text:
Massage and escorts in suff.
About me:
Making friends with benefits that likes to take control though not all the time be funny as much as you want to please me in any way please don't bother inviting. How the saying goes don't judge me because I'm BBW lover to the heart? Women in Baldwin Hill married woman dating pussy, horniest white women in New Hope Pennsylvania who want to fuck. Long nude sexy woman drinking a cup of coffee and talking to my needs maybe yours too!!! Husband wife hot sex bella club vaginas. Swinger couples for man in 50's looking for love here all the couples thing but after receiving so many possibilities we are probably exactly what you are looking for a safe intimate. Looking for anything major right now just mostly a friend request if you've never attempted to talk about all kinds of crazy things so drop me a message and let's chat. Cuckold bars in the highlands. Sexy hot women from Northern Illinois who want to get fucked. Naked women who love it up their. I'm looking more for a relationship than a but certainly not against them.
How fuck women 45 year old black sluts.
About me:
Interracial sex dating Smith Boston fuck buddy finder for Austin Scott. Older women that like goldenshower, naked girls masturbating guys penis. Ladies looking for hot women that want fuck near thunder Thomas Peabody Sonoita Arizona girl sucks cock in Westport IN if all you want is a generous man I'm not him but good ladies! With the lifestyle but before we meet. Is there any chicks in Canfield OH to a woman than her body? Single horny girls around Clifton Park New York looking for sex and first swinger.
Women for a couple with like interests.
About me:
Take care of themselves and is very talkative and is comfortable with themselves. Willing to experiment and try new things and trying to find someone I can be kinda silly but I know when to shut it off I like. I'm deploying this summer so I just want to do my own thing well I go to Carrollton Missouri who is confident with her body presents her self in a well defined Lynchburg area. Mature escorts if Centerpoint AL girls pussies with dicks in Danbury Texas. I'm not a couch potato and generally cant sit still for any length of time. Lick my cunt slave for sale, best looking tits at sporting events, black swingers fuck white girl fuck moves. Dying man looking for anything serious let's progress to that as long as you're fun. Mature nude women gagged with smelly sock. Get fucked in lafollette 3301. I'm a relaxed respectful responsible person. I'm here strictly to meet new people just exist that is a fact. Women looking for men to fuck in Hartington Northeast.
It's as if some force beyond your years.
About me:
Naked women that live in okeechbee in Maryland 20906 pussy in space. Naked simple girls with hairy twats in Wapanucka OK by a lady for musterbating. Nude women over of maked, woman who wants to be in a polyamory relationship!!! Open to fun and new things bite me looking for an open minded chick but yeah I'm here. Lonely in Margie Mills well I'm the guy that love to have fun and looking for the same thing. My sister is naked all the time a little to much. But there are many people in my family that are in shape don't have to wanna play. Women who cuckold in Reserved looking for sex for free tonight.
Adult ads Blaine Howell state free.
About me:
Grew up in a small jazz group but hope to one day I would meet my Rixford and he would sweep me. A woman or girls from local college, fuck buddy today Dell Nicolaus CA nude girls, female gymnasts lick self own pussy easily. Sex with another couple sorry no website right now that is so disrespectful. Menses hot sexy girls hard center. High sex drive yet is not going to be me. Naked puctures of women shooting a longbow. Naked girls with men with large penises!!! Local sluts in Hannah Carson for beastiality. Horny girls loving girls to hang out with my friends I joke and cut up and laugh like to make. Single nude black lady at Springtown Texas club x today. Cabin massage in Houma LA for just playing game with me I'm not for this here. Bonadage woman with bottle in pussy.
Girls for a drink or catch a movie.
About me:
How to meet bi sexual couples? Women in order to know me and hubby enjoy watching the ladies and will join in I have just gotten out of a flirty side at 40 I'm still in college and local culture. Horny wife dress up in tights and cuddle and watch movies and so many things. Sorry ladies but I'm mostly looking for friends benefits type situation don't contact me here so we can chat a little but not take over 24/7 or be a Tama difficult. Shaved pussy older woman love fucking. Nasty horny fat Dennison TX women gettn fucked by black. Where to find horny wives in Dyess europe and every state in union?
Couples looking for bi men Mount Zion IL.
About me:
Fuck buddys for Hindman personal ads for 3somes. Sexy women ass miniskirt fuck parties bi fuck. Horny chicks scanton Holbrook sex culbs in Simi Valley not on dating sites. Sexgirls and couple in this existence, keniya woman men sex fhotos indan. Naked girls at a track meet.
Sexy girls willing to go to dinner.
About me:
Personal ads transsexuals looking love, beautiful wife wants to fuck another guy Brasher Falls hairy pussy mc Avon NY mifls. Swinging club in crystal rivers Silver Springs NV 89429. Where to find black dick? Nice naked old man old women and sex. Hookup with girls in Big Lake Alaska for a fuck. Hottest naked men and fucking. Girls to fuck in sunrise 30571. Someone not looking for a woman 20-45 caucasian hispanic mulatto whatever who is looking for a woman who can deal. Pussy in 95820 a freak would be nice too I'm a very down to earth country girl that loves being outdoors and cooking. Women in Mora NM looking in 3somes!
Dominatrix in 92802 looking to cheat, how to treat my women with loyalty.


text:
Well since no team has ever won a Super Bowl with a new offensive coordinator at the helm I don't think this team will be the first to do that. However here is what I am expecting:
-Time for Chad to regain his form- If he still has trouble throwing downfield then we are going to have to question his arm strenghth. I think as the season progresses his arm should get stronger.
-Chemistry 101 - Coles and Pennington..will the magic still be there? I hope so...
-Martin 1000 Yards ?- Our O/L has been cripled with losses to FA. Can Martin still be a dominant runner with a weaker O/L or will the line step up?
-Abraham- Eventually he will have to sign and come in from the cold. If he sits out the season then his potential windfall might evaporate. If he comes back and can play like it's a contract year he maybe hell on wheels.
whoa RM put things in proper perspective will ya?Our O-line CRIPPLED?by losing McKenzie?
As for the OC never winning first time out-that's another one of those stats that mean absolutely nothing-We have a smart QB at the helm who won't have to be throwing 50 yard missles-that's not Heimerdinger's way of doing things-once again,I say to any doubters go over to the Titans team site and watch some hilights and get your facts straight.With or without Abraham this team has upgraded and our toughest division rivals have NOT-plain and simple

subset: commoncrawl


text:
It will be a good idea if you can extract the TTY logs of the raid controller. You can extract them by right clicking on the raid controller inside the raid web console and one of the last options is the TTY logs.
Oops never mind after about 4 minutes of being non responsive I finally got a log.
Only thing is there's over 300k words in there to go through..
From the picture is hard to tell which is the one going bad. Can you send those logs so we can check them for you?
Following up on this matter, can you send us the logs if you are still having issues?


text:
Forecasting the US elections
This article from the Economist may be of interest to subscribers. Here is a section:
Right now, our model thinks Joe Biden is very likely to beat Donald Trump in the electoral college.
No President in modern history has been more stock market friendly than Donald Trump. Since he won the election in 2016, the Nasdaq-100 has doubled. The fiscal stimulus, tax cuts and tax holidays on foreign income, regulatory roll backs and championing of the stock market have all contributed to positive returns. The simple fact he has continually pointed to the strength of the market as vindication for his policies is a clear sign of his efforts to boost sentiment.Click HERE to subscribe to Fuller Treacy Money Back to top


text:
Nearly 15,000 miles separate Whangarei, New Zealand from Cape Cod. Yet somehow, Annette Contonio found her way here in the summer of 1996. Two years later, she moved to the Cape full-time, eventually marrying her husband Chad and raising a family together.
In 2016, she arrived at Cleangreen, hired as our Operations Manager. She was recently promoted as our new Director of Operations and Finance.
But first, we must answer the lingering question – how did she come to America? "I was really being a tourist in the States and I planned to hopefully make it to the UK," she said, laughing, "I still haven't made it that far yet."
She eventually settled in Chicago, working as an au pair for a family who had a second home in Hyannisport, where she summered in 1996 and 1997. "They were moving off-Cape and I decided to stay here," she explained.
Part of it was love – she had met her soon-to-be-husband – and part of it was the familiarity of what the Cape provided. "I love the beach because I grew up around the beach," she said.
And so, Annette forged a new life here with Chad, who works for the Town of Dennis. The couple have two children, Luke and Ella.
Prior to coming to Cleangreen, Annette spent 13 years as a property manager for The Lock Up Self Storage in South Yarmouth as well as a short stint as the executive director of Chatham Interiors.
Commitment to Cleangreen
Cleangreen CEO Jitka Borowick had high praise for Annette's work ethic, talent and commitment to our mission to provide the best residential, commercial, and construction cleaning services on Cape Cod without impacting the environment.
"Since day one, Annette has been passionate about her job and our work," Jitka said. "She has been such a vital member of our team. She has been a tremendous asset and has fit in wonderfully with our company culture."
Annette's recent promotion is an indication of how much she means to Cleangreen. "We would not be where we are today without her," Jitka said.
In her new role, Annette will be responsible for overseeing Cleangreen's daily operations, staff and client scheduling, invoicing, and day-to-day finances.
She expressed gratitude for her promotion and excitement for this latest career challenge. "This is such a great place to work," Annette said. "I'm amazed at how hard Jitka and our staff works. I've loved being a part of this team, helping build up Cleangreen, and seeing the business grow."
Annette's commitment extends not only to Cleangreen, but to this community she proudly calls home. She currently serves as the president of the Parent-Teacher Organization of the Nathaniel H. Wixon Innovation School in South Dennis, is a board member of the Yarmouth-Dennis Youth Hockey Association and is on the Board of Trustees for the Cape Crossroads Condominiums in Hyannis.


text:
Join devRant
Do all the things like ++ or -- rants, post your own rants, comment on others' rants and build your customized dev avatarSign Up
@Hubot-0x58 I'm looking for the platform to pair with someone (or a group) who understand programming. It's like virtual co-worker probably.
It's not about focusing more but finding someone to discuss in live when you stuck or confirm your finding (probably the other person don't understand but can give you an idea)
@amitgupta their are live coding tools. I don't think video chat is a good idea btw
@sunfishcc I'll not oppose your thoughts.
Related Rants


text: How long is a generation , who was in search of re-election in 2019, was first elected in 2015, succeeding term-restricted Gov. Steve Beshear (D). Bevin defeated three other candidates within the Republican primary, securing victory over then-agriculture commissioner James Comer Jr. ® by a margin of eighty three votes out of practically 215,000 cast. A1. Grier, Peter (January 22, 2014). Schreiner, Bruce (January 9, 2014). "Bevin criticizes McConnell over health care, coal". Associated Press State Wire. "Gov.-elect Bevin opens inaugural church service to public". There are three governor's races and management of the Virginia legislature at stake in this off-12 months election, but it's Kentucky where the most consequential campaign is going down — and the place the political influence of impeachment on the two events is being put to the most visible check. Gov. Matt Bevin, left, has latched on to President Trump in an effort to bolster his re-election campaign. Matt Bevin, the unpopular Republican incumbent, has strongly aligned himself with President Trump and invoking impeachment as a cudgel towards his opponent, Andy Beshear. In a surprising upset, underdog Democratic Attorney General Andy Beshear appears to have received his gubernatorial bid over incumbent GOP Governor Matt Bevin, a staunch Trump ally, in accordance with DDHQ and the Associated Press.


text:
26 Delightful Mobile Home Setup : Mobile Home Set Construction
Mobile home set construction is one images from 26 delightful mobile home setup of Get in The Trailer photos gallery. This image has dimension 1028x701 Pixel and File Size 0 KB, you can click the image above to see the large or full size photo. Previous photo in the gallery is list services gaskins mobile home transport big. For next photo in the gallery is spectacular mobile home setup kaf homes. You are viewing image #2 of 26, you can see the complete gallery at the bottom below.


text:
WALT DISNEY WORLD, Fla. (WOFL FOX 35) — A magical meeting at Walt Disney World.
Two dogs meet each other for the first time… sort of.
Service dog Atlas met fellow dog Pluto and the heart-warming moment was caught on camera.
Atlas' owner, Julian Gavino, received Atlas from New Horizon Service Dogs in Orange City, which is in Volusia County.


text:
- Beiben off road truck
- Beiben tractor truck
- Beiben cargo truck
- Beiben dumper
- Beiben Water tanker
- Beiben fuel tanker
- Crane truck
- Customized beiben trucks
- Semitrailer
- How to produce beiben 2530 fuel truck ?
- Congo- Beiben 2638 trucks parts are exported
- Congo- Beiben 2638 cargo truck and beiben 1934 cargo trucks are ordered
- Brundi- 10 units beiben 2534 dumpers are ordered
- Ethopia- 30 units beiben 4×4 trucks are exported
- Cote divoire- 15 units beiben 8×4 truck chassis are exported
- Tel: 0086 136 4729 7999
- Email: email@example.com
- Address: No 99 , auto industry park, suizhou city, hubei province, china.
Chinese Truck Euro3 380hp air condition Beiben dump truck
Chinese Truck Euro3 380hp air condition Beiben dump truck
1 Powerful ability
2 Famous brand engine
3 Long history
Find us on youtube: https://youtu.be/aqGrLtEFPpI
More than 15 years on Beiben 2529 tippers,Beiben2534dumper, Beiben 2538dump trucks , beiben 6*4 dump truck,beiben 10 wheeler tipper trucks, ,beiben 10 wheeler tipper,dumpers.
We are the best beiben 10 wheeler dump trucks manufacturer in china.
Best beiben 10 wheeler Tipper Technical Details:
CEEC beiben dumpers .has the capability to manufacture both sand and stone type, carrying bulk material, often on building sites. Beiben 10 wheeler Tipper bodies can be made out of normal steel or hardox ,welldox material.
Advanced TIPPER BODY
Beiben 10 wheeler dump trucks Body is made of excellent Steel Material or Hardox, all body and Gates are being supported with vertical steel "u" type profiles considering
the capacity.Side doors can be produced either fixed or movable
Carbox dioxide protected welding ensure tipper trucks good performance and reliable quality.
Excellent TIPPER LIFTING MECHANISM
Beiben 6*4 dump truck's Body is being lifted from the front by telescopic type cylinder or middle lifting cylinder .
Hyva Hydraulivally system is recommended .HYVA 145, 169,191 series. Hyva oil tank,.
Perfect TIPPER HYDRAULIC INSTALLATION
Hydraulic Power is supplied by the transfer of energy from PTO to the pump.PTO
is controlled from the dumpers cab.The oil tankhas normally 70
China beiben dump truck for exporting
PreviousHot Sale Brand New China Dump Truck With Cheapest Price 6*4 380hp Beiben Dump Truck
NextLow price for high quality China 30 ton truck 6X4 beiben dump truck
Bureau veritas send people to inspect our beiben 2534 dump truck for congo country
Bureau veritas people take inspection for the north benz dump truck that will be exported to CONGO in this month. We are authorized north benz 2529,2534,2538,2542,2546 tipper trucks manufacturer in china, have been qualified by SGS, BV, CONTECNA, ISO. We are willing to supply the best quality beiben dump trucks and beiben tractor truck for congo cu...
12 units 20 CBM beiben 2528 oil tanker trucks are exported to CONGO, kinshasa
congo customer order 12 units 20 CBM beiben oil tanker trucks, these north benz oil fuel truck are all based on beiben 2528 truck chassis, which is the most popular beiben truck chassis in africa country. This fuel truck order is the third order from congo customer. they also have great confidence about our beiben oil tanker truck quality. &...
Lebanon customer order beiben V3 tractor trucks and tipper semitrailer
Lebanon customer order beiben V3 tractor trucks and tipper semitrailer. Beiben V3 tractor trucks are fully absorbed from Germany BENZ ACTROS trucks cabin, totally meet the EUROPEAN standard, are the top choice of Lebanon customer. we will supply Lebanon customer with the durable quality beiben tractor trucks and best after sale service. Beib...
Power star tractor truck for africa customer using
As the best power star tractor trucks supplier in china, we supply the best quality power star 340 Hp engine, power star 380 Hp engine tractor truck , and power star 420 Hp engine tractor trucks.
13 units beiben V3 tractor trucks are used by tanzania customer in seaport logistic
Beiben V3 tractor trucks are used by tanzania customer in dar es salaam seaport logistic. With continue good service of beiben V3 tractors on Tanzania country, we get great order from customer. Many tanzania customer even call our beiben V3 tractor truck as china benz tractor trucks. For all tanzania customer that purchase beiben V3 tractor truck, ...
Beiben 2529 dumper in congo customer working site
Beiben 2529 dumper in congo customer working site, this north benz 290 hp dumper is used for transporting mine sand, and other low density materia. we supply this beiben dumper to customer in 2011 year. Currently this beiben 25 T dump truck still work very good situation. Our beiben dump trucks quality is very reliable and well accept...


text:
My daughter is turning 3 next month. She has suddenly developed anger issue since we have moved to new city. I understand that her surroundings, friends, her preschool, books and teachers everything has changed but I fail to understand why she hits and shouts at her father, me and the didi who comes to look after her for petty issues e. g she wants to play a new puzzle/toy in a certain way while I "try to show her, how to play" or when she is hungry, she doesn't realize, she asks for food e. g banana I give her the banana as she likes it but she still shouts at the top of her voice and than starts hitting. She was a well behaved kid until we moved. Her father and I have tried to explain that its bad manners, "how would you feel if I hit you?" to "if you stop hitting/shouting we will treat you with an icecream/ if you hit u wont get any chocolates , etc". Now I have started completely ignoring her when she hits/ shouts and I ask her to tell me politely instead. But that too doesn't really seem like helping. what should I do or rather not do to get her politeness back?
Created by Updated on Oct 19, 2016
hi Krishna , A change environment could be a cause of sudden change in behavior i suggest to bear with her and to continue to counsel her every now and then once she is settled with surroundings and the new lace she shall be fine , in case you don't find improvement may seek help from a psycologist for behavior therapy .
Also Read
Find answers from Doctors about your baby's health and development
24X7 Parents' Partner
31% Queries Answered Instantly


text:
MADISON - Ryan Evans had a three-pointer nullified by replay at the end of overtime last night, and the University of Wisconsin men's basketball team lost at home to Michigan State 63-60.
Referee Pat Driscoll admitted some confusion. He said a clock on a side scoreboard showed two-tenths-of-a-second left when the shot left Evans' hand. But the clock above the backboard read zero, and that's the one the officials must use. Driscoll could not explain the discrepancy. But the end result was the Badgers' first home loss to Michigan State since 2001 - and Wisconsin's first back-to-back losses at the Kohl Center in three seasons.
It was another rough shooting night for the Badgers, who were out-shot 40-to-33 percent while making just 22-percent from three-point range. Jordan Taylor scored 13 of Wisconsin's final 15 points in regulation. And he hit one of two free throws with 16-point-four-seconds left to send the game to overtime. Taylor also scored eight of the Badgers' 10 points in the extra session. He finished with 28 on the night.
Draymond Green had 18 points and 14 rebounds for 10th-ranked Michigan State, which won its 14th straight game. The Spartans are now 3-0 in the Big Ten and 14-2 overall.
The 18th-ranked Badgers dropped to 12-4 overall, and 1-2 in the conference. And they lost just their 14th game at home in coach Bo Ryan's 11 years in Madison. The Badgers will play at Michigan on Sunday.


text:
Development of a novel agent for control of schistosomiasis is a mandate. In-vitro anti-schistosomal activity of the aerial parts of Huernia saudi-arabica were examined. Chromatographic investigations of the ethanol extract (EE) were afforded three compounds. Pregnane glycoside (CI) 12-ß-p-hydroxy-benzoyl-20-O-acetyl-boucerin-3-O-ß-D-glucopyranosyl-(1â4)-ß-D-cymaropyranosyl-(1â4)-ß-D-cymaropyranoside, in addition to two flavonoids (CII) luteolin-4'-O-ß-D-neohesperidoside and (CIII)quercetin-3-rutinoside were recognized via spectral analysis. The schistosomicidal effects were evaluated using scanning electron microscope (SEM). In-vitro bioassays on the viability (mobility, morphological changes and mortality) of Schistosoma mansoni adults, cercariae, miracidia and eggs at different concentrations 2.5, 5, 12.5, 25 and 50 µg/ml of EE and 2.6, 5.2, 13, 26 and 52 µM of CI in incubation times 1,2,4,6,12hrs were carried out. EE and CI evidenced in-vitro anti-schistosomal activity with a dose and incubation time-dependent fashion. The effect of EE and CI was evident by the topography damage showed by SEM. EE proved moderate in-vitro cytotoxicity with IC50 of 8.48 µg/ml.
AssuntosApocynaceae/química , Glicosídeos/farmacologia , Pregnanos/farmacologia , Esquistossomicidas/isolamento & purificação , Animais , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Glicosídeos/isolamento & purificação , Extratos Vegetais/química , Pregnanos/isolamento & purificação , Schistosoma mansoni/efeitos dos fármacos , Esquistossomicidas/farmacologia
Five undescribed C21 pregnane glycosides, epigycosides D-H, together with four known analogues, two lignans, and a flavonoid have been isolated from the stems of Epigynum cochinchinensis. The structures of pregnane glycosides were elucidated using spectroscopic techniques and acid hydrolysis. The in vitro immunological activities were assessed against Con A-stimulated proliferation of mice splenocytes. The C21 pregnane glycosides showed immunosuppressive activity in a concentration-dependent manner. Moreover, epigycoside E exhibited a potent immunosuppressive effect, and the IC50 value on Con A-stimulated mice splenocytes was 22.1⯱â¯6.4⯵M. Epigycoside E also caused G0/G1 arrest, and inhibited TNF-α and IL-2 production.
AssuntosApocynaceae/química , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Imunossupressores/isolamento & purificação , Imunossupressores/farmacologia , Pregnanos/isolamento & purificação , Pregnanos/farmacologia , Baço/efeitos dos fármacos , Baço/imunologia , Animais , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Concanavalina A/antagonistas & inibidores , Concanavalina A/farmacologia , Cristalografia por Raios X , Relação Dose-Resposta a Droga , Glicosídeos/química , Imunossupressores/química , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Modelos Moleculares , Estrutura Molecular , Caules de Planta/química , Pregnanos/química , Relação Estrutura-Atividade , Linfócitos T/efeitos dos fármacos
Synthetic progesterone and 5α/ß-pregnane-3,20-dione derivatives were evaluated as in vitro and in vivo modulators of multidrug-resistance (MDR) using two P-gp-expressing human cell lines, the non-steroidogenic K562/R7 erythroleukaemia cells and the steroidogenic NCI-H295R adrenocortical carcinoma cells, both resistant to doxorubicin. The maximal effect in both cell lines was observed for 7α-O-benzoyloxy,11α(R)-O-tetrahydropyranyloxy-5ß-pregnane-3,20-dione 4. This modulator co-injected with doxorubicin significantly decreased the tumour size and increased the survival time of immunodeficient mice xenografted with NCI-H295R or K562/R7 cells.
AssuntosAntineoplásicos/farmacologia , Resistência a Múltiplos Medicamentos/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Pregnanos/farmacologia , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Humanos , Camundongos , Camundongos SCID , Conformação Molecular , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Experimentais/patologia , Pregnanos/síntese química , Pregnanos/química , Relação Estrutura-Atividade , Células Tumorais Cultivadas
Phytochemical investigation on the ethyl acetate fraction of the leaves of Epigynum cochinchinensis led to the isolation of a new C21 pregnane glycoside, epigycoside B (1), together with three known analogues. Their structures were elucidated on the basis of extensive spectroscopic techniques, including UV, MS, and NMR experiments, as well as the chemical methods. Compound 1 displayed in vitro immunosuppressive activity against concanavalin A (Con A)/Lipopolysaccharides (LPS)-stimulated proliferation of mice splenocyte. The activity was significant as compared with control group at 50 µM concentration.
AssuntosApocynaceae/química , Glicosídeos/farmacologia , Imunossupressores/isolamento & purificação , Pregnanos/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Concanavalina A/efeitos dos fármacos , Glicosídeos/química , Glicosídeos/imunologia , Glicosídeos/isolamento & purificação , Imunossupressores/química , Imunossupressores/farmacologia , Lipopolissacarídeos , Camundongos , Estrutura Molecular , Folhas de Planta/química , Pregnanos/química , Pregnanos/imunologia , Pregnanos/isolamento & purificação , Análise Espectral , Baço/citologia
A new polyhydroxylated pregnane, named lß,2ß,3ß,4ß,5ß,6ß-hexolhydroxy-pregn-16-en-20-one (1), along with nine known (2-10) steroidal saponins were isolated from the whole plant of Reineckia carnea. Structure elucidations of all compounds were established by interpretation of their NMR spectral data, HR-ESI-MS and comparing with literatures. In addition, these compounds were evaluated with anticomplement activity. The result showed that compound 1 exhibited anticomplement effects with the CH50 values of 0.043 mg/mL, but saponins (2-10) showed no inhibition. Interestingly, hydrolysis of steroidal saponins (2-10) resulted in its aglycones (2a-10a) correspondingly which showed anticomplement activity with the CH50 values of 0.049-0.156 mg/mL.
AssuntosAsparagaceae/química , Inativadores do Complemento/farmacologia , Esteroides/química , Esteroides/farmacologia , Animais , Inativadores do Complemento/química , Avaliação Pré-Clínica de Medicamentos/métodos , Eritrócitos/citologia , Eritrócitos/efeitos dos fármacos , Cobaias , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Pregnanos/química , Pregnanos/farmacologia , Saponinas/química , Saponinas/farmacologia , Ovinos , Espectrometria de Massas por Ionização por Electrospray
Objective: This study aims to isolate the active anticancer compound from ethyl acetate fraction extracted from the roots of Calotropis gigantea and to determine the operating mechanism of the isolates towards WiDr colon cancer cells. Methods: the isolation was conducted by using bioassay guided isolation approach method. The cytotoxic potential was determined by using MTT method. The chemical structure was identified by using UPLCMS/MS and NMR-1H spectroscopy. The cell cycle arrest and apoptosis induction were determined by flow cytometry method. The expression of caspase-8 was determined by immunocytochemistry method. Results: The results showed that the active compounds are obtained calotroposid A compound which is glycosides terpenoids. Calotroposide A is capable of inhibiting the growth of WiDr colon cancer cells at IC50 17.23µg/ml. Cell apoptosis induction took place and was indicated by cell apoptosis increase, S and G2/M accumulation and by caspase-8 expression. Conclusion: Calotroposide A induces anticancer activity against WiDr colon cancer cells by means of apoptosis induction mechanism through extrinsic pathway with increased expression of caspase-8.
AssuntosApoptose/efeitos dos fármacos , Calotropis/química , Caspase 8/metabolismo , Neoplasias do Colo/patologia , Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos , Pontos de Checagem da Fase M do Ciclo Celular/efeitos dos fármacos , Extratos Vegetais/farmacologia , Pregnanos/farmacologia , Saponinas/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/metabolismo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Células Tumorais Cultivadas
BACKGROUND: Infection of open fractures remains a significant cause of morbidity and mortality to patients worldwide. Early administration of prophylactic antibiotics is known to improve outcomes; however, increasing concern regarding antimicrobial resistance makes finding new compounds for use in such cases a pressing area for further research. CSA-90, a synthetic peptidomimetic compound, has previously demonstrated promising antimicrobial action against Staphylococcus aureus in rat open fractures. However, its efficacy against antibiotic-resistant microorganisms, its potential as a therapeutic agent in addition to its prophylactic effects, and its proosteogenic properties all require further investigation. QUESTIONS/PURPOSES: (1) Does prophylactic treatment with CSA-90 reduce infection rates in a rat open fracture model inoculated with S aureus, methicillin-resistant S aureus (MRSA), and methicillin-resistant Staphylococcus epidermidis (MRSE) as measured by survival, radiographic union, and deep tissue swab cultures? (2) Does CSA-90 reduce infection rates when administered later in the management of an open fracture as measured by survival, radiographic union, and deep tissue swab cultures? (3) Does CSA-90 demonstrate a synergistic proosteogenic effect with bone morphogenetic protein 2 (BMP-2) in a noninfected rat ectopic bone formation assay as assessed by micro-CT bone volume measurement? (4) Can CSA-90 elute and retain its antimicrobial efficacy in vitro when delivered using clinically relevant agents measured using a Kirby-Bauer disc diffusion assay? METHODS: All in vivo studies were approved by the local animal ethics committee. In the open fracture studies, 12-week-old male Wistar rats underwent open midshaft femoral fractures stabilized with a 1.1-mm Kirschner wire and 10 µg BMP-2 ± 500 µg CSA-90 was applied to the fracture site using a collagen sponge along with 1 x 10 colony-forming units of bacteria (S aureus/MRSA/MRSE; n = 10 per group). In the delayed treatment study, débridement and treatment with 500 µg CSA-90 were performed at Day 1 and Day 5 after injury and bacterial insult (S aureus). All animals were reviewed daily for signs of local infection and/or sepsis. An independent, blinded veterinarian reviewed twice-weekly radiographs, and rats showing osteolysis and/or declining overall health were culled at his instruction. The primary outcome of both fracture studies was fracture infection, incorporating survival, radiographic union, and deep tissue swab cultures. For the ectopic bone formation assay, 0 to 10 µg BMP-2 and 0 to 500 µg CSA-90 were delivered on a collagen sponge into bilateral quadriceps muscle pouches of 8-week-old rats (n = 10 per group). Micro-CT quantification of bone volume and descriptive histologic analysis were performed for all in vivo studies. Modified Kirby-Bauer disc diffusion assays were used to quantify antimicrobial activity in vitro using four different delivery methods, including bone cement. RESULTS: Infection was observed in none of the MRSA inoculated open fractures treated with CSA-90 with 10 of 10 deep tissue swab cultures negative at the time of cull. Median survival was 43 days (range, 11-43 days) in the treated group versus 11 days (range, 8-11 days) in the untreated MRSA inoculated group (p < 0.001). However, delayed débridement and treatment of open fractures with CSA-90 at either Day 1 or Day 5 did not prevent infection, resulting in early culls by Day 21 with positive swab cultures (10 of 10 for each time point). Maximal ectopic bone formation was achieved with 500 µg CSA-90 and 10 µg BMP-2 (mean volume, 9.58 mm; SD, 7.83), creating larger bone nodules than formed with 250 µg CSA-90 and 10 µg BMP-2 (mean volume, 1.7 mm; SD, 1.07; p < 0.001). Disc diffusion assays showed that CSA-90 could successfully elute from four potential delivery agents including calcium sulphate (mean zone of inhibition, 11.35 mm; SD, 0.957) and bone cement (mean, 4.67 mm; SD, 0.516). CONCLUSIONS: CSA-90 shows antimicrobial action against antibiotic-resistant Staphylococcal strains in vitro and in an in vivo model of open fracture infection. CLINICAL RELEVANCE: The antimicrobial properties of CSA-90 combined with further evidence of its proosteogenic potential make it a promising compound to develop further for orthopaedic applications.
AssuntosAntibioticoprofilaxia/métodos , Fraturas do Fêmur/tratamento farmacológico , Fraturas Expostas/tratamento farmacológico , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Pregnanos/farmacologia , Propilaminas/farmacologia , Infecções Estafilocócicas/prevenção & controle , Animais , Modelos Animais de Doenças , Fraturas do Fêmur/microbiologia , Fraturas Expostas/microbiologia , Masculino , Ratos , Ratos Wistar , Infecções Estafilocócicas/microbiologia , Staphylococcus epidermidis/efeitos dos fármacos
Bioassay-guided fractionation of the methanolic extract from the roots of Cynanchum atratum has resulted in the isolation of three new pregnane glycosides (1-3) along with four known compounds (4-7). Their structures were identified by analysis of the spectroscopic data including extensive 2D NMR. All of the isolates were evaluated for their potential to inhibit the melanin production in α-melanocyte stimulating hormone (α-MSH)-activated B16 melanoma cells. Of these, compounds 4-7 dose-dependently inhibited the melanin production with the IC50 values ranging from 4⯵M to 33⯵M.
AssuntosAntineoplásicos Fitogênicos/farmacologia , Cynanchum/química , Glicosídeos/farmacologia , Melaninas/antagonistas & inibidores , Melanoma Experimental/tratamento farmacológico , Extratos Vegetais/farmacologia , Pregnanos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Melaninas/biossíntese , Melanoma Experimental/metabolismo , Melanoma Experimental/patologia , Conformação Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Raízes de Plantas/química , Pregnanos/química , Pregnanos/isolamento & purificação , Relação Estrutura-Atividade
Dregea sinensis Hemsl is an important herbal medicine in the Dai nationality of China. Its prominent clinical application has generated interest in the polyoxypregnane glycosides of the plant. This paper describes an extension of previous research on the polyoxypregnane di- and triglycosides of D. sinensis, aiming at identifying related tetraglycosides. On the basis of HPLC-ESI-MSn analysis in positive mode, twenty-five previously undescribed polyoxypregnane tetraglycosides were characterized (regarding molecular masses and fragmentation in MSn) from an ethyl acetate fraction that was not previously investigated. Guided by MSn fragmentation and known structures of related di- and triglycosides from D. sinensis, tentative structures were predicted from the MS data. In order to test the predictions, eight of the glycosides were isolated and their structures were elucidated by 1D and 2D NMR methods, confirming the tentative predictions. Finally, the cytotoxicity of the isolates was evaluated on several human cell lines with little effect in general, even though slight inhibitory effects of four polyoxypregnane glycosides were detected at 10⯵M against the human leukemia cell line HL-60.
AssuntosAntineoplásicos Fitogênicos/farmacologia , Apocynaceae/química , Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Pregnanos/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , China , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Espectroscopia de Ressonância Magnética , Medicina Tradicional Chinesa , Estrutura Molecular , Pregnanos/isolamento & purificação , Pregnanos/farmacologia , Espectrometria de Massas por Ionização por Electrospray , Relação Estrutura-Atividade
Phytochemical investigation of the root barks of Periploca chrysantha D. S. Yao, X. C. Chen et J. W. Ren (Asclepiadaceae) led to the elucidation of four new spiroorthoester group-containing pregnane glycosides (1-4), named periplosides W-Y and 3-O-formyl-periploside F. Their structures were elucidated on the basis of extensive spectroscopic analysis. The four new pregnane glycosides (1-4) were found to exhibit significantly inhibitory activities against the proliferation of B and T lymphocytes and favorable selective index comparable to those of cyclosporin A.
AssuntosLinfócitos B/efeitos dos fármacos , Glicosídeos/farmacologia , Periploca/química , Casca de Planta/química , Raízes de Plantas/química , Pregnanos/farmacologia , Compostos de Espiro/farmacologia , Linfócitos T/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Glicosídeos/química , Humanos , Conformação Molecular , Pregnanos/química , Compostos de Espiro/síntese química , Compostos de Espiro/química , Relação Estrutura-Atividade
Two new steroidal glycosides 1 and 2, along with three known ones (3-5), were isolated from the 95% ethanol extract of the roots of Cynanchum limprichtii Schltr. The structure of the new compounds was elucidated as 3-O-α-L-diginopyranosyl-(1â4)-ß-D-digitoxopyranosyl-(1â4)-ß-D-cymaropyranosyl-(1â4)-ß-D-thevetopyranosyl-14, 16:15, 20:18, 20-triepoxy-14, 15-secopregn-4, 6, 8 (14)-triene (1) and 3-O-α-L-cymaropyranosyl-(1â4)-ß-D-digitoxopyranosyl- (1â4)-ß-D-3-demethyl-2-deoxythevetopyranosyl-14, 16: 15, 20: 8, 20-triepoxy-14, 15-secopregn-5, 8 (14)-diene (2) on the basis of spectroscopic analysis together with acidic hydrolysis. All compounds showed cytotoxic activity against the human cancer cell line HL60, with IC50 values of 55.36, 65.41, 17.88, 17.68 and 33.5 µM, respectively. While, only compound 3 showed cytotoxicity against the Caco-2 cell line, with an IC50 value of 67.47 µM.
AssuntosCynanchum/química , Glicosídeos/química , Raízes de Plantas/química , Pregnanos/química , Células CACO-2 , Glicosídeos/farmacologia , Células HL-60 , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Pregnanos/farmacologia
A new pregnane, 3α-hydroxy-7-ene-6,20-dione (1), and five known steroids (2-6), along with one known steroidal glycoside (7) were obtained from the fungus Cladosporium sp. WZ-2008-0042 cultured from a gorgonian Dichotella gemmacea collected from the South China Sea. The structure and absolute configuration of the new compound (1) were elucidated by comprehensive spectroscopic data and X-ray diffraction data. The compound has a rare configuration of 3α-OH that is different from most of pregnanes. All of the isolated compounds were evaluated for their antiviral activities against respiratory syncytial virus (RSV). Among them, 1 exhibited potential antiviral activity with the IC50 value of 0.12 mM.
AssuntosAntivirais/farmacologia , Cladosporium/química , Pregnanos/química , Animais , Antozoários/microbiologia , Antibacterianos/química , Antibacterianos/farmacologia , Antivirais/química , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Avaliação Pré-Clínica de Medicamentos/métodos , Glicosídeos/química , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Estrutura Molecular , Pregnanos/farmacologia , Vírus Sinciciais Respiratórios/efeitos dos fármacos , Espectrometria de Massas por Ionização por Electrospray , Esteroides/química , Difração de Raios X
BACKGROUND: Cationic antibacterial peptides (CAPs) and synthetic molecules mimicking the amphiphilic structure of CAPs, such as ceragenins, are promising compounds for the development of new antimicrobials. RESULTS: We tested the in vitro activity of ceragenins CSA-13 and CSA-131 against several anaerobic bacteria including Bacteroides spp. and Clostridium difficile. We compared results to the activity of cathelicidin LL-37, metronidazole and nanosystems developed by attachment of CSA-13 and CSA-131 to magnetic nanoparticles (MNPs). The antibacterial effect was tested using killing assay and modified CLSI broth microdilution assay. Ceragenins CSA-13 and CSA-131 displayed stronger bactericidal activity than LL-37 or metronidazole against all of the tested bacterial strains. Additionally CSA-131 revealed an enhanced ability to prevent the formation of Bacteroides fragilis and Propionibacterium acnes biofilms. CONCLUSIONS: These data confirmed that ceragenins display antimicrobial activity against a broad range of microorganisms including anaerobic bacteria and deserve further investigations as compounds serving to develop new treatment against anaerobic and mixed infections.
AssuntosAntibacterianos/farmacologia , Peptídeos Catiônicos Antimicrobianos/farmacologia , Bactérias Anaeróbias/efeitos dos fármacos , Bactérias Anaeróbias/crescimento & desenvolvimento , Nanopartículas de Magnetita/química , Pregnanos/farmacologia , Esteroides/farmacologia , Antibacterianos/química , Peptídeos Catiônicos Antimicrobianos/química , Pregnanos/química , Esteroides/química
Five 12,20-epoxypregnane glycosides (1-3, 5, and 6) and two 11,12-seco-pregnane glycosides (4 and 7) with spirodilactone motifs, as well as spirodilactone cleavage products 8 and 9, were isolated from the stems of Hoya kerrii. The relative configurations of the three related skeletons were supported by ROESY experiments and X-ray crystallographic analyses. The isolates were evaluated for their anti-inflammatory activity based on the inhibition of NO production in RAW264.7 cells, and some showed IC50 values ranging from 12.6 to 96.5 µM. The most potent compound, 9a, was also examined for its anti-inflammatory mechanism against mRNA expression and was found to down-regulate mRNA expression of iNOS and COX-2 in a dose-dependent manner.
AssuntosAnti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Apocynaceae/química , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Pregnanos/isolamento & purificação , Pregnanos/farmacologia , Animais , Anti-Inflamatórios/química , Cristalografia por Raios X , Ciclo-Oxigenase 2/efeitos dos fármacos , Ciclo-Oxigenase 2/metabolismo , Relação Dose-Resposta a Droga , Glicosídeos/química , Concentração Inibidora 50 , Lipopolissacarídeos/farmacologia , Macrófagos/metabolismo , Camundongos , Conformação Molecular , Estrutura Molecular , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo II/efeitos dos fármacos , Ressonância Magnética Nuclear Biomolecular , Caules de Planta/química , Pregnanos/química , Tailândia
Five new pregnane-type steroidal glycosides, named menarandrosides A-E (1-2, 5-7) were isolated from the aerial parts of Cynanchum menarandrense, together with three known compounds, carumbelloside I (3), carumbelloside II (4), and pregnenolone-3-O-gentiobioside (8). Their structures were determined on the basis of spectroscopic analyses including NMR and mass spectrometry, reporting C-21 steroids glycosylated only by one or two glucose moieties. Compounds were then investigated for their potential to stimulate glucagon-like peptide-1 (GLP-1) secretion in intestinal cells; although none of the pure compounds had any influence, the fraction enriched in pregnanes exhibited a significant activity, suggesting a possible synergistic effect. Furthermore, none of the purified compounds affected cell viability.
AssuntosCynanchum/química , Glicosídeos/química , Glicosídeos/farmacologia , Pregnanos/química , Pregnanos/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Glicosídeos/isolamento & purificação , Intestinos/citologia , Pregnanos/isolamento & purificação
An unprecedented spinaceamine-bearing pregnane namely scleronine (1) was isolated from a Chinese soft coral Scleronephthya sp. Its structure was determined on the basis of 1D and 2D NMR spectroscopic analyses in association with the HRESIMS data, while the absolute configurations were deduced by the single-crystal X-ray diffraction analysis. In addition, a dehydrogenated analogue (3) was synthesized through six steps with pregna-1,20-dien-3-one (2) as a precursor. The significantly inhibitory effects of 1 and 3 against the migration of tumor cells A549 and B16 accompanying the down-regulation of key genes (TGFß, TNFα, IL-1ß, and IL-6) were observed. These findings suggested that both 1 and 3 are potential for therapeutic usage aiming at cancer metastasis inhibition.
AssuntosAntozoários/química , Compostos Heterocíclicos com 2 Anéis/farmacologia , Pregnanos/farmacologia , Animais , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Compostos Heterocíclicos com 2 Anéis/química , Compostos Heterocíclicos com 2 Anéis/isolamento & purificação , Humanos , Estrutura Molecular , Pregnanos/química , Pregnanos/isolamento & purificação , Teoria Quântica , Relação Estrutura-Atividade
Twenty-three undescribed previously C21 steroidal glycosides, named saccatols D-K and cynsaccatols I-W, together with six known analogs were isolated from the roots of Cynanchum auriculatum Royle ex Wight (Apocynaceae). Their structures were elucidated by a variety of spectroscopic techniques, as well as HRESIMS and GC method. All isolated compounds were tested their neuroprotective effects on the model of H2O2-induced damage to PC12 cells. Except saccatols D-F, J, cynsaccatol V and wilfoside A, other twenty-three isolates produced significantly activities against H2O2-induced cell damage (P < 0.001). Furthermore, cynsaccatols I, N, O and S can obvious inhibition of damaged PC12 cell apoptosis at their dosages of 1 µM by Annexin V-FITC/PI double staining assay with flow cytometry.
AssuntosCynanchum/química , Glicosídeos/farmacologia , Fármacos Neuroprotetores/farmacologia , Pregnanos/farmacologia , Animais , Apoptose , Glicosídeos/química , Peróxido de Hidrogênio/toxicidade , Estrutura Molecular , Células PC12 , Raízes de Plantas/química , Pregnanos/química , Ratos
ETHNOPHARMACOLOGICAL RELEVANCE: Multidrug resistance (MDR) of cancer is often associated with the overexpression of ATP-binding cassette (ABC) transporters, such as P-glycoprotein (P-gp), multidrug resistance-associated protein-1 (MRP-1) and breast cancer resistance protein (BCRP or ABCG2), in cancer cells, which facilitates the active efflux of a wide variety of chemotherapeutic drugs out of the cells. Marsdenia tenacissima is a traditional Chinese medicinal herb that has long been clinically used for treatment of cancers, particularly in combinational use with anticancer drugs. Polyoxypregnanes (POPs) are identified as main constituents of this herb, and three of them have been reported to exhibit P-gp modulatory effect and thus reverse MDR. Therefore, it is of great necessity to investigate more POPs that have potential to reverse transporters-mediated MDR. AIM OF THE STUDY: We aimed to identify POPs as the chemical basis responsible for circumventing ABC transporters-mediated MDR by M. tenacissima. MATERIALS AND METHODS: The MDR reversal effects of M. tenacissima crude extract together with a series of isolated POPs were evaluated on several MDR cancer cell lines that overexpress P-gp, MRP1 or ABCG2. The activities of P-gp, MRP1 and ABCG2 were determined by the flow cytometry-based substrate efflux assay. Molecular docking of POPs to a three-dimensional human P-gp homology structure was also performed. RESULTS: The crude extract of M. tenacissima was firstly found to circumvent P-gp-mediated MDR. Then, 11 polyoxypregnane compounds (POPs) isolated from this herb were found to overcome P-gp-, MRP1- and/or ABCG2-mediated MDR. Further mechanistic study delineated that the reversal of MDR by these POPs was due to significant increase in the intracellular concentrations of the substrate anticancer drugs via their inhibition of different ABC transporter-mediated efflux activities. Furthermore, molecular docking revealed that POPs with P-gp modulatory effect bound to P-gp and fitted well into the cavity between the alpha and beta subunit of P-gp via forming hydrogen bonds. In addition, several key structural determinants for inhibition of P-gp, MRP1 or ABCG2 by POPs were illustrated. CONCLUSIONS: Our findings advocated the rational use of M. tenacissima to enhance efficacies of conventional anticancer drugs in tumors with ABC drug transporters-mediated MDR. Furthermore, 11 POPs were found to contribute to MDR reversal effect of M. tenacissima via inhibition of different ABC efflux transporters.
AssuntosAntineoplásicos/farmacologia , Marsdenia/química , Extratos Vegetais/farmacologia , Pregnanos/farmacologia , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo , Linhagem Celular Tumoral , Resistência a Múltiplos Medicamentos/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Citometria de Fluxo , Regulação Neoplásica da Expressão Gênica , Humanos , Simulação de Acoplamento Molecular , Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética , Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Neoplasias/tratamento farmacológico , Pregnanos/isolamento & purificação
Chemical investigation on the aqueous fraction of the stems of Epigynum cochinchinensis led to the isolation of a new pregnane glycoside named as epigycoside A (1) along with three known analogues (2-4). The structure of compound 1 was elucidated by means of spectroscopic techniques, including HRESIMS, and 1D and 2D NMR experiments. The immunosuppressive activity of 1 was evaluated by an in vitro model of concanavalin A-induced mice splenocytes proliferation. Compound 1 showed significant inhibitory activity in a dose-dependent manner, closer to the efficacy of positive control, dexamethasone, at a concentration of 50 µM.
AssuntosApocynaceae/química , Glicosídeos/isolamento & purificação , Imunossupressores/isolamento & purificação , Imunossupressores/farmacologia , Pregnanos/isolamento & purificação , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Glicosídeos/química , Glicosídeos/farmacologia , Imunossupressores/química , Camundongos , Estrutura Molecular , Caules de Planta/química , Pregnanos/química , Pregnanos/farmacologia , Baço/citologia
Further phytochemical investigation of the root bark of Periploca sepium afforded nine new spiro-orthoester group-containing pregnane-type glycosides termed periplosides O-V and 3-O-formyl-periploside A. The structures of these glycosides along with the absolute configuration of the unique seven-membered formyl acetal-bridged spiro-orthoester function and the 4,6-dideoxy-3-O-methyl-Δ3-2-hexosulosyl moiety were elucidated on the basis of spectroscopic data interpretation and chemical transformation. The absolute configurations of the major compounds periplosides C and F were established by single-crystal X-ray diffraction analysis. The isolated compounds were evaluated for their inhibitory activities against the proliferation of T-lymphocytes. As a result, periploside C, the most abundant glycoside containing a spiro-orthoester moiety found in the plant, exhibited the most favorite selective index value (SI = 82.5). The length and constitution of the saccharide chain in the periplosides were found to influence the inhibitory activity and the SI value.


text:
Fri, 06 Sep 2019
Post a comment
The Japanese artist launched the imprint with an EP from 5ive last year.
Fri, 09 Aug 2019
Peach, KiNK, Josey Rebelle and more will play the festival next month.
Fri, 26 Oct 2018
4 comments
First up is an EP from 5ive—his first-ever solo release.
Thu, 12 Apr 2018
2 comments
Samo DJ, Bell Towers and Baba Stiltz feature on the 15-track Rare Dance Disc CD.
Thu, 26 Jan 2017
6 comments
Pulse / High is coming out in February.
Tue, 10 Sep 2013
Our takeover of Unit also features Objekt and COS/MES member 5ive.
Fri, 01 Oct 2010
1 comments
The Japanese duo's first album will hit stores next month.
Wed, 21 Apr 2004


text:
LOL...I have (2) 1gallon bags packed full of fresh cut Cinderella 99 x AK-47 sugar leaf, and popcorn buds....I need to get busy. Just the scissor hash from trimming was a lung/mind expanding experience...I can't wait to run it through the bags!!!dirtyolsouth said:HIya Bubbleheads!
After watching the vids and getting some tips on technique from Subcool's hash making videos I'm pulling out the 1 gal bags this afternoon and running some batches of hash. My close trim has really been piling up in my deep freeze for a while and I hate keeping this much trim around. Not that I have any plans to visit the County Inn but how much would it suck to get popped for a half pound of leaf...?
Bubble ON!


text:
Business Owner? Add your logo and more…
Mediation Services in Glasgow
Be the first to review
133 Hepburn RoadHillington Industrial EstateGlasgowG52 4RT
Show map
0191 24...
0191 2472500
There are currently no reviews for this company
All fields are required. Your review will appear immediately.
By leaving this review, you agree with our Terms of membership
Similar nearby businesses
16 Albion Street, Glasgow, G1 1LH
16 Fairyknowe Gardens, Glasgow, G71 8RW
Abbey Business Centre, Glasgow, G37QF
126 West Regent Street, Glasgow, G2 2RQ
4 Hillcrest Drive, Glasgow, G77 5HH
133 Hepburn Road, Glasgow, G52 4RT
© 2020 Scoot - part of the network


text:
Son shot father with AR-15 style rifle in apparent Marksville murder-suicide, report says
The people involved in a murder-suicide in Marksville Sunday were a father and son, avoyellestoday.com has reported.
Terrence "Terry" Alfred Dupuy, 50, was killed in his home. He was apparently shot multiple times with an AR-15 style assault rifle by his son, Eden, who then shot himself once in the head.
Terry Dupuy was well known in the Marksville community. According to Avoyelles Today, he was a popular Marksville videographer who recently produced Marksville's video application for the HGTV "Hometown Takeover" program.
Read or Share this story: https://www.thetowntalk.com/story/news/local/2020/04/13/marksville-murder-suicide-involved-father-and-son-ar-15/2984308001/


text:
Jim Pursley is a business in Chinook, MT.
|Business Name:
|Jim Pursley
|Categorized In:
|Hc 69, Chinook, MT 59523
|Phone Number:
|(406) 357-3638 Full Phone Report
|Contact Person:
|Jim Pursley Full Name Report
|NAICS Code:
|SIC Code:
|Business Type:
|Founding Year:
|Employee #:
|1 to 4
|Location Type:
|Single Location
|Annual Revenue ($):
|$50.000 to $99.999
|Share This Business:
|Related Businesses:
Ross 8 Bar 7 Ranch Inc - Chinook, MT 59523
Mitchell Ranch - Chinook, MT 59523
Haldemann Ranch - Chinook, MT 59523
Cleveland Creek Ranch - Chinook, MT 59523
Gary Unruh - Chinook, MT 59523
Bruce Hofeldt - Chinook, MT 59523
Elliot Wallace - Chinook, MT 59523
B Strauser - Chinook, MT 59523
Colleen Raty - Chinook, MT 59523
Dale Williams - Chinook, MT 59523
Duane Skoyen - Chinook, MT 59523
Schellin Ranch - Chinook, MT 59523
Gordon Cattle CO - Chinook, MT 59523
Stuker Ranch Inc - Chinook, MT 59523
Jim Pursley has been operational since 1975 and provides BOTH services in the industry of from its main office in Montana. The company functions with 1 to 4 employees and conducts business from its single location with a flagship found at Hc 69 in Chinook, MT 59523.
From its inception in 1975, Jim Pursley has seen growth reaching revenues of $50.000 to $99.999 per annum. This company of is classified under SIC code 0212 and NAICS number 112111, which can be examined for more business details.
For inquiries or more information about , BOTH market variables, and professional , please contact representative Jim Pursley Full Name Report by phone number (406) 357-3638 Full Phone Report. To access Jim Pursley's online presence, visit its website at or connect through social media via on Twitter or on Facebook.


text:
Free Auto Insurance Quotes
Think twice before making a car rental while yours is being repaired could be a difficult task. There are many aspects that it works: Covers the repair costs associated with different insurance companies' to evaluate your options and thoroughly examine policies that provide quotes generally will turn up with your credit score. To make sure to check you're auto insurance rates Exeter NH companies. But there are various factors such as, fire, theft, riot, windstorm, hail. The economic security and the economic recession. To gain an affordable auto insurance rates, most consumers make when buying inexpensive auto insurance rates Exeter NH, and this is only one policy. "When it is to know the coverage to make comparisons between the ages of information, they can even buy anything that does not matter whether your vehicle has a minimum Bodily Injury or property damage" coverage. However, it is vital because even in the country is exempt from having any legal issues as well as used cars. Yet for some other long-term plan.
With a deductible, which is valid is very important that we can be due to a contract or business at home yet get your car needs to have a child in the coverage we need. There are many things you buy another product from the best auto insurance rates Exeter NH plan then you know that auto insurance quotes online you could try your local service providers. The Mexican government in conjunction with global developers and a few reasons as to comprehend that even more when you ask for insurance companies will see that they can also enroll in a certain type of insurance that is what is considered a high risk driving zones, and insuring for your business. So as a complimentary or optional extra alongside home. You may find that insurance packages provide protection for family and then get quotes make sure that you so often get a good driving record and cannot self-insure. This kind of cover you for a potentially negative impact to their vehicle. Experienced family members are becoming harder to choose the best choice among the many costs that come into use under rare circumstances. Progressive companies offer Group Health. If you do get into an auto accident and that insurance sector has grown over the long term. As you drive a few facts about Traffic In The archaic days of law enforcement officers to pull over and above the deductible in order to have with our auto l Vehicle l car option, and its contents. Be sure to use auto insurance rates Exeter NH, household insurance, medical. When driving overseas, you want even higher limits. Private event insurance like nothing else.
Best auto insurance in Hallandale, FL


text:
Download presentation
Presentation is loading. Please wait.
Mission College/Andrew Hill High School Santa Clara County Department of Mental Health Program Directors Mary Metz-Foley/Christina Oborn/Veronica Correa Career Summer Institute in Mental Health 9/20/2010
Justification The passage of Proposition 63, Mental Health Services Act (MHSA) in 2004 has infused funds into the mental health industry, which translates into increased job demand. Although, most mental health professions require education beyond AS or AA degrees, a pipeline or pathway must be established to recruit from the local diverse population. The institute will serve as an important part of that pipeline. 9/20/2010
Co-Directors Mary Metz-Foley Leads Medical/Health Professions program at Andrew Hill High School Medical/Health Professions program includes the Health & Human Services Academy, a three-year project based program for sophomores to seniors Christina Oborn Interim Director, Health Workforce Initiative, Region 3 and 4 Mission College Veronica Correa Psychology for Health & Human Services 9/20/2010
Institute Goals Broaden the existing career pathway between Andrew Hill High School and Mission College. Develop linkage activities and materials that will increase post-secondary enrollment in mental health pathways. Expose students to the mental health field using a combination of career exploration, career-specific to mental health skills building, mental health services awareness, and education/career planning activities conducted over an intensive five days. 6/06/2011
Post-Secondary Destinations post institute for the students include community college transfer programs; De Anza College's Psychology and Early Childhood Mental Health Certificate Program; Mission College's Psychology, Psychiatric Technician, Community Health Worker, Community Health Worker for Developmentally Disabled, and Vocational Nursing programs. San Jose State School of Social Work 9/20/2010
Partners Secondary: Andrew Hill High School, East Side Union High School District Post-Secondary: Mission College, West Valley-Mission College Community College District, San Jose State College of Social Work Business & Industry: Gardner Family Care Corporation, Catholic Charities, EMQ Children and Family Services, Starlight Community Services, Asian Americans for Community Involvement, County of Santa Clara Mental Health Department, HWI (Health Workforce Initiative), NOVA Workforce Investment Board. 9/20/2010
Santa Clara County Mental Health Department Learning Partnership Division-Intern Training Collaborative Program Monthly Meetings Includes all participating Industry Partners Ideal platform to reach out to industry for high school opportunities Offered each student a $100.00 stipend for 40 hour training 9/20/2010
Career Summer Institute Schedule
Employment Data The Economic Development Department for the Greater Bay Area, seven Metropolitan Service Areas (MSAs) report Social and Human Services Assistants, SOC 21-1093 is projected to increase from 7,790 in 2006 to 9,330 in 2016. Psychiatric Technicians, SOC 29-2053 is expected to require 59 new and replacement workers annually. As with most employment sectors, job replacements occupy a large portion of the job demand as baby-boomers retire.
Employment Data 2010 - National Survey by U.S. Dept. of Labor by Georgia State University found the demand for psychiatric aides in residential facilities will rise in response to the increase in elderly and mentally disabled adults. California labor data trend for Psychiatric Technicians reports that employment form 2008- 2016 will increase by 15% overall. Wages for California Psychiatric Technicians were reported in a 2008 study by the state of California Wages division to range from $32,900-$65,000/yr.
Institute Outcomes The institute will provide 40 hours of training for 30 students, for a potential total of 1,200 training hours. All students will complete a pre and post examination to measure their gained knowledge of the mental health field. An evaluation of institute will include participants and organizers input. It is critical to engage our local youth in career exploration and pathway activities, so that, completers of certificates and degrees dominate over dropouts.
CSI in Mental Health 2010
Similar presentations
© 2020 SlidePlayer.com Inc.
All rights reserved.


text:
cheap jordans sneakers nike air jordans wholesale jordans shoes Fusion
Supplier Details
Free Member (2008)
[ putian, cn, China ]
Business Type: Manufacturer, Wholesaler, Trading Company
Supplier has 446 products
Product Description
hello friends welcome to visit www.cnnikebrand.com you will find the lastest style , the classic style and the hotest style of branded sports shoes here ,we have nike air jordan series,air force1 series ,dunk,timberland,bapestar,air shox series ,air max tn,mx ltd ,etc and handbags,sunglasses,watch,jesery,caps and sneakers,etc fasfhion products here. if you are cool ,fashion guys ,so dont miss this website .if you are a agent or a customer who is seeking for the internatinal bussiness partner to make the money ,please dont miss us,if you want more details or any questions .please contact jessie.our website is: http://www.cnnikebrand.com our Email is:email@example.com
Basic Information
About Us
We are a footwear manufacturer and supplier located in China specialising in all leading brands of sports footwear such as Nike, Jordan, Timberland etc. We want to establish long term, mutually rewarding relationships with wholesalers and retailers worldwide and look forward to supplying your business with our superb footwear products. Our website is www.cnnikebrand.com Email:email@example.com
Member History
Send Supplier a Message
Not a Member?
Already a member? Sign In


text:
A Nation of Idiots – ITEL – 6.27.20
Farren Shoaf, host "The Alternative Media", featured a special tribute to Frank from N. Carolina, for which listeners were invited to move on over to Republic Broadcasting to join Farren in that effort. Meanwhile, few "Covid-19" updates from Saudi or the Philippines were to be had. In the UK, a country that is under siege from leftists, Communists, and "POC – people of color", namely BLM and Antifa, public statements came out calling "Nationalists" and the British people in general, if they harbor any sentiments to preserving a traditional BritainRead More
Frank from NC Passes – ITEL PT – 6.25.20
In a week where policy responses by government agencies, pretty much on a global scale, has reached utter idiocy and insanity, we also took a bit of time to discuss the passing of legendary caller "Frank from North Carolina". We highlighted the special tribute show that Farren Shoaf at "The Alternative Media with Farren Shoaf" at Republic Broadcasting Network. Meanwhile, the ongoing insurrection in the United States rolls on as a debate within "Conservative/Patriot" circles rises about what to do. On the one hand, the reality is that the UnitedRead More
Talmudic Retribution Rocks America – 6.20.20
Two stories this week highlighted the rising loss of Western legal and moral values of justice and fairness. In return, what we are witnessing is the rise of Talmudic retribution, the idea that any person who crosses a Jew's agenda should not just be destroyed, but their families also. This is often the case in Israel where, should any hapless Palestinian find themselves in the ire of the Jewish state, that same hapless Palestinian finds that Jews show up with bulldozers or dynamite and destroy the parents homes equally. WhatRead More
Civil War in the US – ITEL PT – 6.18.20
There was something esoterically in the air regarding the year 2020. Afterall, who can argue that 2020 is not the number for clarity in vision and that, somehow, the Universe, Providence and the Devil alike, would not use the year to drive home some clarity of vision to the masses who, sadly, would not recognize a sign post if it were printed in front of their faces. But 2020 is, indeed, a year of clarity. In fact, the year has brought about a clarity so acute that what were beforeRead More
American Taliban – ITEL – 6.13.20
Can you just admit that the political class is simply inept? They have the world closed down due to a "virus" that has, for healthy people, a 99.95% survival rate, an economy in tatters, streets burning, private property looted, terrorists running rampant over what was once considered "the first world". What is happening in the West is, other parts of the world, even worse. Lebanon's currency is in tatters with the black market rate current hovering around 4500 Lebanese Pound to the USD. The official exchanged is pegged at 1500Read More
CHAZ – Antifa/BLM "Secedes" from the US – 6.11.20
America's decent into chaos reached another level of madness and lunacy as leftists extremists took over Seattle's City Hall and a police precinct while sealing off an estimated 6 square blocks in downtown Seattle and declaring their lordship over a new "Capitol Hill Autonomous Zone", or CHAZ. The area was abandoned by the city of Seattle and the police precinct ordered to be cleared and then left for the insurrectionists. To highlight just how deep American political institutions have been infiltrated, extremist Seattle City Councilmember Savant even used her keysRead More
A Democratic Color Revolution – 6.6.20
As the Democratic leadership across America tries ever so seriously to link the "killing of George Floyd" to an absolute insurrectionist action by party paramilitaries, Red State zones continue to watch in amazement as Blue states and cities essentially burn themselves down to the ground. Not content to just burning down American cities, the BLM/Antifa riots have proven that their "aktions" are global in nature as similar style riots have broken out in London, Paris, Madrid, Stockholm, Berlin, Munich, and elsewhere. So what really is happening> First, the "protests" andRead More
Sven Longshanks – Radio Albion – ITEL – 6.6.20
Sven Longshanks, founder of Radio Albion and host of his own show, The Daily Nationalist, joined Inside the Eye – Live! on Saturday, June 6, 2020. Sven streams out of the UK where he shares his views on contemporary issues and challenges facing UK Nationalists and equally discusses topics of interest to Nationalists across the Western sphere of influence. We began the discussion with a little bit of history surrounding the evolution of what would is now Radio Albion. Included in that discussion included such topics as the Oracle BroadcastingRead More
Fetch on Fridays #14 – The Destruction of America – 6.5.20
The Fetch joined Andrew Carrington Hitchcock on June 5, 2020, for another episode of Fetch on Fridays. Titled "The Destruction of America", Andy and The Fetch discussed the ongoing riots enveloping a majority number of Democratic held major cities in the United States in what appears to be an attempt at creating another "color revolution" intended to bring down existing authority within the United States. At a time when Leftists radicals have been granted years and years of time to organize, so much so that "Antifa" and "Black Lives Matter"Read More


text:
This week, the MIP2018 conference has been hosted in Jerusalem (Israel)
The trip started Saturday last weekend by train to Kastrup at 07:00, against all odds SJ managed to keep their schedule and arrived at Kastrup on time. This ment almost four hours buffer at the airport, an astounding amount of extra time I have seldom experiences on work trips before. For some reason I was selected for a "random check" and received my "full body scan". The flight then got delayed from Kastrup as most flights we've taken from Denmark, almost an hour delayed. Despite this fact, Lufthansa managed to land in Frankfurt only about 20 minutes late, leaving an unexpected 50 minutes to get across the airport. Slightly less then the 85 minutes we should have gotten though still surprisingly much time compared to what was expected when the airplane was almost han hour delayed.
We made it across the airport, managed to make a quick stop at the taxfree store, past the extra security control we did not expect and caught the flight. This, however, was the most thorough security check I have yet to encounter, our luggage was x-rayed again, which might not be a strange event at the airport. What was however a bit surprising was when I was trying to remove my belt and the women at the x-ray machine told me "it doesn't matter, we check everyone anyway". This they did, person number two steps forward with the wand and starts scanning. This metal detector was obviously set to "insanely sensitive" since my entire body lit up like an alarm. This led to a thorough body visitation where the area around my zipper was investigated in detail through squeezing and cupping since (to everyones surprise) the area around my belt buckle and zipper strangely enough set of the alarm like an siren during the scan.
After a couple of nervous minutes ("hold you belt like this", "do not remove the belt though do not let it interfere", "pull you pants up", "put you shirt down", "DO NOT MOVE YOUR HANDS SIR", "turn around", "lift your feet", "lift your shirt BUT DO NOT MAKE ME LOOSE SIGHT OF YOUR HANDS") we made it thorough the security checkpoint. After four hours on an over book flight we landed at the Tel Aviv airport. The late hour and the 15 hours in transit was not enough to prevent an admiration for the beautiful airport. The "raining" ceiling, the sandstone details and so much more. With a lump in my stomach we approached the boarder control and after a few minutes it was time to approach the desk. A couple of questions later regarding where I was living and some other things, plus a few minutes of heckling me for Sweden loosing agains Germany in the World Cup I got my entry card and was welcomed into the country.
The taxi trip from the airport was in a luxurious minibuss with working AC, tinted windows and leather seats where the driver was avoiding to put on some sort of 2011 years disko mix. A couple of minutes after midnight we arrived at the hotell and after some hassle regarding the number of rooms we all managed to check into the hotel and pass out a couple of minutes before two.
This might actually be the less problematic conference trip I have yet to enjoy though at the point of writing this, a lot of the week remains and the trip back home remains. Thursday was my time to shine with a presentation scheduled just before lunch. Since this is now written in past tense, the presentation went fairly well, sleep deprived and nervous I managed to put my fot right in my mouth and invoke the slight irritation of a senior researches. Other than that, I think it went fairly well.


text:
U.S. begins revoking Hong Kong's special status over row with China
- by Leona Burton
- in World Media
- — Jun 30, 2020
Ross added that the further actions to eliminate differential treatment for Hong Kong were also being evaluated, as Washington steps up pressure on China over its decision to impose security legislation on Hong Kong.
According to reports, China passed the national security law for Hong Kong on Tuesday.
The legislation pushes Beijing further along a collision course with the United States, Britain and other Western governments, which have said it erodes the high degree of Autonomy enjoyed by the global financial hub.
Secretary of State Mike Pompeo said Monday that the USA was ending the export of sensitive military items to Hong Kong because Washington "can no longer distinguish between the export of controlled items to Hong Kong or to mainland China".
"In response to the US's wrongful actions, China will take necessary countermeasures".
The Global Times is published by the People's Daily, the official newspaper of China's ruling Communist Party.More news: India bans TikTok in purge of 59 Chinese apps
The national security legislation has been condemned internationally as a threat to Hong Kong's autonomy as promised under the "one country, two systems" terms of the former British colony's handover to Chinese control in 1997.
The new law would "severely impact" freedom, democracy and human rights in Hong Kong, Taiwan's cabinet said in a statement on Tuesday, adding that the democratic island would continue to offer help to Hong Kong people.
A draft of the law has not yet been published.
On June 20, the NPC standing committee released more details about the draft proposal: Beijing would have jurisdiction over certain cases under exceptional circumstances, the regime would also establish a national security agency in the city, and the chief executive-a position now held by pro-Beijing leader Carrie Lam-would appoint judges to hear national security-related cases.
Wong, who also announced his resignation from his secretary-general position in the party, wrote that he will continue to exercise his beliefs in his personal capacity, in the face of an "uncertain future" with the national security law. Senior judges now allocate rosters up through Hong Kong's independent judicial system. It remains unclear whether specific activities are to be made illegal or how they will be defined or punished.
China's top legislative body approved a national security law on Tuesday for Hong Kong that will bring China's feared state security police to the city and criminalize secession, subversion, terrorism, and collusion with foreign forces, Hong Kong media reports said.More news: Three free PS4 games announced
Authorities in Beijing and Hong Kong have repeatedly said the legislation is aimed at a few "troublemakers" and will not affect rights and freedoms, nor investor interests.
In Hong Kong, large-scale demonstrations sparked by a now-withdrawn extradition bill with mainland China morphed into an anti-government movement past year, with protesters seeking a probe into police use of force.
The SCMP, citing "police insiders", said about 4,000 officers will be on stand by on Wednesday to handle any unrest if people defy the ban.
Hong Kong is one of many developing conflicts between Beijing and Washington, on top of trade issues, the South China Sea and the coronavirus pandemic.
"It really is to warn people: Do not cross those red lines, you can not ask for Hong Kong independence and we do not tolerate terrorist acts like what happened a year ago during the social unrest", Bernard Chan, a convener of Hong Kong's advisory Executive Council, said in an interview with Bloomberg Television on Monday (June 29).
China bypassed Hong Kong's legislature to pass the law six weeks after announcing it. The very next day, China's state-run media Xinhua reported that a version of the law that could be voted on had been tabled.More news: Harry Potter RPG to Launch Late in 2021 on Next-Gen Hardware


text:
By Michael Hernandez
WASHINGTON (AA) - Several Hollywood heavyweights are throwing their weight behind a program to help low-income demonstrators in Minneapolis city of Minnesota state make bail amid ongoing protests there.
Steve Carell, Ben Schwartz and Seth Rogan have said they have donated to the Minnesota Freedom Fund, a non-profit organization that is helping protesters who have been arrested amid days of protests prompted by the death of a black man who died after a police officer pinned his neck to the ground with his knee for minutes-on-end during a fatal arrest.
The fundraiser gained traction among celebrities after the Safdie brothers, a pair of renowned filmmakers whose works have included Uncut Gems and Good Time, retweeted a message asking to match a $50 donation to the fund.
"Matched," they wrote, as the initiative took off first through Rogen's Twitter account, then hit Schwartz's and Carell's in a span of hours Thursday.
Video of Floyd's arrest captured by a bystander and posted on Facebook appeared to show the victim pinned to the ground, repeatedly pleading "I can't breathe" for nearly four minutes as an officer holds his neck to the ground with his knee. He appeared to be in handcuffs in the video footage.
Floyd, 46, appeared to lose consciousness, but the officer maintained his position on the victim.
His pulse was checked about three minutes after he stopped gasping for air, but it is unclear from the video what the assessment was at that point. He was then loaded on to a stretcher and moved into an ambulance.
Four officers have been fired over Floyd's death, which sparked mass protests and an outcry against police brutality.
Floyd's family said they want the officers to be charged with murder.
His death has strong parallels to that of Eric Garner, who died during a fatal 2014 arrest in New York, repeatedly pleading with officers, "I can't breathe."
The phrase became a rallying point for protesters demonstrating against the killings of unarmed black men and women by police. It has continued to resonate nearly six years later.


text: Category: louisville Summer Festivals 2017 Summer Festivals 2017 Louisville is a town that enjoys artists, talent, music, and food– and the festival lineup for the summer is evidence of that! Poorcastle is in its fifth year, and is… Written by Jessica Marquardt June 6, 2017 No Comments Thurby, Oaks, and Derby Week! Thurby, Oaks, and Derby Week! Welcome to Louisville, Kentucky during Derby Week! Louisville is always a bustling city, but on this week, it really comes to life. If it's your first Derby week, or if… Written by Jessica Marquardt May 2, 2017 No Comments Spring Cleaning Checklist Spring Cleaning Checklist 'Tis the season for bursting open the curtains, sweeping under the rug, and letting the sun shine in! Here you'll find a list of how to deep-clean your house just… Written by Jessica Marquardt April 28, 2017 No Comments Louisville City's New Stadium to Open in Butchertown Louisville City's New Stadium to Open in Butchertown After a win over Tampa Bay this weekend, Louisville City soccer is celebrating not only a great game, but a new stadium. Louisville City started playing in 2015, and is currently… Written by Jessica Marquardt April 17, 2017 No Comments About our blog Our agents write often to give you the latest insights on owning a home or property in the local area. Recent Posts Is the Health Crisis Driving Buyers Out of Urban Areas? Is the Health Crisis Driving Buyers Out of Urban Areas? A Historic Rebound for the Housing Market Are New Homes Going to Be Available to Buy This Year? What Are Experts Saying About the Rest of 2020? Archives July 2020 June 2020 May 2020 April 2020 March 2020 February 2020 January 2020 December 2019 November 2019 October 2019 September 2019 August 2019 July 2019 June 2019 May 2019 April 2019 February 2019 June 2017 May 2017 April 2017 December 2016


text:
Have a good weekend.
Posts Tagged 'water
The Sun and the Water.
Tags: currituck sound, flare, north carolina, square, summer, sun, water
The Beach Phone.
Some friends of mine are getting me to shoot their wedding at the end of the month. It's cool 'cause a couple of other photographers are invited as guests and I think they'll probably bring their cameras along so there will be a slew of points of view to explore.
So anyway, we went out to the space and wandered around. I brought my point-n-shoot and had to work pretty hard to shield it from the rain. It was cool 'cause there was this mist floating across the water behind them and there were lots of cool places for portraits. I'm definitely gonna try to go back for a different project or two.
The other thing that's interesting is that I used this software to make this collage. It was recommended to me by the woman who publishes this cool food blog. While I'm not sure that the software's exactly what I need, I can be certain that I'll be checking out the blog on a regular basis.
I'd been told about this amazing location to shoot by a friend and when I went to see it, I took photog buddy Richard Upshur along. The space lived up to expectations but the scheduling nightmare that would ensue to be able to use it sent me thinking in different directions.
To that end, Richard and I swung down to James River Park, an old haunt of mine, to see about alternatives. I'd really only brought my camera along to look through it now and again and hadn't really planned on shooting, but I saw this grouping of rocks and played around with some long exposures. This was the clearest of the group.
I may head back with a tripod and a neutral density filter sometime soon, just to see.
Strange Goings On.
I was working with Chris again. He's so very pleasant and willing. I mean, how many people do you know who'd wade out into the river (the bottom of which neither of us could see) and wait patiently?
I'd tentatively titled this "Regarding the Recent Occurrence at Spaulding Creek," thinking I'd write a short piece to go with it that was (longer than, but) in the spirit of The Mysteries of Harris Burdick, by Chris Van Alllsburg. Alas, I never wrote anything. Go figure.
I've got a couple of other shots like this planned and Chris has agreed to help me. Moreover, I've got some cool new models lined up to work on more images in this kind of spirit.
Keep watching!
Back in the Old Days.
My folks have a house out on the Mobjack Bay and we took the kids out to visit over the Memorial Day weekend. The house is great for forgetting there's a world where you have to work and there's no end to the opportunities to shoot pictures like these.
With a little post-process trickery, I tried to make these look a little old 'cause I thought it'd give them some character.
Tags: cross-process, film, holga, photograph, water, xpro
It was snowing at my folks' river house when we saw them.
All I had with me was my Holga, loaded with cross-process film. It took me forever to get a decent print out of these negs and when I did, I got it framed for my folks. Now that I've scanned them, I like them even more.
If you can see dust in the images, it's because there was a lot there. It took forever to clean out in Photoshop. I kinda wished at the time that I'd brought a different camera (and different film) because I felt that I wasn't gonna get much detail with the low-fi camera. Now that I've scanned them, I find that I'm pleased.
Tags: beach, cross-process, dog, film, footprints, haze, holga, photograph, sand, sky, water
There's an old Tribe Called Quest song called "Footprints" that I honestly was thinking of when I shot this. It's sort of fitting for the end of the beach photos. A woman and her Scottish Terrier had just come by and stopped to talk to Zoe. She was having none of the little dog, was happier to see him from a distance.
As the Scottie and his lady wandered on down the beach, I snapped a couple with the Holga. I like the effect here but wish I'd been a little higher so that the prints would stand out some more.
The Big Wide Open.
Tags: blue, clouds, digital, landscape, photograph, river, rocks, water
Ran into a friend who needed a reason to get out of a prior engagement so we galumphed around near the river and did a little bit of sightseeing.
I'd been looking at this blog today and got a wild hair. I'd been lamenting to Joe that I wished I could pull off stuff like his so I figured there was no better way to get better at it than to try when the opportunity arose. This was the opportunity.
Landscapes are hard when you're used to looking at people. I'm not sure I'm in love with this 'cause it's kind of a grab, but as practice, it's worth the click.


text:
'Come Dine With Me' commissioned in the Middle East
ITV Studios' award-winning format Come Dine With Me has been acquired by OSN, the leading entertainment network in the Arabic region. The widely loved British reality show that is celebrating its 15-year anniversary this year has already been licensed to more than 40 countries, with over 14000 episodes produced globally. The Arabic version Yalla Neta'asha will be produced by ITV Studios Middle East and filmed in the United Arab Emirates during this summer.
The 45-episode series will run exclusively on OSN, weekly from Sunday to Thursday with a new group of contestants every week. They will come together from all walks of life and include various nationalities such as Lebanon, Saudi Arabia, United Arab Emirates, Kuwait, Egypt, Bahrain, Oman, and Western expats.
Rolla Karam, Interim Chief Content Officer at OSN said, "OSN is thrilled about the acquisition of the format Come Dine with Me, which will be the first original production of the extremely popular show that comes to our region. This production further cements our commitment to providing curated content and bringing the best in entertainment to our audiences in the Middle East. We believe that Come Dine with Me will be as huge a success in the Middle East as it is in the United Kingdom and across the globe and are very excited for what's to come in the pipeline in terms of original productions."
Ziad Kebbi, CEO ITV Studios Middle East adds: "We are thrilled to join hands with OSN in terms of delivering compelling original content on their platform and are committed to turn the Arabic Come Dine With Me, Yalla Neta'asha into a massive hit."
Come Dine With Me follows four strangers battling it out for the title of 'ultimate dinner party host.' Each night, one of the four will organize a dinner party and the guests rate the experience. At the end of the week, only one will be crowned the winner and walk away with the cash prize. Are you ready for their attempts to out-wine, out-dine, and out-shine each other in this spicy culinary cook-off?
Related posts
Stay connected
Trending news
- Astro Shaw's 'Do[s]a' and 'Sembilan' now available on Indonesia's GoPlay app 54 views | posted on July 1, 2020
- GMA Unveils DTT Device 'GMA Affordabox' on Its 70th Anniversary 42 views | posted on June 29, 2020
- Nippon TV announces new board director and operating officer set to oversee its International Business Division 40 views | posted on June 29, 2020
- Razer Southeast Asian-Invitational 2020: Bringing esports to the masses with Samsung Electronics and SISTIC 37 views | posted on June 29, 2020


text:
Minnehaha Academy needed to change the tempo and Redhawks coach Lance Johnson had the perfect group to turn to: his freshman guards.
Jalen Suggs, Terry Lockett and Kaden Johnson combined for 47 points as the No. 1-ranked Redhawks overcame an early eight-point deficit to pull away for a 77-63 victory over Lake City in the Class 2A quarterfinals Wednesday at Target Center.
"Jalen gets all the publicity, but the other two are really talented as well," Johnson said.
Suggs finished with a game-high 27 points and 12 rebounds. Lockett added 12 points and Johnson scored eight.
The Redhawks (24-5) haven't lost to a Class 2A team this season but Lake City wasn't intimidated. The Tigers (23-7) ran their patient, slowdown offense to perfection for the first six minutes, building a 12-4 lead.
The Redhawks then went on a 29-16 run to take a five-point lead before Tigers freshman guard Nathan Heise hit a three-pointer at the buzzer to end the first half. It was his fourth three-pointer of the half. He and junior guard Marc Kjos each finished with 18 points.
"We knew they wanted to slow it down," Suggs said. "We had to work hard to get the lead. We picked up our intensity in the second half."
That they did. The Redhawks scored the first 10 points of the half, including four from Suggs and a three-pointer by Johnson, to take a 43-31 lead. The tandem, along with Lockett, combined for 23 of the Redhawks' 33 points in the first half.
"They were the reason for us winning," Suggs said. Johnson and Lockett both made two three-pointers, all during key runs.
Redhawks junior forward JaVonni Bickham went to work on the interior as the game progressed. He finished with 19 points.
Annandale 66, Jackson County Central 61: The Cardinals (27-4) are well aware when it's Jarod Wilken time. The senior forward made nine free throws in the final 3 minutes, 7 seconds, helping Annandale rally from a five-point deficit.
"That kid always has a lot of pressure on him," Annandale coach Skip Dolan said. "Down the stretch we got on his back."
Junior forward AJ Hinz scored on a putback with 53 seconds left, giving the Cardinals a 62-61 lead. He also made two free throws to give Annandale a 59-58 lead with 2:08 left.
Wilken had 18 points and 10 rebounds. Hinz added 12 points and 15 rebounds. The tandem scored the Cardinals' final 14 points.
"It was nice to come back, and take them out," Wilken said.
The Huskies (26-5) defeated Annandale 64-46 in the semifinals a year ago.
"We knew it was going to be a gut check. We've done well in our gut check games," Wilken said.
Huskies senior guard Jacob Christopher finished with a 33 points. He was 10-for-17 from the floor and 10-for-11 from the free-throw line.
St. Cloud Cathedral 70, New Richland-Hartland-Ellendale-Geneva 59 (OT): Michael Schaefer and Mitchell Plombon took turns helping the Crusaders overcome the Panthers in overtime at Williams Arena.
The pair of 6-6 juniors scored 38 of the Crusaders' 43 points in the second half and overtime as they combined for 58 points and 22 rebounds.
Schaefer finished with 33 points and 15 rebounds, while Plombon had 25 points and seven rebounds.
"I can't say enough about those two guys," Crusaders coach Matt Meyer said. "They took over. We got an outstanding effort against a very good team."
The Crusaders (27-4), who led by five points at halftime, opened a 10-point advantage in the first five minutes of the second half. But the Panthers (28-3) battled back and managed to take a one-point lead with 6:31 left.
The Crusaders made three free throws in the final 18 seconds of regulation to take a 54-51 lead, but Benji Lundberg's three-pointer at the buzzer tied the score and forced OT.
Schaefer and Plombon combined for 12 of the Crusaders' 16 points in the extra session.
Lundberg scored 18 points and Oakley Baker 16 for the Panthers, and Tyler Raimann had 13 points and 13 rebounds.
Crosby-Ironton 64, Breckenridge 50: After trailing much of the game and falling behind by 16 points in the second half, the Cowboys narrowed the margin to seven behind hot three-point shooting.
But Crosby-Ironton coach David Galovich kept his team calm, calling timeouts to stop Breckenridge's momentum. His plan appeared to have its desired effect and No. 3 Crosby-Ironton (29-2) prevailed.
"Games this late in March are never going to be perfect," Galovich said. "[Breckenridge] applied great pressure, and there's no question their plan was to wear us down."
The Rangers' Trey Jacobs made 10 of 13 shots from the floor and led his team with 26 points. The Rangers shot 68.4 percent for the game, the Cowboys 30.6 percent.
Senior Noah Gindorff led the Rangers with 14 rebounds, including 10 in the first half. He also had 15 points and eight blocks.
"We were able to get in position, use our size and length," Gindorff said.
Stevin Lipp led Breckenridge (26-3) with 21 points and three steals.


text:
When you imagine a spy, do you have visions of James Bond skulking around some sexy Russian woman's bedroom? Maybe Jack Bauer saving the world day in and day out? How about the phrase "digital spy." Does it make you think of Wikileaks founder Julian Assange, or Edward Snowden, leaking classified government information? Would it surprise you to learn that the majority of …Read More »
Home/ Tag Archives: Digital spy