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Molecular analysis of uterine leiomyomas using the probes D7S13(7q) and HSPA2(14q).

Human uterine leiomyomas, mostly cytogenetically characterized, were analyzed by Southern blotting. The region 7q22.3-q31.2 was analyzed in 54 leiomyomas using the polymorphic probe D7S13. The region 14q22-24 was investigated in 25 leiomyomas with the probe HSPA2. No evidence of rearrangement or amplification of these DNA-regions was observed. Nor was any loss of polymorphism for the probe D7S13 seen. These, and earlier negative results, motivate further molecular investigation to understand the mechanisms reflected by recurrent chromosome aberrations in myomas.