{'fraction_non_alphanumeric': 0.02529960053262317, 'fraction_numerical': 0.0039946737683089215, 'mean_word_length': 5.539130434782609, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Detection of allele‐specific expression of N‐RAS oncogenes in human leukaemia cells

We report analysis of allele‐specific expression of N‐RAS transcripts in myeloid leukaemic cells and cell lines. Expression was assessed by an assay of reverse‐transcription/PCR combined with differential hybridization with mutation‐specific oligonucleotides. In cells from all patients with acute myeloid leukaemia (AML) and in myeloid cell lines HL‐60 and THP‐1, expression of both the wild‐type allele and the abnormal allele altered by a point mutation could be detected, albeit not always at comparable levels. This might be due for instance to allelic exclusion. The assay described provides a means of analysing the degree of expression of dominant oncogenes.