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Simultaneous HPTLC analysis of Gliclazide and Metformin hydrochloride in bulk and tablet dosage form

This paper presents the development and validation of high performance thin layer chromatography (HPTLC) with a densiotometric detection method for the simultaneous analysis of Gliclazide and Metformin hydrochloride. A separation was performed on silica gel 60F254 plates. The mobile phase comprised of toluene, acetonitrile, ethanol, Ammonium sulphate (0.25%) (4 : 4 : 4 : 3, v/v/v/v). The detection of wavelength was found to be 228nm. The Rf values of GLZ and MET were found to be 0.69 and 0.37 respectively. The accuracy and reliability of the method was assessed by evaluation of linearity 200-1000ng per spot of GLZ and MET. Precision intra-day and inter-day RSD values were always less than accuracy (97.37 for GLZ and 94.8 for MET) specificity in accordance with ICH guidelines. The developed HPTLC method was found to be new, accurate and precise.