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Stoichiometrically Minimal Source Pathways via Model Checking

We formulate the problem of finding stoichiometrically minimal source pathways (SMSPs) in biochemical metabolic graphs and present a model checking approach to solve it. SMSPs are paths whose source nodes correspond to native metabolites and which use a non-dominated amount of those compounds. Our approach allows one to eliminate inefficient pathways when selecting the best path to a target. We also investigate the impact of the choice of model checking technique on the runtime for our procedure.