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Screening and identification of agarase-producing microbes in sea shellfish gut

Agarase-producing microbes becomes hotspot for research and development on marine resources as agarase is mainly produced by microbes in sea shellfish gut. Agarose was used as the only carbon source. Forty-four strains of agar-degrading bacteria were initially screened from marine shelfish gut. Then 6 strains,which presented bigger transparent circle around colony by Lugosi iodine liquid staining than the rest,were re-screened. Among them,strain A-001 showed the maximum enzyme activity at 13. 01 U·m L- 1by DNS method. For the colony morphology,the strain A-001 was ivory,gram-negative and short rod. Through the dynamic experiment,the strain A-001 showed motility. Based on physiological and biochemical characteristics and 16 Sr DNA sequencing results,strain A-001 was identified as Vibrio alginolyticus.