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Distribution of GFAP immunoreactive structures in the rhombencephalon of the sterlet (Acipenser ruthenus) and its evolutionary implication.

Previous studies have revealed that although the brains of cypriniform teleosts (iberian barb, Barbus comiza; carp, Cyprinus carpio; goldfish, Carassius auratus) are rich in glial fibrillary acidic protein (GFAP), they have, however, areas devoid of GFAP immunoreactivity. The largest ones of these are in the rhombencephalon, e.g., the zones of the sensory and motor neurons in the vagal lobe. Our studies in amniotes suggested that the GFAP immunonegative areas could be characteristic of the more advanced brains (avian and mammalian), whereas no similar areas were found in reptiles. A similar tendency was found in the Chondrichthyes, i.e., GFAP immunonegative areas appeared as brain complexity progressed. The question arose whether the GFAP immunonegative brain areas in the Teleostei were also the result of such a tendency. Within the radiation of ray-finned fishes (Actinopterygii), Chondrostei represent a less advanced level as compared to the Teleostei. Therefore, the distribution of GFAP immunoreactivity was investigated in the rhombencephalon of the sterlet (Acipenser ruthenus) as a representative of Chondrostei, and in the carp. Serial vibratome sections were processed according to the avidin-biotinylated horseradish peroxidase method.Several comparable GFAP immunoreactive structures were found in the two species: the dense periventricular ependymoglial plexus, the midsagittal glial septum, the small glial septa separating the nerve fiber bundles, and the wide glial endfeet lining the meningeal surface. In the vagal lobe in the zones adjacent to the meningeal and ventricular surfaces, the glial structures also proved to be similar. In contrast to the carp, however, no areas were found devoid of GFAP immunoreactivity in the sterlet.The results suggest that this trend of glial evolution, i.e., GFAP immunonegative areas appearing as brain complexity progressed, is a common feature shared by Actinopterygii, Amniota, and Chondrichthyes, despite their separate evolutionary histories. J. Exp. Zool. 293:395-406, 2002.