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Preliminary characterization of the histamine releasing activity of cotton dust

and it was mainly in the supernatant fraction. This was confirmed by a final experiment in which the 1000 g and supernatant fractions were incubated together with S-adenosyl-Lmethionine (methyl I4C) and norlaudanosoline followed by extraction of the total alkaloids from both fractions and subsequent autoradiography after development by t.1.c. using acetone-tolueneeethanol-ammonia (conc.) (10:10:3 :+) (Antoun, 1974). Radioactive reticuline, codeine, thebaine and papaverine were found. Narcotine was not present indicating either that the enzymes involved in its formation were not active at the time of collection of latex or that its biosynthesis occurs outside the laticiferous vessels. This preliminary work has shown that poppy latex was capable not only of carrying out the necessary methylation reactions of the alkaloids, but also capable of demethylating thebaine to codeine and possibly morphine.