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A radiochromatographic method for biliary contrast agent metabolites.

This paper describes a method which may be used to determine conjugation of iodipamide and ipodate in plasma, bile and urine. Using ‘“I-labelled contrast agents, conjugation was determined by thin-layer chromatography, before and after beta-glucuronidase incubation of samples. This method achieves a high degree of sensitivity. It was found that ipodate is excreted in dog bile principally as the glucuronide conjugate. Excretion of a small amount of iodipamide as a glucuronide conjugate was also detected.