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Bilingual Processing Mechanisms of Scientific Metaphors and Conventional Metaphors: Evidence via a Contrastive Event-Related Potentials Study

To study the different mechanisms of understanding figurative language in a speaker’s native language (L1) and their second language (L2), this study investigated how scientific metaphors in Chinese (L1) and English (L2) are electrophysiologically processed via event-related potential experimentation. Compared with the metaphors from daily life or in literary works, scientific metaphors tend to involve both a more complicated context structure and a distinct knowledge-inferencing process. During the N400 time window (300–500 ms), English scientific metaphors elicited more negative N400s than Chinese ones at the parietal region. In the late positive component (LPC) time window (550–800 ms), English scientific metaphors elicited less positive LPCs than Chinese ones at the parietal region, and larger late negativities encompassing smaller areas of the brain. The findings might indicate that for late unbalanced bilingual speakers, L2 scientific metaphor comprehension requires more effort in information retrieval or access to the non-literal route. Altogether, the possible findings are that non-native and non-dominant language processing involves decreased automaticity of cognitive mechanisms, and decreased sensitivity to the levels of conventionality of metaphoric meanings.


INTRODUCTION
The semantic integration between the daily source domain and the scientific target domain involved in scientific metaphor processing provides a perspective for exploring the similarities and differences between processing mechanisms of native language and non-native language (Jankowiak et al., 2017). However, previous studies focused on conventional metaphors and devoted little attention to scientific metaphors. Such metaphors are frequently deployed by scientists who use concrete and intuitive objects and knowledge to describe abstract concepts or objects that are impossible to observe directly. The mechanism of scientific metaphors reflects the core idea of conceptual metaphor theory, the metaphorical understanding of abstract concepts through more specific knowledge structures (Lakoff and Johnson, 1980). The continuous innovation of natural science requires the reorganization and construction of thinking and expression methods in systems related to new concept expression (Tang et al., 2017a,b). Compared with conventional metaphors, the processing of scientific metaphors involves a unique mechanism involving a complicated contextual structure and knowledge inference, which might provide insight in observing the between-language differences of the brain correlates modulated by nativeness, thus deepening the comparative study of bilingual processing mechanisms. Moreover, the comparative study of two different types of metaphors (scientific metaphors and conventional metaphors) might shed new light on the discussion of the processing mechanism of figurative language.
The processing of scientific metaphors differs from that of conventional metaphors mainly in two aspects. First, the contextual structure of scientific metaphors is more complex than that of conventional metaphors, which has been established in previous N400 experiments (Tang et al., 2017a). For example, the source domain (code) of the scientific metaphor (A mitochondrion is a code) is more frequently used in everyday contexts, while the target domain (mitochondria) is more frequently used in scientific contexts. Second, the late processing period of scientific metaphors probably involves a reasoning process from the daily concrete concept to the scientific abstract concept, so as to better comprehend the embedded scientific knowledge. The late components of ERP [the late positive component (LPC) and the late negativity] are very sensitive to this process.
Event-related potentials (ERPs) with extremely high time resolution and rich component dimensions are increasingly used in the study of metaphorical cognitive neural mechanisms. The N400 is considered to be very sensitive to semantic violations (Kutas and Hillyard, 1980), and is an important index to measure the difficulty in retrieving information stored in the mind. Several monolingual studies on figurative meaning processing reported a modulation of N400 amplitudes by the degree of metaphor conventionality (Pynte et al., 1996;Tartter et al., 2002;Coulson and Van Petten, 2007;Lai et al., 2009). Such results seem to be in line with the Career of Metaphor Model (Bowdle and Gentner, 2005), which postulates that novel metaphors are understood through comparison, while conventional metaphors are preferentially analyzed by categorization. Namely, the processing of novel metaphors involves structure alignment between a metaphoric target and a literal base. In contrast, when processing conventional metaphors, the metaphoric target concept is understood as a member of a superordinate category specified by the literal base term (Jankowiak et al., 2017). Thus, understanding novel metaphors requires engagement in sense creation, while understanding conventional metaphors involves easier sense retrieval, as revealed by enhanced N400 amplitudes for novel metaphors (Arzouan et al., 2007a;Lai et al., 2009). In addition to novelty, N400 is also sensitive to concreteness. Forgács et al. (2014Forgács et al. ( , 2015 proposed that most metaphors describe abstract concepts, which, in their study, elicited larger N400s compared with abstract literal expressions when stimulus novelty was controlled across types. In this study, the target words of scientific metaphors were scientific terms and were relatively more abstract than the daily targets of conventional metaphors, which might contribute to the enhanced N400 amplitudes. The late positive component (LPC) is considered to reflect integration or reprocessing at the sentence level (Kaan et al., 2000). Some monolingual studies found that novel metaphors elicited greater LPCs than conventional metaphors (De Grauwe et al., 2010;Weiland et al., 2014), which indicates that the semantic processes of metaphorical sentences require additional retrieval of information from semantic memory. Other monolingual studies found that novel metaphors elicited smaller LPCs than conventional metaphors (Arzouan et al., 2007a,b;Zhao et al., 2011;Ma et al., 2016), which might be caused by a late negativity overlapping in the LPC time window, reflecting the secondary processing of further semantic integration. Such late negativity is also considered as a continuation of the N400, indicating a sustained difficulty in fusing two concepts from the source domain and the target domain (Goldstein et al., 2012;Rutter et al., 2012).
So far, few studies focused on the processing mechanism of scientific metaphors for bilingual speakers. Thus, expanding a monolingual electrophysiological study on scientific metaphor processing to include a bilingual component might be insightful to enunciate conceptual integration mechanisms when processing semantically complex meanings. Some bilingual ERP studies have reported a lower N400 amplitude elicited by L2 relative to L1 stimuli (Proverbio et al., 2002;Moreno et al., 2008;Midgley et al., 2009;Newman et al., 2012;Heidlmayr et al., 2015), probably indicating weaker interconnectivity for L2 words within the semantic network compared with L1 words (Midgley et al., 2009). Consequently, when processing non-native language, the activity in long-term memory decreases due to weaker interconnectivity. The functional role of the N400 effect is linked to memory operations involved in information retrieval (Kutas and Federmeier, 2000;Kotz et al., 2012). The current study seeks to build on existing research as to semantic processing in bilinguals by using materials with different semantic complexity ranging from highly complex (scientific metaphoric) and relatively complex (conventional metaphoric) to relatively simple (literal) utterances. To minimize the potential influence of any between-group individual differences, a withinsubject design was adopted. Accordingly, contrastive analyses of cognitive mechanisms were carried out to investigate native and non-native figurative language comprehension, which can further reveal how semantic complexity and language dominance interact with each other when processing bilingual languages.
The main objective of this study was to observe brain responses to scientific metaphors, conventional metaphors, and literal expressions in Chinese (L1) and English (L2). First, we aimed to observe whether scientific metaphors would evoke higher N400 amplitudes compared with conventional metaphors in both L1 and L2. That is to say, our first focus was the modulation of conventionality for the N400 effect in two languages. A linear N400 effect has been reported by two previous monolingual studies (Tang et al., 2017a,b), with more pronounced N400 amplitudes for scientific metaphors relative to conventional metaphors. This increased processing difficulty was evident due to more demanding mappings required for scientific metaphor comprehension when complicated context and scientific reasoning are engaged. Second, we aimed to observe whether English scientific metaphors would elicit attenuated N400 effects in comparison to Chinese ones, as has been found in previous bilingual research on semantic processing (Weber-Fox and Neville, 1996;Proverbio et al., 2002;Phillips et al., 2004;Moreno and Kutas, 2005;Midgley et al., 2009;Braunstein et al., 2012;Newman et al., 2012;Heidlmayr et al., 2015). Lower N400s elicited by English materials compared with Chinese ones would further imply weaker semantic interconnectivity for L2 when compared with L1 stimuli (Midgley et al., 2009). Third, we aimed to examine whether language nativeness would modulate cognitive mechanisms involved in semantic reintegration at the later stage of processing, as indexed by the LPC response. Several monolingual studies have reported higher LPC amplitudes as the index of secondary semantic integration (van Herten et al., 2005;De Grauwe et al., 2010;Brouwer et al., 2012;Rataj, 2014). It was expected that English scientific metaphors would elicit decreased LPC amplitudes in comparison to Chinese ones. Lower LPCs elicited by English materials relative to Chinese ones would point to weaker semantic processing for L2 compared with L1 sentences (Newman et al., 2012). Furthermore, if the LPC effect is modulated by the conventionality of metaphors (Arzouan et al., 2007a), scientific metaphors should evoke more pronounced LPC amplitudes than conventional metaphors, indicating the continuation of information retrieval or access to the non-literal route. Finally, we expected that main effects between LPCs across items could be observed in both L1 and L2, indicating a similar sensitivity to different levels of conventionality of metaphors.

Participants
In total, 20 participants (right-handed, healthy, undergraduate students, L1-Chinese) took part in the ERP experiment. They had either normal or corrected vision and no history of mental illness, neurological disorders, or severe brain damage. All subjects started to learn English from elementary school and have passed the CET-6. All subjects signed a consent and confidentiality agreement before the experiment and received remuneration after the experiment. Finally, the trial data of three subjects were eliminated due to failure to meet the 80% completion-rate threshold. Therefore, the final number of subjects included in our statistical analysis was 17 (7 men, 10 women, average age 21.3 ± 5.32 years).

Stimuli
The stimulus pool consisted of 120 sentences, which fell into three categories, namely, scientific metaphors, conventional metaphors, and literal sentences, with 40 sentences in each sentence category. This pool matched the one used in our previous study (Tang et al., 2017a,b) (refer to Table 1 for details).

Procedure
The experiment took place in a sound-attenuated, electrically shielded room. Participants were seated 80 cm away from the display screen. The sentences were presented in white color on a black background, word by word in a quasi-random order. As illustrated in Figure 1, stimuli in each trial were presented in the following time sequence: fixation cross (800 ms), blank (200-500 ms), subject (1,000 ms), verb (600 ms), blank (200-500 ms), object (1,000 ms), and question mark (3,000 ms). At the sight of the question mark, participants were asked to do a semantic judgment by pressing a corresponding key with right and left index fingers. The whole experiment consisted of four blocks interspersed with three rest intervals. To familiarize the subjects with trial procedure and operation, a practice session was done before the formal experiment. To mitigate any difficulty in understanding the scientific terms involved in the stimuli, participants were first asked to read a list covering all the scientific terms used.

EEG Recording and Data Analysis
Scalp voltages were collected using the CURRY 7 system (Compumedics Neuroscan, Texas, United States) with 64 Ag/AgCl electrodes, monitored using the CURRY recording software and connected to a SynAmp amplifier (Compumedics Neuroscan, Texas, United States). Amplified analog voltages were digitized at 1,000 Hz. Impedances of individual sensors were kept below 5 k . Eye movements were monitored through bipolar electrodes, which were placed above and below the right eye, as well as at the left and right canthi. Electroencephalography (EEG) was measured online with reference to the left mastoid, with a ground electrode on the medial frontal aspect, and later was analyzed offline with re-reference to an average of the left and right mastoids. EEG was analyzed using the SCAN 4.5 software (Compumedics Neuroscan, Texas, United States) and Matlab using the ERPLAB toolbox (Lopez-Calderon and Luck, 2014). The EEG was digitally filtered at 0.1-30 Hz bandpass. Eye movements were corrected with an ocular artifact correction algorithm (Gratton et al., 1983). Artifacts with amplitudes exceeding ±75 µV were removed from analyses. ERPs were time-locked to the onset of the last word of the sentence and were obtained by stimulus-locked averaging of the EEG recorded in each condition. Epochs were 1,000 ms in length with a 200 ms pre-stimulus baseline. The resulting amplitudes of N400 and LPC were entered into 3 condition × 3 region (frontal F3, Fz, F4, central C3, Cz, C4, parietal P3, Pz, P4) × 3 hemisphere (left F3, C3, P3, midline Fz, Cz, Pz, right F4, C4, P4) three-way ANOVAs for repeated measures. All ANOVA results were Greenhouse-Geisser corrected if assumption of sphericity was violated, and post-hoc multiple comparisons were carried out using Bonferroni-adjusted corrections.

Behavioral Results
A repeated-measures ANOVA revealed significant effects of condition for reaction time F(2,32) = 5.32, p = 0.01, η p 2 = 0.25. Pairwise comparisons showed that the reaction time of scientific metaphors was significantly longer than that of literal sentences (p = 0.005). There was neither significant difference between conventional metaphors and literal sentences nor between the two metaphorical conditions (p-values > 0.1). For accuracy rates, a main effect between conditions was found [F(2,32) = 31.12, p < 0.001, η p 2 = 0.49]. Pairwise comparisons showed that the accuracy rate of scientific metaphors was significantly lower than that of conventional metaphors and literal sentences (p-values < 0.01). There was no significant difference between conventional metaphors and literal sentences (p = 0.569).

Electrophysiological Results
According to the Grand average ERP waveforms recorded at the nine chosen electrodes (see Figure 2), there was a sizable negative deflection at about 400 ms, identified as N400, and a later positive deflection appeared from 550 to 800 ms, identified as LPC.

Discussion
Consistent with our predictions, scientific metaphors elicited more negative N400 readings than conventional metaphors. The processing of scientific metaphors involves the conceptual integration between scientific-target and daily-source, while the processing of conventional metaphors involves the conceptual integration of daily-target and daily-source, resulting in the contextual complexity found in scientific metaphors. Compared with conventional metaphors, when processing scientific metaphors, it might be more difficult to search and retrieve stored conceptual knowledge due to the longer distance between the target and source domains (Tang et al., 2017a,b). Meanwhile, larger N400s elicited by scientific metaphors might also indicate the modulation of concreteness. The scientific targets of scientific metaphors were more abstract than the daily targets of conventional metaphors, contributing to the increased negativities found (Forgács et al., 2015).
Consistent with our predictions, both scientific metaphors and conventional metaphors elicited a late negativity partly overlapping in space and time with the LPC (Arzouan et al., 2007a,b;Zucker and Mudrik, 2019). The higher amplitude of scientific metaphors might be caused by the late inference of scientific metaphors from the daily source domain to the scientific target domain in order to understand the related scientific knowledge (Tang et al., 2017a,b). Compared with conventional metaphors, the late stage of scientific metaphor processing involves deeper secondary semantic integration processes. Moreover, as in novel metaphors, the late processing of scientific metaphors is more taxing on working memory (Steinhauer et al., 2010) and more difficult in semantic integration (Zhao et al., 2011;Goldstein et al., 2012;Rutter et al., 2012).

EXPERIMENT 2 (ENGLISH L2)
Method Participants Participants of Experiment 2 were selected from undergraduates with similar ages, language background, and English proficiency as those of Experiment 1. Twenty participants took part in the ERP experiment. As in Experiment 1, the number of subjects included in our final statistical analysis was 17 (8 men, 9 women, average age 21.1 ± 6.54 years).

Stimuli
The English stimuli of Experiment 2 were the English counterparts of the Chinese stimuli used in Experiment 1 FIGURE 3 | Topographic plots of the two kinds of Chinese metaphors subtracting the literal from the metaphoric in the 300-500 and 550-800 ms time windows.
(see Table 2). The English stimuli followed the "A IS B" structure similar to the structure of the Chinese equivalents. As English is a L2 for the subjects, prior to the neurophysiological study, the English stimuli were tested for familiarity by 40 raters who did not participate in the ERP experiment. Before the test, all raters read a pool of English scientific terms used in the scientific sentences. During the test, the raters were asked to decide whether each expression was familiar or not on a 1-5 scale (1 = not familiar, 5 = highly familiar). A total of 120 English stimuli (40 for each category) with a familiarity higher than 3 were chosen for the ERP experiment. A repeated-measures ANOVA revealed a significant main effect of condition [F(2,78) = 39.72, p < 0.001, η p 2 = 0.51]. The familiarity of scientific metaphors was significantly lower than that of conventional metaphors and literal sentences (p-values < 0.001), while no significant difference was found between the familiarity of conventional metaphors and literal sentences (p = 0.225).

EEG Recording and Data Analysis
Same as Experiment 1.

Behavioral Performance
Diverging from the result of Experiment 1, the main effect of condition for reaction time was not found to be significant (p = 0.48). A repeated-measures ANOVA performed on

Scientific metaphors A charge is flow.
A conductor is a tunnel.
A mitochondrion is a code.
A virus is a killer.
Sound is wave.

Conventional metaphors A book is a friend.
Hangzhou is heaven.
Language is a bridge.
Nature is a doctor.
History is a mirror.

Literal expressions A professor is a scholar.
A rose is a plant.
Beijing is a city.
Running is a sport.
Painting is art.
the accuracy rates yielded significant effects of condition [F(2,32) = 14.73, p < 0.001, η p 2 = 0.48]. The difference between the accuracy rates of two metaphorical conditions was not significant (p = 0.775), but the accuracy rates of metaphorical conditions were significantly lower than that of the literal condition (p-values < 0.001).

Electrophysiological Results
According to the Grand average ERP waveforms recorded at the nine chosen electrodes (see Figure 5), there was a sizable negative deflection at about 400 ms (identified as N400) and a later positive deflection from 550 to 800 ms (identified as LPC).
In contrast to Chinese metaphor processing, the differences between English scientific metaphors and conventional metaphors were significant at the frontal [t (16)   Late Negativity. Analogous to Chinese metaphor processing, English (L2) scientific metaphors and conventional metaphors also elicited a late negativity in the LPC time window (see Comparative Results of Experiments 1 and 2 300-500 ms (N400) In comparing the results manifested by Chinese and English scientific metaphors (see Figure 7), a condition × region × hemisphere ANOVA showed that the main effect of condition was not significant [F(1,16) = 1.79, p = 0.46, η p 2 = 0.04], but there were marginally significant

Discussion
Taken together, reduced LPC amplitudes for all three English conditions were maximal over the parietal region, especially over the right parietal region, suggesting that the meanings of both L2 metaphoric and literal sentences are integrated with increased cognitive effort. This result might indicate that late speakers who master two languages asymmetrically are less sensitive to levels of conventionality of metaphoric meanings at the later stage of metaphoric language processing (Jankowiak et al., 2017). Moreover, LPCs are typically considered to reflect the depth of syntactic processing. Compared with L2 processing, the understanding of L1 might involve more syntactic analysis (Van Der Meij et al., 2011).

GENERAL DISCUSSION
The first aim of the current study was to observe brain responses to scientific metaphoric, conventional metaphoric, and literal sentences in Chinese (L1) and English (L2). In line with what was hypothesized, in both the Chinese and English experiments, we observed general between-condition differences, with scientific metaphors eliciting higher N400s than conventional metaphors. However, in the English experiment, more significant effects were found in larger regions over frontal, central, and parietal regions, with a slight right hemisphere bias. Importantly, this finding accords with the behavioral results drawn from the Chinese experiment, which showed lower accuracy rates and longer reaction time for scientific metaphors than with conventional metaphors. This supports the graded salience hypothesis for metaphorical expression. The unique complexity and abstraction of scientific language reduce the explicitness of language expression and have an impact on semantic integration in the later stage of processing. The longer distance between the scientific target domain and the daily source domain for scientific metaphor comprehension might increase cognitive effort in conceptual integration (Tang et al., 2017a,b). According to the Career of Metaphor Model (Bowdle and Gentner, 2005), the processing mechanism of metaphors is regulated by their familiarity. The understanding of metaphors with low familiarity requires the construction of metaphorical meaning through lexical semantic processes, while the understanding of metaphors with high familiarity is mainly carried out through semantic retrieval (Coulson and Van Petten, 2002;Lai and Curran, 2013). In addition, compared with literal expressions, conventional metaphors elicited a larger N400 response, which might indicate that despite being frequently used with high familiarity, conventional metaphors require more resourceintensive mappings between concepts than literal expressions. In both Chinese and English experiments, LPC amplitudes evoked by scientific metaphors were smaller than those elicited by conventional metaphors and literal sentences. Lower LPC amplitudes for scientific metaphors might be caused by a sustained negativity, indexing the integration process of conceptually taxing meanings. Another explanation could be due to reprocessing operations after an initial failure in meaning interpretation (Ruchkin et al., 1988;Rataj, 2014) or alternatively extra working memory load for complex semantic processing (Ruchkin et al., 1988;Anderson et al., 1996;Oberauer et al., 2001;Jiang et al., 2009). According to some monolingual research on metaphors, novel metaphors elicited larger late negativity amplitudes than conventional metaphors, which was interpreted as the continuation of information retrieval or access to the non-literal route when understanding novel metaphors (Arzouan et al., 2007a), and as the ongoing difficulty of meaning integration indexed as the continuation of the N400 effect (Rutter et al., 2012). The larger late negativity of scientific metaphors might further indicate more difficult reintegration of the two domains, especially when the knowledge inference is involved at the later stage of scientific metaphor processing.
Second, the current study aimed to observe whether language nativeness modulates meaning integration mechanisms of scientific metaphors, to which the N400 response is sensitive. More specifically speaking, we were interested in whether L2 scientific metaphors would elicit similar N400s as L1 equivalents for late unbalanced bilingual speakers.
Between-language effects were found with English scientific metaphors eliciting more negative N400s than Chinese ones at the parietal region. Such enhanced N400 response to L2 relative to L1 stimuli is contrary to the results of some bilingual research (Proverbio et al., 2002;Moreno et al., 2008;Midgley et al., 2009;Newman et al., 2012;Heidlmayr et al., 2015). Within the memory system, weaker semantic interconnectivity for L2 compared with L1 words might explain the increased N400 amplitude (Midgley et al., 2009). From the opposite perspective, within the semantic network, larger interconnectivity for L1 words might be linked to the N400 L1/L2 effect (increased N400 amplitudes for L1 relative to L2 words). However, for scientific words, the picture might be different. Compared with conventional words, the semantic connectivity of scientific words might be quite weak in both native and non-native languages. This conjecture would seem to be supported by the behavioral results of the present English experiment. Such weak interconnectivity for scientific words might evoke similar activity in long-term memory in both languages for information retrieval. Unlike the daily words used in conventional metaphors, the scientific terms used in scientific metaphors are even less frequently used, which might result in the much lower familiarity for L2 relative to L1 scientific words. Therefore, the semantic processing of second language scientific terms is more challenging than that of Chinese ones. Moreover, for ordinary L2 speakers (Chang and Wang, 2016), the processing of L2 vocabulary often requires a greater degree of suppression of native vocabulary (Heidlmayr et al., 2015;Wu and Thierry, 2017), which further leads to difficulty in processing L2 words, especially scientific terms.
In addition, at the frontal, central, and parietal regions of the right hemisphere, English scientific metaphors elicited higher N400s than English conventional metaphors, indicating the special role of the right hemisphere in second language processing (Van Der Meij et al., 2011), which supports the Fine-Coarse Semantic Coding Theory (Mashal et al., 2015). Meanwhile, at the left parietal region, there was a marginally significant difference between the two metaphorical sentences, probably showing that the left hemisphere is also involved in understanding L2 scientific metaphors (Kim et al., 2017;Segal and Gollan, 2018).
Third, the current study aimed to observe the modulation of language nativeness for cognitive mechanisms involved at the later stage of meaning reintegration, which the LPC is sensitive to. In line with our predictions, within the LPC time frame, at the parietal region, we observed between-language differences with a slight right hemisphere bias, with smaller LPC responses evoked by the three English conditions than their Chinese counterparts. Reduced LPC amplitudes for scientific and conventional metaphors in the non-native language suggest more demanding cognitive effort to integrate the meaning of both novel and familiar metaphors in an L2 context. This result might indicate that late speakers who master two languages asymmetrically are less sensitive to the levels of conventionality of metaphoric meanings at the later stage of metaphoric language processing.
Inconsistent with the processing of Chinese scientific metaphors, English scientific metaphors only elicited lower LPCs than English conventional metaphors at the parietal region. That is to say, the LPC distribution of L1 scientific metaphors covered a larger area than that of L2 scientific metaphors (Jankowiak et al., 2017;Segal and Gollan, 2018), probably because it might be quite difficult for late L2 learners to reach a similar processing depth as displayed by the native speakers (Newman et al., 2012). In addition, both the left and right hemispheres are involved in the processing of L2 scientific metaphors (Chen et al., 2013), and the right parietal region might play a particularly important role.

CONCLUSION
Through a comparative analysis of the ERP components elicited by scientific metaphors in English and Chinese, this study examined brain responses to scientific metaphors in L2. It was found that nativeness modulates the cognitive cost for semantic integration at an early stage and for semantic reintegration and knowledge inference at a later period, supporting the Career of Metaphor Model and the Graded Salience Hypothesis.
In addition, the scalp distributions of the N400s and the late component elicited by scientific metaphors in L2 reinforce the essential role of the parietal region (especially the right parietal region) in processing L2, supporting the Fine-Coarse Semantic Coding Theory. More abundant types of stimuli could be added to subsequent follow-up experiments for more comparative analysis so as to continuously verify and improve existing studies.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
The studies involving human participants were reviewed and approved by Ethics Committee in Shaanxi Normal University.
The patients/participants provided their written informed consent to participate in this study.

AUTHOR CONTRIBUTIONS
XT and WR contributed to conception and design of the study. XT, MH, YH, and SH performed the data collection. LS, MH, and PY performed the analysis. XT wrote the first draft of the manuscript. All authors contributed to manuscript revision, read, and approved the submitted version.