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[The structure of 2 promoters and a transcription terminator from the region of early genes D10-D15 of bacteriophage T5].

The primary structures of two promoters (T5 P31A and T5 P31B) and transcription terminator from the region of bacteriophage T5 early genes D10-D15 were determined. Both promoters (62% of the phage T5 DNA physical map) are located as a tandem at 70 bp distance from each other and are typical for the promoter-consensus structure of DNA of bacteriophage T5 early promoters. The transcription terminator is located in the region of the BamHI site (67% of the phage T5 DNA physical map) and is arranged in good coincidence with the consensus structure of the already known rho-independent terminators of various organisms. The promotor T5 P31A and the terminator were tested for the level of galactokinase synthesis using galK gene-carrying plasmids and D-14C-galactose as a substrate for galactokinase.