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Presence of zinc and calcium permeant channels in the inner membrane of the nuclear envelope.

The nuclear envelope possesses specific ion channels that regulate the ionic traffic between the cytoplasm or the perinuclear space and the nucleoplasm. Using the patch-clamp technique to isolated rat nuclei exhibiting only the inner membrane of the nuclear envelope, we report the existence of calcium and zinc permeant channels. These channels displayed similar characteristics (conductance : 8 and 11 pS respectively, open time constant (3.5 ms and 3.7 ms) and close time constant (5.1 ms and 4.8 ms)) and were insensitive to different types of calcium channels blockers and to calcium concentration in the bathing solution. The exact role of these channels remains to define, but they may contribute to the regulation of intranuclear Ca++ or Zn++ dependent processes as important as cell proliferation or programmed cell death. Moreover, this work demonstrates that our nuclei preparation provides a way to study the inner membrane of the nuclear envelope.