{'fraction_non_alphanumeric': 0.033720287451630734, 'fraction_numerical': 0.01170075548185001, 'mean_word_length': 5.635085574572127, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
PASH Syndrome: Comparing Pathogenic Mechanism of Pyoderma Gangrenosum, Acne and Suppurative Hidradenitis

PASH syndrome is a recently described autoinflammatory disease, consisting of pyoderma gangrenosum (PG), acne and hidradenitis suppurativa (HS). We report a new case of PASH syndrome, highlighting the features of the rare condition. For a better understanding of each of the three diseases and of the PASH syndrome, we reviewed the common and the particular aspects of these pathologies, looking from different angles of inflammation mechanisms. Among the approached topics, we discussed about: increased production of interleukin-1 (IL1) and of other cytokines, altered matrix metalloproteinases expression, multiple roles of neutrophils, genetic profiling and inflammatory infiltrates. As far as we know it is the first time we note the similarities and differences of pathways beyond the neutrophils mediated cutaneous manifestations present in PASH, PG, HS and acne. Our findings emphasize that PASH syndrome may be an inspiring lesson for rethinking autoinflammatory, neutrophilic, innate immune skin diseases. Introduction PASH syndrome is a neutrophil mediated inflammatory syndrome, with recurrences and relapses, whose management represents a real challenge [1]. The acronym PASH stands for pyoderma gangrenosum (PG), severe acne (A) and suppurative hidradenitis (SH) [2,3]. This entity was first described in 2012 by Braun-Falco et al, who observed a different association of diseases than the one previously known as PAPA syndrome (pyogenic sterile arthritis, pyoderma gangrenosum and severe acne) [2]. A gathering of pyogenic sterile arthritis, pyoderma gangrenosum, acne and hidradenitis suppurativa is known as PAPASH syndrome [3,4] whilst the term “PsaPASH syndrome” was proposed for describing the association of psoriatic arthritis and PASH syndrome [1]. Pyoderma gangrenosum (PG) is a neutrophilic dermatosis, clinically characterized by pustules located especially over the


Introduction
PASH syndrome is a neutrophil mediated inflammatory syndrome, with recurrences and relapses, whose management represents a real challenge [1]. The acronym PASH stands for pyoderma gangrenosum (PG), severe acne (A) and suppurative hidradenitis (SH) [2,3]. This entity was first described in 2012 by Braun-Falco et al, who observed a different association of diseases than the one previously known as PAPA syndrome (pyogenic sterile arthritis, pyoderma gangrenosum and severe acne) [2]. A gathering of pyogenic sterile arthritis, pyoderma gangrenosum, acne and hidradenitis suppurativa is known as PAPASH syndrome [3,4] whilst the term "PsaPASH syndrome" was proposed for describing lower extremities, which soon become painful erythematous or violaceous ulcerations, with undermined border. It can accompany a hematologic malignancy or a chronic intestinal disease. Pathology report may reveal findings that are not specific: the dermis contains neutrophilic infiltrates (which may be arranged in abscesses or around perivascular lymphocytic infiltrates) and edema. Other disorders, such as bacterial infections (including Mycobacteria), insect bites, venous ulcers, vasculitic processes, self-induced lesions, neoplasm must be ruled out [5].
Acne, a skin disorder affecting the pilosebaceous unit, manifests clinically as papules, pustules, open and closed comedones. Acne is the most common inflammatory dermatosis in the world [6].
Sometimes, it can take a more severe form, which associates nodules, cyst, sinus tracts, accesses and which tends to scar.

Suppurative Hidradenitis (SH) is a recurrent chronic
inflammatory condition of follicular hair follicles from areas with apocrine glands, most commonly the axillary, inguinal and anogenital region. Clinical lesions can be found in intertriginous areas as suppurative nodules, abscesses, draining sinuses which heal with extensive scars [8][9]. Harley classification consisting of 3 different stages according to the severity of the lesions is used in assessing a patient with hidradenitis suppurativa. It can appear isolated or in the context of various syndromes (PASH/ PAPASH/ follicular occlusion syndrome, etc.) [9]. It was observed that HS in the context of an auto inflammatory syndrome has a poor response to treatment and a recalcitrant behavior [9]. We here present a new case of PASH syndrome and review by comparison the three mentioned multifactorial entities that define the hereditary autoinflammatory syndrome.

Case
We report the case of a 37-year-old patient, who presented for a pustule located over the left leg, evolving for two days. He      Body temperature remained within normal ranges throughout the period. He was advised to stop the previous antibiotic treatment and he was prescribed amoxicillin-clavulanate (1 g every 12 hours) and cyprofloxacyn (500 mg every 12 hours). After 7 days of antibiotic therapy, he returned to our clinic, with the abscess being replaced by a deep and painful ulcer, with undermined, violaceous borders, very suggestive of pyoderma gangrenosum.

Discussion
Each of the conditions that define PASH syndrome has a relevant psychosocial impact, affecting quality of life, being associated with increased risk for social isolation, anxiety, depression, to suicidal ideation [6].For a better understanding of each of the three diseases and of the PASH syndrome, we aimed to highlight the common and

Pathophysiology -inflammation is the key?
Acne and suppurative hidradenitis are adnexal diseases, both of which involve multifactorial damage of the pilosebaceous unit, while pyoderma gangrenosum involves a vascular neutrophilic reaction that may be folliculocentric. Polymorphous clinically, the classic aspects of these conditions are often characterized by the presence of pustules, inflammatory nodules, exudation, with consequences to disfigurement and functional impotence. Impaired immune responses -tissue and circulatory evidence Defining pathophysiological elements for acne are follicular hyperkeratinization, sebum altered production and inflammation [11]. In classical vision, acne is explained by the following events: increased activity of sebaceous glands and hyperseborrhea; abnormal follicular differentiation associated with hyperkeratinization; microbial colonization of the follicular canal followed by innate immune system activation and inflammation [6].
There is still a controversy over the temporal sequence between follicular hyperkeratinization and the onset of inflammation in acne [12]. Further studies have shown that increased activity of IL-1 actually activates keratinocytes before follicular hyperproliferation occurs [13]. So, if it was clear that follicular rupture triggers inflammation in acne, it remains to be elucidated to what extent follicular rupture actually occurs as a consequence of previous inflammation.
HS is a chronic recurrent inflammatory disease, with the origin in the hair follicles of the anatomical zones with apocrine glands.
The inflammatory stimulus is the breakage of the hair follicle and the release of keratin and bacteria in the dermis [14]. Hair follicles occlusion with subsequent rupture, destruction of the pilo-sebaceo-apocrine unit and massive secondary inflammation form painful nodules that can progress to superficial abscesses and subcutaneous extension with chronic suppurative sinus tracts and fistula that heal with vicious scars [15][16][17]. Other typical HS lesions are open comedones, usually clustered, that communicate with each other, formed by the destruction of the deep component of the pilosebaceous unit [6,18,19]. Closed comedones formed by a more superficial damage in the pilosebaceous unit, such as those seen in acne, where are not typically described in HS [20]. Impaired innate immune response also plays a role in HS. Among the evidence of innate immune system impairment are studies that have shown: increased production of IL-1β and (TNF) -α in cell cultures of affected skin [21]; IL-2 level elevated in the serum of HS patients [22]; increased expression of IL-12, IL-17, IL-23 in the skin with HS lesions [23], etc. The pathogenesis of pyoderma gangrenosum is undetermined, although there is different hypothesis about innate and acquired immunity abnormalities [24]. As in acne, suppurative hidradenitis and PASH syndrome, there is evidence of increased IL-1 activity: cutaneous and serum overexpression of IL-1β, favorable treatment under anti-IL1-β antibody therapy, frequent association with inflammatory systemic diseases [10].
An altered expression of matrix metalloproteinases was observed in each of the three diseases that define PASH syndrome, suggesting a pathogenic role. Mozeika et al obtained elevated levels of metalloproteinase-2 (MMP-2) in keratinocytes, dermal cells, hair follicles units and sinus tracts in HS [25]. In PG patients, overexpression of MMP-2, MMP-9, TIMP-1 and TIMP-2 was revealed [26]. Production of MMPs by keratinocytes in acne is also involved in hyperkeratinization of the pilosebaceous unit [27].

Genetic profiling and inflammation
inflammatory cytokines is observed (especially IL-17), which will lead to neutrophils involvement in the pathogenesis of the disease [1,2,4,32]. No circulating autoantibodies nor auto-reactive T lymphocytes are involved [1].
The genetic abnormalities of the γ-synthetase complex are involved in epidermal, follicular and cyst defects [33]. Since 1985 Fitzsimmons suggested a possible autosomal dominant transmission in hidradenitis suppurativa, supported by the identification of positive history in first degree relatives of patients in 34.4% of cases [34]. In 5% of the patients there were identified different mutations of the three genes that produce component proteins of gamma secretase complex: presenilin-1 (PSEN1), presenilin enhancer-2 (encoded by PSENEN), nicastrin (encoded by NCSTN), and anterior pharynx-defective 1 [35]. Reduced gammasecretase activity reduces Notch signaling, involved in normal epidermal and hair follicle differentiation and in modulating T-cell-mediated immune responses [6,36].

Conclusion
Autoinflammatory diseases are entities described relatively recently, being associated with inflammatory neutrophilic infiltrate (including cutaneous infiltration), and genetically characterized.
In the light of these new innate inflammatory disorders, it is a challenge to understand that many of the older diseases can be reclassified into the spectrum of innate immune disorders.
Neutrophilic dermatoses were classified primarily based on clinical and histopathological aspects, while autoinflammatory diseases were genetically and pathophysiologically characterized, so that there may be a degree of overlap that could be difficult to clarify.

Conflicts of Interest
The authors have no conflicts of interest to declare.