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Time dependency of ultrasound‐facilitated gene transfection

The use of ultrasound (US)‐facilitated gene therapy is increasing rapidly as a result of its high specificity and non‐invasiveness. However, the acoustic parameters that produce the most efficient transfection have not been established. The present study investigated the effects of time parameters [including pulsing strategy (on‐ and off‐times), exposure duration, pore opening time and expression duration] of US‐facilitated gene transfection.