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MARKING RED‐SCALE WITH CARBON‐14 AND ITS EFFECT ON REPRODUCTION AND SURVIVAL

The possibility of using 14C to mark red‐scale for ecological studies has been investigated. Citrus leaves were labelled with 14C to have an activity in the range 3–300 μ Ci gm−1. All insects feeding on these leaves became radioactive. Mature females had an activity of 5000–160,000 c.p.m., mature males an activity of 1000–16,000 c.p.m. and the progeny of the females labelled to the level of 10,000 c.p.m. had an activity of 10–30 c.p.m. Higher levels of activity affected the development and fecundity of the insects. High mortality was observed in first‐instar larvae which had an activity ≥10,000 c.p.m. and in second‐instar larvae which had an activity ≥20,000 c.p.m. Fertilized females which had an activity of 30,000 c.p.m. did not reproduce.