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Cycle sequencing of filamentous phage DNA using a biotinylated primer and delta Taq DNA polymerase.

We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ∆Taq DNA polymerase in cycle reaction for phage peptide-display library screening. This procedure is specific, rapid, sensitive and safe for the sequencing of large numbers of phage peptide-display colonies. In addition, thermal cycle sequencing with this chemiluminescent image detection protocol provides an inexpensive method for completing a large amount of sequencing reactions. Consequently, this method is especially useful for those extensively working on peptide mimics using phage display libraries.