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Localization of calcium-dependent ATPase in germinating pollen grain and pollen tube in Vicia faba.

It was demonstrated by a cytochemical method that the calcium-dependent ATPase was present in the germinating pollen grain. Immediately after hydration the enzyme was observed in the pollen plasmalemma. During germination, the chief sites of the enzyme activity were the plasmalemma around the aperture site and the cisternae of endoplasmic reticulum. In the pollen grain with a grown tube, the enzyme was localized in the plasmalemma of the tube tip, in the endoplasmic reticulum below the tip, and in the tonoplast of the vacuoles formed in the pollen grain after tube germination.