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BACTERIAL AND FUNGAL CONTAMINANTS OF TISSUE-CULTURED 'LAKATAN' BANANA

This study aimed to characterize the bacterial and fungal contaminants of tissue-cultured 'Lakatan' banana (Musa acuminata) during initiation stage. This was conducted at the University of Southeastern Philippines, Tagum-Mabini Campus from October 2015 to February 2016. The sterilized banana explant was placed in a bottle of solidified nutrient agar medium. Detectable bacterial and fungal contaminants were isolated and sub-cultured into the fresh medium two to four days after incubation. The bacterial isolates were cultivated by streaking into fresh culture medium and incubated at