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A better method for terminal bleeding of mice

A new method for exsanguinating mice is described. The procedure is carried out under CO2-anaesthesia and involves venepuncture of the caudal (inferior) vena cava and gentle aspiration of blood into a syringe. It consistently gives large volumes of blood (1·60-2·50 ml/mouse), free from haemolysis and contamination. The technique is easy, quick and reliable.