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Alternative pathways for DNA-thymine synthesis in stimulated leucocytes.

In vitro transformation of stimulated lymphocytes is frequently evaluated from their ability to incorporate thymidine. This technique measures only one pathway by which cells synthesize DNA-thymine. The purpose of this study was to evaluate the incorporation of various precursors into the DNA-thymine of stimulated leucocytes. It was found that the uptake of thymidine did provide an adequate assessment of total precursor incorporation.