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Application of X‐ray element mapping across the lichen–rock interface

The application of element mapping in the scanning electron microscope with electron probe microanalysis across the lichen–rock interface is described with reference to Trapelia involuta growing on a granite substratum. The preparation of samples containing both organic and mineral components required the development of specialized techniques to maintain both chemical and structural integrity at the 2 μm resolution of the X‐ray element maps. X‐ray element maps show the distribution of entrained rock particles at the lichen–rock interface and chemical localization which is strongly related to anatomical structure for the essential elements S, Fe, Ca, Na, K and P. The ability to map element distribution across the lichen–rock interface has wide‐ranging potential applications in studies such as the biodeterioration of buildings and monuments and the mobilization and uptake of toxic elements from contaminated substrata.