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How can you not love a dandelion?
I ask this because a dandelion is one of the first bright signs of spring. Their yellow flowers spread along the ground like stepping stones of sunshine heralding the change of season all sun lovers welcome. I understand there are those among us who see the dandelion as a dreaded weed to be controlled as to not upset the balance of a green monochromatic landscape, but I would suggest a more wild and colorful reflection in one's yard is a representation of a life being lived fully.
Beyond visual appeal and seasonal anticipation, there are many reasons to welcome the dandelion into your life. A few of my favorite have to do with eating, of course! As my knowledge of food as medicine grows, I am fascinated by the healthy benefits of the many plants growing in our own yards and nearby forests.
Dandelions are completely edible from roots to leaves to flowers, and the many ways of enjoying these edibles is evident in the many recipes you can find. Dandelions have been on the human plate throughout history and if we know anything from history, it is everything serves a purpose. Our ancestors chose the plants to eat for a reason. They did not have the science we have today to determine a plant's properties, but they knew what made their bodies function well!
It may seem strange at first to eat a flower many of us have grown up thinking of as a weed. However, after reading what medicinal plant experts and enthusiasts have to say, you may welcome the dandelion as more than just a sign of spring, but instead as a sign of nature's bounty and gift for increased vitality.
Here are the basic nutritional facts. Dandelion greens are a tremendous plant based source of vitamin K. One serving of dandelion greens provides 535 percent of the recommended daily value of this important vitamin known for bone health and as a support to the circulatory system meaning heart health! Dandelion greens are a rich source of beta-carotene, which the body converts into Vitamin A. One serving of fresh dandelion greens provides 112 percent of the daily minimum requirement of vitamin A translating into medicine for healthy skin, eyes, and mucous membranes. These greens are also loaded with vitamins C and B5, calcium, iron, potassium, and manganese.
In one statistical comparison I reviewed, dandelion greens ranked ahead of both broccoli and spinach in overall nutritional value. Two other top ranking nutritional powerhouse weeds are amaranth (pigweed) and lambsquarters. Collards are the only domestic green to break into the top four followed by broccoli and spinach. As if this were not enough, dandelion is an excellent source of lecithin. Lecithin is a nutrient that elevates the brain's acetylcholine, a substance that helps maintain brain function and may play a role in slowing or even stopping the progression of Alzheimer's disease. As a bonus, lecithin also helps the body maintain good liver function.
Now that you have the nutritional scoop, here are a few easy ways to include dandelions in your diet. Dandelion greens are bitter when eaten raw. Bitter greens are good for digestion, but if you want to make them more palatable- combine them with other lighter greens or perhaps a honey dressing and some bits of dried or fresh fruit. Eaten raw, they will retain the most nutrition. If you cook the greens, keep it to a quick cook tossing them into eggs, pasta, or on a pizza.
|Dandelion greens in a quick sauté with fresh asparagus, shallots, garlic, and morel mushrooms. Delicious on a pizza !
|Spring Pizza
Dandelion Flowers can be eaten raw in salads, dried for tea, or used to make wine, vinegar, or jelly. The possibilities are only limitless if you stretch your taste preferences and culinary creativity!
If you are feeling industrious on your dandelion harvest, bring along a spade and dig up the whole plant. The nutritious roots contain a milky sap that contains valuable properties. Steam the whole roots to seal in the sap. This happens when the sealed roots don't release the sap when cut. Once steamed, cut the roots into pieces. Air dry on a plate covered lightly with a paper towel for a day. Spread the root pieces evenly on a baking sheet and roast in a 250 degree oven until completely dry. A lighter colored roast will impart a milder flavor and a darker roast will have a stronger aroma and flavor. The pieces can be stored in an airtight jar and used for making tea, or the pieces can be ground and used as a coffee substitute.
Before you head out to harvest your own wild edibles, here are a couple of things to keep in mind…
~remember to harvest only from an area that has not been sprayed with any chemicals and stay away from any areas frequently visited by your pets!
~if you have allergies to ragweed, marigold, mums, daisies, or yarrow, you might be allergic to dandelion flowers, too.
Be curious and have fun exploring what the earth abundantly provides!
The Dandelion Celebration by Peter Gail – a great book for tons of recipes and general information on history and benefits of eating dandelions.)
http://foodfacts.mercola.com/dandelion-greens.html– a fabulous website for everything about the body and keeping it healthy
http://www.vitaminstuff.com/herbs-dandelion.html– one more resource on the dandelion's health benefits


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Russia fails to create power grids operator in occupied Crimea – media
16:20, 11 December 2017Economy
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Russia's RosSeti power company, which comprises interregional and regional distribution grid operators, may fail to become a key shareholder of JSC Krymenergo, which is being created as a regional electricity operator in the Russian-occupied Crimea, because of the risk of falling under international sanctions applied to the peninsula, according to the Russian daily Kommersant, referring to its sources.
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Hello scrappy friends, time for my weekly Pocket Scraping update. For those playing along, we are now beginning WEEK 11!! Time really flies when you do a weekly scrapping layout! Here are week's 8 and 9 from my 6x8 Sn@p Life album:
And my 5 tips and tricks for the week:
- Are you celebrating St Patricks Day? Check out these free printables from Crystalwilkerson.com
- Looking for inspiration from a fellow Project Lifer? Stop by and see Seriously Sabrina!
- I am convinced that by using all of my scraps in combination with the amazing freebies out there, I can make this year of pocketing pretty darn cheap. Melanie Britt is another great designer offering up a TON of freebies!
- My 12-year old introduced me to a fun app called Color Splash. It makes your photo black and white, and then you color in any parts of it with any colors you'd like. Great way to highlight something on a not-so-great pic.Here's an example:
- Want to try and print a jumbo pic and then cut into segments to fit in your pockets? Check out the tutorial over at Sweet Rose Studio to get this look:
Thanks for stopping by, have a great week! =) T


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Our Values
Enhance initiator Share resources Diverse learning and joint achievement
Enhance the value format of the initiator,
starting from self, and of belonging to the social environment and its institutions as a citizen with rights and duties, through marketing and practice of citizenship concepts, community leadership and peace-building with an emphasis on faith in the ability of every initiator to innovate and invest all options to interact with everyone and improve the quality of life, upping the initiative in work, empowerment of achievement and commitment to those values and concepts.
Share resources and exchanging knowledge
the sharing of resources and the exchange of knowledge among the members can provide community-based initiatives that help meet the needs of their local communities in more professional ways, building upon the impact of initiatives, each upon another which enhances the sustainability and builds positive awareness resulting from the practice and reflecting them on a national level.
we are working to respect the differences that exist in our societies, and the monitoring of richness found in our different local identities, races and religions to formulate such cultural diversity of the country.
Diverse learning and joint achievement
respecting the freedoms of others is one of the most important success factors of Mobaderoon, by respecting the positive values of our communities and public morality, far from any political orientation, religious or ethnic, thus contributing to the building of a safe common interaction platform based on trust and common understanding of their identities, which everyone knows and interacts through their experiences, knowledge, learning and the joint achievement of local challenges, while ensuring ownership of their ideas and their interventions in communities and reverse the impact at a national level.
Part of all
Mobaderoon relies on cooperation with all organizations working in the field of development and participatory process with activists and community development work, personally and institutionally.


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I love the pattern so much that I've decided that for the up coming long weekend I will dedicate my time to knitting at least one pair of them. I have several other projects I want to attempt to complete also. But my weekend focus is definately going to be Fetchings.
4 years ago


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Jason Ritter's in the "Bag" on Facebook Live
Posted by Matt Singer on
When I sat down next to Jason Ritter, his first words to me, even before I could introduce myself, were "What was it like interviewing R. Kelly for 'Trapped in the Closet?'" That's why I was excited when I got to host Ritter's solo chat on Facebook Live after our first interview with the whole crew from his SXSW selection "A Bag of Hammers." You figure a guy digs "Trapped in the Closet," he's probably a fun dude to talk to. And he was. After talking a little more about "Bag of Hammers," the star of NBC's "The Event" and I got down to the really important stuff: like what it was like shooting a cameo for Nicolas Cage's "The Wicker Man" and being witness to the greatest fight in movie history, "Freddy Vs. Jason." It was a great interview, almost as cool as chatting with R. Kelly at the Chocolate Factory.


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Monica and Walter Noel hailed from Greenwich, CT. They had purchased a giant home called Discovery. It looked like a set from a James Bond movie á la the reign of Roger Moore. The bedroom had open construction walls and a pool in it that connected directly into the living room, providing a convenient view. The Noels knocked it down and constructed a palapa in its place, called Yemenjá. It expressed Monica's Brazilian heritage. The name Yemenjá originates from the Brazilian Goddess of the ocean and motherhood. This home was meant to be their big, showpiece vacation place and it was a giant property even for Mustique.
I had met Monica at Linda de Pecciotto's opening night party at Ocean Breeze. The party turned out to be not only a fabulously fun and exciting night for both Linda and I, but the best possible advertisement for my work that I could ever have hoped for. There were certain pieces that I still hear about from people, which they found unforgettable upon seeing them that night. In particular people were stunned by the pair of mirrors I commissioned for the dining room, they were by an artist in Paris who crafted them out of black muscle shells dipped in pewter, which were a tasteful interpretation on traditional shell mirrors. They were really stunning and people couldn't quite get over them.
One of the guests whose interest was piqued by my design flourishes was Monica. She immediately inquired through Linda about working with me. Linda, who was always generous when it came to sharing resources and was incredibly supportive of my career, put us in touch. At the time I was still working with Peter Marino and that turned out to be not the best fit for Monica and Walter. However, when I was ready to go out on my own several years later, Monica was the first person I talked to and she immediately brought me on to complete Yemenjá.
She had made a rather heroic attempt to design it herself, but a 50,000 square foot resort home that sleeps eighteen is a challenge even for a seasoned pro. As this was my first big project on my own, I was glad that it wasn't also my first job on Mustique as I might have lost my mind. Designing on Mustique is akin to designing on another planet so at least I had some of the basics down, but Yemenjá would bring its own unique challenges to the table.
The house is not only immense but it's on a steep incline. They hired an architect called Manolo Mestre who I had known for years. The moment Monica 'introduced' us was pretty hilarious as we immediately ran to each other and gave each other a giant hug. It seemed like incredible serendipity that they would have hired him.
Because she had been decorating the house by herself, Monica had already made a number of purchases. Monica has excellent taste and had chosen some great pieces that we were able to work into my specific vision for the villa. I became known as the "furniture butcher" for my reinventions of the existing pieces. This has since become one of my very favorite things to do. See this post for some tips on how you can do a little "furniture execution" of your own!


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Florence Welch's Eye-Catching Indie Style (PHOTOS, POLL)
British singer Florence Welch of the indie rock band Florence and the Machine is certainly one to keep an eye on. Her hit song "Dog Days are Over" catapulted her to stardom, as it earned her a VMA and Grammy nod, and she even performed at the Nobel Peace Prize Concert in Oslo last December.
On stage, Florence opts for Stevie Nicks-inspired outfits with dramatic sleeves to highlight her energetic arm motions and commanding stage presence. On the red carpet, the singer dons vintage-inspired ensembles with lace, velvet, and floral prints. She's not afraid to wear socks with heels or to highlight her flaming red hair or to turn up pantsless at a Chanel show, all of which we absolutely love.
Take a look at some of Florence's best looks and let us know which ones are truly marvelous.
Read More…
More on Celebrity Style


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Totti Affirms There Will Be inhibitors to Rome
Rome – Roma Star Francesco Totti gives his attitude about the talks related to a contract extension. He insists not want to be inhibitors for the Giallorossi and will accept any decision.
Totti contract with Roma will expire in June next year. Touted club would not grant an extension to the captain, who of course could force players to retire 38 years from the gridiron.
Problem is, Totti admitted to not overly complicate. He reiterated his support for the team, either as a player or as a fan.
"I want to clarify something. I never asked for a change to my contract. The owners of the club, the club, and I have always walked in harmony about it," Totti wrote on his Facebook page, as quoted by Football Italia.
"It is the passion that binds me with this uniform color. I have never and will never be a problem for Rome."
"I would be in favor of the pitch, the bench, and from the stands to the players who wear and will wear this uniform. I always desire and dream of the best for this team."
"Let the assessment done by the media and wait for the decision about the future until June. I can say that personal course play makes me happy, but at this point I evaluate a variety of ways beyond my presence on the field. I feel able to contribute both on the pitch or outside, "he added.
This season Totti has appeared 32 times in various competitive arena, scoring eight goals and contributed six assists.


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Get fit, lose weight and build muscle with one of our Women's Workout plans. Whether you're a fitness enthusiast or looking to start your fitness journey we've got a plan for you. Just beginning? Why not try our 30 day challenges! Want to be a bikini pro? Check out our ambassador Nina Ioanna's bikini body challenge.
What are you waiting for?
Yesterday you said you'd start tomorrow.


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Exercises to Ensure You Have a Hot Body for Prom
You obviously want to look your best on prom night.
After all the end of year eating and relaxing you could probably do with losing a few pounds before Prom.
With adequate preparation and the right methods it is entirely possible to shed that extra weight and tone those muscles before the big day.
The sooner you start the better.
Here are a few things you can begin to do right now to get that toned body come prom night.
Let's look at them:
Take up Pilates
Pilates is one of the most effective ways to lose weight.
It is also one of those workouts that can reduce the chance of injury thereby making it the perfect one to choose if you are new to fitness training.
Unlike other workouts that require you to learn a lot of different moves, Pilates focuses on few exercises but precise repetition.
There is also no need to worry about equipment as Pilates primarily uses your own body weight as resistance.
Weight Training for Strength Conditioning
Pilates will help you burn calories and hence lose a few pounds but strength training will help you build muscle and tone your body.
For this you may need some equipment such as dumbbells although you could also use your own body weight as resistance.
In order to do this correctly, it is important to get some help.
You can sign up at your local gym or if you can afford it get a personal trainer.
Yoga is great for strength conditioning.
This is because Yoga generally uses your own body weight as resistance.
You can do a little research to find Yoga poses for beginners or join a Yoga class in your area.
Get Rid of Cellulite
Most girls suffer from the debilitating effects of cellulite and saying that cellulite is unattractive is an understatement.
If this is the primary reason why you want to lose a few pounds before prom, you need to find exercises that are primarily geared towards getting rid of cellulite.
Regardless of whether the cellulite is on your belly, legs, arms or thighs daily cardio exercises combined with the right strength training can help with eliminating cellulite.
It is however important to note that a change in diet is a requirement if you are going to diminish cellulite.
It is even more important that you avoid advertisements that promise a quick fix for your cellulite problems.
Most of them don't work and even have a number of side-effects you may not be ready to deal with.
Include Aerobic exercises
One of the best ways to burn extra calories is to have some kind of aerobic exercise.
You can join your local gym and sign up for an aerobics class or a Zumba class.
If that is not an option for you simply jumping rope will work.
It is a relatively inexpensive way to get your aerobic exercise not to mention that it can be quite fun.
Start with a basic jump and then increase the intensity as you go along.
To make it interesting, you can ask your friends to do it with you.
You can even designate a particular time of day to do it like say in the morning before school or evenings.
As much as all the ideas described above are great ways to lose weight and get a toned body, none of them will work unless paired with healthy eating habits.
For instance, it is very important that you have a healthy breakfast before the start of each day.
Skipping meals most especially skipping breakfast will slow down your metabolism as your body works to conserve energy.
This will in the end hamper your efforts to lose any weight.
It is therefore important to ensure that your daily nutritional requirements are met on a daily basis so that you lose weight safely and remain healthy.
Including carbohydrates such as whole grains and cereal in your breakfast as well as healthy fats and proteins will help you stay healthy and give you the energy you need to workout.
Don't forget to read our article about eating healthily as well.
Featured Image Credit


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Wilderness Medicine Protocol Package 4th Edition (Digital Download)
$ 149.99
The Wilderness Medicine Protocol Package will set your field program up with the most current curriculum and easy-to-follow protocols for backcountry treatment and evacuation, saving you weeks of work. Each protocol offers you decision points to help customize them for your organization and staff, so you can keep doing what you're doing outside. Also included are resources to help you work with a medical advisor, updated medical-legal information, and tips for what to carry in a first aid kit.
Digital contents:
- Wilderness Medicine Overview
- The Role of a Medical Advisor
- Medical Advisor Job Description
- Protocol Use Statement
- WFA Scope of Practice
- WFR Scope of Practice
- Abdominal
- Allergic Reactions and Anaphylaxis
- Altitude Illnesses
- Burns
- Cardiac Emergencies
- Cardiopulmonary Resuscitation
- Chest Trauma
- Dental Problems
- Diabetes
- Dislocations
- Eyes Ears Nose
- Flulike Illness
- Head Injuries
- Heat Illnesses
- Hypothermia
- Lightning
- Local Cold
- Mental Health and Stress
- Musculoskeletal Injuries
- Neurological Emergencies
- Poisoning
- Respiratory Emergencies
- Shock
- Skin
- Snakes and Lizards
- Spiders and Scorpions
- Spinal Injuries
- Submersion Incidents
- Urinary and Reproductive
- Wilderness Wound Management
- Zoonoses
- Drug Kit Considerations
- First Aid Kit Contents
- SOAP Report Template
- Verbal SOAP Template


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Meet the Hosts of the 2015 Independent Spirit Awards
Posted by Melissa Locker on
It's the 30th anniversary of the Independent Spirit Awards and it takes a big celebration to properly mark the occasion. That's why Film Independent President Josh Welsh announced today that Portlandia's Fred Armisen and Kristen Bell will co-host the 2015 Film Independent Spirit Awards.
"The only thing better than having a great host for the Spirit Awards is having two great hosts," said Welsh. "For our 30th anniversary we're so excited to have Fred and Kristen co-hosting the awards and it's going to be a fantastic show."
Jennifer Caserta, president, IFC, commented, "We're proud to once again showcase the Independent Spirit Awards on IFC and celebrate the 30th anniversary with a live broadcast. Fred is obviously right at home on IFC and we are excited to welcome Kristen to the network. Together, they'll make this show true event television for our viewers."
The ceremony will once again take place during a daytime luncheon in a tent on the beach in Santa Monica on Saturday, February 21st with the premiere broadcast airing live exclusively on IFC at 2 pm PT / 5 pm ET.
Want the latest news from IFC? Like us on Facebook and follow us on Twitter@IFC


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We at LifeQuest are seeking God's blessing in the chaos. Read more here.
Event Updates: The LifeQuest Freedom unBanquet is underway! Learn more here.
The 7th Annual Charity Golf Classic will be Oct 12, 2020, Raffle Tickets on sale now, and registration is open!
LifeQuest is a non-profit serving the adjudicated youth throughout New Mexico. We seek to offer at-risk and incarcerated youth in the state of New Mexico a pathway to a brighter future and hope for life fulfilled. We offer many group programs and one to one mentoring.
LifeQuest has been serving in our communities for well over a decade and is excited to be the NM Charter Affiliate for Ambassadors of Compassion. AOC is a coach lead youth leadership program that is non-religious and non-political. AOC originated in Orange County CA and has spread through the states to build resilient youth across the nation. The Program Launch is set for 2019/2020 school year.


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In Homerism's experience growing up, even the best students had at least one subject they just couldn't master. While they typically breezed through most of their classes, there was always that one that gave them a surprisingly tough time. (Think Anthony Michael Hall and his shop class woes in The Breakfast Club.)
Archive for February, 2009
Study Hard, 'HornsFebruary 28, 2009
On the Spot: Mike LeachFebruary 27, 2009
2008 was supposed to be the year Texas Tech made the leap. Contrarian offensive guru Mike Leach had all the pieces in place for the Air Raid attack to flourish. The move in midseason 2007 to put Ruffin McNeil in charge seemingly transformed Tech's defense overnight. Coming off a strong finish the year before, numerous preseason prognostications put the Red Raiders in the top 10.
- Tech pasted a tissue-soft out-of-conference schedule of Eastern Washington, Nevada, SMU and Massachusetts. Nothing new about that.
- The Raiders pulled off a big win in Lubbock over a quality opponent–Texas, in this case. Seen that one before.
- Leach's O rolled up some gaudy numbers. Old hat at this point.
- Tech's allegedly improved D gave up points in bunches. Very familiar.
- Despite a supposedly newfound toughness, the Raiders were outmuscled–badly–by more physical opponents Oklahoma and Ole Miss. Same old story.
Bustin' Out: Jacory HarrisFebruary 26, 2009
If you want to trace the steady downfall of the Miami Hurricanes, just take a look at the parade of quarterbacks who have been put in charge since Ken Dorsey graduated in 2003 with a national championship ring and another appearance in the title game.
The U's QB woes came to a head last season with prized recruit Robert Marve's ugly departure from the program. Head coach Randy Shannon yo-yoed between Marve and freshman Jacory Harris all season, creating an uncomfortable power-sharing arrangement that ultimately proved untenable. When Harris took a greater role in Miami's offense late in the year, it didn't exactly produce sparkling results, as the 'Canes dropped their final three games of the season. That included double-digit losses to Georgia Tech and North Carolina State.
In the Emerald Bowl versus California, Harris had a pedestrian stat line: 25-of-41 for 194 yards with two touchdowns and an interception. However, the young signal caller's numbers don't tell the whole story when it comes to his impact in that game.
Unfettered CapitalismFebruary 24, 2009
A New York Times article published today revealed that USC head coach Pete Carroll raked in a whopping $4.4 million in 2007.
Mack on a MissionFebruary 22, 2009
When Mack Brown sets his mind on something, he follows through.
"Since this situation has never happened before in the Big 12, I think the conference should follow the lead of all of the other BCS leagues with championship games in how they settle three-way ties. I think their systems are fairer and give more credit to how the two highest ranked teams performed against each other on the field," Brown said at the end of the regular season.
Combine TimeFebruary 20, 2009
I don't know what it is about the buildup to the NFL draft, but Homerism can't get enough of it. The difficulties projecting a college player's success in the NFL are fascinating. Plus, as what I consider to be a hardcore college football fan, I love it when I can pick out a college player who I know is destined for greatness or bust-dom on the pro level.
Who's Minding the Offense?February 20, 2009
The rube in Homerism wants to believe that USC coach Pete Carroll's performance at Mark Sanchez's farewell press conference showed his genuine concern for one of his players. My cynical side says it was motivated more by his genuine concern for the state the Trojans' quarterback situation.
Can the Trojans continue to plug in quarterback after quarterback and not miss a beat? With last year's starter Mark Sanchez making his debatable move to the NFL, USC will have a full-blown quarterback competition going this spring. The list of candidates is long and distinguished. Redshirt freshman Aaron Corp appears to be the frontrunner and is considered the most athletic QB prospect Carroll has had. Super-transfer Mitch Mustain from Arkansas has the billing, but he has yet to really take the reins. Incoming freshman Matt Barkley also is generating loads of buzz, even though it's tough to see a true freshman playing quarterback in Troy.
Year one of the RichRod era for Big Blue failed to identify a Pat White-type quarterback with the tools to trigger the spread option. The news that the relatively underwhelming Steven Threet was heading out for greener pastures has heightened the uncertainty in Ann Arbor. Consequently, year two could find a freshman under center. "Underwhelming" would be a compliment when discussing Nick Sheridan's play in 2008, but as of now, he's the only quarterback with any experience in coach Rich Rodriguez's version of the spread. That probably means he'll get most of the spring snaps. Early enrollee Tate Forcier will learn at Sheridan's side. However, both may be keeping a seat warm for when promising recruit Denard Robinson arrives in the fall.
Sailing On?February 18, 2009
Could college football's favorite pirate enthusiast really be walking the plank in Lubbock?
Do You Care About The Juice?February 10, 2009
I mean A-Rod and Mark McGwire and Barry Bonds. I mean Chris Benoit and Hulk Hogan and the Ultimate Warrior. I mean Mike Webster and Lyle Alzado.
As sports fans, I'd hope we're all way past the point of giving athletes–on any level–the benefit of the doubt anymore when it comes to the use of performance-enhancing drugs. I mean, here we had arguably the greatest baseball player of my generation up on ESPN this afternoon confirming a news report–as well as our long-held suspicions–that he was all 'roided up. Yet, to Homerism, the most striking part of the interview was that I was more interested in seeing how A-Rod handled Peter Gammons' questions than I was stupefied by his juice-y admissions.
But all of this goes college football, too. We hear stories about former Ohio State Buckeye Alex Boone hulking out and ex-Oklahoma Sooner Dusty Dvoracek beating the tar out of his supposed friend. Are we really supposed to believe these behemoths are just drunk, or maybe having a bad day?
Signed, Sealed, DeliveredFebruary 6, 2009
While some college football fiends like to portray National Signing Day as Christmas in February, that's not entirely true.
"I have to be real honest with you, recruiting has its own view," Miles said. "And I'm not going to dispel or concern myself with how that all fits."
OK, Homerism isn't even going to bother trying to parse that out.


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Double issue price: 8 EUR
Delivery costs are not included.
Slovene-English edition.
Ramsay Burt: Dnace, history, adn political relevance
Jens Giersdorf: »Hey, I won't let you destroy my history«: East German dance theatre and the politics of restaging
Bojana Kunst: Politics of avvestion and uneasiness
Krassimira Kruschova: Dance interrupts the visible. From the lecture series ob?scene at the Tanzquartier Wien.
Bojana Cvajić: Good objects exchanged for monstrums events. Speaking of the vicissitudes of the dance medium.
Rok Vevar: Forma Interrogativa
Nirvana Marinho: Sampling the media trough the body – a critique of the product of dance
Johannes Birringer: Networked environments for interactive dance
Petra Sabish: Scores of Processes. About Agency in Contemporary Dance/Performance REVIEWS
Mojca Puncer: Metamorphoses and/or homo-animalic promiscuities
Barbara Orel: Symbolism, simulation and the blind


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Cellular Protection Sun Cream SPF 50
How to Use
Smooth liberally over face and body before going out in the sun and re-apply after swimming or sweating.
About the Brand
In 2002, pharmacist Margo Marrone and her husband Francesco created a beautiful, wildly successful chain of wellness dispensaries in London devoted to their ever-expanding, luxurious, certified-organic line of homeopathic remedies, skin, hair, and body care. From a beautifully made dry brush to a seriously amazing detox kit and some of our favorite oils, scrubs, and soaks, this brand is a gorgeous, sleekly modern classic.


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Continuing Education
The goal of GC Real Estate School is to ensure you not only receive your required continuing education and post-license education hours, but increase the knowledge you need to improve your business.
Continuing Education and Post-License Education:
- Applies to all Kentucky Real Estate Licensees
- Required to attend six hours of continuing education annually
- Three of the six hours must be law
- Kentucky Core Course must be completed once every four years
- Deadline for CE is December 31st
- Check KREC website at krec.ky.gov to verify CE requirements & Core year
- CE requirement begins day PLE requirement ends
- Escrowed Licensees are NOT required to get CE
- CE Delinquency = $500 Fine!
What are my Continuing Education Requirements as a Kentucky Licensee?
Click on this KREC link to see what you have to do to keep your Kentucky License up to date: http://krec.ky.gov/Education/Pages/default.aspx
What is this Ethics requirement I keep hearing about?
The National Association of Realtors® has mandated that ALL Realtors® are required to complete Ethics training once during every 2 years cycle. **This includes any Licensee who is "grandfathered" through the KREC. For more info on this requirement, please go to https://www.nar.realtor/code-of-ethics/training
Why don't all of the classes I have taken show up in my Education History?
To get information about your full history of classes taken, please call the KREC at (502) 429-7250 or visit http://www.krec.ky.gov/ If you have taken a class that does not appear on the KREC website, forward a copy of the class certificate to provide proof of completing the class.
What if I have a special circumstance?
Sometimes people have special circumstances whether it is that they missed 3 hours last year, or they have medical issues, or they were in escrow but came out, etc.
We will help you to the best of our ability, but if you have special circumstances we recommend that you call the Kentucky Real Estate Commission (KREC). They are the final authority when it comes to your license and the requirements to keep it.
What you need to know before coming to class:
The Kentucky Real Estate Commission (KREC) requires the following in order to receive CE credit:
- Your signature on the sign-in sheet. If you are taking a full-day class, you will also need to sign-in when returning from lunch.
- A completed evaluation, which includes both your legal name and Kentucky Real Estate License number. If you do not know your license number, you may find it by searching the KREC database at http://www.krec.ky.gov/.
CE/PLE Classroom Regulations of the Kentucky Real Estate Commission:
- No one may enter a class 10 minutes after the assigned start time. The same rule applies for returning from breaks and lunch. No one will be re-admitted into the classroom if returning late from break or lunch.
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- Students will receive a ten-minute break every hour. These are the allotted times for using the facilities and taking calls.
- Students are expected to give their full attention during class. Please refrain from holding side conversations, reading materials other than the course hand-outs or taking unscheduled breaks.
- No credit or refund will be given to students barred for violation of these regulations.


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- This event has passed.
August 27, 2018 @ 10:30 am - 11:30 am
An event every week that begins at 10:30am on Monday, repeating until December 17, 2018
An event every week that begins at 10:30am on Monday, repeating until May 24, 2019
This postpartum class is a perfect place to restart or initiate a fitness regimen after baby has arrived. Bring your baby and get a sweat on while being led through a safe and effective exercise program designed to address the areas of your body that have been challenged by your pregnancy and delivery. Mamas may join this class after their 6-week postpartum check up and continue until baby becomes mobile.
Location – Sadhana Yoga in Wilmington, DE
Cost – $70 for 5 classes
Please check the the Sadhana Yoga Website to confirm class time.
Please Pre-Register for Classes
Please e-mail us with questions or concerns at firstname.lastname@example.com.


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By GREGORY ZELLER //
A key e-commerce alliance has pushed an innovative skin-care company past $1 million in revenues – and has the biotech's only full-time employee thinking expansion, in more ways than one.
Kantian Skincare, a 2012 startup and the public face of Huntington-based Kantian Sciences Corp., hurtled past the $1 million plateau in July, according to cofounder and President Jon Klein, on the back of the Neutralyze Anti-Acne Solution, the company's flagship product.
Sales of the multi-patented product hit seven digits just 24 months after Neutralyze hit virtual shelves, thanks primarily to Kantian's partnership with online-shopping kingpin Amazon.
According to Klein, more than 30,000 units of Neutralyze, which debuted on Amazon Prime in July 2015, had been sold by this summer. And while Kantian Skincare has recently begun accepting orders through its own online store, 95 percent of its sales have come through the international e-commerce titan – and quickly.
"It was quicker than we expected," said Klein, who launched the startup with his father, Smithtown dentist Richard Klein. "The largest factor would be our partnership with Amazon and having the products on Amazon Prime."
Not only does the mother of all e-commerce sites offer fantastic exposure, he noted, but being listed on Amazon – particularly Amazon Prime – carries an unspoken endorsement that significantly boosts a product's image.
"We've been able to really leverage the credibility that Amazon brings to your brand," Klein said. "In the customer's eyes, Amazon Prime has a higher level of credibility, because they know Amazon stands behind it and is offering customer support and order fulfillment.
"And most Prime customers already have their payment information stored in the system, because they feel confident knowing their personal information is secure with Amazon," he added. "That's why it's one of the biggest e-commerce websites in the world, and why it's such an advantage to be part of it."
Such advantages cannot be overstated when you're a fledgling biotech with one full-time employee and a virtual world to conquer. With production of Kantian Skincare's proprietary medications still contracted to a Pennsylvania laboratory and distribution handled largely by Amazon – with a nod to an Ohio-based fulfillment center that preps and packages the product – Klein oversees a burgeoning business with virtually no overhead, just freelance contributors and gallons of innovation.
The novelty largely involves Neutralyze's target audience. Featuring a "Nitrogen Boost" technology that combines keratolytic and antimicrobial activities in various acidic compounds, the product is geared specifically toward moderate to severe acne cases, a rarity among treatments that either claim effectiveness for all levels of acne or boast "maximum strength" for worse cases, but don't deliver.
By focusing on the moderate-to-severe market, Neutralyze has carved a rich niche, with customers inquiring often about new products in the line, according to Klein.
"A lot of our customers are asking when we're coming out with a moisturizer, when we're coming out with an anti-aging-acne hybrid," he said. "So, we're really focusing now on expanding the product line."
That effort involves consulting chemist George Deckner, a former Procter & Gamble research fellow wielding hundreds of patents and a fantastic reputation. Working with scientists at that Pennsylvania R&D facility, Deckner is the driving force behind new Neutralyze products slated to debut in January, including a "renewal complex" moisturizer for acne-prone skin and Neutralyze exfoliating pads, similar to classic Stridex pads but packing that patented "Nitrogen Boost."
"We've achieved a million dollars in sales with really three products in a singular acne kit," Klein noted. "The next logical thing for us, based on customer feedback, is to come out with these new products."
When they debut, customers will find the new products not only on Amazon Prime, but on the new Neutralyze.com website – Klein credits the site with about 5 percent of company sales to date, and rising – and on other Amazon channels.
Neutralyze has recently qualified for the Amazon Exclusives program for up-and-coming brands and also partakes of various Amazon pay-per-click services, including Amazon Headline Search.
The ultimate idea is to grow beyond Amazon, according to Klein, who described the e-commerce mothership as "a fantastic place for brands to launch" but not an endgame solution for a company like Kantian Skincare.
"Part of the reason we're trying to make this shift is so we can better own our customer data," Klein noted. "Amazon keeps all of its customer data. In terms of forming relationships with customers and being able to market to them directly by email, for instance, Amazon doesn't share that data.
"A lot of Amazon sellers, when they reach a certain plateau, want to diversity into their own e-commerce channels," he added. "That way, they can better communicate with their customers."
With targeted Facebook and Google advertising driving traffic toward the startup's online store and its Amazon channels, Klein believes that $1 million milestone is just the beginning – and he's envisioning growth for his five-year-old biotech beyond just its product line.
"Obviously, we want to grow and have a bigger team," he said. "We've been able to accomplish everything with just one employee and some freelance contributions, but growth is definitely the goal.
"We have some part-time hires now," Klein added. "I think over the next four months, we'll see the team growing for sure."


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After leading the way in the human exploration of space for nearly 50 years, the future of U.S. manned space flight is in question. The space shuttle makes its last flight next year. After that, NASA must rely on the Russians to put astronauts in space.
Unless the country looks to the private sector.
It may have to. A preliminary report from the Review of U.S. Human Space Flight Plans Committee (it even has a Facebook page) says current budget restraints are jeopardizing all future manned space flights even as NASA develops the Orion crew exploration vehicle that will replace the shuttle. (Check out Wired.com's post on the committee's report on Wired Science.)
"Really, we've given the White House a dilemma," Norm Augustine, the former Lockheed Martin CEO leading the panel, told PBS last week. "The space program we have today, the human space flight program, really isn't executable with the money we have. So, either we have to do something with the current program that's not going to be very successful, I'm afraid, or spend a nontrivial sum more than that to have something that's really exciting and workable, and that's the challenge the White House is going to have, is to sort that out."
The Orion resembles the capsules of the Apollo era and would be launched atop an Ares I rocket. The program was intended to support the International Space Station and lunar missions. The Ares I and Orion won't be ready until 2015 at the earliest, but some put the date several years beyond that.
So with manned space flight going on hiatus next year and some saying NASA needs a big infusion of cash to continue manned space flight, another option is emerging: NASA could use commercial ventures like SpaceX to deliver cargo and people to the space station.
NASA is bullish on commercial space ventures. NASA Administrator Charles Bolden and his deputy, Lori Garver, support such ventures, and the tight budget has them seeking new ways to meet the agency's goals, Jim Muncy, president of the PoliSpace consulting firm and co-founder of the Space Frontier Foundation, told Space.com in an excellent story on the issue.
But NASA contractor and aerospace giant Lockheed Martin says there's too much risk associated with commercial space flight to make that a viable alternative to a government program.
Aviation Week reports that Lockheed Martin believes the commercial space programs could cost a lot more – in terms of time, money and safety – than a NASA program. "We know how difficult it is to transport to the station and we don't want people to cut corners, and downstream having NASA pay the penalty of the time and cost of doing this," John Stevens, of Lockheed Martin's human spaceflight division, told Aviation Week.
That issue aside, Stevens wonders how the government is supposed to finance NASA and a contract with someone like SpaceX. "If we can't afford one program, how can we afford two?" he asks.
Stevens is obviously referring to Elon Musk and his SpaceX venture. It is one of several firms trying to develop a delivery system to send people and cargo into space. Falcon 1 (shown above), made its first successful flight 11 months ago. In December, SpaceX won a $1.6 billion Commercial Resupply Services contract to resupply the International Space Station. The company plans to demonstrate its docking capability next year. Stevens said it is important to wait and see if SpaceX can successfully deliver cargo before talking about delivering humans.
"We're concerned these entrepreneurial firms are promising too much. If you don't know what you don't know, then it's easy to say you can do this for so much," Stevens said.
Stevens raises some valid points, but he's also got a clear agenda – SpaceX and other firms like it are competitors and ultimately could do the job faster, cheaper and better than NASA. The Orion program is unlikely to make it to the moon any time soon based on current budgets projected in the future. The review committee says the goal of getting back to the moon by 2020 is currently about $30 billion short. And unless an extra $3 billion a year is put back in to the NASA manned space budget, the International Space Station is likely to be the only destination in space for the United States for the foreseeable future.
Naturally Musk, Burt Rutan and many others think otherwise. If they can do it, why shouldn't they?
UPDATE Aug. 21: We changed the headline, since the original confused some readers.
Photo of the Falcon 1 during liftoff from Omelek Island, U.S. Army Kwajalein Atoll, in the Central Pacific* on July 14 : SpaceX


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The Nielsen ratings at Halloween time usually bring out the network broomsticks. These are the key Nielsens following, as they do, sufficient exposure of the new season's programming.* After studying the results, the networks make their go-no-go decisions. This season the decisions are just a little more difficult to make, since many specialspolitical, entertainment, the Olympicspre-empted regular shows.
Altogether, the new Nielsens indicate that the strongest shows are from last year's schedules. Julia is the only newcomer that cracked the top ten. The list:
1) Rowan and Martin's Laugh-In (NBC)
2) Mayberry R.F.D. (CBS)
3) GomerPyleU.SM.C. (CBS) and Julia (NBC)
5) Family Affair (CBS)


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3/8" or 11mm black picot elastic with decorative edge. Decorative edge elastics can be used in lingerie design. Product is cut to order.
Effective immediately, no returns will be accepted for cut elastic due to possibility of exposure to Covid-19.
Wholesale Options
For larger bulk options, the minimum for this item is 5000 yards, has a 6-8 week lead time and requires a 50% deposit when the order is placed. Larger bulk orders are not eligible for accumulating Reward Points. Pricing by request.
© Copyright 2019 Fairbanks Publishing LLC DBA Porcelynne


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STAT5B gene
signal transducer and activator of transcription 5B
The information on this page was automatically extracted from online scientific databases.
From NCBI Gene:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008]
From UniProt:
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.
Covered on Genetics Home Reference:
From NCBI Gene:
- Laron syndrome with immunodeficiency
From UniProt:
Growth hormone insensitivity with immunodeficiency (GHII): A disease characterized by short stature, growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, resistance to exogeneous growth hormone therapy, and recurrent infections. [MIM:245590]
Cytogenetic Location: 17q21.2, which is the long (q) arm of chromosome 17 at position 21.2
Molecular Location: base pairs 42,199,177 to 42,288,437 on chromosome 17 (Homo sapiens Updated Annotation Release 109.20200522, GRCh38.p13) (NCBI)


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When I resumed the blog I wrote it would be temporarily. I can be more productive now, and the blog is only means for procrastination, therefore I will suspend it, until I have nothing better to do or I need to say something.
Tag: politics
Everything should have a form to open an issue
I have posted about a number of problems in society, economy, and politics, and I have suspended a number of posts along those lines. In this meta-post I try to do a pragmatic analysis.
The loss of my optimism
Apologies to anyone that had to deal with it in one way or another, I know it is not pleasant, and I have realized that contrary to Cunningham's Law, I was not getting arguments for optimism in return.
The Salk dilemma
The Salk dilemma occurs in faulty markets that are optimized to allocate rival goods in win-win scenarios, resulting in win-lose or lose-win scenarios for non-rival goods.
The technological singularity is beyond human capabilities
As humans we can evolve and become something else, or we can become extinct without spawning new species, but there is something that we cannot do: create a technological singularity.
The human brain is an ape brain, not a computer.
Consider the number of cognitive biases, how the important decisions belong to the "heart" not the brain. Human reason is nothing when compared with human rationalization.


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During embryogenesis, the myocardial layer of the primitive heart tube grows outward from the endocardial-lined lumen, with new cells added to generate concentric thickness to the wall. This is a key evolutionary step, demarcating vertebrates from more primitive chordates, and is essential for normal cardiac function. Zebrafish embryos with the recessive lethal mutations santa (san) and valentine (vtn) do not thicken, but do add the proper number of cells to the myocardium. Consequently, the heart chambers are huge, constituted of a monolayered myocardium lined by endocardium. This phenotype is similar to that of the heart of glass (heg) mutation, which we described previously as a novel endocardial expressed gene. By positional cloning, we here identify san as the zebrafish homolog of human CCM1, and vtn as the homolog of human CCM2. Dominant mutations of either in humans cause vascular anomalies in the brain, known as cerebral cavernous malformations. The synergistic effects of morpholino pairs indicate that san, vtn and heg are in a genetic pathway, and san and vtn contain protein motifs, NPxY and PTB domain, respectively, known to interact. This suggests that concentric growth of the myocardium, crucial for blood pressure generation, is dictated by a heg-san-vtn signaling pathway.
A central question of developmental biology is how vertebrate organs acquire their form. For the heart, part of such morphogenesis reflects control of overall cell number (Rottbauer et al., 2001; Rottbauer et al., 2002). Others control development of cells along two axes: anterior-posterior (Stainier and Fishman, 1992) and concentric (Mably et al., 2003). During the first stage of primitive heart tube formation the heart grows in essentially an anterior-posterior direction, with each of the two chambers constituted by a single-layered myocardium around the single layer of endocardium. The onset of concentric growth is marked by the addition of new cells in the myocardium in a direction perpendicular to the lumen, an outward growth that thickens the wall in a concentric direction, especially in the ventricle.
We have identified three mutations that block concentric growth without affecting overall cell number or the organization of anterior-posterior growth. We recently cloned one of these, heart of glass (heg), which turned out to be a novel gene, expressed in the endocardium. Here we focus on the other two genes, santa (san) and valentine (vtn). By positional cloning, we identify san as the zebrafish homolog of human CCM1 (KRIT1) (Laberge-le Couteulx et al., 1999; Sahoo et al., 1999) and vtn, the zebrafish homolog of human CCM2 (Denier et al., 2004). Mutations in these genes in humans have been implicated in the autosomal dominant disease, cerebral cavernous malformations (CCM).
Evidence from potential interacting protein motifs, and from cross-sensitization of phenotype though morpholino injection, suggests that san, vtn and heg may interact. This suggests that concentric growth of the ventricle is an essential element of cardiac patterning, controlled at least in part by signals from the endocardium, and involving a pathway comprised of san, vtn and heg.
Histological sectioning and cell counting
Histology was performed on paraformaldehyde-fixed embryos embedded in plastic (JB-4, Polysciences, Inc.). Sectioning was performed using a Jung supercut 2065 at 5 μm setting.
The zebrafish cardiac myosin light chain-2 (cmlc2) promoter-DsRed (red fluorescent protein) line has been described previously (Mably et al., 2003). Transgenic cmlc2:DsRed2-nuc zebrafish were bred with san heterozygotes. The progeny were raised and incrossed to identify san heterozygotes expressing red fluorescent protein (RFP). The embryos from these clutches were scored for the san phenotype. Wild-type siblings and mutant embryos were raised at 28.5°C until 48 or 72 hours post fertilization (hpf), at which time the embryos were flat-mounted and RFP-positive myocardial cells were counted (Mably et al., 2003; Shu et al., 2003). Morpholino-injected transgenic cmlc2:DsRed2-nuc embryos were analyzed in a similar manner. The same flat-mount technique was used to determine endocardial cell number in morpholino-injected transgenic (fli1:nEGFP)y7 embryos (Roman et al., 2002). The nuclear localization of each fluorescent protein facilitates easier determination of cell number.
Positional cloning
Embryos were separated into mutant and wild-type pools based on phenotypic analysis. Genomic DNA was isolated from individual embryos by incubation in DNA isolation buffer overnight at 50°C (DNA isolation buffer: 10 mmol/l Tris-HCl, pH 8.3; 50 mmol/l KCl; 0.3% Tween-20; 0.3% Nonidet P40; 0.5 mg/ml proteinase K). Proteinase K was inactivated before PCR setup by heating samples to 98°C for 10 minutes. PCR reactions were performed using diluted genomic DNA as described (Knapik et al., 1996). Bulked segregant analysis (Michelmore et al., 1991) and identification of the critical genetic interval was performed essentially as described previously (Mably et al., 2003). To identify the san gene, bacterial artificial chromosome (BAC) clones 92i12 and 184d07 (see Fig. S3 in the supplementary material) were sequenced by shotgun cloning of partial AluI and Sau3AI digested fragments subcloned into pBluescript. Sequence analysis was performed on an ABI3700 to generate approximately fivefold coverage. The sequence was assembled using the Phred/Phrap/Consed programs (Ewing and Green, 1998; Ewing et al., 1998; Gordon et al., 1998). The vtn gene was identified through morpholino analysis of genes identified as candidates by position through synteny with the Takifugu rubripes (fugu) genome, followed by sequencing of cDNA and genomic DNA from mutant and wild-type embryos.
RNA isolation and real-time PCR analysis
RNA was isolated using trizol (Invitrogen) or RNeasy columns (Qiagen) as instructed by the manufacturer. For determination of the mRNA transcript variants induced by the splice site blocking morpholinos, the QIAGEN OneStep RT-PCR (reverse transcriptase polymerase chain reaction) kit was used with primers designed from exons on either side of the morpholino target. The PCR primers used to detect the splice variants induced by the various morpholinos are summarized as follows.
san exon 1 donor: san_exon01_F1, 5′-AAAGAGGAGCTGCATGATGG-3′; san_exon02_R1, 5′-ATATGGGCTTGGTGGTTTCA-3′
san exon 14 donor: san_exon12_F1, 5′-TAGCCTCCTCCTGCAGATCA-3′; san_exon16_R1, 5′-CTTCATCAGCAGCTTCACGA-3′
vtn exon 2 donor: vtn_exon01_F1, 5′-TGAAGAGCATTTGTACGTAGAG-3′; vtn_exon04_R1, 5′-TCTCTGATGTAGGACACAGC-3′
Analysis of mRNA levels in the san wild type and -/- (mutant) embryos was performed using the Qiagen QuantiTect® SYBR® Green RT-PCR kit, as described by the manufacturer (Qiagen). The primers used for this analysis were designed to exon 13 (5′-GAGCAAAGCACATCACTGGA-3′) and exon 14 (5′-ATCACCTTGTGTGTGCTGGA-3′).
DNA cloning and RNA rescue
For RT-PCR analysis, RNA was isolated (RNeasy columns, Qiagen) from pools of wild-type and mutant embryos. cDNA was amplified using RACE (SMART RACE cDNA amplification kit, Clontech). Fragments were then subcloned into PCRII-TOPO (Invitrogen). The 5′ end of the san cDNA was amplified by 5′ RACE with a primer designed from the full-length cDNA predicted by Genscan (Burge and Karlin, 1998) (5′-TTCAGCAGGTTGGGGTTACAGTTGC-3′). A 3′ RACE product was generated using a primer (5′-TCTCAGTCAAACAGCTGGACAGCGAC-3′) designed to amplify an overlying fragment of the san cDNA. Both cDNA fragments were digested with SphI (a unique restriction site within the overlapping region) and EcoRI then ligated into EcoRI-digested pCS2 vector (Turner and Weintraub, 1994) to create the full-length san cDNA construct. Clones with the correct orientation were identified by sequencing (GenBank Accession Number DQ677877).
The full-length vtn cDNA was amplified using primers designed to the 5′ and 3′UTR sequences within the Genscan predicted cDNA (5′UTR_F1, AATACAGCGAAAATGAAGAGCA; 3′UTR_R1, CAGCATCCAAACTTTCAGCA). The PCR product was subcloned into pCRII-TOPO (Invitrogen). The vtn cDNA was excised from pCRII-TOPO by digestion with EcoRI, and then subcloned into EcoRI-digested pCS2 (Turner and Weintraub, 1994). Clones with the correct orientation were identified by sequencing (GenBank Accession Number DQ677878).
Injection RNA was generated from the full-length pCS2 san and vtn constructs using the Ambion mMESSAGE mMACHINE kit (digested with NotI followed by transcription with SP6 polymerase).
Whole-mount in-situ hybridization and antibody staining
For whole-mount in-situ hybridization and immunohistochemistry, embryos were fixed in 4% paraformaldehyde in phosphate-buffered saline, and then stored in 100% methanol at -20°C. Digoxigenin-labeled antisense RNA probes were generated by in-vitro transcription (Roche), and in-situ hybridization was carried out as previously described (Jowett and Lettice, 1994; Mably et al., 2003). The san probe used was derived from a partial EST in pSPORT1, fb36f07 (GenBank accession: AI415912, digested with EcoRI followed by transcription with SP6 polymerase). The vtn probe was derived from the full-length pCS2 construct described previously (digested with BamHI followed by transcription with T7 polymerase). Embryos were allowed to develop in BM purple (Roche) at 28°C, then stopped by several rinses in 1XPBT and stored at 4°C.
Antibody staining with the S46 and MF20 antibodies (Developmental Studies Hybridoma Bank, University of Iowa) was performed as previously described (Mably et al., 2003; Yelon et al., 1999).
Morpholino analysis
The antisense morpholino oligonucleotide designed over the san exon 1 donor site [5′-GCTTTATTTCACCTCAC(intron-exon)CTCATAGG-3′, GeneTools, LLC] was dissolved at a concentration of 200 μmol/l in 1× Danieau's buffer [5 mmol/l Hepes pH 7.6, 58 mmol/l NaCl, 0.7 mmol/l KCl, 0.6 mmol/l Ca(NO3)2, 0.4 mmol/l MgSO4]. One nanoliter of this solution or 1× Danieau's buffer was injected into each 1-4 cell embryo before allowing the embryos to develop at 28.5°C. The analysis with the san exon 14 donor site morpholino [5′-TTGAAGTCTCAC (intron-exon)TTTTGTCTCCATG-3′, GeneTools, LLC] and vtn exon 2 donor site morpholino [5′-GAAGCTGAGTAATAC(intron-exon)CTTAACTTCC-3′, GeneTools, LLC] were performed in the same manner.
santa and valentine exhibit defects of the myocardium and cardiac cushions
Both santa and valentine were identified from a large-scale zebrafish genetic screen, as N-ethyl-N-nitrosourea (ENU)-induced recessive embryonic lethal mutations with massively dilated hearts (Chen et al., 1996; Driever et al., 1996; Stainier et al., 1996). These mutants are unable to generate blood circulation, despite the presence of cardiac contractions. They are distinguishable from their wild-type siblings by 28-30 hpf on this basis, and later by the onset of chamber dilation. The cardiac chambers are dramatically enlarged by 48 hpf.
By contrast to the wild type, in which the myocardial wall is 2-3 cells thick (Fig. 1A), san and vtn both had only a single layer, in both chambers of the heart (Fig. 1C,E). Cells within the myocardium of both chambers of the heart were differentiated as cardiac cells, indicated by labeling with molecular markers for the atrium (S46 antibody) and ventricle (ventricular myosin heavy chain, vMHC; Fig. 1B,D,F) (Yelon et al., 1999). Both the endocardial and myocardial layers of the heart were present and individual myocardial cells were thinner than wild type (Fig. 1C′,E′).
By 48 hpf, wild-type zebrafish embryos develop valvular precursors, endocardial cushions, at the atrioventricular junction (Hu et al., 2000). Endocardial cushions were absent in san and vtn mutant embryos, as determined by neuregulin staining (Milan et al., 2006), and the myocardial and endocardial layers, which were very thin, were closely juxtaposed (Fig. 1; see Fig. S1 in the supplementary material). Electron microscopic (EM) analysis of the structure of cardiomyocytes within the ventricles of san and vtn hearts revealed the presence of sarcomeres, consistent with the ability of the hearts to contract (see Fig. S2 in the supplementary material). The absence of endocardial cushions and the variations in cellular morphology detected by EM could both be secondary effects of the severe cardiac dilation.
Myocardial and endocardial cell number is normal
The massive enlargement of the san and vtn hearts could be suggestive of an increase in cell number. To determine cell number, we specifically labeled cardiomyocytes in vivo, using transgenic zebrafish with a red fluorescent protein (DsRed2-nuc) expressed under the control of the cardiac myosin light chain-2 (cmlc2) promoter (Mably et al., 2003; Rottbauer et al., 2002). This assay indicated that the number of myocardial cells was indistinguishable between wild-type and san mutant embryos and between mock-injected and vtn morphant embryos at 2 days post fertilization (dpf) (Table 1). Similarly, we determined the number of endocardial cells in mock-injected and san and vtn morphant embryos at 2 dpf (Table 1). Morpholinos were injected into the progeny of transgenic (fli1:nEGFP)y7 crossed with transgenic cmlc2:DsRed2-nuc zebrafish. The number of eGFP-expressing cells in the hearts of progeny expressing both the myocardial RFP and endothelial/endocardial eGFP were determined. As noted for the myocardial cell counts, endocardial cell number did not vary from that determined for wild type. Hence, the dilated heart is not caused by an increase in the number of cardiac cells but rather in the manner they are assembled. The myocardial cells stretch in a single layer along the circumference of the cardiac chambers, rather than intercalating to form a thick myocardial wall, resulting in the observed chamber dilation.
Characterization of the san and vtn genes
We positionally cloned both san and vtn (see Fig. S3 in the supplementary material). The complete san gene is comprised of 16 coding exons with a deduced amino acid sequence of 741 amino acids. The human homolog is krit1 (CCM1) (Fig. 2A). The protein is characterized by the presence of several protein domains, including two NPxY motifs (residues 191-194 and 229-232), three ankyrin repeats (residues 285-317, 318-351 and 352-385), and a C-terminal B41/FERM domain (residues 414-638).
The N-terminal NPxY motif is conserved in vertebrate species examined and also in the Caenorhabditis elegans ortholog (ZK265.1). This motif has been shown to interact with the phosphotyrosine (PTB)-containing protein ICAP1α (Zawistowski et al., 2002; Zhang et al., 2001). In C. elegans this sequence is NPXF (Fig. 2A), similar to the elements within the intracellular domain ofβ 2-integrins (Calderwood et al., 2003). The Y→F substitution results in a motif that can still interact with a PTB domain but is not subject to regulation by phosphorylation state (Calderwood et al., 2003). The three ankyrin repeats, believed to be sites of protein-protein interactions (reviewed by Mosavi et al., 2004), and the FERM domain (reviewed by Bretscher et al., 2002), implicated in the association of proteins with the cell membrane, are conserved across species, although the sequence of the FERM domain in the C. elegans homolog is poorly conserved and truncated (Fig. 2A).
Sequencing of the m775 (Stainier et al., 1996) san cDNA predicts a splicing defect with a consequent in-frame deletion of exon 14. This results from a splice acceptor mutation within the intron at the start of exon 14 (agAG→aaAG; Fig. 2A). The affect on the level of full-length san message was confirmed by real-time PCR amplification of m775 mRNA, showing significantly decreased levels of RNA transcripts containing exon 14 (Fig. 2B). In addition we sequenced another san allele, ty219c (Chen et al., 1996). This mutation is a C to A transversion within codon 694 of exon 15 (TAC→TAA) that predicts a tyrosine change to a stop codon (Y→stop; Fig. 2A). Both mutations would be predicted to cause loss of a C-terminal portion of the Santa protein, possibly disrupting function of the FERM domain.
The complete vtn gene is comprised of 10 coding exons with a deduced amino acid sequence of 455 amino acids. The predicted protein is cytosolic with a molecular weight of 50 kDa. The protein has one recognizable protein motif, a PTB domain (amino acids 61-229). The vtn protein is highly conserved across vertebrates and encodes the zebrafish homolog of the human gene associated with CCM2 (MGC4607, malcalverin; Fig. 2C). The m201 allele of vtn (Stainier et al., 1996) is defined by a C to A transversion within codon 119 of exon 4 (TAC→TAA). This would cause a tyrosine change to a stop codon (Y→stop, Fig. 2C). This results in the formation of a truncated protein with an incomplete PTB domain.
Morpholino confirmation and RNA rescue of genetic lesions
We employed an antisense morpholino designed to block splicing at the donor site at the end of exon 14 of the san mRNA (data not shown) (Draper et al., 2001; Ekker, 2000; Mably et al., 2003). Injection of this morpholino at the 1-cell stage resulted in a complete copy of the mutant phenotype (>95% of embryos show the phenotype, n>1000). Analysis by RT-PCR using primers within exons flanking exon 14 revealed a partial in-frame loss of exon 14 sequence, confirming that loss of part of the FERM domain is sufficient to reproduce the mutant phenotype (data not shown). Mock-injected controls reveal no effect on the san PCR products.
We also designed a morpholino to block splicing of the donor site at the end of exon 1 in an attempt to create a truncated protein (through the excision of 5′ exon sequence and disruption of the reading frame). However, this morpholino caused deletion of 75 base pairs (bp), predicting in-frame loss of 25 amino acids, due to the recruitment of a splice donor site within exon 1 (data not shown). Interestingly, loss of these 25 amino acids was sufficient to produce a complete phenocopy, implicating the N-terminus of the protein as essential for normal function. Injection of mRNA derived from the predicted full-length san cDNA was unable to rescue the mutant phenotype. At high levels, expression of san mRNA in wild-type embryos impaired early development, probably due to misexpression of San in all cells.
A morpholino was designed to the donor site at the end of exon 2 of the vtn gene. Injection of this morpholino at the 1-cell stage results in a complete phenocopy of the vtn mutation (>95% phenocopy with n>1000, data not shown). Analysis by RT-PCR using primers within exons flanking exon 2 and sequence analysis of the products revealed transcripts predicting both a partial and complete loss of exon 2 sequence (data not shown). Injection of predicted vtn mRNA into progeny of vtn heterozygote matings rescued embryos completely (Table 2).
san and vtn mRNA expression
Analysis of san mRNA by whole-mount in-situ hybridization at 28 hpf revealed staining in the ventricular zone of the brain (Fig. 3A,B), with weaker staining throughout the entire brain, and in the posterior cardinal vein (Fig. 3C,D). The venous expression was maintained at 48 hpf, although expression was patchy (Fig. 3E,F). At 48 hpf there was also prominent notochord staining.
Similarly, strong expression of vtn mRNA at 28 hpf was detected in the brain ventricular zone (Fig. 4A-D) with weaker expression in the vein (Fig. 4E,G). vtn mRNA was also obvious in the posterior intermediate cell mass at this stage (Fig. 4A,E,F). By 48 hpf, expression of vtn was weaker, but was detectable in the vein (Fig. 4H-J). At both 28 and 48 hpf, a low level of vtn mRNA was detectable in a region near the dorsal aorta (Fig. 4G,J).
Co-morpholino evidence of interaction of san, vtn and heg
The similarity of phenotype between san, vtn and heg, along with the predicted interactive ability of protein motifs (PTB domain of vtn and NPxY motif of san/NPxF motif of heg) suggested that these proteins might be part of a pathway that controls concentric growth of the heart. To examine this, we lowered amounts of each protein by morpholino injection to a level at which fewer than 10% of embryos demonstrated a complete phenocopy. Combinations of morpholinos were then injected at these doses to determine if the effects of these morpholinos are additive. Injection of san and vtn together at these doses resulted in a dramatic increase in the percentage of embryos exhibiting complete phenocopy (Fig. 5; see Table S1 in the supplementary material). When injected together with either the san or vtn morpholino at low doses, the heg combinations also produce a significant increase in phenocopy level (Fig. 5). These results suggest that san, vtn and heg interact.
The thickening of the myocardium is essential to generate the higher blood pressures characteristic of vertebrate physiology. The primitive heart tube forms before physiological function, suggesting that the primary determinant of the tube's initial dimensions are not dictated by physiological forces. In fact, there is evidence that the size of the heart correlates with overall body growth (termed allometry) (Gould, 1966). After transplantation of precardiac mesoderm between two amphibian species, tissue from the faster growing Amblystoma tigrinum transferred to Amblystoma punctatum results in hearts that grow more rapidly than in the native host (Copenhaver, 1939). Flow and pressure clearly modify subsequent patterns of growth in the embryonic ventricle, as illustrated by the formation of congenital defects in chick embryos following constriction of the outflow tract during development of the heart tube (Gessner, 1966).
Through the characterization of two zebrafish mutants, san and vtn, we introduce here a new genetic pathway, one essential for myocardial growth and thickening of the ventricular wall in vertebrates. In the hearts of these mutants, both atrial and ventricular chambers are correctly specified and hearts possess myofibrils and are able to contract. Although the number of cells in the heart muscle wall is normal, there is no concentric growth. Rather, the cells proliferate along the circumference of the heart, leading to the formation of a hugely dilated heart consisting of a single layer of myocardium. The targeted deletion of the homolog of san in mice also results in an enlargement of the cardiac chambers, particularly the atrium (Whitehead et al., 2004). However, the hearts loop normally and develop endocardial cushions (Whitehead et al., 2004). The absence of looping and the loss of endocardial cushion formation in the zebrafish mutant may represent secondary affects on the morphology of the heart resulting from the severe cardiac dilation observed or possible variations in timing requirements between species for these genes during development.
CCM1 (San) interacts with integrin cytoplasmic domain associated protein-1 (ICAP-1), a modulator of integrin signaling (Zawistowski et al., 2002; Zhang et al., 2001). Like Vtn (CCM2), this protein contains a PTB domain. This PTB domain is essential for the interaction of ICAP-1 with the NPxY motif within the cytoplasmic tail ofβ 1 integrin (Chang et al., 1997; Zhang and Hemler, 1999) and with the NPxY domain of CCM1 (amino acids NPAY, see Fig. 2A). These interactions with CCM1 and/or β1 integrin have been proposed to modulate cell-cell and cell-extracellular matrix communication, essential for a variety of processes, including cell migration and maintenance of cellular morphology.
Consistent with the morpholino data indicating that san, vtn and heg interact to control myocardial thickening in the zebrafish heart during development, recent work has demonstrated a physical interaction between CCM1 (San) and CCM2 (Vtn) by immunoprecipitation and FRET analysis (Zawistowski et al., 2005). Unlike the interaction between ICAP1 and CCM1, binding to CCM2 was not completely eliminated by mutagenesis of the N-terminal NPAY motif in CCM1, although this motif was shown to be involved in CCM2-binding (Zawistowski et al., 2005). These results suggest that CCM2 can bind to additional motifs in CCM1. However, mutagenesis of the other two NPxF/Y motifs in CCM1 (indicated in Fig. 2A) revealed that they were not strong sites of CCM2 interaction (Zawistowski et al., 2005). In addition, the murine CCM2 homolog (OSM, for osmosensing scaffold for MEKK3) serves as a scaffold protein for MEKK3-mediated p38 MAPK phosphorylation during osmotic shock, interacting directly with Rac1, MEKK3 and MKK3 (Uhlik et al., 2003). This complex also includes CCM1 (San), as demonstrated by co-immunoprecipitation (Zawistowski et al., 2005).
The results of our co-morpholino experiments also implicate Heg as another member of this complex interactome. The heg gene has been shown to be an endocardial signal required for the concentric thickening of the myocardium. Loss of function of this gene in zebrafish generates a phenotype indistinguishable from san and vtn (Mably et al., 2003). We have demonstrated that knockdown of combinations of the three zebrafish genes show synergistic effects, strengthening the contention that the genes disrupted in human CCM function through a common molecular pathway.
The phenotype of the san and vtn mutant embryonic heart, like heg, most closely resembles that of the heart in cloche (clo) mutant zebrafish, which lack endocardium and other endothelium (Liao et al., 1997; Parker and Stainier, 1999; Stainier et al., 1996). Based on the expression pattern of the san and vtn genes, it is difficult to conclude which of the two cell types in the hearts is primarily affected, although the expression of san and vtn in the vasculature and heg in the endocardium (Mably et al., 2003), together with the similarity to the clo phenotype, suggest a role for endocardial cells in growth of the adjacent myocardium. The dilated cardiac chambers of the san and vtn mutants exhibit a severe thinning and dilation that is also reminiscent of the dilated capillary walls observed in PDGF-B-deficient mice, which exhibit impaired recruitment of pericytes because of the loss of PDGF-B signaling (Lindahl et al., 1997). It is postulated that one of the roles of the pericytes is to contribute to the mechanical stability of the capillary wall, and in their absence microaneurysms develop. Analogous to these lesions, the enlarged cardiac chambers of the san and vtn mutants reflects an inability of the endocardial cell layer to instruct the adjacent layer of myocardial muscle to thicken.
During the initial stages of embryonic development, the endocardium may help maintain the structural integrity of the heart by serving as a framework for the surrounding myocardium. Furthermore, although the number of endocardial cells in the two mutants is similar to wild type, a small population of cells may be recruited to the endocardium from other sites, such as the intermediate cell mass (ICM), where vtn is expressed, and these cells may migrate through the vasculature to contribute to the maturation of the endocardium. Indeed, the endocardium has been proposed to be the site of hematopoiesis before onset of definitive hematopoiesis in the kidney at 4-5 dpf seeded by cells from the blood islands within the ICM (Al-Adhami and Kunz, 1977). The expression of both san and vtn in the vein may represent this migrating population of cells that are essential to maturation of the endocardium and, indirectly, the adjacent myocardium through the supply of growth signals.
The human homologs of the zebrafish san (CCM1) and vtn (CCM2) genes are disrupted in cerebral cavernous malformations, a disorder characterized by the formation of enlarged and thin-walled vascular malformations in the CNS (Gil-Nagel et al., 1995; Zabramski et al., 1999). The mechanism by which disruptions in these genes produce these characteristic venous lesions is undefined. However, a similar process to that proposed to explain the zebrafish defects might lay at the basis of these vascular anomalies as well as the defect in the mouse CCM1 (san) knockout, which reveals an essential role for this gene in vascular development (Whitehead et al., 2004).
This work reveals the molecular basis for a mechanism of cardiac growth distinct from those that control cell proliferation. This process determines the direction of growth and is essential for the thickening of the ventricular wall. Formation of the myocardium is contingent on the presence of an intact endocardium and requires reciprocal signaling between the two cell types, facilitated through a genetic module consisting of san, vtn and heg.
Supplementary material
Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/133/16/3139/DC1
We thank C. Simpson for histological sectioning, and B. Roman, N. Lawson and B. Weinstein for the transgenic (fli1:nEGFP)y7 zebrafish. We thank Joanne Chan for critical review of this manuscript. This work was supported by grants from NIH [5R01HL49579,5R01DK55383 (MCF) and 5R01HL63206-04 (JDM/MCF)], and by a sponsored research agreement with Genentech.
↵† Present address: The Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA
↵‡ Present address: Case Western Reserve University, 11119 Bellflower Road, 150 PBL, Cleveland, OH 44106-7235, USA
↵§ Present address: Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Life Sciences Building, Rm 5109, 621 Charles E. Young Drive South, Los Angeles, CA 90095, USA
- Accepted June 1, 2006.
- © 2006.


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Adbank ICO Fixes The Broken Advertising World
Adbank Network ICO is bringing new dimensions to the advertising industry, mainly the online adverts networks. Adbank ICO is live now and will be over by 21 January 2018. They have nearly sold 75% of the tokens in just few days. They are creating a… Read More


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Olympic Embarrassment: Steroid Double Standard
An article in the November 2, 2005 edition of The New York Times points out the hypocrisy of Olympic and world anti-doping officials regarding the Italian anti-doping law. "Steroid Laws: Equal Justice And Punishment for All," by Selena Roberts, offers much food for thought [abridged version]:
Steroid Laws: Equal Justice and Punishment for All
by Selena Roberts
Who is the role model for criminal behavior?
There is a 40-something health-club barfly who wakes up with mirrors over his bed and sleeveless T-shirts in his closet. The guy owns a tackle-box full of steroids that he purchased over the Internet in hopes of bulking up just enough to pick up the ladies.
There is an elite athlete who wakes up with mirrors in his home gym and a closet full of lucrative endorsement deals. The sports star has a paid trainer who administers steroids so he can find an edge to shatter records and gain wealth.
Where does a police raid take place — health club or clubhouse? Whose home is ransacked by the police — the gym rat's or the sports star's?
"It's the truck driver, the guy working at the Stop & Shop — as narcissistic or misguided as they might be," said Rick Collins of Long Island, who specializes in steroid law. "These are the lives destroyed by the steroid laws." Collins's clients are not sports icons, because athletes haven't been the targets of steroid possession laws, even though their using prompted the Anabolic Steroid Act of 1990 and 2004.
Players are the cause of steroid criminalization by the Beltway gang, but aren't treated as criminals. Instead, politicians treat them to scoldings and threats and timeouts in the corner. Once again yesterday, members of Congress feeling frustrated by the inaction of pro leagues in strengthening antidoping policies reintroduced Olympic-style legislation to standardize testing and toughen suspensions. Suspensions are not based on the legal system, but on a morality code for elite athletes who are idolized by malleable youth.
"It's political," Collins said. "It's easier to go after the regular guy rather than expose our heroes."
Sports icons are not granted immunity everywhere. Italy is one example. And this scares the O-rings off the International Olympic Committee just 100 days before the Winter Games in Turin. The same I.O.C. leaders who trumpet zero tolerance for drug cheating spent last week pleading in futility for Italian authorities to relax their punitive antidoping laws, which send offenders to jail for three months to three years. "It is a question of sporting ethics," the I.O.C. president, Jacques Rogge, said Friday, "rather than a question of crime and criminality."
If true, then why did Dick Pound, I.O.C. member and chairman of the World Anti-Doping Agency, rip into the light prison sentence Victor Conte, steroid designer to the stars, received in the Balco case? In one reference, Pound called Conte's plea deal a potential "cop-out on a cosmic scale."
Pound has spent a career mocking cheating athletes for blaming dastardly opponents and tainted supplements for the fly in their soup. But now he is offering his ample breath to conspiracy theories, openly fretting about potential setups by saboteurs who would lure police officials to a competing athlete's steroid stash.
Why the use of selective paranoia and desire for boutique-style justice? It goes to self-preservation. First, the I.O.C. doesn't want its pristine Winter Olympics tarnished by images of pixie skaters removed from the ice in leg irons or of snowboarders escorted from the halfpipe in handcuffs. Second, the I.O.C. can't fathom losing control over its antidoping program after pouring years into the creation of the W.A.D.A. All that time creating a uniform drug standard. All that effort to get every national Olympic committee on board. And now the W.A.D.A. is irrelevant? And now the police want to police? "The Italian law criminalizes sports cheating," Collins said. "It hits the intended target."
No one wants to see athletes forced to turn in their U.S.A. berets at the police desk. No one wants to see a sports figure working out in a prison yard. … But shouldn't a doping violator — whether he is in the N.F.L. or whether she is a figure skating star — have to answer to authorities? How about answering one question: Who is your supplier? This is not about the steroid law itself, but about the equal application of it for everyone, from the anonymous store clerk hooked on vanity to the visible sports star hooked on glory. "It's incredibly hypocritical," Collins said. "It's a bait-and-switch. The very people the laws were enacted to apply to are now asking to be exempt. There is something wrong with that. The antidoping officials pushed for tougher laws as long as the laws didn't affect the athletes. "I can't think of one elite athlete who has been prosecuted on steroid possession. There is clearly a disconnect." This disconnect is in the mirror of the steroid user. One reveals an athlete, the other a criminal.


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Al Hijaz Restaurant
A spacious, all-day dining venue on F floor, Al Hijaz Restaurant features international, Turkish, Asian and Oriental buffets for breakfast, lunch and dinner.
Enjoy sublime salads and appetizers, superb soups, perfect pastas, delicious burgers, fabulous fish dishes, divine desserts and much more besides. Along with Um Al Qura Restaurant, 1,500 guests can be comfortably seated at once.


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Teignmouth harbour on a summer afternoon in June (the first)
A Greater Black Backed Gull is crunching on crab.
A Starling is furtling frantically under the seaweed.
(Sparrows are busybodying in)
A crow jumps in to finish off the Gulls crab leftover.
Lunching in the Teign. Easy pickings and peckings.
Words & Vid: Ian Nisbet


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|Aakar Se Nirakar Tak.We believe in Perfection. But unfortunately we all are imperfects. We believe in God but we don't believe in it's existence structure. But at every stage of life some unknown miracles, some unexpected eventualities make you believe in God. God is everywhere-present in every part of this universe, and so the "LOVE". Love and God are synonyms. And since Love is very deeply rooted into the services and such services are the base of Female Genetics so the Love-Services-Women and God somewhere in our Vedic Texts in India have been treated as Supreme. From God to Friend to daughter My love is dedicated to the supreme Services they all are providing to the mankind. I more believe in Imperfections. I am not perfect. Not even for a single moment I treat myself a perfect human identity. So I am learning and learning from people from nature and from God. I decided to visit Lord Shiva on my Intuitions to visit his unseen, unpredictable, non-existed world of Power, Energy, Strength, and supreme administrations. I know I could not but just on the instincts and call of God I move ahead on a journey with all my Imperfections. I got some intuitive words of lyrics, I compose it imperfectly and started singing imperfectly. As I started singing lot of people all imperfect who were around me on this journey started accompanying me with their rural and contemporary instruments of music with their dedication and devotion to GOD and Lord shiva. Even the Nature, natural sounds of flowing rivers, Blowing winds and chirping birds started getting more strong on such a peaceful serene and silent trends and path. Everyone pacing with me was creating developing and composing some sounds and music all imperfect because everyone wanted to approach God with that Imperfection to be perfect near God. No one including me tried to be perfect in my lyrics, music and instrumental sounds. And all imperfections together created an amazing imperfect musical lyrical songs devoted to Lord Shiva. Anyone who believe in dedication and Devotion , who believe in the path of imperfection to perfection shall listen to this lyrical music with original tracks and natural sounds. Anyone needing and deserving perfections in Music, lyric and instruments shall not listen this. This is not for your enjoyment but for bringing perfection near the door of Lord out of all your imperfections. Go may be there may not be there but at least God is always within us in our soul and heart. So this is also a journey towards your soul and spirit to recognize yourself. From Existence To Non Existence God is Everywhere. Lord Shiva is in every particle, in every molecule in every soul. Siva Shiva Shiva From Existence To Non Existence. Aakar Se Nirakar Tak-Lord Shiva
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Unfortunately popping a pimple is a bad habit we all have. I mean imagine having an unwanted thing on your face. Without even thinking about it, your finger is just drawn to it, ready to pick and flick it out off your face. But that habit has to be controlled, especially when it can cause serious damage to your face.
The best time to pop a pimple is when it has a white head, when all the puss in it has risen to the surface. if it's a cyst or cyst like bump, just give it time to erupt. Do not squeeze it... instead just walk away from the mirror and keep your hands and mind busy so it doesn't go to your face and you can distract yourself from thinking about it.
Squeezing and touching it can infect and inflame the tissue around the skin causing you to have a swollen, red bump that bleeds and then forms a scab over it, which makes it harder to cover it up. After a few days, you notice it has turned into a scar that then that takes months or even years to get rid of the scar marks. And we all know how hard it is to get rid of those black spots. I mean It's a constant battle getting rid of hyper pigmentation.
The best and correct way to pop a pimple so that you get no scars, no infection and you don't even need a dermatologist for it is by following the steps below;
Step One - Run Hot water on a clean wash cloth.
Step Two - Wring out the excess water in the wash cloth so it stops dripping.
Step Three - Place the wash cloth on the whitehead. While this opens up your pores, it brings all the puss to the surface. Keep the cloth on it until the washcloth cools off.
Step Four - Gently slide the wash cloth sideways, towards your ears. you should notice the whitehead sliding off with the wash cloth.
Step Five (if necessary) - If it didn't bring the white puss to the top, repeat the steps again. (Make sure its a white head before you try to pop it).
Step Six - Once you remove the whitehead, rinse your face with cold water to close your pores and pat dry with a clean dry wash cloth.
Step Seven - Apply an antibacterial cream such as benzoylperoxide cream, tee tree oil or any blemish treatment you like, and let the pimple dry out completely.
And VOILA, You successfully popped your pimple without a scar, blood, or anything that will damage your skin. Very easy steps that work well if you get it right.
Thanks for coming to my skincare blog. Leave in the comment box below, your favorite Blemish Topical Cream to get rid of acne.