{'fraction_non_alphanumeric': 0.028616852146263912, 'fraction_numerical': 0.019077901430842606, 'mean_word_length': 5.070739549839228, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Protein digestion properties of Xinong Saanen goat colostrum and mature milk using in vitro digestion model.

BACKGROUND
Xinong Saanen goat milk is a raw material for goat milk based infant formula production. This study aims to analyze digestion properties of Xinong Saanen goat colostrum and mature milk by simulating infant gastrointestinal digestion. Zeta-potential, particles size, protein profile and peptides composition of these two kinds of milk during digestion process were studied.


RESULTS
Zeta-potential values of the digested colostrum were lower than those of mature milk through the whole digestion. Absolute zeta-potential of colostrum duodenal digestion samples showed a decrease from 16.63 ± 2.08 mV to 11.80 ± 2.03 mV while that of mature milk decreased sharply and then increased (p < 0.05). Colostrum had a larger particle size than mature milk and both milks showed decreased particle size with increasing digestion time but an increase for the last 30 min. Colostrum showed more high molecular weight (MW) proteins which cannot be hydrolyzed completely compared with mature milk. Digested peptides (< 10 kDa) were characterized using Liquid Chromatography combined with Tandem Mass Spectrometry (LC-MS/MS). The casein-derived peptides identified in digested colostrum and mature milk accounted for 76.67% and 59.53%, respectively. β-Casein was the most abundant in colostrum while that in mature milk was αs1 -casein. Enterotoxin-binding glycoprotein PP20K, Butyrophilin subfamily 1 member A1 (BTN1A1) and perilipin (PLIN) were only detected in digested mature milk.


CONCLUSION
Differences in digestion properties between goat colostrum and mature milk were mainly shown in duodenal digestion phase. Data may provide useful information about utilization of goat milk for infant formula formulation. This article is protected by copyright. All rights reserved.