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Evaluation of reticulocyte counts by flow cytometry in a routine laboratory

Reticulocyte counting using flow cytometry and a membrane‐permeable fluorescent dye, thiazole orange, was evaluated as an alternative to the conventional manual method. The flow cytometric method was more precise (mean c.v. of 4.3%) than the imprecise manual technique (mean c.v. of 22.4%). Linearity was highly acceptable (r = 0.99) over the reticulocyte count range of 1.8–30.1%.