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Differential analysis of allosteric behavior.

Differential analysis of allosteric systems focuses on the ratio of consecutive binding constants obtained for multiple binding as originally used by Adair. If this ratio of experimental constants is smaller than the ratio of statistical constants for multiple binding, cooperativity among subunits is indicated. The conditions are evaluated with the aid of the model of Monod et al and compared with available data on calmodulin. Calmodulin shows cooperativity among some subunits, not among others, depending strongly upon experimental details. Values of Ln (the interconversion constant of fully occupied sites) can be estimated for c much less than 1 (and Ln-1 much greater than 1).