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Estimation of somatic cells in milk by filter-deoxyribonucleic acid method with indole.

Abstract The filter-deoxyribonucleic acid method of estimating somatic cells in milk was modified by indole instead of diphenylamine for development of color. This reduced the time required for development of color from 16 h to 10 min. The correlation coefficient with direct microscopic somatic cell count was .99 with fresh milk. Two-deoxy-D-ribose yielded a linear relationship between concentration and optical density and was useful as a standard. Effectiveness of preservation of milk for the filter-deoxyribonucleic acid method by sodium dichromate and freezing was established.