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Pressurized Planar Electrochromatographic Separation of the Enantiomers of Tryptophan and Valine

Pressurized planar electrochromatography (PPEC) with commercially available chiral plates has been used for separation of the enantiomers of tryptophan and valine. The effects on migration distance of polarization voltage, buffer pH and concentration, and the concentration of the organic component of the mobile phase have been investigated. Solute separation is compared for PPEC and conventional planar chromatography.