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A manual colormetric assay of triglycerides in serum.

Measurement of serum triglycerides (triacylglycerols) is indispensable for diagnosis of hyperlipemia. Correct diagnosis and treatment depend on triglyceride methodology that is accurate and precise. Many methods involve extraction of the triglycerides, release of glycerin, oxidation of glycerin to yield formaldehyde, and conversion of the formaldehyde to a colored or fluorescent material (1). In the procedure of Nash (2) 3,5-diacetyl-1,4-dihydrolutidine is formed from formaldehyde and acetylacetone; this procedure has been used extensively in continuous-flow procedures (3, 4). Presented here is a manual colorimetric method based primarily on three reports (5-7) in which the Nash sequence of reactions is used. This method is simple, fast, and requires no special equipment. It is precise and accurate and isidealfor use in laboratories that have few requests for triglyceride assays. A continuous-flow procedure has been reported in which the same reactionshave been used (8).