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Survey of a laboratory building for airborne antibiotics.

Antibiotics are routinely examined by laboratory analysis in a six-story building which houses the laboratories of the National Center for Antibiotics and Insulin Analysis (NCAIA) on the second floor. A survey was performed to determine if antibiotic dust was being disseminated throughout the building. Air throughout the building was analyzed both quantitatively and qualitatively for residues of penicillin and tetracyclines. The microbiological assay plates used were sensitive enough to detect 0.001 unit of penicllin and 0.0004 mcg. of chlortetracycline in the air. Results of this study showed that, outside of the actual laboratories of NCAIA, the incidence of antibiotic contamination of the air was negligible. In the immediate areas where antibiotics were physically handled, 0.012 unit of penicillin and 0.0185 mcg. of chlortetracycline per cubic foot of air were detected.