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The Transitional Endoplasmic Reticulum ATPase p97 Regulates the Alternative Nuclear Factor NF-κB Signaling via Partial Degradation of the NF-κB Subunit p100*

Background: In the alternative NF-κB signaling pathway, p100 undergoes partial degradation to generate p52. Results: Depletion of the p97-Npl4-Ufd1 complex or enzymatic inhibition of p97 significantly decreases the processing of p100 into p52. Conclusion: The p97-Npl4-Ufd1 complex positively regulates the alternative NF-κB signaling pathway. Significance: This work suggests that p97 is a potential target for diseases with dysregulation of the alternative NF-κB pathway. Partial degradation of the p100 subunit to generate p52 subunit is a hallmark of the alternative NF-κB pathway, which has been implicated in cancer. Here, we uncovered a role of the p97-Npl4-Ufd1 complex in mediating p100-to-p52 processing and therefore positively regulating the alternative NF-κB pathway. We observed an elevation of p97 mRNA levels in lymphoma patients, which positively correlates with NFKB2 expression, a downstream target gene of the alternative NF-κB pathway. Moreover, NFKB2 mRNA levels were aberrantly down-regulated in patients with inclusion body myopathy associated with Paget's disease of the bone and frontotemporal dementia (IBMPFD), a disease caused by mutation of p97. Inactivation of p97 or depletion of the p97-Npl4-Ufd1 complex inhibits the processing of p100 into p52, decreasing transcription of the downstream target genes. Further analyses reveal that the p97-Npl4-Ufd1 complex interacts with F-box and WD repeats protein SCFβTrCP complex to regulate the partial degradation of p100, a process involving K48- and K11-linked ubiquitination. In line with this, in LPS-induced lung damage mice model, generation of p52 is significantly decreased in p97-KD mice compared with mock mice. Finally, abrogation of p97 ATPase activity by its specific inhibitor DBeQ, efficiently decreased proliferation of lymphoma cells. Collectively, our study revealed a regulatory role of the p97-Npl4-Ufd1 complex in regulating p100 partial degradation, highlighting the potential of p97 as a drug target for cancers with aberrant activation of the alternative NF-κB pathway.

The diverse functions of p97 are directed by various cofactors including Npl4, Ufd1, p47, UBXD7, and FAF1 (16 -22). p97 in complex with specific cofactors is differentially involved in the degradation of multiple non-ERAD proteins. For example, the p97-Npl4-Ufd1 complex regulates the turnover of HIF1␣, Cdt1, and CD4 (18,23). Recently, p97 was also shown to regulate IB␣ degradation by promoting SCF ␤TrCP ubiquitin ligasemediated K48 polyubiquitination, leading to the activation of canonical NF-B signaling pathway (24). However, it remains unknown whether p97 is involved in the regulation of the alternative NF-B signaling pathway.
Tight control of p100 processing into p52 is important for proper function of NF-B in cell growth and survival. Dysregulation of p100-p52 processing is associated with T-cell transformation by the human T-cell leukemia virus type 1, lymphoma, and many other human cancers (33)(34)(35)(36). It is known that the SCF ␤TrCP ubiquitin ligase ubiquitinates p100 by K48-linked ubiquitination upon stimulation. However, the detailed mechanism of p100-p52 processing, especially the post-ubiquitinational events is not well defined. In this work, we found that p97 in complex with its cofactor Npl4 and Ufd1 positively regulates the alternative NF-B pathway by promoting partial degradation of p100 into p52. This process depends on the ATPase activity of p97. Mutations of p97 characterized in IBMPFD impaired such regulatory function; while enzymatic inhibition of p97 blocked the alternative NF-B signaling in cells and in mice.
Cell Culture and Transfection-HEK293T, MEF, and Raji lymphoma cells were purchased from Cell Resource Center (Institute of Life Science, Chinese Academy of Science). HEK293T and MEF cells were cultured in Dulbecco's modified Eagle's medium (DMEM), and Raji cells were maintained in RMPI 1640 medium. All culture was supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin in a 37°C humidified incubator and an atmosphere of 5% CO 2 in air. Cells were transfected with siRNA or plasmids using the liposome transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA) or Lipofectamin RNA iMax (Invitrogen) according to the manufacturer's instructions.
Immunoprecipitation and Immunoblotting-For immunoprecipitation experiments, whole cell extracts were prepared after transfection or stimulation, and incubated overnight with indicated antibodies together with protein A/G beads (Santa Cruz Biotechnology). Beads were then washed three times with lysis buffer, and immunoprecipitates were eluted with SDS loading buffer and resolved in SDS-PAGE gels. The proteins were transferred to PVDF membrane (Bio-Rad) and further incubated with the indicated antibodies.
Immunofluorescence Imaging-MEF cells were plated on coverslips in 6-well plates and transfected with the indicated plasmids. Twenty-four hours later, cells were treated with or without anti-LT␤R (100 ng/ml) for the indicated time points. Coverslips with the cells were washed once with PBS and fixed in 3.7% formaldehyde in PBS for 15 min. After permeabilization with Triton X-100 (0.25%) in PBS for 15 min, cells were blocked with PBS containing BSA (5%) for 1 h and then incubated with anti-p100/p52 antibodies for 1 h. After three separate washes, cells were incubated with secondary antibody for another hour and then stained with DAPI for 2 min. The coverslips were washed extensively and fixed on slides. Images were captured using a Leica laser scanning confocal microscope (Leica TCS SP2 AOBS).
Electrophoretic Mobility Shift Assays-The Raji cells transducing scramble or shp97 were stimulated with anti-LT␤R, and then lysed in whole cell extraction buffer containing 20 mM HEPES pH 7.9, 350 mM NaCl, 20% glycerol, 1 mM MgCl 2 , 0.5 mM EDTA, 0.5 mM EGTA, 1% Nonidet P-40, 1 mM DTT, and protease inhibitors. For antibody supershift analysis, the binding reaction was performed in the absence of the probe, the appropriate antibody was added, and the mixture was incubated for 16 h at 4°C. The probe was then added, the reaction mixture was incubated an additional 30 min at 25°C, and the complexes were resolved by gel electrophoresis.

Real-time PCR Analysis-Real-time PCR was performed on Applied Biosystems
Step Two Real-Time PCR System (Applied Biosystems) using the comparative Ct quantization method as described previously. Real-time PCR Master Mix (Toyobo, JP) was used to detect and quantify the expression level of the target gene. GAPDH was used as internal control. The primers are as the following: hNFKB2: 5Ј-ATGGAGAGTTGCTACAACCCA-3Ј (F), 5Ј-CTGTTCCACGATCACCAGGTA-3Ј (R); mNFKB2: 5Ј- Ubiquitination Assay-Cells were harvested and lysed in a buffer containing 8 M urea, 50 mM Tris, pH 7.5, 150 mM NaCl, and 1ϫ proteases inhibitor mixture. Before immunoprecipitation, the urea was diluted to a final concentration of 0.4 M with 50 mM Tris/HCl, pH 7.5. The mixture was then incubated with anti-HA-conjugated Sepharose beads overnight at 4°C. Immobilized proteins were washed five times with 1ϫ PBS buffer before being resolved by SDS-PAGE and immunoblotted with indicated antibodies. HA-K48Ub and HA-K11Ub are HA-tagged K48 only and K11 only ubiquitin mutants, respectively.
Cell Proliferation Assay-Cells were transfected with the indicated plasmids. ATP cell viability assay was used for detecting cell proliferation. ATP content was measured in accordance with the protocol of the CellTiter-Glo TM luminescent cell viability assay kit (Promega, Madison, WI). Briefly, 100 l of assay reagent was added to the wells and mixed for 2 min at room temperature. After 10 min, intracellular ATP content was measured using a luminescence multilabel counter (Envision, Perkin Elmer). Cell proliferation was calculated using the following Mice-Chow-fed, 6-week-old male C57BL/6 mice (Slac) were housed under a reverse light-dark cycle. shp97 (or control shRNA, 500 nM) in 200 l of 5% glucose, including transfection reagent jetPEI (Polyplus, France, Illkirchcedex), was intravenously injected into mice once every day for 7 days. Subsequently, animals were injected intraperitoneally with LPS (20 mg/kg) for 6 h. All procedures for animal experimentation were performed in accordance with the Institutional Animal Care and Use Committee guidelines of the Animal Core Facility of the Institutes of Biochemistry and Cell Biology (SIBCB). The approval ID for using the animals was No. 081 by the Animal Core Facility of SIBCB.
Statistics-The data are presented as means Ϯ S.D. One-way analysis of variance (ANOVA) and Student's t test were used for continuous variables. Pearson coefficient test were used for correlation analysis. p values of Ͻ0.05 were considered significant.

Results
p97 Positively Regulates p100 Processing in a Manner Dependent on Its ATPase Activity-Previously, we reported that Ter94, a Drosophila homolog of p97, regulates Hedgehog signaling pathway through partial degradation of the full-length transcriptional factor Ci155 into its active form Ci75, a process involving K11-linked ubiquitination of Ci by the Cul1-Slimbbased E3 ubiquitin ligase (37). Inspired by the similarity between Ci155-Ci75 processing and p100-p52 maturation, we hypothesized that p97 might also target p100 for partial degradation in the alternative NF-B signaling pathway. To test this possibility, we first examined the potential correlation of p97 with the alternative NF-B pathway by analyzing clinical data from the Gene Expression Omnibus (GEO) Database. We observed a significant down-regulation of NFKB2 mRNA levels in the quadriceps muscle samples of Paget disease of the bone and frontotemporal dementia (IBMPFD) patients with p97 mutation (datasets GSE30806) (p ϭ 0.0005), when compared with those of healthy people (Fig. 1A). On the other hand, no significant alteration was observed in IBMPFD samples for mRNA levels of IL6, a downstream target gene of the classical NF-B signaling (p ϭ 0.1964) (Fig. 1A). Since a previous study has implicated the alternative NF-B pathway in lymphoma (33), we also examined the potential correlation of p97 with lymphoma by analyzing clinical data from the Oncomine Database (40). An up-regulation of p97 mRNA levels was observed in the tissue samples of lymphoma patients (p Ͻ 0.05) (Fig. 1B). Moreover, p97 mRNA levels were positively correlated with those of NFKB2 in lymphoma (p Ͻ 0.01) (Fig. 1C). Together, these observations suggest that p97 is involved in the alternative NF-B signaling pathway.
To further explore the potential correlation between the IBMPFD-related mutations of p97 and the aberrant activation of the alternative NF-B pathway, we then constructed two p97 mutants commonly found in IBMPFD, namely, R155H and T262A, which have been reported to induce conformational defects of p97 in cells (41). The processing of p100 into p52 was then induced by overexpression of NF-B inducing kinase (NIK), an upstream kinase that strongly activates the alternative NF-B signaling pathway. As shown in Fig. 1, D and E, overexpression of the p97 mutant R155H or T262A dose-dependently elevated the ratio of p100/p52, and inhibited the generation of p52, suggesting a regulatory role of p97 in the alternative NF-B signaling pathway. To further validate such potential function of p97, we transfected p97 specific siRNA into HEK293T cells. Knockdown of p97 significantly elevated the ratio of p100/p52, and inhibited the generation of p52 (Fig.  1F). Meanwhile, p97 (AA), a mutant version of p97 that lost ATPase activity, also led to a marked increase in the ratio of p97 Regulates the Processing of p100 into p52 p100/p52 (Fig. 1G). Together, these data indicate that p97 indeed is involved in the processing of p100 to p52 and that such regulatory role of p97 is dependent on its ATPase activity.
Previous studies reveal that crosslinking of the lymphotoxin ␤ receptor (LT␤R) with an agonistic antibody is able to trigger p52 generation and DNA binding through inducing the processing of p100 (28). Consistently, we found that anti-LT␤R strongly induced the processing of p100 to p52 in mouse embryonic fibroblasts (MEFs) (Fig. 1H). Knockdown of p97 by its specific shRNA increased p100/p52 ratio, and resulted in a decrease in the processing of p100 after stimulation with anti-LT␤R (Fig. 1H). Moreover, ligation of CD40 can activate the alternative NF-B signaling in lymphocytes (32), we then examined the inhibitory effect of p97 on CD40L-induced FIGURE 1. p97 regulates the processing of p100 to p52 in a manner dependent on its ATPase activity. A, bar plot for the mRNA levels of NFKB2 and IL6 in the IBMPFD datasets (***, p Ͻ 0.001, n.s., no significance, same below). B, box plot for p97 mRNA levels in the lymphoma datasets from the Oncomine database (*, p Ͻ 0.05, same below). C, scatter plots between p97 and NFKB2 in the lymphoma datasets. D-E, immunoblotting for p100/p52 in cells overexpressing HA-p100 and V5-NIK with or without IBMPFD-related p97 mutants T262A (D) and R155H (E). F, immunoblotting for p100/p52 in cells overexpressing HA-p100 and V5-NIK with or without sip97 (p97 siRNA, same below). G, immunoblotting for p100/p52 in cells overexpressing HA-p100 and V5-NIK with or without p97 (AA, K251AK524A, same below) mutant. H, immunoblotting for p100/p52 in the lysates of control (scramble, same below) and p97-knockdown (shp97, p97 shRNA, same below) MEF cells after treatment with anti-LT␤R for 4 h. I, immunoblotting for p100/p52 in the lysates of control and p97-knockdown Raji cells after stimulation with CD40L for 6 h.
p97 Regulates the Processing of p100 into p52 AUGUST 7, 2015 • VOLUME 290 • NUMBER 32 p100 processing. Consistent with our observations in MEF cells, p97 depletion in Raji cells also potently blocked CD40L-induced p52 generation (Fig. 1I). Together, these data further indicate that p97 plays a positive role in the processing of p100 to p52.
p97 Facilitates the Activation of the Alternative NF-B Signaling-Activation of the alternative NF-B signaling is a multistep process that includes at least p100 processing, p52 nuclear translocation, DNA binding and transcription of downstream target genes. To further corroborate the regulatory role of p97 in the alternative NF-B signaling, we first evaluated whether IBMPFD-related mutations of p97 affect the transcription of downstream target genes of the alternative NF-B pathway. Consistent with our observations for p100-p52 processing (Fig. 1, D and E), real-time PCR analysis in anti-LT␤R-treated MEF cells showed that overexpression of p97 mutant T262A or R155H substantially reduced the mRNA levels of the alterna-tive NF-B pathway target genes NFKB2 and MCP1 (Fig. 2, A  and B). Next, we examined the effect of p97 on the nuclear translocation of p52 in MEF cells after incubation with anti-LT␤R. Immunofluorescence results demonstrated that p97 knockdown decreased the nuclear translocation of p52, when compared with scramble group (Fig. 2C). Subsequently, the effect of p97 on p52 binding to B promoter was evaluated by using a 22-bp probe containing B promoter sequence. As shown in Fig. 2D, p52 binding to the wild-type B probe (supershift) was reduced ϳ50% by silencing p97 in Raji cells. Finally, these results were further confirmed by analyzing the effect of p97 knockdown on the transcription of the alternative NF-B pathway downstream target genes. Compared with scramble groups, the mRNA levels of target genes NFKB2, MCP1, VCAM-1, and RANTES were significantly decreased by depletion of p97 in either anti-LT␤R-treated MEF or CD40L-treated Raji cells (Fig. 2, E and F). Taken together, these results clearly

p97 Regulates the Processing of p100 into p52
demonstrate that p97 facilitates the activation of the alternative NF-B signaling pathway.
p97 in Complex with Npl4-Ufd1 as a Regulator of the Alternative NF-B Pathway-The diverse functions of p97 are directed by various adaptor proteins, such as Npl4 and Ufd1. We previously showed that the Drosophila homolog of p97 acts together with Npl4 and Ufd1 to regulate the partial degradation of the transcription factor Ci in the Hedgehog signaling pathway (37). Here we also examined the functional relevance of the p97-Npl4-Ufd1 complex in the alternative NF-B signaling pathway. Similar to the effects of p97, knockdown of either Npl4 or Ufd1 significantly impaired NIK-or anti-LT␤Rinduced p100-p52 processing (Fig. 3, A and B). Consistently, depletion of Npl4/Ufd1 markedly reduced CD40L-induced p97 Regulates the Processing of p100 into p52 AUGUST 7, 2015 • VOLUME 290 • NUMBER 32 transcription of downstream target genes including NFKB2, RANTES, MCP1, and BAFF, as did p97 knockdown (Fig. 3C). Together, these observations indicate that Npl4 and Ufd1 are the cofactors required for p97 during its regulation of the alternative NF-B pathway.
To further assess the importance of the p97-Npl4-Ufd1 complex assembly on the regulation of the alternative NF-B signaling pathway, we took advantage of the available structural information and created a p97 mutant (with Phe-52, Asp-55, Tyr-110 all mutated to alanines, termed 3A) that disabled its interaction with Npl4 (42). Notably, overexpressing the mutant p97 (3A) decreased the nuclear translocation of p52 (Fig. 3D), as well as the mRNA levels of downstream target genes (Fig. 3E), suggesting a dominant negative effect of such mutation on the processing of p100 to p52. Together, these results indicate that p97 in complex with Npl4-Ufd1 is essential to regulate the alternative NF-B signaling pathway.
p97 Regulation of p100 Partial Degradation Involves SCF␤ TrCP -mediated Both K48 and K11-linked Ubiquitination-Our previous study indicates that K11-linked ubiquitination is involved in the partial degradation of the transcriptional factor Ci in the Hedgehog signaling pathway. To test whether K11-linked ubiquitination is also involved in the processing of p100 to p52 in the alternative NF-B signaling pathway, we co-transfected HEK293T cells with HA-p100 and V5-NIK. As shown in Fig. 4A, overexpression of NIK led to significant accumulation of K48-and K11-linked ubiquitination of p100; while knockdown of p97 further enhanced such effect. Consistent with this observation, transfection of the p97 (AA) mutant also enhanced NIK-induced total and K11-linked ubiquitination of p100 (Fig. 4B). Furthermore, we co-transfected Flag-p100, V5-NIK, and different shRNA with HA-K48Ub or HA-K11Ub into HEK293T cells and then evaluated p100 ubiquitination by anti-HA immunoprecipitate and subsequent anti-Flag immunoblotting. Depletion of either Npl4 or Ufd1 further increased NIK-induced accumulation of K48-and K11-linked ubiquitination of p100, as did p97 knockdown (Fig.  4C). Taken together, these results indicate that regulation of the p100 processing by the p97-Npl4-Ufd1 complex involves both K48-and K11-linked ubiquitination.
p97 binds to many ubiquitin ligases, including SCF ␤TrCP -related E3s (17). It is known that the SCF ␤TrCP ubiquitin ligase ubiquitinates p100 by K48-linked ubiquitination upon stimulation. Thus we hypothesized that p97 and the SCF ␤TrCP complex may function together to mediate the partial degradation of p100. To test this possibility, we examined interactions among p97, SCF ␤TrCP , and p100 by co-IP assay. Consistent with previous study (30), our results showed that p97 can interact with SCF components ␤TrCP and Cul1 (Fig. 4D). Moreover, both ␤TrCP and p97 interacted with p100 (Fig. 4D), suggesting that p97, together with the SCF ␤TrCP complex physically associates with p100 to regulate its partial degradation into p52. Further-more, overexpression of ␤TrCP also increased NIK-induced K48-and K11-linked ubiquitination of p100 (Fig. 4E). Taken together, these results indicate that p97 regulates p100-p52 processing by targeting p100 for partial degradation, and that this process involves SCF ␤TrCP -mediated both K48-and K11linked ubiquitination of p100.
p97 as a Potential Therapeutic Target for Cancers with Aberrant Activation of the Alternative NF-B Pathway-To further corroborate our mechanistic study of p97 regulation of the p100 processing, we developed LPS-induced lung pathology model in mock mice and p97-KD mice by intraperitoneal injection of LPS (20 mg/kg) every day (Fig. 5A). Consistent with previous reports, LPS challenge triggered the processing of p100 into p52; whereas p97 knockdown decreased the generation of p52 when compared with the mock mice (Fig. 5B). Moreover, silencing p97 impaired LPS-induced transcription of NFKB2 and MCP1 in the lung tissues of mice (Fig. 5C). These in vivo studies confirm that p97 positively regulates the processing of p100 into p52 during activation of the alternative NF-B signaling pathway.
To evaluate the potential of p97 as a therapeutic target against cancers with aberrant activation of the alternative NF-B pathway, we took advantage of p97 inhibitor compound DBeQ, which specifically turns off p97 ATPase activity. As shown in Fig. 5D, DBeQ treatment substantially blocked the processing of p100 into p52, again supporting the notion that p97 facilitates the partial degradation of p100. Moreover, treatment of the lymphoma cell line Raji with DBeQ efficiently decreased its proliferation (Fig. 5E). Consistently, such catalytic inhibition of p97 dramatically down-regulated the transcription of NFKB2 (Fig. 5E).

Discussion
Multiple functions of p97 have been studied with a common theme of "extraction and segregation of ubiquitinated substrates." Recently, our study has shown that Ter94, the Drosophila homolog of p97, regulates the Hedgehog signaling pathway by targeting the transcription factor Ci for partial degradation. In this work, we showed that p97 in complex with its cofactor Npl4 and Ufd1, regulates the alternative NF-B signaling pathway by facilitating the partial degradation of p100 into p52 in a manner dependent on ATPase activity of p97. Moreover, p97 regulation of the p100 processing involves SCF ␤TrCPmediated both K48-and K11-linked ubiquitination. These studies suggest that p97-mediated partial degradation may function as a general regulatory mechanism for the processing and maturation of certain important proteins especially transcription factors.
The NF-B subunit p100 has been long realized as a repressor of the alternative NF-B signaling pathway by inhibiting the nuclear translocation of RelB (43,44). Upon stimulation by lymphotoxin, B-cell-activating factor, or CD40 ligand, NIK and FIGURE 4. p97 regulation of the p100 processing involves both K48-and K11-linked ubiquitination. A, ubiquitination assay for p100 in p97-knockdown cells with its specific siRNA. B, ubiquitination assay for p100 in HEK293T cells after transfection with p97 (AA) mutant. C, ubiquitination assay for p100 in HEK293T cells transfected with indicated plasmids. Flag-p100, V5-NIK, and different shRNA were transfected together with HA-K48 ubiquitin (HA-K48 Ub, left) or HA-K11 ubiquitin (HA-K11 Ub, right) into HEK293T cells. Anti-HA immunoprecipitates (IP) were analyzed by immunoblotting (IB) with anti-Flag antibodies to detect ubiquitination of p100. D, co-IP assay for detection of interactions among p97, SCF ␤TrCP , and p100. E, ubiquitination assay for p100 in cells overexpressing ␤TrCP.
p97 Regulates the Processing of p100 into p52 AUGUST 7, 2015 • VOLUME 290 • NUMBER 32 p97 Regulates the Processing of p100 into p52 IKK␣ induce the phosphorylation of p100, which then interacts with the SCF ␤TrCP complex for subsequent generation of p52 (30,45,46). Our data indicate that the p97-Npl4-Ufd1 complex promotes the processing of p100 into p52 by directing p100 for SCF ␤TrCP -mediated partial degradation. In keeping with this notion, depletion of p97, Npl4, or Ufd1, disruption of the p97-Npl4 interaction, or catalytic inhibition of p97, all resulted in impaired p100-p52 processing, as well as reduced transcription of target genes. Thus an intact p97-Npl4-Ufd1 complex with ATPase activity is required for its regulatory function in the alternative NF-B signaling. Furthermore, silencing of p97, Npl4, or Ufd1, or enzymatic inactivation of p97 by abrogation of its ATP binding ability, or overexpression of ␤TrCP, all caused accumulation of K48-and K11-linked ubiquitination of p100, suggesting a functional involvement of both K48-and K11linked ubiquitination in p97-mediated partial degradation of p100. Actually, we also observed alterations of both K48-and K11-linked ubiquitination in p97-mediated processing of Ci. One possibility is that branched ubiquitin chains containing both K48-and K11-linked blocks play a role in p97-mediated protein partial degradation. In this regard, a recent paper reported that brunched ubiquitin chains containing multiple K11-linked blocks are important to provide an improved signal for proteasomal degradation (47).
Based on previous studies and our findings, we propose a mechanistic model for p97 regulation of the alternative NF-B signaling pathway (Fig. 5F). Upon activation by receptor/NIK/ IKK␣ signaling, p100 undergoes phosphorylation and recruits the SCF ␤TrCP complex and p97-Npl4-Ufd1 complex for subsequent ubiquitination and partial degradation to generate p52, which then enters the nucleus in association with RelB to activate target gene transcription. In this process, both K48-and K11-linked ubiquitin chains are added to p100 by the SCF ␤TrCP complex; while the p97-Npl4-Ufd1 complex may selectively direct p100 modified with both K48-and K11-linked ubiquitination to the proteasome for partial degradation.
Consistent with our mechanistic study of p97 in the regulation of p100-p52 processing, dysregulation of the alternative NF-B pathway was observed in IBMPFD patients with p97 mutations. We showed that IBMPFD mutation of p97 impaired its regulatory function in the alternative NF-B signaling pathway. Moreover, we found that p97 is up-regulated in lymphoma patients, and that p97 mRNA levels are positively correlated with those of NFKB2 in patients with lymphoma, further indicating the pathological relevance of p97 in the alternative NF-B signaling pathway.
It has been well established that the classical NF-B pathway has a tumor-promoting effect. Meanwhile, the alternative NF-B pathway has been implicated in the initiation of a subset of tumors including lymphoma, multiple myeloma, and melanoma (31,36,48). Regardless of their specific roles, these two pathways are usually activated concurrently in tumorigenesis. Our current work, together with previous reports now indicates that p97 can positively regulate both classical and alternative NF-B signaling. During our preparation of this manuscript, a independent research group reported the involvement of p97 in the alternative NF-B signaling pathway (49), supporting our conclusion. More importantly, we showed that the p97 specific inhibitor DBeQ can potently inhibit the viability of lymphoma and melanoma cells by down-regulating the alternative NF-B pathway, highlighting the therapeutic potential of p97 as an anticancer target, especially for patients with aberrant activation of the alternative NF-B signaling pathway.
Author Contributions-Z. Z. and Y. W. performed experiments and analyzed data. Z. S. did the structural modeling. C. L., H. Q., and W. W. contributed to data analysis. Y. Z. contributed to experimental design. Z. C. Z., Y. W,, and S. J. wrote the manuscript. Z. C. Z. and S. J. supervised the project.