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The distribution of collagenous connective tissue in rat ovarian follicles.

The layers of thecah tissue in the ovarian follicles of mammals normally contain collagen. During the past year, it has been reported that the follicle wall of the rat uterus is an exception. The present report demonstrates that the rat follicle does contain collagen in abundance. This thecal collagen is very conspicuous when the tissue has been stained with phosphotungstic acid. However, collagen is not obvious in ultrathin sections that have been stained with the lead solutions which are commonly used in electron microscopy. These results maintain the current hypothesis that the mechanism of ovulation requires the disruption of collagenous connective tissue in the follicle wall.