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[The development and collagen ingredients analysis of submandibular gland acellular matrix].

PURPOSE
To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients.


METHODS
The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry.


RESULTS
Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy. I, II and III type collagen proteins of submandibular gland before and after cell-extracted were positive under immunohistochemistry.


CONCLUSIONS
The main ingredients of submandibular gland acellular matrix were I, II and III type collagen proteins.