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Spectrofluorimetric Determination of Human Serum Albumin Using a Tetracycline–Europium Complex

Abstract A new spectrofluorimetric method is proposed for determination of human serum albumin (HSA). Using tetracycline (TC)–europium (Eu3+) as a fluorescent complex, in the buffer solution of pH = 10.2, HSA can remarkably enhance the fluorescence intensity of the TC–Eu3+ complex at λ = 612 nm and the enhanced fluorescence intensity of Eu3+ ion is in proportion to the concentration of HSA. Optimum conditions for the determination of HSA were also investigated. The linear ranges for HSA are 0–2.5 × 10−5 mol L−1 and 2.5 × 10−5–2.0 × 10−4 mol L−1 with limits of detection are 2.4 × 10−6 mol L−1 and 1.5 × 10−5 mol L−1, respectively. This method is simple, practical, and relatively free interference from coexisting substances and can be successfully applied to determination of HSA in samples of serum and urine.