{'fraction_non_alphanumeric': 0.027835051546391754, 'fraction_numerical': 0.007216494845360825, 'mean_word_length': 4.776785714285714, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance


 Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease related to infertility in women. IVF-ET is the effective way to treat infertility in PCOS patients. But poor oocytes quality of PCOS patients results in low pregnancy rate and the underlying mechanism is unknown. Klotho, an anti-aging factor, was reported up-regulated in PCOS and related to excessive androgens. However, the role of Klotho in poor oocytes quality of PCOS has not been clarified. Method: We enrolled 72 PCOS and 70 healthy participants who underwent IVF-ET and the follicular fluid was collected. In addition, granulosa cells from 15 patients underwent IVF-ET were collected. The level Klotho in follicular fluid was tested by ELISA. The mRNA level of Caspase-3, Caspase-9, IL-6, TNF, PCNA and GDF9 in granulosa cell were detected by qRT-PCR.Results: Compared with the control group, the level of Klotho, Caspase-3, Caspase-9, IL-6 and TNF in PCOS group was significantly higher, especially in the group of PCOS with hyperandrogenism, while the level of PCNA and GDF9 was down-regulated. Correlation analysis shown that level of Klotho in follicular fluid of PCOS group was negatively correlated with the number of mature oocytes. Similarly, we divided the PCOS group into two groups according to the median of Klotho level and found that the number of mature oocytes is significantly lower in the group of high level of Klotho. In addition, the Klotho level in follicular fluid was also positively correlated with serum testosterone, LH, LH/FSH, menstrual cycle and number of total antral follicles in the group of PCOS. Conclusions: These data suggested that Klotho may act as a new biomarker evaluating the quality of oocytes in patients with PCOS, indicating the severity of PCOS and the outcome of IVF in PCOS patients.