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A probabilistic approach for characterizing the marking system of multiplex sequencing in ABI SOLiD platform

High-Throughput Sequencers such as Illumina and ABI SOLiD, generate large quantities of data, typically above 10 Gigabytes of text files. These platforms enable multiplex sequencing, that is, the sequencing of multiple samples in a single run, through a marking system. This requires a computational process for separation the data generated, which contains the mixture of all samples in a single output. It is necessary that the quality of the marking system is evaluated to ensure the reliability of this separation. This work proposes measures to characterize the marking system obtained from SOLiD sequencing. In fact, measures presented are proven to be sufficient to describe the sequencing and hence guide the process of filtering the data and the analysis of the sequencing protocol.