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In Vitro Bulblet Formation in Dutch Iris


In vitro bulbing of Dutch iris (Irish xiphium × I. tingitana) was developed to facilitate transfer of tissue-cultured plant materials to greenhouse conditions. The environmental factors that increased bulb number, size, and weight were: 22C or higher for 6 months where there was no prechilling period and complete darkness. Bulblet formation was not enhanced when the culture medium was supplemented with auxins, cytokinins, or combinations of auxins and cytokinins. The most favorable medium constituents were 6% to 38% sucrose, full-strength Murashige and Skoog inorganics, and 0.6% to 0.8% Phytagar.