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Characterization of the hepatic stellate cell populations in carbon tetrachloride liver rat through Golgi‐Cox Method.

Golgi‐Cox method was used to characterize the hepatic stellate cell (HSC) populations in CCl4 livers. In wistar rats, damage liver was induced by CCl4 administration during two months. After, rats were sacrifiezed and liver tissues were impregnated with the Golgi‐Cox stain, processed and analyzed by Sholl method under light microscopy adapted to a camera lucida. Location, soma cell size, and cytoplasmic processes from HSCs were analyzed. Damage tissue was evaluated with METAVIR score. HSCs were identified for their classical stellate form, and their cytoplasmic processes, making and extense network of reticular fibers. In accord with their soma cell size and location into the hepatic lobule, we identified periportal, perivenular and perisinusoidal hepatic stellate cells. Periportal and perivenular had the larger soma cells, whereas perisinusoidal had the smaller. The morphological changes of the HSCs in CCl4 livers consisted in an increase in the soma cell size, cut and frayly appearance of emerging cytoplasmic processes, and a significant decrease in the number of HSCs. All changes were less pronunciated in tissues with METAVIR score 1 and 2, particularly in the perisinusoidal hepatic stelate cells. HSC populations suffer morphological changes in CCl4 livers, however perisinusoidall cells are the last affected, suggesting that their preservation could be related to the regeneration liver.