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AMINO ACID AND FATTY ACID COMPOSITION OF AN AQUEOUS EXTRACT OF CHANNA STRIATUS (HARUAN) THAT EXHIBITS ANTINOCICEPTIVE ACTIVITY

1 The present study was performed in order to determine the amino acid and fatty acid composition of an aqueous extract of the freshwater fish Channa striatus, obtained by soaking (1 : 2, w/v) fresh fillets overnight in a chloroform : methanol (2 : 1, v/v) solvent, to elucidate the mechanism responsible for its antinociceptive activity and to clarify the relationship between the presence of the amino and fatty acids and the expected activity. 2 The aqueous extract was found to contain all amino acids with the major amino acids glycine, alanine, lysine, aspartic acid and proline making up 35.77 ± 0.58, 10.19 ± 1.27, 9.44 ± 0.56, 8.53 ± 1.15 and 6.86 ± 0.78% of the total protein, respectively. 3 In addition, the aqueous extract was found to have a high palmitic acid (C16 : 0) content, which contributed approximately 35.93 ± 0.63% to total fatty acids. The other major fatty acids in the aqueous extract were oleic acid (C18 : 1), stearic acid (C18 : 0), linoleic acid (C18 : 2) and arachidonic acid (C20 : 4), contributing 22.96 ± 0.40, 15.31 ± 0.33, 11.45 ± 0.31 and 7.44 ± 0.83% of total fatty acids, respectively. 4 Furthermore, the aqueous extract was demonstrated to possess concentration‐dependent antinociceptive activity, as expected, when assessed using the abdominal constriction test in mice. 5 It is concluded that the aqueous extract of C. striatus contains all the important amino acids, but only some of the important fatty acids, which are suggested to play a key role in the observed antinociceptive activity of the extract, as well as in the traditionally claimed wound healing properties of the extract.