kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human FcGR2A / CD32a (H167) Proteing, 100µg  

Recombinant Human FcGR2A / CD32a (H167) Proteing, 100µg

Recombinant Human FcGR2A / CD32a (H167) Protein, Ala 36 - Ile 218, produced in human 293 cells, His-Tag,  SPR & BLI verified

Recombinant, human, protein CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

More details


Availability: within 7 days

336,00 €

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

Recombinant Human CD32a (H167), His Tag (SPR & BLI verified) (CD1-H5223) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1).
Predicted N-terminus: Ala 36

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.5 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg of the Human FcGR2A / CD32a (H167), His Tag by the LAL method.

>95% as determined by SDS-PAGE.




Please refer to product data sheet.


Use BIAFFINS FcyR BINDING ASSAYS for detailed kinetic analysis of your antibodies using SPR.

Figure: Real-time kinetic analysis of antibody binding to FcγRIIa using surface plasmon resonance.


(1) Ravetch JV. et al., 2001, Annu Rev Immunol. 19: 275-83.
(2) Takai T. 2002, Nature Rev Immunol. 2: 580-92.
(3) Nagarajan S. et al., 2000, Blood. 3: 1069-77.(3) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.

The following products could also be interesting for you: