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Sarcoplasmic Proteome Changes in Response to Aging and Diet

We seek to establish the change in the sarcoplasmic proteome the rat medial gastrocnemius (MG), as a function of age and dietary fish oil enriched diet. Physiological and behavioral measures were acquired in conjunction with quantitative two‐dimensional gel electrophoresis (2D PAGE) and immunoblotting (IB) of muscle sarcoplasm extracts. Significant proteomic changes were observed across experimental groups: young adult (8–9 months) and aged (22–23 months) S‐D rats assigned to control (Ctl) or Fish oil‐supplemented (FO) diets for 8 weeks (7 adult; 6 aged rats/group).