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Effect of Metabolic Inhibitors on Pyrogen Production by Rabbit Leukocytes 1

Summary The metabolic inhibitors, actinomycin D, cycloheximide, puromycin dihydrochloride, puromycin aminonucleoside, and p-fluorophenylalanine did not inhibit the release of leukocytic pyrogen whether endotoxin was preincubated with cells for 20 min at 37°C before addition of inhibitor or inhibitor was preincubated with cells for 1 hr before addition of endotoxin. On the other hand, cortisol inhibited release of pyrogen under both experimental conditions. Poly (I): poly(C) was not effective in inducing rabbit leukocytes to produce an endogenous pyrogen.