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Secretion of mouse α‐amylase from fission yeast Schizosaccharomyces pombe: Presence of chymostatin‐sensitive protease activity in the culture medium

We have constructed two secretion vectors for Schizosaccharomyces pombe using an SV40 promoter and the secretion signals of the pGKL killer toxin complex derived from Kluyveromyces lactis. Although indigenous secretory glycoproteins tend to accumulate in the periplasmic space of S. pombe, we have succeeded in the secretion of mouse α‐amylase into the culture medium. The efficiency of secretion, processing pattern, stability and culture conditions for mouse α‐amylase were studied in S. pombe. The 128 kDa killer secretion signal was more effective in directing secretion of mouse α‐amylase than the 28 kDa killer secretion signal. We detected a chymostatin‐sensitive protease activity in the culture medium of S. pombe, which digests mouse α‐amylase secreted into the culture medium. The addition of 5 μg/ml chymostatin was shown to protect mouse α‐amylases from this degradation.