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A demonstration that the ratios between human pancreatic enzymes can change in 4 days.

Electrophoretic studies of inactive ductal pancreatic secretions, collected on five successive days following operation, demonstrated up to 18 protein bands. Comparison of the densitometric scans on the first and fourth days was as follows: the patterns from different individuals appeared similar during the periods of slow secretion immediately following surgery, there were obvious changes in the proportions of the slower-moving enzymes between the first and fourth days; the most apparent alteration was in the trypsinogen 2 band which was markedly higher on the fourth as opposed to the first day, and during the 4th-6th days, the patterns for a given individual resembled one another, showing only minor changes among the 8-hour collections.