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Cross-type optical particle separation in a microchannel.

A continuous, real-time optical particle separation, which was previously delineated theoretically, is successfully implemented experimentally for the first time. In this method, particles suspended in a flowing fluid are irradiated with a laser beam propagating in a direction perpendicular to direction of fluid flow. Upstream of the laser beam, the particles move parallel to the direction of fluid flow. When the particles pass through the laser beam, the scattering force pushes them in the direction of laser beam propagation, causing the particles to be displaced perpendicular to the fluid flow direction. This displacement, known as the retention distance, depends on the particle size and the laser beam parameters. Finally, the particles escape from the laser beam and maintain their retention distances as they move downstream. In the present work, the trajectories and retention distances of polystyrene latex microspheres with three distinct diameters were monitored and measured using cross-type optical particle separation. The measured retention distances for different-sized particles were in good agreement with theoretical predictions.