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The action of nitrogen mustard in synchronized intestinal crypt cells in vivo.

The exposure to nitrogen mustard of synchronized crypt epithelial cells during various stages of the proliferative cycle induced distinctive morphological alterations in the intestinal mucosa. These structural abberrations were virtually identical regardless of the position of the crypt epithelial cells within the generation cycle at the time of drug administration and were indistinguishable from those induced in asynchronous populations. These findings indicate that nitrogen mustard can exert its cytotoxic effects not only throughout interphase but during the stage of nuclear division as well.