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Effects of Annexin II Gene Silencing by SiRNA on Apoptosis of Lymphoma Cell Line Jurkat

Objective To investigate the effects of AnnexinⅡ(A2) gene silencing by siRNA on apoptosis of lymphoma cell line Jurkat.Methods A chemically synthesized siRNA duplex targeting to A2,which had been validated to be efficient in downregulation of A2,was transfected to human lymphoma cell line Jurkat.Then transfection efficiency was analyzed by real-time PCR and flow cytometry.Annexin V-FITC/ P I flow cytometric analysis was performed for apoptosis.Results Compared with those in the negative controls,the ratio of cell apoptosis significantly increased(57.47% ±2.10% vs 28.68%±1.21%,P0.05).It showed that Silencing A2 gene by siRNA had induced the Jurkat cell apoptosis(P0.05).Conclusion A2 gene silencing by siRNA can induce the Jurkat cell apoptosis(P0.05).