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Lipid raft uptake at diaphragm muscle axon terminals

Lipid rafts are structurally unique components of plasma membranes, crucial for neural development and function. The uptake and transport of lipid raft associated molecules in motor neurons is poorly characterized under normal and pathologic conditions, including advanced aging effects. Lipid rafts are particularly important for motor neurons as they shuttle cargo between peripheral and central compartments. The cholera toxin subunit B (CTB) binds the cell‐surface ganglioside GM1, which is internalized and transported via lipid rafts in motor neurons, and thus is commonly used to investigate lipid rafts. We hypothesized firstly, that axon terminal cargo loading into lipid rafts will be different across fiber types and secondly, that uptake at axon terminals is not affected by activity at the neuromuscular junction (NMJ).