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The influence of feeding laying hens N-3 and N-6 fatty acids sources on blood antioxidants status: A comparative study

The objective of this study was to determine the effects of dietary supplementation with different fat sources on blood oxidative status parameters. Seventy five (Hisex) bird, 20 week old, were obtained from Animal Production Research Center (kuku), divided into three groups (n=25) for each one. The control group (A) was under control diet based on corn, maintains the (NRC, 1994) requirements for laying hens, the experimental groups, supplemented by 10% flax seeds (B), and 10% sunflower seeds (C), added to the diet. The trial continued for eight weeks, blood samples were collected once per month (week 4 and 8), in EDTA coated vials, immediately placed into iced-container, centrifuged at 3000rpm/20 min. Samples were separated in aliquot, and stored at -20 o C and -80 o C until analysis. Plasma analysis for enzymatic and non-enzymatic antioxidants, revealed no significant different level of uric acid, malondialdehyde (MDA) and vitamin C, between the control group and the treated groups, while catalase (CAT), superoxide dismutase (SOD), and vitamin A, levels were significantly enhanced by the addition of 10% flax seeds and 10% sunflower seeds. Vitamin E concentration was significantly elevated in group (C), compared to the control group (A) and group (B).