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Connective Tissue Activation. XXX: Isoelectric Point Microheterogeneity of CTAP-III, a Human Platelet-Derived Growth Factor 1

Abstract Connective tissue activating peptide (CTAP-III) is one of the growth factors found in the α granules of human platelets. This small cationic platelet protein shows several apparent isoelectric point variants after the preparative isoelectric focusing step in large scale preparations from multiple donors. Analytical isoelectricfocusing techniques under denaturing conditions, coupled with Western blotting and immunodetection, now show that CTAP-III prepared rapidly from single donors also has multiple isoelectric point variants, suggesting that this finding is more likely related to post-translational modification than preparative artifact or genetic polymorphism.