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Effects of oxidative stress on erythrocyte deformability and fragility

Most methods established so far to estimate RBC deformability are hard to standardize and include high error of measurement. For our present investigation on the effects of oxygen (O2) and ozone (O3) on red blood cell (RBC) deformability we used the method of laser diffraction in a combination of image analysis. After exposing RBC to O2 and/or O3 mechanical fragility was investigated by filtration of the RBC suspensions (hematocrit 5%) through 3 micrometers pore filter under pressure of 200 mmHg. Fragility remained unchanged after oxygenation. Astonishingly O3 in concentration >= 7 (mu) g/ml improved mechanical fragility (decrease of hemolysis after mechanical stress).