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[Effects of Mycobacterium tuberculosis Hsp16.3 protein on the autophagy function of mice macrophages].

AIM
To investisate the inhibition of Hsp-16.3 on the autophagosomes formation of macrophages.


METHODS
Mouse RAW264.7 macrophages were induced by rapamycin (50 ng/μL) following infection with M.tuberculosis H37Rv strains, thereafter, co-incubated with Hsp16.3 protein (25 μg/mL). The effects of Hsp16.3 protein on the autophagosomes formation was observed with transmission electron microscope. The expression of autophagy-related genes (atg8) for macrophages was detected by Western blotting.


RESULTS
It was found that rapamycin-induced autophagy of macrophages infected with M.tuberculosis H37Rv enhanced localization of mycobacteria with autophagosomes. Hsp16.3 protein inhibits autophagosome formation and affects M.tuberculosis survival inside infected macrophages. Furthermore, Hsp16.3 protein significantly increased M.tuberculosis colony forming units (CFU), and decreased the expression of microtubule-associated protein light chain-3 (LC3) expression level (P<0.05).


CONCLUSION
The results showed that Hsp16.3 protein inhibits the formation of autophagosomes by regulating the expression of LC3 protein.