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DNA sequence of lipoprotein lipase cDNA cloned from human monocytic leukemia THP-1 cells.

Lipoprotein lipase (LPL) is a key enzyme to catabolize triglyceride-rich lipoproteins at the first step of their metabolism in circulation. A deficiency of LPL activity is known to result in Type I hyperlipoproteinemia with severe fasting chylomicronemia. LPL gene is expressed in a number of tissues including adipose tissue, muscle, placenta, and kidney, but not in liver (1). LPL gene is also expressed in macrophage-like cells (THP-l-MO) derived from human THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA) (2, 3). The cDNA (HLC601) coding for LPL was cloned from THP-1-MO cDNA library in lambda gtlO by hybridization with the synthetic oligonucleotide of human adipose tissue LPL cDNA cloned by Wion et al. (1). HLC601 contained the complete coding region and both DNA strands were sequenced by the Sanger's method (4). The sequence of the coding region for LPL from THP-1-MO was identical to those from adipose tissue (1) and placental source (5). The results indicate that LPL cDNA from THP-1-M4 is a useful tool to study a physiological role of LPL gene expression in human macrophages.