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The Phosphorylation of Proteins in Hippocampal Slices: Effects of Noradrenaline and of Pretreatment with Kainic Acid

Abstract: Hippocampal slices were incubated in the presence of [32P]Pi, and protein phosphorylation was examined by means of sodium dodecyl sulfate‐gel electrophoresis. Incubation for at least 30 min with 300 μCi of [32P)Pi/brain slice gave rise to the phosphorylation of 8–10 protein bands. Most of these bands showed enhanced phosphorylation in response to noradrenaline. The basal phosphorylation of kainic acid‐pretreated hippocampal slices was enhanced two‐ to threefold compared with controls. There was also an additional increase in kainic acid‐pretreated slices in the response to noradrenaline. 8‐Br‐Cyclic AMP and phosphodiesterase inhibitors, such as papaverine or isobutylmethyl‐xanthine, had no effect on the phosphorylation patterns.