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Effect of sodium dodecylsulphate on the binding of Lactococcus lactis subsp. lactis T-80 cells with Trp-P1

Hosono and his coworkers (Hosono et al. 1986a, b, 1988a, 6; Hosoda et al. 1992) have investigated the antimutagenicity of lactic acid bacteria, and focused on the binding capacities of lactic acid bacteria with mutagens such as amino acid pyrolysates and iV-methyl-iV'-nitro-IV-nitrosoguanidine. Their results revealed that the intact cells of lactic acid bacteria isolated from 'dadih', an Indonesian fermented milk (Hosono et al. 19906, c), and Mongolian kefir (Hosono et al. 1990a; Tanabe et al. 1991) showed a remarkable capacity to bind to amino acid pyrolysates such as 3amino-l,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-Pl) and 3-amino-l-methyl-5Hpyrido[4,3-b]indole (Trp-P2), accounting for > 97-37% of the binding capacity of such mutagens. Further, cell wall peptidoglycans prepared from such lactic acid bacteria exhibited a high capacity for binding such mutagens (Tanabe et al. 1991). There have been several reports on the binding kinetics of amino acid pyrolysates, but binding mechanisms are not necessarily the same for the different strains examined. For instance, Morotomi & Mutai (1986) reported that the binding mechanism of Trp-P2 to cell walls of a Lactobacillus strain was effected by cation exchange, but Hosono et al. (19886) suggested that the binding mechanism of cell walls of Streptococcus faecalis was not ionic in nature. In the present study, we investigated the effect of SDS on the binding of cells of Lactococcus lactis subsp. lactis T-80 isolated from Mongolian kefir with Trp-Pl, in order to determine the binding mechanism of the intact cells of this strain with TrpPl .