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Immunofluorescent localization of vitamin B12-binding protein from sow's milk in the ileal mucosa of piglets.

Studies in vitro have shown that the vitamin B12-binding protein from sow's milk enhances vitamin B12 absorption by the neonatal piglet. In the present paper, localization of the milk vitamin B12 binding protein on the brush border membrane of intact ileal enterocytes was demonstrated by an immunocytochemical procedure in which the binding protein was incubated within isolated loops of ileum of anesthetized neonatal piglets.