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Detection of ampicillin contamination in nitrofurantoin preparations by high pressure liquid chromatography.

Abstract: Ampicillin as a contaminant in nitrofurantoin capsules and tablets was determined by high pressure liquid chromatography. The method uses a strong anion-exchange column, 0.01 M KH 2 PO 4 buffer, and an ampicillin working standard as reference. Identity of ampicillin was verified by assaying spiked samples of nitrofurantoin. The method is quantitative over the range 1–10 mcg. of ampicillin.