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1017 INFLUENCE OF LABOR ON CORD BLOOD LYMPHOCYTE (1ym) POPULATIONS

We have previously demonstrated that cord blood lym proliferative responses(J Clin Immuno/Immunopath, 30, 1984) and levels of pokeweed induced antibody secreting cells (Pediatr Res, in press) are greater among neonates delivered by Cesarean section(CS) than among those delivered vaginally. These increases were noted to be related to the absence of labor prior to CS. To determine if the presence of labor prior to delivery influenced the numbers and/or proportions of cord blood(CB) T-lym populations, these cells were identified in 46 term neonates (17 delivered vaginally, 29 CS). Mononuclear cells were isolated on a ficollhypaque gradient. T-lym were identified with the monoclonal antibodies OKT3(all T-lym), OKT4(helper-inducer lymphocytes) and OKT8 (suppressor/cytotoxic T cells) using flow cytometry after indirect immunoflucrescent labeling.*p values: labor vs no labor <.002, vaginal vs no labor <.0013 These data suggest that labor influences the proportions of CB T-lym subsets without changing the total number of lym. Changes in T-lym subpopulations could explain differences in T and B lym responses in newborns delivered by CS.