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Production of Post-infectious Antibody and Serum Protein Changes in Yellowtail after the Outbreak of Pseudotuberculosis

It has been suggested that cultured yellowtail, Seriola quinqueradiata, recovered from pseudotuberculosis are protected from recurrences. But few studies of post-infectious immunity had been reported. In this study, humoral aspects of post-infectious immunity of yellowtail was examined on the production of agglutinating antibody against whole cells of the infectious agent and its extracts after the outbreak of pseudotuberculosis in the fish farm. Also, serum protein changes had been observed at the same circumstance. Peaks of agglutinating antibody activity against Pasteurella piscicida whole cells extracts, crude lipopolysuccaride and crude protein, were observed in the middle of July and the end of October. The second peak was higer than the first one against whole cells. On the contrary, the first peak was higher than the second one against crude lipopolysuccaride. Antibody activity against crude protein was maintained at a lower level and two low peaks were formed. Rising and faling period of water temperature ranging about 23-25°C corresponds to these peaks. Increases in relative percentage of the γ-globulin region protein in yellowtail sera were found in these periods by cellulose acetate electrophoresis. Increases in number of bands and precipitin lines in the period when first peak was formed were observed by polyacrylamide gel disc electrophoresis and agar gel immunoelectrophoresis. Agglutinating antibody activities of yellowtail sera in both peaks were found in the fractions eluted in the void volume of Sephadex G-200 column. Post-infecgtious antibody against P. piscicida is produced twice a year, respectively during summer and fall i n cultured yellowtail sera. It would be inferred from this study that the secondary immune response appears in the later period. It was found however that the property of agglutinating antibody in the later period is the same as that in the earlier.