{'fraction_non_alphanumeric': 0.03782046991097128, 'fraction_numerical': 0.020550237633041035, 'mean_word_length': 4.8611220086308355, 'pattern_counts': {'":': 0, '<': 12, '<?xml version=': 0, '>': 7, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Application of blood pre-albumin and NT-pro BNP levels in evaluating prognosis of elderly chronic heart failure patients

Levels of blood pre-albumin (PA) and N-terminal pro-B-type natriuretic peptide (NT-pro BNP) in elderly patients with chronic heart failure (CHF) and their clinical value in prognosis evaluation were explored. A total of 410 elderly patients aged ≥65 years hospitalized for CHF were enrolled. The concentrations of blood PA and NT-pro BNP, routine blood test and biochemistry indicators were determined and color Doppler echocardiography was performed. Additionally, the patients were followed up after discharge, and based on the occurrence of major adverse cardiac events (MACE), they were divided into MACE group and non-MACE group. MACE group had an older age and a higher level of plasma NT-pro BNP than non-MACE group (77.82±6.57) years vs. (76.39±6.18) years, and (8,864.52±9,718.36) pg/ml vs. (4,165.62+6,437.28) pg/ml (P<0.05), and the left ventricular ejection fraction and serum PA level in MACE group were lower than those in non-MACE group (P<0.05). According to the results of multivariate regression analysis, serum PA [odds ratio (OR)=0.242, 95% confidence interval (CI)=0.137-0.406, P<0.001] and plasma NT-pro BNP (OR=1.847, 95% CI=1.024-3.158, P=0.036) were independent risk factors for the occurrence of cardiac events during follow-up. Decline in PA level and elevation in NT-pro BNP level have a strong correlation with poor prognosis of elderly CHF patients, and they can be used for clinically evaluating disease conditions, guiding treatment and improving prognosis.


Introduction
Chronic heart failure (CHF) is a chronic disease, in which myocardial structure and function are changed due to cardiomyopathy, inflammation, myocardial infarction and other causes, ultimately leading to decline in ventricular pump function (1,2). CHF occurs in the terminal stage of various heart diseases and is a major cause of death in heart disease patients, seriously endangering the life of patients. With the aging of populations and improvement of life expectancy in heart disease patients, CHF have become an important public health concern. Some studies have manifested that the incidence rate of CHF in the Chinese people aged ≥65 years old is as high as 10%, and elderly CHF patients tend to be complicated with malnutrition (3). With a half-life of about 2 days, pre-albumin (PA) is a sensitive indicator for monitoring nutritional status, and the decrease in PA level may be correlated with the poor prognosis of the patients with cardiovascular system disease, without related studies on its role in evaluating the prognosis of CHF (4)(5)(6). N-terminal pro-B-type natriuretic peptide (NT-pro BNP) acts as a common indicator for the diagnosis of acute and CHF and the evaluation of their severity and has a significant correlation with left ventricular ejection fraction (LVEF), but it is more closely associated with age, volume load, renal function and diet habit (7,8). The present study explored the significance of blood PA and NT-pro BNP for disease progression and prognosis in elderly CHF patients to accumulate data for clinical research of CHF.

Subjects.
A total of 410 elderly patients aged ≥65 years admitted to Beijing Chaoyang Hospital (Beijing, China) for CHF from January 2011 to June 2014 were enrolled in this study. There were 194 males and 216 females with the mean age of (77.18±6.34) years. The patients were diagnosed with CHF based on the Framingham Criteria for Congestive Heart Failure, and their cardiac function was classified into grades II-IV (41 cases of grade II, 146 cases of grade III and 223 cases of grade IV) according to the New York Heart Association (NYHA) cardiac function classification standards. Exclusion criteria: i) Patients with malignant tumors, severe infection or tuberculosis, severe liver or kidney disease, thyroid disease, severe malnutrition or acute myocardial infarction, ii) those suffering from severe dehydration, gastrointestinal hemorrhage, shock or hypovolemia and iii) those who underwent major surgical operations in the last 3 months.
This study was approved by the Ethics Committee of Beijing Chaoyang Hospital. Patients who participated in this research had complete clinical data. The signed informed consents were obtained from the patients or the guardians.
General information. The general information of the subjects was collected, including the basic information of age, sex, routine blood test results, blood biochemical analysis results, echocardiograms and hospital stay. On the second day of admission, fasting venous blood was drawn to determine the serum PA and plasma NT-pro BNP concentrations and routine blood test and biochemistry indicators. The blood biochemistry indicators and PA were determined using the automatic biochemistry analyzer (Beckman Coulter, Inc.), and NT-pro BNP was measured using Elecsys 2010 fully automated electrochemiluminescence immunoassay analyzer (Roche Diagnostics). Echocardiography was performed in patients with Sonos 5500 color Doppler ultrasound system (Philips Medical Systems B.V.) within 3 days after admission. The probe frequency is 2-4 mhz. The machine is equipped with harmonic imaging technology (HI) and AQi technology analysis system. All the patients LVESD, LVEDD and other indicators were measured, and the LVEF indicator was calculated by Simpson's method.
Treatment and follow-up. All the patients received standardized treatment based on the '2016 ESC Guidelines for the Diagnosis and Treatment of Acute and Chronic Heart Failure'. After discharge, they were followed up by telephone or outpatient visit, and the follow-up lasted for 14-60 months with the occurrence of major adverse cardiac events (MACE) as the end of follow-up observation. The patients were divided into MACE group and non-MACE group according to the occurrence of MACE that is defined as re-hospitalization for heart failure and all-cause death.
Statistical analysis. All statistics were analyzed using SPSS 20.0 (IBM Corp.). The measurement data conforming to the normal distribution were expressed as (mean ± SD), and t-test/z-test was performed for the comparisons between groups. The count data were presented as percentage or rate, and the comparisons between groups were made using Chi-square test. The variables with statistically significant differences were subjected to univariate logistic analysis, and multivariate logistic regression was used to analyze the potential risk factors. The receiver operating characteristics (ROC) curve analysis was used to analyze the diagnosis efficacy. P<0.05 indicates statistical significance.
Results of logistic regression analysis. With the median as the boundary, the variables with statistically significant differences in baseline comparisons were classified into binary variables for univariate logistic regression analysis. According to the analysis results, NYHA cardiac function classification, LVEDD and NT-pro BNP had an OR value >1, and served as risk factors for the occurrence of MACE (P<0.05; Table II), while with the OR values <1, LVEF, ALB, PA, Hb, Hct and TC were protective factors against MACE (P<0.05; Table II). In the multivariate logistic regression analysis, despite of potential affecting factors indicated in Table III (NYHA, LVEDD, LVEF, ALB, Hb, Hct and TC), PA and NT-pro BNP were still statistically significant for the occurrence of MACE (P<0.05; Table III), indicating that the decrease in PA and increase in NT-pro BNP have a strong correlation with chronic heart dysfunction to heighten the risk of such poor prognoses as all-cause death or re-hospitalization in elderly CHF patients.
Comparisons of heart failure severity and prognosis between PA ≤0.16 g/l group and PA >0.16 g/l group. With the median of PA level as the boundary, the patients followed up were assigned into two groups, namely PA ≤0.16 g/l group (n=198) and PA >0.16 g/l group (n=212). PA ≤0.16 g/l group consisted of 98 males and 100 females, aged (78.74±6.73) years on average, while PA >0.16 g/l group was composed of 102 males and 110 females, with the mean age of (76.21±6.16) years. PA ≤0.16 g/l group had an older age and higher plasma NT-pro BNP level and incidence rate of MACE than PA >0.16 g/l group, and the differences were statistically significant (P<0.05; Table IV), and the NYHA cardiac classification grade based on the percentages of cases of grades II-IV in PA ≤0.16 g/l group were lower than that in PA >0.16 g/l group, with statistically significant differences (P<0.05; Table IV).
Efficacy of serum NT-pro BNP and PA in the diagnosis of CHD patients. The sensitivity of NT-pro BNP in the diagnosis of CHF patients is 77.65%, the specificity is 69.12%, and the corresponding best cut-off point is 3,147.83 pg/ ml; the sensitivity of PA in the diagnosis of CHF patients is 84.32%, the specificity is 76.57%, and the corresponding best cut-off point is 0.16 g/l; ROCAUCNT-pro BNP>ROCAUCPA (0.854 vs. 0.784, P<0.05, Table V; Fig. 1).

Discussion
As a complex clinical syndrome in the severe stage of various cardiovascular diseases, CHF has a very high incidence rate in the elderly, and is characterized by recurrent attack, continuous aggravation, multiple hospitalizations and high mortality rate (2). Therefore, early detection and treatment of CHF in the elderly patients is of great significance for improving the survival rate of the patients. In the present study, MACE occurred in 249 cases during follow-up, accounting for 60.73% of the total subjects, and there were 88 cases of all-cause death,  NT-pro BNP is a non-bioactive polypeptide that is secreted by cardiomyocytes and enters the blood circulation through cell stretching. Under normal conditions, NT-pro BNP is mainly derived from atrial tissues, with a lower circulation level, while its level will be obviously raised under such pathophysiological conditions as altered ventricular volume, increased ventricular wall tension and myocardial ischemia and hypoxia. Previous studies have found that CHF patients with different grades of cardiac function of the   NT-pro BNP, N-terminal pro-B-type natriuretic peptide; PA, pre-albumin. NYHA standards exhibit an obvious inter-group difference in NT-pro BNP level, but NT-pro level varies dramatically among individuals and its measurement can be affected by multiple factors such as sex, age and body mass (7)(8)(9)(10). For example, diuretic and vasoactive drugs produce great influences on the level of circulating NT-pro BNP. According to the results of this study, the level of NT-pro BNP was obviously higher in MACE group than that in non-MACE group, and the results of univariate and multivariate regression analysis revealed that the increase in NT-pro BNP level was significantly correlated with the occurrence of MACE in elderly CHF patients, implying that NT-pro BNP level is closely associated with the prognosis of heart failure, and that serum NT-pro BNP level can be monitored to effectively judge the prognosis of CHF. Although NT-pro BNP has a favorable application value in the severity grading and prognosis evaluation of CHF, there is no set threshold of NT-pro BNP for predicting acute onset of CHF, which remains to be further explored.
Previous studies have demonstrated that malnutrition is an independent predictor for adverse cardiac events in CHF patients, and it is closely associated with CHF in the elderly as well. ALB is commonly used as the indicator for monitoring nutrition level, and a lower level of ALB has been found in some studies to serve as a risk factor for the poor prognosis of CHF patients, but with a longer half-life, ALB is not sensitive in reflecting short-term acute malnutrition (11). PA is an indicator for the early detection of malnutrition, and determining the concentration of plasma PA, more sensitive than ALB, has a special diagnostic value for understanding malnutrition, liver and kidney dysfunctions and disease severity (12)(13)(14). One study measured the level of serum PA in patients with renal failure, and the results manifested that it can be used as one of the highly sensitive indicators reflecting the nutrition status and survival rate of renal failure patients. Moreover, serum PA level declines in the patients with AMI and can be used for judging the conditions and prognosis of such patients, and it is also related to the short-term prognosis of patients with cerebral infarction (5). Besides, the study of Lourenço et al (6) revealed that the concentration of serum PA can promptly reflect the nutrition status in organisms, and it declines as cardiac function is weakened. Additionally, the 6 month mortality rate is obviously raised in HR patients with PA <150 mg/l. It was found in the present study that PA ≤0.16 g/l group had higher plasma NT-pro BNP level and incidence rate of MACE, suggesting that low PA level is positively correlated with the severity of HR in the elderly, consistent with the results of the above-mentioned studies. The results of the study of Cabassi et al (15) manifested that PA is more accurate in predicting the death risk of the elderly CHF patients with a higher level of BNP, and it can be used for recognizing the high-risk CHF patients who need nutritional support. In the present study, multivariate regression analysis was performed with the cardiac events as the dependent variable, and it was discovered that decline in PA level is strongly associated with the occurrence of MACE.
PA is more sensitive in evaluating early changes in nutrition level, and it can be used for predicting heart failure probably because worsened HF impairs liver function and raises the secretion of inflammatory factors through the chronic inflammatory process, thereby increasing consumption of PA and decreasing its synthesis, and ultimately reducing PA level. Therefore, PA level is valuable in predicting the prognosis to a certain degree.
Blood PA and NT-pro BNP levels are effective indicators for predicting the occurrence of cardiac events in elderly CHF patients, and they are of great clinical significance for evaluating the severity and prognosis of the disease. However, lack of ECG data and time recording for MACE follow-up is a limitation of present study, time data should be supplemented in the future research to permit time-based analysis and Cox regression. Since CHF is a complex clinical syndrome due to the synergy of multiple factors, and the present study has a relative small sample size, the specific mechanisms of action of changes in blood PA and NT-pro BNP levels in elderly CHF patients remain to be further explored.
In conclusion, the levels of PA and NT-proBNP are significantly related to the severity of heart failure in elderly CHF patients. The detection of PA and NT-proBNP has guiding significance in evaluating the prognosis of elderly patients with CHF.