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Comparison of alpha toxin gene among different types of Clostridium perfringens

Alpha toxin is found to be produced by all types of Clostridium perfringens. The genes encoding alpha toxin from the available five types of Clostridium perfringens [A I chicken strain), A (rabbit strain), B, C and D were PCR amplified using specific primers and the PCR products were examined on 1.5% (w/v) agarose gel and demonstrated the same bands comparable to the published alpha toxin gene. The PCR products of a toxin gene from the five types were separately sequenced and aligned with all published a toxin genes of Clostridium perfringens. Identities among all studied alpha toxin gene sequences and with the published ones were nearly 96-98%. There are no any significant differences among these nucleotide sequences. It is concluded that a toxin gem; sequences among different types of Clostridium perfringens are similar and highly conserved.