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Modulation in the rates of incorporation of lipid precursors during the cell cycle

The rates of incorporation of 3H‐choline into phospholipid and of 3H‐fucose into glycolipid have been measured during the cell cycle of the murine mastocytoma, P815Y, synchronized by either velocity sedimentation or excess thymidine blockade. The rate of 3H‐choline incorporation into acid‐insoluble material exhibited two distinct maxima coincident with the early S and G2 periods, whereas the rate of incorporation of 3H‐fucose into lipid extractable material was maximal during the G2 period. Variation in rate of incorporation of 3H‐choline could not be accounted for by changes in membrane permeability.