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Detection of ethylene for in vitro evaluation of the uv photoprotection

In this study, the antioxidant capacity during UV exposure has been investigated by using a laser-based trace gas detector for the detection of gaseous ethylene as an indicator for lipid peroxidation. Biocream was used as a control and ascorbic acid, butylated hydroxytoluene, phloroglucinol and hydroquinone were added as antioxidants in a mixing concentration of 2.5% (w/w). The experimental results show that there is a decrease in the number of ROS due to the presence of antioxidants, from which ascorbic acid is the most effective. The detection method offers a direct and on-line way to evaluate the capability of antioxidant in reducing the harmful effect of the UV radiation. INTRODUCTION: In the past various in vitro and in vivo methods have been presented to evaluate the photo protection efficacy of sunscreen based on the measurement of sun protection factor (SPF), UVA/UVB ratio, and their critical wavelength 1, 2, 3, 4, 5 . The determination of SPF in vivo is time consuming and expensive; in addition, the method is more suitable for testing the effect of UVB radiation due to its exclusion of UVA radiation 4, 5 .