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EFFECT OF PROCESSING VARIABLES IN FORMULATION AND DEVELOPMENT OF BIODEGRADABLE MICROPARTICLES Research Article

The aim of the present work was to investigate the preparation of microspheres as potential drug carriers for proteins, intended for controlled release formulation. The hydrophilic Bovine Serum Albumin (BSA) was chosen as a model protein to be encapsulated within two different grades of poly (D, L-lactide-co-glycolide) (50:50) in three different concentrations of 5, 10, 15 % W/V for each. Microspheres were prepared using a w/o/w double emulsion solvent evaporation method. Different parameters influencing the molecular weight, particle size, entrapment efficiency and in vitro release profiles were investigated. The microspheres prepared with different concentration and hydrophilicity of poly(D,L-lactide-coglycolide) polymers were non porous, smooth surfaced and spherical in structure under scanning electron microscope with a mean particle size ranging from 34.58 to 97.56 µm. The protein loading efficiency determined by Hartree lowrey’s protein detection method was found varied from 40.25 to 71.54 % of the theoretical amount incorporated. The in vitro release profile of bovine serum albumin from microspheres presented two phases, initial burst release phase due to the protein adsorbed on the microsphere surface, followed by slower and continuous release phase corresponding to the protein entrapped in polymer matrix. The release rate was fairly constant after an initial burst release. Consequently, these microspheres can be proposed as controlled release protein delivery system.