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Enhancement the Enzymatic Activity of Phenol-Degrading Microbes Immobilized on Agricultural Residues during the Biodegradation of Phenol in Petrochemical Wastewater

In this study, we illustrated enhanced biodegradation enzyme activity and the strains growth using the plants residues as carriers during the biodegradation of phenol in petrochemical wastewater. The three phenol-degrading strains named as A1, A2 and A3 were selected for an immobilized microorganism technique. A1, A2 and A3 were identified as Penicilliumoxalicum, Aspergillus sp. and Sphingobacterium sp. using detailed morphological, biochemical and molecular characterization. The growth and degradation rate of phenol in wastewater by strains A1, A2 and A3 pre-grown in the agricultural residues (peanut shell) were higher than the free strains. Compared with the free strains,the enzyme activity of strains A1,A2 and A3, using the residues for pre-grown, increased 29.01 U/L, 30.30 U/L and 38.07 U/L, respectively. Hence, the immobilized microorganism technique is conducive to the phenol degradation.