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Ascorbic acid content of human corneal epithelium.

PURPOSE
To measure the concentration of ascorbic acid in the human corneal epithelium.


METHODS
Corneal epithelium was removed from postmortem eyes 4 to 16 hours after death and ascorbate measured by high-performance liquid chromatography.


RESULTS
The concentration of ascorbate was 1.33 +/- 0.48 mg/gm wet weight (mean +/- SD), estimated to be 14 times its concentration in the aqueous humor.


CONCLUSIONS
Ascorbate can protect the basal layer of the epithelium by absorption of incident ultraviolet radiation.