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Anti-hepatocarcinoma effects of puerarin-nanoethosomes against human HepG2 cells

Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Puerarin (Pue), a major active ingredient in the traditional Chinese medicine Gegen, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Pue loaded nanoethosomes(Pue-NE) was prepared by high pressure homogenization technique. The in vitro anti-hepatocarcinoma effects of Pue-NE relative to efficacy of bulk Pue were evaluated. The particle size and zeta potential of Pue-NE were 91.8 nm and -6.1 mV, respectively. MTT assay showed that Pue-NE effectively inhibited the proliferation of HepG2 cells, and the corresponding IC50 values of Pue-NE and bulk Pue were 1.77 and 5.73 μg/ml. These results suggest that the delivery of Pue-NE is a promising approach for treating tumors.