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Isolation and Identification of Clostridium butyricum CD-8 from Chinese Traditional Medicine Wastewater and Study on Its Acetic Producing Conditions

Objective: In order to improve the efficiency of Chinese traditional medicine wastewater by anaerobic digestion,high efficient anaerobic hydrolysis of acid producing bacteria were isolated and identified, moreover its acid production characteristics were studied. Method: Strains were isolated by hungate roll- tube technique from Chinese traditional medicine wastewater in Shijiazhuang and identified by physiological and biochemical characteristics,morphology,as well as sequence analysis of 16S rDNA. Result: The results indicated that the strain CD- 8 belonging to Clostridium butyricum. It was provided with an optimal ability of growth and metabolism at 37℃ and with an initial pH 8. 0. The acetic acid production was promoted in the condition of 0 Na+and stimulated with addition of 8 mmol / L K+. Sucrose,solvable starch and maltose were optimal carbon sources for cell growth and acetic production. Conclusion: The strain CD-8 grew rapidly,which has a wide range of substrate utilization and a highly efficiency of acid production. At the same time,CD- 8 possessed the potential application in improving the efficiency of hydrolysis acidification and herb residues of biogas utilization.