text:
This paper details how Elvax 40W can be used as a slow-release method for drug delivery to the adult rat retina. The protocol for preparing, loading, and delivering the drug-resin complex to the eye is described.
Diseases of the retina are difficult to treat as the retina lies deep within the eye. Invasive methods of drug delivery are often needed to treat these diseases. Chronic retinal diseases such as retinal oedema or neovascularization usually require multiple intraocular injections to effectively treat the condition. However, the risks associated with these injections increase with repeated delivery of the drug. Therefore, alternative delivery methods need to be established in order to minimize the risks of reinjection. Several other investigations have developed methods to deliver drugs over extended time, through materials capable of releasing chemicals slowly into the eye. In this investigation, we outline the use of Elvax 40W, a copolymer resin, to act as a vehicle for drug delivery to the adult rat retina. The resin is made and loaded with the drug. The drug-resin complex is then implanted into the vitreous cavity, where it will slowly release the drug over time. This method was tested using 2-amino-4-phosphonobutyrate (APB), a glutamate analogue that blocks the light response of the retina. It was demonstrated that the APB was slowly released from the resin, and was able to block the retinal response by 7 days after implantation. This indicates that slow-release drug delivery using this copolymer resin is effective for treating the retina, and could be used therapeutically with further testing.
The treatment of chronic diseases such as diabetes and high blood pressure presents many challenges, as these diseases generally require treatment for extended periods of time, often for life. This has called for the development of slow release drug delivery systems, which reduce the need for frequent dosing. The effectiveness of these slow release methods has been demonstrated through the development of insulin pumps, to reduce the number of insulin injections needed to treat diabetes. Chronic diseases of the eye, particularly those affecting its inner layers, require the frequent administering of drugs through invasive procedures. One such chronic disease affecting the human eye is age related macular degeneration (AMD). It affects the central retina, which is a layer of neural tissue situated at the back of the eye responsible for initiating vision. AMD is the leading cause of blindness in the Western World1. A particular challenge with treating retinal diseases is that the drug is required to reach this deep layer in the eye, which often requires invasive methods of delivery. Drugs are usually administered to the vitreous chamber and retina using intravitreal injections. However, with each injection there is a risk of postinjection complications, including endophthalmitis, retinal detachment, cataract and vitreous hemorrhaging2. This risk is multiplied with every reinjection of the drug.
Reducing the need for multiple injections would be a major benefit in the treatment of AMD. To treat the wet form of AMD, where new vessel growth is a hallmark, the established therapeutic strategy is to target the endothelial growth factors (VEGF) using VEGF inhibitors3. At present, these are delivered through repeated intravitreal injections. Similarly, in the treatment of macular oedema, a common complication of diabetic retinopathy, corticosteroids are delivered through repeated injections4. Delivery of these drugs through slow release methods could indeed reduce the risk of postinjection complications.
The notion of a slow release drug delivery system was first described using a silicone rubber vehicle to deliver small molecules into animal tissues5. Since then, other slow release methods have been developed to deliver larger molecules, several of which have been tested in the eye. Particle carriers, such as biodegradable microspheres, poly-lactide-co-glycolide (PLGA) nanoparticles and phospholipid vesicles (liposomes) can be useful as delivery vehicles6,7. PLGA nanoparticles and liposomes have been compared in an in vitro environment for their ability to deliver anti-cancer agents across the sclera over time7. Both vehicles were effective in slowly releasing the drugs. However, the study was only conducted in an in vitro environment. Bochot et al. (2002)8 tested the efficacy of liposomes to deliver molecules to the retina in vivo. They have demonstrated that liposomes successfully deliver small oligonucleotides to the rabbit retina. The authors suggested that liposomes could be beneficial in the treatment of retinal diseases8. However, the nature of these vesicles to float in the vitreous means that they will likely blur or impair vision9.
Okabe et al. (2003)10 used non biodegradable polymer discs made up of 33% ethylene-vinyl acetate to apply beta-methasone in rabbits. They implanted discs into a scleral pocket and demonstrated the effective release of the drug into the vitreous and retina for up to one month10. However, in this particular protocol, the implant was relatively large and rigid, and required a more complex surgical procedure including a large scleral incision and suturing.
An earlier study investigated the tissue response to various polymer vehicles by implanting them into the cornea of rabbit eyes, and found that ethylene-vinyl acetate copolymers washed in alcohol did not cause inflammation or irritation. These complexes were shown to sustain the delivery of larger compounds into animal tissues for extended periods of time, some exceeding up to 100 days dependent on the drug11. One such type of copolymer resin has been developed industrially in the form of Elvax 40W (40% by weight ethylene-vinyl acetate comonomer content with a 'W' amide additive to improve pellet handling). This copolymer resin is an inert substance which is stable at both room and body temperature. It has not been shown to cause allergies or toxicity in biological tissue. This resin has effectively delivered a large variety of drugs in different experimental models that investigate the functions of various systems, such as the mammary ductal system12, the primary auditory cortex13,14, and the frog visual system15. This resin has also been used in the eye to deliver drugs to developing turtle16,17, chicken embryo18,19, and adult ferret retinas20. In the rat central nervous system, the resin has only been used in the brain21-23, but its use in the rat eye has not been documented.
The advantages of using this copolymer resin to deliver drugs slowly to the retina over other methods, is that it is a stable compound that does not cause inflammation or irritation in the eye. Unlike particle carriers, the drug-resin complex would not impair vision after implantation, as it usually remains at the site of delivery instead of floating in the vitreous. It would only require a simple implantation process into the vitreous cavity close to the limbus of the eye, and would not require suturing after implantation. Recently, there has been an emergence in several novel delivery systems, such as encapsulated cell technology (ECT)24,25, hydrogels26, and microfilms27. However, the method used in the current study for preparing and delivering the drug-resin complex is both easy to follow and inexpensive, thereby being more advantageous for use in a basic research environment. The challenge of using this complex to deliver long-term drug treatments is to determine the optimal concentration of the drug that will maximize the therapeutic benefits of having fewer intravitreal injections.
This paper aims to demonstrate the use of the drug-resin complex for long-term treatment of the adult rat retina. The efficacy of this mode of delivery is tested using the glutamate analogue 2-amino-4-phosphonobutyrate (APB) as the drug. APB blocks the light response of the ON bipolar cells by mimicking glutamate, an endogenous neurotransmitter in the retina28. When APB competes with glutamate for its receptor, it blocks the light response. APB has been used in physiological studies to control retinal function and measure its effect using electrophysiological methods such as electroretinography (ERG). In previous studies, APB has been used for both short-term29 and long-term treatment of the developing retina; the latter involved giving a single dose via intraocular injection daily for 30 days30. The same amount of APB (0.092 mg in sterile saline, at a concentration of 50 µM) was used for all injections as suggested in previous work28,29,31. We chose APB to test the drug-resin complex as a slow-release vehicle to deliver drugs into the eye. The methods outlined in this study are similar to previously described methods involving the preparation of the drug-resin complex16,32; however we also detail its use specifically in the adult rat eye. After surgical implantation of the APB-loaded resin into the eye, ERG was performed to establish whether APB abolishes the retinal light response, and therefore whether APB has been successfully released into the vitreous and retina.
Subscription Required. Please recommend JoVE to your librarian.
All experiments conducted were in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and with the approval of the University of L'Aquila Animal Ethics Committee and the Australian National University Animal Experimentation Ethics Committee. Adult rats (P100-200) were used throughout this study.
1. Prepare the Copolymer Resin Pellets
- Place 20 Elvax 40W pellets into a small glass beaker in the fume cupboard.
- Fill the beaker with 100% ethanol ensuring to cover all pellets.
- Cover the beaker with Parafilm, and soak the pellets in 100% ethanol at room temperature for 7-10 days. NOTE: From this step onwards, do not use metal or plastic to handle or store the resin.
2. Prepare the Drug Solution for Loading
- Dissolve the drug of choice in 0.1% dimethyl sulfoxide (DMSO). Make the drug solution up to 40 µl in volume. Load the resin with three times the concentration of the drug that would be given in a single dose.
3. Prepare and Load the Drug-resin Complex
- Transfer the 20 washed pellets to another small glass beaker. Dissolve the pellets in 4 ml dichloromethane for approximately 45 min. Cover the beaker with Parafilm while the pellets are dissolving.
- Prepare a solution of Fast Green FCF by dissolving it in 0.1% DMSO to a concentration of 0.001 mg/ml.
- Take one pipette, and draw up 40 µl of the drug solution prepared earlier. Load another pipette with 40 µl of Fast Green solution.
- Add the two solutions simultaneously into the beaker. Mix rapidly using a glass stirring rod until the green dye is uniformly distributed throughout the mixture.
- Promptly transfer the beaker onto dry ice for 10 min to fast freeze the resin.
- Set up an "evaporative chamber" to evaporate the solvent. Fill a larger container up to one-third with calcium sulfate pebbles. Create a well in the pebbles, and carefully place the beaker in the well so that the pebbles reach up to half of the beaker, to stabilize the beaker in the chamber.
- Cover the larger outside container with Parafilm.
- Transfer the evaporative chamber to the freezer. Keep at -20 °C for 2-3 weeks.
4. Prepare the Drug-resin Complex for Surgical Implantation
- Remove the evaporative chamber from the freezer. Transfer the solid drug-resin block to a glass dish kept on ice.
- With the aid of a microscope or loupe, cut a piece approximately 0.05 mm in diameter and 0.1 mm in length from the block using a trephine or punch tool.
- Wrap the remainder of the block in freeze-proof material (e.g., aluminum foil) and keep at -20 °C until required.
5. Surgically Implant the Drug-resin Complex into the Rat Eye
- Prepare the animal for surgery. Anesthetize using an intraperitoneal injection of a mixture of ketamine (100 mg/kg body weight) and xylazine (12 mg/kg body weight). Observe the animal until full anesthesia is achieved. NOTE: The animal should have a loss of movements (except breathing), and a loss of corneal and toe pinch reflexes.
- Transfer the fully anesthetized animal to the surgery table. Use mydriaticum (e.g., atropine sulphate), and local anesthetic (e.g., tetracaine hydrochloride) drops on the eye(s) undergoing surgery. Apply artificial tears to keep the cornea moist during surgery. Use disposable needles and sterile instruments throughout the surgery.
- Stabilize the eye using a pair of blunt curved forceps after the pupil is fully dilated. Use an operating microscope to make a full depth puncture wound to reach the vitreous, approximately 2 mm from the limbus using a 25 G needle.
- Insert the prepared piece of the drug-resin complex into the puncture wound using a pair of fine, pointy forceps (e.g., tweezers) that are able to penetrate the puncture site.
- As prophylaxis for bacterial infection, apply antibiotic ointment to the eye, to prevent inflammation as well as preventing corneal dryness while the animal is recovering.
- Observe the animal until full recovery. Do not leave the animal unattended until it has regained consciousness. Do not return the animal to the company of other animals until fully recovered. If the animal shows signs of stress or discomfort, analgesics should be used according to the approved ethics protocol.
- Follow the usual experimental procedures for collection and analysis of the tissue.
- At the completion of experiments, euthanize the animal with an intraperitoneal injection of a pentobarbital (60 mg/kg body weight) 2% lidocaine hydrochloride solution.
NOTE: Lignocaine Hydrochloride minimizes local discomfort.
Subscription Required. Please recommend JoVE to your librarian.
Representative Results
Full-field electroretinography (ERG) was used to detect the effect of APB on the retina. For details on how the ERG was performed, refer to the following studies33,34. In brief, after anesthetizing the animal, a gold electrode was placed on the cornea of the eye, and the reference electrode was placed in the anterior scalp between the eyes, to record the electrical activity of the retina. Stimuli were generated using an electronic flash unit and the intensity ranged from 10 to 106 photoisomerization, which represents the number of photosensitive molecules activated per rod. In these experiments, ERG recordings were taken at 1 and 7 days post procedure. The right eye of each animal was implanted with the drug-resin complex, whereas the fellow left eye was used as a non treated internal control.
Figure 1 shows the changes in the amplitude of the b-wave with an increase in photoisomerization per rod (φ). In the control eye, the amplitude of the b-wave increases as the flash intensity increases. In Figure 1A, retinal response recordings are given 1 day after implantation. The implanted eye showed a similar amplitude pattern to the control eye. The amplitude difference between the two eyes became significantly different at 106 φ (p <0.0001). This indicates that at this early stage, the drug-resin complex does not have an effect on retinal response until reaching higher flash intensities. Figure 1B demonstrates changes in the ERG response at 7 days after implantation. The b-wave is abolished at all levels of flash intensity in the implanted eye, when compared to the control eye, which has amplitudes comparable to the 1 day recordings. This is significantly different to all amplitudes for the implanted eye at 1 day (p <0.0001). This shows that the retinal response has been abolished by (one week) after the implantation of the drug-resin complex into the eye.
Figure 1. The amplitude of the b-wave at 1 day and 1 week after the implantation of the drug-resin complex. For both panels, the control eye is shown in orange (squares), whereas the implanted eye is shown in green (circles). The symbol * represents significance at this point between the two eyes, where p <0.0001. For all groups, n = 5. (A) The amplitude of the b-wave 1 day after the implantation of the drug-resin complex into the eye. The amplitude of the b-wave in relation to the flash intensity (photoisomerization per rod φ) is an indicator of retinal response. There is no significant difference in the b-wave amplitude between the eyes at all flash intensities except at 106φ, where the implanted eye has a lower amplitude. (B) The amplitude of the b-wave 1 week (7 days) after implantation. The amplitudes recorded from both eyes are significantly different at all flash intensities. In the implanted retina, amplitude of b-wave was not measurable at any stimulus intensities.
Subscription Required. Please recommend JoVE to your librarian.
This paper demonstrated the use of a drug-resin complex for the slow-release delivery of drugs to the retina. We aimed to present a method which is relatively inexpensive and easy to apply in a small animal model.
Given that the function of APB is to act as a glutamate analogue, it will block the retinal response in the eye. The results show that APB caused the blockage of the retinal response by one week postimplantation. This indicates that APB was successfully released into the vitreous and retina, and that its effect was limited to the eye that the drug-resin complex was implanted into. The full effect of the drug became obvious one week after implantation (Figure 1B). Figure 1A shows that at 1 day after implantation, a significant reduction of the b-wave was only present at the 106φ intensity in the implanted eye. This may indicate that at this early time point, only the cone pathway, which responds to higher light intensities, was affected. To determine the length of time that the resin can deliver the APB to the retina, further time points should be investigated. It is proposed that recordings at further time points within the 0-7 day span should be taken, for example, at 3 and 5 days after implantation, to determine exactly when the retinal response is fully abolished. Additionally, recordings should be taken beyond 7 days, to determine how long the APB is released for. This would be evident as the retinal response would continue to be abolished if APB is continually being released slowly into the vitreous.
In this study, the drug-resin complex was found to be safe for delivery to the retina. APB was dissolved in 0.1% DMSO. At concentrations equal to or higher than 0.6%, DMSO can cause retinal toxicity. However, 0.1% DMSO has been found to be a safe vehicle for the delivery of compounds to the rat eye35, and this concentration was used throughout all experiments in this study. The implants were inserted transclerally into the vitreous cavity at the ora serrata. The implants were observed to remain at the ora serrata in cryosections of the rat eye, which was collected after the ERG recordings were made. This positioning of the implant guarantees that the drug-resin complex does not cause vision impairment. This method involving the drug-resin complex has been tested with other compounds (publication in preparation), and it was found that no structural or functional damage to the eye was observed up to two months after implantation. A total of 60 animals were implanted, and in 90% of cases the implant position remained unchanged.
Several modifications can be made to the technique if necessary. In these experiments, three times the concentration of a single dose of APB was loaded into the resin (50 µM x 3 = 150 µM), and this should be used as a guideline for preparing the drug solution for loading. However, the effective concentration of the drug in the resin is variable, and should be tested and modified accordingly. The time points where analysis is conducted can also be modified, to determine how long the resin is effective at releasing the drug. In these experiments, it was shown that the stable effective dose of the drug is delivered through the drug-resin complex for at least one week, so it is recommended that analysis of the tissue is performed at least one week after the implantation. During the drug loading step, Fast Green was used to ensure that the mixture had homogenized. Other biologically safe color dyes can be used as an alternative to Fast Green, to visualize the uniformity of the drug within the resin. A limitation of this method is that the Fast Green dye is the only measure of uniform mixing. This technique could also be tested using alternative animal models and drugs, with only minor adjustments to the method. If a higher amount of the drug needs to be delivered, a larger size implant may be necessary. If successful in these models, this copolymer resin could be considered as a therapeutic option for delivering drugs slowly to the retina to have a continued effect over time.
There are several critical steps within the protocol where extra care should be taken. Plastic and metal instruments should not be used throughout the synthesis and handling of the resin. This prevents contamination of the polymer, as dichloromethane can dissolve plastic and corrode metal. Proper dryness of the drug-resin block should be achieved before using it for surgical implantation. Ensure that the beaker containing the drug and resin is fully exposed to the calcium sulfate in order to evaporate the solvent. Alternatively, a lyophilizer can be used to freeze dry the drug-resin. The drug-resin block is ready for use when the solvent has completely evaporated, and only a solid bluish block remains in the beaker. When cutting a piece from the block for implantation, each piece should only be prepared immediately before the surgery as the pieces are very small and are difficult to store. A trephine or punch tool should be used to cut pieces of equal size, ensuring that each piece contains approximately the same amount of the drug. Finally, when inserting the piece into the puncture wound made by the 25 G needle, use fine, pointy tweezers to carefully guide the piece into the wound. An operating microscope or high power loupe should be used as a visual aid.
Only hydrophilic drugs and compounds have been tested using this method. The drug-resin complexes have not shown any undesirable precipitation or byproduct formation, when the drug was mixed with the polymer and the dichloromethane. It is suggested that with each new drug, the concentration, half-life of effect, and any possible reactions with the resin mixture should be tested by the user to determine if the method is suitable.
Subscription Required. Please recommend JoVE to your librarian.
The authors have nothing to disclosure.
This research was supported by the Australian Research Council through the ARC Centre of Excellence in Vision Science (CE0561903), the National Health and Medical Research Council (1049990), and Progetto regionale speciale multiasse 'Reti per l'Alta Formazione' - PO FSE Abruzzo 2007-2013 - Azione 4.II.iii.
|Catalog Number
|Elvax 40W Pellets
|Du Pont, DE, USA
|Dimethyl sulfoxide (DMSO)
|Sigma-Aldrich Co. LLC., MO, USA
|2-amino-4-phosphonobutyric acid (APB)
|Sigma-Aldrich Co. LLC., MO, USA
|Sigma-Aldrich Co. LLC., MO, USA
|Fast Green FCF
|Sigma-Aldrich Co. LLC., MO, USA
|Drierite, calcium sulfate
|Sigma-Aldrich Co. LLC., MO, USA
|Ketamine, Ilium Ketamil
|Troy Laboratories Pty. Ltd., NSW, Australia
|Xylazine, Ilium Xylazil-20
|Troy Laboratories Pty. Ltd., NSW, Australia
|Atropine sulphate, Minims eye drops
|Bausch & Lomb Pty. Ltd., NSW, Australia
|Tetracaine hydrochloride, Minims eye drops
|Bausch & Lomb Pty. Ltd., NSW, Australia
|Chloramphenicol, Chlorsig ointment
|Aspen Pharma Pty. Ltd., NSW, Australia
|Pentobarbital, Lethabarb
|Virbac Australia Pty. Ltd., NSW, Australia
|Lidocaine hydrochloride, Ilium Lignocaine-20
|Troy Laboratories Pty. Ltd., NSW, Australia
|Uni-Core Punch Tool
|World Precision Instruments Inc., FL, USA
|Curved Forceps
|World Precision Instruments Inc., FL, USA
|Operating Microscope, Zeiss OPMI 99
|Zeiss, West Germany
|25 G Insulin needle
|Terumo Corp., Tokyo, Japan
|Dumont tweezers
|World Precision Instruments Inc., FL, USA
- Access Economics, The global economic cost of visual impairment. AMD Alliance International. 26-27 (2010).
- Jager, R., Aiello, L., Patel, S., Cunningham, E. Risks of intravitreous injection: A comprehensive review. Retina. 24, (5), 676-698 (2004).
- Rosenfeld, P. J., et al. Ranibizumab for neovascular age-related macular degeneration. New England Journal of Medicine. 355, (14), 1419-1431 (2006).
- Cunningham, M. A., Edelman, J. L., Kaushal, S. Intravitreal steroids for macular edema: The past, the present, and the future. Survey of Ophthalmology. 53, (2), 139-149 (2008).
- Folkman, J., Long, D. M. The use of silicone rubber as a carrier for prolonged drug therapy. Journal of Surgical Research. 4, (3), 139-142 (1964).
- Herrero-Vanrell, R., Refojo, M. F. Biodegradable microspheres for vitreoretinal drug delivery. Advanced Drug Delivery Reviews. 52, (1), 5-16 (2001).
- Kim, E., et al. Human scleral diffusion of anticancer drugs from solution and nanoparticle formulation. Pharmaceutical Research. 26, (5), 1155-1161 (2009).
- Bochot, A., et al. Intravitreal delivery of oligonucleotides by sterically stabilized liposomes. Investigative Ophthalmology & Visual Science. 43, (1), 253-259 (2002).
- Bochot, A., Fattal, E. Liposomes for intravitreal drug delivery: A state of the art. Journal of Controlled Release. 161, (2), 628-634 (2012).
- Okabe, K., Kimura, H., Okabe, J., Kato, A., Kunou, N., Ogura, Y. Intraocular tissue distribution of betamethasone after intrascleral administration using a non-biodegradable sustained drug delivery device. Investigative Ophthalmology & Visual Science. 44, (6), 2702-2707 (2003).
- Langer, R., Folkman, J. Polymers for the sustained release of proteins and other macromolecules. Nature. 263, (5580), 797-800 (1976).
- Silberstein, G. B., Daniel, C. W. Elvax 40P implants: Sustained, local release of bioactive molecules influencing mammary ductal development. Developmental Biology. 93, (1), 272-278 (1982).
- Smith, A. L., et al. An investigation of the role of auditory cortex in sound localization using muscimol-releasing Elvax. European Journal of Neuroscience. 19, (11), 3059-3072 (2004).
- Anomal, R., De Villers-Sidani, E., Merzenich, M. M., Panizzutti, R. Manipulation of BDNF signaling modifies the experience-dependent plasticity induced by pure tone exposure during the critical period in the primary auditory cortex. PloS one. 8, (5), e64208 (2013).
- Tu, S., Butt, C. M., Pauly, J. R., Debski, E. A. Activity-dependent regulation of substance P expression and topographic map maintenance by a cholinergic pathway. The Journal of Neuroscience. 20, (14), 5346-5357 (2000).
- Sernagor, E., Grzywacz, N. M. Influence of spontaneous activity and visual experience on developing retinal receptive fields. Current Biology. 6, (11), 1503-1508 (1996).
- Leitch, E., Coaker, J., Young, C., Mehta, V., Sernagor, E. GABA type-A activity controls its own developmental polarity switch in the maturing retina. The Journal of Neuroscience. 25, (19), 4801-4805 (2005).
- Park, C. M., Hollenberg, M. J. Basic fibroblast growth factor induces retinal regeneration in vivo. Developmental Biology. 134, (1), 201-205 (1989).
- Whitsel, A. I., Johnson, C. B., Forehand, C. J. An in ovo chicken model to study the systemic and localized teratogenic effects of valproic acid. Teratology. 66, (4), 153-163 (2002).
- Prusky, G. T., Ramoa, A. S. Novel method of chronically blocking retinal activity. Journal of Neuroscience Methods. 87, (1), 105-110 (1999).
- Colonnese, M. T., Constantine-Paton, M. Chronic NMDA receptor blockade from birth increases the sprouting capacity of ipsilateral retinocollicular axons without disrupting their early segregation. The Journal of Neuroscience. 21, (5), 1557-1568 (2001).
- Oliveira-Silva, P., et al. Matrix metalloproteinase-9 is involved in the development and plasticity of retinotectal projections in rats. Neuroimmunomodulation. 14, (3-4), 144-149 (2007).
- Trindade, P., et al. Evidence for a role of calcineurin in the development of retinocollicular fine topography. Neuroscience Letters. 487, (1), 47-52 (2011).
- Kauper, K., et al. Two-year intraocular delivery of ciliary neurotrophic factor by encapsulated cell technology implants in patients with chronic retinal degenerative diseases. Investigative Ophthalmology & Visual Science. 53, (12), 7484-7491 (2012).
- Birch, D. G., Weleber, R. G., Duncan, J. L., Jaffe, G. J., Tao, W. Randomized of ciliary neurotrophic factor delivered by encapsulated cell intraocular implants for retinitis pigmentosa. American Journal of Ophthalmology. 156, (2), 283-292 (2013).
- Rauck, B. M., et al. Biocompatible reverse thermal gel sustains the release of intravitreal bevacizumab in vivo. Investigative Ophthalmology & Visual Science. 55, (1), 469-476 (2014).
- Liu, Y. C., Peng, Y., Lwin, N. C., Venkatraman, S. S., Wong, T. T., Mehta, J. S. A biodegradable, sustained-released, prednisolone acetate microfilm drug delivery system effectively prolongs corneal allograft survival in the rat keratoplasty model. PloS one. 8, (8), e70419 (2013).
- Slaughter, M. M., Miller, R. F. 2-amino-4-phosphonobutyric acid: a new pharmacological tool for retina research. Science. 211, (4478), 182-185 (1981).
- Bodnarenko, S., Jeyarasasingam, G., Chalupa, L. Development and regulation of dendritic stratification in retinal ganglion cells by glutamate-mediated afferent activity. The Journal of Neuroscience. 15, (11), 7037-7045 (1995).
- Deplano, S., Gargini, C., Maccarone, R., Chalupa, L. M., Bisti, S. Long-term treatment of the developing retina with the metabotropic glutamate agonist APB induces long-term changes in the stratification of retinal ganglion cell dendrites. Dev Neurosci. 26, (5-6), 396-405 (2004).
- Horton, J., Sherk, H. Receptive field properties in the cat's lateral geniculate nucleus in the absence of on-center retinal input. The Journal of Neuroscience. 4, (2), 374-380 (1984).
- Smith, A. L., Cordery, P. M., Thompson, I. D. Manufacture and release characteristics of Elvax polymers containing glutamate receptor antagonists. Journal of Neuroscience Methods. 60, (1), 211-217 (1995).
- Gargini, C., Bisti, S., Demontis, G. C., Valter, K., Stone, J., Cervetto, L. Electroretinogram changes associated with retinal upregulation of trophic factors: observations following optic nerve section. Neuroscience. 126, (3), 775-783 (2004).
- Maccarone, R., Di Marco, S., Bisti, S. Saffron supplement maintains morphology and function after exposure to damaging light in mammalian retina. Investigative Ophthalmology & Visual Science. 49, (3), 1254-1261 (2008).
- Tsai, T., Bui, B., Vingrys, A. Dimethyl sulphoxide dose-response on rat retinal function. Documenta Ophthalmologica. 119, (3), 199-207 (2009).


text:
WestJet today celebrated the opening of its new Dreamliner maintenance hangar at its hub at Calgary International Airport (YYC). Member of Parliament, Kent Hehr, City of Calgary Councillor, Jyoti Gondek, Calgary Airport Authority President and CEO, Bob Sartor, 1500 WestJet employees and members of WestJet's executive team were on hand for a ribbon-cutting ceremony. WestJet also welcomed members of the Tsuut'ina Nation who led a blessing of the hangar at the end of the ceremony.
WestJet today celebrated the opening of its new Dreamliner maintenance hangar at its hub at Calgary International Airport (YYC) (CNW Group/WESTJET, an Alberta Partnership)
"Today marks another significant step in WestJet's journey as a global network carrier," said Ed Sims, WestJet President and CEO. "This state-of-the-art facility provides the perfect environment for our highly skilled aviation maintenance engineering teams to continue to ensure the safety and comfort of our 25 million annual guests."
WestJet's $50 million investment in the 125,000-square-foot hangar is in addition to the airline's announcement that it will be the first Canadian airline to base its Dreamliner aircraft out of YYC Calgary International Airport. The first three 787 Dreamliner aircraft launch from Calgary on non-stop routes to London, Paris and Dublin on April 28, May 17 and June 1 respectively. Both investments are expected to generate significant economic output and support thousands of direct and indirect jobs in Alberta. WestJet also recently expanded the list of destinations it serves to 67 with the addition of non-stop Atlanta, Portland and Austin routes from Calgary. WestJet remains the airline with the most destinations, seats and departures out of YYC.
WestJet's hangar accommodates one 787-9 Dreamliner aircraft or up to four 737 aircraft. It increases the airline's campus size to 500,000 square feet or 11 acres. The hangar allows for indoor maintenance on the airline's Dreamliner aircraft and can accommodate up to 130 members of the WestJet technical operations teams with workspace and tools to maintain the reliability and safety of WestJet's fleet.
Additional Quotes
"The opening of this hangar today is another example of how our biggest airline partner, WestJet and the Calgary Airport Authority are working hand-in-hand for the good of the city," said Bob Sartor, The Calgary Airport Authority President and CEO. "WestJet's investment in its Calgary hub has a direct impact on jobs, the economy and all of the opportunities that come with vital air service connecting travellers to other economies and markets."
"Selecting Calgary as the hub for its 787 Dreamliner fleet, and building out the hangar facility, speaks to the confidence WestJet has in the Calgary and Alberta market," said Mary Moran, President & CEO, Calgary Economic Development. "WestJet continues to embody Calgary's entrepreneurial spirit and highlights the opportunity to grow incredible companies here. Our city is a gateway to regional and global markets and having an airline hub makes us a more attractive market for investment through significant passenger and cargo growth."
"CANA is honoured to deliver the new WestJet Dreamliner Hangar to our partners at WestJet," said Fabrizio Carinelli, CANA Construction Ltd. President. "The state-of-the-art hangar was built for safety and efficiency for both WestJet guests and employees but also importantly, its construction generated more than 300,000 people hours to the local economy in Calgary. CANA is privileged to work with WestJet as they go above and beyond to invest in our community and fly to new global destinations."
"Stantec is proud to join our partners at WestJet and the Calgary Airport Authority to celebrate the opening of the Dreamliner hangar," said Russ Wlad, Executive Vice-President, Canada, Stantec. "This project, which was designed by our local team of architects and engineers, will support and promote growth at one of Canada's busiest airports. The Dreamliner will take passengers to new destinations around the world and will create opportunities to bring people to Calgary and Alberta."

subset: commoncrawl


text:
跳到导航 跳到搜索
Mairead Maguire[1][2]
|Mairead Corrigan
|Mairead Corrigan Maguire
|Jackie Maguire
|2 (5)[1][4]
|Anne Maguire(姐妹)
Pacem in Terris (1990)
梅里德·科里根·麥奎爾(Mairead Corrigan Maguire ,1944年1月27日-)是一名北爱尔兰社会活动家。她与贝蒂·威廉斯一起创立了和平人社团组织,该组织致力于和平解决北爱尔兰问题。[8]1977年,两人同获1976年度的诺贝尔和平奖。[9]科里根还获得了一些其他奖项。
- Abrams, Irwin. The Nobel Peace Prize and the Laureates: An illustrated biographical history 1901–2001. USA: Science History Publications. 2001. ISBN 0881353884.
- Abrams, Irwin; Wang, Gungwu (编). The Iraq War and Its Consequences. Singapore: World Scientific. 2003. ISBN 9812385908.
- Buscher, Sarah and Ling, Bettina. Máiread Corrigan and Betty Williams: Making Peace in Northern Ireland. New York: The Feminist Press. 1999: 32 [2011-03-22]. ISBN 1558612009.
- Fairmichael, Rob. The Peace People Experience (PDF). Irish Network for Nonviolent Action, Training and Education. 1987.
- ^ 1.0 1.1 Fairmichael, p. 28: "Mairead Corrigan, now Mairead Maguire, married her former brother-in-law, Jackie Maguire, and they have two children of their own as well as three by Jackie's previous marriage to Ann Maguire."
- ^ Abrams (2001) p. 27 "For many years Mairead Corrigan (now Maguire), thirty-three when she received the 1976 prize in 1977, was the youngest in the year of the award, but she has now been matched by Rigoberta Menchú Tum, also thirty-three when she won the prize in 1992."
- ^ 3.0 3.1 Mairead Maguire: Nobel winner, veteran peace campaigner. AFP. 2010-06-04 [2011-02-05].
Maguire was born into a Catholic community in Belfast on January 27, 1944, the daughter of a window cleaner father and housewife mother, growing up with five sisters and two brothers.
- ^ 4.0 4.1 Mairead Corrigan Maguire. The Peace People. [2011-02-04]. (原始内容存档于2012年2月6日).
In September, 1981, Mairead married Jackie Maguire, widower of her sister Anne, who never recovered from the loss of her children and died in January, 1980. In addition to the remaining three children from the earlier marriage – Mark, Joanne and Marie Louise – Mairead and Jackie are the parents of John and Luke.
- ^ NORTHERN IRELAND: A People's Peace Prize. TIME. 1976-12-13 [2011-02-26].
To a standing ovation, Betty Williams, 33, and Mairead Corrigan, 32, co-founders of the Ulster Peace Movement (TIME, Sept. 6) arrived to accept the Norwegian People's Peace Prize.
- ^ Die Carl–von–Ossietzky–Medaille [The Carl von Ossietzky Medal]. Internationale Liga für Menschenrechte (International League for Human Rights). [2011-02-20] (德语).
- ^ Page, Glenn D.; Pim, Joam Evans (编). Global Nonkilling Leadership: First Forum Proceedings. India: Gandhi Media Centre. 2008: 31. ISBN 978-1-880309-11-7.
My love of reading especially the lives of the early Christian mystics, St. Francis and St. Clare, St. John of the Cross, St. Teresa of Avila, helped me in my Spiritual Journey. Later in life I was inspired by writings of Gandhi, Tolstoy, Dorothy Day, Thomas Merton, Thich Nhat Hanh, and John Dear. … I studied the Bible and found the 'Sermon on the Mount' and the life of Jesus an inspiring story of nonviolence, and came to agree with the late Fr. John McKenzie who once wrote 'you cannot read the gospels and not know that Jesus was totally nonviolent.'
- ^ Peace People – History. The Peace People. [2011-02-20]. (原始内容存档于2011-06-11).
This was the beginning of the Movement and the three co-founders worked to harness the energy and desire of many people in Northern Ireland for peace... Ciaran named the movement, Peace People, wrote the Declaration, and set out its rally programme, etc.
- ^ The Nobel Peace Prize 1976. Nobel Foundation. 2009 [2009-07-08].
The Nobel Peace Prize 1976 was awarded jointly to Betty Williams and Mairead Corrigan. Betty Williams and Mairead Corrigan received their Nobel Prize one year later, in 1977.
- 和平人社团组织
- 梅里德·科里根·麥奎爾,和平人社团组织
- International Fellowship of Reconciliation
- 诺贝尔和平奖得主梅里德·科里根,和平英雄
- 梅里德·科里根 – 简历,诺贝尔奖
- 爱尔兰诺贝尔奖得主,The Best Question
- Nobel Women's Initiative


text:
Wednesday, December 17, 2008
Gabe's Gifts
Once upon a time, CHERUBS used to be able to afford to send out new member packets and items for new and expectant parents. We haven't been able to afford to do so for the past few years unfortunately due to lack of donations and a membership that has grown wildly. But our New England members have started offering this service again to new and expectant members up north and we are thrilled and proud of what they are doing and hope to offer it across the U.S. again soon!
(from Corin)
Gabriel's Gifts is a project we started to provide new and expectant parents of CDH babies with gift bags. We all know how difficult it is to have a child with CDH living in the NICU fighting for life, and hope that these bags will bring a little comfort to those families.
In these bags we hope to include items such as a baby blanket/quilt, eye cover, hat, booties, journal, pens, disposable camera, childrens book, lip balm, CDH information packet, gift cards, and any other comfort items that may be helpful.
We are in desperate need of donations to put these bags together. For $20 you can sponsor a gift bag that will help a family in need. In exchange for your sponsorship we will include your cherubs story in the sponsored bag. We also would greatly appreciate in-kind donations of any of the above items. If you would like to donate some items or would like to sponsor a gift bag in honor/memory of your cherub please contact Corin at email@example.com.
No comments:
Subscribe to: Post Comments (Atom)


text:
The power of lasers unlocks an entirely new realm of skin care. With laser light energy, you can tame an incredible spectrum of facial concerns, ranging from scars to dark spots to wrinkles. The narrow laser beam allows for unparalleled precision that targets problem areas while leaving delicate, healthy skin unaffected. When you choose laser skin resurfacing, you're choosing a fresh start – and stunning skin that revitalizes your entire appearance.
The trick with laser skin resurfacing is finding the treatment option that's ideal for you. By offering two different laser treatments, Radiance Medspa caters to every type of patient.
We'll perform a digital complexion analysis to gain insight into what your skin really needs. Armed with this quantitative data, we will expertly approach your skin care goals. Many patients aim to treat:
During laser skin resurfacing, short pulses of light are sent into the skin. These repetitive beams break up damaged skin and hyperpigmentation, moving through one layer at a time. The epidermis is removed while the underlying dermis is heated. This not only removes unwanted discoloration and superficial flaws, but also triggers new collagen growth during healing. The end result? Smoother, firmer, younger-looking skin.
Fraxel is the heavy-duty option for patients seeking dramatic changes to their skin's surface. This laser will treat sun damage, wrinkles, and general signs of premature aging. Its microscopic laser columns penetrate the skin deeply but leaves healthy skin intact to help encourage more rapid healing. There is downtime involved, but it will be minimal, and allow you to get back to regular activities shortly.
One step down from Fraxel, this laser offers gentler results accompanied a simpler healing process. Ideal for younger patients who are hoping for subtle revitalization, Clear + Brilliant helps maintain their current skin tone and texture and put off aging. It's more intense than non-laser treatment like microdermabrasion, but less invasive than Fraxel.
Click on any of the above headings to learn more about these revitalizing treatments, and get in touch to learn whether they might lead to personal fulfillment.
Schedule a Consultation and receive a $50 coupon for your initial treatment package at Radiance!


text:
10 Unix Interview Questions You Should Be Ready For
Despite being one of the oldest operating systems around, with its roots going all the way back to the beginning of the '70s, UNIX is still very popular today. UNIX-based systems are widely used in environments that require maximum reliability and scalability, so don't be amazed if you come across a lot of job listings for positions that require UNIX knowledge. Whether we are talking about a position that implies just some basic knowledge of UNIX or one that requires you to be able to write code in your sleep, one thing is for sure: UNIX is here to stay, so if you haven't done it already, grab some books and start learning. Considering the fact that UNIX is based on the C programming language, and the two share a lot of common ground, an introductory course to C may prove to be a great starting point.
When you feel confident enough to make a move, put together an awesome resume and prepare for an interview for a job in this field. This article will walk you through the most common UNIX interview questions. Ready, set, GO!
Basic Theory Questions
1. What is UNIX?
It may sound as a trivial question, but you'd be amazed just how many people fail to give a clear definition, because simply stating that "Unix is an operating system" is not likely to impress the interviewer. The correct answer should look something like this:
UNIX is a multi-user multitasking-optimized operating system that can run on various hardware platforms.
2. How is UNIX different from Linux?
Linux is basically an open-source clone of UNIX so, while a lot of similarities between the two operating systems do exist, there are also a lot of differences. The main advantage of UNIX is that all the core components of the operating system come from the same vendor, which means greater overall stability and better software support from vendors. UNIX releases are more stable and consistent than Linux releases, making UNIX the better choice for enterprise use.
3. What is a kernel?
The kernel is the program that acts as a middle layer between software and hardware. When a program requires access to certain resources or processing power, the kernel is responsible for sending the correct signals to the CPU and managing all other running programs and services so that the resources are correctly allocated and no conflicts occur.
4. What is the difference between multi-user and multi-tasking?
Multi-tasking means that a user can run multiple tasks simultaneously on a single machine, whereas multi-user means that multiple users can operate simultaneously on a machine.
5. What is a UNIX shell?
A UNIX shell is an interface that acts as a command interpreter, translating user input into machine-understandable language and then passing it to the kernel for execution.
Once you ace the basic stuff, the interviewer is most likely to move on to some questions involving commands. Keep in mind that his role is not to see how good you are at memorizing commands, but rather how well you understand what those commands do and determine your ability to choose the right command in a certain situation. In order to successfully pass this part of the interview, make sure you formulate your answers as clear and as detailed as possible. You should consider taking a course to improve your communication skills prior to the interview.
UNIX Commands
1. What command can you use to display the first 3 lines of text from a file and how does it work?
There are two commands that can be used to complete this task:
head -3 test.txt – this uses the "head" command, along with the "-3" parameter that indicates the number of lines to be displayed;
sed '4,$ d' test.txt – this command uses the Sed text editor to perform the task. If the command was simply "sed test.txt" the whole file would have been displayed; however, in our example the delete parameter was used (d) to make Sed delete everything between the 4th and the last line (defined by the $ parameter), leaving only the first 3 lines of the file. It is important to mention that Sed does not actually delete the lines from the file itself, but just from the output result.
2. How can you remove the 7th line from a file?
The easiest way is by using the following command: sed -i '7 d' test.txt
Unlike the previous Sed command, this command also has the "-i" parameter, which tells Sed to make the change in the actual file.
3. What is piping?
Piping is a technique that consists of entering two or more consecutive commands, separated by the pipe symbol "|". Once the first command is executed, its output will be used as input for the second command, the output of the second command will be used as input for the third and so on, until the whole chain of commands is executed.
4. How do you reverse a string?
You can reverse a string by using a simple piping of two commands: echo "Mary" | rev
The first command will generate the output "Mary", which will become the input for the rev command, making it return the reverse: "yraM".
5. How can you find out what a command does?
You use man <command-name> in order to bring up the manual page that describes the actions of the specified command and any other additional options and parameters that command might have.
These are some of the UNIX questions interviewers usually ask during basic interviews. However, if the interview is for a more technical position, you can expect to encounter questions that are more difficult; still, there is no reason to panic, as UNIX itself was created to be quite logical, so a good knowledge of the basic commands and a bit of imagination can help you get the job done.
Check out this online course to learn how to use the UNIX command line to get the most out of OS X – yes, the OS X that runs on your Mac, which is UNIX based by the way. If this doesn't convince you that UNIX can be simple and intuitive, nothing else will.
Unix students also learn
Empower your team. Lead the industry.
Get a subscription to a library of online courses and digital learning tools for your organization with Udemy for Business.


text:
We've moved the libavg sourcecode from our own svn repository to github. Here's the project in it's full glory: https://github.com/libavg/libavg. Obviously, that gives us the full power of github, including much better issue and branch/merge tracking, easy forking, etc.. If you look at the libavg network graph, you can see that we're busy using all these new capabilities .
Most of the work needed – including moving all branches, issue tracking, adapting the continuous build, fixing web links and instructions etc. – was done by Richy, with help by OXullo and Benedikt Seidl. Thanks!
Also, we just released version 1.8.1, a bugfix release. Of course, we happily used github support for this.


text:
Fascism in Fergueson
The response I hear from many conservatives to the events in Fergueson bothers me. Not necessarily the fact that conservatives want to support law enforcement, since that's a standard conservative position, but how far they're taking their support for law enforcement, and the way they seem to be making this another partisan issue.
The conservative position on law enforcement makes sense based on their principles of respecting law and order. So I won't fault them for that at the moment. But surely there's a possibility of the police going too far–going beyond upholding the law to forcing their own will on others? In fact, conservatives frequently seem to assume that that's the case for federal law enforcement. Why does local law enforcement get a pass?
I mean, if the police started confiscating guns from concealed carry permit holders in the area of the protests, wouldn't conservatives go berserk? So why don't they care about violations of the First or Fifth Amendments, guaranteeing free speech, freedom of the press, and that no person will be deprived of life, liberty, or property without due process? Why aren't they troubled about journalists getting (reportedly) harassed and threatened by the police? Why aren't they concerned about the (reportedly) massively disproportionate force shown by the police against protestors and people who violate curfew? Why aren't they upset at the police for (reportedly) failing to protect businesses from looting while harassing and threatening people engaged in lawful protest?
It's not only liberals who suffer from an overly aggressive and excessively armed police force–a fact that conservatives understand at some level, because of their suspicion of federal law enforcement. By making this a partisan issue and showing lock-step support for the police in Fergueson, conservatives are hurting themselves as well. Conservatives and liberals should be able to stand together on issues of common interest, and a police state is, in principle, something that neither one of them is interested in.


text:
Kate Hudson And Matt Bellamy Are Over And She Might've Already Moved On To Derek Hough
Kate Hudson and Matt Bellamy of Muse got engaged in 2011 after a year of being together and three months later, she gave birth to a boy they named Bingham Hawn Bellamy (That will always sound like the name of a really pretentious law firm Connecticut). After months of rumors claiming that they are done with looking at each other's faces, Kate's rep tells People that they canceled their engagement a long time ago and they're no longer a thing, but they're going to remain the BEST of friends for the sake of their child and blah blah blah burp blah fart blah:
"Kate and Matt have been separated for some time now. Despite this, they remain very close friends and committed co-parents."
Kate was married to Chris Robinson for 7 years. Their son Ryder Robinson is 10.
Before Kate "settled down" with Matt and started raising Bingham with him, she put serious miles on her coochie's odometer by jumping on trick after trick. She was one of my slut icons. So since Kate is not the one to give her vagine a break, UsWeekly says that she's already getting on human Ken Doll Derek Hough. Kate and Derek have been friends for a while and a source tells UsWeekly that they "hooked up" this past weekend:
Sources tell Us that Hudson and Hough were together this past weekend at the Nice Guy Restaurant in L.A. One eyewitness tells Us that the stars ate dinner, drank, and stayed past closing.
Two things: I've eaten dinner, drank and stayed past closing at a restaurant (Side note: The employees at T.G.I. Friday's don't like it when you do that) with friends that were girls before and we didn't scissor afterward. So I don't know how Kate and Derek having dinner together proves that they're hooking up. Also, if Kate is dating Derek Hough, then I take back what I said about her not being the type to give her vagine a break . Because "dating" Derek Hough is one way of telling the world that you're giving your chocha some time off.


text:
Friday, August 22, 2014
2013 Azte's Corner Rose
Indian food seems to match rose better than any other wine I've tried yet. Friday nights, after a big week of work, seem to also pair quite well with Indian takeaway. So tonight I tried the 2013 Atze's Corner Rose along side a lamb rogan josh and chicken tikka masala, and it was great.
Opens up with a big wiff of strawberry and a touch of pineapple and rose water. Slightly sweet with fairly low acidity, which held up well with the Indian food. Grenache Roses have definitely found a spot on my wine rack.
Price: $15
Varietal: Grenache Rose
Tasted: August 2014
Rating: 90
Website: http://www.atzescornerwines.com.au/
Subscribe to: Post Comments (Atom)


text:
He shifted his weight in the third chair of the single pew at Baggage Claim C. He was amazed at how these thin seats initially looked comfortable, but soon made him squirm to find a good position. His elbows banged up against the metal arms on both sides of his seat. Why do humans intentionally design discomfort?
"Flight 0804 has arrived," the automated voice announced to the air. "Baggage can be claimed at Baggage Claim C. For passengers flying to another destination, please check the consoles located throughout the terminal."
He was expecting many important people arriving on this flight. Dignitaries, financiers, artists and musicians were listed on the manifest. But he was especially interested in a woman he had yet to meet. Her postcards always managed to reach him, bits of her life scribbled on the backs of beaches, mountains and cities. Her simplicity intrigued him.
"I like this town. I may stay here awhile."
"I didn't realize how much this beach meant to me."
"These mountains must reach heaven."
The tired-looking travelers started filing in one by one, pushing the turnstyle that ended their journeys. He recognized the young senator from Illinois who will lead a nation, and another man who will challenge Israel's sovereignty. He smiled fondly at a dark colored man who will sing about how wonderful life is, and nodded briefly at a man whose baseball career will be tainted by his credibility before lawmakers.
He stood when a small woman came through, carrying an overstuffed red purse and a slim computer bag. She seemed so . . . ordinary next to these other destinies. Yet he recognized her by her expectant glances at different faces, and a small smile that breathed anticipation.
She set her bags down near the baggage conveyer and ran her fingers through her hair. She was business-like in her movements, yet bent down to talk to a little Asian boy who will star in a TV series about being lost.
She saw him walking towards her, and her smile broadened. "I knew you'd be here," she murmured when he stood a few inches away.
He gently placed his hands on her shoulders, and said quietly, "Welcome to Gaia, child." They hugged as old friends do when time is of no essence.
"Let me help you with your bags," he offered, as they waited for the conveyer to deliver her life.
– a birthday present to myself


text:
|Project by mcoyfrog
|posted 09-12-2014 03:10 AM
|1332 views
|1 time favorited
|4 comments
Well I made these about 5-6 years ago along with about 20 others, these two didn't make the cut LOL have some checks from planer and cracks in wood. But last weekend I seen them sitting there and decided to finish them up so I sanded and oiled and figure I'll put them on craigslist to see what I can trade for ehh maybe I'll get something cool.
I'll let you all know what i get if anything
They are maple, yellowheart and I think bloodwood but maybe redheart can't remember. Maybe someone can tell me. I used bloodwood more then redheart back then. 7 1/2" x 12" rounded over sanded down and 3 coats cutting board oil.
thanks for looking
-- Wood and Glass they kick (well you know) Have a great day - Dug
4 comments so far
#1 posted 09-12-2014 05:24 AM
Nice looking cutting boards. I never thought to try yellowheart and maple, it's a great combo though. I like the size of them too, I have a couple big ones, but I find myself grabbing the small one most often.
I'm terrible at identifying wood, but I'm going to guess redheart. Again, I'm really not sure though.
It's fun to pull out older projects. I always like to see how I used to do things and compare to how I'm doing it now. Seeing your craft evolve is one of the best parts of what we do!
Thanks for sharing
-- I don't make mistakes, I make design challenges!
#2 posted 09-12-2014 02:24 PM
so true about the old projects, It was kinda cool because when I was out in the shop last night my son followed me out there, and now that he is a teenager he doesn't do that as much. So he was asking how to join the wood and how you made it seamless etc. It was a lot of fun to spend time when he was in a learning mode of my most favorite thing to do "spend time with him and talk about woodworking LOL"
Thanks for the compliment
-- Wood and Glass they kick (well you know) Have a great day - Dug
#3 posted 09-12-2014 09:33 PM
looking good
-- jack -- ...measure once, curse twice!
#4 posted 09-14-2014 11:49 PM
Thanks Jack
-- Wood and Glass they kick (well you know) Have a great day - Dug
Have your say...
You must be signed in to post the comments.


text:
1. No governmental relief is in sight for homeowners except in isolated instances of community action together with publicity from the media.
2. State and federal governments continue to sink deeper into debt, cutting social and necessary services while avoiding the elephant in the living room: the trillions of dollars owed and collectible in taxes, recording fees, filing fees, late fees, penalties, financial damages, punitive damages and interest due from the intermediary players on Wall Street who created trading "instruments" based upon conveyance of interests in real property located within state borders. The death grip of the lobby for the financial service industry is likely to continue thus making it impossible to resolve the housing crisis, the state budget crisis or the federal budget deficit.
3. Using taxpayer funds borrowed from foreign governments or created through quantitative easing, trillions of dollars have been paid, or provided in "credit lines" to intermediaries on the false premise that they own or control the mortgage backed securities that have defaulted. Foreclosures continue to hit new highs. Total money injected into the system exceeds 8 trillion dollars. Record profits announced by the financial services industry in which power is now more concentrated than before, making them the strongest influence in Federal and State capitals around the world.
4. Toxic Titles reveal unmarketable properties in and out of foreclosures with no relief in sight because nearly everyone is ignoring this basic problem that is a deal-breaker on every transfer of an interest in real property.
5. Evictions continue to hit new highs as Judges continue to be bombarded with ill-conceived motions that do not address the jurisdiction or authority of the court. The illegal evictions are based upon fraudulent conveyances procured through abuse of the foreclosure process and direct misrepresentations and fraud upon the court and recording system in each county as to the documents fabricated for purposes of foreclosure — creating the illusion of a proper paper trail.
6. 1.7 million new foreclosed properties are due to hit the market according to published statistics. Livinglies estimate the number to be at least 4 million.
7. Downward pressure on both price and marketability continues with no end in sight.
8. Unemployment continues to rise, albeit far more slowly than at the beginning of 2009. Unemployment, underemployment, employment drop-outs, absence of entry-level jobs, low statistics on new business starts, and former members of workforce (particularly men) are harbingers for continued decline in median income combined with higher expenses for key components, particularly health care. The ability to pay anything other than rent is continuing its decline.
9. Concurrent with the increase in foreclosures and the decrease in housing prices, official figures put the number of homes underwater at 25%. Livinglies estimates that when you look at three components not included in official statistics, the figure rises to more than 45%. The components are selling discounts, selling expenses, and continued delusional asking prices that will soon crash when sellers realize that past high prices were an illusion, not a market fluctuation.
10. The number of people walking from their homes is increasing daily, including people who are not behind in their mortgages. This is increasing the inventory of homes that are not officially included in the pipeline because they are not sufficiently advanced in the delinquency or foreclosure process. This is a hidden second wave of pressure on housing prices and marketability.
11. With the entire economy on government life-support that is not completely effective in preventing rises in homelessness and people requiring public assistance, the likelihood of severe social unrest and political upheaval increases month by month. Increasing risks of unrest prompted at least one Wall Street Bank to order enough firearms and ammunition to start an armory.
12. Modification of mortgages has been largely a sham.
13. Short-sales have been largely a sham.
14. Quiet titles in favor of homeowners are increasing at a slow pace as the sophistication of defenses improves on the side of financial services companies seeking free homes through foreclosures.
15. Legislative Intervention has been ineffective and indeed, misleading
16. Executive intervention has been virtually non-existent. The people who perpetrated this fraud not only have evaded prosecution, they maintain close relationships with the Obama administration.
17. Judicial intervention has been spotty and could be much better once people accept the complexity of securitization and the simplicity of STRATEGIES THAT WORK.
18. Legal profession , slow to start went from zero to 15 mph during 2009. Let's hope they get to 60 mph during 2010.
19. Accounting profession, which has thus far stayed out of the process is expected to jump in on several fronts, including closer scrutiny of the published financial statements of public companies and financial institutions and the cottage industry of examining loan documents for compliance issues and violations of Federal and State lending laws.
20. Prospects for actual economic recovery affecting the average citizen are dim. While there has been considerable improvement from the point of risk we had reached at the end of 2008, the new President and Congress have yet to address essential reforms on joblessness, regulation of financial services (including insurance businesses permitted to write commitments without sufficient assets in reserve to assure the payment of the risk. The economic indicators have been undermined by the intentional fraud perpetrated upon the world economic and financial system. Thus the official figures are further than ever from revealing the truth about about our current status. Without key acceptance of these anomalies it is inconceivable that the economy will, in reality, improve during 2010.
21. Real inflation affecting everyday Americans has already started to rise as credit markets become increasingly remote from the prospective borrowers. Hyperinflation remains a risk although most of us were off on the timing because we underestimated the tenacious grip the dollar had on world commerce. While this assisted us in moving toward a softer landing, the probability that the dollar will continue to fall is still very high, thus making certain non-dollar denominated commodities more valuable. This phenomenon could affect housing prices in an upward direction if the trend continues. However the higher dollar prices will be offset by the fact that the cheaper dollars are required in greater quantities to buy anything. Thus the home prices might rise from $125,000 to $150,000 but the price of a loaf of bread will also be higher by 20%.
22. GDP has been skewed away from including econometrics for actual work performed in the home unless money changes hands. Societal values have thus depreciated the value of child-rearing and stable homes. The results have been catastrophic in education, crime, technological innovation and policy making. While GDP figures are officially announced as moving higher, the country continues to move further into a depression. No actual increase in GDP has occurred for many years, unless the declining areas of the society are excluded from what is counted.
23. The stock market is vastly overvalued again based upon vaporous forward earnings estimates and completely arbitrary price earnings ratios used by analysts. The vapor created by a 1000% increase in money supply caused by deregulation of the private financial institutions together with the illusion of profits created by these institutions trading between themselves has resulted in an increase from 16% to 45% of GDP activity. This figure is impossible to be real. As long as it is accepted as real or even possible, public figures, appointed and elected will base policy decisions on the desires of what is currently seen as the main driver of the U.S. economy. The balance of wealth will continue to move toward the levels of revolutionary France or the American colonies.
24. Perceptible increases in savings and consumer resistance to retail impulse buying bodes well for the long-term prospects of the country. As the savings class becomes more savvy and more wealthy, they will, like their counterparts in the upper echelons of government commence exercising their power in the marketplace and in the voting booth.
2009-2010 livinglies recap2 Jan
1. No governmental relief is in sight for homeowners except in isolated instances of community action together with publicity from the media.
One Response to "2009-2010 livinglies recap"
[…] : EditComments : Leave a Comment » Tags: 2923.5, eviction, Foreclosure, Fraud, litigation, Mortgage modification, Predatory Lending […]


text:
All Is Well with our oldest Woody Boy
Hayden arrived home safe and sound, and thank the lord above, he was not affected by Norovirus as we were first told. It seems like it might of been the drinking water from a tank that made them really sick, or something to do with the food.
I got really panicky and upset, worried about our baby woody – he was suffering from a chest infection and did not need to be around anyone that was too sick. So I got Hubby to go to the shops and buy any cleaning products that would sterilize the house, and kept Hayden in a room by himself for 24 hours. Hubby even brought Wipes to wipe down the door handles/phone/fridge door or any where that Hayden touched for the period that he was in quarantine!!!
Luckily for Hayden he was fine within a few days, and double lucky for us none of the other WOODY BOYS came down with the bug. I still think that there might of been a dead animal in the tank water that made the water not fit for drinking. Or maybe the cook was sick of cooking, and wanted to get rid of the camp!!!!
We have had a few weeks of interesting times….
I have not been able to get online due to our move, and the fact that I could not connect with a land line in the house because the telecommunication company "sorry we made a mistake" and did not disconnect the line from the previous owners….so as the new owners of our current home we were not able to re-connect the line. Talk about a run around with the phone company…..It was only after that I threatened to take our business to some other company that anything was done.
So how our Woody boys coping with the biggest move of our married life?
Hayden – actually he is coping the best which really surprises me as he was so very against the move from day one.
Zachery – Man has that kid changed in the last week…..not sure where my sweet boy has gone to, but he has been replaced by one angry young man. So do not like the difference, and have been praying that he settles down in the next few weeks and goes back to being the sweet boy we know he is. I even took away his Harry Potter books because of the way he started to talk towards me….here is hoping for a better week ahead.
Nicholas – Surprise, Surprise…he loves his new school, wants to stay forever and has made some really good friends already. He is begging us to stay for next year so that he can do Year 7 at the same school and even take a trip to Canberra with his class mates. I pointed out that we will be going to Canberra any way with us traveling in a bus for a year!!!!
Cameron – Not sure how he is taking the new school, new house, new location. He worries me the most. Probably due to the fact the way his birth went down, and how we nearly lost him. He is very extra sensitive and I am hoping in time he will not feel so very nervous and make friends.
Kyle – my cheeky monkey has taken to the area, the house and has gone to school like he has always been there. He is so amazing….nothing has gotten to him. He keeps on smiling, gosh I hope that never ever changes !!!
Will update asap on how the move went down!!! THAT IS ONE VERY INTERESTING STORY, ONE THAT YOU WILL NOT BELIVE 🙂
Thank you to all of our friends that have sent us many wishes via text, and facebook…its much appreciated.
Looking forward to 2010 with many different experiences ahead for our family and our Wood Boys.
Lisa Wood
Filed under: So Much has Happened, Uncategorized | Tagged: All is Well, Biggest Move, Coping With Moving, Hayden Wood, Interesting times, Moving Changes, woody boys |


text:
OTTAWA — When her job as CEO of B.C.'s Children's and Women's Hospital was eliminated in 2002, Elisabeth Riley launched NavaHealth, the country's first private, patient navigation service.
The hospital executive, who now works in Ontario, was betting that many people — especially baby boomers — would be willing to pay $100 an hour to have a nurse accompany them to medical appointments as "another voice asking questions and another set of eyes and ears."
Word of mouth and articles in The Vancouver Sun and Canadian Business helped attract about 100 clients over a six-year period from 2002 to 2008. But Riley says it wasn't enough to sustain the business or even recoup her initial investment, the sum of which she would not disclose.
"When I started the business in 2002, it was a totally novel concept. The only place in Canada where patient navigation was going on was in Halifax," she says.
Nurse navigators have been helping cancer patients in the public system there since 2001.
"Nurses who are called case managers or patient care coordinators were a close concept, but they are inside the system and we were doing this from outside," says Riley, a physiotherapist by training who is now president and CEO of Orillia Soldiers Memorial Hospital in Ontario.
"Everyone I spoke to agreed it was needed, whether for people to have someone accompany them to medical appointments or those who needed home care and other help. In the health care system, knowledge is power, but you have to know how to get access and how to get your case prioritized. Everyone actually working in health care does it for colleagues and for friends."
NavaHealth had a virtual office and retained consultants, including a handful of nurses as patient navigators. They offered a "concierge service," she says, with an extensive variety of cases.
"We helped people find doctors, we helped get housekeepers and home care. We did medical literature reviews, we accompanied people to medical appointments and to hospital for their day surgeries. We connected patients to centres like the Cleveland Clinic and Mayo in the U.S. We did all kinds of cool things. We provided a really good service and it was very rewarding because we were helping people every day," Riley says.
"We had clients in Vancouver, Victoria, Kelowna, North and West Vancouver and White Rock. I thought it would be self-sustaining in a few years, but the problem in Canadian health care is that people aren't used to paying for anything."
In retrospect, Riley says the business might have been more viable if she'd managed to convince extended health benefits insurers to offer the services to members. She is uncertain whether Canadians will ever have an appetite to pay for private navigators.
In the U.S., where people are used to paying for health care, navigators are becoming more and more common — in both insured and non-insured settings and at for-profit and non-profit hospitals.
Private navigators charging up to $200 an hour help patients find the best doctors for their conditions and the best hospitals. They also research the best treatment options. Money can buy instantaneous access to surgeons, who will operate within days.
"But Americans are used to paying out of pocket for all kinds of things in health care," Riley says.
"At the end of the day, we all have to advocate for ourselves, but when you are lying there in a hospital bed, you're just an average Joe and that doesn't mean that you have the courage to say things to a nurse or a doctor like, 'Did you wash your hands?'
"We are totally vulnerable and at the mercy of others. So if you don't have a navigator, you have to have a family member be your advocate."
Sun Health Issues Reporter


text:
Stargazers Lounge Uses Cookies
Like most websites, SGL uses cookies in order to deliver a secure, personalised service, to provide social media functions and to analyse our traffic. Continued use of SGL indicates your acceptance of our cookie policy.
EQ5 And 130P-DS
Album created by
- 9
- 2
- 1
Images taken using my new 130P-DS and EQ5.


text:
One Good Thing by Jillee
Home Hacks, Cleaning Tips, & DIY
Header Right
All Homekeeping
Crafts & DIY Projects
Gardening & Outdoors
Natural Remedies
Essential Oils
Main Dishes
Side Dishes
Bright Ideas
All Bright Ideas
Saving Money
Green Living
Hair & Makeup
Log in
Instant Pot
If you know me, you know how much I love my Instant Pot! If you're looking to get more out of yours, then you've come to the right place! Here you'll find handy cooking guides, helpful how-to's, and recipes to save you time and money. Not sure where to start? Check out my
Instant Pot cooking time cheat sheet
, these 10 surprisingly
simple Instant Pot recipes
, or this list of all of my favorite
Instant Pot accessories
! For even more great recipes (including all of my
Instant Pot recipes
,) check out the
43 Of The Best Instant Pot Recipes To Make In 2020
Here Are The 21 Best Accessories For Your Instant Pot
5 Quick & Easy Freezer Meals That Will Save Your Sanity
5 Reasons Why This One Kitchen Gadget Is The Ultimate Money Saver
This Is The Most Brilliant And Useful Cleaning Hack For The Instant Pot
This Is What Scares People The Most About The Instant Pot [Video]
The One Cooking Guide You Need To Make Dinnertime Simple
How To Make Amazing Pot Roast In A Fraction Of The Time
How To Make The Best (And Easiest) Cheesecake Ever
11 Clever Hacks That Will Make You Love Your Instant Pot Even More
The 10 Things You Need To Know About Pressure Cooking Frozen Foods
The Easy Way To Make Perfect Hard Boiled Eggs Every Time
How To Make Delicious And Inexpensive Beans In Your Instant Pot
How To Make Gift-Worthy Vanilla Extract In The Instant Pot
Avoid These 9 Mistakes When You Use Your Instant Pot
These Quick And Easy Spare Ribs Are Hiding A Delicious Secret
This One Added Ingredient Makes The Best Rice Ever
This Is The Best Cheat Sheet For The Instant Pot, Period
The Best "Dump Dinner" You'll Ever Make
If You're New To The Instant Pot, Here's What You Need To Know
How To Deep Clean Your Instant Pot And Keep It Like New
8 Instant Pot Recipes That Will Make Thanksgiving Dinner So Easy
This Is The Comforting Navy Bean Soup That Mom Used To Make
This Is The Delicious Instant Pot Chili Recipe You Need This Fall
This Is The Easiest Way To Make Delicious And Healthy Homemade Yogurt
3 Easy Ways To Make Delicious Potatoes In An Instant Pot
Instant Pot vs. Slow Cooker – What You Need To Know
10 Surprising Things You Can Make In Your Instant Pot
How To Make 3 Easy "Dump Chicken" Recipes In Your Instant Pot
How To Make Fresh Strawberry Jam In Your Instant Pot
Go to page
Go to page
Next Page
Work With Us
Terms of Use
Privacy Policy


text:
Warm and natural family photography, capturing your unique lifestyle moments.
"Child and baby photography requires a light touch. Being a mother myself I understand how to work with little ones
so that they are happy, mum and dad are happy, and we can capture those spontaneous images that we all want."
My pricing starts at $375. for an average family shoot. Scroll down for package details.
Lifestyle Photography is a natural style that is gaining momentum – its kind of a photojournalist take on family life.
Its about photographing your family in your environment, capturing the little moments that are special to you.
Its a mix of action and fun, with posed family groupings too.
I find children get very tired during a shoot, so I allow extra time for breaks and snacks, nappy changes etc so everything
flows at a happy pace. We take things even slower with newborns. Photo shoots are demanding. I only book one
session a day to ensure I can bring all my focus and creativity to your session.
On receiving your booking, I will send you information on how to prepare
Consultation by phone
An Information PDF on how to prepare for your photo shoot
The Photo Session
1 photographer (me)
On-line Gallery for viewing
Editing time – RAW files to high res jpgs
Images in high res for print
Many photographs are taken on the day –a variety of poses and expressions, with changes of lenses and lighting.
I edit down to the very best and upload onto an On-line Gallery for you and your family to view before final purchase.
A 50% deposit is required to secure your booking.
If you are not 100% happy with your photos, we can re-shoot at no extra charge to you.
For babies under 2 weeks. Creating a beautiful styled portrait of baby, and natural portraits of mother/baby & parents/baby.
We take these shoots at a relaxed pace, allowing additional time for feeds and nappy changes.
For young families. A mix of natural family interactions and posed family portraits. Approx 1 hour shoot.
Ideas …. Feet stacks, teddy bear tea parties, dress-ups, beach and park shoots.
CLASSIC FAMILY PORTRAIT SESSION: from $375.
Posed family groupings – price is dependent on number of people and poses – eg Grandparents, Grandparents/Children.
Parents/ Children, Siblings portrait. Family Group Portrait. This is a perfect package for extended families.
Please email me for a quote.
Photoshop retouching – softening wrinkles, removing dark circles, brightening teeth etc
I use a combination of natural light, portable Studio lighting (see Gear List) and Speedlights to capture all the action.
The more professional the camera gear, the more beautiful the image. Shooting with the full frame Nikon D3s enables me
to capture nuances of light and subtle details in portraiture. Shooting in RAW captures all that information.
After your shoot, I choose a selection of the best images to go through a detailed editing process.
Choosing from the RAW files, the selected images are taken into Lightroom (a digital darkroom) where they are developed, adjusted for tonal range, colour balance, contrast and brightness. This is a time intensive process, but well worth it.
A RAW image is like a digital negative which needs to be developed to produce very high quality images.


text:
← The Kevin Eastman Interview Part I
This sequence from "The Losers" in Our Fighting Forces #161 (November 1975), written and penciled by Jack Kirby, inked and lettered by D. Bruce Berry. ©1975 DC Comics
Your email address will not be published. Required fields are marked *
Name *
Email *


text: Inside the Mind of a Villain by dgrinnell2345 | May 9, 2018 | Essays About Writing Get Free Email Updates!Signup now and receive an email once I publish new content. I agree to have my personal information transfered to MailChimp ( more information ) I will never give away, trade or sell your email address. You can unsubscribe at any time.


text:
ADM completes phase one of Al Zahiyah project in Abu Dhabi
Marks an important step in the completion of the overall Al Zahiyah project
Abu Dhabi Municipality has said that its department of urban planning and municipalities, in collaboration with strategic partners, including Musanada, has opened a vital street, thus marking the completion of Phase One of the development of the Al Zahiyah Project in Abu Dhabi.
According to a WAM report, works on the project started in early 2017 and was completed in March 2018, with the opening of the 10th Street, which extends 1,660m from Abu Dhabi Commercial Centre to Mina Street.
The completion of this project marks an important step in the completion of the overall Al Zahiyah project, as 10th street is considered a significant part of the entire development.
The report added that the opening of this phase of the road reflects the keenness of the Abu Dhabi City Municipality and its partners to meet the needs of road users and community members.
It added that the completion of the project was the result of the 'co-operation of all parties and illustrates their commitment to the corporate values aimed at improving the quality of life in the city, enhancing the urbanised outlook of Abu Dhabi city, and delivering an array of infrastructural projects'.
The Abu Dhabi City Municipality and its partners previously launched a project aimed at developing Al Zahiyah formerly known as the Tourist Club Area, said the WAM report.
It includes improving facilities and upgrading assets to achieve a host of objectives such as improving the traffic flow, carrying out street lighting, providing pedestrian paths, and others, it added.


text:
Welcome to the RoadBikeReview Holiday Gift Guide! In this economy, money is tight so you will want to be sure that you spend wisely. Our suggestions below are designed to save you time and let you know everything you need to about the different products on the market vying for your hard earned cash. Below is today's list of top rated picks, and be sure to head back to the RoadBikeReview 2011 Holiday Gift Guide main page to see the rest of the gift suggestions to help you this holiday season.
Cycling Computer and GPS gift ideas – A bike computer can be very helpful for cyclist, they can indicate speed, max speed, average speed, miles riden, and more are now recording your GPS location. With sites like Strava, you can even compete virtually with friends and other local cyclist for KOM and Sprint leader bragging rights.
We've put together a short list of computers you might be interested in for that lucky someone on your holiday shopping list this year. Ranging from the newest member of the Garmin Edge family, the 200 to a simple and easy to install Blackburn Atom 2.0.
Garmin Edge 200
|The Garmin Edge 200 is the least expensive in the class leading Garmin Edge lineup, but you wouldn't necessarily be able to tell from looking at it. It's housed in a similar sized casing as it's older brother the 500, and with it's glossy black exterior looks better in our opinion.
The similarities end there for the 200 though. The 200 lacks the wireless capability of the higher models, so you won't get heart rate, cadence, or power numbers. What you do get is a lower price of admission to get into a GPS capable computer, allowing you to log your miles on sites such as Strava. With a clear screen that displays what most casual riders care about, the combination of low cost and GPS should appeal to quite a few. Installation is a snap and can be moved from bike to bike easily. Also, from what we've gathered, battery life is excellent.
Key Features:
Website: https://www.garmin.com/
CatEye Urban Wireless
|The CatEye Urban Wireless computer is an easy to install computer aimed at anyone commuting or riding around town on their bike looking for all the basic functions you'd expect from a bike computer. It's got speed, time, and distance, operated by a single button. What makes the unit "urban" are a few specialized features like carbon offset and calorie consumption, information that will not only help you feel better from your ride, but better about your impact on the environment, too!
Key Features:
Website: https://www.cateye.com/
Blackburn Atom SL
|If you're looking for the best value in a bike computer, we'd suggest takinga look at the Blackburn Atom SL. It's got all the functions of it's older sibling the SL 3.0, but at a fraction of the cost. That's because the SL is wired. If the computer is destined to stay on a single bike, this should not be a problem. Though the unit is able to keep information from multiple bike setups, if you do choose to move from bike to bike. But at the low price of $20, it's a solid unit.
Key Features:
Website: https://www.blackburndesign.com/


text:
Freegate is one of the first anticensorship software intended to provide fast and secure internet access. By fast, it means that users get access to overseas websites as fast as to their local ones, and it doesn't require any installation or configuration, as it is an executable file. This client software works by logging-in a proxy network system: DynaWeb, a DIT's P2P-like server. Actually, this is the most used circumvention software in China, as it offers anonymity while being the last chance to access sites like facebook.com, twitter.com, youtube.com.
The interface is plain, but driven straight towards functionality. Blocked by ISP websites can also be viewed thanks to proxy server like technology, further enhanced by encryption and compression algorithms.


text:
International alarm has spiralled over the mounting toll, which rights groups suspect is partly the result of a government-backed shoot-to-kill policy.
The United Nations Commission on Human Rights added its voice to the condemnation on Wednesday, calling on officials to carry out their duty in accordance with international human rights standards.
The government and police have repeatedly denied any part in extra-judicial killings, arguing that most of the deaths have resulted from inter-gang warfare.
Thai Prime Minister Thaksin Shinawatra, who will have served half of his mandate on Thursday, again dismissed the allegations of a shoot-to-kill policy.
"They did not die because government officers killed them, they killed each other," he told reporters.
Thailand's interior ministry said on Wednesday that the high death toll meant that a blacklist of 46,177 people suspected of involvement in drugs had already been reduced by 20%.
Thai health officials estimate that three million Thais - roughly 5% of the population - are addicted to methamphetamines, and the government has set itself tough goals to rid the country of the drug.
Blacklist fears
Thailand's National Human Rights Commission said that around 50 people had complained that they had been mistakenly included on interior ministry and police blacklists.
Thirapat Assawasangsit, secretary to the commissioner who oversees drugs issues, said those blacklisted have been asked to report to police, but are afraid of what awaits them.
"They are reluctant to go because they think it would seem like they are admitting that they are involved. They feel very insecure now and they don't know which way to go, what to do next," he told the French news agency AFP.
The UNHCR special rapporteur on extrajudicial, summary or arbitrary executions, Asma Jahangir, asked the Thai authorities to ensure that "the strict limits on the use of lethal force... are followed rigorously and without exception".


text:
Hearts Afire is a 3 stage Parish-based Program from the Marian Fathers of the Immaculate Conception. It is designed to help us live a life of faith, charity, sacraments, set our hearts on fire with love of God and neighbor, and to inspire works of mercy in our families, parishes, and communities. It offers people a quick and easy way to withdraw from the demands of life and enter into a peaceful interior reflection on their relationship with Christ
Stage One: The Two Hearts (Parts A and B)
The purpose of stage one is to entrust ourselves to Mary, as Christ did at the Annunciation and as He willed His disciples to do at the foot of the cross.
Part A
We begin with Mary's Immaculate Heart, because Mary leads us to the Sacred Heart of Jesus. Starting with the book 33 Days to Morning Glory we prepare for Marian Consecration. This Do-It-Yourself "Retreat" consists of the book, 6 DVD's (30 mins long), and an optional Retreat Companion.
Part B
Here we encounter The Sacred Heart of Jesus. Using the book Consoling the Heart of Jesus we become aware of God's infinite Mercy and how we can give HIM joy! It teaches us to console His heart by accepting His love and trusting in Him completely. It also focuses on what to do with the suffering we all encounter. Consoling the Heart of Jesus consists of the book, 10 DVD's (45 mins long) and an optional Retreat Companion.
Stage Two: Wisdom and Works of Mercy
The purpose of Stage Two is to give group members a summary of Church teaching and Catholic wisdom. It suggests works of apostolate mercy, programs, and support. In stage two, we use the book The One Thing Is Three and an accompanying group-study forum. This stage is considered more of a group study, than personal retreat. Members will discern and choose an apostolate individually, as a group, or both. They will also be encouraged to help spread the message of Divine Mercy.
Stage Three: Keeping the Hearts Afire
This is the stage of ongoing formation. After having completed 33 Days to Morning Glory, Consoling the Heart of Jesus, and The One Thing is Three books, undertaken works of mercy, and being formally recognized by the Association of Marian Helpers, we encourage you to continue growing in faith. Perhaps attending AFF (Adult Faith Formation) offerings, parish renewal opportunities.(i.e Light of the World, Cursillo), or simply living with a sense of peace, mercy, love and forgiveness toward all those you encounter.


text:
1. From my last Miscellaneous Monday post, you know that we hosted Christmas in Chicago and then flew off to Denver for Allie's Wedding. Then I spent a weekend in San Francisco celebrating my friend Leah's 30th. Mr. Fix-It was back in Denver last week for work, and I also spent a couple of days at a training last week. We've also squeezed in about 100 other things - including Mr. Fix-It rewiring all of our lights. But that's a different post for a different day. I think we may have some normal time soon. I guarantee the giant pile of clothes beside my bed hopes so.
2. YOU GUYS - I mentioned this on twitter this weekend - I accomplished a BIG 30 before 30 goal! I shot a newborn session! It was such a great experience, and I want to shoot 100 more.
3. Speaking of photography, I spent Friday night updating my photography blog site and I designed a logo. No, I'm not changing careers, but I would feel SO fulfilled if I could shoot sessions here and there on the weekend.
4. So, just about a year ago, I posted about cleaning the wax off Hunter Boots. Guess what? They got all white and waxy again. Last time, I recommended tire spray (per a recommendation via google search). Know what actually works? This stuff. And, true story, it won't set off your carbon monoxide alarm.
5. While putting our Christmas stuff away, we decided to play with the layout of our living room. Not sure if it will be a permanent change, but it's fun to try something new!
What's new with you?


text:
You win if you're relaxedPar Arthole dans Recherches & Innovations le 8 Décembre 2004 à 15:11
You win if you're relaxed
October 29, 2004 10:26 AM
Still during Rebecca Allen's talk:
The MindGames group at MIT media lab Europe which develops technologies that can expand and improve human potential, they rely on signals coming directly from our bodies to interact with technology.
One of the Games is "Collective Calm", it observes the "Galvanic Skin Response", that phenomenon that happens when we are stressed or nervous, we start sweating and our body betrays that nervousness whether we want it or not.
Collective Calm is a biofeedback video game where you have to relax if you want to win.
Relaxation is measured via a handheld orb that measures each player's Galvanic Skin Response, which is then wirelessly transmitted to the game.
It's the exact opposite of many usual games that put players in a nervous state. Collective Calm also has the benefit to help players learn how they body react, responds, work to stimuli.
CommentairesAucun commentaire pour le moment
Suivre le flux RSS des commentaires
Ajouter un commentaire


text:
BOMA Announces New Officers
- By
- Jul 07, 2017
The Building Owners and Managers Association International elected its 2017 to 2018 officers at the 2017 BOMA International Conference & Expo in Nashville:
- Chair & Elected Officer: Robert Brierley of Colliers Boston
- Chair-Elect: Brian Cappelli of Global X Properties
- Vice Chair: Scott Jones of Jacobs
- Secretary & Treasurer: Keith Major of Bentall Kennedy (Canada)
BOMA Backgrounds
Brierley has nearly 30 years of commercial real estate experience and serves as managing director of Real Estate Management Services & executive vice president of Colliers Boston. As a two-term past president of BOMA/Boston, Brierley is also a member of BOMA/Boston's Board of Directors. He also served two consecutive terms on the BOMA International Executive Committee and as chair of the BOMA 360 Performance Program Council before becoming an officer of BOMA International in 2015.
He will have the full support of Colliers in the coming year as he represents the 25,000+ people that comprise the BOMA International organization," said Tom Hynes, CEO of Colliers, in prepared remarks.
Cappelli has more than two decades of experience in the industry and is currently vice president of Asset Management at Global X Properties. He served two terms as president of BOMA/Greater Cleveland and four years on BOMA Ohio's Board of Governors.
Jones currently serves as vice president for Jacobs and was an active member of BOMA/Fort Worth before moving to San Francisco. Jones was chair of both BOMA International's State Government Affairs Committee and the Careers in Real Estate Task Force Returning Veterans Outreach Sub-Group. He also has served two terms on the BOMA International Executive Committee.
Major is executive vice president of Real Estate Services with Bentall Kennedy in Toronto. Major is responsible for the property management, operations, leasing and development activities undertaken by the company for their Canadian office and industrial portfolio. He served on the BOMA International Executive Committee for four years and currently serves as vice chair of the International Council.
Image courtesy of Colliers International


text:
Here is a comment from The Cornish Democrat. A response from one of our group is below.
Kernow Action Now!
The Cornish have a long history with direct action (see C24 forum thread) not the least of which being the 1497 rebellion against an unfair taxation coupled with an English establishment encroaching on Cornish rights. Community groups and movements are to be found across Kernow perhaps the most high profile ones recently being the Trelawny Alliance and STIG.
Original Source: Kernow Action Now!
Shared via AddThis


text:
Veturi and A.R.Rahman have talents that are just more than mesmerizing. They have again and again proved their caliber by giving music that is out of this world. With lyrics that can squeeze your heart and with music and voice that can make any situation seem peaceful, they have come together many times and produced lovely songs. One such master piece is from the movie 'Sakhi' where the lyrics talk about the color and describe it so wonderfully that we are never over it.
Here are the lyrics and the video link.
kougili kougili kougili cheli pandu
ne ompe sompe tonikina toli pandu
pachamdaname pachadaname
toli toli valape pachadaname
pachika navvula pachadaname
yedaku sammatam chelime(2)
pachamdaname pachadaname
yedige paruvam pachadaname
ne chirunavvu pachadaname
yedaku sammatam chelime
kaliki chilakamma yerramukku
yerramukkule pilla vaakku
puvvai pusina yerra rojaa
puta gulabi pasi padam
yerrani rupam udike kopam(2)
sandhyaavarna mantralu vinte
yerrani panta padamante
kanchanala jilugu pacha
kondabanti goranta pacha
masake padite marakata varnam
andam chandam aligina varnam(sakhiyaa)
alale leni sagara varnam
moyile leni ambara varnam
mayura galame varnam
gummadi puvu toli varnam
udaa pu rekkalapai varnam
yenno cherenee kanne gaganam
nanne chere ee kanne bhuvanam
ratri nalupe rangu nalupe
vanaakalam mottam nalupe
kaki rekkallo karu nalupe
kanne katuka kallu nalupe
visigi pade koyila nalupe
nelaambarala kuntala nalupe(2)
tellani telupe yeda telipe
vanalu kadigina tume telipe(tellani)
irukanupapala kadha telipe
uduku manasu telipe
uruku manasu telipe


text:
100 days of fashion, Animal Print, black and white, business casual, fashion, fashion blogger, fashion challenge, Fashion inspiration, fashionblogger, Interview, leopard, Look of the week, Modern Day Fashion, ootd, sartorialist
I've been slacking on my posts lately, so I am going to make it up to you guys with a sudden rush of posts (stay tuned…).
So many people think that when going for job interviews you are confined to a look of slacks and blazers. While that certainly can be done, you can incorporate parts of your personality into your outfit.
Whether that is through animal printed scarves and shoes or an eye-catching tote bag, you can make a "drab" interview outfit look like something off the runway, while still looking professional and put together.
Scarf – Aldo
Ring – Forever21
Pencil Skirt – Vero Moda
Kitten Heels – Town Shoes
Keep an eye out for more posts in the coming days 😉