{'fraction_non_alphanumeric': 0.019374068554396422, 'fraction_numerical': 0.010432190760059613, 'mean_word_length': 4.843478260869565, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
A microassay for studying allergic histamine release from human leukocytes using an enzymic-isotopic assay for histamine.

Abstract An in vitro microassay is described for studying allergic release of histamine from human leukocytes. The procedure takes advantage of a very sensitive enzymicisotopic assay for histamine which measures the histamine in a 20 μl sample volume; the assay can detect 0.1 ng. histamine. Each leukocyte reaction is run in a total volume of 0.1 ml. An antigen dose-response curve requires only 1 ml. of donor blood. This method is particularly advantageous for studies with children and when repetitive bleeding of individual donors is required.