{'fraction_non_alphanumeric': 0.03747280199854944, 'fraction_numerical': 0.02667418808929003, 'mean_word_length': 4.818096577590248, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 4, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Site-selective nitrenoid insertions utilizing postfunctionalized bifunctional rhodium(ii) catalysts

Site-selective nitrenoid insertions are made possible with a postfunctionalized dirhodium(ii)-catalyst equipped with a remote hydrogen bonding site.


Introduction
Dirhodium(II) complexes act as sensors, 1 show antitumor activity, 2 are capable of cross-linking DNA, 3 and can be used to control peptide structures by binding carboxylate side chains to the Rh core, 4 thus enabling site-specic modications of polypeptides and proteins. 5 They act as Lewis acids to activate alkynes, 6 as well as enynes 7 or serve as hydrogenation catalysts. 8 The well-known Rh(II) carbenoid 9 and nitrenoid 10 transfer catalysts are capable of catalyzing X-H insertions, 11 cyclopropanations, 11a,12 aziridinations, 13 ylide formation, 11a,14 and allylic oxidations. 15 One of the main challenges in Rh(II) mediated carbenoid and nitrenoid insertions is the control of siteselectivity. 16 Davies and coworkers tackled this issue by applying highly site-selective Rh(II) catalysts for carbenoid C-H bond insertions. 17 Bach's group designed a catalyst enabling hydrogen bonding of quinolones, to perform regio-and enantioselective C-H aminations and aziridinations of these substrates. 18 Generally, Rh(II) carboxylates, carboxamidates, and phosphates are prepared in two steps. 19 The rst represents the synthesis of the ligand, which is then subjected to ligand exchange. Early procedures for the preparation of Rh(II) carboxylates made use of Rh(OH) 3 (ref. 20) or Rh(Cl) 3 (ref. 21) and large excess of the ligands. In 1992, a more atom economic protocol for the preparation of these complexes using Rh(II) carbonate, 22 was reported. 23 Ball's group used cis-[Rh 2 (tfa) 2 (-OAc) 2 ] as a precursor for the preparation of metalloenzymes by exchange of the triuoroacetate ligands with peptide carboxylate side chains. 4a The most widely used procedures subject ligands to an exchange with Rh(II) acetate, in which product formation is favored by removal of acetic acid. 24 Functional groups such as unsaturated bonds or nucleophilic moieties are thereby not tolerated (for highly substituted ones see ref. 18a and 25) because of the Lewis acidity of the Rh complex. 26 Focusing on an alternative way to prepare functionalized Rh complexes, we decided to reverse the known approach by designing an appropriate spacer that is introduced rst and allows efficient postfunctionalization, thereby enhancing the functional group diversity (Fig. 1). 27

Results and discussion
The spacer has to bear an acid moiety for attachment to the complex and an additional functional group enabling postfunctionalization. We envisioned that amino acids may be excellent precursors, as the amino group allows efficient functionalization through amide bond formation. However, subjecting unprotected a-amino acids to ligand exchange with Rh 2 (OAc) 4 is not possible as it results in complexes such as 1 involving the binding of the carboxylic acid and the amine to the Rh center (Scheme 1B). 28 Amines irreversibly disrupt the carboxylate bridging structure, also intermolecularly, unless they are sterically crowded or the rhodium core is shielded. 20,25,29 Hence, only a few examples of dirhodium complexes containing an amine have been reported. 4a We decided to investigate the stability of a variety of Rh 2 (A) 4 complexes containing amines. For this purpose, we envisioned to apply Cbz-protected (benzyloxycarbonyl) amino acids to a ligand exchange with Rh 2 (OAc) 4 and to cleave the Cbz group by hydrogenolysis subsequently (Scheme 1A). A variety of Cbz-protected amino acids were synthesized and subjected to ligand exchange using reported conditions. 24a The novel dirhodium(II) carboxylates 2a-i were obtained in yields up to 99%. To our delight Cbz deprotection proceeded quantitatively. As anticipated, it was not possible to isolate complexes 3a-f, because of the presence of the free amino group leading to disruption of the bridged carboxylate structures. The stability of the deprotected complexes was studied with cyclohexyl derivative 3g (Scheme 1B) via time-dependent NMR. While hydrogenolysis of 2g was quantitative, 13 C-NMR analysis of the reaction mixture revealed the disappearance of the Cbz group and appearance of the characteristic bridged carboxylate peak at 191 ppm and an additional carbonyl peak at 173 ppm (Scheme 1B). Thus, the spectrum indicates the presence of the desired complex 3g and another compound. NMR analysis aer 14 days showed the disappearance of both carbonyl peaks and rise of a new peak at 183 ppm, typical for complexes such as 1. We concluded that a sterically crowded ligand should suppress the decomposition of the complex and decided to use g-aminoadamantane carboxylic acid S2. 30 The bulky cage should shield the Rh core and prevent coordination to the metal by the sterically demanding amine. Indeed, deprotection of 2i resulted in formation of complex 3i containing a free amine. The use of gaminobutyric acid further supported the notion that steric bulk is the predominant factor for the design of suitable ligands.
Bench-stable complex 3i was used for postfunctionalization and optimization of the amide bond formation showed that Nsuccinimidyl (OSu) acetate performed best, resulting in quantitative yield of tetra-acetylated S11 (Table S1 †). The succinimidyl esters of 4-pentenoic acid, 4-pentynoic acid, and Boc-L-methionine (tert-butoxycarbonyl) were prepared by EDC-coupling. Postfunctionalization of 3i with the ester of 4-pentenoic acid afforded 77% of 5a (Scheme 2). The alkynyl containing complex 5b was isolated in comparable yield. A control experiment was performed by subjecting 1-alkynyl-3-adamantane carboxylic acid and Rh 2 (OAc) 4 to a thermal ligand exchange. The formation of the desired complex was not observed, but decomposition of the starting material occurred. These new complexes are particular valuable for functionalizations via Huisgen cyclization, 31 thiolene chemistry 32 or olen metathesis. 33  Furthermore, we functionalized 3i with methionine. Carboxylic acid anhydrides can be employed instead of succinimidyl esters, as demonstrated with the synthesis of 5d. 34 Isocyanates were used for the preparation of ureas, affording bifunctional Rh complexes 5e, f (Scheme 2). The structures of the urea complexes 5e and 5f were determined by crystal X-ray analysis. The ligands around the Rh-Rh bond align in local C 2 -symmetry (Scheme 3). 35 One of the main challenges in Rh(II) mediated aziridinations, C-H insertions, and cyclopropanations is the control of siteselectivity. Generally, aziridinations are faster than C-H insertions if sulfamates or sulfonamides are used. 9b,36 However, this trend can change, especially when C]C bonds are sterically crowded or when sulfonimideamides are used as nitrene precursors. 37 Rh(II) catalyzed nitrenoid and carbenoid C-H insertions favor sites that stabilize positive charge. Thus, the reactivity scale for alkanes can be drawn as 3 > benzylic aheteroatom > 2 [ 1. 9b,36a However, catalyst design can alter this trend, e.g., sterically demanding catalysts favor insertions at sterically more accessible C-H bonds. 17b,c,36a As the selective functionalization of, e.g., polyenes, would "greatly streamline the synthesis of complex target molecules", 16b we envisioned to apply bifunctional catalyst 5f in remote site-selective nitrenoid insertion directed by H-bonding. 16a,18a,38 The non-covalent interactions between 5f, containing the key structural moiety 3,5-bis(tri-uoromethyl)phenyl for H-bonding, 39 and an acceptor, should create well-dened spatial relationships. We envisioned farnesol to be a worthwhile target for site-selective aziridination as it has three p-bonds possessing the reactivity trend A > B > C and nine allylic bonds, which may undergo C-H insertion (Table 1). 40 First we installed a hydrogen-bonding acceptor on farnesol by converting the alcohol to carbamate 6. We also performed a conformational analysis in the gas phase using U-GFN2-xTB on nitrenoid complex 5f N with 6. 41 Conformers entailing a reasonable alignment of 6 and 5f N were further optimized in toluene using GBSA as solvent model. 42 Conformers at which the olenic chain of 6 was oriented towards the outside of the cavity of 5f N were not considered as they do not lead to aziridination. The complex depicted in Scheme 4 is the energetically lowest-lying conformer. The computations place the shortest distance between nitrenoid and double bond B at d(N/p B ) ¼ 3.43Å, followed by the least reactive double bond C at d(N/p C ) ¼ 4.24 A, and d(N/p A ) ¼ 6.20Å. Thus, based on steric arguments, catalyst 5f should favor double bond B, although the intrinsic reactivity of 6 should lead to the aziridination of double bond A as the major product. We commenced our study by applying Du Bois conditions 43 with commercially available sulfonamide TcesNH 2 (2,2,2-trichloroethyl sulfamate). The aziridination of 6 with bis[rhodium(a,a,a 0 ,a 0 -tetramethyl-1,3-benzenedipropionic acid)] [Rh 2 (esp) 2 ] afforded a 2.0 : 1.0 (7 A : 7 B ) ratio in favor of double bond A. Likewise, Rh 2 (OAc) 4 afforded a 1.2 : 1.0 ratio ( Table 1). The aziridination of double bond C was not observed.
Bifunctional catalyst 5f favors double bond B with a 1.0 : 1.9 ratio, thereby overcoming the intrinsic reactivity of 6. Note that 5f exhibits the same reactivity as Rh 2 (esp) 2 , which was designed to circumvent the lack of reactivity in intermolecular reactions. 44 With tetrakis [1-adamantanecarboxylate] dirhodium(II) [Rh 2 (O 2 -CAd) 4 ], a sterically similar bulky catalyst as compared to 5f, but not capable of hydrogen bonding, we observed a 1.4 : 1.0 ratio in favor of the intrinsically preferred product 7 A . Higher dilution should suppress the aziridination of substrate not bound to catalyst 5f, thereby enhancing the ratio. Indeed, lower concentrations improved the ratio to up to 1.0 : 5.0 (entry 6). Furthermore, MgO increased the ratio by scavenging the released pivalic acid (entries 10 and 11), which disturbs hydrogen bonding between catalyst 5f and 6. We underscored our hypothesis of hydrogen bonding between 5f and 6 by using 10 equiv. of ethyl-Nethylcarbamate as additive. The additive interacts with the urea moiety of 5f and thus competes for the hydrogen bonding with the substrate. As a consequence, the ratio of 7 A and 7 B decreased from 1.0 : 5.0 to 1.0 : 1.3 (entries 6 and 15). The optimized conditions catalysed by bifunctional complex 5f afforded 40% yield of 7 A and 7 B in a 1.0 : 4.0 ratio (entry 13). The catalysed aziridination utilizing benchmark catalyst Rh 2 (esp) 2 , exhibiting an exceptionally high activity, 44 afforded a similar yield, but in a ratio of 1.1 : 1.0 (7 A : 7 B ). The comparable yields of benchmark catalyst Rh 2 (esp) 2 and 5f conrm the high activity of 5f. Note, catalyst 5f can be used to achieve unique selectivity in the aziridination of polyenes. This proof-of-concept expands the limited number of examples utilizing non-covalent interactions for control of site-selectivity 16a and shows that the novel bifunctional catalysts can be used to overcome intrinsic substrate reactivities by remote hydrogen bonding.
Furthermore, we performed a competition experiment for the nitrenoid C-H insertion of the benzylic position of Bocprotected amine 8 vs. ethylbenzene 9, to demonstrate substrate recognition of 5f via hydrogen bonding (Table 2). Benchmark catalyst Rh 2 (esp) 2 afforded about a 1 : 1 ratio for the two benzylic positions 8 Bn and 10, whereas bifunctional catalyst 5f is capable of discriminating between these two benzylic positions, thereby favoring 8 Bn in a 1.6 : 8.1 ratio; Rh 2 (O 2 CAd) 4 , gave a 1 : 1 ratio. In accord with the reactivity trend, 8 N was observed as the minor product.

Conclusions
We accomplished the isolation of the rst stable Rh 2 (A) 4 complex bearing a free amine. The acylation of this complex enables the incorporation of various functional groups. The bifunctional dirhodium complex was designed and tested in nitrenoid insertions. This catalyst is capable to overwrite the intrinsic reactivity of molecules by remote hydrogen bonding. Future work focuses on the application of other polyfunctional complexes prepared via this new procedure.

Conflicts of interest
There are no conicts to declare.