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Purification of Catalase Enzyme from Nostoc and its Physiochemical Properties

Catalase(CAT) is a tetrameric enzyme consisting of four identical tetrahedrally arranged that contains a single ferriprotoporphyrin group per subunit. CAT reacts very efficiently with H O to form 2 2 water and molecular oxygen; and with H donors with peroxidase activity. The aim of this research is to purify the enzyme catalase from fresh water algae Nostoc through Sephadox G-75 column, its molecular weight was determined by using polyacrylamide gel electrophoresis and the catalase enzyme stability were observed at various temperature and different pH conditions. Under denaturing conditions, polyacrylamide gel electrophoresis revealed dissociation of a major component of molecular weight 34kDa, which constituted 90% of the total protein of the stained gel, suggesting that the native enzyme is tetrameric. The optimum temperature and pH for the purified enzyme catalase from Nostoc were confirmed at 30°C and pH 7.5.