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Sulfated Hyaluronan Derivatives Modulate TGF-β1:Receptor Complex Formation: Possible Consequences for TGF-β1 Signaling

Glycosaminoglycans are known to bind biological mediators thereby modulating their biological activity. Sulfated hyaluronans (sHA) were reported to strongly interact with transforming growth factor (TGF)-β1 leading to impaired bioactivity in fibroblasts. The underlying mechanism is not fully elucidated yet. Examining the interaction of all components of the TGF-β1:receptor complex with sHA by surface plasmon resonance, we could show that highly sulfated HA (sHA3) blocks binding of TGF-β1 to its TGF-β receptor-I (TβR-I) and -II (TβR-II). However, sequential addition of sHA3 to the TβR-II/TGF-β1 complex led to a significantly stronger recruitment of TβR-I compared to a complex lacking sHA3, indicating that the order of binding events is very important. Molecular modeling suggested a possible molecular mechanism in which sHA3 could potentially favor the association of TβR-I when added sequentially. For the first time bioactivity of TGF-β1 in conjunction with sHA was investigated at the receptor level. TβR-I and, furthermore, Smad2 phosphorylation were decreased in the presence of sHA3 indicating the formation of an inactive signaling complex. The results contribute to an improved understanding of the interference of sHA3 with TGF-β1:receptor complex formation and will help to further improve the design of functional biomaterials that interfere with TGF-β1-driven skin fibrosis.

extracellular matrix (ECM) and plays a critical role in all phases of wound healing as it has a distinct influence on the regulation of multiple cellular responses. It is found at high levels in the wound microenvironment, where it promotes fibroblast chemotaxis, myofibroblast differentiation and induces fibroblasts to synthesize and contract extracellular matrix for wound contraction 1,2 . Consistent overexpression of TGF-β1 during wound healing leads to an excessive accumulation of ECM proteins which clinically manifests in fibrotic skin disorders, like hypertrophic scarring, keloids and localized or systemic sclerosis [18][19][20] . TGF-β1 is known to interact with heparin and heparan sulfate (HS). Binding to these glycosaminoglycans (GAGs) potentiates TGF-β1 activity and prevents proteolytic degradation in vitro 21,22 . Hintze et al. demonstrated specific interactions between chemically sulfated hyaluronan HA (sHA) derivatives and TGF-β1 where the respective binding strength depended on the degree of HA sulfation and the highly sulfated HA (sHA3, three sulfate groups per repeating disaccharide unit of HA) exhibited the strongest interaction with TGF-β1 23 . In previous studies sHA derivatives have been shown to be a promising tool for investigating the structure-function relationship of GAGs in their interaction with biological mediators [23][24][25][26][27][28] . Compared to natively sulfated GAGs such as heparin they have defined properties regarding their monosaccharide composition and sulfation. Van der Smissen et al. investigated the consequences of TGF-β1 interaction with sHA derivatives on its bioactivity in vitro 29 . They revealed an impaired Smad2/3 translocation to the nucleus in the presence of sHA3 in human dermal fibroblasts and proposed that sHA prevents interaction of TGF-β1 with TβR-I and -II. In the present study, the impact of sHA on the TGF-β1:receptor complex formation was investigated to explain the reduced TGF-β1 bioactivity. Using surface plasmon resonance (SPR) combined with molecular docking and molecular dynamics simulation techniques the complex interaction of TGF-β1 and both TGF-β receptors with sHA derivatives were examined for the first time. Moreover, the consequences of TGF-β1/GAG interaction and altered TGF-β1:receptor complex formation for TGF-β1-mediated TβR-I phosphorylation was examined using Western Blot analysis. In addition, TGF-β1-induced phosphorylation of Smad2 and Erk1/2 was evaluated.

Results
Characterization of Polymeric HA Derivatives. Low-, medium-and high-sulfated HA derivatives (sHA1, D.S. = 1.0; sHA2, D.S. = 1.8; sHA3, D.S. = 2.8) with reduced molecular weight (MW (LLS) of 26 kDa, 29 kDa and 21 kDa, respectively) were synthesized and characterized as described previously 24,29 . As a non-sulfated reference material, a low molecular weight HA with a molecular weight of M W (LLS) of about 48 kDa was prepared by controlled thermal degradation of native high molecular weight HA. 13 C-NMR investigations of this degraded HA did not show any structural changes compared to native HA.
Characterization of Tetrameric HA Derivatives. The persulfated and anomerically fixed HA degree of polymerization (dp) 4 was characterized by proton and carbon NMR-spectroscopy. The signals of the sugar ring showed the expected downfield shifts in the range of e.g. 0.46-0.61 ppm for the anomeric protons compared to the non-sulfated azide of HA dp4. The mass spectrometric analysis required a counter-ion exchange of all sulfate residues from sodium to tetraethylammonium (TEA) to reduce sulfate loss during the ionization process. The obtained ESI-MS-spectra of the per-sulfated azide of HA dp4 showed multiple charged species and different numbers of attached TEA counter-ions as described in Köhling et al. 30 . The average sulfate content of 80% of all mass signals that were detected and assigned amounted to a value of 8.8 sulfate groups per ion.

SPR Analysis of TGF-β1 Binding to TβR-I and TβR-II after Pre-incubation with GAG
Derivatives. The impact of TGF-β1/GAG interaction on the TGF-β1:receptor complex formation was analyzed via SPR. Single TβRs were immobilized onto sensor chip surfaces and their interaction with TGF-β1 pre-incubated with different concentrations of HA derivatives was evaluated. While GAG derivatives alone did not bind to TβRs, increasing concentration and sulfation of the sHA derivatives led to a significantly decreased binding of TGF-β1 to TβR-II (Fig. 1B) and TβR-I (Fig. 1C). sHA3 had the most pronounced impact of all sHA derivatives studied. The binding response of TGF-β1 to TβR-II decreased with increasing concentrations of polymeric sHA3 but only up to a concentration of 20 µM related to the molecular weight of disaccharide units (D.U.). At concentrations above 20 µM D.U. the binding response increased again and ultimately reached a plateau at concentrations above 200 µM D.U. (Fig. 1D). At concentrations above 20 µM D.U. a change in the binding curves for TGF-β1 binding to TβR-II was detected, where the typical shape changed to a linear and monotonously rising one. This effect was also observed for TGF-β1 pre-incubated with sHA2 but not for sHA1 and HA (Supplementary Figure S1). In contrast to the findings for TβR-II, binding of TGF-β1 to TβR-I was completely suppressed in the presence of 20 µM D.U. sHA3 (Fig. 1E). To determine the minimal binding sequence required to interfere with the interaction of TGF-β1 and its receptors HA dp4 and persulfated HA (psHA) dp4 were used. psHA dp4 inhibited binding of TGF-β1 to the TβRs as well (Fig. 2). As for the polymeric HA derivatives the effect on TGF-β1 binding to TβR-I was more pronounced compared to TβR-II. In contrast, HA dp4 only slightly inhibited the binding of TGF-β1 to its receptors and did not exhibit a concentration-dependent effect.
Sequential SPR Analysis of TGF-β1:Receptor Complex Formation in the Presence of Different GAG Derivatives. The impact of HA derivatives on the sequential formation of the TGF-β1:receptor complex containing both receptors was investigated. TβR-II was immobilized on a sensor chip surface and the other components were injected sequentially in the following order: 40 nM TGF-β1, 100 µM D.U. GAG and 40 nM TβR-I. The recruitment of TβR-I to the complex of TβR-II/TGF-β1/sHA3 was significantly enhanced, compared to complexes with no GAG derivative (TGF-β1, buffer, TβR-I, Fig. 3B and D). A similar effect occured for psHA dp4, when injected in the same sequential order ( Fig. 3C and E). When pre-formed TGF-β1/sHA3 complexes where injected first followed by the injection of TβR-I, recruitment of TβR-I to the complex was enhanced as well, although the binding of TGF-β1/sHA3 pre-formed complexes to TβR-II was lower compared to TGF-β1 alone (Fig. 3B). For both polymeric HA and HA dp4 there was no enhanced TβR-I recruitment.
Molecular Modeling of the TGF-β1/TβR-I/TβR-II/GAG System. Docking calculations and MD simulations were carried out to investigate the molecular mechanism of enhanced TβR-I recruitment to the complex of TβR-II/TGF-β1/sHA3 found in SPR measurements. When docking GAGs to TGF-β1/TβR-II, we found poorly clustered and broadly distributed docking solutions (Fig. 4A, upper panel). Some of them spatially overlapped with the TβR-I binding site and for this reason could not be used to explain the experimental results obtained for TβR-I recruitment. Binding poses corresponding to the obtained loose clusters non-overlapping with the TβR-I binding site were extracted for further analysis using an MD approach and free energy calculations. GAGs in these binding poses did not promote the association of TGF-β1/TβR-II/GAG with TβR-I. However, when docked to TGF-β1/TβR-II/TβR-I, a clear GAG binding pose could be predicted (Fig. 4A, down panel). Analyzing docking solutions obtained for the TGF-β1/TβR-II/GAG complex, we found the same binding pose within the top 50 solutions, though, in this case, it was not representative in terms of clustering. MD analysis of this binding pose showed that it energetically favors the association of TGF-β1/TβR-II/GAG with TβR-I in case of HA463′ but not in case of HA, and that the corresponding interactions are electrostatically driven ( Table 1). Analysis of the per residue impact to TGF-β1/TβR-II/TβR-I/GAG complex association showed that residue Lys40 of TβR-I (Fig. 4B) plays a key role for the interactions with a pre-bound GAG through its carboxyl and sulfate groups.

Influence of GAG Derivatives on TGF-β1-mediated TβR-I and Smad2 Phosphorylation. Previous
experiments showed that TGF-β1:receptor complex formation was altered in the presence of sHA3. Western Blot analyses were performed to determine the consequences of this change on the phosphorylation of TβR-I and the second messenger molecules Smad2 and Erk1/2. Hs27 fibroblast cells treated with 10 ng/ml TGF-β1 displayed a strong Smad2 phosphorylation signal compared to the unstimulated control. Stimulation with pre-formed TGF-β1/sHA3 complexes led to a decrease in TGF-β1-mediated Smad2 phosphorylation at all time points investigated, which was significant after 5 min ( Fig. 5A and B). TβR-I was already phosphorylated in untreated cells, but was enhanced in the presence of 10 ng/ml TGF-β1. Treatment with TGF-β1/sHA3 complexes reduced TβR-I phosphorylation compared to the total amount of TβR-I, although the differences are not significant ( Fig. 5A and C). Regarding the phosphorylation of Erk1/2 no influence of sHA3 was observed (Supplementary Figure S2).

Discussion
Chemically sulfated HA derivatives are promising candidates for engineering functional biomaterials since their sulfate groups modulate binding and bioactivity of growth factors, which in turn can influence healing processes. Previous studies by van der Smissen et al. showed sHA derivatives to impair TGF-β1 downstream signaling by reducing Smad2/3 translocation to the nucleus. In silico docking experiments suggested that occupation of the receptor binding sites on TGF-β1 by sHA might be responsible for this effect 29 . The aim of the present study was twofold: to achieve better understanding of sHAs' impact on TGF-β1:receptor complex formation by combining SPR analysis and computational approaches and to determine the consequences of an altered TGF-β1:receptor complex formation on the receptor level by investigating TβR-I phosphorylation in addition to phosphorylation of the TβR-I regulated effector protein Smad2 15 . Studies by Hintze et al. revealed that TGF-β1 interacts with sHA derivatives in a sulfation-dependent manner, demonstrating the strongest interaction with highly sulfated sHA3 23 . In the present study, pre-incubation of TGF-β1 with sHA derivatives blocked the binding of TGF-β1 in particular to TβR-I, but also to TβR-II. sHA3 exhibited the strongest inhibitory effect and completely blocked binding of TGF-β1 to TβR-I at 20 µM D.U., while binding to TβR-II was not fully constrained even at concentrations above 200 µM D.U. sHA3. This is in line with the low affinity of TβR-I for the ligand alone (K D = 70 µM), while the affinity of TβR-II is considerably higher (K D = 190 nM) 31 . In our experiments, the lower affinity of TβR-I was reflected by the fact that a three times higher concentration of TGF-β1 was needed to receive a binding response for TβR-I comparable to TβR-II. However, inhibition of binding to TβR-I might not be as relevant as to TβR-II, since interactions between TGF-β1 and TβR-I in vivo are barely detectable 31 . The SPR data are consistent with observations from previous studies including TGF-β1 and sHA derivatives predicting a sulfation-dependent occupation in particular of the TβR-I binding site of TGF-β1 by tetrameric sHA derivatives 29 . sHA preferred the TβR-I binding site on TGF-β1 due to a more favorable distribution of positively charged residues, which are important for GAG recognition. Longer GAG molecules bound to the TβR-I site might impair binding of the ligand to the TβR-II site as well, since the binding sites are in close proximity and thus sterical hindrance could occur. In binding experiments of the present study psHA dp4 exhibited a strong inhibitory effect on the association of TGF-β1 and TβR-II as well, though weaker than the effect on TGF-β1 binding to TβR-I. The interference of GAGs with TβR-I binding was dependent on GAG net charge indicating the importance of electrostatics in these interactions. Interestingly, an increase in binding response for binding of TGF-β1 to TβR-II could be observed in the presence of sHA3 at concentrations above 20 µM D.U. compared to lower sHA3 concentrations. Moreover, the curvature in the sensorgrams was different for binding of TGF-β1 to TβR-II in the absence of sHA3. The slope of the binding curves was more linear and binding to TβR-II did not further decrease. TGF-β1 and sHA3 might have formed complexes that were able to bind TβR-II even though the binding strength was weaker than for TGF-β1 alone. This is consistent with previous docking experiments revealing that GAGs only partially impair TGF-β1 binding to TβR-II 29 . As TGF-β1:receptor complex formation is described as an ordered-sequential assembly mode sequential SPR experiments were performed. In vivo, the different affinities of TβR-II and TβR-I to the ligand dictate a sequential order of complex assembly: TGF-β1 binds TβR-II first, followed by the recruitment of TβR-I 8,9 . Interestingly, a significantly stronger interaction of TβR-I with the complexes of TβR-II/TGF-β1/sHA3 and TβR-II/TGF-β1/psHA dp4 was observed, which did not occur in the presence of HA or HA dp4. In contrast to our findings with pre-formed TGF-β1/sHA complexes, binding of TβR-I to TβR-II/TGF-β1/sHA3 was not blocked by sHA3, but the interaction was enhanced compared to TβR-I binding to TβR-II/TGF-β1. Thus, when TGF-β1 and sHA3 were injected consecutively, TGF-β1 bound to TβR-II still had free binding sites for sHA3. A similar effect was observed with psHA dp4, even though the recruitment was lower compared to polymeric sHA3. When TGF-β1 and sHA3 were injected as pre-formed complexes over immobilized TβR-II in the sequential experiments TβR-I recruitment to the complex of TβR-II/TGF-β1/sHA3 was enhanced as well. This further indicates that TGF-β1/sHA3 complexes with sHA3 concentrations above 20 µM D.U. were able to bind TβR-II. In general, the binding levels for this interaction were low, due to a lower immobilization level of TβR-II. However, a stable binding of TGF-β1/sHA3 complexes to TβR-II was already shown in Fig. 1D. Recruitment of TβR-I was still enhanced, but due to the lower binding response of pre-formed TGF-β1/sHA3 complexes the effect was less pronounced compared to consecutively injected TGF-β1 and sHA3 showing a higher binding signal. Molecular modeling supported the sequential SPR findings and provide further insights into the potential molecular mechanism underlying the effect of GAG recognition on the function of TGF-β1:receptor:GAG system. There were different binding poses for GAGs depending on the presence or absence of the receptors. If the TβRs were not included in the docking experiment, GAGs interfered with the TGF-β receptor binding sites. However, inclusion of the receptors led to alternative putative binding poses for the GAGs. In the presence of TβR-II alone these were broadly distributed, but if TβR-I was included a clear binding   fibroblasts incubated with sHA3 showed a reduced translocation of the second messengers to the nucleus 29 which is in line with our data showing a reduced Smad2 phosphorylation in a fibroblast cell line. In addition, a trend for a decreased TβR-I phosphorylation in the presence of sHA3 was observed as well. In the TGF-β1 pathway, Smad2 and Smad3 are receptor-regulated effector proteins, specifically phosphorylated by activated TβR-I 15 . A reduced Smad2 phosphorylation would thereby be coherently explained by a previously reduced receptor phosphorylation due to altered TGF-β1:receptor formation. For the phosphorylation of Erk1/2, however, no impact of GAGs could be observed. This might be due to Erk1/2 being regulated by a multitude of other factors and not being activated by the Smad pathway 33,34 . Binding studies with the sulfated polysaccharide fucoidan showed an inhibitory effect on the binding of TGF-β1 to TβR-II resulting in a decreased Smad2 phosphorylation as well 35 . However, in this study a three times lower concentration of sulfated polysaccharide was used compared to the present study, and TβR-I was not included in the binding studies. In contrast to this, Lyon et al. found that heparin and highly sulfated liver HS potentiated the activity of TGF-β1 in rat kidney fibroblasts, indicating that there is no competition between GAG binding and receptor binding but some form of cooperativity. Nevertheless, the effect was only observed in the presence of α 2 -macroglobulin (α 2 M) and therefore ascribed to an antagonistic effect of heparin and HS rescuing the inactivation of TGF-β1 by α 2 M, rather than to the modulation of TGF-β1:receptor interaction 21,36 . Chemically sulfated dextrans were also found to potentiate the biological activity of TGF-β1, but this was explained by a mere protection of TGF-β1 from proteolysis upon complex formation with dextrans 37 . It should also be taken into account that conclusions are based on cell experiments with mink lung epithelial cells. We recognize that our study has potential limitations. The receptors used for SPR experiments are Fc-fusion proteins. As these receptor chimera are dimeric avidity through bivalent interactions cannot be excluded in sequential SPR experiments and TβR-II and TβR-I might form a ligand-independent complex in addition to complexes including TGF-β1. The enhanced TβR-I recruitment, however, is ligand-dependent as sHA derivatives do not bind to the receptors. Another potential limiting factor may be the fact that native TGF-β receptors are transmembrane receptors with a cytoplasmic tail involved in large functional complexes. Our study only uses the ECDs of the receptors in a simplified in vitro model, allowing only for a part of the complex interplay of the components in vivo. The present study reveals the underlying mechanism of reduced TGF-β1 bioactivity in fibroblast cells in the presence of sHA3. The findings have a strong impact on the elucidation of the mode of action of sHA derivatives, showing that they affect the association of TGF-β1 with both TGF-β receptors and that the order of binding events is highly important. By interacting with TGF-β1 GAGs subsequently alter TGF-β1:receptor complex formation either by blocking the interaction of TGF-β1 with its receptor or by partially forming a complex that does not activate the Smad signaling pathway. Together with van der Smissen et al. our data suggest that sHA derivatives are promising candidates for biomaterials as their inhibitory effect on TGF-β1 bioactivity might be useful to locally interfere with TGF-β1 driven skin fibrosis. Whether our in vitro findings can be translated in vivo needs to be evaluated extensively in future work.

Methods
Materials. Hyaluronan (from Streptococcus, MW = 1. Preparation of Polymeric HA Derivatives. Low molecular weight HA (LMW-HA) was produced via thermal degradation of native high molecular HA as described previously in Kunze et al. 38 Low-, medium-and high-sulfated HA derivatives (sHA1, sHA2, sHA3) were synthesized and analytically characterized as described in Hintze et al. 23,24 . Analytical data of the prepared HA derivatives (LMW-HA, sHA1, sHA2, sHA3) are summarized in Table 2.
Preparation of Tetrameric HA Derivatives. Non-sulfated HA tetrasaccharide (degree of polymerization (dp) 4) was produced via enzymatic digestion of native HA with bovine testes hyaluronidase. Persulfated HA dp4 was synthesized after fixation of the anomeric configuration of the reducing end as azide with 2-chloro-1  ,3-dimethyl-imidazolinium chloride and sodium azide in the presence of N-methylmorpholine as base. The hydroxyl groups of the corresponding anomeric azide were chemically sulfated using sulfur trioxide pyridine complex as sulfating agent. After purification by dialysis, HA dp4 was obtained as nona-sulfate sodium salt (psHA, dp4) as described in Köhling et al. 30 .

Immobilization of TβR-II and TβR-I on Sensor Chips. For interaction analysis of growth factor and
receptors in the presence of GAGs, a BIACORE T100 instrument (GE Healthcare) was used. TβR-II and TβR-I, respectively, were immobilized on the surface of a Series S Sensor Chip C1 at 25 °C using the amine coupling reaction as described by the manufacturer resulting in an average of 200 RU TβR-II and 130 RU TβR-I immobilized to the chip surface using a concentration of 100 µg/ml. As a reference one flow cell was activated and directly deactivated without immobilizing the receptors. HBS-EP (0.01 M HEPES (pH 7.4), 0.15 M NaCl, 3 mM EDTA, 0.05% surfactant P20) was used as running buffer. Prior to interaction analysis, the chip surface with immobilized receptors was blocked with three injections of 1% (w/v) BSA, 5% (w/v) sucrose in HBS-EP (3 × 700 s at 30 µl/min). After three start up injections with running buffer, pre-formed TGF-β1/GAG complexes or TGF-β1 alone were injected for 120 s at 30 µl/min and binding levels were recorded 10 s before injection stop. Additionally, 200 µM D.U. GAG alone were injected over the immobilized TβRs as a control. The injection was followed by a 10 min dissociation phase in running buffer at a flow rate of 30 µl/min. The sensor chip surface was regenerated after each sample injection with 20 mM HCl for 2 min at a flow rate of 5 µl/min 39 . The baseline was allowed to stabilize for 1000 s with running buffer prior to injection of the next sample. Data represent the mean of three independent measurements.

Sequential SPR Analysis of TGF-β1:Receptor Complex Formation in the Presence of Different GAG Derivatives.
TβR-II was immobilized on the sensor chip surface and three consecutive injections of TGF-β1, GAG derivatives and TβR-I were performed in each running cycle. 40 nM TGF-β1 were injected for 120 s at 30 µl/min with a dissociation phase of 30 s in running buffer. Afterwards 100 µM D.U. of the respective GAG derivative were injected under the same conditions followed by the injection of 40 nM TβR-I with a dissociation time of 300 s. Additionally, 40 nM TGF-β1 and 100 µM D.U. of the respective GAG derivative were pre-incubated for 1 h at RT. The pre-formed growth factor/GAG complexes were injected over immobilized TβR-II, followed by a buffer injection and the injection of TβR-I. The sensor chip surface was regenerated after each running cycle with a 30 µl pulse of 5 M NaCl in 30 mM NaOH followed by two injections of 20 mM HCl for 120 s at a flow rate of 5 µl/min. Data were double referenced by the response of the reference surface and the response of HBS-EP buffer alone relative to a baseline report point. Binding parameters were evaluated using the BIACORE T100 evaluation software 2.03. Molecular Docking. Autodock 3 (AD3) 40 was used for docking GAG molecules to TGF-β1/TβR-II and TGF-β1/TβR-II/TβR-I complexes. The protein and receptor coordinates were obtained from their experimental crystal structure at the Brookhaven Protein Databank (PDB ID: 3KFD, 2.99 Å). HA (-GlcU-GlcNAc-) n and HA463′ (-GlcU3S-GlcNAc4S6S-) n of length dp4 and dp8 were left completely flexible in the docking runs. An atomic grid with the 0.375 Å spacing was used. 100 independent runs of the Lamarckian genetic algorithm with an initial population size of 300 and a termination condition of 10 5 generations or 9995·10 5 energy evaluations were carried out. The 50 top docking solutions were clustered using the DBSCAN algorithm 41 .

Molecular Dynamics.
The TGF-β1/TβR-I/TβR-II complex and the complexes with GAGs obtained by docking were simulated with the AMBER 11.0 package 42 using ff99SB force field parameters for the protein molecule and GLYCAM06 for GAG molecules. These complexes were solvated with TIP3P water molecules in an octahedral periodic box with a minimal distance to the periodic box border of 6 Å and neutralized by counterions. The molecular dynamics (MD) simulations were run as described previously 43 with MD productive runs of 10 ns. MM-PBSA free energy calculations of protein-GAG binding and MM-GBSA (igb = 2) per residue energy decomposition were done for 100 frames evenly distributed in each MD trajectory.

Cell Culture and Western Blot Analysis of TβR-I Phosphorylation.
Hs27 fibroblast cells