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[Detection of tetrodotoxin-sensitive structures in membrane and cytoplasmic fractions of Paralitodes camtschatica King crab nerve].

The membrane fraction isolated from the crab nerve was treated by ultrasonic irradiation to obtain closed osmotically active vesicles. The liposomes from brain lipids were modified by a cytoplasmic fraction to assay the tetrodotoxin influence on the 22Na influx into such liposomes. The 22Na influx into vesicles and modified liposomes was studied in the presence and absence of substances which change sodium conductance of excitable membranes. It is shown that tetrodotoxin at concentrations blocking electrical excitation in vivo decreased the sodium influx into membrane vesicles and modified liposomes.