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[Histochemical estimation of zinc accumulation in hepato-pancreatic gland cells of the snail Lymnaea stagnalis (L.)after subacute poisoning with zinc acetate considering infection with Digenea larvae].

Accumulation of zinc in epithelial cells of the digestive gland tubules of the fresh-water snail Lymnaea stagnalis after subacute intoxication with zinc acetate (strong increasing doses of the metal in short period of time) was histochemically examined using dithizone. Large zinc granules observed in medial and basal parts of calcium cells permitted to conclude that this metal is bound in cytoplasm mainly on granules built of calcium and magnesium phosphate. There were no smaller concentrations of zinc, that would be indicative for metal binding with cytoplasmatic metallothionein-like proteins. In calcium cells of the digestive gland tubules of snails infected with Digenea larvae accumulation of zinc was stronger, that would be an aspect of compensatory reaction.