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Glucose measurements and clinical correlations.

Various methods for glucose analysis of whole blood and plasma are currently utilized and the clinician should be familiar with them when interpreting a glucose value. Three methods of glucose analysis of whole blood and plasma have been evaluated: Somogyi-Nelson, hexokinase, and copper reduction. Linear regression equations were derived and statistical evaluation by analysis of variance revealed highly significant results. Plasma glucose as measured by the copper reduction technique is approximately 17% higher than whole blood glucose measured by the Somogyi-Nelson method. A consistent 5-mg/deciliter difference in plasma glucose was present between the two plasma glucose procedures, the enzymatic method, and the copper reduction method.