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Software-Based Imaging and Segmentation of Corneal Nerve Fibres

A method for imaging of expanded areas of the sub-basal nerve plexus and automatic segmentation of corneal sub-basal nerve fibres is presented. Large mosaic images are generated from image stacks and submitted to a series of image processing steps. Subsequent segmentation and reconstruction of interrupted fibre traces identifies subbasal nerve fibres reliably. Nerve fibre networks in the resulting images can be comprehensively evaluated based on morphological and topological parameters.