text:
LACONIA, NH (5/20/15) – On Saturday, May 30, Wyatt Park will celebrate its grand re-opening after over two years and approximately $75,000 of renovations.
The Wyatt Park Revitalization Project gained momentum in 2012 when HEAL NH awarded a $10,000 two-year grant to the City of Laconia to support healthy eating and active living in the Wyatt Park-South End neighborhood. The renovation plans for Wyatt Park and its immediate surroundings were developed in response to feedback from residents and business owners who voiced concerns about safety and access to the park.
Since the project began, there has been widespread support from community members and the City. While the Laconia Department of Public Works had a budget to repair a street in that area, the project was made a priority and an additional $15,000 was added to include extra safety and accessibility improvements, including ADA-compliant tipdowns for the crosswalks. The City Council allocated $50,000 for additional improvements in response to the widespread support for the project.
The combined funding allowed for several safety and accessibility improvements to Wyatt Park, while making the park more inviting for people of all ages, including:
- Water bubbler/bottle filling station in the park
- Bike rack and picnic tables
- New fencing and signage around the park
- Resurfaced basketball court painted for multiple uses including basketball, four square, hopscotch, and pickleball
- Lighting inside the park for safety
- Walking path installed around the park perimeter with extra green space
- High visibility signs, new crosswalks, sidewalks with tipdowns with detectable warning devices, and other pedestrian safety measures to improve safe access to the park.
The Wyatt Park Grand Re-Opening Ceremony will begin at 10am on Saturday, May 30, with the Civil Air Patrol Color Guard performing the National Anthem. A ribbon cutting ceremony with Laconia Mayor Ed Engler and other dignitaries will follow. Community members and the media are invited to attend. Rain date is Sunday, May 31. More information about the Wyatt Park Revitalization Project can be found on Facebook at http://www.facebook.com/WyattParkSouthEndCommunityRevitalizationProject.
About the Wyatt Park Revitalization Project:
The Wyatt Park Revitalization Project in Laconia was developed in 2012 as a result of a two-year HEAL NH grant awarded to the City of Laconia in collaboration with Lakes Region HEAL to support more choices for residents to eat healthy and be physically active. The Wyatt Park project was led by the Laconia Parks and Recreation Department in partnership with the United Way, the Laconia Police, Wyatt Park Association, Department of Public Works, Lakes Region Partnership for Public Health, and Lakes Region HEAL. Neighborhood residents were involved from the planning stages through execution, providing valuable input during community forums and the action plan development process.


text: 'If you want to do something about human trafficking, you have to talk about education and family education,' Iana Matei says [Stefan Matei/Al Jazeera] Pitesti, Romania - A new girl has just arrived. The 14-year-old is pale with long black hair pulled back in a ponytail. She is a victim of forced prostitution. "I see you had good grades for maths when you were still in school. That's great because we'll need to get you back to class," Iana Matei tells her. The girl smiles shyly.For 18 years now, the Romanian psychologist has been running a shelter for victims of sexual exploitation in a village near the city of Pitesti, about an hour's drive from Bucharest. During this time, she has helped hundreds of women and young girls who were trafficked into prostitution.Sometimes, she goes out and rescues them from their traffickers herself. It takes courage to do so because the perpetrators are often ruthless."I don't know if I am courageous, but I do know that I am angry," she says, sitting in her small, pink-walled office. There are photographs of girls who have been in her care on the shelves. Outside, in the garden, laundry is hanging to dry.The 57-year-old has a resolute air and lively blue eyes."Outrage - that's what drives me," she says. "I'm angry at the people who do this, who beat up a young girl, rape her and force her into prostitution so she'll be traumatised for life. And I'm angry at society for turning a blind eye. It's so unjust."Shelter for sex trafficking victimsRomania is the European Union country with the highest number of reported victims of human trafficking. The majority are women and girls trafficked into the sex industry. Destination countries include Italy, Spain and Germany, but also Romania itself. In recent years, the country has been attracting a growing number of sex tourists. And the victims are getting younger and younger, according to Iana."I am now setting up a shelter in the city of Cluj in the north. That area is terrible," she says. "You'll find nine-year-old girls working the streets."The traffickers put make-up on their faces and dress them up provocatively. But you can still see that they are very, very young. It's heartbreaking and nobody does anything about it."Iana opened the country's first specialised shelter for victims of sex trafficking in 1998, before the problem was widely known. RELATED: Going undercover as a sex worker At that time, she was in her home country for what she thought would be just a temporary visit. She had been living for years in Australia after fleeing Romania during the revolution against the communist dictator Nicolae Ceausescu."I had been demonstrating against Ceausescu on the University Square in Bucharest. When the police arrived, I ran for my life, and I lost my handbag with my ID," she explains. "So Romania was no longer a safe place for me. I fled to Serbia and eventually made it to Australia."In Australia, she studied psychology and set up an NGO to help street children. When, years later, she went back to Romania for a holiday, she asked about street children in Bucharest."Somebody said to me: 'Yes, we have street children here. They live in the sewerage under the city. The best thing to do would be to seal off the manhole covers and set the sewers on fire.' I was deeply shocked when I heard that."Returning to RomaniaBack in Australia, Iana couldn't shake that memory, so she decided to return to Romania for a year to work in orphanages and help the street children living in sewers. She was in one of those orphanages when, one day, the police called."They said: 'We have three prostitutes here. They've been working in Macedonia and are now back in Romania. Could you bring some clothes and a sandwich for them?'"When Iana arrived at the police station, she saw three terrified young girls, dressed in skimpy clothes and covered in bruises. One had cigarette burns on her thighs."The girls were 14, 15 and 16 years old. I said to the police: 'These are children, and they are obviously abused.' They [the police] just laughed in my face; to them, they were just bad girls who liked sex."One of the girls told Iana that she had run away from her trafficker. She begged not to be put out on the street again, afraid that her trafficker would find and kill her. "She had run away for the second time. The first time she went to the police in the town where she was exploited and was immediately brought her back to her trafficker. She got beaten up badly, but she had the guts to run away again."She begged me to help her. I realised I really needed to find these girls a safe place to stay. But the problem was, there were no shelters."So she called child protection. "One of their people came to the police station and, in the presence of the girls, he said: 'I am sorry, but I cannot put these whores in an orphanage because they will set a bad example for the other children.'"During a medical examination, all three girls were found to have a venereal disease and had to spend a few days in hospital. That gave Iana a little more time to work out how she could best help them. "I decided I would take care of them myself," she says. "But to do this in a legal way, I had to have an NGO. So I set up the paperwork for that.Because I didn't have space at home, I rented a flat where I could shelter the girls. I decided to live with them, and that was a good thing because this way I learned a lot about the needs victims have."It was a tough decision and meant giving up her comfortable life in Australia."I also had a teenage son who had agreed to only come for a year with me to Romania so I could work with street children," she says."But in the end, he understood that these girls didn't have anyone to take care of them and he was willing to share me with them."'Her ear was cut off with scissors'While Iana is talking, the girl who just arrived has settled on the sofa and is already chatting with the other girls - all teenagers. One is cuddling her baby. The shelter is a simple country house. Upstairs are bedrooms, downstairs a living room with a television and drawings of trees on the wall. Inside are also Iana's office and a workshop with sewing machines and jewelry made by the girls that are displayed, hanging on a board."It's important that they learn crafts," Iana says. "It will keep them busy - keep them from thinking too much. "These girls have been through hell, you know. They have been battered, forced to sleep with 20, 30 men per day. They've been deprived of food, they've been tortured. I've had a girl here whose ear was cut off with a pair of scissors."'A girl, you can sell a thousand times'When Iana set up her first, improvised shelter, human trafficking was exploding in Eastern Europe as a consequence of the wars in the Balkans. "Human traffickers had the networks of arms smugglers and drug traffickers. But selling humans proved to be much more profitable," she says. "You can sell a gun only once, but a girl, you can sell her a thousand times. Depending on how long she lasts, of course."New victims kept coming to her. With money she obtained from a charity, she was able to hire social workers. "In a short time we ended up with 12 girls in a three-room flat so we had to rent another one," she says.Rescuing girls from their traffickersSometimes, victims would tell her about other girls who were in the same situation. Often they had their telephone numbers and Iana would manage to contact the girls. "And then we would work out a plan to rescue them," she says."The girl in our care would call the victim first and tell her she's in a safe place. Then I would talk to the girl and find out as many details about her situation as I could, to see when there would be a possibility for me to come and 'kidnap' her."We always plan these rescue operations carefully," she says. "But I don't want to give you too much information because I want to save more girls in the future."Asked about the danger of these operations, Iana smiles broadly: "It's dangerous because anything can happen. But, on the other hand, the traffickers don't expect you to challenge them. They know everyone is afraid of them, so they're not vigilant."Whenever I kidnap one of their girls, they are in shock. And they always think I am a 'madam' who steals their girls to exploit them in her own business. Some of the girls even think I am a madam initially. They are so used to people exploiting them. They can't believe someone would want to help them."All they have known is 'violence and abuse'Nowadays, Iana is considered an expert on human trafficking. Her organisation, called Reaching Out Romania, shelters up to 12 girls in the centre near Pitesti. In the same area, there is a 'transit' shelter for six girls who continue their education. Then there's the centre in Cluj that she is setting up.She and her staff see to it that the victims go back to school and are provided with psychological counselling. They also work with the girls on repairing their self-esteem and acquiring life skills."With each girl we set up an individual plan," she explains. "It is important that they find out what it is they like doing. I push them to go to school because a lack of schooling is one of the main reasons why they end up on the street. These girls often have absolutely no life skills; they don't know how to do anything."Their story is always the same: They come from extremely dysfunctional families where they have suffered all kinds of violence and emotional abuse."This makes them vulnerable to trafficking, she explains. "All they have heard during their childhood is: You are stupid, you are good for nothing, no one wants you. So when some guy comes around and tells them he loves them, they are swept off their feet. They will do anything for him. No matter if he beats them. They are used to being beaten. But the difference is that this man says he loves them."At one point, the man will say: 'Let's go to Spain. I will work in construction and you will work as a babysitter. We'll save the money, and when we get back, we will buy a house.' She believes him, but of course once she is in Spain, she is forced into prostitution." 'Sold by their own parents'According to Iana, there are organised gangs who traffick girls, but there are also individual pimps and even family businesses. "I have seen many girls that were sold by their own parents."She lights a cigarette. From her office you can hear girls laughing in the living room."The only way to stop trafficking is through education and through addressing dysfunctional families," she says. "But the hardest thing is to change the mentality of the people."In the village, my girls are called names. Old women are gossiping that we make porn films in the shelter. "Once a girl has been in prostitution, the people here don't want to have anything to do with her. No matter if the girl was forced or if she is underage - she's just a filthy prostitute, they'll say. But how on earth can you call an abused 13-year-old girl a prostitute?" SOURCE: Al Jazeera ABOUT THE AUTHOR Renate van der Zee Human Rights

subset: commoncrawl


text:
The US Internal Revenue Service has recently been compelled to release to the public its field service advices. Doug Harrell finds them to be full of useful accounting information.
Field service advices (FSAs) are memoranda prepared by various officeswithin the office of chief counsel at the national office of the Internal Revenue Service (IRS). Their purpose is to provide non-binding advice, guidance and analysis to IRS agents, attorneys and appeals officers to help them develop an issue or determine litigation hazards regarding both substantive and procedural issues.
In FSA 1999-15004, an IRS agent sought advice from the national office on a captive arrangement that seemed to satisfy many of the standards enunciated by a number of court cases. The national office advised the agent to gather additional information and to develop more detailed factual circumstances for its analysis of whether to litigate the deductibility of the premiums paid to the captive. The requested information appears to focus on the possible sham or non-commercial aspects of the captive arrangement under review. The instructions given to the agent provide an insight into the way the IRS will evaluate a captive insurance arrangement. The following is a summary of the instructions given to the IRS field agent:
• Parental and/or brother-sister guarantees: The existence of parental and/or brother-sister guarantees, hold harmless agreements, letters of credit, etc. should be determined. The IRS contends that the existence of brother-sister guarantees, hold harmless agreement, letters of credit, etc. would indicate that the requisite risk shifting has not occurred. It has advised agents to review files with the state as a source for such data.
• Capitalisation: Compare the capitalisation of the captive with the amount of risk insured and review the captive insurer's premium to surplus ratio. The agent may also consult an actuary regarding whether the capitalisation is indeed adequate. If (it is) not adequate, perhaps there has not been a real risk transfer.
• Regulatory issues: Examine the regulation of insurance in the jurisdiction to determine if there is a strict regulator or a weak regulator that would permit significant under-capitalisation of the captive, or permit a parent to legally borrow out nearly all of the captive's available cash. If the regulation is minimal, agents should determine if either the parent or the captive takes advantage of the latitude allowed by the regulator.
• Unavailability of reasonably priced insurance in the marketplace:
Information should be gathered with regard to the business reason for the formation of the captive insurer. Facts regarding the insurance coverage for the controlled group prior to the formation of the captive should be developed. What was the parent doing to handle risks prior to the existence of the captive? Is the individual who is managing the captive the same person who was initially the risk manager running the parent's self-insurance reserve? If so, an analogy can be made that nothing has really changed.
The IRS national office also asked the agent to examine contracts, reinsurance, financial ratios, ownership, and business relationships and any documents that would suggest the captive is a sham.
From the foregoing, it is possible to draw the following conclusions:
• FSA 1999-15004 gives taxpayers some insight into additional areas of IRS interest and concern in its analysis of captive insurance arrangements. The IRS has not retreated from its "economic family" position set forth in Revenue Ruling 77-316 and Revenue Ruling 88-72.
The IRS still maintains that the rulings are the guiding authority on the deductibility of captive insurance premiums, despite the reluctance of many courts to accept its analysis. However, the IRS seems to have acknowledged to its agents that its "economic family" theory is not widely applied or supported in the courts and is asking them to develop other lines of attack.
As taxpayers continue to form captive insurance companies as a means to help them manage their risks and to obtain insurance coverage, they should be cognisant of the thinking of the IRS. To reduce the likelihood of having to face a challenge to the deductibility of the premiums paid to the captive, every effort should be made to satisfy or respond to the factors outlined in FSA 1999-15004.
Douglas Harrell is a senior manager with KPMG and heads up its US tax practice in the Cayman Islands. He has been with KPMG for 11 years, and has worked extensively in the area of US taxation of offshore captive insurance companies for the past seven years.


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VERNON - The concept of preserving a transportation corridor on the railway tracks between Vernon and Kelowna is picking up steam.
The District of Coldstream is looking at options to keep the line—which shut down in July and hasn't reopened—intact for transportation, even if the mode isn't rail.
"Our first priority is to see if we can keep it as a rail line," Coldstream mayor Jim Garlick says. "If not our second idea is a transportation corridor for the future."
After Kelowna Pacific Railway went bankrupt in July, Canadian National picked up operation of 75 per cent of the network. The condition of the track between Vernon and Kelowna was poor, and CN felt it couldn't justify repairs. That's led to backlash from Okanagan businesses who shipped large portions of their product by freight. Ashland Chemicals plant manager Kelly Brown is hoping a third party will come forward to operate the line, but so far, there haven't been any takers.
According to a Vernon advocacy group called the Okanagan Rail Trail Initiative, the Central and North Okanagan have been given a choice and an opportunity in the event that rail service does not resume. Society creator Brad Clements has been visiting municipal governments across the Okanagan, urging them to take action before the corridor is lost forever. Clements believes a rail trail would boost tourism and have wide ranging benefits on health, transportation and environmental preservation. CN has already started its process of selling off the line, with first right of refusal being offered to the federal government Dec. 3. If neither federal, provincial or municipal governments purchase the land, it will be offered to adjacent property owners.
"I think there is a real opportunity to have something good come out of this," Garlick says. "I hope everyone (federal, provincial, and municipal governments as well as the public) can work together. If we miss this opportunity and it's all sold off that would be a shame."
In a media release, Kelowna mayor Walter Gray said council remains hopeful that a business proposal to resume freight service will be submitted by the Dec. 2 deadline. If not, he said the city would continue investigating the possibility of a rail trail. But because the line is a private business on a regional line that traverses three municipal boundaries, he said "the City of Kelowna's control of matters is limited."
Meanwhile, Coldstream is looking into zoning the section of rail that goes through its district specifically as a transportation corridor. It's already designated as such in Coldstream's official community plan.
For some North Okanagan politicians, it's all too familiar to the closure of the Okanagan Valley Railway's Armstrong to Sicamous line in 2009, and the failed efforts to establish a rail trail on it afterwards. Regional District of North Okanagan director Mike Macnabb has said he supports the idea of a rail trail, however he's hesitant to climb aboard. Garlick thinks it's different this time.
"There's a larger population affected by it, we have larger centres involved," he says. "We have two ministers plus the premier. I think we can rally some support if need be."
Okanagan mayors are scheduled to meet Dec. 11 to discuss options for the railway.
To contact the reporter for this story, email Charlotte Helston at email@example.com, call (250)309-5230 or tweet @charhelston.


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Rustic Cake Smash
When thunderstorms rain on your scheduled 1st birthday cake smash and your smile is turned upside down, all you can do is have a slice of cake and hope for sunnier days ahead. That's exactly what Canberra delivered the morning of Rhys re-scheduled rustic cake smash.
There's something so magical about this woodsy location. It makes the perfect backdrop for our Rustic Cake Smash set-up with "Mike" the resident gentle giant kangaroo popping in to say hello.
Rhys grinned from ear to ear the entire session. He clearly enjoyed his yummy giant cupcake compliments of The Sugary Sinners Cupcakes. It's safe to say Rhys enjoyed the the entire sensory experience including his relaxing bath to wash it all off.
Happy 1st Birthday Rhys!
If you're looking for a fun way to mark your baby's first birthday we'd love to create something beautiful for you. Contact us


text:
By Leslie
. . . and afternoon. We have had simply glorious weather – balmy temperatures, sunny days, and just a trace of wind. These are the days the Lord has given us – beautiful. And we've earned them, after five long months of extremely hot weather.
But yesterday afternoon was even more special – I got to lie outside for the first time on my new custom wheelchair. Oh to inhale the outdoors, smell the air laden with the scent of flowers, herbs and trees. And, also to "supervise" the planting my husband was doing to replace the now faded summer blooms.
When one is denied the simple pleasures – of sitting, standing, walking – the "work-arounds" become ever so much more meaningful. I am ever so grateful to my husband, Bill, who designed and pushed forward the construction of my special chariot. Because of his work and never giving up, I can look forward to many more "beautiful mornings" . . . . and afternoons.
Come join me.
November 2014


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Bombshells at Sony
- 10,000 jobs and R&D funding to be slashed
- US$520 Billion loss posted last financial year
- Hints that Sony may ditch TV manufacturing as Apple enters market
- Looking to compete on smart phones with Samsung and HTC
KAZUO Hirai came out swinging.
Swinging the axe.
Sony's new chief executive, who took over from Welsh-born Sir Howard Stringer this month, said he would cut 10,000 jobs and slash research and development spending on everything but mobile devices, cameras and gaming consoles.
He may even, he hinted, ditch the company's historic television manufacturing business.
Given the glacial pace of decision-making at the Japanese electronics giant, these were bombshells. Whether they will satisfy shareholders is a different question.
Analysts noted that despite the blizzard of revenue targets, profit goals and restructuring promises in Mr Hirai's coming out presentation yesterday, the company's operational future remains thinly sketched, and difficult.
Meanwhile, Apple's entry to the TV market looms, and it probably won't be good news for incumbents.
What is for sure is that Sony is about to post its biggest-ever loss, which it estimated earlier this week at US$520 billion for the financial year just ended.
Spiralling costs in consumer electronics and disappointing sales, notably of its Bravia flat-screen TVs, mean Sir Howard's promise of a 5 per cent operating margin was as elusive at the end of his reign as it was throughout. Mr Hirai promised it again yesterday.
"We have heard a multitude of investor voices calling for change," he told a packed news conference at Sony's Tokyo headquarters, close to the company's first factory established 65 years ago.
"Sony will change. I will definitely change Sony and revive it.
"Sony has always been an entrepreneurial company. That spirit has not changed... We can't turn away from making painful decisions, because if we are scared of the pain, we won't be able to change."
Mr Hirai was elevated to the top job a year before Sir Howard had been expected to retire, thanks to his success at the helm of Sony's computer and video games division.
The son of a wealthy banker, Mr Hirai was well travelled even before going into business, and his stint working for Sony in New York means he is a keen internationalist. Nonetheless, he is Japanese, and investors hope this will make it easier for him to impose reform on a conservative corporation that is a pillar of the country's establishment.
Ben Keen, the chief analyst at technology research firm Screen Digest, said Mr Hirai's slimming plan is overdue.
"The obvious and most interesting contrast is Sony versus Apple and the biggest difference that Apple exhibits is this: focus. Apple is incredibly focused. It will only work on a very few products and it will only bring a very few to market. Sony is a very broad company and it has tried to do too much, tried to cover too many bases. It needs to narrow its products down, narrow down its research and development effort and pick some key products."
Sony must look across at Apple and wince. Sony invented the personal music player in the Eighties, when every teenager seemed to have a Walkman, but then Steve Jobs came along and unveiled the iPod and the Japanese firm has been playing catch-up ever since.
By the time Sony had cracked digital music players, Mr Jobs was finalising his revolutionary iPhone.
Mr Hirai promised that, now it has taken control of its smartphone business that used be run as a separate joint venture with Ericsson, there will be much closer integration of the company's phones, tablets and Vaio laptops.
The question is whether it can differentiate itself from a host of new competitors, from South Korea's Samsung to HTC of China, who have reputations for cutting-edge tech, have cheaper labour costs and aren't hobbled by a strong currency, as Sony is by the yen.
When he set out his priorities for Sony yesterday, what was not on the list was more telling than what was. Investment will be concentrated in three areas: mobile, gaming and "imaging", which covers cameras and video cameras.
Televisions were not on that list of "core" businesses, and Mr Hirai came close to saying the division must "shape up or ship out".
Asked what he would do if the TV business does not return to profitability by 2014, after what would then be a decade of losses, Mr Hirai said: "We naturally need to consider what to do when the business turns profitable as we planned and what to do otherwise, not to mention we also need to consider courses of action to take in each case."
The company has cut 66,500 jobs in four restructuring plans since 1999, but it still had 168,200 employees as of March 2011, according to data compiled by Bloomberg. The bulk of the new cuts will fall in the TV division, but analysts pointed out that the headline figure is not as dramatic as it may first appear.
The 10,000 figure includes previously announced measures, such as the spin-off of a chemicals business that accounts for 3,000 jobs.
Sony's axe-wielding new boss is only two weeks into the job, of course. Sony shares barely moved yesterday. Investors will wait to see how heavy the axe will really fall.


text:
School of Medicine
Showing 1 of 1 Results
KC Huang
Professor of Bioengineering and of Microbiology and Immunology
Current Research and Scholarly Interests How do cells determine their shape and grow?
How do molecules inside cells get to the right place at the right time?
Our group tries to answer these questions using a systems biology approach, in which we integrate interacting networks of protein and lipids with the physical forces determined by the spatial geometry of the cell. We use theoretical and computational techniques to make predictions that we can verify experimentally using synthetic, chemical, or genetic perturbations.


text:
Alayna larayneBBW pleasurePosted: Tue. Jul. 28 04:09:29 2020
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$200/1 hour
Gender: Female
Height: 162 cm
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Bust: DD Cup
Dress: BBW
Ethnicity: not set
Availability: Escort, Incall
Location: Whitiora
- Location: Hamilton
- Age: 25
- Phone: 0276458968


text:
This was published 14 years ago
Assisting the police with their inquiries …
The Australian Federal Police faces further political heat over its role in tipping off the Indonesians about the activities of the Australians who became known as the Bali Nine.
The national police force's guidelines allow it to share information about criminal suspects with foreign police forces until charges are laid, even if the crimes under investigation could lead to the death penalty.
After charges have been laid, the Attorney-General, Philip Ruddock, has a role in deciding whether information should be shared with foreign police in death penalty cases.
But in countries where the legal system allows for suspects to be detained without charge, deciding whether to share information can be a moot point if a person is charged just before the trial, when all evidence has been gathered.
In the Bali Nine case, the AFP commissioner, Mick Keelty, has faced a barrage of questions over whether the Australians should have told the Indonesians about the investigation, or simply kept watching and arrested the suspects on their return.
In response, Keelty has insisted that Australia needs to work with other countries in this way if it is to fight drug smuggling and terrorism effectively.
Now there are claims the Australian force failed to advise one of the accused he was under investigation before he travelled to Indonesia, which might have stopped him going.
The anti-death penalty network co-ordinator for Amnesty International Australia, Tim Goodwin, says there is "a great deal of disquiet in the community that we're a country that doesn't have the death penalty and stands for opposition to the death penalty, and yet, in practice, information would be used in this way".
Goodwin says the case has exposed a policy gap in the way the force operates. In sharing information that can lead to the death penalty being imposed overseas, it runs counter to other Government bodies. "In a number of other areas there are clear lines that we're not going to co-operate when it comes to executions."
In extradition cases, the Australian Government negotiates assurances from a country that it will not apply the death penalty in that case, even if that would otherwise be the local penalty.
When an Australian is sentenced to death overseas, the Government's practice is to make representations at the highest level in that country - sometimes through the Prime Minister, John Howard - with the hope of having the sentence commuted to imprisonment.
Goodwin expects the Bali Nine trials to raise this question again, particularly if evidence supplied by Australian police is used in court. "[In the Schapelle Corby case, the political pressure] started off fairly small but the issue started to grow when she was facing the death penalty," he says.
A former Australian ambassador to Indonesia, Rawdon Dalrymple, doesn't see that Keelty had much choice in the Bali Nine case, as several of the accused were not boarding flights to Australia.
"If it's in our national interest to stop these people from bringing substantial quantities of heroin in, then I think we just have to co-operate with other police forces," he says.
But the political pressure could get intense if the nine Australians are convicted and sentenced to death.
Says Goodwin: "We haven't seen that happen in Australia in many years. It remains to be seen how people will react if that happens again."
But Dalrymple thinks there will be little backlash. "I can't imagine there will be as much public sympathy for these people as there was for Corby, but on the other hand she wasn't facing the death penalty."


text:
Selection of NSK Folk Art artefacts at the Taipei Biennial 10
by nskstate.com on Sep.14, 2010, under Exhibitions
A selection of NSK Folk Art artefacts, curated by IRWIN and NSKSTATE.COM
at the Taipei Biennial 10, Sept. 7 – Nov. 14, 2010
Presented artists: Peter Blase, Christian Chrobok, Danaja, Dape, Haris Hararis/NSKSTATE.COM, David K, Charles Krafft, Goran Lišnjić, Lili Anamarija No, Christain Nnoruga, NSK.IS, Avi Pitchon, Alex Ross & Chris Wade, Armin Zerbe
The internet
was one of the key tolls for spreading the NSK State. In 1994 the NSK Electronic Embassy Tokyo website was launched which was active for quite some time and then in 2001 Haris Hararis from Athens launched NSKSTATE.COM which was maintained together with other NSK citizens and became the central meeting point for them. This was a crucial and unpredicted move and through this website citizens began to self organize.
We also discovered wide range of activities and artifacts related to the NSK and NSK State at various occasions and from time to time these authors would inform us about their works. It looked as if NSK citizens and others had begun to use the conceptual space of the NSK State in Time and the iconography of it and of various NSK groups in their own ways. Our decision was not to
restrict such initiatives, but on the contrary to support them.
Here are some examples:
On the East Coast of the USA, a film maker Christian Matzke opened an NSK library on his own initiative. The library has opening hours and a whole set of tools for theoretical readings.
In Iceland, NSK citizens have organized, again on their own
initiative, the NSK Guard of Iceland project and opened their own temporary NSK Embassy in Reykjavik.
Charlie Krafft from Seattle designed and produced sets of porcelain plates for NSK Embassies in mid 1990s.
In 2008 Irwin and NSKSTATE.COM began to collect so-called NSK Folk Art artifacts and decided to exhibit them.
As Alexei Monroe a London based theoretician and NSK citizen stated in his text NSK: The State which Ran Away with Itself for Irwin's upcoming book:
"Although it has only become apparent gradually, NSK's inherent, catalytic improbability has always been active behind the scenes, determining both NSK's actions and, crucially, people's reactions to it. Even now that NSK is far-better known and has several thousand citizens, it continues to generate unexpected responses emerging from the collective NSK space inhabited by fans and citizens, some of which have been even more surprising to the artists themselves. What we are dealing with here is a series of uncoordinated initiatives and responses, all inspired by the aesthetics or the concepts of NSK"
The artifacts shown in the frame of 10 Taipei Biennial were selected
by Hongjohn Lin and Tirdad Zoldhar from the range of NSK Folk Art works registered by Irwin and NSKSTATE.COM, based on the Volk Art concept first outlined by Alexei Monroe.
Irwin was present at the previous Taipei Biennial with the project NSK Passport Holders. At that occasion video interviews were shown based on research into the massive surge in applications for NSK passports from Nigerian citizens in the years 2007-2008.


text:
Teach English in Language Center in Luogang, Guang
Short about the positoin
About the position
Our training center in Luogang district, Guangzhou is looking for suitable foreign English teachers to join our dynamic team to start immediately. The position information includes the following.
Job Description:
* Teaching English;
* You are required to teach students in a training center;
* The students are aged from 3-15 years old;
* The base salary is 12000 RMB per month before tax;
* Accommodation is provided;
Working Schedule:
* Work 5 days per week with 2 days off, including 25 teaching hours per week plus 2-3 office hours;
* Native English speakers preferred;
* Accept non-native English speakers with perfect English accent;
* With teaching experience preferred;
* Good language teaching ability;
Review of applications will begin immediately and will continue until the position is filled. Candidates should submit a cover letter, Resume/CV, recent photo to: email@example.com
Salary: 12000 CNY(RMB) /Month
Housing: Provided
Contract type: Full time
Work visa provided: No
Teaching Students age: Training center (Children)
Work Location: Luogang, Guangzhou
Working hours: Work 5 days per week with 2 days off, including 25 teaching hours per week plus 2-3 office hours
Positions available: 2
- Teaching experience
- Accepted age range: 21 to 55
- British, American, Canadian, Australian, New Zealand, Irish, South African, European, Russian, Colombian, Portuguese, Other Ukrainian or Polish Passport holders
Apply online via this link:
Alternatively, send your CV, passport copy, certificates copy and photo to:
Email: email@example.com Or for more details call: (China) +86(0)20-37303381 Or (USA) +1 312-273-1868, Skype: findworkabroad3
Contact info


text:
Trinamool leader's daughter kidnapped at gunpoint
Published: 17th September 2016 11:22 PM | Last Updated: 17th September 2016 11:22 PM | A+A A-
BARASAT: The teenage daughter of a Trinamool Congress leader and councillor of Basirhat Municipality in North 24 Parganas district was kidnapped at gunpoint today, police said.
The 15-year-old girl had gone to her sister's place at Gajalmari in Basirhat, from where four motorcycle-borne men abducted her at gunpoint, the police said quoting the FIR lodged on the complaint by the victim's family.
Her father Laltu Mondal is a Trinamool Congress leader and the councillor of Ward number two of the municipality. Police have started raiding various places in search of the girl.


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There's a new release from Ben Butler & Mousepad on Musique Large and here is the video to one of its tracks. It was directed by Joe Howe, who is one of half of the project alongside Bastian Hagedorn.
You can pick the 12-inch of the Gif'N'Run EP at Boomkat or Juno, the digital release should be available soon on iTunes and other stores.
Lastly, please lend your ears to Ben Butler & Mousepad's contribution to the La Mixette series if you haven't already.


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Gordon McAlpine's adorable books follow the misadventures of 12 year old identical twins Edgar and Allan Poe.
The Tell-Tale Start is the first book in the series. The story revolves around the twins' quest to get back their kidnapped black cat, Roderick Usher.
Once Upon a Midnight Eerie is the second book in the series. Someone is trying to kill the twins, who are playing Poe in New Orleans.
The third book in the series, "The Pet and the Pendulum" will come out in April 2015. The book is set in an old mansion right outside Baltimore.
Visit Gordon McAlpine's webpage for more info on the author and the series.


text:
Friday, 27 April 2018
Peacock Colours...
23 comments:
Hi JohnReplyDelete
Great card.Enjoy your holiday Jan.
Hi John, a great card, love the blue panels with the feathers. Have a great day everyone. BxReplyDelete
Lovely card today.ReplyDelete
This looks so effective. One to try.ReplyDelete
Hi JohnReplyDelete
A great card.
B xx
Hi John, Lovely card, love your blues. Hope it's hot where you are, very concrete sky here. Have fun. SueL xReplyDelete
This is definite my kinda card I love CAS cards Ideal for posting tooReplyDelete
I hope you're relaxing
Gorgeous card, John.ReplyDelete
Anne (Northampton)
Morning John, wow, wow, wow this is gorgeous.ReplyDelete
HI know it is the first full day for you and Sam so enjoy yourselves and relax. Is it hot where you are.
Stay safe, healthy and happy, hugs, June Smith xxx
Hi John, great card, love the new stamps. Hope and Sam you are now both enjoying your holiday.ReplyDelete
Lovely card. Enjoy the holiday. Take carexxxReplyDelete
- Anonymous27 April 2018 at 12:31
Hi John, I saw this in your demo on TV and thought what a nice card it made, interest but not too fussy. Have a great holiday, love from Jackie xxReplyDelete
Hello John,ReplyDelete
Fabulous, I love this today.
Have a great holiday and totally relax (if your brain will let you!).
love Maureen x
Hi John,ReplyDelete
A lovely card.
Hope you are relaxing.
Hi John, Hope you and Sam have a great holiday and I really do hope you switch off for a day or two at least.This card is really effective and the colours are super.Have a good time,stay healthy and happy,Lots of Love Barbara D xxxReplyDelete
Hi John,ReplyDelete
a really brilliant card and such a super design too.
The colours and stamping look amazing.
Hope you are enjoying your break.
Lots of crafty regards. Jenny L.
Hi John, beautifully designed card, hope you have a super holiday, Kate xReplyDelete
Hi John this is a stunning card I love it. Have a wonderful time. We are having lots of rain here. Take care. Hugs JackieReplyDelete
Hi John great card gorgeous colours Hope you having a worderful holidayReplyDelete
Gorgeous card John love it.ReplyDelete
Hope your enjoying your holiday
Hug's Lynda xx
Hi JohnReplyDelete
What a great card, beautiful stamps and wonderful colours. Hope you are having a lovely holiday and have got some sun.
Love Diane x
Great CAS card John.ReplyDelete
Enjoy your holiday.
Gorgeous card John. My media plates etc., came yesterday so hopefully will find time to try them out today. Enjoy your holiday. Dark and overcast here. Take carexxxReplyDelete


text:
We've received a decent amount of emails asking us for high-end television recommendations. We understand that this deal isn't for everyone, but if you're in the market for a HDTV and have a healthy shopping budget, we've found a really hot deal.
NewEgg is offering the
Samsung UN46C7000 46" 3D 1080p 240Hz LED-Edgelit LCD HDTV Bundle for $1544.20 with Free Shipping when you use coupon code EMCZZNN54 during checkout! sold out!
This is an absolutely fantastic deal for those looking for a cutting-edge HDTV package. Edgelit LED backlighting allows for the TV to be only 1" thin! The UN46C7000 comes equipped with a variety of Samsung Apps including Youtube, Blockbuster, Amazon Video-On-Demand, Pandora, NetFlix, Skype and Facebook! The TV also features 4 HDMI, 2 USB and 1 Ethernet inputs.
The bundle includes the Samsung TV (reg $1900) along with the Samsung BD-C5900 3D Blu-ray Player (reg $205) and a Shrek 3D Starter Kit (2 pairs of Samsung Active Shutter 3D glasses and the Shrek 3D Movie - reg $308). If purchased individually, this bundle would cost over $2400! To get the deal, follow these steps:
- Add Samsung UN46C7000 46" 3D LED HDTV to cart at $1899
- Shrek 3D Starter Kit and Samsung BD-C5900 3D Blu-ray Player will be added to cart automatically for FREE
- Enter promo code EMCZZNN54 to bring your total price down to $1,519.20 w/ Free Shipping!
If you already have a Blu-ray player and are only looking for a 3D TV, TigerDirect has the Samsung LN46C750 46" 3D 1080p 240Hz LCD HDTV for $1099.99 when you use coupon code OWY11812 during checkout!
This TV is very similar to the Samsung 3D TV being sold at NewEgg, except that this one is not an LED-backlit TV. The LN46C750 features a 1920x1080 (1080p) resolution, 240Hz refresh rate and 4 HDMI inputs.
For the $450 price difference, it's probably a better investment to go with the NewEgg bundle, but if you are only interested in the TV, the TigerDirect is still a good deal.
Thanks for Visiting and Tell Your Friends!


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SHANGRI-LA APPOINTS DIGLBi AS GLOBAL AGENCY
Leading Asian hospitality brand taking digitally-driven, innovative approach to marketing as it seeks to evolve at the pace of the consumer and connect across the customer journey
Hong Kong, 24 March 2016 – Shangri-La Hotels and Resorts has appointed a fully-integrated agency team, led by global marketing and technology agency DigitasLBi, as Shangri-La's agency of record. DigitasLBi will lead the relationship with support from advertising agency BBH and media agency Zenith.
The integrated team, all part of Publicis Groupe, will work on brand strategy, creative strategy and execution, digital platforms, social media, customer relationship management, and media planning and buying across global markets for the Shangri-La, Hotel Jen, Kerry Hotels and Golden Circle brands. The move to a digitally-focused agency team is aligned with Shangri-La's data-led marketing approach, which is placing digital front-and-centre, and will drive efficiencies across its marketing and media spend.
"The way consumers connect and engage with brands has and continues to evolve at a rapid pace. Consumers are in a constant state of evaluation and use multiple channels and devices to research and purchase travel," said Shangri-La International Hotel Management Limited Chief Marketing Officer Steven Taylor, who joined the group in January 2015. "Fifty per cent of total travel sales will involve more than one device in 2016 and consumers are spending more time and visiting more sites when researching travel online. Brands today need to be able to look across the entire customer journey in all its complexity, and obtain a holistic view of how consumers connect and be able to respond to ongoing conversations around the brand at a moment's notice," said Taylor.
Following Shangri-La's investment in a new CRM platform last year, the group is in the process of redesigning its entire branded digital presence to deliver a responsive, authentic and personalised guest experience.
"We have been inspired by Shangri-La's vision of engaging a new kind of global traveller and their uniquely human approach to hospitality. Today's customers decide what luxury means to them and expect brands to recognize their individual preferences and behaviours, which is why we're excited to partner with Shangri-La to develop a data-driven approach we hope will help them to lead and innovate in a highly competitive environment. We are joined at the hip with BBH and Zenith and together will move the Shangri-La, Hotel Jen, Kerry Hotels and Golden Circle brands forward," said DigitasLBi Global Client Service Director Laurent Ezekiel.
DigitasLBi's work for Shangri-La will be underpinned by behavioural data, which will enable Shangri-La to understand what truly matters to its customers and to use these insights to gain competitive advantage.
Prior to consolidating its agency relationship, Shangri-La had been working with multiple agencies. DigitasLBi was retained last year to develop and execute the fifth anniversary campaign for Golden Circle, the loyalty programme for the Shangri-La family of brands.
"We loved DigitasLBi's creative solution and were impressed by their deep understanding of our audience. The 'Loyalty Is' Golden Circle campaign fostered a global conversation about loyalty and what true loyalty means to our guests and our employees," said Taylor. "With their global scale and integrated approach, we felt DigitasLBi was the ideal partner to build customer engagement and loyalty as we bring our brand promise to life across every part of the guest experience," said Taylor.
"This fully-integrated agency team model reflects Publicis Groupe's continued drive to break down agency silos in order to create more effective partnerships with clients," said Ezekiel.
Hong Kong-based Shangri-La Hotels and Resorts (www.shangri-la.com) currently owns and/or manages 97 hotels under the Shangri-La, Hotel Jen, Kerry and Traders brands with a room inventory of over 39,000. Shangri-La hotels are five-star deluxe properties featuring extensive luxury facilities and services. Shangri-La hotels are located in Australia, Canada, mainland China, Fiji, France, Hong Kong, India, Indonesia, Japan, Malaysia, Maldives, Mauritius, Mongolia, Myanmar, Philippines, Qatar, Singapore, Sultanate of Oman, Taiwan, Thailand, Turkey, the United Arab Emirates and the United Kingdom. The group has a substantial development pipeline with upcoming projects in mainland China, Cambodia, Hong Kong, India, Myanmar, Philippines, Saudi Arabia and Sri Lanka.
DigitasLBi exists to make brands matter to people. We offer forward thinking clients an ambitious, confident and collaborative community of experts including 450 data scientists and madtech advisors, inspiring 2000 creative minds, across 40 offices and in 26 countries around the world. Our work spans integrated communications, platforms, apps and media as well as commerce, mobile and innovation services, across clients of all shapes and sizes, defined only by the fact that they share our enthusiasm for the new opportunities that data and technology offer for creative work.
# # #
PRESS CONTACT: Lori Lincoln
Director of Corporate Communications
Shangri-La Hotels and Resorts
Tel: (852) 2599 3323
E-mail: email@example.com
Website: www.shangri-la.com
For digitised pictures of the group's hotels, please go to www.shangri-la.com/imagelibrary


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A previous bioinformatics-based search for small RNAs in Escherichia coli identified a novel RNA named IS183. The gene encoding this small RNA is located between and on the opposite strand of genes encoding two transcriptional regulators of the acid response, gadX (yhiX) and gadW (yhiW). Given that IS183 is encoded in the gad gene cluster and because of its role in regulating acid response genes reported here, this RNA has been renamed GadY. We show that GadY exists in three forms, a long form consisting of 105 nucleotides and two processed forms, consisting of 90 and 59 nucleotides. The expression of this small RNA is highly induced during stationary phase in a manner that is dependent on the alternative sigma factor σS. Overexpression of the three GadY RNA forms resulted in increased levels of the mRNA encoding the GadX transcriptional activator, which in turn caused increased levels of the GadA and GadB glutamate decarboxylases. A promoter mutation which abolished gadY expression resulted in a reduction in the amount of gadX mRNA during stationary phase. The gadY gene was shown to overlap the 3′ end of the gadX gene, and this overlap region was found to be necessary for the GadY-dependent accumulation of gadX mRNA. We suggest that during stationary phase, GadY forms base pairs with the 3′-untranslated region of the gadX mRNA and confers increased stability, allowing for gadX mRNA accumulation and the increased expression of downstream acid resistance genes.
One of the main Escherichia coli defenses against extreme acid is based on the induction of two glutamate decarboxylase enzymes, GadA and GadB (5). These two enzymes use glutamate as a substrate to catalyze the production of γ-aminobutyric acid, a process that consumes an intracellular proton (10). The pool of glutamate is believed to be renewed by the GadC protein, which is a putative amino acid antiporter that exchanges γ-aminobutyric acid for external glutamate. The expression of gadA, gadB, gadC, and other genes of the glutamate-dependent acid response has been found to be extensively regulated. GadE, a LuxR family regulator, is essential for inducing gad genes in E. coli (12). Additionally, a poorly understood interplay between two opposing AraC-like regulators (GadX and GadW) contributes significantly to the regulation of expression of the two decarboxylase enzymes (13, 14). GadX transcriptionally activates GadA and GadB expression, and some studies suggest that GadW acts as a repressor to oppose this action (24, 27). Additional reported regulators of the gad system include the stationary-phase sigma factor σs, the cyclic AMP receptor protein CRP, and the nucleoid protein H-NS (13, 27).
Recent studies have led to the identification of a large number of small regulatory RNAs in E. coli (3, 6, 28). Many of these small RNAs still have uncharacterized functions. Among the small RNAs that have been studied, most bind to the Hfq protein and function by base pairing with target mRNAs (reviewed in reference 26). The Hfq binding small RNAs characterized thus far have been shown to function by three different mechanisms. The DsrA and RprA RNAs activate translation of the alternative sigma factor σS (15, 16). These small RNAs form base pairs with the 5′ leader sequence of the rpoS mRNA and inhibit the formation of a secondary structure that normally occludes the ribosome binding site (16, 17). The OxyS RNA is representative of a group of small RNAs that repress the translation of target mRNAs. The OxyS RNA forms base pairs with the fhlA mRNA across the ribosome binding site, thus blocking ribosome binding and translation (1, 2). A third mechanism used by small RNAs to control gene expression is the destabilization of mRNAs. The RyhB RNA forms base pairs with specific mRNAs encoding proteins involved in iron metabolism and targets them for degradation by RNase E (18, 19).
Here we show that a novel small RNA identified as IS183 in a screen for conserved promoter and terminator sequences controls the expression of genes involved in the glutamate-dependent acid response. This regulatory effect is mediated by positive regulation of the gadX mRNA, which encodes a transcriptional activator of the acid response system. Due to its location within the gad gene cluster and its role in regulating acid response genes, IS183 has been renamed GadY. The gadY gene sequence overlaps the 3′ end of the gadX mRNA encoded on the opposite strand. Since these sequences were found to be necessary for the GadY-dependent accumulation of gadX mRNA, we suggest that the base pairing between GadY and gadX in the overlapping region results in stabilization of the gadX mRNA transcript. This is the first example of a regulatory RNA that positively regulates the accumulation of its mRNA target.
Plasmids and bacterial strains.Standard molecular biology procedures were used for the isolation of genomic DNAs and plasmids, for restriction digests, for molecular cloning, and for transformation by electroporation or heat shock. Platinum Taq DNA polymerase (Invitrogen, Carlsbad, Calif.) was routinely used to amplify DNA fragments. The sequences of all fragments generated by PCR and all mutations generated by site-directed mutagenesis were confirmed by sequencing. All plasmids and bacterial strains used for this study are listed in Table 1. The sequences of all primers used for this study are given at the following web site: http://dir2.nichd.nih.gov/nichd/cbmb/segr/segrPublications.html .
MG1655 was used as the E. coli parent strain for all strains unless otherwise designated. The rpoS::Tn10 (from GS015 [11]) and ΔgadX::kan (from EK426 [14]) alleles were moved into MG1655 by P1 transduction (25) to generate GSO108 and GSO110, respectively. Deletion of the gadY gene was carried out by the method reported by Datsenko and Wanner (9), using plasmid pKD4 (kanamycin resistant) and primers JK114 and JK115, to create strain GSO111. Disruption of the gadY promoter was carried out by the method reported by Court et al. (8), using the primer GadY-promoter, to create strain GSO109.
To allow for the overexpression of RNAs without additional sequence being added onto the 5′ end in the cloning process, we introduced an EcoRI site adjacent to the transcription initiation site of pKK177-3 by site-directed mutagenesis using primers JK17 and JK18 and a Quick Change mutagenesis kit (Stratagene, La Jolla, Calif.), generating plasmid pRI. For the overexpression of gadY, the appropriate sequences were amplified from MG1655 genomic DNA by the use of primers JK116 and JK118. For the overexpression of the antisense sequence of gadY, the appropriate sequences were amplified from MG1655 genomic DNA by the use of primers JK119 and JK120. The PCR fragments were then digested with EcoRI and HindIII and cloned into the corresponding sites of pRI to create plasmids pRI-GadY and pRI-YdaG. For the construction of plasmid placZ-gadXgadY−10mutant, the lac promoter was PCR amplified from MG1655 genomic DNA by the use of primers lacZ-S2 and lacZ-A1. The gadX gene was PCR amplified from strain GSO109 genomic DNA by the use of primers GadX-S5 and GadX-A2. A lac promoter-gadX gene hybrid was then created by a PCR with primers lacZ-S3 and GadX-DN-Nest3 and was cloned into the BamHI and HindIII restriction sites of plasmid pACYC184. For the construction of plasmid placZ-gadXΔgadY, the lac promoter was PCR amplified from MG1655 genomic DNA by the use of primers lacZ-S2 and lacZ-A1. The gadX gene lacking its 3′ untranslated region (3′ UTR) was PCR amplified from GSO111 genomic DNA by the use of primers GadX-S5 and GadX-A2. A hybrid DNA containing the lac promoter and the gadX gene lacking its 3′ UTR was then created by a PCR with primers lacZ-S3 and GadX-DN-Nest3 and was cloned into the BamHI and HindIII restriction sites of plasmid pACYC184.
Growth conditions.The strains were grown at 37°C in Luria-Bertani (LB) medium. Ampicillin (100 μg/ml), tetracycline (10 μg/ml), kanamycin (30 μg/ml), or chloramphenicol (30 μg/ml) was added when appropriate. Cells were harvested at the indicated times after 1:100 dilutions of overnight cultures. The typical optical densities at 600 nm for 1:5 dilutions of cells grown for 1, 2, 4, and 6 h were 0.06, 0.2, 0.7, and 0.9, respectively.
Northern analysis.Total RNAs were isolated from cells at various stages of growth, as specified in the figure legends, by using Trizol reagent (Invitrogen) according to the manufacturer's protocols. For detection of the GadY small RNA, total RNAs (5 μg) were fractionated in 8% polyacrylamide-8 M urea gels and transferred to Zeta Probe GT membranes (Bio-Rad Laboratories, Hercules, Calif.). The JK138 oligonucleotide probe was 5′ end labeled with 32P by the use of T4 polynucleotide kinase (New England Biolabs, Beverly, Mass.). The membranes were hybridized in Ultrahyb Oligo buffer (Ambion, Austin, Tex.) at 45°C and washed with 0.2× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) plus 0.1% sodium dodecyl sulfate (SDS). For the detection of mRNA species, total RNAs were fractionated in a 1% agarose-0.05 M MOPS-1 mM EDTA-3.3% formaldehyde gel and transferred to Zeta Probe GT membranes (Bio-Rad). The membranes were hybridized in Ultrahyb Oligo buffer (Ambion) at 45°C and then washed with 0.2× SSC plus 0.1% SDS. GadX mRNA was detected with the oligonucleotide probe GadX-A1. gadA mRNA was detected with the oligonucleotide probe GadAB-A1.
Primer extension analysis.RNA samples (5 μg) were incubated with 0.5 pmol of a 5′-end-labeled primer specific for GadY (IS183-A4) for 5 min at 95°C and then cooled to 42°C. After the addition of deoxynucleoside triphosphates (a 1 mM concentration of each) and avian myeloblastosis virus reverse transcriptase (10 U; Life Sciences Inc., St. Petersburg, Fla.), the reactions were incubated for 1 h at 42°C. The cDNA products were fractionated in 8% polyacrylamide-urea gels.
3′ RACE analysis.We performed 3′ rapid amplification of cDNA ends (RACE) assays as described previously (3). RNAs were ligated to the RNA adapter E1. Primer extension was carried out with primer E4. Amplification of the GadY cDNA was performed with Taq DNA polymerase and primers E4 and JK108. Amplification of the gadX cDNA was performed with Taq DNA polymerase, primer E4, and either primer GadX-S1 or primer GadX-S2. Amplified cDNA fragments were cloned into pCR2.1 TOPO and then sequenced.
Immunoprecipitation.Cell extracts were prepared from cultures of strain MC4100 or GS081 that had grown for 16 h. Immunoprecipitations were performed as described previously (30), with 20 μl of Hfq antiserum or preimmune serum, 24 mg of protein A-Sepharose (Amersham Biosciences), and 200 μl of cell extract.
Protein identification.Overnight cultures were diluted 1:100 and grown for 2 h. Aliquots were centrifuged, resuspended in 150 μl of a solution containing 4 mM Tris (pH 8.0), 30 mM KCl, 0.2 mM MgCl2, 1 mM dithiothreitol, and 0.2 mM phenylmethylsulfonyl fluoride, and sonicated for 10 s. Laemmli buffer (40 μl) was added, the samples were boiled at 95°C for 5 min, and 10 μl of each sample was analyzed by SDS-10% polyacrylamide gel electrophoresis. Bands of interest were excised and digested in the gel with trypsin (Roche, Indianapolis, Ind.) (1 μg/protein band) as described at the web site http://www.abrf.org/ResearchGroups/ProteinIdentification/EPosters/pirgprotocol.html . Peptide masses were determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF), and proteins were identified with Protein Prospector software (http://prospector.ucsf.edu/ ).
A 105-nucleotide small RNA encoded between gadX (yhiX) and gadW (yhiW).A small RNA named IS183 was previously identified in a genome-wide computational screen for novel small RNA genes in E. coli (6). The gene was predicted based on the presence of conserved σ70 −35 and −10 promoter sequences followed by a potential Rho-independent transcription terminator. Two RNAs, of approximately 100 and 90 nucleotides, were reported to be detected by Northern analysis (6). This small RNA is encoded in an intergenic region between two open reading frames, gadX (yhiX) and gadW (yhiW), which code for transcriptional regulators of the glutamate-dependent acid response in E. coli (Fig. 1A) (14, 27). Due to the location of the IS183 gene in the middle of the gad gene cluster and the IS183 role in regulating acid response genes (see below), this small RNA has been renamed GadY. A sequence analysis showed that GadY is only present in strains that also encode gadX and gadW. Thus, the small RNA gene can be found in all sequenced strains of E. coli as well as Shigella, but not in other closely related organisms such as Salmonella and Klebsiella.
We first sought to characterize the GadY RNA in terms of its size. To confirm the initial identification of this RNA, we performed a Northern analysis, using an oligonucleotide probe capable of hybridizing 30 nucleotides upstream of the predicted Rho-independent terminator. Three species of the GadY small RNA, of approximately 100, 90, and 60 nucleotides, were detected in this analysis (Fig. 1B). We determined the boundaries of each of these RNA species by carrying out primer extension and 3′ RACE assays. Primer extension analysis identified three alternative 5′ ends of GadY, which are located at nucleotides 3662494, 3662509, and 3662540 of the E. coli genome sequence (Fig. 1C). 3′ RACE identified a single 3′ nucleotide located at nucleotide 3662598 of the E. coli genome sequence, which constitutes the 3′ end of the previously predicted Rho-independent terminator (data not shown). Thus, the three GadY RNA species are 105, 90, and 59 nucleotides in length and from here on will be referred to as GadY(105), GadY(90), and GadY(59) (Fig. 1C). The predicted σ70 −10 promoter sequence (TATATT) is located immediately upstream of the start of GadY(105) (Fig. 1D). GadY(90) and GadY(59) are believed to be processed forms of the full-length 105-nucleotide transcript due to the fact that the expression of all three forms was eliminated when we mutated the promoter of the full-length RNA (see below). The sequence at the 5′ end of both GadY(90) and GadY(59) is UUU, and both 5′ ends are adjacent to predicted stem-loop structures, but it is not known what features of the RNA direct processing.
rpoS-dependent expression of GadY.The expression profile of GadY was examined under different growth conditions. An overnight culture of wild-type E. coli cells was diluted 1:100 into fresh LB medium and incubated aerobically at 37°C. The total cellular RNA was harvested at various time points after inoculation, and Northern analysis was performed on equal amounts of RNA to determine the GadY RNA levels at each point during growth. The GadY RNA levels remained low during the first 2 h of growth, after which the amount of the small RNA increased dramatically. GadY RNA levels peaked after 6 h of growth and then slowly declined (Fig. 2A). The sharp increase in GadY expression corresponded with the entry of the E. coli cells into stationary phase. Interestingly, we observed a differential regulation of the three forms of the GadY small RNA. After 10 h of growth, the levels of the GadY(105) and GadY(59) forms of the RNA were substantially reduced while the levels of the GadY(90) RNA remained high (Fig. 2A). We also examined GadY levels in LB medium under low-pH conditions, but we did not detect any changes due to growth in acid (data not shown).
To test whether the increase in expression 6 h after dilution was dependent on the stationary-phase sigma factor σS, we examined GadY RNA levels in an rpoS mutant strain which lacks σS. Northern analysis detected very little GadY from wild-type or rpoS mutant cells grown to exponential phase (2 h). As expected, GadY RNA levels were high in the wild-type cells grown to stationary phase (6 h). This increase in GadY expression was abolished in an rpoS mutant, indicating that GadY expression is dependent on σS (Fig. 2B).
Hfq binding to GadY.A growing number of small RNAs characterized from E. coli affect gene expression through the formation of base pair interactions with mRNAs, thereby exerting regulatory effects on the targets. All of the small RNAs that form base pairs with mRNAs require the activity of the RNA-binding protein Hfq. An earlier study that used microarrays to identify small RNAs that bind to Hfq suggested that GadY may coimmunoprecipitate with Hfq (30). For confirmation of this finding, total RNAs isolated from wild-type and hfq mutant cells, as well as RNAs that immunoprecipitated with control and Hfq-specific antisera, were specifically probed for GadY (Fig. 3). All three forms of GadY coimmunoprecipitated with Hfq, while no GadY was detected in the preimmune control precipitation. We also detected a fourth RNA band in the immunoprecipitation with an Hfq-specific antiserum. We suggest that this was a GadY degradation product that was enriched during the immunoprecipitation procedure, as it was never detected in total cellular RNA extracts. The Hfq protein has been shown to preferentially bind single-stranded AU-rich RNA sequences adjacent to stem-loop structures (29). Based on this observation, we propose that the Hfq binding site is the UUUAU sequence which is postulated to be a single-stranded region adjacent to a stem-loop and is present in all three forms of the RNA (Fig. 1D). The Northern analysis also showed that the strain harboring the hfq mutation contains no detectable GadY transcript (Fig. 3). We suggest that Hfq stabilizes the GadY RNA, similar to what is observed for other small RNAs. In part, the absence of GadY also may be due to the decreased σS expression observed in hfq mutant strains. Together, these results show that GadY is bound by Hfq and suggest that the small RNA acts by base pairing with target mRNAs.
Effects of GadY overexpression.Most of the characterized Hfq binding small RNAs affect the expression of target genes at a posttranscriptional level. Thus, we performed protein gel analysis to look for proteins whose expression was affected by GadY. A strain was constructed that contained the gadY gene cloned behind the strong tac promoter on a multicopy plasmid (pRI-GadY). All three forms of the GadY RNA were expressed at high levels from this plasmid (data not shown). In addition, a control plasmid was constructed with the gadY gene cloned behind the tac promoter in the antisense orientation (pRI-YadG). The protein profile of the GadY overexpression strain was compared with that of wild-type E. coli and the vector and antisense control strains. Protein bands whose intensities were either increased or decreased for the overexpressing strain were excised from the gel, digested with trypsin, and analyzed by MALDI-TOF mass spectroscopy. Based on this analysis, multiple proteins, including DnaK and GroEL, were highly induced, while the TnaA protein was reduced in its expression upon GadY overproduction (Fig. 4A). The peptide masses for one band with increased expression corresponded to the GadA and GadB proteins, which differ by only five amino acids and were indistinguishable in our analysis. The GadA and GadB isozymes are decarboxylase enzymes that convert glutamate to γ-aminobutyric acid, a reaction that consumes an intracellular proton (10). This GadA- and GadB-mediated reaction is one of at least three mechanisms used by E. coli to survive in a low-pH environment (5). Given the close proximity of the gadY and gadA genes on the chromosome, we chose gadA for further study (Fig. 1A).
We next sought to determine if gadA mRNA levels also increased in the presence of GadY, so we performed a Northern analysis to examine gadA mRNA expression in the wild-type strain overexpressing the small RNA. We also examined gadA mRNA levels in a gadX mutant strain overexpressing GadY since GadX is a known transcriptional activator of the gadA gene. When GadY was overexpressed in a wild-type strain, the levels of the 1.4-kb gadA mRNA increased 10-fold above the levels of the wild type alone (Fig. 4B). We also detected two larger RNA species and one smaller RNA species with GadY overproduction. Based on their respective sizes, the larger transcripts may be polycistronic gadAX and gadBC messages, while the smaller transcript is likely a degradation product. The positive regulatory effect of the GadY small RNA on gadA mRNA levels was lost when GadY was overexpressed in a gadX mutant strain, indicating that gadA induction by the GadY small RNA is dependent on gadX.
Possible base pairing between GadY and gadX mRNA.Given that the gadX gene was necessary for gadA induction, we examined the gadX gene to identify a region that could form base pairs with GadY. No complementary sequences could be identified within the open reading frame of gadX. However, a closer examination of gene organization revealed that the 3′ end of the gadY gene and the stop codon of gadX are separated by only 17 nucleotides and that gadX does not display a recognizable Rho-independent terminator. 3′ RACE was performed to identify the 3′ end of the gadX mRNA. This analysis showed that the population of gadX mRNA species has heterogeneous 3′ ends (given at http://dir2.nichd.nih.gov/nichd/cbmb/segr/segrPublications.html ). The majority of the mRNA species identified by RACE contained a 3′ UTR that overlapped the gadY gene by >30 nucleotides. The longest of these mRNA species contained a 3′ UTR that overlapped the gadY gene by 67 nucleotides (Fig. 5A).
GadY positively controls gadX mRNA expression.The finding that the 3′ UTR on the gadX mRNA could potentially form base pairs with the GadY small RNA led us to test whether the expression of gadX mRNA was affected by GadY expression. The GadY-overexpressing plasmid was introduced into wild-type and gadX mutant backgrounds. The cells were grown to stationary phase, total RNAs were harvested, and Northern analysis was performed to detect the gadX mRNA. The GadY-overexpressing strain displayed a dramatic accumulation of gadX mRNA to >20-fold higher levels than those observed in wild-type cells (Fig. 5B). This strong gadX induction would account for the increased expression of gadA upon GadY overexpression. Given that the region of potential GadY-gadX base pairing is at the 3′ ends of both of the transcripts, it is perhaps not surprising that even the overexpression of only the shortest form of GadY, GadY(59), resulted in high levels of gadX mRNA accumulation (data not shown).
We also tested whether gadX mRNA levels are affected in a gadY mutant strain. To accomplish this without disrupting the gadX gene, we constructed a gadY mutant strain in which expression of the small RNA was abolished. Mutagenesis was performed to replace the −10 promoter sequence of gadY (TATATT) with six guanine nucleotides that should no longer be recognized by the RNA polymerase holoenzyme (Fig. 1D). Northern analysis demonstrated that the expression of all three GadY RNAs was severely reduced in this −10 promoter mutant strain (Fig. 5C). This strain was then used to examine gadX mRNA levels in the absence of GadY. Wild-type and gadY −10 mutant cells were harvested during stationary phase, when GadY RNA levels were shown to be maximal. Total RNAs were isolated and tested for gadX mRNA levels by Northern analysis. These experiments revealed that gadX mRNA levels were reduced 4.5-fold in the gadY −10 promoter mutant strain (Fig. 5D). The GadY overexpression and mutant phenotypes indicated that GadY acts in a positive regulatory fashion upon gadX.
3′ UTR of gadX mRNA required for GadY-mediated regulation.The transcriptional regulation of genes involved in the glutamate-dependent acid response is complex, with extensive cross activation and repression occurring between the different regulatory genes. To uncouple gadX transcription from this regulatory circuit, we placed two different forms of the gadX gene under control of the lac promoter in the plasmid pACYC184 (Fig. 6A). One plasmid contained the gadX gene under control of the E. coli lac promoter. This plasmid also contained the gadY gene with the previously described −10 promoter mutation, so GadY was not expressed from this construct (Fig. 6A). A second plasmid was constructed similarly with the gadX gene under control of the lac promoter, but in this instance the entire gadY gene was deleted and replaced with an 85-nucleotide scar (Fig. 6A). This deletion eliminated the region of overlap between the gadY gene and the gadX 3′UTR. These two plasmids were introduced into a strain carrying a chromosomal gadX deletion, and the GadY overexpression plasmid, which carries a compatible replicon, was transformed into each of these strains. The ability of GadY to control the expression of these two forms of gadX mRNA was tested by Northern analysis. In the strain containing gadX with a wild-type 3′ UTR, the gadX mRNA was induced threefold in the presence of GadY expressed from the second plasmid (Fig. 6B). In contrast, the gadX gene with the disrupted 3′ UTR was no longer induced by the GadY small RNA (Fig. 6B). These results indicate that sequences in the 3′ UTR of the gadX mRNA are necessary for GadY-dependent increases in the gadX mRNA levels and suggest that the base pairing of GadY and the 3′ UTR of the gadX mRNA increases its stability.
GadY is unique in several ways with respect to other bacterial small RNAs. GadY is the first example of a bacterial small RNA that can form base pairs with the 3′ end of its target mRNA. Almost all of the characterized Hfq binding small RNAs modulate translation by forming base pairs with sequences spanning and adjacent to the ribosome binding site at the 5′ end of the target mRNA. In general, little is known about the 3′ UTRs of bacterial mRNAs and the type of regulatory signals they contain. More has been learned about regulation at the 3′ UTRs of eukaryotic transcripts, and several microRNAs in eukaryotic organisms have been shown to form base pairs within the 3′ UTRs of their target mRNAs (reviewed in reference 23). We suggest that the formation of base pairs between small RNAs and 3′ UTRs will likely be found for other RNAs. It is worth noting that the gadY-gadX gene arrangement couples the GadY small RNA to its target. Any mutations that occur within the pairing region will create a compensatory change on the opposite strand, with no net loss in complementarity between the small RNA and its mRNA target.
The GadY RNA is also the first E. coli small RNA that positively affects the accumulation of its target mRNA. So far, positive regulation has proved to be rare among RNA regulators. Only two small RNAs, DsrA and RprA of E. coli, have been found to positively regulate gene expression, and both of these function by enhancing translation of the rpoS target mRNA. Currently, no eukaryotic microRNAs are known to positively regulate gene expression, although it is conceivable that microRNAs with functions similar to GadY will be found.
The regulation of the genes involved in the glutamate-dependent acid response is complex and poorly understood. Control of this system has been shown to differ depending on the culture conditions (i.e., complex medium or minimal medium), and it involves multiple regulators (13). Under the conditions we used for our studies, specifically growth in LB broth at pH 7.0, gadX mRNA is expressed during stationary phase in a manner that is dependent on the secondary sigma factor σS (27). We have shown that GadY transcription is also dependent on σS. It was appealing to suggest that the gadX dependence on σS was mediated by GadY. We tested this possibility by overexpressing GadY in an rpoS mutant and found that the gadX mRNA did not accumulate in the rpoS mutant strain that overexpressed GadY (data not shown). This suggests that gadX mRNA accumulation during stationary phase is dependent on σS for transcription initiation as well as on stabilization via the GadY RNA.
We have not ruled out the possibility that GadY regulates multiple targets, some of which are encoded at separate locations of the chromosome. Protein gel analysis indicated that the expression of at least four proteins is directly or indirectly affected by this small RNA. Intriguingly, the TnaA protein, which displays GadY-dependent repression, has been implicated in protecting cells against alkaline stress (4). GadY targets could be regulated by all three forms of the RNA or could require one specific form of the GadY small RNA. Our GadY expression data suggest that the processing or stability of the three GadY small RNAs is different, as indicated by the fact that the 90-nucleotide form of the RNA persisted in late stationary phase while the full-length and 59-nucleotide forms became undetectable. If different forms of the small RNA are required for the regulation of individual target genes, it is possible that the targets are differentially regulated relative to each other.
GadY was shown to efficiently bind Hfq, an RNA binding protein required for the function of all small RNAs that form base pairs with target mRNAs. Although the mechanism of Hfq function is currently not fully understood, in vitro data for the OxyS and Spot42 small RNAs demonstrated that Hfq stimulates base pairing with the target mRNA (21, 22, 29). Current models of Hfq function indicate that the protein may help to unfold regions of base pairing, increase local concentrations of the small RNA and its target, or both. These proposed activities are believed to be essential because base pairing between most trans-encoded (encoded at a different location of the chromosome) small RNAs and their target mRNAs is short and noncontiguous. The GadY RNA is cis-encoded (encoded on the opposite strand from the target), so by definition there is 100% complementarity between the small RNA and its target. The question arises regarding whether Hfq is required for GadY base pairing with the gadX mRNA. It is possible that because of the extensive nature of the base pairing, Hfq is not needed to promote these interactions. This question is difficult to address, however, since GadY RNA levels were severely reduced in the Hfq mutant. Even if Hfq is not required for GadY base pairing with the gadX mRNA, it may play a role in promoting base pairing with an unknown trans-encoded target.
GadY represents a novel mechanism by which a small RNA can positively affect the accumulation of its target, in this case the gadX mRNA. We suggest that base pairing between the GadY small RNA and the gadX mRNA protects the mRNA from degradation by an RNase. This mechanism would be the opposite to the one used by the RyhB small RNA to regulate iron storage proteins. RyhB functions by base pairing with specific mRNAs and rendering the duplex RNA sensitive to RNase E degradation (18). Base pairing between GadY and the gadX mRNA could impede the binding or activity of an RNase that functions at the 3′ end of the gadX mRNA. E. coli encodes two exoribonucleases, polynucleotidephosphorylase and RNase II, whose activities can be blocked by secondary structures that create double-stranded RNA (7, 20). Due to the fact that gadX does not have an identifiable Rho-independent terminator comprised of a stem-loop, the unstructured 3′ end of the gadX mRNA could be particularly sensitive to this type of RNase activity. The base pairing of GadY with the 3′ UTR of gadX mRNA would create a double-stranded RNA duplex that could block the activity of such an RNase. Future experiments will be aimed at elucidating the mechanism of GadY protection of the gadX mRNA as well as determining whether other small RNAs act similarly to stabilize their target mRNAs.
We are grateful to Z. Ma and J. Foster for providing many reagents and sharing information. We thank S. Gottesman and members of our laboratory for helpful discussions, advice on techniques, and comments on the manuscript.
- Received 28 April 2004.
- Accepted 9 June 2004.
- Copyright © 2004 American Society for Microbiology


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Section Branding
Header Content
New Civil War Artifact On Display
Primary Content
Officials at the Georgia Southern Museum in Statesboro say, they now have a rare artifact from the Civil War's largest prison camp.
Its unveiling comes a year after the East Georgia discovery of Camp Lawton.
The camp's unearthing was considered the biggest Civil War find in decades.
Since then, archaeologists have learned more about the site near Millen, including where particular regiments were imprisoned.
Georgia Southern University archaeology graduate student Kevin Chapman says, a letter from the site is even more exciting.
"The letter is truly incredible because it gives us a snapshot of the mindset of a prisoner on November the 11th, 1864," Chapman says. "He writes home and what he's talking about is, 'I'm worried about you,' talking to his wife. 'How are you doing? And if you need money, go visit my friend,' and gives the friend's name."
The letter was in the hands of the soldier's family until it came up for auction.
The university bought it with donated funds for $900.
The new discoveries will go on display in October.
Tags: Georgia Southern University, Millen, Civil War, Statesboro, GPB News, camp lawton, Kevin Chapman


text: TFC Supplies stock a range of building metalwork suitable for a variety of building purposes. This includes, but is not exclusive to, joist hangers, building restraints and straps and brackets and angles and brackets for when extra strength is required. You can shop online or visit our hardware store in Cotes Heath, Staffordshire.


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Mon, 1 June 2015
The boys are joined in the kitchen by journalist Tim Dowling who imparts some much needed wisdom on how to be a good husband, time travel, and not-Mexican food. Sam eats raw hotdogs. James talks about peas for ages. Tim complains about stupid questions. What was the hamburglar doing in a San Francisco parade? What's west of Nebraska? And is Mountain Dew the hillbilly soda of choice?
Direct download: TKIOF-EP33-KAFKAS_MICROWAVE.mp3
Category:Food -- posted at: 1:07am EDT


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Deep-Well Injection
A newish CRS report titles Human-Induced Earthquakes from Deep-Well Injection briefly discusses the Army Chemical Corps decision to dispose of its wastes through deep-well injection. The CRS report describes the process, that lead to
an M 4.8 earthquake that struck northeast Denver, on August 9, 1967 was generally accepted as the largest recorded human-induced earthquake in the United States. The M 4.8 earthquake was part of a series of earthquakes that began within several months of the 1961 start of deep-well injection of hazardous chemicals produced at the Rocky Mountain Arsenal defense plant. The earthquakes continued after injection ceased in February 1966.21 The disposal well was drilled through the flat-lying sedimentary rocks into the underlying older crystalline rocks more than 12,000 feet deep, and injection rates varied from 2 million gallons per month to as much as 5.5 million gallons per month.
For additional background, see the original report on deep-well injection (posted below) and of course, that historical treasure, The Blue Book.
Posted on October 8, 2016, in Deep well injection, Hazardous waste, U.S. Army Chemical Corps. Bookmark the permalink. Comments Off on Deep-Well Injection.


text: Have you ever been to County Mayo, Ireland? Let me start by asking you to do something. Close your eyes and think of Ireland. Let the images and sounds come wafting into your consciousness. Perhaps you are seeing green fields with stone walls? Or a wild blue sea beating off high cliffs and flowing onto […]Continue reading


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It's Monday, which means time for another round of home decor inspiration. This weekend I bumped into an awesome blog called Young House Love. It's by a young couple of "DIY dorks" who share their home decor and renovating adventures and misadventures with the world. It's filled with DIY projects, ideas and inspiration and is a must for anyone working on creating or improving their dream home.
A lot of young couples and small families nowadays are moving away from complexes and townhouses and buying big, older homes that they can renovate, re-do and re-model. My hubby and I also sold our townhouse in May to buy a big old house that needs lots of TLC. It's actually quite a lot of fun and I'm always looking for cool ideas, so the YHB is the perfect place to get inspired.
One of their projects that caught my eye was their Wall of Frames. In my house we too have a long, old-fashioned hallway, that I absolutely love! It's the perfect place to hang family photos. I personally don't like perfectly aligned, matchy-matchy frames next to each other. I prefer to create an effect, with different sized frames, placed in different positions. I did something similar with my wedding photos in my entrance hall, but I'm inspired to take on the whole hallway thanks to the Hallway Full Monty, a step by step project on how to create this effect.
Take a look
The only thing that's left now is to find frames that wont break the bank! I want them to have the same borders, but different sizes. If you guys know of any, comment below and let me know.
All images via Young House Blog


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Victoria Beckham fronts the campaign for her new Estée Lauder makeup collection
Estée Lauder has unveiled the campaign for a second limited edition makeup collection with fashion designer Victoria Beckham. The new Victoria Beckham Estée Lauder Makeup Collection will be available beginning September 2017 at select retailers globally, as well as victoriabeckham.com, esteelauder.com and Victoria Beckham's flagship 36 Dover Street Londonstore.
Victoria Beckham fronts the digital and print campaign, with imagery shot by photographer Lachlan Bailey in New York City.
"Building on the enormous success of our first joint makeup collection last year, we are thrilled to continue our partnership with Victoria for a second, expanded makeup collection launching in September," said Stephane de La Faverie, Global Brand President, Estée Lauder.


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Immigration to deport two Koreans
The Bureau of Immigration (BI) is poised to deport two Koreans wanted by authorities in Seoul for defrauding their compatriots in business dealings, a statement said Sunday.
BI Commissioner Jaime Morente said Lim Chae Beom, 63, and Son Dae Hyon, 45, were arrested last week in separate operations conducted by elements from the bureau's fugitive search unit.
Morente said Beom and Hyon will be deported to stand trial before Korean courts that issued the warrants for their arrest.
"I issued the mission order for their arrest at the request of the Korean Embassy and upon learning that both of them are subjects of red notices from the Interpol," Morente added.
Morente said the Koreans will be expelled for being undesirable aliens as soon as the BI board of commissioners issues the order for summary deportation.
It was learned that BI-FSU operatives arrested Beom on March 10 at his home in Binakayan, Kawit, Cavite while Hyon was apprehended last Thursday in his residence at a subdivision in Cabanatuan City.
BI OIC Associate Commissioner Jose Carlitos Licas said Beom is wanted for defrauding two Koreans of some 238 million won or roughly $210,000 dollars between November 2013 and February 2014.
Beom allegedly enticed the victims to deposit their money to his account by promising he would pay them back after he sold 3,000 kilograms of freshwater eel he intended to import from Okinawa.
Licas said Son is wanted for defrauding a Korean-owned construction firm in Vietnam of more than $141,000 dollars sometime in October 2011.
Son allegedly stole from the company 196 tons of general bars which the victim bought to build a paper factory in Hanoi and which the suspect promised to replace with high-grade bars. DMS