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Diffusion-weighted MRI in solitary pulmonary lesions: associations between apparent diffusion coefficient and multiple histopathological parameters

Apparent diffusion coefficient (ADC) from diffusion-weighted imaging (DWI) has gained wide attention as potential tool for differentiating between malignant and benign solitary pulmonary lesions (SPLs). The overall effects of multiple histopathological parameters on ADC have not been elucidated, which may help to explain the overlapping of ADC between malignant and benign SPLs. The study sought to explore associations between ADC and histopathological parameters in SPLs, and to compare diagnostic capability of ADC among different types of SPLs. Multiple histopathological parameters (cell density, nuclear-to-cytoplasm ratio, necrotic fraction, presence of mucus and grade of differentiation) were quantified in 52 malignant and 13 benign SPLs with surgical pathology available. Cell density (β = −0.40) and presence of mucus (β = 0.77) were independently correlated with ADC in malignant SPLs. The accurate diagnosis rate of squamous carcinomas, adenocarcinomas without mucus and malignant tumors with mucus was 100%, 82% and 0%, respectively. Our study suggested that cell density and presence of mucus are independently correlated with ADC in malignant SPLs. Squamous carcinoma maybe more accurately diagnosed as malignancy by ADC value. Malignant SPLs with mucus and adenocarcinomas with low cell density should be kept in mind in differentiating SPLs using ADC because of insufficient diagnostic capability.

diffusion and allows for microstructural characterization of tissue through probing changes of barriers to diffusion, which can be quantified as apparent diffusion coefficient (ADC) 17 . Although malignant pulmonary lesions have significantly lower ADC value than benign lesions in summary statistics 11 , ADC value may be not sufficiently robust to differentiate SPLs prospectively, because of diverse diagnostic thresholds reported in prior studies, confounding overlap with broad ranges, high heterogeneity in pooled sensitivity and specificity 12 .
Imaging-histopathological comparison study is helpful to identify the cause of overlapping ADC values between malignant and benign SPLs. Several histopathological parameters, such as cell density, nuclear-to-cytoplasm ratio (NCR), necrotic fraction and grade of differentiation, have been previously quantified and some were shown to be significantly correlated with ADC value in malignant SPLs [18][19][20][21][22] . However, to the best of our knowledge, the overall effects of these histopathological parameters on ADC value have not been elucidated. In this study, we aim to: (1) explore associations between ADC value and multiple histopathological parameters in SPLs, and (2) compare the diagnostic capability of ADC value and independently histopathological parameters among different types of SPLs.

Results
Included SPLs and pathological findings. From June 2014 to October 2016, 108 participants were consecutively recruited and underwent chest MRI. Two participants without histopathological diagnosis and 13 participants with inadequate image quality (4 with severe magnetic susceptibility artifacts and 9 with severe motion artifacts) were excluded. Of the remaining 93 SPLs (from 93 participants), 71 (76%) were malignant and 22 (24%) were benign. Characteristics of included participants and SPLs are shown in Tables 1 and 2. No significant difference was found in median size between malignant and benign SPLs (28 mm vs. 26 mm, P = 0.28). The major pathological diagnosis of malignant SPLs are adenocarcinoma, adenosquamous carcinoma, squamous cell carcinoma, small cell carcinoma, and metastasis. The types of benign SPLs include chronic inflammation, granuloma, organized pneumonia, chondroid hamartoma, bronchopulmonary sequestration, sclerosing hemangioma, hyperplastic lymphoid tissue, native benign smooth muscle lesion, and undetermined.
Associations between ADC value and multiple histopathological parameters in malignant and benign SPLs. The associations between ADC value and histopathological parameters were analyzed in 52 malignant SPLs and 13 benign SPLs that were surgically excised. Cell density (β = −0.40, P < 0.001) and presence of mucus (β = 0.77, P < 0.001) were independently correlated with ADC value in malignant SPLs, whilst no significant associations were found in benign SPLs (see Table 3). . Correlation analysis between ADC value and single histopathological parameter, and type-specific accurate diagnosis rate were analyzed in 9 squamous carcinomas, 27 adenocarcinomas without mucus and 5 tumors with mucus (3 adenocarcinomas with mucus production, 1 mucinous adenocarcinoma and 1 mucoepidermoid carcinoma), respectively (see Tables 4 and 5). There was significant correlation between ADC value and cell density in both squamous cell carcinoma (r = −0.85, P = 0.004) and adenocarcinoma without mucus production (r = −0.57, P = 0.002). The accurate diagnosis rate of squamous carcinomas was 100% (9/9), which was non-significantly higher than that [82% (22/27)] of adenocarcinomas without mucus (P = 0.21). Significant difference was found in mean ADC value (0.98 × 10 −3 mm 2 /s vs. 1.08 × 10 −3 mm 2 /s, P = 0.04) and mean cell density (373 vs. 287, P = 0.003) between them. All five malignant tumors with mucus could not be accurately diagnosed as malignancy according to optimal cut-off value. There was no significant difference in mean cell density between malignant tumors with mucus and adenocarcinoma without mucus (275 vs. 287, P = 0.69) or between malignant tumors with mucus and squamous   Table 6. There was significant difference in mean ADC value between inflammatory and noninflammatory benign SPLs (P = 0.001). The accurate diagnosis rate for noninflammatory benign SPLs was 100% (6/6). Because of the small sample size, we did not explore the association between ADC value and single histopathological parameter in benign subgroups.

Discussion
Associations between ADC value and multiple histopathological parameters in SPLs was analyzed in this imaging-histopathological comparison study. We found that cell density and presence of mucus were independently correlated with ADC value in malignant SPLs, but NCR, necrotic fraction and grade of differentiation were not. There was no significant association between ADC value and histopathological parameters in benign  (3,9)     SPLs. ADC values from malignant SPLs with mucus or adenocarcinomas with low cell density were overlapping with ADC values from benign SPLs, which could induce false negative diagnosis. Water diffusion in biological tissue is complicated and highly dependent on the ratio of extracellular to intracellular space, which can be reflected by cell density and NCR. Increasing cell density will decrease the extracellular space, and result in restriction of water diffusion in extracellular space. Increasing NCR may increase water diffusion in intracellular space, since intranuclear water has a higher diffusion coefficient than cytoplasmic water 23 . However, tumors with high NCR also tend to have a small amount of extracellular space. Chen et al. 21 reported a negative correlation between NCR and ADC value in lung cancer. Our result indicated that confounding effect existed and water diffusion in intracellular space was likely less important in determining ADC value. In contrast, ADC value was independently affected by fraction of extracellular space as quantified by cell density. Apart from malignant SPLs, significantly negative correlation between ADC value and cell density was also found in other tumors [24][25][26] and in vitro cultured cells 27 .
Animal studies 24,[28][29][30] in other tumors showed that presence of necrosis could reduce cell density and increase water diffusion. Usuda et al. 31 reported contrary result in 63 patients with primary non-small cell lung cancer, however, such analysis is weak if necrosis is not independently correlated with water diffusion. In our study, only positive correlation was found in a small number of squamous carcinoma with no statistical significance. Lyng et al. 24 suggested that necrotic regions should be comparable to or larger than voxel size of DWI so that partial volume effects could be avoided. In our histopathological analysis, necrotic regions with small size (mostly less than 2 × 2 mm size) was distributed diffusely in most (8 of 9) included squamous carcinomas, which may lead to non-significant result in correlation analysis.
Presence of mucus was another independently correlated parameter with water diffusion. To the best of our knowledge, only three studies reported the effect of mucus on ADC value in malignant SPLs [31][32][33] , and one study with more samples showed statistical significance 32 . Contrary to the result of several prior studies [18][19][20]22 , the present results failed to show correlation between grade of lung cancer and ADC value. Significant correlation between grade of differentiation and cell density as reported by Liu et al. 22 might give some explanation to grade of differentiation as confounding parameter.
In terms of the diagnostic capability of ADC value in benign SPLs, we found that it was better in noninflammatory SPLs than in inflammatory SPLs which maybe caused by the overlap of ADC value between active inflammatory lesions and malignant lesions. Ciet et al. 34 reported that ADC value of inflammatory lesion significantly increased after intravenous antibiotic treatment compared with baseline in patients with cystic fibrosis admitted for respiratory tract exacerbations treatment. The repeatability of ADC measurement in our study was better than Bernardin et al. 's 35 as we included more SPLs with size >2 cm or located at upper lobe. We noted that the proportion of excluded participants due to inadequate image quality for analysis was 12.0% (13/108), which was higher than that in prior studies by Yuan et al.  14 . In our opinion, different DWI protocols may lead to potential difference in imaging quality of SPL. Respiratory-triggered gating technique was applied in our DWI sequence and part of prior studies 9,13,31,32 , while free breathing was adopted in others 8,10,14,21,22,35 . One recent study reported that ADC measurement for lung cancer had no significant difference in inter-and intraobserver agreement whatever free breathing, breath-holding or respiratory triggering was used 36 , but small samples (n = 22) and all included lesions were larger than 2 cm limit the statistical power of the result. Further high quality studies are still needed.
This study had some limitations. Firstly, the samples for benign SPLs and some types of malignant SPLs (squamous carcinoma, adenosquamous carcinoma and small cell lung carcinoma, etc) were relatively small. Since only 1 small cell lung carcinoma was surgically excised, comparison between small cell lung carcinoma and non-small cell lung carcinoma could not be performed. With only 9 participants with squamous carcinoma and 5 participants with tumors with mucus, our result on accurate diagnosis rate of ADC value in squamous carcinoma (with 100% accuracy) and tumors with mucus (with 0% accuracy) maybe too optimistic. Difference of diagnostic capability of ADC value among different types of malignant SPLs need to be further validated in studies with large sample size. Also, we did not make a precise correspondence between the site of histopathological analysis and that of ADC measurement. Point to point correspondence is necessary for radiologic-histopathologic comparison so that bias can be reduced, although it is technically difficult.

Conclusions
Cell density and presence of mucus are independently correlated with ADC value in malignant SPLs. Squamous carcinoma maybe more accurately diagnosed as malignancy by ADC value because of higher cell density and lower ADC value. Malignant SPLs with mucus and adenocarcinomas with low cell density should be kept in mind in differentiating SPLs using ADC value because of insufficient diagnostic capability.

Methods
Participants. The prospective study was approved by the Ethic Committee of Shanghai Ren Ji Hospital, and the experimental protocols were performed in accordance with the approved guidelines. Informed consent was obtained from all of the participants before the study began. Patients with SPL (with size ≥ 1 cm, no air-containing area or calcification) found on CT and without resorption after 2 weeks of anti-infective treatment using oral or parenteral antibiotics were included. The exclusion criteria for participants were: (1) in poor physical   T2-weighted spectral presaturation inversion recovery (SPIR) fat suppression: TR/TE 974/65 msec, FOV 250 × 250 mm 2 , matrix 252 × 210, slice thickness/gap 3/0.5 mm, NSA = 2, multi-shot; (4) axial mDixon TSE: TR/TE 3.5/1.2 msec, FOV 400 × 349 mm 2 , matrix 272 × 236, slice thickness/gap 1.75/0 mm, NSA = 1. Since the choice of b value for DWI in SPL has not come to an agreement and different b values were adopted in previous studies, DWI was performed using axial single-shot spin-echo echo-planar imaging (SE-EPI) sequence with multiple b-values (0, 250, 600, 800 and 1000 s/mm 2 ) and following parameters: TR/TE 1044/69 msec, FOV 400 × 352 mm 2 , matrix 132 × 114, slice thickness/gap 3/0.3 mm, EPI factor 57, NSA = 1 for b value being 0 and 250 s/mm 2 , NSA = 2 for 600 and 800 s/mm 2 , NSA = 3 for 1000 s/mm 2 . SPIR fat suppression was used for DWI to reduce chemical shift artifact. Participants were requested to voluntarily suppress their breathing during the scan of mDixon TSE sequence. Respiratory-triggered gating technique was applied in other sequences. The total duration was approximately 15 minutes. Imaging Analysis. After scanning, ADC map of DWI was generated through mono-exponential fitting by total b values as derived from following equation: S b = S 0 exp(−b × ADC), where S b is the signal intensity for a given b factor, and S 0 is the signal intensity without diffusion weighting. The image quality of DWI/ADC maps was assessed by using three grades: grade 1, excellent, well-defined lesion contours, without distortion (due to magnetic susceptibility artifacts) and motion artifacts; grade 2, appropriate for analysis, identifiable lesion contours after referring to T2-weighted image, with moderate distortion or motion artifacts; grade 3, inadequate for diagnosis, obscured lesion contours, severe distortion or motion artifacts. SPLs with image quality of grade 3 were excluded from final analysis. A chest radiologist (J.C) with 15 years of experience in diagnosis of chest imaging, who was blinded to histopathological results, reviewed all images and measured ADC values. With reference to anatomical MR images, region of interest (ROI) was placed on the slice with largest SPL volume and encompassed entire lesion with a little distance from the edge. The mean ADC value in ROI was obtained for analysis. Lesion's location and size (maximum diameter) on corresponding anatomical images were recorded. In order to assess the repeatability of ADC measurement, ADC values of included SPLs were measured again by another trained radiologist (L.L) with 3 years of experience in interpreting chest MRI.
Histopathological Analysis. Surgically excised SPLs were analyzed by a pathologist (D.T) with 10 years of experience in diagnosis of pulmonary pathology. Cell density, NCR, necrotic fraction, presence of mucus and grade of differentiation were quantified as histopathological parameters in the slice with largest volume of specimen. A Leica DM5000 microscope (Leica Instruments Inc, Germany) coupled with a Leica digital sight DFC295 camera were applied for the conversion of histopathological slices to digital images.
Cell density was defined as an average cell count per five high-power fields, and NCR was estimated through dividing the percentage of nuclear area by that of cytoplasmic area 21,37 . For each lesion, five different fields of view (magnification ×400) were chosen randomly and digital images (with resolution of 2048 ×1536 pixels) were captured. ImageJ software package (National Institutes of Health, Bethesda, MD) was used for quantifying cell density and NCR in each field of view, and the average cell density and NCR in all fields of view were calculated. Necrotic fraction, presence of mucus and grade of differentiation were empirically estimated after reviewing the whole slice. Necrotic fraction was defined as the percentage of necrotic area in total lesion area 24 , and was classified into class I (<25%), class II (25% to 50%), class III (51% to 75%) or class IV (>75%). The grade of differentiation in malignant SPLs was categorized into low grade (well-and moderate-differentiation) and high grade (poor-differentiation) according to prior studies 18,19 . Statistical Analysis. Data were presented as mean ± standard deviation (SD), median ± interquartile range (IQR), range or count (percentage) as appropriate. Inter-reader agreement on ADC value was tested by one-way random intra-class correlation coefficient (ICC). Receiver operating characteristic (ROC) curve was used to calculate the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of ADC value in distinguishing between malignant and benign SPLs. In consideration of sample size, we included all SPLs with histopathological diagnosis (surgery or biopsy), distant organ metastasis or follow-up for at least two years without growth in analysis of ROC curve. For SPLs with surgical pathology available, associations between ADC value and multiple histopathological parameters were assessed using multiple linear regression in malignant and benign lesions, respectively. We also evaluated associations between ADC value and single histopathological parameters in different types of malignant SPLs (with sample size equal to or more than five) using Spearman correlation coefficient. On the base of optimal cut-off value according to Youden's index from ROC curve, the type-specific accurate diagnosis rate was calculated retrospectively in different types of malignant and benign SPLs being surgically excised, which was defined as the percentage of malignant lesions with ADC value equal to or less than optimal cut-off value. Accurate diagnosis rate, ADC value and independently histopathological parameters among different types of malignant SPLs were compared by Chi-square test, student's t-test or Mann-Whitney U test as appropriate. Normal or skewed distribution of continuous variables was assessed using Kolmogorov-Smirnov test. The SPSS software (version 22.0, Chicago, IL, USA) was used for statistical analysis. P < 0.05 was considered statistically significant. Data availability. The datasets generated and analysed during the current study are available from the corresponding authors on reasonable request.