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Androgen receptor levels in preputial skin from boys with hypospadias

The level of high-affinity low capacity binding of [3H] methyltrienolone ([3H]MT) to human preputial skin cytosol in 7 boys with hypospadias and in 7 control subjects have been measured.[3H]MT has been shown to have a specific affinity to the androgen receptor. The binding component was heat-labile, was totally destroyed by trypsin and the degradation at 0°C was slow (t 1/2=70 h). The time of dissociation of the 3H MT-receptor complex at 0°C was slow (t 1/2=55 h). DHT and testosterone displaced [3H] MT from the receptor whereas androstendione, progesterone, estradiol and R 5020 were much less effective competitors and cortisol did not compete. The dissociation constant of the 3H MT-receptor complex varied between 0.17-2.17×10-10M and the number of binding sites between 1.08-7.35 fmoles/mg protein. No significant differences were found between boys with hypospadias and control subjects. Procedure of collecting skin samples was approved by the Ethical Committee of Karolinska Sjukhuset.