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Analysis of riboflavin in commercial multivitamin preparations by high-speed liquid chromatography.

A high-speed liquid chromatographic procedure for analysis of riboflavin in multivitamin preparations was developed and evaluated. The method utilizes 2, 2′-diphenic acid as the internal standard and 254-nm detection of separated constituents. This procedure was compared with that of the USP with respect to accuracy in the analysis of riboflavin in the presence of its photolytic degradation products. In addition, the procedure was applied to the analysis of several commercial multivitamin products containing riboflavin, with reproducible results ranging from 27.23 to 103.45% of the labeled amount of riboflavin.