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A fluidic system for photometric dissolved oxygen measurements with enhanced sensitivity

A new method of color pixel intensity analysis to obtain an oxygen concentration is presented in this research. Until recently, color charge coupled devices (CCDs) have rarely been used for oxygen imaging in spite of its usefulness for analyzing the spectral content of images. The proposed new method involves extracting the red color element to enhance oxygen-related information and eliminate distorted green color information from the color images of the sensors. A commercial RedEyeTM oxygen sensor patch was used to verify this method. The linearity and sensitivity of oxygen detection based on the red intensity analysis was improved to those of spectrometric measurement and total color intensity analysis. This method also has potential applications in other luminescence sensors and simultaneous structural and functional imaging of biological systems.