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Constuents from Gymnema sylvestre leaves. V. Isolation and preliminary characterization of the gymnemic acids.

The objectives of this investigation were to isolate and characterize the constituents of gymnemic acid, the antisweet principle of Gymnema sylvestre leaves, and to make them available for further biological testing. Gymnemic acid was found to be a complex mixture of at least nine closely related acidic glycosides. Solvent extraction and chromatography of gymnemic acid resulted in the isolation of gymnemic acids A-D (the major constituents) and V in crystalline form. Acids A-D are glycosides which yield glucuronic acid on hydrolysis while acids C and D also yield glucose. The gymnemic acids isolated in this study are compared to those described in the literature.