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Comparison of Corynebacterium Parvum With Freund's Complete Adjuvant as a Potentiator of the Immune Response To β‐Human Chorionic Gonadotropin Linked to Tetanus Toxoid

ABSTRACT: Corynebacterium parvum was compared with Freund's complete adjuvant (FCA) for potentiation of the rabbit immune response to β‐human chorionic gonadotropin linked to tetanus toxoid (β‐hCG‐TT). With each adjuvant, antibodies to hCG were detected using passive hemagglutination. Higher antibody titers were produced by FCA‐treated animals. The ability of antisera to β‐hCG‐TT to neutralize the biological action of native hCG was determined by the rat uterine weight assay. Anti‐β‐hCG‐TT sera from C. parvum‐treated rabbits were not significantly different (p > 0.10) from FCA potentiated anti‐β‐hCG‐TT sera in neutralizing hCG‐induced uterine weight gain. C. parvum has potential for future application in active immunization studies of fertility regulation.