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Synthesis and Characterization of Carboxymethyl Chitosan Scaffolds Grafted with Waterborne Polyurethane.

Bone tissue engineering has been rapidly developed in regenerative medicine field, which aims to induce new functional bone regeneration through the synergistic combination of biomaterials and cells. Porous biomaterials with sufficient mechanical properties and functional impregnating for bone substitutes have been imposed in the oncoming generation of bone reconstruction. In this study, we fabricated Carboxymethyl chitosan three dimensional (3D) porous scaffold modified with waterborne polyurethane (WPU) through freeze drying technique. In order to check its potential in bone tissue substitutes, osteoblast cells (hFOB 1.19) were seeded onto the fabricated scaffolds and then, SEM and proliferation assay were performed. The enhanced proliferation was contributed to 3D macroporous network structure, large surface area, and osteoconductive environments.