Ming Jiang
Last active: 7/6/2016

[WAF1/CIP1/p21 gene in wild type p53 and mutant p53 human breast cancer cell lines in relation to its cytobiological features].

Jiang M, Shao Z, Wu J
Zhonghua Zhong Liu Za Zhi. 1998 20 (3): 181-4

PMID: 10921001

OBJECTIVE - To study the WAF1/CIP1 gene DNA status, mRNA and protein expressions in human breast cancer cell line and its significance.

METHODS - Using cell culture, molecular biological techniques such as Southern blot, Northern blot and immunocytochemical methods, the WAF1/CIP1 gene DNA status, mRNA and protein expression levels in MCF-7 expressing wild type p53(wtp53) and MDA-MB-231 expressing mutant p53 (mtp53) human breast cancer cell lines were detected respectively. The p53 and mdm-2 protein expression levels and cytobiological features of the 2 cell lines were compared and correlated to their WAF1/CIP1 gene expression levels.

RESULTS - (1) There was no difference in WAF1/CIP1 gene DNA status in the two breast cancer cell lines. Neither of them showed gene amplificatian or deletion. However, the WAF1/CIP1 mRNA and p21WAF1/CIP1 protein expression levels of MCF-7 cells were higer than those of MDA-MB-231 cells (P < 0.05). (2) The character and cellular distribution of p53 protein in the two cell lines were clearly different. The expression level of mdm-2 proteion was significantly higher in MCE-7 than in MDA-MB-231 cells (P < 0.05). (3) Compered to the other breast cancer cell line, MCF-7 cells were better differentiated, grew more slowly and adhered more closely with each other.

CONCLUSION - The WAF1/CIP1 gene expression at mRNA and protein levels is associated with p53 phenotype and some cytobiological features of human breast cancer.

MeSH Terms (13)

Breast Neoplasms Cyclin-Dependent Kinase Inhibitor p21 Cyclins Female Genes, p53 Humans Mutation Neoplasm Proteins Nuclear Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-mdm2 RNA, Messenger Tumor Cells, Cultured

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