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[The study of TNF-alpha destructive action to the bone in human middle cholesteatoma].

OBJECTIVE
To study the expression of TNF-alpha in the cholesteatoma and elucidate the role of TNF-alpha in the destruction of bone.


METHOD
All samples (5 microns paraffin sections) from 25 cholesteatoma cases and 10 normal cases were examined by immunohistochemical SABC method and analysed by computer image.


RESULT
In 25 cholesteatoma cases, TNF-alpha expressed in the cytoplasm of the full epithelial tissue cells, subcutaneous inflammatory cells and fibroblast. In contrast, the normal cases, there were 6 cases week positive, 4 cases negative. The results of the computer image quantitative analysis system showed that the mean optical density of TNF-alpha was 0.1326 +/- 0.0022 in the cholesteatoma epithelial tissue and 0.0868 +/- 0.0014 in normal skin epithelial tissue respectively, both of which had significantly differentiation (P < 0.05).


CONCLUSION
TNF-alpha may be a factor of the destruction of bone of cholesteatoma.