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Preservation of lettuce necrotic yellows and some other plant viruses by dehydration with silica gel.

Lettuce necrotic yellows virus (LNYV) is very unstable in vitro (Crowley, 1967) and is therefore difficult to store, even by dehydrating virus-infected leaf tissue, a technique which has been particularly successful for the long-term storage of many plant viruses (McKinney & Silber, 1968). Hunt, Gourevitch & Lein (1958) and Perkins (1962) described ways of preserving microbial stock cultures by dehydration with anhydrous silica gel, and these methods have been applied to a range of microorganisms (see Grivell & Jackson, 1969).
In this paper we report that LNYV has been preserved for several years by the technique described by Perkins (1962) for storing fungi, in which a suspension of the fungal spores is mixed with skim-milk and then with anhydrous granular silica gel. Tomato spotted wilt (TSWV), cucumber mosaic (CMV) and cauliflower mosaic (CIMV) viruses have also been stored by this method and later recovered.