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The Anti-Fatigue Effect of Polyphenols and Flavonoids Isolated from Eucommia ulmoides Leaves in Mice

The crude extract of Eucommia ulmoids leaves was separated and purified by resin, and polyphenols and flavoniods components in different contents were obtained. Two components, one of which contained more polyphenols (EUI), and one of which contained more flavoniods (EUII), were chosen for the anti-fatigue test. The anti-fatigue test shows that by comparing with negative control (NC) group, both EUI and EUII show anti-fatigue effect, however, EUI can more obviously prolong the weight-loaded swimming time, reduce the content of blood lactic acid (LA) and serum urea nitrogen (SUN), increase the content of hepatic glycogen and enhance lactate dehydrogenase (LDH) activity. In other words, the component containing more polyphenols show more anti-fatigue effect than the component containing more flavoniods. That means polyphenols might be the main ingredient in Eucommia ulmoids leaves to exhibit anti-fatigue effect.