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Antifungal activity of the mixture of quaternary alkaloids isolated from Argemone mexicana against some phytopathogenic fungi

The anti-fungal activity of a mixture of quaternary alkaloids of Argemone mexicana was observed at different concentrations (1000, 2000, 3000, 4000 and 5000 μl/ml) against 10 fungi, viz., Ustilago cynodontis, Cercospora cajani, Sphaerotheca sp., Cercospora sp., Alternaria solani, Bipolaris sp., Helminthosporium sp., Curvularia sp., Fusarium udum and Alternaria cajani. Spore germination was inhibited at 2000, 3000, 4000, 5000 μg/ml. Analysis of phenolics by high performance liquid chromatography (HPLC) recorded 11 peaks in the alkaloids but only three could be identified, viz., tannic, caffeic and ferulic acids. The significant efficacy of the alkaloid under in vitro conditions may open the possibility of its use by farmers under field conditions for controlling some crop diseases.