{'fraction_non_alphanumeric': 0.017396907216494846, 'fraction_numerical': 0.017396907216494846, 'mean_word_length': 5.781659388646288, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Biodegradation and biosorption of Reactive Red 120 dye by immobilized Pseudomonas guariconensis: Kinetic and toxicity study

Reactive dyes are pernicious pollutants in textile effluent, which are to be treated passably before discharging into the environment. In the present study, a potential dye degrading bacterial strain Pseudomonas guariconensis was isolated from paddy rhizosphere and was characterized by 16S rRNA gene sequencing. The biodegradation ability of the strain was evaluated by time‐based study with immobilized bacterial cells in calcium alginate biocarrier matrix and also with free cells. The results indicated that the strain exhibited maximum degradation of 91% when immobilized in the biocarrier matrix. The enzymatic study revealed the production of oxidoreductase enzymes. The degraded products were identified as 2‐amino‐3‐phenylpropanoic acid and benzoquinone by gas chromatography–mass spectroscopy (GC‐MS) analysis, and a degradative pathway was derived based on the enzymatic profile. A packed bed column was designed using P. guariconensis VITSAJ5 immobilized in calcium alginate beads as a biosorbent for the removal of Reactive Red 120. The immobilized bacterial cells exhibited 87% uptake of RR120, whereas the nonimmobilized bacterial cells exhibited a maximum uptake of 37%. The phytotoxicity analysis by seed germination assay revealed an enhanced plumule and radicle length, indicating the nontoxic byproducts after the treatment of Reactive Red 120 by VITSAJ5 compared to the untreated Reactive Red 120 solution.