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Effect of Macronutrients, Cytokinins and Auxins, on in Vitro Organogenesis of Thymus vulgaris L.

The present study reports an efficient protocol for in 
vitro propagation of Thymus vulgaris L., an 
aromatic and medicinal plant in Morocco. Initially, we performed in vitro multiplication of Thymus vulgaris explants existing in the 
laboratory and obtained from micropropagation by shoot tip culture. Afterwards, 
we have evaluated the effect of six macronutrients. After that, seven 
cytokinins (Kin, BAP, 2iP, DPU, Adenine, Zeatine and TDZ) in three different 
concentrations (0.46, 0.93, 2.32 μM) have been evaluated to optimize cultures 
multiplication and elongation. Moreover, the effect of three auxins (IAA, IBA 
and NAA) at 0.57 μM, combined to 4 cytokinins (Kin, BAP, DPU and Ad.) at 0.46 
μM, on shoot rooting has been studied. Thereby, MS medium has been proved the 
most favorable for plantlets growing. Also, we found that the addition of 
certain cytokinins, specifically 0.46 Kin, 0.46 and 0.93 BAP, 0.46 2iP, 0.46 
DPU, 0.46 Ad. and 0.46 Zeat., ensures better multiplication and growth of 
vitroplants. In addition, multiplication and rooting of cultures were well 
optimized after addition 0.46 Kin + 0.57 IAA or NAA, 0.46 DPU + 0.57 IBA and 
0.46 Ad. + 0.57 IBA combinations to the culture medium. Lastly, plantlets with roots were successfully acclimatized to 
ex-vitro conditions and these latter served as a source to establish in vitro culture again.