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Quantitative phase imaging using three-wavelength optical phase unwrapping

We present quantitative phase images of cells and other microscopic samples, using three-wavelength optical phase unwrapping. Laser diodes and a ring dye laser are used in a standard four-frame phase shifting interferometer to obtain phase profiles with larger beat wavelengths, thus removing 2π ambiguities without increasing phase noise. The effectiveness of three-wavelength optical phase unwrapping with both laser diodes and a ring dye laser as light sources is demonstrated.