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A novel regulatory mechanism for whey acidic protein gene expression.

When primary mouse mammary epithelial cells (PMME) are cultured on a basement membrane type matrix, they undergo extensive morphogenesis leading to the formation of 3-dimensional alveoli-like spherical structures surrounding a closed lumen. We show for the first time that cells cultured on basement membrane-type matrix express high levels of whey acidic protein (WAP) mRNA and secrete the protein into the lumen. The expression of WAP appears to be dependent upon the formation of the alveoli-like spheres: prevention of sphere formation by fixation or drying of the matrix abolishes the expression of WAP. Co-culturing PMME on native and fixed basement membrane matrix indicates that the suppression of WAP expression is dominant, thereby revealing the existence of a diffusible inhibitor(s). The inhibitory activity is present in the conditioned medium of PMME cultured on plastic surface and floating collagen gels, substrata that do not form alveoli and do not allow WAP expression. These findings are consistent with the model that the synthesis, or the action, of the WAP inhibitory factor is regulated by the tissue-like multicellular organization of mammary cells. When PMME do not have correct 3-dimensional structures, one (or more) inhibitor is secreted into the medium which suppresses WAP expression by an autocrine or paracrine mechanism. Nuclear run-on experiments suggest that the suppression of WAP expression is posttranscriptional. These results have obvious bearings on the understanding of the mechanisms by which cell-cell and cell-extracellular matrix interaction regulate tissue specific gene expressions.