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Strawberry extract attenuates oxidative stress‐induced impaired insulin signaling in vitro in Human Skeletal Muscle Cells

Insulin resistance (IR) plays a central role in the development of metabolic syndrome and is a major cardiovascular risk factor. We recently reported that consumption of a strawberry (Str) beverage compared to placebo reduced markers of oxidative stress and inflammation and reduced the insulin requirement to achieve glucose homeostasis in response to a high fat/carbohydrate meal in overweight, relatively healthy people. Therefore, we hypothesized that polyphenolic derived from Str restore impaired oxidative stress‐mediated insulin signaling through its antioxidant properties. Studies were undertaken in human skeletal muscle cells. Oxidative stress was generated using H2O2 (50, 100 μM) for different time periods (2–12hr) along with and without Str extracts (0.1–1mg/ml). Cell viability was not effected by any of the above treatments. At the end of treatments, cells were treated with insulin (100 nM) for 10 min. Activation of Insulin Receptor Substrate (IRS‐1) using different ser/tyr phosphorylation sites were studied using immunoblotting method. Str extracts reduced the oxidative stress‐induced increased levels of inhibitory phosphorylation (ser‐307), and increased levels of tyr phosphorylation on IRS‐1. These data provide mechanistic evidence by which Str polyphenols promote postprandial insulin sensitivity and suggest a role for Str intake in reducing IR and the risk for cardiometabolic disease.