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The application of 13C-NMR spectroscopy to study lipid A from Yersinia pseudotuberculosis lipopolysaccharide.

The use of 13C-NMR spectroscopy in the establishment of lipid A backbone structure from lipopolysaccharide of Yersinia pseudotuberculosis has been described. The 13C-NMR spectra of degraded lipid A and its N-acetate were obtained. The assignment of signals was made by comparison with the chemical shifts for 13C-NMR spectra of glucosaminitol, N-acetyl-glucosaminitol and their beta-1,4 and beta-1,6 disaccharides. It was shown that lipid A backbone of the lipopolysaccharide in question consists of beta-1,6-linked glucosamine disaccharide.