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Isolation and characterisation of microsatellite markers for the dwarf sheoak Allocasuarina humilis (Casuarinaceae), an important understorey species in ecological restoration

Eight polymorphic microsatellite markers were developed for Allocasuarina humilis (Otto & A.Dietr.) L.A.S.Johnson, a common woody shrub endemic to south-west Australia that is a common component of the understorey across a range of vegetation types. The markers were isolated using 454 shotgun sequencing and tested on 48 individuals from two natural populations. We found six to 19 alleles per locus (mean 12.1) and observed heterozygosity ranged from 0.208 to 0.956 (mean 0.710). The markers will be used to assess population structure and diversity in A. humilis, with the aim of developing appropriate seed sourcing strategies for ecological restoration.