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A collaborative study on RAST inhibition using a common method

A collaborative study was carried out to determine the reproducibility of the inhibition form of the radioallergosorbent test (RAST). Seven laboratories were provided with three coded extracts of cocksfoot (Dactylis glomerata) pollen. All laboratories used a common method of RAST inhibition and were supplied with all reagents required to carry out the assays. In addition, four laboratories used local methods of RAST inhibition. Potency estimates were in reasonable agreement between laboratories when the study method was used but insufficient data were available to assess reproducibility when local methods were used.