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Enzyme Immunoassay for Detection of Neisseria gonorrhoeae From Urogenital Samples

A solid-phase enzyme immunoassay (Gonozyme) was used to detect Neisseria gonorrhoeae; the results were compared with the results of cultures taken simultaneously from 201 patients from sexually transmitted disease clinics or family planning clinics. Cultures were positive in 32 of 201 consecutive patients seen in the clinics, while the Gonozyme was positive in 28 of these patients. Overall, five positive cultures were not detected by the Gonozyme, and one positive Gonozyme was not confirmed by culture in the 201 patients tested. The overall sensitivity of the enzyme immunoassay was 84.4% and the specificity 99.4%. The predictive values of a positive and negative Gonozyme result were 96.4 and 97.1%, respectively.