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The Role of Polyhexamethylene Biguanide and Silver Nanoparticle Interaction in the Fabrication of Novel Antibacterial Bio-Fibers Using Silk Wastage

Regenerated silk fibers were fabricated through dry-wet spinning process using N-methyl morpholine N-oxide and methanol as solvent and coagulant, respectively. Several concentrations of polyhexamethylene biguanide (PHMB) (0.5, 0.75 and 1% (v/v)) and silver nanoparticles (AgNPs) (5, 15, 55, 95 and 135 ppm) were used as antibacterial agents. Antibacterial property of bio-fibers produced by either master batch or dipping process methods was compared to each other against a gram-positive bacterium, Staphylococcus aurous. Master batch process was indicated the dependency of antibacterial effect on the concentration of antibacterial agents as well as particle size. Maximum bactericidal activity in this process was obtained at concentrations of 55 ppm of AgNPs and 1% (v/v) of PHMB. The result from dipping process showed that various concentrations of PHMB can greatly influence on the results; the interaction of 55 ppm AgNPs and 0.75% of PHMB offered good inhibition effect with the least amount of color change on bio-fibers.