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NMR of anthocyanins: Assignments and effects of exchanging aromatic protons

Pelargonidin‐3‐O‐β‐glucopyranoside was isolated from an acidified methanolic extract of frozen strawberries, purified by several chromatographical techniques and subjected to homo‐ and heteronuclear proton and carbon NMR analysis, including selective decoupling, DQF‐COSY, NOESY, SEFT, HMQC and HMBC. Several strategies for the unambiguous assignment of the aglycone H‐6 and H‐8 resonances are discussed. Secondary isotope effects and different deuterium exchange rates of the protons were found.