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A Microchip with Air Sampling and Chemiluminescence Detection for Analyzing Iron in Nature Water and in Whole Blood

Abstract A mircrofluidic chip, based on using an air stream for fluidic controlling and coupled with chemiluminescence (CL) detection, was presented. The reported design promised a simple, low cost chip fabrication, and an easy operation. With each assay time less than 3 min, the described microfluidic chip was applied in determination of iron(II) content in nature water and total iron content in whole blood. Experiment results showed the potential for a multitude of applications of the presented chip. The calibration graph of chemiluminescent intensity (I CL) vs. iron(II) concentration was linear in the range 1.0 × 10−6–5.0 × 10−5 mol L−1, and the detection limit (DL) was 3.0 × 10−7 mol L−1 (3σ).