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[A analysis of ELISA on the time-related expression of IL-2 and TNF-alpha during the healing process of wound in rat skin].

OBJECTIVE
In order to explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound.


METHODS
A assay of ELISA were performed on intravital skin wounds(after incision 0.5-168 h) to detect the dynamics expression level of IL-2, TNF-alpha.


RESULTS
The level of IL-2 and TNF-alpha increased at 0.5 h after wounding, then got to a peak at 3 h and 1 h after injure respectively. Rebound of TNF and IL-2 levels were shown at 48 h after wounding, and both cytokine levels were inclined to elevating between 72 h and 168 h after wounding.


CONCLUSION
The cytokine level changes suggest they have a time-related expression during wounds healing process.