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Bacillus subtilis exhibits a complex adaptive response to low levels of peroxides. We used global transcriptional profiling to monitor the magnitude and kinetics of changes in the mRNA population after exposure to either hydrogen peroxide (H2O2) or tert-butyl peroxide (t-buOOH). The peroxide stimulons could be largely accounted for by three regulons controlled by the PerR, σB, and OhrR transcription factors. Three members of the PerR regulon (katA, mrgA, and zosA) were strongly induced by H2O2 and weakly induced by t-buOOH. The remaining members of the PerR regulon were only modestly up-regulated by peroxide treatment. Overall, the magnitude of peroxide induction of PerR regulon genes corresponded well with the extent of derepression in a perR mutant strain. The σB regulon was activated by 58 μM H2O2 but not by 8 μM H2O2 and was strongly activated by either t-buOOH or, in a control experiment, tert-butyl alcohol. Apart from the σB regulon there was a single gene, ohrA, that was strongly and rapidly induced by t-buOOH exposure. This gene, controlled by the peroxide-sensing repressor OhrR, was not induced by any of the other conditions tested.
Treatment of bacteria with low levels of oxidants typically results in a classic adaptive response: the treated cells have a greatly enhanced ability to survive subsequent challenge with an otherwise lethal dose of the same oxidant or a related oxidant. This adaptive response is coordinated by the action of transcription factors that sense oxidative stress and regulate the expression of appropriate defensive and repair functions (34).
In Escherichia coli resistance to oxidative stress is largely coordinated by two systems. OxyR activates genes in response to either peroxide stress or changes in the thiol-disulfide status, while the SoxRS system controls genes in response to reactive free radicals, such as superoxide anion and nitric oxide (29, 35). Proteins induced by oxidative stress were initially catalogued by using one- and two-dimensional polyacrylamide gel electrophoresis (PAGE) (12, 15, 26). More recently, the OxyR and SoxRS regulons have been defined by using computational approaches to identify regulator binding sites together with transcriptional profiling (28, 39, 40).
Bacillus subtilis also displays an adaptive response to low levels of oxidants. Enzymatic assays have revealed that the major catalase present in growing cells (KatA) is strongly induced by treatment with peroxides (3, 25). With one-dimensional sodium dodecyl sulfate-PAGE several other proteins were also found to be strongly induced by 50 μM H2O2 (13). The most prominent members of the peroxide stimulon include the two subunits of alkyl hydroperoxide reductase (AhpC and AhpF), KatA, and the DNA-binding protein MrgA (9, 20). Similar conclusions were reached in studies of the protein profile of cells treated with H2O2, as visualized by two-dimensional PAGE (2).
Insight into the mechanisms controlling the inducible peroxide stress response in B. subtilis originally emerged from analysis of mrgA, which encodes a member of the Dps family of protective DNA-binding proteins (7, 9). Expression of mrgA is metalloregulated: repression is elicited by growth with either supplemental manganese or iron (8). This repression is mediated by PerR, a member of the ferric uptake repressor (Fur) family of metal-dependent DNA-binding proteins (5). The PerR regulon is now known to include mrgA, katA, ahpCF, a heme biosynthesis operon (hemAXCDBL), a zinc uptake system (zosA), fur, and perR itself (23). Studies with lacZ reporter fusions have demonstrated that expression of these genes is repressed by manganese and that some, but not all, of the genes are also repressed by iron (18). Some of these genes (mrgA, katA, and zosA) are strongly induced by H2O2, while others (hemA operon, ahpCF, and perR) show less induction. The fur gene is not induced by H2O2; thus, not all members of the PerR regulon are members of the peroxide stimulon (18).
To gain a global perspective on the transcriptional responses to peroxide stress, we monitored gene expression in stressed cells using DNA microarrays. Since many stress responses involve transient changes in gene expression, we isolated RNA at several times following imposition of stress and compared the resulting transcriptional profiles with the profiles of unstressed control cells. In addition, in this paper we describe a direct comparison between wild-type and perR mutant cells. A comparison of the responses to H2O2, tert-butyl peroxide (t-buOOH), and tert-butyl alcohol (t-buOH) and previously reported studies of the heat shock stimulon (22) revealed three distinct classes of induced genes: the PerR, σB, and OhrR regulons.
Strains and growth conditions. B. subtilis CU1065 (W168 attSPβ trpC2) was inoculated into 25 ml of prewarmed (37°C) Bacto Mueller-Hinton broth (MHB) (Difco, Detroit, Mich.) from a fresh (overnight) Luria-Bertani agar plate. The flask was shaken at 250 rpm on a rotary platform at 37°C until the optical density at 600 nm (OD600) was 0.5 to 1.0. This seeder flask was used to inoculate a second flask of prewarmed MHB (50 ml in a 500-ml flask) to an OD600 of 0.2. This flask was grown until the OD600 was 1.0, at which time aliquots were removed and used as the time-zero samples. For the H2O2 stress experiments, 100 μl of appropriately diluted H2O2 was added immediately following removal of a time-zero aliquot to give a concentration of either 8 or 58 μM. Water (100 μl) was added to a mock treatment control flask. For the organic peroxide stress experiments, t-buOOH or t-buOH was added to a concentration of 100 μM (total amount added, 100 μl). Each flask was incubated on the shaker, and samples used for RNA isolation were removed at the times indicated below. The initial sampling of a culture was performed rapidly so that cells were frozen in liquid nitrogen within 40 s of removal from the culture.
Growth of the perR mutant strain.Cultures were inoculated as described above by using aliquots from an MHB plate containing either CU1065 or an isogenic perR strain (5) that had been grown overnight. The perR inoculum contained only small colonies, which was indicative of a lack of pseudorevertants (6). Each flask was shaken at 250 rpm on a rotary platform at 37°C. Samples used for RNA isolation were collected at an OD600 of 1.0. Prior observation had shown that the cultures exhibited exponential growth at this cell density (data not shown). CU1065 reached an OD600 of 1.0 in ∼3 h, while a similar inoculum of the isogenic perR strain took ∼7.5 h to reach a similar cell density. After the samples used for RNA isolation were removed, the cultures were diluted and plated to obtain single colonies on MHB agar. Following overnight growth the perR strain plate was examined for large pseudorevertant colonies, and individual colonies were tested for catalase activity to confirm that the perR strain culture contained undetectable levels of catalase-negative pseudorevertants (6). RNA was isolated as described above.
Microarray analysis, cDNA labeling, slide hybridization, data collection, and normalization.The procedures used for microarray analysis, cDNA labeling, slide hybridization, data collection, and normalization have been described previously (22). Time-zero cDNA samples were labeled with Cy3, and samples collected at subsequent times were labeled with Cy5. Slides were scanned and analyzed with a confocal laser scanner and a software package (Axon GenePix 4000A and GenePix Pro 3.0; Axon Instruments, Inc., Foster City, Calif.). Normalization was computed by using the algorithm incorporated into the Stanford Microarray Database (33), and all data are archived in this database. In general, fold induction was determined by comparison of the peroxide-treated samples and the time-zero control samples. However, where indicated below, the experimental data were corrected for time-dependent changes in the samples by using data for the parallel untreated samples. For example, the log-transformed gene induction ratios after mock treatment were subtracted from the log-transformed induction ratios after H2O2 treatment. To identify and display groups of coregulated genes for this set of treatment regimens, we used hierarchical clustering as previously described (14). For the 8 and 58 μM H2O2 regimens, genes were considered to be differentially regulated if after 3, 10, or 20 min (i) the absolute value of the mean of the replicate log2 treatment ratios minus the mean of the log2 mock treatment ratios exceeded 1.585 (corresponding to a threefold difference) and (ii) significance analysis of microarrays (SAM) of all available treatment replicates versus mock replicates in a two-class, unpaired data design indicated statistical significance at the threefold level when the delta parameter was adjusted to give a median number of false significants typically less than 5% of the total number of selected genes but allowing for at least one false significant in a set regardless of its size (36). A similar analysis was carried out for the t-buOOH-versus-t-buOH experiments, with t-buOH being considered the mock treatment in the two-class design. In the perR mutant studies, reciprocal cDNA labeling was conducted with either CU1065 Cy3 versus perR Cy5 or CU1065 Cy5 versus perR Cy3. A total of six slides (three for each labeling regimen) were analyzed. In this case, two-class, unpaired data SAM was performed by using normalized intensity data rather than ratio data, and the delta parameter was adjusted to give a median number of false significants < 2. Supplemental tables and complete data sets are available at http://www.micro.cornell.edu/faculty.JHelmann.html .
Overview of experimental design.We monitored the global transcriptional profile of B. subtilis under peroxide stress conditions using DNA microarray-based measurements. To monitor changes in gene expression over time, we compared the mRNA profiles obtained 3, 10, 20, and 40 min after stress was imposed with the profile of a time-zero (prestress) control sample. Changes in gene expression were monitored for three different peroxide stress conditions: low (8 μM) and intermediate (58 μM) levels of H2O2 and 100 μM t-buOOH. Note that 58 μM H2O2 is designated an intermediate level of stress to distinguish our results from those of experiments performed by other groups with much higher levels of H2O2 (39). As controls we also monitored gene expression in parallel cultures treated with the corresponding alcohols (H2O and t-buOH). In addition, we compared the transcriptional profile of a strain with a disruption in perR with the profile of the corresponding wild-type control. The majority of comparisons were performed on at least three independent slides, and while the SAM was based on all available replicates, the geometric means of the experimental ratios were, for reasons of convenience, used to generate the figures.
H2O2 stress stimulon.To define the major groups of genes most affected by peroxide stress, we performed a cluster analysis using the software developed by Eisen et al. (14). For this analysis we clustered the data by using data sets obtained for induction by H2O2 at concentrations of 8 and 58 μM (corrected for changes in gene expression that occurred as a function of time in the parallel untreated cultures), by t-buOOH, and by t-buOH and for the changes noted in the perR mutant compared with data for the wild type. In addition, we included the previously described results obtained for the heat shock stimulon (22) to aid in clustering, although these data are not incorporated in Fig. 1.
The cluster analysis clearly grouped a subset of the PerR regulon genes as the genes that were strongly induced by both low and intermediate levels of H2O2 and were derepressed in the perR mutant (Fig. 1). Only four genes (ygbA, katA, zosA, and mrgA) were strongly and rapidly up-regulated by the low level of H2O2 (Fig. 1, third zoom box). Three of these genes are known members of the PerR regulon, whereas peroxide induction of ygbA has not been reported previously. The ygbA gene (also called ssuA) is the second gene in an operon involved in sulfonate transport that is transcribed divergently from the strongly induced katA gene (37). Since other genes in this operon are not peroxide inducible, the apparent induction noted here could have been an artifact (e.g., there could have been cross-hybridization or contamination of the ygbA PCR product with longer products extending into katA). A second, much larger cluster of genes was induced by the intermediate level of H2O2 but not by the low level of H2O2 and was further distinguished by strong and rapid induction in response to t-buOOH and t-buOH. This cluster corresponds to the large σB general stress response regulon (21, 27, 30, 31), and only a subset (ydaP though yjgC) is shown here (Fig. 1, fourth zoom box). Among the genes most dramatically down-regulated following imposition of peroxide stress are the genes comprising the PBSX phage-like element (xkdR through xtmA) (Fig. 1, first zoom box). Expression of these genes is also greatly reduced in a perR mutant (see below).
As an alternative method to visualize clusters of coregulated genes, we used a graphical approach to compare changes in gene expression under two different conditions. Since the general stress response is also strongly induced by heat shock, as we documented previously (22), we plotted the log2 values of the ratios of induction by H2O2 (relative to the untreated control at the same time) versus the log2 values of the ratios of induction by heat stress. Since the kinetic analyses revealed that both responses were strongly and selectively induced at the earliest time point (3 min), we focused on these data for comparison. The resulting graphs revealed two clusters of genes induced by both treatments (Fig. 2, upper right quadrants). One set of genes was strongly induced by either 8 or 58 μM H2O2 but only weakly induced by heat stress. These genes include mrgA, katA, and zosA and define a subset of the PerR regulon (23). A second large set of genes, representing the bulk of the heat shock stimulon, was induced by 58 μM H2O2 (Fig. 2B) but was only weakly affected by 8 μM H2O2 (Fig. 2A). This group includes many known and putative members of the large general stress regulon controlled by σB (21, 30).
Analysis of the kinetics of the transcriptional responses to H2O2 revealed that both the PerR and σB components of the H2O2 stimulon were induced transiently (Fig. 3). Most σB-dependent general stress genes were maximally induced at 3 min following exposure to 58 μM H2O2, and the mRNA levels declined by 10 min following exposure. This was consistent with the transient induction of the σB regulon in response to heat shock and likely reflected the rapid induction of negative regulatory factors, including RsbX (38). The PerR-controlled stress response was also transient, with the maximal mRNA levels occurring at either 3 or 10 min following exposure.
PerR regulon.The role of PerR in the regulation of catalase (katA), mrgA, ahpCF, zosA, and the heme biosynthesis operon has been reported previously (4, 9, 19). To identify additional members of the PerR regulon, we compared the transcriptional profiles of perR mutant and wild-type cells grown to the late logarithmic phase (OD600, ∼1.0) (Fig. 4). Our slides contained DNA probes for 90% (3,703 of ∼4,100) of B. subtilis genes (22), and hybridization signals significantly above the background level were detected for 60 to 70% of these genes. In the comparisons of the perR mutant and the wild type, we calculated expression levels for ∼2,400 genes, and 8% of these levels were significantly altered in the mutant strain (Fig. 4). Expression of ∼75 genes (including known PerR regulon members katA, mrgA, and zosA) was significantly elevated in the perR mutant (Table 1), while expression of another ∼120 genes was decreased.
To identify additional members of the PerR regulon, we compared the effects of 58 μM H2O2 and the perR mutation in a two-dimensional plot (Fig. 5). Known members of the PerR regulon were induced by H2O2 and derepressed by the perR mutation (Fig. 5, upper right quadrant). Remarkably, there was an excellent correlation between induction by 58 μM H2O2 (at 3 min) and the extent of derepression in the perR mutant (correlation coefficient [R2], 0.9). This suggests that PerR repression was completely relieved under these conditions, and the difference in the magnitude of induction may have resulted primarily from differences in the extent to which PerR affected gene expression. Several other genes (including ygbA, yerL, opuBC, and ureB) also mapped to this region of the graph, but these genes were not associated with Per boxes and the effects may have been indirect. Note that the majority of the genes up-regulated in the perR mutant were not peroxide inducible (Fig. 5). Furthermore, the expression of many genes that were induced by 58 μM H2O2 (including many members of the σB regulon, as noted above) was decreased in the perR mutant, and these genes clustered in the lower right quadrant in Fig. 5.
Many of the observed changes in the perR mutant are likely to be indirect effects (Table 1). For example, the perR mutant may be stressed by the production of high levels of catalase, which could deplete heme pools or affect global iron homeostasis. In addition, the lower growth rate of the mutant affects the expression of many metabolic genes. Many of the 75 genes up-regulated in the perR mutant, including genes associated with competence, nutrient uptake, and the CodY regulon, are associated with the transition phase. The perR mutant strain also had significantly decreased expression of many genes, including the σB regulon genes (Fig. 5), several genes of the Fur regulon (ykuNO, yxeB, ywbL, and ywbN) (1), and the PBSX defective prophage (24). While the origins of the latter effect are unclear, PBSX is known to be induced by 100 μM H2O2 (12). Our results suggest that PerR or a PerR-regulated gene may act as a regulator of gene expression for PBSX.
t-buOOH stimulon.We next investigated the transcriptional response elicited by exposure of cells to t-buOOH. Within 3 min of exposure to t-buOOH, more than 100 genes were induced at least threefold compared to the expression in the time-zero sample. At subsequent times many more changes in gene expression became apparent (Fig. 1), but these appeared to be secondary effects and were not analyzed in detail. As a control, we also determined the time course of transcriptional changes elicited by exposure to the corresponding alcohol, t-buOH. A two-dimensional comparison of the responses revealed a remarkable pattern: the vast majority of the genes induced by t-buOOH were induced equally by t-buOH (Fig. 6). Not surprisingly, most of these genes belong to the σB regulon, which is strongly induced by ethanol (31).
There are also several genes that were induced by t-buOOH but not by t-buOH, and these genes are therefore candidates for an organic peroxide stimulon. Most notably, the ohrA gene was strongly and specifically induced by t-buOOH (Fig. 1, second zoom box, and Fig. 6). Expression of ohrA is regulated by the peroxide-sensing transcription factor, OhrR (16, 17). Other genes that were selectively induced by t-buOOH (although no gene was induced as dramatically as ohrA) include the PerR regulon member mrgA, the thioredoxin B gene (trxB), and at least two genes of the ArsR-regulated ars operon (32).
Three peroxide-induced regulons.Overall, our analyses led us to conclude that the peroxide stimulon has three major components: the PerR, σB, and OhrR regulons. These three regulons can be clearly visualized by comparing the transcriptional responses (after 3 min) to 58 μM H2O2 with the responses elicited by 100 μM t-buOOH (Fig. 7). Three distinct sets of genes clearly emerged, as follows: the OhrR-regulated gene, ohrA, was strongly induced by t-buOOH but not by H2O2; the σB regulon was induced by t-buOOH more than it was induced by H2O2; and the PerR-regulated genes were induced by H2O2 more than they were induced by t-buOOH. We summarized the selectivity of these transcriptional responses by comparing the efficacies of various inducers for representative members of each regulon (Fig. 8).
Here we provide a global overview of the major transcriptional changes elicited by various peroxidative stress conditions. Our findings indicate that PerR is the major regulator of genes induced by low levels of H2O2, while OhrR regulates the single gene that is most rapidly and selectively induced by the model organic peroxide t-buOOH. However, the largest single group of genes induced by these treatments is the σB-dependent general stress regulon (30). Indeed, knowledge about the general stress response regulon, as deduced both from previous studies of the heat shock stimulon (22) and from extensive characterization of the σB regulon (21, 30), provided important background for our analysis.
Our work also illustrates the value of monitoring the kinetics of global changes in gene expression following application of a stress. Many of the most dramatic transcriptional effects were maximal within 3 min of application of the stress conditions, and there was an often rapid return to lower expression levels within 10 to 20 min. The transient nature of the σB-dependent general stress response is well known, but transient induction of the PerR and OhrR regulons has not been well documented previously. Thus, studies based on a single time point may miss major parts of the transcriptional response.
Our results emphasize the importance of control experiments for interpreting transcriptional profiles. We found, for example, that the vast majority of the transcriptional changes noted 40 min after H2O2 treatment were also observed in mock (H2O)-treated samples and were due to a growth phase transition rather than any effect of the peroxide treatment. Similarly, the comparison of the t-buOOH and t-buOH stimulons (Fig. 6) highlighted the need to use appropriate controls when the effects of particular classes of compounds are investigated. Indeed, we cannot exclude the possibility that t-buOOH is an inducer of the σB regulon because it is reduced in vivo to t-buOH. However, the rapid kinetics of these transcriptional responses make it seem unlikely that reduction to the alcohol is necessary for induction.
While transcriptional profiling is an extremely powerful tool for monitoring changes in gene expression after a change in culture conditions, the comparison of mutant and wild-type strains presented additional challenges. In this case, the perR mutant and wild-type strains differed significantly in growth rate and physiological state. Despite these differences, the perR mutant clearly revealed derepression of known PerR-repressed target genes. Remarkably, the extent of derepression in the samples corresponded very well with the fold induction 3 min after treatment with H2O2 (Fig. 5). This correlation suggests that the magnitude of peroxide induction of different genes reflects the extent to which PerR represses gene expression. As noted elsewhere, the fur gene is unusual among PerR-repressed genes in that it is not peroxide inducible under these or any other conditions tested (18). While not apparent from the transcriptional profiling data, PerR does mediate an approximately fourfold repression of fur in response to Mn(II) (18).
It is instructive to compare the B. subtilis and E. coli peroxide stress responses. In E. coli, transcriptional profiling was performed with cells treated with much higher levels of H2O2 (1 mM), in part because lower levels led to only transient changes in gene expression (39). Under these conditions 140 genes were induced approximately fourfold in the wild-type strain, and an even greater number of genes were induced in an oxyR mutant strain. The OxyR regulon includes genes with protective and detoxification functions, such as katG (hydroperoxidase I), ahpCF (alkyl hydroperoxide reductase), and dps (DNA-binding protein). In addition, OxyR activates transcription of genes that maintain intracellular thiols in their reduced states, including gorA (gluthathione reductase), grxA (glutaredoxin), and trxA (thioredoxin 2). In aerobically growing E. coli the concentration of intracellular H2O2 is maintained at levels near 20 nM by the potent peroxidase activity of Ahp (10, 11). Catalase peroxidase appears to be most important in detoxifying higher levels of peroxides and has the added advantage of being active even in energy-depleted cells that may lack sufficient reducing capacity to maintain optimal Ahp activity. Ahp itself was originally characterized as the major resistance factor protecting cells against alkyl peroxides. However, Ahp is also active with H2O2, and this may be the physiologically relevant substrate (10).
The PerR regulon differs from the regulon controlled by OxyR in several respects. PerR does not appear to control functions that might be involved in maintaining intracellular thiols in a reduced state. Although B. subtilis encodes several predicted peroxiredoxins, including a thiol-dependent peroxidase (tpx) and the product of ygaF (the gene immediately upstream of perR), only the ahpCF genes are members of the peroxide stimulon characterized in this study. In future work, it will be important to define the roles of catalase, Ahp, peroxiredoxins, and the organic hydroperoxide resistance proteins in B. subtilis. While all of these enzymes may act to detoxify reactive oxygen species in the cell, genetic studies indicate that they are not redundant.
This work was supported by grant MCB-9983656 from the National Science Foundation to J.D.H.
- Received 5 August 2002.
- Accepted 30 September 2002.
- Copyright © 2003 American Society for Microbiology


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15 Useful Gadgets For Living Anywhere - Part 2.


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You are currently browsing the tag archive for the 'Online crop' tag.
So over at Shimelle, there is a summer online crop going on – full of hourly scrap and photo challenges. Because it's winter and raining here, and I am having a girly weekend, I spread out my stash and got into it this morning. (Those guys in the Northern Hemi have been scrapping while I have been sleeping, so I am playing catch up!).
In this post I am going to add my layouts from the scrap challenges, and I will post another for the photo challenges.
This is what my lounge looked like when I started…you don't even wanna know what kind of state it's in now!
Challenge number one was to scrap a page using red, aqua and white. It was a bit hard to get into the creative swing of things, but once I got going, I was away. Here is the layout I created – "you can't beat Wellington on a good day":
The second challenge, which I found much harder, was to scrapbook using a sketch provided. I'm not much of a person for a sketch, I tend to freestyle it depending on what embellishments I want to include, or how I feel the photos work best. So even though I had the perfect picture to use – the simplicity of this sketch was tough! Here is my layout, it's of a fluke photo we captured (yay autotimer) when we visited a remote beach village of Koitiata. I love how the sunset is bursting out of our clasped hands.
The third challenge involves printing out an alphabet – so will have to wait until I get access to a printer at work on Monday.
The fourth challenge was to scrap with circles. My first reaction was "oh guts, I don't have anything with circles on it" – but then I started sorting through my stash of papers. Ha! Turns out I had more than 10 sheets with dots or circles on them! Amazing what you find. I decided to do a double page (I was really getting into it now, someone hold me back!) about a concert that Jase and I went to last summer. The best thing? Being able to FINALLY use these awesome Creative Imaginations Rock Star stickers – which Jase gave me for my birthday a whopping TWO years ago. Yay! Stash busting at its best.
Challenge number five was to scrapbook with a black and white photo. I didn't want to leave the house to print off a new black & white shot, so I scrounged around and found this pic from our walk to Red Rocks. It's black and white, but the rocks are red – it was the closest I had!
The final challenge for day one was to scrap a page with more than four patterned papers. Well – I felt like this challenge threw me into real chaos! However I think I finally managed to pop a variety of patterns together in a way that doesn't clash…well, nottoo bad I hope?! The story behind 'Breaking the Rules' is my mum's joke – she always told me that I was to "never come home with a tattoo, never come home pregnant, and to never come home on the back of a motorbike". Strangely enough, I listened…until last June when I hopped on the back of Jase's dirtbike and took a ride down Waitarere Beach! I think at this age mum might just let me off?!
Ok, that's it for tonight. I have a mighty sore back from sitting on the floor all afternoon – and somehow its 11.25pm! Bed time, and more layouts to come tomorrow hopefully!


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Be Happy YOU Don't Live In A Third World Country!
I just got this from my friend Angela…this is so true! We all must be grateful for all we have been given & use it wisely & judiciously. As the Bible says:
To Whom much is given, much is expected.
~~Luke 12:48
Just be happy YOU don't live in a third world country!
No matter what your political convictions are, this is an eye opener….What a thankless people we are!!!
David Letterman on President Bush. (Surprising; it's the David we don't often see….)
{This essay was inaccurately attributed to both David Letterman and Jay Leno. It was actually written by Craig R. Smith in 2006.}
The other day I was reading Newsweek magazine and came across some poll data I found rather hard to believe. It must be true given the source, right? The same magazine that employs Michael (Qurans in the toilets at Gitmo) Isikoff. Here I promised myself this week I would be nice and I start off in this way. Oh what a mean man I am.
The Newsweek poll alleges that 67 percent of Americans are unhappy with the direction the country is headed and 69 percent of the country is unhappy with the performance of the president. In essence 2/3s of the citizenry just ain't happy and want a change.
So being the knuckle dragger I am, I starting thinking, "What we are so unhappy about?"
Is it that we have electricity and running water 24 hours a day, 7 days a week? Is our unhappiness the result of having air conditioning in the summer and heating in the winter? Could it be that 95.4 percent of these unhappy folks have a job? Maybe it is the ability to walk into a grocery store at any time and see more food in moments than Darfur has seen in the last year?
Maybe it is the ability to drive from the Pacific Ocean to the Atlantic Ocean without having to present identification papers as we move through each state? Or possibly the hundreds of clean and safe motels we would find along the way that can provide temporary shelter? I guess having thousands of restaurants with varying cuisine from around the world is just not good enough. Or could it be that when we wreck our car, emergency workers show up and provide services to help all involved. Whether you are rich or poor they treat your wounds and even, if necessary, send a helicopter to take you to the hospital.
Perhaps you are one of the 70 percent of Americans who own a home, you may be upset with knowing that in the unfortunate case of having a fire, a group of trained firefighters will appear in moments and use top notch equipment to extinguish the flames thus saving you, your family and your belongings. Or if, while at home watching one of your many flat screen TVs, a burglar or prowler intrudes; an officer equipped with a gun and a bullet-proof vest will come to defend you and your family against attack or loss. This all in the backdrop of a neighborhood free of bombs or militias raping and pillaging the residents. Neighborhoods where 90 percent of teenagers own cell phones and computers.
How about the complete religious, social and political freedoms we enjoy that are the envy of everyone in the world? Maybe that is what has 67 percent of you folks unhappy.
Fact is, we are the largest group of ungrateful, spoiled brats the world has ever seen. No wonder the world loves the U.S. yet has a great disdain for its citizens. They see us for what we are. The most blessed people in the world who do nothing but complain about what we don't have and what we hate about the country instead of thanking the good Lord we live here.
I know, I know. What about the president who took us into war and has no plan to get us out? The president who has a measly 31 percent approval rating? Is this the same president who guided the nation in the dark days after 9/11? The president that cut taxes to bring an economy out of recession? Could this be the same guy who has been called every name in the book for succeeding in keeping all the spoiled brats safe from terrorist attacks? The commander in chief of an all-volunteer army that is out there defending you and me?
Make no mistake about it. The troops in Iraq and Afghanistan have volunteered to serve, and in many cases have died for your freedom. There is currently no draft in this country. They didn't have to go. They are able to refuse to go and end up with either a "general" discharge, an "other than honorable" discharge or, worst case scenario, a "dishonorable" discharge after a few days in the brig.
So why then the flat out discontentment in the minds of 69 percent of Americans? Say what you want but I blame it on the media. If it bleeds it leads and they specialize in bad news. Everybody will watch a car crash with blood and guts. How many will watch kids selling lemonade at the corner? The media knows this and media outlets are for-profit corporations. They offer what sells. Just ask why they are going to allow a murderer like O.J. Simpson to write a book and do a TV special about how he didn't kill his wife but if he did … insane!
Stop buying the negative venom you are fed everyday by the media. Shut off the TV, burn Newsweek, and use the New York Times for the bottom of your bird cage. Then start being grateful for all we have as a country. There is exponentially more good than bad.
I close with one of my favorite quotes from B.C. Forbes in 1953:
"What have Americans to be thankful for? More than any other people on the earth, we enjoy complete religious freedom, political freedom, social freedom. Our liberties are sacredly safeguarded by the Constitution of the United States, 'the most wonderful work ever struck off at a given time by the brain and purpose of man.' Yes, we Americans of today have been bequeathed a noble heritage. Let us pray that we may hand it down unsullied to our children and theirs."
I suggest this Thanksgiving we sit back and count our blessings for all we have. If we don't, what we have will be taken away. Then we will have to explain to future generations why we squandered such blessing and abundance. If we are not careful this generation will be known as the "greediest and most ungrateful generation." A far cry from the proud Americans of the "greatest generation" who left us an untarnished legacy.
As if something as thought provoking as this could have come from either Leno or Letterman! Although it was nice for a while to think That David grew a heart and penned it. In any case, this is true: George W. Bush has kept us safe since 9/11 and most of us are an ungrateful lot! Being thankful for what you do have should be a law. We're all too busy trying to keep up with The Jones' to see what's right in front of us. It's a very sad commentary on our society. Don't you think we should do something to change it?
Category: Politics
About the Author (Author Profile)I'm Shan and I 'm the creator of The Asylum and a magnet for The Free Range Stupid™. I'm a little nutty, a lot sarcastic and pretty damn smart. I am also a graphic designer, blog coder, virtual assistant, free lance writer and can whip you up a killer resume, media kit or press release that would make others green with envy. Go to Skewed Design Studios to check out my services. You won't be disappointed.
Comments (6)
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Wonderful post. I actually read the news each and every morning with my coffee to remind myself that no matter what's going on in my life – I have it pretty good. I could, you know, be in Zimbabwe, Darfur…Thanks for putting this out there.
Wonderful post. I actually read the news each and every morning with my coffee to remind myself that no matter what's going on in my life – I have it pretty good. I could, you know, be in Zimbabwe, Darfur…
Thanks for putting this out there.
Oh AMEN! I actually stopped reading someones blog because she said "I am ashamed to be an American." This is the best country in the world and maybe if more of us acted like it – things would be looking better right now.
I actually feel sorry for "W" at this point, ending his presidency on this note. Many of the good things he has tried to do have been blocked by a democratic congress, and the man has kept this country safe for 7 years.
I remember him on September 12th, standing amongst the rubble at ground zero and I was proud that he was leading us at that moment. I am sorry people can only see the bad and won't even look at the good he has done. It certainly is an unpopular viewpoint these days.
And even if you don't like the president – you get to express that out loud – because this is the best country in the world.
hey–i finally got back and read your comment on my blog. i don't have your email or else i'd do it that way…but thank you.
what you said helped me A LOT.
Hi Babyrockasmama,
I have responded to your comment in HTML code displayed in scroll box
Hi Babyrockasmama,
I have responded to your comment at Blogger Tips and Tricks is now 4 column


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Australia's internationally renowned luxury boat builder, Riviera, in conjunction with US east coast representative Grande Yachts International, will proudly showcase the sophisticated 3600 Sport Yacht at the Bay Bridge Boat Show in Stevensville, Maryland, opening on April 27.
Grande Yachts International dealer principal Bryan Braley said he and his team are proud to display this luxurious model to US east coast motor yachting enthusiasts at the Bay Bridge Marina.
"Riviera's 3600 Sport Yacht offers a welcoming and superbly equipped cockpit with a luxurious and comfortable interior to create an alfresco layout that is the hallmark of Riviera motor yachts.
"Riviera and Grande Yachts International are fully committed to fulfilling the lifestyle dreams of America's discerning motor yacht owners," said Mr Braley.
Riviera 3600 Sport Yacht on display at the Bay Bridge Boat Show
The 3600 Sport Yacht epitomizes easy living and an idyllic environment to share leisure time with family and friends.
Riviera's International Sales Director, Chris McCafferty, said: "She has clean lines, an open and bright interior, proven technology and a real emphasis on relaxed single-level entertaining. This is perfectly blended with Riviera's legendary sea-keeping capabilities and spirited performance."
The 3600 Sport Yacht features Riviera's hallmark rear awning window between the cockpit and the salon and aft galley to create a welcoming indoor-outdoor experience.
"Accommodation for the Riviera 3600 consists of a master stateroom forward with a large island bed and an aft full-beam guest stateroom with a double and single bed, catering for up to five adults on the lower deck," says Mr McCafferty.
"The 3600 Sport Yacht features easy joystick manoeuvrability which enables the skipper to simply push and twist the joystick in the required direction to make the yacht respond immediately, pivoting in place or tracking sideways into a berth that's just long enough to fit the yacht."
Power comes from Volvo Penta's D4-IPS400 system with twin D4 turbo diesel engines (2 x 221kW/300hp) and IPS10 pod-drive units. With Riviera, all Volvo Penta systems, drives and electronics are covered by an industry-leading five-year limited warranty to complement Riviera's five-year structural limited warranty.
Where to find Riviera at the Bay Bridge Boat Show
Grande Yachts International Dealer Principal Bryan Braley said: "We are looking forward to welcoming Riviera owners and visitors to inspect the luxurious Riviera 3600 Sport Yacht at our stunning on-water showcase at the Bay Bridge Marina on Marina Club Road in Stevensville, Maryland, from April 27 to 29."


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Stink is both the alter-ego of artist Evan Rossell, and Evan's flagship creation. The ring-lead of the Hairy Fools, Evan's illustrated crew of troublemakers, Stink's appeared on everything from skateboards to paintings to the broken chair outside the CONVICTS office.
Despite the Southern California, pastel hues that Evan works, a hard-to-place spookiness lurks in his creations. CONVICTS caught up with Evan before his recent trip to New York. We got his word on street art, the importance of travel, and of course, the Hairy Fools' origin story.
Hey mate. To start can you tell us where you're from?
I'm from California, Southern California. Right now I live in Newport.
What was your childhood like?
Childhood was like skateboarding, riding my bike. Very outdoorsy.
Who did you hang out with growing up?
Growing up my mom and dad just took me and my brother to skateparks and the BMX track. So it was like meeting different kids at the skateparks.
When did you get into art?
I didn't get into art 'til like high school…and that's when I learned how to surf.
Where else have you lived?
I used to live in Colorado. We would go mountain biking and whitewater rafting and rock climbing, that was like a normal weekly routine. When we moved here I wanted to learn how to surf and kids at school were doing art so I wanted to start doing some art. I was super inspired by skateboarding and surfing so I started making my own little doodles.
Can you remember those first little doodles? What were they about?
They were just like a little cartoon. Like a half-guy with a lil' smiley face. It was super basic but at the time I was so stoked just to have a little guy doodled on my binder and stuff.
What was the progression? As in, how did you get from doodling on homework to where you are now?
I had the raddest art teacher my senior year and he opened up my mind to screen printing. Turning a doodle into a t-shirt or a skateboard deck or a sculpture. It was rad to see your art progress.
Talk about the Hairy Fools.
Ahh, the Hairy Fools. So I'd do a bunch of little hairy colorful monsters and me, Stink. They're the crew I never had. The surf crew, skate crew, graffiti crew that I wish I had…so these are just a bunch of little Hairy Fools. Then from there I was like 'Everybody can be a part of it.' Everybody's colorful and hairy in their own way, you know?
Do you try to keep that childlike sense of wonder that kids have when they start to draw and name their crews? Or are you thinking specifically and down the line?
Specifically. I think of it as making something that everyone can be a part of. So it's Stink and Hairy Fools. Stink is the main Hairy Fool.
Is that how you see graffiti? Or street art? As something that everyone can be a part of?
For sure.
Where do you think street art is now? In relation to where it came from and what it's doing?
You see it a lot more now and it's a lot more open. Communities are more open to it. In the 80's it was more to get your name on a wall and be seen by hundreds of people a day, whereas now you can actually make a living doing murals all over the world and create a name for yourself. If nobody saw my art I would still make it because it's just what I like to do, but it's nice to get hired by cities and countries to go paint and experience things that you probably wouldn't have experienced. It's super positive and fun.
How does experiencing new places and things influence your work?
Well, looking up to an artist for so long then eventually meeting them and being able to paint a mural with them in their country is so awesome. It's just rad to see their culture and how they live and it makes me wanna put that into my art. So like traveling to places like Brazil and South America and got me started doing all these little houses. Just because when you're going through the city you see a lot of these houses stacked on top of each other so I started stacking houses around my characters. Bringing those different cultures that into my art is very special. I don't say it's inspired by this, I just put it out there because it's special to me.
But you said you've never been to New York City before?
What do you think it's gonna be like when you get there?
A playground. I've heard you don't drive a car, you take people movers everywhere, and there's just art all over the street. I've see pictures from back in the day to now, and it just looks like a big playground of fun.
Does art run in your family?
It does. My mom is a really good artist. Growing up she was always doing crafts. Very DIY. My brother would say he wanted a mural on his wall and she would paint a mural on his wall. Looking over his portfolio inspires me to keep making work and using my name, and hopefully one day put him on a platform because it's just insane to see all his work. It's almost like he wasn't noticed for it but it's super legendary and makes me wanna live the lifestyle and keep doing my art for the family.
Where was your grandpa from?
He's from Peru, actually. My grandpa and his four children from Peru were just like "We're gonna go live in America." That's pretty special.
What role does California play in your life?
In my life? Everything.
What do skating and surfing do for you? Is it therapeutic?
Yeah they're totally therapeutic.
Do you ever have moments where you're not inspired? Where you need to re-ignite the artistic spark?
I change up by doing things outside of the norm. What's really inspired within the last year has been creating clothes for myself and painting on different mediums, it just helps me kinda think more outside the box and generate new ideas. Travelling also helps. And surrounding myself around different groups of people. I get inspired by people.
Alright, last question. Any words of advice for the aspiring kids out there?
The message I try to promote is just to be creative. You can do it yourself, you don't gotta go hire somebody to do it.
Thanks, man. Best of luck with everything.


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Know What You Want
Know what you want to get out of your treatment, whether it's clearer skin or just an hour of relaxation and pampering -- and don't be afraid to communicate those wants to your esthetician. "I think it's absolutely okay to let their esthetician know what their expectations are because that allows the esthetician to meet those expectations," says Katie Hamilton, Program Coordinator and Esthetician with Associated Skincare Professionals (ASCP). That includes telling your esthetician about any personal preferences; for example, that you like a particularly gentle touch, or that you'd rather she not talk during the treatment.
Let your esthetician know about any problems or issues you're having with your skin. "Everybody knows if they have sensitive skin that reacts to new products, or whether they're prone to new breakouts, or if their skin is really tough," says Tami Meraglia, M.D., Medical Director and Owner of Vitality Medispa and Wellness Center in Seattle, Wash. If you don't tell your esthetician about these issues ahead of time, you could walk out with a bad reaction from the products used.
Be clear on how much you want to spend. Skin care service prices can vary widely depending on where you live (big cities like Los Angeles and New York are typically more expensive than small towns) and what type of facility you visit (spas and hotels tend to be pricier than salons). Find out what the facility charges before you go or you might end up with a severe case of sticker shock.


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15/04/2019 - 10:25:00Back to Banks Ireland Home
Greater transparency, treating customers with fairness and maintaining the highest ethical standards will be the focus of a new board tasked with overseeing a change in banking culture.
The Irish Banking Culture Board has been established to ensure a change within the banking system for staff, customers and stakeholders.
One of the key findings of a survey from customers is that there is not enough transparency and there should be 'less jargon' in banking documents.
Chairperson of the IBCB is former Court of Appeal judge John Hedigan, who says it is time the banks gave something back to the public who helped bail them out.
"To whom much is given, much is asked," said Mr Hedigan.
"And the Irish people gave to the banks their salvation at huge cost to themselves.
"And they deserve to have something very significant given back in return. And this, I hope, is it."


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Offers Virtual Services
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Sheena R. said "We asked these guys to play at our wedding for 2 hours. Despite the heavy traffic they were at the venue on time. Because it was first dance we spoke…"


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Lumex is announcing a new line of surface-mount technology (SMT) LEDs which provides the features and capabilities of conventional through-hole T-5mm epoxy lens LEDs. These LEDs have an off-the-board height of 11mm (0.43 inches) and occupy a circuit board footprint which is 6mm x 6mm (0.24 inches x 0.24 inches) They are available on standard 24mm tape for automatic insertion.
Lumex OctoLEDsTM patent-pending devices are the result of a manufacturing breakthrough, which makes Lumex the first to offer this class of surface-mount LEDs. Most assemblies are now moving toward SMT technology. This design would be a direct replacement to standard 5mm through-hole LEDs. Lumex OctoLEDsTM are ideal for any design that is transitioning from through-hole to SMT technology, without sacrificing any of the intensity.
Lumex OctoLEDsTM (Series Number SML-H1505xxx-TR) can be used anywhere the convenience of surface mounting is required, with the high brightness, color and viewing angle choices of standard T-5mm LEDs. Such applications include front panel, PCB status or fault indicators, as well as light bars or dot matrix arrays where each LED needs to be individually-addressable.
Not only do these new LEDs provide the mounting and assembly convenience of surface-mount technology, they also deliver a high brightness level of up to 7,000mcd (dependent on color) It is the first time that this degree of brightness has ever been available from any conventional SMT LED running at the common If of 20mA.
Lumex OctoLEDsTM are available in the full range of colors including red, yellow, green, blue, or white. Bi-color as well as RGB versions are also offered. The available variety of lens finishes open the door to different viewing angle options.
These new units can withstand soldering temperatures of up to 10 seconds at 255º C. A gradual temperature rise of 2º per second reaching 150º C is recommended.
Lumex OctoLEDsTM are RoHS compliant.
# # #


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What do for can improve?
- Take the medicines how were recipients: Surely it will be a treatment with analgesics or anti-inflammatory classical or incorporate medicines modulators of the pain: anti depressives and anticonvulsants mainly. The objective is to improve the dream, the fatigue, the depression, them spasms muscular and the pain. In the gravest cases, can combine varies of these drugs, under prescription medical.
- Practise good routines to sleep with the purpose to improve the quality of the dream.
- Do exercise: The experts recommend to realise aerobic physical exercise of progressive shape, gradual and constant (swim, walk…).
- Diet balanced: Mediterranean Diet
- Avoid the caffeine.
- Leave the stress side by side
- Test with treatments of acupressure and acupuncture.
- The doctor can remit it to a clinical of the pain if him affective is grave.
Psychological therapies:
This aspect of the treatment is fundamental to teach to the patient to face of correct shape it fibromyalgia.
The psychological therapies are used to to improve some symptoms how:
- The pain
- The depression
- The autonomy
- The living quality of the patient.
And also it helps to:
- Handle negative thoughts.
- Bring a newspaper of his pain and symptoms
- Recognise what worsens the symptoms.
- Look for pleasant activities.
- Establish limits.
- Establish contact with groups of support or associations of fibromyalgia also can be very useful.
Another type of therapies: Disciplines of the health that offers a therapeutic alternative no pharmacologic to diagnose, prevent and treat symptoms of multiple ailments, so much acute as a chronic, by means of the therapeutic exercise, heat, cold, light, water or manual technicians between them the electricity.
- Massage: Useful to calm and increase the circulation of the blood to the taut and painful muscles.
- Relief miofascial: Manipulation very soft of massage designed to relieve the restrictions and him estretor of the fabrics connective. When it practises properly, many times achieves to diminish the pulled that exert the fabrics connective on the bones, allowing that they relax and extend the fibres of the muscles and that they expand the organs.
- Aerobic exercises: To avoid the muscular atrophy
- Osteopathy
- Exercises of stretch
Living quality
In the actuality there is one big numeral of people with fibromyalgia that they realise a normal life. Nevertheless, if the illness it takes to diagnose or does not realise the suitable intervention, the living quality of the patient goes diminishing slowly.
The importance of this pathology lies that it affects to all the fields of the life of the patient and this can reach develop a disabled that even interfere in his labour field. As several studies, between the 15 and the 20 and until the 50 percent of the patients have to leave his work.
The diagnostic is clinical and explores to the patient by means of analysis of blood, X-rays and other complementary tests that allow the exclusion of illnesses with alike symptoms. The analysis helps to discard other illnesses how the lupus, the hypothyroidism or him arthritis rheumatoid and can confirm that it is fibromyalgia when detect a group of 18 points, denominated painful points to the pressure that are used to to situate in several muscular areas of the body how the neck, elbow, knees and pelvises.
Which are the causes of it fibromyalgia?
The causes of it fibromyalgia are unknown. Some factors can be related with this disorder.
It fibromyalgia has related with:
- Events stressfulor traumatic, as a accidents automobiles.
- Recurrent injuries.
- Unrests or ailments.
- Some illnesses.
It fibromyalgia diagnoses more frequently in individuals that suffer some illnesses. These illnesses can share clinical demonstrations with the FM:
- Arthritis rheumatoid
- Lupus erythematosus systemic (comunament called lupus)
- Spondylitis ankylosing (arthritis Spinal).
- Spondyloarthropathies
- Multiple sclerosis
- Hypothyroidism
- Neuropathies Peripheral
- Mechanical structural alterations or degeneratives of the Neuropathies
- Myopathy (metabolic or inflammatory)
- Polymyalgia rheumatic
- Depressive disorder Greater
- The women that have to a familiar with fibromyalgia have greater predisposition to suffer of fibromyalgia also.
Some scientists think that that the origin of it fibromyalgia can be genetic. The genes can do that a person have a strong reaction of pain in front of things that other people do not consider painful.


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The search found 11 results in 0.009 seconds.
Actresses need to be pickier than men about with whom they work if they want to survive in the movie industry, suggests a new study.
"My research indicates that women in the film industry suffer a lack of access to future career opportunities when they tend to work with people who have collaborated frequently in the past," said Mark Lutter, lead author of the study and head of the "Transnational Diffusion of Innovation" Research Group at the Max Planck Institute for the Study of Societies (MPIfG) in Germany.
Heterosexual couples that split childcare duties have higher quality relationships and sex lives than those who don't, according to new research that will be presented at the 110th Annual Meeting of the American Sociological Association (ASA).
The majority of young women and men today would prefer an egalitarian relationship in which work and family responsibilities are shared equally between partners if that possibility were available to them, according to a new study from the University of Texas at Austin and the University of California-Santa Barbara.
People whose sexual identities changed toward same-sex attraction in early adulthood reported more symptoms of depression in a nationwide survey than those whose sexual orientations did not change or changed in the opposite direction, according to a new study by a University of Illinois at Chicago (UIC) sociologist.
Social scientists have long argued documentary films are powerful tools for social change.
But a University of Iowa (UI) sociologist and his co-researchers are the first to use the Internet and social media to systematically show how a documentary film reshaped public perception and ultimately led to municipal bans on hydraulic fracking.
The number of children in foster care across the country is driven not solely by child abuse and neglect, but by states' varying politics and approaches to social problems, a new University of Washington (UW) study finds.
States with more punitive criminal justice systems tend to remove children from their homes far more frequently than those with generous welfare programs — meaning that two states with similar rates of child abuse and neglect could have very different rates of foster care entry.
Squatters who illegally occupy vacant homes or buildings are not always contributing to apathy or social disorder, says a new University of Michigan study that was presented at the 111th Annual Meeting of the American Sociological Association (ASA).
It can actually be a good situation for a neighborhood to have these individuals move into abandoned homes, lessening the chance of them becoming sites for drug users or burned by arsonists, the study indicates.
A tiny hair barrette and an anguished moment marked the turning point for one mother in coming to fully accept that her child, who was born a boy, was a transgender girl.
Quinn had expressed a preference for girls' clothing and accessories at a young age, but Jessica and her husband, Steve, would not allow her to wear them outside their home.
One day, picking her up from school, Jessica watched Quinn quickly remove a barrette from her hair and slip it into her pocket, ashamed that her mother might have seen.
Community characteristics play an important role in perpetuating teen suicide clusters and thwarting prevention efforts, according to a new study by sociologists at the University of Chicago (UChicago) and University of Memphis who examined clusters in a single town.
Mothers who leave work to raise children often sacrifice more than the pay for their time off; when they come back their wages reflect lost raises, according to a new study by Paula England, Professor of Sociology at New York University.


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Wednesday, September 02, 2009
PMS Sneaks-Day 2: Introducing...the Moody Girls!
Now I must add a brief disclaimer--I (like several of the other DT members) were admittedly intimidated by the Moody Girls when we first saw them. It was with trepidation that I chose one to demo...but let me tell you something...once I started coloring, I got into it more and more and MORE! Now I can't even believe I thought this was going to be something hard! I had sooo much fun coloring my image and now I am considering some of the other Moody Girls for my collection.
As you might have guessed, my card for you today features one of the Moody Girls--Moody in Pumpkin Patch. I love all things fall, so her pumpkins appealed to me. Her outfit was VERY cool to color with the little bits of detail. I added chalk to the ground and sky portion of the image and went over her coat buttons with a Gold Spicca.
My layout for this card is this week's Inspirational Card Sketches #32 . I really liked the layout this sketch challenge provided and it was fun to play with. The top and bottom strips of paper were run through my Cuttlebug with the leafy branch embossing folder--it gives them some leaf texture even if you can't really see it in this picture. The bottom strip also has the words "Give Thanks"--a rub-on that just didn't show up as bright like I thought it would. I think I'm going to go over that with something to brighten up the words. The only other thing that bugs me about this card (and really it's minor) is how much brighter the image appears compared to the rest of the card. But I think a lot of that is lighting...because in real life, it's not that much of a contrast.
Card Details
Stamps: Moody in Pumpkin Patch--available Sept 7th (PMS)
Coloring: SU!, BIC, Prismacolor and Copic (E00) markers
Paper: CS-Rust, Mocha, Hunter Green (Paper Studio), White (GP); DP-"Autumn Leaves" (Paper Studio) and variegated brown from stash
Accessories & Tools: twine, dimensionals, Stickles (Stardust), gold spicca pen, white gel pen, brown metallic brads, "give thanks" rub-on, Cuttlebug w/"Leafy Branch" EF
Now you need to go check out the other three fabulous Moody Girls! You can find them over on the Paper Makeup Stamps blog post for today! Let me know what you think about them!
See you tomorrow, for your first peak at some new PMS images from one of the most FAVORITE categories--FAIRIES!
Don't forget to check out the Freebie Friday Giveaway on the Paper Makeup Stamps blog! It's a super cute piece of Word Art and you only have until September 3rd to Download it! You can find it HERE.
10 Words of Love:
- Heather D. ~ Fresh Brewed Designs said...
Miss Bee!!! I love your card!!! GORGEOUS!!! What a fabulous image and the papers are perfect! So glad you were able to join in at ICS this week. :) <3 Heather
- September 2, 2009 at 11:08 AM
- Christi Flores said...
Ooohhh I love this!!!!
- September 2, 2009 at 11:43 AM
- Tricia said...
the colors are so rich and nice ! love that image! thanks for joining us at ICS this week!
- September 2, 2009 at 12:58 PM
- Tina said...
Absolute perfection Lisa!
- September 2, 2009 at 1:05 PM
- Kim said...
Good gosh, Lisa, she's amazing!
- September 2, 2009 at 7:47 PM
- Fink said...
Just gorgeous!!!! I love it!
- September 3, 2009 at 12:33 AM
- Liz Weber said...
Hi Lisa, your card is so beautiful - I just love the attention to detail and I am so glad you could join us at ICS this week!
- September 3, 2009 at 3:05 AM
- Dawn♥ said...
WOW Lisa it's absolutely breathtaking. Love the image and her coloring. Love the strips of embossed papers. Thanks for playing along! :)
Owner of ICS
- September 3, 2009 at 10:08 AM
Your card is` gorgeous` Thanks for joining in with the ICS this week:)x
- September 3, 2009 at 5:15 PM
- wendipooh13 said...
what a gorgeous card and love all the details that you used!!! great work.. way to go with the IC sketch this week..
- September 7, 2009 at 1:36 AM
Post a Comment
Thank you so much for stopping by my blog today! I love getting comment love, so feel free to make my day! :)
FTC Declaration
*Paper Makeup Stamps
*Pink Cat Studio
*DeeDee's Digis
*MelJen Designs
*Tickled Pink
*Heart Song Stamps
*Lindsay's Scrapbook Shop
*Tiddly Inks
*Wags N Whiskers
* Paper Shelter
*My Favorite Things
*Saturated Canary
*Bugaboo Stamps


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Cost/Share Programs
Tree and Brush Removal Program - The Crawford County Board of Supervisors has adopted a policy that addresses clearing of trees and brush along the County R.O.W. This policy works with the landowner and a private contractor. When all required stipulations are met, including proof of Liability Insurance and an approved permit signed by the County Engineer, then work may be performed on a cost/share basis. Payment to the Contractor for clearing and grubbing within the right-of-way shall be made at the rate of $3.00 per clearing and grubbing unit on areas adjacent to gravel roads, and $1.00 per clearing and grubbing unit on areas adjacent to dirt roads. A copy of this policy can be found on the Permits and Policies Page.


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For TFP or Paid Shoots
- Before any shooting takes place, I will discuss with the model, or their agent, the global idea of the shoot. I will explain how and what we will be shooting and will go over any wardrobe and other items used for the shoot.
- If I provide a location for the shoot (studio, hotel, apartment, etc.) I will do my best to make sure it is safe and sufficiently private for the purposes of the shoot.
- During the shoot I will make sure there are sufficient short breaks, for shoots that last longer than 2 hours, a longer meal break can be scheduled in. Both parties are responsible for indicating when breaks are needed.
- The model's safety always comes first. I will bring an assistant to outdoor shoots if we feel this is needed (i.e. shooting in a busy location, or to be closer to the model than I will/can be). Also any equipment I use should not pose a safety hazard towards the model, or the location we are shooting in.
- The model should be aware that they can end the shoot at any point for any reason, and we can re-evaluate our agreement.
- The modelrelease will be presented to the model, or agent, before the shoot but will have to be signed AFTER the shoot. The reason for this is the following wording in the contract : ' The model affirms that all poses, positions, and situations enacted were done with models full consent'.
- A model's ID will be required for adult themed shoots, to be shown before the shoot starts.
- Any agreed payment will be made after the shoot concludes and the modelrelease has been signed by the model.
- If the model is showing signs of being under the influence of alcohol, narcotics, or other judgement impairing substances, at any point before, or during the shoot, I will end the shoot immediately.
- If the model is not able to clearly consent to the shoot, I will end the shoot immediately.
- If the agent representing the model, guardian, or chaperone, involves themselves with the shoot i.e. directing the model, pushing the model to do something they don't want, or anything that would influence the outcome of the shoot, the shoot will end immediately.
- If the model is showing signs of being under the influence of alcohol, narcotics, or other judgement impairing substances, at any point before, or during the shoot, I will end the shoot immediately.
- If I end the shoot because of any of the reasons under point 9, no payment will be made.
- Removal of material on request of the model will always be taken into consideration and honoured in case of TFP shoots, for paid shoots a reasonable fee will be required to offset the loss of potential income, or reimbursement of the fee paid to me.


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The Hull Adult Education Centre offers full-time academic courses to students.
Full-time attendance is required to remain in the Centre, as it is fundamental to a student's success in Adult Education. Poor attendance seriously affects the ability of a student to progress in a satisfactory manner.
Poor attendance is defined as being absent from class, late arrivals or early departures, on a regular basis, for any reason.
Important information regarding absences:
1. Students should communicate with their teachers or the office regarding absences.
Please call (819) 595 1226 and leave a message on our absence voice mailbox. Do not email.
In addition, medical certificates or appointment slips are required to justify absences.
2. Absences, late arrivals and early departures are recorded daily and tracked. This data will be used as evidence in a "letter of warning" or "letter of withdrawal" to be sent home, when poor attendance is being addressed.
3. In accordance with Ministry guidelines, if a student is absent for 5 days or more from all classes, they will be automatically withdrawn. A letter of withdrawal will be sent to the student's home. Furthermore, if a student has poor attendance or lateness over a period of time, they may also be withdrawn.
When a student has poor attendance or lateness over a period of time:
Step 1 – Classroom teacher addresses the issue with the student.
Step 2 – If there is no improvement, the teacher arranges a meeting with the student and the Administration. At this time the student is issued with a warning letter and will be put on probation for a specific period of time.
Step 3 – If there is no improvement, the student is withdrawn. A withdrawal letter is issued and sent home.
Students may be asked to withdraw from the school for the following reasons:
- Excessive absenteeism/tardiness.
- Excessive classroom disruption.
- Destruction or damage to school property.
- Poor, inappropriate or violent conduct.
- Possession and/or under the influence of drugs or alcohol.
- Failure to meet agreed upon targets or commitments.
*After withdrawal, a student will not be eligible for return to classes until the next available session or a period of time deemed appropriate by the Principal.
*Returning to Centre after a withdrawal:
1. Re-register and pay the $40 fee again
2. Students must submit a "request for re-admission" form to the Vice-Principal for review.


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Baltimore blasts past Oakland to start road trip
Orioles improve to 54-51 on season
Chris Davis hit a three-run shot in the first inning for his 27th homer to back rookie Tyler Wilson's impressive spot start, and the Baltimore Orioles beat the Oakland Athletics 9-2 on Monday night.
Caleb Joseph added a two-run homer in the fourth off Jesse Chavez (5-11) as Baltimore won for the eighth time in 10 games.
Orioles skipper Buck Showalter earned his 1,313th career managerial victory, tying him with Hall of Famer Ned Hanlon for 31st all-time.
Adam Jones had an RBI double in the third inning on one of his three hits, while Jimmy Paredes and Joseph hit run-scoring doubles in the fifth.
A's left fielder Coco Crisp returned from the 60-day disabled list from a neck injury that sidelined him since May 20, one of six roster moves by Oakland before the game. He batted second and went 2 for 4.


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* I love writing in colored ink. Pink and purple are my favorites.
* I think I'd love writing in glitter ink more, but I've been unable to find those types of pens.
* I'm crazy in love with the guy I married.
* I discovered, when I was a child, that bees swarm when you piss them off. My parents discovered that I was not allergic to bee stings.
* When asked what I do for a living, I answer that I'm a writer and a mom.
* I majored in political science in college with concentrations in philosophy and history.
* Once I graduated from college, I bought the toys that go in kid's meals. I totally coveted the kid's meals toys at my cubicle and never let anyone else keep one. At heart, I guess I'm still five.
* When I was in high school, I was ranked in the top twenty in the Kentucky Tennis Association.
* I never picked up a racket until I was fourteen and I only took two private lessons in my life. I had to work for two months at the K-Mart shoe department in order to pay for those lessons.
* I wore K-Mart shoes in high school because they were cheap, cute and mainly because I got a discount.
* I was attacked by a dog in second grade and repressed the memory. To this day, I only see snapshot images of the event and none of the images include blood.
* I prefer cats over dogs (unless it's my dog Rachel and then I prefer her).
* I don't remember meeting my best friend, Tiffany. She always had been a fixture in my life.
* Tiffany and I shared a locker even when we didn't have to. We also shared a dorm room in college and a basement apartment.
* She had a dog that ate out of the garbage and dragged its butt on the floor when it walked. We used the dog to test the guys we wanted to date. The guys who petted the dog were keepers.
* My husband petted the dog.
* My husband is allergic to dogs.
* The day my best friend died is one of the most devastating days of my life. I still cry over the loss.
* I don't like bugs.
* Cemeteries creep me out.
* I live one block away from a cemetery.
* As a teenager, I read the OUTSIDERS so much that the cover fell off and almost every page in the book was dog-eared.
* I hope one day someone will read my book so much that the cover falls off.
****My Faith****
Anyone who has read my books know that I write stories that are raw, gritty and contain very tough circumstances. As I've told people more than once, I write characters who face difficult problems and my characters discover that no matter how dark the situation there is always hope. I believe this to the depth of my soul.
Hope and love—those are the two most powerful things on earth.
Why do I write what I write? Because there was a time in my life I faced very dark and difficult circumstances and there were brief moments in those dark times that I didn't think hope was possible. I was wrong.
Hope and love are very real, and I found that hope and love through my faith—I am a Christian.
If you've read my books, you are already guessing correctly that I'm not your stereo-typical Christian. :-) Yes, I'm quite aware there is cursing in my books, some steamy scenes, and that my books can be very, very gritty.
I often write as raw and real as I can because that's life. But here's the thing: hope is real. So is love.
As someone once said to me, you need the darkness to enjoy the light. Is that necessarily true? I don't know, but I do know that I didn't really understand hope, love and my faith until I walked in cold darkness. Do you need to walk in cold darkness to understand faith, hope and love? No. But my path was a raw and gritty one. I was on this path due to my choices and I was on this road due to life sometime choosing my path for me.
I know that there are others out there who also walk a raw and gritty path. If you're one of those people, I'm here to tell you I'm the ghost of Christmas future—there is a way off that tough path. I'm here to tell you, things will get better and that hope and love does exist.
I'm an extremely flawed person, I have made terrible mistakes in my life, and because I am human, I will continue to make mistakes. With that said, every day I wake up and I know that love is real, that hope is real, that forgiveness is real and that my faith is real.
I believe in love. I do not believe in hate. There is no room for hate in Christianity and it is my prayer that someday all my brothers and sisters in my faith will come to agree that our faith revolves around love and understanding.
This wraps up what I believe:
Matthew 22:36-39 (NIV)
36 "Teacher, which is the greatest commandment in the Law?"
37 Jesus replied: "'Love the Lord your God with all your heart and with all your soul and with all your mind.'[a] 38 This is the first and greatest commandment. 39 And the second is like it: 'Love your neighbor as yourself.'
Favorite Quotes of Mine:
* Luke 1:37 - "For no word from God will ever fail."
* Isaiah 61:1 - "The Spirit of the Sovereign Lord is on me, because the Lord has anointed me to proclaim good news to the poor. He has sent me to bind up the brokenhearted, to proclaim freedom for the captives and release from darkness for the prisoners,"
* 1 Corinthians 13:11-13 - "11 When I was a child, I talked like a child, I thought like a child, I reasoned like a child. When I became a man, I put the ways of childhood behind me. 12 For now we see only a reflection as in a mirror; then we shall see face to face. Now I know in part; then I shall know fully, even as I am fully know.13 And now these three remain: faith, hope and love. But the greatest of these is love."
* John 15:12-13 - "12 My command is this: Love each other as I have loved you. 13 Greater love has no one than this: to lay down one's life for one's friends. "
* John 8:32 - "Then you will know the truth, and the truth will set you free."
* Isaiah 43:18 - "Forget the former things; do not dwell on the past."
* Galatians 5:13-14 13 You, my brothers and sisters, were called to be free. But do not use your freedom to indulge the flesh[a]; rather, serve one another humbly in love. 14 For the entire law is fulfilled in keeping this one command: "Love your neighbor as yourself."
* Psalm 62:6 - "Truly he is my rock and my salvation; he is my fortress, I will not be shaken."
*Ecclesiastes 4:12 - "Though one may be overpowered, two can defend themselves. A cord of three strands is not quickly broken."
*Psalm 112:7-8 - "They will have no fear of bad news; their hearts are steadfast, trusting in the Lord. Their hearts are secure, they will have no fear; in the end they will look in triumph over their foes.


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Introduction: Strip Painting Installation Instructions
These instructions are intended for those who have been given the task of installing my artwork, Strip Painting, 2015.
I sincerely thank you for your help.
As someone who has also installed other artists' work in galleries, museums, and art fairs, I understand the importance of clear, concise instructions for getting the job done in a timely fashion. I chose to write an instructable because it is an ideal format for this purpose and can be accessed with a smart phone on site.
About Strip Painting:
Strip Painting plays off of the notion of "drip painting" made famous by artists like Jackson Pollock and Janet Sobel. These artists achieved a new type of pictorial space, one that was no longer tied to representation but drew attention to the act of painting itself. Writing about the "all over" composition in this type of work, Clement Greenberg stated in "The Crisis of the Easel Picture" (1948):
"...the "all-over" painter renders every element and every area of the picture equivalent in accent and emphasis. Like the twelve-tone composer, the "all-over" painter weaves his work of art into a tight mesh whose scheme of unity is recapitulated at every meshing point."
The Strip Painting takes this line of thinking literally, by reducing the painting to narrow strips and weaving them "into a tight mesh" onto custom wooden wall brackets. A strip painting is able to cover maximum wall space using minimal amount of painting. It is lightweight and the strips are easily detached and rolled up individually for storage and transport.
Tools You Will Need:
Cordless Screwdriver
Phillips and Hex bit
Measuring Tape
Blue Painters tape
Artwork Includes:
Four custom wooden brackets, identical
Screw for attaching brackets to wall, phillips head, 24 needed, 30 supplied
16 painting strips, rolled individually, numbered 1 through 16
16 painting strips, rolled individually, unnumbered
Machine screws for attaching strips to brackets, hex head, .5 inch long, thread size 1/4"-20, 32 needed, 40 supplied
Step 1: Know the Final Dimensions, Find the Centerpoint on the Wall.
The piece is 100" tall by 66.25" wide when installed. I recommend installing it at least 18" from the ground, but this might change depending on the height of the ceiling and the tastes of the curator.
Locate the center point where the finished piece will be and mark on wall with blue tape. Mark a cross on the tape that designates the center point.
Step 2: Install Wooden Brackets on Wall
From the center point, use the level and measuring tape to measure 50" directly above and below, marking the wall with blue tape and pencil. From these points, use the level to measure 8.125" to the left and 8.125" to the right, and mark with blue tape and pencil.
Install the wooden brackets on the wall using the level and your blue tape marks as a guide according to the diagram. The four brackets are identical. Each bracket has 6 holes for the screws to go through that attach it to the wall. Each bracket has 16 holes for attaching the painting strips to the bracket. One side of the holes has a brass insert, the other doesn't. Install the brackets so the brass inserts are facing up on the upper brackets, and facing down on the lower brackets.
Step 3: Attach Strips to Upper Left Bracket and Lower Right Bracket in Numerical Order
Next you'll be attaching the first set of strips on the upper left and lower right brackets. The strips are in rolls numbered 1 through 16, to be attached according to the diagram.
Begin with roll number 1 which gets attached to the upper left bracket. You can leave the strip rolled up until all the rolls are installed on the upper bracket. This helps them from getting tangled. Attach the strip to the brass insert using one of the machine screws provided. Just place the screws in by hand for now, later you can tighten them with the screwdriver and hex bit.
Once all sixteen strips have been fastened to the upper left bracket, begin on either end and unroll and attach the first strip to the lower right bracket in the same fashion as above. Unroll and attach each strip one by one until all 16 are attached to both brackets.
Step 4: Attach Unnumbered Strips to Upper Right Bracket and Weave Through Other Strips
The second set of strips are unnumbered. The look of this half of the piece is determined by your choices. Install the second half the same way as the first, making sure to weave the strips into the first set before attaching them to the lower left bracket. The strips are woven together using a simple plain weave, over and under, over and under.
Step 5: Tighten the Machine Screws and You Are Done!
Once all the strips are attached and woven together, you can tighten the machine screws with the screwdriver with hex bit. No need to over tighten them, though. The strips should be taught, not droopy.
Thanks again for your help, I hope to have a drink with you at the opening reception!
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8 Discussions
i love your work! is there somewhere i can visit online to see more examples of this unique type of medium, or perhaps purchase some of your personal work?
Hi! Thanks for the compliment. You can view my work at www.miguelarzabe.net
This looks beautiful! :)
I appreciate your comment!
Good idea for sharing instructions
Thank you, I hope it is useful for others.
Beautiful, well done!
Muchas gracias.


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My Brother was born 5.5 years later and I stayed at Nan Nan's in the local village Snainton. A young man called Charles walked me to school as he fancied me.
When my Mother's bridesmaid had her Daughter she decided to call her Linda as well.
From 1966 to1971 I attended Scalby County Secondary School and was a Monitor. During the time I spent at this School the two Linda's met a big headed Barsteward which is a polite way who fancied himself called Colin who use to chase me along the path and caught up with me behind the prefabs before the teachers got there. I wonder why he caught me (lol) The teachers went across the field.
The headboy called Shaun when we went to stay at the Youth Hostel Association carried my Suit cases up the many steps to the top of the hill hey he fancied me to (lol).
When I first left School I worked at F.W.Woolworth Ltd.
I became friendly with one of the under Managers which they did not like as their laws stated you were not allowed to go out with them. When we visited the local Y.M.C.A. his flat was across the road and it was a long way to walk to the bus station so he like a gentleman should and made sure that I got to the bus safely. We got reported that we were seeing each other. Perhaps there was a lot of jealousy.
I was engaged to a young man called Trevor and I broke his heart as I wanted a Career first.
I decided to go into catering as my parents had a small guest house and I had to serve on the tables and help with the Saturday change over and earn my pocket money. I went and to learnt silver service at The Station Hotel at York. I was supposed to be on the training station for 1 Month and after one week I was given a station of my own which is the one the Managers use for their business. What a frightening thought.
My name is Linda. In early 1998 I had to leave work through illness. I got the chance to go on College courses as a mature student doing computer software. I did not know how to switch a computer on and finding my way around a keyboard was head banging stuff. I had to learn how to study again. I overcame these hurdles and during the next 4 years I got an Intermediate Diploma and then went on got letters after my name. I was very pleased with myself.
I compiled a cook book on recipes around the world and raised funds for Children in Need and raised over £ 90.
My Father died Ist May 2002 and 8 months later my Mother died 22 nd January 2003. 8 months later 16 th September 2003 I fell ill.
All decorated cards are decorated by myself and all computer cards are designed by myself. I have all so made the 3D Embellishments.
In 2004 I joined a local charity and went on their helpers training. Police checks and everything I am now starting on learning Book Keeping and learning Net Working.
Interests :
Country Music
Mental Health U.K.
Needy Children
Charity Work
Health and Wellness
The letters I have are A.C.I.T.P. My version is Certified Black Hole of Calcutta. Which is fun and a lot easier to remember.
(Associate Certified Informational Technology Professional)
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