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In Vitro Propagation of Florists’ Hydrangea


The effects of repetitive subculturing, cytokinin species and concentration, basal medium, and gelatinizing constituent were studied to maximize shoot multiplication of Hydrangea macrophylla Thunb. ‘Rose Supreme’ shoot tip cultures. Cytokinin treatments of BA or 2iP at 2, 4, 8, 16, or 32 μm were incorporated into woody plant medium (WPM) solidified with 6 g·liter−1 Sigma agar. Cultures grown on 8 μm BA produced the greatest number of useable shoots per 4-week subculture. No treatment stimulated shoot multiplication until the 2nd subculture, after which shoot multiplication remained constant within treatments through the 5th subculture. Results indicate no difference in number of shoots produced per subculture on modified Gamborg's B5, modified Murashige and Skoog basal medium, or WPM when incorporating 8 μm BA and 6 g·liter−1 Sigma agar. No difference in shoot multiplication was observed between 6 g·liter−1 Sigma agar and 2 g·liter−1 Gel-Rite when incorporated into WPM containing 8 μm BA. However, culture fresh weight and shoot length was greater for cultures on Gel-Rite than for cultures on agar. Chemical names used: N-(phenylmethyl)-lH-purin-6-amine (BA) and N-(3-methyl-2-butenyl)-lH-purin-6-amine (2iP).