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Allyl isothiocyanate as a potential inducer of paraoxonase‐1 ‐ studies in cultured hepatocytes and in mice

In this study, we tested the ability of structure‐related isothiocyanates to induce the antiatherogenic enzyme paraoxonase‐1 (PON1) in cultured hepatocytes. Allyl isothiocyanate (AITC), phenylethyl isothiocyanate (PEITC), and sulforaphane (SFN), but not butyl isothiocyanate (BITC) resulted in dose‐dependent induction of PON1 transactivation in Huh7 cells in vitro. Induction of PON1 due to AITC was inhibited by the selective peroxisome proliferator‐activated receptor γ‐antagonist T0070907. AITC was used in a subsequent in vivo study in mice (n = 10 per group, Western‐type diet) to test its PON1 inducing activity. Unlike in cultured hepatocytes, AITC supplementation (15 mg/kg body weight) did not increase hepatic PON1 mRNA and protein levels in mice. Thus, it is suggested that AITC may be a potent inducer of PON1 in vitro, but not in mouse liver in vivo. © 2011 IUBMB, IUBMB Life,, 2011.