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Novel quantitative electrophoretic analysis of endotoxins on microchips

A novel microchip electrophoresis method was developed and applied for sensitive detection and quantitative analysis of endotoxins extracted from Gram‐negative bacteria. The method provides a fast and quantitative differentiation of smooth and rough endotoxins based on the solubilization and complexation of the lipopolysaccharides with dodecylsulfate, and then with a fluorescent dye. The migration of the complexes was followed by LIF detection. The novel method is able to replace the SDS‐PAGE with the advantage of high speed and better sensitivity, and by avoiding the laborious gel‐preparation and silver staining.