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BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice

BIG1 and its effector Arf1 are newly added to the list of signaling molecules controlling axonal myelination.


INTRODUCTION
During embryonic development of the peripheral nervous system in mammals, Schwann cell lineage cells migrate along peripheral neuronal axons from the dorsal to the ventral direction, arriving at their final destinations such as the limbs. After birth, they begin to wrap around individual axons and myelinate axons until~2 months. Mature myelin sheaths play essential roles not only in insulating axons to increase nerve conduction velocity but also in protecting them from various external stresses such as physical ones. In the peripheral nervous system, myelin sheaths are derived from Schwann cells' morphologically differentiated plasma membranes. They often grow to more than 100 times larger than the collective surface areas of the premyelinating Schwann cell plasma membranes (1)(2)(3)(4)(5). As such, whereas myelin functions have been well studied, molecular units controlling the Schwann cells as they undergo such dynamic morphological changes, especially at the intracellular level, are not sufficiently understood.
Intracellular signaling molecules that control cell morphological changes involve a family of small guanosine triphosphatases (GTPases). They are active when bound to guanosine triphosphate (GTP) and are inactive when bound to guanosine diphosphate (GDP). Two regulatory molecules that are needed to behave like GTPases are signaling switches. Guanine nucleotide exchange factors (GEFs) replace GDP with free cytoplasmic GTP on GTPases to generate active GTPases. In contrast, GTPase-activating proteins promote intrinsic GTPase activities to generate inactive ones (6)(7)(8)(9)(10)(11). Thus, a rate-limiting step to activate GTPases is thought to lie in their cognate GEFs.
Thus, the long-standing question of whether Arf1 and Arf1's GEF actually correlate to myelin sheath formation has been left unanswered. Here, we have generated conditional knockout mice for Arfgef1 [generally known as brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1)] and have investigated whether BIG1 is involved in the regulation of the initiation of myelination by Schwann cells. Compared with littermate controls, Schwann cell-specific BIG1 knockout mice result in decreased myelin thickness in sciatic nerves, especially in the initiation of myelination. The knockout mouse nerves exhibit a specific decrease in the amounts of Arf1 in an AP1 but not in those of Arf1 in a COPI. The binding of Arf1 to GGA1 is comparable in BIG1 knockout mice and controls. Furthermore, we have generated conditional Arf1 knockout mice, which have produced similar results in myelin thickness with BIG1 knockout mice. These results illustrate key roles for BIG1 and Arf1 in peripheral nerve system myelination, presenting previously unanticipated molecules involved in myelination.

RESULTS
BIG1 is up-regulated during the peripheral nervous system development To examine changes in expression of BIG1 and related molecules during development of the peripheral nervous system, we used mouse sciatic nerves and carried out immunoblotting with the respective specific antibodies. Expression levels of BIG1 increased until 7 days after birth. Elevated expression levels were very clearly detected until~1 month, after which these levels decreased ( fig. S1, A and B). BIG1 begins to upregulate when myelination is initiated. On the other hand, levels of the BIG1 effector Arf1 and control proteins were comparable throughout the ages of mice used for immunoblotting. As mice developed, the expression levels of myelin protein zero (MPZ), the major peripheral myelin membrane protein, were increased.
BIG1 regulates the initiation of myelination in the peripheral nervous system Thus, we investigated the effect of knockout of BIG1 in Schwann cells. We generated BIG1 conditional knockout mice that would be suitable for the Cre-loxP recombination system (Fig. 1A). Schwann cell-specific Dhh promoter-driven Cre recombinase transgenic mice were used for the deletion of loxP-flanked genes (22). Production of BIG1 conditional knockout (tentatively referred to as BIG1 f l/f l ; Dhh-Cre) mice was confirmed by genomic polymerase chain reaction (PCR) and Southern blotting with specific primers and probes, respectively, using tail genomic DNA (Fig. 1, B and C). Through immunoblotting with the respective specific antibodies in sciatic nerves, BIG1 conditional knockout did not affect expression levels of Arf1 and actin, whereas it decreased MPZ levels (Fig. 1, D and E). The expression levels of Sox10 and Oct6 (also known as Scip or POU3F1), which are transcription factors controlling Schwann cell fate (1), were comparable in knockout mice and littermate controls (fig. S1, C and D). In contrast, the levels of Krox20 (also known as Egr2), which is a transcription factor required for triggering Schwann cell myelination (1), were decreased in knockout mice, revealing that BIG1 is involved in the regulation of myelination. As mice developed, the expression levels of Krox20, as well as those of MPZ, became comparable in knockout mice and controls (fig. S2, A and C), suggesting that BIG1 is involved in the regulation of myelination, especially for its initiation.
BIG1 plays a key role in controlling transporting systems in the Golgi network where Arf1 is activated. Arf1 participates in the assembly of AP1 subunit proteins, which are important for anterograde transports from the trans-Golgi (16)(17)(18). Also, Arf1 binds GGA1, which contributes primarily to transports from the trans-Golgi to the lysosome (19). To explore whether knockout of BIG1 has an effect on Arf1 interaction with these components, we prepared tissue lysates from the sciatic nerves of BIG1 conditional knockout (BIG1 f l/f l ; Dhh-Cre) mice and littermate controls. A complex forming AP1 is composed of heterogeneous subunit proteins, including AP1B1, AP1G1, AP1M1, and AP1S1. Thus, we performed immunoprecipitation with an anti-AP1G1 antibody. In control experiments, Arf1 was co-immunoprecipitated with AP1G1. AP1B1, AP1M1, and AP1S1 were also contained in AP1G1 immunoprecipitates. In contrast, in knockout mouse tissue lysates, the amounts of Arf1, as well as those of AP1B1, AP1M1, and AP1S1 in AP1G1 immunoprecipitates, were greatly decreased ( Fig.  1, F and G), indicating the involvement of BIG1 in AP1 formation. Expression levels of Arf1, AP1B1, AP1G1, AP1M1, and AP1S1 were comparable in knockout mice and controls. Similarly, we performed immunoprecipitation with an anti-GGA1 antibody. The amounts of Arf1 in GGA1 immunoprecipitates were comparable in knockout mouse and control tissue lysates ( fig. S3, A and B), and expression levels of Arf1 and GGA1 were also comparable.
In addition, Arf1 is important for the assembly of subunit proteins forming COPI, which participates in retrograde transports from the trans-Golgi to the endoplasmic reticulum (20,21). Immunoprecipitation with an antibody for COPB1, whose subunit constitutes a COPI's first subcomponent group with other subunit proteins COPD, COPG1, and COPZ1, was comparable in knockout mouse and control tissue lysates ( fig. S3, C and D). Expression levels of Arf1, COPB1, COPD, COPG1, and COPZ1 were also comparable. Collectively, BIG1 primarily regulates the AP1 transporting system in Schwann cells.
Because BIG1 conditional knockout mice exhibited decreased MPZ and Krox20 levels, we analyzed the myelin sheath ultrastructure in sciatic nerves. Electron microscopic analysis revealed decreased myelin thickness in sciatic nerves from 7-day-old knockout (BIG1 f l/f l ; Dhh-Cre) mice, compared to those from controls ( Fig. 2A). This decreased myelin thickness in knockout mice is clearly evident from the quantification of the average g-ratio, which is the numerical average ratio of the axon diameter to the diameter of the outermost myelinated fiber. Thinner myelin sheaths with larger average g-ratios were generated in 5-day-old knockout mice (0.94 ± 0.035 in knockout mice, compared to 0.76 ± 0.061 in controls; Fig. 2, B and C). Similar results (Fig. 2D) were observed in 2-month-old knockout mice (0.70 ± 0.049 in knockout mice, compared to 0.63 ± 0.036 in controls; Fig. 2, E and F), revealing that the reduction in myelin thickness persists throughout development in knockout mice. Although the difference of average g-ratios between knockout mice and controls was significant, it became smaller as development proceeded. Therefore, these findings suggest that BIG1 is required for myelination, especially for its initiation. High-magnification electron microscopic analysis indicated that decreased myelin thickness was not due to a difference of thickness between the myelin sheath and the neighboring myelin sheath but was due instead to decreased numbers of wrapping myelin membranes ( fig. S4A).
To confirm that these results are not specific to Dhh promoterdriven Cre recombinase-mediated conditional knockout, we also crossbred other Schwann cell-specific, MPZ promoter-driven Cre recombinase transgenic mice (23) with BIG1 floxed mice and generated conditional knockout (referred to as BIG1 f l/f l ; MPZ-Cre) mice. Consistent with the results from BIG1 f l/f l ; Dhh-Cre mice, thinner myelin sheaths, along with decreased MPZ expression, were also observed in knockout mice (0.83 ± 0.049 in knockout mice, compared to 0.  The loxP-flanked exons, which were digested by Spe I and Sac I, were confirmed with Southern blotting using a specific probe. (C) Genetically modified mice (floxed and Cre mice) were identified with the tail's genomic PCR using the respective, specific primers. (D and E) Sciatic nerve tissue lysates of conditional knockout (BIG1 fl/fl ; Dhh-Cre) mice and their littermate controls were used for immunoblotting with the respective antibodies for BIG1, Arf1, Arf6, MPZ, and control actin. MPZ expression levels are statistically shown (*P < 0.01; n = 3 blots). (F and G) Tissue lysates were used for immunoblotting with the respective antibodies for Arf1, AP1B1, AP1G1, AP1M1, and AP1S1, following immunoprecipitation with an anti-AP1G1 antibody. Total proteins and Arf1 co-immunoprecipitation's statistical data (*P < 0.01; n = 3 blots) are also shown. Arf1 regulates the initiation of myelination in the peripheral nervous system Next, we asked whether Arf1 is involved in the regulation of myelination by Schwann cells. We generated Arf1 conditional knockout (tentatively referred to as Arf1 f l/f l ; Dhh-Cre) mice (Fig. 3A). The mice were confirmed by genomic PCR and Southern blotting (Fig. 3, B and C). Although Arf1 conditional knockout mice did not affect expression levels of BIG1 and actin, they did exhibit decreased MPZ levels (Fig. 3, D and E). The expression levels of Sox10 and Oct6 were comparable in knockout mice and controls (fig. S1, E and F); in contrast, those of Krox20 were decreased in knockout mice, revealing that Arf1 is involved in the regulation of myelination. As mice developed, the expression levels of Krox20, as well as those of MPZ, became comparable in knockout mice and controls ( fig. S2, B and D), suggesting that Arf1 is involved in the regulation of myelination, especially for its initiation.
In electron microscopic analysis, 7-day-old knockout (Arf1 f l/f l ; Dhh-Cre) mice exhibited decreased myelin thickness, compared to those from controls (Fig. 4A). This decreased myelin thickness in conditional knockout mice is clearly evident from quantification of the average g-ratio (0.82 ± 0.060 in knockout mice, compared to 0.72 ± 0.036 in the controls; Fig. 4, B and C). Similar results (Fig. 4D) were observed in 2-month-old knockout mice (0.68 ± 0.047 in knockout mice, compared to 0.65 ± 0.033 in controls; Fig. 4, E and F). These results reveal the reduction in myelin thickness throughout development in knockout mice, although the difference of average g-ratios between knockout mice and controls decreased as development proceeded. It is suggested that Arf1 is required for the initiation of myelination. High-magnification electron microscopic analysis indicated that the thickness between the myelin sheath and the neighboring myelin sheath was comparable in knockout mice and controls ( fig. S4B).
We also crossbred MPZ promoter-driven Cre recombinase transgenic mice with Arf1 floxed mice to generate conditional knockout (referred to as Arf1 f l/f l ; MPZ-Cre) mice. Consistent with the results from Arf1 f l/f l ; Dhh-Cre mice, thinner myelin sheaths, along with decreased   Arf1 knockout does not affect cell proliferation or apoptosis We stained 7-day-old Arf1 conditional knockout mouse (Arf1 f l/f l ; Dhh-Cre) sciatic nerves with antibodies for Ki67 and active, cleaved caspase-3. Staining for Ki67 ( fig. S9, A and B) and cleaved caspase-3 ( fig. S10, A and B) indicated comparable results in knockout mice and controls. Similar results were obtained from immunoblotting in developing sciatic nerves (figs. S9C and S10C).
Knockout of BIG1 or Arf1 decreases MPZ distribution in growing myelin sheaths Since BIG1 conditional knockout mice exhibited decreased amounts of Arf1 in active AP1 subunits, with a thin myelin thickness phenotype, we asked whether knockout of BIG1 or Arf1 is actually associated with a defect of the intracellular transporting system. Myelin components are transported from cell bodies of Schwann cells via various systems, one of which may be the AP1 transporting system (24)(25)(26). Multiple myelin layers are formed by incorporating newly generated, growing myelin membranes underneath previously completed membranes (27). Therefore, if there is a defect of the transporting system in the case of either protein or mRNA level, there are relatively smaller amounts of the myelin component in the inner myelin sheaths. Thus, we tried to detect an MPZ antigen of myelin sheaths by immune electron microscopy (28). MPZ is a myelin component, and it contributes to the interaction of the myelin sheath with the neighboring myelin sheath. BIG1 knockout mice (BIG1 f l/f l ; Dhh-Cre) showed a decrease in the relative amounts of MPZ in inner myelin sheaths (Fig. 5, A and B). Similar results are obtained from Arf1 knockout mice (Arf1 f l/f l ; Dhh-Cre;

DISCUSSION
In the peripheral nervous system, myelin sheaths result in the Schwann cell plasma membranes forming multiple layers. The total area of the sheaths often grows to more than 100 times larger than that of the plasma membranes of premyelinating Schwann cells. Thus, myelination processes undergo continuous and dynamic morphological changes, which involve not only cytoskeletal rearrangements but also various types of transport (3)(4)(5). These two cellular events are generally linked to cell morphological changes and, in turn, tissue morphogenesis in many types of cells and tissues where Arf proteins contribute, at least in part, to their morphogenesis (6)(7)(8)(9). The question of whether the prototypic Arf family protein Arf1 actually regulates formation of multiple myelin sheaths remains unanswered. Here, we show that Arf1 GEF, BIG1, and the effector Arf1 are required for the initiation of myelination in the peripheral nervous system. The conclusions are obtained from the data using generated BIG1 and Arf1 conditional knockout mice. Compared to littermate controls, decreased myelin thickness is observed in Schwann cell-specific BIG1 conditional knockout mice, especially in the initiation of myelination. The amounts of Arf1 in AP1 subunits are greatly decreased in knockout mice. In contrast, the amounts of Arf1 in COPI subunits and Arf1 binding to GGA1 are comparable in BIG1 knockout mice and controls, revealing that BIG1 primarily regulates the AP1 transporting system in Schwann cells. Decreased myelin thickness is also observed in Schwann cell-specific Arf1 conditional knockout mice. Thus, BIG1 and Arf1 contribute to the initiation of myelination in the peripheral nervous system. This proposal is further supported by the results showing that expression levels of the myelination-related transcription factor Krox20, as well as those of the major myelin membrane protein MPZ, become comparable in BIG1 or Arf1 knockout mice and controls as mice develop. Furthermore, since it is unlikely that BIG1 and Arf1 are directly involved in the regulation of the transcription factor (7-9), they may mediate Krox20 expression through activities of signaling molecules directly controlling expression of the transcription factor in early developmental stages. Further studies will allow us to clarify whether or how BIG1 and Arf1, acting together with identified or unidentified interacting molecules, promote myelination, probably by mediating the transportation of myelin components and the expression of identified and unidentified myelinationrelated transcription factors.
Thus far, genomes of human and rodents as the model encodẽ 15 GEFs for Arf proteins, whose common feature is to contain catalytic Sec7 domains (6-9). Among them, GEFs harboring catalytic activity for Arf6 include more than 10 molecular species. We previously reported the involvement of cytohesin subfamily GEFs as the Arf6 GEF involved in myelination by Schwann cells (12,29,30). When myelination is initiated, cytohesin-1 is expressed at high levels. Cytohesin-1 probably supports the activity of Arf6 in Schwann cells in initiating myelination. Following development, cytohesin-2, rather than cytohesin-1, is up-regulated to stimulate the Arf6 activity. It is likely that cytohein-2 is required for sustaining myelination. Despite the participation of Arf6 GEFs and cytohesin-1 and cytohesin-2 in myelination, it remains unclear whether a GEF for Arf1 is involved in the regulation of myelination. GEFs for Arf1 are composed of two distinct families: BIG1 and its homologs and Golgi brefeldin A resistance factor 1 (GBF1) (17,18,20,21). BIG1 generally functions as the GEF of Arf1 on the trans-Golgi and mediates anterograde transports in the trans-Golgi network where an AP1 complex functions. On the other hand, GBF1 functions as the Arf1 GEF on the cis-Golgi to control retrograde transports in the direction from the Golgi apparatus to the endoplasmic reticulum where a COPI complex functions. Myelin formation in early, postnatal developmental stages requires very active transport throughout the trans-Golgi network to the peripheral regions of the myelin membrane (25). Furthermore, it is possible that BIG2, a molecule closely related to BIG1, regulates myelination. Transcriptional products of BIG1 are broadly detected throughout various tissues including nerve tissues, whereas those of BIG2 are detected at a relatively low level (see the UniGene website, www.ncbi.nlm.nih.gov/unigene/). Additional studies on the distribution of BIG1 and BIG2 transcriptional products will shed light on their roles, although BIG1 is involved in initiating myelination in the peripheral nervous system. Acting with other GEF(s) for Arf1 and Arf proteins, BIG1 may play key roles in myelination.
Although the molecular mechanism by which GEFs are activated is complicated and varied depending on the GEF subfamilies involved, phosphorylation events of GEFs themselves are well recognized as one of the major regulatory factors. The phosphorylating mechanism involved in the activation of Vav, which is the GEF specific for the Rho family GTPase RhoA, has been previously identified (31). Vav has an autoinhibitory domain for catalytic domain, and the region is released upon Vav's tyrosine phosphorylation. The catalytic domain of Vav results in the interaction with RhoA to be activated. A similar mechanism is also conserved in the case of the activation of cytohesin-1 (12). Tyrosine phosphorylation of an autoinhibitory region in cytohesin-1 releases its region from the catalytic domain. In turn, Arf6 interacts with the catalytic domain of cytohesin-1. BIG1 has many potential phosphorylation sites involving tyrosine and serine/threonine kinases (see the NetPhos website, www.cbs.dtu.dk/services/NetPhos/). The activity of BIG1 may be regulated by phosphorylation.
Hereditary peripheral neuropathies are associated with GEFs for Rho family GTPases. The mutation of the arhgef10 (also known as gef10) gene is responsible for a peripheral neuropathy with thinly myelinated axons (32). The gene product is a GEF specific for RhoA. The mutation results in slowed nerve conduction velocities, as seen in Charcot-Marie-Tooth (CMT) diseases. Also, the mutation of the frabin [also called fyve, rho-gef, and pleckstrin homology domain containing 4 ( fgd4)] gene causes CMT disease type 4H (33,34). Frabin is a GEF specific for Cdc42. The mutations of the arhgef10 and frabin are probably associated with improper regulation of their gene products and affect Schwann cell morphological changes. On the other hand, the mutation of the big2 (also known as arfgef2) gene is linked to periventricular heterotopia with microcephaly, which involves a severe developmental defect of the cerebral cortex in the central nervous system (35). Because BIG1, as well as BIG2, plays multiple roles involving cell morphological changes (8,9), it could be noteworthy to investigate the association of the big1 gene with peripheral and central neuropathies.
Here, we identify BIG1 and Arf1 as the regulatory molecules of myelination in the peripheral nervous system. Further studies will enhance our understanding not only of how BIG1 and Arf1 are regulated in a series of myelination processes but also of the detailed mechanism by which they physically and physiologically associate with their upstream or downstream molecules during myelination. Along this line, such studies on the relationship of the BIG1 and Arf1 unit with myelination may provide us with a paradigm of the molecular basis for remyelination and nerve regeneration.
In all experiments, PCR amplification (using LA or EX Taq polymerase; Takara Bio) was performed in 35 cycles, with each cycle consisting of denaturation at 94°C for 1 min, annealing at 60°to 68°C for 1 min, depending on the primer pair's annealing temperature, and extension at 72°C for 1 or 5 min.
When it was possible to determine their sex, male mice were used for experiments. Homozygous mice, as well as heterozygous mice, were fertile under standard breeding conditions. Tissue lysis, immunoprecipitation, and immunoblotting Tissues were lysed in lysis buffer A [50 mM Hepes-NaOH (pH 7.5), 3 mM MgCl 2 , 150 mM NaCl, 1 mM dithiothreitol, 1 mM phenylmethane sulfonylfluoride, leupeptin (1 mg/ml), 1 mM EDTA, 1 mM Na 3 VO 4 , and 10 mM NaF] containing biochemical detergents (0.5% NP-40, 1% CHAPS, and 0.03% SDS). Unless otherwise indicated, all lysis steps were performed at 4°C (12,36). For immunoprecipitations, the supernatants cleared by centrifugation were mixed with an antibodyabsorbed protein G resin (GE Healthcare) or ExtraCruz (Santa Cruz Biotechnology) according to the respective manufacturer's instructions. The immunoprecipitates or proteins in the cell supernatants were denatured, subjected to SDS-polyacrylamide gel electrophoresis, and blotted to polyvinylidene difluoride membranes using the TransBlot TurboTransfer System (Bio-Rad). The membranes were blocked with Blocking One (Nacalai) and immunoblotted using a primary antibody followed by a peroxidase-conjugated secondary antibody. The bound antibodies were detected using Chemiluminescence One (standard and strong detection reagents; Nacalai). The bands were scanned using a C-DiGit Blot Scanner (MS Systems). They were densitometrically analyzed to identify their quantification using UN-SCAN-IT software (Silk Scientific).

Tissue immunofluorescence
Nerve tissues were perfused with phosphate-buffered saline (PBS) and then with PBS containing 4% paraformaldehyde (12,36). They were postfixed with 4% paraformaldehyde, replaced with 20% sucrose, and embedded in a Tissue-Tek reagent (Sakura Finetechnical). Microtome sections (NX70; Thermo Fisher Scientific) were blocked with Blocking One and incubated first with a primary antibody and then with a fluorescent substance-labeled secondary antibody. The glass coverslips were mounted with Vectashield Mounting Medium (Vector Laboratories). The fluorescent images were collected with an IX81 microscope system with a laser-scanning FV500 or FV1000D (Olympus). Fluorescent substance-labeled cells were considered to be those with a value greater than 5000 using UN-SCAN-IT software.
Electron microscopy and immunoelectron microscopy For electron microscopy (12,36), nerve tissues were fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.1% cacodylate buffer, contrasted with 2% osmium tetroxide, dehydrated with an ethanol gradient, and treated with propylene oxide. Finally, samples were infiltrated and embedded in pure epoxy. Ultrathin sections (Ultracut UCT; Leica) were stained with uranyl acetate and lead staining solution.
For immunoelectron microscopy (28), tissues were fixed with 4% paraformaldehyde and 0.1% glutaraldehyde in PBS, dehydrated with an ethanol gradient, and infiltrated with a 1:1 mixture of ethanol and resin (LR white; London Resin). The samples were transferred to 100% resin and polymerized by an ultraviolet polymerizer. Ultrathin sections were mounted with nickel grids. Grids were blocked, incubated first with a primary antibody and then with a 10-nm-diameter gold particleconjugated secondary antibody. They were then stained with uranyl acetate and lead staining solution. Images were taken with a JEM-1200EX or JEM-1400 Plus electron microscope system (JEOL). The g-ratio is the ratio of axon diameters to the outer myelinated axon diameters. These ratios were plotted for axon diameter. Their distributions were also shown in the graph.

Statistical analysis
Values are means ± SD from separate experiments. Intergroup comparisons were performed by Student's t test using Microsoft Excel. A oneway analysis of variance (ANOVA) was followed by a Fisher's protected least significant difference test as a post hoc comparison using AnalystSoft StatPlus. Differences were considered significant when P < 0.05.

Ethics statement
Genetically modified/unmodified rodents were generated and maintained in accordance with a protocol approved both by the Tokyo University of Pharmacy and Life Sciences Animal Care Committee and by the Japanese National Research Institute for Child Health and Development Animal Care Committee.

SUPPLEMENTARY MATERIALS
Supplementary material for this article is available at http://advances.sciencemag.org/cgi/ content/full/4/4/eaar4471/DC1 fig. S1. Changes in expression levels of BIG1, the related proteins, and myelin marker protein MPZ during sciatic nerve development and the effect of BIG1 or Arf1 knockout on myelination-related transcription factor Krox20 expression. fig. S2. Effects of knockout of BIG1 or Arf1 on Krox20 or MPZ expression during development. fig. S3. Effects of knockout of BIG1 on the amounts of Arf1 in transporting protein complexes. fig. S4. Comparison of thickness between the myelin sheath and the neighboring myelin sheath in Dhh-Cre-mediated BIG1 or Arf1 knockout mice and controls. fig. S5. MPZ-Cre-mediated BIG1 knockout mice exhibit decreased myelin thickness. fig. S6. Staining for the proliferating cell marker (Ki67) in Dhh-Cre-mediated BIG1 knockout and control mouse nerve cross sections and Ki67 expression during development. fig. S7. Staining for the apoptotic cell marker (cleaved caspase-3) in Dhh-Cre-mediated BIG1 knockout and control mouse nerve cross sections and caspase-3 expression during development. fig. S8. MPZ-Cre-mediated Arf1 knockout mice exhibit decreased myelin thickness. fig. S9. Staining for the proliferating cell marker (Ki67) in Dhh-Cre-mediated Arf1 knockout and control mouse nerve cross sections and Ki67 expression during development. fig. S10. Staining for the apoptotic cell marker (cleaved caspase-3) in Dhh-Cre-mediated Arf1 knockout and control mouse nerve cross sections and caspase-3 expression during development.