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Are postprandial responses influenced by melatonin production

There is increasing evidence from animal studies that melatonin may have a role in glucose homoeostatasis. Melatonin administration in normal weight rats has been shown to reduce body weight and intra abdominal adiposity. However, the evidence for the role of melatonin on glucose in human subjects is scant although Peschke reported a reduction in melatonin secretion in diabetics and metabolic syndrome suggesting that the melatonin signal is essential for glucose homoeostasis and regulation. This study investigates the postprandial hormone and metabolic responses after a low glycaemic index (LGI) meal consumed in the late evening in the presence or absence of light. Light suppresses melatonin and therefore, in the bright-light condition melatonin levels would be low while in dim conditions (<5 lux) melatonin levels would increase across the evening. It was hypothesised that in bright light conditions, when melatonin levels were low, postprandial insulin levels would be higher than under dark conditions, when melatonin is not suppressed by light. In a randomised control, single-blind crossover study eight healthy subjects (3F, 5M) mean age 21.5 year (1.6 SD) with a BMI 21.4 kg/m were given an LGI meal at 18.00 h followed by further LGI meal at 23.00 h on two separate occasions. On the day of the study subjects had a control breakfast (2585.71 kJ (1 kcal = 4.184 kJ)) at 07.00 h and lunch (3368.12 kJ) at 13.00 h. They arrived in the clinical unit at 18.00 h and were given a LGI meal (2585.71 kJ) followed by an LGI evening meal (2397.43 kJ) at 23.00 h. Blood samples where obtained using the finger prick technique at regular intervals for 2 h starting directly prior to the consuming the evening meal. Actiwatches were worn by all subjects in order to monitor their activity and sleep–wake cycles. Subjects were asked to complete a 48 h urine collection the week prior to the study to assess the timing and levels of their melatonin rhythm using six sulphatoxymelatonin (aMT6), the major urinary metabolite of melatonin. Urine samples were also collected during the study to measure aMT6s levels. Plasma samples were analysed for glucose using the YSI 2300 analyser. TAG and NEFA were analysed using I-LAB 650 using enzymatic kits. Plasma insulin was measured by human specific RIA kit. aMT6 was measured using a competitive RIA. Comparison between total aMT6 production on each occasion was analysed by paired t-test. The postprandial hormone and metabolic responses were analysed by repeated measure ANOVA. P<0.05 was regarded as significant.