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The Non-linear Logarithm Method (NLLM) to adjust the color deviation of fluorescent images

Fluorescence objects can be excited by ultraviolet (UV) light and emit a specific light of longer wavelength in biomedical experiments. However, UV light causes a deviation in the blue violet color of fluorescent images. Therefore, this study presents a color deviation adjustment method to recover the color of fluorescent image to the hue observed under normal white light, while retaining the UV light-excited fluorescent area in the reconstructed image. Based on the Gray World Method, we proposed a non-linear logarithm method (NLLM) to restore the color deviation of fluorescent images by using a yellow filter attached to the front of a digital camera lens in the experiment. Subsequently, the luminance datum of objects can be divided into the red, green, and blue (R/G/B) components which can determine the appropriate intensity of chromatic colors. In general, the datum of fluorescent images transformed into the CIE 1931 color space can be used to evaluate the quality of reconstructed images by the distribution of x-y coordinates. From the experiment, the proposed method NLLM can recover more than 90% color deviation and the reconstructed images can approach to the real color of fluorescent object illuminated by white light.