Reveal® Q+ for Aflatoxin

Item No.  8085

  • For use with portable reader with data management software
  • Rapid and accurate quantitative testing
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Minimum quantity required: 1
Reveal® Q+ for Aflatoxin is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format intended for the quantitative testing of aflatoxin in grain and grain products.
Analyte Aflatoxin
Approvals FGIS 2018-114; FGIS02019-119
Brand Reveal®
Limit of Detection 2 ppb
Package Dimensions 8.40 IN x 5.20 IN x 2.40 IN
Package Weight 0.33 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 3.00 ppb - 100.00 ppb
Result Type Quantitative
Testing Time 6.00 Minutes

  1. 25 Reveal Q+ for Aflatoxin test strips
  2. 25 red sample dilution cups
  3. 25 clear sample cups
  4. 1 bottle of sample diluent
  5. Instructions for use
  1. 65% ethanol solution (NEOGEN® item 8073, 8074)
  2. Sample collection cups with lids (NEOGEN item 9428)
  3. Agri-Grind grinder or equivalent (NEOGEN item 9401, 9453)
  4. Scale capable of weighing 5–50 g ± 0.1 g (NEOGEN item 9427)
  5. Timer (NEOGEN item 9426, 9452)
  6. Reveal sample cup rack (NEOGEN item 9475)
  7. Raptor Integrated Analysis Platform (NEOGEN item 9680), AccuScan Gold or AccuScan Pro
  8. Raptor cartridges (NEOGEN item 9681)
  9. Dispensing pump or graduated cylinder (NEOGEN item 9448, 9447)
  10. Filter syringe (NEOGEN item 9420)
  11. Sample collection tubes with caps (NEOGEN item 9421, 9421B)
  12. Pipettor, 100 µL (NEOGEN item 9272, 9860)
  13. Pipette tips, 100 µL (NEOGEN item 9407, 9410, 9417)
  14. Pipettor, 500 µL (NEOGEN item 9336, 9291)
  15. Pipette tips, 200–1000 µL (NEOGEN item 9464, 9487, 9293, 9292)


Assay Principle

Reveal Q+ is a single step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for toxin conjugated to colloidal gold particles. If the toxin is present, it will be captured by the particle-antibody complex. The toxin-antibody-particle complex then is wicked onto a membrane, which contains a zone of toxin conjugated to a protein carrier. This zone captures any uncomplexed toxin antibody, allowing the particles to concentrate and form a visible line. As the level of toxin in a sample increases, free toxin will complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of toxin in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® readers convert these line densities into a quantitative result displayed in ppb or ppm. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of toxin, ensuring the strip is functioning properly.





