{'fraction_non_alphanumeric': 0.033363390441839495, 'fraction_numerical': 0.01881574992485723, 'mean_word_length': 5.1342182890855455, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 3, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
European and Asian contribution to the genetic diversity of mainland South American chickens

Chickens (Gallus gallus domesticus) from the Americas have long been recognized as descendants of European chickens, transported by early Europeans since the fifteenth century. However, in recent years, a possible pre-Columbian introduction of chickens to South America by Polynesian seafarers has also been suggested. Here, we characterize the mitochondrial control region genetic diversity of modern chicken populations from South America and compare this to a worldwide dataset in order to investigate the potential maternal genetic origin of modern-day chicken populations in South America. The genetic analysis of newly generated chicken mitochondrial control region sequences from South America showed that the majority of chickens from the continent belong to mitochondrial haplogroup E. The rest belongs to haplogroups A, B and C, albeit at very low levels. Haplogroup D, a ubiquitous mitochondrial lineage in Island Southeast Asia and on Pacific Islands is not observed in continental South America. Modern-day mainland South American chickens are, therefore, closely allied with European and Asian chickens. Furthermore, we find high levels of genetic contributions from South Asian chickens to those in Europe and South America. Our findings demonstrate that modern-day genetic diversity of mainland South American chickens appear to have clear European and Asian contributions, and less so from Island Southeast Asia and the Pacific Islands. Furthermore, there is also some indication that South Asia has more genetic contribution to European chickens than any other Asian chicken populations.

Radiocarbon dates have also been used to suggest a pre-Columbian introduction of chickens to South America [18], though there is also debate over the reliability of these radiocarbon dates [33]. The presence of mtDNA haplogroup D in an early post-European Peruvian specimen has also been used to suggest chickens from this country may have originated from Polynesia during pre-Colombian times [19]. Thus, the Americas may have experienced at least two translocations of chickens, initially by the Polynesians and subsequently by Europeans [19]. Haplotype E1 is ubiquitous worldwide and considered phylogeographically uninformative [33] and its presence in ancient Polynesian samples is suggested to be a result of laboratory contamination [20]. These issues have been discussed extensively in the literature [16,38,39].
A recent study comparing contemporary chickens from South America and the Iberian Peninsula (Spain and Portugal) suggests that the observed genetic differentiation between the two regions is due to another (unsourced Asian) maternal source for South American chickens [40]. That study indicates that despite the global movement of chicken during modern times, the genetic patterns from the initial translocation can still be inferred.
In this study, we extend both the South American and comparative sampling of the previous study to characterize the contemporary mtDNA control region (CR) DNA data from South America and compare to other chicken populations from across the globe (from Europe to Island and Mainland Southeast Asia, East Asia, the Pacific Islands, South Asia and Southwest Asia). We assess the ancestry of modern South American chickens as a potential way to infer the colonization history of the continent by Europeans and later trade networks with Asia.

Chicken samples, polymerase chain reaction and sequencing
Blood samples were collected from a total of 229 native chickens from four South American countries (excluding Easter Island, which although it is a special territory of Chile, is considered to be culturally aligned with the Pacific region): 30 from Brazil, 60 from Chile, 129 from Colombia and 10 from Peru. Blood samples were collected from the brachial vein of the wing and transferred to FTA cards (Qiagen, Hilden Germany). DNA was extracted using QIAamp DNA Investigator Kit (Qiagen, Hilden, Germany). The mtDNA CR was chosen as the target as it is highly polymorphic and phylogeographically informative [3,14,20,33,41]. The target region of mitochondrial hypervariable region 1 was amplified using the following primer set: 5 0 -AGGACTACGGCTTGAAAAGC-3 0 and 5 0 -ATGTGCCTGACCGAGGAACCAG-3 0 . DNA was amplified using polymerase chain reaction (PCR) in 30 µl reaction volumes containing 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 0.1% Triton X-100, 1.5 mM MgCl 2 , 0.2 mM dNTPs, 0.1 µM concentrations of each primer, 1.25 units of Taq DNA polymerase (Promega) and 100-200 ng of template DNA. PCR cycling condition included an initial denaturation of 94°C for 2 min, followed by 35 cycles of 25 s at 94°C, 35 s at 58°C, and 1 min 10 s at 72°C, and a final extension for 10 min at 72°C. Sanger sequencing was conducted at the Australian Genome Research Facility Ltd (AGRF) in Brisbane. The raw forward and reverse chromatograms were assembled, edited and inspected by eye to give a consensus sequence of a 530 bp fragment for each sample excluding primer sequences.

Sequence data, phylogenetic and population genetic analyses
In addition to the 229 control region sequences generated in this study, we included 2618 worldwide mtDNA control region sequences from GenBank to examine the relationship of mainland South American chickens with those from South Asia (India, Bangladesh), Mainland Southeast Asia (MSEA: Laos, Myanmar, Thailand, Vietnam), Island Southeast Asia (ISEA: Indonesia, Philippines), Pacific Islands (Fiji, Solomon Islands, Vanuatu, Easter Island), Central Asia (Azerbaijan, Turkmenistan), East Asia (China, Korea) and Europe (electronic supplementary material, table SI 1). A total of 2827 sequences were aligned using the MUSCLE [42] algorithm in Geneious v. 11.0.4 [43] and trimmed to produce a final 412 bp fragment corresponding to mtDNA CR positions 93-504 of the reference sequence NC_007235 [44]. Truncation of the new sequences to the 412 bp fragment was made to directly compare with the South American chicken samples from Luzuriaga-Neira et al. [40]. The number and assignment of haplotypes of the 412 bp CR dataset was determined using DnaSP v. 6 [45]. The haplogroup of the newly generated sequences were established by comparison with royalsocietypublishing.org/journal/rsos R. Soc. open sci. 7: 191558 sequences of known haplogroup designation [3,12]. This was executed using a combination of neighbour-joining (NJ) and median-joining (MJ) [46] analyses. jModelTest [47] was used to determine the best-fit model for the CR dataset (TIM1 + G), this was performed through the CIPRES Science Gateway [48], then an NJ tree was estimated using PAUP v. 4 [49]. The phylogenetic structure of the South American chicken sequences used in this study was also characterized by using the network analysis (MJ) implemented in PopART v. 1.7.1 [50]. The program Haplotype Viewer (http://www. cibiv.at/~greg/haploviewer) was also used to visualize the haplotype genealogies for the whole dataset.
The population genetic structure among the sampling locations was estimated using Slatkin's linearized F ST as implemented in Arlequin v. 3.5.2.2 (10 000 permutations) [51]. To visualize the relationships of the sampling populations, the F ST scores were ordered into principal coordinate analysis (PCoA) plots using GenAlEx v. 6.503 [52]. This analysis was initially performed for all populations included in the study. By removing the outliers in PC1 of this PCoA plot (responsible for approx. 30% of variation explained by PC1), we generated a second PCoA plot to investigate which geographical regions the South American chickens are most allied with. Population genetic structure was further investigated using analysis of molecular variance (AMOVA) as implemented by Arlequin v. 3.5.2.2 [51]. The groupings used in the AMOVA compared chicken populations from seven regions including South America, ISEA-Pacific, MSEA, South Asia, Europe and Central Asia. The different population hypotheses were tested initially using the overall dataset assuming no groups and hierarchically comparing populations from different geographical regions indicated previously. Significance testing was done using 10 000 coalescent simulations in Arlequin v. 3.5.2.2 [51]. Intrapopulation genetic variability statistics (i.e. segregating sites, number of haplotypes, haplotype and nucleotide diversities) were also calculated using DnaSP v. 6 [45].

Results and discussion
A previous mtDNA study revealed nine divergent haplogroups (A-I) of chickens from across the world [3]. A more fine-grained mtDNA genome phylogeny study revealed an additional four haplogroups (W-Z) [12]. Haplogroup A and B are predominantly found among southern and eastern Chinese and Japanese chickens as well as wild RJF. Haplogroup C is found mainly in Japanese and southeast Chinese chickens. Haplogroup D is found in Japanese, southeast Chinese, Mainland Southeast Asian and Pacific chickens. Haplogroup E is widespread among Indian, Middle Eastern and European chickens.
Five of these haplogroups (A-E) are relevant to the present study as they are found in South American chickens (figure 1). Haplogroup D has not been found in modern South American chickens in this study. Haplogroup E is the most predominant mitochondrial CR region lineage observed in South America comprising 83.96% of all chickens on the continent (table 1). Haplogroups A (7.35%), B (4.68%) and C (4.00%) are also observed in modern South American chickens, albeit at very low frequencies. All four of these haplogroups are related to those found in Asian and European chicken populations.
Haplogroup E is also the most dominant haplogroup observed across each of the South American countries we studied (i.e. Bolivia, Brazil, Chile, Ecuador and Peru). Given the high frequency of this haplogroup in Europe along with the historical records and observations that Spanish and Portuguese brought chickens to the Americas [36], Europe may be the more likely source of modern chickens in South America. The most ubiquitous haplogroup E lineage in South America is haplotype 107 (haplotype E1; figure 2). It is observed in all the South American populations in this study. This haplotype potentially represents the founding haplotype brought by the Europeans. Furthermore, haplotype 107 is the lineage observed in archaeological chickens in Europe [10].
The presence of haplogroups A, B and C in South American chickens could represent subsequent introductions from Asian into the South American populations. Haplogroup D profiles are not observed in modern South American chickens from the mainland. Rather, haplogroup D is the dominant haplogroup in the Pacific Island [20] and Island Southeast Asian [14] chickens. Thus, this potentially indicates that the translocation history of haplogroup D chickens from Island Southeast Asia into the Pacific islands did not include the successful introduction of Polynesian chickens into continental South America.
The PCoA analyses (figures 3 and 4) shows that the genetic relationship of South American chickens is largely allied with European populations in comparison to other parts of the world. In particular, this affinity is more pronounced with chickens from the Iberian Peninsula (i.e. Portugal and Spain). The royalsocietypublishing.org/journal/rsos R. Soc. open sci. 7: 191558 geographical group of chickens with the next closest affinity to those in South America are South Asian chickens, then East Asian and Southeast Asia. It can also be observed that South American chickens are only remotely related to chickens from the Pacific, despite their geographical proximity (figure 3). In general, the modern diversity of South American chickens does not appear to support a pre-Colombian Polynesian origin. If a pre-Columbian introduction of chickens to South America did happen, its genetic signature did not persist through to modern times. Furthermore, the close relationship of European chickens to those from South Asia seems to potentially suggest a history based on initial domestication in that region and subsequent translocation(s) to Europe. The archaeological documentation of chicken remains from the Indus Valley suggests that South Asia was indeed one of the domestication centres for chickens [7]. The cultural contact, trade and migration of this early civilization probably allowed for the human-mediated transport of chickens from South Asia to Western Asia, through the Arabian peninsula and then on to the Mediterranean region [7]. One of these trajectories could also have involved the movement and introduction of chickens to the east coast of Africa [14].
The PCoA plot (figure 4) also indicates that Iberian chickens are more closely related to chicken populations from the western side of the South American continent (i.e. Columbia, Chile, Ecuador) than the eastern side (i.e. Brazil). This could potentially reflect the trading network between Spain, Portugal, South America and the Orient. The initial European voyages into South America involved a long-distance trans-Atlantic crossing from the Iberian Peninsula arriving at the West Indies or at several mainland port cities such as Veracruz in Mexico and Panama-Porto Bello. Then, trade and exchange via the land caravan or China Road (Camino de China) occurred between Vera Cruz on the Atlantic side and Acapulco on the Pacific side (via Puebla) [53]. Mexico City was also part of these early over-land trade routes [54]. From Acapulco, merchant ships sailed west across the Pacific to Manila via the Manila galleon trade seeking oriental products [55], but ships could also have proceeded to other ports on the western coast of South America [56]. The maritime network from Acapulco to other ports on the west coast of South America is highly likely, based on the availability  A pre-Columbian contact between South America and Polynesia has been suggested by other lines of evidence [22,24,25]. Some researchers hypothesize that chickens were initially introduced to South America by the Polynesians before the initial arrival of Europeans [18]. This scenario appears to be tenuous when using modern DNA chicken datasets, as South American chicken populations are clearly more related to chickens from Europe. However, this does not discount the potential for a pre-Columbian introduction of chickens by the Polynesians to South America. All we can say is that there is no evidence for Polynesian chicken genetic signatures being retained by contemporary South American chickens. Caution has to be taken in reconstructing certain aspects of past human behaviour when using only modern DNA, especially of commensal animals [16,19,57]. It remains possible that these Polynesian chicken introductions into South America were not in high enough numbers to survive and have a genetic impact on modern-day chicken populations on the continent.
Additionally, it appears that contemporary European chickens are more allied with South Asian chickens than any other continental or insular East Asian chicken populations (table 2, AMOVA). This may suggest a more southern route to Europe from South Asia through Persia and Greece [7] rather than the northern alternative through China and Russia [4], although without any Russian samples, this is speculation only. Furthermore, the posited natural range (South Asia, Southeast Asia) of chickens retains a high level of genetic diversity (table 3).

Conclusion
The present-day global landscape of chicken genetics still appears to reflect, to some degree, the processes that allowed them to spread to regions outside the biogeographic range of their ancestors. While we can speculate that some of the original colonization patterns have been overwritten by the global commercial transport of chicken during modern times, the extent of this overwriting is uncertain. In the current study, we illustrate that modern genetic diversity of South American chickens reflects that from their Table 2. Population genetic structure estimated from the analysis of molecular variance (AMOVA) based on the chicken mtDNA D-loop sequences from (1) South America, (2) ISEA-Pacific, (3) East Asia, (4) MSEA, (5) South Asia, (6) Europe and (7)   Headquarters considers that the activities proposed to carry out this research do not involve procedures against animal welfare. Therefore, the study is classified as without risk.
Data accessibility. All mtDNA control region sequences generated by this study are available in GenBank under accession numbers (MN149634-MN149862).