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Application of Polymerase Chain Reaction to Determine the Flocculation Properties of Brewer's Lager Yeast

We developed a polymerase chain reaction method to determine the flocculation ability of yeast based on one of the flocculation genes. More than 30 brewer's lager yeast strains were examined, and the amplification of the targeted flocculation gene was confirmed in all of the flocculating yeast strains. The method was also successfully applied to the early detection of nonflocculating mutants by examining yeast cells individually. In some applications, we were able to determine the cause genetically when yeast suddenly did not flocculate and failed to sediment toward the end of fermentation. This method not only yields rapid results but also offers reliability and reproducibility.