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Sphingolipids and mitochondrial function, lessons learned from yeast

Mitochondrial dysfunction is a hallmark of several neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease, but also of cancer, diabetes and rare diseases such as Wilson’s disease (WD) and Niemann Pick type C1 (NPC). Mitochondrial dysfunction underlying human pathologies has often been associated with an aberrant cellular sphingolipid metabolism. Sphingolipids (SLs) are important membrane constituents that also act as signaling molecules. The yeast Saccharomyces cerevisiae has been pivotal in unraveling mammalian SL metabolism, mainly due to the high degree of conservation of SL metabolic pathways. In this review we will first provide a brief overview of the major differences in SL metabolism between yeast and mammalian cells and the use of SL biosynthetic inhibitors to elucidate the contribution of specific parts of the SL metabolic pathway in response to for instance stress. Next, we will discuss recent findings in yeast SL research concerning a crucial signaling role for SLs in orchestrating mitochondrial function, and translate these findings to relevant disease settings such as WD and NPC. In summary, recent research shows that S. cerevisiae is an invaluable model to investigate SLs as signaling molecules in modulating mitochondrial function, but can also be used as a tool to further enhance our current knowledge on SLs and mitochondria in mammalian cells.

The yeast S. cerevisiae (baker's or budding yeast) has been broadly exploited as a eukaryote model organism since the publication of its genome in 1996, resulting in the annotation of approximately 6000 genes located on 16 chromosomes [39]. Sequencing of the mtDNA was performed independently in 1998 [40]. In contrast, the human mtDNA sequence was already published in 1981 [41] and in 1988 the first mtDNA mutation-related human pathology was identified as Leber's hereditary optic neuropathy (LHON) [42]. LHON is characterized by optic nerve degeneration that leads to visual impairment or blindness [43]. Interestingly, approximately 31 % of the protein-coding genes in yeast have a mammalian orthologue [44] and 30 % of the genes known to be involved in human diseases may have a yeast orthologue [45,46]. Remarkably, pathways that modulate apoptosis and mitochondrial function, as well as SL metabolism, are well conserved from yeast to higher eukaryotes [47][48][49][50][51][52]. These aspects make yeast an extremely useful tool to study human diseases.
Given the numerous reports connecting SLs, mitochondrial (dys)function and human pathologies, and the position of S. cerevisiae as a model organism, we here provide an overview of literature on the interplay between SLs and mitochondrial (dys)function in the yeast S. cerevisiae and will translate these findings to relevant diseases characterized by mitochondrial dysfunction and/or aberrant SL metabolism. When we discuss yeast in this review, it typically refers to S. cerevisiae.

MITOCHONDRIA, CELLULAR POWERHOUSES
Mitochondria are double-membraned dynamic cell organelles that constantly change shape through fusion and fission [53,54] and are present in the cytoplasm of all eukaryotic cells, except mature erythrocytes [23]. The mitochondrial membranes consist of a mixture of lipids with the most abundant species phosphatidylcholine (PC), phosphatidylethanolamine (PE) and to a lesser extent cardiolipin (CL) in mammalian cells, whilst in yeast the most abundant species are PC and PE, and to a lesser extent CL and phosphatidylinositol (PI) [55]. The primordial function of mitochondria is ATP production via oxidative phosphorylation (OXPHOS). However, mitochondria also play a crucial role in the regulation of cell processes such as apoptosis and cellular ion homeostasis. For more detailed descriptions on mitochondrial function and structure, the reader is referred to [56,57].
In mammalian cells, cellular energy is mainly produced via aerobic respiration, although energy can also be generated via glycolysis in absence of oxygen, which is however far less efficient [58]. In contrast, tumor cells display high rates of glycolysis in the presence of sufficient oxygen, also known as the Warburg effect [59,60]. Interestingly, by using specific carbon sources, yeast metabolism can either be shifted towards high glycolysis, combined glycolysis and respiration, or respiration alone by forcing growth on glucose, galactose or glycerol, respectively [61,62], which is advantageous to investigate the role of respiration in a specific cellular process.
Several tools have contributed to our understanding of SL metabolism, signaling and composition in mammalian and yeast cells. For instance, mass spectrometry methods are commonly used to detect different SL species and quantify their abundance in response to various stimuli [75,76]. In addition, inhibitors of SL metabolism are routinely used to elucidate the role of SLs in various settings [77][78][79][80][81][82][83][84][85][86]. Despite the high degree of conservation of SL metabolic pathways between mammalian and yeast cells [52,87,88], there are still yeast-and mammalian-specific aspects, and particularly in biosynthetic pathways. The major yeast and mammalian SL metabolic pathways are outlined in Fig. 1.
In the following part we subsequently describe both the mammalian and yeast SL metabolism, and discuss the use of SL biosynthetic inhibitors.

Mammalian sphingolipid metabolism
In mammalian cells, the central SL Cer can be generated via either de novo biosynthesis or the salvage pathway [89] (Fig. 1 [98,99]. SM interacts with cholesterol in the plasma membrane forming SM-cholesterol-rich domains and regulates cholesterol distribution in cellular membranes and cholesterol homeostasis in cells [100]. GlycoSLs can function as receptor for carbohydrate binding proteins on the membrane to initiate transmembrane signaling events as well as cell growth, differentiation and cell-to-cell communication [101][102][103][104]. GlucosylCer and galactosylCer can either be catabolized to Cer by glucocerebrosidase [105] or galactosylceramidase [106], respectively, or serve as precursor in the formation of more complex glycoSLs. Cer also serves as precursor in the formation of low levels phytoceramide (phytoCer) by the action of dhCer desaturase [107,108]. Sphingomyelinases (SMases) break down SM to Cer [109]. Sph and dhSph are phosphorylated by Sph kinases to produce Sph-1-P and dhSph-1-phosphate (dhSph-1-P), respectively [110]. Cleavage of Sph-1-P and dhSph-1-P into phosphoethanolamine and hexadecenal, catalyzed by Sph-1-P lyase [111][112][113], represents the only exit route from the SL pathway. In turn, Sph1-P and dhSph-1-P are dephosphorylated by Sph-1-P phosphatase to yield Sph and dhSph, respectively [112,114], while Cer-1-P is dephosphorylated by Cer-1-P phosphatase generating Cer

Yeast sphingolipid metabolism
De novo SL production is conserved from yeasts to mammals up to the synthesis of dhSph ( Fig. 1) [52]. In yeast, dhSph is hydroxylated to phytoSph by sphinganine C 4hydroxylase (Sur2p) [116]. The sphingoid bases dhSph and phytosphingosine (phytoSph) are commonly referred to as long chain bases (LCBs) in yeast. Next, a fatty acid side chain is added to phytoSph or dhSph, both catalyzed by the yeast Cer synthase, i.e. Lag1p and Lac1p [117], generating phytoCer and dhCer, respectively. The former is the central SL in yeast. Sur2p-mediated hydroxylation of dhCer then generates phytoCer [116]. In addition, the yeast dihydroceramidase (Ydc1p) and yeast phytoceramidase ( [129]. For a more detailed description the reader is referred to [130].

Sphingolipid biosynthetic inhibitors
To date, the best characterized and most used inhibitors of SL biosynthesis in yeast research include Myriocin (Myr), isolated from Myriococcum albomyces and Mycelia sterilia [131]; Aureobadisin A (Aur A), isolated from Aureobasidium pullulans [132]; and Fumonisin B1 (FB1), isolated from Fusarium monoliforme [133]. Myr inhibits de novo SL biosynthesis in all eukaryotes by binding the first biosynthetic enzyme SPT [90, [134][135][136], while Aur A inhibits yeast IPC synthase [137]. FB1 inhibits Cer synthase in yeast and Inhibitors of specific SL biosynthetic enzymes are broadly exploited to synthetically affect different parts of the SL metabolic pathway in order to elucidate the origin of SLs in specific settings. A well-studied case has been the unraveling of the role of de novo SL biosynthesis during heat stress in yeast. Heat stress induces a transient cell cycle arrest in yeast [140] followed by resumption of growth at the elevated temperature [141] and several studies have implicated SLs in the heat stress response. For instance, heat stress causes a transient 2-3-fold increase of C 18 -dhSph and C 18 -phytoSph levels, an over 100-fold transient increase in C 20 -dhSph and C 20 -phytoSph, a stable 2-fold increase in C 18 -phytoSph containing Cer and a 5-fold increase in C 20 -phytoSph containing Cer [142]. Dickson and coworkers observed accumulation of the disaccharide trehalose, which is essential for protection against heat stress [143]. This effect is related to the LCB-induced expression of the trehalose biosynthetic gene TPS2 [144]. In addition, blocking synthesis of complex SLs by Aur A treatment, leading to an accumulation of LCBs and Cer, induces TPS2 expression at non-stressing temperatures. Furthermore, Aur A potentiates the effect of dhSph or heat stress on TPS2 expression [142]. Similar findings regarding heat stressinduced accumulation of LCBs and Cer were reported by Jenkins and coworkers but also that complex SLs are unaffected while Cer levels are increased, which was partially abrogated by FB1 treatment [145]. Taken together, these findings indicate a role for de novo SL synthesis during heat stress.
Several additional studies indicated that specific SL species fulfill different roles in the regulation of particular cellular responses. For instance, Jenkins and Hannun reported that LCBs are likely to be the active species to trigger cell cycle arrest during heat stress, which was confirmed as exogenous addition of either dhSph or phytoSph induces transient cell cycle arrest [146]. In addition, during heat stress de novo SL biosynthesis is responsible for LCBs and phytoCer production, while Isc1p-mediated hydrolysis of complex SLs accounts for dhCer production [147]. In addition, Δisc1 mutants display a similar cell cycle arrest as compared to the wild type strain during heat stress, indicating that Isc1p-mediated SL generation does not affect cell cycle regulation during heat stress [147]. Montefusco and coworkers addressed the specific role of distinct Cer species in SL signaling in yeast via a lipidomic and transcriptomic analysis of yeast cultures treated with different combinations of heat stress, Myr and the fatty acid myristate (C 14 ) [148]. Their results indicate that long chain dhCer species (C 14 and C 16 ) affect the expression of genes related to iron ion transportation while very long chain dhCer species (C 18 , C 18:1 , C 20 and C 20:1 ) are involved in the vacuolar protein catabolic process.
A role for stress-related SL signaling is not limited to heat stress in yeast, but has also been implicated in stress induced by toxic iron. Iron toxicity is directly related to the generation of deleterious reactive oxygen species (ROS) [149]. Lee and coworkers linked SLs to iron toxicity as high iron increases the levels of LCBs and LCB-1-Ps, and decreas-ing these levels by Myr treatment increases yeast tolerance to high iron [150]. These data point to a signaling role for LCBs and LCB-1-Ps in iron toxicity. In yeast, LCBs are known to directly phosphorylate protein kinases such as Pkh1p, which is redundant with Pkh2p and related to mammalian 3-phosphoinositide-dependent protein kinase PDK1 [151], and Ypk1p and its paralogue Ypk2p, related to serum-and glucocorticoid-inducible kinase (SGK) [151,152]. Alternatively, Ypk1/2p is phosphorylated by Pkh1p in response to LCBs. Regarding a signaling role for LCBs during iron toxicity, loss of either Pkh1p or Ypk1p indeed increases yeast tolerance to high iron [150]. Hence, LCB-based SL signaling is involved in the cellular response during iron toxicity. For additional information concerning heat and iron stress in yeast and signaling pathways mediated by LCBs the reader is referred to [150,[153][154][155][156]. Taken together, these findings suggest that SLs fulfil a crucial signaling role during various stress conditions and that specific SL species orchestrate differential responses.

CLUES FROM YEAST RESEARCH THAT LINK SLs TO MI-TOCHONDRIAL FUNCTION
The use of SL biosynthetic inhibitors in the lower eukaryotic model yeast, S. cerevisiae, has provided interesting insights into the interplay between SLs and mitochondrial function. For instance, Myr does not induce killing in yeast cells lacking mitochondrial DNA [157], i.e. ρ 0 cells, suggesting that decreased de novo SL synthesis is detrimental for cell viability and requires functional mitochondria. In addition, in yeast lifespan regulation is linked to SLs as Myr treatment extends yeast chronological lifespan (CLS), which is associated with decreased levels of LCBs, LCB-1-Ps and IPCs [78]. The yeast protein kinase Sch9p is a known regulator of longevity in yeast [158] and is activated upon phosphorylation by LCBs directly or via LCB-induced activation of Pkh1/2p [151]. In addition, Sch9p is phosphorylated by the action of the Target of Rapamycin Complex 1 (TORC1) [159], involved in nutrient signaling [159]. The reduction in SL levels upon Myr treatment during CLS was proposed to decrease activity of the Pkh1/2p-Sch9p signaling axis resulting in an increased CLS. However, a Sch9pindependent effect on CLS was also described as Myr treatment increases CLS of Δsch9 mutants [78]. Subsequently, the effect of Myr on CLS was shown to be related to its effect on the yeast transcriptome. Myr treatment during yeast ageing results in the upregulation of many genes linked to mitochondrial function and oxidative phosphorylation but also to stress responses and autophagy, and downregulation of genes related to ribosomes, cytoplasmic and mitochondrial translation, as well as to ER glycoprotein and lipid biosynthesis [160]. Hence targeting SL biosynthesis has provided insights in a link between SLs and regulating mitochondrial function.
Next to S. cerevisiae, the use of higher eukaryotic model organisms such as Caenorhabditis elegans has also significantly contributed to our current understanding of mammalian SL metabolism, and has pointed to a connection between SLs and mitochondrial function. Mitochondrial OPEN ACCESS | www.microbialcell.com defects in C. elegans are detected by a surveillance pathway, which causes the induction of mitochondrial chaperone genes such as hsp-6, but also drug-detoxification genes such as cyp-14A3 and ugt-61 [161][162][163][164]. As such, a RNA interference (RNAi) screen in C. elegans was conducted, thereby aiming at identifying genes that, upon their inactivation, renders nematodes unable to activate the mitochondrial surveillance pathway in response to mitochondrial dysfunction induced by drugs or by genetic interruption. Among their hits was sptl-1, encoding the C. elegans SPT. For instance, Sptl-1 inactivation renders nematodes unable to upregulate hsp-6 in response to inhibition of the mitochondrial electron transport by Antimycin, while no effect on hsp-6 is observed in absence of Antimycin [164]. In addition, knockout of both Cer synthase genes decreases hsp-6 induction upon mitochondrial damage while Myr prevents Antimycin-induced hsp-6p expression. Strikingly, exogenous addition of C 24 -Cer, but not dhCer or C 16 -, C 20 -or C 22 -Cer, restores the ability of sptl-1(RNAi) animals to trigger hsp-6 expression in presence of Antimycin, but not in absence of Antimycin [164]. Hence, this indicates that SLs are involved in the cellular response to mitochondrial dysfunction and that distinct SLs do serve an important signaling role in modulating mitochondrial function in higher eukaryotes in general.
In mammalian cells, the specific underlying mechanisms that connect SLs, and more specifically Cer to mitochondrial function mainly remain unclear. Nevertheless, Cer species are present in mitochondria and there are various reports that link Cer species to mitochondrial function as (i) Cer species are required for ETC complex activity, but can also inhibit ETC complexes and induce the formation of reactive oxygen species (ROS), (ii) Cer species reduces the Δψ m by mitochondrial pore formation, triggers mitochondrial outer membrane permeabilization and thus initiates apoptosis, and (iii) Cer species are determinants for the induction of mitophagy [67]. Mitophagy is a mitochondrial quality control mechanism that eliminates dysfunctional and aged mitochondria [165]. Next to these aspects (i-iii) that were recently reviewed [67], other reports that link Cer species to mitochondrial function in mammalian cells include (iv) the presence of Cer-producing enzymes in the mitochondria. El Bawab and coworkers described the identification of a human CDase that localizes to the mitochondria and is ubiquitously expressed, with the highest expression levels in the kidneys, skeletal muscles and heart [166]. Also, purified mitochondria and the mitochondriaassociated membrane from rat liver synthesize Cer in vitro via Cer synthase or reverse CDase activity [167] and there are studies describing the identification of a novel SMase that displays mitochondrial localization in zebrafish and mice as discussed below [168,169]. Lastly, in addition to the above-mentioned links between Cer and mitochondrial function (i-iv) there are (v) reports that link Cer species to mitochondrial fission events. Mitochondrial fusion is a compensatory mechanism to decrease stress by mixing the contents of partially damaged mitochondria, while mitochondrial fission is referred to as mitochondrial division in order to create new mitochondria. Both mitochondrial fusion and fission are closely involved in cell processes such as mitophagy, cell death and respiration [170]. As described by Parra and coworkers, in contrast to C 2 -dhCer, C 2 -Cer induces rapid fragmentation of the mitochondrial network in rat cardiomyocytes and increased mitochondrial content of the mitochondrial fission effectors Drp1 and Fis1 [171,172]. Additionally, inhibition of Cer synthase decreases recruitment of Drp1 and Fis1 to the mitochondria and concomitantly also reduces mitochondrial fission [173]. Moreover, Smith and coworkers showed that C 2 -Cer addition causes rapid and dramatic division of skeletal muscle mitochondria, which is characterized by increased Drp1 expression and reduced mitochondrial respiration. Interestingly, these effects are abrogated by Drp1 inhibition [174]. These reports directly link Cer species to mitochondrial fission. Taken together, there is abundant evidence that links SLs to mitochondrial function in mammalian cells.
In the following part we will first describe novel findings with regard to the SL-mitochondria connection using yeast as a model and translation of these findings to relevant higher eukaryotic settings related to mitochondrial (dys)function. We will hereby focus on Isc1p and Ncr1p, the yeast orthologue of the Niemann Pick type C1 (NPC) disease protein [175]. Also, in the context of WD, a pathological condition characterized by excess Cu and mitochondrial dysfunction [176], we will describe the potential of yeast as a model to identify novel compounds that can inhibit Cu-induced apoptosis in yeast.

Inositol phosphosphingolipid phospholipase C (Isc1p) and mitochondrial function in S. cerevisiae
In S. cerevisiae, several reports have linked SLs to mitochondrial function via the action of Isc1p [68-73]. Reportedly, Isc1p mainly resides in the ER, but localizes to the outer mitochondrial membrane during the late exponential and post-diauxic growth phase [68,71,177,178]. Isc1p is homologous to the mammalian neutral SMases (nSMase) [124]. Interestingly, a novel SMase in zebrafish cells was identified that localizes to the intermembrane space and/or the inner mitochondrial membrane [168]. Furthermore, a novel murine nSMase was reported to localize to both mitochondria and ER termed mitochondria-associated nSMase (MA-nSMase) [169]. MA-nSMase expression varies among tissues and like Isc1p in yeast [123, 179] its activity is highly influenced by phosphatidylserine and CL [169]. Though a human MA-nSMase has not yet been characterized, a putative human MA-nSMase encoding gene has been identified [180]. It is therefore conceivable that the MA-nSMase is the mammalian counterpart of yeast Isc1p, though this has yet to be elucidated as well as a putative role for human MA-nSMase in regulating mitochondrial function by modulating SL-levels.
Isc1p and its associated SL species have been extensively studied as regulators of mitochondrial function as several studies demonstrated that Δisc1 mutants display several markers of mitochondrial dysfunction such as a decreased CLS [70], the inability to grow on a non-fermentable carbon source [69,71,72,181,182], increased frequency of petite OPEN ACCESS | www.microbialcell.com formation [178], mitochondrial fragmentation [72] and abnormal mitochondrial morphology [73]. Furthermore, Δisc1 mutants display an aberrant cellular and mitochondrial SL composition as Δisc1 mutants exhibit decreased levels of all SLs with the most striking decreases in α-OH-C 24 -phytoCer and α-OH-C 26 -phytoCer species, while α-OH-C 14 -phytoCer and C 26 -phytoCer levels are increased [178]. In addition, Δisc1 mutants are characterized by decreased dhSph and α-OH-phytoCer levels and increased C 26 -dhCer and C 26 -phytoCer levels during CLS [181]. Strikingly, exogenous addition of C 12 -phytoCer allows Δisc1 mutants to grow on a non-fermentable carbon source [71]. In line with Cowart and coworkers who reported that Δisc1 mutants display aberrant gene regulation [147], Kitagaki and coworkers revealed that mitochondrial dysfunction related to loss of Isc1p is caused by a misregulation of gene expression rather than an inherent mitochondrial defect as Δisc1 mutants are unable to up-regulate genes that are involved in non-fermentable carbon source utilization, and down-regulate genes related to nutrient uptake and amino acid metabolism [182]. This points to an important signaling role for Isc1p-mediated SL generation in regulating mitochondrial function in yeast.
Currently identified downstream signaling proteins related to perturbed mitochondrial function in Δisc1 mutants include the type 2A-related serine-threonine phosphatase Sit4p [183], the mitogen-activated protein kinase Hog1p, involved in response to hyperosmotic stress [184][185][186][187], and the TORC1/Sch9p pathway [69,72,177,181]. The TORC1/Sch9p signaling pathway however is proposed as the central signaling axis to pass upstream SL signals to downstream effectors such as Hog1p and Sit4p that affect mitochondrial function [72]. It is likely that additional signaling pathways are also involved in modulating mitochondrial function in response to SLs, however, these pathways have yet to be identified. For more information concerning our current knowledge on how SLs related to the action of Isc1p are implicated in regulating mitochondrial function, and the role of the aforementioned signaling proteins the reader is referred to [74].

S. cerevisiae Δncr1 mutants, a model for Niemann Pick type C1
NPC is a fatal lipid storage disease with progressive neurodegeneration that affects 1/150.000 live births [188]. While neurodegeneration is the most prominent feature of NPC, organs such as the liver, ovaries and lungs also display aberrant lipid storage [189]. NPC is typically caused by mutations in the genes encoding NPC1 and NPC2 accounting for 95 % and 5 % of all cases, respectively [190][191][192]. NPC1 and NPC2 remove cholesterol from the late endosomes/lysosomes (LE/LY) [191,192]. Cholesterol is a sterol involved in membrane function modulation and precursor to steroid hormones, oxysterols and vitamin D [193]. NPC1deficient cells tend to accumulate lipids such as cholesterol, glycoSL and Sph in the LE/LY [79,194,195]. Despite the facts that the specific mechanisms leading to neurodegeneration in NPC are not well established, mitochondrial dysfunction and oxidative stress are found to be key charac-teristics of NPC [196][197][198][199][200]. Intriguingly, a pharmacological approach targeting glycoSL synthesis alleviates symptoms in NPC animal models [201], however, an underlying effect on mitochondrial function was not addressed. Hence, targeting SL homeostasis might be a promising approach in treatment of NPC.
Given the conservation of NPC1 and NPC2 in eukaryotes, several non-mammalian models are available to study NPC including the model yeast S. cerevisiae [202]. In yeast, Ncr1p (NPC1 related gene 1) is the orthologue of NPC1 [175] and localizes to the membrane of the vacuole [203]. The role of Ncr1p has been described as fundamentally linked to SL homeostasis with sterol movement as a consequence [175,202]. Yeast does not synthesize cholesterol, but the structural relative ergosterol [204]. Whether or not the loss of Ncr1p in yeast causes ergosterol accumulation has to be clarified yet, as Malathi and coworkers showed that Δncr1 mutants do not exhibit aberrancies in sterol metabolism [175] while more recently two independent research groups showed the contrary [205,206]. Still, intracellular sterol transport has been linked to mitochondrial function in yeast [207]. In contrast to Δncr1 mutants, mutations in the putative sterol-sensing domain of Ncr1p causes several phenotypes such as impaired growth at elevated temperatures, increased salt sensitivity and low growth on acetate and ethanol as carbon source [175]. Such phenotypes were ascribed to alterations in SL metabolism [175], as observed in NPC [79]. Although initial studies with Δncr1 mutants did not show any observable phenotype specifically related to loss of Ncr1p but rather associated with Ncr1p mutations, Berg and coworkers reported that Δncr1 mutants are resistant to the ether lipid drug edelfosine [208].
Nevertheless, Vilaça and coworkers reported very recently on phenotypes of Δncr1 mutants that at least partly resemble cellular alterations/aspects observed in NPC patients. For instance, Δncr1 mutants display increased hydrogen peroxide sensitivity and shortened CLS, with increased prevalence of oxidative stress markers [205]. Also, their results indicate that Δncr1 mutants display mitochondrial dysfunction as these mutant cells are for instance unable to grow on a non-fermentative carbon source, display decreased Δψ m and mitochondrial fragmentation [205]. In addition, Δncr1 mutants display aberrant SL homeostasis as such mutants accumulate LCBs due to increased turnover of complex SLs [205]. Taken together, in line with NPC [79, [196][197][198][199][200], Δncr1 mutants display markers of oxidative stress, mitochondrial dysfunction and accumulate SLs.
Mitochondrial function in Δncr1 mutants is suggested to be regulated by SLs. Characteristic for Δncr1 mutants is the increased Pkh1p-dependent activation of Sch9p. Concomitantly, Δncr1Δpkh1 and Δncr1Δsch9 mutants display restored mitochondrial function as these double mutants are for instance able to grow on a non-fermentable carbon source [205] essential determinants of mitochondrial dysfunction associated with NPC. Next to the above described study, yeast studies have shed light on new potential targets for treatment of NPC. Munkacsi and coworkers identified 12 pathways and 13 genes that are of importance for growth of Δncr1 mutants during anaerobis in presence of exogenous ergosterol [209]. S. cerevisiae cells become auxotrophic to sterol in absence of oxygen [210]. Based on their results, they hypothesized that histone deacetylation contributes to the pathogenesis of NPC and indeed confirmed this in NPCderived fibroblasts: genes encoding histone deacetylases (HDACs) are upregulated in NPC-derived fibroblasts. Histone deacetylase (HDAC) plays a key role in gene regulation by removing acetyl groups from specific lysine residues on histones, which increases DNA condensation and thus thereby decreases gene expression. The opposite reaction is catalyzed by histone acetyltransferases and this increases gene expression [211,212]. Intriguingly, Sph kinase 2 (SphK2), one of the two Sph kinase isoforms which mainly localizes to the nucleus [213], has been shown to associate with HDAC1 and HDAC2, two class I HDACs [214], in repressor complexes as well as histone H3 and thereby increasing H3 acetylation and transcription. This increase in H3 acetylation and transcription is attributed to the SphK2dependent Sph-1-P production in the nucleus which directly binds to the active site of HDAC1 and HDAC2, and thereby inhibits their activity and linking SLs to gene expression [215]. In addition, Munkacsi and coworkers could reverse aberrant HDAC function and show concomitant improved NPC characteristics such as decreased accumulation of cholesterol and SLs [209]. In line, HDAC inhibitors were recently suggested as a promising therapeutic in treatment of NPC [216]. Hence, the study by Munckacsi and coworkers indicates that S. cerevisiae is a powerful tool to identify novel pathways involved in the pathogenesis of NPC and for selecting novel therapeutic targets and therapies.

S. cerevisiae as a model to study Cu toxicity in context of Wilson disease
WD is a relevant human pathology (incidence 1/30.000) caused by mutations in the gene encoding the Cutransporting ATPase ATP7B resulting in the accumulation of excess Cu in the liver and increased intracellular Cu levels [176,[217][218][219]. This results in acute liver failure or cirrhosis but also neurodegeneration [217,218,220]. Interestingly, the yeast CCC2 gene, encoding a P-type Cutransporting ATPase, is homologous to ATP7B [221]. Cu uptake in yeast is mediated by the high-affinity Cu transporter Ctr1p [222] and Cu is subsequently delivered to Ccc2p by the action of the Cu metallochaperone Atx1p [223]. Ccc2p transports Cu to the Golgi lumen for Cu incorporation into Fet3p, which is required for iron uptake [224]. Loss of Ccc2p results in respiration defects and defective iron uptake [224,225]. Also, Δccc2 mutants exhibit defective growth on low iron-containing growth media which can be rescued by overexpression of wild type ATP7B or WD-related ATP7B mutants [226,227]. However, ATP7B mutants do not restore Δccc2 mutant growth on low iron-containing growth medium to the same extent as wild type ATPB [226,227]. Mechanistic events that are characteristic for Cu-induced toxicity in liver cells is Cu-induced mitochondrial dysfunction [6] and Cu-induced increased acid SMase (aSMase) activity [30]. The latter study showed that Cu increases aSMase acitivity resulting in increased levels of pro-apoptotic Cer [30,228]. In addition, their results show that aSMase inhibition, either by pharmacological intervention or genetic disruption prevents Cu-induced apoptosis [30]. Interestingly, there is an increased constitutive activation of aSMase in plasma of WD patients. Thus, Cu-induced toxicity is fundamentally linked to mitochondrial dysfunction and aberrant SL metabolism.
We recently showed that the A. thaliana-derived decapeptide OSIP108 [229] prevents Cu-induced apoptosis and oxidative stress in yeast and human cells [230], but also prevents Cu-induced hepatotoxicity in a zebrafish larvae model (unpublished data). Based on the observation that OSIP108 pretreatment of HepG2 cells was necessary in order to observe anti-apoptotic effects, we investigated the effect of OSIP108 on SL homeostasis in HepG2 cells and found that OSIP108-treated HepG2 cells displayed decreased levels of sphingoid bases (Sph, Sph-1-P and dhSph-1-P), dhCer species (C 12 and C 14 ), Cer species (C 18:1 and C 26 ) and SM species (C 14 , C 18 , C 20:1 and C 24 ). Of note is that dhSph levels in OSIP108-treated HepG2 cells were also decreased but not to a significant level. These observations led to the hypothesis that OSIP108 might act as a 3ketodihydrosphingosine reductase inhibitor. Hence, we subsequently validated these observations in S. cerevisiae and found that exogenous dhSph addition abolished the protective effect of OSIP108 on Cu-induced toxicity in yeast cells [230]. As exogenous dhSph abolished this protective effect, this suggests that SLs are directly involved in Cuinduced toxicity in yeast and mammalian cells, and that compounds that can rescue Cu-induced toxicity in yeast seem to specifically target SL homeostasis. There is however not yet conclusive evidence to support this hypothesis.
In addition, our ongoing research is aimed at identifying novel compounds that increase yeast tolerance to suggested inducers of mitochondrial dysfunction, including Cu. As such, by screening the Pharmakon 1600 repositioning library, we identified at least 1 class of off-patent drugs that prevent Cu-induced toxicity in yeast (unpublished data). Thus far, this drug class has not been linked to Cu toxicity, nor does their mammalian target have a yeast counterpart. We are currently translating these data to a higher eukaryotic setting. Hence, this indicates that our Cutoxicity yeast screen can result in the identification of new novel therapeutic options and unknown targets in treatment of, for instance, WD.

CONCLUSION
In conclusion, several studies in S. cerevisiae indicate an important signaling role for SLs in maintaining correct mitochondrial function. These data were confirmed in relevant mammalian models for pathologies characterized by mitochondrial dysfunction. More specifically, knowledge OPEN ACCESS | www.microbialcell.com on the link between SLs and mitochondrial function generated in the model yeast S. cerevisiae advanced research in particular diseases such as WD and NPC. In addition, using yeast as screening model for these diseases, development of novel therapies seems feasible and promising.
Noteworthy is, however, that different SL species clearly have different roles as exemplified by the differential effect of Cer species with different chain length on the induction of the mitochondrial surveillance pathway in C. elegans [164]. Moreover, the differential role of Cer species with different chain length in human diseases was discussed recently [231]. As for yeast research, the study by Montefusco and coworkers showed that specific groups of Cer species that vary in side chain and hydroxylation coordinate different sets of functionally related genes [148]. Thus, besides the fact that different Cer species are subjected to regulation by specific biochemical pathways in specific subcellular compartments, they also serve distinct roles, which was discussed previously by Hannun and Obeid [89]. In the latter review article, the interconnectivity of the SL metabolism was also highlighted, given the fact that manipulating one enzyme involved in SL metabolism not only leads to the perturbation of its derived SL metabolite, but also to downstream derived SL species, denoted as the 'metabolic ripple effect'. Hence, despite our extensive knowledge on SL metabolism and functioning, the concept of many ceramides and the interconnectivity of SL metabo-lism introduces additional complexity in tackling the roles for specific SL species in SL signaling.
In conclusion, basic yeast research has provided important clues for SL signaling events that impact on mitochondrial function, in higher eukaryotic and mammalian cells, as well as for novel therapeutic options for diseases in which mitochondrial dysfunction is critical.   OPEN ACCESS | www.microbialcell.com