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The effects of aging on SIRT6 protein levels in various tissues of rats

The objectives of the study included examining the influence of aging on the level of SIRT6 protein in various tissues. SIRT6 is a member of the sirtuin family of proteins that function as protein lysine deacetylases and have been associated with longevity in a number of organisms. For our study, adult male Sprague‐Dawley rats, aged 6 (Adult) and 21 months (Old) were fed a standardized diet for 10–17 days. Levels of SIRT6 were measured by Western blot analysis in gastrocnemius, tibialis anterior, quadriceps, soleus, lung, heart, kidney and liver. Analyses indicated there were no significant age‐specific differences in SIRT6 protein levels in the tissues examined. However, levels of SIRT6 were significantly different between certain tissues in an age‐independent manner. SIRT6 was significantly higher in quadriceps and kidney as compared to soleus. Quadriceps, gastrocnemius, tibialis and kidney levels of SIRT6 were higher than liver. SIRT6 levels in lung and heart were lower than quadriceps. Gastrocnemius, tibialis and kidney SIRT6 levels were higher than those of lung. Heart SIRT6 levels were lower than gastrocnemius whereas tibialis and kidney levels were higher than heart SIRT6. Data from this study indicated tissue‐specific but not age‐specific differences in SIRT6 protein levels.