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Francisella tularensis live vaccine strain: Proteomic analysis of membrane proteins enriched fraction

Proteome analysis of Gram‐negative facultative intracellular pathogen Francisella tularensis (F. tularensis) live vaccine strain has been performed only on whole‐cell extracts so far. This is the first study dealing with the analysis of the membrane subproteome of this microorganism. A fraction enriched in membrane proteins obtained by carbonate extraction was separated using two‐dimensional electrophoresis and all visualized spots were identified by mass spectrometry. The reference map is the basis for further comparative analyses of virulent and non‐virulent F. tularensis strains.