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Isolation, molecular characterization and antibiogram of bacteria isolated from diabetic foot ulcers

Diabetic patients have increased risk of foot ulcers leading to minor or major amputation. This is habitually caused either by neuropathy or ischaemia, in few cases by both. Initial cause for such ulcers may be due to acute mechanical or thermal trauma or by repeated mechanical stress. Multidrug resistant aerobes have been closely related to diabetic foot infections. A high percentage of Staphylococcus aureus isolates were reported in hospitals associated with ulcer infections (Sanjay et al., 2013). In this study, swab samples from diabetic patients foot ulcers from hospitals in and around Chennai were collected. Out of 22 isolates, 4 strains showed high Multiple Antibiotic Resistant (MAR) index. 16S rRNA gene was amplified using PCR technique in all the 4 strains and they were characterized as Staphylococcus aureus, Morganella morganii, Acinetobacter baumannii and Acinetobacter Spp. Phylogenetic tree for each of the isolate was constructed to analyze its evolutionary relationship with closely related species.