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The mechanisms of ethanol‐induced contraction of tracheal smooth muscle

We have shown that ethanol induces contraction of pulmonary artery smooth muscle. Therefore, we hypothesized that ethanol induces contractile responses of tracheal smooth muscle and extra cellular Ca2+ plays an important role on these responses. In vitro force measurements were performed in tracheal smooth muscle strips obtained from adult ferrets (n=33). TSM strips were treated with different concentration of ethanol (46 ‐ 322 mM). In another set of experiments strips were incubated in Ca2+ free Krebs ‐Hensleit solution or a Ca2+ channel blocker ‐ nifedipine was used and then treated with ethanol. The involvement of nitric oxide (NO) was studied by inhibition of nitric oxide synthase (NOS) using Nω‐nitro‐L‐arginine methyl ester. The ethanol induced concentration‐dependent contraction of TSM. Prior treatment of tissues with a single dose of ethanol increased the contractile responses of TSM to acetylcholine (p<0.05). Lack of Ca2+ in medium resulted with decrease of contractile force of the TSM likewise Ca2+ channel blocker. Inhibition of NOS significantly increased contractile responses (p<0.001).