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[32P incorporation into phospholipids of EHSVi transformed fibroblasts, with regard to adherence to the support].

The rate of 32P incorporation into phospholipids of EHSVi transformed fibroblasts was studied by pulse experiments (2 hours) in cells which are detached from the support by trypsin and either maintained in suspension or replated and in the process of adhering. This is performed comparatively to cells in monolayers. Cells are taken during or after the log phase. Both global incorporation into phospholipids and, in the case of cells in log phase, percentage of incorporation into phosphatidylinositol are lowered, after detachment from the support, in suspended cells. They are stimulated by a new contact with the support. In the same conditions, percentages of incorporation into phosphatidylethanolamine and phosphatidylcholine remain at values they have in cells in monolayers. These values are characteristic of the physiological state of cells in monolayers, in relation with medium factors exhaustion.