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TRPM8 Mediates the Anti‐inflammatory Effects of Eucalyptol in a Mouse Model of LPS‐Induced Pulmonary Inflammation

Eucalyptol (1,8‐cineol), the major ingredient in the essential oil of the eucalyptus leaves and other medicinal plants, has long been known for its anti‐inflammatory properties. Eucalyptol interacts with Transient Receptor Potential (TRP) ion channels among other targets, but it is unclear which of these mediates its anti‐inflammatory effects. In this work, the effects of eucalyptol were compared in WT and TRPM8‐deficient mice in a model of pulmonary inflammation following intranasal administration of lipopolysaccharide (LPS). LPS, a component of Gram‐negative bacterial cell walls, plays a major role in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). 24 hours after LPS intranasal instillation lungs and bronchoalvelolar lavage (BAL) were collected and analyzed for leukocyte infiltration, enzyme activities and cytokine/chemokine levels by qPCR, Luminex and cell counting among other techniques.