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Comparison of the biological characteristics of primary rat macrophages extracted by three methods

BACKGROUND: Methods for extracting primary macrophages from rats include bronchoalveolar lavage fluid (BALF) extraction, spleen extraction, and peripheral blood extraction. These methods have their own advantages and disadvantages, and the cells show different activities, status and quantities. OBJECTIVE: To analyze the better selection of primary extraction and culture of macrophages from three different tissue sources. METHODS: Under the same experimental conditions, primary rat macrophages were cultured using three different methods: BALF extraction, spleen extraction and peripheral blood extraction. RESULTS AND CONCLUSION: The average numbers of primary macrophage extracted from BALF, spleen and peripheral blood were (1.14±0.14)×106, (9.29±0.19)×106 and (3.17±0.13)×106 respectively, there was a significant difference among groups (P < 0.001). The average survival time of the three methods for cell extraction was (111.00±5.98), (100.00±4.60) and (97.00±3.43) hours respectively, the difference was significant (P < 0.001). The activity of cells extracted from BALF was significantly higher than that of the other two tissues (P < 0.05), but there was no significant difference between spleen and peripheral blood (P > 0.05). These results indicate that BALF extracts the most activated macrophage can be extracted in BALF, and the largest number of macrophages can be extracted from the spleen.