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Optical determination of intracellular water in apoptotic cells

•  Determination of intracellular water can be achieved by a combination of brightfield and transmission‐through‐dye imaging, all realized on a standard transmission microscope. •  The method permits sensitive detection of water loss in cells exposed to apoptotic agents. •  Cell water measurements are not affected by separation of apoptotic bodies and can be applied to samples where measurements of cell volume alone would be insufficient.