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Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus

ABSTRACT Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination.