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Three-dimensional reconstruction and visualization of confocal microscopic images formed in backscattered light

How can we use visible light to obtain a three-dimensional image of thick, living biological tissues such as the eye or skin? Confocal light microscopy can be used to obtain a stack of optical sections which are stored as digital images. These optical sections can then be processed in a digital computer to form three-dimensional reconstructions. Four-dimensional microscopy is defined as the three-dimensional reconstruction of specimens which are changing with time. At specific time intervals a set of optical sections are collected through the use of a confocal microscope. Each set of optical sections are then transformed into a three-dimensional reconstruction which corresponds to the individual stack of optical sections taken at a given time.