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Nuclear Magnetic Resonance of Water in Deuterated Citrus Leaf Tissue1


Nuclear magnetic resonance (NMR) H resonance signals of water in deuterated disks from acclimated and nonacclimated satsuma (Citrus unshiu Marcovitch) leaves appear to be a measure of bound water. The D resonance signal was lost when initial freezing occurred along with a partial loss in H resonance, which was attributed to D2O and bound H2O forming ice. Acclimated tissues retained a larger percentage of unfrozen H2O at initial freezing and at several subfreezing temperatures, suggesting that changes in H2O binding occur during cold acclimation. Further, NMR line widths of nonacclimated leaf tissue increased abruptly during freezing and only gradually in acclimated leaf tissue, suggesting increased binding structure of H2O molecules of the bound fraction and cellular constituents in acclimated tissue. Portion of total H2O replaced with D2O was 69% in both, suggesting this portion was free water.