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Caries management using calcium sensitive Casein dentifrice – An in-vitro study

The purpose of the present study was to evaluate and compare remineralization potential of novel calcium sensitive Casein, fluoridated and non-fluoridated dentifrice using Scanning Electron Microscope (SEM). An in-vitro experimental study was carried out on 45 extracted maxillary first premolars, which were collected according to the inclusion criteria and stored in 10% formalin solution followed by demineralization process. Acrylic embedded teeth blocks were prepared and randomly divided in 3 groups: Group A: fluoridated dentifrice, Group B: non-fluoridated dentifrice and Group C: novel Calcium sensitive Casein dentifrice. All the samples were subjected to remineralization process using pH cycling model for 28 days. DIAGNOdent® pen measurement were obtained at three different time intervals i.e., before demineralization, after demineralization and remineralization. Specimens were observed under SEM after demineralization and remineralization procedure. The results that were obtained on DIAGNOdent® pen measurements before and after treatment showed the Casein group was statistically significant than fluoridated group (p<0.020). Casein dentifrice showed highest remineralization, as opposed to fluoridated and non-fluoridated dentifrice and thus can be used for the management of initial carious lesion.