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Increased alpha-fetoprotein biosynthesis in rats following DL-ethionine and carbon tetrachloride injuries.

The incorporation of 14C-leucine into alpha-fetoprotein (AFP) in serum and liver extracts from DL-ethionine and carbon tetrachloride (CCl4)-injured rats was measured directly by using an immunochemical technique. The maximum value with nearly 10-fold increases of serum AFP was obtained in 3 days of both treatments. However, no significant increase in the immunoprecipitable AFP in liver extracts was detected under these conditions.