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KINETIC CHARACTERIZATION OF THE REDOX COMPONENTS IN SOLUBILIZED MEMBRANES FROM PORCINE NEUTROPHILS: REDUCTION WITH DITHIONITE AND PHOTOEXCITED NAD(P)H

Abstract— Cytochrome b558 in solubilized membranes prepared from porcine neutrophils was reduced by dithionite with a second‐order rate constant of 2.5 times 106 M‐1 s‐1 at pH 7.4 and 20°C accompanied by spectral changes with peaks at 428 nm and 560 nm and isosbestic points at 420 and 441 nm. When an anaerobic mixture of solubilized membranes and NAD(P)H was exposed to a white light, cytochrome b558 was reduced biphasically but with almost the same spectral profiles as in the dithionite reduction. Thus, participation of redox component(s) of unknown nature in the photochemical reduction was suggested.