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BIOCHEMICAL CHANGES IN RAT BRAIN ASSOCIATED WITH THE DEVELOPMENT OF THE BLOOD‐BRAIN BARRIER

—Subcellular fractions from brains of 5, 10, 13, 16, 21, 30 day‐old and adult rats were prepared. Protein content and various enzyme activities were assayed in all fractions and brain homogenates. γ‐Glutamyl transpeptidase activity and 5′‐nucleotidase were very low at 5 days of life but steadily increased, reaching adult concentrations at about 30 days after birth. Alkaline phosphatase, instead slowly decreased with maturation, while monoamine oxidase after an initial decrease, increased rapidly to adult levels.