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The Hidden Value of Assessing Right Ventricular Performance with Exercise in Hypertensive Patients with Left Ventricular Diastolic Dysfunction

Background: Right ventricular (RV) dysfunction could develop during exercise in patients with both hypertension and left ventricular diastolic dysfunction and may contribute to the patient symptoms. The objective is to assess RV function, both at rest and during exercise in patients with hypertension and left ventricular diastolic dysfunction. Methods: We included 30 patients with hypertension and resting LV diastolic dysfunction. The systolic function of the right ventricle was assessed by TAPSE (Tricuspid Annular Plane Systolic Excursion) and S, while E/A ratio, annular lateral E’, E/E’ and E’/A’ were used to measure diastolic function. The global function of the right ventricle was assessed by measuring the right indexed myocardial performance. The dimensions and pulmonary pressures were also measured. Results: The following parameters of RV systolic function were increased significantly with exercise: TAPSE (P = 0.0054), S’ (P = 0.0045). Moreover, the following diastolic parameters of the RV increased significantly with exercise: E/E’ (P = 0.05), A’ (P = 0.04). The global RV function showed also a significant increase (P = 0.0011). The three RV dimensions as well as the pulmonary artery pressures also increased during exercise (P = 0.000004, 0.001, and 0.00000064 respectively). In addition, the presence of resting LV grade II DD predicted significantly higher pulmonary pressures during exercise (P = 0.006). The advanced resting grade of LVDD predicted significantly the presence of advanced grade of RVDD with exercise (P = 0.037). Conclusions: Some patients who have both hypertension and LV diastolic dysfunction showed structural and functional changes of the right ventricle at rest. However, all patients had RV functional changes during exercise. How to cite this paper: Sanhoury, M., Rafla, S., El Badawy, T., & Khalil, R. M. (2021) The Hidden Value of Assessing Right Ventricular Performance with Exercise in Hypertensive Patients with Left Ventricular Diastolic Dysfunction. World Journal of Cardiovascular Diseases, 11, 261-276. https://doi.org/10.4236/wjcd.2021.116026 Received: April 23, 2021 Accepted: June 12, 2021 Published: June 15, 2021 Copyright © 2021 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/


Introduction
The impact of hypertension on the LV is very well known. However, little evidence is known about changes in the right ventricular systolic and diastolic function in patients with both hypertension and left ventricular diastolic dysfunction [1] [2] [3] [4] [5]. These changes can be attributed to: 1) Increased activity of both sympathetic and renin-angiotensin-aldosterone (RAAS) system [6] [7] [8]. 2) Hypertrophy of the septum and RV wall thickening [9] [10] [11].
Exercise is known to be a very useful technique to detect and magnify many cardiac changes.
It has been used for: • Clarification of the presence of ischemic heart disease.
• Evaluation of both systemic and pulmonary artery pressures which may be normal at rest. • Monitoring of both systolic and diastolic function during exercise.
• Evaluation of physical fitness as an initial step for medical evaluation of sports practice and prescription of cardiac rehabilitation programs [12] [13] [14] [15].
RV function increases with exercise according to Frank-starling law, due to increased preload and mild dilation of RV dimensions [16].
Aim: To assess the performance of the right ventricle, at rest and with exercise, in patients with both hypertension and left ventricular diastolic dysfunction.
Patients and Methods: Study population.
Inclusion criteria: The present study included 30 patients presented to hypertension clinic, with normal sinus rhythm, and whose transthoracic echo revealed evidence of left ventricular diastolic dysfunction, a normal systolic function of both left and right ventricles.
Exclusion criteria: We excluded patients with myocardial ischemia (based on symptoms, resting electrocardiogram (ECG) or/and normal coronary angiography if available, more than a mild disease of the heart valves, diabetes mellitus (DM), evidence of pulmonary hypertension on resting TTE, evident right ventricular pathology on resting TTE, severe tricuspid regurgitation (TR), or musculoskeletal problem precluding exercise.
Methods: All the study participants have signed informed consent. And the study was approved by the ethical committee. All the patients and control were subjected to full history taking, including age, gender, cardiovascular disease risk factors. World Journal of Cardiovascular Diseases Laboratory investigation: Lipid profile, urea, and creatinine were measured in all patients.
Resting transthoracic echocardiography: The following echocardiographic parameters were measured.
Left ventricle: Four parameters were used to diagnose diastolic dysfunction in patients with preserved ejection fraction (LVEF) [13]: Average E/E', septal E', lateral E' velocities by TDI at the mitral annulus, peak tricuspid regurgitation velocity, and left atrial volume index (LAVI). M-Mode was used to measure LVEF [17].
Devereux formula was used to measure the Left ventricular mass (LVM). LVH was graded according to 2015 recommendations on the use of echocardiography in adults with hypertension [18]. Relative wall thickness (RWT) was calculated using the following formula (2*PWT/LVEDD), PWT is the posterior wall thickness and LVEDD is the left ventricular end-diastolic dimension [18].
Right ventricle: -Systolic function was assessed by TAPSE (transannular plane systolic excursion) using M-Mode, and TDI velocities as TASV: tricuspid annular systolic velocity [19]; -Diastolic function was assessed using E/A ratio using conventional pulsed wave; Doppler, E' by TDI, E/E' ratio.
Tricuspid flow velocities are assessed by pulsed-wave Doppler in the apical four-chamber view. The following variables were determined: early diastolic peak flow velocity (E wave); late diastolic flow velocity (A wave).
TDI was used to measure myocardial velocities of the RV in the apical four-chamber view, with a sample volume placed at the tricuspid annulus lateral segment during systole (S') and early diastole (E') and tricuspid (E/E') ratio was measured [19] [20] [21].
The RV diastolic function was assessed, and the grading was done according to 2010 guidelines for the echocardiographic assessment of the right heart in adults [19].
The RV MPI (RV myocardial performance index or the Tei index) was obtained by tissue Doppler method which allows measurement of MPI as well as S', E', and A', all from a single image [20]. PAT (pulmonary acceleration time) was also recorded using pulsed-wave Doppler at the RV outlet (RVOT) in a short-axis view.
Symptom limited treadmill stress test (modified Bruce protocol): World Journal of Cardiovascular Diseases The following information was collected: resting heart rate, maximum symptom-limited heart rate, resting systolic blood pressure and diastolic blood pressure, maximum metabolic equivalent achieved at the peak of exercise. Echocardiography after treadmill stress test: An echocardiography study was performed immediately after the exercise using the same parameters previously measured.

Statistical Analysis
Data were analyzed using the Statistical Package for Social Sciences (SPSS ver20 Chicago, IL, USA). K-S test was done to check for normality of quantitative variables. Normally distributed data were expressed using mean, standard deviation (SD), minimum (min), and maximum (max  [15]. Research protocol approved from our institutional review board (IRB) and human subject informed consent.

Baseline Characteristics
The patients' mean age was 50 ± 7.1 years old (35 -63). 30% of the patients were smokers. 36.6% were receiving angiotensin-converting enzyme inhibitors (ACEI), 20% of them were receiving B-Blockers, 6% of them were receiving calcium channel blockers (CCB), 13.3% of them were receiving diuretic therapy and 24.7% of them were receiving combination therapy.
Clinical characteristics: they are shown in Table 1.
Electrocardiogram: All the patients had normal sinus rhythm on their resting ECG with no ischemic changes or arrhythmias. Laboratory Investigations: they  Table 2.
Echocardiography: Table 3 and Table 4 described the echocardiographic parameters of the patients at rest and exercise, respectively.
The following echocardiographic parameters showed significant changes during exercise (using paired t-test analysis): Left ventricle: Systolic function: -LVEF significantly increased with exercise (P value = 0.00000011), Diastolic function: the following parameters were significantly increased with exercise; -Doppler-measured trans-mitral E and A waves (P value = 0.04499 and 0.00000045, respectively). -Left atrial volume index LAVI significantly increased with exercise (P value = 0.0004).
Diastolic function: Both RV E/E' ratio and A' significantly increased with exercise (P value = 0.049 and 0.048, respectively).
Global function: RIMP was significantly increased with exercise (P value = 0.0012), while PAT was significantly decreased with exercise (P value = 0.00000067).
All three measured right ventricular dimensions were significantly increased with exercise.
The following pairs of echocardiographic parameters showed statistically significant moderate correlations: Resting E/A ratio of the LV with resting E/A ratio of the RV (r = 0.353, P value = 0.0495), exercise LV E/E' ratio to exercise RIMP (r = +0.4203, P value = 0.021) and exercise LV E/E' ratio to exercise PAT (r = −0.4011, P value = 0.028000578).
Resting-state: 1) Left ventricle: All patients had a preserved ejection fraction (EF) with a mean value of 61% ± 7%.
Regarding the diastolic function, 18 patients (60%) had left ventricular grade I  RIMP: All cases with RVDD (23 patients) showed also RIMP alteration (increased more than 0.54 by TDI at rest).

3) Correlation between RV and LV function at rest:
Systolic function: There was a weak positive correlation between the RV and LV systolic function (r = 0.096). 2) At rest, there was no statistically significant difference between the two grades of LVDD in the incidence of RVDD (P value = 0.109).
3) Furthermore, the patients who had RVDD (23 patients) had almost equal numbers of LV GI-DD and LV GII-DD cases (12 cases vs 11 cases). Therefore, among patients with RVDD (23 patients), there was no statistically significant difference between the two grades of LVDD in the incidence of a corresponding/certain grade of RVDD (P value = 0.36).
With Exercise: 1) Left ventricle: LV systolic function: In all patients, EF increased significantly during exercise (P 0.00000011). Regarding the 12 patients who had LV GII-DD at rest: 10 patients (33.33%) remained as LV GIIDD with exercise, and 2 patients (6.66%) developed G III DD.
2) Right ventricle: RV systolic function: In all patients, TAPSE and RV S' both increased significantly during exercise, (P 0.005 and 0.0045 respectively).
RV diastolic function: Exercise RVDD was altered in all patients. Ten patients (33.33%) had exercise RV GI-DD. Among them, 6 patients (20%) were in the subgroup without LVH, 2 patients (6.67%) were in the subgroup with mild LVH, and 2 patients (6.67%) were in the subgroup with moderate LVH. 18 patients (60%) had RV GII-DD. Among them, 8 of them (26.67%) had no LVH, 6 patients (20%) were in the subgroup with mild LVH, and 4 patients (13.33%) were in the subgroup with moderate LVH. 2 patients (6.67%) had RV G III-DD; both were in the subgroup without LVH.
There was no statistical significance between the presence of LVH and the incidence of RVDD at exercise (P value = 0.22). Among patients with LVH and associated exercise RVDD (14 patients), there no statistically significant association between the severity of LVH and the grade of RV diastolic dysfunction grade at exercise (P value = 0.428).

3) Correlation between RV and LV during exercise:
Systolic function: There was a positive moderate correlation between RV and LV systolic function during exercise (r = 0.33), which was not detected at rest.
Diastolic function: All 30 patients had abnormal RV diastolic function during exercise.
2) The 24 patients (80%) who had exercise LV GII-DD were distributed as follows during exercise: 4 patients developed RV GI-DD, 18 patients developed RV GII-DD and 2 patients developed RV GIII-DD.
In patients with LV GI-DD and LV GII-DD during exercise, the grade of LVDD predicted significantly the incidence of the corresponding grade of exercise RVDD (P value = 0.0046).
-In addition, the presence of resting LV GII-DD predicted significantly higher pulmonary pressures during exercise (P = 0.006). Furthermore, the advanced resting grade of LVDD predicted significantly the presence of advanced grade of RVDD with exercise (P value = 0.037).

Discussion
We studied the right ventricular performance during exertion in a group of hypertensive individuals with resting left ventricular diastolic dysfunction. We observed that among patients with LVH, the difference in the effect of LVH on the grade of the LVDD was statistically significant (P value 0.013). Furthermore, among patients with LVH, when comparing the subgroup with mild LVH and the subgroup with moderate LVH, there was a statistically significant correlation between the grade of the severity of LVH and the grade of LVDD.
Ventricular diastolic dysfunction can be present even in the absence of overt World Journal of Cardiovascular Diseases LVH in hypertensive patients. This can be caused by concentric remodeling especially in those with long = standing hypertension. This remodeling can be associated with normal or small LV cavity size decreased LV compliance and increase stiffness.
In our study, the resting systolic function of the right ventricle was preserved in all patients. This can be explained by the absence of resting overt pulmonary hypertension in these cases.
TM Gorter et al. did a meta-analysis on RV dysfunction in heart failure with preserved ejection fraction (HFpEF). They observed that RV dysfunction is mainly associated with high pulmonary artery pressures, AF, and reduced left ventricular systolic function. These criteria were excluded in our study and this may explain the preserved RV systolic function in our study [16].
According to the previously mentioned meta-analysis, RV systolic dysfunction is more prevalent in males as well as those with more severe degrees of left ventricular diastolic dysfunction, ischemic artery disease, and higher BMI [16]. In our study, the presence of LVH predicted significantly the incidence of RVDD at rest. Among patients with LVH and associated RVDD, the severity of LVH predicted significantly the grade of RVDD grade at rest.
In general, the presence of RVDD can be explained by the diastolic ventricular interdependence (shared pericardium) and the same fibrotic process involving both ventricles [16]. Furthermore, in our study, the resting E/A ratio of both ventricles had a significant moderate correlation at rest. Moreover, the LVH is normally associated with neurohormonal changes, sympathetic overflows, and activation of the RAAS system which may affect both ventricles. Cicala et al. used tissue Doppler to study right ventricular diastolic dysfunction in 30 normotensive and 30 hypertensive patients. They found that hypertension leads to RV longitudinal diastolic dysfunction (in the form of prolongation of active relaxation as a result of ventricular interaction from left ventricular pressure overload conditions) [17].
Tumuklu MM1, et al used RV standard Doppler indices, myocardial Doppler imaging, and strain/strain rate imaging indices to study the impact of both hypertension and its related LVH on the functions of the right ventricle. They found that the peak systolic strain of the RV free wall was lower than control in addition to significant alteration of the RV diastolic measurements [18].
Morris et al studied right ventricular dysfunction (both systolic and diastolic) in patients with heart failure and preserved LVEF. They found that RV systolic and diastolic dysfunction are common and possibly related to the same fibrotic processes that affect the LV and to a lesser extent with RV pressure overload [19].
In our study, all cases with resting RVDD showed also resting RIMP alteration (by TDI).

Systolic ventricular interdependence: World Journal of Cardiovascular Diseases
In the present study, a weak positive correlation between the RV and LV systolic function was observed. Around 20% -40% of RV systolic performance can be attributed to LV contraction due to sharing myocardial fibers and septum between both ventricles. This is well known as systolic ventricular interdependence.

Diastolic ventricular interdependence:
In our study, at rest, there was no statistically significant difference between the two grades of LVDD in the incidence of RVDD. Furthermore, among patients who had RVDD, no statistically significant difference between the two grades of LVDD in the incidence of a corresponding/certain grade of RVDD was observed.
Also, patients who had RVDD had an almost equal number of LV GI DD and LV GII DD (12 cases vs 11 cases). However, the incidence of RV GI-DD was higher than the incidence of RV GII-DD. This may be due to increased compliance of the RV walls compared to LV walls.
Left ventricular function with exercise: LV systolic function with exercise: In all patients, the preserved EF increased significantly during exercise. This can be explained by the increased sympathetic activation during exercise (inotropic effect, increased preload).
Furthermore, the worsening LV diastolic function during exercise generates higher Enddiastolic pressure during exercise, leading to excessive activation of the compensatory mechanisms. (Frank-starling law), and therefore increasing the stroke volume.

LV Diastolic Function with Exercise
In our study, when comparing the subgroup with mild LVH and the subgroup with moderate LVH, there was no statistical difference between the 2 groups in the incidence of a certain grade of left diastolic dysfunction during exercise.
Furthermore, when comparing the LVH group to the non-LVH group, there was no statistical difference between the 2 groups in the incidence of a certain grade of LVDD during exercise.
In addition, we found a wide spectrum of diastolic function alterations during exercise even in patients with the same grade of resting LV DD.
The study conducted by Jong-Won Ha et al. has shown the value of LV diastolic function assessment during exercise. They included 120 patients with diastolic dysfunction and borderline E/e'. All patients underwent diastolic stress echocardiography. They observed marked changes during exercise and they recommended diastolic stress echocardiography as an important tool to further assess those patients [20].
Right ventricle function during exercise: In our study, the exercise RV diastolic function was altered in all patients.
There was no statistically significant association between the presence of LVH and the incidence of RVDD with exercise. World Journal of Cardiovascular Diseases Among patients with LVH and associated exercise RVDD, there was no statistical significance between the severity of LVH and the grade of RV diastolic dysfunction grade with exercise.
The worsening of the RV diastolic function can be explained by the diastolic ventricular interdependence (shared pericardium), as the LVDD worsened during exercise too [20].

RIMP (TDI):
As all cases had exercise RVDD, they also showed a significant increase of exercise RIMP (TDI), compared with resting RIMP. Furthermore, we found a significant correlation between RIMP & LV E/E' during exercise.
Correlation between RV and LV during exercise: Systolic function: There was a positive moderate correlation between RV and LV systolic function during exercise. This correlation was found to be weak at rest.
This shows the role of exercise in unmasking the moderate correlation between the two ventricles. Therefore, this unmasked systolic interdependence can be explained by the shared septum and the shared myocardial fibers between the two ventricles.
Diastolic function: All the patients had abnormal RV diastolic function during exercise. In addition, our results showed the role of exercise in unmasking the effect of LVDD on the RV diastolic function [19] [20] [21].
However, this was not applicable for G III DD of both ventricles; this could be explained by the small number of cases who developed G III DD.
Of note, in our study, the diastolic behavioral changes of both ventricles were correlated during exercise (ventricular interaction). This was emphasized by Gorter et al. who reported that early in heart failure with preserved systolic function, RVDD could be present, at least during exercise, and in correlation with LVDD, due to possible simultaneous affection of both ventricles with the same myocardial process [21].
Effect of RV on LV: Furthermore, the relatively significant increase in RV dimensions during exercise (especially annular and mid-cavitary dimensions), as shown in Table 4, could stretch the pericardium leading to a form of pericardial constraint. The latter may in turn interfere with LV dilation & therefore can impair the LV filling and worsen the LV diastolic dysfunction with exercise (diastolic ventricular interdependence (DVI)) [21].
Study limitation: One of the limitations of our study was the small sample size. Also, we did not use other imaging modalities such as strain echocardiography, 3D imaging.

Conclusion
Patients with hypertension and left ventricular diastolic dysfunction could have right ventricular diastolic dysfunction even in absence of left ventricular hypertrophy. However, right ventricular diastolic dysfunction grade will correlate with