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Sensitivity of clonogenic cells of human ovarian ascitic cancer to antitumor drugs.

The sensitivity of human primary ovarian tumors to cyclophosphamid, 5-fluorouracil and adriamycin was investigated using tumor cell cultivation in agar diffusion chamber. It was found that the growth of clonogenic cells of human ovarian cancer was most strongly inhibited by cyclophosphamid and 5-fluorouracil. Tumor sensitivity to cyclophosphamid and cis-platin was significantly higher when tumor cells were cultivated in the presence of macrophages, the effect being dependent on the macrophage concentration. Tumor sensitivity to 5-fluorouracil was higher if prior to treatment macrophages were removed from the tumor cell suspension.