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Neratinib in Early-Stage Breast Cancer: A Profile of Its Use in the EU

Neratinib (Nerlynx®) is an oral, irreversible pan-human epidermal growth factor receptor (HER) tyrosine kinase inhibitor of HER1, HER2 and HER4. Neratinib therapy for 12 months significantly reduced the risk of invasive disease recurrence or death relative to placebo at both 2 and 5 years post-randomization in the pivotal ExteNET trial in women with early-stage HER2-positive breast cancer who had completed adjuvant trastuzumab. Subgroup analyses showed that patients with hormone receptor (HRc)-positive disease derived greater benefit with neratinib than patients with HRc-negative disease, and patients who initiated neratinib within 1 year of completing trastuzumab had better outcomes than those who started treatment 1–2 years after trastuzumab. This led to the approval of neratinib in the EU as extended adjuvant therapy for patients with early-stage HRc-positive, HER2-positive breast cancer and who are less than 1 year from completion of prior adjuvant trastuzumab-based therapy. It is the first agent of its class to be approved in the EU in this setting. As with other tyrosine kinase inhibitors, diarrhoea, which was manageable with antidiarrhoeal prophylaxis and/or dose modifications, was the most common any-grade or grade ≥ 3 treatment-emergent adverse event with neratinib. Thus, current evidence indicates that neratinib provides a valuable option to reduce the risk of recurrence in this setting and has been included in the updated ESMO patient guide as an extended adjuvant therapy for some patients.


What is the Rationale for Developing
Neratinib?
A better understanding of the complex molecular heterogeneity of breast cancer over the last several decades has led to the development of targeted therapies, such as the antihuman epidermal growth factor receptor 2 (HER2) monoclonal antibody trastuzumab, a mainstay in the treatment of patients with HER2-positive disease [1]. HER2 (ErbB2) is a member of the ErbB family of tyrosine kinase receptors, consisting of the epidermal growth factor receptor (EGFR, ErbB1, HER1), ErbB3 (HER3) and ErbB4 (HER4) [2]. ErbB receptor signalling plays a key role in the regulation of cell proliferation, survival, differentiation and migration [2]. Deregulation of signalling via these receptors (primarily HER1 and HER2) has been implicated in several types of cancers, including breast cancer [2]. HER2 is overexpressed in approximately 20-30% of breast cancer tumours and is associated with more aggressive disease, higher recurrence rates and increased mortality [1].
In early-stage HER2-positive breast cancer, standard adjuvant therapy with chemotherapy and trastuzumab for 1 year was associated with an absolute improvement of 6-12% in disease-free survival and 6-9% in overall survival during longer-term follow-up (8-12 years); however, 15-20% of patients had disease recurrence [3]. Several options have been explored to escalate standard adjuvant therapy, including extending trastuzumab treatment to 2 years and combining trastuzumab with lapatinib (dual HER1 and HER2 inhibitor) or bevacizumab (anti-vascular endothelial growth factor monoclonal antibody) [3,4]. However, no significant clinical benefit was demonstrated with these treatments [3]. The search for alternative therapies led to the development of the oral, irreversible, pan-HER tyrosine kinase inhibitor neratinib (Nerlynx ® ) as an extended adjuvant treatment for patients with early-stage, HER2-positive breast cancer who have completed trastuzumab-based adjuvant therapy [4,5]. This review focuses on the clinical evidence for its use in the extended adjuvant setting, with a particular focus on the patient population for which it is approved in the EU, i.e. patients with hormone receptor (HRc)-positive, HER2positive tumours who initiate neratinib < 1 year from completing trastuzumab-based adjuvant therapy [6]. Neratinib is the first anti-HER2 agent to be approved in the EU in this indication [7].

How Does Neratinib Work?
Neratinib binds irreversibly to a conserved cysteine residue in the ATP-binding pocket of HER1, HER2 and HER4, and reduces receptor autophosphorylation, resulting in sustained inhibition of downstream signal transduction events and cell cycle regulatory pathways, leading to cell cycle arrest and apoptosis [8]. Neratinib is highly selective for the ErbB family members and does not significantly inhibit several other kinases, including Akt, cyclin D1/cdk4, c-Raf and c-met [8]. In preclinical studies, neratinib inhibited the proliferation of HER1-and/or HER2-overexpressing cell lines, including breast cancer and epidermal carcinoma cell lines, and inhibited tumour growth in several HER1-and HER2-dependent xenograft models [8,9].
By simultaneously inhibiting multiple members of the ErbB family, neratinib has the potential to overcome trastuzumab resistance mechanisms, such as compensatory crosstalk between receptors [10]. Indeed, neratinib was shown to be growth inhibitory in HER2-positive breast cancer cell lines with innate and acquired resistance to trastuzumab [9]. Furthermore, combination treatment with neratinib and trastuzumab had an additive effect in trastuzumab-sensitive cell lines and in cell lines with acquired resistance to trastuzumab, as indicated by significantly greater inhibition of proliferation with the combination than with each agent alone [9]. This improved activity of neratinib in combination with trastuzumab may be due to the different mechanisms of action of trastuzumab (binds to the extracellular domain of HER2 and induces ligand-dependent activation of HER receptors) and neratinib (binds to the intracellular ATP-binding site and inhibits receptor autophosphorylation) [11]. An additive tumour growth inhibitory effect of neratinib plus trastuzumab was also seen in a xenograft model of HER2-positive breast cancer sensitive to trastuzumab [9].
Oestrogen signalling plays a key role in breast carcinogenesis, and crosstalk between the oestrogen receptor (ER) and HER2 has been implicated in the development of resistance to endocrine-and HER2-targeted therapies [12]. The inhibition of HER2 signalling by targeted therapies is believed to activate/restore ER signalling, providing an escape mechanism for the tumour cells and resulting in resistance to HER2-targeted therapies [12,13]. Endocrine therapy can also activate HER2 signalling and lead to resistance to HER2-directed therapy [12]. Thus, combining ERand HER2-targeted therapies to inhibit both signalling pathways simultaneously has the potential to improve clinical outcomes ( Fig. 1). Indeed, a preclinical study showed that neratinib plus fulvestrant but not fulvestrant alone maintained complete tumour responses in mice with ER-positive, HER2-positive xenografts that had responded to trastuzumab plus paclitaxel [14]. In the clinical setting, the benefit of 12 months' extended adjuvant treatment with neratinib in the pivotal ExteNET trial in patients with early-stage HER2positive breast cancer was seen largely in the subgroup of patients with HRc-positive disease [13], the majority (93%) of whom were receiving concomitant endocrine therapy [15] (Sect. 4).

For Whom is Neratinib Indicated?
In the EU, neratinib is indicated for the extended adjuvant treatment of adults with early-stage HRc-positive, HER2overexpressed/amplified breast cancer and who are < 1 year from the completion of prior adjuvant trastuzumab-based therapy [6]. Table 1 provides a summary of the EU prescribing information for neratinib in this indication.

What is the Clinical Efficacy of Neratinib?
The large (n = 2840), randomized, double-blind, multicentre, placebo-controlled, phase 3 ExteNET trial assessed the efficacy of oral neratinib as extended adjuvant therapy in patients with early-stage HER2-positive breast cancer who had completed adjuvant therapy with trastuzumab [15]. The trial included patients aged ≥ 18 years (≥ 20 years in Japan) with locally (and subsequently centrally confirmed in 60% of primary tumour specimens) invasive HER2-positive breast cancer stage 1-3 (later amended to stage 2-3; Fig. 2) without evidence of recurrence. Patients had to have completed ≤ 2 years' trastuzumab-based therapy (amended later to 1 year; Fig. 2) before randomization, have an Eastern Cooperative Oncology Group (ECOG) performance status score of 0 or 1, normal organ function and a left ventricular ejection fraction (LVEF) within normal range [15]. HRc status (assessed locally) had to be known and it was recommended that patients receive concurrent adjuvant endocrine therapy for HRc-positive disease [15,16].
Patients received neratinib (240 mg once daily) or placebo for 12 months, unless there was disease recurrence, new breast cancer, an intolerable adverse event (AE) or withdrawal of consent. Randomization was stratified according to HRc status (positive or negative), nodal status (0, 1-3 or ≥ 4) and trastuzumab adjuvant regimen (given sequentially or concurrently with chemotherapy) [15]. The median time from the last trastuzumab dose to randomization was 4.4 and 4.6 months in the neratinib and placebo groups, respectively; 2297 (80.9%) patients had completed trastuzumab therapy within 1 year of initiating study treatment. Demographic and disease characteristics at baseline were generally similar between the neratinib and placebo groups. At baseline, across the two groups, the median age of patients was 52 years; 57% had HRc-positive disease (i.e. ER-and/ or progesterone receptor-positive); 32, 40 and 9% of patients had stage T1, T2 and ≥ T3 tumours, respectively; previous trastuzumab treatment was administered concurrently with chemotherapy in 62% and sequentially in 38% of patients; 24, 47 and 30% of patients had 0, 3 and ≥ 4 positive nodes, respectively. In patients with HRc-positive disease, 93% were receiving concomitant endocrine therapy [15].
The primary endpoint was invasive disease-free survival (DFS; see Table 2 for definition) assessed in the intent-totreat population [15]. Primary efficacy analyses were conducted at 2 years (cut-off date July 2014), a sensitivity analysis of efficacy outcomes was conducted at 5 years (cut-off date March 2017) and an analysis of OS will be undertaken after 248 events have occurred [15,16]. As most patients had completed the trial at 2 years after randomization, reconsent from patients was required for extended follow-up; 2117 patients re-consented to retrospective collection of data between 2 and 5 years, and survival data beyond year 5 [15].

Intent-to-Treat Population
Neratinib treatment for 12 months significantly reduced the risk of invasive disease recurrence or death relative to placebo in women with early-stage HER2-positive breast cancer who had completed adjuvant trastuzumab therapy (Table 2) [6,15]. At 2 years (primary endpoint) [6,15] and at the 5-year follow-up [16], neratinib recipients in the intent-to-treat population had significantly higher rates of invasive DFS than placebo recipients, corresponding to a 34 and 27% relative reduction in the risk of invasive disease recurrence or death at the two timepoints ( Table 2). A separation between the Kaplan-Meier curves for neratinib and placebo was seen at ≈ 3 months and was maintained throughout the 5-year follow-up period [16]. DFS including ductal carcinoma in situ was also significantly improved with neratinib relative to placebo at 2 and 5 years, but there were no significant differences between the neratinib and placebo groups for distant DFS, time to distant recurrence or cumulative incidence of CNS recurrence at these timepoints (Table 2) [6]. Overall survival data are not yet mature [15,16].
Health-related quality of life (HR-QOL) deteriorated slightly with neratinib during month 1, as assessed by the difference in adjusted mean change from baseline versus placebo in the Functional Assessment of Cancer Therapy-Breast (FACT-B) total scores and EuroQol 5-Dimensions (EQ-5D) visual analogue scale scores, but this was not considered clinically important [15]. In both treatment groups the two HR-QOL scores recovered towards baseline scores after the first month and the between-group differences became less pronounced [15].
In subgroup analyses at 2 and 5 years, improvements in invasive DFS consistent with those in the intent-to-treat population were seen in almost all subgroups (n = 101-2297), regardless of demographic and disease characteristics (HRs < 1; p interaction at 5 years nonsignificant for all subgroups) [15,16]. A prespecified subgroup analysis of invasive DFS at 2 years in patients stratified according to HRc status suggested significant benefit with neratinib relative to placebo in 1631 patients with HRc-positive disease [95 vs.

HRc-Positive Patients Treated ≤ 1 Year After Trastuzumab
An exploratory analysis showed that the subgroup of patients in ExteNET who had HRc-positive, HER2-positive disease and had received neratinib ≤ 1 year from completion of trastuzumab (n = 1334) derived the greatest benefit with neratinib, as indicated by a 51% reduction in the risk of invasive disease recurrence or death at 2 years [6,17], with the treatment benefit maintained at the 5-year followup [16,17] ( Table 2). The Kaplan-Meier curves for invasive DFS for neratinib and placebo recipients separated at ≈ 3 months and remained separate throughout the 5-year follow-up period [17]. DFS including ductal carcinoma, distant DFS and time to distant recurrence were also significantly improved with neratinib relative to placebo at 2 and 5 years post-randomization (Table 2). Although the cumulative incidence of CNS recurrence was numerically lower with neratinib than with placebo both at 2 and 5 years, the between-group difference did not reach statistical significance (Table 2) [16,17].

What is the Tolerability of Neratinib?
Neratinib had an acceptable safety profile in the large ExteNET trial in patients with early-stage HER2-positive breast cancer who had completed adjuvant trastuzumab therapy [15,16]. The most common any-grade or grade ≥ 3 treatment-emergent AE with neratinib was diarrhoea (Fig. 3) [15]. Of note, antidiarrhoeal prophylaxis was not specified in the study protocol, instead treatment for diarrhoea was advised once symptoms became apparent. Other grade 3 or 4 AEs each occurred in < 4% of patients in the neratinib group, with non-gastrointestinal events generally occurring at similar incidences in neratinib and placebo recipients. QT prolongation was reported in 3% of neratinib and 7% of placebo recipients and grade ≥ 2 decreases in LVEF in 1% of patients in each group. Interstitial lung disease occurred in two patients in the neratinib group and one patient in the placebo group, pulmonary fibrosis in one and two patients, respectively, and pneumonitis in one patient in each group; second cancers (neoplasms benign, malignant and unspecified, including cysts and polyps) occurred in 1% of patients in each group [15].

Amendment 3 (Pfizer, Feb 2010)
Patients with stage 2-3 BC at higher risk of recurrence, i.e. those with node-positive disease and randomized ≤ 1 year after TRA

Amendment 9 (Pfizer, Oct 2011)
Stop enrolment of new subjects and limit the follow-up period to 2 years post-randomization

Amendment 13 (Puma Biotechnology, Oct 2013)
Extend the collection of recurrent disease events and deaths to 5 years post-randomization and conduct long-term survival follow-up  [15]. Treatment-emergent AEs resulted in dose reductions in 31% and 2% of patients in the neratinib and placebo groups, and treatment discontinuations in 28% and 5% of patients [16]. At the time of the 2-year analysis, four deaths in the neratinib group and three deaths in the placebo group unrelated to disease progression were reported after treatment discontinuation, none of which were considered to be drug related [15]. At the time of the 5-year analysis, 121 deaths had occurred in the neratinib and placebo groups combined (data are masked), of which 102 deaths were because of disease progression and 19 deaths were due to other reasons [16]. A review of unsolicited serious AEs occurring > 28 days after the last dose of the study drug reported to a safety database showed no evidence of increased long-term toxicity with neratinib relative to placebo, particularly symptomatic cardiac toxicity or second primary malignancies [16].
The safety profile of neratinib in the subgroup of patients with HRc-positive, HER2-positive breast cancer who initiated neratinib ≤ 1 year from completion of trastuzumab was generally similar to that in the overall safety population [17]. The most common grade ≥ 3 treatment-emergent AEs with neratinib included diarrhoea (39% vs. 1% with placebo), fatigue (2% vs. < 1%) and nausea (1% vs. < 1%). Treatment-emergent AEs resulted in dose reductions in 31% of neratinib and 2% of placebo recipients, dose holds in 42% and 11%, and hospitalizations in 6% and 5% of patients, respectively [17].

Diarrhoea
Diarrhoea was the main toxicity associated with neratinib in ExteNET (Fig. 3), leading to dose reductions in 26% (vs. 1% in placebo recipients), hospital admissions in 1% (vs. < 1%) and discontinuation of therapy in 17% (vs. < 1%; after median 20 vs. 241 days) of neratinib recipients in the absence of antidiarrheal prophylaxis [15]. Most grade 3 diarrhoea events with neratinib were reported during the first month (cycle 1) of treatment, with a median time to onset of 8 days, median cumulative duration of 5 days/patient and median duration of 2 days/event [15], supporting the use of preventive management with antidiarrheal prophylaxis early during neratinib treatment. In pooled data from 1660 patients who received neratinib monotherapy without loperamide prophylaxis, 95% of patients had at least one episode of diarrhoea, and diarrhoea was the most common reason for treatment discontinuation (14%); 25% of patients required dose reductions because of diarrhoea [6]. Grade 3 diarrhoea occurred in 38% of neratinib recipients in this analysis, and grade 4 diarrhoea in 0.2% of patients; 2% of patients required hospitalization as a result of diarrhoea [6].
To manage neratinib-associated diarrhoea, it is recommended that antidiarrhoeal prophylaxis be initiated with the first dose of neratinib and continued for the first 1-2 months of therapy [6]. Dosage adjustment or temporary discontinuation of neratinib may also be required to manage diarrhoea (Table 1) [6].
An international, open-label, sequential-cohort, phase 2 CONTROL (PUMA-NER-6201) study is assessing the effects of loperamide prophylaxis alone or with add-on budesonide (a locally acting corticosteroid used for inflammatory gastrointestinal conditions) or colestipol (a bile acid sequestrant) on neratinib-associated diarrhoea [18]. The study enrolled adults who had HER2-positive stage 1-3c breast cancer and had completed trastuzumab-based adjuvant therapy or had discontinued treatment as a result of AEs, with the last dose of trastuzumab > 2 weeks and ≤ 1 year prior to enrolment. All patients received neratinib 240 mg/day in 28-day cycles for 1 year (13 cycles); in addition, patients received loperamide prophylaxis for 1 or 2 cycles and then as needed (4 mg initial dose, then 6-12 mg/ day; n = 137), loperamide with add-on budesonide (9 mg once daily for 1 cycle; n = 64) or loperamide with add-on colestipol (2 g twice daily for 1 cycle; n = 120). At the time of an interim analysis (cut-off date November 2017), the median duration of neratinib therapy was 11.5, 11.9 and 3.7 months in the loperamide, budesonide and colestipol cohorts, respectively. The primary endpoint was the incidence of grade ≥ 3 diarrhoea [18].
Concomitant antidiarrhoeal treatment appeared to reduce the duration, incidence and severity of diarrhoea in patients receiving neratinib in CONTROL [18]. In terms of grade ≥ 3 diarrhoea, add-on colestipol was associated with the greatest reduction in the incidence relative to the other loperamide cohorts or the ExteNET trial as the historical control (11,27 and 31% in the colestipol, budesonide and loperamide cohorts vs. 40% in ExteNET). Grade 1 or 2 diarrhoea occurred in 49, 59 and 56% of patients in the loperamide, loperamide + budesonide and loperamide + colestipol cohorts in CONTROL compared with 55% of patients in ExteNET; no diarrhoea was reported in 20, 14 and 33% of patients in the respective cohorts compared with 5% of patients in ExteNET [18].
The median cumulative duration of any-grade diarrhoea in all three loperamide cohorts (loperamide, loperamide + budesonide and loperamide + colestipol) in CON-TROL appeared to be shorter (any grade: 14, 24 and 16 vs. 59 days in ExteNET) and the proportions of patients requiring dose reductions (7, 3, 4 vs. 26%) or dose holds (15, 19 and 9 vs. 34%) because of diarrhoea appeared to be lower than those in the ExteNET trial [18]. The CONTROL study cohorts also had reduced incidences and severity of diarrhoea relative to ExteNET during the course of neratinib therapy. In ExteNET, grade 2 and 3 diarrhoea had the greatest incidence during cycle 1 (month 1) and was still occurring during months 2-12 (chronic profile). In comparison, in the CONTROL study cohorts, the incidence of grade 2 or 3 diarrhoea was reduced during cycle 1 (month 1) and in months 2-12 [18].

What Conclusions can be Made Regarding the Clinical Use of Neratinib?
In the EU, the standard treatment for early-stage breast cancer in most cases is surgical resection with or without radiation therapy, and/or systemic adjuvant therapy [19]. The choice of systemic therapy is based on the surrogate intrinsic phenotype determined by HRc and HER2 status and assessment of the proliferation marker Ki67 [19]. For patients with HER2-positive disease, the anti-HER2 monoclonal antibody trastuzumab has become the mainstay of treatment, with current guidelines recommending trastuzumab therapy for 1 year in combination with chemotherapy [1]. For patients at high risk of recurrence, the anti-HER2 monoclonal antibody pertuzumab has been approved as neoadjuvant and adjuvant therapy in combination with trastuzumab and chemotherapy [20]. In addition, patients with HRc-positive disease are administered endocrine therapy for at least 5 years [1]. Despite the improved clinical outcomes with trastuzumab, the issue of high recurrence rates remains, which has spurred the development of new therapies. One such agent is the  Fig. 3 Any-grade adverse events occurring at an incidence of ≥ 20% with neratinib (n = 1408) and placebo (n = 1408) as extended adjuvant therapy in the ExteNET study. AEs adverse events irreversible, pan-HER tyrosine kinase inhibitor neratinib, which has been developed as extended adjuvant therapy to reduce the risk of recurrence in patients with HER2-positive early-stage breast cancer. Treatment with neratinib for 12 months significantly improved the primary endpoint of invasive DFS relative to placebo at both 2 and 5 years in the pivotal ExteNET study in women with early-stage HER2-positive breast cancer who had completed adjuvant trastuzumab (Sect. 4.1). The secondary endpoint of DFS including ductal carcinoma in situ was also improved and HR-QOL was not adversely affected by neratinib relative to placebo (Sect. 4.1). It has been suggested that the improved clinical outcomes with neratinib as adjuvant therapy could be due to irreversible kinase inhibition through the binding of neratinib to a cysteine residue in the ATP-binding pocket, resulting in more effective blockade of HER2 with neratinib than with trastuzumab [15].
Notably, subgroup analyses of ExteNET suggested that patients with HRc-positive disease derived greater benefit with neratinib than patient with HRc-negative disease, and patients who initiated neratinib ≤ 1 year of completing trastuzumab had better outcomes than those who started treatment 1-2 years after trastuzumab (Sect. 4.1). Subsequent analyses showed that patients with HRc-positive disease who received treatment ≤ 1 year after trastuzumab derived the greatest treatment benefit, with significant improvement in invasive DFS and other efficacy outcomes, apart from the cumulative incidence of CNS recurrence, for which event numbers remained small (Sect. 4.2).
It has been suggested that the improved efficacy in patients with HRc-positive disease may be because of dual inhibition of ER-HER2 crosstalk by neratinib and endocrine therapy (which was administered to the majority of patients in this subgroup). Another explanation may relate to the timing of adjuvant neratinib therapy and the different clinical course of HRc-negative and HRc-positive subgroups in terms of the risk of recurrence [13]. HRc-negative patients are at highest risk of recurrence in the first few years after completion of adjuvant trastuzumab, while HRc-positive patients are at a long-term continuous risk of recurrence [21]. In patients with HER2-positive breast cancer who were disease-free after completion of prior adjuvant chemotherapy and who were not treated with anti-HER2 therapy (placebo arm of the TEACH trial), the mean risk of recurrence in years 1-5 was 9% per year in patients with HRc-negative disease compared with 5% per year for patients with HRc-positive disease (p = 0.002) [22]. The high risk of recurrence in patients with HRc-negative disease declined sharply thereafter, such that there was no significant difference between the HRc-positive and -negative subgroups in years 6-10 (mean risk of 4% per year in both groups) [22]. This suggests that there is a longer window of opportunity to provide benefit with extended adjuvant therapy in patients with HRc-positive disease [13].
Neratinib had a generally acceptable safety profile in ExteNET, with the most common any-grade or grade ≥ 3 treatment-emergent AE being diarrhoea, which is considered a drug class effect [16]. The highest incidence of diarrhoea with neratinib was in cycle 1 of treatment, which may explain the decrease in HR-QOL scores during this period [15]. The drug of choice for the management of diarrhoea associated with tyrosine kinase inhibitors is the opiod loperamide, as it has local activity in the gut and is minimally absorbed and thus not associated with systemic effects [23]. Indeed, intensive loperamide prophylaxis at the start of neratinib therapy in phase 1 and phase 1/2 studies was associated with improved tolerability of neratinib, as indicated by fewer grade 3 diarrhoea events in patients receiving intensive loperamide prophylaxis (incidence 0-17%) compared with no or suboptimal loperamide prophylaxis (incidence 30-53%) [24]. In ExteNET, treatment with loperamide was initiated only after the development of symptoms, and grade 3 diarrhoea was reported in 40% of neratinib recipients (Fig. 3). Because of the risk of severe diarrhoea with neratinib, prophylactic antidiarrhoeal treatment is recommended, and should be initiated with the first dose of neratinib and continued for 1-2 cycles [6]. Interim results from the ongoing, phase 2 CONTROL study suggest that the addition of colestipol to loperamide may further reduce the incidence and duration of grade ≥ 3 diarrhoea (Sect. 5.1). Neratinib was not associated with long-term toxicity in a safety database review, particularly symptomatic cardiac toxicity or second primary malignancies (Sect. 5).
The ExteNET study protocol underwent several amendments during the course of the study as three different sponsors assumed responsibility (Fig. 2), including cessation of enrolment and shortening of follow-up to 2 years, and subsequent restoration of follow-up to 5 years [15,16]. As a result, reconsent for long term follow-up was required for most patients and, because of missing data, there is some uncertainty regarding the magnitude of effect at 5 years [13,16]. However, the effect size observed at the 5-year follow-up suggests a clinically significant benefit in HRc-positive patients who received neratinib ≤ 1 from completion of trastuzumab [13].
Given its efficacy and manageable safety, neratinib was recently approved as extended adjuvant treatment of patients with early-stage HRc-positive, HER2-positive breast cancer who are < 1 year from the completion of trastuzumab therapy [6]. Although the exact position of neratinib in the treatment algorithm for early-stage breast cancer (for example in relation to adjuvant pertuzumab therapy) remains to be determined [25], neratinib provides a valuable treatment option to reduce the risk of recurrence in these patients. The recently updated ESMO patient guide also includes neratinib as an extended adjuvant therapy for some patients following the completion of trastuzumab therapy [26].