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In Situ Hybridization probing by Transforming Growth Factor β1 marker during prenatal period for rat's primary visual cortex

The objective of this study is to recount the prenatal developmental changes of the primary visual cortex with the space and time appearance of the activity ofthe studied marker through In Situ Hybridization technique. TGF-β1 has a multitude of functions, including both promoting the formation and inhibiting thebreakdown of connective tissue with inhibition of epithelial cell proliferation. Eighteen embryos were used; three embryos for each of the following gestational days (G15, G16, G17, G18, G19 and G20). The reaction was gradual and followed an inside-out gradient from deep to superficial laminae of the rat's primary visual cortex.Cell migration was altered by TGF-β1 in a concentration-dependent manner: at low concentrations, cell migration was promoted whereas at high concentrations, migration was impeded. Therefore, it is an important regulator of the cell behavior throughout prenatal age. 
 