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Changes of maximum binding capacity and binding power of liver growth hormone receptor in obstructive jaundice rats

Objective To study the changes of the expression and binding power of liver growth hormone receptor(GHR) in obstructive jaundiced rats. Methods A total of 170 Wistar rats were divided into sham operation group and jaundice group with common bile duct obstructed. All animals were observed on day 0, 7 and 14. The maximum binding capacity (B max ) and dissociation constant (k d) of liver GHR were measured with radioligand binding assay of receptors (RBA). The results were studied with the serum levels of total bilirubin, prealbumin and TNF α for correlation analysis. Results The Bmax of liver GHR was significantly lower in obstructive jaundiced rats than sham operation group, which was opposite to the k d changes. The B max values have a positive correlation with serum prealbumin and a negative correlation with serum total bilirubin and TNF α in liver tissue homogenate. But the k d negatively correlated with prealbumin in serum and positively with bilirubin in serum and TNF α in liver tissue homogenate. Conclusion The decrease of B max and binding power of liver GHR might be associated with the higher level of TNF α and other factors and the increased concentration of potentially toxic bile salts in hepatocytes of obstructive jaundiced rats. The reduction of B max and binding power of liver GHR not only decreases the synthesis of liver proteins but also deteriorates the bowel muscous membrane barrier that accelerates the absorption of endotoxin and the release of inflammation cytokines, such as TNF α. Thus, the decreases of B max and binding power of liver GHR play an important role in the pathophysiological alteration of obstructive jaundice.