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Utility of sonic hedgehog and keratin 8/18 immunohistochemistry for detecting ballooned hepatocytes

Ballooned hepatocytes represent liver cell degeneration and are histological hallmarks in the diagnosis of non‐alcoholic steatohepatitis, a severe form of non‐alcoholic fatty liver disease. However, the identification of ballooned hepatocytes is often difficult, especially in the clinical setting of patients with other chronic liver diseases. In this study, we investigated the utility of immunostaining for positive sonic hedgehog (SHh) protein and negative Keratin 8/18 (K8/18) expression on ballooned hepatocytes.