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SARS-CoV-2 Spike protein S2 subunit modulates γ-secretase and enhances amyloid-β production in COVID-19 neuropathy

SARS-CoV-2-induced multi-lineage neural cell dysregulation has been documented 1 . SARS-CoV-2 infection elevates neuroin ﬂ ammation 2 , alters brain structure 3 leads to abnormal accumulation of neurodegenerative amyloid- β (A β ) and phosphorylated tau 4,5 , and increases the risk of cognitive impairment 6 in COVID-19 patients. However, the mechanism underlying neurological dys-functions following SARS-CoV-2 infection remains largely unknown.

To evaluate long-term impact of SARS-CoV-2 infection to the brain, the hACE2 transgenic mice as described previously 7 were intranasally infected with a single low dose (1 × 10 2 TCID 50 ) of prototyped SARS-CoV-2 and maintained for up to 30 days post infection (dpi) (Fig. 1a). Presence of SARS-CoV-2 was found in cortex at 7 dpi but not at 30 dpi by the viral Spike protein immunostaining ( Supplementary Fig. S1a). We found a remarkable activation of Iba1 + microglia and GFAP + astrocytes in the hippocampus and cortex of infected mice at 30 dpi (Supplementary Fig. S1b-e), suggesting a persistent neuroinflammation. We looked for further brain changes by analyzing transcriptomics of the hippocampal tissues at 30 dpi ( Supplementary Fig. S2a). A series of dysregulated genes or pathways were identified in response to SARS-CoV-2 infection (Supplementary Table S1). Gene ontology analysis revealed that the upregulated genes were mainly enriched in pathways related to antiviral immune response and aging, while the downregulated genes were enriched in neuronal function-related pathways such as synaptic vesicle clustering (Fig. 1b). Specifically, the neuroinflammatory genes Trem2, Ifitm3 and Gfap were significantly upregulated, whereas the neuronal genes Map2 and Synapsin II (Syn2) were downregulated. Unexpectedly, mRNA levels of amyloid precursor protein (APP) processing-related genes such as Bace1, Aph1, Presenilin 1 (Psen1), Nicastrin (Ncstn), and Psenen were unchanged (Fig. 1c). The upregulation of Trem2 and Gfap, the downregulation of Map2 and Syn2, and the un-alteration of Bace1 and Psen1 were validated by quantitative realtime PCR (Supplementary Fig. S2b). Such expression patterns were also observed in the brain transcriptomic dataset obtained from COVID-19 patients by singlenucleus RNA sequencing 2 ( Supplementary Fig. S3a-c). These results suggest that the presence of the neurodegenerative hallmarks in COVID-19 brain might not be regulated at the transcriptional level but through an unknown regulatory mechanism.
An enzymatic cleavage of the APP by both β-secretase and γ-secretase, acting together, produces Aβ, which can cause widespread neuropathy within brain and is a pathological hallmark of AD 10 . The cleavage site of γsecretase is located on C-terminal APP, namely APP C-terminal 99 fragment (APP-C99) only contains the cleavage site of γ-secretase. As a result, APP intracellular domain (AICD) at C-terminal C99 domain is produced by γ-secretase cleavage 11 . To examine whether the interaction between S-S2 and γ-secretase modulates the cleavage activity, we initially detected the production of AICD. Immunoblot showed that prototyped S-S2 promoted the production of flag-tagged AICD, whereas the expression of APP and NCT was largely unchanged (Fig. 1i). This was validated by the observation of the increased production of flag-tagged AICD in U251-C99 cells while the expression of APP and NCT was largely unaltered (Fig. 1j). Similarly, Omicron S-S2 also increased the production of flag-tagged AICD, while the expression of APP and PS1 was unchanged (Fig. 1k). These results demonstrate that the increased production of AICD from the APP cleavage was caused by S-S2 modulation of γ-secretase.
HEK-APP695 12 cells transfected with prototyped S-S2, but not the M, produced higher level of Aβ40 than nontransfected cells via enzyme-linked immunosorbent assay (ELISA), while a similar increase of Aβ40 was also observed upon the transfection of IFITM3 as a positive control 13 (Supplementary Fig. S6a). To further evaluate this effect, we used neuronal cells including U251 and mouse primary neurons, both endogenously expressing APP protein. Lentiviral transduction of prototyped S-S2 or IFITM3 invariably caused the increase of Aβ40 or Aβ42 production as compared to empty-vector lentivirus transduction in U251 cells (Supplementary Fig. S6b) and mouse primary neurons (Fig. 1l), whereas lentiviral transduction of the M did not have such an effect. As expected, mouse primary neurons transduced with lentiviral Omicron-S-S2 produced higher Aβ40 and Aβ42 levels ( Supplementary Fig. S6c). These results demonstrate (see figure on previous page) Fig. 1 SARS-CoV-2 Spike protein S2 subunit binds to and modulates γ-secretase to enhance Aβ production. a hACE2 transgenic mice were intranasally (i.n.) infected by prototyped SARS-CoV-2. Brain cortical or hippocampal tissues were collected for immunofluorescence (7 or 30 dpi) and RNA-seq analysis (30 dpi). b Enrichment analysis of representative biological processes in the hippocampal RNA-seq data at 30 dpi in a. c Expression pattern of representative genes within the categorized gene ontology as indicated. d-g co-IP assays of anti-flag monoclonal antibody in HEK293T cells transfected with vector, S-S1-flag or S-S2-flag, together with myc-tagged PEN-2 (d), APH-1 (e), PS1 (f), and NCT (g). h co-IP assays of anti-flag monoclonal antibody in HEK293T cells co-transfected with myc-tagged PEN-2, APH-1, PS1 and NCT, together with S-S2-flag. i HEK293T cells were transfected with expression vector of APP-C99 with C-terminal flag tag (0.5 μg) and increasing amount of prototyped S-S2-Flag (0, 0.25, and 0.5 μg) in 12-well plates for 36 h. j U251-C99 cells were transduced with lentivirus carrying prototyped S-S2-Flag in 12-well plates for 36 h. The production of AICD (red arrows) in i and j was examined by immunoblot analysis. k HEK293T cells were co-transfected with expression vector of APP-C99 with C-terminal flag tag (APP-C99-Flag, 0.5 μg) and increasing amount (0, 0.25 or 0.5 μg) of Omicron S-S2-Flag in 12-well plates for 36 h. The production of AICD (red arrow) from APP-C99 was detected by immunoblot analysis. l Mouse primary neurons were isolated from embryonic (E18.5) brains and cultured in 24-well plates. Neurons were transduced with lentivirus carrying empty vector (vector), prototyped S-S2, M, or IFITM3 for 36 h. The Aβ40 (left) and Aβ42 (right) levels in the supernatants were quantified by ELISA. Means ± SD; n = 4; n.s., not significant; *P < 0.05; **P < 0.01, oneway ANOVA with Bonferroni's post hoc test. m Representative anti-Aβ antibody staining of cortical (CTX) and hippocampal (HP) sections in APP/ PS1ΔE9 mice with AAV delivery of prototyped S-S2 (AAV-S-S2) and AAV control (AAV-Vector). Scale bar, 500 μm. n Quantitative analysis of the number of Aβ plaques and the percentage of area covered by Aβ plaques in cortical (upper) and hippocampal (bottom) tissues in m. Each slide was counted for Aβ plaque number and Aβ plaque area via ImageJ software, and the percentage of the plaque area was calculated. o Representative immunofluorescence of microglial marker TMEM119 protein in hippocampal sections of AAV-S-S2 or AAV-Vector. Scale bar, 30 μm. p Quantification of percentage of TMEM119 + area in o. Statistical analyses for n and p, Means ± SD; n = 6 (AAV-Vector group) or n = 7 (AAV-S-S2 group); *P < 0.05; **P < 0.01; ***P < 0.001, Student's t-test.
To investigate whether S-S2 modulates γ-secretase in vivo, we examined hippocampal and cortical tissues of APPswe/PSEN1dE9 (hereafter referred to as APP/ PS1ΔE9) mice, which have mutated human APP (Swedish mutations K595N/M596L) and the human PSEN1/PS1 lacking exon 9 14 , 2 months after AAV delivery of S-S2. Immunohistochemistry showed a widespread overexpression of S-S2 in hippocampal tissues (Supplementary Fig. S7a). Measurement of soluble and insoluble Aβ levels using ELISA showed that soluble Aβ42 species, but not insoluble Aβ40 and Aβ42 and soluble Aβ40, were markedly increased in cortical tissues of APP/PS1ΔE9 mice with S-S2 overexpression relative to empty vector group ( Supplementary Fig. S7b-e). Similarly, immunostaining showed a significant increase of Aβ burden in cortical and hippocampal tissues of APP/PS1ΔE9 mice after S-S2 delivery (Fig. 1m). The delivery of S-S2 increased the Aβ plaque-deposited area in cortical and hippocampal tissues of APP/PS1ΔE9 mice (Fig. 1n). Overall, overexpression of SARS-CoV-2 S-S2 in hippocampus exacerbated Aβ burden in APP/PS1ΔE9 mice.
Neuroinflammation, an important factor in AD pathogenesis, promotes Aβ pathology 15 . A significant increase of Iba1 + microglia and GFAP + astrocytes ( Supplementary  Fig. S8a-c) was observed in hippocampal tissues of APP/ PS1ΔE9 mice after delivery of S-S2. Staining of microglial marker TMEM119 validated the elevated neuroinflammation following S-S2 delivery (Fig. 1o, p). These results demonstrated that S-S2 overexpression increased Aβ deposit and caused neuroinflammation in Aβ pathology of APP/PS1ΔE9 mice. Both the area covered by NeuN-labeled neuronal cells and the thickness of NeuNlabeled CA1 subfield ( Supplementary Fig. S8d-f) were not significantly altered in hippocampal tissues following S-S2 delivery, suggesting that S-S2 overexpression might not cause neuronal loss after AAV delivery for 2 months.
In summary, we have identified S-S2 subunit as a γsecretase modulatory protein and revealed a previously unknown mechanistic insight into COVID-19-related neuropathological sequelae (Supplementary Fig. S9). A systematical examination of multiple Omicron subvariants ( Supplementary Fig. S10) on potential brain dysfunction would be inspired in future studies. The Spike protein could function as an immune switch to increase γsecretase activity and Aβ production and contribute to neurological changes in COVID-19 patients.