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Studies on isobutyric acid‐producing bacteira in silage

Simulated silages were made and inoculated with an isobutyric acid‐producing bacterium to investigate its possible role in the appearance of the acid in silage. Lactobacillus brevis strain L100 proved capable of growing in maize silage and forming a large amount of isobutyric acid. In laboratory medium strain L100 did not produce the acid in either static or shaken cultures. In maize silage extract L100 Produced a high quantity of isobutyric acid only during anaerobic growth