{'fraction_non_alphanumeric': 0.02512998266897747, 'fraction_numerical': 0.025996533795493933, 'mean_word_length': 5.715116279069767, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Effects of Curcumin on the Cytoskeleton of OSCC Cells by Rho/ROCK Pathway

Objective: The present article aimed to study whether the effects of curcumin are related to cytoskeleton variation in OSCC cell line HN13 and its mechanism.Methods: HN13 cells were treated and exposed with different concentrations of curcumin(0,10,20,40 μmol/L).After 24 h,Coomassie blue staining was used to measure the cystoskeleton o f HN13 cells.Immunohistochemistry was used to observe the expression of F-actin.The expression of ROCK2 and phosphated ROCK2 were detected by Western blot.Results: Qualitative morphological evaluation revealed that HN13 cell exposed to curcumin with different concentration became obvious deformation.The cytoskeletal structures shrinked,the staining became faded,stress fibers reduced.Immunohistochemistry revealed the staining of F-actin appeared somewhat weaker in HN13 cells which exposed in high concentration of curcumin.When treated for 24 h,the expression of ROCK2 and p-ROCK2 in HN13 cells was decreased.Conclusion: Curcumin may trigger alterations of the cystoskeleton of HN13 cell line by inhibiting activity of Rho/ROCK2 pathway.