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Titer and Prevalence of Fusobacterium Nucleatum in Histological Section of Human Oral Epithelial Cells in Patients with Chronic Periodontitis

Introduction: Anaerobic bacteria in the oral microbial flora are involved in many infections of the oral cavity and surrounding structures. Fusobacterium nucleatum is believed to differ from other bacteria in its interaction with oral mucosal epithelial cells. The aim of this study was to determine the prevalence of Fusobacterium nucleotum in the epithelial cells of the oral mucosa of patients with periodontitis compared with healthy individuals. 
Materials and Methods: In this case-control study, Realtime-PCR technique used to detect the Fusobacterium nucleatum in gingival samples of 15 patients with chronic periodontitis and 15 individuals with clinically healthy gingival specimens. The results analyzed by Fisher's exact and Independent t-tests with a significance level of less than 0.05. 
Results: The Fisher's exact test showed that the F.nucleatum in patients with periodontitis was higher than the healthy population. Also Independent t-test showed that average titer of bacteria in patients with Periodontitis was significantly more than people without gingival inflammation (p value < 0.001). 
Conclusion: There is a statistically significant relationship between periodontitis and the presence of F.nucleatum in epithelial cells of oral mucusa.