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Diagnostics of Malassezia Species: A Review

Abstract Yeasts from the genus Malassezia belongs to normal commensal skin flora of warm-blooded vertebrates. These yeasts may act as opportunistic pathogens and cause skin diseases in humans and animals under certain conditions. The identification of Malassezia species is based on the phenotypic or genotypic diagnostics. The methods used for the phenotypic identification is determined by: the growth on Sabouraud agar, growth on selective media (Leeming-Notman agar, Dixon agar, Chrom Malassezia agar), the ability to utilise different concentrations of Tween, monitoring of the growth on CEL agar (soil enriched with castor oil) and TE agar (Tween-esculine agar), and the catalase test. The genotypic identification uses molecular methods like: the pulsed field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), amplified fragment lenght polymorphism (AFLP), denaturing gradient gel electrophoresis (DGGE), and the DNA sequence analysis.