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Candidate human papillomavirus (HPV) type 27b: Nucleotide sequence and heterogeneity with HPV 27

More than 100 human papillomavirus (HPV) types have been reported as tumorigenic agents. HPV types 2 and 27 were identified in benign tumors and classified closely according to the sequence homology. An HPV type 2 related sequence was identified previously in an oral papilloma. However, the correct typing and sequencing was impossible at that time. A candidate HPV was cloned by the long‐PCR technique from an oral papilloma and characterized by nucleotide sequence. This virus was basically HPV type 27, but there was some heterogeneity in comparison with the prototype. Most differences were nucleotide‐sequence variations. Two of four‐nucleotide insertions were frame shift, resulting in longer L1 protein than that of HPV 27. J. Med. Virol. 77:113–115, 2005. © 2005 Wiley‐Liss, Inc.