{'fraction_non_alphanumeric': 0.030518284661931072, 'fraction_numerical': 0.022757169166008943, 'mean_word_length': 5.272689768976898, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Ultra-high voltage electron microscopy of primitive algae illuminates 3D ultrastructures of the first photosynthetic eukaryote

A heterotrophic organism 1–2 billion years ago enslaved a cyanobacterium to become the first photosynthetic eukaryote, and has diverged globally. The primary phototrophs, glaucophytes, are thought to retain ancestral features of the first photosynthetic eukaryote, but examining the protoplast ultrastructure has previously been problematic in the coccoid glaucophyte Glaucocystis due to its thick cell wall. Here, we examined the three-dimensional (3D) ultrastructure in two divergent species of Glaucocystis using ultra-high voltage electron microscopy. Three-dimensional modelling of Glaucocystis cells using electron tomography clearly showed that numerous, leaflet-like flattened vesicles are distributed throughout the protoplast periphery just underneath a single-layered plasma membrane. This 3D feature is essentially identical to that of another glaucophyte genus Cyanophora, as well as the secondary phototrophs in Alveolata. Thus, the common ancestor of glaucophytes and/or the first photosynthetic eukaryote may have shown similar 3D structures.

the first photosynthetic eukaryote may have been a Cyanophora-like flagellate. As supported by ultrathin section transmission electron microscopy (TEM) and freeze-fracture TEM [20][21][22] , field emission scanning electron microscopy (FE-SEM) recently showed that the whole peripheral surface of naked vegetative cells in several species of Cyanophora is ornamented with angular fenestrations formed by ridges structured by overlapping, leaflet-like flattened vesicles underneath the plasma membrane 21,22 . However, this leaflet-like 3D morphology of the flattened vesicles has not been unambiguously demonstrated in other glaucophyte genera, possibly because FE-SEM cannot reveal surface ultrastructures of the periphery of the protoplast that is enclosed by a cell wall or extracellular matrix in these genera 7 . Although freeze-fracture TEM revealed leaflet-like surface appearances of flattened vesicles in the coccoid glaucophyte genus Glaucocystis 23,24 , the 3D ultrastructural features of the Glaucocystis protoplast periphery are unclear, especially regarding the spatial relationship between the plasma membrane and flattened vesicles [23][24][25][26] . Cyanophora represents one of the two divergent clades of glaucophytes; the other clade includes Cyanoptyche, Gloeochaete, and Glaucocystis 27 . Thus, to provide more detailed ancestral features of glaucophyte cells, ultrastructural characterisation of 3D structures of the protoplast periphery in the latter three glaucophyte genera is required.
Recent advancements in ultra-high voltage electron microscopy (UHVEM) have enabled thick-section micrographs in biological samples 28 . Based on 3D UHVEM tomography, the in situ peripheral ultrastructure of protoplasts can be observed, even when enclosed by extracellular structures 29 . However, 3D UHVEM has not previously been applied to algae or protozoa.

Results
Using UHVEM tomography, the 3D ultrastructural features of the plasma membrane and the flattened vesicles at the protoplast periphery of the two Glaucocystis species were visualised with high contrast (Figs 2 and 3 and Supplementary Movies 1-4). In both species, the flattened vesicles were leaflet-like in shape, lacked a plate-like interior structure, and were distributed throughout the entire protoplast periphery just underneath the single-layered plasma membrane (except for the region near basal bodies; see below), but did not completely enclose the protoplast periphery to form small spaces between the vesicles at the protoplast periphery.
However, our comparative 3D-modelling based on the peripheral tomography clearly showed essential differences in the protoplast periphery between the two species (Figs 2 and 3 and Supplementary Movies 2 and 4). We observed various regions of matured vegetative cells by UHVEM and tomography, as well as ultrathin section TEM (Supplementary Fig. 2 and Supplementary Note 1); the peripheral 3D structures were essentially consistent within each species. In "G. geitleri" SAG 229-1 cells ( Fig. 2 and Supplementary Movies 1 and 2), the plasma membrane exhibited bar-like grooves when viewed from the outside (or bar-like ridges when viewed from the inside) ( Fig. 2b-d,g). These grooves were measured to be 500-1,500 nm long, 60-90 nm wide, and 100-150 nm deep; they were arranged almost in parallel at regular intervals of 500-800 nm. The flattened vesicles just below the plasma membrane were 30-70 nm thick and almost ellipsoidal or ovoid in front view (700-2,000 nm long and 300-600 nm wide) with a bar-like invagination in the centre when viewed from the outside (Fig. 2b,c,e). The invagination of the flattened vesicle was measured to be 500-1,500 nm long, 80-110 nm wide, and 100-150 nm deep. Each groove on the plasma membrane was backed almost entirely with the invagination of the flattened vesicle just underneath the plasma membrane; the backing was often associated with microtubules arranged in parallel (Fig. 2f). The flattened vesicles were almost separated from one another at the protoplast periphery of "G. geitleri" SAG 229-1 cells. Many elongated mitochondria were observed below the flattened vesicles at the protoplast periphery (Fig. 2b).
In G. nostochinearum SAG 16.98 cells ( Fig. 3 and Supplementary Movies 3 and 4), the plasma membrane was almost flat in surface view ( Fig. 3b-d,g), lacking the depression or invagination observed in "G. geitleri" SAG 229-1. The flattened vesicles just underneath the plasma membrane neighboured the inner surface of the plasma membrane at regular patterns in G. nostochinearum SAG 16.98 (Fig. 3b,c,e,f). The vesicles were 30-70 nm thick and elongate-cylindrical in front view (1,500-2,000 nm long and 500-1,000 nm wide); they were almost smooth from a surface view (Fig. 3e). Their marginal regions were often slightly overlapped with one another (Fig. 3f).
Although vestigial flagella in Glaucocystis cells have previously been observed by ultrathin section TEM 23-26 , our UHVEM tomography clearly showed the 3D ultrastructure of the protoplast periphery surrounding basal bodies and neighbouring vestigial flagella in "G. geitleri" SAG 229-1 (

Discussion
The present UHVEM tomography study clearly demonstrated that the plasma membrane of "G. geitleri" SAG 229-1 represented a single, continuous sheet with numerous bar-like grooves that were distributed throughout the surface; the grooves were associated with numerous, leaflet-like flattened vesicles just underneath the plasma membrane (Fig. 2). Except for the presence of grooves, these ultrastructural features of the protoplast periphery in "G. geitleri" SAG 229-1 were essentially the same as those of G. nostochinearum SAG 16.98 (Fig. 3), as well as five species of the motile glaucophyte genus Cyanophora 21,22 ; a single plasma membrane is closely associated with numerous, leaflet-like flattened vesicles distributed throughout the periphery just underneath the membrane. Thus, these 3D structures can be considered common ancestral features of the glaucophytes. Even when 3D structures had not been clarified and molecular data were lacking, Kies 26 already considered the peripheral flattened vesicles ("Lakunensystem") as a unifying morphological characteristic of glaucophytes.
In dinophytes and Chromera (Alveolata), similar 3D structures of the plasma membrane and the underlying leaflet-like flattened vesicles or alveolae can be considered based on SEM/FE-SEM and ultrathin section TEM 2,30-32 . In addition, some haptophytes possess flattened-vesicle-like ultrastructures or peripheral endoplasmic reticulum (PER) just beneath the plasma membrane 33 . Thus, fundamentally identical or homologous peripheral ultrastructures may be distributed in separate lineages or different supergoups within corticates or bikonts (corticates plus Excavata, or eukaryotes excluding Amoebozoa and Opisthokonta 2 ) (Fig. 5). On the other hand, no organism in the other two groups of Archaeplastida (Chloroplastida and red algae) and unikonts (composed of opisthokonts and amoebozoans) contains such complicated peripheral ultrastructures. Given that the glaucophytes represent the most ancestral features of Archaeplastida 5,6 , the leaflet-like flattened vesicles in the protoplast periphery in glaucophyte cells may have been retained from the first photosynthetic eukaryote in the Precambrian period or a more ancient ancestor within the bikonts, as suggested by Cavalier-Smith 19 (Fig. 5). In the ancestors of Chloroplastida and red algae, the flattened vesicles may have been lost during evolution.   The flattened vesicles of Cyanophora contain a plate and completely enclose the protoplast by overlapping with one another at the protoplast periphery to form ridges on the cell surface under FE-SEM 21,22 . In contrast, the present study demonstrated that two divergent species of Glaucocystis have flattened vesicles that lack the plate and are more or less separated from one another just underneath the plasma membrane to form spaces between the vesicles at the protoplast periphery. This difference may reflect the presence or absence of a cell wall in these two genera. Since the Cyanophora cells lack cell walls, the function of tightly arranged flattened vesicles with plates may protect the protoplast or facilitate the formation of cell shape characteristics of the species 21 . It is generally believed that the flagellate vegetative cells represent an ancestral form in the photosynthetic eukaryotes or algae 5,34 . Thus, it is possible that during evolutionary processes from the ancestral Cyanophora-like flagellate to the immotile Glaucocystis cell, the flattened vesicles in the protoplast periphery may have lost their plates in exchange for obtaining a wall to protect the cell periphery.
In lacking motile stages during life cycle in cultured material 23 , the vestigial flagella of Glaucocystis vegetative cells can be considered to be a non-functional organ or evolutionary remnant of flagella of the ancient flagellate ancestor. Thus, Glaucocystis may represent early evolutionary stage from flagellate vegetative cells to nonmotile vegetative cells. The present study clearly showed that flattened vesicles are lacking but ovoid-to-spherical vesicles are distributed below the plasma membrane near the basal bodies and vestigial flagella ( Fig. 4 and Supplementary Movies 5 and 6). Given that these two types of vesicles have different functions, the vestigial flagella may have a cryptic function (e. g. sensory organelle as in neural cilia [35][36][37] in communication with the surrounding cytoplasmic periphery that harbours the ovoid-to-spherical vesicles. Based on morphological comparison at the species level, ultrastructural differences were resolved between the two divergent Glaucocystis species in the plasma membrane and the underlying flattened vesicles (Figs 2 and 3 and Supplementary Movies 2 and 4). In "G. geitleri" SAG 229-1, the plasma membrane and the underlying flattened vesicles formed numerous bar-like grooves that were distributed throughout the protoplast surface, and the flattened vesicles were almost separated from one another at the protoplast periphery ( Fig. 2 and Supplementary Movie 2). In contrast, the plasma membrane and vesicles of G. nostochinearum SAG 16.98 were almost smooth or flat, lacking such grooves or invaginations, and the vesicles were often slightly overlapping with one another at the periphery of the protoplast ( Fig. 3 and Supplementary Movie 4). Thus, ultrastructural diversity of the protoplast periphery is apparent within the genus Glaucocystis, in contrast to previous reports 25,38 .

Conclusions
In the present study, the 3D ultrastructural arrangement of the plasma membrane and the underlying leaflet-like flattened vesicles in the coccoid glaucophyte genus Glaucocystis were clearly observed by UHVEM tomography and 3D-modelling using HPF-FS method. Although plates are lacking within the vesicles, the Glaucocystis periphery is essentially identical based on the 3D ultrastructural arrangement as that of motile glaucophyte genus Cyanophora, as well as alveolates, which suggests that such peripheral ultrastructures may represent ancestral features of the first photosynthetic eukaryote, as well as the first corticate, as suggested by Cavalier-Smith 19 . At the species level, the two species of Glaucocystis were clearly distinguished from each other based on our ultrathin section TEM as well as 3D UHVEM tomographic comparison of peripheral ultrastructures just inside the wall. Hence, UHVEM tomography can be used to explore the 3D ultrastructural arrangement of the periphery, even in the presence of a wall or extracellular matrix, and can be used to compare the subcellular ultrastructure or 3D arrangement of organisms. Thus, further 3D UHVEM tomography and 3D-modelling of other strains or species of Glaucocystis, as well as for other bikonts, will unveil the actual diversity and ancestral ultrastructural features of the bikonts.

High-pressure freezing (HPF) and freeze-substitution (FS) fixation.
Since the HPF-FS fixation method is generally expected to be superior to chemical fixation in preserving the integrity of cellular ultrastructure 13,45,46 , this method was performed for TEM and UHVEM as described previously 13,46 with minor modifications. Briefly, cells were harvested directly from the cultures using a pipette and frozen under high pressure using a high-pressure freezing machine (HPM010; Bal-Tec). The samples were placed onto frozen 4% osmium tetroxide anhydrous acetone at liquid-nitrogen temperature and post-fixed in the solution incubated at − 80 °C for 5 days before warming gradually to − 20 °C for 2 h, then to 4 °C for 1 h and finally to room temperature. The samples were washed three times with anhydrous acetone and Scientific RepoRts | 5:14735 | DOi: 10.1038/srep14735 infiltrated with increasing concentrations of Spurr's resin 47 in anhydrous acetone, and finally embedded in Spurr's resin.
Transmission electron microscopy (TEM) and ultra-high-voltage electron microscopy (UHVEM). Ultrathin section TEM was performed as described previously 48 . Prior to UHVEM observation, thick sections (1 or 2 μ m) were cut using an ultramicrotome (Ultracut E, Reichert-Jung) and mounted on formvar-coated copper grids. The thick sections were stained in 10% uranyl acetate in 70% methanol with 150 W microwave for 30 s and then incubated for 20 min. After washing and drying, the sections were stained in lead citrate with 150 W microwave for 30 s and then incubated for 10 min. Colloidal gold particles (20 or 60 nm in diameter) were deposited on both sides of each section, and the samples were observed using UHVEM (H-3000, Hitachi) at an accelerating voltage of 2 MV. Tomographic image series were recorded using a 4096 × 4096 pixel slow scan CCD camera (TVIPS). Single axis tilt series were obtained from ± 60° with 2° increments. Reconstruction of the tomographic and 3D-modelling was performed as described previously 29 .