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Use of Raman spectroscopy to study the interaction of antitumor drugs and DNA

The binding of two isomeric 2-[(chysenylmethyl)amino]-2-methyl- 1 ,3-propanediols (AMAPs) to the biopolymer poly(dG-dC)•poly(dG-dC) was studied using Raman spectroscopy. The Raman spectra of the bound AMAPs were obtained by subtracting the Raman spectrum of the biopolymer in buffered aqueous solution from those recorded for the AMAP-DNA binary complexes. The difference method applied here has been used in other studies to obtain spectra of small molecules bound to macromolecules of biological origin (Yue, K.T. et al., J. Raman Spectrosc., 20, 541-545, 1989, and references therein). Due to the extremely low number of Raman active lines observed in the spectra of these AMAPs, it was also possible to study perturbations in the Raman spectrum of the complexed biopolymer. Spectroscopic data indicated that both AMAPs intercalate with DNA, in agreement with viscometric measurements for these compounds; however, some differences were observed in the Raman spectra of DNA complexed with these drugs, suggesting that the specific AMAP-DNA binding interactions are different.