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Estimation of Pathways of Glucose Metabolism Employing [14C] Glucose

A method of quantitative estimation of the contribution of the pentose cycle and the Embden-Meyerhof pathway to erythrocyte carbohydrate metabolism employing [1-14C] glucose and [6-14C] glucose, is described. It has been found that the ratio of both pathways changes with time of incubation. The contribution of the pentose cycle to glucose metabolism was about 50% and below 10% after 1 hour and 3-5 hour incubation, respectively. Methylene blue (1.84 × 10-4 м) increased the contribution of the pentose cycle to glucose metabolism from 1.5 times to 7 times after 20 min und 3 hour incubation, respectively. In view of the inhibitory effect of ATP on glucose-6-phosphate dehydrogenase (ᴅ-glucose- 6-phosphate: NADP+ oxidoreductase EC 1.1.1.49) the dependence of the contribution of the pentose cycle to glucose metabolism on the length of incubation may be related to the possible changes of erythrocyte ATP content during the separation and washing of these cells.