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Removal of contaminating polymorphonuclear leukocytes to prepare purified lymphocytes for cytotoxicity testing.

Human polymorphonuclear leukocytes (PMN) die, apparently nonspecifically, in the standard tissue typing assay. When the cell preparation contains more than 20% PMN, proper evaluation of the histocompatibility assay is difficult. PMN were noted to aggregate when normal rabbit serum was added as the complement source. Based on the latter observation, a simple technique was developed which reduces the PMN contamination in the suspension of test cells and returns high yields of lymphocytes. Removal of the PMN lowers the high background of dead cells and permits precise evaluation of the microcytotoxicity test results.