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Computational analysis using CisFind of the 3'UTR of cytosolic Glutamine synthetase genes involved in posttranscriptional regulation

Glutamine synthetase (GS) is a key enzyme in nitrogen metabolism in plants. One of the GS isoforms in plants, GS1, is transcriptionally regulated. Our recent studies show that a soybean GS1, Gmglnßl, gene is posttranscriptionally regulated by its 3'UTR. Using a transgenic approach, we have shown that the 3'UTR of one of the two alfalfa GS1 genes, MsGS100, plays a similar role as the 3'UTR of Gmglnßl gene in posttranscriptional regulation of a reporter gene while the 3'UTR of the other alfalfa GS1 gene, MsGS13, does not. The transgenic data was verified using CisFind, and the results showed that the MsGSl00 and Gmglnßl with similar posttranscriptional regulation pattern shared common putative cis-elements in their 3'UTRs whereas these elements were absent in MsGS13 3'UTR that did not exhibit similar posttranscriptional regulation pattern. CisFind also detected putative cis-elements that are overrepresented in the 3'UTR of nodule-enhanced GS1 genes.