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[Effect of exogenous p16 gene on the growth of wild-type p53 human lung adenocarcinoma cells].

OBJECTIVE
To study the effect of p16 gene on human adenocarcinoma cell lines and the posibility of exogenous p16 gene for gene therapy of lung cancer.


METHODS
A p16 gene recombinant expression vector was constructed by cloning p16 cDNA into the pcDNA3 vector at the sites of BamH1 and Xho I, and was transferred by electroporation into p16 gene deficient human lung adenocarcinoma cell lines (A549 and H460) which expressed wild-type p53 gene. The p16 mRNA and protein expression of A549-p16 and H460-p16 cell was detected by Northern blots and immunocytochemistry, respectively. The cell growth properties and cell cycle pattern were aassessed by flow cytometry.


RESULTS
The growth and colony forming efficiency of A549-p16 and H460-p16 cells were significantly inhibited compared with the controls. Flow cytometry showed A549-p16 and H460-p16 cells were arrested in G1 phase of cell cycle, and the percentage of cells in S phase and G2/M phase was decreased significantly. Tumorigenicity of the A549-p16 and H460-p16 cells in nude mice was greatly inhibited.


CONCLUSION
The results show that human p16 gene can suppress the malignant phenotype of wild-type p53 human lung adenocarcinoma cell lines (A549 and H460). p16 gene can play a role in the gene therapy of human lung adenocarcinoma.