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[Effects of gentamicin on survival and neuritogenesis of cultured mouse spiral ganglion cells].

The effect of gentamicin on survival and neuritogenesis of cultured spiral ganglion cell(SGC) from NIH mouse 1-5 days after birth was observed. Different doses (25, 50 and 100 mg/L) of gentamicin were add to the medium of SGC, and cultured for 14 days. After that, the specimen was fixed with 4% paraformaldehyde and stained with toluidine blue. The amount of survival SGC was counted, and the length of neurite was measured with micrometer under microscope. It was shown that the survival and length of neurite of SGC had no difference between Gentamicin treated groups and control groups, and there were also no difference among 3 gentamicin treated groups. It was suggested that gentamicin had no effect on survival and neuritogenesis of SGC. The effects of aminoglycoside antibiotics on SGC were probably secondary to the injury of Corti's Organ.