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Nitrite Reductase Activity of Deoxy Carcinus maenas Hemocyanin: Formation of the Half-Met Derivative.

The kinetics of the reaction between nitrite and excess deoxy hemocyanin (Hc) is presented. The rate of disappearance of both reactants is equal to the rate of formation of the products, half-met hemocyanin and NO. Quantitative analyses accounted for all species in the following reaction, in which oxidation of one Cu(I) per binuclear site occurs upon single electron oxidation by nitrite.