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Characterization of Hybridization on Diamond Solution-Gate Field-Effect Transistors for Detecting Single Mismatched Oligonucleotides

Using diamond field-effect transistors (FETs) operated in electrolyte solution (solution-gate FETs; SGFETs), a label-free charge detection method between complementary and single mismatched target oligonucleotides is proposed. The probe oligonucleotides immobilized at the activated carboxyl groups of carboxylic aromatic compounds (CACs) on the previously formed amino groups of the diamond surface. The high performance of the diamond surface for oligonucleotides detection was studied with respect to selectivity, sensitivity, and reproducibility of the diamond SGFETs.