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Growth Patterns of Jojoba Male and Female Leaf Callus with Various Ammonium and Nitrate Sources

Abstract Male and female leaf discs of Jojoba [Simmondsia chinensis (Link) Schneider] were cultured on Murashige and Skoog (MS) media supplemented with various nitrate:ammonium ratio and phytohormones concentrations. For the optimum callus growth, hormonal concentrations were remained equal for both male and female leaf tissues i.e., 0.4 mg L−1 2,4‐dichlorophenoxyaceticacid, 1.25 mg L−1 6‐benzyladenine and 0.5 mgL−1 kinetin. However, a statistically significant difference was observed when Murashige and Skoog media was supplemented with an additional nitrogen source. In female leaf tissue, maximum fresh and dry weights were recorded in Murashige and Skoog media supplemented with an additional source of NO3 −:NH4 + (60 mM) whereas in male leaf tissue this addition was inhibitory. This study suggests that nitrogen requirement may be different for optimum callus growth in both male and female leaf tissues.