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Discriminating between the Effects of Founding Events and Reproductive Mode on the Genetic Structure of Triops Populations (Branchiopoda: Notostraca)

Crustaceans that initially colonize a freshwater temporary pond can strongly bias the subsequent genetic composition of the population, causing nearby populations to be genetically distinct. In addition, these crustaceans have various reproductive modes that can influence genetic differentiation and diversity within and between populations. We report on two species of tadpole shrimp, Triops newberryi and Triops longicaudatus “short”, with different reproductive modes. Reproduction in the tadpole shrimp can occur clonally (parthenogenesis), with self fertilization (hermaphroditism), or through outcrossing of hermaphrodites with males (androdioecy). For all these reproductive modes, population genetic theory predicts decreased genetic diversity and increased population differentiation. Here we use mitochondrial control region (mtCR) sequences and nuclear microsatellite loci to determine if the difference in reproductive mode affects the high genetic structure typical of persistent founder effects. Previous authors indicated that T. newberryi is androdioecious because populations are composed of hermaphrodites and males, and T. longicaudatus “short” is hermaphroditic or parthenogenetic because males are absent. In our data, T. newberryi and T. longicaudatus “short” populations were highly structured genetically over short geographic distances for mtCR sequences and microsatellite loci (T. newberryi: ΦST = 0.644, F ST = 0.252, respectively; T. l. “short”: invariant mtCR sequences, F ST = 0.600). Differences between the two Triops species in a number of diversity measures were generally consistent with expectations from population genetic theory regarding reproductive mode; however, three of four comparisons were not statistically significant. We conclude the high genetic differentiation between populations is likely due to founder effects and results suggest both species are composed of selfing hermaphrodites with some level of outcrossing; the presence of males in T. newberryi does not appreciably reduce inbreeding. We cannot exclude the possibility that males in T. newberryi are non-reproductive individuals and the two species have the same mating system.


Introduction
Aquatic invertebrates that disperse passively via an encysted embryo use a variety of transport methods to colonize new habitats. Abiotic factors, such as water and wind, [1,2,3,4,5] and biotic vectors, such as birds, mammals, insects, amphibians and human activity can disperse invertebrates large distances [3,6,7,8,9,10,11,12,13,14]. Colonization of new habitats by a combination of these factors can be relatively quick, especially if ponds are located in close proximity [1,2]. The potential for dispersal, however, does not always equate to the actual immigration into ponds that is occurring by aquatic invertebrates [15]. It is commonly observed that populations of many aquatic invertebrates can have a high degree of genetic differentiation despite being located in close proximity [16,17,18], a result not expected if contemporary dispersal is frequently occurring between populations.
In cyclically parthenogenetic zooplankton, De Meester et al. [19] emphasized the importance of local adaptation for monopolizing resources, thereby creating genetic differentiation between ponds in close proximity. Boileau et al. [20] concluded that founder events, not contemporary gene flow, have a pronounced effect on the population genetic structure of aquatic invertebrates that produce resting eggs. To demonstrate that genetic ''barriers'' are formed to inhibit immigration into populations, Boileau et al. [20] used simulations to show that F ST does not decay for at least 2000 generations in a large population established by a few founders and subsequently experiencing migrant influx.
In addition to founder events, the mode of reproduction can also influence the amount of genetic structure and diversity in large Branchiopods [21]. A population with individuals that reproduce via selfing experience a heterozygote deficit and decreased diversity due to small effective population sizes [22,23]. In addition, compared to species that outcross, popula-tions of selfing individuals are genetically more isolated because of limited gene flow and often experience demographic fluctuations [22,23].
The tadpole shrimp (Triops sp.) is a passively dispersing aquatic crustacean that has been said to use several forms of reproductive modes including parthenogenesis, hermaphroditism, androdioecy (a mix between outcrossing and hermaphrodites) and gonochorism (males and females that outcross) [24,25,26]. Within Triops populations, low genetic diversity, deviations from Hardy-Weinberg equilibrium, large inbreeding values (F IS ) and large population differentiation have been observed and has been attributed to founder events and the degree of outcrossing between individuals [18,21,27,28,29,30].
Many of the previous studies have focused on Triops populations that are separated by distances of hundreds or thousands of kilometers between sampled ponds [21,27,31]. Large geographic distances between populations makes it difficult to determine if it is the mating system influencing the genetic structure and diversity of Triops populations or if dispersal of encysted embryos is simply limited over long distances. The current study is designed to aid in differentiating between the influence of founding events, dispersal and mating systems by using nine Triops populations located within 30 km and encompassing two putative species with different presumed reproductive modes. Two of the species of Triops in the northern Chihuahuan Desert are T. longicaudatus ''short'' and T. newberryi [27,32]. Different reproductive modes are presumed for T. l. ''short'' and T. newberryi based on the male (absence of a brood pouch) to female (presence of a brood pouch) ratio within populations; T. l. ''short'' is comprised of all females and is assumed to reproduce via parthenogenesis or hermaphroditism whereas T. newberryi is thought to be androdioecious, with populations comprised of hermaphrodites that outcross with males [27]. A recent phylogeny of Triops showed that T. l. ''short'' and T. newberryi are not monophyletic, calling into question whether species status is warranted [33].
The first objective of this study is to assess the genetic structure of each Triops species and determine what factors (founding events or contemporary dispersal) influence population differentiation. Secondly, we compare the effect of different presumed reproductive modes and the degree of inbreeding to the genetic diversity and structure of the Triops populations. We hypothesize, based on population genetic theory, that the androdioecious species will have more alleles, higher allelic richness, fewer private alleles, higher observed heterozygosity, lower F IS and F ST , and relatively greater genetic variance within as opposed to between populations. The last objective is to evaluate whether the two putative species of Triops in southern New Mexico are reproductively isolated in the ponds in which they co-occur.

Sampling Methods and Study Sites
There were no special permits required to sample Triops as they are not listed as a protected species. Triops newberryi and T. l. ''short'' samples were collected in 2008-2011 at nine temporary ponds in southern New Mexico on public land, the Chihuahuan Desert Rangeland Research Center (CDRRC) and the Jornada Experimental Range (JER), both owned by New Mexico State University: two natural playa lakes (PL-07, PL-09), six modified playa lakes (PL-03, PL-05, PL-08, PL-11, PL-33, PL-36), and one man-made flood retention pond (FP-03) (GPS coordinates: Table  S1 in File S1). All ponds were located near Las Cruces, New Mexico, USA within the Chihuahuan Desert (Fig. 1). The two species co-occurred at three of the playa lakes (PL-03, PL-05 and PL-07) (Fig. 1). Live samples were collected using 3 mm mesh seines and immediately placed in 95% ethanol for preservation. A description of field sampling methods, sample locations, and morphological verification of species, are given by Macdonald et al. [32]. Each specimen was evaluated for the presence or absence of a brood pouch to determine sex of the individual. Samples were stored at 220uC until DNA isolation.

Mitochondrial Control Region Sequencing
Extraction of DNA followed a modified version of the HotShot method described by Montero-Pau et al. [34]. Aliquots of 75 ml of the lysis buffer and neutralizing solution were added and samples were incubated at 95uC for 45 minutes. Amplification of the mitochondrial control region (mtCR) was performed by polymerase chain reaction (PCR) with newly developed primers (dloopF 59GCACGAGTTAAGCCGATCTT; dloopR 59CCACAT-GATTTACCCTATCAAGG) for T. newberryi (n = 160) and T. l. ''short'' (n = 66). Reaction volumes of 25 ml consisted of 10 ml GoTAQ Green Master Mix (Promega Corp., Madison, WI), 400 pM of each forward and reverse primer and 1 ng/ml of genomic DNA. PCR reactions were run in a Fisher thermocycler (Fisher Scientific Inc., Pittsburgh, PA) with the following conditions: 94uC for 2 minutes, followed by 35 cycles of 94uC for one minute denaturation, 50uC for one minute annealing, 72uC for one minute elongation and a final extension of 72uC for 15 minutes. PCR products were checked for strength of amplification on a 1% agarose gel. Purification of PCR products was performed with ExoSAP-IT (USB Corporation, Clevland, OH) following manufacturer's protocol. Purified PCR products were then sequenced by NMSU's MOLBIO Molecular Analysis Service in both forward and reverse directions (http://mmas.research.nmsu.edu).
Sequences were aligned using the assembly function in the program Geneious Pro v5.4.6 [36]. Summary statistics, which included number of haplotypes, number of substitutions, number of transitions/transversions, number of polymorphic sites, nucleotide diversity (p), and haplotype diversity (h), for each playa and all samples collectively were obtained by Arlequin v3.5 [37]. Relationships between haplotypes (maternal lineages) were resolved using the program TCS [38] to construct a haplotype network at the 95% confidence level. The appropriate substitution model (TrN+I) for the data was selected based on the Akaike information criterion (AIC) using Modeltest v3.7 [39]. The genetic distance between populations and between haplotypes using the p-distance and the Tamura-Nei model were calculated in the program MEGA v5.05 with uniform rates among sites and gaps treated as missing data [40].
The program Arlequin v3.5 [37] was used to calculate an AMOVA and also generate pairwise W ST values. A sequential Bonferroni analysis [41] was utilized to correct for multiple, nominal tests in the pairwise analysis. Pairwise genetic distances [W ST /(12W ST )] were directly compared to log transformed pairwise geographic distance between the playas to test an isolation by distance hypothesis with the IBDWS v3.21 utility on the web that uses a Mantel test for the analysis [42,43].

Microsatellite Genotyping
Genomic DNA for the microsatellite analysis was extracted by the phenol-chloroform protocol [35] and DNA was stored at 2 20uC. A total of 163 T. newberryi from six ponds and 156 T. l. ''short'' samples from six ponds were genotyped for eight loci developed specifically for Triops species found in southern New Mexico (TL-L-1, TL-S-5, TL-S-9, TL-S-13, TN-6, TN-7, TN-13, TN-14 [30]) and one microsatellite designed for T. cancriformis (TCB-99 [21]). To guarantee the loci are informative for both species [44], loci were chosen based on the primers' ability to PCR-amplify DNA in both species and secondly to contain more than one allele per locus in order to avoid ascertainment bias as described in Ellegren et al. [45]. PCR reactions were done in an UNO II cycler (Biometra, Göttingen) in a 15 ml reaction volume containing 0.3 pmol/ml forward and reverse primers (biomers.net, Ulm), 0.1 pmol/ml Cy5-labled M13 [46], 1X PCR-Buffer (10x reaction buffer without detergent or MgCl 2 ; BD Solis Biodyne, Tartu, Estonia), 2.5 mM MgCl 2 , 0.2 mM DNTPs, 0.04 U/ml Taq-Polymerase (Fire Pol DNA polymerase, Solis Biodyne, Tartu, Estonia) and 2 ng/ml genomic DNA. Microsatellite PCR reaction conditions were 95uC for 3 minutes, followed by 35 cycles of 94uC for 30 seconds denaturation, primer specific annealing temperature for 60 seconds, and 72uC for 60 seconds elongation, before a final extension at 72uC for 3 minutes. Genotyping of all samples was performed on an Automated Laser Flourescence (ALF) II express (Amersham Pharmacia Biotech, Nürnbrecht). Internal and external standards as well as one reference sample (previously sequenced sample) were included on each gel to facilitate consistent scoring across gels. Alleles were scored with the AlleleLinks 1.02 software (Amersham Parmacia Biotech).
The expected and observed heterozygosity of each population and linkage disequilibrium were calculated in Genepop on the Web v4.0.10 [47] and Bonferroni corrections were applied [41]. The dataset was checked for null alleles using the program FreeNA [48] and applying the EM algorithm [49] to compare amplification success of non-specific microsatellite markers on the different species. Values are not reported due to the difficulty of assessing heterozygote deficit vs. null alleles in organisms with high inbreeding [50] and are instead used only as a proxy for successful marker amplification. GenAlEx v6.41 [51] was used for an AMOVA to determine amount of variation between and within populations. The overall and pairwise F ST , their significance values, F IS (the inbreeding coefficient), and allelic richness was calculated in FSTAT v2.9.3.2 as well as a two-sided statistical test to compare allelic richness, observed heterozygosity, F IS and F ST between species [52]. The proportion of selfing (S) in each population was calculated, based on the estimated F IS values, using the equation S = 2F IS /(1+F IS ) [53]. It is noted that there can be a bias when estimating S if the F IS values do not accurately represent inbreeding, and are instead from genotyping error or population substructure [50]. Estimates of inbreeding and selfing rate were compared to the percentage of males in T. newberryi populations, as it would be expected that an increased proportion of males would cause an increase in the rate of outcrossing, therefore lowering selfing estimates. The migration rate (Nm) [54] between populations of Triops was calculated in GenAlEx v6.41 [51]. Similar to the mitochondrial data, genetic distance, defined as F ST /(12F ST ), was compared to log geographic straight line distance between playas to detect the presence of isolation by distance using the IBDWS v3.21 utility on the web that uses a Mantel test for the analysis [42].
To visually determine the genetic structuring between populations within a species, a discriminant analysis of principal components (DAPC [55]) was performed using the adegenet v1.3-5 [56] package in the R platform v2.15.2 [57]. DAPC overlooks the within group variation and summarizes the amount of between group variation, making this method superior for assessing relationships between populations [55]. A factorial correspondence analysis (FCA) of both species together was performed in GENETIX v4.05.2 [58] to visually assess species designation and if hybrid individuals are present in the sample set [59].

Population Structure -mitochondrial Control Region
The T. newberryi mtCR ranged in length from 548 to 551 base pairs long with an overall total of 24 polymorphic sites, 21 substitutions and a transition to transversion ratio of 20:4 (Genbank accession numbers KJ627793-KJ627799). Summary statistics for each playa and for all playas combined are in Table 1.
A TCS statistical parsimony network of T. newberryi mtCR sequences revealed that most individuals are of two haplotypes; 55 individuals had Haplotype 1 and 46 individuals had Haplotype 2 (Fig. 2). The placement of Haplotype 2 and 3 was not certain, but Haplotype 2 and 3 will connect to Haplotype 1 through an additional 12 and 16 mutational steps respectively, at 92% (data not shown). Two singleton haplotypes were observed from PL-07. The average distance between haplotypes was 6.6 mutational steps, excluding Haplotypes 2 and 3. In populations with more than one haplotype, the haplotypes were strongly divergent, with the average number of mutational steps between haplotypes greater than 6.6 steps, except for the singleton haplotypes in PL-07. For example, the 30 samples from PL-11 had two highly divergent haplotypes (with no connection at the 95% confidence level) indicating two genetically different maternal lineages within a population (12 mutational steps between Haplotypes 1 and 2 at 92%).
The overall W ST value was highly significant (W ST = 0.644, P, 0.0033) indicating strong genetic differentiation across all populations of T. newberryi. Estimates of pairwise W ST values indicated significant structure between sampled playas (P,0.0033, sequential Bonferroni correction), except for the comparison between PL-05 and PL-07 (W ST = 0.059, P = 0.009) ( Table 1). AMOVA indicated most of the variation was among populations (64.5%) versus within populations (35.5%) ( Table S2 in File S1). Divergence between populations was not significantly associated with geographic distance (Mantel test, P = 0.65).
Of the 66 T. l. ''short'' samples sequenced, in contrast to T. newberryi, there was only one mtCR haplotype 549 bp in length (Genbank accession number KJ627792). The closest T. newberryi haplotypes to the T. l. ''short'' haplotype were Haplotypes 5 and 6, which differed by 18 pairwise differences. There were 18 polymorphic sites between the T. l. ''short'' haplotype and T. newberryi Haplotypes 5 and 6 consisting of 17 substitutions and 1 indel. No connection was found between the T. l. ''short'' haplotype and the T. newberryi haplotypes in the TCS network at any confidence level.

Population Structure -microsatellites
Results of the AMOVA for T. newberryi indicated that 70.4% of the variation was within populations compared to 29.6% of the variation among populations (Table S2 in File S1). In contrast, the AMOVA for T. l. ''short'' indicated that 35.2% of the variation was within populations compared to 64.8% of the variation among populations (Table S2 in File S1). There was a significant difference (P = 0.001) when comparing the species overall F ST values; T. l. ''short'' has a significantly greater degree of genetic structure and reduced gene flow than T. newberryi. The overall F ST value for T. newberryi was 0.252 and every pairwise F ST comparison was significant after Bonferroni correction ( Table 2). The F ST values ranged from 0.080 between FP-03 and PL-36 to 0.491 between PL-03 and PL-36. Pairwise comparisons between PL-03 and every other sampled playa consistently had the largest F ST values in the dataset (F ST $0.276; Table 2). The overall F ST value for T. l. ''short'' was 0.600 and pairwise F ST comparisons were significant after Bonferroni correction (Table 3) except for the comparison between PL-03 and PL-07. The F ST values ranged from 0.024 between PL-07 and PL-08 to 0.794 between PL-03 and PL-33.
The shape of the DAPC scatterplot for T. newberryi was similar to that representing an island model of population structure, with four population clusters that overlapped (Fig. 3A [55]). The first axis of the DAPC separated the population ellipses consisting of individuals from PL-03 and FP-03 from the other populations (Fig. 3A). There were three distinct clusters in the DAPC scatterplot of T. l. ''short'' populations that were genetically more similar to each other than to the remaining populations [a (PL-09); b (PL-03, PL-07, PL-08); c (PL-05, PL-33); Fig. 3B]. Along the first axis in the DAPC for T. l. ''short'', PL-05 and PL-33 were separated from the other populations (Fig. 3B). The second axis of the DAPC separated PL-09 from the remaining populations. The shape of the T. l. ''short'' scatterplot for the DAPC was similar to the population structure observed in a hierarchical island model [55], different from the island model seen in T. newberryi.
The Nm values between T. newberryi populations were generally below one, indicative of gene flow that is below the threshold for mitigating the effects of genetic drift [60] (Table 2) (Table 3).

Genetic Diversity & Reproductive Mode
In T. newberryi populations, there were in total seven haplotypes, but the number of haplotypes in a population varied from one in PL-36 to five in PL-07. Although FP-03 had the least amount of sequenced individuals (n = 10), it had four haplotypes. The amount of nucleotide and haplotype diversity also varied from the lowest nucleotide and haplotype diversity in PL-36 at zero to the highest nucleotide diversity of 0.014 in FP-03 and the highest haplotype diversity in PL-07 at 0.625 (Table 1). For all populations combined, the nucleotide diversity was 0.015 and the haplotype diversity was 0.747. Modeltest results utilizing the AIC selected the TrN+I model as the best fit for the T. newberryi mtCR data. The smallest Tamura-Nei distance was between PL-05 and PL-07 (0.71%) and the largest occurred between PL-36 and FP-03 (2.34%) ( Table S3 in File S1). The average genetic distance between playas was 1.60% and 1.79% based on p-distance and the Tamura-Nei model, respectively (Table S3 in File S1).
The number of alleles in T. newberryi microsatellite loci ranged from one to 14 with an average of 3.15 alleles across all loci ( Table 1). The microsatellite TN-13, developed specifically for T. newberryi, was the most variable marker (21 alleles), while T. l. ''short'' specific markers were less variable (TLS-13, 2 alleles; TLS-5, 3 alleles; Table 1). The average allelic richness across all loci and populations was 4.12 ( Table 1). The largest allelic richness was in PL-07 (4.36) and the smallest was in PL-11 (2.01). There  was no evidence of linkage disequilibrium. Significant heterozygote deficiency after Bonferroni correction was observed for all loci in each population (Table 1). For T. l. ''short'' the number of alleles ranged from one to seven with an average of 2.37 alleles across all loci (Table 1). Markers developed specifically for T. l. ''short'' (TLS-9 and TLS-5 [30]) were the most and least variable with nine and three total alleles, respectively. The average allelic richness across all loci and populations was 3.34 (Table 1) and was not significantly different to the allelic richness observed in T. newberryi populations (P. 0.05). The largest allelic richness was in PL-07 (2.97) and the smallest was in PL-33 (1.54). Evidence of linkage disequilibrium was found in one playa lake (PL-09) only between loci TCB-99 and TLS-9. Observed heterozygosity was smaller than expected heterozygosity in each population and a significant departure (after Bonferroni correction) from Hardy-Weinberg expectations was observed in all populations ( Table 1). The difference in observed heterozygosity between T. l. ''short'' and T. newberryi was not statistically significant (P.0.05).
In each population of T. l. ''short'' and T. newberryi, the individuals were examined for the presence or absence of a brood pouch, indicative of female or male. Every T. l. ''short'' individual had a brood pouch, congruent with the literature and the proposed parthenogenetic or hermaphroditic reproductive mode [27]. Also consistent with the prediction of T. newberryi as an androdioecious species [27], there were males observed within every T. newberryi population. The amount of males per population ranged from one male out of 30 samples (3.3% males) in PL-07 to eight males out of 28 (28.6%) in FP-03 (Table 1).
Inbreeding analysis indicated a significantly large overall F IS value in T. newberryi: 0.601 (Table 1). All populations had large F IS values, ranging from 0.394 in PL-11 to 0.889 in PL-03 ( Table 1). The estimated proportion of selfing per population was smallest in PL-11 (S = 0.565), largest in PL-03 (S = 0.941) and a (geometric) mean for all T. newberryi populations was S = 0.740 (Table 1). There was no correlation between the values of F IS or S in T. newberryi and the percentage of males per population. For example, PL-03 and PL-11 had the same percentage of males (13.3%), but the F IS and S estimates for these populations were the largest and smallest observed, respectively. Like T. newberryi, inbreeding analysis in T. l. ''short'' indicated a significantly large F IS value over all populations: 0.547 (Table 1). The population F IS values for T. l. ''short'' ranged from 0.277 in PL-08 to 0.658 in PL-05. A statistical comparison of the overall F IS values for the two species of Triops was non-significant (P.0.05). The proportion of overall selfing in T. l. ''short'' populations (geometric mean S = 0.689) was slightly lower than T. newberryi, with the largest proportion of selfing in PL-05 (S = 0.794) and the smallest in PL-08 (S = 0.433) ( Table 1).

Visual Assessment of Species Designation
The FCA separated T. newberryi populations from T. l. ''short'' populations, with the exception of one T. l. ''short'' individual that did not cluster with either species group (Fig. 4). There was one of the eight loci  in this individual (PL07-42; Fig. 4) where the allele combination 157/161 was observed, but was not present in any other T. l. ''short'' at the same locus. The first axis of the FCA accounted for 11.8% of the variation within the data set and the second axis accounted for an additional 7.4% of the variation.  Within T. l. ''short'', there was a clustering pattern in the FCA similar to the DAPC, in which individuals from PL-05 and PL-33 separated along the second axis from the remaining four T. l. ''short'' populations (Fig. 4).

Discussion
The results indicated that populations of Triops were highly structured genetically, even across short geographical distances. There was no evidence of a positive relationship between F ST and geographic distance in either species. The genetic diversity varied across Triops populations, with slightly greater, but non-significant, overall genetic diversity in T. newberryi than in T. l. ''short'' for microsatellites and substantially higher haplotype diversity in the former species. Different models of population structure were observed for T. newberryi (island model) and T. l. ''short'' (hierarchical island) and both species of Triops had low estimated migration (Nm) between playa lake populations. Populations of T. newberryi and T. l. ''short'' were characterized by low diversity, a large degree of inbreeding and high proportions of selfing in all populations. There was no statistically significant difference between any of these measures (F IS , observed heterozygosity, allelic richness) between the species, but all interspecific differences except F IS and the proportion of selfing (S) were consistent with population genetic theory. The presumed androdioecious species (T. newberryi) had higher diversity, fewer private alleles, higher heterozygosity, lower F ST , and greater within-population genetic variance. Lastly, there appears to be a clear genetic distinction between the putative species in southern New Mexico and no evidence of hybridization despite co-occurrence in some playa lakes.

Population Genetic Structure
Despite the potential for dispersal across habitats located in close proximity, playa lake populations of Triops in this study have a high degree of genetic differentiation. For example, PL-03 and PL-05 are separated by a distance of only 1.45 km, however the microsatellite results indicate T. newberryi and T. l. ''short'' are each genetically distinct across the two ponds. There is no evidence of isolation by distance in either species with mtDNA or microsatellites, indicating factors beyond immigration and contemporary dispersal are influential in structuring Triops populations in southern New Mexico.
A survey of T. cancriformis in Europe [18] and of Lepidurus packardi in California [62] attributed the high genetic structure observed between populations to founder events and high selfing rates. Similarly in the current study, there is evidence that founder events and genetic drift, not contemporary gene flow, is responsible for the genetic structure of T. newberryi and T. l. ''short'' in southern New Mexico populations. The migration estimates between most populations are too small (,1) [60] to counteract the effects of genetic drift, suggesting that isolation and drift after the initial founding event is responsible for the genetic structure. There is an  excess of private alleles (3-29%) in each species that can be due to populations that have been separated over time and have experienced little to no gene flow [63]. Despite both species being highly structured genetically, there was a significantly higher degree of differentiation (F ST values) among the T. l. ''short'' populations. The difference is illustrated in the different shapes of the DAPC plots; the hierarchical island model as seen for T. l. ''short'' populations is congruent with increased differentiation as compared to the island model for T. newberryi. The differences in the F ST values between species could be due to a variety of factors including influence from the mating system (discussed below), different population sizes, a genetic bottleneck, and/or distinct colonization events. For example, the T. l. ''short'' PL-05 and PL-33 populations cluster together in the DAPC scatterplot, possibly indicative of the same genetic lineage colonizing and successfully reproducing in both of these locations. In addition, T. l. ''short'' PL-03, PL-07, and PL-08 populations cluster together and have slightly increased Nm values (.1), which may indicate founding lineages and historical connectivity. Zierold et al. [31] demonstrated the possibility of long distance dispersal and range expansions within European populations of T. cancriformis after glacial maximum, but significant differentiation was still observed. This trend observed in populations of Triops is more consistent with founder events being relatively more influential for the genetic structure than contemporary dispersal.

Comparison of Reproductive Mode
The highly inbred mating systems proposed for Triops species in southern New Mexico can also affect the genetic structure of populations. Populations of T. l. ''short'' in this study consist of all females (individuals have a brood pouch) that are thought to reproduce either through parthenogenesis or hermaphroditism [27,32]. Androdioecy is the presumed reproductive mode for T. newberryi because there can be some proportion of ''males'' (individuals lacking a brood pouch) in a population and individuals with a brood pouch can reproduce in isolation [26,27,32]. In the current dataset, there was a ratio of ''males'' in T. newberryi populations ranging from 3.3%-28.6%. In comparing the results of the two Triops species with different presumed mating systems, T. l. ''short'' had fewer alleles, decreased allelic richness, and smaller H E and H O than T. newberryi (Table 1), however the comparison between species for these values were not statistically significant. Despite an absence of statistical significance, a selfing species, such as T. l. ''short'', should have a lower number of alleles, reduced allelic richness and low observed heterozygosity [23]. Also consistent with the effect of mating systems between species is the increase in the greater number of private alleles in T. l. ''short'', the hierarchical island model of population structure and larger between species variation in T. l. ''short'' when compared to T. newberryi.
Inconsistent with the theory for a selfing species is the inbreeding coefficient and proportion of selfing, as T. newberryi has slightly higher values than T. l. ''short'', which should be lower if T. newberryi populations experience some benefit of outcrossing with androdioecious matings between hermaphrodites and males. The reduced microsatellite variation, greater population differentiation and the single mtCR haplotype for T. l. ''short'' that we observed could also be evidence for demographic differences between species including more population bottlenecks in T. l. ''short'' [64], that T. l. ''short'' is an evolutionarily more derived species than T. newberryi [65] or that the persistence of founder events is stronger within T. l. ''short'' populations. The differences in diversity between species could also be due to genotyping error or amplification success of microsatellite markers within a species, however, there is no indication that allele dropout with crossspecies microsatellites is more prevalent in T. newberryi or T. l. ''short'' (results not shown). With mitochondrial genes, Vanschoenwinkel et al. [33] suggested close genetic affinity between T. newberryi and T. l. ''short'' and Macdonald et al. [32] reported only modest differences between Triops species. Despite some males within T. newberryi populations, the F IS values were large for both species of Triops in southern New Mexico indicating an overall high degree of inbreeding. There was also no correlation between the fraction of males in each population and the F IS values of T. newberryi, as would be expected if populations with more males experience more outcrossing, and therefore, a smaller inbreeding coefficient.
Using allozyme data, Sassaman et al. [27] concluded that there was a difference in genetic variation between self fertilizing T. longicaudatus populations and androdioecious populations of T. newberryi in the southwestern United States, but inbreeding values were not given. Zierold et al. [18] did not observe significant differences of F IS values, heterozygosity, or allelic richness between T. cancriformis populations that had some males compared to populations with no males. Velonà et al. [21], however, did observe greater diversity in populations of T. cancriformis that contained males and are presumed to be outcrossing. While the genetic diversity may have differed between androdioecious and selfing populations, the inbreeding coefficient, as in this current study, was not able to distinguish between those populations that have some outcrossing compared to parthenogenetic populations [21].
Based on population genetic theory, there should be strong differences in the diversity and structure of a self fertilizing species compared to a species that experiences some outcrossing [23]. Our results suggest that there are genetic differences between the two species with different presumed mating systems, consistent with the predictions of population genetic theory, however, there was a lack of statistical significance when comparing T. l. ''short'' and T. newberryi. The aforementioned evolutionary history and/or demographic differences between the species may explain why T. l. ''short'' has decreased genetic diversity compared to T. newberryi despite both species experiencing the same level of inbreeding. The inbreeding coefficients and selfing rate estimates are inconsistent with what would be predicted if T. newberryi populations were composed of hermaphrodites that can outcross with males (androdioecy). This might also suggest that although T. newberryi has slightly greater genetic diversity, the role of androdioecy does not alleviate the effects of inbreeding with regard to loss of alleles with genetic drift. In a confirmed androdioecious clam shrimp [66], there was a linear decline in inbreeding as the proportion of males increased, but we did not observe this in our data. Our results call into questions the role of males in T. newberryi. If there is an insufficient level of outcrossing to decrease the estimate of inbreeding, then do males have a reproductive role within a population? We cannot exclude the possibility that individuals lacking a brood pouch are nonreproductive and do not contribute genetic material to subsequent generations. If this is true, then T. newberryi and T. l. ''short'' populations could be composed of hermaphrodites that can outcross with other hermaphrodites and both species could have the same mating system. Outcrossing in both species is supported by the large values of F IS and selfing rates that are not near unity in value, which would indicate complete self fertilization as expected in a pure hermaphroditic scenario. Further clarification of the mating system will require a more direct method, such as a progeny array, to determine the reproductive mode of the Triops species within southern New Mexico. Work is also needed to clarify how adaptable the species are to their respective environments and if these differences contribute to the genetic diversity and structure of T. newberryi and T. l. ''short'' populations.

Putative Species Designation
We presently have analyzed three ponds in which T. l. ''short'' and T. newberryi co-occur in Southern New Mexico in this study. In a global phylogeny of the Notostraca, Vanschoenwinkel et al. [33] concluded that T. longicaudatus ''short'' and T. newberryi were not monophyletic and should be considered conspecifics. It is of interest to know if the species hybridize when they co-occur or if they are reproductively isolated [27,32]. The two species share a majority of their microsatellite alleles, although some alleles are more prevalent in one species.
The FCA of both species showed one individual from PL-07 that was ordinated in an intermediate position between the T. l. ''short'' and T. newberryi population clusters (Fig. 4); both species occur in PL-07. The morphology of this individual was consistent with T. l. ''short'' [32]. The alleles of one microsatellite (TN-14 locus, alleles 157/161) make this sample different. Lacking pedigree information, we are unable to determine if this condition is identical in state or identical by descent and thus a hybrid origin.

Conclusion
The population genetic structure of T. newberryi and T. l. ''short'' in southern New Mexico appears to be strongly influenced by founder events and genetic drift. The high degree of genetic structure between populations at a local scale suggests that contemporary gene flow is not rapidly eroding persistent founder effects. There is evidence that Triops cysts may have the potential for dispersal via a variety of methods [1,2,3,4,5,7,8], but the results here suggest that the potential for dispersal is not realized either because immigration is too low to homogenize the genetic diversity or that migrants have a decreased ability to hatch and/or reproduce in ponds that are non-native. Despite slight differences in the amount of genetic diversity between the presumed selfing (T. l. ''short'') and androdioecious species (T. newberryi), the results indicate similar levels of inbreeding occurring in both species. We conclude both species could be composed of hermaphrodites that can outcross with other hermaphrodites and androdioecy for T. newberryi remains unconfirmed. There are clear differences genetically between T. l. ''short'' and T. newberryi and little evidence to suggest hybridization between the species, which supports T. l. ''short'' and T. newberryi being distinct species as suggested by Macdonald et al. [32]. Additional work could further clarify the mating systems and determine adaptability of migrants to new habitats.