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SELECTIVE SYSTEM IN VITRO «FUNGI COLLETOTRICHUM LINI - LINEN» AS AN EFFICIENT WAY TO FIND OUT GENOTYPES RESISTANT TO THE POD SPOT

The research aims at development of an effective selective agent in vitro system for founding linseed genotypes resistant to the pod spot. The authors see the object of research as varieties and lines of flax Linum usitatissimum L., which differ in their resistance to the pod spot. Fungi strains differed in their virulence. The authors applied methods of such scientists as Dospekhov and Kurchakova, methodological guidance on foundation, maintenance, storage and practical application of microorganisms, i.e. flax pathogens. This results in creation of selective in vitro system “Colletotrichum lini Manns et Bolley fungus – flax”. This system selects in vitro flax cells resistant to culture filtrate, from which it is possible to obtain regenerated plants resistant to the pathogen with greater efficiency. The authors enumerate the aminoacids that were found in the culture filtrates of the investigated pathogen strains; they are alanine, glycine, asparagine, cysteine, asparagine and glutamic acids, arginine and threonine. The authors outline the observed relationship between flax cell responsiveness and fungi pathogen in the environment of the fungius - anthracnose pathogen - on the value of the explant. Anthers cells in selection conditions were less resistant than those of immature embryos. The researchers observed the impact of flax genotype on cells ability to morphogenesis under selection conditions. Genotype cells L 957-8-7, Alexim, Pendzhab, Zaryanka had high morphogenetic activity. Morphogenetic capacities of genotypes L 1506-8-4 and Rosinka were rather low by the 2nd-3rd passages. When designing the scheme of flax selection in vitro for resistance to anthracnotism, 86 shoots were obtained, the check of which on the artificial infectious-provocative background showed that the genotypes differed in their resistance. The authors observed forms less resistant to the disease as well as resistant and medium resistant lines (at the level of 50 - 75%). The parameters of resistance in resistant and medium resistant genotypes were 12 - 37% higher than in the initial forms.