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Expression of wit3.0 for accelerated wound contractions

A differently expressed gene wound inducible transcript 3.0 (wit3.0) was isolated from rat wounded gingival fibroblasts. Due to the unique expression pattern of this gene, we hypothesize wit3.0 expression accelerates wound contraction. The objective of this study is to investigate the potential role of wit3.0 in wound contraction. We compared the contractility of wounded/ un‐wounded gingival and skin fibroblasts by in vitro collagen gel contraction assay. wit3.0 siRNA were transfected to wounded fibroblasts to further test the function of wit3.0. In vivo function of wit3.0 was tested by transducing lentiviral vector carrying wit3.0 mixing with gelatin to wounded skin. Wound area sizes were measured, and wit3.0 gene expression and protein levels were determined. Results showed wounded gingival fibroblasts had the fastest contraction rate. This contractility was inhibited by the siRNA treatment. Transduction of wit3.0 to skin tissue also showed a faster wound closure rate. We conclude wit3.0 expression positively correlates to the cell’s contractility thus warrant further investigations of the mechanism of this gene and its potential application to skin wound healing. Source: various donors.