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Comparison of Murine Sarcoma Viruses in Nonproducer and S+L−-Transformed Cells

The biologic properties of murine sarcoma viruses in nonproducer and S+L− cells were compared. Each virus was found to be genetically stable through at least two cycles of rescue and transmission to new cells. There was no evidence for production of a defective murine leukemia virus by S+L− cells. The results are most consistent with the hypothesis that the sarcoma genome in S+L− cells contains information for replication functions which the virus in nonproducer cells either lacks or does not express.