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A study on proliferation and behavior of retinal pigment epithelial cells on purified alginate films.

BACKGROUND AND OBJECTIVES
Alginate, an anionic polysaccharide distributed widely in the cell walls of brown algae, is used in biomedical applications. However, alginate' s performance as a biomaterial, has limited by its several contamination such as endotoxins, proteins and polyphenols.


METHODS AND RESULTS
To overcome this problem, we have developed using modified Korbutt method for alginate purification. After purification, we made alginate films and used for retinal pigment epithelial cell (RPEs) regeneration. ARPE-19 cells were seeded in non-purified and purified alginate films, and then cell viability and proliferation were estimated by MTT assay and RT-PCR was performed to assess specific cell expression. ARPE-19 cell-loaded alginate films were evaluated specific protein expression by through AEC staining and we examined the cell adhesion by scanning electron micro scopy (SEM).


CONCLUSIONS
In this result, ARPE-19 cells in purified alginate films had higher cell proliferative rate and phenotypic expression than those on non-purified alginate films. The results suggest that purified alginate is useful for RPEs regeneration.