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A systematic program of quality control in clinical microbiology.

A quality control program in clinical microbiology was instituted. The program was designed to parallel quality control systems in other laboratory disciplines. A stable reference material was provided in the form of either lyophilized or frozen bacteria. Control charts were prepared by dividing positive from negative reactions and then separating the known correct from the incorrect reactions. A total of 128 cultures representing 14 different organisms was used to evaluate 25 media and nine reagents, for a total of 1,275 tests in a 12-month period. In addition, 900 antibiotic sensitivity disks were evaluated. There were 35 errors involving media and reagents and 48 errors in antibiotic sensitivity testing. The system evaluated media, reagents, and biological materials as they were used. It permitted rapid identification of problems and provided an educational tool for identification of less common organisms.