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Morphology of Salmonella typhi in different inactivation techniques.

Heat-phenol treatment of Salmonella typhi results in a denaturation of the cytoplasm and a smoothing of the cell wall with numerous ruptures. Following acetone inactivation the cell wall changes similarly, the cytoplasm is shrunken and its structures remain intact. Destruction is more intensive following heat-phenol treatment than after acetone precipitation. In both cases three contrasting layers of the cell wall can be seen.