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Oxytalan fibres in the human tympanic membrane

Intact tympanic membranes were removed complete with malleus and annulus from temporal bones taken from cadavers in the postmortem room. These membranes were then immediately fixed in 10 per cent Formol saline. By incising the periosteum and reflecting the handle of the malleus from the mucosal surface of the drum, an exact dissection of the malleus from the surface of the tympanic membrane was achieved. The specimens were fixed for forty-eight hours. The staining technique is essentially as described by Rannie (1963), in which Oxone* is used as the oxidizing agent. A brief account of the steps taken in this method as applied to whole thickness preparations will now be given. This is a modification of the method used by Simpson (1967), for the examination of periodontal membranes. After oxidation for thirty minutes in 12 per cent Oxone in H2O the specimens were washed thoroughly in tap water for a further thirty minutes. The membranes were then dehydrated in ascending alcohols up to 70 per cent prior to immersion in Orcein (Taenzer-Unna) prepared as follows: