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[The effect of high density lipoprotein and oxidized high density lipoprotein on some secretive functions of endothelial cells].

OBJECTIVE
To investigate the effect of high density lipoprotein and oxidized high density lipoprotein on the synthesis of NO, the activity of NOS, and the secretion of tPA and PAI-1 by cultured human umbilical vein endothelial cells (HUVEC).


METHODS
With the 0. 01 mol/L PBS and N-HDL of 50 mg/L as control, OX-HDL of 5 mg/L,10 mg/L, 50 mg/L were co-incubated with HUVEC for 24 h in vitro. The levels of NO, the activity of NOS, and the levels of tPA and PAI-1 in the medium were detected after the incubation.


RESULTS
Compared with the 0.01 mol/L PBS control, the synthesis of NO(P < 0.05) and the activity of NOS (P < 0.01) were significantly increased in the N-HDL group, but the change of tPA and PAI-1 displayed no statistical significance (both P > 0.05). In the OX-HDL group, the synthesis of NO and the activity of NOS were significantly decreased in a dose-dependent way; the PAI-1 activity(P < 0.05) was increased, but the secretion of tPA (P > 0.05) was not affected.


CONCLUSION
N-HDL significantly increased the synthesis of NO and the activity of NOS secreted by HUVEC, but it had no influence on the generation of tPA and PAI-1. On the contrary, OX-HDL significantly decreased the synthesis of NO and the activity of NOS; it significantly increased PAI-1 activity, but had no effect on the secretion of tPA.