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Detection of mRNAs of TGF 1 and VEGF in Burned Corneas

METHODS. An in vitro model of angiogenesis was used to examine the roles of TNF on tube formation by human umbilical vein endothelial cells (HUVECs) cocultured with fibroblasts on induction by transforming growth factor 1 (TGF 1) and vascular endothelial growth factor (VEGF). Central cauterization was used to induce stromal neovascularization in corneas of wild-type (WT) and TNF -null (Tnf ) mice. At 7, 14, or 21 days of injury, experimental mice were killed, and the eyes were enucleated and subjected to histologic and immunohistochemical examination and real-time reverse transcription– polymerase chain reaction.