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Quantitation of plasma fibrinogen in the presence of fibrinogen degradation products.

Three methods for fibrinogen determination in plasma were compared as to their reliability in the presence of fibrinogen degradation products (FDP). The method of Clauss gave erroneously low values, the semiquantitative method of Schneider was less influenced by FDP, whereas the method of Jacobsson gave fairly accurate results. Additional experiments suggested that dilution of the test plasma was responsible for the very low values obtained with the method of Clauss, whereas the final pH of 6.4 in the method of Jacobsson was found to be critical for the limited effect of FDP in this assay.