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Genetic diversity assessment of Soybean germplasm accessions using pod shattering-related SSR markers

The present investigation was carried out to characterize and validate the SSR markers linked to pod shattering trait at molecular-level in a given novel soybean germplasm during 2016-17. Efforts were made to study the DNA polymorphism via SSR markers linked to shattering in the given set of soybean accessions. The SSR markers conditioning the pod dehiscence yielded 100% polymorphism with corresponding 596 amplicons. PIC value ranged between 0.056 (Satt350) to 0.913(SRM0) with an average PIC value of 0.734 per primer. During this study, unique alleles were found for resistant genotypes (Satt244163) and highly susceptible genotypes (SRM1507), which can be directly advocative for identification of resistant and susceptible genotypes and also to generate mapping population towards fine mapping of these ‘qPDH’ QTLs in soybean functional genomics.