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Adipose-derived Mesenchymal Stem Cells can Alleviate Hypertrophic Scars and Affect the Biological Activity and Oxidative Stress-related Factors of Hypertrophic Scar Fibroblasts


 Background As a fibrotic disease with a high incidence, the pathogenesis of hypertrophic scarring is still not fully understood, and its treatment is also very difficult. In recent years, human adipose-derived mesenchymal stem cells (ADSCs) have been considered an effective treatment for fibrotic diseases, such as keloids. This study mainly explores the therapeutic effect and possible mechanism of ADSCs on hypertrophic scars.Methods After coculture, western blotting and quantitative real-time polymerase chain reaction (qRT–PCR) were used to detect gene and protein expression in hypertrophic scar fibroblasts (HSFs). Flow cytometry was used to detect reactive oxygen species (ROS), and apoptosis, cell proliferation and migration were also detected. A nude mouse animal model was established, the effects of ADSCs on hypertrophic scars were observed, and H&E staining, Masson staining and nuclear factor erythroid 2-related factor 2 (Nrf2) protein content detection were performed.Results Our experimental results show that ADSCs can inhibit HSF proliferation and migration and promote apoptosis. Our experimental results show that ADSCs can inhibit the proliferation and migration of HSFs and promote apoptosis. ADSCs also increase the ROS of HSFs, reduce superoxide dismutase (SOD), NAD(P)H:quinone oxidoreductase 1 (NQO1), and heme oxygenase-1 (HO-1), and reduce the expression of Nrf2 and BCL2. In in vivo experiments, we found that ADSCs can reduce the weight of hypertrophic scars, rearrange collagen fibres, and significantly reduce the Nrf2 content in tissues.Conclusion ADSCs can alleviate hypertrophic scars, promote the rearrangement of collagen fibres, and affect the biological activity of hypertrophic scar fibroblasts and oxidative stress-related factors.