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[Determination of fentanyl concentrations in the blood with the application of high performance liquid chromatography].

We developed a method to determine the blood concentration of fentanyl by using reverse phase partition liquid chromatography. The extract rate of fentanyl from blood 10 ml was 79 +/- 2%, and the minimum detective concentration of fentanyl was 158 pg.ml-1. These performances are sufficient to determine the blood concentration of fentanyl in clinical anesthesia.