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[Decolorization of methylene blue by bovine spermatozoa and its significance in artificial insemination (author's transly].

The mechanism of action of methylene blue reduction is discussed with special reference to its relationship to the metabolism of bovine spermatozoa. The results of experimental studies of the effect of metabolic inhibitors on decolorization of methylene blue are reported. Attention is also paid to the effect of the addition of methylene blue to semen on the oxygen uptake of bovine spermatozoa. Methods used to determine the decolorization time of methylene blue in artificial insemination are reviewed. One of these methods is described in greater detail. The results of a number of experiments is concerned with the effect of environmental factors on the decolorization times of suspensions of spermatozoa are reported. Finally, the relationship between the decolorization time of methylene blue and a number of other semen characteristics as well as usefulness of this method in artificial insemination are discussed.