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Digital in-line holography with numerical reconstruction

Digital in-line holography with numerical reconstruction has been developed in our group into a tool that routinely achieves both lateral and depth resolution at and below the micron level. The experimental and numerical procedures have been incorporated into a program package with a very fast reconstruction algorithm that is now capable of real time reconstruction. This is demonstrated for such diverse objects as fibers, suspensions of microspheres and biological samples (epithelial cells, the eye of Drosophila melanogaster larva), and the advantages are discussed by comparing the holographic reconstructions with images taken with conventional optical microscopy.