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Biochemical Studies on the Effect of Maternal Hyperphenyl‐alaninemia on Fetal Brain Maturation of Mice

Abstract The effects of maternal hyperphenylalaninemia during pregnancy on the biochemical maturation of neonatal mice brains were examined, thus establishing the critical concentration of phenylalanine in maternal blood and the critical period of maternal hyperphenylalaninemia during pregnancy. Hyperphenylalaninemia was induced by giving chow supplemented with 3%, 4%, 5%, or 6% phenylalanine (Phe.) and 0.12% p‐chlorophenylalanine (PCPA) for at least one month and then throughout pregnancy. Some of the pregnant mice fed the 6% Phe. diet before pregnancy received a normal diet after conception (6%: A).