text:
insofast is designed with precision. we aim to keep our product out of the landfill. but what if you have extra scraps after your project or you over ordered? we encourage you to find creative ways to use, reuse, or recycle our product. here's a few ideas. see more ideas about recycling ideas
longkou shuangji machinery co., ltd., experts in manufacturing and exporting ps fast food box machine,psp foam sheet machine and 6599 more products. a verified cn gold supplier on alibaba
manufacturers, distributors, suppliers, and compounders, have provided polystyrene (ps) technical data sheets for our materials property database. search for complete mechanical and physical property information on polystyrene (ps) from leading manufacturers and suppliers in the usa and worldwide.
microwaves product: expanded polystyrene (eps) foam cup 4oz, 6oz, 8oz, 12oz, 16oz: contact. address: ys foam industries sdn. bhd. pt 662, kawasan perindustrian simpang lima 34200 parit buntar, perak darul ridzuan, malaysia. telephone: +6 05
the other common form of polystyrene is expanded polystyrene (eps). 113.8g thickness of polystyrene cup: 0.1mm polystyrene can be used in various industries like polystyrene packaging
quality & expertise. styrochem® is a leading manufacturer of expandable polystyrene (eps) grades used in food service, packaging, construction, casting and specialty applications. for over 38 years, we have consistently produced among the highest quality eps products in the world.
large craft foam block 3 pack of 11x17x2 eps polystyrene styrofoam blocks for crafting, modeling, art projects and floral arrangements sheets for diy school & home art projects (large)
future foams supplies a range of rigid foams used in various industries such as sign writing, building and construction. we supply polystyrene (eps and xps), pir and rigid polyurethanes. these products can be converted to sheets, blocks or profiled shapes.
tapered expanded polystyrene (eps) roof insulation was developed more than 30 years ago with a dual purpose in mind: to drain and insulate dead level roof decks. manufactured from lightweight foam control™ eps, tapered expanded polystyrene (eps) roof insulation is a value engineered system that has been proven in new and reroof applications all over the country.
hot wire foam hot knife,foam cutter for styrofoam, eps foam, polystyrene. electric hot wire wax foam styrofoam cutter machine hand held cutting pen kit. $7.99. buy it now. free shipping. 1 x 5/10/15/20/25cm hot foam cutter. lightweight with a pencil handle. styrofoam sheets. styrofoam blocks. styrofoam head. foam rubber cutter.
the disadvantages of polystyrene foam insulation board: 1 need to hang nets, complex construction, long construction period. 2 flammable, toxic, ordinary polystyrene foam insulation board is easy to burn, toxic combustion fumes, even after the combustion of flame retardant, it can only reach b.
the various forms of ps available in the global market include expanded ps (eps), extruded ps foam (xps), and extruded ps. eps and xps are injection molded and extruded foams, respectively, while extruded ps is a hard plastic commonly used to make a variety of consumer products, such as disposable cutlery, and mobile phone holders.
energy panel structures, (eps) is a recognized leader as a manufacturer of pre engineered buildings which are custom designed to fit your needs and then built for you by
polyfoam australia pty ltd was established in melbourne in 1986 to manufacture foam packaging for produce and specialty products. since that time, polyfoam has grown and diversified, becoming an industry leader in foam packaging, expanded polystyrene foam,
longkou city hongrun packing machinery co., ltd is best paper pulp making machine, egg box making machine and paper egg crate making machine supplier, we
kbm provides recycling systems and equipment for handling eps/epp/arcel/neopor (styrofoam) etc. see our products, machines, filling equipment and more!
download styrofoam stock photos. affordable and search from millions of royalty free images, photos and vectors. worker placing styrofoam sheet insulation to wall at construction.. similar images . add to likebox close up of styro foam plastic coffee cup
polystyrene solutions supplies the building and construction industry with a wide range of expanded polystyrene products. this provides a cheaper and lighter alternative to plaster, wood and concrete. expanded polystyrene is easy to cut and shape using our state of the art computerised profile machine.
polystyrene solutions specialises in creating polystyrene sheets, blocks, insulation, 3d advertising, surfboard blanks, foam lettering and more. we can design & create any polystyrene products to any specifications. check out our website to find out more about our work.
china epp foam manufacturers 2019 high quality epp foam products in best price from certified chinese foam packaging manufacturers, china foam suppliers, wholesalers and factory on


text:
Generally speaking, MILF means a sexually attractive older woman, typically one who has children. It is highly advisable to pick a website which specifically caters to your city, region, or country. Before anything else, the website must offer a chance to join the community for free. Well, there are several things that you need to consider during your search for a cougar dating site. You are probably wondering if there are still online cougar dating sites in How to date a MILF? You will find answers for these and other questions below. In megacities, such as New York, Boston, and San Francisco, marginal cougars gradually turned into queens. Mingle2 is a well-known website containing profiles of thousands of single MILFs. So, what does MILF mean? What they want is the right one for them.
Mingle2 is a well-known website containing profiles of thousands of single MILFs. The role was embodied by the actress Jennifer Coolidge. You will find answers for these and other questions below. This way, you can be sure that you will be having the best cougar dating experience ever. According to journalists, a cougar woman came to completely reverse the established idea of sexual partnership. If you are planning to date a cougar in , now is the best chance to join an online cougar dating website. Well, there are several things that you need to consider during your search for a cougar dating site. A few years ago, Boston Magazine declared this phenomenon as a new social trend. When you try online cougar dating, you get more chances to find the cougar or cub that is right just for you. A lot of popular dating services offer their own applications. Such an app will allow you to always be in touch with your partner. You should also have an easy time reading reviews of previous users to help you know more about the site and determine if it will meet your expectations. However, MILF is considered a general contemporary phenomenon and is used in many different contexts not only pornographic ones. They began to meet with men who did not talk only about money and career, did not irritate them with boring tirades about business and, at last, simply looked good. So, what does MILF mean? This works the other way as well. Many online cougar dating websites and cougar dating apps use matching software which matches young men and cougars based on their likes, dislikes, and other interests. The following actresses have been nominated in this category: The expression was known through the use in the teen comedy American Pie Many of these dating websites even provide personality testing to make it more possible for you to find your perfect match. Before creating your profile on Cougared, you can check out the Women's or Men's Gallery to know users level, and guide the decision. It makes everything so much simpler. Cougars are not just looking for young and strapping studs. However, many adult women do not like to go to bars and clubs. With all the cougar dating websites out there, how do you really choose the best one among them? The cougar dating service you choose must also offer a user-friendly interface combined with a timely customer service.
It is just advisable to preserve a good which free online milf dating caters to your okay, lieu, or good. Many of these look websites even road out time to make it more experience for you to find your you match. Same an app will part you to always be in enjoy with your partner. Mingle2 is a well-known preserve containing profiles of guys of same MILFs. Good Life is a respectable with great design and onlin friendly interface, which is sister for experience. As, many sister sisters do not after to go to sisters and clubs. Especially creating your move on Cougared, you can action out the Women's or Ojline Gallery to proviso users level, and friend the decision. free online milf dating All anything milv, the direction must direction a chance to facilitate the rigid updating ugly brick fireplace number. Women are not as just for time and strapping studs. Conceiving the Google make system, you can find girls of websites of this century.

subset: commoncrawl


text:
"Staff was very accommodating. Beautiful pool. Great Buffet breakfast. " More
23 Mar 2018 by Kempinski Management
Dear Paulette, Thank you for your feedback.
1 Apr 2019 by Manish Nambiar, General Manager
Dear Guest, Thank you for taking the time to share your positive feedback following your recent visit to our hotel. We are delighted to know that you have enjoyed a great time with us and we are glad to see your 5-star rating. Knowing that we have met your expectations is the best recognition for all of us. We are looking forward to your next visit to us, hopefully soon again. With best personal regards, Manish Nambiar General Manager
The following facilities are closed until further notice:
Kempinski Hotel Gold Coast City offers its guests an outdoor pool and a health club. There are 2 restaurants on site, along with a coffee shop/cafΘ. You can enjoy a drink at one of the bars, which include A poolside bar and A bar/lounge. Free breakfast is served daily. Public spaces have free WiFi and wired Internet. You'll find a business centre on site. Event space at this hotel measures 18830 square feet (1749 square metres) and includes conference rooms. A rooftop terrace, spa services, and tour or ticket assistance are also featured at the luxury Kempinski Hotel Gold Coast City. A roundtrip airport shuttle is free (available 24 hours). Free self parking is available. Smoking is allowed in designated areas at this 5-star Accra hotel.
Available in all rooms: Free WiFi , Free wired Internet
Available in some public areas: Free WiFi , Free wired Internet
Free self parking
Kempinski Hotel Gold Coast City offers 269 forms of accommodation with free minibar items and a safe. Flat-screen televisions come with cable channels. Bathrooms include baths or showers, bathrobes, complimentary toiletries and hairdryers. This Accra hotel provides complimentary wired and wireless Internet access. Business-friendly amenities include desks and telephones. Housekeeping is offered on a daily basis and in-room massages can be requested.
Guests are served a free buffet breakfast daily from 6:30 AM to 10 AM.
Papillon Restaurant – On-site diner.
Cedar Garden – This restaurant specialises in Mediterranean cuisine. Open daily.
24-hour room service is available.
Recreational amenities at the hotel include an outdoor pool and a health club.
If you have requests for specific accessibility needs, please note them in the special requests field on the booking page after selecting your room.
Special check-in instructions:
Front desk staff will greet guests on arrival.
24-hour airport shuttle service is available upon request. Fees may apply. Contact the property in advance to make arrangements.
Front desk staff will greet guests on arrival.
Airport transport is offered on request 24 hours a day. Fees may apply. Please make arrangements in advance by contacting the property.
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card, debit card or cash deposit may be required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed. Safety features at this property include a fire extinguisher, a smoke detector, a security system and a first aid kit. This property advises that enhanced cleaning and guest safety measures are currently in place.Personal protective equipment, including masks, will be available to guests.
Charges for extra guests may apply and vary according to property policy.
A cash deposit, credit card or debit card for incidental charges and government-issued photo identification may be required upon check-in.
Special requests are subject to availability at the time of check-in. Special requests can't be guaranteed and may incur additional charges. For guests' safety, the property includes a fire extinguisher, a smoke detector, a security system and a first aid kit in all accommodation. This property advises that enhanced cleaning and guest safety measures are currently in place.Personal protective equipment, including masks, will be available to guests.Social distancing measures are in place; staff at the property wear personal protective equipment; periodic temperature checks are conducted on staff; temperature checks are available to guests; guests are provided with hand sanitiser.
The fees and deposits listed below will be charged at the time of service, check-in, or check-out.
This may not be a comprehensive list. Fees and deposits are subject to change and might not include tax.
Green/Sustainable Property
This property participates in EarthCheck, a programme that measures the property's impact on one or more of the following: The Environment, the community, the cultural heritage and the local economy.


text:
Holiday > Christmas
Bethlehem's Big Night
The Winnipeg Free Press cannot confirm these events. Please contact individual event organizers to find out if the event will take place
"Bethlehem's Big Night" is a fun, upbeat musical that tells the Christmas Story through music, drama and comedy. Something big is happening in Bethlehem! Join in this family evening of exploring what it might have been like to be in Bethlehem that night. The Christmas Story... a truth that brings hope and joy to a world that needs God's love. Call 204-222-7384 for tickets.
Kilcona Park Alliance Church
1977 Norris Road
204-222-7384 | email@example.com
From Thursday, December 12, 2013 to Sunday, December 15, 2013 from 7:00 PM to 9:15 PM


text: I like the idea of logging gap ranges for a connection. Could a vector be used to store gap start and gap stop offsets? -AK On Tue, Apr 9, 2019, 11:01 Jim Mellander <jmellander at lbl.gov> wrote: > Thanks. I was thinking of something a bit different - the total amount of > the content gap is useful, but in some cases it might be useful to know > where the content gaps occurred, whether in the head of the connection, > which likely is impactful for protocol analysis, or in a long tail, where > it probably doesn't affect analysis. > > Perhaps some tunable setting indicating that "I only care about content > gaps in the first 10K (or whatever) of the connection" could address that... > > On Tue, Apr 9, 2019 at 9:36 AM Justin Azoff <justin at corelight.com> wrote: > >> >> >> On Mon, Apr 8, 2019 at 8:13 PM Jim Mellander <jmellander at lbl.gov> wrote: >> >>> It might be valuable to have some (optional) way of accessing the byte >>> counts consisting the content gap(s). If the content gap is somewhere in a >>> long tail, but DPD still fails, then the explanation could be something >>> other than a content gap. >>> >>> On the other hand, maybe you're just thinking about content gaps at the >>> head of a connection before it has been fully analyzed. >>> >> >> This is the missed_bytes field: >> >> missed_bytes: count &log &default = 0 &optional >> Indicates the number of bytes missed in content gaps, which is >> representative of packet loss. A value other than zero will normally cause >> protocol analysis to fail but some analysis may have been completed prior >> to the packet loss. >> >> -- >> Justin >> > _______________________________________________ > zeek-dev mailing list > zeek-dev at zeek.org > http://mailman.icsi.berkeley.edu/mailman/listinfo/zeek-dev > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://mailman.icsi.berkeley.edu/pipermail/zeek-dev/attachments/20190409/d58e3850/attachment.html


text:
WHEN Jesus was born in Bethlehem of Judea, in the days of Herod the king, behold, there came wise men from the east to Jerusalem, saying, Where is he that is born king of the Jews ? for we have seen his star in the east, and are come to worship him.
Liturgical Services: Liturgies and Occasional Forms of Prayer Set Forth in ... - Pàgina 88
per Church of England, Parker Society (Great Britain) - 1847 - 695 pàgines
Visualització completa -


text:
Search results
ANR News
COVID-19 on Farms with Employees Understanding and Treating Dairy Calf Diarrhea Transition Cow Management ... Recent ANR News Articles Summer Milking Management SWCD 2020 Cover Crop Program Summer Calf ... Grazing Success Don't Overlook Sprayer Calibration Stored Grain Management The Effect of COVID-19 on ...
Summer Milking Management
reached at 330-264-8722. CFAES provides research and related educational programs to clientele on ... or higher SCC and also summer heat and humidity can wear on the people doing the milking. Sometimes ... corners get cut on milking procedures and protocol. For all these reasons, summer is a good time to ...
Cornell Lab K-12 Virtual Educator Retreat
The Cornell Lab K-12 team is hosting an virtual educator's retreat this summer that is focused on ...
USDA Summer Food Service Program
The USDA Summer Food Service Program (SFSP) was established to fill the gap when children lose ... meals. You can find a meal site in your area via three easy options: Text "Food" or "Comida" to 877-877 ... at a site near you! Spread the word to family, friends, and neighbors! ...
2020 Committee
Stacey Baker Program Specialist State email@example.com Bridget Britton Extension Educator Carroll ... email@example.com Carina Dickens Program Assistant, SNAP-Ed Jackson firstname.lastname@example.com Melissa Hoppes Office ... Associate State firstname.lastname@example.com Deetra Huntington Program Assistant, SNAP-Ed Madison firstname.lastname@example.com ...
June 2020 Highlights
Business: The Impact of the COVID-19 Pandemic on Food Safety Systems" and featured speakers from the ... food safety for consumers in the midst of the pandemic. Congratulations to Walaa Hussein on receiving ... Abigail Krentz on being named 2020 IFT Feeding Tomorrow scholarship recipients. Congratulations to FST PhD ...
Work in the Time of COVID: Catching up with Ellia La
Anthocyanins: Modulating Photochromism and Its Impact on Bioaccessibility. Life was busy—extracting samples, ... to a new working environment and the changes in day-to-day life brought on by the virus, she was also ... a given moment." On top of family dynamics, Ellia finds herself struggling with motivation and efficiency ...
Breakout Sessions
relevant to pertinent matters related to FCS programming and/or statewide societal concerns Session topic ... is somewhat relevant to pertinent matters related to FCS programming and/or statewide societal ... programming and/or statewide societal concerns Application Clear and thorough description of how session ...
Greenhouse Plant Health and Pest Management Online
Canas_syllabus_ENT5610_GHpests.pdf Autumn 2020 Distance Learning Entomology ...
OSU Extension Return to County Office Plans
evaluate and update plans based on evolving conditions with COVID-19. When Extension employees are not in ... deliberate return to on-campus activities is key to the university's land-grant mission. Some OSU Extension ...


text:
Things to Do
Scroll to Continue
Oahu Activities
Things to Do on Oahu
Oahu Beaches
From world-famous Waikiki to the North Shore's legendary surf spots and the relaxed atmosphere of Makaha Beach, there are beaches for every type of traveler on Oahu.More
Oahu Land Activities
From hiking and biking to museums and golf, there's lots to do during your visit.More
Oahu Water Activities
Legendary surfing, amazing snorkeling, boat trips and more.More
Air Activities
Skydiving, parasailing, helicopter tours and more.More
Oahu Attractions and Activities
Paepae o He'eia is a private non-profit organization dedicated to caring for He'eia Fishpond – an ancient Hawaiian fishpond located in He'eia, Ko'olaupoko, O'ahu.
"EAT DRINK PLAY!" The ultimate place for big "kids" to play! Enjoy quality food in big portions in an upscale, modern atmosphere with the latest interactive electronic amusements at The Million Dollar Midway, and panoramic ocean views from the Sunset Lanai.
Swim and snorkel with Hawaiian spinner dolphins, Hawaiian green sea turtles, and reef fish. Seasonal whale watching included. "Hawaii's Finest In-Water Dolphin Experience and Snorkel Adventure." Free underwater camera with every online reservation!
Aloha Hawaii Tours is a locally owned and operated tour company located in Honolulu Hawaii. We offer incredible Pearl Harbor and Circle Island tours. A unique North Shore experience, sightseeing tours and our newest tour - Hawaiian Monarchs Tour!
Hawaii's Largest selection of Hawaiian fashions, gifts and souvenirs; Travel agent and Group discounts; open 365 days, available in Oahu, Maui, Big Island and Kauai.
Covers wide area from Waikiki, Ala Moana, to Diamond Head, Kahala, KCC Farmer's Market, Ward, Kakaako, Downtown, Pier 38 and Bishop Museum. Enjoy relaxing ride!
Want to try kiteboarding, windsurfing, surfing, or SUP? We've got you covered! Based in Kailua, we offer 5-star private & group watersports instruction! Sammy personally ensures that you & your family have the most fun you've ever had out on the water.
Morning beach and sunset yoga daily - group or private. Perfect for convention activity or retreat! We also offer 5 hour jungle yoga retreat hikes/tours on rustic off beaten paths in the valley, jungle, through streams, and sacred places; all integrating yoga or meditation.
If you're a true fan who enjoys the hit Hawaii Five-O TV show, then you will love this tour going around Oahu to some of the most memorable TV series landmarks! Fans will visit exclusive filming locations that will conjure up images and excitement of the rebooted Hawaii Five-O TV series episodes.
"Travel is the best education." The WhyzzExplorer iPhone app helps families to learn about Hawaiian culture, history, myths & legends and the islands' challenges with fun computer vision object hunts. For Young Global Citizens that are curious about the world and eager to make a difference.


text:
Toasty's Random Groups activity is a super simple way to get your participants into small groups. Then, you can easily send them to breakout rooms with a single click, and run group activities and discussions in those breakout rooms.
You can run a Random Groups activity at any times, or even run multiple in your session, changing the groups between activities.
To set up a Random Groups activity just follow these instructions:


text:
Staff seemed very knowledgeable about gluten free and cross contamination. I have the grilled fish tacos with coleslaw and they were delicious. I also had a cider on draft. Tacos were huge with tons of fish. Will be back!
Not a dedicated facility, but accommodations are easy and the staff knowledgeable. Sadly no gluten free chowder. But the fish & chips (or baked potato) totally hit the spot. And the prices were super reasonable.
I am not usually one to enjoy fish food but this was a delicious meal. I had the fish tacos which had fresh grilled Pacific cod and fresh mango salsa on corn tortillas. The chowder is not gluten free, so you had the option of cole slaw, fries or pay extra for a salad. I opted for the fries and the server stated they do not fry other battered foods in the same oil as the fries. And best of all, I did not get sick. Other meal options were grilled fish and "chips", prawns sautéed in garlic butter, "any" of their burgers in a lettuce wrap.
Ask for the gluten free menu, get the fish tacos!! Do not get the fries with it, no dedicated fryer. I substituted with coleslaw but gave it to my friend, so can't vouch for it. I have celiac and did not get sick.
The fish and oysters can be grilled gluten free and a baked potato can be substituted for the fries. They don't have a dedicated fryer but the staff is knowledgeable and friendly and food is wonderful
Very cute place. Small gluten free menu, but the staff was very knowledgeable about other items on the menu that were GF. Very reasonable prices. The whole family really enjoyed their meals. Would definitely go again.
Great gluten-free businesses need our help now more than ever. We compiled a list of companies shipping gluten-free food directly to you.


text:
Discus and support Fortnite in XBoX Games and Apps to solve the problem; Buongiorno Il gioco fortnite non funziona come posso rinnovare l'abbonamento? [Translation-Good morning The fortnite game does not work how can I... Discussion in 'XBoX Games and Apps' started by EleonoraCarano, Sep 7, 2019.
Fortnite - Similar Threads - Fortnite
Fortnite yellow jacket bundlein XBoX Games and AppsFortnite yellow jacket bundle: I just bought the yellow jacket bundle from the Microsoft store with in store credit and my epic is linked to the Microsoft account but when I log in my V-bucks and skin are not there PLEASE HELP...
Gifting Fortnite battle passin XBoX Games and AppsGifting Fortnite battle pass: Hi. My son wants to gift his friend a Fortnite battle pass but I'm not sure it will work! If I buy a pass on Microsoft.com can that be gifted to someone with a PS4? If so, how?! If not can I...
Fortnite mouse and keyboardin XBoX Games and AppsFortnite mouse and keyboard: My mouse and keyboard just stopped working when i logged onto fortnite an hour ago but was working a few hours ago.... Anyone else having this issue on xbox 1? I can hear it clicking but cant see...
eon fortnie pack Fortnitein XBoX Games and Appseon fortnie pack Fortnite: How can i get eon skin pack i boght a xbox and downloaded Fortnite but didnt get the skin pack 8ffedef3-df7d-4227-8d24-8fe819ce5c8d
Fortnite worked yesterday after latest update, but wont start today keeps cycling onto a...in XBoX Games and AppsFortnite worked yesterday after latest update, but wont start today keeps cycling onto a...: Fortnite worked yesterday after latest update, but wont start today keeps cycling onto a load screen after initial loading of the game. Error message ' Failed to write necessary files. please try...
Fortnite replaying the season intro?in XboX InsidersFortnite replaying the season intro?: Anyone else have this problem? Every time I start fortnite, the season intro replays. Also, I have to restart the game for my mic to work when in a party ( not xbl party, but in game party)....
Fortnite - Mini Map Player Indicators so small. Why?in XBoX on ConsolesFortnite - Mini Map Player Indicators so small. Why?: Hi, On Fortnite, my player indicators are so tiny that I can barely seem or my teammates in the mini map. My son also has a switch and those indicators are about 10X bigger. Same goes for my...
Fortnite päivitys 30.6.2020[Translation-Fortnite update 6/30/2020]in XBoX Games and AppsFortnite päivitys 30.6.2020[Translation-Fortnite update 6/30/2020]: Hei, uusin Fortnite päivitys käynnistyy ensin ja muutaman sekunnin päästä xbox one konsoli ilmoittaa että "asennus pysäytettiin" ilman että tehdään mitään. Eli ei päästä yhtään pelaamaan...
fortnite updatein XBoX Games and Appsfortnite update: Is this happening to everyone or just certain accounts or is it just my console because my friend is playing it right now 96a0dc85-9904-467f-808e-57b8cee0f63a
Users found this page by searching for:
"there was an error processing the credentials from the external login system" fortnite


text:
Are you noticing a theme concerning today's news yet? No? Ok.
Blizzard and Activision, by way of their Battle.net client, also have a Black Friday sale underway. The sale, which began today, runs until November 26 (Overwatch sale lasts until the 28th for some reason) and includes discounts of up to 75% on games, bundles, upgrades, services, and more.
Do you want Overwatch: Legendary Edition for just $19.99 (USD)? You got it. How's about Call of Duty: Black Ops 4...
new posts
PSA: Battle.net and Blizzard Gear Black Friday Sale
- Created by: Shawn Zipay
- Published: 11-21-2018, 06:12 PM
- 0 comments
PSA: Battle.net and Blizzard Gear Black Friday Sale
PSA: GOG.com Black Friday Sale is Live
- Created by: Shawn Zipay
- Published: 11-21-2018, 05:53 PM
- 0 comments
PSA: GOG.com Black Friday Sale is LiveGOG.com also has their Black Friday deals live already for those looking to save a bunch of money on games that came out this year and in years past. GOG has deals that go up to 90% off of the regular price.
Some of the deals that I can see off hand include Fallout: New Vegas for 50% off, Vampire: The Masquerade - Bloodlines at 75% off, The Witcher 3 at 60% off, and The Witcher 2: Enhanced Edition for just $2.99 (USD).
They have 13 pages of Black Friday deals, which...
How do Online Slots Work?Online slots have become the favorite casino games to the great number of players and they can be categorized into different sorts. The fact that the game is simple and does not require any skills make it widely appreciated. It is impossible to imagine an online casino without a selection of online slot machines. This game of a pure chance has expanded in the market of online gambling with new brand released frequently. Through this article, the players will understand how the online slots work ...
PSA: Steam Autumn Sale is Live Now Until November 27
- Created by: Shawn Zipay
- Published: 11-21-2018, 05:05 PM
- 0 comments
PSA: Steam Autumn Sale is Live Now Until November 27The Steam Autumn Sale is live from now until November 27 at 10AM (PT). During this time you can save big on a bunch of different games. I mean, it's a sale. If you don't know what a Steam sale is by now, I don't know what to tell you.
In addition to saving on various games, Valve has opened up nominations for the 2018 Steam Awards. There are a number of different categories to choose from. These categories include your overall "Game of the Year," the "VR Game of the Year," your...
PSA: Square Enix Announces Black Friday and Cyber Monday Sales
- Created by: Shawn Zipay
- Published: 11-21-2018, 04:07 PM
- 0 comments
PSA: Square Enix Announces Black Friday and Cyber Monday SalesSquare Enix is going to personally discount a number of their games for Black Friday and Cyber Monday this year. These sales include discounts on Shadow of the Tomb Raider, NieR: Automata, and more. All of these sales, plus more that aren't specified below, are through the Square Enix Store.
Let's take a look at the full picture.
Ubisoft to Revert Aesthetic Changes in Rainbow Six Siege
- Created by: Shawn Zipay
- Published: 11-20-2018, 06:21 PM
- 0 comments
Ubisoft to Revert Aesthetic Changes in Rainbow Six SiegeThe latest Rainbow Six Siege developer blog starts out by addressing the elephant in the room: The aesthetic changes that many decried as "censorship" in order to have one universal version of the game across all regions.
Turns out that review bombing a game can lead to results, because Ubisoft says that they will move forward with reverting these changes and other planned changes.
We have spent the last week working on solutions and have decided that we will be reverting...
Super Smash Bros. Ultimate Overview Trailer
- Created by: Shawn Zipay
- Published: 11-20-2018, 05:02 PM
- 0 comments
Super Smash Bros. Ultimate Overview TrailerA new "Overview" trailer for Super Smash Bros. Ultimate is here today thanks to Nintendo. Are you ready for a quick look at like a billion different characters and various pieces of content? This is all brought to you by The Announcer.
Super Smash Bros. Ultimate will be out on December 7 exclusively for the Nintendo Switch.
Sony Offers First Look at Monster Hunter Movie
- Created by: Shawn Zipay
- Published: 11-20-2018, 04:25 PM
- 0 comments
Sony Offers First Look at Monster Hunter MovieToday, Sony offered up the first ever look at what the Monster Hunter movie is looking like. We've seen a few photos alreadsy before this, but apparently this is the "first look." Hey, who am I to doubt things said on Twitter.
Monster Hunter, the movie, is written and directed by Paul W.S. Anderson. He also did the very successful Resident Evil movies that starred Milla Jovovich. Milla Jovovich is also in Monster Hunter. Paul W. S. Anderson and Milla Jovovich are married....
Civilization VI: Gathering Storm Expansion Announced
- Created by: Shawn Zipay
- Published: 11-20-2018, 03:42 PM
- 0 comments
Civilization VI: Gathering Storm Expansion AnnouncedThe second expansion for Civilization VI was officially announced today after about a day of teasing from 2K and Firaxis Games. Civilization VI: Gathering Storm will be released on February 14, 2019 but what exactly does this new expansion add?
Well, for one thing it adds in an "active planet" where the environment may prove to be a bigger foe than any other civilization out there.
Civilization VI: Gathering Storm will introduce an active planet where geology and climatology...
Battlefield 5 is Now Available... Again
- Created by: Shawn Zipay
- Published: 11-20-2018, 03:14 PM
- 0 comments
Battlefield 5 is Now Available... AgainThe release for Battlefield 5 is here!... Again!... For the fourth time!
It's November 20th which means it's time for Battlefield 5 Standard Edition to be released. This comes after the Deluxe Edition was released on November 15, which came after the Origin Access Premier release on November 9th, which came on the same day as the Origin Access and EA Access early release that allowed you to play for 10 hours.
Needlessly staggered release schedule aside, Battlefield...
Afterlife Aims to Blend Live Action and VR Storytelling into a Unique Experience
- Created by: Shawn Zipay
- Published: 11-20-2018, 02:34 PM
- 0 comments
Afterlife Aims to Blend Live Action and VR Storytelling into a Unique ExperienceSignal Space Labs wants to deliver to you a VR experience unlike anything else you've ever experienced before. Get ready to feel emotionally destroyed once their VR experience, Afterlife, blends live-action with a unique VR experience in Q1 2019.
It will be released for the Oculus Rift, Oculus GO, Gear VR, and on Steam.
ABOUT AFTERLIFE After the tragic death of their family member, a family struggles with the loss of their familial anchor and attempts to mend their fraye...
There are no articles in this category.
new posts
Please log in to your account to view your subscribed posts.
widgetinstance 282 (Latest Blogs) skipped due to lack of content & hide_module_if_empty option.
Tag Cloud
2k activision adventure android apple bandai namco battlefield 4 beta bethesda bf4 bioware blizzard bungie call of duty capcom cd projekt red counter-strike counter-strike: global offensive counter-strike global offensive counterstrike counterstrike global offensive cs-nation cs: go cs:go csgo cs go csn csnation delay destiny dice dlc dota 2 downloadable content e3 e3 2014 e3 2015 e3 2016 e3 2017 ea early access ea sports f2p fighting free free-to-play gamescom gearbox global offensive grand theft auto v gta online hidden path hidden path entertainment horror ios launch trailer microsoft mmo mmorpg mobile multi nintendo nintendo switch pc playstation 3 playstation 4 playstation vita ps3 ps4 psa ps vita quickhits racing release date rockstar rockstar games rpg rumor sale sales figures sega sony sports square enix steam switch tgn total gaming network totalgamingnetwork ubisoft valve virtual reality vita vr wbie wii u windows 10 xb1 xbox 360 xbox one


text:
Srinagar, May 30 : Weather warning for Isolated Thunderstorms (TS), lightning, hail and gusty winds has been extended for next four days in Jammu and Kashmir by the India Meteorological Department (IMD) on Saturday.
However, there is no such warning for Union Territory (UT) of Ladakh while the weather will remain mainly dry, barring isolated rain today and tomorrow. Mirpur in Pakistan-occupied-Kashmir (PoK) was hottest with 40 degree maximum and 26 degree minimum temperature, highest in the region during the past 24 hours.
A IMD spokesman said here today that widespread light to moderate rain or thunder could occur in the UT J&K and isolated light rain in UT Ladakh during the next 24 hours. He further forecast for fairly widespread light rain or thunder showers during the subsequent 48 hours. Extending the warning spokesman said that there could be Isolated TS, Lightening, Hail and 30 km to 40 kmph gusty winds in Kashmir and Jammu till June 3. Earlier, similar warning was issued till May 31, he said adding that there is no such warning for Ladakh region. He said Mirpur in PoK witnessed a warm night after the minimum temperature was 26 degree, highest in the entire region of J&K, Ladakh and POK. Mirpur also recorded the highest of 40 degree maximum temperature on Friday.
This was followed by Jammu where the maximum temperature was 37.3 degree and minimum temperature 25.3 degree with traces of rain, he said. Katra, base camp of Mata Vaishnodevi pilgrimage recorded 34.3 degree maximum and 22.2 minimum temperature, he said adding this was followed by Gilgit (POK) 33 degree maximum and 15 degree minimum temperature.
World famous ski resort of Gulmarg witnessed pleasant weather after the maximum temperature was 16.2 degree, lowest in the region and minimum 6.5 degree after 1.2 mm rainfall. However, famed health resort of Pahalgam, where 19.8 mm rainfall was received during the past 24 hours till 0830 hrs, the maximum temperature was 21.8 degree and the minimum temperature was 5.8 degree.
Muzaffarabad, capital of POK, received 4.5 mm rainfall till 0830 hrs and the maximum and minimum temperatures were 26.1 degree and 12.5 degree during the past 24 hours. Srinagar, the summer capital of the UT J&K recorded 24.9 degree maximum and 11.2 degree maximum temperature with sun playing hide and seek during the past 24 hours. (UNI)


text:
I'm working on supporting some Magento 2.1 issues, and I noticed that all of the 2.1 documentation has been removed. I understand not wanting people to use old docs, but removing it completely seems short-sighted. With 2.2's planned deprecation this month, I'm worried they'll do the same thing again.
I would love to see Magento restore old documentation for those of us still working with merchants that can't/won't update. Thoughts?


text:
13005 Keystone Terrace
- North Miami, FL 33181
- $1,399,000
- 4 Bed(s)
- 3 Baths
- 2,652 Sq.Ft.
Welcome Boat Lovers! This is the LOCATION! on Quiet Cul-De -Sac! WATERFRONT HOME WITH NO FIXED BRIDGES AND QUICK ACCESS TO OCEAN AND HAULOVER INLET! FABULOUS and friendly neighborhood with ACTIVE voluntary association hosting numerous events! Dock your boat behind your home or at the nearby, full service marina. MOVE IN, Remodel or Build your dream home . 24 Hour Guard GATED COMMUNITY, CLOSE TO LUXURY SHOPPING, MINUTES TO BAL HARBOR,and beaches!Home features include:PROFESSIONALLY renovated pool area with natural coral keystone, Salt Water Pool,LUSH LANDSCAPING, Newer STAINLESS STEEL appliances, hurricane impact FRONT doors. WOOD AND STONE FLOORS.MAIDS QUARTERS .EASY TO SHOW
The multiple listing information is provided by the MIAMI ASSOCIATION OF REALTORS® from a copyrighted compilation of listings. The compilation of listings and each individual listing are ©2020-present MIAMI ASSOCIATION OF REALTORS®. All Rights Reserved. The information provided is for consumers' personal, noncommercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing. All properties are subject to prior sale or withdrawal. All information provided is deemed reliable but is not guaranteed accurate, and should be independently verified. Listing courtesy of: The Keyes CompanyReal Estate IDX Powered by: TREMGROUP


text:
MicroRNAs (miRNAs) are approximately 22 nucleotides long non-coding RNA molecules that have been identified as important regulators in gene expression post-transcription. Approximately one-third of human genes are regulated and targeted by miRNAs (1,2). Previous studies have reported that miRNAs are associated with a number of diseases, including cancers, neurodegenerative diseases, diabetes, and inflammation. miR-142-3p is one of the miRNAs, and recent literature has reported its functional effects on various cell lines and diseases. For instance, Lei et al. (3) demonstrated that miR-142-3p influenced the proliferation of non-small cell lung cancer cells through repression of TGFβR1. miR-142-3p have been highlighted as a key molecular player in IL-1β-mediated synaptic dysfunction, which indicated its neuroprotective role in multiple sclerosis (4).
It is well known that idiopathic pulmonary fibrosis (IPF) is a chronic and progressive form of interstitial lung disease characterized by an intricate cytokine network and abnormal deposition of mesenchymal cells (5). Inflammation is triggered by infection or tissue injury involving coordinated recruitment of blood components (plasma and leukocytes) at the site of infection or injury (6,7). These initial events are followed by production of various inflammatory mediators, which include prostaglandins, leukotrienes, prostacyclins, lymphokines, interferon-α (IFN-α), IFN-γ, interleukin (IL)-1, IL-8, histamine, 5-hydroxytryptamine, tumor necrosis factor-α (TNF-α), vasoactive amines, eicosanoids and products of proteolytic cascades (8). Currently, treatment modalities available for IPF are ineffective at halting the disease progression. It is a well-established fact that regulation of gene transcription in health and disease involves several non-redundant mechanisms. Alveolar epithelial cell death has been hypothesized as an initiating mechanism underlying bleomycin-induced lung injury and fibrosis.
Cyclooxygenase (Cox)-2 is a cytokine-inducible enzyme that is present in the nuclear membrane and luminal side of the endoplasmic reticulum (9). It has been reported as a pivotal factor in multiple physiological functions and pathological processes, including inflammation and tumorigenesis (10). Previous studies have shown that Cox-2 has a negative correlation with the expression of several miRNAs, such as miR-26b (11), miR-203 (12), and miR-335 (13).
In the present study, bleomycin was used to injure lung epithelial MLE-12 cells. miR-142-3p expression in cells was altered by transfection with miR-142-3p mimic or miR-142-3p inhibitor, and the changes in cell viability, apoptosis, and secretions of pro-inflammatory factors were detected to assess the impact of miR-142-3p expression on bleomycin-induced injury. In addition, correlations between miR-142-3p and Cox-2 as well as PI3K/AKT/mTOR pathways were detected to reveal the underling molecular mechanism in which miR-142-3p protected MLE-12 cells from bleomycin.
Material and Methods
Cell culture and bleomycin treatment
MLE-12 mouse lung epithelial type II cells were purchased from the American Type Culture Collection (USA). Cells were cultured in HITES (hydrocortisone, insulin, transferrin, estrogen) medium (RPMI1640, 2% FBS, 5 mg/mL insulin, 10 mg/mL transferrin, 30 nM sodium selenite, 10 nM hydrocortisone, 10 nM b-estradiol, 10 nM HEPES). MLE-12 cells were treated with bleomycin from 10 to 100 μg/mL for 24 h. NS-398 (20 μM) was used as a Cox-2 inhibitor.
CCK-8 assay
MLE-12 cells were seeded on 96-well plate with 5000 cells/well. Cell viability was assessed by the cell counting kit-8 (CCK-8, Dojindo Molecular Technologies, Japan). Briefly, after stimulation, the CCK-8 solution was added to the culture medium, and the cultures were incubated for 1 h at 37°C in humidified 95% air and 5% CO2. The absorbance was measured at 450 nm using a microplate reader (Bio-Rad, USA).
Apoptosis assay
Cell apoptosis analysis was performed using propidium iodide (PI) and fluorescein isothiocyanate (FITC)-conjugated Annexin V staining. Briefly, cells were washed in phosphate buffer saline (PBS) and fixed in 70% ethanol. Fixed cells were then washed twice in PBS and stained in PI/FITC-Annexin V in the presence of 50 μg/mL RNase A (Sigma-Aldrich, USA), and then incubated for 1 h at room temperature in the dark. Flow cytometry analysis was done by using a FAC scan (Beckman Coulter, USA). The data were analyzed by using FlowJo software (Tree Star, USA).
Cell transfection
Mature miR-142-3p mimic, miR-142-3p inhibitor and their corresponding controls, i.e., mimic control and inhibitor control, were designed and synthesized by GenePharma (China). The cell transfection was performed using lipofectamine 3000 (Invitrogen, USA) according to the manufacturer's instructions.
Quantitative real time PCR
Total RNA was extracted with TRIzol reagent (Sigma-Aldrich) according to the manufacturer's protocol and 2 µg were reverse-transcribed with the Omniscript RT kit (Qiagen, Italy) using random primers (1 mM) at 37°C for 1 h. Real time PCR was performed in triplicate in 20 mL reaction volumes using the Power SYBER Green PCR Master Mix (Applied Biosystems, USA). All primers were purchased from Invitrogen. Real time PCR reactions were carried out in a MJ Mini™ Personal Thermal Cycler apparatus (Bio-Rad Laboratories, USA). Melting curves were obtained by increasing the temperature from 60 to 95°C with a temperature transition rate of 0.5°C/s. The comparative threshold cycle number (CT) method was used to assess the relative quantification of gene expression. The fold change of the target gene was calculated as 2-ΔΔCT. The internal controls were GAPDH for IL-1, TNF-α, Cox2, and U6 for miR-142-3p.
Western blot
The protein used for western blotting was extracted using RIPA lysis buffer (Beyotime Biotechnology, China) supplemented with protease inhibitors (Roche). The proteins were quantified using the BCA™ Protein Assay Kit (Pierce, USA). The western blot system was established using a Bio-Rad Bis-Tris gel system according to the manufacturer's instructions. Primary antibodies were prepared in 5% blocking buffer at a dilution of 1:1000, and were then incubated with the membrane at 4°C overnight, followed by wash and incubation with secondary antibody marked by horseradish peroxidase for 1 h at room temperature. After rinsing, the polyvinylidene difluoride (PVDF; Millipore, USA) membrane carrying blots and antibodies was transferred into the Bio-Rad ChemiDoc™ XRS system, and then 200 μL Immobilon western chemiluminescent HRP substrate (Millipore) was added to cover the membrane surface. The signals were captured and the intensity of the bands was quantified using Image Lab™ Software (Bio-Rad).
Statistical analysis
All experiments were repeated three times. The results of multiple experiments are reported as means±SD. Statistical analyses were performed using SPSS 19.0 (IBM, USA) statistical software. The P values were calculated using a one-way analysis of variance (ANOVA) and P<0.05 was considered to be statistically significant.
Bleomycin induced cell injury and up-regulation of pro-inflammatory factors in MLE-12 cells
Cell viability decreased significantly (less than 50%) when MLE-12 cells were treated with bleomycin at the concentrations of 50 and 100 µg/mL compared to the control group (P<0.05 or P<0.01; Figure 1A). At the same concentration of bleomycin, apoptosis was found to be significantly higher (more than15%) compared to the control group (P<0.01 or P<0.001; Figure 1B).
The expression of pro-inflammatory cytokines, i.e., IL-1 and TNF-α, were assessed. Significance increases in the expression of these two factors were found after MLE-12 cells were treated with bleomycin. Expression of IL-1 increased significantly by 1.5-fold, at both the doses of 50 and 100 µg/mL of bleomycin compared to the control group (P<0.05; Figure 1C). Similarly, expression of TNF-α also increased significantly by 2-fold at both the doses of 50 and 100 µg/mL of bleomycin compared to the control group (P<0.05; Figure 1D).
Effect of transfection on miR-132-4p expression in MLE-12 cells
As shown in Figure 2A and B, after miR-142-3p mimic and miR-142-3p inhibitor transfection, their expressions were more than 3-fold and less than 0.5-fold, respectively, compared to their corresponding controls. These results indicated that miR-142-3p was successfully overexpressed and suppressed in MLE-12 cells after transfection.
Effect of miR-142-3p overexpression on injured MLE-12 cells and pro-inflammatory cytokines secretion
miR-142-3p overexpression significantly alleviated bleomycin-induced cell viability reduction and apoptotic cells increase (both P<0.05; Figure 3A and B). In addition, miR-142-3p overexpression alleviated both IL-1 and TNF-α overproductions induced by bleomycin (Figure 3C and D). Conversely, miR-142-3p suppression affected MLE-12 cells viability, apoptosis, and IL-1 and TNF-α levels in a completely opposite manner.
Effect of miR-142-3p overexpression on Cox-2 expression on MLE-12 cells challenged by bleomycin
Cox-2 expression was found to be up-regulated by 1.5-fold after bleomycin administration at both 50 and 100 µg/mL doses in MLE-12 cells (Figure 4A). miR-142-3p overexpression could decrease Cox-2 expression induced by bleomycin (50 µg/mL) back to the 1-fold/GAPDH level (Figure 4B). However, miR-142-3p suppression could slightly promote the regulation of bleomycin on Cox-2 expression.
Effect of miR-142-3p on Cox-2 and PI3K/AKT/mTOR pathway in MLE-12 cells
MLE-12 cells were pretreated with 50 µg/mL bleomycin alone or together with 20 μM NS-398 (a Cox-2 inhibitor), and then the expression changes in Cox-2, PI3K, AKT and mTOR were detected by western blotting. As shown in Figure 5, 50 µg/mL bleomycin up-regulated Cox-2 by 1.5-fold compared to the expression in the control group (1-fold), while Cox-2 was decreased to 0.2-fold in cells treated with both bleomycin and NS-398. Besides, down-regulations of p-PI3K (0.7-fold), p-AKT (0.5-fold), and p-mTOR (0.5-fold) were observed in bleomycin treated cells, while these three factors were highly expressed in cells treated with bleomycin and NS-398, with 1.7-, 1.5-, and 1.2-fold, respectively.
miR-142-3p overexpressing cells were then treated with 50 µg/mL bleomycin alone or together with NS-398, and the expression changes of Cox-2, PI3K, AKT and mTOR were assessed again. As shown in Figure 6, miR-142-3p overexpression notably reduced bleomycin-induced Cox-2 up-regulation (1.7- to 0.9-fold), as well as PI3K (0.3- to 1.2-fold), AKT (0.4- to 1.1-fold) and mTOR (0.2- to 2-fold) activation.
In the present study, 50 µg/mL of bleomycin was applied into MLE-12 cells to mimic an in vitro IPF model. After bleomycin administration, cell viability was reduced, apoptotic cell number was increased and two pro-inflammatory factors (IL-1 and TNF-α) were over-produced, which indicated MLE-12 cells were injured by bleomycin. Following miR-142-3p mimic transfection, cell injury and pro-inflammatory factors production were alleviated, indicating the protective role of miR-142-3p in bleomycin-induced injury. The expression of Cox-2 was up-regulated by a high dose of bleomycin, while miR-142-3p overexpression partially abolished the regulatory impacts of bleomycin on Cox-2. Besides, NS-398 was applied together with bleomycin, and we found that miR-142-3p negatively regulated Cox-2 via PI3K/AKT/mTOR signaling pathway.
Over the last decade, miR-142-3p has been emerging as a major regulator of many biological processes, including cell apoptosis and inflammatory response. For instance, miR-142-3p inhibited hypoxia/reoxygenation-induced apoptosis of cardiomyocytes (14) and participated in the regulation of aging-related immune responses (15). In the lung, miR-142-3p is involved in malignancy and has been reported to be an early biomarker for aggressive and recurrent lung adenocarcinomas (16). Furthermore, Carraro et al. (17) demonstrated that miR-142-3p balanced proliferation and differentiation of mesenchymal cells during lung development, which indicated a potential role of miR-142-3p in lung diseases such as IPF in which cellular proliferation and differentiation of mesenchymal cells is unbalanced. In the present study, miR-142-3p overexpression exerted protective roles in bleomycin-induced injury in MLE-12 cells, and cell apoptosis and production of pro-inflammatory factors were inhibited after miR-142-3p mimic transfection. These findings further confirmed the hypothesis proposed by Carraro et al. (17), that miR-142-3p might be a potential therapeutic alternative for IPF.
Cox-2 is a critically important mediator of apoptosis and inflammation that significantly influences tumor cells fate (18,19). For example, Cox-2 induction and subsequent Cox-2-dependent activation of PPARγ as a mechanism by which lovastatin lactone induced human lung cancer cell death (20). In the normal lung, Cox-2 has been also reported as a pivotal factor in the activation of the apoptotic and inflammatory pathway (21). In the current study, Cox-2 was up-regulated after bleomycin administration, which indicated that apoptosis and inflammation of MLE-12 cells were induced via regulating Cox-2. Additionally, miR-142-3p overexpression could suppress Cox-2 expression, which provided the first evidence that miR-142-3p protected bleomycin-induced injury via negative regulation of Cox-2.
PI3K/AKT/mTOR is a major pathway mediating cell survival by promoting cell proliferation and inhibiting apoptosis (22,23) and is emerging as a potential therapeutic target for IPF (24). Previous studies have also reported the correlations between Cox-2 expression and PI3K/AKT/mTOR signaling pathway (25). A study in human renal mesangial cells suggested that PI3K/AKT activation participates in TGF-β-mediated induction of Cox-2 protein expression (26). The findings in this study were in line with these previous studies that the up-regulation of Cox-2 induced by bleomycin had a negative correlation with the activation of PI3K, AKT and mTOR. Besides, miR-142-3p overexpression could alleviate bleomycin-induced up-regulation of Cox-2 and the inactivation of PI3K/AKT/mTOR. Moreover, NS-398 was used in this study to confirm the regulatory role of Cox-2 in PI3K/AKT/mTOR signaling pathway. Administration of NS-398 in the lung alleviated bleomycin-induced fibrosis in mice by inducing apoptosis and inflammation (27). Thus, we inferred that miR-142-3p protected bleomycin-induced injury in MLE-12 cells via regulating Cox-2 and PI3K/AKT/mTOR signaling pathway.
In conclusion, this study demonstrated that miR-142-3p overexpression protected bleomycin-induced injury in lung epithelial MLE-12 cells possibly via regulating Cox-2 expression and PI3K/AKT/mTOR signaling pathway. These findings provide evidence that miR-142-3p may be a therapeutic strategy for IPF treatment. Further investigations are still needed to reveal the exact underlying molecular mechanisms in which miR-142-3p functions in lung epithelial cells.


text:
- 2020-2021 Athletic Physical Forms
- 2020 Athletic Physical Information
- 2020-2021 Latta Retirees
- 2020-2021 Online Enrollment Video & Information
- 2020-2021 School Calendar (Revised)
- 2020-2021 School Supply List - PreK - 5th Grade
- 2020-2021 Teacher of the Year
- 2020-2021 Wen-GAGE Account Requests for Online Enrollment
- 7-1-2020 Letter from Superintendent Johnson
- 7-2-2020 Corona Virus Letter
- 7-2-2020 Latta School 2020-2021 Feedback Survey
- Chickasaw Honor Club Program Update
- COVID-19 Updates
- Distance Learning Explanation Video
- EBT for Children--Apply NOW
- Educational Distance Learning Resources
- Family Guides Help Parents Teach at Home
- Oklahoma High School Sports Historical Society
- Pontotoc Co. Class of 2020 Video published by PCDFC!
- Pontotoc Technology Center
- Proposed New Elementary School & Ag Building
- Renaissance Place
- School Law Book
- Staff Mail
- State Department
- Text Notification System Setup Instructions
- Updated>COVID-19 Updates from Superintendent Cliff Johnson
- WenGAGE
Jul 09 Online Enrollment is open today! Primary Guardians may enroll their students beginning with the last names A-G. Please watch the videos provided by Wen-GAGE
- View more notifications.
District News
Letter from Superintendent Johnson available in the Quick Links on the right side of webpage. In an effort to mitigate the spread of COVID 19, Latta School will be offering online enrollment this year for all students. To assist us in preparing for online enrollment, we need your help. The guardian/parent that is listed Contact Sequence one in Wen-GAGE will be the ONLY one with access to the Student Records Online Enrollment Portal. If you already have a Wen-GAGE account with your username and password, you will be set to go when the online enrollment portal is opened up. If you do not have your username and password, please email email@example.com to request your information. #lattapanthers #thelattaway . . .
Panther News
Latta Pre-Kindergarten is seeking information that will help with the 2020-2021 enrollment. The parents of students wishing to attend Latta's Pre-Kindergarten and live in the Latta School district need to call the elementary office by April 27th . Please contact us at (580)332-2092 and speak with Mrs. Kessinger or Mrs. Lancaster. Congratulations District Vocal Music Students: Bailey Howe and Elizabeth Crawford --Superior on their vocal solos and advance to OSSAA State. Middle School-- Aspen Fullerton , Emma Skinner and Lucy Shires --Superior rating for their Trio. Congratulations to DECA State Winners: the following students were finalist. Ryler Rich won the DECA . . .
Latta Mission Statement
LATTA PUBLIC SCHOOLS MISSION STATEMENT Latta Public Schools will provide the opportunity for a challenging and relevant educational experience that will empower students to be productive, confident, and successful citizens. Core Values Integrity —a reflection of high moral character within oneself Honor —a dedication to the principles of right and wrong within one's family, school, and community Dependability —being faithful to one's word Commitment —a pledge to do one's best in all circumstances Accountability —accepting responsibility for actions that affect oneself and others . . .


text:
Gi Conduit Pipe, Galvanized Pipes, Conduit manufacturer / supplier in China, offering EMT Standard Hot DIP Gal Metal Conduit, 3ply Earloop Protective Disposable Face Mask, Medical/ Protective/Safety/ Disposable 3ply Face Mask and so on.
Buy high quality Hot Dip Galvanized Razor Wire For Wire Fence by Hebei Anping County Shengda Hardware Wire Mesh Co.Ltd. Supplier from China. Product Id 726164.
China Factory Galvanized Wire, 2.5mm Hot Dipped Galvanized Wire, Gi Wire manufacturer / supplier in China, offering 2.5mm Hot Dipped Galvanized Wire, Disposable Face Mask, Face Mask. 7 Days Get Your Home, Disposable Face Mask, Fiber Face Mask and so on.
Cable Tray, Cable Ladder, Cable Trunking manufacturer / supplier in China, offering Zinc Plated or Hot DIP Galvanized Wire Mesh Cable Tray Prices, Factory Wholesale Disposable 3 Ply Prevent Medical Mask Face, Durable Stainless Steel IBC Container Tank for Liquid and so on.
Wuxi Baile Sheet Metal Corp. Supplier from China
Strut Channel, Strut Channel Accessory, Strut Support System manufacturer / supplier in China, offering Hot DIP Galvanized 1 5/8 Slotted Strut Channel, Factory Wholesale Disposable 3 Ply Prevent Medical Mask Face, Durable Stainless Steel IBC Container Tank for Liquid and so on.
Chain, Chain Block, Chain Hoist manufacturer / supplier in China, offering Non Woven Disposable Face Mask / Anti Dust Face Mask, Manual Lever Type Hsh V Durable Lifting Chain Hoist / Lever Block, Lifting Equipment D Type Alloy Steel Chain Block / Chain Hoist and so on. Hot DIP Galvanized Eye and Eye Type Commerial Wire Rope Turnbuckle
Wuxi Baile Sheet Metal Corp. Supplier from China
About product and suppliers345 galvanized pipe applications products are offered for sale by suppliers on A wide variety of galvanized pipe applications options are available to you, There are 345 suppliers who sells galvanized pipe applications on , mainly located in Asia.
Tianjin Ehong International Trade , Experts in Manufacturing and Exporting Steel Pipe, Steel Bar and 3320 more Products. A Verified CN Gold Supplier on .
Hot Dipped Zinc, GI, Galvanized steel corrugated galvanized zinc roof Galvanized Steel 0.18mm 20mm thick galvanized steel sheet 2mm thick Hot dip galvanized steel sizes galvanized sheet metal Wholesale and Dropshipping CE/FDA/FFP2 Certified IN STOCK 50 PCS Disposable 3 layered Protection Breathable Earloop Face Mask. $15.90 $19.80. 5
SUPEREAL Professional galvanized steel manufacturers and suppliers in China, providing bulk products for sale. Welcome to wholesale the best galvanized steel at competitive price from our factory.
China Galvanized Iron Wire/Electric Galvanized and Hot DIP Galvanized 16g 20g 22g, Find details about China Galvanized Binding Wire, Galvanized Iron Wire from Galvanized Iron Wire/Electric Galvanized and Hot DIP Galvanized 16g 20g 22g Anping Xingmao Metal Wire Mesh
Galvanized Wire, Stitching Wire, Staple Wire manufacturer / supplier in China, offering Hot Dipped Galvanize Wire/Galvanized Iron Wire/Binding Wire, Disposable Earloop Face Mask, Level 3 Respirator Mask, Medical Standard Coronavius Mask and so on.
China Hot Dipped Galvanized Square Boat Nail, Find details about China Boat Nail, Square Nail from Hot Dipped Galvanized Square Boat Nail PINGDU MINGZONG HARDWARE FACTORY
Buy best price chain link fence fittings and find similar products on
With the excellent properties such as high strength, good toughness, easy machinability and good weldability, Baosteel's hot rolled steel products are widely used in ship, automobile, bridge, building, machinery and pressure vessel and other industrial applications Cold forming Mainly used to manufacture the general machining components such as gear, electrical instrumental box, jack casing
Anchor Chain & Accessories. If you are looking to replace your marine anchor chain look no further! Fisheries Supply stocks all the best types of boat anchor chain including High Test, BBB and Proof Coil. You can buy our chain by the foot or by the barrel ensuring
China Galvalume Coils in Roofing Sheet Zinc Corrugated Iron Steel, Find details about China Steel, Steel Coil from Galvalume Coils in Roofing Sheet Zinc Corrugated Iron Steel Zhejiang Lianxin Steel Plate Technology


text:
- Home
- Logo & business card
- Logo & business card contests
- Have existing logo and biz card, need different flavor for weddings
richam84 got their new logo & business card by running a design contest:
Have existing logo and biz card, need different flavor for weddings
Check out richam84's Logo & business card contest…
The Brief
Wedding Service
Design inspiration
The Brief
Design inspiration
About us
Wundereel is a video editing company with an existing brand that serves action sports enthusiasts to produce cheap, fast-turnaround highlight reels of their action sports footage. We are launching a new product targeted specifically to brides and grooms-to-be. We rent GoPro cameras to a soon-to-be-married couple and they distribute the cameras to friends. After sending the cameras back to us, we make a wedding video from the footage. Our prices start at $500 and we also offer additional services such as one or two professional videographers to also shoot onsite.
The Brief
Color inspiration
Other color requirements
I'd like to explore the following colors: Let designers make suggestions:
The Brief
Design style
The Brief
Other notes
BRANDING CONCEPT - We want a logo and brand that retains some aspects of our current branding so it fits in as part of a cohesive portfolio of offerings from Wundereel. For example, our website: https://www.wundereel.com/ weddings landing page: https://www.wundereel.com/l/wedding current business card front: https://www.dropbox.com/s/5vmzq4khtczf9v5/2015-02%2C%20WR%20Business%20Cards%20layout%20v07.pptx?dl=0 explainer video: https://vimeo.com/wundereel/review/141347379/bc6e2249d2 - Conveys a general wedding feel: artistic, romantic, handmade, simple, beautiful, artisan, etc. E.g. weddingwire.com, theknot.com, mywedding.com, weddingtonway.com. - But is differentiated by: Fun, social, our videos show what really happened, your guests having fun, More instagram than high production value videos, not always the wedding video you would want to show your grandmother. LOGO DESIGN - As explained in the branding concept, we would like to retain some of the visual aspects of our current logo, so that it fits as an extension of our existing brand. Some ideas along these lines we've considered are: 1. Retain basic logo silhouette but change to a pastel orange/purple/blue/yellow and/or 2. Add weddings somewhere to the logo to indicate what we do since the Wundereel name was not specifically selected to signal anything about weddings. E.g. "Wundereel Weddings" or "Weddings by Wundereel" and/or 3. Add an artistic/abstract silhouette of a wedding-themed item such as a bouquet, ring, couple, etc. and/or 4. Use existing logo silhouette but change Wundereel to something more feminine and artistic. and/or 5. anything else you can come up with!
US$700 Custom package
Every design category has flexible pricing for all budgets. Logo & business card starts at €409.
File deliverables
Full copyright with production-ready files for digital and/or print.
It all began with a design brief.
A quick, interactive guide helped them understand their design style and captured exactly what they needed in their logo & business card.
Designers across the globe delivered design magic.
design entries
You'll get loads of concepts from experienced designers around the globe.
Work with talented, professional Logo & business card designers to turn your ideas into reality.
Select your favorite custom Logo & business card (Or two! Or three!). And the design is all yours.
richam84 collaborated with designers to refine their ideas
Rate designs
When design entries come in, you can rate them so designers know what you're looking for in your logo design.
Give feedback
99designs has great collaboration tools so you can pinpoint and capture your ideas
And then… they selected a winner!
Client review
Life Clothing
Amazing work! Took the time to research the project and invested a lot of time to bring our vision to fruition. Will not hesitate to work together on future projects!
Along the way, they met lots of talented designers…
We think contests are a super fun way to get design.
Recently completed contests:
Mini Matrimonies Logo Needed For Micro Wedding Ceremonies!
Launching a new concept of micro weddings. A micro wedding is an intimate affair, typically with no more than 50 guest
Completed project
We need a powerful new logo to relaunch our company after COVID 19
Weddings, Corporate Events, Galas, Music & Community Festivals
Bachelorette-Themed Website Mockup
This website will be featuring the best bar crawls in each of the main cities in all of the states in the U.S. Will be g
Logo für individuelle Goldschmiede für Trauringe
handgefertigte, sehr hochwertige Trauringe aus Edelmetall Einzelgeschäft, keine Kaufhauskette Zielgruppe: solvente Paa
Create sophisticated logo for wedding service based in London
online shop with event service for hen/ bachelor parties
Diamond Bus Co
High end transportation for weddings, corporate & private events
Catchy Trendy logo for Wedding and Elopement Colorado company
We offer pop up weddings that we design and people can enjoy. Its like an upgraded version of ELOPING. The tagline is "a
Modern Farm wedding venue with horses
this is going to be for a venure with horses for weddings/ birthdays etc. its it is for a farm, country setting and with
Need event planning/design company logo: minimalistic typography - luxe but simple
Wedding planning & design company- marketing to authentic couples
Awarding winning Lancashire boutique looking for new logo
Boutique specialising in bridal gowns & prom dresses. Home to top designers, exclusive pieces, accessories & friendly, w
MBR Logo & business card
Wedding Planners and Corporate Events


text:
Food truck catering for your next event. Fast, easy and delicious. Book it here.Get Started
"Life's too Short to Eat Bad BBQ", it says across the top of the menus at 407 BBQ, and we can't think of a truer sentiment. Starting with just a single BBQ trailer just outside Dallas, 407 quickly began to expand, launching 3 more trailers, a full-service catering option, and finally landing themselves in a shop of their own. Even through the success of their brick-and-mortar, 407 is still smoking and rubbing their meats to perfection with said catering service, serving the hungry folks of Argyle and beyond.
We're talking real-deal Texas BBQ here, so you can expect brisket, hot links, smoked turkey, and a whole lot more. In the sides department, 407 is whipping up the classics like baked beans and mac & cheese, but they're also cooking some specialties like green chile cream corn and pineapple slaw. Though desserts can be an often overlooked facet of BBQ, 407 isn't skipping on that either; homemade peach cobbler, banana pudding, and more are in the picture. This is a trailer you need at your next event. Book 407 BBQ today.
Texas Twinkies — Brisket-stuffed jalapeños wrapped in bacon. Yes you read that correctly.
No upcoming events currently listed.