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[Determination of ilexside II in leaves of Ilex cornuta by HPLC-ELSD].

OBJECTIVE
To develop a HPLC-ELSD method for determination of ilexside II in leaves of Ilex cornuta.


METHOD
The separation was performed on a Waters Syemmetry Shield RP18 column (4.6 mm x 250 mm, 5 microm) and methanol-water was used as mobile phase in gradient elution. The flow was 1.0 mL x min(-1), and the temperature was set at 35 degrees C.


RESULT
The calibration curve showed good linearity in the test range (R2 = 0.9997). And the mean recovery was 101.3%, RSD was 2.0% (n=6).


CONCLUSION
The developed HPLC-ELSD method was accurate and reproducible, and can be used for the determination of ilexside II in leaves of Ilex cornuta.