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Assessment of the Functionality of the Direct PCR-Cloned Putative Promoter Regions of Genes from Arabidopsis Thaliana

ABSTRACT The functionality of the direct PCR-cloned putative promoter regions of predicted genes from Arabidopsis thaliana was assessed. The regions located upstream of the four predicted open reading frames of genes from Arabidopsis thaliana were cloned directly in front of the gus reporter gene in plant transformation vectors. The GUS activity was analyzed in transgenic Nicotiana benthamiana plants carrying chimera promoter:gus genes, as well as after wounding and salicylic acid treatment of the tested plants. Two of the tested promoter regions, derived from putative peroxidase- and cysteine protease-coding Arabidopsis genes, drive strong near constitutive GUS expression in the transgenic lines. The third promoter region, derived from different cysteine protease coding Arabidopsis gene, directs strong salicylic acid inducible transgene expression. The efficiency of utilization of Arabidopsis bioinformation data for direct cloning of functional promoter regions is discussed.