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Changes on Three Neurotrophic Factors Expression in Rats with Spinal Cord Contusion Following Olfactory Ensheathing Cell Transplantation

Objective To investigate the expression of three neurotrophic factors(brain-derived neurotrophic factor,ciliary neurotrophic factor,nerve growth factor)in rats with spinal cord contusion following olfactory ensheathing cell transplantation.Methods The OECs were dissociated from the olfactory bulbs of the adult GFP rat,primarily cultured.Forty clean adult Sprague Dawley(SD)rats were randomly divided into OECs transplantation group and control group(DMEM transplantation),two groups were subdivided into four groups,with the rats sacrificed 1,7,14 and 21 days after the operations(day of postoperation,DPO),respectively.The spinal cords of all the rats were completely contused at T10 by NYU with 10g~25 mm.The Basso,Beattie and Bresnahan locomotor testing scales(BBB scores)were used to evaluate the locomotor function of hindlimbs of the rats.The expressions of three neurotrophic factors in the spinal cords were detected with immunohistochemistry technique.Results The BBB scores increased gradually with the time after the spinal cord injury.But the hindlimb locomotor function did not improve significantly.Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter,delay cells reduction in spinal white matter,and accelerate the medullary sheath repair and regeneration.The expression of three neurotrophic factors(BDNF、CNTF、NGF)in spinal cord tissue after transplantation were obvious increased than that of DMEM/Ham’s F-12 group(P0.01).Conclusion OECs transplantation can partly improve the pathology morphous of injured spinal cord,facilitate the expression of three neurotrophic factors.