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Isolation,Identification and Drug Susceptibility Test of Aermonas veronii from Cyrinus carpio

[Objective] The research aimed to provide theoretical basis for studying the infected bacterial diseases of Cyprinus carpio and their reasonable drug application in the clinic. [Method] The pathogen was isolated from the spleen of sick C. carpio from an aquafarm in Jilin Province and identified by conventional physiological and biochemical tests and 16 S r DNA gene sequencing. And the minimal inhibitory concentrations of 11 kinds of antibiotics on the isolated pathogen were determined by the micro-dilution method. [Result] A dominant strain was successfully isolated from the spleen of sick C. carpio,which was Gram negative. And the dominant strain was identified as Aermonas veronii by using sequence analysis. The challenged test results showed that LP1401 could cause C. carpio die. The results of drug susceptibility test showed that the dominant strain was resistant to sulfonamides,β-lactam and aminoglycosides,it was sensitive to chloramphenicols and enrofloxacin,and it was intermediary to doxycycline. [Conclusion] The research results could provide scientific basis for the prevention and control of C. carpio in A. veronii.