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TLC Image Capture and Analysis by use of a Prototype Device for Visualizing Fluorescence

This paper reports the evaluation of a novel, low-cost imaging device for use in TLC — a commercial flat-bed scanner modified by inclusion of a 366-nm UV light source. Images of native fluorescence were recorded after separation of a test dye. Video densitometry was performed on the digital data obtained and compared with slit densitometry measurements. The images obtained were high quality, easily processed and archived. The spatial and intensity resolution of the recorded images were greater than those recorded using current commercial TLC video documentation equipment employing analog CCD camera technology. Several observations were made. The linear range obtained by use of videodensitometric analysis was greater than that obtained from slit densitometry when a higher loading of analyte was used. The precision data for the whole analytical process were in close agreement when using a high loading of analyte whereas the converse was found at lower loadings. Detection sensitivity for the compounds studied were equivalent for the two techniques.