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Rapid and Frequent Somatic Embryogenesis from Single Cells of Regenerated Carrot Plantlets.

Embryogenesis was induced from regenerated carrot plantlets in a shorter time and at a higher frequency than that observed previously from hypocotyl explants of carrot seedlings (Masuda et al. J. Plant Physiol. 145, 531 (1995)). Serial observations of embryogenesis from single cells to globular embryos via cell clusters were possible in the conditioned medium. The ability to produce embryos seemed to be acquired during the transition from torpedo-shaped embryos to regenerated plantlets, when differentiation of the epidermal cells of regenerated plantlets occurred.