{'fraction_non_alphanumeric': 0.04193849021435228, 'fraction_numerical': 0.0521901211556384, 'mean_word_length': 4.451776649746193, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Comparison of Gene Expression Effects in Liver Tissue and Primary Hepatocyte Cell Cultures After Exposure to Hexahydro-1, 3, 5Trinitro-1, 3, 5-Triazine

We examined Sprague-Dawley rat liver tissue and primary hepatocyte cells to understand how they compared in gene expression responses to hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). Gene expression was analyzed in primary cell cultures exposed to 7.5, 15, or 30 mg/L RDX for 24 and 48 hours. Cells were compared to liver from rats 24 hours after exposure to 12, 24, or 48 mg/Kg RDX. Similarity of present/absent calls to liver in control exposures was 88% at 24 hrs and 79% at 48 hrs. Significant changes were identified using mixed-model analysis of variance. Up to 6.4% of differentially expressed genes present in liver were differentially expressed in 24 hr cells and less than 5.5% in 48 hr cells. Of genes with identifiable KEGG pathways, 24 hr cells 78.4% similar to liver pathways and 48 hr cells were 44.3% similar. These data suggest care should be taken extrapolating effects from in vitro to in vivo levels