text:
Aug 15 1979
Poco Setlist at Garden State Arts Center, Holmdel, NJ, USA
Sorry, there are no songs in this setlist yet, but ...
If you were there then
add whatever song you remember!
And you might also get help in the
setlist request forum
Edits and Comments
1 activity (last edit by ExecutiveChimp, 25 May 2020, 01:09 Etc/UTC)Show edits and comments
Garden State Arts Center, Holmdel, NJ, United States
Poco Gig Timeline
Previous concerts
- Poco Music Inn, Stockbridge, MA - Aug 12, 1979 Aug 12 1979
- Poco Calderone Concert Hall, Hempstead, NY - Aug 14, 1979 Aug 14 1979
Aug 15, 1979
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A DRIVER stopped for having no insurance told police she had ignored insurance companies' letters and emails.
The driver of a Land Rover was stopped in Dorchester Road, Poole, and the vehicle was seized.
A spokesperson for the Dorset Police No Excuse team said: "A Land Rover was seized for no insurance the driver stated she'd ignored the insurance companies' letters and emails.
"Not wise, it's cheaper in the long run to take out insurance."

subset: commoncrawl


text:
T.L. Whiteside
Editorial Board, Journal of Immunological Methods Pittsburgh, Pennsylvania, United States
Theresa L. Whiteside, Ph.D. is Professor of Pathology, Immunology and Otolaryngology at the University of Pittsburgh. She is a tumor immunologist with over 30 years of experience in immunology research and immune monitoring, and her expertise is recognized worldwide. For 25 years, she served as Director of the Immunologic Monitoring and Cellular Products Laboratory at UPCI. She has participated in numerous clinical trials and has published extensively. Dr. Whiteside was one of the first investigators to call attention to compromised functions of T lymphocytes and natural killer cells in the microenvironment of human tumors. She has mentored over 80 postdoctoral fellows.


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Nashville-based FirstBank, a wholly owned subsidiary of FB Financial Corporation (NYSE: FBK), is the third largest Tennessee-headquartered bank, with 45 full-service bank branches across Tennessee, North Alabama and North Georgia, and mortgage locations across the Southeast. The bank serves five of the major metropolitan markets in Tennessee and, with approximately $5 billion in total assets, has the resources to provide a comprehensive variety of financial services and products.
FirstBank was the proud sponsor of the Thanksgiving Day Boulevard Bolt in Nashville, TN. The Boulevard Bolt is a 5K race that raises money and awareness for organizations fighting homelessness in Middle TN.
Several FirstBank associates participate in volunteer programs such as Big Brothers, Big Sisters. This is David Bocangel from our Manufactured Housing division with his little brother in the program.
Associates from our Bruceton-Hollow Rock branch serving lunch at the local high school.
FirstBank Manchester Branch


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- a year ago
@NipseyHussle @Kodaklens1 @NipseyHussle needs a Holiday name after him like #MLK Name A Street After Him Make him a DAY and Give Section in Crenshaw Day in california to unite people like he did with rival gangs @LAPDChiefMoore Make Petition for Nipsey to have holiday in his honor @LaurenLondon102


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Kenny Perry and Fred Funk made charges in the U.S. Senior Open on Saturday, each pulling within two shots of leader Michael Allen with one round to go.
Perry, going for a second straight win in a senior major, shot 6-under-par 64 to match Corey Pavin for the low round at Omaha Country Club. Funk shot 67 after making a long birdie putt on No. 18.
Allen, who had a 63 Friday, ballooned to a 2-over 72. He started the day with a 5-stroke advantage over Rocco Mediate. It was the largest lead after 36 holes in the tournament's 34-year history.
Allen was at 8-under 202 for the tournament.
Pavin, who tied for second in last year's tournament, was two shots behind Perry and Funk and four behind Allen.
Mediate faded with bogeys on four of five holes in the middle of his round and sat five shots off the lead after a 72.
Stenson holds off Mickelson in Scotland
INVERNESS, Scotland -- Henrik Stenson held off a resurgent Phil Mickelson at the Scottish Open by shooting a 6-under 66 to take a two-stroke lead into the final round.
Mickelson was in a group of four players in a tie for second after making six birdies in his last 11 holes for a 66 on Saturday. His performance has the American talking up his improving links game before next week's British Open.
Branden Grace and first-round leader John Parry were tied with Mickelson after each shooting 66, along with J.B. Hansen (69) .
Stenson, at 16 under for the tournament, has emerged from a prolonged slump and is showing the kind of consistent form that took the Swede to No. 4 in the world four years ago.
Summerhays grabs 2-shot lead
SILVIS, Ill. -- Calm skies and a near-perfect course gave every golfer at the John Deere Classic the chance to shoot a really low number on Saturday.
Daniel Summerhays went lower than everyone else, seizing firm control heading into today's final round.
Summerhays shot a 9-under 62 for a two-stroke lead following third-round play. Summerhays, whose previous best finish on the PGA Tour was a tie for fourth, enters the final round at 19-under 194 and in position for his first career win.
He notched 10 birdies while matching the lowest third-round score in tournament history.
Canadian David Hearn (64) is second at 17 under. Defending champion Zach Johnson held a share of the lead after each of the first two rounds, but he's now alone in third after shooting a 67.
J.J. Henry and Jerry Kelly are tied for fourth at 15 under, while Nicholas Thompson leads three golfers at 14 under.
Summerhays had missed three straight cuts -- failing to shoot a round under 70 in those events -- before finishing ninth last week at the Greenbrier Classic.
Park shoots 61 to take lead at Manulife
WATERLOO, Ontario -- South Korea's Hee Young Park shot a career-best 61 on Saturday to move into the lead after the third round at the Manulife Financial LPGA Classic.
Park was 10 under for the round and 20-under 193 overall, one stroke better than American Angela Stanford and two shots ahead of Scotland's Catriona Matthew.
Allen's lead down to 2 at U.S. Open
Kenny Perry and Fred Funk made charges in the U.S. Senior Open on Saturday, each pulling within two shots of leader Michael Allen with one round to go.


text:
Dark Chocolate by Foray
THC distillate (indica dominant)
Package contains: 2.5mg of THC per square x 4 squares (10mg total)
Ingredients: 66% Dark Chocolate (unsweetened chocolate; sugar; cocoa butter; soy lecithin; vanilla beans seeds), Cannabis Extract.
I've heard many good things about Foray edibles and this didn't disappoint. This is a wonderfully decadent but not too sweet chocolate that's simply delightful. Foray uses a high quality chocolate and you can tell. It's not just a nice chocolate on its own, it provides a calming elevation that's perfect for when you need to unwind and relax. This is a good one for anyone who has issues with anxiety.
*note- I found these chocolates to be a little strong, if you're new to cannabis or edibles, start with one square.
It's self care in a chocolate
I will absolutely be buying this chocolate again, it's one of the best edibles I've had and I would recommend Foray Dark Chocolate to anyone who appreciates good quality chocolate. The only thing that might be a downside for some of you is the size of each piece of chocolate. This might seem a bit too much to eat if you need to eat all 10mg, however you could also grate this chocolate into some warm milk for a drinkable chocolate. If you're new to cannabis or only need a low dose and love chocolate, this is the perfect thing.


text:
Stay informed
Subscribe to WA ScamNet alerts
Subscribe to FuelWatch alerts
Make a consumer complaint
Contact our translation service
Like us on Facebook
Follow us on Twitter
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The Commissioner for Consumer Protection has publicly released a list of 20 stores in WA which have been identified as selling dangerous unapproved USB plug-in chargers and adapters and warns their customers to carry out an urgent safety check.
Product safety and regional officers from Consumer Protection, under the supervision of EnergySafety, have completed a two-week extensive search for unapproved chargers being sold in WA. The search covered the Perth metropolitan area and regions around Albany, Broome, Bunbury, Geraldton and Karratha.
The blitz was launched after the tragic death of a NSW woman. She was electrocuted while using headphones plugged into her laptop and talking on her mobile phone when it was being charged using an unapproved 240V ac charger. There was a local case last month where a woman received an electric shock at her Naval Base workplace while using one of these unapproved chargers, a number of which closely resemble well-known brands.
Commissioner for Consumer Protection Anne Driscoll said the joint operation involved officers checking 144 retail outlets in WA, removing unsafe devices from sale.
"A total of 20 traders have been found to be selling 25 unapproved and potentially deadly USB chargers and adapters, ranging in price from $5 to $25. At least 30 other questionable devices also have been given to EnergySafety for further investigation," Ms Driscoll said.
"The reason for releasing the list of stores is to alert their customers who may have bought chargers or adapters recently so they can be aware and check the devices immediately to make sure they are approved and comply with Australian standards.
"The stores have cooperated by removing the devices identified from sale, but our major concern is that these potentially deadly USB chargers and adapters could be in use in WA homes and workplaces, posing a serious risk to consumers.
"While we are confident that we have inspected all likely retail outlets, there may be some stores that could still be selling these dangerous products that we are not aware of, and they could also be available for sale on the internet. All consumers who have bought these products in recent months are urged to check that their device has Australian approval markings, has insulated plug pins and is safe to use."
The stores and products identified include:
|Red Finger
|Thornlie Square Shopping Centre
|USB Power Supply Charger
|Creative House
|Coventry Village Morley
|International adapter All-in-one
|Coventry Gifts and Gadgets
|Coventry Village Morley
|2 Port USB 2.1A charger
|Creative House
|Malaga Markets
|2-port USB charger LED display
|The Aussie Man Cave Toys and Gifts
|Gosnells Railway Markets
|Universal travel adapter
|Coventry Village Morley
|AC adapter KS-878
|Tan Ly
|Canning Vale Markets
|Unmarked charger
|Wotel Wangara
|Wanneroo Markets
|T3-1000 power adapter
|Mobile Solution
|Mirrabooka Shopping Centre
|USB power adapter
|Mobile Tech
|Midland Gate Shopping Centre
|Samsung Black Type
|Mobile Tech
|Warwick Grove Shopping Centre
|USB 240V Adapter1910294 4590
|Accessories World
|Kingsway Shopping Centre
|Universal Adapter
|Fix N Shop
|Kiosk 6 The Shops Ellenbrook
|USB charger adapter A1265
|I Workshop
|Perth Underground (top level)
|Samsung charger
|Doogues Lucky Lotteries
|Murray Street Mall, Perth
|Jama Lifestyle Universal Home and Car Charger
|Haiou Phone Repairs
|Midland Gate Shopping Centre
|USB charger A1265
|Phix Carousel
|Carousel Shopping Centre
|Mobile Tech
|Ocean Keys Shopping Centre, Clarkson
|4-ports USB charger
|Mister Money
|North Road Shopping Centre, Albany
|Entertainment Konnections
|Paspaley Shopping Centre, Broome
|Product identified
Director of EnergySafety Ken Bowron said consumers who have bought USB chargers and adapters from these outlets in recent months should check for Australian approval markings and, if unapproved, they should stop using, destroy and dispose of them immediately.
"Consumers should always buy a well-known reputable brand of charger or adapter that meets Australian standards. They should check that the plug pins are of an Australian configuration, are insulated at the base of the pins and have Australian approval markings," Mr Bowron said.
"We also warn against using mobile devices while they are charging and advise not to leave them plugged in on charge overnight.
"Suppliers and retailers have an obligation to ensure that the electrical products they sell are compliant with Australian safety standards and there are serious consequences for businesses found selling unapproved devices, with a maximum penalty of $50,000 for an individual or $250,000 for a corporation."
Unapproved chargers and adaptors should be destroyed and disposed of immediately. If they are found to be on sale, contact either Consumer Protection or EnergySafety.
Examples of approval markings and numbers can be found on the EnergySafety website: www.commerce.wa.gov.au/energysafety/electrical-appliance-approval-marks
Consumer tips:
Photos of some of these products appear below.
Example 1
Example 2
Example 3
Media Contact (EnergySafety or Consumer Protection).
Last modified:


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There's a good chance there are a few customers that aren't paying you enough and generate most of your stress.
Figure out who they are, fire them and spend that energy growing your business and finding more of your ideal clients.
I started the Sweaty Startup in December of 2018 because I believe the Shark Tank and Tech Crunch culture is ruining the real spirit of low-risk entrepreneurship.
No spam. No BS. Just short actionable emails once a week to help you build a better business.


text:
Cindy Hawthorne
Cindy Hawthorne is a noted blogger who has thorough knowledge about the Business and startup. You can follow her blogs on the same to know more about the conceptions. She has also worked as a Home Décor Expert.
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Adorable floral arrangement ideas for your home decoration
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National United Church Ushers Association of America, Inc. (NUCUAA) - Celebrating Over 100 Years!
The 2019 Centennial Convention is over, but you can still experience some of the excitement we felt. Some selected videos and pictures taken at the convention are posted below (after the 101st National Convention information).
For more information regarding our parent site, whether it is what the Association has to offer you or convention information, feel free to visit their website at https://www.nationalchurchushers.org/
NUCUAA's 101st Convention
July 27-31, 2020
Rosen Centre Hotel
9840 International Drive
Orlando, FL 32819
Room Rate: $139.00 plus tax (currently 13.63%, subject to change); total cost per room, per night is $157.95.
Deadline for reservation: Monday, July 6, 2020. Everyone is encouraged to reserve your room as soon as possible. Group Name: NUCUAA
Hotel reservations can be made via our National website at https://www.nationalchurchushers.org. On the homepage, click on NUCUAA.
Reservation Guarantee: In order to guarantee a reservation, the hotel requires that attendees provide a deposit equal to one night's room and tax to be submitted at the time of reservation. This charge of one night's room and tax will be applied as a credit to the guest bill upon checkout. Should there be an invalid or declined credit card, the reservation will be canceled.
Refundable Deposit: Deposits are refunded in full if a reservation is canceled by June 1, 2020.
The Housing Chairman information will be updated soon. For questions or problems contact National Board of Directors Chairman Melinda Wilkins at email@example.com.
Online Pre-Registration: Pre-Registration is now open, you can go to or click here.
Deadline for pre-registration: is June 15, 2020. All State financial obligations will also be handled on this link as well.
Transportation Options:
Below is the information for the Delta Airlines discount.
The meeting event code for the NUCUAA National Convention 2020 is NMU6N. Reservations and ticketing are available via www.delta.com. Select 'Book Your Flight' and this will bring you to the 'Book A Flight' page. Enter the meeting code in the box provided.
Reservations may also be made by calling the Delta Meeting reservations at 800-328-1111 Mon-Fri 7 am-7 pm CDT. Again, reference the meeting event code NMU6N. *Please note that there is a direct ticketing fee for booking through the stated reservation number.
Discounts on Delta or Delta Codeshare Flights can range from 2%-10% depending on where the passenger is traveling from. Once ticketed, if changes occur, an administrative fee may apply in addition to any difference in the fare.
Southwest Airlines information is below.
Attendees of NUCUAA can take advantage of discounted fares when traveling to/from Orlando. Book your flights in SWABIZ using Company ID (99626310) to qualify for special rates and increased Rapid Rewards earning. Discounts are eligible for reservations booked February 1st through July 5th for travel July 19th through August 3rd.
If you have any questions, please contact Board of Directors Chairman: Melinda Wilkins via her email address firstname.lastname@example.com.
Ground Transportation: Contact Mears Share A Ride at the Orlando International Airport. Call (1-800-759-5219) for more information.
Souvenir Journal:
Souvenir Journal Ad submission deadline is May 1, 2020. Please follow the guidelines submitted by our Souvenir Journal Chairman, Director Travis Harden. Below is the information regarding the ads. Ads go to and monies will go to George Jones, Assistant Financial Secretary; email Brother Jones at to get his mailing address. Monies must be received before your ad is placed in the Souvenir Journal. You will receive a FREE SOUVENIR JOURNAL WITH EACH FULL-PAGE AD! Ad amounts are as follows:
____ NATIONAL OFFICERS $25.00 (Seniors & Young Adults, Past & Present)
____ NATIONAL OFFICERS $15.00 (Junior & Young People, Past & Present)
Vendor Contracts are available on the National web site. Please let those who want to be a Vendor know to contact Director Warren Willis at email@example.com. A deposit of $100 must be made with application prior to the Convention and full payment received by June 30, 2020.
Scooters & Wheelchairs:
Scooters and wheelchairs will be available again this year. The amount of the scooter rental is $195.00 for the week. A 50% deposit is due upon making your reservation. Balance must be paid by June 1, 2020, prior to your arrival to the Convention. Please contact Director, George Merida at if you need a scooter and forward your deposit to Financial Secretary Roslyn Moore, email Sister Moore at to get her mailing address. This payment can also be made online with your registration.
National Officer (Nominations):
Contact your State President to obtain a copy of the required Resume Format and Consent Form to be submitted by those intending to run for a National Office at this year's convention. State Presidents also have a copy of the Duties for Elected Officers, which includes a list of those who are currently serving in the positions and a note as to whether or not they are eligible for re-election.
The National deadline to submit these forms to your Regional Nominating Chairman is April 30, 2020. State Presidents should also have information on the Region's deadline to receive nominations for action at the proper Regional Conference. Region representatives are listed below.
Shirley Sisson Davis - Central Region
Jeannette S. Riddick - Eastern Region
Rosemary Stevens - Southern Region
Billye R. Woodle - Western Region
National Outreach Project:
The Outreach Project for 2020 are backpacks and gift cards for two middle-schools in Orlando, Florida. Selection of the schools have not been provided at this time.
Young Adult Department Fundraiser:
The special projects for the Young Adult Departments is a power bank. Cost is $20.00. If there are more requests for items than they have available, they will accept paid pre-order sales for them.
Junior & Young People's Department Fundraiser:
The Junior & Young People's special project is a Junior Department jacket. Additional information will be available as soon as possible. Sales will be based on pre-orders sales only.
Other Junior & Young People's Event Information:
The Junior Department outing to Universal Studios is scheduled for Wednesday of the convention. The cost with transportation is $100. Tickets must be purchased through online pre-registration on the NUCUAA website. There will be NO ADDITIONAL TICKETS AVAILABLE ON-SITE. Permission slips are required prior to attending and participating in the Wednesday outing. If National Supervisors do not have a signed permission slip, the young people will not be able to participate. No exceptions. Permission slips and all required forms are expected to be sent out by April 1, 2020.
The Evening of Elegance event is $20.00. Tickets will be available only through online pre-registration. This also applies to all who may may attend either or both activities. No money will be accepted for these activities at the site of the convention. Please adhere to these requirements so that the Board of Directors can adequately prepare.
Background Checks:
Supervisors attending the Junior and Young People's meetings or any activities with the Junior Department MUST have a background check prior to entering their meetings at the National Convention. If you have an approved background check from last year (2019) you will not have to complete another background check this year. Background checks are good for two (2) years. Request for background checks should be sent to Chairman, Melinda Wilkins at email@example.com.


text:
What Will Summer Be Like Without the Usual VBS, Camps and Youth Mission Trips?
Christian professionals and families partner to experiment with innovative approaches to youth formation in a socially distant environment.
Christian professionals and families partner to experiment with innovative approaches to youth formation in a socially distant environment.
Earlier this month, Phil Vischer — creator of "VeggieTales" and voice of Bob the Tomato in the popular Christian animation series — posted a video that attempts to answer the question: "Why are people so angry?"
A census is not a spiritual matter, but numbers play an important role in church life. Numbers are a bit like a temperature. It is a first line of judgment but not the only one.
A Tennessee newspaper said Sunday it is investigating what its editor called a "horrific" full-page advertisement from a religious group that predicts a terrorist attack in Nashville next month.
Barbara Nell "Babs" Baugh as she was laid to rest in Houston, Texas on Saturday (June 20) in a service officiated by Garrett Vickrey, senior pastor at Woodland Baptist Church in San Antonio, Texas; Daniel Vestal, former executive coordinator of the Cooperative Baptist Fellowship; and
Word&Way Editor Brian Kaylor preached Sunday (June 21) at First Baptist Church in Columbia, Missouri. He considered the story of David, a census, and a plague as found in 1 Chronicles 21.
With COVID-19 restrictions preventing an intended in-person rally in Washington D.C., at least a million supporters of the Poor People's Campaign reportedly tuned in Saturday (June 20) to watch a mix of live speeches and pre-recorded clips of liberal religious leaders calling for a "moral
Southern Baptist pastor Alan Cross reflects on controversial comments on slavery by Louie Giglio and says that perhaps it's time for white American evangelicals to rethink what privilege and blessing mean to us.
On June 19, 1866, Texas freed-persons celebrated the anniversary of the announcement of their freedom — the first Juneteenth holiday. This year, the holiday helps highlight a Baptist pastor & George Floyd.
First Baptist Church of Crestmont in Willow Grove, Pennsylvania, is among several Southern Baptist congregations celebrating for the first time Juneteenth, or June 19th, the day in 1865 when enslaved black people in Galveston, Texas, learned they were free.


text:
We have full episodes of Almost Royal season 2 tv series in high quality (hd). Our links have no ads and are completely SAFE DOWNLOADS, no torrents! Scroll this page to get direct links. Downloading of Almost Royal (season 1, 2, 3) for mobile, pc, android & Iphone(iOS).Stream NOW
Comedy TV Series Almost Royal (season 1, 2, 3) was released in 2016-01-18 by BBC America Channel. Serial was created by , directed by . Cast include Ed Gamble, Amy Hoggart, Gemma Whelan. The avarenge rating on IMDB: is 7.2/ 10.
Series info
Download links
Episode 1
720p-HDTV: (.mkv, 531.4 MB) |
(.mp4, 177.3 MB) |
Episode 2
720p-HDTV: (.mkv, 738.7 MB) |
(.mp4, 249.0 MB) |
Episode 3
720p-HDTV: (.mkv, 630.6 MB) |
(.avi, 344.7 MB) |
(.mp4, 212.4 MB) |
Episode 4
720p-HDTV: (.mkv, 547.2 MB) |
(.avi, 280.7 MB) |
(.mp4, 176.6 MB) |
Episode 5
720p-HDTV: (.mkv, 620.0 MB) |
(.avi, 329.3 MB) |
(.mp4, 206.9 MB) |
Episode 6
720p-HDTV: (.mkv, 588.3 MB) |
(.avi, 304.1 MB) |
(.mp4, 191.9 MB) |
Episode 7
720p-HDTV: (.mkv, 687.7 MB) |
(.avi, 354.6 MB) |
(.mp4, 214.2 MB) |
Episode 8
720p-HDTV: (.mkv, 628.6 MB) |
(.avi, 328.0 MB) |
(.mp4, 197.0 MB) |
Other seasons of the show
Almost Royal season 2 direct download links
Episode Guide
air day: 2016-01-18
Poppy as well as Georgie Carlton have actually gone back to the United States as well as are considering the art of appeal. The season best sees them going to a college to discover how to implement the best blow-dry, evaluating an attractive child competitors as well as selflessly adding to globe tranquility by participating in an appeal contest. American TELEVISION host Mario Lopez gets on hand to address the brother or sisters' concerns on every facet of appeal.
The Outdoors
air day: 2016-01-18
The Carlton's check out the open airs, which sees them standing up close as well as extremely individual with some Floridian wild animals, treking a path with some actual Americans, conserving lives on a preferred coastline as well as lastly sharing their very own extremely specific brand name of compassion with a team of militants. Broadway celebrity as well as starlet Laura-Bell Bundy is assisting the aristocrats to improve any type of as well as all misconceptions.
air day: 2016-01-25
In this episode, so-called investigatory YouTube reporters Poppy as well as Georgie are considering the globe of American sporting activities by attempting their hand at being experts on a TELEVISION program, coming to be a mascot for an university basketball group, going to a drag-race track prior to attempting the sporting activity of Quidditch. Professional info is supplied by basketball tale Dennis Rodman.
air day: 2016-01-25
Ambitious on the internet analysts Poppy as well as Georgie have love on their minds. Recommendations on all points enchanting originates from star Michael Urie (The Great Partner, Ugly Betty).
air day: 2016-02-01
Siblings Poppy as well as Georgie get on a goal to discover exactly what the future holds for the mankind, beginning with a substitute journey to Mars. Successive they fulfill individuals that are wishing to endure the armageddon prior to signing up with a political prospect with an extra enthusiastic vision. Celebrity Expedition tale George Takei overviews them on their mission.
air day: 2016-02-01
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air day: 2016-02-08
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Combination of ferric ammonium citrate with cytokines involved in apoptosis and insulin secretion of human pancreatic beta cells related to diabetes in thalassemia
- Published
- Accepted
- Received
- Academic Editor
- Gwyn Gould
- Subject Areas
- Biochemistry, Cell Biology, Diabetes and Endocrinology
- Keywords
- Ferric ammonium citrate, Cytokines, Pancreatic beta cell, Thalassemia, Diabetes, Insulin, Apoptosis, Reactive oxygen species, ER stress, Iron overload
- Copyright
- © 2020 Rattanaporn et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
- Cite this article
- 2020. Combination of ferric ammonium citrate with cytokines involved in apoptosis and insulin secretion of human pancreatic beta cells related to diabetes in thalassemia. PeerJ 8:e9298 https://doi.org/10.7717/peerj.9298
Diabetes mellitus (DM) is a common complication found in β-thalassemia patients. The mechanism of DM in β-thalassemia patients is still unclear, but it could be from an iron overload and increase of some cytokines, such as interleukin1-β (IL-1β) and tumor necrosis factor-α (TNF-α). The objective of this study was to study the effect of interaction between ferric ammonium citrate (FAC) and cytokines, IL-1β and TNF-α, on 1.1B4 human pancreatic β-cell line.
The effect of the combination of FAC and cytokines on cell viability was studied by MTT assay. Insulin secretion was assessed by the enzyme-linked immunosorbent assay (ELISA). The reactive oxygen species (ROS) and cell apoptosis in normal and high glucose condition were determined by flow cytometer. In addition, gene expression of apoptosis, antioxidant; glutathione peroxidase 1 (GPX1) and superoxide dismutase 2 (SOD2), and insulin secretory function were studied by real-time polymerase chain reaction (Real-time PCR).
The findings revealed that FAC exposure resulted in the decrease of cell viability and insulin-release, and the induction of ROS and apoptosis in pancreatic cells. Interestingly, a combination of FAC and cytokines had an additive effect on SOD2 antioxidants' genes expression and endoplasmic reticulum (ER) stress. In addition, it reduced the insulin secretion genes expression; insulin (INS), glucose kinase (GCK), protein convertase 1 (PSCK1), and protein convertase 2 (PSCK2). Moreover, the highest ROS and the lowest insulin secretion were found in FAC combined with IL-1β and TNF-α in the high-glucose condition of human pancreatic beta cell, which could be involved in the mechanism of DM development in β-thalassemia patients.
One of the hereditary blood disorders resulting from a defect in β-globin chain synthesis is β-thalassemia, which is caused by point mutation or, more rarely, deletion of the β-globin gene (Galanello & Origa, 2010). These defects cause an imbalance between α and β-globin chains leading to ineffective erythropoiesis (IE) chronic hemolytic anemia. Extramedullary erythropoiesis is caused by β-thalassemia, which leads to bone deformities and hepatosplenomegaly. Many complications were found such as heart failure, cirrhosis, and endocrine complications including diabetes mellitus (DM) (Weatherall, 2003). The prevalence of DM has been reported by up to 40% in β-thalassemia patients (Ghergherehchi & Habibzadeh, 2015; Li et al., 2014; Liang et al., 2017; Metwalley & El-Saied, 2014).
Patients with β-thalassemia and DM have a higher risk of cardiovascular complications (Pepe et al., 2013). However, the mechanism of DM in β-thalassemia patients is still unclear. Several mechanisms involved in DM development in β-thalassemia patients were reported including insulin resistance, hepatic dysfunction, and insulin deficiency resulting from β-cell damage or apoptosis (Noetzli et al., 2012; Ghergherehchi & Habibzadeh, 2015; Li et al., 2014). However, there is no report linking iron and those mechanisms in β-thalassemia patients.
Pancreatic β-cell damage is caused by many factors such as hyperglycemia, ROS, pharmacological factors, environmental toxicity factors, tumors, chronic pancreatitis, infections, inflammation, and autoimmunity. ROS caused by iron leads to damaged cells via the Fenton reaction. Iron is thought to cause cell death through ROS-dependent and -independent mechanisms (Dixon & Stockwell, 2014; Bogdan et al., 2016). Although the iron metabolism in pancreatic β-cells is still unclear, it is believed that pancreatic islet cells are susceptible to oxidative damage because they express low levels of antioxidants (Tiedge et al., 1997). One study of iron regulation in pancreatic islet cells reported that high expression of divalent metal transporter (DMT1) caused more iron deposits in pancreatic cells than other cells (Andrews, 1999).
In β-thalassemia patients, iron might not be the only factor that causes β-cell damage. Increased levels of inflammatory cytokines have been reported in β-thalassemia patients, especially TNF-α and IL-1β with mean levels 3- and 28-times higher than normal controls, respectively (Wanachiwanawin et al., 1999). These might cause β-cell damage through apoptosis signaling pathways in β-thalassemia patients. In addition, cytokines, especially IL-1β, also have the potential to increase intracellular iron and ROS, which cause apoptosis as has been described in insulin-producing cells (Hansen et al., 2012). Therefore, the increase of both iron and cytokines might be an important mechanism of DM in β-thalassemia patients. Here, the combined effect of iron and cytokines on the human pancreatic β-cell line is examined.
Materials and Methods
Pancreatic cell culture
The human pancreatic β-cell line 1.1B4 was generated by electrofusion of freshly isolated human pancreatic beta cells and the human PANC-1 epithelial cell line. It showed glucose sensitivity and responsiveness to known modulators of insulin secretion (McCluskey et al., 2011). The 1.1B4 cells passage 28 (ECACC Cat. No. 10012801) were routinely cultured in pre-warmed RPMI640 culture medium with L-glutamine containing 11.1 mM glucose (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% v/v fetal bovine serum (Merck, Burlington, MA, USA) and 10 U/ml penicillin and 0.1 g/l streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in 5%CO2.
1.1B4 cell treatment with FAC, cytokines and high glucose
The 1.1B4 cells were plated on six well plates with or without high glucose condition (22.2 mM glucose) (Vasu et al., 2013) and allowed to attach into cell plates for 24 h. Then, they were treated with different concentrations of FAC (Merck) or IL-1β or TNF-α (Cell Signaling Technology, Danvers, MA, USA) for 12 and 24 h.
Cell viability analysis by MTT assay
MTT assay (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) (Thermo Fisher Scientific, Waltham, MA, USA) was used to compare the effect of FAC, IL-1β, and TNF-α on cell viability. The 1.1B4 cells were plated on 96 well plates and allowed to attach plates for 24 h. After the treatment, the culture medium was removed, and 100 μl of culture medium was added. Then, ten microliters of 12 mM MTT was added and incubated for 4 h at 37 °C. Finally, 85 µL MTT-containing medium and 50 µl DMSO was added to each well and analyzed at 540 nm by a SpectraMax 200 (Molecular Device, San Jose, CA, USA).
Analysis of intracellular ROS by flow cytometer
Intracellular ROS was detected by 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Merck, Kenilworth, NJ, USA); the cells were trypsinized and washed twice with Ca2+ and Mg2+-free Dulbecco's phosphate buffer saline, then transferred to polypropylene (PP) tube. Then 0.05 mg/mL of DCFH-DA was added into the tube and incubated for 15 min at 37 °C, 5% CO2. The fluorescence signal was detected by a fluorescence-activated cell sorting (FACS) Canto flow cytometer (BD Biosciences, San Jose, CA, USA) and was analyzed by FACSDiva software (BD Biosciences, San Jose, CA, USA).
Analysis of apoptotic cells by flow cytometer
The apoptosis cells were detected using Annexin V: FITC Apoptosis Detection Kit I (BD Biosciences, San Jose, CA, USA). The cells were washed and incubated with fluorescein isothiocyanate (FITC) conjugated Annexin V (AnV) and propidium iodide (PI) at room temperature for 15 min. Fluorescence was detected by a FACSCanto flow cytometer and analyzed with FACSDiva software (BD Biosciences, San Jose, CA, USA).
Gene expression by RT-qPCR
Total RNA was extracted from 1.1B4 cells using TRIzol reagent (Invitrogen, Calsbad, MA, USA). Then, the conversion of 5 µg of total RNA to cDNA using RevertAid kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions, the qPCR was performed using SYBR green. The Select Master Mix for CFX (Applied Biosystem, Foster City, CA, USA) was used for RT-qPCR, through specific primers, and run on CFX96 Touch Real-Time PCR (Bio-Rad, Hercules, CA, USA). The level of target gene expression was normalized against ATCB expression. The mRNA fold change was calculated using the 2−ΔΔCt method, with the values expressed as fold change relative to the untreated control.
Insulin measurement by ELISA
The 1.1B4 cell was treated with FAC alone, cytokine (IL-1β or TNF-α alone), two cytokines (IL-1β + TNF-α), FAC combined with each cytokine (FAC + IL-1β or TNF-α), and FAC combined with both cytokines (FAC + IL-1β + TNF-α) for 24 h. Then, insulin secretion was assayed by ELISA using the human insulin ELISA kit (Merck, Kenilworth, NJ, USA), according to the manufacturer's protocol.
Statistical analysis
The results were analyzed by one-way and two-way analysis of variance (ANOVA) and presented in mean ± SEM of three independent experiments. The difference in results was considered significant when p < 0.05.
FAC induce ROS production, reduce cell viability, and decrease insulin expression of pancreatic β-cell
The accumulated iron in β-cell islets might promote ROS levels and cell apoptosis leading to a decrease in insulin production. The cellular ROS levels and cytotoxic effects of iron were first examined in pancreatic beta cells. FAC (0.1–15 mM) increased ROS levels in a dose-dependent manner (p < 0.05) (Fig. 1A). In addition, FAC induced pancreatic cell apoptosis and reduced cell viability in a dose- and time-dependent manner (Figs. 1B–1C and 1G). The highest ROS (MFI 1,375 ± 64.26) and apoptosis (40%) were found in 15 mM FAC-treated cells at 24 h (Figs. 1A and 1B). Expression of apoptotic regulatory proteins include BAX, BCL2 and STAT1 were assessed to study the effect of FAC on β-cell apoptosis. In the 1.1B4 cells treated with 8 mM FAC the BAX were up-regulated at 12 h and down-regulated at 24 h while BCL2 was down-regulated at 12 and 24 h (Figs. 1D–1F). The lowest cell viability (40%, p < 0.001) was found in cells treated with 10 and 15 mM FAC for 24 h (Fig. 1G).
Insulin expression and secretion were assessed to study the effect of FAC on β-cell function. In the 1.1B4 cells treated with 8 mM FAC the insulin (INS) expression and insulin level decreased in a time-dependent manner (Figs. 1H and 1I).
Combination of FAC and cytokines induce ROS production, apoptosis, and reduces insulin expression of pancreatic β-cells
Cytokine activates the production of ROS and reduction of insulin secretion which lead to diabetes. The effect of FAC combined with cytokines on pancreatic β-cells was observed. Cells were treated with IL-1β alone, TNF-α alone, IL-1β combined with TNF-α, FAC alone, and FAC combined with IL-1β, FAC combined with TNF-α, and FAC combined with IL-1β and TNF-α for 24 h. The results showed that ROS and percentage of apoptosis increased but had not significantly different compared between FAC with cytokines and FAC alone (Figs. 2A and 2B). Insulin secretion and INS expression was reduced in FAC combined with IL-1β or TNF-α compared with FAC alone (Figs. 2C and 2D).
The effect of 22.2 mM high glucose was also investigated in 1.1B4 cells, cell viability of pancreatic cell treated with cytokines with FAC was lower than in a normal glucose condition. The lowest cell viability was found in FAC combined with IL-1β and TNF-α in a high-glucose condition (Fig. 3A). In addition, the highest ROS was found in FAC combined with IL-1β and TNF-α in the high-glucose condition (Fig. 3B). Apoptosis of pancreatic cells in the high-glucose condition was higher than in the normal glucose condition. Interestingly, the highest apoptosis (Fig. 3C), lowest insulin secretion (Fig. 3D) and lowest INS expression were found in FAC combined with IL-1β and TNF-α in the high-glucose condition (Fig. 3E).
FAC combined with IL-1β and TNF-α decrease secretory function genes through antioxidant defense, apoptosis, and ER stress
From these results showed that iron (FAC) and cytokines reduce insulin secretion capacity, this evidence was examined by measuring the expression of genes involved in antioxidant defense, apoptosis pathways, ER stress, and secretory function (Fig. 4). Two important antioxidant genes in the pancreas, including superoxide dismutase 2 (SOD2) and glutathione peroxidase 1 (GPX.1) were measured. The results showed that the highest SOD2 expression was found in FAC combined with TNF-α alone and FAC combined with IL-1β and TNF-α (Fig. 4A). In addition, apoptosis-related genes, including B-cell lymphoma 2 (BCL2), Nuclear factor-κB (NFκB), and signal transducer and activator of transcription 1 (STAT1) were studied. The results showed that the expression of BCL2, NFκB, and STAT1 was decreased (Figs. 4C–4E). Gene expressions of Heat Shock Protein Family A (Hsp70) Member 4 (HSPA4) and Heat Shock Protein Family A (Hsp70) Member 5 (HSPSA5), ER stress marker genes, were analyzed. The highest gene expression of HSPA4 was shown in FAC combined with both cytokines. However, the gene expression of HSPA5 was decreased (Figs. 4F and 4G). Moreover, insulin-secretion related genes including glucose kinase (GCK), proprotein convertase subtilisin/kexin type 1 (PCSK1), and proprotein convertase subtilisin/kexin type 2 (PCSK2) were determined. The expressions of these genes were decreased more than two times in FAC treated alone and in combination with cytokines (Figs. 4H–4J).
DM is commonly found in β-thalassemia patients. The overload of iron and increased of cytokines may affect the β-cell function and insulin secretion capacity, which lead to DM (Wanachiwanawin et al., 1999). However, the mechanism of DM in thalassemia is still unclear. The purpose of this research is to study the effect of FAC combined with cytokines IL-1β and TNF-α on 1.1B4 in the human pancreatic islet cell line.
Firstly, we found that iron exposure resulted in a reduction of cell viability, induction of ROS, and increasing of apoptosis in a dose-dependent manner in 1.1B4 cells that have been previously reported in other types of cell line such as HH4, human hematopoietic cells/mesenchymal cells, MC3T3-E1 cells, and MG-63 cells through different mechanisms (Li et al., 2016; Lu et al., 2013; Doyard et al., 2012; Yang et al., 2017; Ke et al., 2017). Many reports have been demonstrated that iron overload lead to increasing intracellular ROS and caused cell damage, which is concomitant with our results. Moreover, our results showed the reduction of INS expression and insulin secretion that might be affected by ROS induced by FAC, which finally cause β-cell damage. FAC was previously used to induce the excess intracellular iron and caused increasing of ROS affected insulin secretion in Rat insulinoma pancreatic β-cells (RINm5F) as a model of pancreatic iron overload in β-thalassemia patients (Koonyosying et al., 2019).
The toxic effects of cytokines was studied but the mechanism or pathway underlying those effects in pancreatic β-cell is still unclear. Numerous studies whose findings reported that short-term treatment with cytokines such as IL-1β, TNF-α, and interferon γ (IFN-γ), alone or in combination could induce apoptosis of islet cells and dysfunction of pancreatic β-cells to release insulin (Vasu et al., 2014; Wang, Guan & Yang, 2010), are concomitant with our results.
Interestingly, β-thalassemia patients with iron overloading had high levels of these cytokines and high percentage of apoptosis. The iron and cytokines-induced apoptosis might be the cause of β-cell dysfunction and DM development in these patients. From our results, FAC combined with IL-1β and TNF-α had an additive effect on reducing INS expression, inducing SOD2 antioxidants' genes expression and activating endoplasmic reticulum (ER) stress. The increasing of ROS in FAC combined with IL-1β and TNF-α induced the expression of antioxidant SOD2 have been reported (Wang et al., 2018). In addition, the induction of SOD2 expression by cytokines was reported (Vasu et al., 2014). Moreover, the result revealed that the HSPA4 heat shock 70 kDa protein 4 genes responded to the activation of oxidative stress, protein-folding, and secretion demands that can be found in hyperglycemia (Berchtold et al., 2016). Jacob and co-workers found that 100 pg/ml of IL-1β induced divalent metal transporter 1 (DMT1) expression and caused increasing of ROS and activating apoptosis pathway in pancreatic islets cells (Hansen et al., 2012). This information is not consistent with our results that ROS and apoptosis in FAC combined with IL-1β and/or TNF-α were not significantly different from FAC treated alone.
Iron-induced ROS can activate ferroptosis which might be the possible mechanism of pancreatic β-cell damage in our study. It is a non-apoptosis cell death is induced by the small molecule or condition that inhibits glutathione biosynthesis or the cellular glutathione-dependent antioxidant enzyme glutathione peroxidase 4 (GPX4) and results in an accumulation of ROS (Cao & Dixon, 2016; Xie et al., 2016).
Experiments conducted with high glucose concentrations shows significant apoptotic death compared to normal medium in untreated cells however no change in cell count and ROS, because high glucose could activate apoptosis signaling pathway and down-regulation of anti-apoptotic proteins BCL2, MAPK8, and MAPK10 (Vasu et al., 2013). However, only high glucose had less affect to cell count and level of ROS but when combine high glucose with FAC, and cytokines could lead to increase level of ROS. Hyperglycemic condition in the combination of FAC and cytokines induce production of ROS and lead to apoptosis and cause reducing of insulin in 1.1B4 cells that might be resulting from the down regulation of INS and other genes involved in insulin secretion, including GCK, PSCK1, and PSCK2 (Vasu et al., 2014; Kooptiwut et al., 2005; Jonas et al., 2009; Laybutt et al., 2002). This might be due to the interference of high glucose that reduces the PDX1, an important gene involved in the secretory function (Sachdeva et al., 2009). The effect of cytokines on insulin production in hyperglycemia was found (Zhang & Kim, 1995; Kiely et al., 2007). Moreover, it was found that glucose toxicity induced ROS production and activated the apoptosis signaling pathway (Robertson, 2004). This information is concomitant with our study that the highest ROS, the lowest INS expression, and insulin secretion were found in FAC combined with IL-1β and TNF-α in high glucose level. This results could be supported the mechanism of insulin dependent or DM development in β-thalassemia patients who had iron overload and high level of cytokines IL-1β or TNF-α.
However, this study was performed only in human pancreatic β-cell line as a model which no direct measurement in humans and external validation but our previous data suggests that in β-thalassemia/HbE patients with diabetes had high level of ROS, low level of glutathione, reduced the β-cell function (HOMAB) and insulin level decreased (P. Rattanaporn, 2019, unpublished data).
This study suggests that FAC combined with cytokines, IL-1β and TNF-α in high glucose condition could increase ROS production, ER stress and apoptosis induction. However, they reduce insulin secretion in human pancreatic β-cell lines. This information could be benefit for understanding the mechanism on development of DM in thalassemia patient.


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Amitabh Bachchan has a short while ago shared a throwback picture on his Instagram tackle that dates again to 1985. Check out it out.
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Swetlana Neog
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Up to date: June 30, 2020 05:16 am
Abhishek Bachchan has an epic response post looking at father Amitabh Bachchan's portrait in these throwback Pics
Amitabh Bachchan is generally acknowledged for sharing humorous and satirical posts on social media. In fact, Major B who enjoys a large enthusiast subsequent on social media hardly ever leaves a probability to entertain his followers through the medium of his myriad posts. The megastar generally shares some of his great outdated memories on social media far too therefore cherishing the bygone periods. As we speak of this, he has shared a collage of previous shots from Paris on his Instagram take care of.
As described by Massive B himself, these photographs day again to 1985 when he frequented Paris with his family members. As we can see, he is finding his portrait manufactured by an artist even though Abhishek Bachchan stands close by and observes the similar. However, the latter's response is rather epic upon viewing his dad's portrait as is apparent in the photo. Amitabh Bachchan has also included a extended take note along with the publish that reads, "Paris, Montmartre, 1985 .. the renowned bohemian courtyard of artists and painters .. Created popular by the famous artist Toulouse Lautrec.. a film built on him 'Moulin Rouge' that starred the remarkable actor José Ferrer .. and later on Moulin Rouge made by Baz Luhrmann .. and of course "Calcuttaites" would dwell on the popular night time club Moulin Rouge, on Prak Road .. our nightly hold out for the duration of 1962 – 1968 .. and the band of Louis Banking companies and singer Pam Crane .. aaahhhh .. those had been the times my buddy .. Again to the photo… Abhishek contemplating that my portrait remaining drawn is likely all improper."
Test out Massive B's Instagram submit below:
On the work front, Amitabh Bachchan was very last observed in the film Gulabo Sitabo co-starring Ayushmann Khurrana that was released a few times back again. The audience cherished his effectiveness as the grumpy and hilarious Mirza in the film. He now has some appealing jobs lined up that involve Brahmastra, Jhund, and Chehre. Speaking about Abhishek Bachchan, he also has a several videos in the pipeline that include things like Ludo, The Significant Bull, and Bob Biswas.
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RE/MAX Plus
Alan Wood
2171 Monroe Avenue, Rochester, NY 14618
24 Vista View DrivePerinton, NY 14450
Key Details
Sold Price $142,250
Property Type Townhouse
Sub Type Townhouse
Listing Status Sold
Square Footage 1,182 sqft
Price per Sqft $120
Lot Size 1,306 Sqft
Year Built 1984
MLS Listing ID R1237952
HOA Fees $230/mo
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Subdivision Stillmeadow Courts
Bedrooms 2
Full Baths 1
Half Baths 1
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The wait is over!! Clean and pristine... Rarely available town house in Perinton is ready to make your own! Budget friendly Fairport Electric is easy on the wallet! Gleaming hardwood floors. Open kitchen to dining are with vaulted ceiling - loft over look. Two generous sized bedrooms upstairs. Open loft area with skylight - ideal office space. Full bath recently updated. Partially finished space in basement. Laundry in basement and storage area. All appliances remain. 1 car garage. Central air. HOA $230/month includes common area maintenance, exterior maintenance, master insurance, reserve fund, snow removal (including driveway), garbage & water. Open Saturday November 16th 12-1:30pm. Won't last long!
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Flooring Carpet,Hardwood,Varies,Vinyl
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Thank you Anne - that's very reassuring. I was a bit concerned when this came out of the blue! Can I just ask whether you ever get the visual effects without a headache?
Posted , 3 users are following.
Sorry to hijack this forum with a non-vestibular problem, but I suspect there's more expertise here than on the migraine forum.
I'm a 73-year-old female, good general health, fairly active, good lifestyle, BMI 24, BP borderline-high but monitored by my GP.
I have no history whatever of classic migraines - i.e. the type with an aura.
This afternoon, while preparing an email, I suddenly noticed a small scotoma in the centre of my visual field, whichever eye I looked through. Not so much a blind spot, more a tiny grey circular spot which it was difficult to see through. It covered about 2mm of text on my screen.
I downloaded an Amsler grid and tested my eyes. Left eye normal, but on the right eye the little blurry spot obscured a couple of mm of the grid just to the right of the central spot. I quit the computer, went off to do something else, to change the focal length.
The grey blurry central spot got bigger over the next 5 minutes - affecting both eyes equally. Then it started changing into a cluster of jagged black lines which appeared to be rotating, still in the centre of my visual field and getting rapidly bigger. It was partly obscuring my central vision though I could kind of see through it.
Shortly afterwards the cluster of jagged lines assumed a half-moon shape and started slowly moving off to the right of centre. It eventually formed a complete semi-circle around the right of my visual field, and looked as if it was rippling, like water. As it moved over to the right, I regained normal central vision but started losing vision in the right field. All this continued to affect both eyes.
It eventually disappeared off the right-hand edge of my visual field, leaving me with normal (for me) vision. The entire episode lasted about 40 minutes.
I had no other symptoms during this episode, apart from feeling a bit panicky. (It was the first time anything like this had ever happened to me.) I grinned at myself in the mirror a couple of times, to check for facial weakness, but there was none.
I checked my sight on the Amsler grid again after the end of the attack - everything OK. This happened five hours ago and I haven't developed a headache or any other symptoms since then.
Would this be a visual migraine? And is it normal for these to start so late in life with no antecedents?
0 likes, 8 replies
Just to add that these weren't the classic "floaters" of old age. I get these occasionally and know what they look like. Also my eyes were fully tested seven months ago, including retinal photography, and all was fine.
Thank you Anne - that's very reassuring. I was a bit concerned when this came out of the blue! Can I just ask whether you ever get the visual effects without a headache?
Hi Lily, yes my vision goes wonky as i I am unable to focus my eyes properly, everything looks blurred and my perception of distance is affected.I don't get a headache which is why they are called 'silent' migraines but sometimes I do get a thumping/pounding sensation in my ears.Overall I am much improved but stil have bouts of imbalance especially going down stairs and into light and dark areas. When it was at its worst I was crawling around on the floor as couldn't stand up. But at that time I also was suffering with BPPV and the two combined to make things worse. At the moment I don't have BPPV but I was having recurring bouts of it every few weeks and the epley manouvre didn't work for me. Eventually the crystals within the ear canal dissolve on their own and for me that can take about 6 weeks. It is my left ear that is affected with BPPV and could be due to a head injury on that side many years ago I was told by ENT
Sorry to hear that your BPPV keeps coming back. I was lucky with mine this last time round, as it was fixed in minutes with the Epley manoeuvre after about two months. That was four months ago and I've been fine since then. I was less fortunate the first time I got it, 25 years ago, when there was less knowledge about the Epley. As far as I recall, it took about a year for the crystals to dissolve spontaneously.
It sounds as if you have some other vestibular problem going on as well. I really feel for anyone who has permanent vertigo, having had the two BPPV experiences.
Fortunately I don't have any eye problems - apart from yesterday's incident plus dry eyes due to an autoimmune condition. I didn't have any focussing problems yesterday. What I experienced fits exactly with the description of scintillating scotoma. My heart was certainly pounding during the episode, but that was only because I was initially a bit freaked out by it!
I feel completely reassured now but I still think I'll touch base with my regular GP when he gets back from his holidays at the end of this month.
I hope your condition continues to improve.
If,you googe Moorfields NHS uk,,eye,conditons there's a,whole,bunch of different ones to click on which will describe symptoms. They are in London Uk and very reknowned. You can get Migraine Associated Vertigo with no headache , but as you dont mention dizziness of any kind i doubt you have that per se.
Thank you Gillian. No, I don't believe it's vestibular in origin either. I only posted here because I suspected I might get more information than over on the migraine site (which has proved to be the case so far).
I did, of course, have the two-month attack of BPPV that I posted about on here earlier this year, but I know there's no connection as the BPPV was completely cured by an Epley manoeuvre 4 months ago.
I've now done a bit more research and have discovered that it's not all that rare for these visual migraines to start out of the blue in old age, and that in the elderly visual migraines are more likely to occur without headache than in young people. However, I see there's a possible link with TIAs, so I think I'll still touch base with my GP when he gets back from his hols at the end of this month. There's a dodgy locum standing in at the moment, so I won't bother with him.
I've also withdrawn €50 (£40) in cash, which I'll keep handy in case I get a worse attack, or any worrying neurological symptoms. That'll pay the taxi to A&E at a very good university hospital on the other side of town if necessary. Under our health service we can present at any hospital or doctor we want, anywhere in the country - no catchment areas. But if you call an ambulance they have to take you to the nearest suitable place, and I don't want to get carted off to either of my flea-pit local hospitals!
And how are you? How are you getting on with the exercises now?
We want the forums to be a useful resource for our users but it is important to remember that the forums are not moderated or reviewed by doctors and so you should not rely on opinions or advice given by other users in respect of any healthcare matters. Always speak to your doctor before acting and in cases of emergency seek appropriate medical assistance immediately. Use of the forums is subject to our Terms of Use and Privacy Policy and steps will be taken to remove posts identified as being in breach of those terms.


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Hagi weather in July 2020
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- 2828°C max day temperature
- 1717 days with some rainfall
- 2222°C min night temperature
- 1414 hours of daylight per day
- HHigh heat & humidity
- 285285 mm of monthly rainfall
- 1111 (Extreme) UV index
- 2424°C sea temperature
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The July weather guide for Hagi, Japan shows long term weather averages processed from data supplied by CRU (University of East Anglia), the Met Office & the Netherlands Meteorological Institute. Find out more about our data sources.
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How hot is it in Hagi in July?
Daytime temperatures usually reach 28°C in Hagi in July with high heat and humidity, falling to 22°C at night.
How sunny is it in Hagi in July?
There are normally 6 hours of bright sunshine each day in Hagi in July - that's 39% of daylight hours.
How warm is the sea around Hagi in July?
The average sea temperature around Hagi in July is 24°C.
Does it rain in Hagi in July?
There are usually 17 days with some rain in Hagi in July and the average monthly rainfall is 285mm.
What is the chance of a typhoon in Hagi in July?
On average 1 day in July in Hagi is likely to be affected by the passage of one or more tropical storms.
Hagi sunrise & sunset times for July 2020
Browse the sunrise & sunset times for Hagi in July 2020. Select a month to view Hagi sunrise & sunset times for the next twelve months.
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Having Pets For Autistic Children, Is This Advisable?
08th December 2009
Bonita Darula
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Most children love animals and want to have pets. This includes children with autism. Pets can be a wonderful addition to the family, but it can create problems and sometimes be harder on the pet than the family members. Is it advisable?
Before thinking about purchasing a pet for a family with an autistic child, it is advisable to take into consideration what kind of pet does the family want in their home. For example, is it going to be a dog, cat, bird, hamsters, what exactly is the pet going to be? In addition, the family should discuss the care of the pet, the cost, is it an indoor pet, an outdoor pet, who will have the responsibility to take care of the pet. Will it be a family joint effort, where all the family members will take turns and share the load of taking care of the pet, including the child with autism.
The individual who has autism in the family, may love the pet, but may not be able to take responsibility of caring for the pet. In other words, take the pet for a walk, if it is a dog, feed the pet, clean up the debris of the pet and litter that is accumulated, allowing the animal inside the house or outside the house for exercise and fresh air.
There are certain pets that require more care and attention than others do. This is true compared to having a cat as a pet verses a dog as a pet. Dogs usually require more attention, and care than cats do. It is a good idea to take into consideration, who is going to be home during the day with the pet. In addition, what about traveling or taking a vacation, will the pet be able to travel with the family, or will you have to hire someone? Will the pet adjust to traveling or the care of someone else?
Pets in a family with autism can be very therapeutic and can encourage the autistic child to have responsibility, follow instructions, love the pet with hugs, petting it, talking to it, crying and expressing emotions to the pet, that they may not do with other family members. It is imperative to realize, some autistic children display outbursts of anger and tantrums. Because of this kind of behavior in autistic children, you must remember that having a pet in the home may be a target for this kind of behavior. It is wise, not only to think of the family having a pet with a child that has autism, but take into consideration how the pet will adjust and be affected by the child if he or she has outbursts of anger or tantrums. The pet should never be in any danger with the child present if this occurs.
Take your child and family members to various pet stores, visit other families who own and take care of pets, to evaluate if your child with autism responds to the various kinds of pets in a positive way. Purchase a stuffed animal that looks or is the same kind of pet you and your family want as part of the home. Take the stuffed animal and practice and show the child what to expect when the animal comes home as a permanent pet. For example, if you decide to purchase a dog, buy a small leash, some dishes for the dog food, show the child where the dog is going to sleep, practice taking the stuffed animal for a walk with the leash, practice having the stuffed pet drink water and eat food out the dishes, let the child know, dogs bark, growl, cats meow, sometimes claw furniture, climb and jump. Make it a fun part of the child with autism so he or she becomes aware of what to expect if an animal as a pet, is brought home.
"Bonita Darula, is known world-wide, for her knowledge and insight on autism. Visit===
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Former Minister of Labour, Alpha Timbo, Deputy Minister of Basic and Senior Secondary Education, Emily Kadiatu Gogra, Permanent Secretary, Ministry of Basic Secondary School Education(MBSSE), Charles Tom Kamanda, Director of Nutrition, School Feeding, MBSSE, Mamusu Massaquoiu Zainab, and Binta Kamara, a businesswoman at Lion Inn, will be tried by Judge and Jury.
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Hot Springs Village resident Mike Anderson was born in Minnesota, but moved around a lot because his father was career U.S. Air Force. Georgia, Florida and Texas were just some of the places the Andersons lived as he grew up.
After high school graduation, Anderson enlisted in the Army in September 1968, first going to Fort Bliss, Texas, for basic training. He still remembers getting off the bus and a drill sergeant asking if anyone weighed over 200 pounds. Being a military "brat," he knew better than to raise his hand, but others did raise their hands. "The drill instructor said, 'Okay, you guys are in fat man's platoon'," Anderson said.
Anderson told me that basic training was good for him, made him grow up and helped him to meet people.
The training involved trips to the rifle range, crawling under barbed wire, long hikes and going through the dreaded gas chamber.
Anderson had signed up with the Army to be a mechanic and after completing boot camp he got his orders. He was being sent to Germany to join the 24th Infantry Division in Augsburg as a 63B20 – a wheel vehicle mechanic.
Soon after arriving in Germany, he was changed to a motor pool generator mechanic, something he had never done before. "They (the generators) were all lined up. None of them worked and all I had was books to look at," he said of the unexpected job. But one by one, big and small, he got all of them working as good as new.
Anderson said Germany was beautiful country. The castles, mountains and the people were great.
His typical day started early with a cup of coffee, then he was off to the motor pool to work on generators and large trucks, fixing brakes or whatever else was necessary to get them rolling again.
In June 1970 Anderson received new orders. He was going to Vietnam. After a leave back home to Minnesota he received notification of receiving a security clearance. Anderson was assigned to the 525th Military Intelligence Group in Saigon as a motor pool mechanic.
From Seattle he flew to Cam Ranh Bay. I asked what his first impressions were. "I was scared. I landed in Cam Ranh Bay and then got the orders for Saigon," he said, adding Vietnam was really hot and sticky.
As for Saigon, Anderson said he only made a few trips to the city and found it to be dirty, with people and bicycles everywhere. Did you feel secure in Saigon, I asked. "Yes and no. You always heard there was stuff going on there. I felt safer being there with a few buddies," Anderson said.
A day of work with the 525th included working on jeeps, trucks, carburetors, motors and whatever else was needed to make the equipment usable. The motor pool was large, wide open and had some bunkers and fences.
He said there were no attacks while he was there, but there were helicopters coming in with dead bodies, day and night. This really bothered him as time went on.
Anderson said it bothered others, too, including one man who killed himself with an M-16. "You could see on the sides of the helicopters, body bags. That bothered me a lot," said Anderson, who added this interview was the first time he'd talked much about this. "I haven't told this stuff to my wife, either," he said.
About this time the 525th was attached to the First Infantry Division, Third Brigade. Anderson was still working in the motor pool just outside Ton Son Nhut doing the same work, except for a time when he was sent to a helicopter outfit. But just a few days later he returned to the 525th .
In May 1971, Anderson returned home. He landed in California, was approached about re-enlisting, but declined the offers.
From California he flew home to Minnesota and talked to me about that experience.
Chants of lady-killer, kid-killer and being spit at was his welcome home at the Minneapolis airport.
Anderson said he asked himself why he was wearing his uniform and was shocked at how his countrymen were treating him. I asked if he had any idea that such treatment was awaiting him on U.S. soil. "I just couldn't believe it. No, I had no idea what I was going to see," Anderson said, then spoke highly of how servicemen are treated now.
Anderson was discharged on May 14, 1971. As a civilian he attended Pine City Vocational School in Pine City, Minn., graduated and worked as an auto mechanic in the Twin Cities.
Later he went into the food industry as a gourmet food distributor. Thirty-five years later he retired after time spent in several positions such as order filler, truck driver and warehouse supervisor.
Anderson retired in January 2012, and with wife Judy moved to the Village in July 2014. They have two daughters, Michelle and Carrie.
Reflecting back on his days in uniform, Anderson said it made him grow up fast. "It made me a man, I can tell you that," he said. Anderson noted that he was glad he made it back home and it made him look forward to the future.
Anderson served with U.S. Army in Germany and Vietnam
Hot Springs Village resident Mike Anderson was born in Minnesota, but moved around a lot because his father was career U.S. Air Force. Georgia, Florida and Texas were just some of the places the Andersons lived as he grew up.


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Recruitment Agencies in Kidderminster
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