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Total serum protein N‐glycome profiling on a capillary electrophoresis‐microfluidics platform

We implemented 8‐aminopyrene‐1,3,6‐trisulfonic acid (APTS)‐labeled asparagine‐linked glycan (N‐glycan) profiling on a microfluidic electrophoresis platform. Using 11.5 cm effective length etched channels and 4% linear polyacrylamide as the separation matrix, the major N‐glycans in human serum were profiled in 12 min with a resolution comparable to what is achieved for these analytes on gel‐based DNA sequencers. This demonstration suggests a practical clinical application for high‐speed compact analyzers which might be uniquely based on microfluidic devices.