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Genetic recombination in Streptomyces michiganensis DSM 40 015 revealed three genes responsible for the formation of melanin

By UV and NTG mutagenesis numerous auxotrophic, antibiotic‐resistant and melanin‐negative mutants were isolated from Streptomyces michiganensis DSM 40 015 which proved to possess a efficient photoreactivation system. Using a mel− test strain with three auxotrophic markers and a antibiotic resistance for crosses with numerous prototrophic mel− strains three classes of mutants (melA, melB and melC) could be found. This classification was further supported by a series of crosses. The melC locus seemed to correspond to the melC locus of S. glaucescens which contains the tyrosinase structural gene.