{'fraction_non_alphanumeric': 0.043507817811012914, 'fraction_numerical': 0.026512576478585997, 'mean_word_length': 5.082644628099174, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Isolation and Structure of a Peptide Hormone Predicted from a mRNA Sequence A THE CATFISH

This laboratory recently observed a cDNA reverse transcript to a specific mRNA from the channel catfish (Ictalurus punctata) pancreas which predicted the presence of a somatostatin gene product different from the previously reported catfish somatostatin (Oyama, H., Bradshaw, R. A., Bates, 0. J., and Permutt, A. (1980) J. BioL C h m . 255, 2251-2254). The gene product was purified using reverse phase high performance liquid chromatography. The peptide was shown to be structurally identical with somatostatin-14 from other spe- cies by tryptic mapping, amino acid composition, and sequence. The level of somatostatin-14 in the catfish endocrine pancreas was determined to be 104 pg of immunoreactive somatostatin/g of tissue. These obser-vations suggest that the catfish pancreas has at least two structurally distinct somatostatins. The structure of somatostatin appears to be highly con-served in most organisms. The sequences of porcine hypothalamic somatostatin 1-14, (l), pigeon pancreatic SST-14' (2), rat pancreatic SST-14 (3), and angler fiih pancreatic SST-14 (4) have been shown to be identical with ovine hypothalamic SST-14 (5). The great similarity of somatostatin structure from disparate sources has one apparent exception. Somatostatin isolated from channel catfish (Ictaluruspunctata) pancreas differs greatly from somatostatin isolated from other sources (6).