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[An efficient method for establishing murine tumor cell lines].

Tumor cells were cultured in RPMI1640 medium with 16% fetal calf serum and 5% conditioned medium of PHA-stimulated murine spleen cells. After cloning, the tumor cells were reinoculated into normal susceptible mouse. When tumor developed, they grew easily in subsequent cultures in vitro, thus establishing tumor cell lines. By this method, 5 leukemia cell lines and 2 tumor cell lines have been established.