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Study of nucleic acids interactions with platinum based cytostatics using biosensor

As a consequence of the employment of platinum based cytostatic drugs in tumour diseases treatment, it became necessary not only to detect them in biological samples but also to determine and study the Pt‐DNA adducts. The main of this work was to study the interactions between DNA and platinum based cytostatics (such as cisplatin and carboplatin). Primarily, we optimized the electrochemical detection of the drugs with detection limit of cisplatin 100 pM. The current accepted opinion about platinum based cytostatics mechanism of action is that the drugs induce their cytotoxic properties through binding to the nuclear DNA. Thus we followed by investigation of DNA interactions with the drugs. Due to this we have suggested a new biosensor, EDTA MT biosensor, and used cyclic voltammetry as a comparative technique. The average concentration of the drug bound to DNA was estimated as 8.1 ng of cisplatin per 500 ng of DNA by EDTA MT biosensor. It follows from the results obtained that the suggested biosensor could be a new useful tool for investigation of interaction of DNA with the drugs, because give a response only in the present of Pt(II)‐DNA adduct.