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Provitamin D photoisomerization as possible UV-B monitor: kinetic study using tunable dye-laser

An in vitro model of the well-known reaction of previtamin D photosynthesis in presence of UVB (280 to 315 nm) irradiation is proposed for the measuring of solar (and artificial) UVB radiation. The detailed study of the wavelength effect on the reaction kinetics using laser irradiation, as well as computer simulations, confirms the potential of the photoreaction as spectrally selective monitor of solar UVB. A method of express spectral analysis of the photoisomer mixture has been developed to promote realization of the proposal.