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Determination of Vitamin D_3 in Calcium-D granules by HPLC

Objective To establish a HPLC method for determination of Vitamin D 3 in Calcium-D granules. Method A C 18 column was used. The mobile phase consisted of hexane and ethanol(98∶2) and the detection wavelength was 254 nm. Results The linear range was between 12～48 IU·mg -1(r=0.999 7), the average recovery was 99.92%(RSD=0.89%, n=6). Conclusion This method is simple, convenient and is suitable for the quality control of Calcium-D granules.