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Genetic background for moderate resistance against fusarium head blight among winter wheat developed in the Forrest Steppe of Ukraine

Aim. The aim of the work was to evaluate the genetic background of resistance to Fusarium head blight in common winter wheat cultivars based on the allelic state of the TDF_076_2D gene conferring tolerance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Methods. We studied 91 winter common wheat cultivars developed in the Institute of Plant Physiology and Genetics of NAS of Ukraine. A silica-based commercial kit was used for DNA extraction. For the allelic state detection, the INDEL1 marker co-segregating with the TDF_076_2D gene was used. Results. The frequency of the resistance allele according to the marker for the gene conferring moderate resistance to the Fusarium fungi made up 0.802. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the gene of interest. The data obtained are consistent with the results of previous research for the wider sample of the winter and spring common wheat cultivars. The cultivars with confirmed resistance allele might show lower infection level in the field and serve as a source of the gene in marker assisted selection. 
Keywords: common wheat, disease resistance genes, Fusarium head blight, molecular markers.