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Direct 1H NMR determination of the enantiomeric excess of carnitine.

The enantiomeric purity of carnitine may be determined without any derivatisation of the molecule. Preparations of aqueous mixtures containing stereochemically pure L-malic acid, carnitine and europium trichloride lead to the formation of a complex in which the trimethylammonium groups of D- and L-carnitine are diastereotopic. The resolution is observed only when strict experimental conditions are maintained.