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A variety of Staphylococcal plasmids present as multiple copies.

In enteric bacteria estimation of the number of plasmids per chromosome reveals two distinct situations (Clowes, 1972): first, when the number of copies is approximately one; and second, when the number of copies is much greater (generally between 7 and 40). This division may also exist for staphylococcal plasmids (Novick & Bouanchaud, 1971), but to test this possibility more fully we have determined the number of plasmid copies per organism for a range of staphylococcal plasmids. We have found that plasmids detectable as covalently closed circular (CCC) DNA are present in multiple copies, i.e. as at least four copies per organism.