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Incorporation of fatty acids into phosphatidylcholine species by liver homogenates from chick embryos.

Labeled palmitic, stearic, oleic, linoleic and araquidonic acids were incorporated into phosphatidylcholines by incubation with liver homogenates from 13, 17 and 21 day-old chick embryos. Palmitic acid was incorporated into dienoic and tetraenoic phosphatidylcholines whereas linoleic acid was incorporated into dienoic phosphatidylcholines. Labeled palmitic and linoleic acids were specifically found into positions 1 and 2 of the phosphatidylcholine molecule, respectively. Under in vitro conditions, the incorporation of either palmitic or linoleic acids into phosphatidylcholines was 2-3 fold greater in liver homogenates from 17 day-old chick embryos than in those from 13 or 21 day-old ones.