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Sedimentation analysis of deoxyribonucleic acid from thymine-starved Escherichia coli

During thymine starvation, strand breaks accumulate in the chromosomal deoxyribonucleic acid (DNA) of Escherichia coli. This effect occurs to a varying extent in different strains and is particularly enhanced in strains deficient in DNA polymerase I. The inhibition of ribonucleic acid or protein synthesis suppresses the accumulation of strand breaks. In a polA strain, rifampin is more effective than chloramphenicol or puromycin in suppressing strand break accumulation. To a certain extent the pehenomenon othymineless death correlates with the appearance of strand breaks. Although the killing can not be explained by the bulk of strand breaks, it is possible that some of them represent lethal events. On the basis of our observations we proposed the following model. (i) Transcription may be accompanied by single-strand breaks in DNA. (ii) DNA polymerase I is involved in the efficient repair of these breaks. (iii) Thymine deprivation results in the accumulation of unrepaired breaks. (iv) Polymerase I-mediated repair is less affected by thymine deprivation than are the alternative pathways because it closes the breaks with short patches, requiring less thymine.