system
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human
Do you need both a -10 and -35 sequence for a promoter to bind?

Just a bit of background. I have a DNA sequence I am trying to interpret and there is a -10 sequence for RpoD (regulatory gene regulator) and there is no -35 sequence that matches a promoter for RpoD. However, at the -17 position I have a sequence that matches AlgT (sigma factor/transcription factor) and a promoter sequence at -42. Is it possible for the RpoD to only bind at the -10 sequence and initiate transcription? Is it possible for AlgT to initiate transcription even if it is 17 nucleotides downstream of the promoter?
gpt
The sequence regions in question are part of the promoter, and help to recruit the transcription multiplex. Both -10 and -35 are not in general required, but a closer match increases the probability of recruitment and promoter clearance. One of the first, most basic regulatory controls is the overall strength of the promoter and how easy it is to transcribe in the first place, i.e. promoter sequences that deviate from the strongest binding sequence are inherently less active.