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Photoluminescence ZnO nanorod biosensors for medical and food safety applications

Photoluminescence (PL) ZnO nanorods (NR) are prospect materials for biosensor applications. They can be deposited on solid state substrates and/or dispersed in liquid media. ZnO NRs demonstrate strong UV emission (3.28–3.3 eV), which depends on surface charge, formed by adsorbed molecules. In the present work we report on ZnO NRs, deposited on glass slides, used as biosensor templates for detection food pathogens. Change of ZnO PL after interaction with biomolecules was recorded. Dependence of PL intensity vs concentration of biomolecules was obtained. In the second part, ZnO nanorods were used as PL biomarkers. A suspension of ZnO NRs, conjugated with SSEA-4 antibodies (aSSEA-4) in PBS buffer was prepared. The suspension was deposited on glass slides with cancer cells. After incubation for 20 minutes and washing, the slides were studied using PL spectroscopy. Due to specific interaction between aSSEA and cancer cells, different PL intensities of ZnO emission were recorded for positive and negative cancer probes. Portable optical bioanalytic system was proposed.