{'fraction_non_alphanumeric': 0.025412960609911054, 'fraction_numerical': 0.010165184243964422, 'mean_word_length': 5.617647058823529, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Simultaneous reversal of age‐related declines in muscle health and cognition with transplantation of preconditioned mesenchymal stem cells (1163.2)

Mechanical strain can potently influence paracrine factor release from skeletal muscle‐derived mesenchymal stem cells (mMSCs). Since the MSC secretome has the potential to impact both local and distant tissue health, we assessed the extent to which transplanted mMSCs influence the local muscle environment as well as brain plasticity and behavior. mMSCs isolated from young muscle were preconditioned (10% multiaxial strain, 5 hrs) and immediately transplanted into the muscle of 24 month old mice. Mice receiving saline or non‐strained mMSCs served as controls. In addition to measuring indices of muscle health, the dentate gyrus of the hippocampus was evaluated for immature neurons one week post‐injection. Two weeks after transplantation, cognitive performance was measured by water maze, rotarod, and contextual fear conditioning. Although satellite cell number and fiber size were unchanged, vessel size and number of neuromuscular junctions were greater in muscle injected with strained mMSCs versus controls resulting in increased muscle function (P < 0.05). A significant increase in the number of immature neurons (P < 0.05) and a trend towards increased cognitive function on the water maze test was observed in these mice. Overall, mMSC preconditioning prior to transplantation may provide a novel method to prevent or reverse age‐related impairments in muscle strength, neuroplasticity, and cognitive function.