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Sequence‐specific assignment of the backbone 1H‐ and 31P‐nmr lines in a short DNA duplex with homo‐ and heteronuclear correlated spectroscopy

We report the assignment of the backbone 1H‐ and 31P‐nmr lines in the synthetic hexadeoxyribonucleotide pentaphosphate duplex d(GCATGC)2, using double quantum filtered 1H‐1H correlation spectroscopy, 1H observed 1H‐31P heteronuclear correlation spectroscopy, and 31P relayed 1H‐1H correlation spectroscopy. The strategy used enables one to make sequence‐specific resonance assignments without reference to a known or assumed conformation of the DNA fragment.