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Simultaneous Separation and Determination of Different Polar Flavonoids in Multiflora Fruit by Reverse-Phase High-Performance Liquid Chromatography

Abstract A method for the determination of different polar flavonoids from multiflora fruit by reverse-phase high-performance liquid chromatography (HPLC) is presented in this article. Chromatographic separation of these flavonoids was performed over a Diamonsil C18 column (200 mm × 4.6 mm × 5 µm) using gradient elution with an isopropanol–methanol–water mixture at 25°C, at a flow rate ranging from 0.5 to 1.5 mL/min and detection at 360 nm. The flavonoids could be separated within 6 min; the recovery was between 96% and 104%, and the relative standard deviation was 1.6–2.6%. This method allows rapid detection of flavonoids.