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[Action characteristics of the cell cycle nonspecific agents in cell lines in the state of continuous transartery chemotherapy infusion].

OBJECTIVE
To detect the acting pattern of different high concentrations of cell cycle nonspecific agents (CCNSA), epirubicin, oxaliplatin, cisplatin and fotemustine, acting with HepG-2 and 7702 cell lines respectively for different times; and to explore whether there are concentration and time dependent phenomena for each agent and relations between these two factors.


METHODS
MTT assays were used to detect inhibition rates of different combinations of concentrations and times in all the agents. Five concentrations were chosen for each agent within the range of clinical practice in interventional therapy by the ratios of 20:10:5:2.5:1, the highest concentrations of epirubicin, oxaliplatin, cisplatin and fotemustine was 8 mg/L, 20 mg/L, 20 mg/L and 8 mg/L respectively, and each concentration acted with HepG-2 and 7702 cell lines at 5 different time points which lasted for 1 h, 2 h, 4 h, 6 h, and 8 h, respectively.


RESULTS
Different combinations of concentrations and time points had different inhibition rates for HepG-2 and 7702 cell lines. For epirubicin and oxaliplatin, the inhibition rates of 5 concentrations had little difference, which mainly depended on time and increased with the increase of lasting time; cisplatin showed different characteristics: at low concentrations, the inhibition rate increased slowly with the increase of lasting time, but with the increase of concentration, the inhibition rate increased obviously with the increase of lasting time. The inhibition rate of fotemustine lacked obvious connection with the concentrations, but increased with the time at any cencentration.


CONCLUSION
Different CCNSA have their own suitable concentration and time factors, both factors have obvious influence on the inhibition rate, and the time factor seems more important. Since transartery infusion can provide enough or even over-dose concentrations, the minimum effective concentration and enough time should be chosen, which could increase the killing of tumor cells, and at the same time decrease the total dose, thus realizing precise artery chemotherapy.