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LACCASE-BASED SCREEN PRINTED ELECTRODE FOR AMPEROMETRIC DETECTION OF PHENOLIC COMPOUNDS

ABSTRACT A laccase-based screen-printed electrode for monitoring phenolic compounds in environmental samples is described. The selectivity and sensitivity of laccase-catalyzed phenol oxidation were combined with the advantages of the screen-printing microfabrication technology. Laccase was immobilized over the screen-printed carbon surface by simultaneous cross-linking and covalent binding methods. Variables relevant to laccase immobilization on the screen-printed electrode surface were examined and optimized. This sensor allowed the convenient quantification of guaiacol and oguaiacol down to 50 and 30 nmol L−1, respectively, and selective measurements in untreated river and groundwater samples. These, and their attractive performance characteristics, are described.