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Epstein-Barr Virus-specific Leukocyte Migration Inhibition Reaction with Tumor Biopsy Extracts : Correlation with the Presence of Viral DMA 1

The Epstein-Barr virus (EBV)-specific leukocyte migration in hibition (LMI) reaction was used to detect EBV antigens in human tumor biopsies in parallel with nucleic acid hybridization for EBV DNA. None of six EBV DNA-negative tumors gave any significant LMI reaction. Fourteen of 17 EBV DMA-positive tumors gave a significant difference between the migration of leukocytes from EBV-seropositive versus -seronegative donors. One tumor gave a borderline reaction. The two LMI-negatives in this group had only a marginal EBV DNA content. It is suggested that the EBVspecific LMI test may be useful for detecting EBV genomes in tissue and tumor extracts.