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One base pair resolution histone H1-nucleosomal DNA interaction mapping and 3D organization of H1 containing nucleosome

In this work we have been able to dissect how histone H1 interacts with the nucleosomal DNA and to understand how this interaction leads to the spatial organization of the nucleosomal templates. We have solved this long-stayed problem in the field thanks to the use of: (i) physiologically relevant linker histone chaperone NAP-1 assisted deposition of histone H1, (ii) 601 DNA sequence for reconstitution a strongly positioned nucleosomes, (iii) a combination of electron cryo-microscopy with OH0 footprinting techniques and, (iv) Coarse-grain DNA mechanics. The one base pair resolution mapping by OH0 footprinting showed that the globular domain of histone H1 (GH1) interacts, through the minor groove of DNA, with 10 bp localized symmetrically to the nucleosomal dyad. In addition, GH1 organizes nearly one helical turn of DNA from both linkers of the nucleosome. A row of seven AA (120-127) of the COOH-terminus of histone H1 was required for the formation of the stem structure of the linker.