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Electrically promoted protein production by mammalian cells cultured on the electrode surface

Protein production of mammalian cells has been promoted by applying a small constant potential to the surface of an electrode on which cells are cultured. Human carcinoma line of MKN45 cells were cultured on the surface of a platinum‐coated plastic plate electrode. Low d.c. voltage of constant potential was applied to the electrode during 4‐day culture to modulate the production of carcinoembryonic antigen (CEA). The amounts of both secreted and membrane‐bound CEA were dependent on the applied potential during culture. Secreted CEA was more than twice in amount in the potential range from 0.2 V to 0.6 V vs. Ag/Agcl as compared with that of normal culture. In the potential range, CEA was also increased in membrane‐bound form. The potential‐controlled cell culture may have an enhanced effect on protein production.