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148B: KILLING MECHANISM OF ONCOLYTIC DESIGNER HOST DEFENSE PEPTIDE - NECROSIS OR APOPTOSIS?

Material and Methods: In vitro the human liposarcoma cell line SW-872 was exposed to [D]-K3H3L9, a 15-mer D,L-amino acid designer peptide. Genotoxic activity of this peptide (dose: 0-62,5μM) was measured using TUNELassays. FACS-analysis allowed to discriminate dead, viable and apoptotic cells after 2, 15 and 30 minutes of peptide incubation (dose: 62,5μM). Via immunohistochemical staining the peptide distribution within the cell was analysed after 0, 1, 60 minutes and 24 hours (dose: 62,5μM) of incubation. Morphological changes were evaluated using microscopy and confocal laser scanning microscopy. In vivo [D]-K3H3L9 was administered intratumorally in a SW-872 xenograft mouse model. Tumor sections were stained with toluidine blue and microscopically analysed with respect to apoptotic and necrotic effects.