{'fraction_non_alphanumeric': 0.0289193302891933, 'fraction_numerical': 0.02511415525114155, 'mean_word_length': 5.032110091743119, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Differential Effect of Paraquat and Hydrogen Peroxide on the Oxidative Stress Response in Vibrio Cholerae Non O1 26/06

ABSTRACT Vibrio cholerae non O1 26/06, a non-pathogenic strain, was subjected to treatment by different concentration of paraquat (PQ) and H2O2. Exposure to PQ for 1 h caused induction of reactive oxygen species (ROS) such as superoxide anion radical (•O2−) and H2O2. At the same time, second stress factor significantly inhibited •O2− production and enhanced the intracellular H2O2 content. The enhanced ROS generation resulted in a significant increase in the levels of oxidatively damaged proteins in comparison to the control variant. Thus, the exposure of V. cholerae cells to PQ and H2O2 promoted oxidative stress. Cell response against this stress includes activation of antioxidant enzyme defence. The treatment with PQ concentrations in the range of 0 - 5 mM resulted mainly in activation of SOD, but not noticeably changed CAT activity in V. cholerae non-O1 26/06. In contrast, effect of H2O2treatment on antioxidant enzyme synthesis in our Vibrio strain was still much more pronounced for CAT than for SOD. Therefore, oxidative stress responses induced by •O2− (generated intracellularly by PQ) and H2O2 demonstrated differential adaptation of Vibrio cells to different toxic agents.