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The RFLP analyses of PdSox8 and PdSox9 in two species of loaches

With the alpha-~(32)P P-dCTP-labeled PdSox8 and PdSox9 fragments of Paramisgurnus dabryanus as probes, the Restricted Fragment Length Polymorphism (RFLP) were analyzed in two species of loaches P. dabryanus and Misgurnus anguillicaudatus. The results showed that the plentiful positive bands were observed in the genomic DNA of 6 individuals (3♀3♂) of P. dabryanus and 6 individuals (3♀3♂) M. anguillicaudatus, which were digested thoroughly by EcoRI and HindIII. The possible reasons were discussed. No sex-specific band was observed.