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SDS PAGE OF WHOLE CELL PROTEIN OF NEW RHIZOBIAL GROUPS AND 16S rDNA SEQUENCING OF THEIR REPRESENTATIVES

Based on the previous studies on numerical taxonomy and DNA DNA hybridization, additional isolates in each group were studied by using SDS PAGE of whole cell protein. The full length 16S rDNA ( M r≈1.5 kb) of the representative strains in 3 rhizobial groups were sequenced. By comparing these sequences with those of the known type strains, a phylogenetic tree was generated. This tree indicates that the phylogenetic position of strain SH22623 and SH19312 are closely related to R. galegae, A. vitis, A. tumefaciens, A. rubi , but distinct from other known species of Rhizobium. Strain SH2672 belongs to the branch of M. tianshanense, M. huakuii, M. mediterraneum and M. ciceri.