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Use of Specific-ion Electrode for the Determination of Un-ionized Ammonia in Fish Blood

Abstract An accurate and simple direct-injection method for the determination of whole blood ammonia in fish uses an ammonia-specific electrode fitted with a flow-through cap (Orion Research, Inc.). Blood from the dorsal aorta of 2-year-old steelhead trout (Salmo gairdneri) and coho salmon (Oncorhynchus kisutch) was obtained without contact with air and injected directly into the micro-flow-through cap for ammonia determination without prior treatment. To determine whether the results obtained by the Conway diffusion and the direct-injection methods were significantly different, we measured whole blood samples from steelhead trout simultaneously; the Conway method gave values 42% higher than the direct-injection method and a Student's t-test verified that ammonia levels were significantly higher (P<0.005) by the Conway diffusion method. The difference was believed to be due to the base hydrolysis of labile nitrogen-containing compounds in fish blood. Test comparison of the direct-injection method and a pl...