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Ice Nucleation Within Peach Trees


The freezing of water within the young trees of peach [Prunus persica (L.) Batsch] was monitored using differential thermal analysis (DTA). Ice formation was initiated near −2°C. A logarithmic relationship between ice nucleation temperature and tissue sample size was observed. This suggested that the use of small tissue samples to estimate ice nucleation temperature may overestimate the extent of supercooling which would be observed in intact plants. The ice-nucleating agent associated with peach shoots active at − 2° was: 1) present in both field- and greenhouse-grown plants; 2) associated with overwintering dormant tissue and throughout blossom development; 3) resistant to surface disinfestants; 4) resistant to bacterial nucleation inhibitors; and 5) inactivated by autoclaving.