MACSPlex Assays are designed for determining concentrations of multiple soluble analytes in a single sample. The analysis is based on MACSPlex Capture Beads, which display defined fluorescence properties and can be identified using standard flow cytometry techniques. MACSPlex Kits contain a cocktail of various MACSPlex Capture Beads populations, each coated with a specific antibody reacting with one of the respective analytes within the sample. Analytes are labeled directly or indirectly (via biotin) with APC-conjugated antibodies, depending on the chosen Kit. Standards of known quantities of given analytes are provided with the kit and are used for the quantification of the unknown analyte concentrations within the samples.