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Forensic entomology research in Egypt: a review article

Forensic entomology (FE) is widely considered as an important field of forensic science, particularly in the field of estimating postmortem intervals, which has advanced considerably across the world. Researchers used necrophagous insects in twelve Egyptian governorates to look into the variables that impact insect succession and the growth of juvenile stages from infesting animal models and humans, draw toxicological conclusions, and identify insect species using morphological and molecular-based approaches. The study includes analyses of insect abundance and dispersion, along with biochemical and electrophysiological experiments. Comparable data from other Egyptian governorates, on the other side, is currently unavailable. Aspects of FE are also missing, which might be addressed in a future research project. This review aims to provide an outline of FE in Egypt, other than to assist existing researchers in identifying research needs and recruiting new researchers into the field by highlighting significant forensic insects that might be employed in criminal prosecutions.


Background
In forensic entomology (FE), insects are studied for legal purposes, such as in a court of law (Catts & Goff, 1992), to assist in estimating the postmortem interval (PMI) , in cases where corpses are transported from one location to another, and the method and cause of death (Sukontason et al., 2007). Estimating the PMI predicted on the premise that insects land on the corpse soon after death is critical to employing insects as a resource in court proceedings, according to Amendt et al. (2007). As a corpse decomposes, flies are drawn to the odors produced by gases and biological fluids leaking from natural body holes, as well as blood escaping from wounds, generating a variety of microhabitats, each of which attracts its own species of insects (Lord & Rodriguez, 1989). By examining the development and/or succession patterns (SPs) of insects found on human remains, forensic entomologists can estimate the PMI (Goff, 1993). Many factors were found to impact on the SPs such as season, location, and microclimate (Lord & Rodriguez, 1989;Byrd & Sutton, 2020). Another application of FE is the detection of chemicals in decaying tissues (Nuorteva and Nuorteva, 1982). Furthermore, according to Zhu et al. (2007), entomology is used in criminal investigations in several countries.
Insect identification is one of the most significant techniques in FE. Because only deceased specimens are usually available from the scene, and they are typically in poor condition, morphological identification was mostly utilized. So, DNA methods were utilized to identify insects to solve this problem (Catts & Goff, 1992). Insect species have varying temperature and developmental needs based on their biogeographic zone; therefore, development studies are important (Tabor et al., 2005). Furthermore, the species composition and SPs of a deceased cadaver are affected by the geographical location and season (Anderson 2001, Grassberger &Frank, Mashaly andIbrahim Egyptian Journal of Forensic Sciences (2022) 12:11 2004; Mashaly et al., 2017). Also, when calculating the time before death in another location, evidence from one area should be taken with caution.
According to Zhu et al. (2007), entomology is used in crime investigation in several nations, including murder and other high-profile crimes. The present research investigates FE research in Egypt and the possibility of using entomology in criminal matters.

Collecting data
Using Google Scholar, a past working of the current published literature on various aspects of FE and its legal implications in Egypt was conducted. Additionally, numerous related Web pages were accessed. To find information in the literature, the following search keywords were employed individually and in combination: "Forensic Entomology, " "Insect succession, " " DNA Barcoding in Insects, " "Insect development, " "Entomotoxicology, " and "Egypt. " When writing this review paper, publications regarding insects that did not have a forensic focus were excluded. This study included and examined 55 peer-reviewed academic publications.

Study locations
Egypt is a transcontinental country that extends from Africa's northeastern corner to Asia's southwest corner, spanning the Sinai Peninsula. There are also 27 governorates in Egypt (Fig. 1). Egypt is located between 22 and 36° 31° north latitude, with the cancer's orbit crossing over Aswan and lying between 24 and 37° east of Greenwich. Egypt has an arid desert environment throughout, except for a tiny stretch of Mediterranean shoreline. Days in Egypt are often warm to hot, and evenings are chilly. It only has two seasons: a moderate winter (November to April) and a warm summer (May to October). During the summer, temperatures throughout almost the whole country reach 30 °C (86 °F) during the day and (much) higher at night. Temperatures as high as 40 °C (104 °F) in the shade are not uncommon in the season. The annual precipitation in most parts of Egypt is less than 80 mm (3.14 inches). Even though most of the rain falls along the coast, the wettest area (around Alexandria) only receives about 200 mm (7.87 inches) of rain each year.

Carcass decomposition, insect distribution, and succession studies
Over time, the carcass decomposed into well-known decaying stages (Henssge et al., 1995), drawing flies, beetles, ants, and a range of more arthropods (Catts & Haskell, 1990). Temperature, humidity, clothing, burning, and food availability are all elements that impact carrion decay and SPs in each location .
In addition, the PMI may also be estimated by examining arthropod SPs and stages of body decay (Smith 1986;Voss et al., 2011;Morris & Dadour, 2015). In Egypt, research on corpse decomposition and SPs were carried out in eleven governorates that may represent various settings.

Matrouh
In the Western desert of Matrouh governorate, Hegazi et al. (1991) studied the monthly SPs of insects on the flesh of a mature snail and a small carp in two habitats (above and below the ground). Nine insect families were discovered, with 19 insect species belonging to the three orders of flies, beetles, and ants. Insects appeared to like fish baits somewhat more than snail baits. During the study period, flies were the most frequent insects on both baits, with the Calliphorid family being the most common. Diptera seemed to consume aboveground carrion, whereas Histerids and Dermestids beetles were found both above and below the ground surface. According to this study, the SPs of insects differed dependent on the carcass type and habitat.

Alexandria
Rabbits were employed as experimental animals in six studies. Seasonal fluctuations, as well as the reasons of mortality and the stage of decomposition (El-Kady et al., 1994a, EL-Kady et al., 1994b, have been found to influence the decaying process and SPs . Tantawi et al. (1998) also looked at the fly larvae SPs on rabbit carcasses subjected at the same time and in the same place from three groups, blowflies, flesh flies, and house flies. The other two experiments looked at the impacts of propoxur (Abdel  and amitriptyline (Abdel . . The presence of such chemicals impacted the duration of decay stages, SPs, and the rate at which their immature stages grew, according to the researchers. Researchers in Alexandria observed that insect SPs were impacted by mortality reasons, seasonal changes, and the existence of chemicals. Suez Gabre (2002) and  conducted a seasonal field research using four bait traps (fish, bovine-lung, grapes, and molasses) to characterize the abundance levels, pattern, and sex ratio of carrion flies. The most abundant flies found during the trial were Lucilia cuprina Wiedemann, Chrysomya megacephala Fabricius, and Musca domestica Linnaeus. Furthermore, the almost identical number of male and female flies captured on grape and molasses baits might represent the real male-female population balance. Female flies were also found in far greater numbers on fish and bovine-lung baits than on grape or molasses baits. Lastly, the number, distribution, and sex ratio of carrion flies were all impacted by the bait type, according to the two studies in Suez.

El-Qalyubiya
In the Governorate, two studies were included. Abd EL-Bar and Sawaby (2011) explored the role of pirimiphos-methyl, an organophosphate pesticide, on the process of decay and insect attractiveness. They observed that whereas the bodies of control rabbits decomposed quickly, the carcasses of treated rabbits did not, even after 40 days. The same group of insects invaded the guinea pig and rabbit carcasses, with Chrysomya albiceps Wiedemann being the first to arrive. On the treated carcasses, there were less blowfly larvae, which might explain the unusual decaying delay. Formicidae were noticed in all dead bodies, particularly in the early stages   of decay. Ibrahim et al. (2013) discovered that guinea pig corpses decayed quickly in the fall and summer, that insect attraction followed a predictable seasonal pattern, and that the insect colony grew in richness as the decay continued. Seasonal and decaying stage differences, or the existence of chemicals, all influence the insect exposed to carcasses, according to the two experiments in El-Qalyubiya.

Ismailia
Aly et al. (2013) conducted a research in Ismailia, where they examined SPs and identified attracted species on rabbit and rat remains during the summer. Four fly families, two beetle families, and one ant species (Monomorium minimum Buckley) were among the insects gathered. Finally, the research showed that the decay rate and the number of visitor species varied depending on the decay stage.

Cairo
In Cairo, six studies were conducted, four of which looked at the effects of seasonal and habitat change and two of which looked at the effects of chemicals. Using rabbits and dogs as animal models, Zeariy et al. (2015,2018) and Zeariya and Kabadaia (2019a, b) examined the different influences of carcass decomposing stages, carcass type, and habitats (indoor and outdoor) on insect diversity and SPs. According to the studies, the blowfly, Ch. albiceps, was the first fly driven to the initial days of decaying. Likewise, the insect variety on the two carcasses looked to be rising outdoors and decreasing indoors, with larger numbers seen on dog carcasses than rabbit carcasses. Also impacted by the decaying stage are the amount and variety of insects. In particular, the warmer seasons had the shortest larval and pupal lengths, whereas the cold seasons saw the longest larval and pupal lengths. Zinc phosphide-intoxicated  and warfarin  chemicals have been studied for their influence on SPs and decomposing processes. Control carrions decomposed quicker than treated corpses, according to , whereas treated carcasses decomposed faster than controls, according to . In addition, control carcasses attracted more insects than zinc phosphide-intoxicated treated carcasses, but warfarin carcasses attracted much more insects than control bodies. Furthermore,  remarked that the rabbit fur color influences insect attractiveness, with black rabbit remains attracting more adults than other colors.
According to the studies conducted in Cairo, changes in carcass type and habitat impact the sorts of insects involved and the SPs. Additionally, the occurrence of chemicals influenced the decay rate and the attractiveness of insects to carcasses.

Fayoum
The effect of tramadol on rat carcass decomposition, SPs, and rate of growth of Sarcophaga argyrostoma Robineau-Desvoidy was investigated in a study by . According to the research, the decomposition of the treated carcass was not substantially quicker than that of control carcasses. Chrysomya albiceps, L. cuprina, and M. domestica were fewer in treated carcasses than control carcasses. Female Sarcophaga spp., in contrast, were more drawn to the treated ones. Sarcophaga argyrostoma maggots collected from treated groups were much longer than that collected from control groups, and maggots fed treated groups pupated 2 days later than maggots fed control groups. As a result, tramadol had no effect on carcass decay, but it did affect the insect appeal to carcasses.

Qena
Aly et al. (2017) did a research in Qena that looked at the insects that were associated to rabbit carcasses in both outdoor and indoor settings. They found that, in comparison to winter and fall, the rate of body decomposition was quicker in summer and spring. Outdoor bodies decay more quickly than indoor bodies. Outdoor bodies decay more quickly than indoor bodies. There was a total of 18 species identified, including 5 in the outdoors, 3 in the indoors, and 10 common species among them. Twelve Diptera, three Coleoptera, and three Hymenoptera species were reported. Blowflies and flesh flies were also the earliest to appear on rabbit carcasses. According to the authors, variations in decomposing stages, place, and seasonality impacted insect types and succession tendencies.

Assiut
Abd El-Aziz and El Shehaby (2019) analyzed the forensic arthropod species that colonizes rat carrions in an aerated habitat throughout the spring season. Consequently, the fauna consists of 13 species of insects from 10 families, with blowflies being the first to arrive, followed by other flies in various stages of decay, and beetles limited to the late stages. Finally, the date of the initial insect appearance and the insect type varies depending on the decay stage.

Giza
The effects of clothes on the decomposition and insect attractiveness to rabbit carcasses were investigated by . They discovered that clothes considerably slowed the decay rate and that the existence of clothes had no impact on the insect species drawn to the carcass or the SPs recorded.

Sharqia
During the summer season, Farag et al. (2021) looked at the variety and SPs of insects that colonized indoor rat carrion killed by various methods (cervical displacement, electrocution, immersion, or slaughtering). The most prevalent insects discovered were flies and beetles, with blowflies being the first to occupy the carrion and lasting until the start of the dry stage. In addition, neither the SPs nor the number of related insects varied between the control and immersion groups. There was, however, a distinction between the electrocuted and slaughtered groups.

Insect growth and development studies
It is crucial to know which insects are attracted to dead bodies in a specific region, as well as the growth rates of prominent carrion insect larvae, when estimating PMI using insect data (Gennard 2007). According to Tarone and Benoit (2019), the location temperature data is critical for complete entomological investigation since insects have temperature-dependent life cycles. Still, temperature is the extremely considerable piece of data applied to PMI estimation based on the growth rates of insects, as per Byrd and Sutton (2020).
Nine developmental experiments were brought in six governorates, focusing on five flies: four blowflies and one flesh fly. El-Shazly et al. (1995) compared the developing and propagative characteristics of Ch. albiceps and Parasarcophaga argyrostoma, then age-specific survival and productiveness. They noticed that the two flies are on opposite ends of the r-strategist spectrum. Kheirallah et al. (2006Kheirallah et al. ( , 2007 investigated the temperature effects on the growth of Lucilia sericata Meigen and Ch. albiceps using four different temperatures. According to the studies, Ch. albiceps has a greater growth level and a longer larval development time than L. sericata. Moreover, it was discovered that in pure and mixed cultures of varied densities, Ch. albiceps development was unaffected when L. sericata density grew while Ch. albiceps density stayed constant. Likewise, the sex ratio of both species developing in pure and mixed cultures was unaffected by egg density or temperature, and greater extents resulted in lower adult sizes for both species. Rashed et al. (2015) used Ch. albiceps to probe the impact of temperature changes on growth rate in distinct seasons. They observed that the total development period of the blowflies was affected by temperature fluctuations and showed substantial seasonal variability. High temperatures promote the growth of the fly, but low temperatures slow it down. Females emerged at higher rates than males, and the female ratio was greater throughout the winter.  also looked at how the rodenticide warfarin influenced the growth and survival of fly maggots (L. sericata, Ch. albiceps, and Ch. megacephala). They discovered that treated larvae grew faster than control larvae, and seasonal temperature fluctuation is a crucial element in the blowflies' growth time. Gabre et al. (2005) investigated the Ch. megacephala life cycle in the lab at a temperature of 26 °C, where the two-sex life table assessment offers a thorough explanation of the fly developmental stage differentiation. Ibrahim et al. (2014) also investigated the effect of larval density on the fly population ecology and survival. They observed that there is a link between larval density and survival, that density affects pre-oviposition time but not sex ratio, and that density influences fertility. AbouZied et al. (2003) used an age-stage, two-sex life table, and different growth rates among individuals to evaluate raw data on L. cuprina life history in a lab setting. The outcomes showed that the L. cuprina population is r-strategist. Also, Elshehaby et al. (2016) looked at the impacts of three various types of feed (liver, beef, and meat) on the number of L. sericata and their growth time. They determined that the development rate of all stages of cultured flies increases when the kind of meat diet changes, flies prefer meat other than liver and beef, and the development characteristics of L. sericata were substantially higher with diet meat than with the other diets examined. As a result, the authors concluded that diet meat may be used to mass rear blowfly larvae for a variety of fundamental and applied research projects.
According to the developmental research, temperature is the most essential element influencing the flies' life cycle, with a substantial influence on pre-oviposition and incubation periods, egg hatching percentage, female fecundity, and larval growth time.

Insect identification
The precise and rapid classification of insects is a first and essential stage in using insect proof in forensics. According to Tang et al. (2012), traditional morphological insect identification poses a considerable challenge for forensic scientists, but genetic approaches for insect identification are faster and more efficient. In Egypt, Tantawi and El-Kady (1997) used morphological methods to distinguish third-instar larvae of various flies from blowflies, flesh flies, and house flies, while Sawaby et al. (2016) identified 29 species from 9 families in a taxonomic study on beetles, and Sawaby et al. (2018) used the same characteristics to identify dipterous species.
A few articles, instead, used molecular identification, such as in a master research by , data assessment utilizing the RAPD polymerase chain reaction technology proved the third larval instar of Sarcophaga carnaria Linnaeus. Aly (2014) obtained from Aswan and Benha. The study found variations in genome size within and between the juvenile stages of the two species. Finally, the forensic insects were identified using both morphological and molecular methods in the classification experiments.

Entomotoxicology
Entomotoxicology is commonly applied when toxicological tests on bodily tissues are not possible (Verma & Paul, 2013), or when internal organs, blood, or urine are not available for a criminal probe, entomotoxicology is frequently used (Sankhla et al., 2017). Several studies in Egypt employed it to detect the existence of chemicals in insect larvae and body tissues, as well as their effects on larval growth. Tantawi et al. (2001) utilized a radioimmunoassay technique to measure the level of morphine in the 3rd instar larvae of blowflies and flesh flies attracted to rabbit carcasses left outdoors. El-Samad et al. (2011) employed high-performing liquid chromatography with a UV-visible spectrophotometric detector to identify tramadol in rabbit blood and tissues, along with fly larvae, to assess tramadol's impact on the growth of L. sericata larvae. According to the research, tramadol concentrations in larvae were much lower than those detected in tissues, treated rabbits reached maximum size faster than control rabbits, and the overall developing period rose significantly when the first administered dose was increased.
Malathion was noticed in Ch. albiceps maggots and tissues of treated dog carcasses in a research by Fouda et al. (2017a), where it dramatically changed the durations of developing stages of flies inhabiting the treated carcass but had no effect on the insect number drawn to the carcasses. Fouda et al. (2017b) were also capable to detect ephedrine in Ch. albiceps larvae, and the influence of ephedrine on the growth of maggots. In addition, Tahoun and Abouzied (2017) used GC-Ms analysis to detect tramadol in S. argyrostoma maggots and investigate the influence of tramadol accumulation on maggots and pupae development rates. They observed that tramadol accumulation decreased the maggots and pupae lifespans. When maggots were fed tramadol-treated livers, the average weight of the 3rd instar larvae decreased while the pupae weight rose. Bakr et al. (2018) looked at the impact of malathion on Ch. megacephala larval development, and the consequences for PMI estimation. Malathion was detected in all treated rabbit tissues, but not in the controls, it was detected in the lungs in the highest intensities, followed by muscle tissue and lipids, as well as in all 3rd larval instars and pupae.
The impacts of morphine on the growth in four flies, L. sericata, Ch. albiceps, Ch. megacephala, and S. argyrostoma, were studied by El-Samad et al. (2020). The drug was found in both blood and samples of tissue taken from treated rabbits, albeit the amounts of morphine found in larval samples for all fly species were lower than in rabbit tissues. Abd El-Gawad et al. (2020) looked at the possibility of using fly larvae as diagnostic indicators for warfarin toxicity. Maggots exhibited two distinct results: positive, which may be owing to the larvae eating on the rabbit liver, and negative, which could be owing to the toxin being highly destroyed after 18 hours of ingestion.
In general, forensic entomotoxicology was applied by numerous Egyptian researchers to check for drugs like tramadol, malathion, morphine, ephedrine, and warfarin in insect larvae and carcass tissues.

Biochemical studies
Biochemistry is the analysis of biological processes at the cellular and molecular level using chemicals. Sawaby et al. (2009) conducted the only study in Egypt to explore Page 8 of 15 Mashaly and Ibrahim Egyptian Journal of Forensic Sciences (2022) 12:11 how the pesticide pirimiphos-methyl changes the protein composition of insects drawn to treated rabbit carrion, with the insecticide's effect on insects. According to the research results, Ch. albiceps and Dermestis maculatus DeGeer contain a functional esterase system. The value of entomological techniques for detecting pharmaceuticals in insects that infested drug-death suspect carcasses, for instance protein profiles and esterase assays, which are involved in physiological and endogenous metabolic processes, is highlighted in this work.

Electrophysiology
Electrophysiology is a subfield of physiology that studies the electrical properties of biological cells and tissues. There have been several probes of chemosensory sensilla in a variety of insect families, but there is comparatively little information about forensic fly species. Refai et al. (2009) investigated the electrical reactions of receptor nerve cell on the antennae, labium, and tarsus of Calliphora vicina Robineau-Desvoidy to various quantities of morphine in decaying tissues alongside odors originating from under the surface. The 5th cell "deterrent cell" was shown to be the best responsive cell to morphine sulfate at all sites of stimulation, although with varying levels of sensitivity. These findings clearly imply that varying dosages of morphine sulfate have a great repellant impact on C. vicina attraction. Also, to investigate if morphine sulfate has a repellant impact on blowfly attraction to corpses, Abdel  conducted electrophysiological and behavioral studies. They realized that the drug stimulated the fifth cell in the chemosensillae, causing a delay in blowfly attraction to treated carrions compared to control carrions. Researchers concluded that entomologists must be conscious of the delay in adult fly arrival to the carrion in cases where the victim died because of morphine abuse, which could lead to a misinterpretation of PMI estimation, especially when previous studies were showed on morphine-free experimental carcasses.

Human case study
Forensic entomology, according to Hall (1990), concentrates on insect-infested human cadavers. Insects may represent crucial tools in criminal investigations, in addition to their biological significance (Catts, 1992). Insects can be designed to estimate the time since death, especially in the case of putrefied corpses (Singh, A. et al. 2019). Insects can also aid when typical PMI estimation methods based on early postmortem phenomena like body cooling, livor mortis, and rigor mortis fail (Saukko & Knight, 2004). Several experts have studied the insects that are drawn to human remains in different countries (Lee, 1989;Barreto et al., 2002;Lira et al., 2020, Mashaly et al., 2020. Also, no insect attraction to human cases has been studied in the regions around Egypt, except for Saudi Arabia (Al-Qahtni et al., 2019. In Egypt, a few studies on human cases were done to document the drawn insects to the bodies and attempt to apply this knowledge in estimating the PMI. Galal et al. (2009) investigated the insect fauna and SPs on degraded human leftover parts in Assiut during the summer and spring. Flesh flies, blowflies, house flies, skin beetles, and ants were all found, with flies being the highly frequent insects and Ch. albiceps being the extremely prevalent species. Moreover, El-Mehy et al. (2015) investigated the ability of insects to estimate PMI in 15 human cases in Gharbia (11 men and 4 females). Nine cases were found in open habitats, three in aquatic environments, and three in subterranean habitats, with ages ranging from 7 to 50 years. In the 15 carcasses, blowflies and flesh fly maggots were found, with Ch. albiceps being the most common (9 instances) and Sarcophaga species following closely behind (4 cases). In two cases killed by carbamate and organophosphorus poisoning, toxicological investigations using larvae attached to decaying remains were helpful. Besides, Tantawi et al. (2018) collected insects during an autopsy of 15 human bodies at El-Esaaf Mortuary in Kom El-Deka, Alexandria. Six fly species maggots from three families were gathered from the remains, as these flies were the first to occupy the remains and hence are crucial in PMI estimating in Alexandria. Blowfly maggots were the highly frequent and numerous insects in all times and environments, with Ch. albiceps being the extremely common species; however, in warmer seasons, S. argyrostoma was revealed to be a very indicative species.
Three studies on human cases were conducted in Egypt, in three distinct governorates, to recording the drawn insects to the dead corpses and PMI estimation.

Forensically important insects in Egypt
From the 55 studies in Egypt, twenty insect species (Table 2) belonging to 3 orders were selected as the most significant and common species. There were 11 fly species, including 5 Calliphorids, 4 Sarcophagids, and 2 Muscids. The order Coleoptera had four species, two Dermestids, one Histerid, and one Clerid, whereas the order Hymenoptera included five Formicides. Among the 20 common species analyzed, Ch. albiceps, Ch. megacephala, L. sericata, M. domestica, and D. maculatus were the leading species of corpses community structure. Chrysomya albiceps was the highly often introduced species in 11 governorates and 35 articles, followed by L. sericata verified in 7 governorates and 21 articles, and Ch. megacephala introduced in 6 governorates and 18 articles. From Muscidae, M. domestica was introduced in Page 9 of 15 Mashaly and Ibrahim Egyptian Journal of Forensic Sciences (2022) 12:11 -Kady et al. (1994); El-Shazly et al. (1995);Tantawi et al. (1996Tantawi et al. ( , 2018; ; Kheirallah et al. (2006Kheirallah et al. ( , 2007; Abdel -Ghaffar et al. Mashaly and Ibrahim Egyptian Journal of Forensic Sciences (2022) 12:11 (2003)  In general, 20 insect species were identified as frequent in the research, with 5 being named the most frequent, and Ch. albiceps looks to be the common insect in all locations.

Animals used in the research
According to Catts and Goff (1992), the animal model must closely resemble the pattern of human body decomposition, be reasonably easy to get, be affordable, and not generate public outcry. One area where the legality of baseline data obtained through decomposition research is commonly questioned is the sort of non-human model used. Various animal models were used in experiments of arthropod SPs on decomposing carcasses, including lizards (Cornaby, 1974); elephants (Coe, 1978); sheep (Fuller, 1934); dogs (Boulkenafet et al., 2015); domestic cats (Early & Goff, 1986); fishes and pigeons (Omer, 2014); camel, goat, and pigs (Tabor et al., 2005); dogs (Mashaly et al., 2019); and rabbits (Al-Mesbah et al. (2012); Mashaly et al. (2017). In Egypt, several animals were used in the investigations, with the rabbit being the most used animal in 30 of them, followed by rats in three. Each of the animals, including the fish, guinea pig, and dog, took part in only one research. Three experiments used rabbits and dogs as comparative animals, while two others used rabbits and guinea pigs. Additionally, rabbits and rats were utilized in one research, while mature snail meat and a small carp were used in another.

Review articles published by Egyptian scientists
A review article can support to know the level of research, address evident contradictions, uncover research gaps, and even build consensus where none previously existed. Several Egyptian scholars produced review articles in the field of FE. El-Kady (1999) wrote an article concerning the complications in using FE, in which he discussed a number of topics, including animals as models for human corpses, replicated carcasses, way of death, nocturnal oviposition of flies, species identifications, maggot age estimation, and foreign chemicals as a cause of error. He concluded that such issues are among the methodological challenges that the entomology expert is likely to face when using insects to PMI estimation and that FE researchers must follow strict guidelines, in adding to develop a better considerate of the ecologies of species allied with decaying bodies. Likewise, Rasmy (2007) discusses how insects and acarines may give crucial information in homicide investigations, as well as a number of criminal cases, including the effective use of entomological data in murder investigations. Rasmy (2008) also made a brief statement about the use of mites and insects as proof of physical abuse. Then, Shalaby (2018) released a brief statement about the possibility of employing insects as witnesses to man's aggressive approaches in forensic research in Egypt. As well as FE definitions, history, carrion decomposition phases, and related arthropods, importance in resolving crimes, techniques for obtaining entomological proof at death sites, and research projects undertaken in Egypt were all addressed by Kenawy and Abdel-Hamid (2019). Ultimately, El-Bassiony (2020) identified a range of blowfly SPs on Egyptian mammalian carrions. It also examined at how regional weather situations influenced the life cycle of L. cuprina, a common fly that survives in cadavers after death.
In general, six review papers were published in Egypt, attempting to cover the major issues of the FE, the difficulties in applying the field, and various data about common insects.

Discussion
The topic of FE is constantly developing, and developmental and distribution data on key species are still understudied in this field, even though it has been in use for a long time. Although the number of academic articles issued in the field of FE, as well as the number of individuals who practice it, more study is needed to find answers to unsolved issues.
In Egypt, FE's importance in the present scientific community is rising, with 7 articles published between 1990 and 1999, 11 papers published between 2000 and 2010, and 37 papers issued between 2011 and 2021. The study appeared that FE researchers in Egypt were interested in the research of seasonal SPs, entomotoxicology, and insect development. Other aspects of FE, for example, a lack of study in several governorates, aquatic and coastal habitats, the impact of different variables such as burning and burial, human cadaver studies, and additional studies on beetles and ants, are also missing. Although FE research using a molecular approach began in the prior decade, it has failed to make significant progress in Page 13 of 15 Mashaly and Ibrahim Egyptian Journal of Forensic Sciences (2022) 12:11 Egypt. Furthermore, most of the experiments have been conducted on blowflies and flesh flies, with very little on house flies. Because Ch. albiceps is the highly reported species in Egypt's studies, it deserves special attention.

Suggestions
Egyptian academics should pay a larger focus on FE studies in the future. Still, an Egyptian Society for FE should be created to facilitate communication and collaboration among Egyptian scientists.

Conclusions
This review's purpose is to create a general overview of Egyptian FE. In Egypt, 55 studies were carried out over 12 governorates. Insect SPs, developmental and toxicological studies, and insect identification were all studied using an assortment of animal models, with some human case studies. Likewise, the study showed a shortage of research in some governorates, with some essential components of FE work, like the impact of varied environmental conditions on corpse decay and SPs. There is also a need to interact with legal medicine to work on more human bodies, which will facilitate to expand FE in Egypt and open the way for its use in criminal probes.