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Presidential Task Force on Best Practices for Microbiological Methodology

1. In addition to more precisely defining how preparation and stabilization of inoculated samples should occur, the LOD Working Group has proposed an alternative challenge procedure, Dilution to Extinction (DTE). This methodology has an advantage in that it does not necessarily require the simultaneous analysis of the matrix by a cultural reference method. In certain instances, for example, where the alternative method may be more sensitive than the reference method DTE may have advantages in that the consideration of false positives and false negatives is eliminated. The calculation of the inocula levels of the target analyte organism on the day of initiation of analyses is done only in the organizing laboratory and not in the collaborating laboratories. The performance calculations may be applied to both method comparison and collaborative studies.