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Protein interactome mapping of Porphyromonas gingivalis provides insights into the formation of the PorQ-Z complex of the type IX secretion system.

Porphyromonas gingivalis is an anaerobic gram-negative human oral pathogen highly associated with the more severe forms of periodontal disease. P. gingivalis utilizes the type IX secretion system (T9SS) to transport ∼30 cargo proteins including multiple virulence factors to the cell surface. The T9SS is a multi-protein complex consisting of at least 19 proteins and recently we characterised the protein interactome of these components. Like the T9SS, almost all biological processes are mediated through protein-protein interactions (PPIs). Therefore, mapping PPIs are important to understand the biological functions of many proteins in P. gingivalis. Herein, we provide native migration profiles of over 1000 P. gingivalis proteins. Using the T9SS, we demonstrate that our dataset is a useful resource for identifying novel protein interactions. Using this dataset and further analysis of T9SS P. gingivalis mutants we discover new mechanistic insights into the formation of the PorQ-Z complex of the T9SS. This dataset is a valuable resource for studies of P. gingivalis. This article is protected by copyright. All rights reserved.