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Correlation of contrast-enhanced ultrasound with two distinct types of blood vessels for the assessment of angiogenesis in lewis lung carcinoma.

OBJECTIVE
The aim of our study was to evaluate tumor angiogenesis in Lewis lung carcinoma (LLC) of mice using a contrast-enhanced ultrasound (CEUS) examination, and to determine the correlation of contrast-enhanced ultrasonographic parameters with different blood vessel markers of microvessel density (MVD).


MATERIALS AND METHODS
Subcutaneous Lewis lung carcinomas were established in 25 mice, which were evaluated by contrast-enhanced US using SonoVue (a second-generation US contrast agent). The results were recorded as digital video images and the time-intensity curves and hemodynamic parameters were analyzed. Pathological tumor specimens were obtained just after US examination. Tumor specimens were stained with hematoxylin and eosin (H & E) and expression of CD31 and CD34, the different endothelial cell markers, was determined by immunohistochemical straining. Then the relationship between the CEUS parameters and the level of MVD was analyzed.


RESULTS
Two distinct types of microvessels were identified in Lewis lung carcinoma: differentiated (CD34 +) and undifferentiated (CD31 +) vessels. A significant correlation was found between CEUS parameters and undifferentiated MVD (CD31 + vessels) in LLC (P < 0.05). There was a reverse correlation between the different MVDs.


CONCLUSION
The study showed that among the distinct types of vasculature (CD31 + and CD34 +) in Lewis lung carcinoma, the former correlated with the CEUS parameters. Therefore, CEUS using a second-generation US contrast agent may be useful for the evaluation of tumor angiogenesis of LLC of mice.