system
Given the following post, respond as if you're commenting on Reddit:
human
If plasmid has two sites of the same type of restriction enzyme. If we add that type of enzyme, then we will cut the plasmid in both sites, or just cut one ?

If plasmid has two sites of the same type of restriction enzyme. If we add that type of enzyme, then we will cut the plasmid in both sites, or just cut one ?
gpt
Think about it on a small scale, you have a ton of DNA floating around you with a bunch of enzymes mixed in. They will cut anything they have specificity for, and it will happen. Some of the DNA will be cut once, some twice, etc.. all of these fragments would appear in separate bands through electrophoresis. But, assuming a good mixture and perfect reaction, all sites would be cut. Either way, through electrophoresis the size of the fragments can be calculated.

Tl;Dr It's important, for me at least, to visualize the mixture (it's all a crazy busy mess at a small scale)