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The biological properties of cancer stem cells in malignant gliomas in tissue culture: a review

This review presents recent data on the biological properties of cancer stem cells in brain gliomas. The possibilities of using of experimental methods of cell culture and immunohistochemical identification of tumor stem cells as a part of neurospheres (tumorospheres) are shown. Methodological features of neurospheres culture are described.Literature data about the dependence of neurospheres formation activity in the culture on the degree of anaplasia of original glioma tissues are presented considering the disease outcomes in neuro-oncological patients. Possibilities of the practical use of cultured tumorospheres in the study of selective impact of antiblastic drugs on tumor stem cells in gliomas are described.