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Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

The development of a surface plasmon resonance (SPR) spectrometer comprising angular‐resolved analysis for quasi‐monochromatic illumination is reported. The optical system utilizes disposable, injection‐molded chips combined with a lateral imaging optical system for parallel analysis of one‐dimensional spot arrays. Further parallelization is achieved by introducing a segmented light source. This source sequentially illuminates three neighbored one‐dimensional arrays in order to keep angular‐resolved analysis without introducing any mechanically moving parts. This system is applied to detect genetic variations among different DNA samples obtained from polymerase chain reaction (PCR). For this purpose, 135 spots on the chip surface have been prepared by spotting and analyzed separately.