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Real-time functional photoacoustic remote sensing microscopy.

A fiber-tetherable non-contact photoacoustic remote sensing microscopy system capable of multiplex functional imaging is reported. By utilizing stimulated Raman scattering within an over-pumped polarization-maintaining single-mode optical fiber, rapid pulse-to-pulse switching (500 kHz) of excitation spectral content is demonstrated and utilized as a photoacoustic excitation source. These rapid acquisitions aim to reduce motion artifacts and facilitate high frame rates appropriate for real-time feedback to users. The system is characterized by estimating blood oxygen saturation in blood-flow phantoms and within a mouse ear in vivo.