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Automated screening platform with isochronal-parallel analysis and conditioning for rapid method development of chiral separations.

An automated chiral separation-screening platform was developed, allowing separation analysis on one column while another column is simultaneously equilibrated with a different mobile phase (MP). The platform can be set up from usual HPLC components at moderate cost and it considerably speeds up the screening process, allowing numerous chromatographic conditions to be tested within a short period of time and leading to a significant gain in time and productivity in preparative separations.