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Observation of vascularization and protein after implanting porous silk fibroin films in rat.

Porous silk fibroin films can provide an optimal microenvironment for angiogenesis in vivo. Adhesion and migration of human umbilical vein endothelial cells (HUVEC) on porous silk films were observed by confocal laser scanning microscopy. The expression of fibronectin (FN), laminins (LN), intercellular adhesion molecule-l (ICAM-1) and vascular cell adhesion molecule-l (VCAM-1) after implanting Porous Silk Fibroin Films (PSFFs) as grafts for dermis regeneration in rat were studied. FN, LN, ICAM and VCAM may have direct relationships with angiogenesis. The result will help to design excellent PSFFs and study the process and mechanism of angiogenesis.