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Prevalence of chuA gene virulence factor in Escherichia Coli isolated from clinical samples in AL-Diwaniyah province

The current study aimed to investigate multidrug-resistant E.Coli, and (chuA) gene in Es coli which have virulence factors by PCR. Out of 150 urine and stool samples, 122 gram-negative bacteria (60 isolates belong to E.Coli) and 28 gram-positive bacteria were diagnosed by cultural and biochemical characteristics and API 20E System. The susceptibility test is carrying out for sixteen antibiotics drugs by diffusion method, the results showed that higher resistance to aztreonam was (81.8%), ticarcilin/clavulanic acid was (89.09%), carbencillin was (83.6%), piperacillin was (70.9%), nitrofurantoin was (67.26%). Ceftrixon was (61.8%), the most isolates demonstrated low resistance against levofloxacin (21.8%) and amikacin (43.6%). As it turned out from the study, 40 isolates of Escherichia Coli have multiple resistance to antibiotics (MDR). 