{'fraction_non_alphanumeric': 0.03607843137254902, 'fraction_numerical': 0.03058823529411765, 'mean_word_length': 4.907407407407407, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Nucleotide sequence of a fission yeast gene encoding the DEAH-box RNA helicase.

We have isolated a new putative ATP-dependent RNA helicase gene from fission yeast Schizosaccharomyces pombe which belongs to the DEAH-box family, and determined the entire nucleotide sequence of the gene including its 5' and 3' noncoding regions (4185 bp). Northern blot analysis has shown that mRNA for the gene is approximately 2400 nucleotides in length (Figure 1). PCR-based cDNA analyses have demonstrated the presence of two short introns (83 and 43 nucleotides) in the coding region. In each of these two introns, a putative branch point sequence (CTAAT) is located just upstream of the 3' splice site. The length of mRNA obtained from Northern blot analysis is sufficient to include the predicted coding region (2241 nt). The protein product (747 amino acid residues) deduced from the nucleotide sequence has several motifs which are characteristic of ATP-dependent RNA helicases and shows extensive similarity with DEAH-box RNA helicases' (PRP2, 16 and 22) of Saccharomyces cerevisiae which are involved in pre-mRNA splicing (1-5). Therefore, the gene which we isolated has been designated PRH 1 (pombe RNA helicase 1). The central domain of PRH 1 product (residues 119-424), which is