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Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier

Aim: Attempts were made to immobilize laccase from Panus conchatus.  Methods and Results: The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) activated by N‐hydroxysuccinimide (N‐HSI) in aqueous solution at different pHs, temperatures, and with different reaction times. An optimum condition for laccase immobilization is at pH 3·2, 40 °C and 12 h, respectively. Immobilization of laccase increased optimal pH for reaction with 2, 2′‐azinobis (3‐ethylbenzthiazoline‐6‐solfonate) (ABTS) and pH stability. Immobilized laccase proved to be reacted consecutively 17 times with only a 50% decrease on activity and be used in removal of 2,4,6‐trichlorophenol (TCP).  Conclusions: It was possible to immobilize the laccase on carboxylated polyvinyl alcohol by activation with N‐hydroxysuccinimide in HAc‐NaAc buffer. The immobilized laccase is both stable and reusable.  Significance and Impact of the Study: The results indicate that this immobilized laccase can be used in the removal of poisonous effluent from pulp bleaching mills.