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Use of RP-TLC and Densitometry to Analytical Characteristic of Vitamin K1

Abstract Vitamin K1 (phylloquinone) was investigated with the use of reversed phase thin layer chromatography (RP-TLC) on RP8F254s, and RP18F254s (E. Merck) plates, using methanol as a mobile phase. It was stated that a larger amount of the bands on a chromatogram were obtained on RP8F254s plates than on RP18F254s plates. Six densitometric bands on RP8F254s plates were observed, where vitamin K1 had RF = 0.45 and λmax = 254 nm; however, the impurities had the RF values equal to 0.27, 0.54, 0.59, 0.62, and 0.75, respectively. It was stated that RP-TLC with densitometry and spectrodensitometry is a good method for the analysis of the qualitative composition of vitamin K1.