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Aggregation-Induced Fluorescence of Carbazole and o-Carborane Based Organic Fluorophore

Carbazole based fluorophores 9-butyl-3,6-bis-(phenylethynyl)-9H-carbazole (1) and 9-butyl-3,6-bis-(2-phenyl-o-carborane)-9H-carbazole (2) were synthesized via Sonogashira type cross-coupling reaction and followed by insertion with decaborane. Compound 1 exhibited far more intense fluorescence than 2 in THF solution, while in solid state 2 exerted stronger fluorescence than 1. The fluorescence quenching behavior of 2 in THF solution could be attributed to the intramolecular charge transfer of donor-acceptor system in 2, which was confirmed by electrochemical experiments and DFT calculations. The fluorescence enhancement of 2 in solid state can be ascribed to aggregational induced packing which was evidenced by crystallographic study. In addition, compound 2 showed typical aggregation induced emission (AIE) behavior.


INTRODUCTION
Organic π-conjugated compounds have attracted much attention in the realm of functional material science for over a few decades because of their intrinsic flexibility on structural designing and functional modifying, as well as low cost and sustainable manufacturing potential in contrast to metal (especially rare-earth metal) based materials (Metzger, 1999;Beverina and Pagani, 2014). Among the organic functional π-conjugated materials, push-pull type chromophores with electron donating (donor) and accepting (acceptor) functional groups are of growing interests for their promising electro-optical properties and straightforward designing strategy (Kivala and Diederich, 2009). Introducing strong donor and acceptor into π-conjugated molecule structure results in complicated photo-and electronic-behaviors, leading to the discovery of various functional materials which could be applied in the field of organic light-emitting diodes (OLED) (Grimsdale et al., 2009;Xiao et al., 2011), semiconducting materials (Zhang et al., 2017), and organic solar cells (Gunes et al., 2007;Cheng et al., 2009;Mishra and Bauerle, 2012).
Researches on AIE have thrived since its discovery in 2001 (Luo et al., 2001), because this type of compounds exert excellent photoelectronic behaviors in condensed or solid state rather than solution, make it possible and convenient to design novel organic photoelectronic devices such as chemo-or bio-sensor, OLED, organic field-effect transistor (Hong et al., 2009(Hong et al., , 2011Ding et al., 2013;He et al., 2015). Herein, we report a carbazole and carborane based organic fluorophore which exhibits typical AIE behavior.

FT-IR and UV-vis Spectra
In the FT-IR spectrum of 1 (Figure 1), the absorption at 2,213 cm −1 is considered to be the C-C triple bond absorption, which disappeared in the FT-IR spectrum of 2, indicating the insertion reaction of decaborane and alkyne moiety of 1. Also, the appearance of strong absorption of B-H vibration at 2,576 cm −1 confirmed the existence of B-H bond in compound 2, indicating the conversion from 1 to 2 (Yin et al., 2018).
The UV-vis absorption spectra of 1 and 2 were recorded in THF solution. As shown in Figure 2, the absorption band at 260, 310, and 330 nm could be assigned to the n-π and nπ * transition of electron-enriched carbazole (Cz) moiety to the phenylacetylene fragment of 1 (Li et al., 2012;Gong et al., 2014), while blue shift was observed for the absorption band at 260 and 310 nm of compound 2. This blue shift effect could be attributed to the wider HOMO/LUMO energy gap on Cz fragment caused by the highly electron-deficient o-carborane (Cb) moiety of 2 (Yokoyama et al., 2018). Moreover, the formation of Cb cluster in 2 leads to the disruption of donor-linker-acceptor conjugated system (Cz-phenylacetylene), hence the absorption band of intramolecular charge transfer transition at 330 nm is FIGURE 1 | FT-IR spectra of (A) compound 1 and (B) compound 2 in 2,100-3,000 cm −1 .

Fluorescence Behaviors
The fluorescence spectra of compound 1 and 2 in various of solutions were recorded, and THF was chosen as solvent for the investigation of the solution excited behaviors. Upon excitation at 304 nm, compound 1 exhibits intense fluorescent emission at around 395 nm in THF solution, and the stokes shifts, which are determined by the difference in the wavelength between position of the band maxima of the excitation and emission spectra of the same electronic transition were almost identical in different solvents ( Figure 3A and Figure S8). In the case of compound 2, significant fluorescence quenching effect was observed ( Figure 3C). Also, in various solutions of 2 at higher concentration, the fluorescence emissions vary from 299 to 360 nm upon excitation at 255 nm ( Figure 3B and Figure S9), indicating the different photoluminescence mechanism. The intense blue fluorescent emission of compound 1 can be assigned to the local excited-state emission of a Cz-phenylacetylene fragment (Li et al., 2019), and the solvatochromic shift of 2 suggested the intramolecular charge-transfer (ICT) behavior between Cb and Cz units (Wee et al., 2012a;Nghia et al., 2018b). The temperature dependency of emission of 2 was also investigated (Figure S10), the results showed that the fluorescence intensity of 2 slightly decreased when temperature rises, implying that the emission band at around 348 nm was from twisted-ICT state, whose emission effect could be affected by environment conditions such as polarity of solvent and temperature . Solid state fluorescence behaviors of compound 1 and 2 are also studied, detailed data are listed in Table 1. As shown in Figure 4, compound 1 exerts fluorescence at 455 nm with a shoulder peak situated at 410 nm, and compound 2 shows a relatively sharp single peak at 550 nm. For compound 1, the major emission band at 455 nm could be assigned to the nπ and π-π * local excited state emission of Cz-phenylacetylene fragment, and the minor shoulder emission peak at 410 nm comes from the isolated emission of Cz moiety (Li et al., 2019). Time-resolved measurement (Figures S11, S12; Tables S2, S3) also confirmed the bi-exponential decay behavior of 1, indicating the double excited state. It is interesting that 2 exhibits stronger fluorescent emission than 1 in solid state, whereas in THF solution the fluorescent emission of 2 is nearly quenched completely. This phenomenon could be related to the photoluminescence mechanism for compound 1 and 2. As we formerly discussed, in THF solution the fluorescent emission of 1 comes from the local excited-state emission of a Czphenylacetylene conjugated fragment, which no longer exists in 2 because of the introduction of Cb moiety (Nghia et al., 2018a). Also, the ICT quenching effect caused by the highly electrondeficient Cb inhibits the fluorescent emission of 2 (Wu Q. J. et al., 2018). Hence in THF solution, compound 1 exerts a much stronger fluorescence intensity than 2.

X-ray Crystallographic Studies
The fluorescence enhancement of compound 2 in solid state is considered to be affected by the aggregational packing in its solid state. The X-ray crystallographic analysis of 2 indicated that two phenyl groups and one Cz unit adopted a Z-shaped arrangement. The C-C bond lengths of two Cb units are without much distinction (1.736 Å for C17-C18 and 1.733 Å for C19-C20, Figure 5A) due to the rigidity of Cb cluster. Nevertheless, the torsion angles around the carbon atoms of Cb explain their environmental difference in detail. The colored carbon atoms and C-C bonds in Figure 5A are from the Cb-phenyl units (including phenyl unit in Cz), they can also be considered as  carbon atoms linked to phenyl units as well, and their torsion angles shall be 180 • in a theoretically free environment without spatial hinderance. As shown in Table 2, the torsion angles of C17 and C18 are relatively smaller than C19 and C20, suggesting that Cb (C17-C18) is in a more distorted environment than Cb (C19-C20), which also indicates that there is a tightly packing style in solid state of compound 2 ( Figure 5B). In THF solution of 2, the rapid charge transfer from the fluorophore of Cz to the electron-withdrawing units of Cb weakens its fluorescent emission, while in solid state of 2, intramolecular vibration and rotation are greatly restricted due to its tightly packing structure, which blocks the non-radiative pathway of excited state annihilation and opens up the radiative channel, thus its solid state fluorescent emission is enhanced (Zhu et al., 2008).
Moreover, the prolonged fluorescence life of 2 also confirmed this restriction effect.

AIE Studies
In order to further demonstrate the AIE behavior of compound 2, the fluorescent emission spectra in THF/H 2 O solution with different volumetric ratios (fraction of water, or f w in vol.%) were determined, and the results are shown in Figure 6.
Compound 2 virtually showed weak fluorescent emission in either THF solution or THF/H 2 O solution with low H 2 O volume fraction. However, its fluorescent emission was significantly enhanced when f w reaches 70%, and the emission intensity kept increasing as the f w rises at higher levels. When f w reached 95%, bright yellow fluorescence could be observed  directly under commercially available UV lamp upon excitation at 254 nm. This typical AIE behavior could be ascribed to the decrease of solubility of 2 with the gradual addition of H 2 O. In THF solution, the fluorescence emission comes from the ICT emission and it is weak because the ICT quenching effect of the introduced carborane unit. As water fraction increased, the emission wavelength around 538 nm originating from the lowest energy ICT excited state was significantly enhanced with the formation of aggregation, which leads to the restriction of vibration and/or rotation of Cb and phenyl unit in compound 2. In the THF solution, the Cb and phenyl unit can rotate, resulting in the dissipation of the excitation energy as thermal energy. However, when the molecules aggregated, the rotation of the peripheral carborane and phenyl units are effectively impeded, which could not lead to non-radiative decay any longer, thus the molecules of 2 become strongly emissive (Furue et al., 2016). In fact, the THF/H 2 O solutions of 2 showed rather poor clarity at higher f w level in contrast to its lower f w solution, indicating that other than precipitation, this aggregation process was conducted at nanoscale (Liu et al., 2011).

Electrochemical Behavior
Furthermore, cyclic voltammograms (CV) were also collected for both 1 and 2 by using dichloromethane with tetrabutylammonium perchlorate (TBAF), as reported in the literature for illustration on their electrochemical properties (Hosoi et al., 2011). The CV curve of 1 (Figure 7A) shows an oxidation peak at 0.82 V and a reduction peak at −0.91 V, might be ascribed to the electrochemical redox process on nitrogen atom in Cz skeleton (Kundu et al., 2003;Naito et al., 2018). In the case of 2 (Figure 7B), one more reduction peak of Cbs was recorded at −1.11 V,  and redox peaks of Cz shifted to higher voltage (from 0.82/−0.91 to 0.93/−0.60), this is supposed to be the electron withdrawing effect of Cbs, making the oxidation process harder by dispersing the electron density on Cz skeleton, and confirming the donor-acceptor behavior in the structure of 2.

DFT Calculation
DFT calculations were performed to determine the HOMO and LUMO energy levels of compound 1 and 2. The Results show that the introduction of o-Cb units lowered both the HOMO and LUMO energy level (from −1.17 to −1.28 eV for LUMO, and from −5.08 to −5.72 eV for HOMO), this could be attributed to the electron-withdrawing ability of the o-Cb groups (Kokado and Chujo, 2009;Kokado et al., 2010;Wee et al., 2012bWee et al., , 2013Guo et al., 2015;Wu X. Y. et al., 2018). From Figure 8, it could be noted that the HOMO and LUMO were mainly located in the Cz-phenylethynyl conjugated skeleton for 1, which is in accordance with the local excited state fluorescence emission. As for the case of 2, the LUMO distributed over the conjugated Cz-Cb skeleton, while its HOMO density was mainly delocalized in the Cz moiety, indicating that the conjugation was greatly reduced in contrast to 1 due to the donor-acceptor behavior between Cb and Cz, also caused the larger energy gap (from 3.91 to 4.44 eV) between the HOMO and LUMO level (Yin et al., 2018).

CONCLUSION
In summary, two carbazole-based fluorophores have been synthesized, and their photo-and electrochemical behavior has been investigated. In THF solution, carbazole based fluorophore 1 exhibits intense blue fluorescence whereas the fluorescence of carbazole/carborane based fluorophore 2 is nearly quenched completely. However, 2 exerts much stronger fluorescence emission than 1 in solid state, and exhibits aggregational induced emission enhancement behavior. Crystallographic study confirmed the tightly packing style in the condensed state of 2. Moreover, electrochemical experiments and DFT calculations were performed to explain the donor-acceptor behavior of the carbazole based fluorophores.

General Experimental Conditions
All manipulations were performed under nitrogen atmosphere by using standard Schlenk techniques unless otherwise indicated.
Chemical reagents except solvents were purchased via commercial sources and used without further purification. Tetrahydrofuran (THF) and toluene were refluxed on sodiumbenzophenone system and freshly distilled prior to use. Triethylamine (Et 3 N) was refluxed on CaH 2 and freshly distilled prior to use. 1 H NMR spectra were recorded on Bruker AVANCE NEO 400 MHz, 11 B, and 13 C NMR spectra were recorded on Bruker AVANCE III HD 600 MHz, and CDCl 3 were used as deuterated reagent unless otherwise specified. FT-IR spectra were recorded on BIO-RAD FTS-40. Solution fluorescence spectra were recorded on PerkinElmer Fluorescence Spectrometer LS55. Solid fluorescence spectra were recorded on Edinburgh Instrument FLS980. 3,6-dibromo-9butylcarbarzole was synthesized according to reported literature (Zhu et al., 2016).

Cyclic Voltammograms Condition
CV tests were carried out with a CHI760B electrochemical workstation in CH 2 Cl 2 containing 0.1 M of sample and 0.1 M of Bu 4 NClO 4 at scan rate of 100 mV/s −1 under a nitrogen atmosphere. A three-electrodes cell system was used, containing a glassy carbon working electrode, a platinum counter electrode and an Ag/AgCl reference electrode, respectively.

Density Functional Calculations
The frontier molecular orbitals of the compounds were performed with the Gaussian 09W. Structural optimization was performed before the calculation of the HOMO/LUMO energies. The Becke3LYP(B3LYP) functional with the 6-311G(d, p) basis set was used for all calculations (Frisch et al., 2009).

X-ray Structure Determination
X-ray diffraction data was collected on a Burker Smart CCD Apex DUO diffractometer with a graphite mono-chromated CuKα radiation (λ = 1.54184 Å) using the ω-2θ scan mode. The data were corrected for Lorenz and polarization effects. The structure was solved by direct methods and refined on F 2 by full-matrix least-squares methods using SHELXTL-2000. All calculations and molecular graphics were carried out on a computer using the SHELX-2000 program package and Diamond software.

DATA AVAILABILITY STATEMENT
All datasets generated for this study are included in the article/Supplementary Material.

AUTHOR CONTRIBUTIONS
JJ performed the design, synthesis experiments, and collection of the data. J-XK and HL made contributions on DFT calculation. YM, QZ, and JZ made contribution on single crystal data interpretation. XC made substantial contribution on data analysis and manuscript writing.