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Development and application of immobilized surfactant in mass spectrometry-based proteomics

In “bottom-up” proteomics, proteins are first digested into peptides and then characterized by chromatographic separation and mass spectrometry analysis, in which proteolysis has an appreciable influence on the repeatability and reliability of analytical results. To improve recovery and enzymatic efficiency, proteins are often denatured and solubilized with chemical agents such as sodium dodecyl sulphate (SDS), urea (UA), etc. However, surfactant like SDS is difficult to remove from the reacting system and may interfere with protease activity, chromatographic separation of peptides and mass spectrometry analysis. To this end, we have prepared an immobilized surfactant which can preserve the solubilization and denaturation abilities of a surfactant and has the advantage of being easily separated from samples. The denaturation and solubilization capabilities of the newly developed immobilized surfactant were evaluated by comparing the enzymatic hydrolysis efficiencies in the pretreatment of protein samples treated with immobilized surfactant and other common methods. The results showed that the average protein sequence coverage was 42.1% (digested for 1 h), which was 76.89% higher than that of a control experiment (23.8%) in the digestion of a standard protein (BSA) and reached the same denaturation ability as that of heating at 95 °C. Moreover, the immobilized surfactant was further tested in the extraction and digestion of the total protein of HeLa cells. The number of identified proteins resulted in similar levels to those exploited with 0.1% SDS and 8 M UA, both of which are frequently used in lysis buffer, indicating that the immobilized surfactant had a good ability to denature and solubilize proteins. In brief, the immobilized surfactant avoids the shortcomings of UA and SDS and can be utilized in the pretreatment of complex biological samples.


Introduction
Proteomics is the large-scale study of proteins, particularly their amino acid sequences, post-translational modications, structures and functions, expressed from a genome, organism, tissue, or cell type. [1][2][3] Genes are carriers of genetic information, and proteins are carriers of life activity. The human genome project, which began in the 1990s, has made great advancements. Life science has entered the post-genome era, and the focus has shied from revealing the genetic information to exposing the laws of life activity at the molecular level, in which proteomics is one of the most important components. [4][5][6] The main research strategies of proteomics include "bottom-up", "middle-down" and "top-down". [7][8][9] In bottom-up proteomics, also called "shotgun" proteomics, 10 proteins are rst digested into peptides, followed by reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The resulting MS data are searched against a protein sequence database for peptide identication; then, the corresponding proteins can be inferred. [11][12][13] The "bottom-up" proteomic strategy is widely used in protein expression proles, post-translational modications, protein interactions and proteome quantication. [14][15][16] However, there are still many difficulties involved when utilizing this strategy, such as obtaining deep coverage, reliability, and repeatability due to the variable physical and chemical properties resulting from differences in amino acid sequences, the varying abundance of different proteins, and the complexity of post-translational modications, spatial structure and proteinprotein interactions. 17,18 Therefore, it is necessary to develop new technologies to improve proteomics methods. [19][20][21][22] The pretreatment conditions of protein samples have a signicant effect on the composition, chromatographic separation and mass spectrometry analysis of peptide mixtures. 23 Improving recovery and enzymatic efficiency is of great importance for the reproducibility and reliability of proteomic analysis. 24 Surfactants are commonly used reagents in sample pretreatment and are applied to denature and solubilize proteins, thereby increasing the enzymatic efficiency of the digestion enzymes acting upon the protein sample. Sodium dodecyl sulfate (SDS) is an effective and popularly used surfactant. 25,26 However, SDS has many disadvantages, including reducing protease activity, thereby affecting enzymatic efficiency, interfering with chromatographic separation and suppressing peptide mass spectrometry signals. Therefore, it oen be removed from the system before enzymatic digestion and LC-MS/MS analysis. Conventional sample purication methods, such as precipitation, extraction, ion exchange and dialysis cannot completely remove the SDS in samples, and the removal process can cause an excessive loss of proteins and peptides. New reagents have been utilized to improve the solubilization process, such as sodium deoxycholate (SDC) 27 and ionic liquids, 28 which are easier to remove and have better mass spectrometric compatibility. In addition, the lter-aided sample preparation (FASP) method 29 and desalting columns 30 are also used for removal of SDS. However, these methods suffer from cumbersome and timeconsuming steps, and need to be further improved.
In recent years, magnetic nanoparticles have been widely used as a new biofunctional material 31 due to their excellent biocompatibility, low toxicity and large surface area. 32,33 The easy preparation and rapid magnetic response make magnetic nanoparticles an ideal carrier material for use in uorescence imaging, drug delivery and proteomic research. [34][35][36] In this study, we prepared surface-active surfactant immobilized magnetic nanoparticles that preserve the solubilization and denaturation abilities of a surfactant and have the advantage of easy magnetic separation. Thus, no surfactant remains in the sample during proteolysis, which has no negative effect on protein hydrolysis, chromatographic separation or mass spectrometry analysis.

Syntheses of Fe 3 O 4 nanoparticles and Fe 3 O 4 @TMOS material
First, 1.46 g ferric chloride hexahydrate and 0.5 g ferrous chloride tetrahydrate were dissolved in 6.3 mL of 0.4 M chlorine.
Next, 62.5 mL of 0.7 M ammonia was added to a round bottom ask and stirred vigorously. The hydrochloric acid mixture above was added to the round bottom ask rapidly and stirred for 1 h at room temperature. The precipitation was separated by means of a magnet, washed three times with ethanol and dried under vacuum at 60 C, by which we obtained ferroferric oxide (Fe 3 O 4 ) nanoparticles.
Fiy milligrams Fe 3 O 4 nanoparticles, 22.857 mL ethanol, 5.714 mL water and 0.214 mL ammonia were added together and sonicated for 30 min. The mixture was then transferred to a 100 mL round bottom ask, and 0.143 mL tetraethyl orthosilicate was added dropwise and then stirred for 6 h at room temperature. The precipitate was washed three times with isopropanol.
The resulting material was dispersed in 8.571 mL isopropanol, 259 mL TMOS was added dropwise under N 2 atmosphere, and the mixture was stirred for 24 h at room temperature. The precipitate Fe 3 O 4 @TMOS, the nal product, was washed three times with isopropanol and stored in 3 mL (Fe 3 O 4 @TMOS with a nal concentration of 15 mg mL À1 ) isopropanol.

Pretreatment of proteins
Tryptic digestion of BSA: 1 mg BSA was dissolved in 10 mL 50 mmol NH 4 HCO 3 , with a nal concentration of 0.1 mg mL À1 . Four aliquots of 100 mL BSA solution were denatured separately as described in Table S1. † Trypsin was added to the BSA solutions (enzyme to protein weight ratio of 1 : 100), and incubated at 37 C for 1 h. The solutions were then analysed by MALDI-TOF mass spectrometry by rst depositing 1 mL digested peptide solution onto a MALDI plate, followed by 1 mL CHCA matrix aqueous solution.
Extraction and trypsin digestion of HeLa proteins: four plates (25 mm Â 150 mm, 2 Â 10 7 cells per plate) of HeLa cells were separately centrifuged at 500 Â g for 3 min in four different 1.5 mL EP tubes, and the supernatant was discarded. One millilitre of the different lysis buffers, as shown in Table S2, † was added to each of four EP tubes along with protease inhibitor. One hundred micrograms of the resulting protein from each method was then reduced with 10 mM DTT at 56 C for 1 h and alkylated with 50 mM IAA at room temperature in the dark for 1 h. Trypsin was then added to the protein solution at a ratio of 1 : 50 and incubated at 37 C for 16 h. The digested peptides of HeLa proteins were desalted using a self-packed C18 desalination column. Of the resulting sample, 1 mg peptides from each lysis method were taken for further HPLC-MS/MS analysis.

Mass spectrometry analysis
MALDI-TOF MS analysis: the MALDI-TOF mass analysis was performed on an ultraeXtreme time-of-ight mass spectrometer (Bruker, Germany) in a positive-ion reector mode with an available accelerating voltage of 20 kV. The nitrogen laser was 337 nm, and the laser energy was 80%. The mass range of the mass spectra was between m/z 700 and 3500.
Nano LC-MS/MS analysis: the high-performance liquid chromatograph used was UltiMate 3000 RSLCnano (Thermo Fisher Scientic, USA). The chromatographic column was selfpacked (150 mm Â 12 cm, New Objective, USA) with a particle size of 1.9 mm (Dr Maisch Gmbh, Germany). The digested peptide mixture was dissolved with mobile phase A (0.1% FA aqueous solution), the loading volume was 5 mL, and the ow rate was 600 nL min À1 . A 90 minute gradient was established with mobile phase A and mobile phase B (0.08% FA-80% ACN aqueous solution). The elution gradient was composed of 0 min of 5% B, 0-8 min of 5% B-8% B, 8-24 min of 8% B-13% B, 24-60 min of 13% B-28% B, 60-79 min of 28% B-40% B, 79-80 min of 40% B-95% B, 80-86 min of 95% B-6% B, and 86-90 min of 6% B. The eluted peptides were passed through an electrospray ionization source (ESI) directly into the mass spectrometer. The mass spectrometer (Q Exactive HF, Thermo Fisher Scientic, USA) was operated in positive mode with a spray voltage of 2 kV. The full MS scan was acquired from m/z 300 to 4000 with a resolution of 120 000 and an MS AGC of 2e6. The MS/MS scan was acquired from m/z 300 to 1400 with a resolution of 15 000, MS2 AGC of 2e4 and MS2 maximum ion implantation time of 19 ms.

Data analysis
MALDI-TOF spectra were analysed by exAnanlysis soware (version 3.3). Peptide mass ngerprints were searched via Mascot (version 2.3.01, Matrix Science). The Mascot parameters were set as follows: the enzyme was set as trypsin, and two missed cleavages at most were allowed; xed modication was carbamidomethylation (Cys), and variable modication was methionine oxidation (Met); and the mass tolerance was set as 0.2 Da.
The raw data of MS spectra was searched with Mascot builtin Proteome Discoverer soware (version 2.1 SP2, Thermo Scientic). The Mascot parameters were set as follows: the enzyme was set as trypsin, and two missed cleavages at most were allowed; xed modication was carbamidomethylation (Cys), and variable modication was methionine oxidation (Met); the precursor tolerance was 10 ppm; the fragment tolerance was 20 mmu; and the false discovery rate (FDR) was set as 1%. The database used was the human protein database (download from http://www.uniprot.org/uniprot/? query¼homo%20protein&l¼&sort¼score on April 18 th , 2017).

Preparation and characterization of the immobilized surfactant
The preparation process of the immobilized surfactant is shown in Scheme 1, in which Fe 3 O 4 nanoparticles with a diameter of approximately 10 nm were rst prepared and then coated with tetraethyl orthosilicate. Finally, the long carbon chains were xed by Si-O to the surface of the magnetic nanoparticles. The denaturation mechanism of Fe 3 O 4 @TMOS was similar to that of a surfactant, mainly through the long carbon chains interacting with proteins and other ends interacting with water to denature and solubilize proteins. The large nanoscale hydrophilic core of Fe 3 O 4 @TMOS remained outside the proteins which was analogous to the hydrophilic side of a surfactant, but unlike the surfactant, Fe 3 O 4 @TMOS can be easily separated from samples under magnetic led. The frequently used Fe 3 O 4 nanoparticles have a larger diameter (approximately 250 nm), such as Fe 3 O 4 nanoparticles prepared by Hu, etal. 37 which carried immobilized enzymes. As proteins in aqueous solution are usually 5-10 nm in diameter, Fe 3 O 4 nanoparticles with larger sizes cannot easily make full contact with proteins. Therefore, a coprecipitation method was used to prepare smaller and more uniform Fe 3 O 4 nanoparticles. In addition, the magnetic nanoparticles had numerous hydroxyl groups aer coating with SiO 2 this endows good hydrophilicity for the hydrophilic side. The long carbon chain with hydrophobicity, as the hydrophobic side, can insert into proteins and induce protein denaturation and solubilization.
The morphology of Fe 3 O 4 and Fe 3 O 4 @TMOS nanoparticles was characterized by transmission electron microscopy (TEM, FEI, USA). As shown in Fig. 1, the Fe 3 O 4 nanoparticles have an average diameter of 10 nm. Aer modication, the edges of the Fe 3 O 4 @TMOS nanoparticles were obviously blurred, and the size was also marginally increased (Fig. 1e). Since Fe 3 O 4 @TMOS had a magnetic response and a small particle size, there was a tendency for it to agglomerate. However, it can be uniformly dispersed in the solution by ultrasound before use (Fig. 2a).
According to elemental analysis of the Fe 3 O 4 and Fe 3 O 4 @ TMOS nanoparticles (Table 1), aer modication with TMOS, the carbon content increased from 0.81% to 3.44%, which indicates that TMOS was successfully immobilized on the surface of Fe 3 O 4 nanoparticles. Fourier transform infrared  spectroscopy (FT-IR, Nicolet, USA) was used to further characterize the chemical constituents of these two types of nanoparticles. As shown in Fig. 3, TMOS showed strong peaks at 2920 cm À1 and 2860 cm À1 , which are the absorption peaks of C-H stretching vibrations (s C-H ). These peaks were not observed in the FT-IR spectra of Fe 3 O 4 nanoparticles, while aer immobilization of TMOS on the surface, these peaks could again be clearly identied. The results suggest that TMOS was successfully immobilized on the surface of the nanoparticles. The amount of TMOS bound to the surface of Fe 3 O 4 @TMOS was measured by thermogravimetric analysis (TGA, TA instrument, USA). As shown in Fig. 4, the heat loss of Fe 3 O 4 nanoparticles was approximately 8%, which was due to the volatilization of the crystallization water, consistent with the results of elemental analysis, which showed that Fe 3 O 4 nanoparticles contained 1.373% elemental hydrogen. In contrast, the weight loss of Fe 3 O 4 @TMOS was up to 17%, which was due to the volatilization of crystallization water and carbon chains on the surface of Fe 3 O 4 @TMOS under high temperature conditions.
The amount of TMOS bound to the surface of Fe 3 O 4 was found to be 160 mg mg À1 . As shown in Fig. S1, † the magnetic eld lines are uniformly symmetrical in the magnetic hysteresis loop (Quantum Design, USA), indicating that the prepared Fe 3 O 4 nanoparticles had superparamagnetism. Aer modication of the carbon chain, the magnetic properties of Fe 3 O 4 @TMOS were not signicantly reduced, indicating that Fe 3 O 4 @TMOS had constant magnetic properties and could be easily separated under an applied magnetic eld (Fig. 2a and b).

Protein denaturation ability of the immobilized surfactant on standard protein BSA
SDS is difficult to be removed from samples and interferes with the ionization behaviour of peptides when analysed by mass spectrometry. Fig. 5 shows that the MALDI-TOF mass spectra of standard protein BSA digests cleaved by trypsin for 1 h under the following conditions: (Fig. 5a) undenatured, (Fig. 5b) denatured with SDS followed by removal of SDS using the FASP method, (Fig. 5c) denatured with SDS without SDS removal, (Fig. 5d) denatured at 95 C and (Fig. 5e) denatured with Fe 3 -O 4 @TMOS. Compared with Fig. 5a, the normal peptide peaks were absent and a large number of impurity peaks were apparent in the sample denatured with SDS (Fig. 5c). The result indicated that SDS had a considerable interference with mass spectrometry analysis and could not be removed by the conventional FASP method (Fig. 5b). Thermal denaturation, a frequently used denaturation method (also a completely denatured method), (Fig. 5d), showed that the number of peptide peaks increased compared with undenatured sample (Fig. 5a). Using the Fe 3 O 4 @TMOS denaturation method, the number of peptide peaks was signicantly increased, and no impurity peaks appeared (Fig. 5d). The results indicate that Fe 3 O 4 @TMOS can denature BSA effectively and can be completely separated by an external magnetic eld and these nanoparticles do not interfere with the mass spectrometry analysis.
The amino acid sequence coverages of BSA digested under different denaturing conditions were examined. As shown in    Table S3-S5 † and Fig. S2-S4, † the 41% amino acid sequence coverage of BSA digestion aer denaturing by calefaction at 95 C indicated that the BSA was completely denatured. Coverage had been greatly improved compared to the results of undenatured BSA of 23.8%. In addition, no peptides can be identied in BSA digests denatured by SDS because of the interference by SDS in mass spectrometry analysis. As shown in Fig. 6, the average amino acid sequence coverage of BSA digested aer denaturation by Fe 3 O 4 @TMOS for three times was 42.1%, which was much higher than that of the undenatured BSA digest (increased by 76.89%) and reached the same enzymatic efficiency of calefaction at 95 C (Fig. 6). Therefore, Fe 3 O 4 @TMOS can denature BSA completely, indicating that Fe 3 O 4 @TMOS has a substantial ability to denature protein.

Examination of protein denaturation and solubilization abilities of the immobilized surfactant using a complex sample
To further examine the protein extraction, denaturation and solubilization performance of the immobilized surfactant, a complex sample, HeLa cells, was processed with the immobilized surfactant and compared with extraction by H 2 O, 0.1% SDS and 8 M UA ( Table 2). The ability of Fe 3 O 4 @TMOS to extract proteins from a complex sample was demonstrated by comparing the results to those of using H 2 O alone, which is a pollution and interference free method. The number of identied proteins increased by 35.05% using Fe 3 O 4 @TMOS, compared to the number of proteins identied when using H 2 O alone as a lysis reagent.   A frequently used lysis buffer, 0.1% SDS, 38 has a robust ability to solubilize and denature membrane proteins. Compared with this reagent, Fe 3 O 4 @TMOS extracted slightly more proteins. However, SDS is hard to remove 39 and interferes with subsequent protein analysis by MS. 40 When the concentration of SDS was increased to 4% followed by FASP purication, a large number of proteins were lost, and the number of identied proteins by MS decreased to 2934. It is interesting to note that Fe 3 O 4 @TMOS can effectively avoid this problem because of its easy removal by a magnet (Fig. 2); thus, there is no impact on MS analysis.
Another widely used lysis buffer is 8 M UA 13,26 which is highly efficient at denaturing and solubilizing proteins by decreasing the hydrophobic effect and directly binding to proteins via hydrogen bonds. [41][42][43] The number of proteins extracted from HeLa cells with Fe 3 O 4 @TMOS and identied by MS was nearly the same as the number extracted using 8 M UA, which is regarded as a good cell lysate reagent. UA has shortcomings, however, including poor thermal stability and a high salt concentration, thus, it cannot be heated, or else protein amidation can occur, and it must be diluted to less than 2 M before enzymatic hydrolysis. In contrast, the samples can be heated to shorten the reaction time when Fe 3 O 4 @TMOS is used, and it can be easily removed to avoid enzyme deactivation in the next processing step.
Three replicated experiments were performed (Fig. S5-S7 †) and a total of 5092 proteins extracted by Fe 3 O 4 @TMOS were identied. In all the above results indicated that Fe 3 O 4 @TMOS is comparable to SDS and UA when used for protein lysis, but unlike these commonly used reagents, Fe 3 O 4 @TMOS can be heated and then be removed by a magnet to shorten the reaction time, simplify the experimental steps and reduce sample loss, no negative impact on mass spectrometry analysis. These suggest that Fe 3 O 4 @TMOS has a strong ability to denature and solubilize proteins and can be used in future proteomics studies.

Conclusions
An immobilized surfactant was prepared by modifying the long carbon chain on the surface of magnetic nanoparticles. The sample solution and the immobilized surfactant can be easily separated by applying an external magnetic eld before digestion or LC-MS/MS analysis procedures. This avoids interference from surfactants, which are oen difficult to be removed in conventional experiments in downstream MS analysis of proteins. The protein denaturation and solubilization abilities of synthesized Fe 3 O 4 @TMOS were veried with BSA and protein extraction of HeLa cells. The experimental results showed that the average amino acid sequence coverage was 42.1% for BSA denatured by immobilized surfactant, which increased by 76.89% compared with that of the undenatured BSA digest. Additionally, Fe 3 O 4 @TMOS achieved the same denaturation level as that of the calefaction method. When using immobilized surfactant to extract and denature HeLa proteins, the number of proteins subsequently identied by MS was comparable with that of 8 M UA and 0.1% SDS. These results demonstrate that immobilized surfactant has a good ability to denature and solubilize proteins, avoids the shortcomings of both UA and SDS, and can be utilized in the pretreatment of biological samples for analysis by MS.

Conflicts of Interest
The authors declared no conict of interest.