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Endogenous interferences on a DAX system: effects of haemolysis and hyperbilirubinaemia on creatinine and enzyme determinations.

recently, in this journal Guix et al. (1) introduced their results concerning the impairment of creatinine determinations on the Technicon (Bayer) DAX system by haemolysis and encouraged other groups to report on their experience, as well as the setting-up of further investigations dealing with this issue. In our interference study on DAX comprising 19 analytes, turbidity, haemolysis, and hyperbilirubinaemia caused 33 out of a possible 57 analytical interferences (2, 3). The interferoratio was calculated to be 2.1. We focus here on two special aspects of our interference study on the DAX system: the impact of haemolysis on creatinine determinations in order to corroborate the results of Guix and coworkers (1) and the analyte-dependent influence of bilirubin on the measurements of aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), and γ-glutamyltransferase (EC 2.3.2.2) activities.