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Validation of Vacuum Consistency of The Internal Evacuated Blood Collection Tube (BCT)

Evacuated blood collection tube (BCT) or also known as Vacutainer® is a device which accumulate negative pressure as a function of vacuum to collect blood as a specimen for pharmaceutical analysis. Nevertheless, BCT consist of three (3) components which is transparent tube, rubber stopper, and hemogard closure. Study of vacuum on BCT has become interest in many researchers about its consistency due to effect by its surrounding condition. In this research, impact of storage temperature will be studied to identify the effect of vacuum content within BCT, also to investigate the problem and seek proper (surrounding) placement for these BCT. At the same time, it should develop with a new benchmark vacuum volume (negative pressure) inside BCT aiming to obtain the standardise figure. This BCT should be placed under temperature condition between 4°C to 25°C as suggested by BD-Vacutainer® company and under certified humidity condition are ranged between 40% to 60%. It is to ensure the pharmaceutical device which is sterile and consist of shelf life does not deplete its properties drastically and sustain its life span as recommended based on technical data sheet. Thus, the amount of negative pressure in BCT presentively indicate as a shelf life.