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Calcium fluoride formation on sound enamel using fluoride solutions with and without lactate.

The formation of calcium (Ca) fluoride (CaF2) on bovine enamel blocks during clinically relevant treatment times using neutral fluoride (F) solutions (0.26 mol/l F) with and without 0.1 mol/l lactate was investigated. Uncoated and pellicle-coated blocks were evaluated for alkali-soluble (1 mol/l KOH, three consecutive 24-hour treatments) Ca, PO4, and F after treatment by the F solutions for 0, 5, 15, 30, and 60 min. There was an overall time-related increase in F recovery, while Ca tended to remain at baseline levels. Less F was recovered from the pellicle-coated blocks. The addition of lactate to the F treatment solution did not result in an overall increase in alkali-soluble F recovery, but did result in the formation of cuboidal shaped crystals which closely approached the morphology of pure CaF2. A 1:2 stoichiometric ratio Ca:2F (mol:mol) was not established based on chemical analyses. The ultrastructural and elemental composition of surface deposits on the samples, as determined using scanning electron microscopy, X-ray diffraction, and energy-dispersive spectroscopy, established the presence of CaF2 after 24-hour F treatments; however, it was not possible to directly demonstrate the formation of CaF2 after clinically relevant treatment times.