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Mode of Action

Inhibits protein synthesis; binds to 30S ribosomal subunit.

The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.

Mechanism of Resistance

Usage Notes

  1. 100 mg/mL stock solution in H2O.
  2. Filter-sterilize with 0.22 μm filter.
  3. Add 1 mL per liter of culture.


