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Demonstration of Viability of Perfused Human Term Placenta

The perfused, human, term placenta utilizes glucose at an average rate of 2.9 μ M/g/hr when no additional glucose is added during perfusion. The study of glucose utilization and the production of adenosinetriphosphate (ATP) by the human, term placenta perfused in vitro shows that the tissue remains metabolically active after 2½ hours of perfusion. Data for glucose utilization and ATP maintenance demonstrate active metabolism during perfusion in vitro and confirm the viability of placental tissue under these conditions.