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Addressing Challenges in Ultrastructural TEM Imaging of Chlorophyceae (Green Algae)

The class of Chlorophyceae (green algae) is extremely diverse its families have been isolated from diametric settings spanning from desert crusts to aquatic environments. To classify and analyze their phylogenetic relationship, fine morphological data are critical for elucidation their function and processes such as cell division and desiccation tolerance. Transmission electron microcopy (TEM) was used as a valuable tool for fine ultrastructural imaging and analysis of the main cell features and organelles, as the 'fine structure' provided many vital keys to the understanding of the interrelationships and phylogeny of the algae.