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Molecular characterization of human Sapovirus in untreated sewage in Italy by amplicon‐based Sanger and next‐generation sequencing

Human Sapoviruses (HSaVs) are etiological agents of sporadic cases and outbreaks of acute gastroenteritis in humans of all ages. Evidence of worldwide distribution of HSaV has been documented; however, little is known about HSaV circulation in Italy. To study their occurrence and genetic diversity a nation‐wide environmental surveillance was undertaken.