{'fraction_non_alphanumeric': 0.026881720430107527, 'fraction_numerical': 0.008602150537634409, 'mean_word_length': 5.556338028169014, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Facile radioimmunoassay of estradiol 3-sulfate in plasma during pregnancy.

A facile, sensitive radioimmunoassay of estradiol 3-sulfate in pregnancy plasma was established. Highly specific antisera were raised in male rabbits against a conjugate of 6-oxoestradiol 3-sulfate-(O-carboxymethyl)oxime with bovine serum albumin. After purification of the antiserum, we coupled the crude globulin fraction to CNBr-activated Sepharose-4B and used this for direct radioimmunoassay of estradiol 3-sulfate in plasma without sample pre-treatment such as solvent-extraction or chromatography to remove endogenous interferences. Using this assay system, we determined concentrations of estradiol 3-sulfate in plasma from pregnant women. Concentrations of estradiol 3-sulfate in plasma increased in proportion to those of estradiol throughout gestation, but were about twice as high as those of unconjugated estradiol at each week of pregnancy.