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Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia.

OBJECTIVE AND METHOD
For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences.


RESULTS AND CONCLUSION
The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.