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Culture of dermatophytes upon stratum corneum.

An in vitro method of culturing dermatophytic spores on stratum corneum alone is described. The stratum corneum is stripped onto a translucent adhesive tape and fixed to a microscope slide. Spores are applied and the tape is incubated under controlled conditions. The technique has been made semiquantitative and applied to the study of a topical anti- fungal agent.