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Generation of coagulation factors V, XI, and XII by the isolated rat liver.

When isolated rat livers were perfused with platelet-free erythrocytes suspended in Tyrode's solution containing 6% bovine serum albumin, the generation of factors V, XI, and XII was clearly demonstrable. On average, after 5 h of perfusion of a single liver, the concentrations in the perfusate (as a percentage of normal rat plasma) were about 6% for factor V, 8% for factor XI, and 5% for factor XII, compared with 20% for factor VII, which was used as the reference standard. When two livers were perfused, approximately twice these concentrations were achieved. When the properties of these factors in perfusate and plasma were compared, they agreed well except for differences in the celite adsorbability of factors XI and XII.