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IMMUNOCHROMATOGRAPHIC TEST SYSTEM FOR SIMULTANEOUS DETECTION OF PHYCOTOXINS MICROCYSTIN-LR AND DOMOIC ACID

An immunochromatographic test system has been developed for the simultaneous detection of two phycotoxins, domoic acid (DA) and microcystin-LR (MC-LR). The assay is implemented in an indirect competitive format using anti-species antibodies labeled with colloidal gold nanoparticles. The instrumental/visual detection limits are 2/80 and 0.05/0.3 ng/mL for DA and MS-LR, respectively; the assay duration is 18 min. The applicability of the developed test system for the determination of phycotoxins in seawater is shown. This work was supported by the Russian Science Foundation (project no. 20-43-07001).