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Selective plane illumination microscopy with a light sheet of uniform thickness formed by an electrically tunable lens

Light sheet microscopy is a powerful technique for rapid, three‐dimensional fluorescence imaging of large specimen such as drosophila and zebrafish embryos. Yet, beam divergence results in a loss of axial resolution at the periphery of the light sheet. Here, we demonstrate how an electrically tunable lens can be utilized to maintain the minimal, diffraction‐limited thickness of the light sheet over a wide field of view (>600 µm) at high frame rates (40 fps). This mode of operation is necessary for the application of fluorescence fluctuation spectroscopy in images. Microsc. Res. Tech. 81:924–928, 2018. © 2016 Wiley Periodicals, Inc.