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Crystal structure of a putative phosphinothricin acetyltransferase (PA4866) from Pseudomonas aeruginosa PAC1

Introduction. In the genome of P. aeruginosa PAO 1 (http://www.pseudomonas.com/) the gene PA4866 encodes a 172 amino acid protein (pita) belonging to the GCN5 family of acetyl transferases (PF00583). Blast searches reveal similar enzymes in a wide range of Gram-negative and Gram-positive organisms. These enzymes are listed either as hypothetical proteins or as putative phosphinothricin acetyl transferases (PAT). PAT is expressed in Streptomyces hygroscopicus and S. viridochromogenes, and catalyzes the acetylation of phosphinothricin, a glutamate analog widely used as a herbicide, which exerts its phytotoxic effect on plants by inhibiting glutamine synthetase. Pita displays 45 and 42% sequence similarity (37 and 35% sequence identity) with the two PAT enzymes, respectively. We now report the crystal structure of this enzyme, as a basis for investigating its function.