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Digital inline holography of biological specimens

Digital Holography is the technique of numerically reconstructing a three-dimensional (3D) image containing both amplitude and phase information from a two dimensional (2D) interference pattern recorded by the CCD. In this paper, we study the effects of varying the coherence length by using light from two types of source's (1) coherent laser light and (2) spatially filtered incoherent light from a Light Emitting Diode (LED). We present results using both calibrated test objects and biological samples with view to developing a 3D object recognition and classification system.