text:
About video
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About Faraday
As a technology partner to Poplar, Faraday helps you take predictive marketing to the next level, enhancing your ability to find, convert, engage, and retain more customers. Leverage Faraday's national consumer database with 270 million US customer profiles, end-to-end machine learning pipeline, and PhD-level data scientists to drive growth with actionable AI.
Faraday & Poplar
With the Faraday Poplar integration, you can push the customer data from your Faraday models directly to Poplar on a scheduled basis and leverage direct mail in your marketing mix. Combine AI-optimized predictions with the unique features of Poplar to deliver personalized & engaging, multi-channel messaging to your top customers and prospects.
Faraday predicts consumer behavior for brands seeking an edge in data, letting you work smarter by bringing data science out of the lab and into your hands.Learn MoreLearn More
Drip is a sales & marketing automation platform designed for ecommerce.Learn MoreLearn More
Email marketing, ads, landing pages, and CRM tools to grow your business on your terms. Get the word out with email, social ads, and make your life easier.Learn MoreLearn More
Integrated email marketing, marketing automation, and small business CRM.Learn MoreLearn More

subset: commoncrawl


text:
Cawbird is a modern and lightweight Twitter client for the GNOME 3 desktop. It
features inline image and video preview, creation of lists and favorites,
filtering of tweets and full text search. Cawbird is able to manage multiple
Twitter accounts.
Cawbird is a fork of Corebird, which became unsupported after Twitter disabled
the streaming API. Cawbird works with the new APIs and includes a few fixes
and modifications that have historically been patched in to IBBoard's custom
Corebird build on his personal Open Build Service account[1].
Due to changes in the Twitter API[2], Cawbird has the following limitations:
All limitations are limitations imposed by Twitter and are not the fault of the
Cawbird client.
[1]: https://build.opensuse.org/project/show/home:IBBoard:desktop
[2]: https://developer.twitter.com/en/docs/accounts-and-users/subscribe-account-activity/migration/introduction
Snaps are applications packaged with all their dependencies to run on all popular Linux distributions from a single build. They update automatically and roll back gracefully.
Snaps are discoverable and installable from the Snap Store, an app store with an audience of millions.
If you're running Kubuntu 16.04 LTS (Xenial Xerus) or later, including Kubuntu 18.04 LTS (Bionic Beaver) and Kubuntu 18.10 (Cosmic Cuttlefish), you don't need to do anything. Snap is already installed and ready to go.
Versions of Kubuntu between 14.04 LTS (Trusty Tahr) and 15.10 (Wily Werewolf) don't include snap by default, but snap can be installed from the command line as follows:
sudo apt update
sudo apt install snapd
To install Cawbird Twitter Client, simply use the following command:
Publisher: gskinner
Flokk Contacts
Publisher: Flutter Team
Flutter SDK
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Code editing. Redefined.
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The IDE for Android
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Publisher: CodeUX.design e.U.
Open Source Password Manager with Keepass file support.
Publisher: Thorsten Lorenz
2D Multiplayer Space Battle Game
Publisher: Torsten Paul
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Publisher: Alan Pope
The always-incognito web browser
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Publisher: Canonical
Lightweight Kubernetes for workstations and appliances
Publisher: librepcb
EDA software to develop printed circuit boards
Browse and find snaps from the convenience of your desktop using the snap store snap.
Interested to find out more about snaps? Want to publish your own application? Visit snapcraft.io now.


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Embargoed 7.00a.m. 3 October 2016
Mountview Estates P.L.C.
Miscellaneous: Announcement regarding the Sinclair Family Concert Party
3 October 2016
Announcement regarding the Sinclair Family Concert Party
("Mountview" or "the Company")
The Company has previously notified its shareholders of the existence of a concert party consisting of certain members of the Sinclair family, related Sinclair family trusts and companies under the control of certain Sinclair family members (the "Concert Party") as more particularly set out in the Appendix to this announcement.
In September 2013, the Concert Party entered into a revised agreement (the "Concert Party Agreement") setting out certain terms of conduct between members of the Concert Party including the event of an offer being made for the Company. The concert party members are not bound under the terms of the Concert Party Agreement to vote together at a general meeting of the Company. The Concert Party Agreement came to an end, in accordance with its terms, at 5.00pm on Friday, 30 September 2016.
The Concert Party has agreed in principle to extend the Concert Party Agreement, in the same terms, until 30 September 2019; this amendment to the Concert Party Agreement is currently being finalised and a further announcement will be made once this has been completed.
This concert party continues to have control of voting rights over 2,087,681 ordinary shares representing approximately 53.54 per cent. of the Company's issued share capital.
APPENDIX - Sinclair Family Concert Party Members
Anray Limited
Mrs Phyllis Esme Cullen
Mrs Elizabeth Langrish-Smith
Miss Emma Lucy Langrish-Smith
Mr James Langrish-Smith
Mr Matthew Langrish-Smith
Scorpion Estates Limited
Mrs Susan Mary Simkins
Mr Ronnie Simkins
Miss Louise Simkins
Mr Thomas Simkins
8532630 Canada Inc.
Mrs Daphne Sinclair
Mr Duncan Morrall Sinclair
8532729 Canada Inc.
Sinclair Estates Limited
Viewthorpe (Old) Limited
Viewthorpe (Subco 2) Limited
Mrs Angela Williams
Mr Rupert Williams
Frank & Daphne Sinclair Grandchildren Settlement
~ Ends ~
For further information on the Company, visit: www.mountviewplc.co.uk
SPARK Advisory Partners Limited (Financial Adviser)
Miriam Greenwood
0203 368 3553
Mark Brady
0203 368 3551
13:00 | Monday, January 20, 2014
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text:
The Ashley and Sierra Memorial Foundation
To honor and celebrate the lives of two best friends who lost their life together in a car crash in 2004, the families of Ashley Lynn Morgan and Sierra Lynn Cerrone created this fund which has been supported by many friends and family members. This fund provides scholarships to graduating students who reside within the territorial limits of the Monticello Central School District and exhibit well-rounded scholarship and vocational aptitude geared to the service of others and the ever-changing needs of society. Click here to apply today!
Click here to download a Driving Record Request Form.
Click the links below for various news articles:
Charges Likely in Crash that Killed Three Teenagers
Teen Faces Trial Over Death of 3 in SUV Crash
Teenage Driver Convicted of homicide
Teen sentenced for deadly crash
Crash scene echoes triple-fatal
There are no upcoming events.
- CFOS Awards More than $400,000 in Scholarships
- CFOS Closes Successful Fiscal Year of Giving and Announces Michael Bonura as New Chairman of Board of Directors
- Orange and Sullivan COVID-19 Response Fund Grants are Available Through CFOS
- Orange and Sullivan COVID-19 Response Fund Addressing Escalating Needs
- Hudson Valley Gives Raises Half a Million Dollars for 145 Local Nonprofits with Virtual Fundraising Day


text:
Grass Fed, Grain finished. No hormones, growth simulators, or antibiotics and minimal vaccines.
A cut from the beef chuck primal, arm roast is also called round bone pot roast, arm chuck roast and arm pot roast. Though braising is often the preferred method for cooking an arm roast, marinating for 2-5 days followed by slow baking will yield a tender flavorful roast.


text:
3 edition of Directory of international and regional organizations conducting standards-related activities found in the catalog.
Directory of international and regional organizations conducting standards-related activities
1983 by U.S. Dept. of Commerce, National Bureau of Standards, For sale by the Supt. of Docs., U.S. G.P.O. in Washington, D.C .
Written in English
Edition Notes
|Maureen A. Breitenberg, editor.
|NBS special publication ;, 649
|Breitenberg, Maureen A., United States. National Bureau of Standards.
|LC Classifications
|QC100 .U57 no. 649, TA368 .U57 no. 649
|The Physical Object
|vii, 359 p. ;
|Number of Pages
|ID Numbers
|Open Library
|LC Control Number
Portuguese cooking
Homeric seafaring
Police warnings
ethics of school administration
Trace-element geochemistry of postorogenic granites from the northeastern Arabian Shield, Kingdom of Saudi Arabia
Young American medals regulations.
Economics of farm size in the Washington-Idaho wheat-pea area
Holt Science & Technology
sermon on II Corinth. V. 6, occasioned by the death of John Ogilvie ...
Türkce-ingilizce sozluk.
COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this 's WebJunction has pulled together information and resources to assist library staff as they consider how to handle.
Get this from a library. Directory of international and regional organizations conducting standards-related activities. [Maureen A Breitenberg. Get this from a library. Directory of international and regional organizations conducting standards-related activities.
[Maureen A Breitenberg; National Institute of Standards and Technology (U.S.);]. texts All Books All Texts latest This Just In Smithsonian Libraries FEDLINK (US) Genealogy Lincoln Collection. Full text of "Directory of international and regional organizations conducting standards-related activities".
Many other organizations, of course, review standards when their interests are affected, but they are not in the mainstream of standards development. The standards of the 20 major private sector standards developers (Figure 2) constitute more than 75 percent of the nongovernment standards database.
USNC Toolbox of Reference Documents. USNC/IEC Training. Governance Committees. US-held ISO Secretariats. Accreditation of U.S. TAGs to ISO Committees.
U.S. TAGs to ISO. U.S. TAGs to ISO/TC U.S. TAGs to ISO/TC U.S. TAG to ISO/PC U.S. TAG to ISO/TC Procedures, Guides and Forms. Regional Programs. Europe, Middle East and Africa. as a regional organization, has been consistently enga-ged in trying to resolve the prolonged conflict of Soma-lia. IGAD member states have committed their resources, time and energy in dealing with this conflict, essentially neglected by the international community.
The major obstacles to various peace initiatives, however, are within Size: 2MB. peacekeeping and enforcement action capacities. Many of the regional and subregional organizations faced the same resource constraints in the conduct of their peacekeeping activities, thus highlighting the importance of matching resources to mandates, irrespective of which organization has been assigned to implement those Size: KB.
New ISO standard will take its breath away. One of the most effective ways of preventing fires in buildings is to reduce the level of oxygen in the air. The world's first International Standard for oxygen reduction systems has just been published. the organization that sets recommended practices and standards for care and processing of devices and systems is.
IAHCSMM CRCST 8TH EDITION - CHAPTER 5 (Complete) 86 Terms. onemoret1me. Central Services Chapter 5 20 Terms. sar Northstar - Equipment Sterilization and Maintenance 40 Terms.
American National Standards Institute is a private, nonprofit organization that identifies public requirements for national safety, engineering, and industrial standards and coordinates voluntary standardization activities of concerned organizations.
Page International Organizations book. Read 2 reviews from the world's largest community for readers. Start by marking "International Organizations: Principles and Issues" as Want to Read: examines the activities of salient regional organizations and non-governmental organizations, as well as multinational corporations/5(2).
Membership overlap with Shanghai Cooperation Organisation, South Asian Association for Regional Cooperation, Organization of American States, African Union, Economic Cooperation Organization, Union of South American Nations, Arab League, Mercosur or Mercosul Organization of the Black Sea Economic Cooperation:%.
A standards organization, standards body, standards developing organization, or standards setting organization is an organization whose primary activities are developing, coordinating, promulgating, revising, amending, reissuing, interpreting, or otherwise producing technical standards that are intended to address the needs of a group of affected adopters.
Most standards. Project Proposal Writing THE REGIONAL ENVIRONMENTAL CENTER for Central and Eastern Europe Written by BESIM NEBIU A project proposalis a detailed description of a series of activities aimed at solving a certain problem. The proposal should contain a detailed explanation of the: • Establishing systems and standards related to developing File Size: 1MB.
The program has been successful in enabling consumer representatives employed by or affiliated with prominent consumer organizations to participate on the ANSI Board and in the Institute's governance activities, as well as in the policy related activities of the International Organization for Standardization's Committee on Consumer Policy (ISO COPOLCO).
Review. 'Organizational Change and Global Standardization is a noble book that proposes unique insights to the study of change management. It initiates distinctive methodologies that study the dynamic system of constant change in-between-ness of transforming spiral and interacting fractals, offers a comprehensive set of examples across multiple Format: Hardcover.
ISO, the International Organization for Standardization, is a nonprofit organization that develops and publishes standards of virtually every possible sort, ranging from standards for information technology to fluid dynamics and nuclear artered in Geneva, Switzerland, ISO is composed of members, each one the sole representative for their.
Research KnowledgeBase Zoning Reform and Code Writing This collection catalogs resources that provide general background, policy and process guidance, and examples of code evaluation reports and noteworthy recent zoning and development codes from various types of communities across the country.
there are in the world intergovernmental organizations, other international bodies, and intergovernmental bodies of special types. Each of these organizations has their own structure and objectives. Beyond their diversity, however, there are common principles that govern the activities of international organizations.
Lists all definitions/terms in the category Standards Organizations of Webopedia. ISO has developed a series of teaching materials that are available for all ISO members to use. These materials were developed as part of ISO's contribution to the University of Geneva Masters' programme Standardization, Social Regulation and Sustainable masters is an interdisciplinary programme including sociology, management and economics.
Recreational activities include field trips, shopping, swimming, and social events as well as a variety of services for seniors.
Offers professional consultation services to agencies that work with people who are deaf-blind. Performs community outreach programs through regional and national offices. Lighthouse Guild International.
AASL (American Association of School Librarians) Affiliate Assembly - Directory of State and Regional Affiliate Organizations List of links to the web sites of state and regional school library media associations which are affiliates of the American Association of School Librarians, a division of the American Library Association.
This is a list of technical standardization organizations. BIPM, CGPM, and CIPM – Bureau International des Poids et Mesures and the related organizations established under the Metre Convention of This list is not limited to ISO members.
^ "Food and Agriculture Organization of the United Nations – Statistics – Standards". - consists of rules that regulate conduct of individuals, businesses, and other organizations in society. - Intended to protect people and their property from unwanted interference from others.
- Forbids individuals from engaging in certain undesirable activities. Standards are everywhere. Can you spot them. This sharable slide show highlights many of the thousands of domestic and international standards developed by ANSI's members, accredited standards developers, and partner organizations.
Though hidden from sight in everyday life, standards are truly everywhere, working behind the scenes to assure that we can live, learn. CHICAGO — Designed to empower leaders to transform teaching and learning, the new "National School Library Standards for Learners, School Librarians, and School Libraries" from the American Association of School Librarians (AASL), published by ALA Editions, reflect an evolution of AASL Standards.
The standards and guidelines found in three previously separate. Washington, D.C. American Planning Association 15th St., NW Suite West Washington, DC Phone: This Directory covers almost institutes in some 90 countries, including academic institutions, governmental institutes, NGOs, private institutes, and international organizations.
The information that is provided on the institutes includes contact details, web page links, names of directors and staff, activities and projects, sources of. Chicago. American Planning Association N. Michigan Ave., Suite Chicago, IL Phone: Fax: Defines the standards of conduct that the society and the community require for the protection of th community as a whole.
Legal Wrongs. Civil or criminal. Civil wrong is a private wrong, such as a tort. (Person or property) Criminal wrong is one in. APA's State Government Affairs program provides insights on state policy trends that have an impact on planning across the country, engages state-level partners, and supports chapter level policy and advocacy programs.
Communications and Engagement (3) + Community or Neighborhood Development (3) + Comprehensive or Long-Range Planning (2) + Economic Development and Revitalization (3) + Environmental and Natural Resources Planning (2) + Facilities Planning Management (2) + Housing Policy, Design, or Implementation (2) + Land Use and Development Regulation and.
The American Federation of Certified Psychics and Mediums Incorporated is an official not-for-profit corporation organized and incorporated under the laws of the State of New York, in March It is the first and only organization of its kind to be state registered and its Articles of Incorporation state approved, in the USA.
The International Labour Organization (ILO) deals with labour issues. Its headquarters are in Geneva, d init was formed through the negotiations of the Treaty of Versailles and was initially an agency of the League of Nations.
It became a member of the UN system after the demise of the League and the formation of the UN at the end of World War II. ALA Student Chapters. The first ALA Student Chapter was created in at the University of Michigan at Ann Arbor.
The most recent ALA Student Chapters were created in Now numbering over 60 (see ALA Student Chapters Directory), Student Chapters have their own character and purpose. ALA Student Chapters enhance students' ALA membership. Looking for abbreviations of RSO.
It is Regional Standards Organization. Regional Standards Organization listed as RSO. Regional Standards Organization - How is Regional Standards Organization abbreviated. Texaco is active in national, international, industry and regional standards organizations.
The International Resource Center is a resource for colleges and universities engaged in international activities, and provides a framework for the key areas that institutions need to be aware of when conducting activities abroad.
Examples include: collaborating with a foreign institution, establishing an office in another country, how to pay independent. The organization that publishes standards and guidelines for maternity nursing is.
Chapman Maternal-Newborn Nursing Chapter 2 42 Terms. garihewitt. Fundamentals of Nursing Values, Chapman Maternal-Newborn Nursing Ch 1 39 Terms. Nurse Chapman Maternal-Newborn Nursing Ch 5 51 Terms.
Through cooperative initiatives with other funders, the National Endowment for the Arts brings the benefit of international exchange to arts organizations, artists, and audiences nationwide. NEA's international activities increase recognition of the excellence of U.S.
arts around the world and broaden the scope of experience of American artists. Chicago. American Planning Association N. Michigan Ave., Suite Chicago, IL Phone: Fax: When it comes to accessing the standards, there's a few things you should know.
First off, the standards are a spiraled curriculum. What this means is they're designed to move with the students as they go from kindergarten all the way through 12th grade. Often when you're finding a particular standard, you need to not just look at the year of the student .


text:
Church icons set, cartoon style
Church with front view in landscape
Montenegro culture symbol set.
Graphic of traditional chinese building for decoration
Tourist take photo of famous pagoda
Catholic priest and nun
Place of worship icons set, cartoon style
Figured coving of a medieval church
Japan landmark with traditional japanese asian character geisha and flowering cherry sakura
The monks are chanting
Illustration of a buddhist temple
Thai style buddhist temple concept
Sanctuary of queralt at pyrenees. catalonia
Bagrati cathedral in kutaisi, georgia
Great church of jvari in mtskheta, georgia
Old armenian christian church made of stone in an armenian village
Vertical shot of a cellar in fountains abbey, yorkshire, england
Orthodoxy monastery at bogolyubovo in summer
Day picturesque view of houses on rock in cuenca
Convento de san miguel. huesca. aragon
"small church among trees"
Chinese garden n zurich
The liberty bell
Medieval castle, church or cathedral spacious hall with wooden stairs on balcony
Wat suthat thepwararam thai templ bangkok thailand
Thai gold mountain temple
Medieval frescoes in the rila monastery.
Frescoes from rila monastery, bulgari
Beautiful shot of an old abandoned house in the middle of a desert near a leafless tree
Aerial shot of an armenian church on a hill with mountain ararat and clear blue sky
Secure wooden doors #4
Beautiful view of the luzhetsky monastery of st. ferapont captured in mozhaisk, russia
Christian priest cleric black cassock with white collar
Dormition cathedral at vladimir in winter
Panoramic view of mtskheta. georgia.
Grand palace outdoors view in bangkok, thailand.
Curchi monastery in orhei, moldova
Aerial shot of a historical building with a dome in a snowy area
Thai north temple
Aerial view of amazing porch with garden umbrella and loungers
Thai style gold decoration concept
Elderly asian buddhist monk smiling at camera in buddhist monastery at river
Monastery sanctuary of queralt
Suzdal in winter. russia
San salvadar monastery in breda


text:
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WASHER MAYTAG MHW5630HW0 Installed parts ran diagnostics through ok. No leaks all functions ok
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REFRIGERATOR WHIRLPOOL KRMF706EBS01 "Customer says door alarm randomly goes off, Both Switches in fridge compartment are not responding correctly, flickering and they get stuck. Need to replace switches; ordrng 2 W11289496 (switch)s"
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DISHWASHER WHIRLPOOL WDT970SAHB0 Replace adjusters
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WASHER MAYTAG MVWB865GW0 Ran full diagnostic cycle and in speaking the the customer instructed on loading and sorting of the clothing. Also instructed on detergent use and the clean cycle on the washing machine. Not on a solid concrete floor either. Floor is flexing while the unit runs in the spin cycle. Only error code in the unit was off balanced loads.
Lukas F.
WASHER WHIRLPOOL WFW5620HW0 Parts ordered weren't correct. I now have correct part numbers. Tk to reach out to customer about return date.; ordrng 1 W11316252 (harns-wire) and 1 W11319991 (cntrl-elec) and 1 W11322903 (cntrl-elec)
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DISHWASHER MAYTAG MDB8989SHZ0 Replace cover; used 1 W10888684 (COVER) from stock
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REFRIGERATOR WHIRLPOOL JB36NXFXLE01 "Deli drawer pan broken, need to be replaced ; ordrng 1 W10529502 (pan-crispr)"
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OVEN KITCHENAID KFED500ESS03 "Enamel on bottom of top oven is chipping near from edge, took pics, this is not repairable part, no evidence of foil on bottom of oven, customer to follow up with whirlpool and or place of purchase for resolution"
Andrew S.
COOKTOP WHIRLPOOL KGCU467VSS "Valve sent out incorrect again, ordering full valve assy can take off that; ordrng 1 WPW10402611 (manifold)"
Andrew S.
WASHER MAYTAG MVWB835DW4 "F3e1 error, ran calibration and diagnostics through ok, pump stopped, all functions pass, inst customer on clean washer cycle if issue persists send out W11170706"
Lukas F.
ADMIRAL ADMIRAL 0 Picked up heat gun.


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Indy life improvement
A lot of folks join AdultFriendFinder as a way to expand their horizons and step outside their comfort zones to discover more about themselves. A lot of those people are focussing on improving a number of aspects of their social and private lives.
This group can be a great medium to discuss avenues available in the Indianapolis area to improve your life. From adding some culture, losing some weight, joining a sex club, or anything else.
Want to know a good way to start losing weight or a good gym in the area? Just ask. Interested in going back to school, but don't where to get started? Bring it up with the group! Want to get into the lifestyle, but need some advice? Ask the group!
A few rules: A.) we must always maintain respect, understanding, and peoples' privacy B.) no unsolicitated advetisements of any sort. If you're coming through town and looking for somebody to bang you, find that "special" person someplace else. C.) that said, nothing is off limits.... There are no stupid questions. D.) Most important; enjoy yourself!
Topic(s): Advice, Education/Schools/Alumni, Entertainment, Health Issues, Money, Pop Culture, Private Parties, Recreation & Sports, Religion & Beliefs, Science & Technology, Swing clubs
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To link to this group (Indy life improvement) use [group 183888] in your messages.
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High reward for employees, no cost for leaders – the impact of appreciation at work
We've all heard the saying, "A person who feels appreciated will always do more than what's expected." Yet it's often underrated in the workplace and undervalued by leaders. Across organizations where employee engagement and increasing productivity are hot topics, leaders are overlooking easily one of the most inexpensive strategies that could be saving them thousands of dollars each year. How? […]READ MORE


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8 Nov 1940
- After failing to expand from the Kalamas River bridgehead in western Greece, Italian troops in that region began to pull back. In the Pindus Mountains in northern Greece, the enveloped Italian Julia Division also began to attempt to break out for a retreat. ww2dbase [Balkans Campaign | TH]
- RAF bombed Munich, Germany, narrowly missing Hitler. ww2dbase [Bombing of Hamburg, Dresden, and Other Cities | TH]
- French Legionnaires and colonial troops from Senegal and Cameroun landed north and south of Vichy-French-held Libreville, French Equitorial Africa. Heavy fighting began immediately. ww2dbase [Battle of Gabon | CPC]
- Operation Crack: Aircraft of British carrier HMS Ark Royal attacked the seaplane base at Elmas near Cagliari, Sardinia, Italy. ww2dbase [CPC]
- British tug HMS Muria hit a mine and sank off the Isle of Thanet near Dover, England, United Kingdom, killing the entire crew. ww2dbase [CPC]
- German bombers attacked a convoy in the Thames Estuary in southern England, United Kingdom, sinking or damaging several merchant ships. Two Ju 87 Stuka dive bombers were shot down by destroyer HMS Winchester, which soon hit a mine and was damaged. ww2dbase [CPC]
- German bombers damaged Swedish ship Vingaland 300 miles west of Ireland. ww2dbase [CPC]
- Dutch submarine O-22 disappeared in the North Sea 40 miles southwest of Norway; 42 Dutch and 3 British sailors were missing and were presumed dead. ww2dbase [CPC]
- RAF Station Takali was officially operational on Malta. ww2dbase [RAF Takali | CPC]
8 Nov 1940 Interactive Map
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Contributors: Alan Chanter, C. Peter Chen, Thomas Houlihan, Hugh Martyr, David Stubblebine
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We want to thank you for your past support of the Sitka Sound Science Center. Fulfilling our mission would not be possible without the generosity of our members and donors.
Our records indicate that your membership has expired. We sincerely hope that you will renew your membership and continue to support the science research and education programs we offer.
Your membership benefits include unlimited admission for you and guests (number varies depending on membership level), 10% off in our gift shop, invitations to exclusive members-only events, and if you are a Salmon member or higher, you also receive 25% off summer camp registrations.
If you would like to rejoin the Science Center family, there are two ways you can do that.
2020 is shaping up to be an exciting year for the Science Center, and we want to include you. The Mill Building restoration project should be completed before summer; a new spawn shed for the hatchery is in the works, as well as other capital improvements. We are anticipating another busy summer season and are excited to move our retail shop and Ludvig's Chowder back to the Mill Building. Our education program continues to grow and reach more school children every year. Our field station program is expanding, and there are several multi-year research projects being conducted out of our facility.
We look forward to again having you as a part of the Sitka Sound Science Center family!


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Re: I Was a Male War Bride (1949)
Posted: February 11th, 2011, 6:27 pm
charliechaplinfan wrote:We have a competition going about screwball comedy and I haven't included it. I agree with you, it's a really good movie and who else but Cary Grant kept making so many classic comedies for so many years.
Thanks for compliments - CharlieChaplinFan.


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Structural And Functional Relationships
As noted earlier, these data all define sarcopenia in terms of its compositional, rather than functional (strength) aspect. However, there are data suggesting that the decline in strength with age exceeds the decline in lean mass. Studies of change in muscle strength over time have shown declines, no change, or gains in strength over periods ranging from four to twenty-five years. A reduction in type II muscle fibers has been shown in some studies, but no change was found in others. In addition to the quantitative decline in muscle with age, there is a qualitative decline, with reduced force production by single fibers from elderly men compared to young men.
Additional topics
Medicine EncyclopediaAging Healthy - Part 4Sarcopenia - Prevalence, Structural And Functional Relationships, Etiology, Treatment Of Sarcopenia


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Discover Bago Maze
After just 15 minutes of entering through the Bago Maze gates, and what felt like walking around in circles getting nowhere, a thought ran through my mind, "Are we ever getting out of here?".
Just a short 35-minute drive from the CBD and heart of Port Macquarie is one of the World's Longest Hedge Mazes. Bago Maze has 2000m of pathways, hedges and bridges. The Maze is a magical place to wander, run and get lost in. So, one weekend with my partner and stepdaughter in tow, I purchased two adult tickets and a child ticket to enter the Bago Maze.
Upon arrival, we were greeted with beautiful green rolling hills and acres of vines where they produce a range of wine on-site.
After checking in, we were given a mission to not only try to get out of the maze but to also find all the dragons and unicorns that were running loose within the maze, destroying all the hedges. We started walking through the gate, and only a few meters in we were met with our 1st choice: the path had split into two. We looked at each other saying "which way?", and with a simple shrug we chose one of the paths to venture down, winding us further and further into the gorgeous maze and hedges.
Finding a staircase leading up to a tower, we climbed up to be met with a stunning view across the maze and vineyard. We quickly identified where we need to be before climbing back down to continue our journey through the maze. After numerous dead ends, walking around in circles, and a couple of fake exit doors, we finally found the exit and walked out into open grounds. We were free from the hedge maze!
Bago Maze is a great place to keep the kids entertained during School Holidays and fantastic weekend activity for the whole family. Being situated at a winery, Bago vineyard offers wine tasting, as well as meat and cheese platters. You can sit back and enjoy a platter and a wine or two at one of their many tables – located on the grass under the trees or on the undercover deck – while the kids wander through the maze.
Bago Maze is open every day except Christmas Day. For more information and online bookings head to Bago Maze's website: www.bago.com.au
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Belinda is a Port Macquarie native who earned her diploma in beauty therapy and has 6 years of experience in this field. She is currently the Marketing Assistant of Port and Kempsey Central, who knows every nook and cranny of the beauty and fashion industry. Stop by to meet her and chat, or get in touch on Facebook – her advice will never lead you in the wrong direction!


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Web review Previous Next
Chill Panda
Linda Milnes Associate professor, Children and young people's nursing, school of healthcare, University of Leeds, on behalf of the RCN's Research in Child Health community
The aim of this mobile app, which is being tested in the NHS, is to help children and adults to manage feelings of stress and worry.
Nursing Children and Young People. 29, 10, 17-17. doi: 10.7748/ncyp.29.10.17.s20
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Archive for August 2008
Missing in action?
Perhaps not so much "missing in action", as "missing because of in-action".
What am I talking about? Well I went to my diet specialist to have a chat and see if we can do something about my total inability to control my eating. You might remember I lost 40+ kilos just before Xmas, I did make a big fuss of it after all 🙂 Well *since* Xmas I've managed to put nearly all of it back on again!
Ok I have a few excuses… huge number of people to feed over Xmas with the associated temptations… then breaking my toe and being inactive for 6 weeks or so… then coming down with the flu twice… not to mention collapsing with back/hip pains several times… but whatever the excuses, the result has been *bloat*.
The specialists recommendation were in three parts.
1. Back to the diet and exercise… obviously
2. See a specialist about a lap band operation… which I'll be doing tomorrow afternoon.
3. Get off the Internet… well get off my backside and away from the computer which amounts to the same thing.
Well, '1' and '2' can be done simply enough… but in order for them to work… I have to implement '3'. While I spend all day sitting by a computer chatting, reading and the like I'm not moving and not moving. If I'm not moving, I'm not burning calories – and especially not if I'm scarfing down whatever I can find in the cupboard while I'm (not) doing it.
The clear answer is to get off my substantial backside and move around more, preferably out of the house and away from the temptation of the kitchen/larder/fridge – during the day especially!!
The general upshot of this is that I'm letting you know in advance that the blog will only be updated intermittently for a while. I'm assuming I'll be here in the evenings a couple of times a week but I'm not promising. If I do, I'll post whatever I can find worth commenting on. If not… well… I won't! 😀
So for now… I'm off.
Breastfeeding… again.
Yet again the topic of breastfeeding appears in the blog. We've brought the subject up a few times before and each time it's been because some bureacratic nitwit has decided it's wrong… offensive… or liable to scare the horses or something.
This time we have a young woman, discreetly expressing milk to feed her young baby by using a breast pump. She was sitting, *behind a curtain*, in a parents room when she was told off by a *cleaner*!! She was followed back to her place of work by a security guard and a police officer and told a formal complaint was being made against her!!
This piece of sublime idiocy didn't happen in some backward 'hillbilly' state in the USA but in sunny Adelaide in southern Australia. You can read the story here and when you do you'll wonder what sort of lunatic would misunderstand in any way what this woman might have been doing!
The complaint was made by a woman who told the security people that "a topless woman was eating lunch in the parents room". Did anyone make serious enquiries of her about what she was doing? Of course not. It was far easier to just harass her.
Did the female cleaner who stood an *watched* her ask her what she was doing? Of course not… far too hard. What she said was that the parents room was for parents and breastfeeding only – and that she wasn't allowed to eat lunch in there. How embarrassing was that?
The clear and obvious fact the breast pump was doing its thing and she was eating her lunch whilst it did it was of course irrelevant. I'm really almost lost for words about this story… I suggest you read it for yourself.
Just tell me… how *stupid* can the management and security services of Westfield, Marion shopping mall really be??
The homecoming… !!!
In many ways the image belows identifies some of what I love about Australia. The picture shows the Prime Minister, Kevin Rudd, sharing the spotlight with the Leader of The Opposition welcoming home our champions from the Olympic Games.
No partisan politics, no trying to score points off each other… just there on our behalf to let our people know we recognise them for their efforts. Bloody magic I call it… tho some of me has the idea our Kev really just wanted a chance to kiss Stephanie Rice!!
Some things are above politics… most of the athletes for starters!
Good onya guys you came out punching way above your weight. There's nothing more anyone could have or would have expected from you than your best, and that what we got. Here's looking forward to the 2012 London Olympics! 😀
One in four.
In a few weeks time it's likely that the Indonesians found guilty of the 2002 bombing at Paddy's Bar in Kuta, Bali will be executed.
The 'favoured' method of execution in Indonesia is shooting. The condemned is taken at midnight to a deserted location where he (or she) is tied to a stake and blindfolded. The execution squad marches out, a priest, or other religico, offers what succour they can, the command is given, and the guns fire.
The assumption in the West at least has been that all but one of the members of the death squad are given live ammunition… so that they can never be *sure* they fired the fatal shot. In Indonesia this is not the case.
Irish born priest Father Charlie Burrows, has given a graphic version of the execution process that is at odds both with our understanding of the process, and the results. The story was published in The Daily Telegraph and makes pretty unpleasant reading.
The execution he attended was of two Nigerian drug couriers and in his account they took a full seven minutes to die moaning and writhing until they eventually bled to death. The death squad sttod by clearly unsure what they should do.
He tells us that the death squad is made up of twelve men, but only three of them carry rifles loaded with live ammunition. If the accuracy of those three is less than perfect then death is not instantaneous, and the soldiers are obviously under great stress themselves, so their accuracy is almost certain to be 'off'.
Do these people deserve death? I'm no supporter of the death penalty even for the Bali Bombers who killed 202 people and wounded a further 209, Muslims, Christians, Indonesians and Foreigners alike… but if a death penalty *must* be invoked to satisfy whatever local laws are in place then surely the least you could ask for is a clean quick death or you reduce yourself to the level of the people you are punishing!?!
I understood the 'rules' of firing squads dictated that in the event of a 'misfire' that simply wounded the condemned the overseeing officer was duty bound to approach the wounded person and administer a coup de grace to end their suffering. In Indonesia it seems this part of the process doesn't exist and the wounded person is simply left.
What happens if they are simply given non-life-threatening wounds I don't know, but I'd assume they would be hauled up against the post once again and given another volley. Regardless, of the end result, it appears that at Indonesian firing squads, there is just a one in four chance of being hit let alone killed outright.
Conditions of life and death in Indonesia are worse than I'd thought.
London look out…!!
Well… it's only 1433 days to the next Olympics and if the cringe factor is anything to go by then they're in with a chance of pulling off something special.
I remember well the way our mouths fell over with total embarrassment when the Sydney 'exhibition' athletes came riding into the stadium at Nagano on bicycles with inflatable kangaroos on their backs. Dire is an understatement but at least it smacked of fun in the sun and a less reverent atmosphere… which came true with the spectacular success Sydney eventually made of their Games.
So what did London promise. Well be had an aging rocker in Jimmy Page out of the ancient Yardbirds and Led Zeppelin groups… along with some wannabee singer belting out a verse from a song that had some… errr … questionable words in other verses. We also had a double decker bus that folded down to show some sort of montage of London tourist traps. And of course they had queues… umbrellas… and an aging footballer in the shape of David Beckham who might have found it hard to look more embarrassed than he was. It was *so* naff it really made my teeth curl.
No doubt by the time it opens in 2012, most of those images will have been quietly sidelined in the same way as our copulating kangaroos never showed their tails again and the Games will be as big a success as all the others. Right now though… all I can do is shudder.
A whale of a time.
The baby humpback whale found stranded and motherless in Sydney Harbour, and named Colin… later Colette, was finally 'euthanased' in Sydney Harbour yesterday.
There've been all sorts of stories about her and the way she was/wasn't aided by government agencies etc and there is now talk of a *summit* of experts to try to establish guidelines for procedures in the event this happens again.
Wonderful sentiments.
I wonder how many of these experts weeping over the sad loss of this baby whale went off home to eat steak, veal cutlets, sushi and the like? The hypocrisy involved in our relationship with animals is utterly amazing sometimes and you have to wonder just why people can't see the irony in our behaviour, We desperately seek to succour and maintain the life of one *wild* animal who is following the natural course of it's life whilst concurrently imprisoning and killing untold millions of other animals helpless to protect themselves or their offspring from our predation?
I wonder when we'll see a summit of 'experts' gathering to establish guidelines for the abolition of meat in our diets?
Yes there's a real paradox in the way we eat meat, yet feel desperately sorry for the way this animal was treated, but surely things are getting out of hand when we spend millions of dollars protecting *one* animal whilst spending far more millions killing others!!
Christina Applegate strikes a chord.
You have to hand it to Christina Applegate, she's really struck a chord with people suffering from breast cancer in that she has been quite open about the treatment she opted for in her battle with the disease.
For someone so high in public visibility to make it clear she'd decided a bi-lateral mastectomy was the best option to ensure her survival to a ripe old age. We hope this will encourage others in the same position to go for the option rather than try to maintain their bodies intact and risk a recurrence of the cancer.
She seems to be quite confident that she has done the best she can to defeat the disease and that it will never reappear. Hopefully she'll be 100% for a long time to come.
Insane in America
The USA is determined to address what it sees as possible criminal or terrorist potential by temporary workers. It's doing this by deporting people, who have committed an offence, back to their country of origin. This seems quite reasonable, except that they are doing this regardless of the time they've spent in the US, or the length of time since the offence.
In the case of 35 years old Welsh born Zak Ashenhurst, 35, his offence was firing a gun into an empty swimming pool and was committed some 20 years ago. Ok a silly offence, possibly dangerous at the time, but done when he was a child! Not only was he a child, but was recovering from Osteomyelitis which he had feared would kill him and was a little 'stressed' at the time to say the least! He fronted the courts at the time, was fined, and pulled himself back into line. He went on to create a good life.
He married an American girl and had two children Zaky, 12, and Nicki, five. And has worked ever since.
Over the years the Green Card residents use to maintain their right to work in the US expired and was renewed without incident… until a few weeks ago when officials unearthed his old conviction and, using laws unused for decades, decided he was a risk to national security and threw him in prison.
He was there for a month until he was removed from the country and deported back to the UK yesterday.
This ridiculous and unfair decision has left Zak in what is now almost a foreign country, and unsure whether his family, all American citizens, will be allowed to join him!
Nobody is underestimating the dangers of terrorist activity but surely the lunacy of deporting a solid citizen for something he did as a youngster should be obvious even to a faceless bureaucrat? The *most* that should have been done was to use Zak as a role model for the thousands of kids that are running wild in US schools! The example of someone who make a mistake and turned his life around should be a positive message used to help otehrs who might be heading down the wrong path! It should not be a cause for further punishment!
The situation is entirely ridiculous and the UK foreign office should be doing all it can to ensure that his family is allowed to join him… if the want to! After all, these are American citizens effectively being told they must choose between living as a single parent family in their homeland or joining their father in a foreign country and facing an uncertain economic future.
The utter unfairness of it just can't be over stated – though I doubt the US administration would be concerned about this one small family unit, no matter how abysmally it's treated them.
Having trawled around looking for more information I turned up this gem. According to The Star News, this sort of treatment is nothing new. Back in 2004 an 83 year old guy, nominally a 'Frenchman' who had committed a long forgotten minor offence, was deported back to Paris despite having lived in the US for 52 years! He was kept in prison for 7 months before being sent back to a country he knew little about and despite him having nowhere in France to go!
Home of the free? Think again!! Right now in the USA Xenophobia rules.
Wow! British cycling comeback!
The UK Cycling team has excelled this year with them winning most of the gold medals at the Beijing Olympics. Got to be fair… this really is an outstanding result. 🙂
Breaking world records along the way just adds icing to the cake.


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To get a cap rate, you must know expenses, and that is not just taxes, insurance, and debt service!
cap rate and COC numbers.
For quick evaluations, you can use the 50% rule which states that 50% of your gross rent will go towards expenses (operating, capital, and vacany). The balance 50% will go to debt service and cash flow.
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So, at $1400 gross, after expenses, you are at $700 monthly to cover debt service and cash flow. With $140k in debt, you are not left with a whole lot of cash flow, so where did you get 8.3% cap rate?Be careful. The sellers cash flow could have been great all these years because they never fixed anything. First off, $100 a month for repairs is fairly low. Over the long haul, you will incur at least 10% of your gross rents in repairs which in this instance would be $140 monthly. You also did not include accountanting costs, legal fees, evictions, capital expenses, advertising, tenant placement fees, management fees (even if you manage yourself, you still have to pay yourself for your time).
So your gross monthly rent is about 1% of the acquisition price which only means that the income can support PITI and nothing much more above that. To get actual cash flow, you need to be at 1.5% or better at those acqusition prices to get real cash flow which makes me wonder how you came up with the Nike Womens Shorts Black
This is a buy and hold, not a flip. Without taking the roof replacement cost into consideration, my initial calculation of cap rate Nike Shorts Running is 8.3% and cash on cash is about 8.9% for this property. Since I new to real state investement, I wonder what would be the ideal return normally for a buy and hold strategy. I located in Minnesota. Houses I looking at are typical rambler built in 1950 65 with 3 to 4 bedrooms and 1.5 bath, price range is from $130K to $160K. Thanks again,
should I withdraw my offer
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I'm new to the forum. I have a question regarding an offer I made yesterday. I offered to a real estate investment property last night. The owner accepted my offer but they didn have the property disclosure available when I sent my offer. I just received the property disclosure today. I notice the age of the roof is 18 years old, and no repair or replacement work was done since 1994. I feel like the roof is reaching the end of life. I was under the assumption that the roof doesn need to replace soon when I offered. So the owner is telling me although the roof is 18 years old but nothing is wrong with it, and they stand firm with the price. From a real estate investment standpoint, should I withdraw my offer due to the potential roof replacement cost? Thanks,Is this a flip, or a buy and hold? If a flip, and the roof has good appearance, and after your roof inspection, needs no repairs, I would not worry about it. If a buy and hold, I would have concern as you will run into some capital expenses sooner rather than later. To account for that, you either need to have awesome cash flow, or get the price lower to account for said future costs (or get a deferred maintenance credit from the seller)Guys, thanks for your prompt replies.
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T-Mobile Puts Its Cheapest Family Plan on Sale for Summer
Article Comments
Jul 22, 2020, 10:04 AM by Rich Brome @rbrome
T-Mobile is temporarily offering lower prices for accounts with four to six lines on its Essentials plan. Normally $30/month/line, families and small businesses with four lines can now sign up for the Essentials plan for just $25/month/line. Adding a fifth or sixth line is just $20/month/line. Unlike T-Mobile's pricier Magenta plans, Essentials plan rates do not include taxes and fees. The Essentials plan does include 5G data, but limits data speeds during network congestion (giving Magenta-plan customers priority), limits mobile hotspot use to 3G speeds, limits data in Canada and Mexico to 2G speed, and does not include data roaming for other countries. Nor does Essentials include perks like Netflix and Gogo, although it does include T-Mobile Tuesdays.
Nov 15, 2018
AT&T plans to cease offering AT&T Smart Limits, a service that allows parents to control their kids' phones, on November 26. Smart Limits will no longer be available after that date.
Sep 5, 2019
T-Mobile has a new set of "Unlimited 55" plans for customers who are at least 55 years of age. The plans come in three tiers just like its plans for everyone else, and include the same benefits at each tier, they simply cost around 40% less.
Aug 6, 2018
T-Mobile today announced T-Mobile Essentials, a low-cost plan that drops many of T-Mobile's extra services and focuses on the basics. T-Mobile Essentials offers unlimited talk, text, and smartphone data for $60 per month for one line.
Jun 14, 2018
Verizon Wireless today overhauled its unlimited postpaid service plans. The company now offers three tiers of unlimited service that each has its own features.
Apr 2, 2019
Verizon's new Just Kids wireless service plan bundles parental controls and a restricted call list with 5GB of data, for $10/month less than the company's cheapest unlimited plan. The company announced the plan today, and it will be available starting this Thursday.
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NEWPORT BEACH (CBSLA) — Hospital officials at Hoag Memorial Hospital are calling for patients who have recovered from coronavirus to donate plasma so the antibodies could be used to help current patients recover.
Plasma donations helped Ebola patients recover and so Hoag Memorial Hospital Dr. Arell Shapiro, who is in charge of transfusions and lab medicine at the hospital, said he jumped at the chance to join a study on using plasma on ICU patients at Hoag.
"We've treated 58 patients with over 100 units of convalescent plasma," Shapiro said. "We don't yet know the efficacy of how well the plasma has affected the patients' outcome, but we think it's having a good effect on the outcome. The problem is we don't have data from the study analyzed yet."
So far, the hospital has received plasma donations from 249 recovered patients.
The process is similar to giving blood and patients have the potential to help two to three patients with just one donation, Shapiro said.
Hoag Hospital said in a tweet that its urgent care center provides coronavirus active virus and antibody testing, but not rapid testing at this time.
Hoag Urgent Care provides #COVID19 active virus and antibody testing. Please note that rapid testing is not currently available. Make a reservation for an in-person or telehealth visit at one of our locations on https://t.co/juMjqt8p3N. pic.twitter.com/TDqSVLpu8K
— Hoag (@hoaghealth) July 6, 2020
Recovered coronavirus patients who want to donate need to be in good health and be completely symptom-free for at least two weeks to be eligible to donate plasma, officials said.
Information on donating plasma at Hoag Memorial Hospital Presbyterian can be found at hoag.org/COVID.
(© Copyright 2020 CBS Broadcasting Inc. All Rights Reserved. City News Service contributed to this report.)


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A biofilm is a community of microorganisms attached to a surface and embedded in a matrix of extracellular polymeric substances. Biofilms confer resistance towards conventional antibiotic treatments; thus, there is an urgent need for newer and more effective antimicrobial agents that can act against these biofilms. Due to this situation, various studies have been done to investigate the anti-biofilm effects of natural products including bioactive compounds extracted from microorganisms such as Actinobacteria. This review provides an insight into the anti-biofilm potential of Actinobacteria against various pathogenic bacteria, which hopefully provides useful information, guidance, and improvements for future antimicrobial studies. Nevertheless, further research on the anti-biofilm mechanisms and compound modifications to produce more potent anti-biofilm effects are required
No disponible
AssuntosActinobacteria/isolamento & purificação , Streptomyces/fisiologia , Biofilmes/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Actinobacteria/genética , Actinobacteria/efeitos dos fármacos
An endophytic actinomycete strain SKH1-2 isolated from Musa (ABB) cv. 'Kluai Sao Kratuep Ho' collected in Suphan Buri province (14° 54′ 22.5″ N/100° 04′ 50″ E), Thailand, was identified as Streptomyces pseudovenezuelae based on phenotypic and chemotaxonomic characteristics, and 16S rRNA sequence analyses. A chemical investigation led to the isolation of two polyketide molecules from the n-butanol crude extract of the strain SKH1-2 culture broth. The compounds were purified using various chromatographic techniques and identified using spectroscopic methods compared with earlier published data. Compound 1, chartreusin, is known as an anti-Gram (+) bacterial compound and was active against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341 and Staphylococcus aureus ATCC 6538p with MIC values of 3.1, 1.6 and 12.5 μg/mL, respectively. Compound 2, lumichrome, did not show activity against all tested microbes. To our knowledge, this is the first report of chartreusin and lumichrome isolated from S. pseudovenezuelae. Taken together, it could be proved that Thai plant species are valuable reservoirs of interesting endophytic actinomycetes producing several interesting biologically active compounds
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AssuntosPolicetídeos/isolamento & purificação , Streptomyces/isolamento & purificação , RNA Ribossômico 16S/isolamento & purificação , Staphylococcus aureus/isolamento & purificação , Streptomyces/classificação , Musa/química , Musa/microbiologia , RNA Ribossômico 16S/química , Análise Espectral
The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 μg/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds
No disponible
AssuntosAntifúngicos/metabolismo , Candida/efeitos dos fármacos , Rizosfera , Microbiologia do Solo , Streptomyces/isolamento & purificação , Candida/crescimento & desenvolvimento , RNA Ribossômico 16S/genética , Sérvia , Streptomyces/classificação , Streptomyces/genética , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética
Antecedentes: El cribado farmacológico y el uso de productos naturales para el tratamiento de las enfermedades humanas tiene un largo historial que comienza en la medicina tradicional y se extiende hasta los fármacos modernos. La mayoría de los fármacos modernos proceden principalmente de productos naturales. Objetivo. El objetivo del presente estudio fue valorar la actividad inhibidora of 5-(2,4-dimetilbencil) pirrolidin-2-uno (DMBPO) extraído de Streptomyces VITSVK5 sp. marino aislado de muestras de sedimento recolectadas en la costa de Marakkanam de la bahía de Bengala, India. Métodos. El compuesto principal se aisló mediante extracción bioactiva guiada y se purificó mediante cromatografía de columna de gel de sílice. La dilucidación estructural del compuesto principal se efectuó utilizando datos espectrales de las técnicas UV, FT-IR, 1H NMR, 13C NMR, DEPT y HR-MS. Resultados. El cribado sistemático de los aislamientos en busca de actividad antimicrobiana dio lugar a la identificación de una cepa potencial, Streptomyces VITSVK5 sp. (GQ848482). Con la extracción bioactiva guiada se obtuvo un compuesto DMBPO y su actividad inhibidora se examinó frente a cepas bacterianas y fúngicas seleccionadas. DMBPO mostró una actividad máxima frente a Escherichia coli con un valor de la concentración inhibitoria mínima (CIM) de 187mg/ml, seguida de Klebsiella pneumoniae (CIM de 220mg/ml y zona de inhibición de 10,3mm), Staphylococcus aureus (CIM>1.000mg/ml y zona de inhibición de 4,4mm) y Bacillus subtilis (CIM de 850mg/ml y zona de inhibición de 2,6mm). Además, se puso de relieve que DMBPO también fue un inhibidor potente de los patógenos fúngicos oportunistas. Se demostró una actividad máxima frente a Aspergillus niger con un valor de CIM de 1mg/ml y una zona de inhibición de 28mm. Conclusión. El resultado del presente estudio indica que DMBPO posee actividad antibiótica frente a patógenos bacterianos y fúngicos seleccionados y exhibió una mejor actividad frente a hongos que bacterias(AU)
Background. Pharmacological screening and usage of natural products for the treatment of human diseases has had a long history from traditional medicine to modern drugs. The majority of modern drugs are reported to be mostly from natural products. Objective. The aim of the present study was to evaluate the inhibitory activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one (DMBPO) extracted from marine Streptomyces VITSVK5 spp. isolated from sediment samples collected at Marakkanam coast of Bay of Bengal, India. Methods. The lead compound was isolated by bioactive guided extraction and purified by silica gel column chromatography. Structural elucidation of the lead compound was carried out by using UV, FT-IR, 1H NMR, 13C NMR, DEPT and HR-MS spectral data. Results. Systematic screening of isolates for antimicrobial activity lead to identification of a potential strain, Streptomyces VITSVK5 spp. (GQ848482). Bioactivity guided extraction yielded a compound DMBPO and its inhibitory activity was tested against selected bacterial and fungal strains. DMBPO showed maximal activity against Escherichia coli with a MIC value of 187mg/ml, followed by Klebsiella pneumoniae (MIC of 220mg/ml and 10.3mm zone of inhibition), Staphylococcus aureus (MIC of >1000mg/ml and 4.4mm zone of inhibition) and Bacillus subtilis (MIC of 850m/ml and 2.6mm zone of inhibition). Furthermore, DMBPO was found to be a potent inhibitor of opportunistic fungal pathogens too. It showed a maximum activity against Aspergillus niger with a MIC value of 1mg/ml and 28mm zone of inhibition. Conclusion. The result of this study indicates that DMBPO possess antibiotic activity to selected bacterial and fungal pathogens and exhibited better activity against fungi than bacteria(AU)
AssuntosHumanos , Masculino , Feminino , Pirrolidinas/isolamento & purificação , Pirrolidinas/metabolismo , Programas de Rastreamento/métodos , Streptomyces/isolamento & purificação , Produtos Biológicos/isolamento & purificação , Produtos Biológicos/uso terapêutico , Medicina Tradicional/métodos , Medicina Tradicional , Produtos Biológicos/análise , Produtos Biológicos/biossíntese , Produtos Biológicos/farmacocinética , Escherichia coli/isolamento & purificação
A newly identified extracellular laccase produced by Streptomyces ipomoea CECT 3341 (SilA) was cloned and overexpressed, and its physicochemical characteristics assessed together with its capability to decolorize and detoxify an azotype dye. Molecular analysis of the deduced sequence revealed that SilA contains a TAT-type signal peptide at the N-terminus and only two cupredoxine domains; this is consistent with reports describing two other Streptomyces laccases but contrasts with most laccases, which contain three cupredoxine domains. The heterologous expression and purification of SilA revealed that the homodimer is the only active form of the enzyme. Its stability at high pH and temperature, together with its resistance to high concentrations of NaCl and to typical laccase inhibitors such as sodium azide confirmed the unique properties of this novel laccase. The range of substrates that SilA is able to oxidize was found to be pH-dependent; at alkaline pH, SilA oxidized a wide range of phenolic compounds, including the syringyl and guayacil moieties derived from lignin. The oxidative potential of this enzyme to use phenolic compounds as natural redox mediators was shown through the coordinated action of SilA and acetosyringone (as mediator), which resulted in the complete detoxification of the azo-type dye Orange II (AU)
No disponible
AssuntosStreptomyces/isolamento & purificação , Corantes/isolamento & purificação , Lacase/análise , Antibacterianos/farmacocinética
Uptake hydrogenase is an enzyme that is beneficial for nitrogen fixation in bacteria. Recent studies have shown that Frankia sp. has two sets of uptake hydrogenase genes, organized in synton 1 and synton 2. In the present study, phylogenetic analysis of the structural subunits of hydrogenase syntons 1 and 2 showed a distinct clustering pattern between the proteins of Frankia strains that were isolated from different host plants and non-Frankia organisms. The structural subunits of hydrogenase synton 1 of Frankia sp. CpI1, Frankia alni ACN14a, and F. alni AvCI1 were grouped together while those of Frankia spp. CcI3, KB5, UGL140104, and UGL011102 formed another group. The structural subunits of hydrogenase synton 2 of F. alni ACN14a and Frankia spp. CcI3 and BCU110501 grouped together, but those of Frankia spp. KB5 and CpI1, F. alni ArI3, and F. alniAvCI1 comprised a separate group. The structural subunits of hydrogenase syntons 1 and 2 of Frankia sp. EAN1pec were more closely related to those of non-Frankia bacteria, i.e., Streptomyces avermitilis and Anaeromyxobacter sp., respectively, than to those of other Frankia strains, suggesting the occurrence of lateral gene transfer between these organisms. In addition, the accessory Hyp proteins of hydrogenase syntons 1 and 2 of F. alni ACN14a and Frankia sp. CcI3 were shown to be phylogenetically more related to each other than to those of Frankia EAN1pec (AU)
No disponible
AssuntosHidrogenase/síntese química , Bactérias/genética , Filogenia , Streptomyces/genética , Myxococcales/genética
A DNA fragment from the lignocellulolytic actinomycete Streptomyces avermitilis CECT 3339 was cloned using a DNA probe from the xylanase gene xysA of Streptomyces halstedii. The nucleotide sequence analysis revealed two potential ORFs, xyl30 and hd30, encoding a deduced multimodular F/10 xylanase with a binding domain and a secreted glycoxyl hydrolase, respectively. In Streptomyces lividans carrying the subcloned DNA fragment, two xylanase activity bands with estimated molecular masses of 42.8 and 35 kDa (named Xyl30 forms «h» and «l», respectively), were detected by zymograms and SDS-PAGE. The two xylanases had identical N-terminal sequences, suggesting that Xyl30 «l» derived from Xyl30 «h» by C-terminal processing in the culture supernatant. No transcripts of hd30 were detected by RT-PCR. Characterization of the partially purified Xyl30 «h» confirmed the presence of a modular endoxylanase containing a xylan-binding domain, which after processing in the culture supernatant loses the aforementioned domain and thus its capacity to bind xylan (Xyl30 «l»). Xyl30 «h» achieved maximal activity at pH 7.5 and 60 degrees C, retaining more than 50% of its activity from pH 3 to 9 and more than 40% after a 1-h incubation at 70 masculineC. Moreover, in the recombinant host strain up to 400 U xylanase/g medium (dry weight) was produced in solid-state fermentation (SSF) using cereal bran as substrate. The high production yields of this enzyme and its biochemical features make it a good candidate for use in industrial applications (AU)
No disponible
AssuntosXilanos/genética , Streptomyces/enzimologia , Fermentação/genética , Análise de Sequência/métodos , Leveduras/enzimologia
The membrane protein complex translocase mediates the translocation of bacterial proteins. In this complex, the SecY, SecE, and SecG proteins constitute an integral membrane domain. Sequence comparison revealed a potential secG-like gene in the gram-positive soil bacterium Streptomyces lividans. Chromosomal deletion of this gene resulted in a sporulation defect and an overall deficiency in secretion. The SecG-depleted strain was able to overproduce and secrete alpha-amylase, but the appearance of the oversynthesized protein outside the cell was delayed compared to the protein produced by the wildtype strain. SecG deficiency was found to result in more pronounced effects in S. lividans than in Bacillus subtilis or Escherichia coli (AU)
No disponible
AssuntosStreptomyces/imunologia , Translocação Bacteriana/imunologia , Proteínas de Transporte da Membrana Mitocondrial/imunologia , Bacillus subtilis/imunologia , Escherichia coli/imunologia
Objetivo. Evaluar el significado clínico del aislamiento de Streptomyces en distintas muestras clínicas. Material y métodos. Se revisaron historias clínicas de los pacientes con aislamiento de Streptomyces en cualquier muestra clínica durante un período de siete años en un hospital terciario. Resultados. Se aisló Streptomyces en 13 pacientes. todos tenían enfermedades subyacentes. Sólo en un paciente Streptomyces fue considerado responsable del cuadro clínico. Se presenta el tercer caso de infección asociada a catéter por este microorganismo. Conclusiones. Streptomyces habitualmente se aísla en pacientes con patología subyacente. Su aislamiento debe interpretarse en el contexto clínico del paciente para considerarlo significativo
OBJECTIVES: To evaluate the clinical significance of Streptomyces isolates in different clinical samples. MATERIAL AND METHODS: Review of the records of all cases of Streptomyces isolated from any clinical sample at a tertiary Hospital, during a seven-year period. RESULTS: Streptomyces was isolated from 13 patients. All of them had underlying diseases. Only in one patient Streptomyces was considered to have a pathogenic role in the clinical picture. We report the third case of catheter-related infection caused by this microorganism. CONCLUSIONS: Streptomyces is usually isolated from patients with underlying diseases. Before considering them significative, Streptomyces isolates must be interpreted in the clinical context
AssuntosMasculino , Adulto , Humanos , Bacteriemia/etiologia , Cateterismo , Contaminação de Equipamentos , Infecções por Bactérias Gram-Positivas/etiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Streptomyces/crescimento & desenvolvimento , Streptomyces/isolamento & purificação
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text: ERIK ANDERSON/AAP July 30, 2020 723 cases is a bad number for Victoria. But we can't freak out over a single day's figure Ian M. Mackay, The University of Queensland You're right to be concerned but we can't draw too many conclusions from just one or two data points. Instead, we need to look at averages over multiple days.


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Mueller Adjustable Lumbar Back Brace
Where to buy
Mueller Adjustable Lumbar Back Brace
Known for its medical solutions to athletes, Mueller has designed a fully adjustable back brace for all. Its comfortable plastic stays is great for supporting weak lower back muscles to encourage better posture and healthy alignment of the spine. Moreover, this unique brace uses a single pull strap which is specially designed for one-hand tightening.