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Antioxidant, anti-inflammatory, and anti-fibrotic properties of olive leaf extract protect against L-arginine induced chronic pancreatitis in the adult male albino rat

Chronic pancreatitis (CP) is an inflammatory disease affects the pancreas with upcoming fibrosis and notable parenchymal destruction. CP poses a high risk for pancreatic carcinoma. The present study aimed to investigate, for the first time up to our knowledge, the effect of olive leaf extract on L-arginine induced CP with referral to some of its underlying mechanisms. Forty adult male albino rats were divided equally into four groups; control, olive leaf extract treated (200 mg/kg orally once daily), CP group (300 mg L-arginine/100 g body weight intraperitoneally, once daily for 3 weeks then every 3 days for the subsequent 3 weeks), and CP treated with olive leaf extract group. At the end of the experiment, body weight, serum glucose, serum insulin, homeostatic model assessment of insulin resistance (HOMA-IR), serum amylase and lipase as well as tissue superoxide dismutase (SOD), and malondialdehyde (MDA) levels were assessed. Pancreatic tissues were subjected to histological and immuno-histochemical studies. The CP group revealed significant decrease in body weight and increase in serum glucose, serum insulin, HOMA-IR score, serum amylase, and serum lipase levels. Significant increase in MDA level and decrease in SOD level were detected. Marked degenerative changes and fibrosis were detected. Upregulation of alpha smooth muscle actin (α-SMA), transforming growth factor beta (TGF-β), caspase-3, and interleukin-6 (IL-6) immunoreactions were implicated in CP pathogenesis. Olive leaf extract alleviated all the examined parameters via its-antioxidant, anti-inflammatory, and anti-fibrotic properties. Olive leaf extract can protect against CP and restore pancreatic functions.

cancer in CP patients increased almost eightfold [6].
L-arginine is an α-amino acid used in the biosynthesis of proteins [7]. L-arginine plays an important role in cell division, wound healing, removing ammonia from the body, immune function, and the release of hormones. It is important in the regulation of blood pressure [8]. Despite medical treatment, the lethality of severe acute pancreatitis and CP with L-arginine is still high (20%-30%) [9]. When given as intraperitoneal injections, L-arginine is easily administered and damages pancreatic cells. So, it can be used as a method to induce CP by repeated injections over a longer period [10].
In spite of decades of research, knowledge about the specific pathogenesis of CP is still limited. So, the treatment of this disease remains empirical, and the current therapeutic plans of this disease are symptomatic and supportive including nutrition and pain control [11]. However, inflammation and free radicals are forming together the scaffold of disease etiology [12].
Pancreatic stellate cells (PSCs) can regulate the normal architecture of the pancreas by balancing the matrix degradation process and fibrogenesis [13]. An inflamed pancreas induced the PCSs' proliferation and activation with secretion of cytokines and extracellular matrix (ECM) proteins [14]. Activated PSCs have been accepted as a key precursor of pancreatic fibrosis, which is a hallmark of CP. Hence, it is evident that targeting PSCs may be a promising treatment for CP [15].
For a long time, phytotherapy as olive tree has been used to treat many diseases [16]. The main ingredient of olive leaf is oleuropein, which has a high antioxidant activity. Thus, olive leaf is considered one of the most active natural antioxidants [17]. In addition, the olive leaf contains the highest concentration of olive plant polyphenols more than the fruit or oil [18]. Olive leaf extract (OLE) is used as a supplementation or as a drug. It has many benefits including anti-atherosclerotic, anti-arrhythmic [19], anti-tumor, antihypertensive, antioxidant [20], anti-inflammatory [21], and anti-fibrotic [22] properties. Thus, the research community's interest in olive leaves and their extracts has intensified in recent years because of the olive's great potential for use in medicine and the pharmaceutical industry [23].
Taken together, this study was designed to examine, for the first time up to our knowledge, the usefulness of OLE administration in preventing L-arginine induced CP in a rat model.

Animals
The current study was conducted on 40 healthy adult male Sprague-Dawley rats (3 months old, weighing 180-200 g). They were kept in the animal house of the Faculty of Medicine, Menoufia University, Egypt. The animals were maintained under controlled temperature and humidity conditions and provided with water and a balanced diet ad libitum. Strict care and hygiene measures were taken to maintain a healthy environment for all rats all the time. All experimental procedures were approved by the ethical research committee of the Menoufia University Faculty of Medicine, Egypt (MU29122020).

L-Arginine
It was purchased from Sigma Chemical Co. (St. Louis, MO, USA) in the form of powder and dissolved in normal saline (40% wt/vol, pH 7.4) [24].

Olive leaf extract
The leaves of olive (Olea europaea L.) were collected from Al Arish, Egypt. The plant material was authenticated in the Faculty of Agriculture, Menoufia University, Egypt. The extract was prepared at the Faculty of Science, Menoufia University, Egypt using the method of Al-Attar and Abu Zeid [25] with some modifications. The powder of dried olive leaves (200 g) was prepared and added to 7 L of hot water. After 3 hours, the mixture was slowly boiled for 30 minutes. The mixture was then cooled to room temperature and subjected to an electric mixer for 20 minutes. Thereafter, the solution was filtered. The filtrate of olive leaves was evaporated in an oven at 40°C to produce dried residues (active principles). The yield mean of the leaves extract was 20.3% with references to the powdered samples.

Experimental design
After an adaptation period of one week, rats were randomly divided into four equal groups (ten rats each): • Group I (control group): kept without any treatment.
• Group II (OLE treated group): received a daily oral dose of aqueous OLE by gastric intubation at a dose 200 mg/ kg, dissolved in 2 ml distilled water, for 10 weeks [26]. • Group III (CP group): received distilled water, in an Anat Cell Biol 2022;55:205-216 207 www.acbjournal.org amount equal to that used for dissolving OLE, for 4 weeks to be followed by CP induction by intraperitoneal injection of 300 mg L-arginine/100 g body weight, dissolved in normal saline (40% wt/vol, pH 7.4), once daily for 3 weeks. Subsequently, L-arginine injections were given at the same dose every 3 days for the subsequent 3 weeks [24]. • Group IV (CP treated with OLE group): received OLE, at the same dose and route of administration, 4 weeks before L-arginine injection. Supplementation with OLE continued during the period of CP induction for further 6 weeks.

Specimen collection
At the end of the experimental period (10 weeks), the rats' body weight was estimated, and the blood samples were collected from the rats' tail dorsal veins for the biochemical evaluation. Rats were anaesthetized using an intraperitoneal injection of ketamine (90 mg/kg)/xylazine (15 mg/kg) and then sacrificed by cervical dislocation. The anterior abdominal wall was incised longitudinally, and the pancreas was dissected out and flushed with physiological saline. Each specimen was divided into two portions; one was fixed in 10% neutral buffered formalin for the histological and immunohistochemical assessment, and the other was immediately frozen at −70°C to be used for tissue biochemical evaluation.

Biochemical study
Estimation of blood glucose & serum parameters: Fasting blood glucose level was evaluated using blood glucose test strips (Accu-Chek; Diagnostic Solutions Inc., Irvine, CA, USA) and One Touch Basic Blood Glucose Meter (Life Scan Inc., Milpitas, CA, USA). The other blood samples were allowed to clot then centrifuged at 4,000 rpm for 10 minutes. The collected serum was used for assessment of serum levels of insulin [27], amylase [28], and lipase [29].
Assessment of lipid peroxidation & tissue antioxidant status: The frozen pancreatic tissue was homogenized in icecold phosphate buffer (140 mmol/L KCl, 20 mmol/L phosphate, PH 7.4) and centrifuged at 4,500 rpm for 15 minutes at 4°C. According to Kono [30], the supernatant was used to measure the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the tissues.

Histological study
After fixation in 10% neutral formalin for 24 hours, the pancreatic specimens were dehydrated using ascending grades of alcohol then cleared and embedded in paraffin. Microtome was used to cut sections of 5 µm thick. Sections were subjected to hematoxylin and eosin (H&E) staining for routine histological examination and Mallory trichrome stain for detection of collagen fiber deposition.
Histologic scoring of area of lesions, inf lammation, and fatty infiltration were performed by Image J software (Bethesda, MD, USA) in at least three sections/animal according to Liu et al. [31] (Table 1).
For immunohistochemical staining, paraffin sections were placed in xylene for 1-2 minutes, then rehydrated with degraded ethanol (100%, 95%, and 70% ethanol) twice for 5 minutes each time, and then inserted in distilled water for another 5 minutes. The slices were inserted in phosphate buffered saline (PBS) and blocked in 0.1% H 2 O 2 for 30 minutes to impede the endogenous peroxidase activity. The sections were then maintained in a blocking solution (10% normal goat serum) at room temperature (22°C) for 60 minutes. Then incubation with the primary antibodies: caspase-3 (rabbit polyclonal antibody, 1:400; Abcam, Cambridge, MA, USA), interleukin-6 (IL-6) (a mouse monoclonal antibody, 1:400; Abcam), alpha smooth muscle actin (α-SMA) (a rabbit polyclonal antibody, 1:100; Abcam), and transforming growth factor beta (TGF-β) (a mouse monoclonal antibody, 1:500; Midco Trade) was performed. After PBS, the sections were incubated with biotinylated secondary antibody for 20 minutes at room temperature. After rinsing with PBS, streptavidin-Horseradish peroxidase solution was placed on the slices for 10 minutes; 3,3-diaminobenzoic acid dissolved in PBS was used for secondary antibody binding; and 0.03% H 2 O 2 was added immediately before use. Finally, the sections were dipped with PBS, and the slides were counterstained with two drops of hematoxylin. The slides were then dehydrated with ascending ethanol (70%, 95%, and 100%) for 5 minutes, then cleared with xylene and then covered with coverslips using a mounting solution [32].

Quantitative assessment
Using Image J software version K 1.45, the following parameters were measured: 1. The area % of collagen deposition. 2. The area % of caspase-3, IL-6, α-SMA, and TGF-β immunoreactions. For each parameter, five non-overlapping fields (×40) were randomly taken from every specimen using Leica DML B2/11888111 microscope equipped with Leica DFC450 camera.

Statistical analysis
The collected data were expressed as the mean±SD. The data was analyzed using SPSS version 23 (IBM Corp., Armonk, NY, USA). One-way analysis of variance (ANOVA) followed by Bonferroni post-hoc test was used to assess the significance of differences between groups. When P-value <0.05, the result was considered statistically significant [33].

Results
There was no significant difference in all the examined parameters between the control and OLE treated groups.

Body weight results
At the end of the experiment, there was a significant decrease (P<0.001) in the body weight in the CP group

Biochemical results
Compared with the control group, the blood glucose level of the CP group increased significantly (P<0.001) (133.33±4.16 vs. 91.05±2.73). Compared with the untreated CP group, this level was significantly reduced (P<0.001) in the CP group treated with OLE (95.67±3.48 vs. 133.33±4.16) (Fig. 1B).
Regarding the serum insulin level, a significant increase (P<0.001) in its level was detected in the CP group (21.24±2.24 vs. 9.38±0.301) compared with the control group, while the CP group treated with OLE exhibited a significant decrease (P<0.001) in the serum insulin level (10.31±0.461 vs. 21.24±2.24) compared with the untreated CP group (Fig.  1B). So, there was a significant increase (P<0.001) in HOMA-IR score (insulin resistance) in the CP group (6.77±0.302 vs. 1.24±0.168) compared with the control group. But HOMA-IR score in the CP group treated with OLE exhibited a significant decrease (P<0.001) compared with the untreated CP group (2.01±0.213 vs. 6.77±0.302) (Fig. 1B).
The serum amylase and lipase levels showed a significant increase (P<0.001) in the CP group compared with the con-    Fig. 2A, B). The assessment of MDA and SOD levels in the pancreatic homogenates revealed a significant increase (P<0.001) in MDA level (13.08±0.292 vs. 1.169±0.067) and a significant decrease (P<0.001) in SOD level (0.694±0.055 vs. 2.409±0.298) in the CP group compared with the control group. In addition, the CP group treated with OLE showed a significant decrease (P<0.001) in the MDA level (6.84±0.58 vs. 13.08±0.292) and a significant increase (P<0.001) in SOD level (1.52±0.14 vs. 0.694±0.055) when compared with the untreated CP group (Fig. 2C, D).

Histological results
Light microscopic study H&E stained sections from the control group showed that the pancreas was divided into many small lobules of different sizes and shapes, separated by interlobular connective tissue. Each lobule consisted of the main exocrine part; a compound duct system and pancreatic acini with basal basophilic part and an eosinophilic apical part. The acinar cells had vesicular nuclei with prominent nucleoli. Masses in the endocrine part (islets of Langerhans) were unevely distributed in-between the pancreatic acini ( Fig. 3A-C).
The CP group showed marked degenerative alterations. Most of the acinar cells showed vacuolation of the cytoplasm and decreased zymogen granules. Dilatation of the interlobular pancreatic duct was observed. Dilated congested blood  vessels were also seen. Inflammatory cellular infiltration among the degenerated pancreatic acini was seen. Islets of Langerhans showed many vacuoles and most of the cells appeared with deeply stained pyknotic nuclei ( Fig. 3D-F). The CP group treated with OLE showed an improvement of most of the previously mentioned histopathological findings in the CP group. The pancreatic lobules appeared almost normal. The majority of the acinar cells appeared nearly normal, while some cells appeared with deeply stained pyknotic nuclei. Islets of Langerhans were almost normal, but some vacuoles and deeply stained pyknotic nuclei were also seen ( Fig. 3G-I).
Statistically, the scores of area % of lesions, fat infiltration and inflammation were significantly increased (P<0.001) in the CP group compared with the control group (2. showing normal pancreatic acini "A" with vesicular nuclei (arrows) and normal islets of Langerhans "i". Normal intralobular pancreatic duct "D" is seen. (D-F) CP group exhibiting marked vacuolations "V" and pyknotic nuclei (arrowhead) of the islets of Langerhans "i" and disorganized pancreatic acini (asterisk). Congested blood vessel "BV", inflammatory infiltrate (thick arrow), thick walled (curved arrow) intralobular pancreatic ducts "D", decreased zymogen granules (asterisk) and fatty degeneration "F" are seen. (G-I) CP group treated with OLE revealing regaining of nearly normal β-cells (arrowhead), but pyknotic nuclei (arrow), islets of Langerhans "i", and some vacuoles (curved arrow) can be also seen. Restored pancreatic acini "A" with many vesicular nuclei (arrow) except for some pyknotic nuclei (curved arrow) are seen; Restored intralobular pancreatic duct "D" is also noted. CP, chronic pancreatitis; OLE, olive leaf extract.
Anat Cell Biol 2022;55:205-216 211 www.acbjournal.org spectively) (Fig. 4). The Mallory trichrome stained sections of the control group showed slight collagen deposition between the pancreatic lobules and in the islets of Langerhans (Fig. 5A, B). Sections of the CP group showed excessive collagen deposition around the ductal system and between the degenerated pancreatic acini. In addition, islets of Langerhans showed a large amount of collagen fiber deposition (Fig. 5C, D). The sections of the CP group treated with OLE showed fine collagen fibers between the pancreatic lobules, around the duct system, and in the islets of Langerhans (Fig. 5E, F).
Statistically, a significant increase (P<0.001) in the area % of collagen deposition in the CP group compared with the control group (98.08±1.32 vs. 0.686±0.06). In addition, there was also a significant decrease (P<0.001) in the area % in the CP group treated with OLE compared to the untreated CP group (36.55±3.06 vs. 98.08±1.32) (Fig. 5G).

Discussion
Considering the growing incidence of pancreatitis and inflammatory pancreatic disorder as well as life-changing processes that affect patients with CP, it becomes highly necessary to search for effective methods to prevent and mitigate the disease [34]. The use of natural agents, particularly plant-based drugs, is favored for preventing and/or improv-  ing CP compared to other complementary and alternative approaches [35]. This study revealed, for the first time to our knowledge, that OLE is an effective natural agent in preventing CP induced by L-arginine in an experimental rat model. Interestingly, rats treated with OLE showed no toxicity to the pancreas from the serum enzyme assay and morphological studies, showing that OLE is a relatively safe agent for the long-term administration. In this study, the CP model was induced by daily injection of L-arginine followed by intermittent injections. At the end of the experiment, although the pancreatic tissues of the CP and the CP+OLE groups were different in extension, they showed histopathological features of CP such as pancreatic fibrosis and atrophy of the pancreatic exocrine cell mass. So, CP induction was successful in this experimental model. This was supported by Al-attar and Alsalmi [26] who stated that this protocol was a reliable method for inducing CP.
They mentioned that daily injection of L-arginine produced reproducible lesions similar to human CP, and those intermittent injections were required to maintain them.
Moreover, significant elevation of serum levels of the digestive pancreatic enzymes, amylase and lipase, was detected in this study. This further confirmed that CP was developed in this experimental rat model as previously reported by Khan and Matar [36] who stated that these two enzymes' levels, in experimental studies, are important markers in establishing the efficacy of investigational agents for development of pancreatitis. This could explain weight loss observed in the CP group as a result of the decreased absorption of essential substances in the ingested food caused by the deteriorated function of the exocrine pancreas [37]. OLE supplementation could significantly reduce such elevated activities of these enzymes, suggesting that OLE possibly has a certain degree of protective influence against L-arginine induced www.acbjournal.org pancreatitis and altered pancreatic acinar cell functions [38]. Pancreatic fibrosis is a very important histological finding of pancreatic diseases, such as CP, leading to progressive loss of the lobular morphology and architecture of the pancreas, damage of the large ducts, and severe changes in the arrangement and structure of the islets of Langerhans as previously reported by Bombardo et al. [39]. In this work, similar findings were detected in pancreatic specimens of CP which further supported the development of the disease. Sections of the pancreas from the OLE treated group, on the other hand, showed restored tissue architecture with a significant reduction in fibrosis, suggesting that OLE might have a direct effect on the pancreas in ameliorating CP injuries. Moreover, the antifibrotic effect of OLE was previously reported in the liver of cadmium intoxicated rats [20]. Geyikoglu et al. [40] explained that OLE bioactive constituents such as oleuropein and hydroxy-tyrosol reversed fibrotic and collagen deposition via anti-inflammatory and antioxidant mechanisms. The appearance of fat droplets in the acinar cells was an indicative criterion for the development of pancreatitis in rats [41].
Fibrosis is a marked sign that interstitial PSCs have been activated in CP due to the upregulation in the release of mast cell degranulation and lipid peroxidation products [39]. TGF-β is a major pro-fibrogenic cytokine that was significantly increased in the pancreatic specimens of CP in this study. Increased expression of TGF-β has been observed in the damaged acinar cells adjacent to areas of fibrosis [42]. TGF-β upregulated in CP specimens, promotes PSC activation, and stimulates ECM synthesis [43,44]. Moreover, the expression of α-SMA, which indicates the activation of PSCs, was significantly upregulated in CP in this work as well as in previous studies [45,46]. OLE administration led to marked reduction of TGF-β and α-SMA expression, implying that targeting the PSCs could be one of the important mechanisms for the anti-fibrogenic effect of OLE. In accordance with this, rhein and ellagic acid, phytochemicals extracted from plant-based foods, treatment deactivated PSCs by reducing the expression of their fibrogenic markers α-SMA and TGF-β which are associated with pancreatic fibrosis [34].
Decreased antioxidant levels and increased oxidative stress play key roles in the pathogenesis of CP [47]. Significant oxidative stress and lipid peroxidation were detected in our animal model of CP as manifested by the decrease of SOD and increase of MDA level as well as caspase-3 expression. Increased reactive oxygen species (ROS) production can lead to damage of the cellular macromolecules such as proteins, DNA and lipids producing lipid peroxidation [48]. Treatment of the rats with OLE significantly attenuated CP associated oxidative stress and lipid peroxidation. This antioxidant activity has been reported in multiple studies [49][50][51]. According to recent reports, apart from their antioxidant properties, polyphenols have been found to have far-reaching modulatory effects on key mitochondrial pathways thus protecting the cell from the toxic effects of ROS [52]. The anti-apoptotic effect of OLE was confirmed by the declined expression of caspase-3 in the pancreas of CP rats. This reproduced the results of previous research [17].
The underlying inflammation of the pancreatic parenchyma and the associated subsequent fibrotic destruction are common pathogenic factors in CP and represent targets for protection by modulating the pancreatic inflammation [53,54]. Laboratory research emphasized that the proliferation of ROS is a trigger for inflammation in CP because they activate the signaling cascades that convert the highly damaged acinar cells into chemokines and cytokines production sites [55]. This was consistent with the results of this study, which revealed inflammatory cell infiltration and significant upregulation of IL-6 expression in CP specimens. After oral administration of OLE, this inflammation was significantly reduced. Based on the results of this study, the anti-inflammatory effects of OLE and its bioactive ingredients have been reported in many studies [56,57].
In the current work, significant increase in plasma glucose levels associated with hyperinsulinemia in rats with CP indicated insulin resistance (IR) which was confirmed by the significant upregulation of HOMA-IR. Eriksson [58] postulated that excessive ROS production led to IR by reducing the insulin signaling pathway. Hennig et al. [59] stated that phosphorylation of insulin receptor substrate following the increase of proinflammatory cytokine production, causing antagonistic properties in insulin, could lead to insulin resistance. The antioxidant and anti-inflammatory properties of OLE could thus explain the improved IR, hyperglycemia and hyperinsulinemia associated with extract administration in this work. de Bock et al. [18] reported similar effects of OLE and confirmed that OLE could attenuate IR by reducing the expression of tumor necrosis factor-α, IL-6, and IL-1β while improving insulin signal transduction to lower blood glucose levels.
Summing up our findings, we can conclude that olive leaf extract could effectively prevent CP and restore pancreatic www.acbjournal.org https://doi.org/10.5115/acb. 21.187 functions by attenuating oxidative stress, fibrosis, inflammation and apoptosis and enhancing the antioxidant defenses. From a future perspective, further studies can be applied on animals with fully developed pancreatitis to prove its curative effect. Moreover, further detailed experiments and clinical trials will be required before establishing the potential clinical application of olive leaf extract.