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NOA 81 fabricated microfluidic chip for SH-SY5Y cell culture

Microfluidic chip has been an important tool for the study of life science, especially in cell biology researches. The chip provides precise manipulation of cells and helps establish similar environment in body network. A promising material, Noland Optical Adhesive 81 (NOA 81), was adopted here to fast fabricate the chip. The material was first cured and used for cell culture directly to test the biocompatibility and then fabricated into chips. We tested the flow influence on different designs of the chip structures. Finally, SH-SY5Y cells were induced and cultured in the microfluidic chip. The results show NOA 81 has good viabilities and adherence for cell culture. Column in chamber can reduce and disperse the fluid stress efficiently. With the efficient chip design, SH-SY5Y cells can survive in chip for more than one week.