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Molecular cloning and expression analysis of betaine transporter gene in maize (Zea mays L.)

During exposure to stress, betaine content was reported to increase in maize (Zea mays L.), but the exact mechanism of this increase is not clearly understood. In the current study, we attempted to identify a novel betaine transporter gene and investigated the expression patterns under salt and drought stresses. The betaine transporter gene, ZmBetProt, was cloned by RACE technique from Maize, which covered 1538 bp with a 1299 bp open reading frame encoding 432 amino acids, protein molecular weight was 46.986 kDa and theoretical isoelectric point of 8.90.The Bet/ProT homologous genes were obtained from various plant species. The highest similarity was observed with proline transporter of Arabidopsis thaliana (AtProT3). Under salt stress, betaine gene expression was not induced significantly, but induced significantly under mannitol stress. Full-length sequence data for cDNA of the betaine transporter gene was submitted to the GenBank with accession number: KX013323.1. This study is designed to provide in-depth understanding of the roles of ZmBetProt in regulation of betaine transporter under abiotic stress.