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DIFFERENTIAL SUSCEPTIBILITY OF SPLEEN CELLS FROM C3H/HeN (H‐2k) AND C3HfB/HeN (H‐2kv1) MOUSE STRAINS TO CYTOTOXICITY BY MONOCLONAL ANTI‐Kk ANTIBODY (CLONE 11‐4)

Spleen cells from C3HfB/HeN mice (H‐2kv1) were markedly less susceptible to lysis by monoclonal anti‐Kk antibody (Clone 11‐4) than spleen cells from C3H/HeN mice (H‐2k). Less than 50% of C3HfB/HeN cells were lysed even at the highest antibody concentration tested compared to 70% of C3H/HeN cells. The difference in end point titre of the antibody tested on cells from the two strains was 5‐10‐fold. Similar differences were not observed using the conventional H‐2Kk‐typing antiserum (D‐23).