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Toxic Effects of CDNB on MDA in Liver of Freshwater Fish, Brocarded Carp

There were few reports on the lipid peroxidation of aquatic organisms exposed to 2,4-Dinitrochlorobenzene(CDNB). This research explored the hepatic lipid peroxidation of fish to long-term exposure of CDNB for the first time. Freshwater fish Brocarded Carp were chosen as experimental animals. The fish were exposed to four different concentrations of CDNB (0.168-0.5 mg/l) for 15 days and then liver tissues were separated for determination. As shown from the results, 15 days afterwards, the MDA level was induced significantly on the whole compared to control group; the MDA content responded to CDNB exposure in all treatment group; The MDA content were also determined to analyze the occurrence of oxidative stress. Besides, good dose-effect relations, which cover most of the exposure concentration range, were found between MDA level and CDNB exposure concentration. In conclusion, MDA level in the liver of fish may be potential early biomarkers of CDNB contamination in aquatic ecosystems, and further studies will be necessary.