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ECOLOGICALLY FRIENDLY BIO-REDUCTION OF GRAPHENE OXIDE BY STAPHYLOCOCCUS WARNERI

This study demonstrates for the first time that the cell-free filtrate of Staphylococcus warneri bacteria can reduce Graphene Oxide (GO). A number of techniques were used to characterize the bio-reduced graphene oxide (BrGO); via the UV-Vis absorbency, scanning electron microscopy (SEM) images, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) analyses. The UV-Vis peak of absorption was around 270 nm. In SEM images, thin, wrinkled nanosheets layered on top of one another were seen. In the FTIR spectra, the distinct peaks relating to oxygen-containing functional groups diminished, whereas the hydroxyl, alkoxy, epoxy, and O-H deformation peaks totally vanished. According to XRD analysis, the peak of diffraction at 2θ= 24° corresponded to a d-spacing of around 0.36 nm. GO sheets have been effectively reduced using the S. warneri bacterium's cell-free filtrate. This method is ecologically friendly, excludes the poisonous reagents usage, cost-effective, besides offering an alternate-safe technique for a suitable graphene production to be applied in biomedical applications.