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A technetium‐99m red cell survival technique for in vivo compatibility testing

A technique for labeling red cells with Technetium‐99m was developed using in vitro reduction by stannous pyrophosphate. The use of Technetium‐99m and Chromium‐51 enabled simultaneous determination of 60‐minute autologous and heterologous red cell survival using small aliquots. In vivo pretransfusion red cell compatibility in a patient with a panagglutinin was identical to that with 51Cr‐labeled cells. Simultaneous testing of two heterologous donor units or testing multiple units in the course of a few days was also feasible. In nine studies (one patient and six healthy controls) no significant elution of the 99mTc was observed over 1 hour.