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Biosynthesis of Silver Nanoparticles through Biomass of Fungus Aspergillusniger and their Antibacterial Potential

Extracellular biosynthesis of silver nanoparticles by fungus Aspergillus niger which was isolated from
waste water is being reported in the present paper. The production of silver nanoparticles was evidenced by
UV-Vis spectrum, showing the absorbance between 260 to 400 nm. The nanoparticles characterized by
Scanning Electron Microscopy exhibited silver nanoparticles with diameter of 25nm to 75nm. Energy
Dispersive X-ray analysis reveals strong signals in the silver region and confirms the formation of the silver
nanoparticles. The Fourier Transform Infrared Spectroscopy study confirmed that the A. niger mycomass has
the ability to perform both reduction and capping functions on the silver nanoparticles. Compound Microscopy
confirms the self-assembling property of silver nanoparticles. The silver nanoparticles showed remarkable
antibacterial activity against Lactobacillus plantarum, Lactobacillus delbrueckii and Bacillus subtilis
bacterial strains. Reduction of silver ions is an extracellular and rapid process; this information may lead to
the development of easy protocols for biosynthesis of the silver nanoparticles. Antibacterial activity of silver
nanoparticles is important for the development of effective antibacterial agent against those bacteria who are
showing resistance against antibiotic drugs which are available in market.