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[Protein mapping of normal circulating cells: lymphocytes and monocytes].

Purified lymphocyte and monocyte populations were obtained from peripheral blood cells by ficoll-hypaque gradient and elutriation in different normal individuals. The two types of mononuclear cells were then analyzed by 2-dimensional gel electrophoresis. More than 1,000 spots were detected by our computer system and analyzed for each cell type. Automatic picture comparison showed a typical pattern for the two different populations with excellent reproducibility.