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Investigating the presence of BPA in infant oral hygiene products using fluorescence spectroscopy

• Bisphenol-A (BPA) is a chemical found in hard plastics. • BPA is an endocrine disruptor that binds to estrogen receptors. This is linked to negative health effects such as cardiovascular diseases and developmental issues. • Infants and young children are more at risk to these effects because their endocrine systems are not fully developed. Materials and Methods: • A variety of children’s toothbrushes, labeled A-H, were selected and tested for the presence of BPA. • Each toothbrush was placed in 100 mL of a 1:1 methanol:water (M/W) solution at room temperature. • A 5-mL aliquot of the solution was taken at time intervals ranging from 5 minutes to 1 week after the toothbrush was placed in the solution. • Each sample was run in the spectrofluorometer in duplicate, where the emission and excitation intensities were reviewed and recorded to determine which toothbrushes contain BPA. Materials and Methods, Continued: • The bristles were removed from the BPA-containing toothbrushes. • The bristles and the head of each toothbrush were then placed separately into 100-mL solutions of 1:1 M/W and analyzed following the protocol described above. • Values were compared to determine the location of BPA in the toothbrushes.