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Nucleotide sequences of the variable regions of a mouse monoclonal antibody MRK16.

MRK16 is a mouse monoclonal antibody generated against the multidrug transporter P-glycoprotein (1), which is widely used for the diagnosis and experimental therapeutics of multidrugresistant tumors. Full-length kappa and heavy chain cDNA clones were isolated from a MRK16-producing myeloma cDNA library screened respectively with the correspondent variable region genomic DNA probes (2). Nucleotide sequences of the variable region genes of the kappa (A) and heavy (B) chain of MRK16 were determined. The N-terminal twenty amino acids of the purified kappa and heavy chain proteins were sequenced and found to be identical with the predicted amino acid sequences (which are indicated by underlines).