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Polarized structured illumination microscopy

Fluorescence polarization microscopy plays a vital role in studying molecular structures and dynamics of biocomplexes by imaging both the intensity and orientation of fluorescent dipoles. Super-resolution (SR) microscopy provides the ultrastructure of subcellular organelles, yet conventional SR lacks the dipole orientation information. We developed polarized structured illumination microscopy (pSIM) to achieve fluorescence super-resolution polarization microscopy.