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Neuropeptide Y concentration within sympathetic nerve endings of skeletal muscle arterioles

The purpose of the study was to determine neuropeptide Y (NPY) concentration within sympathetic nerve endings of gastrocnemius 1A arterioles. Arteriole segments were dissected from the gastrocnemius muscle of male and female F‐344 rats. The 2mm segments were then placed in 400 μl of Krebs‐Ringer buffer and pulverized with a pestle for three minutes. The samples were centrifuged for 15 minutes to remove vascular debris. A competitive peptide enzyme immunoassay (EIA) was used for NPY quantification. Analysis revealed .56±.04 ng/ml and .49 ng/ml in male and female gastrocnemius 1A arterioles, respectively. The intra‐assay coefficient of variation was 5%. These data suggest that NPY levels within gastrocnemius 1A arterioles are substantive in concentration to quantify using this competitive peptide EIA. This research was supported by the Arkansas Biosciences Institute and the National Institute on Aging.