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One-electron reduction of 5-deazalumiflavin in aqueous solution: a pulse radiolysis study.

The one-electron reduction of 5-deazalumiflavin has been studied in aqueous solution in the acidity range H0 = -1 to pH 13 using the reducing species CO2-, e-aq and (CH3)2COH radicals. The spectral and other properties of the deazaflavin radicals formed were found to be independent of the reductant used. Four protolytic forms of the radical were distinguished with associated pKa values of 1.3 +/- 0.3, 6.0 +/- 0.3 and 10.7 +/- 0.3.