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TRANSPORT OF SUGARS INTO MICROVESSELS ISOLATED FROM RAT BRAIN: A MODEL FOR THE BLOOD‐BRAIN BARRIER 1

Abstract— Microvessels (primarily capillaries) were isolated from the brains of rats 25‐35 days of age. This preparation was characterized by light, transmission, and scanning electron microscopy. Transmission electron microscopy revealed that the endothelial cell membranes were intact and were impermeable to horseradish peroxidase. However, scanning electron microscopy revealed that damage to the membrane occurred during isolation. The isolated microvessel preparations were metabolically competent as demonstrated by their ability to metabolize [14C]glucose.