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A New Method for Analyzing the Duffy Blood Group Genotype by TaqMan Minor Groove Binding Probes

Duffy blood group genotyping is useful to ensure transfusion safety and determine the association of Duffy blood group polymorphism with diseases, and therefore has its clinical significance. In order to improve the existing methods for genotyping of Duffy blood group, which normally require post‐PCR manipulation, a new method was developed by using 5′‐nuclease assay (NA) with TaqMan minor groove binding (MGB) probes.