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Study of the mechanisms involved in hydroxocobalamin interference with determination of some biochemical parameters.

Hydroxocobalamin (OHCo), a red pigment used as an antidote in cyanide poisoning, interferes with determination of some biochemical parameters. Plasma pools were spiked with two concentrations of OHCo and eight parameters (CK, SGOT, SGPT, ALP, lactic acid, creatinine, glucose, bilirubin) were assayed using Dimension and Aca III automated analyzers (Du Pont Instruments). Two parameters were affected by the presence of OHCo: CK and bilirubin. This study documents the type of interferences, spectral or chemical, and its probable causes.