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Vasopressin‐induced transfer via light vesicles of receptors and water channels from basolateral to apical membrane of toad bladder

Toad bladder epithelial cells were homogenized and fractionated by Percoll density‐gradient centrifugation. Binding of tritium‐labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60‐min) micrsomal supernatant fraction (S). More than two‐thirds of the total receptors were in S. Receptors in SM — but not in S — were tightly coupled to G‐protein as suggested by inhibition of [3H]AVP binding by GTP. GTP‐sensitivity of [3H]AVP binding was not altered by vasotocin (AVT) stimulation, although the distribution of receptors shifted from SM to S.