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Analysis of prostatic fluid: Evidence for the presence of a prospective marker for prostatic cancer

In an endeavor to identify marker(s) for prostatic cancer, proteins in prostatic fluids were analyzed by two‐dimensional (2‐D) gel electrophoresis. The fluids were obtained from five males who had no prostate lesions and five patients each with benign prostatic hyperplasia (BPH) and prostatic carcinoma (PCA). The specimens were collected directly over a mixture of protease inhibitors and centrifuged, and the supernatants were lyophilized and solubilized in sodium dodecyl sulfate mix. Identical amounts of proteins were pooled according to donors' prostate disease and the resulting samples were subjected to 2‐D gel analysis employing the ISO‐DALT system. The electrophoretograms were developed by silver or double stain.