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[The effect of immune bovine whey on cell-associated glucosyltransferase activity of Streptococcus mutans].

OBJECTIVE
To study the effect of immune bovine whey on cell-associated glucosyltransferase (GTF) activity of S. mutans MT8148.


METHODS
The immune milk was collected from cows immunized with cell-associated GTF overexpression strain B-29-33 of S. mutans MT8148. The control milk was from non-immunized cows. The immune absorbed whey was gotten from immune bovine whey which was absorbed with lyophilized Formalin-killed B-29 whole cells. Three kinds of whey were subdivided into three groups: 50 microliters, 70 microliters, 90 microliters. The content of insoluble glucan was estimated colorimetrically by anthrone method.


RESULTS
The control bovine whey had an enhancing GTF activity (407.00%-485.62%). The immune absorbed whey inhibited partly the enhancing GTF activity (208.74%-273.00%). The immune whey inhibited significantly the GTF activity (70.24%-38.62%) and the inhibition showed a tendency to depend on doses.


CONCLUSIONS
The immune bovine whey inhibits significantly the cell-associated GTF activity of S. mutans MT8148.