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Calibration of time-of-flight optical spectroscopy

Use of pure diffusion theory produced errors in the measurement of absorbance and scattering using a time-of- flight system. In an attempt to produce quantitative values for absorbance and scattering, we performed both software simulations and empirical collections. For the empiric correction, we first developed a calibration system based upon a homogeneous liquid test phantom. Next, we performed multiwavelength (785, 808, 850, 904 nm) time-resolved spectroscopy upon known standards using this model, and then developed an algorithm to correct for the differences between our system and the known values. We suspect that part of the difference between theory and actual measured values, in the absence of correction, may be due to the response characteristics of our diode-based laser and detection system. If so, then our calibration represents a deconvolution of the response characteristics of the system, and suggests that `less clean' components can be used, reducing cost of time-resolved systems.