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Comparative evaluation of Brassica oleracea, Ocimum basilicum, and Moringa oleifera leaf extracts on lipase inhibition and adipogenesis in 3T3-L1 adipocytes.

The anti-adipogenic effects of alcohol extract of Brassica oleracea (BO), Ocimum basilicum (OB), and Moringa oleifera (MO) leaves on 3T3-L1 pre-adipocyte as well as their lipase inhibitory properties were comparatively evaluated. The polyphenol-rich extracts of MO, BO, and OB leaves were profiled for major bioactive compounds using UPLC-HRMS/MS analysis. Among the three plant extracts, BO had the highest flavonoid content with stronger radical scavenging activity. All extracts exhibited lipase inhibitory activities, dose-dependently and a non-competitive type of inhibition with altered kinetic parameters was observed. In cell culture studies, these plant extracts remarkably inhibited the intracellular triglyceride accumulation in 3T3-L1 cells and the effect was in the decreasing order of BO > OB > MO. The extracts also down-regulated the key transcription factors of adipogenesis (PPARγ, C/EPB-α, PI3k, p-Akt, and FAS enzyme) as well as modulated the release profile of leptin and adiponectins. Overall, BO extract showed an exceptional inhibitory potential against both adipogenesis and lipase activities among the evaluated plants. PRACTICAL APPLICATIONS: The present study highlights the anti-adipogenic potential of three local edible plants and herbs, that is, Brassica oleracea (BO), Ocimum basilicum (OB), and Moringa oleifera (MO) leaves, using in vitro models. The results revealed that the BO extract had remarkable activity against adipogenesis in the 3T3-L1 pre-adipocyte differentiation model as well as lipase inhibitory properties. This study elucidates the prospects of natural plant-derived compounds in managing obesity-related health issues. The outcomes of this study would be useful in developing alternative or complementary therapies that are being preferred and largely sought over pharmacological treatments and procedures for controlling and treating abnormal weight gain in humans.