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Effect of neem leaf extract on freshwater fishes and zooplankton community.

Neem (Azadirachta indica) is a medicinal plant of containing diverse chemical active substances of several biological properties. So, the aim of the current investigation was to assess the effects of water leaf extract of neem plant on the survival and healthy status of Nile tilapia (Oreochromis niloticus), African cat fish (Clarias gariepinus) and zooplankton community. The laboratory determinations of lethal concentrations (LC 100 and LC50) through a static bioassay test were performed. The 24 h LC100 of neem leaf extract was estimated as 4 and 11 g/l, for juvenile's O. niloticus and C. gariepinus, respectively, while, the 96-h LC50 was 1.8 and 4 g/l, respectively. On the other hand, the 24 h LC100 for cladocera and copepoda were 0.25 and 0.45 g/l, respectively, while, the 96-h LC50 was 0.1 and 0.2 g/l, respectively. At the highest test concentrations, adverse effects were obvious with significant reductions in several cladoceran and copepod species. Some alterations in glucose levels, total protein, albumin, globulin as well as AST and ALT in plasma of treated O. niloticus and C. gariepinus with 1 /2 and 1 /10 LC50 of neem leaf water extract compared with non-treated one after 2 and 7 days of exposure were recorded and discussed. It could be concluded that the application of neem leaf extract can be used to control unwanted organisms in ponds as environment friendly material instead of deleterious pesticides. Also, extensive investigations should be established for the suitable methods of application in aquatic animal production facilities to be fully explored in future.