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Cloning and prokaryotic expression of tumor antigen MAGE-3

Objective To amplify MAGE 3 gene 3' terminal 657 bp and construct the recombinant prokaryotic plasmid to induce expression 97-314 aa of MAGE 3. Methods Amplified MAGE 3 gene 3' terminal 657 bp from pUC19/MAGE 3 plasmid that included the full length of human MAGE 3 gene by PCR. After sequencing, the gene was cloned into the expression vector pGEX 4T 1 to construct expression plasmid pGEX/MAGE 3. The DH5 α contained the expression plasmid was induced by IPTG. Results The 3' terminal 657 bp of MAGE 3 was amplified by PCR. The sequences was identical with that reported in GenBank. The expression plasmid pGEX/MAGE 3 was successfully constructed. The DH5α contained the plasmid could express a M r 54 000 fusion protein after being induced by IPTG. The yield of the fused protein was 28% of total proteins. Conclusion The expression plasmid pGEX/MAGE 3 was successfully constructed. The 97-314 aa segment of MAGE 3 was expressed successfully, which may lay a foundation for application of the protein in tumor immunotherapy.