{'fraction_non_alphanumeric': 0.0440251572327044, 'fraction_numerical': 0.03522012578616352, 'mean_word_length': 4.685714285714286, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
SEPARATION OF BETAXOLOL HYDROCHLORIDE WITH NEW BONDED CELLULOSE CHIRAL STATIONARY PHASE AND DETERMINATION OF THE ENANTIOMERS IN PLASMA BY HPLC

A new liquid chromatographic method was developed for the chiral separation of the enantiomers of betaxolol hydrochloride. The enantiomers were separated by a Chiralpak IB (250 × 4.6 mm, 5 µm) at 30°C and detected at 274 nm, the mobile phase used was n-hexane-ethanol (95/5, V/V), 0.2% diethylamine (DEA), with a flow rate of 0.8 mL · min−1. The enantiomers of betaxolol hydrochloride were separated for the first time with the Chiralpak IB column, and the resolution was 5.26. With the fluorescence detection (λex = 227 nm, λem = 305 nm), the method was applicable for the determination of the enantiomers of betaxolol hydrochloride in rabbit plasma.