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Effects of intermittent fasting on health markers in those with type 2 diabetes: A pilot study

AIM To determine the short-term biochemical effects and clinical tolerability of intermittent fasting (IF) in adults with type 2 diabetes mellitus (T2DM). METHODS We describe a three-phase observational study (baseline 2 wk, intervention 2 wk, follow-up 2 wk) designed to determine the clinical, biochemical, and tolerability of IF in community-dwelling volunteer adults with T2DM. Biochemical, anthropometric, and physical activity measurements (using the Yale Physical Activity Survey) were taken at the end of each phase. Participants reported morning, afternoon and evening self-monitored blood glucose (SMBG) and fasting duration on a daily basis throughout all study stages, in addition to completing a remote food photography diary three times within each study phase. Fasting blood samples were collected on the final days of each study phase. RESULTS At baseline, the ten participants had a confirmed diagnosis of T2DM and were all taking metformin, and on average were obese [mean body mass index (BMI) 36.90 kg/m2]. We report here that a short-term period of IF in a small group of individuals with T2DM led to significant group decreases in weight (-1.395 kg, P = 0.009), BMI (-0.517, P = 0.013), and at-target morning glucose (SMBG). Although not a study requirement, all participants preferentially chose eating hours starting in the midafternoon. There was a significant increase (P < 0.001) in daily hours fasted in the IF phase (+5.22 h), although few attained the 18-20 h fasting goal (mean 16.82 ± 1.18). The increased fasting duration improved at-goal (< 7.0 mmol/L) morning SMBG to 34.1%, from a baseline of 13.8%. Ordinal Logistic Regression models revealed a positive relationship between the increase in hours fasted and fasting glucose reaching target values (χ2 likelihood ratio = 8.36, P = 0.004) but not for afternoon or evening SMBG (all P > 0.1). Postprandial SMBGs were also improved during the IF phase, with 60.5% readings below 9.05 mmol/L, compared to 52.6% at baseline, and with less glucose variation. Neither insulin resistance (HOMA-IR), nor inflammatory markers (C-reactive protein) normalized during the IF phase. IF led to an overall spontaneous decrease in caloric intake as measured by food photography (Remote Food Photography Method). The data demonstrated discernable trends during IF for lower energy, carbohydrate, and fat intake when compared to baseline. Physical activity, collected by a standardized measurement tool (Yale Physical Activity Survey), increased during the intervention phase and subsequently decreased in the follow-up phase. IF was well tolerated in the majority of individuals with 6/10 participants stating they would continue with the IF regimen after the completion of the study, in a full or modified capacity (i.e., every other day or reduced fasting hours). CONCLUSION The results from this pilot study indicate that short-term daily IF may be a safe, tolerable, dietary intervention in T2DM patients that may improve key outcomes including body weight, fasting glucose and postprandial variability. These findings should be viewed as exploratory, and a larger, longer study is necessary to corroborate these findings.


METHODS
We describe a three-phase observational study (baseline 2 wk, intervention 2 wk, follow-up 2 wk) designed to determine the clinical, biochemical, and tolerability of IF in community-dwelling volunteer adults with T2DM. Biochemical, anthropometric, and physical activity measurements (using the Yale Physical Activity Survey) were taken at the end of each phase. Participants reported morning, afternoon and evening self-monitored blood glucose (SMBG) and fasting duration on a daily basis throughout all study stages, in addition to completing a remote food photography diary three times within each study phase. Fasting blood samples were collected on the final days of each study phase.

RESULTS
At baseline, the ten participants had a confirmed diagnosis of T2DM and were all taking metformin, and on average were obese [mean body mass index (BMI) 36.90 kg/m Arnason TG et al . Impact of intermittent fasting on T2DM of IF in a small group of individuals with T2DM led to significant group decreases in weight (-1.395 kg, P = 0.009), BMI (-0.517, P = 0.013), and at-target morning glucose (SMBG). Although not a study requirement, all participants preferentially chose eating hours starting in the midafternoon. There was a significant increase (P < 0.001) in daily hours fasted in the IF phase (+5.22 h), although few attained the 18-20 h fasting goal (mean 16.82 ± 1.18). The increased fasting duration improved at-goal (< 7.0 mmol/L) morning SMBG to 34.1%, from a baseline of 13.8%. Ordinal Logistic Regression models revealed a positive relationship between the increase in hours fasted and fasting glucose reaching target values (χ 2 likelihood ratio = 8.36, P = 0.004) but not for afternoon or evening SMBG (all P > 0.1). Postprandial SMBGs were also improved during the IF phase, with 60.5% readings below 9.05 mmol/L, compared to 52.6% at baseline, and with less glucose variation. Neither insulin resistance (HOMA-IR), nor inflammatory markers (C-reactive protein) normalized during the IF phase. IF led to an overall spontaneous decrease in caloric intake as measured by food photography (Remote Food Photography Method). The data demonstrated discernable trends during IF for lower energy, carbohydrate, and fat intake when compared to baseline. Physical activity, collected by a standardized measurement tool (Yale Physical Activity Survey), increased during the intervention phase and subsequently decreased in the follow-up phase. IF was well tolerated in the majority of individuals with 6/10 participants stating they would continue with the IF regimen after the completion of the study, in a full or modified capacity (i.e. , every other day or reduced fasting hours).

INTRODUCTION
The incidence of type 2 diabetes mellitus (T2DM) is reaching epidemic levels worldwide, and correlates with rising obesity rates and sedentary lifestyles. In fact, it is predicted that 439 million adults will have diabetes by 2030 [1] . This is significant, as diabetes is closely associated with cardiovascular disease, retinopathy, neuropathy, and kidney disease. In turn, this places increasing stress on the health care system, and these patients utilize medical resources three to four times the amount of those without diabetes [2] . Modest weight loss and exercise regimes can both prevent the onset of T2DM and improve metabolic control [3] . According to most national diabetes associa tions and clinical practice, dietary interventions are considered essential in the treatment and prevention of diabetesrelated complications [4] . There are many types of dietary interventions people may use; one of which is intermittent fasting (IF). This is a dietary intervention that timerestricts feeding to 46 h and extends the overnight fast from 12 towards 18 or 20 h, may be a beneficial additional dietary strategy used in T2DM management.
After diagnosis, most individuals (depending on individual circumstances) with T2DM are given manage ment goals of an HbA1c below 7.0%, FPG 4.07.0 mmol/L, and postprandial levels of 5.010.0 mmol/ L [3,5,6] . Selfmonitoring of blood glucose (SMBG) using glucometers can be critical for patient feedback and recognition of glucose control, as symptoms poorly predict glucose levels alone. Glucose goals are typically reached through an individualized treatment regime including lifestyle (diet and exercise) and medication use, yet a great number fail to reach, or maintain, these diabetic goals. As a result, a plethora of medication combinations are tried, as are weight loss approaches including consideration for bariatric surgery for morbidly obese individuals. Given the intense focus on weight and dietary measures, IF has the potential to be included in the armament in the fight to improve SMBG levels while contributing to weight loss in those in whom it would be beneficial.
In addition to glucose levels and body weight, there are other important aspects of T2DM worth considering. First is the degree of insulin resistance, which theore tically contributes to the difficulty of maintaining euglycemia [7] . Outside of the research clinic, insulin resistance can be measured in healthy patients using surrogate index measures derived from glucose and insulin measures in the fasting state. These surrogate index measures include glucose/insulin ratio, log fasting insulin, Homeostasis Model Assessment (HOMAIR), log HOMAIR, and Quantitative Insulin Sensitivity Check Index (QUICK1). A decrease in insulin resistance can improve glucose control, and exercise and weight loss both favorably decrease resistance states. Hence, we measured HOMAIR, which has the most agreement with the clamp technique in assessing insulin resistance in T2DM patients [8] , to help determine if IF may be beneficial.
Although the causal role is unknown, an association between chronic low-grade inflammation and diabetes has been shown to occur [9] . Creactive protein (CRP) is a biomarker used to determine if inflammation is present. Elevated levels of CRP have been associated with insulin resistance, nephropathy progression and elevated fasting glucose in diabetes patients [10,11] . It is also known that CRP can be decreased with dietary therapy [12] . Hence, we measured CRP to see whether IF favorably decreased this inflammatory marker.
It can be challenging to measure dietary and ex ercise habits in clinical studies, and there is no defined intervention that is shown to be better than one an other [13] . Monitoring devices worn daily are acceptable methods of tracking physical activity, with accelerometers being the current gold standard [14] . An alternative method to use in freeliving adults is a questionnaire. Although not the gold standard, they have been shown to be a reliable method. The Yale Physical Activity Survey (YPAS) is a particular questionnaire that has shown to be reliable for capturing physical activity [14,15] . The YPAS has considerable testretest reliability which makes it a useful tool for a repeated measures design in free livingadults [16,17] . Hence, the YPAS questionnaire was employed to track physical activity in this study.
The gold standard for measuring energy intake is the DoublyLabelled Water method (DLW) [18] . However, this is difficult to use in those who are not experienced. One alternative to the DLW is the remote food photography method (RFPM). The RFPM has been shown to be an efficient and accurate method of capturing dietary intake in freeliving adults [19,20] . Hence, the RFPM for 3 d in each of the study phases was utilized in this study to capture estimates of energy intake.
The Canadian Diabetes Association (CDA) publishes Clinical Practice Guidelines that currently recommends that individuals with T2DM follow the Canada Food Guide for nutritional needs [21] . Further, the CDA pro motes various other nutritional strategies, such as portion control, carbohydrate counting, and grouping foods according to their glycemic index [4] . While these are all appropriate recommendations, some people have difficulty grasping these suggestions and fitting them into their diet [22] . Even an activity as simple as calorie counting has been reported to lead to an increase in selfperceived psychological stress and cortisol levels, effects not seen when participants restricted calories unintentionally [23] . Another valuable resource for helping attain nutri tional and diabetic goals is for patients to see a dietitian and/or attend a selfmanagement program. However, many barriers exist for patients to access these programs or adhering to dietary advice [24] . Some of the barriers listed by people with diabetes range from feelings of helplessness and frustration, to not attaining desired glycemic control, to not being able to accommodate sug gestions regarding food restrictions [22,25] .
To overcome some of the barriers that some dia betes patients face with dietary interventions, alter native solutions should be explored. An example of an alternative dietary solution is IF. There are many variations of IF, but it is essentially restricting caloric intake to a specified period of time. One method of IF is to have people restrict their caloric intake for 18 to 20 h per day and eat ad libidum during those other 4 to 6 h. The feeding period will usually occur midday to early evening, and an increased protein intake may or may not be recommended to help increase satiety. During the fasting period, people are allowed to consume water, coffee, or tea. With this method of IF, caloric intake occurs when there is a diurnal peak in insulin sensitivity, and, similarly, a diurnal peal in cortisol levels during the fasting period. This may theoretically benefit glucose control. Similar protocols have been shown to have beneficial effects in non-diabetic populations with varying effects on glucose uptake, lipid levels, cortisol levels, and body fat [2629] . Previous studies have shown that an IF intervention whereby all of one's daily calorie consumption are consumed in a fourhour window led to participants feeling too full and some weight loss, despite recommendations to consume more calories [26,27] . Hence, it is possible that IF can lead to a spontaneous caloric deficit in adult patients in their homes [30] . However, another study found that an IF intervention led to worsening of glycemic control [27] , hence there are conflicting results as to its overall effects in a healthy individuals.
IF has received increasing attention for its potential therapeutic role in the treatment and prevention of cardiovascular disease and T2DM. However, we could only identify 2 studies in the literature evaluating the effects of IF in T2DM, although other trials are ongoing. The first evaluated a Mediterranean-style diet, with and without breakfast, on postprandial glucose, insulin, triglycerides and gastric inhibitory polypeptide in individuals with T2DM over a single day [31] . The Mediterranean lunch without breakfast revealed no increase in glucose, insulin, or triglycerides during the breakfast period (despite coffee intake), which did significantly increase above baseline during the comparator arms of lowfat and lowcarbohydrate breakfasts. Another 12wk had people undergo an IF fast whereby they only consumed caloric content in the morning and early afternoon, and in this study, a decrease in FBG, HbA1c, and an improved response to an OGTT were observed [32] . Given these potential benefits, we designed a shortterm observational pilot study to assess tolerability, safety, and specific anthropomorphic, biochemical and blood glucose health benefits of daily IF in patients with T2DM, and measured their persistence upon return to an unstructured diet.

Recruitment
Participants were recruited from within the Saskatoon Health Region, which serves a population catchment of approximately 325000 people. Posters were delivered to general practitioners' offices in Saskatoon, as well as the 3 hospitals within the city of Saskatoon. Advertisements to the general public were placed in local newspapers and via internet outlets (i.e., Kijiji) alerting them of the study.
Individuals with a diagnosis of T2DM (confirmed by fasting glucose > 7.0 mmol, HbA1c > 6.5%, or OGTT > 11.0 mmol) and between the ages of 1865 were eligible to enroll in this study. Certain medical conditions were excluded from enrollment, such as the presence of ischemic heart disease or heart failure, chronic inflammatory diseases, chronic infections, moderate to severe renal disease (GFR < 45), uncontrolled hyper tension and hypoglycemic unawareness. Lastly, parti cipants were excluded if they currently managed their diabetes with either insulin or glyburide due to their increased risk of hypoglycemia.

Study design
Interested participants that met inclusion criteria pro vided written consent and were educated on study procedures, and the risk, detection and management of hypoglycemia. The study was divided into 3 phases, baseline (runin), intervention, and followup ( Figure  1). During the baseline period, participants engaged in their normal dietary patterns (breakfast, lunch and dinner) for a period of 2 wk. During the intervention phase (weeks 34), participants were instructed to follow the IF meal timing pattern. This consisted of a fasting goal for a period of 1820 h per day, with ad libidum zerocalorie coffee, tea, and water intake during fasting hours being permitted. During feeding time, participants were allowed to eat whatever they chose, but were encouraged to include at least 1/3 plate of protein to promote satiety (visual representations provided during training). The intervention phase was followed by a 2 wk followup phase with normal dietary patterns. There were no embedded criteria for weight loss, calorie restriction, or changes to exercise habits for the participants.

Assessment and evaluation
Self-reporting: Hours fasted, SMBG, caloric intake and exercise were all selfreported. Throughout all study phases, participants reported SMBG 3 times daily (fasting morning, random afternoon, and postprandial evening) with the use of a glucometer and logbook that was provided to them free of charge. In the same logbook, they also kept a diary of total consecutive hours fasted each day. In each of the three time periods (baseline, intervention, followup) participants completed a random 3d food diary using the RFPM. During these days, participants received customized text message prompts by study staff to ensure compliance with RFPM. Participants responded to all text prompts to confirm that they had adhered to RFPM, as well as sent images of their food (before and after consumption to capture food waste). Physical activity/spontaneous energy expenditures were captured using the YPAS tool at the end of each of the study phases.

Biochemical and anthropometric measurements:
Participants underwent fasted blood draws on the last day of the baseline phase, intervention phase, and followup phase. Fasting insulin, fasting plasma glucose (FPG) (with subsequent calculation for HOMA IR) and CRP were measured. On each of these days, participants also underwent anthropometric mea surements (height, weight, blood pressure, waist circum ference). These measurements were all performed by the same individual.

Statistical analysis
All statistical procedures were performed on SPSS v. 22 and STATA v. 13. Data preparation was done using Excel 2011 and STATA v. 13. Significance was set at alpha = 0.05 (95%CI) for all tests. Repeat measures ANOVA were performed for measuring changes in anthropometric and biochemical changes.
The group means and standard deviations of days 1 through 42 (6 wk total study time) were calculated individually for three daily measurements: fasted morning (M), random afternoon (A), and postprandial evening (E) SMBG measurements. Linear and quadratic regression of group means and standard deviations were used to explore the relationship between study phase and SMBG.
OLR was used to explore the impact of relative daily fasting duration on SMBG. Cutoffs for OLR were created using standards for the diabetic fasting goal (< 7.0 mmol/L) and frank hyperglycemia (> 11.1 mmol/L), with an additional midpoint (9.05 mmol/L). The variable created for OLR was the hours fasted difference (HFD), the difference between actual hours fasted and the average hours fasted during the baseline phase (Table 1).
No category was created to represent hypoglycemic events, as none were recorded throughout the duration of the study.

Baseline participant characteristics
Summarized in Table 2 are the anthropomorphic and biochemical measurements (with standard deviation) of the ten participants at study entry. Normal values for clinical and biochemical parameters are referenced. Subjects had a mean age of 53.8 years old (ranging 4462) and BMI of 36.9 (range of 2845 kg/m 2 ), the latter corresponding on average to Class II obesity, with very high disease risk compared to normal weight individuals. Insulin resistance was confirmed, with a mean HOMAIR of 6.91, and baseline fasting blood glucose levels were above the goal of < 7.0 mmol/L (mean 7.45 mmol/L). A nonspecific biochemical marker of inflammation (CRP) was also elevated at baseline in this cohort. All participants took daily metformin as part of their diabetic management, eight as monotherapy. One participant was also taking gliclazide while another was also using liraglutide.
The clinical and biochemical changes resulting from a 2 wk dietary intervention of IF are shown in Table 3. The means of the measured biochemical and anthropomorphic outcomes of all subjects were cal culated for each variable within the three phases of the study, with standard deviations shown: Phase 1 (baseline), Phase 2 (intervention) and Phase 3 (follow up). Repeat measures ANOVA comparisons between study phases were done to reveal significant differences (P < 0.05). Clinical measures revealed that IF decreased mean body weight, BMI, blood pressure, and waist circumference as compared to baseline with significant changes only for body weight (1.4 kg; P = 0.009) and BMI (0.52; P = 0.01). The beneficial changes observed   Table 1 Cut-off points created to explore the impact of fasting on self-monitored blood glucose SMBG: Self-monitored blood glucose.  were not sustained once the IF was complete. After a return to normal diet (Phase 3, followup), there was an inflection back toward baseline values for all parameters except a further nonsignificant decrease in waist circumference (0.3, P = 1.0). All participants increased their fasting time during the intervention phase. The daily hours fasting increased from a baseline of 11.6 to 16.8 h during the intervention phase (+5.2 h; P < 0.001), and essentially returned to baseline (11.5 h) after the followup period. The averages of SMBG reported daily from home glucometers decreased during the intervention phase for fasting morning, afternoon and postprandial time points (Table 4). Pooled averages of SMBG taken three times daily throughout the study are presented by study phase, indicating the % change from baseline to IF (Phase 1 to 2), and IF to followup (Phase 2 to 3).

Measurement Mean ± SD
IF improves morning fasted glucose levels and de creases postprandial variability. To further investigate potential benefits of IF on glucose levels, daily (rather than grouped by study phase) SMBGs were plotted over the 42 d study (Figure 2A). To investigate the glucose variances from the mean for fasting morning glucose levels, the KolmogorovSmirnov (KS) test was used. Figure 2B indicates that the daily distribution of morning SMBG was greatest during the intervention as compared to baseline (P = 0.002), or followup (P = 0.003) phases. Lastly, a similar assessment of the daily variation from the mean for evening postprandial values ( Figure 2C) demonstrates wide scatter throughout all phases, with decreases in glucose variability from baseline to intervention, as well as from intervention to followup phase. A significant difference was found for evening SMBG distributions (P = 0.044) between the intervention and followup phases only (all other phases P > 0.1). There were no statistically significant differences observed between any phases for the afternoon SMBG distributions (P > 0.1).
IF enhances the proportion of fasting SMBG at goal and decreases postprandial glucose excursions. Raw SMBG counts and percentages of SMBG residing within defined glucose categories were tabulated for each study phase. Table 5 reflects morning fasted and evening postprandial SMBG. SMBG results were placed within four discrete glucose ranges including normal/ target fasting (< 7 mmol/L), frank hyperglycemia (> 11.1 mmol/L), and an equal cutoff bridging the two (cut off at 9.05 mmol/L) to determine the cause of the increased variation noted for morning SMBG during IF. The distributed results for fasting SMBG readings Table  5 highlight that the incidence of atgoal SMBG < 7.0 mmol/L increased 2.5fold during the IF intervention (13.8% baseline increased to 34.1% during IF). Also noted during IF was the overall decrease in fasting hyperglycemia (> 7.0 mmol/L) that was offset by a greater incidence of elevated fasting levels (> 11.1 mmol/L noted at 0.8% baseline, increasing to 7.1% during IF). The improvement of readings at target glucose levels was lost during follow up, upon return to normal eating patterns.
Considering the pooled incidence of evening post prandial SMBG up to and including 9.05 mmol/L as "atgoal" for postprandial blood glucose, IF resulted in a higher proportion in the desirable range (    at baseline. Similar levels (54.1%) were found at goal during the followup phase, as at baseline. The proportion of SMBG > 11.1 mmol/L changed little with IF. The greatest change was found in the decrease in SMBG between 9.0511.1 mmol/L, specific to the IF phase.
The increase from baseline, rather than the absolute number, of hours fasted improves the probability of optimal glucose control. HFD was calculated for baseline and intervention phases to explore the relationship between the increase in hours fasted from baseline and SMBG improvements. In order to determine the relationship between glucose levels and time spent fasting, OLR was performed on the nonequalized data sets from the 8 participants who had completed both their dailyhoursfasted and full SMBG logs. As presented in Figure 3, these results support the previous SMBG regression findings; mean morning SMBG readings decreased, and improvements in these readings were primarily a result of increased fasting hours from baseline. The HFD and OLR models showed a statistically significant association between change in HFD with decreased SMBG morning readings (χ 2 likelihood ratio = 8.36, P = 0.004) but not for afternoon or evening SMBG readings (P > 0.1, Table 6). The IF phase was associated with spontaneous decreases in caloric intake and increases in physical activity. To calculate energy intake during the study, five (baseline and intervention) and four (followup) participants recorded their food intake via the RFPM. This was performed for 3 d of each study phase. From this, estimates of the total kcal/day, and the pro portionate contribution from protein, carbohydrate and fat was determined as shown in Table 7. Total kcal/day decreased 18.6% with the IF intervention, and further decreased 6% into the followup phase. Carbohydrate and fat intake decreased 33% and 36% respectively during the IF period, whereas protein intake remained constant. To estimate physical activity during the study, all participants completed the YPAS once during each of the three phases. From this survey, an estimate of physical activity (kcal/wk) during the three study phases was determined. During the intervention phase, physical activity increased (+1856.3 kcal/wk), but then decreased at study end (in the followup phase; 2449.6 kcal/wk).  Figure 2A, with the three phases indicated. Means were calculated from pooled SMBG data from the nine individuals that provided complete log sets. Figure 2B represents the daily variance from the mean for fasting morning SMBG, days 1-42, using the Kolmogorov-Smirnov (KS) test. Figure 2C shows the daily variance from the mean for evening postprandial SMBG values on days 1-42. Inflection points of quadratic equations were calculated using the formula [f 1 (y) of C + Ax + B × 2 = 0] rounded down to the nearest full integer. A: Mean morning fasted SMBG; B: Morning fasted SMBG variability; C: Evening random SMBG variability. SMBG: Self-monitored blood glucose.   Table 6 Ordinal Logistic Regression: Relationship between Hours Fasted Difference and morning, afternoon, and evening self-monitored blood glucose SMBG: Self-monitored blood glucose. SMBG: Self-monitored blood glucose.

Key findings
Many individuals with T2DM would benefit from a simple and accessible nutrition intervention that is simple to implement and teach, and improves their glycemic con trol. Teaching diabetes patients about IF only required between 1530 min of the study coordinator's time, hence it was easy to teach. Although short term (2 wk IF intervention), and without oversight (selfreported and selfcontrolled eating hours), the intervention resulted in significant improvements in diabetic glucose control. The IF phase yielded a significant increase in the incidence of fasting blood sugars at target (34.1% vs 13.4% baseline), and favorable decreases in postprandial hyperglycemia (39.4% vs 47.4% baseline). There was also a spontaneous 18% decrease in caloric intake and increase in energy expenditure (+1856 kcal/ wk), coinciding with a significant decrease in weight (P < 0.009) and BMI (P = 0.01). A strong association between the increase in hours fasted from baseline, and the probability of attaining a normal fasting glucose level was found (+LR 8.36), despite few individuals reaching the 1820 h fasting goal. We did not find statistically significant changes to blood pressure, insulin resistance or inflammatory markers after 2 wk of IF, although all trended towards normal during this phase. Importantly, the diet was found to be tolerable and safe, with zero incidences of hypoglycemia. Upon a return to normal eating habits, the improve ments to fasting glucose levels reversed to baseline, a trend also seen for the nonsignificant improvements in CRP, HOMAIR and BP. The sample size of this study is too small to determine if the sustained decreases in waist circumference, postprandial glucose variability, and energy intake into the followup period are meaningful.

Comparison to other dietary outcomes
The most similar study to ours that we could identify in the literature evaluated IF was a 3 mo crossover study in T2DM patients [32] . These studies differed with respect to when participants were instructed to undergo the prolonged fasting period (participants in our study were allowed to selfselect their fasting period, and chose late afternoon to early evening). Similar trends were observed with respect to weight loss and decreased waist circumference, whereas the study by Kahleova et al [32] also showed significant improvements in fasting insulin (and presumably insulin resistance), which our did not. In contrast, other studies in nondiabetic, normalweight participants, have shown that IF resulted in increased insulin resistance, fasting glucose, and lipids, despite weight loss occurring [26,27] . These different observed effects between diabetic and nondiabetic individuals to similar dietary changes may be related to the differences in body weight and hyperglycemia Figure 3 Positive relationship between hours fasted and morning glucose levels. Ordinal logistic regression (OLR) tested the association between the increase in hours fasted from baseline, and the morning fasting glucose levels within the four glucose ranges noted. OLR was performed using data from the 8 individuals who had completed both their full SMBG logs and daily hours fasted logs. OLR models used the non-equalized data. Hours Fasted Difference (HFD) was calculated for baseline and intervention phases only. SMBG: Self-monitored blood glucose.

Measure
Baseline (mean ± SD) Intervention (mean ± SD) Follow-up (mean ± SD)   between the studies, or due to as yet unknown factors. This work demonstrates that there is a correlation between hours fasted and improvements in fasted glucose levels, leading us to question if simply skipping breakfast (and thereby prolonging the fast) would improve T2DM control. A study by Thomas et al [33] tested the effects of skipping breakfast in obese women (without diabetes). They found that when regular breakfast eaters skipped breakfast, they had greater insulin and free fatty acid excursions in response to lunch as opposed to days when both breakfast and lunch were eaten [33] . In contrast, those who regularly skipped breakfast did not experience irregular responses at lunch, regardless of whether breakfast was eaten or not [33] . Clearly, extended fasting will not benefit all and has the potential to worsen measures of health if applied to patients with T2DM.
We also query if the observed benefits in this study arise from altering the normal eating rhythm, rather than inducing a net negative energy state (such as during fasting, or exercise). Although some evidence from animal studies suggest that biological clocks may be altered as a result of changes in feeding habits [34,35] , evidence of the effects of feeding entrainment on glycemic excursions in humans are lacking.

Strengths and weaknesses of study
Our study involved ten individuals (9 female, one male) who present with the typical characteristics of the majority of the worlds' T2DM population; middle age, overweight, with insulin resistance and average fasting blood sugars above goal. There are several unique aspects to this study that provide useful information, not the least of which was the patientcontrolled meal timing and content, all without caloric restriction. It is the first study designed with patients directly transitioning from their normal dietary habits, to an IF intervention, to a followup period to see if any of the effects were sustained. Also, the capturing of SMBG readings along with the actual total hours fasted allowed us to make inferences on this relationship, which has not been done before. Another strength is our use of the RFPM, which has been shown to have good validity [19,20] . Adherence with the study protocol, as reflected by SMBG and the recorded hours fasted, was high. Also, as a surrogate for tolerability of the IF intervention, 7/10 participants viewed the intervention as tolerable when asked, and 6/10 stating they would consider continuing a modified form of IF.
The greatest weakness was the lack of power from low recruitment, which was the limiting factor in determining clear effects of IF on markers of health in T2DM, or the detection of sustained effects during followup. An uneven distribution between females (n = 9) and males (n = 1) is also a limiting factor for generalizability. Also of importance to note was the failure to reach the goal of 1820 h of fasting per day, as these longer fasting times may have accentuated health benefits more clearly. Ultimately, the 16.8 ± 1.2 h fasting during Phase 2 of the study is perhaps a true reflection of the feasibility of fasting times for this diet in freeliving adults. Further a longer duration of the study phases would have allowed for comparative HbA1c measures to be done, a widely accepted marker of average changes to overall glucose control reflecting the previous three months. Lastly, it is possible that all of the SMBG testing that patients did may have in itself led to behavior changes that affected the overall recorded glucose levels. Any information learned from this observational pilot study can be used to inform a larger, longer, observational or interventional trial.

Future directions and developments
Our understanding of the association between feeding entrainment and diurnal rhythms is limited, and requires further study. Also, our study took blood draws from patients at specific time points, for study ease. Ideally, patients would have blood draws performed regular over a 24 h period so that fluctuations could be noted that may be due to diurnal variations, as the consump tion of calories changes over the IF period. Since our participants only had blood draws performed in the morning, some biochemical changes that may have occurred during other time periods would have been missed. It would also be ideal for future studies to have a longer study period which would allow for significant weight loss to occur, and then see if any biochemical changes are sustained during an extended followup period. Of course, a larger sample size would allow for a more robust conclusion as to the association between SMBG and IF and would shed som light on the true effects on morning, afternoon and evening SMBG results.

ACKNOWLEDGMENTS
The authors would like to acknowledge the help and support that Dr. Hasitha Welihinda provided for this study. Further, we would like to thank the Department of Medicine as well as the College of Pharmacy and Nutrition, University of Saskatchewan, for support of this project. The authors would also like to acknowledge Sanofi Canada for the provision of glucometers and test strips.

Background
Intermittent fasting (IF) is an increasingly popularized dietary method amongst varying population groups. The reasons for its increasing popularity are multifactorial, but one of which is for weight loss. Although research has been performed evaluating an IF intervention in animals and healthy subjects, there is very little known about its effects, beneficial or otherwise, in those with type 2 diabetes.

Research frontiers
As the prevalence of diabetes continues to increase, new dietary measures are necessary to pursue, as one size does not fit all. It is possible that IF may be a good option for people with diabetes alongside other popularized diets such as the Mediterranean diet, the DASH diet, and the Newcastle diet. With type COMMENTS