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Evaluation of Antimicrobial and Antioxidant Activity of Newly Synthesized NMannich Bases from Methyl Salicylate

Isatin reacted with formaldehyde and a number of secondary amines in order to produce the N-Mannich bases of isatin derivatives. FT-IR, 1H-NMR, and mass spectrum data were used to demonstrate that their chemical structures were accurate. An agar diffusion method was used to examine the antibacterial activity of compounds against Gram (+) bacteria (Staphylococcus aureus and Micrococcus luteus) and Gram (-) bacteria (Escherichia coli and Klebsiella pneumonia), with streptomycin employed as control. The antifungal activity of fluconazole against Candida albicans and Aspergillus niger was measured against a benchmark of fluconazole's own performance. Compounds IVc, IVd, and IVe all exhibited potent antibacterial activity, but only compounds IVc and IVd were effective against fungal infections. Compounds IVc and IVe were found to have the highest potential as anti-oxidants among the chemicals that were investigated.