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Probing drug–protein interactions with MicroED

Microcrystal electron diffraction (MicroED) permits structure determination from nano/microcrystals using a cryoelectron microscope, bypassing the need for large crystals that are especially challenging to obtain for many samples. Furthermore, microcrystals improve the efficiency of ligand soaking, allowing for rapid structure determination of multiple drug-protein complexes using the same crystalline sample. As such, MicroED is a valuable technique for drug discovery. Here, we summarize the process of protein-ligand structure determination by MicroED. We also present several structures solved by our lab, which showcase the versatility of MicroED and its potential for structure-based drug design targeting both soluble and membrane proteins.