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Cyclic Tensile Strain promotes the ECM Synthesis of Cranial Base Synchondrosis Chondrocytes by upregulating miR-140-5p.

OBJECTIVE
This study aimed to explore the role of miR-140-5p in cranial base synchondrosis chondrocytes (CBSCs) under cyclic tensile strain (CTS).


SETTING AND SAMPLE POPULATION
25 1-week-old Sprague Dawley rats from Shanghai Laboratory Animal Center, Chinese Academy of Sciences were used.


MATERIAL & METHODS
The second passage of CBSCs was applied with CTS at 10% elongation (1 Hz) for 24 hours. MiR-140-5p levels in CBSCs were detected by qRT-PCR. The role of miR-140-5p in CBSCs was evaluated by transfection of mimics and inhibitor. RNA sequencing and online search of miRNA databases (TargetScan, miRDB and miRanda) were used in prediction of miR-140-5p targets. A luciferase reporter assay was applied to identify the target gene of miR-140-5p.


RESULTS
Compared with the control, the expression of Col2a1 and Sox9 was significantly higher after CTS (p<0.05). Also, CBSCs demonstrated higher expression of miR-140-5p after CTS loading for 24 hours (p<0.05). Overexpression of miR-140-5p promoted ECM synthesis under CTS loading environment, while suppression of miR-140-5p inhibited the effect. Bloc1s2 was a putative target gene of miR-140-5p.


CONCLUSIONS
The expression of ECM in CBSCs could be promoted by CTS and miR-140-5p might play a role in this process through targeting Bloc1s2. This article is protected by copyright. All rights reserved.