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Two Viruses from the Lucké Tumor Isolated in a Frog Pronephric Cell Line 1

Summary The first established frog pronephric cell line was exposed to tumor cell fractions of the oncogenic (nuclear inclusion body) phase of the Lucké renal adenocarcinoma. A mixed infection of the herpes virus and adeno-like virus was obtained. When tested by embryonic bioassay, the original tumor fractions and the first passages of the viruses in tissue culture produced subdermal hemorrhage, turgid edema and total deaths in 3-6 weeks. In subsequent passages the adeno-like virus appeared to abort and the herpes virus predominated. Bioassay of these and later passages produced no trauma within 3 months.