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Effectiveness of the “Guanidez” disinfectant on the agents of bee infectious diseases in laboratory conditions

The aim of the work is to study the effect of the disinfectant “Guanidez” on bee infectious diseases agents in vitro. Clinical-epizootological and microbiological methods have been used in the work. There were detected high disinfecting properties of the disinfectant “Guanidez” against pathogens of putrefactive diseases of bees. According to the results of the research, the technological regulation for the manufacturing of the preparation was developed: polyhexamethyleneguanidine hydrochloride at a concentration of at least 20.0%, hydrogen peroxide at a concentration of at least 35.0% and dimethylsulfoxide — 0.05 %. Disinfectant “Guanidez” exhibits pronounced bactericidal properties against the following cultures of microorganisms: Paenibacillus larvae (pathogen of American foulbrood), Melissococcus pluton, Paenibacillus alvei (pathogens of European foulbrood). The results of the “Guanidez” test indicate the effectiveness of the combined use of polyhexamethyleneguanidine hydrochloride, hydrogen peroxide and dimethylsulfoxide. The analysis of the results of laboratory tests of the “Guanidez” disinfectant for prophylactic and forced disinfection confirm its effectiveness and expediency of use for the control of putrefactive diseases