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Immunohistochemical studies on regeneration in cultured epidermal autografts used to treat full‐thickness burn wounds

The normal pattern of healing following culture grafting is not well‐established. The present study describes the immunohistochemcal expression of differentiation markers during various stages of wound healing after resurfacing full‐thickness burn wounds with cultured epidermal autografts. For this purpose, biopsy specimens were obtained from six patients 6 days to 4‐5 years after transplantation. A panel of monoclonal antibodies was used against various differentiation‐specific protein markers, including cytokeratins, involucrin, transgluta‐minase and filaggrin. Findings from early and late biopsies were compared with site‐matched normal skin and cultured epidermal sheets. The persistence of cytokeratins 6 and 16, and to a lesser extent, the abnormal distribution of involucrin up to 4‐5 years after resurfacing burns with cultured autografts was observed. These findings confirm previous studies–that the newly formed epidermis after culture grafting remains in a hyperproliferative state for a long time, which may be caused by the absence of a modulating dermal factor. Our results suggest that keratinocyte maturation following culture autografting does not return to normal for at least 4–5 years after healing of full‐thickness burn wounds.