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The post-mortem diagnosis of influenzal infection by fluorescent IgG, IgA and IgM antibody studies on necropsy blood

SUMMARY Necropsy blood from cases diagnosed as dying from influenza A was examined for specific antibody in the IgG, IgA and IgM fractions and a specific diagnosis of recent infection was made if either IgM or IgA antibody and low titres of IgG antibody were found. By these criteria a diagnostic rate of 77% was found in those cases from whom no virus was isolated. The use of infected cell monolayers grown on polytetrafluoroethylene-coated slides gave a simple method of carrying out these antibody assays, and the use of necropsy blood did not require any special methods of transport of specimens to the virus laboratory.