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Supplementary Figure Legends

Fig. S2. Effect of siRNA-mediated knockdowns on protein levels. (A,B) The stability and detergent-extractability of OST subunits was analysed by direct solubilisation of siRNA-treated HeLa cells in SDS sample buffer followed by infrared immunoblotting. (C) Quantitative representation of the data presented in A,B. Fluorescent signals were quantified, normalised to the corresponding α-tubulin signals, and expressed as a percentage of the signal in control cells.