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In vitro determining the effect of vaginal lactobacillus on some pathogenic of urogenital infections in women

It has been postulated that lactobacillus play a critical role in maintaining the normal vaginal ecosystem by preventing overgrowth the of pathogens and other opportunistic organisms by producing lactic acid hydrogen peroxide, bacteriocines and other antimicrobial substances. Current study aimed to determine the effect of vaginal lactobacillus spp (L.acidophilus, L. crispatus, L. gasseri and L. iners), as probiotics isolated from vaginal swabs of fifty healthy women for reduction of urogenital infections(Candida albicans, Gardenella viginals, Staphylococcus aureus and Escherichia coli) isolated from female patients who attended the obstetric and gynecology clinics in Ibn-AL Baladi Hospital. The effects of lactobacillus strains supernatants agents urogenital pathogens were tested in agar plate diffusion method on Manns-Regoz and Sharpe (MRS) agar and in liquid medium (MRS broth).Also Minimum inhibitory concentration (MIC), Minimum bacteriocidal concentration(MBC) and Minimum fungicidal concentration(MFC) were determined. The result of antimicrobial activity of vaginal lactobacillus revealed that L.crispatus had the highest effect on urogenital pathogens in both methods while the result of MIC , MBC and MFC showed that the concentration 60% led to minimized growth of G. viginals, S. aureus and E. coli and concentration 70% inhibit growth of these bacteria completely , whereas sharp decrease in candida albicans growth noticed in80% concentration even as the concentration 90% and above inhibited all pathogenic isolates (MBC and MFC).From above results it be clear that all Lactobacillus strains have antimicrobial activity against urogenital pathogens but L.crispatus had the highest affect than others with promising inhibitory spectrum.