{'fraction_non_alphanumeric': 0.03836817790306162, 'fraction_numerical': 0.025056769242815753, 'mean_word_length': 4.998747455769532, 'pattern_counts': {'":': 0, '<': 3, '<?xml version=': 0, '>': 1, 'https://': 0, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The influence of 5-HTTLPR transporter genotype on amygdala-subgenual anterior cingulate cortex connectivity in autism spectrum disorder

Social deficits in autism spectrum disorder (ASD) are linked to amygdala functioning and functional connection between the amygdala and subgenual anterior cingulate cortex (sACC) is involved in the modulation of amygdala activity. Impairments in behavioral symptoms and amygdala activation and connectivity with the sACC seem to vary by serotonin transporter-linked polymorphic region (5-HTTLPR) variant genotype in diverse populations. The current preliminary investigation examines whether amygdala-sACC connectivity differs by 5-HTTLPR genotype and relates to social functioning in ASD. A sample of 108 children and adolescents (44 ASD) completed an fMRI face-processing task. Youth with ASD and low expressing 5-HTTLPR genotypes showed significantly greater connectivity than youth with ASD and higher expressing genotypes as well as typically developing (TD) individuals with both low and higher expressing genotypes, in the comparison of happy vs. baseline faces and happy vs. neutral faces. Moreover, individuals with ASD and higher expressing genotypes exhibit a negative relationship between amygdala-sACC connectivity and social dysfunction. Altered amygdala-sACC coupling based on 5-HTTLPR genotype may help explain some of the heterogeneity in neural and social function observed in ASD. This is the first ASD study to combine genetic polymorphism analyses and functional connectivity in the context of a social task.


Introduction
Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by heterogeneous deficits in social interaction and communication (APA, 2013). Social deficits in ASD are linked to abnormalities in the functioning of the amygdala, a limbic structure involved in processing salient, often emotion-ally charged, stimuli (Baron-Cohen et al., 1999;Critchley et al., 2000;Kleinhans et al., 2009;Monk et al., 2010;Weng et al., 2011). For example, Kleinhans et al. (2009) showed that lower rates of amygdala habituation to emotion are related to more severe social impairment based on social affect subscale scores from the Autism Diagnostic Observation Schedule (ADOS; Lord et al., 2000), and Swartz et al. (2013) found that lower habituation rates are related to greater impairment based on the Social Responsiveness Scale (SRS; Constantino et al., 2003), a more general measure of impairment (Hus et al., 2013). Early fMRI studies on amygdala functioning reported that ASD groups display reduced activation in this region relative to typically developing (TD) groups in response to emotional faces (Baron-Cohen et al., 1999;Critchley et al., 2000;Pierce et al., 2001), whereas more recent ASD studies found amygdala hyperarousal with a shorter stimulus presentation time (  Depiction of face-processing task displays the order and duration of each stage in the procedure. Gender identification stage was the time participants were given to indicate the gender of the person displayed in the stimulus presentation. Intertrial interval ranged from 0 to 6000 ms with intervals of 2000 ms. A total of 60 faces (15 per emotion) were presented. The stimuli used were obtained from the NimStim Set (Tottenham et al., 2009(Tottenham et al., ). et al., 2010Weng et al., 2011;Kliemann et al., 2012). Such discrepant findings have warranted further investigation into the structures modulating amygdala activity.
The subgenual anterior cingulate cortex (sACC) has dense cortical connections to the amygdala, and is involved in amygdala inhibition (McDonald, 1998;Milad and Quirk, 2012;Ray and Zald, 2012), possibly through excitatory signaling to inhibitory regions of the amygdala (Rosenkranz and Grace, 2001). Connectivity between the sACC and the amygdala is implicated in the extinction of negative affect (Milad and Quirk, 2002;Maren and Quirk, 2004) and is linked to various mood disorders (Pezawas et al., 2005;Drevets et al., 2008).
5-HTTLPR is a functional polymorphism found in the promoter region of the gene that encodes the serotonin transporter (SERT), a protein that modulates serotonin function (Hu et al., 2006). The 5-HTTLPR polymorphism directly affects SERT expression and consequently serotonin function in that individuals with the short variant (S) produce less SERT mRNA and protein than individuals with the long (L) variant, thus creating increased levels of serotonin in the synaptic cleft. TD individuals who are carriers of 5-HTTLPR short (S) alleles exhibit increased anxiety related traits (Canli and Lesch, 2007), depressive symptoms (Caspi et al., 2003), as well decreased functional connectivity between the amygdala and the sACC when looking at fearful faces (Pezawas et al., 2005).
Whereas studies have found inconclusive evidence of an association between 5-HTTLPR and susceptibility to ASD (e.g. Cook and Leventhal, 1996;Devlin et al., 2005;Zhong et al., 1999;Ramoz et al., 2006), various reports highlight the effect of genotype on the severity of social dysfunction in ASD, which may be related to amyg-dala functioning and its connectivity with the sACC. For example, Tordjman et al. (2001) found evidence of increased severity in the Combined Social and Communication domain subset of the Autism Diagnostic Interview-Revised (ADI-R; Lord et al., 1994) in participants with the S allele compared to those homozygous for the L allele. Similarly, Brune et al. (2006) found an effect of 5-HTTLPR genotype on non-verbal social interaction severity where participants with S/S or S/L genotypes displayed more severity compared to those with the L/L genotype; individuals with L/L genotypes exhibited increased severity of restricted and repetitive behaviors. There are reports however, pointing to more severe social deficits in participants homozygous for the L allele (Gadow et al., 2013), which contradicts previous data. In sum, 5-HTTLPR seems to affect aspects of social dysfunction in ASD, despite a lack of association between 5-HTTLPR and susceptibility to ASD. 5-HTTLPR can be subdivided into further allelic variants (Nakamura et al., 2000). Short (S) alleles and long (L) alleles with genotypes that contain the G (i.e. S G and L G ) variants are associated with decreased SERT expression compared to individuals with long alleles and genotypes that contain the A variant (i.e. L A ; Hu et al., 2006). Recent studies have grouped S/S, S/L G and L G /L G genotypes as low expressing genotypes of the 5-HTTLPR polymorphism (Gadow et al., 2013;Wiggins et al. 2014a,b). Wiggins et al. (2013) examined the influence of 5-HTTLPR on resting connectivity in ASD and found that youth with the low expressing genotypes exhibited stronger connectivity in the default network than higher expressing genotype groups, whereas the opposite was true for the TD group. In addition, youth with low expressing genotypes show decreased amygdala habituation (i.e., more sustained activation over time) in response to emotional faces (Wiggins et al., 2014a).
In sum, data do not point to 5-HTTLPR as a susceptibility gene for ASD; nevertheless, impairments in behavioral symptoms and differences in brain function vary by genotype within individuals with ASD as well as in TD individuals. Thus, we examined whether amygdala-sACC connectivity differs by genotype and relate to social functioning in ASD. We hypothesized a diagnosis (ASD vs. TD) by 5-HTTLPR genotype (low vs. higher) interaction on amygdala-sACC connectivity to specific emotional faces. We further tested Brain image displays area where differences were observed (peak xyz = −4, 22, −12) in Left BA 25 (small-volume corrected), at a voxelwise threshold of p < 0.05. The difference in connectivity between the ASD low expressing group and the TD low expressing group remained significant after Bonferroni correction for multiple comparisons. Values for graph were extracted from left BA 25, defined structurally by the Wake Forest University Pickatlas (Maldjian et al., 2003).
whether amygdala-sACC connectivity was related to social symptoms within ASD.

Participants
We recruited 187 participants. The final sample consisted of 43 individuals with ASD (Mean age = 13.5, SD = 3.26) and 65 TD individuals (Mean age = 14.7, SD = 3.73), ranging from 8 to 19 years of age. Reasons for data exclusion were: excessive head motion (maximum displacement > 2.25 mm translation or 2.25 • rotation) in any direction compared to the initial position, lack of amygdala or sACC coverage (below 90%), insufficient data after motion censoring (<60% of total time points censored), scoring below our accuracy cutoff (70%) in the gender identification task performed in the scanner, absent or incomplete magnetic resonance imaging (MRI) scan due to discomfort, fMRI technical problems, or because participants did not provide a saliva sample for genotyping (See Supplementary materials Section 1.1 for details). Participants considered for the study did not have metal in their bodies and other physical or neurological conditions that are contrary to the MRI safety guidelines. Preliminary analyses yielded no significant differences in verbal and non-verbal cognitive function tests between diagnosis groups (ASD and TD, low and higher expressing genotypes; see Table 1 in Supplementary materials for additional detail). These data overlap with prior studies from our research group (Swartz et al., 2013(Swartz et al., , 2014Weng et al., 2010Weng et al., , 2011Wiggins et al., 2014a,b).
Individuals with ASD were diagnosed at the University of Michigan Autism and Communication Disorders Center (UMACC). Diagnoses were based on the ADOS, ADI-R, and clinical expertise . TD individuals were recruited using flyers posted in approved posting areas around Ann Arbor, Michigan. The Institutional Review Boards of the University of Michigan Medical School oversaw and approved the methods and procedures conducted by this study. Adult participants and parents of minors signed informed consent forms. Participants under the age of 18 also gave assent.
Participant demographic and behavioral measures were obtained in a data-collection visit prior to their MRI scan. Pubertal development was measured using the Pubertal Development Scale (Petersen et al., 1988). Verbal and non-verbal cognitive function tests were measured using the Peabody Picture Vocabulary Test (Dunn and Dunn, 1997), the Differential Ability Scales (DAS), the Stanford-Binet Intelligence Scales, the Wechsler Intelligence Scale for Children, or the Ravens Progressive Matrices (Raven, 1960). Aside from MRI safety exclusion, we also assessed for developmental, emotional, and behavioral conditions using the Child Depression Inventory (CDI; Kovacs, 1992), Multidimensional Anxiety Scale for Children (MASC; March, 1997), Child Behavior Checklist (CBCL; Achenbach, 1991), and Spence Children's Anxiety Scale (Spence, 1998). Preliminary tests looking at these behavioral measures among diagnosis groups (ASD vs. TD; see Table 1 in Supplementary materials) yielded no significant differences.

5-HTTLPR procedures
Genetic analyses were performed using saliva samples collected with Genotek Oragene DNA kits (DNA Genotek, Kanata, Canada). The first step in the analysis was to differentiate between S and L alleles of 5-HTTLPR. For this, we used polymerase chain reaction (PCR) and agarose gel electrophoresis. To find whether the L allele presented an A or a G SNP, we used Sanger sequencing (Hu et al., 2006). Individuals with ASD and TD individuals were subdivided into low expressing and higher expressing genotype groups. Low expressing genotype groups were composed of individuals with S/S, S/L G and L G /L G genotypes. The higher expressing genotype groups were composed of individuals with medium and high expressing alleles L A /L A , S/L A , and L A /L G genotypes. S and L G alleles were grouped as they have been shown to drive 5-HTTLPR expression nearly equivalently (Hu et al., 2006). These groups were also chosen to address sample-size concerns that stem from sub- Fig. 3. The relationship between ADOS Social Affect scores and Amygdala -sACC connectivity in ASD low and higher expressing genotypes. Brain images displays area (xyz = −8, 22, −12) where the ADOS Social Affect scores showed a negative relationship with amygdala-sACC connectivity (happy vs. baseline) for individuals with ASD and higher expressing genotypes, but not for individuals with ASD and low expressing genotypes. Scatterplot is used for visualization purposes only. Values for graph were extracted from structural left BA 25, defined structurally by the Wake Forest University Pickatlas (Maldjian et al., 2003). dividing by L alleles into smaller groups, similar to past studies (Cicchetti et al., 2007) as well to correspond to the data from our previous studies (Wiggins et al., , 2014a. Fifteen indi-viduals2 with ASD displayed low expressing genotypes and 28 displayed higher expressing genotypes. Within the TD group, 23 individuals displayed low expressing genotypes and 42 displayed higher expressing genotypes. Hardy-Weinberg Equilibrium tests were performed on 5-HTTLPR genotypes (S/S, S/L, L/L) within each group. Hardy-Weinberg equilibrium was not met in the TD group, 2 (1, N = 65) = 5.53, p = 0.02. However, Hardy-Weinberg equilibrium was met in the ASD group, 2 (1, N = 44) = 0.36, p = 0.55. In analyses containing all participants who provided a saliva sample for genotyping, both ASD, 2 (1, N = 89) = 0.0112, p = 0.9156, and TD groups, 2 (1, N = 79) = 3.59, p = 0.0581, met Hardy-Weinberg equilibrium.

Procedural tasks
A face-processing task ( Fig. 1) was presented to the participants inside the scanner. Faces used were part of the NimStim Set (Tottenham et al., 2009). Faces presented were model numbers: 1,7,10,12,15,16,17,20,23,25,30,34,38,40, and 42 of this stimulus set. Each expression was presented 15 times, each time by a different model in a randomized order, for a total of 60 faces. The expressions presented were fearful, happy, neutral, or sad faces. Half of the faces presented were male and half were female. Eight models were European-American, four were African-American, and the remaining three models were Asian-American.
Faces were presented for 250 ms to avoid group differences in attention found in studies where stimuli were presented for longer periods of time (Klin et al., 2002;Pelphrey et al., 2002). We used Eprime 2.0 (Psychological Software Tools, Pittsburgh, PA) to display the stimuli and record the responses. Before every face presentation, a black screen with a white fixation cross was presented for 500 ms. Participants were given 1500 ms after every face presentation to identify the gender of the person in the stimulus via button press. A randomized intertrial interval (ITI) followed the response and ranged from 0 to 6000 ms with intervals of 2000 ms. The ITI represented the implicit baseline. Univariate ANOVAs were used to test for differences in reaction time and accuracy in our face-processing task among the four diagnosis-by-genotype subgroups.
After the scanning procedure, we acquired emotion recognition accuracy data by presenting participants with 120 trials showing the same expressions as in the fMRI task. Each emotion was shown 30 times. Participants were given a laptop equipped with E-Prime and were instructed to indicate what emotion they saw in every trial. Each trial consisted of the same fixation cross screen presented for 500 ms followed by the emotional stimulus for 250 ms. Participants were then given a multiple choice screen where they were asked to indicate whether the emotion they saw was fearful, happy, neutral or sad. A repeated-measures genotype x diagnosis x emotion tested for differences in emotion recognition accuracy.

fMRI data analysis
Imaging data were preprocessed as part of the standard processing procedure at the University of Michigan. K-space outliers greater than two standard deviations from the mean were removed from the raw data and were replaced with the average of the contiguous time points. K-space data were also reconstructed using field map correction to remove magnetic field inhomogeneity distortions. Slice timing differences were corrected for using local sinc interpolation (Oppenheim et al., 1989) with the middle slice as the temporal reference point. Finally, images were realigned and corrected for motion with MCFLIRT in FMRIB Software Library (Jenkinson et al., 2002) using the 10th functional images as reference.
The SPM8 Matlab toolbox (Wellcome Department of Cognitive Neurology, London, UK; http://www.fil.ion.ucl.ac.uk) was used to further preprocess the data. Co-registration to the functional images was performed on the anatomical images. These were then smoothed using an isotropic 8 mm full width at half maximum (FWHM) Gaussian kernel. Individual subject level condition effects were run through the general linear model using SPM. Canonical hemodynamic response function (HRF) and HRF temporal derivative were modeled to each time emotional face stimulus presentation (Friston et al., 1997). A separate regressor was computed for each emotion, yielding 4 regressors of interest at the individual level of analysis. Incorrect responses to the gender of the face stimulus as well as the six motion parameters (x, y, z, roll, pitch, yaw directions) were treated as separate regressors and excluded in this process. To further address head motion, volumes with more than 1 mm framewise displacement were censored. A high pass filter of 128 s was also used to remove the influence of linear drift. Using SPM, we conducted a psychophysiological interaction (PPI) analysis to investigate whether there were differences in connectivity in response to facial stimuli displaying the four chosen emotions (fearful, happy, sad, and neutral). We created a 6 mm sphere around a voxel located in the left amygdala (xyz −30, −6, −14), as the seed region to generate functional connectivity images. The sphere was centered around the left amygdala and not the right because of amygdala regulation interactions found in the left amygdala, between diagnosis (ASD vs. TD) and genotype (low vs. higher) in this same sample (Wiggins et al., 2014a). The mask chosen for small volume correction as sACC was Left Brodmann Area 25 (Left BA 25), defined structurally by Wake Forest University Pickatlas (Maldjian et al., 2003); this area was previously used in human and non-human animal research linking amygdala functioning to sACC connectivity (Swartz et al., 2013;Fisher et al., 2009).
Group analyses were conducted using SPM8 to create statistical models that tested for differences in the patterns of connectivity between the groups. Initial models included accuracy as a covariate to account for variations in attention to the stimuli presented. Analyses were performed for fearful, happy, sad, and neutral each compared to baseline condition, as well as to fearful, happy, and sad each compared to neutral stimuli. We tested for differences in connectivity using a small volume correction in Left BA 25, at a voxelwise threshold of p = 0.05. This small-volume correction searched within BA25 and applied a family-wise error correction based on the size of the left BA 25 mask (Worsley et al., 1996). We then controlled for multiple comparisons of stimuli by setting the voxelwise threshold for significance at p < 0.007 (p = 0.05/7). These analyses compared connectivity between the four diagnosis by genotype groups (ASD low, ASD higher, TD low, and TD higher).
In order to understand how connectivity is related to heterogeneity in social function deficits commonly observed in ASD (Pelphrey et al., 2011), we conducted analyses testing the relationship between amygdala-sACC connectivity and the social subscale of the ADOS. To do so, we entered ADOS Social Affect scores as a covariate in an SPM8 multiple regression analysis. These analyses were conducted within the low and higher expressing 5-HTTLPR genotype groups with ASD, using a small volume correction in Left BA 25, at a voxelwise threshold of p = 0.05.

Behavioral results
Participant ability to identify emotions was tested outside the scanner. There were no group differences in the accuracy to identify each of the emotions presented (Diagnosis × Genotype × Emotion; F(1, 101) = 0.435, p = 0.76). There were no group differences in the accuracy of gender identification for our face-processing task inside the scanner (Diagnosis x Genotype interaction; F(1, 105) = 1.68, p = 0.18). Analyses of reaction time in response to the faceprocessing task showed no significant differences in reaction time. However, the p value suggested a trend towards faster reaction time in the TD Low expressing genotype group relative to the remaining 3 groups, F(1, 103) = 2.61, p = 0.06. To better understand this trend, we conducted an analysis of whether face processing reaction time differed among genotypes. Reaction times were lower in TD individuals with low expressing genotypes compared to all other groups in response to fearful faces, F(3, 103) = 3.16, p = 0.03. There were also trends for lower reaction time to happy (p = 0.078) and neutral faces (p = 0.107) in TD individuals with low expressing genotypes. There were no significant differences among the four groups in reaction time to sad faces (p = 0.257). Our sample differed in age, F(1, 105) = 4.73, p = 0.04 and pubertal development (F(1, 105) = 4.08 p = 0.01) among the four diagnosis-by-genotype subgroups (See Table 1 in Supplementary materials for means). The effects of age differences on connectivity are discussed in Section 3.4.

Connectivity results
A significant diagnosis-by-genotype interaction on amygdala -sACC connectivity was observed in the contrast of happy vs. baseline after controlling for multiple comparisons of emotion, xyz = −4, 22, −12, t(103) = 3.67, p = 0.006, Fig. 2. Specifically, individuals with ASD and low expressing 5-HTTLPR genotypes showed significantly greater amygdala-sACC connectivity than ASD higher expressing genotype group and both TD 5-HTTLPR genotype groups. Individuals with ASD and low expressing 5-HTTLPR genotypes yielded significantly greater connectivity than the three comparison groups in response to the happy vs. neutral contrast, xyz = −6, 24, −12, t(103) = 3.59, p = 0.008. This difference did not survive Bonferroni correction at a voxelwise threshold of p = 0.007. Sad, fearful, and neutral vs. baseline contrasts; and sad, fearful vs. neutral contrasts, did not show diagnosis-by-genotype connectivity interactions in Left BA 25.

Connectivity differences and behavior
A significant negative relationship between amygdala-sACC connectivity in the happy vs. baseline contrast and scores on the ADOS social affect subscale was found in the ASD higher expressing genotype group, t(27) = 3.31, p = 0.038; voxelwise threshold: p = 0.05, but not in the ASD low expressing genotype group, t(12) = 0.79, p = 0.81; voxelwise threshold raised to p = 0.5 to report values; Fig. 3. Weng et al. (2011) showed that individuals with ASD yielded increased amygdala activation using this same task. To rule out the possibility that the present result was observed due to amygdala activity differences and not connectivity between the amygdala and the sACC, we included amygdala activation values extracted from the left amygdala (defined structurally by WFU pickatlas) as a covariate. Connectivity continued to be significantly greater for individuals with ASD and low expressing genotype in response to happy versus baseline faces, xyz = −4, 22, −12, t(102) = 3.60, p = 0.008, and neutral faces, xyz = −6, 24, −12, t(102) = 3.53, p = 0.01.

Post-hoc results
In separate analyses, we tested the influence of age on connectivity, as the average age was found to be significantly different between the four ASD/TD 5-HTTLPR genotype groups. Without the variance associated to age, connectivity continued to be significantly greater for individuals with ASD and low expressing genotype in response to happy versus baseline faces, xyz = −4, 22, −12, t(102) = 3.61, p = 0.005 and to happy versus neutral faces, xyz = −6, 24, −12, t(102) = 3.69, p = 0.006.
Finally, there was a substantial difference in the percentage of Caucasian participants between the four groups (see Supplementary materials: Table 3). Given differences in processing other-race faces (Golby et al., 2001), we conducted a Caucasian only test (n = 89) to see if the results remained. A diagnosis by genotype interaction where individuals with ASD and low expressing genotypes yielded higher connectivity to happy faces was observed using a 6 mm region of interest around the peak voxel of our main result, xyz = −6, 24, −14, t(83) = 3.07, p = 0.012.

Discussion
The present preliminary investigation examined whether 5-HTTLPR genotype may help to explain heterogeneity in terms of neural function within ASD by examining patterns of amygdala-sACC connectivity in response to specific faces between 5-HTTLPR genotype groups in individuals with ASD and TD individuals. We hypothesized that there would be a diagnosis (ASD vs. TD) by 5-HTTLPR genotype (low vs. higher) interaction on amygdala-sACC connectivity to specific emotional faces. Supporting our hypothesis, individuals in the low expressing genotype ASD group showed significantly greater connectivity than the ASD higher expressing genotype group, as well as both TD groups, in response to happy faces relative to a baseline, and to happy relative to neutral faces.
We further tested whether amygdala-sACC connectivity to happy faces was related to symptoms of social dysfunction within the two ASD 5-HTTLPR groups. We found that ADOS social affect scores negatively predicted amygdala-sACC connectivity to happy faces in the ASD higher expressing genotype group but this relationship was not present in the low expressing ASD group. That is, in individuals with ASD and higher expressing 5-HTTLPR genotypes, less severe social dysfunction is related to more connectivity. On the other hand, connectivity in the ASD low expressing genotype group does not significantly predict changes in SRS social motivation scores, despite the presence of dysfunction in social symptoms.
If replicated in a larger sample, the relationship between lower levels of social dysfunction and higher connectivity in participants with ASD and higher expressing genotypes points to the possibility that connectivity between the amygdala and the sACC, at rates comparable to TD individuals, may help modulate social function when individuals view happy faces (e.g. positive social interactions). This possibility is supported by studies showing that the areas of the ventral prefrontal cortex modulate amygdala activity (Milad and Quirk, 2012;Ray and Zald, 2012), and that greater connectivity between the amygdala and the sACC is related to greater amygdala habituation to sad faces in controls (Swartz et al., 2013). Contrastingly, individuals with ASD and low expressing genotypes showed hyperconnectivity at all levels of social dysfunction. We speculate that functional connectivity at abnormally high rates may impede a modulatory relationship between connectivity and social dysfunction.
Moreover, these findings add to the body of literature documenting that individuals with ASD and low expressing 5-HTTLPR genotypes exhibit marked differences in brain function in comparison to other groups. Using a sample overlapping with that of the present study, Wiggins and colleagues found that participants with ASD and low expressing genotypes displayed decreased amygdala habituation (i.e., more sustained activation over time) during the same face-processing task as the present study (2014a) and stronger age-related increases in default network connectivity values during rest  relative to those with ASD and higher expressing genotypes as well as controls.
There are many complex steps between the functioning of a gene variant and brain function. Therefore, it is currently not possible to identify the neurochemical mechanisms that affect amygdala-sACC connectivity throughout development. Furthermore, the reason for differential effects of 5-HTTLPR genotypes between TD individuals and those with ASD is yet unknown. Nevertheless, one possible mechanism may be that amygdala-sACC connections are affected in individuals with low expressing 5-HTTLPR genotypes through an alteration in SERT early in development, possibly affecting emotion regulation. Disruptions in SERT function during central nervous system development affect adult emotional responses to novelty and stress in mice (Ansorge et al., 2004;Rebello et al., 2014) as well as the development of pyramidal neurons involved in inhibition and excitability of prelimbic and infralimbic neurons in the mice homolog of the medial prefrontal cortex (Rebello et al., 2014). It may be that the connection between amygdala and sACC is affected by abnormal serotonin levels in individuals with ASD and low expressing genotypes, as individuals with this variant show abnormal serotonin levels (Canli and Lesch, 2007) and 30% of individuals with ASD exhibit hyperserotonemia (i.e. increased levels of serotonin; Veenstra-VanderWeele et al., 2012). Whereas it has been found that 5-HTTLPR genotype is not related to the level of total blood serotonin in ASD (Betancur et al., 2002;Anderson et al., 2002) and that there is inconclusive evidence for the overall link between 5-HTTLPR genotype and susceptibility to ASD (e.g. Cook and Leventhal, 1996;Devlin et al., 2005;Zhong et al., 1999;Ramoz et al., 2006), we believe that heterogeneity in social function in ASD may be partially explained by a possible combination of decreased serotonin reuptake (not specific to ASD), increased prevalence of hyperserotonemia in ASD, and their effects on cortical organization. In line with this possibility, SERT knockout rats show reduced social play behavior (Homberg et al., 2007) and selective serotonin reuptake inhibitors (SSRIs) as well as SERT agonists (5-HT 1A ) affect sociability traits in BTBR T + tf/J (BTBR) mice, a mouse strain that exhibits behavior reminiscent of ASD characteristics (Gould et al., 2011). Future studies may benefit from monitoring the effect of 5-HTTLPR genotype and serotonin levels on the development of structural connectivity in individuals with ASD.
Along with the present results, the findings of Wiggins et al. (2014a), showing decreased amygdala habituation (i.e., more sustained activation over time) to sad faces but not happy, fearful, or neutral faces in individuals with ASD and low expressing genotype, signals the possibility of a compromised amygdala inhibition network. However, our study does not explain how individuals who are showing hyperconnectivity between the amygdala and the sACC in response to happy faces are able to habituate to happy faces at a similar rate to other groups with normative connectivity. One possibility is that, whereas studies point to the sACC as one of the major inhibitory structures, there are other structures and processes con-tributing to amygdala habituation that still need to be examined. Indeed, Etkin et al. (2011) found that dorsal areas of the ACC and the mPFC are involved in appraisal and expression of emotions and that both the sACC as well as ventral areas of the mPFC are involved in inhibition. Studies that simultaneously examine the appraisal and inhibitory effects of multiple brain structures on amygdala activity in response to diverse social situations may help explain the complexity of emotional response in ASD.
The present preliminary investigation has limitations. First, sample size was relatively small for testing the effects of genetics on behavior. Whereas the first studies linking 5-HTTLPR genotype to the amygdala found effects with sample sizes as modest as 23 (Fallgatter et al., 1999) and two independent groups of 14 participants each (Hariri et al., 2002), these results have replicated in a larger sample (N = 92; Hariri et al., 2005) as well as by independent groups (Bertolino et al., 2005;Canli et al., 2005;Dannlowski et al., 2007). It is possible that imaging the effect of endophenotypes such as neural activation or connectivity may be more sensitive in detecting the effects of genotype than directly relating genotype to self-reported behavioral measures, as stated by Canli and Lesch (2007). Meta-analyses such as Murphy et al. (2013) and Flint and Munafò (2006), however, highlight the need for replication studies and collaboration, as no single study has been able to gather enough data to be sufficiently powered to reliably demonstrate effects. As such, preliminary studies with modest effect sizes serve the purpose of offering routes of investigation for future replication within and between research groups. Second, the sphere chosen as the seed was selected based on the result of a previous 5-HTTLPR/ASD study conducted by our group (Wiggins et al., 2014a). This is a limitation because artifacts (e.g. scanner, motion) that could have affected Wiggins et al. (2014a) may have affected the present investigation as well. Amygdala dropout in data from certain participants made us unable to conduct PPI analyses using a larger, independent seed (structural left amygdala). Nevertheless, the present study corrected for possible motion artifacts by censoring volumes with more than 1 mm framewise displacement; a step that may help to avoid shared false positive results due to motion. Third, our sample differed in age, gender, and pubertal development among the four diagnosis-by-genotype subgroups. To control for this, we tested whether including age, gender, and puberty as a covariate affected our group analysis. Results continued to show that the ASD group with low expressing genotypes had significantly greater amygdala-sACC connectivity than ASD higher expressing genotypes and both TD genotype groups. Fourth, our sample's ethnic background consisted, largely, of Caucasian individuals (see Supplementary materials: Table 1). This may be a limitation given differences in processing other-race faces (Golby et al., 2001). However, the results of a Caucasian-only analysis yielded the same interaction pattern around a 6 mm sphere located in BA 25. Fifth, developmental transitions from childhood into adolescence have been shown to be important components in determining whether individuals with ASD display amygdala over-connectivity or under-connectivity in comparison to TD individuals (Nomi and Uddin, 2015). Analyses that consider the effects of genotype on connectivity across development will require a larger sample. Such an approach would provide a more complete understanding of neural changes occurring during this important developmental transition. Sixth, Hardy-Weinberg Equilibrium was not met in the TD sample. Whereas equilibrium was met in our ASD sample, our data were collected using convenience sampling and are not representative of a population with an expected distribution of 5-HTTLPR alleles. Participants included in this test were those who provided data for genotyping and successfully completed our fMRI scan. However, in analyses including all participants who provided data for genotyping (scanned and not-scanned), both groups meet Hardy-Weinberg Equilibrium. These data are preliminary and these limitations should be considered in replication attempts.
Future research may wish to investigate participant interpretation of the emotions presented. Our construal of the participants' reaction in response to happy faces (i.e. an aversion to happy faces) was not tested. Future studies may add to the present results by testing the relationship between distress to social contact and happy faces in ASD using various sources. This could be assessed through skin conductance response to test for physiological arousal and eye tracking to test for avoidance of gaze to the eyes. Moreover, polymorphisms do not operate alone in their effect on brain function. Researchers may benefit from examining the additive effect of genotype variants along the same metabolic pathway; a method used by Hernandez et al. (2016) in their investigation on the oxytocin receptor. This would give us a more informed picture of genetic influences on particular brain networks. Finally, a diffusion tensor imaging (DTI) study would inform our understanding of structural connectivity between the amygdala and the prefrontal cortex and its relationship to 5-HTTLPR genotype. Such research may highlight the relevance of the present finding to the differences in cortical connection size and number observed between ASD and TD individuals linked to serotonin (Casanova et al., 2002;Janušonis et al., 2004).

Conclusions
The present study provides preliminary evidence for an interaction between diagnosis (ASD vs. TD) and 5-HTTLPR genotype (low vs. higher expressing genotypes), where the ASD low expressing genotype group exhibits greater amygdala-sACC connectivity in response to happy faces than individuals with ASD and higher expressing genotypes and TD individuals of both low and higher expressing genotypes. The present findings are consistent with previous research showing that individuals with ASD and low expressing 5-HTTLPR genotypes display differences in brain function compared to higher expressing genotypes and TD individuals. Disparities in neural functioning have been found in terms of decreased amygdala habituation (Wiggins et al., 2014a) and increased default network connectivity with age  in a ASD low expressing genotype group. Moreover, the relationship between amygdala-sACC connectivity and aspects of social function in ASD may be better explained when considering 5-HTTLPR genotype. Sample size for this study is modest and thus present results should be considered preliminary until replicated with larger sample sizes and/or independent groups. This study is the first ASD-focused investigation that combines genetic polymorphism analyses and functional connectivity in the context of a social task. The current findings point to imaging as useful method for bridging the relationship between genetic contributions and social dysfunction in ASD.