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Antibody response to bacteriophage ϕX 174 in vitro: Demonstration of immunological memory in terms of IgM and IgG responses

The initiation of a primary‐type antibody response to bacteriophage ϕX 174 has been studied in organ cultures of lymph nodes from normal rabbits. Lymph nodes are also removed for culture at various times following a primary injection of ϕX in the rabbits and their responsiveness to a second anti‐genic stimulus investigated. All the cultures are maintained for 2 to 4 weeks and the antibody responses followed throughout. The following points are shown.