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Demonstration in vitro of delayed hypersensitivity in experimental allergic orchitis in guinea-pigs.

Cells in the peritoneal exudate obtained from guinea-pigs within 7 days of sensitization with testicular homogenate emulsified in complete Freund's adjuvant, showed inhibition of migration when cultured in the presence of the supernatant from the testis homogenate. Simultaneously, the first signs of orchitis were observed. Delayed skin tests became positive only 19 days after sensitization when the orchitis