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Levels of piribedil and its active metabolite (S-584) in the rat striatum.

1. A gas chromatographic-mass fragmentographic method has been developed to determine piribedil and its metabolite S-584, using diazepam as internal standard. The metabolite was derivatized as a trimethylsilyl ether for gas chromatographic analysis. The minimum detectable amount of piribedil and the metabolite was 10 ng/g tissue. 2. Levels of piribedil and its metabolite in the striatum were determined by mass fragmentography.