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Permeation of 6-nitro-3-phenylacetamide benzoic acid (NIPAB) and hydrolysis by penicillin acylase immobilized in emulsion liquid membranes.

The effects of various commercial and model surfactants of different structure and hydrophilicity were studied on water-in-oil (w/o) emulsion stability, potassium cation leakage and permeation of 6-nitro-3-phenylacetamide benzoic acid in a model system using Penicillin acylase (EC 3.5.1.11) immobilized in a liquid membrane. Both emulsion stability, potassium leakage and permeation of organic substances depend upon hydrophilicity of surfactants. Hydrophilic surfactants may be used to stabilize emulsions only in mixtures with hydrophobic emulsifiers. Additions of small quantities of hydrophilic surfactants to the system in which permeation occurs together within an enzymatic process may be advantageous. Both the rate of permeation and potassium transfer significantly increase when hydrophilic surfactants are present. There was no relationship observed between potassium cation transfer from the internal phase and emulsion stability in the storage test.