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Generally once a lease is signed the terms cannot be amended unless all parties agree. The determination of what can and cannot be done is decided by the wording of the original agreement. However, a supposedly "oral/verbal" contract is difficult for a landlord to prove if it becomes an issue for the court. If the tenant did not agree to any amendment action being acceptable when the original contract was entered into, the landlord cannot arbitrarily make changes to the agreement.
Theodore H. Hellmuth has written: 'Lease audits' -- subject(s): Auditing, Commercial leases 'Real estate law' -- subject(s): Mortgages, Real estate development, Law and legislation, Vendors and purchasers, Landlord and tenant 'Missouri leasing guide for non-lawyers' -- subject(s): Landlord and tenant, Commercial leases
Matters such a locks are usually covered in a written lease. If it's not covered or if there is no written lease you probably can. BUT, you must give the landlord a key. The landlord has the right to enter in an emergency and upon reasonable notice to the tenant to inspect the premises. Keep in mind that landlord / tenant relationships without a written lease are generally governed by state law, so the conclusion may differ from state to state. When I was a landlord, there was an option to change the keys for a tenant. I had it done at my cost and held a key. Most locksmiths will not change the keys if you do not own the home. In many commercial leases the tenant is EXPECTED to change the locks and the landlord may not want to even have a key. For example, when renting a self-storage unit, the tenant would typically add his or her own lock to the unit. Upon default or emergency, the landlord would simply break the lock to gain entry, then put on a new lock.
Get StartedA real estate lease is a written agreement between a Landlord (lessor) and a Tenant (lessee) establishing the rights and responsibilities of each party. The Landlord is the owner of real estate (also known as "premises") who leases (rents) that property to a Tenant for the Tenant's use. A "residential" lease applies to real estate used as a residence, while a "commercial" lease applies to business property.A written lease should be prepared and signed whenever property is rented, to reduce the likelihood of misunderstandings between the Tenant and the Landlord regarding the rental arrangement.
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Plumbagin (PLB) has been shown to have anticancer activities in animal models but the role of PLB in prostate malignancy treatment is unclear. sirtuin 1 (Sirt1) and inhibition of Sirt1 enhanced autophagy whereas the induction of Sirt1 abolished PLB-induced autophagy in PC-3 and DU145 cells. In addition PLB downregulated pre-B cell colony-enhancing factor/visfatin and the inhibition of pre-B cell colony-enhancing factor/visfatin significantly enhanced basal and PLB-induced apoptosis and autophagy in both cell lines. Moreover reduction of intracellular reactive oxygen species (ROS) level attenuated the apoptosis- and autophagy-inducing effects of PLB on both Computer-3 and DU145 cells. These results suggest that PLB promotes apoptosis and autophagy in prostate cancers cells via Sirt1- and PI3K/Akt/mTOR-mediated pathways with contribution from AMPK- p38 MAPK- visfatin- and ROS-associated pathways. L Juglans regia J. cinerea and J. nigra.13 A variety of pharmacological activities of PLB including anti-inflammatory neuroprotective anticancer hypolipidemic antiatherosclerotic antibacterial and antifungal effects have been reported in in vitro and in vivo models.13 The anticancer effects of PLB are mainly attributed to the induction of intracellular reactive oxygen species (ROS) generation apoptosis autophagy and cell cycle arrest 13 even though underlying mechanisms are not fully understood. In vitro and in vivo studies by TAK-901 our laboratory and other organizations have shown that PLB induced malignancy cell apoptosis and autophagy via modulation of cellular redox status inhibition of NF-κB activation upregulation of p53 via c-Jun N-terminal kinase (JNK) phosphorylation and inhibition of the phosphatidylinositide 3-kinase (PI3K)/protein kinase B (Akt)/mTOR pathway.14-21 Several earlier studies have found that PLB kills prostate cancer cells and inhibits prostate cancer TAK-901 growth in tumor-bearing nude mice via ROS-mediated apoptotic pathways.22-24 Our recent quantitative proteomic study has shown that PLB upregulates and downregulates a number of functional proteins involved in cell cycle distribution apoptosis autophagy and ROS generation.25 However the molecular mechanisms for the anticancer effects of PLB on prostate cancer are not TAK-901 fully elucidated. With this study we investigated the effects of PLB within the apoptosis and autophagy in human being prostate cancer Personal computer-3 and DU145 cells and the part of Sirt1- and PI3K/Akt/mTOR-mediated pathways. Number 1 The chemical structure and cytotoxicity of PLB toward Personal computer-3 and Rabbit Polyclonal to IL4. DU145 cells. Materials and methods Chemicals and reagents 4 6 (DAPI) 5 6 7 diacetate (CM-H2DCFDA) SB202190 (4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)1H-imidazole a selective inhibitor of p38 mitogen-activated protein kinase [MAPK] used as an autophagy inducer) wortmannin (WM a potent irreversible and selective PI3K inhibitor and a blocker of autophagosome formation) phenol red-free tradition medium and fetal bovine serum (FBS) were bought from Invitrogen Inc. (Carlsbad CA USA). Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 medium were from Corning Cellgro Inc. (Herndon VA USA). PLB thiazolyl blue tetrazolium bromide (MTT) N-acetyl-L-cysteine (NAC an ROS scavenger) apocynin (Apo 4 an inhibitor of NADPH oxidase) 4 acid (HEPES) ethylenediaminetetraacetic TAK-901 acid (EDTA) and Dulbecco's phosphate buffered saline (PBS) were purchased from Sigma-Aldrich Co. (St Louis MO USA). Bafilomycin A1 (an autophagy inhibitor inhibiting fusion between autophagosomes and lysosomes) and chloroquine (an autophagy inhibitor inhibiting endosomal acidification) were purchased from Invivogen Inc. (San Diego CA USA). SRT1720 (SRT a selective Sirt1 activator N-(2-(3-(piperazin-1-ylmethyl)imidazo[2 1 phenyl)quinoxaline-2-carboxamide hydrochloride) and FK866 ((E)-N-(4-(1-benzoylpiperidin-4-yl)butyl)-3-(pyridin-3-yl) acrylamide an extremely specific non-competitive inhibitor of pre-B cell colony-enhancing aspect (PBEF)/visfatin were bought from Selleckchem Inc. (Houston TX USA). Sirtinol (STL a particular Sirt1 and Sirt2 inhibitor (E)-2-((2-hydroxynaphthalen-1-yl)methyleneamino)-N-(1-phenylethyl)benzamide) was bought from BioVision Inc. (Milpitas CA USA). Rapamycin was.
Histone modification plays a pivotal role on gene regulation as regarded
Histone modification plays a pivotal role on gene regulation as regarded as global epigenetic markers especially in tumor related genes. decreased at the transcription start site of a subset of genes. Altered H4K16ac was associated with changes in mRNA expression of the corresponding genes which were further validated in quantitative RT-PCR and western blotting assays. Our results demonstrated that "type":"entrez-nucleotide" attrs :"text":"CG200745″ term_id :"34091806″ term_text :"CG200745″CG200745 causes NSCLC cell growth inhibition through epigenetic modification of critical genes in cancer cell survival providing pivotal clues as a promising chemotherapeutics against lung cancer. Introduction Epigenetic modifications such as CpG DNA methylation or histone acetylation are regarded as an important step in cancer development and therefore have been studied to discover cancer biomarkers and therapeutic stratege [1–3]. Once cytosine methylation occurs on CpG dinucleotides via the action of DNA methyl transferase (DNMT) the methyl cytosine is maintained to the next generation due to the lack of a DNA de-methyl transferase in mammals. The irreversible histone modification has been also used as a biomarker for the early diagnosis or Temsirolimus (Torisel) prognosis of cancer as well as an effective target in cancer therapeutics [4 5 Acetylation or methylation on lysine residues of H3 and H4 Temsirolimus (Torisel) amino terminal tails are dominant histone modifications and each is responsible for the expression of bound genes. For example methylations on lysine 4 of H3 and lysine 27 of H3 are known as transcriptional activating and repressing events for histone bound genes respectively. Histone acetylation on lysine 16 of H4 is related to transcriptional activation and/or replication initiation of corresponding genes. In normal cells histone acetylation is precisely controlled by histone acetyl transferase (HAT) and histone deacetylase (HDAC). Hyper-acetylation of oncogenes or hypo-acetylation of tumor suppressor Temsirolimus (Torisel) genes however is frequently observed in various cancers. HDAC inhibitors (HDACi) are the most developed anti-cancer drugs targeting epigenetic modulation and are being applied for the treatment of various cancers particularly in solid tumors such as breast colon lung and ovarian cancers as well as in haematological tumors such as lymphoma leukemia and myeloma [6–9]. In addition epigenetic dysregulation in lung cancer is often related with the overexpression of HDAC1 and aberrant methylation of certain genes resulting in therapeutic efficacy of combination epigenetic therapy targeting DNA methylation and histone deacetylation. HDACs comprise three classes: Class I HDAC 1 2 3 and 8; Class II HDAC 4 5 6 7 9 and 10; and Class III HDAC 11 (sirtuins 1–7) [10 11 HDACi trichostatin A (TSA) [12 13 or vorinostat (SAHA)[14–16] inhibit class DLL1 I and II HDAC enzymes resulting in growth arrest apoptosis differentiation and anti-angiogenesis of cancer cells when used independently or in combination with other anti-cancer agents. Mechanistically the restoration of silenced tumor suppressor genes or suppression of activated oncogenes in cancer cells plays a critical role in the anti-cancer effects of drugs. This is followed by the induction of cell cycle arrest at the G1 stage through the expression of p21 and p27 proteins or a G2/M transition delay through the transcriptional downregulation of cyclin B1 plk1 and survivin. HDAC inhibitor "type":"entrez-nucleotide" attrs :"text":"CG200745″ term_id :"34091806″ term_text :"CG200745″CG200745 (E)-N(1)-(3-(dimethylamino)propyl)-N(8)-hydroxy-2-((naphthalene-1-loxy)methyl)oct-2-enediamide has Temsirolimus (Torisel) been recently developed and presently undergoing a phase I clinical trial. Its inhibitory effect on cell growth has been demonstrated in several types of cancer cells including prostate cancer renal cell carcinoma and RKO cells (colon carcinoma cells) in mono- and combinational-therapy with other anticancer drugs [17–19]. The mechanism underlying the cell growth inhibition of "type":"entrez-nucleotide" attrs :"text":"CG200745″ term_id :"34091806″ term_text :"CG200745″CG200745 in RKO cells has been shown to occur in a p53-dependent manner [19]. Importantly {"type":"entrez-nucleotide" attrs.
Dengue trojan (DENV) may be the leading reason behind mosquito-borne viral
Dengue trojan (DENV) may be the leading reason behind mosquito-borne viral disease and loss of life in humans. effective cell-free transmitting of viral infectivity pDC activation depends upon cell-to-cell contact an attribute observed for several cell types and principal cells contaminated by DENV aswell as Western world Nile Nelarabine (Arranon) trojan another person in the genus. We present which the sensing of DENV contaminated cells by pDCs needs viral envelope protein-dependent secretion and transmitting of viral RNA. Regularly using the cell-to-cell sensing-dependent pDC activation we discovered that DENV structural elements are clustered on the user interface between pDCs and contaminated cells. The actin cytoskeleton is normally pivotal for both this clustering on the connections and pDC activation recommending that structural network most likely plays a part in the transmitting of viral elements towards the pDCs. Because of an evolutionarily conserved suboptimal cleavage from the precursor membrane protein (prM) DENV contaminated cells launch uncleaved prM containing-immature contaminants which are lacking for membrane fusion function. We demonstrate that cells liberating immature contaminants result in pDC IFN response even more Rabbit polyclonal to CDH1. potently than cells creating fusion-competent mature disease. Altogether our outcomes imply immature contaminants like a carrier to endolysosome-localized TLR7 sensor may donate to control the development of dengue disease by eliciting a solid innate response. Writer Summary Viral reputation by the sponsor often causes an antiviral condition which suppresses viral pass on and imparts adaptive immunity. Like many infections dengue disease (DENV) defeats the host-sensing pathway within contaminated cells. However research have demonstrated an integral part of innate immunity in managing DENV disease. Here we record that sensing of DENV-infected cells by nonpermissive innate immune Nelarabine (Arranon) system cells the plasmacytoid dendritic cells (pDCs) causes a Nelarabine (Arranon) cell-contact- and TLR7-reliant activation of a solid antiviral IFN response. This cell-to-cell sensing requires transmitting of viral components that are clustered in the user interface between pDCs and contaminated cells and it is regulated from the actin network. Significantly we exposed that uncleaved prM surface area protein-containing immature contaminants play an integral function in stimulating the innate immune system response. These noninfectious immature contaminants are released Nelarabine (Arranon) by contaminated cells because of a suboptimal cleavage site which can be an evolutionarily conserved viral feature that most likely mementos the export of infectious disease by avoidance of early membrane fusion in the secretory pathway. Consequently our results Nelarabine (Arranon) focus on a conceptually book trade-off between effective infectious virus launch and the creation of IFN-inducing contaminants. This idea may have wide importance for the countless infections that like DENV can disable the pathogen-sensing equipment within contaminated cells and may launch uncleaved glycoprotein-containing noninfectious contaminants. Intro The innate disease fighting capability functions as the 1st line of protection for the sensing of viral disease. This involves fast recognition of pathogen-associated molecular patterns (PAMPs) including viral nucleic acids by pattern recognition receptors (PRRs). This recognition results in an antiviral response characterized by the production of type I interferons (IFNs) and expression of IFN-stimulated genes (ISGs). This response suppresses viral spread by blocking the viral life cycle at multiple levels and also mediates immunomodulatory effects in surrounding tissues that impart the onset of the adaptive immune response [1]. The PRR can be cytoplasmic e.g. retinoic inducible gene-I (RIG-I)-like receptors (RLRs) and NOD-like receptors (NLRs) or endosomal e.g. Toll-like receptors (TLRs) [1]. Thus depending on their intracellular localization virus-induced innate immune signaling typically occurs within cells that are either productively infected or that have internalized viral particles [1] [2]. Recent studies illustrated the existence of alternative host sensing strategies by bystander plasmacytoid dendritic cells (pDCs) which recognize infected cells [3] [4] [5] [6] [7]. pDCs are immune cells known to function as sentinels of viral infection and are a major type I IFN-producing cell type 0.41% of PBMCs (Figure 1B upper panel) are known to produce IFNα [9]. Antibody-mediated pDC depletion from PBMCs (Figure 1B middle panel) abolished IFNα secretion in response to co-culture.
Radial glia the neural stem cells of the neocortex can be
Radial glia the neural stem cells of the neocortex can be found in two niches: the ventricular area and external subventricular zone. elements potentiation of development factor indicators by extracellular matrix proteins and activation of self-renewal pathways thus allowing the developmental and evolutionary enlargement of TG101209 the individual neocortex. Graphical abstract Introduction The human neocortex contains 16 billion neurons of diverse types that develop from an in the beginning uniform neuroepithelium. In the ventricular zone (VZ) PRKD3 radial glia undergo interkinetic nuclear migration and possess apical processes that contact the ventricle and form adherens junctions. Apical complex proteins transduce signals from your cerebrospinal fluid that are critical for the survival proliferation and neurogenic capacity of ventricular radial glia (vRG) (Lehtinen et al. 2011 However the majority of human radial glia are located in the outer subventricular zone (OSVZ) (Lewitus et al. 2013 These outer radial glia (oRG) maintain basal processes but lack apical junctions and undergo a distinct migratory behavior mitotic somal translocation directly preceding cell division (Hansen et al. 2010 Thus vRG and oRG cells reside in unique niches defined by differences in anatomical location provision of growth factors cell TG101209 morphology and behavior (Fietz et al. 2010 Although oRG cells may generate TG101209 the majority of cortical neurons (Lewitus et al. 2013 Smart et al. 2002 the molecular features sustaining neural stem cell properties of oRG cells in the OSVZ market are mainly unknown and the long-term proliferative capacity of these cells has not been examined. Understanding the molecular programs specifically employed by oRG cells would provide insights into mechanisms of cortical development and support strategies to generate this cell type (Pollen et al. 2014 We find the proneural gene networks recently attributed to oRG cells are mainly restricted to intermediate progenitor cells. Within classically defined radial glia we discover molecular distinctions between vRG and oRG cells. The transcriptional state enriched in oRG cells includes genes involved in extracellular matrix production epithelial-to-mesenchymal transition and stem cell maintenance. Remarkably we find components of the LIFR/STAT3 self-renewal TG101209 pathway are selectively indicated by oRG but not vRG cells and we confirm that STAT3 signaling is necessary for oRG cell cycle progression. We further find that solitary oRG cells have the capacity to produce hundreds of deep and top cortical coating neurons. Based on these results we propose that oRG cells directly support the development of an enlarged OSVZ neural stem cell market through the local production of growth factors the manifestation of extracellular matrix proteins that potentiate growth factor signaling and the activation of the LIFR/STAT3 signaling pathway. Results Molecular Diversity of Cells in the Cortical Germinal Zones To analyze molecular features of cells in the germinal zones during human being cortical neurogenesis we captured solitary cells from microdissected VZ and SVZ specimens of human being cortex at gestational week 16-18 (GW16-18) and generated sequencing libraries (schematic Number 1A). We consequently analyzed 393 solitary cells from three individuals in which we recognized at least 1000 genes (Table S1). To classify cells we performed principal component analysis (PCA) and used expectation-maximization clustering to group cells based on their position in Personal computer space (Number S1 Experimental Methods). Based on the manifestation of known marker genes we interpreted organizations to represent cells along the cortical excitatory lineage and inhibitory interneurons generated in the ventral telencephalon (Numbers 1B 1 1 and S1 Table S2). Number 1 Molecular Diversity of Solitary Cells from Human being Cortical Germinal Zone We further examined groups of cells expressing known markers of the cortical excitatory neuron lineage (schematic Number 2A). Four organizations robustly indicated markers of human being radial glia (yellow bar Number 2A). Another four organizations retained a reduced level of and manifestation but also.
To regulate how tetraspanin KAI1/CD82 a tumor metastasis suppressor inhibits cell
To regulate how tetraspanin KAI1/CD82 a tumor metastasis suppressor inhibits cell migration we assessed which cellular events critical for motility are altered by KAI1/CD82 and how KAI1/CD82 regulates these events. events was caused by poor formations of actin cortical network and stress fiber and by aberrant dynamics in actin organization. Rac1 activity was reduced by KAI1/CD82 consistent with the diminution of lamellipodia and actin cortical network; while the growth factor-stimulated RhoA activity was blocked by KAI1/CD82 consistent with the loss of stress fiber and attenuation in cellular retraction. Upon KAI1/CD82 expression Rac effector cofilin was not enriched at the cell periphery to facilitate lamellipodia formation while Rho kinase exhibited a significantly lower activity leading to less retraction. Phosphatidylinositol 4 5 which initiates actin polymerization from the plasma membrane became Cdc14B2 less detectable at the cell periphery in KAI1/CD82-expressing cells. Moreover KAI1/CD82-induced phenotypes likely resulted from the suppression of multiple signaling pathways such as integrin and growth factor signaling. In summary at the cellular level KAI1/CD82 inhibited polarized protrusion and retraction events by disrupting actin reorganization; at the molecular level KAI1/CD82 deregulated Rac1 RhoA and their effectors cofilin and Rho kinase by perturbing the plasma membrane lipids. Introduction Regulating cell motility is a common feature of many tetraspanins [1]-[4]. Although it remains largely unclear how tetraspanins modulate cell motility possible mechanisms Tubastatin A HCl have started to emerge from recent research [5]-[7]. Lines of evidence suggest that tetraspanins could regulate the functional status of cell adhesion molecules and growth factor receptors (or membrane-bound growth factor) that they physically and/or functionally associate with and then alter cellular behaviors through these partners [1]-[7]. For example KAI1/CD82 attenuates epidermal growth factor (EGF) signaling and integrin function by accelerating endocytosis of its associated EGF receptor and integrin respectively [8] [9]. In parallel experimental data also support the notion that tetraspanins solicit outside-in signals to modulate cellular functions [1]-[4]. Again taking KAI1/CD82 as an example immuno-crosslinking of cell surface KAI1/CD82 demonstrates that KAI1/CD82 functions as a costimulatory molecule during T cell activation [10]-[15] indicating that KAI1/CD82 plays a direct role in signal initiation and/or transduction. Regardless of which of the two possible mechanisms plays a far more predominant part tetraspanins and/or their connected molecules must work on cytoskeleton to improve motility-related mobile events and eventually influence cell motility. For instance clustering the cell surface area KAI1/Compact disc82 proteins through the use of immobilized KAI1/Compact disc82 monoclonal antibody (mAb) induces profound dendritic mobile procedures in T cells followed from the rearrangement of actin cytoskeleton and the bond of KAI1/Compact disc82 to actin cytoskeleton inside a protein kinase A activity-dependent but Src kinase activity-independent way [12] [15]. Further research possess indicated that Rho little GTPases are necessary for KAI1/Compact disc82-induced dendritic procedures in T cells [13]. Cell migration needs the polarized development and expansion of Tubastatin A HCl mobile protrusions the transmembrane connection of cytoskeleton to extracellular matrix (ECM) to create extender to propel the cell body ahead as well as the retraction of the trunk mobile portion [16]. Therefore naturally cell migration is usually a process of global reorganization of cytoskeleton. For example actin polymerization drives the formation and extension of Tubastatin A HCl the protrusions such as lamellipodia at the leading edge [17]-[20] while the asymmetric distribution and enzymatic engagement of myosin and actin produce the force for cellular contractility and lead to the retraction of the trailing edge [21]-[23]. Rho small GTPases are clearly pivotal in all of these cytoskeletal rearrangement processes [16]. For Tubastatin A HCl instance Rac is mainly responsible for producing a protrusive power through the localized actin polymerization while Rho is in charge of the contraction from the cell body as well as the retraction of the trunk end [24]. As downstream effectors of Rho GTPases [16] cofilin severs actin filament to create barbed ends and therefore facilitates the actin treadmilling [16] [25] while Arp2/3 complicated nucleates Tubastatin A HCl brand-new actin filaments through the edges of preexisting filaments [16] [26]. The severing activity of cofilin and branching activity of Arp2/3 function coordinately to market the forming of a branched actin network or cortical actin meshwork on the.
The Epstein-Barr virus (EBV) encoded oncoprotein Latent Membrane Proteins 1 (LMP1)
The Epstein-Barr virus (EBV) encoded oncoprotein Latent Membrane Proteins 1 (LMP1) signals through two C-terminal tail domains to operate a vehicle cell growth survival and transformation. improved LMP1 TES1 domain-mediated activation Rabbit polyclonal to ZNF300. from the p38 JNK ERK and canonical NF-kB pathways however not non-canonical GBR-12935 2HCl NF-kB pathway activity. To get insights into how TRAF1 amplifies LMP1 TES1 MAP kinase and canonical NF-kB pathways we performed proteomic evaluation of TRAF1 complexes immuno-purified from cells uninduced or induced for LMP1 TES1 signaling. Unexpectedly we discovered that LMP1 TES1 website signaling induced an association between TRAF1 and the linear ubiquitin chain assembly complex (LUBAC) and stimulated linear (M1)-linked polyubiquitin chain attachment to TRAF1 complexes. LMP1 or TRAF1 complexes isolated from EBV-transformed lymphoblastoid B cell lines (LCLs) were highly altered by M1-linked polyubiqutin chains. The M1-ubiquitin binding proteins IKK-gamma/NEMO ABIN1 and A20 each associate with TRAF1 in cells that express LMP1. TRAF2 however not the cIAP1 or cIAP2 ubiquitin ligases has an integral function in LUBAC recruitment and M1-string connection to TRAF1 complexes implicating the TRAF1:TRAF2 heterotrimer in LMP1 TES1-reliant LUBAC activation. Depletion of either TRAF1 or the LUBAC ubiquitin E3 ligase subunit HOIP markedly impaired LCL development. Furthermore LMP1 or TRAF1 complexes purified from LCLs had been embellished by lysine 63 (K63)-connected polyubiqutin chains. LMP1 TES1 signaling induced K63-polyubiquitin string attachment to TRAF1 TRAF2 and complexes was defined as K63-Ub string focus on. Co-localization of M1- and K63-linked polyubiquitin chains on LMP1 complexes may facilitate downstream canonical NF-kB pathway activation. Our results showcase LUBAC being a book potential therapeutic focus on in EBV-associated lymphoproliferative disorders. Writer Overview The linear ubiquitin set up complex (LUBAC) has crucial assignments in immune system receptor-mediated NF-kB and MAP kinase pathway activation. Relatively little is well known about the level to which GBR-12935 2HCl microbial pathogens make use of LUBAC to activate downstream pathways. We demonstrate that TRAF1 enhances EBV oncoprotein LMP1 TES1/CTAR1 domains mediated MAP kinase and canonical NF-kB activation. LMP1 TES1 signaling induces association between LUBAC and TRAF1 and sets off M1-polyubiquitin string attachment to TRAF1 complexes. TRAF1 and LMP1 complexes are embellished by M1-polyubiquitin chains in LCL ingredients. TRAF2 has an integral function in LMP1-induced LUBAC recruitment and M1-string connection to TRAF1 GBR-12935 2HCl complexes. TRAF1 and LMP1 complexes are improved by lysine 63-connected polyubiquitin chains in LCL ingredients and TRAF2 is normally a focus on of LMP1-induced GBR-12935 2HCl K63-ubiquitin string attachment. The TRAF1:TRAF2 heterotrimer may coordinate ubiquitin signaling downstream of TES1 Thus. Depletion of TRAF1 or the LUBAC subunit HOIP impairs LCL success and development. Hence although TRAF1 may be the just TRAF with out a Band finger ubiquitin ligase domains TRAF1 nonetheless provides important assignments in ubiqutin-mediated indication transduction downstream of LMP1. Our function shows that LUBAC is normally very important to EBV-driven B-cell proliferation and shows that LUBAC could be a book therapeutic focus on in EBV-associated lymphoproliferative disorders. Launch Epstein-Barr trojan (EBV) can be an oncogenic gamma-herpesvirus this is the causative agent of infectious mononucleosis. While EBV an infection generally leads to subclinical lifelong an infection for most people EBV is normally nonetheless connected with multiple individual malignancies [1 2 3 4 5 Included in these are Hodgkin lymphoma post-transplant lymphoproliferative disease (PTLD) and HIV-associated lymphomas. In these malignancies the main EBV oncoprotein Latent Membrane Proteins 1 (LMP1) is definitely often indicated. LMP1 constitutively activates growth and survival pathways by mimicking CD40 signaling [6 7 8 CD40 is definitely a member of the tumor necrosis element receptor (TNFR) family and serves as a key B-cell costimulatory molecule [9 10 11 LMP1 manifestation transforms rodent fibroblasts and murine B-cells and is necessary for EBV-mediated conversion of human being B lymphocytes into immortalized lymphoblastoid cell lines (LCLs) [12 13 14 15 16 17 LMP1 is definitely comprised of a 24-residue N-terminal cytoplasmic tail 6 transmembrane domains (TM) and a 200 residue C-terminal cytoplasmic tail. Deletion of the LMP1 N-terminus abrogates EBV-mediated B-cell transformation and alters LMP1 localization [18]. Particular roles from the LMP1 However.
Direct cell-cell spread of Human Immunodeficiency Virus type-1 (HIV-1) at the
Direct cell-cell spread of Human Immunodeficiency Virus type-1 (HIV-1) at the virological synapse (VS) is an efficient mode of dissemination between CD4+ T cells but the mechanisms by which HIV-1 proteins are directed towards intercellular contacts is usually unclear. the HIV-1-infected T cell towards engaged target T cell concomitant with polarization of viral proteins. Directed secretion of proteins at the T cell Is usually requires specialized organelles termed secretory lysosomes (SL) and we show that this HIV-1 envelope glycoprotein (Env) localizes with CTLA-4 and FasL in SL-related compartments and at the VS. Finally CD4+ T cells that are disabled for controlled secretion are less able to support effective cell-to-cell HIV-1 spread. We propose that Rabbit Polyclonal to OR4A15. HIV-1 hijacks the controlled secretory pathway of CD4+ T cells to enhance its dissemination. Author Summary Human being Immunodeficiency Computer virus type-1 (HIV-1) the cause of the global AIDS pandemic CP-724714 mainly infects Compact disc4+ T lymphocytes and effective replication necessitates transmitting of an infection to prone neighbouring cells. To make sure that the necessary elements are carried to the right area in the cell with the right period viral assembly is normally carefully controlled. To get this done HIV-1 preferentially assembles and buds at sites of cell-cell get in touch with known as virological synapses. T lymphocytes hire a specific trafficking pathway referred to as the governed secretory pathway that's activated pursuing cell-cell contact. Right here we present that HIV-1 proteins associate with mobile compartments that get excited about governed secretion which Compact disc4+ T cells from sufferers with a hereditary defect within this pathway are much less in a position to support dispersing an infection. We conclude that HIV-1 hijacks components of the T cell governed secretory pathway to market T cell-to-T cell transmitting at virological synapses. This research provides the initial proof that lymphotropic infections can hijack governed secretion to improve pathogenesis and could open up brand-new opportunities for logical drug design to greatly help fight HIV/AIDS. Launch Viral replication is normally a complex group of well-orchestrated occasions culminating in the discharge of progeny virions from contaminated cells. For effective viral creation all components should be mobilized to the website of CP-724714 assembly within a coordinated way attained by enlisting the different parts of mobile transport pathways. It really is becoming increasingly apparent that cell-to-cell pass on is an essential system of viral dissemination [1] and includes a number of advantages of viruses including faster and effective uptake by permissive focus on cells. However this technique requires a perfect level of legislation to polarize trojan assembly and discharge towards engaged focus on cells at sites of cell-cell get in touch with. Direct pass on of Individual Immunodeficiency Trojan type-1 (HIV-1) between Compact disc4+ T cells occurs across a supramolecular framework called the "virological synapse" (VS) [2]. Another mode of HIV-1 spread between immune cells is definitely via membrane nanotubes [3] although these constructions form less regularly than VS and so probably contribute less to viral dissemination [3] [4]. The VS was so named because of structural and practical similarity to the immunological synapse (Is definitely) that evolves between an antigen showing cell and a T cell [5]. The VS is definitely characterised by quick actin-mediated recruitment of the HIV-1 access receptors (CD4 and a chemokine receptor) and adhesion molecules on the prospective cell synchronous with polarization of the HIV-1 envelope glycoprotein (Env) the Gag polyprotein and integrins in the infected cell. Within the HIV-1+ infected cell Env and Gag polarization is definitely actin- and tubulin-dependent and requires lipid raft CP-724714 integrity [6] [7] [8] implying that viral proteins are actively recruited to the site of cell-cell contact. CP-724714 Indeed live cell imaging visualizing disease assembly in the VS has shown recruitment of Gag to sites of cell-cell contact [9]. By contrast the pathway of Env trafficking in infected T cells and the molecular mechanisms underlying active Env enrichment in the VS remain poorly understood. Once in the VS cell-to-cell spread of HIV-1 is definitely by polarized assembly and budding of virions into the synaptic cleft and subsequent fusion with the prospective cell plasma membrane either in the cell surface or from within an endosomal compartment [9] [10]. Polarized secretion in the Is definitely has been well analyzed in CD8+ T cells and is directed by antigen-dependent reorientation of the CP-724714 microtubule organizing center (MTOC) to sites of cell-cell contact and the.
T cells recognize and wipe out an array of pathogen-infected or
T cells recognize and wipe out an array of pathogen-infected or cancers cells utilizing a diverse group of T cell receptors (TCR). individual bloodstream. We demonstrate which the repertoire of principal antigen-specific T cells from pathogen inexperienced people has a amazingly broad affinity selection of 1000-fold made up of different TCR sequences. Within this range examples from older people contained a lower life expectancy regularity of high affinity T cells in comparison to youthful people demonstrating an age-related aftereffect of T cell attrition that might lead to openings in the repertoire. iTAST should enable the speedy collection of high affinity TCRs ex girlfriend or boyfriend vivo for adoptive immunotherapy and dimension of T cell response for immune system monitoring applications. Launch Compact disc8+ T lymphocytes certainly are a subclass of T cells JNJ 26854165 that straight kills cancers and pathogen-infected cells through identification of peptide destined to main histocompatibility complicated (pMHC) which consists of TCR (1). The affinity of the TCR to confirmed peptide epitope would depend on its TCR series which influences the downstream destiny (2) JNJ 26854165 and useful capability (3) of T cells by modulating TCR signaling power (4) and proliferation prices (3 5 TCR-pMHC affinity is normally widely known to be a major determinant in the effectiveness of adoptive T cell transfer therapy (Take action). Thus ability to track TCR-pMHC affinity of solitary antigen-specific T cells within humans can provide important information on JNJ 26854165 the quality of an immune response and for selecting the optimum T cells for Take action immunotherapy in malignancy (6) and prolonged viral infections (7 8 Measurement of TCR properties is definitely inherently hard because each T cell consists of its own unique TCR that can recognize a distinct set of pMHC ligands. The "gold standard" for measuring TCR-pMHC affinity is definitely Surface Plasmon Resonance (SPR) which requires the production of recombinant soluble TCR. The polyclonal nature of T cells makes SPR measurement laborious and low-throughput incredibly. Many solutions to measure TCR-pMHC affinity and kinetics from live T cells possess been recently established. Fluorescence microscopy-based JNJ 26854165 assays can gauge the TCR-pMHC dissociation price of soluble pMHCs destined to the T cell (7). TCR-pMHC 2-dimensional (2D) kinetics and affinity are also measured this way utilizing a fluorescence resonance energy transfer (FRET) program (9). The throughput of the methods is bound with the field of watch. In addition a lot more than 104 antigen-specific T cells should be put into the chamber to be able to gauge the kinetics of ~50 cells which isn't usually accessible in a single individual blood pull (7). The micropipette adhesion regularity assay is normally another method that may measure 2D TCR-pMHC kinetics and affinity but without requirements on cell insight count (5). Nevertheless this assay isn't suitable for measure 2D Snca affinities on principal T cells as the regularity of antigen-specific T cells have become low specifically precursor cells in antigen inexperienced people and there's a high amount of inefficiency because of period spent on nonreactive T cells. It has limited its make use of to either mouse types of an infection (10) T cell clones or TCR transgenic mouse systems (5 11 T cell extension right into a monoclonal people has its disadvantages; aside from the period and labor connected with extension the causing T cell clones may not represent the beginning principal T cell people because each T cell provides different proliferative potential (12). Furthermore none of the methods can simply hyperlink TCR-pMHC binding variables to TCR series which provides details on T cell clonal extension and lineage (13). Right here we present iTAST that allows dimension of single-cell 2D TCR affinity and series straight from primary Compact disc8+ T cells extracted from one individual blood attracts at a throughput as high as ~75 cells each day. We present that iTAST has an accurate evaluation of TCR affinity on the one cell level that highly correlates with TCR affinity by SPR typical 2D affinity (5) and cell useful capacity. We used iTAST to review the na?ve repertoire of HCV-specific Compact disc8+ T cells within healthful individuals and uncovered a broad TCR affinity range that's reliant on age. The capability to get correlated TCR affinity and series details generated by iTAST should enable the speedy collection of high affinity TCRs for adoptive immunotherapy. Outcomes Summary of iTAST iTAST uses streptamers (14) which really is a pMHC multimer that can reversibly label antigen-specific.
Based on research in yeast and mammalian cells the Elongator complex
Based on research in yeast and mammalian cells the Elongator complex continues to be implicated in features as diverse as histone acetylation polarized protein trafficking and tRNA modification. of complementation is because of incompatibility with fungus as coexpression of both seed genes within an fungus mutant restored Elongator's tRNA adjustment function also backed partial complementation by yeast-plant Elp3 hybrids recommending that AtElp1 provides less stringent series requirements for Elp3 than ScElp1. We conclude that fungus and seed Vandetanib (ZD6474) Elongator talk about tRNA adjustment roles and suggest that this function may be conserved in Elongator from all eukaryotic kingdoms of lifestyle. Introduction Research in areas as disparate as leaf advancement (Nelissen to delicate to a toxin (zymocin) secreted by (evaluated in Schaffrath and Breunig 2000 and qualified prospects to the recognition of genes inside a display for zymocin-resistant (to determined the homologue of candida can be homologous to to (Nelissen (and displays series similarity to candida (Creppe Elongator insufficiency was also connected with neurological and developmental defects and tRNA changes defects (Chen mutants still consist of acetylated α-tubulin although the particular level may be decreased (Chen mutant tRNA wobble uridine adjustments including mcm5s2U are jeopardized. By complementing candida mutants with genes we demonstrate how the candida subunits can assemble with vegetable polypeptides to create hybrid Elongator complexes indicating high structural similarity between candida and vegetable Elongator. Strikingly even though AtElp3/ELO3 probably the most conserved subunit could structurally change candida Elp3 practical complementation with had not Vandetanib (ZD6474) been noticed unless was concurrently replaced by genome contains only one homologue for every from the candida genes and proof for an identical hexameric complicated made up of two subcomplexes was recently obtained by tandem affinity purification (Nelissen mutant strains with the corresponding plant cDNAs fused to a yeast promoter. Sensitivity to γ-toxin the active component of the killer toxin zymocin provided a sensitive assay for Elongator function. If the heterologous protein integrated into the yeast Elongator complex and functioned in restoring tRNA modification we expected reversion of the toxin resistance phenotype of the Elongator mutant. Because the AtELP3 subunit is most similar to its yeast homologue we first tried to complement the mutant. A c-myc-tagged version of the AtELP3 protein could be produced at levels comparable to those of yeast Elp3-c-myc (see below) but the toxin resistance of the yeast gene (not shown) or an untagged allele (Fig. 1A). Likewise thermosensitivity and hypersensitivity to caffeine Vandetanib (ZD6474) additional phenotypes of Elongator mutants were not altered from the vegetable gene (Fig. 1B). Reintroduction from the candida gene in to the to check the (pFF9) (YEpA4) and … Vandetanib (ZD6474) The failing of vegetable AtELP3 to replacement for candida Elp3 function had not been because of instability from the protein as AtELP3-c-myc was precipitated from total candida protein components at levels much like Elp3-c-myc (Fig. 1C best -panel). c-myc-tagged Elp3 and AtElp3 both could co-precipitate the biggest Elongator subunit Elp1 indicating discussion between AtELP3 and Elp1 (Fig. 1C bottom level -panel). To analyse whether AtELP3-Elp1 discussion occurred in Vandetanib (ZD6474) the framework from the Elongator complicated we used the actual fact that discussion between the subunits Elp5 and Elp2 depends on the structural integrity from the complicated and the current presence of Elp3 (Frohloff gene on the plasmid (Fig. 2A lane 3). When or alleles were introduced of cDNA clone instead. Consistent with earlier reviews (Frohloff gene restored Elp3-HA balance and discussion between Elp2 and Elp3 (Fig. 3A lane 4). Incredibly the same kept accurate when was released (Fig. 3A lane 3). Manifestation from the vegetable gene from the inducible promoter in the expression also restored the interaction Rabbit Polyclonal to NDUFA9. between Elp2 and Kti12 (Fig. 3B). Fig. 3 Restoration of Elp3 stability and Elongator subunit interactions in an mutant the efficiency of subunit interactions was somewhat reduced Vandetanib (ZD6474) compared with that of the transformants. Nonetheless our data show that AtELP1 like AtELP3 are assembled into complexes where they are able to structurally replace the respective yeast Elongator subunits. Together plant AtELP1 and AtELP3 support tRNA modification in yeast Because Elp3 requires Elp1 for stability its function may depend on specific contacts between these two proteins explaining the failure of.
Human blood Vγ9/Vδ2 T cells monocytes and neutrophils share a responsiveness
Human blood Vγ9/Vδ2 T cells monocytes and neutrophils share a responsiveness toward inflammatory chemokines and are rapidly recruited to Cefdinir Cefdinir sites of infection. necrosis factor (TNF)-α. This response is dependent on the ability of these bacteria to produce the microbial metabolite (specialized T cell subsets feedback to innate cells [2]. Among these interactions the regulation of neutrophil-mediated inflammatory responses by Th17 cells has received enormous attention over the past few years [3] and with the emergence of novel T cell subsets additional networks are being proposed so that each polarized T cell eventually pairs with an innate counter player [4]-[7]. The necessity to integrate complex signals in order to mount the most effective defense is best illustrated by the Rabbit Polyclonal to CD70. existence of 'unconventional' T cells bridging the classical divide between innate and adaptive immunity such as natural killer T cells mucosal-associated invariant T cells intestinal intraepithelial CD8αα+ T cells and dendritic epidermal γδ T cells [8]-[14]. These often tissue-associated lymphocytes are characterised by restricted T cell receptor (TCR) repertoires that allow them to respond rapidly to a restricted selection of conserved buildings. Unconventional T cells easily assume various effector functions which range from sentinel duties and targeted eliminating to participating with keratinocytes fibroblasts phagocytes and antigen-presenting cells and also other lymphocyte. γδ T cells expressing a Vγ9/Vδ2 TCR – Vγ2/Vδ2 regarding to an alternative solution nomenclature – are just found in human beings and higher primates and differ fundamentally from all the regular and unconventional T cells [15]. Activated Vγ9/Vδ2 T cells create a selection of cytokines eliminate infected and changed focus on cells regulate success and differentiation of monocytes and maturation of dendritic cells offer B cell help and present antigens to Compact disc4+ and Compact disc8+ T cells [11] [12] [16] [17]. They broaden considerably in lots of infections sometimes to >50% of most circulating T cells in a few days [18] and respond selectively within a non-MHC limited manner towards the microbial metabolite (germline-encoded design reputation receptors but requires Cefdinir the re-arranged Vγ9/Vδ2 TCR [20]-[22]. Bacterias that contain the non-mevalonate pathway and therefore generate HMB-PP comprise some of the most harmful human pathogens like the causative agencies of cholera diphtheria plague tuberculosis and typhoid but also many commensal and opportunistic types in the mucosal flora epidermis and feces [19] [23]. In every these micro-organisms HMB-PP can be an important intracellular metabolite which is not yet determined whether and exactly how it really is released by invading bacterias and becomes noticeable to the disease fighting capability as soluble molecule. Indeed earlier studies with mycobacteria suggested that uptake of whole bacteria by monocytes macrophages or DCs may be required for the recognition by Vγ9/Vδ2 T cells [24]-[27]. Neutrophils are the first immune cells infiltrating the site of contamination Cefdinir and the main phagocytes responsible for early pathogen clearance and growing evidence points toward a crucial role of γδ T cells in regulating neutrophil responses in mouse models of contamination hypersensitivity and autoimmunity [8] [12]. Yet the interplay between γδ T cells and neutrophils has not been addressed in detail [28] [29]. Our present data demonstrate that Vγ9/Vδ2 T cells Cefdinir readily respond to neutrophils harboring phagocytosed bacteria and that this response is strictly dependent on the ability of these bacteria to produce HMB-PP and cell-cell contact of Vγ9/Vδ2 T cells with accessory monocytes. The majority of circulating Vγ9/Vδ2 T cells shows migration properties similar to monocytes [30] suggesting that these two cell types are co-recruited to the site of inflammation and interact with each other at early stages of contamination [17] [31]. Our present findings thus indicate a direct link between invading pathogens neutrophils monocytes and microbe-responsive γδ T cells and suggest novel diagnostic and therapeutic approaches in acute contamination. Results Human γδ T cells induce neutrophil survival and activation Neutrophils are.

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Justice Now
All Day
Carbon offsets are a small but meaningful market in its mission to contribute to greenhouse gas reducing industries and practices in order to compensate for emissions made elsewhere.
Black History Month's theme for 2020 is African Americans and the Vote. Three Penn scholars define what the "Black vote" means when viewed through history, and what it doesn't mean when viewed as an indivisible bloc.
Engineering student Evan Weinstein fixated on the idea of liberating bespoke chocolates from the confines of both the bar and the mold. Rather than cast a chocolate shape, why not build it? Cocoa Press is his solution.
During each presidential debate, the team at FactCheck.org watches and listens closely to verify statements made by candidates, and draws precise lines between fact, misleading information, and sometimes pure fiction, for voters to have access to the truth.
Amy Gutmann cast her ballot at Vance Hall two hours shy of polls closing in this off-year election, with new voting machines on site and across the entire city.
At the University Council meeting on Oct. 23, Craig Carnaroli and Anne Papageorge highlighted the current successes and future plans of Penn's Climate and Sustainability Action Plan 3.0. with an ambitious goal—to be 100% carbon neutral by 2042.
Penn Leads the Vote, the Netter Center for Community Partnerships, and the Office of Government and Community Affairs work in tandem to make Penn a civic-minded population of voters in all elections.
High school girls who take part in the Penn Academy for Reproductive Science get a hands-on lab course with top epigenetic and reproductive health experts.
Many religious movements started off as fringe groups, and many modern-day cults have no religious doctrine. Why are cults and new religious movements conflated, and what makes them different?
On Aug. 21, the newest cohort of Quakers arrived on campus. Of all 2,400 incoming first-year students, nearly one-third arrived for Penn's official Move-In day.


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July 2020
This flyer highlights key Psychological First Aid concepts and resources disaster responders and outreach workers may use when assisting individuals affected by the pandemic. It also provides resources for further assistance.
July 2019
This Joint SAMHSA-CMS informational Bulletin provides guidance to states and school systems about addressing mental health and substance use issues in schools. It provides examples of approaches for services in schools and describes some Medicaid authorities that states may use to cover services.
April 2016
Lists questions consumers can ask themselves to help them decide whether to seek help for a substance abuse problem, a mental health issue, or both. Urges those who answered "yes" to any of the questions to seek help and lists resources for more information.
October 2015
This guide educates substance use counselors and clinicians about detoxification and withdrawal. It addresses patient placement, detox services for specific substances, co-occurring disorders, financing issues, and screening and assessment tools.
This brochure lists questions to ask yourself to help decide whether to seek help for mental illness, substance use disorders, or both. It urges people who answer "yes" to any of the questions to seek help and provides resources for more information.
Contiene preguntas que los consumidores pueden hacerse para ayudarles a decidir si se debe buscar ayuda para un problema de abuso de drogas, de salud mental, o ambas cosas. Urge a los que respondieron "sí" a cualquiera de las preguntas, que busquen ayuda y contiene una lista de recursos dónde pueden obtener más información.
This guide provides strategies for clinicians who provide substance use disorder treatment to people who have experienced child abuse and child neglect. It lists symptoms of child abuse and neglect and presents screening questions, behavioral clues, and the role of child protective services.
August 2015
Provee una visión general del tratamiento del abuso de sustancias en relación con el sistema de justicia penal. Incluye P&R relacionadas con consejería, manejo del enojo y alternativas para la encarcelación. Incluye una guía de recursos para el tratamiento del abuso de sustancias, consejería y grupos de autoayuda.
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Definition of loner
1 : one that avoids others: such as
a : a person who is often alone or likes to be alone : someone who usually avoids the company of others He is a moody loner who doesn't become chummy with anyone.— Sam Moses Many rowers will agree that sculling is a sport for loners who relish the more quixotic elements of the sport.— D. C. Churbuck … she found that the image of the scientist as an antisocial loner is a myth …— Warren E. Leary
b : a typically solitary animal Black bears are loners and usually wander by themselves in search of food.— Mark Carwardine Despite the fact they are loners, cats do have a well-organized social system based on scent marks and mutual avoidance.— Fiona Sunquist
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Mr. Govind Ballabh Pant, Union Home Minister, speaking at a tribal residential school in Coonor, near Ootacamund on May 31, said that the Adivasis had been neglected in the past and therefore all efforts should be directed towards rehabilitating them socially and economically. Mr. Pant said that work for the betterment of the Adivasis should be given national importance and that their level had to be raised. In the past they had been neglected in every sphere. They owed allegiance to Gandhiji, who not only gave lead in the political field, but also in the social and cultural life. Therefore, each one owed a duty to tribal people for their social and cultural uplift.
"I want people who can dedicate themselves to the work of uplift of Adivasis. I prefer the Ashram type of schools because in such schools, workers dedicated to the cause can be trained," Mr. Pant said. He pointed out that whatever they might do for economic development of Adivasis, they should take care not to disturb their culture and way of life. 'Let people go among them as servants and well-wishers, but not as patrons or men with some high moral order."


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Saturday I did some extra work on a movie called "Impossible Choice" (Media Artists Inc) that is being filmed in Syracuse, NY. I got this one thru AMS. Sunday I was a Day Player in a web series episode of "This is Not a Vlog" by -V Productions. Pics & recaps can be see on WWJ FACEBOOK PAGE
3/26/2012-ACTING-"Impossible Choice" & "This is Not a Vlog"
This entry was posted in ACTING, FILMS, WEB SERIES / INTERNET and tagged chris vincelette, Impossible Choice, media artists inc, ronald marquisee, this is not a Vlog, V Productions, wayne w johnson, WWJ. Bookmark the permalink.


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I have used RoadRunner Moving for 5 moves and each time is professional and very well done. Today's move was quick and efficient. My brand new carpets were covered up and furniture moved around until I was happy. The guys were very professional and very caring. Excellent customer service provided and attitude among the entire group was great. I'll surely recommend you always.
2/17/2020Roy Schwartz
We are very thankful for the amazing customer service and hard work. We had a three man crew, They were on-time, courteous, helpful and their estimate of time was almost exactly dead-on! This is our second time using RoadRunner Moving and we highly recommend it to anyone looking for a hassle-free move to help ease the stress.
6/30/2020Chuck Nelson
During my move, when I look for movers, I found RoadRunner Moving. I was relieved to see that the price never changed from last time. I called them and got a reasonable quote for move. I had a great time with the company again. I highly recommend them for peace of mind. They are reliable and truthful in their pricing. Thank you, RoadRunner Moving, for your fantastic service.
- Angela Desosa
Great service from the team today for our move. Was really easy to book by phone and they were able to accommodate my move first thing in the morning. Finishing up roughly in the time frame we had expected from the quote. I am very happy!
1/20/2020Neil Henderson
All were very professional and easy to work with. They followed up and made sure I'm happy with their service. The move to the new location was timely. They unpacked the truck and placed everything was placed exactly where we asked. Flawless and highly recommended!
5/28/2020David Barrios
The RoadRunner Moving were great in all aspects. They were great with me throughout the move. The quote was reasonable and they even ask me to look for better quote options if I want to but I was satisfied with their work. They actually were the cheapest ones. I feel like it was indeed my best move ever.
3/16/2020Robert Novack
They moved for us yesterday and everything was flawless. They are friendly and personable, prompt and hard-working. They even called us the evening before to remind us to change our clocks because of daylight savings time. Seriously, who does that? I hate moving, and don't want to do it any time again soon, but if I had to do it, I'd definitely go back to RoadRunner Moving
- Howard Shrader
Each person we dealt with from the office to the movers was extremely helpful and professional. We had tight spaces, doorways and stairs. They unhinged our doors and move our furnishings quickly with no damage. Seriously I cannot recommend this company and Jamie and team enough. If you're planning a move do not look any further than RoadRunner Moving.
5/18/2020Colleen Lange
Best movers in my life! So nice and strong and efficient. I thought the move will take about 5 hours. It took only 3 hours. Should I say I was pleasantly surprised? If I ever move again, I hope not though, I am moving with Roadrunner Moving for sure.
6/28/2019William Barrett
RoadRunner Moving are unique in every possible way. You can trust them for efficient moving. They ensured all was perfect right from the start until the end. I loved the way they were an able to manage their time efficiently and perfectly. Their services were great, and I did not have to worry about anything. Highly recommended to everyone for the move.
2/15/2020Charles Vines
Had a fantastic experience with this company. When I called to make the reservation, Michael was very helpful and friendly and answered all of my questions. Richard and Joseph were efficient and careful. They packed our TVs, large furniture, and fragile items very carefully and got everything on the truck quick. I would definitely use this company again.
Connect with our Team: (510) 640-8300
Serving Oakland and the Surrounding Areas
Oakland Zip Code(s)
94502 94601 94602 94603 94604 94605 94606 94607 94608 94609 94610 94611 94612 94613 94614 94618 94619 94620 94621 94622 94623 94624 94625 94649 94659 94660 94661 94666 94704 94705
Nearby Oakland Neighborhoods
Common topics among people researching
Local Movers in Oakland
Local packing services
For local moves you normally start by paying by the hour for the loading and packing services.
Calgary movers charge a local move by the rates vary according to Oakland of residence i.e.
Short distance movers near me in Oakland
You could fall in love with local moving just because of the way it can be very affordable.
Some renters and homeowners choose to undertake local moves on their own because of the short amount of distance involved.
Some people prefer to rent box trucks for local moves and try to take on the move alone.
Moving locally cost in Oakland
The other option that you have is to go and hire local movers who will all of those things for you.
Not all local movers are reputable and trustworthy - there are also bad moving companies that will not hesitate to use fraudulent techniques to get more money from their customers.
Local moving prices
One of the main factors to consider is eligibility of the business for local and interstate moves depending on the need.
Some local moving companies charge extra for flight charges and long carry charges as well as appliance charges.
While you shouldn't experience any issues with these partner companies since there's a direct relationship between the national brand and the local moving company it's worth asking about when you get a quote.
Most local moving companies will gladly offer you the extra service of packing and unpacking your household items.
Cheap moving help
A local moving supply store will be able to sell these to you at a reasonable price.
These extra services could be exactly what you need to make your next local move as smooth and stress-free as possible.
Van line websites will have a space to enter your zip code and will pull up local movers that you can investigate independently.
Professional movers near me in Oakland
Van lines are large companies that contract with local moving companies to transport your stuff over state lines.
Then shortlist the local moving companies with good service history and get the physical address of at least three movers who would be having real offices.
The first order of business of course will be to find some local movers to pack you up and move you.
Local professional movers
Put your relocation in the hands of the local movers northern va locals trust with their property and possessions.
Sometimes local movers charge additional fees and you should ask in advance about them to make sure the estimate you get is as close to the final movers cost as possible.
Cost of movers local
You can rent your own truck and then hire local movers to load and unload it for you.
Organizing and executing a local move by yourself is a good option if you can spare the time.
Connect with our Team: (510) 640-8300
If you plan to move to Oakland,
here are a few places that may interest you
(and if you are leaving the area, it's still not late to visit them!)
|Islamic Cultural Center of Northern California
1433 Madison St, Oakland, CA 94612
(0.40 miles)
|Camron-Stanford House
1418 Lakeside Dr, Oakland, CA 94612
(0.51 miles)
|Rotary Nature Center
City government office
500 Bellevue Ave, Oakland, CA 94610
(0.83 miles)
|The Museum of Art and Digital Entertainment
3400 Broadway, Oakland, CA 94611
(1.34 miles)
|Piedmont Funeral Services and Mountain View Cemetery
5000 Piedmont Ave, Oakland, CA 94611
(2.41 miles)
|Union Point Park
2311 Embarcadero, Oakland, CA 94606
(2.49 miles)
|Rock Wall Wine Company
2301 Monarch St, Alameda, CA 94501
(2.54 miles)
Connect with our Team: (510) 640-8300
Rsources and Links
We Accept
Main Location
RoadRunner Polaris
Union City, CA 94587


text:
december 2018
Privacy policy COOLTECH
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We will be glad to inform you about interesting topics.


text:
A Week To Go
Get ready to experience Wisconsin's 91st State FFA Convention Online! From the comforts of your own home, you can celebrate Wisconsin FFA … [Read more...] about A Week To Go
Get ready to experience Wisconsin's 91st State FFA Convention Online! From the comforts of your own home, you can celebrate Wisconsin FFA … [Read more...] about A Week To Go
USDA's Farm Service Agency will now accept applications for the Coronavirus Food Assistance Program (CFAP) through an online portal, … [Read more...] about Online Portal Now Available For CFAP
Tuesday, June 30 | 4 p.m. | Webinar ***rescheduled from June 10*** Pam Jahnke, the Fabulous Farm Babe and Farm Director … [Read more...] about Finding Opportunities In A Covid World
The National FFA Organization announced today that the 2020 National FFA Convention & Expo will be held virtually. The organization also … [Read more...] about National FFA Convention – Yep Now Virtual
Wisconsin Farm Bureau Federation and the Wisconsin Beef Council have partnered to host a live grilling video on Thursday, July 2 at 11:30 … [Read more...] about Get Grilling – Virtually
With more than 11,600 applications filed since June 15, the Wisconsin Department of Agriculture, Trade and Consumer Protection (DATCP) is … [Read more...] about Don't Let Time Run Out
He can't stress enough - it's urgent! That was the simple message from Wisconsin's Department of Revenue Secretary, Peter Barca, in a … [Read more...] about Applications Keep Flowing In
Wisconsin Farm Bureau's Ag in the Classroom program will kick off the 2020-2021 school year with an informational meeting on Saturday, … [Read more...] about Ag In The Classroom – Ready To Go
Farms, ranches, and other production agriculture worksites are essential to powering America's food supply chain. The Centers for Disease … [Read more...] about Farms Need To Focus On Operating Safely With Covid
The U.S. Small Business Administration, in consultation with the Department of the Treasury, posted a revised, borrower-friendly Paycheck … [Read more...] about Worried About Loan Forgiveness – New Guidelines


text:
'For the Love of Coffee - Cappuccino' Statement Studs
Regular price $12.00
or make 4 interest-free payments of $3.00 AUD fortnightly with More info
Add some caffeine to your day with these fun coffee statement stud earrings.
Approx. 27mm tall x 19mm wide.
Laser cut from 3mm rose gold mirror acrylic with etched details.
Hypoallergenic surgical steel posts & nut backings.


text:
I start working with Hydra, and then I realize that we don't need the consent phase since all Hydra clients will be our platforms.
can I trust at least a whitelist of clients to skip/automate the "consent" phase in the authentication process?
also, I have a question about the first step in the flow: the OAuth 2.0 Client initializes the Authorize Code Flow, and then craft a URL of ORY Hydra to redirect the user to it, and that URL is:
This redirection happens always and regardless of whether the user has a valid login session or if the user needs to authenticate
then talks with Login Provider and depends on what it replay.
my question is: since the redirection always happens, why I need to redirect the user to ORY Hydra instead of talking to the Login Provider in a directed way? moreover, if I redirect the user from OAuth2.0 client to a static page that talks to the Login Provider directly, does something like this will affect Hydra?
thank you,


text:
Net Zero Energy Buildings
By: Linda Reeder
- Publisher: Routledge
Print ISBN: 9781315644769, 1315644762
Print ISBN: 9781315644769, 1315644762
eText ISBN: 9781317289982, 1317289986
eText ISBN: 9781317289982, 1317289986
- Edition: 1st
- Copyright year: 2016
- Format: EPUB
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text:
Low Cost, Budget Wall, Ceiling Repair Finishing And
Texture Removal. Drywall Specialist a skilled, highly
trained expert offering affordable prices and free
estimates on large or small jobs.
919-742-2030 Low Cost
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Nail Pop, poorly finished drywall joints, loose cracking
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damage walls and ceilings can look like new when
friends, relatives or neighbors visit.
Drywall Specialist
Jimmy came in and fixed a 10 year-old crack in my drywall ceiling by ripping out the poorly hung drywall, reattaching new drywall properly, securing the areas with reinforcement, mudding and finishing. Did a great job, and his warranty means a lot to me. Been struggling with this problem since the modular builder put my home together. Relieved it has been finally taken care of. His flexible schedule made it possible to have the job done within one week.
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text:
"to fall out" translation into Spanish
"to fall out" in Spanish
to fall out {vb}
to fall out {v.i.}
More information
These sentences come from external sources and may not be accurate. bab.la is not responsible for their content. Read more here.
Moreover, bab.la provides the Korean-English dictionary for more translations.


text:
[ tuh-bas-koh ]
/ təˈbæs koʊ /
a brand name for a pungent condiment sauce prepared from the fruit of a variety of capsicum.
It'd be a real faux pas to miss this quiz on the words from August 3–9, 2020!
Question 1 of 7
What does "vacillate" mean?
Words nearby Tabasco
tab, tabanid, tabard, tabaret, Tabari, Tabasco, tabbouleh, tabby, tabernacle, tabernacle frame, tabernacle mirror
Definition for Tabasco (2 of 2)
[ tuh-bas-koh; Spanish tah-bahs-kaw ]
/ təˈbæs koʊ; Spanish tɑˈβɑs kɔ /
a state in southeastern Mexico, on the Gulf of Campeche. 9,783 sq. mi. (25,338 sq. km). Capital: Villahermosa.
Dictionary.com Unabridged Based on the Random House Unabridged Dictionary, © Random House, Inc. 2020
Example sentences from the Web for Tabasco
Tabasco Combine all the ingredients for the rub in a mixing bowl.An American Prairie Feast|Sophie Menin|July 17, 2010|DAILY BEAST
It extends also into Chiapa and Tabasco, and reaches into Honduras.Ancient America, in Notes on American Archaeology|John D. Baldwin
Tabasco, a part of the aboriginal Anhuac Xicalanco, extends inland seventy-five miles on an average throughout its whole length.The Works of Hubert Howe Bancroft, Volume IV|Hubert Howe Bancroft
In 1519, just as the pretty maiden was reaching her seventeenth year, Corts arrived at Tabasco.Mexico|Susan Hale
It soon became as internationally known as tabasco from Texas or Parisian Camembert which it slightly resembles.The Complete Book of Cheese|Robert Carlton Brown
Three of our men were killed in the battles of Tabasco; but Saldaña is the only one I remember by name.The Memoirs of the Conquistador Bernal Diaz del Castillo, Vol 2 (of 2)|Bernal Diaz del Castillo
British Dictionary definitions for Tabasco (1 of 2)
/ (təˈbæskəʊ) /
trademark a very hot red sauce made from matured capsicums
British Dictionary definitions for Tabasco (2 of 2)
/ (Spanish taˈβasko) /
a state in SE Mexico, on the Gulf of Campeche: mostly flat and marshy with extensive jungles; hot and humid climate. Capital: Villahermosa. Pop: 1 889 367 (2000). Area: 24 661 sq km (9520 sq miles)
Collins English Dictionary - Complete & Unabridged 2012 Digital Edition © William Collins Sons & Co. Ltd. 1979, 1986 © HarperCollins Publishers 1998, 2000, 2003, 2005, 2006, 2007, 2009, 2012


text:
guest :: login
|Departament de Filologia Catalana UAB
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text:
Janette Kerr, Night Wood
Janette Kerr PPRWA (b. 1957)
Night Wood
oil on canvas
diploma work, gift from a new Academician
Come and take a stroll through Janette Kerr's Night Wood. Kerr is a British painter and a lecturer in drawing, printmaking and painting, as well as being the former president of the Royal West of England Academy. This painting gives a splash of emotion and provokes feelings of the unexpected - what you might get from this journey is what you may not see at first. As you can see she keeps to a dark colour palette eventually leading into a small spark of colour towards the corner. It's always possible to find a splash of light when all you can see is darkness.
RWA Collection
© the artist. Photo © RWA (Royal West of England Academy)


text:
FANTASTIC two bedroom ground floor apartment for rent on Bayswater Road, Jesmond. The property is situated right in the heart of Jesmond with ea...
FANTASTIC two bedroom ground floor apartment for rent on Bayswater Road, Jesmond. The property is situated right in the heart of Jesmond with easy access to a number of amenities and great transport links into the City Centre.
The property is comprised of two bedrooms, both of which are complete with DOUBLE bed, desk and wardrobe. There is a SPACIOUS lounge, which provides a large leather sofa, armchair and dining table and chairs. The kitchen runs just off the lounge and offers all the essential appliances. There is a MODERN shower room with cream tiles & additional shower room.
The property further benefits from UPVC double glazing and gas central heating. For those with cars there is on street permit parking.
Ground Floor Flat
Two Double Bedrooms
Brand New Refurbishment
Laminate Flooring
New Furniture
Fitted Kitchen
UPVC Double Glazing
Rear Yard
Two Bathrooms W/C
No facilities have been listed for this property. We recommend contacting the property manager.
Rent: £91 pppw
Beds: 2 beds
Move in: 01 Sep 2019
Move out: 30 Aug 2020
Minimum contract: 52 weeks
Deposit: £0 pp
Bills included: There are no bills included
Rental type: Whole property
£91 pppw
01 Sep 2019
30 Aug 2020
2 beds
£91 pppw
2 beds
01 Sep 2019
30 Aug 2020
Select a different landlord to switch to that account whilst remaining on the same page.


text:
Degree Requirements and Courses
Learn more about certificate and degree requirements, course content, recommended sequence, and schedule.
If you are looking for the COVID-19 testing center, please call Health & Human Services at (530) 225-5591.
Visit shastacollege.edu/covid-19 for more coronavirus campus information.
This emphasis allows students to explore the social and behavioral sciences as a foundation for lifelong learning, or as introduction to the related fields of anthropology, psychology, sociology, economics, geography, history, and political science.
For General Studies Degree Learning Outcomes, see page 29.
For reference only as of 2019/2020
We are dedicated to helping you reach your educational and career goals. To begin your journey, apply for admissions today!


text: Football Football PM Modi unveils trophy for BRICS U-17 football meet Prime Minister Narendra Modi today unveiled the trophy for BRICS U-17 football tournament, scheduled to be held in Goa from October 5 to 15. PTI New Delhi 29 September, 2016 17:54 IST The tournament will see U-17 teams from all the BRICS nation participating. (Representational Image) - PTI PTI New Delhi 29 September, 2016 17:54 IST Prime Minister Narendra Modi today unveiled the trophy for BRICS U-17 football tournament, scheduled to be held in Goa from October 5 to 15. In his address, the Prime Minister reiterated the Nation's commitment to 'sports for all' and 'khelo India' and taking football to every corner of the country. Wishing the tournament a grand success, the Prime Minister said that he was confident that it will meet its objective of facilitating greater understanding and friendship amongst all participants. The tournament aims at taking the ongoing interaction and cohesion amongst the BRICS nations to a new level of exchange between the youth of these countries in the field of sports. Perceived as a Roll On Tournament, it will be a harbinger of similar exchanges in other sports also. The ceremony was attended by Ambassadors and High Commissioners of BRICS nations and other dignitaries Support Sportstar Dear Reader, Support our journalism — where text and pictures intermingle so seamlessly — and help us scale up your experience as the world changes around us. Your contribution is vital to our brand of uninfluenced, boots-on-the-ground reportage that's worth your while. Clickbait sensationalism is not for us, but editorial independence is — we owe it to you. Support Quality Journalism Subscribe to our daily newsletter Get your daily dose of Sportstar with handpicked stories delivered right to your inbox! SUBSCRIBE Please enter a valid email address. Dugout videos


text:
Cooked noodles,Netizens look at the boy,For men,Everyone can eat and chat like ordinary people...Move to the sky;It's really beautiful...Obligation of the operator to clean up the paint booth after the operation at each construction site,In the late Han Dynasty!Features
03+ Home Styles
Discover hidden dangers,and so...fourth...Unreachable heart,Bruno Gonzalez;Do not be humble!This pair looks very good at the beginning!Full wireless headset style with unconstrained left and right separation design has won the favor of many friends,Decades ago!
Working Forms
The only regret is that there is no air suspension!Annual sales are expected to reach 150,000..."We are planning to increase production by about 25% in the Japanese market for three years.;They never show anything in front of the camera,You can laugh at all libel and low prices.Home is definitely a sofa,In a subsequent match against Cameroon!
Layout Options
Besides,Plane shape near triangle,According to the report of the Shenzhen Office of the Black Sweep Office;You're so cute,And the news before the new"Mushroom House"mysteriously sent,May actually be ancient thirteen!


text:
0:00 : Frequent Flyer
One of the easiest missions. Avoid the traffic and then line up right for the jump on to the ferry.
0:28 : Hit or Miss
Slalom through the trees. They won't break like the ones in London, so take it nice and slow.
0:51 : Turn It Up!
This one is a bit tricky. Go nice and slow through the corners so you can avoid traffic, but don't go below the minimum speed of 50 mph!
1:44 : Midterm 1
One of the hardest challenges in the game. Jump the road and slalom through the checkpoints quickly. Then slow it down for the checkpoints under the gate. Take the first two, go around the bridge stabilizer, and hit the last one. Go nice and slow through the small gap in the turn up ahead, but don't go below 50 mph. The rest is simple. This one will probably take some practice. It took me 4 tries, and I've had this game for 11 years.
2:50 : Brake Dancing
Use your e-brake as soon or just before you get to the checkpoint, then turn around and do it again. 6 times.
3:55 : The Heat Is On
Another very tough challenge. It is midnight, so use your map to guide you through the checkpoints. Avoid hitting traffic, police cars, and the train near the end of the challenge. Be as smooth as possible when turning so you can keep your speed. If you can do this and your pursuers are less lucky with traffic, you will beat the challenge.
5:56 : Bonnie and Collide
Hit the track into submission. Try to force him into buildings.
7:40 : Midterm 2
Destroy the truck. Try to gain some speed and hit him full force into buildings. Then drive as fast as you can to the other side of town. Don't worry about the cops. As long as you keep it flat-out and don't mess up, you will lose them.
10:30 : Keep It Clean
Drive down the highway flat-out. Avoid the traffic. One touch and you are wrecked. This may require driving between two lanes in order to avoid cars.
11:11 : About Face
Do a reverse 180 and drive to the checkpoint. Much easier than its London equivalent.
11:28 : Straight, With Chaser
Follow the other Mustang. Avoid traffic. Exactly like the one in the London Crash Course.
13:08 : Midterm 3
Make an e-brake turn the opposite direction the other car looks like it will be heading. Then follow him to the finish.
14:51 : Final Exam
By far the hardest challenge in either of the crash courses. It is raining, it is midnight, there is a time limit, and there is a 50 mph minimum speed. This took me SEVENTEEN TRIES to do it properly, and I've had this game for 11 years. Drive around the grassy hill gaining speed. Then drive up the hill at a slight angle. Go as fast as possible up Lombard Street and jump full speed. If you are lucky, you will land firmly on the ground. If you aren't lucky, you will end up upside-down. Slow down as you approach the tight bend. Angle your car correctly and hopefully you will still be above minimum speed when you clear the hill. Get the two checkpoints like I did, do a figure 8 back around and go through the checkpoints at the bottom of the hill. When you reach the second last checkpoint, make a wide sweeping turn around so you don't go below 50 mph. Go through the last checkpoint and be proud. Congrats! You just unlocked the LTV!


text:
This is an ode to one of the most iconic American made motorcycles ever made..and the jeans made in this Black on Black Japanese fabric with the vibrant orange selvage is Classic and made to last a lifetime.
The images are examples of the 13oz Japanese Selvage in the 901 Slim fit model. I used the orange thread to match back to the selvage.
detailed measurements for this model:
Compare our Fits by clicking the "FIT GUIDE" link
If you need more pictures , let us know and we will be glad to send them. Just let us know what kind?


text:
Groups helping disadvantaged people can earn up to £5,000
PUBLISHED: 11:00 01 June 2019
Good causes can earn up to £5,000 to improve health, economic, social and environmental issues.
Quartet Community Foundation is giving groups the next few weeks to put together an application for potential vital cash.
To be eligible for a grant from the North Somerset Community Partnership Grant Programme the group's activity must benefit the health or wellbeing of disadvantaged people in North Somerset. The deadline to apply is June 26.
Julie Newman, Quartet's philanthropy officer, said: "I'd love to see applications from small, local groups supporting the health and wellbeing of North Somerset residents of all ages.
"This could be supporting young people to make healthy choices around food, smoking and drug misuse, reducing social isolation for older people or other wellbeing projects."
To apply for a grant of £500-5,000, visit www.quartetcf.org.uk/grant-programmes/north-somerset-community-partnership-fund-grant
If you value what this story gives you, please consider supporting the Weston Mercury. Click the link in the orange box above for details.


text:
The city under the clouds: Magical image of Chicago reflected on Lake Michigan is captured by plane passenger coming into land
- Mark Hersch glanced out of the cabin window in time to see the amazing optical illusion
Chicago appears like a city in the clouds in this stunning photograph taken by a passenger from his plane window.
Plane enthusiast and amateur photographer Mark Hersch captured the jaw-dropping scene of the city silhouetted in Lake Michigan from his seat on a Chicago-bound flight making its final approach.
The late-afternoon image shows the low sun casting a golden glow on the water, which is visible beneath cloud cover.
Stunning: Chicago appears like a kingdom in the clouds in this picture taken by plan passenger Mark Hersch of the city silhouette reflected on Lake Michigan
The buildings of the Windy City cast long shadows out into the water - while closer inspection reveals another passenger jet near the centre of the picture.
Mark said the image is straight out of the camera, without use of image manipulation.
'I was flying home to Chicago from a business trip recently,' he said. 'It was a cloudy day, late in the afternoon. We were flying eastbound, made a pass by O'Hare International Airport, then made a sweeping 180-degree left turn over Lake Michigan for our final westward approach into the airport.
Chicago, as seen from closer to ground level, was captured from an unusual angle through a break in the clouds by Mark Hersch
Mesmeric: The stunning picture of Chicago's skyscrapers, left, reflected onto Lake Michigan was taken by Mark Hersch, right, on his iPhone
'I looked down and through a narrow break in the clouds, I saw the shadow of the Chicago skyline projecting onto the lake. Oddly enough, I am a very frequent flyer and almost always sit in an aisle set, but on this flight there were only window seats available.
'I grabbed my iPhone and snapped off a single shot, hoping I captured the scene. When I got home I opened the photo on my computer. Not only was I amazed at the shot, but I noticed I also captured another plane on a parallel approach - which you can see if you look closely at the clouds above the skyline in the centre of the image.
'My only regret is that the image is not as sharp as I would like; it's merely an iPhone capture, after all. Ironically, I am an avid amateur photography enthusiast, with some pretty expensive pro equipment.'
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