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Genetics and Biochemistry of Pyrimidine Biosynthesis in Bacillus subtilis: Linkage Between Mutations Resulting in a Requirement for Uracil

A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipient of deoxyribonucleic acid extracted from phenotypically similar mutant strains. The results yielded data resulting in a fine-structure map in which all mutations were found to be linked in a small region corresponding to that previously published as the uracil region of the Bacillus subtilis genome.