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Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175

Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B‐SC‐I cells caused a specific cytopathic effect upon acute infection of B‐SC‐1 cells. The virus formed visible plaques on B‐SC‐I cell monolayers after 9 to 14 days of incubation at 34°C. and virus can therefore be titrated by plaque assay. Virus could be re‐isolated from plaques of infected cells. which allows the clonal isolation of HAV variants. The stability of a plaquepurified variant of HAV at elevated temperatures exceeded that of poliovirus type 1. but this variant is less stable than previously reported strains of HAV.