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Kinetics of β‐Lactoglobulin Adsorption onto Stainless Steel Surfaces

Adsorption kinetics of β‐lactoglobulin onto stainless steel at low concentrations were studied to determine adsorption rate constants for monolayer coverage. Experimental data obtained from a batch reactor were described quantitatively using a reversible adsorption model which becomes the Langmuir‐type adsorption isotherm at equilibrium. The forward rate constant, k1, and backward rate constant, k2, were 105 mL g−1 min−1 and 5 × 10−3 min−1, respectively. A high‐performance liquid chromatographic system using a stainless steel column packed with stainless steel particles (column adsorption reactor) was also used to measure adsorption with a step change of β‐lactoglobulin solution. The difference in behavior of an adsorption and a desorption isotherm in this column adsorption reactor suggested surface denaturation of β‐lactoglobulin after adsorption which was consistent with other research work. β‐lactoglobulin adsorption in the column adsorption reactor was affected by volumetric flow rate and concentration up to 4 mL min−1 and 100 μg mL−1, respectively.