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Confocal microscopic analysis of the microfilament configurations from human vitrification-thawed oocytes matured in vitro.

BACKGROUND
The alteration in microfilaments of human oocytes by vitrification has not been understood well.


OBJECTIVE
To evaluate the effect of vitrification on the microfilaments of human in vitro matured oocytes and the time needed for the repair.


MATERIALS AND METHODS
Human in vitro matured oocytes were divided into the control group (Group 1) and the vitrified group. The vitrified oocytes were further divided into four sub-groups that were cultured after thawing for 1 h, 2 h, 3 h and 4 h, respectively (Groups 2, 3, 4 and 5).


RESULTS
The survival rate of oocytes was 87.4% after vitrification and thawing. The percentage of oocytes with a normal configuration of microfilaments in Group 2 and Group 3 was significantly lower than that of the fresh control group (Group 1), whereas the percentage of oocytes with normal microfilament configuration in Group 4 and Group 5 was comparable to the control group.


CONCLUSION
Vitrification alters microfilament structure of oocytes, which takes at least 3h after thawing for the repair and recovery.