{'fraction_non_alphanumeric': 0.024117140396210164, 'fraction_numerical': 0.03359173126614987, 'mean_word_length': 5.916666666666667, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
GCR1‐Dependent Transcriptional Activation of Yeast Retrotransposon Ty2‐917

Transcription of Saccharomyces cerevisiae Ty2‐917 retrotransposon depends on regulatory elements both upstream and downstream of the transcription initiation site. An upstream activation sequence (UAS) and a downstream enhancer stimulate transcription synergistically. Here we show that activation by both of these sites depends on the GCR1 product, a transcription factor which also regulates the genes encoding yeast glycolytic enzymes. Eliminating GCR1 causes a 100‐fold decrease in transcription of Ty2‐917. Activation by the isolated Ty2‐917 UAS also strongly depends on GCR1. Unexpectedly, GCR1‐dependent activation by the Ty2‐917 enhancer is strongly position‐dependent. Activation by the enhancer in its normal position within the transcription unit depended strongly on GCR1, but eliminating GCR1 reduced activation only three‐fold when the enhancer was moved upstream of the transcribed region. Gel mobility shift and DNaseI protection assays indicated that GCR1 binds specifically to multiple sites within the Ty2‐917 UAS and enhancer regions. © 1997 John Wiley & Sons, Ltd.