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Role of mixed‐function oxidases and non‐protein sulfhydryl contentent in [14C]‐2‐chloro‐4‐acetotoluidide binding to liver and kidney in starlings

The compound 2‐chloro‐4‐acetotoluidide (CAT) is highly toxic to many avian species, including the starling. In our earlier work, we demonstrated the covalent binding of radioactivity from [14C]‐CAT to liver and kidneys of the starling. In the present study, the effects of inducers of mixed‐function oxidase (MFO) and non‐protein sulfhydryl (NPSH) depletor on the total and covalent binding of [14C]‐CAT radioactivity to liver and kidney of the starling were examined. The total and covalently bound radioactivity from [14C]‐CAT to liver and kidney were decreased significantly in the starling pretreated with the MFO inducer, 3‐methylcholanthrene. However, pretreatment with phenobarbitaf, another inducer of MFO, had no effect. Pretreatment with the inhibitor of MFO, SKF 525‐A, reduced the covalent binding of [14C]‐CAT radioactivity to liver but not to kidney. There was no reduction in the NPSH content of liver or kidney following intravenous administration of CAT (3.5 mg kg−1). Reduction of NPSH levels in the liver or kidney following treatment with diethyl maleate caused a significant increase, in the covaient binding of [14C]‐CAT to kidney but not to liver.