{'fraction_non_alphanumeric': 0.02962962962962963, 'fraction_numerical': 0.008148148148148147, 'mean_word_length': 5.788944723618091, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
THE EFFECT OF PARAOXONASE 1 ON ENDOTHELIAL CELL HYPERPOLARIZATION MEDIATED BY EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID DERIVATIVES

Objective: It has been claimed that Paraoxonase 1 (PON1), an antiatherogenic high-density lipoprotein (HDL)-associated enzyme, protects against cardiovascular disease (CVD), for which hypertension is a major risk factor. On the other hand, an increasing number of studies associate heightened PON1 activity with increased risk of hypertension and CVD. Our previous findings revealed that 5,6-EET, a cytochrome P450 (CYP)-derived arachidonic acid (AA) metabolite is unstable, transforming into a new derivative, which is a potential endothelium-derived hyperpolarizing factor (EDHF). This derivative mediates calcium influx, leading to endothelial hyperpolarization and vasodilation and has also been shown to be hydrolyzed by PON1. Based on the similarity between the chemical structure of CYP-derived metabolites of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to those of AA, we hypothesized that the analogous EPA and DHA derivatives could potentially induce endothelial hyperpolarization and elicit vascular dilation. The aim of this study is to explore hyperpolarization of endothelial cells induced by derivatives of EPA and DHA and PON1’s role in hypertension via its effect on this process.