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Betavulgaris L. Suspension Cultures Permeabilized with Triton X‐100 Retain Cell Viability and Betacyanines Production Ability: A Digital Image Analysis Study

The influence of Triton X‐100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non‐destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X‐100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non‐permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non‐permeabilized cultures.