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Uptake of Vitamin B12 by Phytohaemagglutinin‐Transformed Lymphocytes

Phytohaemagglutinin (PHA)‐transformed lymphocytes have been used as a model cell system to study the uptake of radioactive vitamin B12 by haemopoietic cells. Both mature granulocytes and PHA‐transformed lymphocytes took up more vitamin B12 than mature, non‐transformed lymphocytes. Uptake of vitamin B12 by PHA‐stimulated lymphocytes was greatest at 48–72 hr of culture, i.e. at about the time or just before the time of peak DNA synthesis.