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In vitro Culture of Echinacea purpurea L.

ABSTRACT In vitro tissue culture protocols were tested for the propagation of Echinacea purpurea L. (purple coneflower) using Murashige and Skoog Minimal Organics (MSMO) with combinations of naphthalene acetic acid (NAA) and kinetin and 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin. Hypocotyl explants formed more shoots and roots at NAA-kinetin levels of 1:1 and 2:1 (NAA:kinetin) mg/liter of media than any other hormone regime tested. Extensive callus and a limited number of shoots and roots formed on media with 2,4-D-kinetin combinations. Explants on media with NAA-kinetin produced mature plants with flowers true-to-type in color and form.