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Dominant negative effect of the truncated p110 subunit of phosphatidylinositol‐3 kinase

Effects of p110, the catalytic subunit of PI‐3 kinase, on induction of TPA response element‐driven promoter by EGF was examined. The induction was enhanced by co‐expression of the wild type p110. The truncated p110 mutants containing the binding site for p85 but missing the catalytic activity repressed the induction. A mutant with no binding activity to p85 did not show this effect. These results suggest that PI‐3 kinase is involved in signal transduction of EGF and that the truncated p110s capable of binding to p85 serves as a dominant negative reagent for PI‐3 kinase.