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Transcriptome Analyses Reveal Essential Roles of Alternative Splicing Regulation in Heat-Stressed Holstein Cows

Heat stress (HS) severely impacts the productivity and welfare of dairy cows. Investigating the biological mechanisms underlying HS response is crucial for developing effective mitigation and breeding strategies. Therefore, we evaluated the changes in milk yield, physiological indicators, blood biochemical parameters, and alternative splicing (AS) patterns of lactating Holstein cows during thermoneutral (TN, N = 19) and heat-stress (HS, N = 17) conditions. There was a significant (p < 0.05) decline in milk yield as physiological indicators increased after exposure to natural HS conditions. The levels of eight out of 13 biochemical parameters of HS were also significantly altered in the presence of HS (p < 0.05). These results demonstrate that HS negatively influences various biological processes of Holstein cows. Furthermore, we investigated AS events based on the RNA-seq data from blood samples. With HS, five common types of AS events were generally increased by 6.7–38.9%. A total of 3470 AS events corresponding to 3143 unique genes were differentially alternatively spliced (DSGs) (p-adjusted < 0.05) between TN and HS groups. The functional annotation results show that the majority of DSGs are involved in mRNA splicing and spliceosomal complex, followed by enrichment in immune and metabolic processes. Eighty-seven out of 645 differentially expressed genes (DEGs) (fold change ≥ 1.5 and false discovery rate < 0.05) overlapped with DSGs. Further analyses showed that 20 of these genes were significantly enriched for the RNA splicing, RNA binding, and RNA transport. Among them, two genes (RBM25 and LUC7L3) had strong interrelation and co-expression pattern with other genes and were identified as candidate genes potentially associated with HS responses in dairy cows. In summary, AS plays a crucial role in changing the transcriptome diversity of heat-stress-related genes in multiple biological pathways and provides a different regulation mechanism from DEGs.


Introduction
When exposed to high temperatures and relative humidity, animals experience heat stress (HS) after failing to balance metabolic heat production and heat loss with a substantial increase in body temperature. Subsequently, HS reduces livestock productivity [1,2], reproduction performance [2], and immunity [3], as a consequence of using additional energy to counterbalance the excessive heat load. In general, dairy cows are more susceptible to HS due to the fact that large animals have small surface areas per unit of weight for heat dissipation [4]. Moreover, the intensive selection for high milk yield coupled with the large amount of metabolic heat generated during the process of milk production can further aggravate the state of HS [5]. As reviewed by Sammad et al. [6], HS negatively impacts the production, reproduction, health, and welfare of dairy cows around the around.
Breeding cattle with improved thermotolerance based on genetic and genomic approaches can be a feasible alternative for extensive or semi-intensive production systems [7,8], where it is more difficult to control the environmental conditions. In this regard, a better understanding of the molecular mechanisms regulating HS response is paramount when designing effective breeding programs. With the recent development and wide availability of high-throughput sequencing technologies, numerous biological pathways and candidate genes associated with HS response have been identified based on differentially expressed genes in the blood [9], mammary gland [10], and intestine [11] tissues in cattle. However, there is little known about the post-transcriptional regulation in dairy cows after exposure to HS conditions, especially studies focusing on RNA splicing.
Alternative splicing (AS) is a phenomenon involving the generation of multiple transcripts from the same gene, which enhances the translation of mRNA isoforms and proteome diversity [12,13]. This process is performed by the molecular complex known as spliceosome, which can recognize splice sites in the pre-mRNA [14]. More than one transcript can be generated by recognizing different splice sites. In humans, nearly 95% of the total multi-exon genes are involved in AS [15]. As a prevalent and tightly regulated RNA processing, AS plays a crucial role in response to environmental stresses. For instance, this mechanism contributes to the response to HS and rapidly adjusts the abundance and function of stress-response components in plants [16]. An early study related to HS-induced AS on mouse cells clarified the alternatively spliced mRNA of the HSP47 gene (heat shock protein 47) being involved in heat shock treatment [17]. Under HS conditions, AS regulates the transcriptional activity of Drosophila HSF1 (heat shock transcription factor 1) by changing the ratio of three isoforms [18]. Recently, additional studies using RNA sequencing datasets were conducted to characterize the overall changes of HS-induced AS in rice [19], fish [20], and rats [21]. To our best knowledge, genome-wide regulations of AS have not yet been studied in dairy cows under HS conditions.
In this study, we firstly compared the compressive changes in milk yield, physiological indicators, and blood biochemical parameters between dairy cows exposed to thermoneutral and heat-stress conditions to investigate the negative impact of HS on their biological processes. Subsequently, we examined the changes in AS events and their potential functions in response to HS. The integration of the results obtained with findings in differentially expressed genes in blood enabled the identification of key biological processes and candidate genes potentially involved in dairy cows' HS response. The results obtained provide a better understanding of post-transcriptional regulations in heat-stressed dairy cows and could be used as the basis for future breeding strategies.

Heat Stress Affects the Productive Performance and Physiological Indicators of Holstein Cows
The average temperature-humidity index (THI) under the thermoneutral condition (TN) was 59.23 ± 4.32 which is considered to be a comfortable state, while the average THI value during the heat-stress conditions (HS) was 81.10 ± 7.32, which exceeded the threshold of 68 [22] and was considered as moderate HS. The response characteristics of lactational performance and physiological indicators of studied subjects under HS are summarized in Table 1. Both seven-day average milk yield (7AMY, −3.65 kg/day) and milk yield on the blood sampling day (MY, −5.31 kg) were significantly decreased during the HS condition when compared to animals under TN (p < 0.05). Furthermore, the TN and HS groups had significant differences in rectal temperature (RT), respiration rate (RR), and drooling score (DS) at all time points (0700, 1400, and 2100 h) (p < 0.05), with exception of no difference in RT at the 0700 h being observed (p > 0.05). In detail, HS cows showed a statistically significantly increased RT at 1400 h (0.32 • C) and 2100 h (0.48 • C) in comparison to TN cows. For RR, HS cows had drastically higher RR of 44.48, 63.48, and 50.15 breaths per minute at the three measurement points, respectively, than that of TN cows. Similar changing patterns were investigated for DS with differences of 0.24, 0.82, and 0.42 scores between the two groups. These results indicate that the experimental cows experienced HS and responded to HS by reducing milk yield and increasing RT, RR, and DS.

Descriptive Statistics of the RNA Sequencing Data
On average, 56.43 million raw reads per sample with 97.36% of Q20 and 93.47% of Q30 were obtained. An average of 53.90 million clean reads were available for further mapping after removing poor quality reads (including very short reads, low complexity reads, and low-quality reads), and the percentages of Q20 and Q30 reached 98.08% and 94.37%, respectively. Out of these, 91.82% of high-quality reads were successfully mapped to the bovine reference genome and 88.41% of reads were identified to be uniquely mapped. All samples had unique mapping rates of over 80%. These results indicate the high quality of the RNA sequencing data obtained. A statistical summary of RNA sequencing data for each sample is shown in Table S1.

Overview of Alternative Splicing Events
Five types of AS events (Figure 2A), including skipped exon (SE), mutually exclusive exons (MXE), remained intron (RI), alternate 5 splice site (A5SS), and alternate 3 splice site (A3SS) were identified using the rMATS software. After HS, all types of AS events were increased, in which HS induced 38.9% increases in MXE and 24.4% increases in SE ( Figure 2B). In terms of the comparisons of AS events between the TN and HS groups, we found that the RI, A5SS, and A3SS events had significant overlapping (p < 0.05), while overlapping elements were not significant for MXE and SE events ( Figure 2C) (p > 0.05). Figure 2D,E show the number of SE events that occurred in each sample. The difference between the TN and HS groups was statistically significantly significant (p < 0.05). Furthermore, correlation analysis among all samples and principal component analysis (PCA) were used to investigate AS profile of the two groups based on the usage of introns (LeafCutter software), as shown in Figure 3A,B. The identified events showed a higher correlation among samples from the same group with average values of 73.50% for TN and 76.15% for HS, while an average value of 52.35% was observed among samples from different groups. Moreover, an obvious separation was observed between TN and HS in the PCA score plot of the first two principal components ( Figure 2B, PC1 = 29.74%, PC2 = 9.17%). These results emphasize the difference between the AS profiles of the two groups.

Alternative Splicing Events Differentially Expressed between the Two Groups
In total, 31,878 events corresponding to 9701 unique genes were used to perform the differential analysis. A total of 3469 differentially alternatively spliced (DS) events corresponding to 3143 unique genes (DSGs) were identified according to the criteria padjusted value < 0.05, among which 2731, 738, 114, and 20 genes annotated contained one, two, three, and four DS events, respectively ( Figure 3C, Table S2). The expression profile of the top 90 DS events is illustrated in Figure 3D, showing an expected difference between TN and HS groups. Additionally, the top 10 DS events annotated 11 genes, including TYROBP, AGTRAP, MAP3K8, ENSBTAG00000053845, BCAP31, JPT1, NT5C, CCDC107, ILK, HNRNPF, and VAMP5 (Table 2). These genes are mainly involved in the immune signaling pathways. Interestingly, all of the 11 genes had a lower percent spliced in (PSI, a score to evaluate the level of specific AS events) for the HS group.

Functional Annotation of Genes with Differentially Alternatively Spliced Events
The GO and KEGG analyses were carried out for functional annotation using 3143 DSGs. A total of 97 GO terms were significantly enriched (FDR < 0.05, Table S3), of which 58 biological processes (BP), 23 cellular components (CC), and 16 molecular functions (MF) were identified. The top 50 GO terms are shown in Figure 4A, including terms related to the immune system (including positive regulation of immune system process, immune system development, regulation of immune response, cytokine production, and cell activation), energy metabolic process (including mitochondrial envelope, mitochondrial protein complex, modification-dependent macromolecule catabolic process, mitochondrial matrix, peptide metabolic process, cellular protein catabolic process, phospholipid metabolic process, and regulation of catabolic process), gene expression regulation (including RNA splicing, mRNA metabolic process, spliceosomal complex, pre-mRNA binding, helicase activity, regulation of chromosome organization, transcription coregulator activity, catalytic activity acting on RNA, transcription factor binding, RNA localization, and DNA metabolic process), and cellular transportation (including endosomal transport and mitochondrial transport) were identified. Furthermore, 82 KEGG pathways are presented in Table S4. Figure 4B displays the top 50 pathways, including spliceosome, protein processing in the endoplasmic reticulum, neurotrophin signaling pathway, autophagy, apoptosis, and immune signaling pathway (including T cell receptor signaling pathway, B cell receptor signaling pathway, NF-kappa B signaling pathway, Th17 cell differentiation, chemokine signaling pathway, TNF signaling pathway, and C-type lectin receptor signaling pathway), and energy metabolism (including mitophagy, metabolic pathways, thermogenesis, fatty acid degradation, fatty acid metabolism, carbon metabolism, and thyroid hormone signaling pathway). Of these, 94 and 55 genes were significantly enriched in the process of RNA splicing (FDR = 1.11 × 10 −16 , Table S5) and spliceosomal complex (FDR = 1.90 × 10 −11 , Table S6), respectively, suggesting that DSGs also regulate the functions of splicing factors themselves. Figure 4C,D show that the genes involved in the splicing process are highly similar and interactive.

Differentially Alternatively Spliced Events in Differentially Expressed Genes
In total, 645 differentially expressed genes (DEGs) were identified in the comparison of TN versus HS, in which 400 genes were upregulated and 245 genes were downregulated (Table S7). Further, 87 genes were determined to be DS genes corresponding to 13.5% in DEGs while 2.77% in DSGs ( Figure 5A, Table S8). Functional annotation showed that they were most significantly enriched in the processes of gene expression regulation, such as spliceosome, RNA binding, RNA processing, RNA splicing, ribonucleoprotein complex, and RNA transport ( Figure 5B). In total, 20 corresponding genes were identified, in which 16 genes were found to be upregulated under HS and four genes were downregulated ( Figure 6A). The log-transformed relative expression fold change of four selected genes in the TN and HS groups generated from RT-qPCR were in line with the results of RNA-seq data ( Figure 6B). Further PPI network analysis demonstrated that genes involved in the first four functional enrichments were strongly related and co-expressed with each other ( Figure 6C). Among them, RBM25 and LUC7L3 can be selected as hub genes.

Gene Analysis for Differentially Alternative Spliced Events
The representative gene RBM25 (ENSBTAG00000017177) is one of the hub genes that were significantly alternatively spliced and differentially expressed under elevated temperature. Its annotated splicing events will generate two transcript isoforms in cattle ( Figure 7A), namely isoform X1 (XM_002690998.6) and isoform X2 (XM_024998128.1). In this study, as shown in Figure 7B, besides two annotated isoforms ("a" is the isoform XM_002690998.6 and "c" is the isoform XM_024998128.1), one more cryptic isoform "b" was found. At the group level, HS induced a lower proportion of the "a" isoform (70% in HS and 89% in TN) and higher proportions of the "c" isoform (13% in HS and 4.8% in TN) and "b" isoform (16% in HS and 5.9% in TN). The results of qRT-PCR also verified these detected changes ( Figure 7C). We further investigated the number of amino acids and functional domains of proteins regarding to two annotated transcripts. The residue lengths of two functional proteins (XP_002691044.2 and XP_024853896.1) are 843 and 677, respectively, and domains of RNA recognition motif (RRM), glutamate/arginine-rich sequence (ER-rich), and PWI present in XP_002691044.2 while ER-rich and PWI appear in XP_024853896.1 ( Figure 7D,F). Three-dimensional structure models also show significant differences between the two proteins.

Effects of Heat Stress on Physiological Indicators and Milk Production
HS negatively affects the health, welfare, production, and reproduction of dairy cows. When failing to lose excessive body heat, animals tend to suffer from HS [23]. Heat-stressed cows activate a variety of physiological, biochemical, endocrine, and behavioral mechanisms to counteract HS. Body temperature measurement is important in the evaluation of the stress state of dairy cows [24] and a widely accepted indicator of body temperature and thus HS [25,26]. In addition, respiration prevents hyperthermia under high ambient temperatures through the evaporation of moisture from the respiratory tract [27]. Increased respiration rate can also lead to a disturbance in body acid-base balance [6]. Thus, excessive drooling usually accompanies an increased respiration rate [28,29]. In the present study, all three indicators had statistically higher values in the HS group, suggesting the involvement of physiological thermoregulation. Milk production was significantly lower in the HS group of cows. Milk production is an energy-intensive process causing high metabolic heat production [6], which can aggravate the HS. On the other hand, the reduction in feed intake is closely related to milk production in cows subjected to chronic HS [30]. The main purpose of intake adjustment is to reduce the production of endogenous heat [31]. In light of the above discussion, it can be concluded that physiological and productive parameters indicate the presence of HS in the dairy cows included in this experiment.

Effects of Heat Stress on Blood Biochemical Parameters
The concentrations of COR and NE significantly increased while ACTH and T3 decreased in HS cows in comparison to the TN cows. In terms of ACTH and COR, they are typical stress response regulators in the hypothalamic-pituitary-adrenal (HPA) axis, COR will increase after HS exposure for a long duration [32], while decreased ACTH is related to the feedback mechanisms [33]. Moreover, the sympathetic-adrenal-medullary (SAM) axis coordinates the responses of HS by mediating the release of NE, which helps to regulate the cardiopulmonary system, such as by increasing the RR [34]. Thyroid hormones (e.g., T3) and its hypothalamic-pituitary-thyroid (HPT) axis are the components of the metabolic pathway in regulating metabolic heat production. Their lower activities cause a reduction of metabolic energy when animals are coping with high-temperature conditions [35]. Under HS conditions, other functional molecules also play an important role in response to HS. For instance, HS causes oxidative stress that stimulates certain body defense mechanisms and increases oxidative stress-related molecules such as SOD [36]. The HSP70 is activated in the presence of HS and protects cells from damage in oxidative stress [37]. Our results show that exposure to HS conditions increased both SOD and HSP70 levels in the plasma of dairy cows, which is in agreement with previous studies [36,37]. CRP as a member of the pentraxin family is known as a sensitive inflammation marker [38]. The decrease in CRP may be due to the liver dysfunction caused by HS. Considering the above, we conclude that cows suffering from HS exposure modulate the levels of hormones and functional molecules, implicating the complex regulation of HS response in dairy cows.

Effects of Heat Stress on Alternative Splicing
Heat stress response is a very complex process that involves gene regulation in multiple tissues and different molecular levels. Exposure to elevated temperature causes a sequence of changes in heat shock factors and transcriptional systems, and then alters extensive gene expression in response to HS [39]. Numerous studies have been reported on the regulatory mechanisms of HS response, particularly, focusing on the gene expression patterns and those synchronously changed networks [9,40]. Moreover, heat-inducible splicing regulation of heat-responsive genes has been also reported in different species [21,41]. In the comparison with other types of stress, the interaction between AS and HS is stronger [42]. The process for RNA splicing is regulated by cis-regulatory elements in pre-mRNA and trans-regulatory elements, mainly RNA-binding proteins (RBPs) [43]. A group of RBPs gets involved in AS regulation under HS via modulation of their own transcription and post-transcription, as well as phosphorylation status [16]. Recent reports indicate that AS is a type of positive regulatory mechanism to enable organisms to better cope with HS. For instance, grapes utilized AS to maintain the resource balance under hot conditions by adjusting their physiology and metabolism [44]. Hundreds of genes in catfish showed the involvement of RNA splicing-related genes underlying heat tolerance [41]. However, the functional roles of AS regulation in heat-stressed cows are very limited.
Accordingly, we conducted a global analysis of AS induced by HS in Holstein cows. We found that the number of AS events was higher during the HS period, which is consistent with the study in fish [41]. These stress-induced increases in AS events were also reported in rats [21] and pigs [45]. A total of 3143 unique DSGs were found between the TN and HS groups. The top genes are mainly involved in immune response and cell adaptation. These genes found in this study are important, as a recent study involving RNA sequencing of TN versus HS Holstein cows showed that immune effector response was predominant [46]. Similarly, another study investigating the effect of acute heat stress on bovine granulosa cells also found the upregulation of a high proportion of inflammation-related genes [40]. A gene involving high DS events, the ATGRAP gene, is pivotal due to its direct relationship with angiotensin II. Angiotensin II is particularly related to vasoconstriction and aldosterone biosynthesis [47,48]. Along with glucocorticoids, aldosterone initially tends to increase for homeostatic mechanisms in response to HS [49]. Furthermore, we performed the functional annotation of all DSGs. The results illustrate that these functional genes are involved in the immune system, metabolism, gene expression, and cellular transportation. This suggests that AS plays a crucial role in changing the transcriptome diversity of heat-stress-related genes in multiple biological processes. Notably, 94 and 55 genes were significantly enriched in the process of mRNA splicing and spliceosomal complex, respectively. These results are consistent with the findings in previous studies showing that splicing factors themselves also underwent regulations by AS in response to external stresses [41,50]. Therefore, DS events could regulate the functions of splicing factors themselves.
In order to better understand the regulatory mechanisms of HS, we then focused on the overlapping genes between DSGs and DEGs because experimental cows were able to cope with HS by changing both gene expression and transcript diversity. In detail, 87 DSGs (2.77% of all DSGs) related to HS were identified with differential expression at the transcriptional level and only constituted 13.5% of the total DEGs, indicating that heat-inducible AS has an independent pattern of regulation that largely differs from transcriptional regulation [21]. Therefore, there is a need for integrating the results of gene expression, gene splicing, and various regulatory mechanisms to better understand the complex transcriptional responses to HS. Surprisingly, we also found the coordinated processes in gene expression regulation (such as RNA splicing, RNA binding, and RNA transport) based on the functional analyses for those common genes, in which 20 genes were identified. Among them, RBM25, a functional protein localizes to the nuclear speckles, showed a strong interrelation and co-expression pattern with other genes. This gene is a global and crucial splicing factor due to its survival conferring role in the cells and strong conservation across eukaryotic lineages. Moreover, RBM25 interacts with components of the early spliceosome and regulators of AS [51]. In plants, RBM25 modulates response to drought stress by affecting the splicing process of the upstream gene HAB1 [52]. Human RBM25 is reported to promote apoptosis by regulating the transcripts of the gene BCL2L1 [53]. So far, the relationship between RBM25 and HS response is not well known. In the present study, the increased gene expression of RBM25 was found, indicating that it is related to molecular processes and biological functions in response to HS. Notably, the proportions of its two annotated transcripts demonstrated a significant difference between the TN and HS groups. HS induced a lower proportion of XM_002690998.6 (19%) and a higher proportion of XM_024998128.1 (8.2%). The XM_002690998.6 encodes a protein with 843 amino acid lengths containing RRM, ER-rich, and PWI domains, and XM_024998128.1 encodes 677 protein residues comprising ER-rich and PWI. All three motifs as important protein domains have the structural and functional characterization of RNA binding [53].
However, a previous study reported that RRM and PWI domains could be responsible for the interactions between RBM25 and other proteins, but they were not necessary [51]. At the same time, the ER-rich was the key domain to those protein-protein interactions [51]. Therefore, the increase of XM_024998128.1 may be the result of large demands of the RBM25 protein in the response to HS as it can produce functional protein faster. Our results reveal that this gene is a critical regulator involved in the regulation of HS response. The LUC7L3 gene, which encodes another nuclear protein with roles in pre-mRNA splicing, was also identified in this study. Its expression was found to be significantly correlated with AS events [54]. The LUC7L3 in vertebrates has two zinc finger domains, one arginine/serine-rich domain (RS), and one glutamate/arginine-rich domain (ER), in which RS is the common feature of the SR family of splicing factors [55]. LUC7L3 not only serves as a bridge between the pre-mRNA and the U1 snRNA but also interacts with RBM25 via binding the exonic element [53]. This is consistent with our results in the PPI analysis. In our case study, the increased expression pattern of LUC7L3 was investigated in HS, besides, heat-induced significant changes in its AS events were observed as well. This provides compelling insight into the important role of LUC7L3 in the presence of HS. Therefore, the RNA splicing-related genes were suggested to be regulated by the process of AS themselves [16], which may in turn facilitate the splicing of other pre-mRNAs. This study was performed on the basis of limited RNA-seq analysis and did not consider the indeed changes in translation level using the proteome platform. However, these results provide a reference for future research. Additional studies should be carried out to validate the reported differential AS events when cows are exposed to HS treatments.

Sample Collection
Animal care was undertaken in agreement with the Committee on Ethics of Animal Experimentation from the China Agricultural University (Beijing, China). A total of 36 primiparous Holstein cows at a similar lactation stage (~138 days in milk) from a commercial farm (Beijing, China) were selected as experimental animals. Experimental cows were housed in herds with an outdoor playground and fed with total mixed rations (TMR) in line with dairy feeding standard (NY/T 34-2004) and milked thrice daily (at 0700, 1400, and 2100 h). All the animals had ad libitum access to water. The temperature and humidity were recorded with an auto-recording hygrothermograph (179-THL, Apresys Inc., San Ramon, CA, USA; ± 0.3 • C and ± 3% relative humidity), and the temperature-humidity index (THI) was calculated based on the equation: THI = 0.8AT + RH × (AT-14.1) + 46.4, where AT is the ambient temperature ( • C) and RH is relative humidity [56]. The experiment was conducted during two periods based on THI, which included the thermoneutral period in April (TN, N = 19) and the heat-stressed period in July (HS, N = 17) of 2017. Approximately 10 mL of blood samples were harvested from the tail vein into anticoagulant tubes with EDTA-2K and centrifuged at 1400 × g for 15 min. Leukocytes were collected and mixed with TRIzol (No. 15596018, Thermo, Waltham, MA, USA), then stored at −80 • C until RNA isolation. Upper plasma was used to measure the biochemical parameters.

Phenotypic Measurements
Average milk yield for seven continuous days (7AMY) and daily milk yield on the blood sampling day (MY) were recorded. Rectal temperature (RT) was measured using a digital thermometer with a precision of 0.1 • C (MC-347, Omron, Tokyo, Japan) in the morning (0700-0900 h), afternoon (1400-1600 h), and evening (2100-2300 h) for three continuous days. At the same time, one trained observer recorded the respiration rate (RR) of each cow with a stopwatch according to flanking movement of the cow's body during 30 s, as well as the drooling score (DS) based on the following standard: 1, no salivation; 2, hanging or watery dripping; and, 3, drowning or inflowing [57].

Library Preparation and RNA Sequencing
The total RNA samples were isolated from leukocytes according to the manufacturer's instructions for TRIzol Reagent method [58]. RNA concentration and quality were determined using Equalbit RNA BR Assay Kit (Cat No. Q10211, Invitrogen, Carlsbad, CA, USA) and Nanodrop 2000 (Thermo, Massachusetts, USA). RNA integrity was assessed using 1% agarose gel electrophoresis, and it was used for library construction with 28S/18S >1. For the RNA-Seq library, 2 g total RNA was used for purification and fragment using NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat No. E7490S, New England Biolabs (UK) Ltd., Hitchin, Herts, UK) then followed by cDNA library with NEBNext Ultra RNA Library Prep Kit for Illumina (Cat No. E7530S, New England Biolabs Ltd., Hitchin, Herts, UK). All libraries were quantitated based on Equalbit DNA BR Assay Kit (Invitrogen, CA, USA), pooled equimolarly, and sequenced using the NovaSeq 6000 System (Illumina Inc., San Diego, CA, USA) which generates 150 bp paired-end reads.

Quality Control and Reads Alignment
The FastQC software (v0.11.9) (https://www.bioinformatics.babraham.ac.uk/projects/ fastqc/, accessed on 5 July 2021) was used to evaluate the quality of the sequencing reads, and then all reads were filtered with the adapter, less length, low complexity, and low quality, as well as global trimming using the Fastp software (v0.20.0) [59]. After that, clean reads were mapped to the bovine reference genome of version ARS-UCD1.2 using the STAR (v2.7.5c) software [60].

Differentially Expressed Gene Analyses
We investigated the counts of gene expression using the RNA-SeQC software (v2.3.6) [61]. For screening the differentially expressed genes (DEG), quantile-adjusted conditional maximum likelihood (qCML) was performed using the edgeR package in R [62] with criteria fold change (FC) ≥ 1.5 and 0.05 for the false discovery rate (FDR).

Alternative Splicing Analysis
The rMATs (v4.1.0) [63] and Leafcutter (v0.2.9) [64] software were used to identify AS events and significantly differential AS events based on the leverage information in bam files. Briefly, rMATs utilizes the proportions of exon-exon junction reads to calculate five AS events, including skipped exon (SE), mutually exclusive exons (MXE), remained intron (RI), alternate 5 splice site (A5SS), and alternate 3 splice site (A3SS). The clustering analysis of AS events in TN and HS groups was performed using the Venn2.1 package (https://bioinfogp.cnb.csic.es/tools/venny/, accessed on 31 August 2022). A hypergeometric distribution test in R software ('phyper' function) was performed to test whether the overlap was significant. Leafcutter obtains the excised introns from reads to infer exon-exon junctions. Given its high accuracy in AS quantification, Leafcutter was performed to determine the DS events between the two groups that satisfied the condition of p-adjusted < 0.05 (based on the Bonferroni adjustment, p-adjusted = p-value × the number of tests). For these novel alternative splice events, they were considered as reliable ones from which had been excised ≥ 20% of the time in ≥ 25% of the samples compared with other overlapping introns. In addition, principal component analysis (PCA), Pearson's correlation, and heatmap analysis of the two groups (TN and HS) were performed using the R software.

Functional Annotation of Differentially Alternative Splicing Genes
Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted using the online WebGestalt software (http://www.webgestalt.org/, accessed on 28 October 2021) to explore the biochemical processes and pathways that DSGs involved in. Regulators were considered to be significantly enriched based on FDR < 0.05. Protein-protein interaction (PPI) was constructed using the STRING database (https://string-db.org/, accessed on 28 October 2021).

Identification of Genes That Were Both Differentially Expressed and Differentially Alternatively Spliced
Overlapping analysis was conducted to cluster the differentially expressed genes and differentially alternatively spliced genes through Venn2.1 (https://bioinfogp.cnb.csic.es/ tools/venny/, accessed on 29 October 2021). The significant level of overlapping genes was tested based on hypergeometric analysis using the 'phyper' function in the R software. Furthermore, functional enrichment analyses and interactions of these important genes were performed using WebGestalt and STRING, respectively. The effects of AS events on their annotated transcripts and protein products can be identified based on the records in the NCBI database (https://www.ncbi.nlm.nih.gov/, accessed on 29 October 2021). These corresponding changes in functional domains and three-dimensional structure were predicted using the SMART (http://smart.embl-heidelberg.de/, accessed on 1 September 2022) and SWISS-MODEL (https://swissmodel.expasy.org/interactive, accessed on 1 September 2022) websites.

Validation of Quantitative Real-Time PCR (qRT-PCR)
Four genes were chosen for the validation of RNA-Seq results, including RBM25, PRPF40A, LARP71, and SNRNP70, and GAPDH was the housekeeping gene. The Primer-BLAST tool in NCBI (https://www.ncbi.nlm.nih.gov/tools/primer-blast/, accessed on 31 August 2022) was performed to design primers based on their mRNA sequences, and the detailed description can be found in Table S9. cDNA synthesis was achieved using a PrimeScript™RT reagent Kit with gDNA Eraser (Cat No. RR047A, Takara Biotechnology (Dalian) Co., Ltd., Dalian, Liaoning, China) conducted with 500 ng of total RNA. Validation for RNA-Seq results was conducted using quantitative real-time PCR (qRT-PCR) with SYBR Green FastFire qPCR PreMix (Cat No. FP207-02, Tiangen Biotech Co., Ltd., Beijing, China). Each reaction was performed in a total volume mixture of 20 µL consisting of 1 µL cDNA as template, 0.6 µL each of primers (10 µM), 10 µL 2 × FastFire qPCR PreMix, and 7.8 µL RNase-free ddH 2 O. The amplification protocol was as follows: 95 • C for 15 min, followed by 40 cycles of 95 • C for 10 s and 60 • C for 30 s. RNA expression levels relative to the control gene were calculated as 2 −∆∆Ct . To compare the results of the RNA-seq analysis and qRT-PCR, fold change values were log2 transformed.

Conclusions
Heat-stressed cows had a significantly higher rectal temperature, respiration rate, drooling score, and lower milk yield. In general, multiple biochemical parameters were altered during heat stress conditions in comparison to the thermoneutral period. The differentially alternatively spliced genes identified when comparing the two groups are involved in functional processes related to RNA splicing, immune system, and metabolic pathways. Moreover, low proportions of genes overlapped between differentially alternatively spliced and differentially expressed genes, highlighting their potentially independent functions in response to heat stress. Taken together, these findings indicate that alternative splicing is an important transcriptional mechanism in heat-stressed dairy cows.