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A radioimmunoassay for digoxin in serum, urine and myocardial tissue.

: A modified radioimmunoassay for digoxin, suitable for serum, urine and homogenized myocardial tissue, is presented. It includes an initial extraction by dichlormethane after addition of protein (gelatine, 4 g/l). A constant fraction and more than 93 per cent of the digoxin is extracted in this way. The method is suitable for pharmacokinetic studies, since the basis for standardization is aqueous solutions of digoxin, and the interference of proteins in the samples are eliminated. By this means the interference of other digitalis glycosides is reduced, and quenching correction under the final determination of the 3H activity is unnecessary. The precision, including the initial extraction, is 5–7 per cent.