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The absorption of saccharin from the rat urinary bladder

of chemical breakdown (D. Gillessen, personal communication). The results obtained with LH-RH agree well with those of other groups which demonstrated similar effects with LH-RH or more potent analogues (Corbin & Beattie 1975; Auclair et a1 1977) after repeated injections. They explained their findings by postulating loss of testicular LH/hCG and prolactin receptors. A marked reduction in testicular weights and circulating testosterone concentrations occurs, possibly in consequence of this effect. The testosterone remaining is nonetheless obviously adequate to prevent atrophy of the accessory sex organs in our experiments. None of the other investigators observed a decrease in circulating FSH concentrations. Whether the reduced FSH concentrations we found are the result of increased negative feedback or reduced hypophysial synthesis is unclear. The new method of peptide administration we describe has the advantage over established techniques of providing a presumably essentially constant low-dose supply of the substance