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Comparison of the UV flow dichroism spectra of TMV and several of its mutants

The dichroic ratio spectra of TMV and four of its mutants (YTAMV, GTAMV, HR, and CV4) were determined from 320 to 240 nm. Measurements were also made on particles of ATMV (TMV protein reconstituted with polyadenylic acid) and rods of repolymerized TMV, YTAMV and GTAMV protein, respectively. These cylindrical molecules were oriented in a flow gradient with their degree of orientation being determined from anisotropic light scattering measurements. Taking length distributions into account, the dichroic ratios were then extrapolated to values characteristic of perfect particle alignment.