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An Antifungal Agent Produced by Streptomyces olivaceiscleroticus, AZ-SH514

This work was carried out in the course of a screening program for specific the bioactive substances that demonstrated inhibitory affects against pathogenic fungi from actinomycetes strains. Forty five Streptomyces strains were isolated from soil sample collected from El-Sharkia governorate, Egypt. All these strains were screened for their antifungal activity against pathogenic fungi. Among the forty five Streptomyces, the broad spectrum strain Streptomyces sp. AZ-SH514 was selected for compound characterization and purification. The nucleotide sequence of the 16s RNA gene (1.5 Kb) of the most potent strain evidenced an 89% similarity with Streptomyces mutabilis, Streptomyces tendae and Streptomyces olivaceiscleroticus. From the taxonomic features, the actinomycetes isolate AZ-SH514 matches with Streptomyces olivaceiscleroticus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces olivaceiscleroticus, AZ-SH514. The active metabolite was extracted using n-Butanol (1:1, v/v) at pH 7.0. The separation of the active ingredient and its purification was performed using both thin layer chromatography (TLC) and column chromatography (CC) techniques. The physico-chemical characteristics of the purified antifungal agent viz. color, melting point, solubility, elemental analysis, spectroscopic characteristics and chemical reactions have been investigated. This analysis indicates a suggested empirical formula of C H NO. 24 19 The minimum inhibition concentrations "MICs" of the purified antifungal agent were also determined. In conclusion, the collected data emphasized the fact that the purified antifungal agent was suggestive of being belonging to 4' phenyl-1-napthyl-phenyl acetamide antibiotic produced by Streptomyces olivaceiscleroticus, AZ-SH514.