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Diaporthe seed decay of soybean [Glycine max (L.) Merr.] is endemic in the United States, but new fungi are involved.

Diaporthe seed decay can compromise seed quality in soybean [Glycine max (L.) Merr.] in the warm and humid production areas of the United States during crop maturation. In the current study, 45 isolates of Diaporthe were recovered from seed sampled from soybean fields affected by Diaporthe-associated diseases in eight U.S. states in 2017. The isolates obtained belonged to 10 species of Diaporthe based on morphology and phylogenetic analyses of the internal transcribed spacer, the partial translation elongation factor 1-alpha, and beta-tubulin gene sequences. The associated species included D. aspalathi, D. caulivora, D. kongii, D. longicolla, D. sojae, D. ueckerae, D. unshiuensis and three novel fungi, D. bacilloides, D. flavescens and D. insulistroma. One isolate each of the 10 species was examined for pathogenicity on seed of cv. 'Sava' under controlled conditions. Seven days post-inoculation, significant differences in the percentages of decayed seeds and seedling necrosis were observed among the isolates and the non-inoculated control (p<0.0001). While the isolates of D. bacilloides, D. longicolla, and D. ueckerae caused significantly greater percentage of decayed seeds (p<0.0001), the isolate of D. aspalathi caused the greatest seedling necrosis (p<0.0001) when compared to the non-inoculated control. The observation of new fungi causing Diaporthe seed decay suggests the need for a more comprehensive survey in the U.S. soybean producing areas since members of the genus Diaporthe appear to form a complex that causes seed decay.