{'fraction_non_alphanumeric': 0.03944672131147541, 'fraction_numerical': 0.022540983606557378, 'mean_word_length': 5.279742765273312, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Mapping of linear antigenic determinants on glycoprotein C of herpes simplex virus type 1 and type 2 recognized by human serum immunoglobulin G antibodies

Using membrane‐based dekapeptides, the reactivity of human serum antibodies with linear antigenic determinants of herpes simplex virus (HSV) type 1 and type 2 glycoprotein C (gC‐1, gC‐2) was studied by pep scan and immunodot assay. The entire coding sequences of gC‐1 and gC‐2 were screened for the presence of linear epitopes by pep scan. Peptides recognized in an HSV‐1 type‐specific manner were mainly identified within the N‐terminal third and at the C‐terminus of gC‐1, whereas most type‐common antibodies were directed against colinear peptides within the central parts of gC‐1 and gC‐2. The type‐specific reaction of human sera with gC‐2 peptides in pep scan was poor. Eight peptides identified as immunoreactive by pep scan were further tested in immunodot assay for their reactivity with a human serum panel. None of the eight HSV‐negative sera gave positive results by immunodot assay. Positive reactions with gC peptides were found to be strongly age‐dependent, i.e., the rate of positive reactions was significantly higher in HSV‐positive adults than in HSV‐positive children. Antibody reactivity with two type‐common gC peptides was demonstrated in 17 out of 28 HSV‐positive sera. A putative type‐specific gC‐2 peptide employed in immunodot assay was inconsistently recognized by human sera. Twenty HSV‐positive sera reacted with at least 1 of 5 type‐specific gC‐1 peptides. Nine sera showing no reactivity with glycoprotein G of HSV‐1 (gG‐1) by immunoblotting recognized type‐specific gC‐1 peptides in immunodot assay. Thus, gC‐1 peptides might allow the detection of HSV‐1‐specific antibodies in individuals showing no reactivity with commonly employed HSV‐1‐specific diagnostic antigens, i.e., purified or recombinant gG‐1. J. Med. Virol. 55:281–287, 1998. © 1998 Wiley‐Liss, Inc.