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Effect of black-pigmented Bacteroides gingivalis on cytotoxic activity of linolenic acid against mouse macrophages.

The effects of sonicates of black-pigmented Bacteroides gingivalis (Bg-sonicates) on the cytotoxicity of linolenic acid (LA) against mouse macrophages were examined. Treatment of macrophages with LA alone or in combination with Bg-sonicates resulted in the release of lactate dehydrogenase (LDH) from the cells. This observation suggests the cytotoxicity of LA. Also, an increase in thiobarbituric acid-reactive materials was observed in experimental systems of the addition of Bg-sonicates to LA. The cytoplasm of macrophages treated with LA was not stained with May-Grünwald-Giemsa solution, while neither the nucleus nor the cytoplasm was stained when the cells were treated with LA and Bg-sonicates. The above cytotoxicity was nearly abolished when LA and Bg-sonicates were preincubated before the addition to macrophages. B. gingivalis is considered to increase the extent of the cytotoxic effects of LA to the cell nucleus by promoting peroxidation of LA.