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Shape Change and the Percentage of Sialic Acid Removed by Neuraminidase from Human Platelets 1

Summary The sialic acid of human gelfiltered platelets was studied before and after ADP-induced shape change. Neuraminidase cleaved 47% of the total sialic acid from both discoid control platelets and platelets that had become spiny spheres after treatment with 5 μM ADP. We are grateful to the following colleagues for materials used in these experiments: Thomas H. Finlay, Ph.D., for the radiolabeled TAMe, Joel U. Harris, Ph.D., for the radiolabeled fibrinogen, Henriette Lackner, M. D., for the connective tissue suspension, and John Fenton, II, Ph.D. for the purified human thrombin.