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Live-Cell Imaging of GFP in Plants.

The GFP from jellyfish Aequorea victoria is a very stable and relatively small protein of 238 amino acids. 
 
GFP serves as a molecular marker that can be imaged dynamically in living cells, both in its native form and as a fusion to other proteins. For GFP imaging, plants present the challenge of autofluorescence from chlorophyll, lignified cell walls, vacuolar contents, and other cell materials, all of which can obscure the GFP signal. Maximizing the signal-to-noise ratio is a major concern, and careful consideration should be given to the choice of tissue imaged, GFP expression level, GFP spectral variant (e.g., mGFP5), and selection of filter sets that block as much autofluorescence as possible. Root tips are among the best Arabidopsis tissues for live-cell imaging as they lack chlorophyll, are transparent, and can be grown on a microscope stage.