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Lysophosphatidic acid (LPA) Inhibits Serum Starvation Induced‐autophagy of PC‐3 Human Prostate Cancer Cell‐Line

Lysophosphatidic acid (LPA) has been shown to induce proliferation of several types of cancer cell, including human prostate cancer cell line, PC‐3. During early cancer development, cells experience serum starvation, which is a stress known to induce autophagy. Autophagy is a sub‐cellular membranes trafficking process which leads to the degradation of cellular proteins and cytoplasmic organelles. However, the relationship between LPA and autophagy is currently unclear. In this study, we demonstrated that LPA treatments inhibited autophagy of PC‐3 cells by monitoring the modification patterns of LC‐3 using Western blots. In addition, LPA decreased the LC3 labeled autophagosome in a cell line stably expressed EGFP‐LC3. Furthermore, we also showed that the inhibitory effect of LPA on autophagy was mediated through a NF‐kappa B‐dependent pathway. Our results suggested that in serum‐starved condition, LPA might facilitate early cancer development while inhibiting autophagy.