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Herpesvirus saimiri (HVS)-transformed T-cell lines: A method to study mucosal T cells in inflammatory bowel disease

Alterations in the mechanisms regulating the immune response are key elements in inflammatory bowel disease (IBD, comprising Crohn’s disease (CD) and ulcerative colitis (UC)). Although the precise aetiology remains unknown, T lymphocytes play a crucial role in its pathogenesis. Initially, descriptions of the immunological features in IBD focused on peripheral blood lymphocytes. Since these data do not necessarily reflect the situation at the mucosal milieu, it is important to assess the phenotypic profile and activation state of immune cells at this level. We have used a common lymphotropic virus of squirrel monkeys, Herpesvirus saimiri (HVS), to immortalize T cells of intestinal mucosal origin from patients and healthy individuals as controls. HVS-transformed T lymphocytes become antigenand mitogen-independent for their continuous growth and upon stimulation with membrane or transmembrane stimuli, these cells show normal downstream functional responses [1,2].