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[Study on the influence factors of exhaled breath condensate pH detection of asthma children].

OBJECTIVE
To explore influence factors of exhaled breath condensate (EBC) pH detection and establish standard method for EBC pH detection of asthma children.


METHODS
EBC samples were collected using Rtube EBC collection system, and pH values were detected by microelectrode method.


RESULTS
The temperatures of sampling condenser tube (from - 18 to 4 degrees C) and duration of collection (from 10 to 30 minutes) has no significant effect on EBC pH of asthma children. After degasification with argon for 4 minutes, the EBC pH became stable. The day to day precisions (relative standard deviation) were 0.74% -1.01%, and the relative deviations between theoretical values and detection values were 0.11% - 0.75% in determining different level of pH standard solutions. The EBC samples for pH detection could be store at - 20 degrees C for 6 months.


CONCLUSION
The established method by standardizing influence factors is accurate and convenient. It can be used in the detection of EBC pH of asthma children.