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Determination of cytosolic ADP and AMP concentrations and the free energy of ATP hydrolysis in human muscle and brain tissues with 31P NMR spectroscopy

Based on experimentally determined concentrations of PCr, Pi, ATP, and H+, the ADP and AMP concentrations and the free energy for the ATP hydrolysis in normal human brain and muscle were calculated assuming that the creatine kinase‐ and the myokinase‐catalyzed reactions were in equilibrium, accounting for changes in pH and free Mg2+ ion concentration. © 1991 Academic Press, Inc.