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125Iodide as a surrogate tracer for epithelial chloride transport by the mouse large intestine in vitro

What is the central question of this study? The tracer 36Cl−, currently used to measure transepithelial Cl− fluxes, has become prohibitively expensive, threatening its future use. 125Iodide, previously validated alongside 36Cl− as a tracer of Cl− efflux by cells, has not been tested as a surrogate for 36Cl− across epithelia. What is the main finding and its importance? We demonstrate that 125I− can serve as an inexpensive replacement for measuring Cl− transport across mouse large intestine, tracking Cl− transport in response to cAMP stimulation (inducing Cl− secretion) in the presence and absence of the main gastrointestinal Cl−–HCO3− exchanger, DRA.