you want. Instead of telling friends, 'I don't care where we go for dinner,' offer an idea that makes financial sense for you. People often worry, needlessly, that they'll be seen as lame for making smart choices. Get over that! You can practice assertiveness in other arenas—at work and when dealing with merchants. I was shocked by a price my dry cleaner gave me and told him so. He wound up giving me a deal. It's good practice!"
Personalize Your Approach to Debt
"The issue of whether to prioritize saving or paying down debt is an individual one. Killing high-interest debt as soon as possible of course makes sense. But for some people, erasing the smallest debts first helps build momentum. And there's an emotional component to certain debt. I had a client who was determined to pay off her parents before dealing with any other debt. In any case, reward your successes. Some of my clients throw 'debt-free' parties. Some keep charts and cross off debts as they pay them. "You might find it easier to reach goals when you get the people close to you involved. If you and a friend tackle debts and share goals and accomplishments, it can be like having a workout partner to keep you motivated and accountable."
For more from Ashley Feinstein, visit knowingyourworth.com.


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Big Dave's Word From cheese prices to new year strategies, Big Dave has you covered at pizzatoday.com/big-dave-ostrander
Pizza Tweets
Pizza jerks
Calzone with up to 2 toppings for only $5 today until 4pm! X-mas is next week, be a pal and give a Pizza Jerks Gift Certificate!
Why it works: This Tweet was posted right before Christmas when money is tight for a lot of customers. Five bucks is a great motivator, but chips and a drink will bring the check total up. We love the idea of gift certificates, and this Tweet puts the idea of lunch and a quick gift into customers' heads.
Stone Hearth Pizza
Office holiday party? Pizza, pasta, organic salads? Yep, we got that. Give @Stone_Hearth a call for your catering needs-- big and small.
Why it works: There's always an office manager who doesn't quite get things done on time or a boss who decides to be generous at the last minute. This Tweet is for them –– the Scrooges and procrastinators who need a fast idea for a company luncheon. And Stone Hearth fits the bill perfectly. This Tweet only needed a phone number for maximum impact.
PizzaToday, Big Dave Ostrander and some of the most successful independent pizzeria operators in the country have joined forces to create the world's first School of Pizzeria Management. Visit schoolofpizzeriamanagement.com
You can now "LIKE" School of Pizzeria Management on Facebook!
Facebook Pizza Feeds
Pizza Lucé Hopkins What's up Pizza Luce fanatics!?!? We got a $25 gift certificate lookin to go to a good home! All you gotta do is tell us what is your favorite thing about the snow. We will pick the winner at 2pm today so be sure to check back this post to see if you're a lucky winner!
Why it works: This Facebook post does a good job generating word of mouth for Pizza Lucé. It makes an instant fan of the winner, encourages Facebook followers to check the page during the day and brings in business. Once you get a customer in, you can upsell drinks and appetizers beyond the $25 gift certificate.
Cicero's Pizzeria, Mission Viejo, California Cicero's fans, we have something special for you! On 12/12/12 we will be offering two different pizza specials.
On Wednesday, you can enjoy two 12" cheese pizzas for $12 or one large 14" pizza with up to 3 toppings for -- wait for it-- $12!
These special one-day deals exclude specialty pizzas and are for take-out only, and they cannot be combined with any other offers or coupons. Each additional topping for either special will be $1.25. We hope you're hungry because we'll be ready to go. Share this post and bring some friends! Hope to see you all on 12/12/12!
Why it works: The 12/12/12 buzz offered businesses some easy marketing fodder and Cicero's took advantage. The promotion provisions were posted right there, and it encouraged guests to share it on their own wall. Best yet –– the one-day promotion was easy to implement.
| Keep up with the latest trends, profit making ideas, delicious recipes and more. Delivered hot
and fresh to your email every Wednesday.


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1. What should I wear?
Please wear something comfortable that gives you room to move. No need to get dressed up! If you know you will dance hard and sweat, bring along a towel too!
2. Do I need special shoes?
No! We often dance bare foot because we want to feel our toes and feet and often we are in a yoga studio which requires no shows anyway. If you have need to wear a shoe please be sure it is a shoe that does not have dirt or mud on the bottom – preferably a shoe worn only indoors.
3. I don't know how to dance and have never had lessons. Is this for me?
Yes yes! This is not about performance or technique! We move our body – we follow very simple facilitation. Maybe we might be asked to soften through the knees and pulse gently, or feel the music in our fingers and gradually allow it to extend up into our arms. We might expand and contract our body with our breath, or take the rhythm into our feet. We might be invited to join in on a circle dance, or we might circle and encompass a participant who is requesting support and healing. If you enjoy dancing, if you love to move to rhythms and would really like the opportunity to move freely with people who share that interest then this is the right place for you!
4. I am self conscious about dancing and moving in front of other people. How do I handle this?
Don't worry – you are not alone! Most of us feel a bit uncomfortable at first. Some of our most dedicated participants were tremendously nervous and self conscious when they started – you should see them now! As often as we can, we darken the room and use candlelight and we often begin with closed eyes. This is not about competition – or looking slick. It's about joining with understanding and friendly like minded people in a very safe space!
5. What kind of music is used?
The music is not rock and roll. It is not classical. It is unique lush compilation of world beat rhythms , women's voices, funk, minimal techno, spiritual, ambient and selections that foster a desire to begin moving. Often the music will evoke an emotional or creative physical response allowing the participants to delve into various aspects of their being. Emmy has researched many hours to find just the right music to accommodate this practice. Often people will request a CD of the music which is not allowed because of legal restrictions – but I will share the artist so you can find the music yourself if you wish to do so. Sometimes we dance to live drumming!
6. Can I just come and observe?
No. That could be very intimidating to the participants. Those of us who are participating do not enjoy being watched by someone. Often people get intensely involved and need the freedom to laugh, cry, explode, go crazy, or just chill to the beat. Just as you would probably not be comfortable having someone sitting and observing you doing your moves – we all feel the same.
7. May I bring my child?
Generally we invite ages 12 and up to join. Women sometimes bring their younger daughters and they are welcome to come, as long as they are able to participate and not be a distraction. You know your own child. We only request that you not bring a child who does not wish to participate. Please be aware that sometimes at the closing circle – it is a period of quiet time and if your child is unable to handle that we just request that you take the child out of the room at that time. Very often young children enter into the experience fully and enjoy.
8. Is Dance On the Wild side a religious practice?
No – but it is often a spiritual experience. Dance On the Wild Side honors all Religions. Anyone can share in this without compromising their own religious belief. Most likely the experience will enhance and support whatever is your religious experience.
9. Do men come and participate?
Sometimes events do include men . The Friday night DOWS at Studio 30 C Essex includes men and some of the big Conscious Dance events also welcome men.


text: "I love my new farm! Trish was diligent, proficient, informative, and moved the buying process along quickly and with just the right amount of genuine enthusiasm. Trish possesses the skills necessary to create a fine real estate experience, either on the buying or selling side, and I highly recommend her services to others."


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Apple Watch App-Chirp for Twitter
Missing Twitter on Your Apple Watch? Well thanks to Will Bishop, the high school senior from Australia, you can enjoy your Twitter feed because he's created the perfect app! Podcast Interview Check Will out in the App Store. Also, listen to his podcast interview with the guys on Apple WatchCast Podcast. It's interview number 272 on March 11th. You can listen to the interview on Apple's podcast app or Overcast, as well as other podcast apps you can find in the App Store. Thanks for stopping by today!👩🏻💻 Continue reading Apple Watch App-Chirp for Twitter


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Have you been on prescription medication or drugs for many years and your life is sometimes spinning out of control? At Exclusive Ibogaine SA we use pioneer medicine that has been registered as a Schedule (6) medicine in SA from 1 June 2016.
Ibogaine is only legal in a few countries in the world ie Canada, Netherlands, New Zealand, Mexico, Gabon and some European states. It originated in Gabon, West Africa and has been used in Western society since the 70's with remarkable life changing results. Ibogaine is a psychoactive plant that is acknowledged across the globe as an effective treatment to combat Substance Dependency, Drug addiction and provide a better life quality for those suffering from Stress, Depression, Anxiety, Post Traumatic Stress Disorder, Chronic Pain, Compulsive Behaviour and various other mental and physical disorders.
Ibogaine works on the Neuro-Transmitters in the brain, not only does it brake physical dependencies to all kinds of drugs and stimulants but it also restores all the homeostasis of the body, serotonin and dopamine levels, cholesterol, blood pressure, sleeping disorders which ultimately play an integral part in a healthy body. On an emotional level this remarkable plant medicine bring powerful insights on personal and emotional issues, it resets the brain and restores life energy, realign those negative and self defeating emotions and acts like a defrag of traumatic experiences.
This 36 hour journey has also been described as a life time of therapy with the best psychologists - our sub-conscious minds. During this time a life overview might come to the mind's eye like in an awaking dream state. In an objective way traumatic life experiences might be revisited and a sense of peace, letting go and life changing introspection and insight will replace the devastating memories.
Our Clinic provides a Five Day Ibogaine-Assisted Detoxification Therapy and Treatment Program under medical supervision and with the attendance of our qualified personnel
Our Program Director, Anita Viviers is the clinic's Founder and General Manager and has more than 4 years experience as an Ibogaine Treatment Facilitator with over 500 successful treatments under her personal supervision. A success rate of 60-70% has been documented in breaking substance dependency and treating mental disorders. Together with our Stress Consultant who help overcome trauma, anger, depression, grief and loss, fears and phobias, anxiety, panic attacks, addictions, relationship issues etc. with 11 years experience.
Exclusive Ibogaine SA follows the guidelines provided by The Global Ibogaine Therapy Alliance (GITA) and in accordance to the Manual for Ibogaine Therapy: Screening, Safety, Monitoring & Aftercare - Second Revision by Howard S. Lotsof & Boaz Wachtel and are modelled on the best practices of an ever-evolving body of knowledge.
Pre-admission procedures
The first step is to complete our Application form supplied by our office.
You are requested to visit your GP for a Stress ECG, Liver and Kidney Function Test & Pregnancy Test – if female and submit them via e-mail, these tests need to be approved by our Medical Practitioner at least 48 hours prior to arrival.
The Five Day Ibogaine Program gets individually designed and consists of:
- A full flood dose of Ibogaine according to our dosing guidelines with compliance to the Ibogaine Alliance.
- Psychological and Life Coaching sessions with our accredited counsellors.
- A uniquely designed nutritional recovery program.
- Reintegration Recovery Program consisting of workshops and practices such as Vision Board & Life Purpose Workshop, Stress Management, Yoga, Meditation, Reflexology
- Educational group sessions and various social-integration activities.
What makes Exclusive Ibogaine SA Clinic unique is providing a safe and professional treatment environment with our experienced and well-trained staff. We provide individually designed recovery programs for our patients. A specially tailored Re-integration Recovery Program that equips our patients with the skills necessary to deal with life challenges in a constructive manner for relapse prevention.


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City of Joliet, ILHome Menu
Garbage, Recycling and Yard Waste
Waste Management will resume bulk Item pickup for residents that pay for municipal waste on their water and sewer bills on regularly scheduled garbage and recycling pickup days starting Monday, May 11, 2020. Bulk items are defined as furniture, mattresses, carpet and appliances.
Residents are asked to call (815) 280-7854 to notify Waste Management when placing a bulk item out for pick-up at their location. Additionally, residents are asked to limit their bulk items to 1 per week until the volume returns to normal in a few weeks. This is strictly to ensure that WM collection teams can complete all the core services each week.
For garbage, recycling and yard waste questions, contact Waste Management Customer Service Center at 815-280-7854 OR live chat with them at www.wm.com (scroll towards the bottom and click on CHAT and enter your information). Chat is available from 7 a.m. to 5 p.m. Monday through Friday only. Customer Service Center is available 7 a.m. to 6 p.m., Monday through Friday, excluding holidays, and from 8 a.m. to 12 p.m. on Saturdays.
Residential recycling and garbage is picked up weekly. Yard waste pickup began on March 9, 2020 and will end on December 11, 2020. Yard waste is picked up weekly on the same day as garbage and recycling.
Please remember to always have your garbage, recycling and yard waste out at the curb by 7 a.m. on your pickup day.
Electronics and household hazardous wastes can be disposed can be disposed of for free through Waste Management's At Your Door Special Collection program picks up electronic wastes and household hazardous wastes from your home for free if you pay for municipal waste on your water and sewer bill. Call 1-800-449-7587 or go to the website www.wmatyourdoor.com to schedule a pickup. More information can be found by going to the electronics wastes and household hazardous wastes information pages. See links on the left of this page._______________________________________________________________________
Not sure what day garbage, recycling and yard waste are picked up at your address. Click here for a map of the day (Monday-Friday) garbage is picked up in your area.
For more detailed information about the City of Joliet 's Garbage, Recycling and Yard Waste Program - Download a flyer in English or in Spanish.
The City of Joliet has contracted with Waste Management, Inc., to provide collection service for garbage, recyclables, and landscape waste (during season) throughout the City of Joliet on 5 different days of the week. On certain holidays, the collection service does not occur on the scheduled day, but collection service resumes on the following day. The Holiday Collection Schedule for 2020 is listed below. If you have any questions concerning the schedule or your pick-up day, please contact Waste Management at 815-280-7854.
Remodeling? Waste Management can provide 10, 15, 20, or 30 cubic yard containers for your home improvement project. Call Waste Management customer service department at 815-280-7854 for a quote. Additionally, Waste Management has provided 100 bagsters to the City of Joliet for its residents for free. One bagster per household until they run out are available at 150 W. Jefferson St (City Hall), Joliet, IL 60432. Bagsters normally cost approximately $30 at local hardware stores and they are 3 cubic yards. This is ideal for small construction projects and cleanouts. Citizens will still be required to pay the cost of pickup, which is approximately $176 (prices subject to change). Citizens would need to call 1-877-789-2247 to arrange for the bagster to be picked up. If you were to get a dumpster this size it would cost approximately $300 (prices subject to change).
Medications (unused, expired or unwanted)
Medication should NEVER be discarded down the drain or thrown into the garbage. Unused, expired and unwanted medication is a health and safety risk, as well as a potential threat to waterways. Drop off locations for medications are located at several locations in Joliet and in Will County.
Residents should research to make sure the medication(s) you are trying to discard qualify for drop off at any of the locations prior to dropping off. For locations and more information, please click here.
Moving? Garbage and Recycling carts are the property of Waste Management. Please leave the garbage and recycling carts for the next resident. Your cooperation is appreciated.
Holiday Schedule: Waste Management only observes the following holidays. When your pickup falls on or after the holidays listed below, your pickup will be one day late during the week.


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Yes, readers, it is indeed that time of year again! Football Manager 2011 was recently released, and after becoming completely addicted for what will probably be the better part of the next few months, I've decided it's about time I just got the Football Manager 2011 review written up and get on with my game as soon as I can. So here goes…
The transition to Football Manager 2010 from 2009 took quite a bit of getting used to. We saw an overhauled user interface which was received with mixed reception, although after over 200 hours of gameplay, I got used to it in the end.
We were promised a number of improvements for FM 2011, but not much mention was given of the interface this time around. This is because the user interface remains mainly the same, with not many changes on that front. There is, however, a slightly revamped theme as default which I really dislike. There is a dark skin on its way though, so no need for panic there.
When you're preparing your team for a match, you will be able to take advantage of the new training system that has been put in place for the new version. The core training feature remains familiar, but you have additional options when heading in to the next game. You can now set a focus area for your players heading in to a fixture, so they can concentrate on what you feel needs more work before the big game.
There is always a risk when playing games such as Football Manager that they can go too far, too in depth, too complicated. It goes without saying that you want to effectively manage your team, but you don't want to get to the point where you need to spend an hour before every match on preparing tactics to prevent a heavy defeat.
The way the additions to the training system in FM 2011 are implemented strikes a great balance, and gets it just right so that both casual gamers and committed managers can enjoy the experience.
You can now negotiate with potential players in real time, although you will have to get past another, new obstacle in the form of agents (and we all know what they can get up to).
Contract negotiations by nature are meant to be frustrating, and with the new system in FM 2011, Sports Interactive has managed to remove some frustrations with the game's system, and add frustrations from real life. As far as I'm concerned, it makes the whole experience a lot more realistic, and that's never a bad thing when you're dealing with a simulation game.
To improve this aspect of the game, Sports Interactive has included over 100 new animations, as well as more player emotions, pitch textures, improved lighting, floodlit night matches and more goal celebrations. Win win situation, don't you think?
Overall, Football Mananger 2011 is a steady upgrade from FM 2010, with lots of refinements making for a really pleasant experience – unless you find yourself at the bottom of the league fighting for survival. If you want to check it out for yourself, you can grab it for just under £25 from Amazon, or £29.99 from Steam.
Hoping to study Computer Science at University in the near future, you'll seldom see John without a computer in touching distance! His interests include building computers, reading all sorts of literature and of course writing for Zath to keep you updated on all the latest in the world of tech! You can follow John on Twitter as @british_geek.


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As a mother of four children, I often need to escape my reality. I need to find another place in time. I have come to love opening a book, and losing myself in the characters, and letting their lives seem real enough for me to understand their feelings. I have walked dusty English roads for miles in a dress alongside Jane Austen's characters; I have crawled inside a hobbit house and traveled to far away imaginary lands thanks to J.R.R. Tolkien; and I have enjoyed numerous young adult novels that are easy to read, and are usually not complex enough to add any stress to my tired brain.
I had heard mixed reviews of The Time Traveler's Wife by Audrey Niffenegger. The story sounded intriguing to me, even though one friend of mine said that she had walked out of the theater within the first fifteen minutes of the movie. Maybe that made me even more curious. I bought the book. The author's detailed descriptions of the smells, the temperature, the out-of-sequence times and dates, and the unexpected age changes of the characters drew me in almost immediately. She described how Claire and Henry met, and explained that Henry traveled through time. I got lost in the complexity of the novel, and forget about my daily tasks. I found myself thinking of the sweet characters continually.
In one part of her novel, Audrey Niffegegger describes Henry's condition as a neurological disorder. She explains that stress, alcohol, and lack of sleep can bring on a sudden episode where Henry travels in time. This bit of information grabbed at my heartstrings, and struck a chord so close to home. I could feel his anguish and his pain. His episodes of time travel came on just as my daughter's episodes of seizures begin. Stress and lack of sleep are triggers, but sometimes the brain just acts up with no obvious triggers. The brain is complicated, and it is often difficult to regulate abnormalities.
Henry and Claire couldn't do much to control Henry's neurological disorder. They learned how to cope and how to live with his condition. As whimsical as the idea of time travel is, this story has stayed with me, and given me much to think about in the years since I have read the novel. While I was trying to escape to a fictional place, the reality of my life kept seeping in; and now the fictional tale still seeps into my reality.
Bella has gone for two or more years with no seizures a few different times in these last 20 years. We live life as normally as we can every day. During the long periods with no seizures, we let our guard down, but we still know that at any given moment, she can have a seizure. That seizure will undoubtedly turn into a cluster of seizures. Our lives may be interrupted for a couple of days, weeks, or months. For us, the clock sort of stops ticking. Things that are going on in "the real world" are simply no longer that important to us. Days and nights in the hospital run together. Other people may be celebrating birthdays or holidays or the first days of a new season, but we are in another place in time.
Sometimes I see it as our own little way of traveling in time. We share little moments that we don't always get in the normal hustle and bustle of a busy school day or work day. We stop and smell the roses. We appreciate the little things, and we pray for things to return to "normal." But, like Henry, we don't really have full control of our time.
Although Bella's epilepsy is horrible, and has added undue stress to our lives; although it has stolen moments in time that we would have liked to have been able to spend differently; we are grateful. Because of these lapses in time, our family has been able to let go of reality from time to time, and we have grown closer. We are far from perfect. We don't always get along; but we can handle a crisis. We are stronger because of it.
Life with a neurological disorder is not something I would wish on anyone, but these little occasions of stepping into another place in time are in some ways a blessing. I am thankful I read a book that put this idea in my head so that I can relax, and accept the times in our lives that we do not have control of everything.


text: USS 2016 Users' Conference - November 9th & 10th 27. June 2016 Admin (0) Make plans to attend our 2016 Annual Users' Conference scheduled for Nov. 9th and 10th at The Inn at Opryland in Nashville, Tennessee. Registration form and agenda to follow soon! Room reservations are now available at:Book your group rate for United Systems Nashville Meeting


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Key Characteristics
With a few exceptions, students must be enrolled in Title I to attend the program, and parents must register the children. Children who are not enrolled in Title I may attend on the basis of teacher recommendations and low scores on standardized tests. The program focuses on enrichment activities, rather than remediation for students who have failed a class.
Because of the emphasis on individualized attention, the number of children accepted into the program each year determines the number of teachers hired. Most classes have about 15 students.
Hands-on activities are key to student learning; each class takes at least three field trips and students perform experiments, read books, write stories, and solve mathematical problems. For example, first-grade classes follow the life cycle of the frog by raising tadpoles in their classrooms. Students keep records of their observations; measure and graph tadpole growth; and write stories, riddles, and fact books about their frogs. At most schools, teachers use videos, films, and audiocassettes to enhance classroom instruction. In addition to teaching staff, each site has a media specialist who serves as a librarian and conducts classes that focus on reading activities (e.g., acting out stories, creating new illustrations for a book, reading to children, having children read to her).
Overall management is a team effort; the site directors meet once a week with the district coordinator, and the site director and teachers at each site meet at least once a week to discuss program issues. In addition, the staff receives occasional input from an oversight task force composed of the director of specially funded programs, the coordinators of alternative learning and summer enhancement programs, several principals, and a community representative.
Logistical issues are not a problem, according to a site director and the program coordinator. Most of the children in inner-city schools walk to the program; students who live more than three miles from the school ride on school buses provided by Title I. The sites have not had problems obtaining air conditioning, security, or other facility needs. At most sites, parents must make a commitment not to let family vacation plans conflict with a child's participation in the program.


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Some time ago, I saw a TV program about how focusing our concentration on something can affect our brainwaves. I was most fascinated by a demonstration by a marksman. As he initially pointed his gun at the target, his brainwaves showed little change. But as he took careful aim, tuning out all distractions around him, and focusing intently on the target, his brainwave patterns were radically altered. This test revealed the precise moment when the marksman pulled the trigger, because it indicated the increased intensity of his focus and concentration, just before he fired his weapon.
This demonstration reminded me of how we can "take aim" with our prayers the same way. While it's true that we can pray anywhere, anytime, it's also true that there are times when we need to separate ourselves from the distractions of the world, so that we can focus intently on our communication with God, aiming our prayers with precision. This is one of the reasons why there is simply no substitute for spending time alone with God. Jesus demonstrated this principle perfectly. The Bible records numerous times when the Savior "went off to a solitary place, where He prayed." (Mark 1:35 NIV) If the Son of God needed to spend regular time alone with His Heavenly Father, how much more do we need to? Jesus was a busy Man, and He had important work to accomplish. The verses above in Luke 5 tell us that crowds of people with pressing needs were constantly demanding His attention. Even so, we're told that "Jesus often withdrew to lonely places and prayed." The Savior knew how important it was for Him to regularly withdraw from the distractions and pressures of the world around Him, and to focus His attention solely on God.
Just before He went to the cross, when His anxiety and anguish were at their peak, Jesus withdrew to the Garden of Gethsemane to pour out His heart to God. As Christians, we have the same privilege. Jesus told His disciples: "When you pray, go away by yourself, shut the door behind you, and pray to your Father secretly. Then your Father, who knows all secrets, will reward you." (Matthew 6:6 NLT) When we make the effort to get alone with God, and to prayerfully focus our attention solely on Him, He will reward us with untold blessings that we may not receive any other way. One of these blessings is divine revelation. Mark 4:33-34 (NIV) says: "With many similar parables, Jesus spoke the word to them, as much as they could understand. But when He was alone with His disciples, He explained everything." This passage not only emphasizes how the Lord uses His Word to speak to us, but it also reveals how He takes special care to explain things more fully to those who spend time alone with Him.
It's been said that "God speaks to those who listen." God wants to speak to every one of His children, but He doesn't want to compete with the radio, TV, or anything else that steals our attention. The devil has seen to it that our lifestyle is embracing more and more noise. And the sad part is that we've become so accustomed to the noise and distractions around us, that we've almost forgotten how pleasant and soothing peace and quiet can be. Several years ago, I began challenging myself to limit the amount of noise and distractions I come in contact with each day. I began to leave the radio off when I was driving my car, and I used this time to pray and fellowship with the Lord. I also began doing household chores in complete silence, using the time to meditate on Scripture, and to sing praises to God. Making these little changes in my life helped me to become more attuned to God's "still, small voice," and to grow closer to Him.
Hearing an encouraging word from a friend, or in a sermon, can really make a difference in our lives. But nothing can impact us the way that hearing a direct word from God can. The Lord is able to speak words of wisdom and comfort like no one else can, and He longs to do exactly that. But we must do our part by making an effort to separate ourselves from the hustle and bustle of our daily lives, and to sit quietly in His presence. When we do, the Lord will not only speak to our hearts, but He will also refresh us, and give us rest. The Bible tells us about a time when Jesus and His disciples were so busy that they didn't even have a chance to eat. The Savior told them, "Come with Me by yourselves to a quiet place and get some rest." (Mark 6:31-32 NIV) The Lord extends this same invitation to you and to me every day. May we always choose to accept it, so that we may experience God's awesome presence, power, and peace in life-changing ways!
Prayer: Lord, forgive me for the times I've failed to give You first place in my life. Do a new work in my heart, so that I will do whatever it takes to get alone with You for a while each day. Teach me how to tune out the noises and distractions around me, so that I can tune in to Your voice and aim my prayers with precision. Thank You, Lord, for leading me into a deeper and more rewarding relationship with You!
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British Airways and Air France-KLM rise. Lufthansa seeing more reductions in premium traffic
Shares in British Airways, Lufthansa and Air France-KLM advanced 2.0%, 1.6% and 0.6%, respectively yesterday (25-Jun-09), despite overall weakness in European stock markets.
Investors shrugged off Lufthansa's commentary that while Economy class demand has been stabilising over the past few months, premium travel demand continues to decline at a rapid pace. The carrier confirmed plans to remove a further seven aircraft (for a total of 25 aircraft) from service in the remainder of 2009, with plans to remove five aircraft from its European fleet next month and two long-haul routes in Sep-2009.
British Airways, meanwhile, stated 6,940 staff (or approximately 17% of the carrier's workforce) have volunteered for unpaid leave (4,000 staff), part time work (1,400) or unpaid work for a period of one to four weeks (800) as at 24-Jun-09, potentially saving the carrier as much as GBP10 million.
Europe selected airlines daily share price movements (% change): 25-Jun-09


text: While waiting for some friends at Hotel DeLuxe, I used my extra found time and Draplin's field notes to draw a picture. Of note were a crazy chandelier, a stuffy chair, and two dachshunds that arrived with a man who ordered a gin and tonic. His wife got a double.