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Survey of Protein Engineering Strategies

Protein engineering is altering the structure of a protein to improve or change its properties. This unit summarizes concepts for protein engineering using rational design, directed evolution, and combinations of them. Different strategies are presented for identifying the best mutagenesis method, how to identify desired variants by screening or selection, and examples for successful applications are given. This should enable researchers to choose the most promising tools to solve their protein engineering challenges. Curr. Protoc. Protein Sci. 66:26.7.1‐26.7.14. © 2011 by John Wiley & Sons, Inc.