{'fraction_non_alphanumeric': 0.026467203682393557, 'fraction_numerical': 0.012658227848101266, 'mean_word_length': 4.723684210526316, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
A microfabricated fluidic chamber for the perfusion of brain slices

A novel perfusion chamber for brain slice studies was developed, based on the Haas interface chamber principle. The 400-/spl mu/m high chamber is integrated on top of a glass substrate using a standard microfabrication process and is filled with arrays of micropillars to confine and to hold the perfusion fluid within its 11 /spl times/29 mm/sup 2/ area. The micropillars also uphold the brain slice at the "perfusate-gas" interface optimizing the delivery of oxygen as well as of nutrients. With a flow of 30 ml/h, the renewal rate is less than one minute, which permits rapid exchange of the perfusion medium. Successful reproduction of a zero-Mg/sup 2+/ model of epileptiform activity in rat hippocampal slices using this chamber demonstrates its utility for electrophysiological experimentation.