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Characterization of Lactobacillus plantarum from wine must by PCR species‐specific and RAPD‐PCR

Aims: Physiological and molecular analysis such as PCR species‐specific and randomly amplified polymorphic PCR (RAPD‐PCR) have been used for typing of Lactobacillus plantarum strains from typical wine must.   Methods and Results: Phenotypic tests such as API 50CH and evaluation of D‐L‐lactate production from glucose were used to perform a preliminary characterization of lactobacilli. Furthermore, 18 strains of lactobacilli were analyzed by PCR species‐specific oligonucleotides based on short sequences of the recA gene.   Conclusions: Four strains were identified as belonging to the L. plantarum species and were further analysed by RAPD‐PCR. The RAPD‐PCR profiles were similar in all strains that had positive results for species‐specific PCR, suggesting that the four L. plantarum strains were closely related.   Significance and Impact of the study: Using PCR species‐specific as a preliminary screening test and then RAPD‐PCR can be as considered the most reliable method of performing a rapid and correct typing of L. plantarum from wine must.