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Ion channel properties searching by SQUID with real time histogram

Magnetic fields induced from neuromuscular junctions in frogs were measured with a high-resolution dc-SQUID magnetometer in a magnetically shielded room. Scanning the frog preparation automatically, the magnetic field distribution associated with end plate currents was successfully measured. Analyzing the real time histogram of current profile and amplitude distribution conferring the open and close probabilities of Na+ and K+ ion channels, the location of ion channels with their properties was revealed.