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Rapid Analysis of Xanthophylls in Ethanol Extracts of Corn by HPLC

Abstract A rapid HPLC method for the identification of xanthophylls in ethanol extracts of corn was developed. This method requires only the extraction of xanthophylls using aqueous ethanol followed by injection of the microfiltered extract onto a C30 column. The solvent system consists of pure methanol and MTBE. Initial mobile phase concentration, gradient slope, and flow rate were optimized to reduce analysis times by 60% compared to the existing method while maintaining the minimum desired resolution.