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A photoelectrochemical sensor based on a reliable basic photoactive matrix possessing good analytical performance for miRNA-21 detection.

The basic photoactive matrixes on transparent electrodes are essential for the performance of photoelectrochemical (PEC) biosensors. Herein, we demonstrate an optimized fabrication strategy toward a reliable ITO/TiO2/AuNP photoanode by sequential deposition of TiO2/Au nanoparticles (Au NPs) on indium tin oxide (ITO) substrates. The identified fabrication conditions include spin-coating tetraisopropyl titanate on ITO slices followed by in situ electrodeposition of Au NPs and finally the thermal annealing treatment. By the conjugation of the thiolated hairpin NH2-DNA sequence and CdTe quantum dots (QDs) onto the thus-prepared photoanodes, a novel PEC sensor for the ultrasensitive detection of miRNA was constructed. The proposed PEC sensor offered advantages including simple structure, storage stability and excellent detection reproducibility as well as sensitivity and specificity toward miRNA-21. Finally, we found that this PEC displayed a broad detection linear range of 1.0 fM to 1.0 nM with a low detection limit of 0.37 fM. This PEC sensor can also excellently discriminate the mismatched miRNA. Moreover, the PEC sensor also showed a satisfactory result in normal human serum sample analysis. These findings emphasized the importance of basic photoactive matrixes for the fabrication of PEC sensors, providing solid fundamental insights for future application of metal oxide substrates for other PEC applications, especially PEC biosensors.