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NAHB Now
NAHB Now's Latest Posts
Labor Market Improvement Follows Housing Strength
Recent economic data have shown rapid improvement relative to dramatic declines in mid-spring. And housing has led the way.
NAHB and Hanley Wood Announce New Partnership
Hanley Wood/Meyers Research and NAHB today announced that BUILDER will cease to be the official magazine of NAHB at the end of 2020, as the two organizations prepare to launch a new home building data platform for NAHB members.
NAHB Closed to Commemorate Independence Day
NAHB's office is closed July 3 to commemorate our nation's birthday.
Message from NAHB Third Vice Chairman Candidate Carl Harris
Carl Harris, the candidate for the NAHB 2021 Third Vice Chairman, prepared a video address for the virtual Leadership Council meeting on June 19.
HBI Joins National Campaign to Promote Jobs in Energy-Efficiency Sectors
HBI joined a national campaign to raise awareness about career opportunities in the energy-efficiency sectors, with a focus on fostering a talent pipeline that is inclusive of low-income and other underserved populations.
Congress Extends PPP Through Aug. 8
The House Wednesday night unanimously approved an extension of the Paycheck Protection Program (PPP) through Aug. 8. The Senate voted Tuesday to extend the program, just hours before it expired at midnight.
IRS Provides Temporary Relief for LIHTC
In a victory for NAHB and its members, the Internal Revenue Service today announced temporary relief from certain requirements under Section 42 of the Internal Revenue Code for Low-Income Housing Tax Credit (LIHTC) projects. In light of financing and construction delays and other challenges resulting from COVID-19, NAHB requested in April that the IRS extend all relevant LIHTC deadlines.
NAHB Committees, Councils Strengthen BUILD-PAC's Coffers for November Elections
Throughout the week of NAHB's Spring Leadership Virtual Meetings, council and committee chairs reached out to their members to share the importance of investing in their businesses through BUILD-PAC. By the end of the week, 10 NAHB committees and council Board of Trustees achieved 100% participation in BUILD-PAC, NAHB's bipartisan political arm that helps elect pro-housing, pro-business candidates to federal office.
New Home Sales Jump in May as Housing Rebound Continues
In a further sign that the housing market is rebounding from the COVID-19 pandemic, sales of newly built, single-family homes rose 16.6% to a seasonally adjusted annual rate of 676,000 units in May from a downwardly revised reading in April, according to newly released data by the U.S. Department of Housing and Urban Development and the U.S. Census Bureau. The May rate is 12.7% higher than the May 2019 pace.
NAHB Announces New Chairman
At the conclusion of last Friday's Spring Leadership Meetings, NAHB Chairman Dean Mon informed the Board of Directors that he would be unable to complete the remainder of his term due to personal reasons and was resigning his chairmanship. Effective immediately, NAHB First Vice Chairman Chuck Fowke, a custom home builder from Tampa, Fla., will take on the duties of chairman.


text: We are asking you to help American Cancer Society keep up our fight against cancer and COVID-19. Join us to save lives, celebrate lives, and continue as a world health leader. We are reaching out to you to Share Our Appeal today YOU are the leaders that define American Cancer Society and our mission. Giving Tuesday Now!

subset: commoncrawl


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Spring Branch RV Park
RV ParkWrite a Review 10950 Hwy 281 N Spring Branch, TX 78070 830-855-2491 Official Website
Overview of Spring Branch RV Park
Last Price Paid: $44
Reported by JulMur on 6/25/2020
Longest RV Reported: 28 feet (Travel Trailer)
Reported by TWO PEAS AND THE POD on 2/29/2020
Elevation 1,049 ft / 319 m
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Reviews of Spring Branch RV Park 2 people have reviewed this location.
JulMur26' Grand Design Imagine XLS 22MLE
"Awesome place to stay! "
Reviewed 6/25/2020
|Nightly rate:
|Days stayed:
|Site Number:
Friendly, hospitable customer service with great communication up front and during stay. The RV park is very clean. Free Wifi works great and good speed! Great cell service as well. Convenient trash and recycle cans on site. Our site had Full Hookups with the option of 30 or 50 amp. Shady spot, spacious and very level site which made backing in much easier! Asphalt drive through campground and wide spots. Reservations can be made over email or phone. The cicadas were in the trees and "sang" us to sleep. It's right off the highway and we were 3 spots to the highway. Even then, the road noise was not terrible. The turn into the park is directly off of 281, so a good idea to slow down ahead of time! We will definitely stay here again when we are in town.
JulMur would stay here again
This review is the opinion of a Campendium member and not of Campendium.com
TWO PEAS AND THE PODFL 28' Airstream Tommy Bahama 27FBQ Ford F150 XLT - 3.5 EcoBoost - 6.5 ft bed
"Best private park we've ever been to!"
Reviewed 2/29/2020
|Nightly rate:
|Days stayed:
|Site Number:
We don't usually visit private parks, but we're glad we did this one!
We had a solar upgrade done to our trailer while visiting this park. The company we worked with, RV Solar Solutions, does several installs at this location every month in the winter season.
We also has a mobile RV/Boat detailer, Hill Country Shine, come and clean our trailer after spending 14 days on Padre Island National Seashore.
During our visit we received many Amazon packages and had our mail forwarded twice.
There are relatively new washer and dryers available. Complete with an air conditioned/heated lounge area with cable TV, an honor system beverage cooler and coffee, lending libraries for DVDs and paperback books.
The private bathrooms are just like you would find in someone's home, large clean showers with separate sinks and toilets.
The place is very pet friendly and you'll see many dogs here. They have a fenced in dog park where well behaved dogs can run off leash and socialize with the other canine residents.
There is a nice mix between over-nighters and extended stay visitors. There are also some really nice discounts for weekly, monthly and seasonal visitors.
All of these things I mention are not always allowed at most private parks, but here they really strive to please the customer. That's what happens when you stay at a family owned and operated park where they take pride in your experience while visiting.
My only ding is that it's located on a busy commuter road with lots of traffic, but at night time things quiet down quite a bit traffic wise.
TWO PEAS AND THE POD would stay here again
This review is the opinion of a Campendium member and not of Campendium.com
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Contact us to update this listing.
- 50 Amp
- 30 Amp
- 20 Amp
- water
- sewer
- Full Hookups
- wifi: yes
- basketball court
- playground
- rec hall
Site Amenities
- big rig
- pull thru
- dog run
- laundry
- propane
- restrooms: flush
Discount Clubs
- Military Discounts
- Senior Discounts
- dog run
Guadalupe River RV Park
We have mixed feelings about this RV Park/Campground
- Adventure Awaits
Guadalupe River State Park
Beautiful with well spaced sites
- goeb
Cranes Mill Park Campground
A Great Corp of Engineer Park located on Canyon Lake, TX
- Travels with Homer
Texas 281 RV Park
A shady, quiet park with good laundry facilities and nice residents.
- Davidmichael8657
Potters Creek Park
Great campground
- Herecomethecarpenters
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Live or cooked. The price of this item is subject to the market on the day, so please contact us here for today's price.
£ MARKET PRICE: this product is priced daily depending on the cost at market.
Please call the Farm Shop on 01475 568 989 to order.
Related products
Fencebay Kippers
£17.50 per kg
Fencebay Kippers are smoked in the traditional way using our locally caught herrings. They are not artificially dyed and retain
Squat Lobster Tails
£22.00 per kg
Please note - this product is seasonal and availability can vary, so please call us to check stock before ordering!
Hot Smoked Salmon Fillets
£32.00 per kg
Gently roasted in a smokey kiln our Hot Smoked Salmon Fillets come in a range of flavours including plain, cajun,


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Somfy Camera Wall Mount
- A discreet and elegant wall mount
- Rotating head enables viewing from many angles
- 2 screws + 2 pins for any surface
- 6.56" USB extension
Product Description
Somfy Camera Wall Mount
Somfy Camera Wall Mount
A discreet and elegant wall mount to hang your Somfy Security Camera up high, right on your wall.
A discreet and elegant wall mount to hang your Somfy Security Camera up high, for enhanced monitoring.
Rotating head: Though affixed to the wall, rotating head enables viewing from many angles and easy adjustment
Whats in the box
2 screws + 2 pins for any surface
6.56" USB extension
15 double-sided adhesives
Technical Specifications
Size / weight: 70 x 38,3 x 65 mm / 90 g
Included: 2 m flat USB extension cable, 15 transparent stickers for fixing cable to wall and 2 screws + 2 pins for any surface.
Requirements : Somfy Security Camera
Technical Specification
Somfy Camera Wall Mount
|Product Type
|Camera mount
|Placing / Mounting
|Wall mountable
|7 cm
|3.83 cm
|6.5 cm
|90 g
Product Reviews
Ask an Owner
Somfy Camera Wall Mount
Sorry, customer questions are not available for this product.
Product Discussion
Somfy Camera Wall Mount
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|Message view
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|Jeremy Dyer <jeremyd...@apache.org>
|Advice on connecting single NiFi instance to new cluster
|Sat, 14 Jul 2018 16:53:32 GMT
Team - I'm sitting up a small development environment. I have an existing NiFi instance that is pointed to a NiFi-Registry instance and its running several versioned flows. I wanted to join this instance with a cluster of 2 other nifi nodes. The 2 new nodes are fresh instances all running Nifi 1.7.0. However since the existing instance is already running flows it fails to join the new cluster because obviously the new nodes do not have anything running yet. What I really want is to be able to bootstrap those 2 new nodes with the same flows running on the existing instance but I can't seem to get that to work (boot fails and complains about the flows not being the same) however since I'm using the NiFi-Registry I'm not sure the best path forward? I realize that I could just wipe everything and start over but I wanted to try and solve the problem and maybe improve the documentation along the way to help others that might be having the same issue. Long story short what is the best way to get out of the pickle I'm in? Thanks, Jeremy Dyer
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Hitches and Towing
In live chat option, if the customer needs help, he can chat with a U-haul representative online. You need to press the live chat button, the pop-up will open and you need to enter the name to begin chat. After putting your name in the box click begin chat to get started. The representative will approach you as early as possible so please wait and you will be given the same directions. So chat…. In this article of Uhaul coupons, we shall discuss uhaul number. U-haul number is considered to be the toll free number. The caller instructions: Thank you for calling Uhaul Customer service phone number.
For trailer, truck, storage, moving and rental rates and information press 1. To know about the equipment drop off location press 2. For customer services regarding your existing or completed rental press 3. For emergencies help including road side accident or breakdown press 4. To give…. Uhaul Android App on Google Playstore Uhaul has designed this app to help you moving but it will not help you to pack your stuff.
You have to pack your stuff yourself. According to Uhaul, they wanted to make your moving easy, therefore, they have made this app for your convenience. We can also call Uhaul app as Uhaul moving and storage app. Uhaul mobile application is not only available on Google play store but also available on apple play store as well. Now you can book or rent Uhaul truck and trailer and also return them by using your Uhaul android app.
Additionally, you can also…. In U-Haul rental category, Uhaul van rental is also available.
You can rent a Uhaul cargo van at just Uhaul Van Rental When you rent a Uhaul cargo van then you can also get extended miles, week long and month long rentals too. Add your rating. Get a Free Month of Storage Get a month of free self-storage when you hire a one-way truck or trailer from participating locations. U-Haul Trucks have low decks and wide ramps for easy loading, gentle-ride Suspension ensures a smooth ride, fuel economy gauge helps you save money.
Get hour emergency road service, and your equipment reservation is guaranteed. Did this coupon work for you? Get Offer. Get one month free Self-Storage with one-way equipment rentals. Valid at U-Haul and participating Affiliate locations. Click through to see U-Haul's current promo codes, coupons, discounts, and special offers. You won't need a U-Haul coupon code for this discount! Guarantees free replacement including shipping and labor costs of the receiver hitch for any reason, with no limitation for any damage, including collision, corrosion, accidental overload, jackknifing and even theft of the vehicle.
Limited time offer.
Enter your location to see this deal! U-Box containers make moving and storage more convenient with less worry through various delivery and pick-up solutions which allows you all the time you need to load and unload your U-Box containers. So whether you are moving across town or across the world, storing, renovating, leaving school; U-Box containers have the right answer for all of your needs.
Discounts and Special Offers | U-Haul
UHaul can help because they have the right equipment at the lowest cost. Find the cheapest UHaul deals, coupons, discount codes, and to reserve your truck from here. You can save money on trucks, trailers, and other services using UHaul coupons and promo codes. UHaul with more than 60 years of service to North American on the move. It has been the do-it-yourself moving industry leader since They have over 15, convenient locations to save your time and money. You can reserve trucks, trailers, hitching accessories at lowest rates.
You can even get more discounts using money-saving discount codes and UHaul coupons.
35% off | 12 Budget Car Rental Coupons and Sales | PCWorld
Rent at any Nashville location and get one-month free self-storage unit. Show your receipt to get a discount. Online reservation use promo code. Storage access from Monday to Sunday: 5 am — 10 pm. Go and grab the deal.
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No discount code required. No UHaul discount code required. Checkout at UHaul to get the offer. No Coupon code needed.
U haul delivery
Reserve your U-Haul Truck or van for long distance at exclusive rates. It is also possible to get U-Haul business rental best deals and discounts. No coupon code needed. Uhaul offers the cheapest discount in the form of UHaul discount code and coupon often. They offer different discounts, giveaways, coupons and deals on many events and occasions.
Before you place an order, always visit UHaul website to get coupons and discount codes to save more. Always check coupys.
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Get all the latest discount codes and coupons from U-Haul Discounts and Specials. Uhaul often offers its customers a great discount, whenever they use UHaul discount code. But currently, they do not offer any type of military coupons and discounts. They already have a very affordable and competitive truck and trailer reservation rates. Packing up and moving has just become a whole lot easier. They have everything you need, to enter your house, store it or transport it.
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Their trucks have the lowest decks and the widest ramps which save you half the work.


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Once a card control function has been enabled or disabled, it will stay in that position until it is manually changed back.
Once you submit your application, we will endeavour to complete the application, including providing you with an answer within 48 hours business hours.
The answer that we provide you with will be pending the provision of a number of documents to confirm the details that you have provided in the application. At this stage the application simply depends on how quickly you are able to get these documents to us; once we have everything that we need, we are able to fund the credit card.
As this loan product can only be accessed with a card, we need to send a card out for you to use at this stage. Once we have funded the loan and ordered the card, it will take 5-10 business days to be sent to your nominated mailing address.
Once a card control function has been enabled or disabled, it will stay in that position until it is manually changed back.
Yes. Direct debits made against the card number will continue to be deducted.
If our Mobile Banking or Internet Banking service is not set up on your account, we won't be able to apply a temporary block. In this case, the card would need to be cancelled if it had been lost or stolen. If these services are not set up, then we would be happy to assist you with setting them up.
No. Once you make a change on one of your devices, the block will apply to your account which will be reflected across all your devices.
Yes. Cancelling your card will override any temporary blocks applied.


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With ties in the balance, EU and China hold tense summit
Chinese leaders and senior EU officials hold a video summit Monday with tensions rising over new tariffs targeting Chinese firms and European disquiet at Beijing's assertiveness on the world stage.
The heads of the EU's main institutions will discuss the coronavirus with top Chinese leaders, after Brussels infuriated Beijing by accusing it of a campaign of disinformation around the pandemic.
The EU will be represented by European Council President Charles Michel and European Commission head Ursula von der Leyen.
Premier Li Keqiang will be the main Chinese representative and President Xi Jinping is also expected to make a video statement.
China and the EU both say they want to strengthen ties, but the relationship is tangled in a thicket of disagreements on topics ranging from trade and investment rules to human rights and national security.
Beijing's ambassador to the EU said last week that Monday's talks were a chance to make relations "more productive and substantive by seizing opportunities and addressing challenges".
But he rapped Brussels over plans for tougher rules for heavily subsidised foreign companies, which would particularly target Chinese firms, saying the EU was not practising the openness it preached to others.
US President Donald Trump has pursued a bruising trade war with China, and warned last week of "complete decoupling" while suggesting the novel coronavirus is sometimes called "Kung Flu", in a jibe at China.
The EU has taken a more balanced approach, characterising China as a "systemic rival" and competitor but also as a partner on some issues. The bloc's foreign policy chief Josep Borrell has urged a hard-headed approach.
At talks with US Secretary of State Mike Pompeo last week, Borrell proposed a dialogue with Washington aimed at forging a common trans-Atlantic front against Beijing -- though he has also stressed the EU must pursue its own interests and not simply toe the American line.
Human rights
The bloc has sought to challenge China on human rights issues such as Tibet and Hong Kong, where European capitals are extremely concerned about Beijing's proposed new security law.
On Friday, the EU demanded the immediate release of a Chinese human rights lawyer jailed for "inciting subversion of state power" after penning an open letter calling for constitutional reforms.
But while such calls have occasionally irritated Beijing, they have done nothing to alter China's course and it is not expected to budge on Monday.
Cui Hongjian of the China Institute of International Studies said officials would defend their position on Hong Kong and explain the need for the new law -- as Foreign Minister Wang Yi did in recent talks with EU counterparts.
The EU's efforts to stand up to President Xi's increasingly assertive China have been stymied by a lack of unity among its 27 member states, many of which have been courted assiduously by the Asian giant.
A meeting of EU foreign ministers last month dedicated to China highlighted the bloc's struggles -- Borrell voiced "grave concern" at Beijing's security clampdown in Hong Kong but said sanctions were all but out of the question.
Trade and investment
The European Commission, the bloc's executive arm, has announced plans for new legislation aimed at ensuring foreign firms backed by heavy state subsidies do not distort competition in Europe.
There is particular concern about Chinese companies swooping in to buy European companies weakened by the coronavirus-triggered recession.
Specifically targeting China's global reach, the EU on Monday last week imposed an unprecedented tariff on Chinese subsidies to exporters, but not those in China itself.
The measure involved EU imports from Egypt of glass-fibre fabrics that were made by subsidiaries of two Chinese firms.
This was the first time the EU took aim at subsidies granted by a country to exporters located in another state.
Meanwhile, talks towards a China-EU investment agreement remain at a standstill, despite a joint pledge to try to get somewhere by the end of the year during the German EU presidency.
Video: EU and China hold tense video summit amid widening differences (AFP)
Russia warns of 'uncontrollable escalation' between US, IranRussian ambassador to the United Nations Vasily Nebenzya warns of an "uncontrollable escalation" as the United States presses to extend an arms embargo on Iran or impose UN sanctions that could kill a nuclear deal. SOUNDBITEAFP
Russia warns of 'uncontrollable escalation' between US, IranRussian ambassador to the United Nations Vasily Nebenzia warns of an "uncontrollable escalation" as the United States presses to extend an arms embargo on Iran or impose UN sanctions that could kill a nuclear deal. SOUNDBITEAFP
Iran FM says ending UN arms ban 'inseparable' from nuclear dealIran says that the preservation of a nuclear accord depends on the scheduled end in October of a UN arms embargo as the United States seeks to extend it. "The timetable for the removal of arms restrictions embodied in Resolution 2231 is an inseparable part of the hard-won compromise," Iranian Foreign Minister Mohammad Javad Zarif tells a Security Council session. SOUNDBITEAFP
EU and China hold tense video summit amid widening differences
Russia warns of 'uncontrollable escalation' between US, Iran
Russia warns of 'uncontrollable escalation' between US, Iran
Iran FM says ending UN arms ban 'inseparable' from nuclear deal
China says US, having left Iran accord, cannot trigger UN sanctions
US could see 100,000 new coronavirus cases a day: Fauci
Syria donors conference pledges 6.9 billion euros
Dr Anthony Fauci arrives on Capitol Hill for Senate hearing
Macron arrives in Mauritania for Sahel anti-jihad summit
China's HK security law will only target 'small minority': Lam
EU 'deplores' China's Hong Kong security law
On board the Ocean Viking, tension mounts among rescued migrants
UK reimposes lockdown on virus-hit city of Leicester
Salvini is heckled during visit to virus-hit 'ghetto' in southern Italy
After six months, pandemic 'not even close' to being over: WHO
French ex-PM Fillon and wife arrive for Paris court's decision


text: Member Directory Rep Roster New SearchPrev1Next Ms. Tricia EbertRealtorThe Ebert-Sheesley Team The Ebert-Sheesley TeamTricia Ebert & Ernie Sheesley, RealtorsCalBRE #01787058 & 01973830 eXp Realty of California, Inc.Tricia Ebert has been a Realtor since 2006, serving Los Angeles County and specializing in the San Fernando Valley and Santa Clarita Valley areas. Ernie Sheesley has been licensed since 2015 and joined Tricia in serving her clients, creating The Ebert-Sheesley Team. Tricia has been a resident of both Burbank (for 20 years) and Santa Clarita (for 10 years). Ernie has been a resident of Burbank for over 30 years.We have helped many people find and buy their dream homes as well as sell their homes when the market was very challenging. Our goal as your Realtor is to take away all the stress of buying or selling a home. We handle the massive amount of paperwork involved and negotiate the best terms for everyone concerned. You can read the Testimonials from our happy clients on our website: www.EbertSheesleyTeam.com/TestimonialsRemember, "Don't Make a Move Without Us!" 818-679-3191E-mail New SearchPrev1Next Member Directory | Calendar of Events | Member Account | Community Calendar of Events | Jobs | Member Promotions | News | Request Info Upcoming Events No upcoming events. Check back soon. New Members Victory Auto Body & paintGlobal Wave IntegrationHappier Home CareMagnolia Park Merchants AssociationBottle Coatings Inc. creator of SanitisprayMaintco Corp.Hands to Heart, Inc.Interior Design Firm: A Psychotherapy PracticeThe Osbrink AgencyCanyon Road FilmsIvy Avanessian Fua, DDS, INCAssembly Dude Inc.Lorraine Richardson: Lorraine's CreationsD-Light Me Holistic Health CoachingPaylocity


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MILWAUKEE (WITI) -- Those who are thinking of booking a spring break getaway for the whole family need to act fast! Time is running out to book flights and hotels -- especially for those hoping to snag a deal!
Easter is early this year, which means less time to book that spring break trip.
"This more compact travel season with this high demand, we typically see that the hotel rooms and the airfare seats book out sooner," CNN Orbitz Expert Jeanenne Tornatore said.
According to Orbitz, the number one spring break destination for families is Orlando, Florida -- home of Walt Disney World.
For those not looking to spend that kind of cash -- the Wisconsin Dells is another good option.
FOX6's Contact 6 found booking a room with water passes at Kalahari, Wilderness or Great Wolf Lodge room rates all about $100 cheaper the week after Easter Sunday -- compared to the week before.
Blue Harbor Resort in Sheboygan offers a $19.99 water park day rate. Country Springs Water Park in Pewaukee offers a $25 water park day rate.
With the tail end of winter weather sticking around, FOX6's Contact 6 says travelers should prepare for weather disruptions.
"Even if you're planning on heading to somewhere like a great warm weather destination, you want to prepare yourself for any connections that may cause you trouble and make sure that you have anything you really need for 24 hours packed with you in your carry-on bag," Tornatore said.
If the airline does lose a bag, there's a max payment of $3,300 bucks -- so don't pack expensive camera or computer gear. If you get bumped from a flight, and the airline reroutes you and you're more than two hours late, you can be compensated up to four times the value of your ticket.
The Department of Transportation requires airlines to provide information on how to file a complaint and the company must send a response in 60 days.


text: Categories Miscellaneous 2012 Best Days for Holiday Gift Discounts Post author By MrWalker Post date November 16, 2012 No Comments on 2012 Best Days for Holiday Gift Discounts Share this:Click to share on Twitter (Opens in new window) Related posts: littleBits: Best of Toy Fair 2012 Maker Faire 2012 Google Zeitgeist 2012 Modern Warfare 3 Passes $1 Billion in Sales in 16 Days Tags Infographic ← VPNs on the Rise → Sheldon's T-Shirts of the Big Bang Theory Leave a Reply Cancel replyYour email address will not be published. Required fields are marked *Comment Name * Email * Website Notify me of follow-up comments by email. Notify me of new posts by email.


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Greek Athenian Soldier Helmet Pewter Pin Pinback Badge Tie Tack 1HPN# 10055
If you compete in Greek military strategy games or role playing fantasy competitions, you'll want to wear this Greek Helmet Athenian Military pin on your tie, cap or backpack to show your spirit. Also, you can teach children about the history of ancient Greece. The helmet has a large plume and face mask.
- Greek Helmet pin is made from lead free metal with secure pin clasp. It is half a helmet in a profile view. PN 10055.
- Measures 7/8 in H x 1 1/8 in W x 1/4 in D.
The themes and creatures from Greek mythology were common on coins and jewelry in ancient Greece. Jewelry made from gold and precious stones would have only been worn by the wealthy, normally for special occasions.
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Garden of Earthly Delights Triptych by Bosch, Three Panel Folding Card 5.5HPN#JBC01


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Despite popular myths about the unpleasantness of root canal therapy, state-of-the-art techniques and proper anesthesia can make the procedure virtually painless. Find out what's involved in having this pain-relieving treatment to save your tooth.
Tooth brushing is a critical component of good oral health. But your dental care routine shouldn't stop there. To be truly effective, your routine needs to include flossing.
In fact, some evidence suggests flossing is responsible for as much as 40 percent of plaque removal, which is essential for oral health. ("Plaque" is the name for the soft, sticky film of bacteria that naturally accumulates on our teeth as a result of eating and digestive processes. When we skip brushing and/or flossing, this plaque hardens into tartar, which is much more difficult to remove.)
Dentists can stress the importance of flossing until we're blue in the face (and we do!), but it's up to patients to make flossing a priority. If you've ever skipped flossing or wondered why flossing is such a big deal, we have six good reasons why you should commit to the habit for life. Here's what happens when you neglect to floss your teeth.
Plaque is one of the biggest risk factors for cavities. It doesn't just form on the teeth's biggest surfaces; it also congregates between the teeth. So do food particles, which also contribute to tooth decay if left to sit between your teeth. Even the best toothbrush can't reach every one of your teeth's crevasses, but dental floss can. Thus, flossing allows you to target the plaque and food particles that would otherwise stay wedged between the teeth and potentially cause cavities to form.
Flossing doesn't just remove plaque from between the teeth. It's also the most effective way to eliminate plaque and bacteria near the gum line. When this bacteria is allowed to accumulate, it significantly increases the risk of gum diseases including gingivitis and periodontitis (which, if left unchecked, can contribute to tooth loss).
It might sound implausible, but there's a proven link between gum disease and cardiovascular disease. This is largely because the gums contain loads of tiny blood vessels. When bacteria builds up along the gum line, it can enter these blood vessels and travel through the body's bloodstream. The presence of this bacteria in the bloodstream has the potential to harden the arteries of the heart, thereby increasing the risk of a heart attack or stroke.
Keeping the areas between your teeth clean helps ensure the whole tooth surface looks to be the same shade. This makes your entire set of chompers look whiter overall. In contrast, when you allow plaque and food particles to accumulate between the teeth, this darkens the shade at the edges of your teeth and makes your teeth appear yellower.
As we mentioned above, when you don't floss, you allow tiny food particles to accumulate between your teeth. As these bits of food decay, they produce unpleasant odors. This is a major contributor to halitosis (aka bad breath).
Skipping one flossing session might seem relatively harmless. And it is, in the grand scheme of things. The trouble is that when we allow ourselves to skip flossing one time, it becomes that much easier to do it a second time (and a third, and a fourth, and so on...). It's better to maintain a consistent habit than to give yourself the opportunity to slack on flossing.
So there you have it: six very good reasons why you should never skip flossing again. If you aren't a habitual flosser, recommit to a daily flossing routine right now. Your mouth (and the rest of your body) will thank you for years to come.
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Diana Quiseekay (born 28th June 1932 in Liverpool, died 1981 in London) had two separate roles on Coronation Street: Janice in September 1967; and she returned to the programme in October 1975 as Sophie Edwards, a barmaid at the Gatsby Club.
Born and brought up in Liverpool, she left Merseyside at the age of sixteen to join an American stage company as a dancer and sang around a large part of the United States and various other regions of the world. In 1965 she appeared in the West End musical Maggie May and was later a resident singer at London's Talk of the Town.
Her other credits include Z Cars and Doctor In Charge.


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Current consultation, council submissions, Annual Residents Survey and official information requests
Minutes, agendas and how you can participate
Everything you need to know about building consents, PIMs and development contributions
Dogs, Stock, Poultry and Bees
Arts and culture in Ashburton District
Learn about our district's economy
Thursday 26 September 2019
Once every three years, an important occasion rolls around for communities across the country that determines the priorities, decisions, and outcomes that will affect local people. I'm speaking, of course of the local government elections, and now is that all-important time to decide who you would like to represent you around the Ashburton District Council table.
Voting is now open for elections until 12 noon, Saturday 12 October. If you are an enrolled voter, your ballot papers should have arrived in your mailbox in the last few days, as they began distribution on 20 September.Your voting papers are powerful documents. They are your opportunity to influence local democracy and make your voice heard. They are your chance to consider what's important to you and select officials that you believe will most effectively represent those views.For our district, elections are being held for the mayoralty; the Ashburton, Western and Eastern Wards; and the Ashburton Licensing Trust. Four nominations were received for five vacancies to the Methven Community Board, and therefore these candidates have been declared elected. A by-election will be called for the remaining vacancy later this year.
In addition to the Council, you are also invited to elect representatives for Environment Canterbury and the Canterbury District Health Board.
Local governments make decisions that affect infrastructure, services, and activities that you use and access every day. It's the water coming out of your taps, the roads you drive on, the community halls and facilitates you go to, the funding your community group applies for, and the attraction of investment and business to town that creates jobs and supports economic growth.
Being able to choose who makes these decisions on your behalf is an opportunity I strongly urge all voters to take up.This chance only comes around once every three years, so be sure to make your voice heard this election.Whether you are young, old, working, retired, studying, have a family, are independent, have lived here all your life or have not long come to the Ashburton District, the local government elections are for you.
Don't neglect the special envelop that might be sitting on your kitchen table. Fill out the ballot papers, put them into the pre-paid envelop and drop them into the post no later than Tuesday 8 October, or alternatively, take them to the Council office by 12 noon, 12 October.


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Thursday, May 8, 2014
On Socialist Madman/Murderous Thug, Robert Mugabe, Embracing the Hitler Analogy
Not the strategy that I would have recommended, absent a bazooka aimed at my forehead.
Posted by Will "take no prisoners" Hart at 7:30 PM
2 comments:
- dmarks said...
He is a very typical socialist leader.
- May 9, 2014 at 6:07 PM
- Will "take no prisoners" Hart said...
He's amongst the worst of the worst. He's taken a country that was at one point one of the breadbaskets of the world and turned it into a veritable hell-hole. Compare THAT to Botswana which has largely embraced free markets and the comparison is even more pronounced.......The only thing left here is for wd to quote the bastard.
- May 9, 2014 at 9:07 PM
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The genotoxicity of cationic lipopeptide nanoparticles (cLPNPs) was evaluated in vivo and in vitro comet assay and the in vivo chromosome aberrations test. In vitro comet assay, human blood cells were exposed to cLPNPs at the concentration of 2.5, 5, 10, 20, 40 and 100 µg/mL. Significant DNA damage was observed after 1 h exposure, but no effects were detected after 3 h. In vivo, cLPNPs were administered in single or five daily injection doses at 8, 20 and 40 mg/kg of body weight by subcutaneous injection to male mice. The cLPNPs caused DNA damage in the liver, lung and kidney, but not in the spleen. The kidney was more prone to genotoxic effects that persisted from 24 h to 14d after a single injection of cLPNPs. No statistically significant increase in the percentage of cells with chromosomal aberrations above the vehicle control was observed in mice bone marrow after a single or repeated injection of cLPNPs. In summary, cLPNPs shown to be genotoxic both in vivo and in vitro. The results suggest the importance of the use of highly sensitive methods, such as the comet assay, in order to determine the full genotoxic potential of nanoparticles.
AssuntosAberrações Cromossômicas/induzido quimicamente , Dano ao DNA , Lipopeptídeos/toxicidade , Nanopartículas/toxicidade , Animais , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/patologia , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Relação Dose-Resposta a Droga , Humanos , Injeções Subcutâneas , Rim/efeitos dos fármacos , Rim/patologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/patologia , Lipopeptídeos/química , Fígado/efeitos dos fármacos , Fígado/patologia , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Nanopartículas/química
Cardiac catheterization procedures are performed on about 20,000 children with congenital heart disease (CHD) annually in China. The procedure, which involves exposure to ionizing radiation, causes DNA damage and may lead to increased cancer risk. We have studied chromosomal aberrations (CA) in peripheral lymphocytes of CHD children. CA frequencies were assessed in an interventional group of 70 children who underwent cardiac catheterization and a control group of 51 children receiving open-heart surgery. Total CA and all chromosome-type aberrations were higher in the exposed children than in the control group. With respect to the type of septal defect, the translocation frequency was higher in patients with ventricular rather than atrial defects. Cardiac catheterization procedures increase CA frequencies and may also increase the risk of cancer.
AssuntosCateterismo Cardíaco/efeitos adversos , Aberrações Cromossômicas/efeitos da radiação , Cardiopatias Congênitas/cirurgia , Linfócitos/efeitos da radiação , Radiação Ionizante , Adolescente , Cateterismo Cardíaco/métodos , Estudos de Casos e Controles , Criança , Pré-Escolar , China , Feminino , Cardiopatias Congênitas/patologia , Humanos , Linfócitos/imunologia , Masculino , Duração da Cirurgia , Cultura Primária de Células , Risco
BACKGROUND: This study aimed to investigate the incidence and clinical significance of segmental chromosomal aberrations (SCAs) in Korean patients with neuroblastoma. METHODS: Patients diagnosed with neuroblastoma from 2012 to 2018 were included for retrospective review. Fluorescence in situ hybridization (FISH) was used to analyze four SCAs (MYCN amplification, 1p deletion, 11q deletion, and 17q gain). Clinical characteristics at diagnosis, early tumor response (reduction in primary tumor volume and neuron-specific enolase level after the first three cycles of chemotherapy), and survival rates were compared according to SCAs. RESULTS: Among 173 patients with FISH results, 92 (53.2%) had at least one of the four SCAs, while 25 (14.5%) had two co-aberrations, and eight (4.6%) had three co-aberrations. SCAs detected in our study were MYCN amplification (n = 17, 9.8%), 1p deletion (n = 26, 15.2%), 11q deletion (n = 44, 25.6%), and 17q gain (n = 46, 27.1%). Patients with MYCN amplification showed a better early response but a worse survival than those without (5-year overall survival: 46.2% ± 13.1% vs. 88.6% ± 3.4%). Furthermore, 1p deletion was associated with a better early response but a worse survival; however, it was not an independent factor for survival. We could not find any prognostic significance associated with 11q deletion or 17q gain. CONCLUSION: This is the first study investigating SCAs in Korean neuroblastoma patients. Prognostic significance of SCAs other than MYCN amplification was different from those reported in western countries. Further study with a larger cohort and longer follow-up is needed to confirm our findings.
AssuntosAberrações Cromossômicas , Neuroblastoma/genética , Adolescente , Criança , Pré-Escolar , Cromossomos Humanos , Feminino , Amplificação de Genes , Humanos , Hibridização in Situ Fluorescente , Lactente , Recém-Nascido , Masculino , República da Coreia , Estudos Retrospectivos , Adulto Jovem
5-Fluorouracil (5-FU) is a widely used antineoplastic drug. In this work, a comprehensive study was performed to detect the extent of chromosomal damage and morphological sperm defects induced by 5-FU in male mice and the possible protective role of the iridoids-rich fraction of Pentas lanceolata leaves (IFPL). Six main groups were examined in micronucleus and chromosomal assays: I- control negative, II- control positive (i.p. treated with single dose of 75 mg/kg 5-FU), III- control plant (orally administrated IFPL, 300 mg/kg, 5 consecutive days), and IV-VI- treated with IFPL (100, 200 and 300 mg/kg, 5 consecutive days) plus 5-FU (i.p. treated at the last day). Samples were taken 24 h post treatment. The study of morphological sperm anomalies, single and repeated treatments were examined and samples were taken after 35 days from the 1st treatment. In bone marrow, 5-FU induced a significant increase in the micro-nucleated polychromatic erythrocytes, chromosome anomalies (CAs) and also cytotoxic effects. A significant percentage of CAs was recorded in spermatocytes after 5-FU treatment reached 22.80 ± 1.32 vs 4.20 ± 0.37 for control (mainly X-Y univalent, 90%). IFPL was recorded to be non-mutagenic in all tests examined. In addition, it alleviated the previous defects in a dose-dependent manner. A significant and dramatic increase in the percentage of morphological sperm defects was recorded after single and repeated treatments with 5-FU reached 13.24 ± 0.24, 30.42 ± 0.32 respectively vs 2.56 ± 0.14 for control. Amorphous head-sperm and sperm with coiled tail were the most pronounced types of abnormalities. Significant protection was detected with the highest tested dose of IFPL. In conclusion: 5-FU demonstrated to be a genotoxic agent. Its genotoxicity in germ cells is serious and may lead to reproductive toxicity, infertility or heritable defects. The results also demonstrated the biosafety of IFPL and its possible protective role in combined treatment with 5-FU.
AssuntosIridoides/farmacologia , Extratos Vegetais/farmacologia , Rubiaceae/química , Espermatozoides/efeitos dos fármacos , Animais , Aberrações Cromossômicas/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Fluoruracila/efeitos adversos , Fluoruracila/uso terapêutico , Iridoides/química , Masculino , Camundongos , Testes de Mutagenicidade , Extratos Vegetais/química , Espermatócitos/efeitos dos fármacos , Espermatócitos/patologia , Espermatozoides/patologia
The aim of the present study was to assess the oxidative stress level and chromosomal damage induced by occupational exposure to low dose ionizing radiation (LDIR). Two hundred and eighteen hospital workers occupationally exposed to LDIR were included in this study, along with 118 healthy age- and gender-comparable controls. Occupational dosimetry records were collected over the last year and revealed that the accumulated annual dose for each hospital worker was below the permissible limit of the International Commission on Radiological Protection (ICRP). The individuals' oxidative and antioxidative status were determined by measuring the activities of copper zinc-superoxide dismutase (CuZn-SOD), glutathione peroxidase (GSH-Px), catalase (CAT) enzymes, and the levels of malondialdehyde (MDA) in erythrocytes. The effect of radiation on chromosomal integrity was measured by the frequency of micronuclei (MN) formation using the cytokinesis block technique. Our results showed that the activities of CuZn-SOD and CAT enzymes and MDA levels observed in the hospital workers were higher than those in the controls (p < 0.05). We did not find significant difference in GSH-Px enzyme activity between the two groups (p = 0.247). A higher frequency of MN was found in exposed groups than in the controls [3(1-5) versus 2(0.75-4) ; p<0.001]. The difference was significant for males (p = 0.012), but not females (p = 0.14). Multiple linear regression analysis showed differences in the oxidant activities and MN frequency between hospital workers and controls adjusted for age, gender, smoking status and drinking status. Correlation analysis indicated that the frequency of MN was positively associated with MDA levels (p < 0.05). Altogether, these results support the detrimental effects of chronic low dose radiation in humans, which involves the induction of oxidative stress and chromosomal damage.
AssuntosAntioxidantes/metabolismo , Aberrações Cromossômicas/efeitos da radiação , Exposição Ocupacional/efeitos adversos , Estresse Oxidativo/efeitos da radiação , Adulto , Antioxidantes/efeitos da radiação , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Hospitais , Humanos , Masculino , Malondialdeído/sangue , Registros Médicos , Pessoa de Meia-Idade , Doses de Radiação , Radiação Ionizante , Radiometria , Superóxido Dismutase-1/sangue
RATIONALE: Co-occurrence of cytogenetic and molecular abnormalities is frequently seen in patients with acute myeloid leukemia (AML). The clinical outcome and genetic abnormalities of AML may vary; therefore, genetic investigation must be complex, using several techniques, to have an appropriate characterization of the AML genome and its clinical impact. The available molecular markers can predict prognosis only partially. Acute promyelocytic leukemia subtype M3 (AML M3) is a subtype of AML characterized by the presence of promyelocytic leukemia-retinoic acid receptor alpha (PML-RARA) genes fusion. Targeted treatment with all-trans-retinoic acid (ATRA) and ATRA combined with arsenic trioxide significantly improved the survival of AML M3 patients. Unknown prognostic factors could contribute to the early death of these patients. PATIENT CONCERNS: We present the case of a young female (20 years old) patient, who presented at the emergency department 5 months after giving birth to her first child, complaining of asthenia, fatigue, general musculoskeletal pain, and fever (38°C), symptoms having been present for the previous 6 days. The patient denied any chronic diseases in her medical and family history. DIAGNOSIS: Laboratory analysis revealed severe pancytopenia. Cytogenetic and molecular analyzes revealed chromosomal abnormalities (trisomy 8), PML-RARA gene fusion, and fms-like tyrosine kinase 3 (FLT3) gene mutation. The immunophenotypic analysis was also suggestive for AML M3 according to the FAB classification. INTERVENTIONS: Specific treatment was initiated for AML M3 and for secondary conditions. Molecular and cytogenetic analyzes were performed to have a more detailed characterization of the patient's genome. OUTCOME: Seventy-two hours after admission, she developed psychomotor agitation, confusion, coma, and convulsion. Subsequent deterioration and early death were caused by intracerebral hemorrhage with multiple localization and diffuse cerebral edema. LESSONS: The presence of FLT3 internal tandem duplication (ITD) mutation may explain the rapid and progressive degradation of this AML M3 case and it may be used as a prognostic marker even when co-occuring with other markers such as PML-RARA gene fusion and trisomy 8. We consider that FLT3 ITD mutation analysis in young patients with AML should be performed as soon as possible. New strategies for patients' education, AML (or cancers in general) prevention, and treatment are needed.
AssuntosFusão Gênica/genética , Leucemia Mieloide Aguda/genética , Proteína da Leucemia Promielocítica/genética , Receptor alfa de Ácido Retinoico/genética , Trissomia/genética , Tirosina Quinase 3 Semelhante a fms/genética , Aberrações Cromossômicas , Cromossomos Humanos Par 8/genética , Evolução Fatal , Feminino , Humanos , Mutação , Sequências de Repetição em Tandem , Adulto Jovem
Retroperitoneal liposarcomas are rare tumours that carry a poorer prognosis than their extremity counterparts. Within their subtypes - well differentiated (WDL), dedifferentiated (DDL), myxoid (MLS) and pleomorphic (PLS) - they exhibit a diverse genomic landscape. With recent advances in next generation sequencing, the number of studies exploring this have greatly increased. The recent literature has deepened our understanding of the hallmark MDM2/CDK4 amplification in WDL/DDL and addressed concerns about toxicity and resistance when targeting this. The FUS-DDIT3 fusion gene remains the primary focus of interest in MLS with additional potential targets described. Whole genome sequencing has driven identification of novel genes and pathways implicated in WDL/DDL outside of the classic 12q13-15 amplicon. Due to their rarity; anatomical location and histologic subtype are infrequently mentioned when reporting the results of these studies. Reports can include non-adipogenic or extremity tumours, making it difficult to draw specific retroperitoneal conclusions. This narrative review aims to provide a summary of retroperitoneal liposarcoma genomics and the implications for therapeutic targeting.
AssuntosLipossarcoma/genética , Neoplasias Retroperitoneais/genética , Animais , Antineoplásicos Alquilantes/uso terapêutico , Aberrações Cromossômicas , Genômica/métodos , Humanos , Lipossarcoma/tratamento farmacológico , Lipossarcoma/metabolismo , Proteínas de Fusão Oncogênica/genética , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Neoplasias Retroperitoneais/tratamento farmacológico , Neoplasias Retroperitoneais/metabolismo , Trabectedina/uso terapêutico
OBJECTIVE: To explore the value of CpG-oligonucleotide(CpG-ODN) immunostimulatory method in chromosome culture of chronic lymphocytic leukemia (CLL) cells and to compare the differences between related studies at home and abroad, so as to improve the success rate of CLL karyotype culture and the detection rate of abnormal karyo-types. METHODS: Bone marrow samples from 82 CLL patients were collected and cultured with phytohemagglutinin (PHA), CpG-oligonucleotide plus interleukin-2 (CpG-ODN DSP30+IL-2) for 72 hours. Chromosomes were prepared and analyzed by conventional cytogenetics (CC). Meanwhile, D13S25, Rb1, ATM, p53 and CSP12 probes were used for interphase fluorescence in situ hybridization (iFISH) test. The differences of chromosome culture and iFISH test results between two cell stimulants were compared. RESULTS: The success rate of karyotype culture in PHA and CpG-ODN DSP30+IL-2 immunostimuli (analyzable mitotic t >20) was 90.2% (74 cases), 68.3% (56 cases) respectively, and the detection rate of abnormal karyotype was 13.5% (10 cases) and 46.4% (26 cases), respectively. The success rate of karyotype culture in PHA group was significantly higher than that in CpG-ODN DSP30+IL-2 group (P=0.01). The detection rate of abnormal karyotypes in CpG-ODN DSP30+IL-2 group was significantly higher than that in PHA group, and the difference was statistically significant (P=0.003). The detection rate of abnormal karyotypes in iFISH group was 74.4% (61 cases), which was significantly higher than that in CpG-ODN DSP30+IL-2 group (P=0.000). iFISH detection could verify the abnormalities detected by CC analysis. CONCLUSION: Application of CpG-ODN DSP30+IL-2 immunostimulation method in culture of CLL cells can enhance the detection rate of abnormal karyotypes, especially the detection of various translocations suggesting poor prognosis.
AssuntosLeucemia Linfocítica Crônica de Células B , Aberrações Cromossômicas , Humanos , Imunização , Hibridização in Situ Fluorescente , Oligodesoxirribonucleotídeos
OBJECTIVE: Chromosome detection is important in the diagnosis and prognosis of Myelodysplastic syndrome (MDS) patients. About 50% of MDS patients have chromosomal abnormalities. Moreover, chromosome 5 and 7 are common genetic abnormalities in MDS patients and use to identify prognosis risk group and the proper treatment in MDS patients. The objective of this study was to evaluate chromosomal abnormalities and clinical features of MDS patients in upper northern Thailand. METHODS: Fifty bone marrow (BM) specimens were examined by conventional cytogenetic (CC) technique and fluorescence in situ hybridization (FISH) technique for detected chromosome 5 and 7 abnormalities. The clinical features were comparison between MDS patients with chromosomal abnormalities and those with normal karyotype. RESULTS: Chromosomal abnormalities were detected in 8/50 MDS patients by CC and 17/50 cases by FISH technique. When the CC and FISH techniques were combined, chromosomal abnormalities increased to 21/50 cases. Abnormalities of isolated chromosome 5 were found in 13 cases and were associated with lower level of percentage blast of BM (p = 0.003) and higher level of hemoglobin (p = 0.019). Moreover, abnormalities of chromosome 7 were found in 3 cases, 1 case of isolated del(7q) and 2 cases of -7 and del(7q) with complex abnormalities. These three cases were associated with higher level of percentage blast of BM (p = 0.010). CONCLUSION: This study showed the frequency and pattern of chromosomal abnormalities of MDS patients in upper northern Thailand were different from other populations. MDS with isolated chromosome 5 abnormalities had clinical characteristics corresponding with patients in good prognosis risk group. However, MDS patients with chromosome 7 and complex abnormalities showed higher percentage blast of BM which high risk to progression to acute myeloid leukemia (AML). Combined CC and FISH techniques detect chromosomal abnormalities with greater frequency than when either technique is used alone.
AssuntosAberrações Cromossômicas , Síndromes Mielodisplásicas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Cromossomos Humanos Par 5 , Cromossomos Humanos Par 7 , Feminino , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Masculino , Pessoa de Meia-Idade , Tailândia
OBJECTIVE: To assess the value of chromosomal microarray analysis (CMA) for the analysis of 824 samples from miscarriage or stillbirth. METHODS: Copy number variations (CNVs) in the abortic chorionic villi or stillbirth tissues were detected by CMA. RESULTS: All specimens were successfully analyzed, among which 381 (46.2%) were diagnosed with chromosomal abnormalities, which included 312 (81.9%) numerical abnormalities, 66 (17.3%) structural abnormalities and 3 (0.8%) uniparental disomies. Among numerical chromosomal abnormalities, aneuploidies was most common (92.0%), with trisomy 16 and 45,X accounting for 41 (13.1%) and 63 (20.2%) of the cases, respectively. Among the 66 structural chromosomal aberrations, there were 26 (39.4%) CNVs duplications, 20 (30.3%) CNVs deletions, and 20 (30.3%) CNVs duplication and deletions. 33 CNVs were predicted as have a high chance to lead to a disease. CONCLUSION: CMA is a reliable, robust, and high-resolution method for the analysis of miscarriage or stillbirth samples. Numerical aberrations, in particular chromosomal aneuploides, are the main cause for spontaneous abortions and stillbirths.
AssuntosAborto Espontâneo/genética , Aberrações Cromossômicas , Transtornos Cromossômicos , Análise em Microsséries , Natimorto/genética , Transtornos Cromossômicos/diagnóstico , Transtornos Cromossômicos/genética , Variações do Número de Cópias de DNA , Feminino , Humanos , Gravidez
OBJECTIVE: To assess the value of combined chromosomal karyotyping and chromosomal microarray analysis (CMA) for prenatal diagnosis. METHODS: G-banding karyotyping and CMA were simultaneously performed on 546 women who were subjected to amniocentesis during middle pregnancy. RESULTS: In total 82 cases were detected with chromosomal abnormalities. The two methods were consistent in 43 cases, which included 14 trisomy 21, 6 trisomy 18, 1 trisomy 13, 14 sex chromosomal aneuploidies, 4 chromosomal deletions, 3 chromosomal duplications and 1 sex chromosomal mosaicism. Fifteen fetuses with chromosomal abnormalities detected by CMA were missed by karyotyping analysis, which included 9 microdeletions and 6 microduplications. Sixteen fetuses with chromosomal abnormalities detected by karyotyping analysis were missed by CMA, which included 15 chromosomal translocations and 1 sex chromosomal mosaicism. In 7 cases, the results of karyotyping analysis and CMA were inconsistent. One supernumerary marker chromosome detected by karyotyping analysis was verified by CMA as 9p13.1p21.1 duplication. CONCLUSION: Combined chromosomal karyotyping and CMA can significantly improve the detection rate for chromosomal abnormalities, which has a great value for prenatal diagnosis.
AssuntosTranstornos Cromossômicos , Cariotipagem , Análise em Microsséries , Diagnóstico Pré-Natal , Aberrações Cromossômicas , Transtornos Cromossômicos/diagnóstico , Transtornos Cromossômicos/genética , Feminino , Humanos , Gravidez
OBJECTIVE: To carry out genetic testing for 3 fetuses with abnormal prenatal screening. METHODS: Fetal ultrasound, karyotype analysis, single nucleotide polymorphism (SNP) array and fluorescence in situ hybridization were performed. RESULTS: Abnormalities of chromosome 22 were found with all 3 fetuses. Fetus 1 harbored a 7.1 Mb deletion in 22q13.2q13.33 region, which involved 54 OMIM genes including SHANK3 and FBLN1. Fetus 2 had a mosaicism karyotype, with 12% of cells harboring a 6.6 Mb deletion in 22q13.31q13.33, covering 48 OMIM genes such as SHANK3 and PPARA, and 5% of cells harboring a 26.1 Mb duplication in 22q11.1q13.2 involving 285 OMIM genes. Fetus 3 carried a tandem duplication of 1.7 Mb in 22q11.1q11.21, which involved 10 OMIM genes including CECR1, CECR2 and ATP6V1E1. No abnormality was found in the three couples by chromosomal karyotyping and SNP array analysis. CONCLUSION: The severity of diseases caused by chromosome 22 abnormalities not only depends on the range of the deletion or duplication, but is also closely related to chromosome structure, gene dose and genetic environment. Combined ultrasonography and various genetic testing techniques in prenatal diagnosis can greatly increase the detection rate of genetic diseases with substantial phenotypic variation.
AssuntosTranstornos Cromossômicos/diagnóstico , Cromossomos Humanos Par 22/genética , Testes Genéticos , Cariotipagem , Diagnóstico Pré-Natal , Ultrassonografia Pré-Natal , Aberrações Cromossômicas , Deleção Cromossômica , Transtornos Cromossômicos/genética , Feminino , Feto , Humanos , Hibridização in Situ Fluorescente , Gravidez , Fatores de Transcrição
Despite next-generation sequencing, which now allows for the accurate detection of segmental aneuploidies from in vitro fertilization embryo biopsies, the origin and characteristics of these aneuploidies are still relatively unknown. Using a multifocal biopsy approach (four trophectoderms [TEs] and one inner cell mass [ICM] analyzed per blastocyst; n = 390), we determine the origin of the aneuploidy and the diagnostic predictive value of segmental aneuploidy detection in TE biopsies toward the ICM's chromosomal constitution. Contrary to the prevalent meiotic origin of whole-chromosome aneuploidies, we show that sub-chromosomal abnormalities in human blastocysts arise from mitotic errors in around 70% of cases. As a consequence, the positive-predictive value toward ICM configuration was significantly lower for segmental as compared to whole-chromosome aneuploidies (70.8% versus 97.18%, respectively). In order to enhance the clinical utility of reporting segmental findings in clinical TE biopsies, we have developed and clinically verified a risk stratification model based on a second TE biopsy confirmation and segmental length; this model can significantly improve the prediction of aneuploidy risk in the ICM in over 86% of clinical cases enrolled. In conclusion, we provide evidence of the predominant mitotic origin of segmental aneuploidies in preimplantation embryos and develop a risk stratification model that can help post-test genetic counseling and that facilitates the decision-making process on clinical utilization of these embryos.
AssuntosBlastocisto/fisiologia , Embrião de Mamíferos/fisiologia , Desenvolvimento Embrionário/genética , Aneuploidia , Aberrações Cromossômicas , Cromossomos/genética , Hibridização Genômica Comparativa/métodos , Feminino , Fertilização In Vitro/métodos , Humanos , Incidência , Gravidez , Diagnóstico Pré-Implantação/métodos
AssuntosDeformidades Congênitas da Mão/genética , Hérnia Umbilical/genética , Pescoço/anormalidades , Radiografia Torácica , Costelas/anormalidades , Costelas/diagnóstico por imagem , Anormalidades Múltiplas/genética , Aberrações Cromossômicas , Cromossomos Humanos Par 14 , Evolução Fatal , Humanos , Recém-Nascido , Masculino , Fenótipo
Objective: To study the value of unmethylated cytosine guanine dinucleotide oligodeoxynucleotide (DSP30) and IL-2 in the conventional cytogenetic (CA) detection of the chromosomal aberrations in chronic lymphocytic leukemia (CLL) . Methods: Bone marrow or peripheral blood cells of CLL patients were cultured with DSP30 plus IL-2 for 72 h, following which R-banding analysis was conducted. Fluorescence in situ hybridization (FISH) was performed in 85 patients. CA results were compared with data obtained by FISH. Results: Among 89 CLL patients, the success rate of chromosome analysis was 94.38% (84/89) . Clonal aberrations were detected in 51 patients (51/84, 60.71%) . Of them, 27 (27/51, 52.94%) were complex karyotype. Among 85 CLL patients tested by FISH, chromosomal abnormalities were detected in 74 (74/85, 87.06%) patients, of which 2 (2/74) patients were complex karyotypes, accounting for 2.70%. Of the 85 CLL patients examined by FISH, 50 had abnormal karyotype analysis, 30 had normal karyotype, 5 failed to have chromosome analysis. Among them, 25 cases showed clonal aberrations by FISH assay but normal by CA, and 4 cases were normal by FISH but displayed aberrations in chromosome analysis, and totally 78 (91.76%) cases with abnormality detected by the combination of the two methods. The frequency of 13q- abnormality detected by FISH was significantly higher than that by CA analysis (69.41%vs 16.67%, P<0.001) , while the frequency of 11q-,+12 and 17p- detected by two methods showed no significant difference (P>0.05) . The detection rate of complex abnormalities in conventional karyotype analysis was higher than that in FISH (50.98%vs 2.70%) . In addition, 11 low-risk and 9 intermediate-risk patients according to FISH results showed complex karyotype by cytogenetics, and were classified into high-risk cytogenetic subgroup. Conclusion: DSP30 and IL-2 are effective in improving the detection rate of CA in CLL patients (60.71%) and CA is more effective to detect complex karyotype. However, FISH had a higher overall abnormality detection rate (87.06%) than CA, especially for 13q-. The combination of CA and FISH not only enhanced the detection rate of clonal aberrations to 91.76%, but also provided more precise prognosis stratification for CLL patients, thus to provide more information for clinical implication.
AssuntosLeucemia Linfocítica Crônica de Células B , Aberrações Cromossômicas , Citogenética , Humanos , Hibridização in Situ Fluorescente , Interleucina-2
The relative linear order of most genes on bacterial chromosomes is not conserved over evolutionary timescales. One explanation is that selection is weak, allowing recombination to randomize gene order by genetic drift. However, most chromosomal rearrangements are deleterious to fitness. In contrast, we propose the hypothesis that rearrangements in gene order are more likely the result of selection during niche adaptation (SNAP). Partial chromosomal duplications occur very frequently by recombination between direct repeat sequences. Duplicated regions may contain tens to hundreds of genes and segregate quickly unless maintained by selection. Bacteria exposed to non-lethal selections (for example, a requirement to grow on a poor nutrient) can adapt by maintaining a duplication that includes a gene that improves relative fitness. Further improvements in fitness result from the loss or inactivation of non-selected genes within each copy of the duplication. When genes that are essential in single copy are lost from different copies of the duplication, segregation is prevented even if the original selection is lifted. Functional gene loss continues until a new genetic equilibrium is reached. The outcome is a rearranged gene order. Mathematical modelling shows that this process of positive selection to adapt to a new niche can rapidly drive rearrangements in gene order to fixation. Signature features (duplication formation and divergence) of the SNAP model were identified in natural isolates from multiple species showing that the initial two steps in the SNAP process can occur with a remarkably high frequency. Further bioinformatic and experimental analyses are required to test if and to which extend the SNAP process acts on bacterial genomes.
AssuntosAclimatação/genética , Cromossomos Bacterianos/genética , Duplicação Gênica/genética , Rearranjo Gênico/genética , Seleção Genética/genética , Aberrações Cromossômicas , Evolução Molecular , Frequência do Gene/genética , Ordem dos Genes/genética , Genoma Bacteriano/genética , Modelos Teóricos , Filogenia
Propanil can produce methemoglobinemia, hemolytic anemia, hepatotoxicity, metabolic disorder and nephrotoxicity. It also has a genotoxic effect, although it is not listed as a carcinogen and it continues to be applied excessively throughout the world. Consequently, in this study the cytogenotoxic effect of propanil was evaluated, using apical root cells of Allium cepa and Lens culinaris. In which, L. culinaris seeds and A. cepa bulbs were subjected to 6 treatments with propanil (2, 4, 6, 8, 10 and 12 mg L-1) and to distilled water as control treatment. Subsequently, the root growth was measured every 24 h for 3 days. Next, the mitotic index and cellular anomalies were determined. Whereby, decreased root development was observed in all treatments. Likewise, greater inhibition of mitosis was evidenced in L. culinaris compared to A. cepa. In addition, chromosomal abnormalities, such as nucleus absence, sticky chromosomes in metaphase and binucleated cells, were present in most of the treatments. Thus, the presence of micronuclei and the results of L. culinaris, indicate the high cytogenotoxicity of propanil and the feasibility of this species as bioindicator.
AssuntosHerbicidas/toxicidade , Lens (Planta)/efeitos dos fármacos , Cebolas/efeitos dos fármacos , Propanil/toxicidade , Allium , Núcleo Celular , Aberrações Cromossômicas , Dano ao DNA , Biomarcadores Ambientais , Mitose , Índice Mitótico , Raízes de Plantas/efeitos dos fármacos , Testes de Toxicidade
OBJECTIVES: In this study, we benchmark outcomes and identify factors associated with tracheostomy placement in infants of very low birth weight (VLBW). METHODS: Data were prospectively collected on infants of VLBW (401-1500 g or gestational age of 22-29 weeks) born between 2006 and 2016 and admitted to 796 North American centers. Length of stay (LOS), mortality, associated surgical procedures, and comorbidities were assessed, and infants who received tracheostomy were compared with those who did not. Multivariable logistic regressions were performed to identify risk factors for tracheostomy placement and for mortality in those receiving tracheostomy. RESULTS: Of 458 624 infants of VLBW studied, 3442 (0.75%) received tracheostomy. Infants with tracheostomy had a median (interquartile range) LOS of 226 (168-304) days and a mortality rate of 18.8%, compared with 58 (39-86) days and 8.3% for infants without tracheostomy. Independent risk factors associated with tracheostomy placement included male sex, birth weight <1001 g, African American non-Hispanic maternal race, chronic lung disease (CLD), intraventricular hemorrhage, patent ductus arteriosus ligation, and congenital neurologic, cardiac, and chromosomal anomalies. Among infants who received tracheostomy, male sex, birth weight <751 g, CLD, and congenital anomalies were independent predictors of mortality. CONCLUSIONS: Infants of VLBW receiving tracheostomy had twice the risk of mortality and nearly 4 times the initial LOS of those without tracheostomy. CLD and congenital anomalies were the strongest predictors of tracheostomy placement and mortality. These benchmark data on tracheostomy in infants of VLBW should guide discussions with patient families and inform future studies and interventions.
AssuntosRecém-Nascido Prematuro , Recém-Nascido de muito Baixo Peso , Traqueostomia , Afro-Americanos , Aberrações Cromossômicas , Anormalidades Congênitas/epidemiologia , Permeabilidade do Canal Arterial/cirurgia , Feminino , Mortalidade Hospitalar , Humanos , Recém-Nascido , Hemorragias Intracranianas/epidemiologia , Tempo de Internação/estatística & dados numéricos , Pneumopatias/epidemiologia , Masculino , Estudos Prospectivos , Fatores de Risco , Fatores Sexuais
We report on a novel variant of the dicentric chromosome 17;20 (dic (17;20)(p11.2;q11.2) in a patient with de novo myelodysplastic syndrome (MDS). Based on FISH and array-CGH, the variant turns out to be an insertion of chromosome 17 (17p11.2-telomere 17) into chromosome 20 with breakpoints at 20q11.22 and 20q13.33. Based on conventional chromosome analysis and G-banding patterns, the region 17p11.2-17q25 was directly inserted between 20q11.22 and 20q13.33. The breakpoint junctions occurred within KCNJ12 (17p11.2), UQCC1 (20q11.2), and CDH4 (20q13.3), leading to 5' deletions of all the genes and positioning the 3' of UQCC1 next to KCNJ12 at 17p11.2 and CDH4 next to an unknown gene at 17q25-20q13.3. In addition, the centromere of chromosome 17 was not active, transforming the primary constriction to a flat band. Therefore, the novel insertion variant is a pseudo dicentric derivative chromosome with one functional centromere: 45,XX,der(17;20)del(20)(q11.22q13.33)ins(20;17)(q11.2;p11.2q25). A review of the literature of all dic(17;20) cases is presented. For the first time, we report an array-CGH characterization of such rare variant that revealed to be an insertion.
AssuntosAberrações Cromossômicas , Cromossomos Humanos Par 17/genética , Cromossomos Humanos Par 20/genética , Hibridização Genômica Comparativa , Síndromes Mielodisplásicas/genética , Linhagem da Célula , Centrômero/ultraestrutura , Bandeamento Cromossômico , Deleção Cromossômica , Feminino , Rearranjo Gênico , Humanos , Cariotipagem , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Canais de Potássio Corretores do Fluxo de Internalização/genética , Translocação Genética
To investigate the association between pathogenic copy number variants (p-CNVs) and abnormal karyotypes detected by chromosomal microarray analysis (CMA) and echogenic intracardiac focus (EIF).This was a retrospective study of fetuses with EIF with CMA data at the Prenatal Diagnosis Center of the West China Second University Hospital of Sichuan University between September 2014 and May 2017. Fetuses were assigned to the isolated EIF and non-isolated EIF groups according to the presence of other ultrasound abnormalities.Among 244 pregnant women, there were 143 cases of isolated EIF and 101 of non-isolated EIF. CMA revealed chromosome abnormality (nâ=â9 (3.7%): trisomy 21, nâ=â4; sexual trisomy, nâ=â2; and p-CNV, nâ=â3), variants of unknown significance (VOUS, nâ=â19), and benign CNV (b-CNV, nâ=â216). Among the fetuses with isolated EIF, 5 had chromosomal abnormalities (3.5%). Among the fetuses with non-isolated EIF, four had chromosomal abnormalities (4.0%). All fetuses with trisomy 21 were in the non-isolated group. The frequency of labor induction was 66.7% (6/9) among the fetuses with chromosome abnormality and 21.1% (4/19) among those with VOUS. Among those with chromosomal abnormalities, one (11.1%) had congenital heart disease.In pregnant women without high-risk factors for chromosomal abnormalities, ultrasound abnormalities, including EIF, could be an indication for CMA. Ultrasound abnormalities (including EIF) and chromosome abnormality could indicate a high risk of CHD. The presence of EIF and at least another ultrasound abnormality could indicate a high risk of trisomy 21.


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Fans all seem to know the details on how Bruce Wayne became Batman, The Dark Knight that looms over Gotham City's underworld and can probably recite them to you if asked at any point in the day, but what of the city of darkness that pulsed around him? This new show airing on Fox is entitled simply "Gotham" and it aims to give comics fans some insight on the beginnings of the caped crusader's eventually protected city and the devious villains who lurk in its shadows. Check out the poster and premise below.
Category Archives: Pop Culture
Airing Tonight: "Sleepy Hollow" Season 2 (9/22/2014)
Did you readers happen to catch the Fox series "Sleepy Hollow" last September when it made its debut? Gosh I hope so because it was a lot of quirky and supernatural fun. In keeping with our posts related to our readers interests, I am happy to announce that the second season for the show will begin tonight. Check out one of the promotional posters below and then we can refresh the minds that saw it or inform those who missed out.
Continue reading Airing Tonight: "Sleepy Hollow" Season 2 (9/22/2014)
DC Comics Presents: The New 52 "Futures End" Week Three
Keeping up with the program of the epic multi-title "Futures End" storylines, here I am with the third week of offerings from DC Comics. Have you been following along so far? Some good stuff in the past two weeks for sure and here now is the third grouping.
FUTURES END IS HERE! Welcome to the future – FUTURES END, that is! This September, DC Entertainment takes you five years into the future of DC Comics – The New 52, where the heroes of the DC Universe face challenges unlike anything you've ever seen!
TEEN TITANS: FUTURES END #1: With the Teen Titans dead, a new group of young heroes seek justice in the future DC Universe!
JUSTICE LEAGUE UNITED: FUTURES END #1: In the concluding chapter of the epic story that began in this month's JUSTICE LEAGUE: FUTURES END #1, the armies of Mars rise against the unsuspecting population of Earth – and only the combined might of the Justice League and the Legion of Super-Heroes can stand against them!
Continue reading DC Comics Presents: The New 52 "Futures End" Week Three
Out Now: "Aliens: Fire and Stone" #1
About The Comic: During a vicious xenomorph outbreak, terraforming engineer Derrick Russell leads a desperate group of survivors onto a rickety mining vessel. They hope to escape the creatures overrunning their colony—but they'll face horrors both in space and on the strange planet they crash on. Ties in with the Prometheus and Aliens films!
PiercingMetal Thoughts: I've always enjoyed the Aliens comics from Dark Horse because they give you what you crave the most about the creatures. Their utter ruthlessness and savagery upon mankind and well, any sort of kind that is in front of them. To the Xenomorphs we are all food and I'm glad they don't really exist. Now while I will likely peruse the title when I see it on the racks to enjoy the artwork, I am not planning on paying much attention to it until a collected edition is out. I'm trying to slow down on the single issue purchases these days outside of the digital copies I see for reviewing and sharing with you folks purposes. I would much rather see this in my bookcase next to similar tomes. What do you readers think of this massive crossover? Are you down with it or not? Let me know in the comments section below.
Official Website: http://www.darkhorse.com
Out Now: "Prometheus: Fire and Stone" #1
About The Comic: When the Prometheus never returned from her fateful journey to LV-223, the questions surrounding the origins of man went unanswered. Now a new team of explorers seeks to uncover the dark mystery that holds not only the fate of theoriginal mission, but possibly their own damnation. Ties directly into the hit motion picture! First issue in a blockbuster crossover event!
PiercingMetal Thoughts: While I love that Dark Horse Comics is going to be doing a connecting series of titles to expand upon this story line, I must share with you that I absolutely HATED this "Prometheus" film. Now, a lot of my friends thought it was amazing but I felt it was too long, too ponderous and really only offered up a small amount of "jeepers" moments. To each their own right? All I know is that I am NOT adding this film to my video library nor will I ever watch it again. With that being said I will peruse the comic when its on the shelves of my local shop but I don't think that I am going to buy the single issues and instead shall wait for the collected edition. This is going to cross into a title with Aliens and Predator franchises so in a few months I will have some pretty creepy reading to do. What do you readers think about the idea of this "Prometheus" comic series and it connecting to the other character universes? Let me know in the comments section below.
Official Website: http://www.darkhorse.com


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text: Before You Sell Your Car: How to Get Your Best Sale Price #infographic… by Simpson Michael J. Before You Sell Your Car: How to Get Your Best Sale Price #infographic Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window) Related See more Previous article Introducing Online Loans: From Conexus & Grow Next article Business Line Of Credit Loan Leave a Reply Cancel replyYour email address will not be published. Required fields are marked *Comment Name * Email * Website Save my name, email, and website in this browser for the next time I comment. Notify me of follow-up comments by email. Notify me of new posts by email.


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Paul J. Mills, Tiffany Barsotti, Meredith A. Pung, Kathleen L. Wilson, Laura Redwine, and Deepak Chopra Gratitude, along with love, compassion, empathy, joy, forgiveness, and self-knowledge, is a vital attribute of our wellbeing. While there are many definitions of gratitude, at its foundation, gratitude is a healing, life-affirming, and uplifting human experience that shifts us […]
Millions of people today are following the path of self-help–buying books, joining groups, going away on retreats–all in the hopes of transforming their lives. But is this mass of information really getting us anywhere? Deepak Chopra sorts through the hype to uncover the real benefits of self-help and how you can best use it to change your life. Read more at Oprah.com
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Deepika Padukone was the favourite to win the Best Actress award at various award ceremonies for her spectacular performance in Padmaavat but she hasn't been always lucky.
In fact, recently she lost out the trophy at Filmfare Award night to Alia Bhatt who won it for Raazi. while Deepika Padukone does not take it to heart, the news didn't go down well with many of her fans who slammed Filmfare for their alleged prejudice against Deepika.
They even questioned how Ranveer Singh and Ayushmann Khurrana shared Best Actor Critics award but when it came to Best Actress category, the same rule wasn't followed.
Deepika got the wind of it and responded to the fan club to end of the backlash. She commented, "To all my Fans, I am sorry I let you all down and promise to work harder than ever before… #upwards&onwards."
ALSO READ: 'Chhapaak' First Look Out: Deepika Padukone's Transformation Into An Acid Attack Survivor Is Unrecognisable
The actress posted the comment just as she dropped the first look of Chhapaak. The actress has been receiving great appreciations from her peers in the industry. Stars like Vicky Kaushal and Alia Bhatt applauded Deepika's look in the movie.
In Chhapaak she plays the role of an acid attack survivor named Malti. The film is based on the life of acid attack survivor Laxmi Agarwal and will be extensively shot in Delhi. Starring Vikrant Massey as the male lead, Chhapaak is slated to release on January 10, 2020.
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