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Measurement of Total Serum Protein by Near-Infrared Reflectance Spectroscopy

This study describes the measurement of total protein in serum by near-infrared reflectance spectroscopy. With an algorithm, generated by the calibration procedure, the protein content of serum samples was calculated from absorbance data at various wavelengths in the near-infrared. A good correlation (r = 0.993) was found between near-infrared reflectance spectroscopy measurement of serum protein and analysis by the biuret reaction.