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[Study on HPLC fingerprint of 11 Taraxacum species in northeast of China].

OBJECTIVE
To study the RP-HPLC fingerprints of 11 plants in the genus Taraxacum for their quality control.


METHOD
The fingerprints were determined using an Agilent 1100 series instrument system. Chromatographic analyses were performed on a Kromasil 100-5 C18 (4.6 mm x 250 mm, 5 microm) analytical column,eluted with methanol and water containing 0.5% acetic acid as the mobile phases in gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 323 nm. The temperature of column was 35 degrees C. Eleven species of Taraxacum in northeast of China were detected respectively.


RESULT
Twenty-five common peaks existed in 11 RP-HPLC fingerprints. By comparing the retention time and the on-line UV spectra, peaks No. 10, No. 12, No. 16 and No. 25 were identified as chlorogenic acid, caffeic acid, p-coumaroy acid and luteolin respectively.


CONCLUSION
The analytical method with good precision and reproducibility can be useful in the quality control of Taraxacum plants.