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Storage of Leukocyte‐Poor Red Cell Concentrates: Filtration in a Closed System Using a Sterile Connection Device

Abstract. Storage of leukocyte‐poor red cell concentrates (LP‐RCC) was investigated after filtration in a closed system that was assembled using a Sterile Connection Device (SCD). The LP‐RCC were stored for up to 6 weeks following filtration with either 0.9% saline solution (n = 14) or saline‐adenine‐glucose‐mannitol (SAG M) solution (n = 15) to prime and rinse the cellulose acetate filter. The results were compared with the data of nonfiltered buffy‐coat‐poor red cell concentrates (BC‐poor RCC) stored in SAG M solution (n = 10). All LP‐RCC contained less than 106 leukocytes whereas the nonfiltered BC‐poor RCC contained 6751286 × 106 leukocytes at day 1, decreasing to 83±49 × 106 at day 42. Although glucose consumption, lactic acid production and decrease in pH was similar from day 7 through 28 in both groups of LP‐RCC, a significantly steeper decline of ATP values as well as a higher hemolysis and LDH release was observed in the LP‐RCC filtered with saline. During storage of the nonfiltered BC‐poor RCC in SAG M, significantly higher glucose consumption (p<0.01), LDH release (p<0.001), rate of hemolysis (p<0.001) and a lower pH (p<0.001) were found, compared to the filtered units. It is postulated that the leukocytes present in the nonfiltered BC‐poor RCC were responsible for these differences. The ATP values in the SAG‐M‐filtered and nonfiltered BC‐poor RCC in SAG M were comparable. By comparing the ATP levels and values of the filtered RCC and the nonfiltered BC‐poor RCC we conclude that the LP‐RCC can be stored for 35 days if SAG M solution is used to prime and rinse the filter.