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Short Postspawning Recovery Time Affects Dna Integrity and Fertilization Success of South African Abalone (Haliotis midae) Oocytes

ABSTRACT 
 Hydrogen peroxide (H2O2) is used routinely by the abalone farming industry to induce spawning of adult abalone. The aim of the current study was to quantify DNA integrity of adult hemolymph cells as well as germ cells (oocytes and sperm) with different recovery times between spawning events. In addition, fertilization success of exposed germ cells was quantified. The results indicate that spawning events separated by short recovery (2–6 wk) will not provide sufficient recovery from H2O2-induced DNA fragmentation in adult hemocytes. It is recommended that the industry should ideally allow 6–8 wk between spawning events to prevent accumulation of DNA strand breaks in adults over time and to ensure adequate fertilization success to maximize farm production.