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DNA Detection of Low-intensity Schistosoma japonicum Infection by Nested-PCR Assay in Serum of Host

Objective To study the DNA detection of low-intensity Schistosoma japonicum infection in serum of host for early diagnosis and therapy evaluation of Schistosomiasis. Methods A nested-PCR assay was established and optimized to detect the S. japonicum specific DNA from the sera of rabbits during 3d to 19w post-infection. Results After infection,a S. japonicum specific DNA fragments were obtained from all the sera samples of rabbits in which 3 ～ 5 pairs of worms lived,especially,that were detected at the third day after infection. Conclusion The nested-PCR is a potential tool in diagnosis of low-intensity S. japonicum infection.