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Measurement of human plasma renin substrate concentration by angiotensin I radioimmunoassay: a discussion of methodical problems.

Normal mean values of plasma renin substrate concentration reported in the literature vary widely (between 340 and 2286 Mg/1 angiotensin I-equivalents). Our own results suggest that plasma renin substrate concentration in healthy man (2283 ± 410 Mg/1 angiotensin I-equivalents) is higher than so far believed. The method described still measures plasma renin substrate values that are 7 % too low, owing to residual angiotensinase activity. During incubation of diluted plasma with human renin and inhibitors of angiotensinases and converting enzyme, only angiotensin I is formed. Angiotensin II is undetectable. We found considerable differences in the angiotensin I content between 3 different standard preparations investigated. Lack of an accurate angiotensin 1 standard and insufficient inactivation of angiotensinase seem to be the most important reasons for large differences between normal mean renin substrate-measurements from laboratory to laboratory. Methodical problems of substrate measurement are discussed and illustrated by experimental examples in this paper.