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[Genotyping of EsBLs-producing Escherichia coli by pulsed-field gel electrophoresis in nosocomial infection].

OBJECTIVE
Using a genotying method which is accurate and rapid to study the molecular epidemiology of EsBLs-producing Escherichia coli in nosocomial infection.


METHODS
After an analysis of clinical information to ascertain nosocomial infection, EsBLs were detected by phenotype confirmation test and pulsed-field gel electrophoresis (PFGE).


RESULTS
Three EsBLs-producing strains in ICU were verified as epidemiological-related because their dice coefficient index was higher than 95%. The others were verified as non-epidemiological-related since their different lanes were more than 6.


CONCLUSION
PFGE is a perfect molecular typing method to study epidemiolgy and analyze homology.