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A MODIFIED METHOD FOR EXTRACTION AND IDENTIFICATION OF INDOLE-3-ACETIC ACID (IAA), GIBBERELLIC ACID (GA 3 ), ABSCISIC ACID (ABA) AND ZEATIN PRODUCED BY PHANEROCHAETE CHRYSOSPORIUM ME446

Besides higher plants, there is evidence that fungi synthesize IAA, GA3, ABA and zeatin as well. According to our findings, Phanerochaete chrysosporium ME446 synthesized the growth substances IAA, GA3, ABA and zeatin as primary and secondary metabolites. Recovery of IAA, GA3, ABA and zeatin were respectively 55.5±10%, 74.6±8%, 51.6±10% and 56.63±6%. We have demonstrated that one can use the same extraction method to determine the different growth substances at the same time, thus this method will be primary and the only method that is applied in this research area.