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A stably engineered, suicidal strain of listeria monocytogenes delivers protein and/or DNA to fully differentiated intestinal epithelial monolayers.

We have generated a recombinant stable, suicidal Listeria monocytogenes strain (rsDelta2) capable of delivering antigens as protein or DNA into nondividing intestinal epithelial cells. The rsDelta2 strain was generated by inserting a cell wall hydrolysin gene, "ply118" together with its associated holin gene from a Listeria-specific phage, into the attenuated L. monocytonegenes genome of strain Delta2. The hol118/ply118 gene was placed under the control of the Listeria promoter PactA, inducing bacteria to undergo autolysis in eukaryotic cells. The rsDelta2 strain had normal growth rate in rich bacterial growth medium, but its replication in eukaryotic cells was limited, and its autolysis was used to deliver its contents to the cytoplasm of eukaryotic cells. The delivery potential of rsDelta2 was explored using engineered shuttle vectors designed to facilitate expression of a transgene, either in rsDelta2 (driven by Phly) or in the mammalian cell (driven by P(CMV)), or both (using our engineered dual Listeria and mammalian expression vector, pDuLX). The luciferase reporter was used to demonstrate that pDuLX vector allowed delivery of both protein and DNA to dividing Caco-2 human epithelial cells. As expected, nondividing fully differentiated Caco-2 monolayers were resistant to transfection with Lipofectamine, which can be explained by lack of access to the cell nucleus. We demonstrated that when Caco-2 monolayers were treated with rsDelta2, the bacteria were able to deliver a significant quantity of luciferase protein. By implication the bacteria were also able to deliver DNA, but expression driven by the eukaryotic promoter in host Caco-2 cells was not observed. When the rsDelta2 strain was taken up by Caco-2 cells, there was little or no bacterial growth, whereas the control Delta2 strain was viable and grew by approximately three log cycles within the Caco-2 cells. A small mass of protein or DNA was delivered by the Delta2 strain perhaps because some bacteria died, but despite the level of growth the mass of protein delivered to dividing Caco-2 cells by the Delta2 strain was considerably less than that delivered by the rsDelta2 strain. We concluded that the Listeria delivery system has prospects for oral vaccination using antigens synthesized by the bacterium itself.