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The effect of thiols on oestradiol binding by components of human malignant breast tumours.

The effect of thiols on the concentration of estrogen-binding sites in supernatant preparations from human breast tumors was studied. Treatment with 1-100 mM of mercaptoethanol increased binding (1.2-fold to more than 6-fold) in 8 of 12 tumors. Pretreatment with dithiothreitrol appeared to have a greater effect than mercaptoethanol. Pretreatment with a thiol-blocking agent (lmM-N-ethylmaleimide) abolished the binding of estradiol. The results indicate that some thiols may be associated with the reduction of disulphide bridges or protection of thiol groups in the high-affinity binding components. It is not yet known whether estrogen-binding is related to human breast-tumor growth.