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Verification of Triploid Golden Mahseer (Tor putitora) by Erythrocyte Measurement

The present study was focused on the conformity of the triploidy induction in golden mahseer. Erythrocyte measurement which is an indirect method for the conformity of triploidy induction in fish was followed for this purpose. Triploidy induction was carried out by pressure shock and thermal shock after 12 min of post-fertilization. The recorded results reflected 24.63% larger average size of erythrocytes cell in triploids (13.51 ± 1.13 μm) over the diploids (10.84 ± 1.01 μm). Similarly, the average measurement size of nucleus was recorded 21.27% lager in triploids (6.1 ± 0.61 μm) than the diploid counterpart (5.03 ± 0.42 μm). The size of erythrocyte cell was measured in the range between 8.4 and 13.28 μm for diploid and 10.4–15.39 μm for triploid while size of nucleus was recorded as for 4.24–6.12 μm for diploid and 4.73–7.37 μm for triploids, respectively. There was significant variation (P < 0.05) in major axis of erythrocytes cell and nucleus both in diploid and triploids individuals. Hence, the study showed the applicability measurement of erythrocyte cell as an indirect method for triploidy conformity in fish.