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Safety assessment and evaluation of probiotic potential of Lactobacillus reuteri strains under in vitro conditions

A B S T R A C T In this study safety and probiotic potential of eight representative Lactobacillus reuteri strains isolated from human infant feces (< 3 months) were determined. These L. reuteri isolates were evaluated for their sensitivity to various antibiotics and production of biogenic amines. L. reuteri isolates were found to be sensitive to most of the tested antibiotics and none of the biogenic amines were produced by these isolates. The safety of the eight indigenous L. reuteri isolates was supported by the absence of transferable antibiotic resistance determinants, DNase activity, gelatinase activity and haemolysis. All the L. reuteri isolates showed resistance to gastric juice and bile. In addition, L. reuteri isolates expressed bile salt hydrolase activity, and had ability to assimilate cholesterol in vitro. In vitro adhesion to HT- 29 cells of up to 69% was recorded. These isolates also demonstrated free radical scavenging activity. According to these results, L. reuteri isolates are generally free from virulence traits, have good probiotic potential and may be exploit in dairy industry and probiotic preparations.