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[Interleukin-6 functions as an autocrine growth stimulator for a human lung adenocarcinoma cell line PAa].

OBJECTIVE
To study the effect of interleukin-6(IL-6) on the growth of a human lung adenocarcinoma cell line PAa in vitro.


METHODS
IL-6 receptor (IL-6R) mRNA level was detected by means of reverse transcription polymerase chain reaction (RT-PCR) and IL-6 mRNA was analyzed using Northern blotting hybridization. The production of IL-6 was detected by bioassay. The biological effect of IL-6 released by PAa cells was assessed with anti-IL-6 neutralizing antibodies.


RESULTS
PAa cells expressed IL-6R mRNA. Recombinant human IL-6 stimulated the proliferation of PAa cells. PAa cells also expressed IL-6 mRNA and produced bioactive IL-6. Treatment of PAa cells with anti-IL-6 antibodies resulted in decreased growth of PAa cells.


CONCLUSION
These results demonstrate that IL-6 acts as an autocrine growth stimulator for PAa cells in vitro.