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Direct measurement of HDL cholesterol: Method eliminating apolipoprotein E‐rich particles

It has been reported that the existing direct method of high density lipoprotein (HDL) cholesterol measures particles enriched with apolipoprotein E (apoE). The aim of our study was to investigate a new analytical protocol to directly measure HDL cholesterol that eliminates apoE‐rich particles. The interactions of four lipoproteins (HDL3, HDL2, LDL, and VLDL + chylomicron) with surfactants, divalent cations, sugars, and lectins were investigated. By analyzing sera, HDL3, and HDL2, we examined the relationships among the measurements obtained by our protocol, a precipitation method using heparin‐MnCl2, and a commercially available kit for this direct method. A significant difference was found between the direct method and the heparin‐MnCl2 method, but not between our protocol and the heparin‐MnCl2 method. Multiple regression analysis showed that the difference between the direct method and the heparin MnCl2 method is dependent on sources of apoE‐rich HDL. In conclusion, our protocol enables a direct measurement of HDL cholesterol that eliminates apoE‐rich particles. J. Clin. Lab. Anal. 15:223–229, 2001. © 2001 Wiley‐Liss, Inc.