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The Generation of Specific Cytotoxic Responses by Human Lymphocytes Requires Antigen and Activities Provided by a Lymphokine Supernatant‐

The requirements for the generation of specific cytotoxic responses by human lymphocytes have been investigated using as a model system the response to the ultraviolet light‐killed mouse tumour P815. These requirements are antigen (P815uv) and activities provided by a human lymphokine (LK) supernatant. Cells that give rise to non‐specific cytotoxicity can be separated on discontinuous Percoll density gradients after a 3‐day incubation of peripheral blood leucocytes with the lymphokine supernatant. Cells from which specific cytotoxic responses can subsequently be generated in the presence of P815uv and LK sediment at a higher density.