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REVERSED PHASE HPLC OF OXYETHYLATES OF N-METHYLANILINE AND N-ETHYLANILINE

The conditions were selected for the reversed phase HPLC separation (phosphate buffer pH = 7.4 / methanol) of N-oxyethylates of N-methylaniline and N-ethylaniline on C18 columns, depending on the oxyethylene chain length. The use of the “mixed-mode” C18/anion column provided the possibility for simultaneous separation of oxyethylates, as well as, N,N-dimethylaniline and N,N-diethylaniline. UV spectra were taken for the compounds studied in order to find the optimum wavelengths for the detection procedure.