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Detection of gamma synuclein protein in adipocytes

Gamma synuclein is a small protein that has been studied extensively in neuronal cells and in many cancers. It is highly expressed in hyperplastic tissues like breast and ovarian cancers. Its activity is thought to be in the promotion of cellular growth and proliferation. A recent report by Oort et al (2008) showed that gamma synuclein mRNA levels were detected in adipocytes during the process of maturation. We used 3T3‐L1 cells to monitor the protein levels of gamma synuclein. 3T3‐L1 preadipocytes were allowed to differentiate using a standard insulin/dexamethasone/3‐isobutyl‐1‐methylxanthine protocol. Cells were collected at various time points post‐differentiation and gamma synuclein protein was detected by western blot analysis. Appearance of peak protein levels was noted around Day 6–8 post‐differentiation and protein expression was sustained until at least Day 16. Aggregation, a characteristic shared by other members of the synuclein family, was also detected while blotting for gamma synuclein in these extracts. The sustained expression of the protein in mature adipocytes signifies a role for the protein in the differentiation process and makes the protein a possible candidate for dysregulation in obesity. This research was funded by Florida International University.