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Endophyte‐infected tall fescue diet alters gene expression in heifer luteal tissue as revealed by interspecies microarray analysis

Cattle consuming endophyte‐infected tall fescue grass have an associated reduction in circulating progesterone and reduced reproductive rates. In this study, commercially available rat microarrays were used to analyze the gene expression in luteal tissues from heifers fed endophyte‐free fescue, endophyte‐infected fescue, or endophyte‐infected fescue supplemented with the dopamine (DA) antagonist, domperidone. The number of hybridized spots represented approximately 40% of the total 10,000 rat genes/ESTs evaluated. Each luteal sample was analyzed in triplicate, resulting in within treatment correlation coefficients of ≥0.98. Median values of mRNA abundance from luteal tissue taken from the endophyte‐infected fed heifers revealed 598 genes and ESTs that were down regulated and 56 genes and ESTs that were upregulated compared with luteal mRNA values from the endophyte‐free treatment. There were fewer comparative differences between median values from luteal mRNA from the endophyte‐free versus feeding endophyte‐infected plus domperidone treated heifers. Only 19 genes and ESTs were upregulated and two were down‐regulated. Mol. Reprod. Dev. 67:154–161, 2004. © 2004 Wiley‐Liss, Inc.