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Skeletal muscle satellite cell content following acute resistance exercise with or without essential amino acid ingestion in young adults

Recent research shows that the number of Pax7 positive satellite cells increases between 24 to 48 hours following a bout of resistance exercise (RE). However, the behavior of Pax7 positive satellite cells during early postexercise recovery or with essential amino acid (EAA) ingestion has not been determined. Therefore, our aim was to determine whether the number of Pax7 positive satellite cells increase within 6 hours post RE and if this response is potentiated with EAA ingestion. Young (age=27±2 yrs) subjects were randomized into 2 groups consisting of either leg RE (n=5) or leg RE plus ingestion of 20 g of EAA 1 hour following exercise (n=4). Muscle biopsies were obtained from the vastus lateralis at baseline and at 6 hours post‐exercise. Immunohistochemical analysis of Pax7 was assessed via epifluorescent microscopy. We observed no change in the number of Pax7 positive cells from baseline (0.0705±0.012 cells/fiber) to 6 hours post RE (0.0684±0.016 cells/fiber; p = 0.92). Additionally, no change in Pax7 positive cells was noted 6 hours post RE with EAA ingestion (0.0601±0.018 cells/fiber) from baseline (p=0.74). We conclude that an increase in satellite cell content is not detectable during early postexercise recovery (i.e., within 6 hours following leg RE) with or without the ingestion of essential amino acids. Supported by NIH/NIAMS R01 AR049877, NIA P30AG024832, NIH 1UL1RR029876‐01.