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Process pH control and improvised cell cultivation during copolymer‐induced pelagic cultivation of benthal Chlorella vulgaris

Chlorella vulgaris (C. vulgaris) cultivation particularly at exponential stage is proportional to growth limiting pH reduction, which calls for its stabilization beforehand. This research introduced the use of copolymer polyacrylate polyalcohol (PP) for the first time to harvest dual benefits of its application such as in situ pH control as well as corresponding cell suspension at the surface for better growth kinetics. Free and immobilized cultivation of C. vulgaris was carried out in prepared sugar water in a fabricated photo bioreactor. For free cell cultivation lower (20%) and higher (100%) concentration of BG‐11 broth was used without PP. The immobilized cell cultivation entailed different proportions of PP along with the induction of the macronutrients instead of the broth. It was observed that PP introduction in the medium induced an increase of 32% in the pH value irrespective of the PP dosage applied. C. vulgaris immobilized with optimized PP dosage grew at nearly similar rate as well as incurring compatible chemical oxygen demand (COD) removal compared to that obtained with 100% BG‐11 broth during benthic free cell cultivation. The cell cultivation profile also transpired that every 1 g cell growth during log phase was inducing COD decrease of 13% or fixing 2,100 mg of COD.