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Screening of Chitosan Derivatives-Carbon Dots Based on Antibacterial Activity and Application in Anti-Staphylococcus aureus Biofilm

Introduction Pathogenic bacteria, especially the ones with highly organized, systematic aggregating bacteria biofilm, would cause great harm to human health. The development of highly efficient antibacterial and antibiofilm functional fluorescent nanomaterial would be of great significance. Methods This paper reports the preparation of a series of antibacterial functional carbon dots (CDs) with chitosan (CS) and its derivatives as raw materials through one-step route, and the impact of various experiment parameters upon the optical properties and the antibacterial abilities have been explored, including the structures of the raw materials, excipients, and solvents. Results The CDs prepared by quaternary ammonium salt of chitosan (QCS) and ethylenediamine (EDA) exhibit multiple antibacterial effects through membrane breaking, DNA and protein destroying, and the production of singlet oxygen. The CDs showed excellent broad-spectrum inhibitory activity against a variety of bacteria (Gram-positive and negative bacteria), in particular, to the biofilm of Staphylococcus aureus with minimum inhibitory concentration at 10 µg/mL, showing great potential in killing bacteria and biofilms. The biocompatibility experiments proved that QCS-EDA-CDs are non-toxic to human normal hepatocytes and have low haemolytic effect. Furthermore, the prepared QCS-EDA-CDs have been successfully used in bacterial and biofilm imaging thanks to their excellent optical properties. Conclusion This paper explored the preparation and application of functional CDs, which can be used as the visual probe and therapeutic agents in the treatment of infections caused by bacteria and biofilm.

CDs have shown great potential applications in biomedical fields, and their antibacterial ability has also arisen great interests of researchers. Normally, CDs could affect physiological functions of bacteria and finally kill them through mechanisms like oxidative stress, 9 cell membrane damage, 10 induction of gene apoptosis, 11 adsorption and encapsulation. 12 However, CDs do not show effective inhibitory ability towards biofilms regardless of their strong ability to planktonic bacteria. 15 Li and his team 16 prepared a nanosystem based on CDs, whose minimum inhibitory concentration (MIC) against Staphylococcus aureus (S. aureus) was 250 μg/mL. However, when treating biofilm, its MIC rose to 1000 µg/mL. Therefore, the development of CDs with effective ability against biofilm is of great significant to clinical application.
For the presents, CDs destroy biofilm in two ways: to kill the resident bacteria to degrade the biofilm, or to directly destroy the materials on the biofilm, such as proteins, to affect its formation. For example, Liang et al 15 used tinidazole to prepare tinidazole CDs (TCDs), and the prepared TCDs could inhibit the growth of P. gingivalis with MIC at 50 μg/mL and completely inhibit the formation of biofilm at 100 μg/mL. Singh and his team synthesized biomass CDs with curcumin as raw material, and CDs could interact with matrix proteins and thus possess anti-biofilm ability through biofilm degrading behavior. 17 However, reports on anti-biofilm CDs are still limited, and the antibiofilm mechanisms need further exploration.
The excellent properties of CDs are the basic requirement for their applications in antibacterial and antibiofilm field. Traditional antibacterial function of CDs was realized through coupling, 18 but the one-step route to prepare antibacterial CDs would be more attractive due to its simple operation, environmental-friendliness, low cost and stable emission. 19 It was discovered that the inhibitory behavior of prepared CDs were influenced by the types of precursors, excipients (including carbon source, nitrogen source, sulfur source, reducing agent, passivator, doping agent and acid-base regulator, etc.) and the preparation environment parameters. Analysis and filtration for raw materials, especially the ones of the same type or of the different structure domains are crucially meaningful in CDs studies. Meanwhile, the excipients and the optimization of synthesis process could also adjust the electron density and group composition, and thus the properties of the product, including the particle size and optical properties, further affecting the biochemical behavior in their practical applications. For instance, the cationic CDs could easily penetrate through or aggregate on the biofilm, 20 so chitosan (CS) with cationic group on the surface is an ideal raw material to prepare cationic antibacterial CDs. 21 Travlou et al discovered that compared with S-doped CDs, N-Doped CDs exhibited higher antibacterial ability, which was relevant to the specific surface chemical properties and particle size. 22 Huang and his team discovered that the MIC of the prepared spermidine CDs (prepared at 260°C) against methicillin-resistant Staphylococcus aureus (MRSA) is at least ten times lower than that of CDs prepared at other temperatures. 23 The above researches provided meaningful basis for designing high-efficient antibacterial materials. Notably, with extraordinary optical properties and biocompatibility, CDs have also been used in microorganism monitoring. 24 The research introduces steps to prepare blue-fluorescent CDs with chitosan (CS) and its derivatives as carbon sources through one-step hydrothermal route. The impacts of raw materials and their structures, excipients and solvents towards the antibacterial ability in prepared CDs are presented, and numerous characterization methods are adopted to study the reasons to the antibacterial behaviors of different CDs. The experiment results showed that CDs (QCS-EDA-CDs) prepared by QCS and ethylenediamine (EDA) exhibited strong inhibitory ability to several types of bacteria. Scanning electron microscope and molecular biology methods have been used to explore the impact of QCS-EDA-CDs to the growth of the bacteria. Furthermore, optical experiments showed the induced reactive oxygen species (ROS) under daylight lamp, indicating the antibacterial ability is based on multiple mechanisms. Meanwhile, CDs also exhibited strong inhibitory behavior to the biofilm of Staphylococcus aureus (S. aureus), including the formation process of biofilm and mature biofilms. The prepared the prepared QCS-EDA-CDs have low toxicity in biocompatibility evaluation, which making them ideal material as multi-colored fluorescent probe to S. aureus cells and biofilm imaging.

Material Characterizations
Fluorescence spectra were measured by LS55 fluorescence spectrophotometer (PerkinElmer). Absorption spectra of Ultraviolet-visible (UV-Vis) were recorded using Lambda-35 UV-vis spectrophotometer (PerkinElmer). The transmission electron microscopy (TEM) and high-resolution TEM (HRTEM) images were analyzed using JEM-1400Plus transmission electron microscope (Japan Electron Optics Laboratory Co., Ltd). Zeta potentials for CDs were evaluated by zetasizer (Nano ZSE, Malvern Instruments, UK). VG Multilab 2000 X-ray photoelectron spectroscopy was gathered by surface analysis. Optical density (OD) in the cell was calculated using microplate reader (Thermo Scientific, England, UK). The circular dichroism spectra were gathered using Chirascan Plus spectropolarimeter (Applied Photophysics Ltd.). Scanning electron microscope (SEM) images for bacteria were conducted using Zeiss SIGMA scanning electron microscope (Carl Zeiss Jena). Fluorescence optical microscope (Nikon ECLIPSE Ti) was applied on images through laser-scanning confocal fluorescence.

Synthesis Method of CDs
The synthesis procedure of QCS-EDA-CDs was as follows: 200 mg of QCS was dissolved in 200 μL EDA and 20 mL DI water and the mixture was afterwards moved to Teflon-lined stainless-steel autoclave and preserved at 200°C in 4 h. Synthesis methods of other CDs are in the Supplementary Materials.

Inhibition of Biofilm Experiments
Fresh S. aureus culture (10 9 CFU mL −1 ) was diluted 1:10 in 200 μL TSB medium, which contained QCS-EDA-CDs at varying concentrations (5,10,15,20,25,30,40 μg/mL) on 96-well plates. Wells with no QCS-EDA-CDs were used for control. The mixture was cultured at 37°C. The OD value of per well was measured using the microplate reader at 600 nm within 48 hours. The time curve of OD 600 at different concentrations was drawn.

Live/Dead Cell Imaging
To evaluate the inhibitory effect of the sample on the formation of biofilms, fluorescence photography was performed using the confocal laser scanning microscope (CLSM). Upon incubation, the S. aureus biofilm was rinsed thrice in PBS (pH = 7.4). It could be detected on CLSM after staining with DAPI/PI (10 µg/mL) for 15 minutes. The fluorescence image was obtained when the sample was simultaneously excited with 350 and 533 nm, and the corresponding emission was at 461 and 615 nm, showing blue and red, respectively.

SOSG Oxidation in the Presence of 1 O 2
The reaction between SOSG and SOG produced from photoirradiation for QCS-EDA-CDs (1 mg/mL) in PBS was investigated. SOSG was added with a concentration of 2.5 µM. The control groups include: (1) Pure SOSG; (2) pure CDs. The tested solutions were irradiated with daylight lamp or placed in the dark for 30 minutes. The SOG was generated from irradiation at 671 nm. The SOSG fluorescence was recorded under the excitation at 494 nm, and the maximum was detected upon irradiation to decide sample SOG. Sample SOG was assessed through SOSG fluorescence enhancement in comparison with the background or control sample. 25

Results and Discussion
The Impact of Different Conditions Upon the Antibacterial Ability of CDs The Effects of Raw Material Type on CDs CS and its derivatives (carboxymethyl chitosan (CMCs) and oligochitosan quaternary ammonium salt (QCS)) have been used to prepare CDs, to study the impact of carbon source domain to the inhibitory abilities of prepared products. CS-CDs, QCS-CDs and CMCS-CDs have been prepared through one-step hydrothermal route (200°C, 4 h) (Scheme 1) with CS, CMCS and QCS as raw materials respectively. Since CS is slightly soluble in water, 1% acetic acid was used as the solvent when CS used as raw material. The antibacterial abilities of three prepared CDs against S. aureus are studied to explore the impacts of raw material structure to the antibacterial abilities of the products. According to Table 1, the minimum inhibitory concentrations (MIC) of CS-CDs, CMCS-CDs and QCS-CDs against S. aureus are 250, >1000 and 10 μg/mL. CMCS-CDs show almost no antibacterial ability to S. aureus, while QCS-CDs have the best antibacterial ability.
Scheme 1 shows the structural domain differences of CS and its derivatives. The CMCS and QCS are prepared by using carboxymethyl and 2,3-epoxypropyl trimethylammonium chloride to replace the hydrogen atom in amino group of CS. Some residual groups of these three raw materials still exist on the surface of prepared products after high temperature, dehydration, nucleation and carbonization. Since antibacterial abilities of the raw materials might be inherited into their products, the electrical properties of these three raw materials and their products are investigated to explore the reasons to the difference in antibacterial abilities ( Figure S2). The MICs of CS and QCS against S. aureus are both 25 μg/mL, while CMCS almost does not have any antibacterial ability. The experiment results show that CMCS and its product CMCS-CDs are both negatively charged, which would prevent their interactions with negative-charged bacteria. The positive charge of CS and QCS (zeta potentials: +65.8 and +59.1 mV) and their products CS-CDs and QCS-CDs (zeta potentials: +32.9 and +30.7 mV) promises their interactions with bacteria, penetrating cell membrane and finally killing the bacteria. Since QCS-CDs exhibit better water solubility and stronger antibacterial ability than other prepared CDs, it is thus chosen as the target of further studies.

The Influence of Excipients on the Synthesis of CDs
As is known that besides carbon source, other synthesis parameters, including the nitrogen source, sulfur source, reducing agent, passivator, doping agent and acid-base regulator, could also influence the prepared CDs' optical properties, but their influences upon the antibacterial behaviors of the products are often neglected. 26 The impacts of common auxiliary materials CA (50 mg) and ethylenediamine (EDA, 200 μL) upon the prepared QCS-CDs' properties are investigated. According to Figure 1A and B, UV-vis absorption peaks in QCS-CA-CDs and QCS-EDA-CDs are at 270 nm and 304 nm, attributed to the electron transition from σ and π orbits (occupying the highest molecule). At the excitation wavelength of 370 nm, their emission wavelengths reach 465 nm and 466 nm, almost the same as that of QCS-CDs (467 nm), showing that their luminescent groups are mainly from the carbon source QCS. Moreover, the QYs of QCS-EDA-CDs is calculated as 9.0% with quinine sulfate as reference, higher than that of QCS-CDs prepared with water as solvent (QYs=0.9%). The high fluorescence QY of EDA-passivated CDs may be attributed to the interplay between the holes trapped and the passivation of the CDs. 27 According to Table 1, the MIC in QCS-CA-CDS against S. aureus is 125 μg/mL, much lower than that of QCS-CDs (10 μg/mL), while the MIC of QCS-EDA-CDs is 10 μg/mL, similar to that of QCS-CDs.

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Several characterization means have been employed to compare their functional groups of these CDs to study their antibacterial mechanisms. As shown in FTIR spectra ( Figure 1C), the peak of QCS-CA-CDs at 1375 cm −1 in infrared absorption spectra results from the in-plane bending in -OH bonds, while the peak of QCS-EDA-CDs at 1063 cm −1 is attributed to the vibrations of C-O bonds. 28 Besides the absorption peak of −N + (CH 3 ) 3 (1488 cm −1 ), QCS-CA-CDs also have absorption peaks of −COOH at 3398 and 1697 cm −1 . 29 The measured zeta potentials of these two CDs exhibit obvious difference. The zeta potential of QCS-CA-CDs is +11.7 mV ( Figure 1D), lower than that of QCS-EDA-CDs (+20.6 mV). When CA is used as carbon source in preparation, the carboxyl group in the structure of CA can react with quaternary ammonium group in QCS, leading to reduced zeta potential. The changed surface group and reduced zeta potential would decrease the binding ability of prepared CDs with negatively charged bacteria, and thus its antibacterial abilities.

The Impact of Solvent Upon the Antibacterial Ability of CDs
Though the reaction solvent has been regarded as the crucial synthesis parameter for optical properties of prepared products, 30,31 the impact of solvent upon the antibacterial ability of prepared CDs still needs detailed research. Water and organic solvent FO are used as solvent to prepare QCS-CDs and QCS-FO-CDs. As shown in Figure S3a, the absorption peak of QCS-FO-CDs in UV-vis absorption spectrum reaches 271 nm, and emission wavelength reaches 449 nm at the excitation wavelength of 370 nm, shorter than the emission peak of QCS-CDs.
With S. aureus as target bacteria, QCS-FO-CDs exhibit almost no antibacterial ability against bacteria (MIC>1000 μg/mL), much weaker than QCS-CDs prepared in water. The enhancement of other functional groups in CD skeleton,

Characterization of QCS-EDA-CDs
Several characterization methods are adopted to investigate QCS-EDA-CDs' properties. The emission wavelength of QCS-EDA-CDs increases from 340 nm to 390 nm as corresponding excitation wavelength increases gradually from 439 nm to 468 nm (Figure 2A), showing its excitation wavelength dependent property. The morphology of prepared QCS-EDA-CDs is presented with TEM. According to Figure 2B  As shown in Table 2  The prepared QCS-EDA-CDs have been compared with other chitosan-derived nanoparticles and quaternized CDs reported in other references, in aspects of preparation methods and antibacterial properties ( Table 2). QCS-EDA-CDs show much better antibacterial behavior against S. aureus than chitosan nanoparticles (CS NPs), carvacrol-grafted chitosan nanoparticles (CSCA NPs) and eugenol-grafted chitosan nanoparticles (CSEU NPs) whose MICs against S. aureus are beyond 500 µg/mL. 35 Though the MIC of QCS-EDA-CDs is quite close to that of quaternized CDs, QCS-CDs reported in this paper require simpler and easier synthesis process.
Since Gram-negative bacteria are in an outer membrane structure that can be hardly penetrated and protect bacteria from the antibiotics, the infection caused by Gram-negative bacteria is harder to be cured than that caused by Grampositive bacteria. The above-mentioned references also reported the antibacterial ability of chitosan nanoparticles (CS NPs, CSCA NPs and CSEU NPs) against E. coli, 35 with their MIC at 0.5-1, 0.5-1 and 0.25-0.5 mg/mL respectively. QCS-EDA-CDs exhibit similar inhibitory ability as those reported nanoparticles.
The structural difference of these two types of bacteria might be the reason to such difference in exhibited antibacterial abilities of QCS-CDs. It is found that Gram-positive bacteria show greater sensitivity towards lipophilic molecules compared with Gram-negative bacteria. The cell wall of Gram-positive bacteria (such as S. aureus) and Gramnegative bacteria (such as E. coli) is in different chemical composition. 38 S. aureus and the corresponding bacteria have porous cell wall because of cross-linked peptidoglycan's distributed thick layer on the plasma membrane, which may promote QCS-EDA-CDs' interaction with S. aureus and MRSA, and thus encourage the antibacterial behavior of CDs. 39 However, the cell wall of E. coli contains an outer membrane and intermittently peptidoglycan network, hindering the interaction between CDs and E. coli and making the bacteria exhibited stronger resistance against the inhibitory behavior of CDs.

Inhibitory Abilities of QCS-EDA-CDs Against Biofilm
The inhibitory ability of prepared QCS-EDA-CDs against the bacterial biofilm is tested with pathogen S. aureus as the target bacteria. Compared with control group, QCS-EDA-CDs can significantly decrease biofilm amount in S. aureus (Figure 3), and the inhibition rate against biofilm is positively related to every antibacterial agent's concentration. When

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Nowadays, some antibacterial nanoparticles with the activity of inhibiting biofilm have been developed. 16,[40][41][42][43][44][45] However, they often have some defects, such as too large antibacterial MIC or inhibiting biofilm in a much larger amount than MIC in a short period of time, which is not conducive to the application of antibacterial materials in actual medical treatment. With reference to previous studies (such as Table 3), QCS-EDA-CDs have long-term biofilm inhibition activity, and their MBIC and MIC are not much different. This shows the superiority of QCS-EDA-CDs in inhibiting biofilms.
Recent researchers have discovered the negative activity of chitosan towards the formation of biofilm of a variety of bacteria and fungi. 45 Chitosan can inhibit the formation of biofilm, and lead to the dispersion of pre-formed biofilm. The confocal laser scanning microscope (CLSM) images of Figure 4 further show the impact of QCS-EDA-CDs upon the formation of the biofilm of S. aureus. Under the low concentration of QCS-EDA-CDs (250 μg/mL) (Figure 4), the biofilm shows compact structure and the internal bacterial cells are well clustered, whereas under high concentration of QCS-EDA-CDs environment, the density of biofilm has significant decrease and the bacterial debris can be obviously found. The images prove the inhibitory effects to the growth and reproduction of biofilm, as well as to the mature biofilm.
To get more directly assessment of the impact of QCS-EDA-CDs upon the mature biofilm of S. aureus, CLSM is also used to observe the PI/DAPI-labeled biofilms of S. aureus treated by QCS-EDA-CDs at different concentrations. DAPI and PI are common staining agents for live and dead cells. The wall/membrane-damaged bacteria would be dyed as red-colored, and the wall/membrane intact bacteria cells as blue-colored. As shown in Figure 5A-C, the untreated biofilm of S. aureus exhibits blue fluorescence without any red fluorescence. In contrast, after the treatment of QCS-EDA-CDs, the biofilm shows strong red fluorescence, illustrating the death of large amount of bacteria. Meanwhile, the biofilm treated by two concentrations of QCDs-EDA-CDs for the same duration of time ( Figure 5) shows the gradual increase red fluorescence, and under the light field, the dense biofilm structure can be observed being destroyed. The result shows that the high concentration of QCS-

Antibacterial Mechanism of QCS-EDA-CDs
The antibacterial mechanism of QCS-EDA-CDs is explored with S. aureus as the model bacteria. As shown in SEM images ( Figure 6A), the mature bacterial biofilm shows aggregation of complete cells connected and coated with EPS, and the bacterial cells are full. After being treated by QCS-EDA-CDs for four hours, the biofilm shows obvious decrease in its density, and the decrease in bacteria amount ( Figure 6B). The bacterial cells are wrinkled, and the surface are seriously damaged and collapsed. The wrinkled and collapsed shape of bacteria prove the disruption of membrane integrity induced by QCS-EDA-CDs, while in control group, bacteria in normal cultivation condition have a spherical structure. The result shows that QCS-EDA-CDs kill bacteria mainly through rupturing its cell membranes.
The prepared QCS-EDA-CDs have been proven to possess excellent antibacterial capacity towards Gram-positive and negative bacteria. Considering the crucial role of QCS-EDA-CDs' surface-chemical properties in interaction activity  46 It is concluded that QCS-EDA-CDs realize the antibacterial activity through destroying the cell membrane and decomposing EPS. The small size and the hydrophobic organic chain of QCS-EDA-CDs make them easy to enter the substrate layer of biofilm, and the rich quaternary ammonium cations on the surface of CDs would interact with biofilm, destroying the matrix structure of biofilm and finally destroy the bacterial cells (Scheme 2).
Photodynamic therapy (PDT) belongs to a conventional therapy against bacteria. With oxygen, photosensitizer transfers absorbed photon energy to oxygen molecules nearby, and generates reactive oxygen species (ROS) with 1 O 2  47 SOSG has interactions with 1 O 2 to generate SOSG endoperoxides (SOSG-EP) and releases intense green fluorescence at the maximum intensity of 540 nm. 48 The daylight lamp could be used as the light source. As shown in Figure S5, in contrast to pure SOSG (2.5 μM) group, the solution added with the presence of QCS-EDA-CDs exhibit obviously stronger fluorescence intensity, showing the formation of 1 O 2 . The pure QCS-EDA-CDs do not exhibit any fluorescence at 540 nm after the illumination of 30 min, illustrating that the fluorescence intensity change originates from the combination of SOSG and reactive oxygen species.
Circular dichroism spectroscopy can be considered a meaningful approach to detect the secondary structure for DNA and proteins in the solution. 49 It was used to examine the impact of QCS-EDA-CDs on DNA. As shown in Figure 7A, in comparison with the control group, the decline of QCS-EDA-CDs group's peak suggests that QCS-EDA-CDs loosen DNA's double helix structure. Because of the nanoscale size, QCS-EDA-CDs penetrate into the bacterial cell upon penetrating the cell membrane, and loosen the naked DNA structure of bacteria, thus hindering the proliferation of bacteria. Total protein of S. aureus is retrieved using the bacterial total protein extraction kit, and circular dichroism spectroscopy is applied in the study on the interaction of QCS-EDA-CDs with the total protein of S. aureus. Without the presence of QCS-EDA-CDs, there exists a broad absorption peak at about 220 nm as the result of the n→π* transition in the α-helical structure ( Figure 7B). 50 After the incubation with QCS-EDA-CDs, the weakened CD signal proves the reduced α-helical structure of protein because of the interaction between CDs and protein, leading to the change in hydrogen bond network and secondary structures of proteins, and consequently the damages of bacteria activities. Upon protein incubation with QCS-EDA-CDs, three new protein absorption peaks are found at 202 and 203 nm, suggesting the presence of QCS-EDA-CDs influences protein structural characteristics. The experimental results verify the antibacterial activity of QCS-EDA-CDs is through changing the protein and DNA structure for bacteria.
Alternatively, as shown in Scheme 2, QCS-EDA-CDs damage cell membranes, intracellular protein and DNA, eventually resulting in bacterial death to exert antimicrobial effects. Admittedly, for better understanding the specific targets and detailed mechanism of QCS-EDA-CDs for bacteria, other related experiments and technologies should be also verified, such as the investigation on the anti-quorum sensing activity of CDs as fungal crude extracts, 51 to adjust the biofilm formation.

Biocompatibility Assay of QCS-EDA-CDs
Cytotoxicity is regarded as one of the important factors that determine the safety of biomedical materials, and the cytotoxicity and blood compatibility of QCS-EDA-CDs in the cell are therefore detected. LO2 cells are used as the target  Figure 8A shows the proliferation rates of cells cultured at different doses of QCS-EDA-CDs in 48 h. MTT assay reveals that average cell viability exceeds 97% when QCS-EDA-CDs concentration is at 200 µg/mL. The finding promotes QCS-EDA-CDs as ideal candidates to use in cellular imaging for diagnosis purposes.
Moreover, an in vitro hemolysis assay is conducted on defibrinated human blood to explain the biocompatibility of QCS-EDA-CDs and QCS of red blood cells (RBCs) in biological solution. Spectrophotometric analysis on supernatants and photographs for treated RBCs are gathered after exposing RBCs to QCS-EDA-CDs and QCS at different concentrations in 3 h ( Figure 8B). Notably, the supernatant in the RBC suspension remains clear, with a measured concentration of 500 µg/mL. When the sample concentration exceeds 500 μg /mL, the hemolysis rates in QCS-EDA-CDs and QCS are less than 0.08% and 1.06%. A hemolysis rate below 5% is secure in biomedical applications. This result indicates that QCS-EDA-CDs do not lead to the rupture and hemolysis in red blood cells in physiological conditions and shows excellent blood compatibility.

Conclusion
In summary, with CS and its derivatives as raw materials, a variety of blue-fluorescent CDs are prepared through simple and fast one-step hydrothermal route, to study how carbon source affected CDs in optical properties and antibacterial activities. Through carefully selecting proper raw material type and synthesis parameters, QCS-EDA-CDs with high antibacterial ability are prepared. Numerous characterization means are adopted to examine the antibacterial mechanism and process of QCS-EDA-CDs against bacteria. The experiments show that the rich quaternary ammonium groups on QCS-EDA-CDs make CDs strong positively charged, leading to easy combination with the negative charges on the surface of bacterial cells. QCS-EDA-CDs could destroy the protein and the secondary structure of DNA, cause the disorder of normal functions, and finally kill bacterial cells. Meanwhile, QCS-EDA-CDs have been proven to induce single oxygen under daylight lamp and could realize antibacterial effect through multiple mechanisms. The prepared CDs could also inhibit the growth of biofilm of S. aureus. Due to their excitation wavelength dependency property, QCS-EDA-CDs have been applied in the imaging of bacterial cells and biofilm. Cytotoxicity and hemolysis tests also show their excellent biocompatibility. These results show the potential application values of QCS-EDA-CDs in novel fluorescence labeling, biomedical imaging and visual treatment of bacterial infection.