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Development of an Immunofluorescence Method for the Detection of Antibodies to Ebola Virus Subtype Reston by the Use of Recombinant Nucleoprotein‐Expressing HeLa Cells

An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO‐R) antibodies was developed by the use of a HeLa cell line stably expressing EBO‐R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO‐R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO‐R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO‐R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO‐R infection among monkeys.