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Insulinlike growth factor 1 regulates mRNA levels of osteonectin and pro‐α1(I)‐collagen in clonal preosteoblastic calvarial cells

A nontransformed rat clonal cell line (UMR‐201) with phenotypic characteristics of osteoblastic precursor cells was found to respond to insulinlike growth factor 1 (IGF‐1) by increased osteonectin and pro‐α1 (1)‐collagen mRNA expression. Cells were treated for 24 h with insulin, growth hormone, or IGF‐1 to study the regulation of messenger RNA for osteonectin and pro‐α1(I)‐collagen using Northern blot hybridization.