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Anti-E. coli Maltose Binding Protein Antibodies.

Two hundred microliters prepared solution (50mg of purified recombinant 6XHis-MBP supplemented with 50mg poly [I:C]) and 50 mg of antimouse CD40 monoclonal antibody as adjuvant were injected intraperitoneally into female BALB/c mice (8 weeks of age). The mice were injected twice with a 2-week interval and sera were tested by Western blot. Three days before fusion, the final boost was injected through the tail vein.