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Improved quantitative method for G6PD deficiency detection

Glucose‐6‐phosphate dehydrogenase (G6PD) deficiency is a common hereditary enzyme defect that affects over 400 million people worldwide. Deficiency in G6PD can cause hemolytic anemia, which can be fatal if it is not treated properly. Mass qualitative G6PD‐deficiency screening methods have been available for some time; however, in this study we developed the first high‐throughput quantitative method for measuring G6PD activity. A patient's blood can be analyzed directly from the original collection tubes on a Hitachi P modular serum work area (SWA), which dramatically reduces the analysis time and the cost of the assay. The results presented here may enable the development of other improved, automated assays of whole‐blood samples. J. Clin. Lab. Anal. 21:107–113, 2007. © 2007 Wiley‐Liss, Inc.