%0 DATA %A María, Soledad Gutiérrez %A María, Cecilia Rojas %A Dionisia, Sepúlveda %A Marcelo, Baeza %A Víctor, Cifuentes %A Jennifer, Alcaíno %D 2015 %T RT-qPCR for CBR.1. CBR.2, CYB5 and crtR transcript levels in the wild-type and mutant strains. %U https://plos.figshare.com/articles/_RT_qPCR_for_CBR_1_CBR_2_CYB5_and_crtR_transcript_levels_in_the_wild_type_and_mutant_strains_/1574653 %R 10.1371/journal.pone.0140424.g006 %2 https://plos.figshare.com/ndownloader/files/2358757 %K Cytochrome b 5 Reductase %K cpr %K crtR %K cytochrome P 450 systems %K carotenogenic yeast Xanthophyllomyces dendrorhous %K ergosterol biosynthesis %K CYB 5 %K cytochrome P 450 reductase %K cytochrome P 450 enzyme %K cytochrome P 450 redox partner %K alternative electron donor pathway %K dendrorhous CBR encoding gene %K CBR .1 %K CYP 61 genes %K nadph %K alternative cytochrome P 450 electron donors %K cytochrome b 5 %K strain %K alternative electron donors %K P 450 reactions %K P 450 enzymes %X

(A) CBR.1 (B) CBR.2, (C) CYB5 and (D) crtR genes. The transcript level of each gene was normalized to actin mRNA. Black and grey bars represent the results using RNA extracted after 36 and 72 h of culture, respectively. Values are the mean ± standard deviation of two technical replicates from three independent cultures (Student’s t-test was performed to compare each mutant to the parental strain. * P <0.05 and ** P <0.01).