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Identification of Coxiella burnetii RpoS‐dependent Promoters

C. burnetii is an obligate intracellular parasite that undergoes a biphasic life cycle with distinct morphological forms termed large cell variants (LCV) and small cell variants (SCV). 1 Seshadri and colleagues identified C. burnetii RpoS ( σ 38 ), a stationary phase sigma factor, and found the highest level of expression in LCV. 2 C. burnetii RpoS has been hypothesized as mediating the transition of SCV to LCV. 2 In Escherichia coli , RpoS specifically regulates gene expression during entry into stationary phase or when bacteria encounters adverse conditions, such as prolonged starvation, osmotic and oxidative stress, or low pH. RpoS also regulates expression of virulence genes in Salmonella typhimurium , 3 Pseudomonas aeruginosa , 4 Yersinia enterocolitica , 5 and in other pathogens. To further investigate C. burnetii RpoS, we developed a two-vector system that allows identification of C. burnetii RpoS-regulated genes in E. coli .