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Development of the model for the optical multiwavelength monitoring of creatinine in the spent dialysate

The aim of the study was to develop a statistical model suitable for the estimation of creatinine concentration removed during dialysis through UV-absorbance. Ten uremic patients, three females and seven males, mean age 62.6 ± 18.6 years, on chronic thrice-weekly hemodialysis were included in the study. Double-beam spectrophotometer (Shimatsu UV-2401 PC, Japan) was used for the determination of UV-absorbance in the collected spent dialysate samples. Due to differences in independent variables included two multiwavelength models were developed using stepwise regression. The coefficient of determination, R2 was 0.8709 for the first and 0.8756 for the second model. The standard errors were 15.86 micromol/l and 16.01 micromol/l, respectively. In summary, the creatinine concentration removed during dialysis can be estimated with UV-absorbance technique.