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C‐terminal mechano‐growth factor induces heme oxygenase‐1‐mediated neuroprotection of SH‐SY5Y cells via the protein kinase Ce/Nrf2 pathway

(Original article DOI:10.1002/jnr.22543) Published online 12 April 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI: 10.1002/jnr.22642 Fig. 2. Heme oxygenase-1 knockdown blocks MGF24 neuroprotection of SH-SY5Y cells, A: Cells were transfected with nonsense siRNA to HO-1 (NS HO-1) or antisense siRNA (AS HO-1) for 36 h prior to addition of MGF24 followed 24 h later by HO-1 protein Immunoblotting. HO-1 knockdown effectively blocked MGF24 induction of HO-1, whereas exposure to control NS HO-1 siRNA had no effect. This observation, together with results obtained with RNA/protein synthesis inhibition (see Fig. 1C) supports the notion that MGF24 increases HO-1 levels by gene induction. Data are given as mean 6 S.E.M. *P < 0.005 compared to control, **P < 0.05 compared to control. B: Cells were exposed to siRNA for 36 h, then MGF24 for 24 h, then 6-OHDA for 24 h prior to the MTT assay of viability. HO-1 knockdown by antisense siRNA blocked MGF24-induced cell protection, whereas nonsense siRNA did not block MGF24 protection. Data are given as mean 6 S.E.M. *P < 0.05 compared to control. All experiments were repeated at least three times, and representative results are shown. Journal of Neuroscience Research 89:1142 (2011)