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Time-resolved multichannel imaging of fluorescent objects embedded in turbid media.

We present a multichannel detection technique for three-dimensional imaging of objects embedded in turbid media by using time-resolved fluorescence. By using a streak camera, we can obtain the experimental data in a single measurement. The data, analyzed by means of a triangulation algorithm, provide accurate localization of a fluorescent object for path lengths of up to 120 scattering mean free paths. The results demonstrate the feasibility of combining fluorescence spectroscopy with time-resolved optical tomography for localizing and identifying embedded objects.