Negative Stain Single Particle Analysis

There are some special problems which can arise when processing negative stain data. EMAN2 was primarily designed to work with Cryo data, so you may need to do things a little differently to get good results with negative stain.

Normally people who know negative stain well, and have nicely stained images will collect their images at or near Scherzer defocus (ie - very close to focus). This minimizes visible CTF artifacts in the image, and since most of the contrast provided by negative stain is amplitude contrast, there is no need to defocus. Other people choose (for various reasons) to image far from focus as if they were working in Cryo.

If working close to focus (<0.5 microns)

If working far from focus (anything >0.5 microns)

EMAN2/NegativeStain (last edited 2020-10-09 12:29:59 by SteveLudtke)