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The unequivocal determination of lactic acid using a one‐ dimensional zero‐quantum coherence‐transfer technique

A method based on zero‐quantum coherence transfer for spectral editing of metabolites in aqueous solution in a one‐dimensional experiment is described. Water suppression factors of approximately 8000 were achieved by the use of pulsed B0 field gradients and excellent editing was obtained in a single acquisition. The methyl resonances of both lactate and acetaldehyde were readily observed in a mouse brain homogenate by generat‐ ing zero‐quantum coherence using Gaussian pulses for selective excitation of the respective CH resonances. © 1989 Academic Press, Inc.