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A Study of Antibacterial Effect of Nigella Sativa Seed Extract on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA)

Objective: A study of antibacterial effect of Nigella sativa seed extract on clinical isolates of Methicillin-resistant Staphylococcus aureus (MRSA). Methods: Samples received at Microbiology laboratory attached to a tertiary care hospital in a period of three months which yield either heavy growth of Staphylococcus aureus or pure growth of S.aureus from patients with wound infection were included in the study. We did punch well diffusion method to determine the antibacterial effect of Nigella sativa seed extract against S.aureus isolates. Results: For MRSA strains, the average zone size for concentrated solution of the seed extract was 25.45± 1.05 mm.; that for 1 in 5 dilution of the extract (166 mg/ml) was 17.75± 1.07 mm and that of 1in 10 dilution (90.9 mg/ml) was 12.30± 0.865 mm. The zone size for 1 in 20 dilutions (47.62 mg/ ml) was not consistent. Out of the 20 MSSA strains tested, 16 (80%) did not show any zone. Out of the 20 MRSA tested, 17 (85%) did not show any zone of inhibition, so the average zone size for 1 in 20 dilution of the extract, was 1.45±2.98 mm for MRSA and 1.10± 2.69 mm for MSSA. For MSSA strains the average zone size for concentrated solution of the seed extract was 25.55± 1.05 mm.; that for 1 in 5 dilution of the extract (166 mg/ ml) was 17.55± 1.05 mm and that of 1in 10 dilution (90.9 mg/ml) was 12.30± 0.923 mm. Conclusion: The present study shows the antibacterial effect of the methanolic extract of N.sativa against our clinical isolates of S.aureus.