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Conversion of In Vitro Cultured Human Monocytes into Effective Presenters of an HER2/neu‐Encoded CTL Peptide Epitope

Tumour‐derived peptides have been surveyed, in a variety of systems, for their ability to elicit cytokine release from class I restricted T cells. Analogous studies on ovarian carcinoma have employed the antigen‐processing defective T2 cell line. Purified dendritic cells (DC) have been reported to act as highly effective APC. A facile method was developed whereby DC‐like cells were generated from monocyte precursors. Herein, evidence is presented suggesting DC‐like cells are superior to T2 with respect to their ability to present a defined CTL epitope associated with ovarian carcinoma.