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HPTLC QUANTIFICATION OF POLYPHENOLIC ACIDS AND ANTIOXIDANT ACTIVITY OF POLYGONUM HYDROPIPER L. SPECIES FROM ROMANIAN FLORA

For the flowering aerial parts of Polygonum hydropiper L. species, harvested from Romanian flora, four polyphenolic acids were separated and quantified using HPTLC-UV densitometry, with ESI-MS confirmation. Chlorogenic acid was found in the highest amount, followed by ferulic acid, p-coumaric acid and caffeic acid. The content of total polyphenols and caffeic acid derivatives was determined using spectrophotometric methods. In vitro antioxidant activity was evaluated using DPPH assay. Our studies bring new data regarding the phytochemical profile of P. hydropiper, as a natural source of polyphenols with antioxidant properties.