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Whole-genome Sequencing of Vibrio sinaloensis T47, a Tropical Marine Isolate with Quorum Sensing Properties

A large number of Vibrio sp. thrive in the marine environment and they are notable to cause food borne infection associated with undercooked seafood. In this study, we report the whole genome sequence of Vibrio sinaloensis T47 which was isolated from coastal marine water in Morib Beach, Hulu Selangor. The genome is made up of approximately 4.59 Mbp with 80 contigs and 46% G+C content. From the annotated genome, genes associated with quorum sensing (QS) were identified. This research provides a genetic basis for better understanding of QS pathway which contributes to the physiological traits of strain T47 to thrive in the marine environment.


Introduction
Vibrio sp. is a very common bacterium which can be found in almost all water-borne environments including sea, estuary and fresh water. Vibrio sp. first made its debut into the scientific world through the discovery of bioluminescence properties. It was reported that Vibrio fischeri forms a symbiotic relationship with its host, the Hawaiian bobtail squid (Euprymna scolopes) and this underlying symbiosis is associated with quorum sensing (QS) [1]. QS is well known as a mechanism of virulence and colonization when the population in bacteria surpasses a threshold. This feature seems to be a common trait in the members of the genus Vibrio.
Since the development of advance taxonomical tools such as DNA-DNA hybridization, fluorescent amplified fragment length polymorphism and multilocus sequence analysis, the number of novel species from Vibrio family being discovered is constantly expanding [2,3]. Among the vast members of Vibrio genus, a number of them were demonstrated to possess QS abilities such as V. harveyi [4], V. cholera [5], and V. anguillarum [6]. There is mounting data suggesting that QS is responsible for many unique traits such as pathogenicity, swarming abilities and biofilm production [7,8,9]. In this work, we study on V. sinaloensis strain T47 which was isolated from a tropical marine in Morib Beach, Selangor (2ᵒ 45' 2.7" N, 101ᵒ 26' 34.7" E). A water sample was collected approximately 15 cm from the water surface.
V. sinaloensis was first documented by Gomez-Gil and colleagues [10] from the spotted rose snapper (Lutjanus guttatus) which causes infection and vibriosis. In fact, this bacterium is a major threat to the aquaculture sector due to its pathogenicity properties.

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The colonization of V. sinaloensis has been reported in crustaceans, for example, the white-leg shrimp, Litopenaeus vannamei [11]. Here, the sequencing strategy and data of the whole genome of strain T47 is presented to provide better understanding of the marine bacterium as well as insights to the physiological behaviors associated to QS activity.
V. sinaloensis strainT47 was cultured in aseptic condition on Luria Bertani Agar (LBA) with 3% NaCl concentration (w/v) and incubated at 28°C overnight. The genomic DNA of strain T47 was extracted using QIAamp DNA Minikit (Qiagen, Germany) according to the manufacturer's instructions. The quality of the extracted DNA was measured using NanoDrop Spectrophotometer (Thermo Scientific) and Qubit 2.0 fluorometer (Life Technologies). Next, Nextera DNA Prep Kit (Illumina Inc., CA) was used to prepare the sequencing library followed by whole genome sequencing using a personal sequencer, Illumina MiSeq (Illumina Inc., CA). The total reads were assembled into 80 contigs with 43.8 × coverage using CLC Genomic Workbench version 5.1 (CLC Bio, Denmark). The draft genome of strain T47 is made up of 4,599,504 bp with G+C content of 46.12%. The genome sequence has been deposited into GenBank under the accession number JXBJ00000000. The 16S rDNA sequence used in identification of strain T47 [12] was also deposited into NCBI under accession number KR058860.
Based on 16S rDNA sequence, strain T47 was found to have more than 99% similarity to several Vibrio sp. such as V. variabilis, V. caribbeanicus, and V. sinaloensis. On the other hand, annotations of both functional and predicted genes were performed using the Integrated Microbial Genomes (IMG-ER) platform and with GOLD-ID Ga0063884 [13]. As shown in Table 1, the genome was resolved into 4,105 protein coding genes (CDs) and a total of 127 RNA genes which consist of 8 genes responsible for 5S rRNA synthesis, 5 genes for 16S rRNA synthesis, 6 genes for 23S rRNA synthesis and 107 genes for tRNA. From the IMG-ER platform, cluster of orthologous groups (COG) categories showed that a large number of genes are responsible for basic life-sustaining needs of the bacterium. It was found that 310 genes were predicted to contribute to amino acid transport and metabolism, 245 genes are linked to carbohydrate transport and metabolism, 295 involves in signal transduction mechanisms, and 91 genes are related to the virulence and defense regulation ( Table 2).
From the annotated genome sequences, a gene associated with QS was found in contig 14. The 1203 bp of luxM homologue is analogous to an N-acyl homoserine lactone (AHL) synthase, AinS, which can be also found in V. fischeri [14,15]. Hence, it is highly postulated that the autoinducer synthase LuxM is responsible for the production of signaling molecules in strain T47. In this study, the availability of the sequence could contribute to a better understanding of QS system and its role in V. sinaloensis.

Nucleotide sequence accession numbers
The draft genome sequence of V. sinaloensis strain T47 can be obtained from GenBank under the accession number JXBJ00000000. This version described in the paper is the first version, JXBJ00000000. The GenBank accession number for 16S rDNA nucleotide sequence for strain T47 is KR058860. This version described in the paper is the first version, KR058860.