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Electrophoretic studies on blood and tissue proteins of normal and regenerating Ampullarius glaucus (Gastropoda).

Changes in blood and soluble tissue proteins of the snail Ampullarius glaucus were studied by acrylamide gel and cellulose acetate electrophoresis in normal snails and those undergoing shell regeneration. Normal blood regularly has five bands (cellulose acetate). A sixth band was present in 19% of normal and 77% of regenerating snails. Two new protein bands appeared in the mantle during regeneration (acrylamide gel), and these corresponded to two bands of the normal hepatopancreas. The hepatopancreas showed no change in the number of protein bands during regeneration. Total blood calcium and total blood protein were not significantly altered during regeneration. The crystal type of both normal and regenerated shell was aragonite.