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Extraction of water-soluble organic matter from mineral horizons of forest soils

Dissolved organic matter (DOM) is involved in many important biogeochemical processes in soil. As its collection is laborious, very often water-soluble organic matter (WSOM) obtained by extracting organic or mineral soil horizons with a dilute salt solution has been used as a substitute of DOM. We extracted WSOM (measured as water-soluble organic C, WSOC) from seven mineral horizons of three forest soils from North-Rhine Westphalia, Germany, with demineralized H 2 O, 0.01 M Cacl 2 , and 0.5 M K 2 SO 4 . We investigated the quantitative and qualitative effects of the extractants on WSOM and compared it with DOM collected with ceramic suction cups from the same horizons. The amounts of WSOC extracted differed significantly between both the extractants and the horizons. With two exceptions, K 2 SO 4 extracted the largest amounts of WSOC (up to 126 mg C kg -1 ) followed by H 2 O followed by CaCl 2 . The H 2 O extracts revealed by far the highest molar UV absorptivities at 254 nm (up to 5834 L mol -1 cm-1) compared to the salt solutions which is attributed to solubilization of highly aromatic compounds. The amounts of WSOC extracted did not depend on the amounts of Fe and Al oxides as well as on soil organic C and pH. Water-soluble organic matter extracted by K 2 SO 4 bore the largest similarity to DOM due to relatively analogue molar absorptivities. Therefore, we recommend to use this extractant when trying to obtain a substitute for DOM, but as WSOM extraction is a rate-limited process, the suitability of extraction procedures to obtain a surrogate of DOM remains ambiguous.