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Isolation and Biochemical characterization of thermophilic Streptomyces from soil of Raipur District of Chhattisgarh, India

The present study was aimed to isolate and characterize the thermophilic Streptomyces from soil of Raipur district of Chhattisgarh. The samples were collected from 16 blocks of Raipur district, mainly from the compost and garbage soil. Isolation of thermophilic Streptomyces was carried out from the samples using serial dilution spread plate method with starch casein agar medium. Temperature was maintained at 45 o C for isolation of thermophilic Streptomyces. Total 42 thermophilic Streptomyces were isolated and characterized on the basis of colour of spore mass, reverse side colour, aerial and substrate mycelia formation and production of diffusible pigments. Out of 42 isolates, 20 were selected on the basis of temperature optima for further studies. The selected isolates were further characterized on the basis of biochemical tests and antimicrobial activity against eight microbial strains i.e. Escherichia coli, Stapylococus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Aspergillus niger, Penicillium notatum, Penicillium chrysogenum and Candida albicans. The growth of different Streptomyces isolates were recorded in presence of different carbon and nitrogen sources, different concentrations of NaCl and at different pH. The growth of different isolates of Streptomyces was also observed in presence of different chemical inhibitors viz. potassium tellurite, sodium azide, phenol and crystal violet. All isolates were identified using PIB Win (Probabilistic identification of bacteria) software.