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Exendin-4 Promotes Beta Cell Proliferation via PI3k/Akt Signalling Pathway

Background/Aims: Prevention of diabetes requires maintenance of a functional beta-cell mass, the postnatal growth of which depends on beta cell proliferation. Past studies have shown evidence of an effect of an incretin analogue, Exendin-4, in promoting beta cell proliferation, whereas the underlying molecular mechanisms are not completely understood. Methods: Here we studied the effects of Exendin-4 on beta cell proliferation in vitro and in vivo through analysing BrdU-incorporated beta cells. We also analysed the effects of Exendin-4 on beta cell mass in vivo, and on beta cell number in vitro. Then, we applied specific inhibitors of different signalling pathways and analysed their effects on Exendin-4-induced beta cell proliferation. Results: Exendin-4 increased beta cell proliferation in vitro and in vivo, resulting in significant increases in beta cell mass and beta cell number, respectively. Inhibition of PI3K/Akt signalling, but not inhibition of either ERK/MAPK pathway, or JNK pathway, significantly abolished the effects of Exendin-4 in promoting beta cell proliferation. Conclusion: Exendin-4 promotes beta cell proliferation via PI3k/Akt signaling pathway.


Introduction
Preservation and restoration of a functional beta-cell mass are effective therapies for diabetes [1]. Thus, understanding of the molecular mechanisms underlying the regulation of beta cell mass in the adult pancreas may substantially boost diabetic research and treatment. During embryonic pancreatic formation, both beta-cell proliferation and beta-cell neogenesis (from pancreatic endocrine progenitor cells that express Neurog3) contribute to the generation of beta cell mass [2][3][4]. However, postnatal beta cell growth has been demonstrated predominantly attributable to beta cell proliferation [5][6][7][8][9][10][11][12][13][14][15].
Incretins are gut-derived circulating peptide hormones that potentiate glucosedependent insulin secretion following meal uptake [16][17][18][19]. Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are two major incretins for controlling the secretion of insulin, glucagon and somatostatin to regulate glucose metabolism through glucose disposal [16]. GLP-1 and GIP are terminated through enzymatic cleavage by dipeptidyl peptidase-4 (DPP-4) followed by renal clearance [16]. So far, it is known that GLP-1 and GIP function predominantly through unique G protein-coupled receptors expressed on beta cells and some non-beta cells [16], whereas the accurate localization of GLP-1 and GIP receptors is not solved due to poor specificity and sensitivity of the available antisera. GLP-1 and GIP have been shown to promote beta cell proliferation and survival in rodents [16]. However, the underlying molecular basis is not completely understood.
Here, we show that Exendin-4 increased beta cell proliferation in vitro and in vivo, resulting in significant increases in beta cell mass and beta cell number, respectively. Inhibition of PI3K/Akt signalling, but not inhibition of either ERK/MAPK pathway, or JNK pathway, significantly abolished the effects of Exendin-4 in promoting beta cell proliferation. Thus, Exendin-4 promotes beta cell proliferation via PI3k/Akt signalling pathway.

Mouse handling
All animal experiments were performed according to the USA National Institute of Health Guide for the Care and Use of Laboratory Animals, and the protocols were approved by the Ethics Committee for Experimental Research, Shanghai Pudong Hospital of Fudan University Pudong Medical Center. Female C57BL/C mice of 12 weeks of age were purchased from the National Resource Center of Model Mice (Nanjing, China). Mice were housed in Pathogen-free environment.

Exendin-4 injection
Exendin-4 (Peninsula Labs, Belmont, CA, USA) was daily intraperitoneally injected to the mice at a dose of 1 nmol/kg body weight) continuously for 7 days. Phosphate-buffered saline (PBS) of same volume was injected to control mice as a sham treatment.

BrdU labeling
For quantification of beta-cell proliferation, 1mg/ml BrdU (bromodeoxyuridine, Sigma-Aldrich, St. Louis, MO, USA) was added into 1% sucrose drinking water, and then given to mice since the first injection

Islet isolation
The mouse pancreas was first perfused with 0.125 mg/ml LiberaseTL (Roche, Nutley, NJ, USA) from the biliary-pancreatic bile duct, then was incubated in 0.125 mg/ml LiberaseTL in a 37 o C shaker at 200rpm for 45 minutes. Histopaque1077 and Histopaque1119 (Sigma-Aldrich, USA) were mixed at a ratio of 9:33 (vol:vol) to generate Histopaque1110. Centrifugation in Histopaque1100 at 1200rpm for 15 minutes without brake (islets are in the solution fraction), following by hand-pickings, was then performed to isolate islets.

Quantification of beta-cell proliferation and apoptosis
Insulin staining was used to identify beta cells. BrdU staining was used to identify S-phase proliferating cells. Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (Tunel) staining was performed with an ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, MA, USA), according to the manufacturer's instruction. The quantification of BrdU+ beta cells or Tunel+ beta cells in vivo was based on 6 sections that were 150µm apart from each other. More than 2000 beta cells were counted in each animal. Ten mice were measured in each experimental condition. The quantification of BrdU+ beta cells in vitro were based on more than 2000 beta cells. Each experimental condition contained t10 repeats.

Quantification of beta cell mass
The quantification of beta cell mass was done as has been described before [8]. Briefly, the pancreata were weighed and then fixed with 4% Paraformaldehyde for 6 hours, and then cyro-protected in 30% sucrose for 24 hours in a way to allow longitudinal sections from tail to head of the pancreas to be obtained. Sections at 150µm intervals from whole pancreas were immunostained for insulin and analysed using NIH Image J software. The relative cross-sectional area of beta cells was determined by quantification of the cross-sectional area occupied by beta cells divided by the cross-sectional area of total tissue. The beta-cell mass per pancreas was estimated as the product of the relative cross-sectional area of beta cells per total tissue and the weight of the pancreas. The beta-cell mass was calculated by examining pancreata from 10 animals for each group.
Cell growth assay A diphenyltetrazolium bromide (MTT) assay was performed to determine cell growth. Fifty islets per well were seeded, treated w/o Exendin-4 and w/o inhibitors, and allowed to grow for 48 hours. Afterwards, the media were removed and the islets were washed with PBS, after which 5g/l of MTT (Amersco, Indianapolis, IN, USA) was added to each well. Four hours later, MTT was removed and 150µl of DMSO was added. The viability of the cells was calculated from the absorption at 570nm with an enzyme-linked immunosorbent assay reader.

Statistics
All values are depicted as mean ± standard error from 10 individuals and are considered significant if p < 0.05. All data were statistically analysed using one-way ANOVA with a Bonferoni correction.

Exendin-4 increased beta cell proliferation in vivo
First, we gave mice Exendin-4 to examine its effects on beta cell proliferation. Exendin-4 was daily intraperitoneally injected at a dose of 1nmol/kg body weight continuously for 7 days. PBS of same volume was injected to control mice as a sham treatment. To quantify beta cell proliferation, BrdU was added into drinking water to the mice since the first injection of Exendin-4 for seven days till sacrifice. We found that the BrdU+ beta cells in sham-treated mice were 1.2±0.2%, while the BrdU+ beta cells in Exendin-4-treated mice were 12.5±1.6%, shown by representative images (Fig. 1), and by quantification ( Fig. 2A). The increases in beta cell proliferation were significant (p<0.05, n=10, one-way ANOVA with a Bonferoni correction). However, Exendin-4 did not significantly affect beta cell apoptosis (Fig. 2B). Moreover, the increases in beta cell proliferation by Exendin-4 resulted in a significant increase in beta cell mass (Fig. 2C, 1.7±0.2 mg in Exendin-4-treated mouse pancreas vs 1.4±0.15 mg in sham-treated mouse pancreas, p<0.05, n=10, one-way ANOVA with a Bonferoni correction). To examine whether beta-cell neogenesis may be involved, we examined the Neurog3 levels in islet-deprived pancreas, and found that Exendin-4 did not significantly affect Neurog3 levels in exocrine pancreas (Fig. 2D). These data suggest that beta-cell neogenesis does not occur after Exendin-4 treatment. Together, these data suggest that Exendin-4 increases beta cell proliferation in vivo.

Exendin-4 increased beta cell proliferation in vitro
In order to study the molecular mechanisms underlying the effects of Exendin-4 in promoting beta cell proliferation in vivo, we examined whether Exendin-4 may similarly increase beta cell proliferation in vitro. Exendin-4 was then given to isolated primary mouse islets, and BrdU was given to label S-phase proliferating beta cells for 48 hours. We found that the BrdU+ beta cells in control islets were 2.5±0.3%, while the BrdU+ beta cells in Exendin-4-treated islets were 28.7±3.1%, shown by representative images (Fig. 3), and by quantification (Fig. 4A). The increases in beta cell proliferation were significant (p<0.05, n=10, one-way ANOVA with a Bonferoni correction). Moreover, the increases in beta cell proliferation by Exendin-4 resulted in a significant increase in beta cell number (Fig. 4B, Exendin-4-treated mouse islets vs sham-treated islets, p<0.05, n=10, one-way ANOVA with a Bonferoni correction) in a MTT assay. Together, these data suggest that Exendin-4 increases beta cell proliferation in vitro.

Exendin-4 increased beta cell proliferation via PI3k/Akt signaling pathway
Different signaling pathways including ERK/MAPK, JNK and PI3k/Akt pathways may be responsible for transduction of signaling to trigger beta cell proliferation by Exendin-4. In order to determine the underlying molecular mechanisms, we gave Exendin-4-treated mouse islets either with 10µmol/l PD98059, a specific inhibitor to ERK1/2, to inhibit ERK/ MAPK signal transduction cascades, or 10µmol/l SP600125, a specific inhibitor to JNK, to inhibit JNK signal transduction cascades, or 20µmol/l LY294002, a specific inhibitor to Akt  We found that only LY294002 significantly inhibited the effects of Exendin-4 in promoting beta cell proliferation, shown by representative images (Fig. 3), and by quantification (Fig.  4A, p<0.05, n=10, one-way ANOVA with a Bonferoni correction). Moreover, the inhibitory effect of LY294002 on Exendin-4-induced increases in beta cell proliferation resulted in a significant reduction in the increases in beta cell number (Fig. 4B, p<0.05, n=10, one-way ANOVA with a Bonferoni correction) in a MTT assay. Together, these data suggest that Exendin-4 increases beta cell proliferation via PI3k/Akt signalling pathway. This model is thus summarized in a schematic (Fig. 5).   Fig. 3. Immunocytochemistry staining for insulin, glucagon and BrdU in Exendin-4-treated islets. Exendin-4 and BrdU were given to cultured mouse islets for 48 hours. Inhibitors PD98059, LY294002 and SP600125 were given to inhibit certain signaling pathways. BrdU, insulin (INS) and glucagon (GLU) triple staining was then performed. Representative images of immunocytochemistry for INS, GLU and BrdU were shown. Control: no treatment. Scale bar is 40μm.

Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry
Here, we show that Exendin-4 increased beta cell proliferation based on BrdU incorporation in vitro and in vivo, resulting in significant increases in beta cell mass and beta cell number, respectively. BrdU specifically labelled S-phase proliferating cells. Thus, a continuous provision of BrdU (7 days in vivo, or 48 hours in vitro) essentially labelled all proliferating cells in the corresponding period, which allows more positive cells to be detected at analysis, as an advantage over short-term BrdU labelling [28][29][30][31].
Although the increases in the beta cell mass or number are modest, it may largely result from the intervention period as short as 7 days in vivo, or 48 hours in vitro. Since   [20][21][22][23][24][25][26][27], it can be expected that longer treatment with Exendin-4 may further increase beta cell mass or number. Another possibility for the relative modest increases in beta cell proliferation and beta cell mass by Exendin-4 may be due to the systemic glucose metabolic control. Since the existing beta cells were not harmed at the time of Exendin-4 treatment, and it is well-known that beta cell mass is tightly regulated for metabolic need, thus the increases in beta cell proliferation and subsequently increases in beta cell mass induced by Exendin-4 may be attenuated by the systemic control, as increased beta cell mass is not necessary in our experimental setting. In line with this notion, in case of beta cell shortage, the effects of Exendin-4 in promoting beta cell proliferation may be substantialized, as has shown previously in diabetes or in beta cell loss [20][21][22][23][24][25][26][27]. ERK/MAPK, JNK and PI3k/Akt pathways may be responsible for signal transduction to trigger beta cell proliferation by Exendin-4. The most frequently applied methods to determine the possible involvement of these pathways were to use loss-of-function approaches to inhibit the target pathways by specific inhibitors. Here, PD98059 is a specific inhibitor to ERK1/2 for suppressing ERK/MAPK, SP600125 is a specific inhibitor to JNK pathway, and LY294002 is a specific inhibitor to Akt for suppressing PI3K/Akt downstream signaling cascades. We found that only LY294002 significantly inhibited the effects of Exendin-4 in promoting beta cell proliferation, and subsequently the increases in beta cell number. It is well-known that Akt activation may induce cell proliferation through phosphorylation modification of either mTor, or FoxO1, which directly regulates cell-cycle activators and inhibitors, e.g. Cyclins, CDKs, p21 and p27 [31][32][33][34]. Since the precise signalling cascades downstream Akt signalling may be cell-type dependent [31][32][33][34], future work may be applied to address these questions.
Since we show that Exendin-4 promotes beta cell proliferation both in vivo and in vitro, it seemed that the effects of Exendin-4 in promoting beta cell proliferation are at least partially conducted directly, and do not require involvement of other cell types. Thus, Exendin-4 should activate PI3k/Akt signalling pathway in beta cells by directly ligand-binding-driven activation of receptor signalling.
Together, our work should provide novel information on the molecular basis of Exendin-4-induced increases in beta cell mass, which sheds new insights into the use of Exendin-4 as a beta cell trophic factor for treating diabetes.

Disclosure Statement
None disclosed.