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EFFECTS ON THE OVARIAN FUNCTION OF SUBCUTANOUS IMPLANTS CONTAINING LYNESTRENOL

The fibrinolytic activity of fertilized rat ovum during tubal passage and that of the blastocysts before and at implantation was investigated. Sprague-Dawley rats were used. They were brought up on standard pellets and housed in a room with controlled light and temperature. The ratsweremated in the evening and the presence of a copulation plug the following morning was considered as day 1 of pregnancy. The females were killed on day 1,2,3,4 and 5 of pregnancy and the ovula were obtained by flushing the fallopian tubes with physiological saline. They were then incubated on fibrin slides. Each glass slide was covered with 0.06 ml fibrinogen and 10 pl thrombin. It was spread over an area of 10 cm2 to obtain a film about 0.07 mm thick. The film with the ova was left in a moist chamber at a constant temperature (23°C) for 30 min. After implantation on day 5 and 6 the uterus was taken out and frozen in liquid nitrogen. The site of implantation was histochemically investigated for its fibrinolytic activity with a fibrin slide technique. Fibrinolytic zones around the ova and the blastocysts were seen as clear areas. These areas were measured and the result expressed in square p of lysis. The fibrinolytic activity of the ova was high during its passage through the fallopian tube. The blastocysts showed a decrease in fibrinolytic activity and the site of implantation showed no activity at all. The significance of the high fibrinolytic activity of the ova during tubal passage is probably to contribute to the prevention of premature nidation and the purpose of its decrease in the blastocysts might be to facilitate implantation.