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The early discoveries of collagen–platelet interaction and studies on its role in hemostatic plug formation

be very complicated. It so happened that Helge Stormorken, who had been my classmate in the medical studies, contacted me, and asked if I was interested in starting a scientific project. He was a veterinarian before he started his medical studies, and had recently been appointed as professor in physiology at the Veterinary College of Norway. He was already an established scientist from the Owren group at Rikshospitalet where he later returned as head of the institute. He made great efforts in building up research activities in different fields at his Department of Physiology, and followed my work with great interest. I left my job in internal medicine at Rikshospitalet and began the studies on the circulation and the hemostatic process in rabbit mesenterium in 1960. Hugues [2,3] had in 1959 published studies on hemostatic plug formation in rabbit mesenterium. Stormorken and Professor Peter Hjort had become interested in these studies and after a visit to the Liege group, they wanted to pursue a similar approach. The Department of Physiology was then located in an old building and the experimental conditions were not as good as they are today, but we started up and created a similar setup to what they had in Liege. The exteriorized mesenterium in chloralose-urethan anesthetized rabbits were continuously irrigated with Zweifach’s solution kept at 38 � C, and the vessels were observed with a binocular microscope with a camera attachment. When cutting the vessels with a razor blade, the rapid adhesion of platelets to the vessel lips could be observed, followed by formation of the hemostatic plug which became impermeable within 3–4 min. The plug formation was studied under various conditions, but, unfortunately, these results were not published. Figure 1 shows a light microscopic picture of a hemostatic plug from these in vivo studies. As seen