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Evaluation and interpretation of grain density autoradiographs by reflectance photometry

The tritium content of nervous tissue sections is calculated from grain‐density of autoradiographs without loss in anatomical information. The calculation is not based on determination of (expected) grain numbers but on photometric measurements (SKphot), interpreted as the result of the function of exposure time (T) and incorporated activity (β): SKphot = f (T, β).