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Evaluation of Antibacterial Potential of CS/Fe3O4 Nanocomposites Based Plate Count Method

In this study, magnetite nanoparticles (Fe3O4 NPs) and Chitosan coated Fe3O4 nanocomposites (CS-Fe3O4 NCs) were synthesized by simple co-precipitation method under air atmosphere. The structure, Functional, morphology
 and magnetic property of synthesized samples have been analyzed. X-ray diffraction results confirmed NPs and CS-Fe3O4 Fe3O4 NCs were composed of pure magnetite and the peak positions were unchanged after the introduction of CS biopolymer. The FTIR
 studies authenticate the bonding of the CS polymer with Fe3O4 NPs. The morphological studies revealed that the nanoparticles are almost spherical in size. Magnetization curves showed both Fe3O4 NPs and CS-Fe3O4 NCs were near
 to superparamagnetic. In addition, the antimicrobial efficacy of CS-Fe3O4 NCs were found out by using viable cell-counting method against Gram positive bacterial strain Staphylococcus aureus MTCC 3160 (S. aureus). The results proved that synergistic antibacterial
 effects by combining CS with Fe3O4 NPs which may have the potential to be used in combating bacterial infections.