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An analysis of the activity of some selected hydrolytic enzymes in the rabbit tongue mucosa and in the skin after cryoapplication according to time of freezing.

The aim of the paper was to examine, by histochemical methods, the reaction of selected hydrolytic enzymes in the rabbit tongue mucosa and in the skin after cryoapplication depending on time of freezing. The results of tissue cryoapplication demonstrated the reaction of hydrolases during cryodestruction and regeneration, and the dynamics of inflammatory process. The tongue mucosa was found to be more susceptible to cryodestruction than the skin of the experimental animals. The longer was the time of cryoapplication, the more distinct was cryodestruction picture of the tissues examined. The observations confirmed the theory of some participation of hydrolytic cellular enzymes in cryonecrosis.