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Pulsating blood-flow monitoring in developing fish embryos and rat mesentery by laser Doppler microscopy

Laser Doppler (LD) microscopy is a technique, providing high-resolution noninvasive measurements of microstructures dynamics. It can be used in different fields of biophysics and biomedicine. This technique yields quantitative information on diffusion coefficients, velocities, and velocity profiles of dynamic microstructures in vivo and in vitro. LD microscopy is an alternative method of velocity measurement to such methods as computer- aided microphotography and imaging, diffraction grating microscopy, FRAP, etc. In this paper we describe the results of our LDM measurements of one of the main hemodynamic parameters -- the blood-flow velocities in the microvessels of Salmo salar and Danio rerio fish embryos, as well as of the rat mesentery.