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Photoimmobilization of biomolecules with a scanning near-field optical microscope: functionalization and detection at the submicrometer scale

In view of future generations of biosensors, immobilization of biomolecules with high spatial resolution onto selected materials or transducer surfaces is required. One way to attach the biomolecules is to induce covalent bonds with the substrate using a photochemical effect. High localization in the attachment is obtained when performing the photochemistry in the next field. In this paper, we present patterns of covalently bond proteins, written with near field UV activation. Observation is done in the near field as well, using near field fluorescence microscopy.