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[Isolation and characterization of an Enterococcus strain from Tibetan alpine meadow soil].

OBJECTIVE
We studied the physiological, biochemical properties and metabolism of Enterococcus strain CJ-1 from high-altitude soil in Namtso, Tibet.


METHODS
Strain CJ-1(T) was isolated from the soil of Namtso alpine meadow soil by Hungate anaerobic technique. Through physiological, biochemical and phylogenetic analysis, we identified the strain CJ-1.


RESULTS
Strain CJ-1 was Gram-positive and facultative anaerobe, 1 -1.5 microm in diameter. CJ-1(T) was atrichia nonmotile cocci, and always occurred in pairs. CJ-1(T) occurred in the presence of 0% -7% NaCl (optimum at 5%), pH 5.0 - 8.5 (optimum 7.0) and temperature between 10 degrees C and 50 degrees C (optimum at 25 degrees C). CJ-1 could metabolize many carbon sources including cellobiose, melezitose and ribose. Metabolites of cellobiose were lactic acid, acetic acid, propionic acid, butyrate, CO2, and little H2. The mol% G + C content of the genomic DNA was 39.2 mol%. 16S rRNA gene sequence analysis of the above strain showed the highest similarity of 95.9% with its closest phylogenetic neighbor Enterococcus aquimarinus. The strain can degrade cellobiose which act as intermediate metabolites in the methane fermentation process.


CONCLUSION
CJ-1(T) can degrade cellobiose. CJ-1(T) represents a novel species in the genus fermentation process. Enterococcus.