{'fraction_non_alphanumeric': 0.05042016806722689, 'fraction_numerical': 0.0392156862745098, 'mean_word_length': 4.742765273311897, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
METHYLENETETRAHYDROFOLATE REDUCTASE POLYMORPHISMS IN PREECLAMPSIA AND THE HELLP SYNDROME

Objective: To investigate the prevalence of the 677 (C → T) and 1298 (A → C) polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene in our preeclamptic population. For a summary estimation of the risk of the 677 (C → T) polymorphism for preeclampsia, we also performed a meta-analysis on four previously published case-control studies to which our results were added. Methods: Genotypes were analyzed by polymerase chain reaction followed by restriction enzyme analysis. The results of 176 nonpregnant women, previously hospitalized for preeclampsia in a tertiary care center, were compared with 403 Dutch population-based controls.Results were statistically analyzed with a chi-square test. Mean Outcome Measures: The incidence of the 677 (C → T) and 1298 (A → C) polymorphisms in the MTHFR gene. Results: The incidence of both MTHFR missense polymorphisms was not significantly different between cases and controls. We found an odds ratio (OR) of 1.5 [95% confidence interval (CI) 0.8–0.6, p = 0.17] and an OR of 1.0 (95% CI 0.6–1.9, p = 0.23) for the 677 (C → T) and the 1298 (A → C) polymorphism, respectively, in cases comparing the prevalence of the homozygous genotype versus the other two genotypes. The meta-analysis resulted in a significant OR of 2.0 (95% CI 1.4–2.9). Conclusions: In contrast to four previous studies, we were neither able to confirm an increased risk for preeclampsia to the 677 (C → T) polymorphism nor did we find an increased risk for preeclampsia to the 1298 (A → C) polymorphism. From the meta-analysis, however, we conclude that it cannot be ruled out that the homozygous 677TT genotype is a modest but significant risk factor for preeclampsia.