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Effect of Denervation on a Cholinergic‐Specific Ganglioside Antigen (Chol‐1) Present in Torpedo Electromotor Presynaptic Plasma Membranes

Abstract: The presence of Chol‐1, an antigen identified in the plasma membrane of cholinergic electromotor nerve terminals of Torpedo marmorata, was investigated in Torpedo electric organ after 3, 6, and 9 weeks’denervation. Denervation was monitored by the cessation of stimulus‐evoked discharge potentials, by the reduction in nerve terminals seen morphologically, and by the decrease in ACh and ChAT contents. The content of ganglioside‐bound sialic acid did not show any appreciable change with time. Some modification of ganglioside pattern on TLC was observed after 9 weeks’denervation. The presence of Chol‐1 after denervation was assayed by its activity in inhibiting the selective complement‐induced lysis of the cholinergic subpopulation of guinea pig cortical synaptosome which is mediated by the anti‐Chol‐1 antiserum. Denervation did not affect Chol‐1 immunoreactivity although it did alter the distribution of the immunoreactivity among gangliosides. The possible significance of the results is discussed.