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DNA Synthesis of Wing Disc Cells and the Effects of Mitomycin C and X Ray Irradiation on the Wing Development of Bombyx mori

Abstract The rate of DNA synthesis of wing discs and the effects of X ray irradiation and mitomycin C injection were observed during last larval and early pupal stage of Bombyx mori. The rate of DNA synthesis in wing discs of Bombyx mori was estimated by means of the incorporation of 3H-thymidine during the fifth larval stage. Mitomycin C injection and X ray irradiation were performed during the fifth larval instar and pupal stage to investigate the effects of inhibition of DNA synthesis. The rates of 3H-thymidine incorporation were high on days 0, 1, 2, 4, 5, 7, 8 and 10 of the fifth larval instar. Wings of eclosed adults were affected by injections of mitomycin C between the 7th day of the fifth larval instar and the second day of the pupal stage. Exposing the insects to X rays during the fifth larval instar affected wing and scale formation, and at earlier stages, it had a more deleterious effect. Irradiation at 24–36 hours after pupation inhibited wing expansion and scale formation most among pupal stages. The results suggested that the peaks of 3H-thymidine incorporation during the fifth larval instar and the pupal stage have specific significance for wing development.