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A "double-locked" probe for the detection of hydrogen sulfide in a viscous system.

A novel "double-locked" probe, DCO-H2S-V, was prepared for detecting hydrogen sulfide in a highly viscous system. Experiments demonstrated that only when H2S and a high viscosity environment coexist in a cell, can the probe be activated effectively and emit fluorescence. This has been successfully used for detecting the changes in viscosity and H2S in a Parkinson's disease model, PC-12 cells treated with glutamate.