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The specific chemical cleavage of pyrophosphate diesters.

A recent model for DNA replication postulates a pyrophosphate diester at the growing point linking the two daughter strands. This stimulated the development of methods for specifically cleaving pyrophosphate diesters leaving phosphodiester groups intact. Three methods have been found for cleaving pyrophosphate diesters. These methods should have applications in polynucleotide synthesis and in the characterization of any phosphate-containing compounds.