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Transferability of apple and pear SSRs to other temperate pome fruit crops of family Rosaceae

Extensive use of simple sequence repeat (SSR) is facilitated if loci would be
 transferable across species even in closely related genera to overcome high
 cost and efforts involved in their development as major constraints. In the
 present study, apple and pear genomic microsatellite primer pairs were used
 to amplify SSR loci in apple, pear, quince and loquat genotypes,
 respectively. Already reported SSRs were selected based on their polymorphic
 survey for successful amplification with at least one polymerase chain
 reaction (PCR) product of the approximate size expected for a homologous
 locus screened among apple and pear genotypes for further transferability
 exploration across other temperate pome fruit crops, respectively. Highest
 transferability of apple and pear SSR, 61.53 % and 73.33 % was observed in
 closely related quince and apple genotypes, respectively. This indicated
 that primer binding sites between these two closely related genera, Malus
 and Pyrus, are fairly well conserved. Maximum transferability rate was found
 to be 93.33 % and 80.00 % across all the subjected genotypes for primer
 CH05D11 and TSUenh016 in apple and pear, respectively. The transferability
 of markers is based on genomic similarity, and can reflect the relationship
 of genome collinearity and even evolution between species. This high level
 of transferability of apple and pear SSRs to other temperate pome fruit
 crops indicated their promise for application to future molecular screening,
 map construction, and comparative genomic studies, etc.