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Microbiological Study in the case of Chronic Inflammatory Bowel Disease

The present study focused on the isolation and identification of the intestinal bacterial ecosystem involved in Crohn’s disease and Hemorrhagic ulcerative colitis. The patients were male and female with an age range of 18 to 60 years. The isolation carried out after grinding the different biopsies collected by means of low endoscopy, on specific selective culture media, made it possible to select a set of bacteria belonging to the intestinal ecosystem. The data were identified phenotypically through the use of biochemical tests, the various API galleries. On the other hand, the anatomopathological study made it possible to pose and confirm the different diagnoses of the pathologies corresponding to our research study. During Gram staining microscopic observation, in the case of Crohn’s disease and Hemorrhagic ulcerative colitis, the identification is mainly represented by the Gram-positive bacilli identified as Clostridium difficile and Bacillus subtilus. These bacterial strains secrete toxins capable of causing an inflammatory state in the intestinal mucosa and thus inducing their pathogenic powers which can cause an immune disorder in these patients. Nevertheless, the Bacillus subtilus strain presents itself as an agent which can induce a defense against the aggressiveness of pathogenic bacteria.