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Abstract 105: A Non-Suture Cuff Technique for Lymph-Vein Anastomosis in a Rat Model

Method: A total of 15 Lewis rats (age: 6–8 weeks, body weight: 252.8±3.78g) underwent lymph-vein anastomosis. The left cervical branch of the lymphatic duct that commence into the thoracic duct and the facial vein were freed and dissected. Lymph-vein anastomosis was performed in an endto-end fashion using a polyimide tube (0.368361mm as inner diameter, 0.41148mm as outer diameter), which was prepared into a wedge-like cuff. After the cuff was mounted on the lymph duct, the duct was then inverted and held in place with a circular tie. Next the facial vein was pulled over the lymph stent and anastomosis was completed with a second nylon tie over the cuff. All anastomoses were evaluated by histology on post-op day 7.