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A Simple Routine Radioisotopic Method for the Estimatin of Serum Vitamin B12 Using DEAE Cellulose and Human Serum Binding Agent

Summary. A simplified radioisotope method for serum vitamin B12 assay has been described using pooled human serum as a binding agent and equilibrated DEAE cellulose as a separating agent. The normal range found was 185‐1020 pg/ml and a probable significant difference between males and females was discovered. There was no overlap between the normal range and the pernicious anaemia range (30–160 pg/ml). Comparison of the method with microbiological assays (Euglena and Lactobacillus leichmannii) showed good correlation although minor variations between the methods were found and are discussed. The method was precise, economical with reagents and sera, and could easily be introduced as a new procedure in any routine laboratory.