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Comparing ultraweak bio-chemiluminescence emission in wounded green and etiolated soybean cotyledons

Ultraweak bio-chemiluminescence (UBC) from germinating soybean (Glycine max L. Merr) cotyledon under mechanical wounding was observed by a high sensitivity imaging system based on an intensified charge couple device (ICCD) detector or a high sensitive single photon counter (SPC) device. The UBC imaging showed that the intensity of UBC at the injury location on a wounded green cotyledon was greater than on a wounded etiolated cotyledon. Based on results with a SPC UBC intensity of wounded green cotyledon was high at first and then gradually decreased. The emission spectrum of wounded green cotyledon had a greater proportion of red light. The increase in UBC of wounded etiolated soybean cotyledon was less than that of green ones. The emission spectrum of wounded etiolated cotyledon had a greater amount of orange light. The data suggest that most of the UBC in green cotyledon was due to damaged chloroplasts and mitochondria. Our data suggest that oxidation of damage's tissue lead to the production of ROS. Electronic excitation energy was transfered from the excited molecules by ROS to Chl-a in the thylakoid membranes, so the intensity of UBC in the wounded soybean green cotyledon was obviously higher than in the wounded etiolated soybean cotyledon.