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Nitrogen metabolism in the ovine stomach: 1. The transfer of urea from the blood to the rumen

SUMMARY The effect of single, intravenous injections of urea on the concentration of ammonia in the rumen has been examined in conscious and anaesthetized sheep. In conscious sheep maintained on a diet providing 11·4 g nitrogen/day, the net increase in concentration of rumen ammonia after the injection of urea showed negative correlations with the pre-injection concentrations of rumen ammonia and of plasma urea. The maximum concentration of ammonia attained in the rumen after the injection wasx 16·6 ± 0·28 mg NH3-N/100 ml. No such correlations were found in conscious sheep given a diet providing 20·7 g N/day. In addition, the maximum concentration of ammonia attained in the rumen after the injection of urea was significantly lower at 10·7 ± 0·91 mg NH3-N/100 ml, and the net increases in concentration were smaller. Starvation for one day reduced the net increment in rumen ammonia concentration after urea injection in animals given the lower-N diet, to the same low level observed in fed or starved sheep maintained on the higher-N diet. Differences were observed in the transfer of blood urea to the rumen in experiments with anaesthetized sheep which had been maintained on the lower-N diet for long or short periods beforehand. Animals which had been left to graze, and then housed indoors and given the lower-N diet for a short period before the experiment, showed much smaller increases in rumen urea plus ammonia concentration after the injection of urea than sheep which had been given the same diet for a long period. There was no difference in the response of the salivary secretions to the urea injections.