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Yarn Reinforced Gelatin Hydrogel As A New Scaffold For Tissue Engineering

We fabricated yarn reinforced hydrogel using electrospinning technique. The yarns were characterized by scanning electron microscopy (SEM). We then performed cell studies including measurements of cell viability and proliferation on the yarn reinforced hydrogel, using human adipose derived stromal/stem cells (hADSCs). hADSCs responses on the yarn reinforced hydrogel exhibited suitable cell viability and proliferation for tissue engineering applications. We also investigated the effect of yarns on the cell orientation using F-Actin staining. The results of F-Actin staining showed that the cells oriented along the yarn direction. This study introduces the yarn reinforced hydrogel as a novel scaffold with the potential to use in fibrous tissue engineering applications, such as regeneration of the annulus of the intervertebral disc.