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Nocodazole inhibits polyI:C induction of interferon by mouse macrophages.

Mouse bone marrow-derived macrophages respond to both viral and nonviral inducers of interferon (IFN). The effect of nocodazole, an antitubulin drug, on the induction of IFN by either polyinosinic:polycytidylic acid, or Newcastle disease virus was studied in mouse macrophages. Nocodazole, 0.5 microM and 15 microM, maximally inhibited polyI:C induction of IFN in bone marrow-derived macrophages and resident peritoneal macrophages, respectively, but had no effect on Newcastle disease virus (NDV) induction of IFN.