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SPECTROPHOTOMETRIC METHOD FOR DETERMINING GLYCOGEN CONCENTRATION IN HELMINTHS: INFLUENCE OF AMMONIUM CHLORIDE IN DIFFERENT TEMPERATURE RANGE

Spectrophotometric methods for determining glycogen content are used in studies to quantify the effect of anthelmintics on carbohydrate and energy metabolism of helminths. The most specific of them include the method proposed by Krisman C.R.,
which was later adapted by Danchenko E.O. and Chirkin A.A. to apply in forensic medicine, and Andreyanov O.N. et al. in helminthology. Saturated solutions of calcium chloride and ammonium chloride are used in the procedure. It is known that the solubility of salts depends on temperature. Krisman C.R. studied the effect of calcium chloride on the optical density of the analyzed solutions depending on temperatures, but no detailed studies were made on the role of ammonium chloride. The present paper presents data on the effect of ammonium chloride on the optical density of the analyzed samples under the influence of temperatures in the range of 10–30 oC. This salt introduced to neutralize alkali increases the optical density of the solutions. It is found that ammonium chloride not only neutralizes the decrease in optical density, but also causes its increase as temperatures increase. In salt-free samples, the deviation from the specified glycogen content (0.1 mg) in the range of 10 oC was 12.1%, and with the addition of ammonium chloride, it decreased and varied within 4–8.5%. To improve the accuracy of the analysis, it is recommended to avoid studies in large temperature ranges.