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Numerical model for DNA loading in microdevices: Stacking and autogating effects

Many electrophoresis‐based DNA sequencing and genotyping microdevices rely on field‐driven effects to load and preconcentrate the sample. A quantitative model is developed for a broad class of electrophoresis‐based microfabricated sample injectors. Quantitative predictions of DNA preconcentration are compared with experimental data and are shown to qualitatively reproduce the detailed time‐evolving sample distribution in the injector. The model provides practical guidance on device and protocol design, in order to optimize this critical aspect of microfluidic devices.