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The effect of combined bleaching techniques on oral microbiota.

AIMS
To evaluate the antimicrobial activity of 10% and 37% carbamide peroxide during dental bleaching in three different modes.


MATERIALS AND METHODS
This five-week double-blind randomized controlled trial included 32 volunteers assigned to four groups (n = 8). Each group received bleaching agents or placebo as an in-office and at-home treatment. The dental bleaching techniques were: In-office bleaching (37% carbamide peroxide: CP37); at-home bleaching (10% carbamide peroxide: CP10) and the association of both (CP37 and CP10). Saliva samples were collected right before (baseline), right after, 12 hours after, and seven days after the treatment. Counts of total microorganisms, Streptococci, and Mutans streptococci were carried out. Friedman test (alpha = 0.05) was used to compare the microorganism counts.


RESULTS
The number of the all oral microorganisms remained stable during all experiment.


CONCLUSIONS
No bleaching agent (CP37, CP10 or the combination of both) was able to reduce the oral microorganisms tested.