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A Novel Enzyme Immunoassay Commonly Applied for Ten Strains of Pyricularia oryzae

Antiserum against a strain of the rice blast fungus Pyricularia oryzae was elicited in rabbits immunized with its cell fragments emulsified with incomplete Freund's adjuvant. The fragments were also used as solid‐phase antigens. A highly sensitive, competitive type enzyme‐linked immunosorbent assay for P. oryzae was developed by using these two preparations as the immune reagents together with the use of β‐d‐galactosidase‐labeled anti‐rabbit IgG as the tracer. Cross‐reactivity of nine different strains of P. oryzae were measured by the assay. Sensitivity and accuracy of the assay was improved by choosing the cell fragments of the least cross‐reactive strain as the solid‐phase antigen. The improved method was successfully applied for sensitive and accurate assay of all ten strains of P. oryzae with the common measuring range between 1 and 100 ng per tube. Other species of microorganisms had little reactivity in this immunoassay indicating that the assay is specific to P. oryzae group microorganisms.