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Tc-99m-leukocytes--is it an intracellular labelling?

Radiolabeled leukocytes have a potential for clinical use in detecting sites of inflammation. The increasing interest in the use of Tc-99m-labeled white blood cells (WBC) encourages exploration into the site(s) of binding of Tc-99m to the WBC components. Here we present the differential centrifugation study of the labeled leukocytes using a simple and low cost technique (SnTec). The results show most part of radioactivity bound to cytosol. We concluded that this is an intracellular labeling procedure that has the citoplasm, ribosomes unbound to membrane and soluble molecules as targets.