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Experimental Study on Anti-HSV-2 Effect of CII-3 Extracted from Periplaneta Americana in Vitro and in Vivo

Objective: To study the anti-HSV- 2 effect of CII- 3 extracted from Periplaneta Americana. Methods: Anti-HSV- 2activity of CII-3 extracted from Periplaneta Americana was observed and determined with MTT assay and cytopathic effect(CPE) assayand the value of TC50, IC50 and TI in the extract was calculated. Female mice with genital herpes were used as animal models. 50 μL ofHSV- 2 was injected into mice vaginas once a day with 7 consecutive days. With acyclovir as the positive control, intragastricadministration was adopted as treatment for 10 days. The ELISA KIT was used to test the concentration of vascular tumor necrosisfactor-α(TNF-α), Interferon-γ(IFN-γ) and Interleukin-2(IL-2); the immune organs were weighed to calculate the organ index.Results: CII-3 extracted from Periplaneta Americana had anti-HSV-2 activity. The TC50 value was 190.55 μg/m L, IC50 value was28.71 μg/m L and TI value was 6.64 μg/m L. CII-3 had the function of increasing TNF-α, IFN-γ, IL-2 and could increase the weightof the immune organs in mice. Conclusion: CII-3 extracted from Periplaneta Americana had the effect of anti-HSV-2 by boosting thebody's immunity.