{'fraction_non_alphanumeric': 0.017675050985723997, 'fraction_numerical': 0.004758667573079538, 'mean_word_length': 5.372294372294372, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Solid State Fermentation: Extraction of Enzyme from Fungus and its use in the synthesis of Silver Nanoparticles

Solid State Fermentation technique has been used to obtain crude enzyme from the pure culture of Aspergillus nigerby inoculating a uniform suspension of the fungal spores in autoclaved fermentation media. A six-day long session of its incubationat optimum ambient was followed by the hydration, stirring and macro-filtration to obtain crude extract. After two rounds of its centrifugation at low temperature, it was storedand its microfiltration was executed to acquire cell-free supernatant full of enzymes stemming from the fungus. Once theneutrality of its pH was ensured, it was retained on PDA slants, sub-cultured periodically and preserved at 4 o C.This clear supernatant containing the enzyme extracted from the fungus A. nigerwas characterized using UV-visible spectroscopy. It was alsoused to synthesize silver nanoparticles from two different concentrations of the precursor AgNO3. The enzyme was found to have successfully bio-reduced the silver ions to nanoparticles. Two reaction mixtures and a control sample of the supernatant were incubated for 36 hours at 29±1 oC in dark to avoid any photochemical reaction. After incubation, the color of the reaction mixtures got changed, indicating the formation of silver nanoparticles. These were centrifuged to obtain purified silver nanoparticles, whose characteristics were experimentally examined.