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[Effect of metanephric mesenchyme cells on podocytes apoptosis induced by high glucose].

OBJECTIVE
To study the effect of metanephric mesenchyme cells on podocytes apoptosis induced by high glucose.


METHODS
Mice's podocyte was cultured in vitro,and apoptosis and injury model of podocyte was then established by high glucose (30 mmol/L) induction. Metanephric mesenchyme cells were extracted from E13.5 mouse embryos and used to make conditioned medium which was used to treat podocytes apoptosis. The flow cytometry and confocal fluorescence imaging were used to detect the apoptosis ratio and cytoskeletal protein (synaptopodin) expression of podocyte at several time points (24, 48, 72 h), in order to explore the effect of high glucose on podocytes and the treatment effect of metanephric mesenchyme cell conditioned medium.


RESULTS
Significant increasing of podocyte apoptosis ratio and decreasing in synaptopodin expression contrast to control group was observed after induction of high glucose, with a statistical difference. Metanephric mesenchyme cells could be isolated from E13.5 mouse embryos successfully, and had the capacity of osteogenic and adipogenic differentiation. Metanephric mesenchyme cell conditioned medium and high glucose stimulations was used to generate podocytes cells. Compared to the group treated with high glucose stimulation, the flow cytometry detection result suggested that metanephric mesenchyme cell could reduce podocytes apoptosis in a dose-dependent manner: with increasing in the concentration of metanephric mesenchyme cell conditioned medium, the treatment effect was better.


CONCLUSION
Metanephric mesenchyme cells could prevent apoptosis and injury of podocyte induced by high glucose.