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Early axon and dendritic outgrowth of spinal accessory motor neurons studied with dii in fixed tissues

We have utilized lateral diffusion of DiI in fixed tissues (Godement et al., '87: Development 101: 697–713) to study early axon and dendritic outgrowth of spinal accessory motor neurons in embryonic rats. Crystals were placed in the central canal of the cervical spinal cord near the ventral commissure in order to label growing accessory axons anterogradely and on the spinal accessory nerve to label somata and dendrites retrogradely. Animals were studied on E11–E13.