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Role of stress fibers and focal adhesions as a mediator for mechano-signal transduction in endothelial cells in situ.

Fluid shear stress is the mechanical force generated by the blood flow which is applied over the apical surface of endothelial cells in situ. The findings of a recent study suggest that stress fibers and its associated focal adhesions play roles in mechano-signal transduction mechanism. Stress fibers are present along the apical and the basal portion of the endothelial cells. Endothelial cells respond to fluid shear stress and change their morphological characteristics in both their cell shape and cytoskeletal organization. Atherosclerosis is a common disease of the arteries and it occurs in areas around the branching site of blood vessels where the cells are exposed to low fluid shear stress. The organization of stress fibers and focal adhesions are strongly influenced by shear stress, and therefore the generation of atherosclerotic lesions seem to be associated with the cytoskeletal components of endothelial cells. This review describes the possible role of the cytoskeleton as a mechano-transducer in endothelial cells in situ.


Introduction
Atherosclerosis is a common disease of the arterial blood vessels. It occurs when fatty foods, cholesterol, high blood sugar, and other substances accumulate in the walls of arteries form "plaques". The area around the branching point of blood vessels is the specifi c location of atherosclerotic lesions. Many studies have addressed the development of atherosclerosis. Specifi cally, during the early atherosclerotic development stage, plaques tend to be organized at the branching point of the vessels where the blood fl ow speed is low or a disturbed fl ow is identifi ed. Therefore, the hemodynamic force generated by the blood fl ow is thought to play an important role in the organization of atherosclerosis. A previous study demonstrated that the disturbed fl ow at the bifurcation point of arteries is a determinant for the development of arteriosclerosis, and the steady laminar fl ow of high fl uid shear stress is atheroprotective (Berk 2008). However, it is still unclear how this disease develops in the regions of low blood fl ow low at the branching site of the blood vessels.
Endothelial cells are capable of responding to fl uid shear stress by altering their morphology, the distribution of cytoskeletal components, expression of certain types of proteins, etc. In endothelial cells, actomyosin-based cytoskeletal components, called stress fi bers, are observed both in vivo and in situ. In vivo experiments demonstrated that stress fi bers in endothelial cells respond to fl uid shear stress and increase in number and thickness. In an artifi cial coarctation zone, where shear stress is expected to be comparatively high, stress fi bers in both the apical and basal portions dramatically increased in number and thickness . Focal adhesion-like structures which are detected at the end of stress fi bers are more enlarged in the coarctation area, especially in the apical surface of endothelial cells . In addition, stress fi bers and their intimate association sites with the plasma membrane are closely attached in both the apical and the basal portions of endothelial cells, and they seem to a play role in the transmission of fl uid shear stress forces (Katoh et al 1995;Kano et al 1996). We showed that there are certain areas where the apical plasma membrane and apically located stress fi bers are tightly connected at the lumina surface of endothelial cells. We call such areas "apical plaques". They seem to play an important role for sensing signal transduction of fl uid shear stress, because apical plaques located at the luminar surface of endothelial cells where the fl uid shear stress directly applied (Kano et al 1996. Cytoskeletal organization and its distribution changes drastically according to the magnitude and the direction of fl uid shear stress applied over the endothelial cells. A fl ow sensing structure might be located at the part of the lumina surface of the cell where the fl uid shear stress directly applied. On the surface of endothelial cells, cytoskeletal components that directly connect between the apical plasma membrane and internal side of the cell, called "apical plaques" are observed. The apical plaque is site where the fl uid shear stress is directly applied (Katoh et al 1995;Kano et al 1996Kano et al , 2000. Bundles of actin filaments are associated with the plasma membrane at sites where the cells form connections with the extracellular matrix or cell-cell association sites. Stress fi bers are composed of actin fi laments formed by the contractile interaction of actin, myosin, alpha-actinin, tropomyosin, to name a few forming a sarcomere-like structure (Byers et al 1984;Langanger et al 1986;Burridge et al 1988). Well-spread cells exert tension on the adhering extracellular matrix and their stress fi bers were preextended (Dembo and Wang 1999;Lu et al 2008). Stress fi bers directly terminate at focal adhesions where several structural proteins that connect the cell membrane to the underlying substrates are accumulated. Isolated stress fi bers from cultured fi broblasts contracted in response to Mg 2+ -ATP with Ca 2+ (Katoh et al 1998). The contraction of nonmuscle calls, including smooth muscle and stress fi bers, is caused by the phosphorylation of a myosin light chain (MLC) by a calmodulin/myosin light chain kinase (MLCK) system in a calcium dependent manner. On the other hand, Rho (Ras homology) proteins are small GTPases that are involved in signal transduction in the cell. Rhoassociated kinase (called Rho-kinase, ROCK2, Rokα) (Isenberg et al 1976;Leung et al 1996;Matsui et al 1996;Katoh et al 2007b) is effectors of Rho small GTPase. The activation of Rho-kinase, which is downstream of Rho, is known to modulate the organization of stress fi bers and focal adhesions (Ridley and Hall 1992;Amano et al 1996aAmano et al , 1996bAmano et al , 1997Katoh et al 2007b). Moreover, Rho-kinase either directly or indirectly phosphorylates the MLC in a Ca 2+ -independent manner in vitro system (Amano et al 1996a(Amano et al , 1996b. Our recent experiments revealed that the actomyosin contraction system could be regulated by at least two independent systems: namely, the one Ca 2+ -dependent calmodulin/MLCK system, while the other is the Ca 2+ -independent Rho-kinase system. Cell tension organized by stress fi bers is essential for many kinds of cell behaviors, such as cell movement, anchoring cells to extracellular matrix, the development of embryos, mechano-signal transduction into the cell, and so on. Rho and its associated Rho-kinase seems to play important roles for the maintenance of endothelial cells in situ , 2008van Nieuw Amerongen and van Hinsbergh 2007;van der Heijden et al 2008).
Although many of stress fi bers run along the basal portion of the spreading cells, some of these run from the basal to the apical portion of the cell (Katoh et al 1995). Such stress fi bers have been observed in both cultured and in in situ cells. On the other hand, focal adhesions are closely associated with both the apical and basal portions of endothelial cells, therefore these stress fi bers seem to play a role in the transducer mechanism for the outside-in signaling of endothelial cells Katoh et al 2007a;Hayakawa et al 2008). This review will discuss the contractile activity generated by stress fi bers and its organization in the cell. This review will also discuss the possible role of stress fi bers, together with their associated focal adhesions, as a mediator for mechanosignal transduction.

Stress fi bers as a model for the actomyosin contractile system
The contractile mechanism of stress fi bers has been previously reported by several researchers (Hoffmann-Berling 1954;Isenberg et al 1976;Kreis and Birchmeier 1980). A procedure has been established to isolate stress fi bers from a cultured cell system without any loss of contractility (Katoh et al 1998;Kano et al 2000). Using an extraction solution containing Triton X-100 detergent, stress fi bers can be isolated from cultured fi broblasts, which can be used as a model system to represent the nonmuscle actomyosin contractile system. This model was used to study the regulation of stress fi ber contraction in vitro. Stress fi bers Role of stress fi bers and focal adhesions for mechano-signalling have a contractile structure (Katoh et al 1998. The contraction of isolated stress fi ber is dependent on Ca 2+ and Mg 2+ /ATP. The phosphorylation of the MLC occurs when stress fi bers are treated with Ca 2+ and Mg-ATP. This MLC phosphorylation is inhibited by protein kinase inhibitors, ML-7, one of which is specifi c for MLCK. The contraction of isolated stress fi bers is inhibited by ML-7, thus indicating that MLC is phosphorylated by MLCK in a Ca 2+ -dependent manner. The contractility of the extracted cells and actomyosin containing structures of nonmuscle cells has been previously reported. The extent of contraction of smooth muscle cell is 38% of the original length (Fay et al 1982) and the speed of contraction is 10 μm/s (Small 1977). The nonmuscle contractile apparatus of the circumferential microfi lament bundles in retinal pigmented epithelial cells represent 40% of the original length (Owaribe and Masuda 1982), the contractile ring of cells represent about 30% (Cande 1980;Mabuchi et al 1988), the kidney proximal tubules of the rat represent 20% (Murakami and Ishikawa 1991), or the brush border of the intestinal epithelium represent slight contraction (Rodewald et al 1976). Several investigators have indicated that stress fi bers are truly contractile and they can indeed regulate the isomeric tension within the cell (Burridge 1981). A stress fi ber model with Mg 2+ /ATP contracts to 60% of the original length in a micro-dissected model (Isenberg et al 1976), 80% in glycerol extracted cells (Kreis and Birchmeier 1980) and 75%∼80% in digitoninextracted cell model (Kreis and Birchmeier 1980). The essential activity for the contraction of stress fi bers must be caused by the phosphorylation of MLC. Phosphorylation of the MLC occurs when isolated stress fi bers are treated with Ca 2+ and Mg-ATP following isolation with the Triton X-100-based extraction solution. Under those conditions, phosphorylation of MLC is inhibited by protein kinase inhibitor, which is a specifi c inhibitor for MLCK (ML-7). The contraction of isolated stress fi bers is completely inhibited by ML-7, thus indicating that MLC is phosphorylated by MLCK in a Ca 2+ -dependent manner.
Many stress fi ber models isolated from fi broblastic cells, that are simply extracted, represent a limited extent of the contraction activity, showing only 70%∼80% of the original length. In our model, the completely isolated stress fi bers contract to 23% of the original length (Katoh et al 1998). The speed of tail recoiling of living fi broblasts is 1.5 μm/s (average: maximum 3.5 μm/s) (Chen 1981). The extent of contraction of the stress fi ber is more substantial than that observed for other types of cell contractility. Stress fi bers thus demonstrate the fastest actomyosin based contractile system found in nonmuscle cells.
Rho A is a Rho-protein expressed in most types of the cell. Both the reorganization and contraction of stress fi bers are observed when cultured fi broblasts are treated with LPA or bombesin Hall 1992, 1994). Because these drugs are Rho A activators, this response appears to be mediated by Rho A Hall 1992, 1994). Recent studies have also demonstrated the activation of Rho-kinase, which is a downstream regulator of Rho A, is observed in stress fi bers (Katoh et al 2001a(Katoh et al , 2001b. Moreover, both biochemical and immunofl uorescence studies revealed Rho-kinase to have binding activity with myosin II and is thus located on the stress fi bers (Kawabata et al 2004;Nakayama et al 2005). The above reports indicate that the Rho-kinase and myosin II work together on the stress fi bers to control the contractile activity of the stress fi ber. Biochemical studies also have shown that Rho-kinase phosphorylates the MLC of isolated smooth muscle fi bers (Amano et al 1996b;Chihara et al 1997) and detergent extracted fi broblasts Amano et al 1998). In vitro experiments have revealed that Rho-kinase directly phosphorylates MLC (Amano et al 1996b). Rhokinase also known to dephosphorylate MLC indirectly with the inhibition of the myosin phosphatase activity (Amano et al 1996a). The de-phosphorylation of myosin phosphatase with active Rho-kinase causes the phosphorylation of MLC, thus resulting in the contraction of stress fi bers (Katoh et al 2001a(Katoh et al , 2001b. Stress fi bers can be isolated by a glycerol-based extraction solution without Triton X-100. Stress fi bers isolated with glycerol-based extraction buffer without detergent contract when Mg 2+ -ATP without Ca 2+ is added. Since the calmodulin/MLCK contractile system essentially needs Ca 2+ ion, this Ca 2+ -independent contraction is caused by Rho-kinase activity (Katoh et al , 2001b. The contraction of stress fi bers isolated without detergent was not inhibited by MLCK inhibitors (Katoh et al 2001b). A biochemical and immunofl uorescence analysis revealed that glycerol-isolated stress fi bers contain both Rho A and Rho-kinase in the stress fi ber. However, Triton X-100 isolated stress fi ber did not contain Rho A and Rhokinase, on the immunoblotting and immunofl uorescence level. The above observations indicate that contraction of Ca 2+ -independent stress fi bers contraction is simply caused by the Rho-kinase activity. Such contraction of stress fi bers isolated without detergent is inhibited by the activity of Rhokinase specifi c inhibitors, Y-27632. These results indicate the stress fi ber contraction to be regulated independently in two ways: namely, a Ca 2+ -dependent calmodulin/MLCK system and a Ca 2+ -independent Rho-kinase system (Katoh et al 2001a).

Mechanism of stress fi ber contraction in Ca 2+ -dependent and/or Ca 2+ -independent manner
As we mentioned above, stress fi ber contraction is dually regulated: namely, by the Ca 2+ -dependent calmodulin/MLCK system and the Ca 2+ -dependent Rho-kinase system. Kinetic analyses of isolated stress fi ber contraction suggest that the Ca 2+ -independent system causes a slower contraction (Katoh et al 2001a(Katoh et al , 2001b. On the other hand, the Ca 2+ -dependent MLCK system causes a faster contraction than the Rhokinase system. In the Rho-kinase system, both the speed and extent of contraction are less than that induced by the typical MLCK system. Two independent regulation systems exist in stress fi bers together and seem to regulate different types of contraction. The calmodulin/MLCK contractile system supports transient and fast contraction. Hence, the Rho-kinase contractile system supports continuous and slow contraction. To understand the roles of MLCK and Rho-kinase for the contraction and the organization of stress fi bers in living human foreskin fi broblasts, we expressed green fl uorescent protein labeling actin, and treated with several MLCK or Rho-kinase inhibitors (Katoh et al 2007b). In result, the Rho-kinase inhibitors, HA1077 or Y-27632, caused a disassembly of the stress fi bers that were located at the central portion of the cell within 1 hr. However, the stress fi bers located in the cell periphery were not so severely affected by the Rho-kinase inhibitors. Fluorescent images revealed many thin stress fi ber bundles, together with the focal adhesion at the both end of the stress fi ber, at the center of the cell. These stress fi bers are known as "central stress fi bers" (Katoh et al 2001a). In addition, thicker stress fi bers associated with focal adhesions are localized at the arched part of the lateral side of the cell. These stress fi bers are known as the "peripheral stress fi bers". In both the phase contrast images and fl uorescent images, the two types of stress fi bers were easily distinguishable. Moreover, electron microscopy also revealed that these two types of fi bers are typical stress fi bers and are associated with focal adhesions, although the location and thickness are different.
The treatment of living cells with Rho-kinase inhibitor, Y-27632, specifi cally causes a disassembly of the stress fi bers located at the central portion of the cell. The peripheral stress fi bers are slightly reduced in thickness, however, they are hardly affected by the Rho-kinase inhibitor. The arched region at the leading edge of the cell gradually protrudes after the Rho-kinase inhibitor treatment, thus forming arches that are less accurate. The local movement of the plasma membrane at the cell periphery can be explained by the relaxation and loss of contractile tension at the cell. The relaxation of the cytoplasm and the cell cortex might be caused by the inhibition of Rho-kinase activity. When the activity of Rhokinase is high, the cell tension generated by the contraction of stress fi bers is respectively high. The activity of Rho-kinase seems to play a role mainly in controlling the cell tension generated by the contractile activity of stress fi bers, which result in the transmission of fl uid shear stress force applied over the surface of the cell to the inside of the cell.

Localization of stress fi bers and focal adhesion in cultured and in situ cells
In cultured fi broblasts, analyses by confocal laser scanning microscopy have revealed that both stress fi bers and focal adhesions are present along both the apical and the basal portion of the cell. Such stress fi bers are found in cultured cell systems, including fi broblasts and endothelial cells and in in situ endothelial cells (Katoh et al 1995;Kano et al 1996Kano et al , 2000. In addition to those present at the basal side of the cell, many stress fi bers run from the basal side to the apical side of the cell. Such stress fi bers are known as "apical stress fi bers" and the macromolecular composition of these stress fi bers is the same as that of general stress fi bers (Katoh et al 1995;Kano et al 2000). These stress fi bers attach themselves to the apical plasma membrane of the endothelial cell with plaque like structures, which contains major focal adhesion associated protein, vinculin. Detailed observations have revealed that the attachment site of the stress fi bers at the apical plasma membrane thus forms focal adhesion like structures (Figure 1). When such attachment sites are stained with antibody against general focal adhesion associated proteins, such as vinculin, talin, integrin or fi bronectin, they tend to show spotty plaque structures at the apical portion of the cell. These plaques are known as "apical plaques". Several transmembrane proteins, such as integrin beta-1, fi bronectin receptor and tyrosine-phosphorylated proteins are observed in apical plaque and such observations suggest that these apical plaque seems to act as a mechano-transducing structures at the apical surface of the cells. These apical stress fi bers are not required by the cell to substrate adhesion sites, but rather they are needed to anchor the stress fi bers to the apical plasma membrane in the cultured fi broblasts, because they are located at the dorsal side of the monolayer Role of stress fi bers and focal adhesions for mechano-signalling of endothelial cells. These apical stress fi bers are attached to the plasma membrane in the apical portion of the cell principally in the same way as in basal focal adhesions. Basal focal adhesion can transmit outside-in signals from the extracellular matrix to the inside of the cell, so that the apical plaque thus seems to play a crucial role in the response to extracellular signaling, such as fl uid shear stress applied over the endothelial cells.
Endothelial cells lining the inner surface of blood vessels are directly exposed to blood fl ow mechano-signals. Various types of endothelial cells are known to respond to the blood fl ow, for example, by changing their cell shape, cell migration and the re-organization of the stress fi ber and focal adhesions.
Many previous reports demonstrated that the mechanical forces generated by the blood fl ow have been shown to induce the redistribution of stress fi bers and focal adhesions (Franke et al 1984;Ookawa et al 1992;Davies et al 1994;Girard and Nerem 1995). In both in situ and in vitro endothelial cells, under high fl uid shear stress applied to cells, they organize thick stress fi bers along the direction of the blood fl ow. In in situ endothelial cells, the shear stress generated by the blood fl ow is the frictional force that acts directly on the apical surface of the endothelial cells. Therefore, shear stress-related signal transduction is highly expected to initially start at sites where "chemical signaling events" caused by the mechanical signaling may be initiated. Therefore, one possible candidate to receive mechano-signaling by the blood fl ow is the "apical plaque". Observations by confocal laser scanning microscopy revealed that thick stress fi bers are present mainly along the basal portion of the cell. However, thin stress fi bers are also found in the apical portion of the cell (Kano et al 1996). Such apical stress fi bers observed in the endothelial cells run along the whole length of the cell in the same direction as the blood fl ow (Kano et al 1996 (Figure 2). Some stress fi bers run vertically, connecting the basal and the apical side of the plasma membrane as in cultured fi broblasts (Katoh et al 1995). Focal adhesion associated proteins, such as vinculin, talin, paxillin or fi bronectin receptors have been also located in both the basal and the apical portions of the cell-forming plaque (Kano et al 1996.

Possible mechanisms of stress fi bers as a mediator for mechano-signaling
Fluid shear stress is a determinant of endothelial cell morphology. It is defi nite that the laminar fl uid fl ow and the strong fl uid shear stress induces elongation of endothelial cells and directional stress fi ber organization along with the direction of blood fl ow. On the other hand, endothelial cells applied to disturbed fl ow or low fl uid shear stress showed cobblestone like morphology and exhibit loosely arranged stress fi bers in the cell. Cell shape and cytoskeletal arrangement of endothelial cells is highly dependent of the magnitude of fl uid shear stress. It is well known that the endothelial cells at the atherosclerosis lesion shows much reduced endothelial cell polarized morphology. In this area, atherosclerosis develops at the branching site of the aorta showing earliest fatty streaks. At the branching site, endothelial cells suffered high shear stress due to the laminar blood fl ow, thereby protecting them from forming fatty streak organization, and therefore this area is considered to be an atheroprotective region (Berk 2008;Vartanian et al 2008). On the other hand, in the disturbed fl ow area, endothelial cells showed a cobblestone like morphology without any developed stress fi bers. Cell apoptosis are also observed in disturbed fl ow area (Tardy et al 1997;Tricot et al 2000). In many case, atherosclerosis lesions Role of stress fi bers and focal adhesions for mechano-signalling develop in the area where the fl uid shear stress is low, so that the low shear stress area is atherogenic. In this region, the elongation of endothelial cells and the organization of cytoskeletal components, such as stress fi bers and focal adhesions, are highly prohibited. The above observations strongly suggest that the down regulation in the organization of certain cytoskeletal components by low fl uid shear stress seems to be earlier event for the development of atherosclerosis lesions. Endothelial elongation and the distribution of cytoskeletal components are not simply determined by the magnitude fl uid shear stress (Katoh et al 2007b;, 2008Vartanian et al 2008). The cells applied a sustained and uni-directional fl ow in venous endothelial cells showed well elongated morphology, but organized small number of stress fi bers (Katoh et al 2007a). Endothelial cells cultured on micropatterning substrates showed cell shape change independently with fl uid shear stress (Vartanian et al 2008). Thrombin stimulation induces stress fi ber organization and cell elongation in endothelial cells (van Nieuw Amerongen et al 2008). Mechanical cyclic stretch also causes morphological changes and induces cytoskeletal organization (Naruse et al 1998;Kakisis et al 2004). However, at least in in situ arterial endothelial cells, major determinant of endothelial cell morphology seems to be fl uid shear stress.
Our recent study showed direct evidence of a disturbed fl ow at the bifurcation point of the common iliac artery in living animals using an ultrasound micro imaging system (Katoh et al 2007a). Even in the aorta, not all endothelial cells are exposed to the same blood fl ow. Observations of the fl ow pattern in the aorta demonstrated that there are certain areas where a disturbed blood fl ow is generated (Davies et al 2003;VanderLaan et al 2004;Katoh et al 2007a). As we already mentioned, the organization of stress fi bers and focal adhesions changes according to the magnitude of fl uid shear stress in aortic endothelial cells (Davies et al 1994(Davies et al , 2003 (Figure 3). A surgical coarctation made at the straight portion of abdominal aorta, where the artifi cially high shear stress is applied over endothelial cell in in situ, increases the number and size of both stress fi bers and focal adhesions . Even under physiological conditions, the localization and distribution of stress fi bers and focal adhesions seem to be affected by the power of the local fl uid shear stress force (Katoh et al 2007a).
Tyrosine residues of certain proteins involved in signal transduction are phosphorylated when the proteins are functionally activated. The level of activation of tyrosine residues in in situ endothelial cells is a refl ection of the signal transduction of a local portion of the blood vessel. In high shear stress areas, at both the bifurcation point of the artery or a region of surgical coarctation, the expression level of phosphotyrosine containing proteins, such as c-Src and platelet endothelial cell adhesion molecule-1 (PECAM-1) are strictly increased at the cell-cell apposition sites Osawa et al 2002;Katoh et al 2007a). This suggests that the area of cell-cell appositions in endothelial cells, where apical stress are fi bers directly connected, is a 1 site of mechano-signal transduction Osawa et al 2002). PECAM-1 and c-Src are proteins that are tyrosine phosphorylated in a shear stress-dependent manner. Both proteins are observed at the cell-cell apposition sites in in situ endothelial cells, especially in the high shear stress areas at the branching point and a surgical coarctation zone of the aorta. Upregulation of PECAM-1 and c-Src seems to have a key role in the organization of stress fi bers and focal adhesions in aortic endothelial cells. Further experiments are expected to solve this signal-transduction mechanisms in endothelial cells. b a Figure 3 Stress fi bers in in situ endothelial cells in a low blood fl ow area (a) and a high blood fl ow area (b). In the low blood fl ow area, cell shape is circular and the expression of stress fi bers is low (a). In the direction of high blood fl ow, the endothelial cells are elongated and the expression of stress fi bers is increased along the direction of the blood fl ow (b). Note: Bar 20 μm.
In the straight abdominal aorta, the blood fl ow pattern applied over the endothelial cells is considered to be laminar. However, a disturbed fl ow around the branching point is expected to generate atherosclerosis disease. Endothelial cells at the disturbed fl ow in the branching side of blood vessel are exposed to lower shear stress than the straight portion of the aorta. It is possible that the fl uid shear stress generated by the blood fl ow is transmitted directly from the apical plaque (focal adhesion-like structures) to the focal adhesions at the basal side of the endothelial cells and/or from the lateral cell to cell apposition sites via the stress fi bers. This strongly suggests that the apical plaques and their associated stress fi bers are possible sites where signal transduction takes place .
As we mentioned previously, the macromolecular composition of the "apical plaque" is essentially the same as that for the focal adhesions observed at the basal portion of the cell in both in vitro and in situ cell systems. The apical stress fi bers in both cultured and in situ cells are fi rmly anchored to the plasma membrane at the areas of apical plaques with focal adhesion-associated proteins. Using cultured cell systems, other researchers have demonstrated direct evidence that the stress fi bers transmit mechanical stimuli from the outside to the inside of the cell (Hayakawa et al 2008). The cellular structure to transmit mechanical force from the apical to the basal portion of the cell must be relatively stiff and should be easily controlled according to the response to the mechanical stimuli (see the schematic illustration in Figure 4 and Figure 5). The apical plaque and apical stress fi bers may work together as "mechanotransducers" which thus directly transmit a mechanical force applied over the apical surface of the endothelial cell to the internal side of the cell.

Conclusion
The majority of the stress fi bers are located within the basal side of endothelial cells, and both their ends are tightly associated with the focal adhesion. Among the basally located stress fi bers, some stress fi bers are located along the apical plasma membrane of the endothelial cells via specialized attachment sites called "apical plaques". Isometrically contractile stress fibers can form hard connections between apical plaques and focal adhesions located at the basal side of the cells. Such connections have possibility to transmit mechanical force to the focal adhesion and cell-cell adhesion sites where signaling events can be initiated. Since the apical plaques and apical stress fi bers are located at the surface of endothelial cells in situ, they seems to act as transducer of mechanical stimuli generated by the blood fl ow. Apical plaques include the several kinds of signal transduction-associated proteins, such as phosphotyrosine containing proteins, focal adhesion kinase and c-Src, so that the apical plaque and its associated stress fi bers together seem to play