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Genotypes of drug resistant Mycobacterium tuberculosis associated with pulmonary amoebiasis

In this study, 30 Tuberculosis patients with pulmonary amoebiasis who attended Baghdad teaching hospital in Baghdad , Iraq were enrolled during the period from 1st March to 1st November 2019. The sequencing of DNA in (20) M. tuberculosis clinical isolate from patients was used to analyze the mutations of 81-bp rifampin resistance determining region (RRDR) and mutation of V176F located at the beginning of the ropB gene. In (12) resistant isolates, (14) mutation of 22 distinct types, 21 point mutation & 1 insertion have been detected. The most common mutation was found in the codon as follows 507(20%), 526(40%) and 516(4%), while no mutation was found in (10%) 511, 513, 516, 518 and 522 of resistant isolates. All the patients had pulmonary amoebiasis.