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Cell Free Synthesis Of Ö-Succinylbenzoic Acid In Protein Extracts From Anthraquinone And Phylloquinone (Vitamin K1) Producing Plant Cell Suspension Cultures. Occurrence Of Intermediates Between Isochorismic And o-Succinylbenzoic Acid

Cell free protein extracts from heterotrophic anthraquinone (“Rubiatype”) producing cell suspension cultures of three different Galium species and from a photoautotrophic phylloquinone (vitamin K ,) producing cell suspension culture of Morinda lucida Benth. (Rubiaceae) catalyzed the synthesis of o-succinylbenzoic acid (OSB) from isochorismic acid and a-oxoglutaric acid in the presence of thiamine diphosphate and Mn2+. At least two intermediates in the conversion of isochorismic to o-succinylbenzoic acid were detectable. One of these intermediates is likely to be identical to 2-succinyl-6-hydroxy-2,4- cyclohexadiene-l-carboxylate (SHCHC), a metabolite known to occur in bacterial mutants (menD+) blocked in o-succinylbenzoic acid synthesis. The structure of the second intermediate is as yet unknown. A third intermediate may also occur