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Bacillus stearothermophilus spore suspensions: effect of storage conditions and time on viability and moist heat resistance.

Storage conditions were shown to affect the viability and moist heat resistance of Bacillus stearothermophilus spore suspensions. Refrigerated storage (4 °C) in distilled water did not significantly affect spore viability, but had an adverse effect on moist heat resistance. Freezer storage (–10 °C) and the subsequent thawings and refreezing of the suspensions was shown to have a significant effect on spore viability without a concomitant loss of moist heat resistance in the surviving population. Refrigerated storage (4 °C) in 0.02 M calcium acetate, pH 9.7, stabilized spore resistance without a significant loss of viability for a minimum of two years.