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A re-assessment of the tricellular region of epithelial cell tight junctions in trachea of guinea pig.

The tricellular region of epithelial tight junctions was previously dismissed as a possible avenue of permeability. One reason was that the two parallel vertical fibers, which penetrate the depth of the tight junction, were apparently cross-linked. Another reason was that the tricellular region of the tight junction is deeper than the adjacent bicellular regions. In the course of several freeze-fracture studies of epithelial tight junctions we have made observations which led us to re-assess the tricellular region as an avenue of permeability. We believe that information from ectoplasmic fracture faces is less subject to artifacts and, in ectoplasmic fracture faces of tricellular regions, cross-linking of the vertical furrows has not been observed. In guinea pig tracheal epithelium the tricellular junction is only about 1 micron deep. Following exposure to cigarette smoke, lanthanum ion staining has been observed in some tricellular junctions. It seems that earlier reasons for dismissing tricellular regions of the tight junction as permeability sites may be insufficient and that there is some evidence to support a role in permeability.