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Strategy for Preparative Separation of Quaternary Alkaloids from Chelidonium majus L. by Thin-Layer Chromatography

A Chelidonium majus L. quaternary alkaloid fraction has been used as a model mixture to investigate the effect of the procedure used on the preparative separation of its components. The steps optimized were: the mode of application of the starting band to the layer; the effect on the separation of the bands of the number of developments in unidimensional multiple development (UMD); the effect of concentration overloading on the separation of the bands; and the effect of the volume of solution introduced to the adsorbent layer on the resolution of neighboring bands.