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The effect of neonatal maturity on diversity of immunoglobulin heavy variable region gene

Aim:To study the effect of neonatal maturity on diversity of immunoglobulin heavy variable region (IgH) gene. Methods:DNA were extracted from cord blood of 10 very immature, 12 immature and 11 mature neonates, IgH gene was amplified using nested PCR technique, then cloned and sequenced. Results: V H1 is preferentially used in very immature and immature neonates, V H3 in mature neonates, and the usage of J H3 is highest in all neonates. There are an NDN length of (13.5±7.2), (17.8±7.5) and (23.3±8.2) bp, a somatic mutation (point mutation) rate of 15.8%, 23.6% and 29.5%, and an open reading frame of 78.3%、84.7% and 92.4% in very immature,immature and mature neonates, respectively. Conclusion:During the early life of neonates, V H-D-J H rearrangement of IgH gene is in an activated condition. Heterogeneity of IgH gene exists in neonates with different maturity, and humoral immunity is a step by step development with increasing maturity in neonates.[