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Low molecular weight urine proteins in cardiac pathology

To study the nature of proteinuria in the pathology of the cardiovascular system, we used disk electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate. The urine was subjected to electrophoresis without prior preservation. When the protein content in urine is less than 0.3 g / l, it was concentrated 16-30 times with polyethylene glycol. Densitometry of gel pillars was performed on an EPI-65M apparatus (GDR). The glomerular-tubular protein ratio (GTPK) was also calculated.