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Regulation of Mitotic Microtubule Dynamic Instability in Monopolar Spindles by Bundling and Kinetochore Attachment

The dynamics of mitotic microtubules are important for mitotic spindle assembly and chromosome segregation, but descriptive parameters have proved challenging to measure directly. We are studying the dynamic instability of single and bundled mitotic microtubules (MTs) that originate from the spindle pole bodies in fission yeasts. We marked the α‐tubulin with an m‐Cherry tag expressed at low levels to minimally perturb MT dynamics and labeled the kinetochores with a GFP tag. Using a temperature sensitive kinesin‐5 allele (cut7–24) at restrictive temperature (37°C) in which bipolar spindle assembly is blocked, we observe monopolar spindles whose dynamic MTs can be imaged directly.