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TIRF microscopy analysis of the mechanism of insulin exocytosis.

INSULIN is synthesized, stored, and released from the pancreatic β cells in islets of Langerhans. Insulin pro-motes glucose uptake by tissues such as fat and mus-cle, while simultaneously inhibiting glucose output by the liver. As a result, rising insulin levels depress glucose concentration in the blood. Insulin levels are es-sentially determined by secretory rates because insulin is cleared from the circulation with a t1/2 of less than 10 min. Thus, blood glucose levels in the body are de-pendent on insulin secretion, which varies from minute to minute.