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Distinct functions of calmodulin are required for the uptake step of receptor‐mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets

The uptake step of receptor‐mediated endocytosis in yeast is dependent on the calcium binding protein calmodulin (Cmd1p). In order to understand the role that Cmd1p plays, a search was carried out for possible targets among the genes required for the internalization process. Co‐immunoprecipitation, two‐hybrid and overlay assays demonstrated that Cmd1p interacts with Myo5p, a type I unconventional myosin. Analysis of the endocytic phenotype and the Cmd1p–Myo5p interaction in thermosensitive cmd1 mutants indicated that the Cmd1p–Myo5p interaction is required for endocytosis in vivo. However, the Cmd1p–Myo5p interaction requirement was partially overcome by deleting the calmodulin binding sites (IQ motifs) from Myo5p, suggesting that these motifs inhibit Myo5p function. Additionally, genetic and biochemical evidence obtained with a collection of cmd1 mutant alleles strongly suggests that Cmd1p plays an additional role in the internalization step of receptor‐mediated endocytosis in yeast.