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Chrysanthemum Cutting Productivity and Rooting Ability Are Improved by Grafting

Chrysanthemum has been commercially propagated by rooting of cuttings, whereas the quality will decline over multiple collections from a single plant. Therefore, we compared the vigour, rooting ability, and some physiological parameters between cuttings harvested from nongrafted “Jinba” (non-grafted cuttings) with those collected from grafted “Jinba” plants onto Artemisia scoparia as a rootstock (grafted cuttings). The yield, length, node number, stem diameter, fresh weight, and dry weight of the grafted cuttings were superior to the non-grafted cuttings. Also grafted cuttings “Jinba” rooted 1 day earlier, but showing enhanced rooting quality including number, length, diameter, and dry weight of roots, where compared to the non-grafted. The physiological parameters that indicated contents of soluble protein, peroxidase activity, soluble sugar, and starch, ratios of soluble sugar/nitrogen ratio, and carbohydrate/nitrogen (C/N), as well as contents of indole-3-acetic acid (IAA) and abscisic acid (ABA), and IAA/ABA ratio were significantly increased in the grafted cuttings. This suggested their important parts in mediating rooting ability. Results from this study showed that grafting improved productivity and rooting ability related to an altered physiology, which provide a means to meet the increasing demand.


Introduction
Vegetative propagation is commonly used to multiply elite individual in a number of horticultural and silvicultural plants [1], and particularly frequently in chrysanthemum (Chrysanthemum grandiflorum). Chrysanthemum is one of the major world ornamental species, and cuttings are normally taken from a stock plant, but their vigour of cuttings tends to decline when the cuttings were harvested repeatedly from the same stock plants. It is laborious to reestablish the stock plants to meet the need of cuttings; the development of techniques to extend the period for cuttings collection would therefore be of significant economic interests.
Unlike nongrafted plants (namely, a stock plant propagated by using cuttings), grafted plants using rootstock of higher vigor or tolerant to abiotic stress have been shown to lead to improvements in economic yield [2], end-use quality [3], water and nutrient uptake and use efficiency [4,5] and abiotic stress tolerance [6][7][8] and increase synthesis of endogenous hormones [9].
Herbaceous species Artemisia scoparia is a useful rootstock to enhance the heat tolerance of chrysanthemum cultivars of "Yidalihong" and "Qiuyi" by decreasing membrane permeability and enhancing activities of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase activity, and soluble protein content [10]. In present study, we compared the productivity and rooting ability of chrysanthemum cuttings obtained from nongrafted (nongrafted cuttings) and grafted cuttings onto Artemisia scoparia (grafted cuttings). In addition, we investigated the content of a number of physiological, metabolic, and hormonal components in these two kinds of cuttings. It aids to improve the yield and quality of cuttings of cut chrysanthemum.

Plant Materials and Growing
Conditions. Chrysanthemum "Jinba" and grafted "Jinba" onto A. scoparia were maintained by the Chrysanthemum Germplasm Resource Preserving Centre, Nanjing Agricultural University, China.  Both nongrafted and grafted stock plants at 6-8 leaf stage were cultured in common cultivation condition. In this cultivation, stock plants of the two type were pinched for only one time, after which three nodes from the base of the stem remained. As such three lateral shoots outgrew on each stock plant. The stock plants for cuttings harvest were fertilized with N : P : K (14.0 : 2.0 : 8.0) every two weeks and watered every two days. The four blocks of rooted cuttings and grafted plants were randomly plotted.

Collection and Productivity of Cuttings.
Three batches of cuttings were collected the harvest times were referred to as H1 (June 17, 2011, 23 days after the initial pinching), H3 (July 27, 63 days), H5 (September 5, 103 days). At each harvest, the basal three nodes of the stock plant's lateral branches were left. Ten randomly selected stock plants per harvest were used for the assessment of biomass production, cutting yield, the length, node number, diameter (diameter of the uppermost node), and the fresh and dry weight of the cuttings.

Determination of Rooting Capacity.
To determine the rooting capacity of the nongrafted and grafted cuttings, a sample of 150 cuttings per harvest (namely, H1, H2, and H5) of 6-8 cm long with three or four nodes was planted in trays filled with a 1 : 1 mixture of perlite and rice chaff ash. During rooting, the material was intermittently treated with mist irrigation during the daylight maintaining a relative humidity >70%. The H5 cuttings were given 3 h of supplementary incandescent lighting (11 p.m. till 2 a.m., 100lx) during the night. Five days after planting cuttings, ten cuttings per treatment were randomly sampled every day to determine the shortest days required for rooting; the day when ∼20% of the cuttings showed visible roots was recorded as rooted [11]. In addition, 15 days after planting the cuttings, root length, diameter, number, and dry matter were measured based on the data collected from 10 randomly sampled cuttings. The test was performed in three replications.

Physiological Parameters.
The basal leaves and stems from cuttings (H1, H3, and H5) were sampled, respectively, for physiological parameters investigation. Protein content was determined following the method of Bradford [12], employing bovine serum albumin as a standard. Peroxidase (POD) activity was assessed as described by Argandoña et al. [13]. The soluble sugar was determined following the methods of van Handel [14] and Paul et al. [15]. The total nitrogen content of the material was obtained using the Kjeldahl method [16], and the carbohydrate/nitrogen (C/N) ratio was obtained by dividing the soluble sugar plus starch by the total nitrogen contents [17]. The indole-3-acetic acid (IAA), abscisic acid (ABA), and gibberellic acid (GA) were quantified by applying protocols [18][19][20], and the hormone standards were purchased from Sigma (St. Louis, MO, USA).

Statistical Analysis.
A one-way analysis of variance (ANOVA) was followed by the application of Duncan's multiple range test ( < 0.05) to assess whether treatment means differed statistically from one another using SPSS v13.0 software package (Chicago, IL, USA).

The Cutting
Productivity. The cutting productivity of the nongrafted and grafted stock chrysanthemums was presented in Table 1. The result showed that the grafted stock plants yielded 42, 150, and 83 cuttings in H1, H3, and H5, respectively, approximately 14%, 53%, and 15% more than did the nongrafted in respective harvest time. As for the cutting quality, that is, the mean length, node number, diameter, and fresh and dry matter of the cuttings, the stock plants performed better in H3 and then in H5 than in H1, irrespective of nongrafted or grafted. Moreover, the quality of the cuttings from grafted stock plants was overall better than that from nongrafted stock plants, except for H1.

Cutting Rooting Capacity.
Rooting capacity of cuttings from nongrafted and grafted stock plants was shown in Table 2. In H1, H3, and H5, the cuttings obtained from grafted stock plants developed roots one day earlier than did those from the nongrafted ones, and the former showed increased maximum length, mean length, mean diameter, mean number, and mean dry matter of roots. This indicated that the grafting improves rooting capacity of Chrysanthemum cuttings. Among the three harvest times herein, The Scientific World Journal 3  the cuttings from grafted stock plants in H3 exhibited better rooting characteristics than the other treatments, other than mean root length and diameter shorter than that in H1.

Biochemical Aspects of Cutting Performance.
Cuttings derived from grafted stock plants accumulated a greater level of soluble protein in their leaves and stems than their equivalents from nongrafted cuttings (Figure 1(a)). The difference was particularly notable in H5 cuttings, where soluble protein in the leaves and stems of grafted cuttings were, respectively, 39.4% and 25.8% higher than that of nongrafted cuttings. POD activity in the leaves and stems of cuttings derived from grafted cuttings tended to be higher than in their equivalents from nongrafted cuttings (Figure 1(b)). In all three samples (H1, H3, and H5), POD activity of stems in grafted cuttings was significantly higher, reaching 99.5% of the cutting seedlings in H1. Similarly, POD activity of leaves in the grafted cuttings was 24.1% higher than nongrafted cutting in H1. There were significant differences between the cuttings obtained from the two kinds of stock plants in soluble sugar and starch content of the leaves and stems (Figures 2(a) and 2(b)). Soluble sugar contents in leaves were higher than those in stems in all three batches. The overall performance of starch content cuttings was similar to the soluble sugar content. The influence of grafting on their total nitrogen content of the cuttings was illustrated in Figure 2(c). Generally, the level of total nitrogen content in grafted cuttings was lower than in nongrafted cuttings, irrespective of harvest time. The sugar/nitrogen and C/N ratios in the leaves and stems of the grafted cuttings were significantly higher than those in nongrafted cuttings (Figures 2(d) and 2(e)).

Phytohormone Content.
The IAA content in the leaves and stems of the grafted cuttings was significantly higher than that in nongrafted cuttings except that in stems harvested in H1 (Figure 3(a)). In contrast, a slight decrease of ABA and GA contents in the grafted cuttings was observed in all treatments (Figures 3(b) and 3(c)). Figure 3(d) showed that IAA/ABA is in leaves of grafted cuttings in all three harvest times, and so observed in stems harvest in H5. In addition, there was no significance in ABA/GA ratio but for a slight increase in stems of grafted cuttings (Figure 3(e)).  The Scientific World Journal

Discussion
The well-established root system enjoyed by grafted plants was associated with an increased potential to generate biomass [21,22]. Here, in chrysanthemum, cuttings taken from grafted stock plants were superior to those taken from nongrafted ones in term of cutting quality and production; also, we found that the grafted cuttings rooted earlier than the nongrafted, moreover, show improved root quality. In previous study we reported the vigorous root system of A. scoparia had a considerable influence on the uptake and translocation of water and nutrients and as such played an essential role in physiological processes of scion such as growth, signal transduction, and development [10]. Therefore, we ascribe the improved productivity and rooting capacity of grafted cutting to the vigorous root system of the rootstock, namely, A. scoparia in this study. Chu et al. [23] had suggested that higher rooting ability had been related to an increase in synthesis of soluble protein during the root regeneration process. In the present study, the leaves and stems of grafted cuttings had higher level of soluble protein content than the nongrafted either (Figure 1(a)), suggesting grafting improving adventitious root formation was partially due to the elevated protein content in grafted cuttings. POD was a useful biochemical marker for analysis of rooting phases for correlation with tissue morphological changes [24]. POD activity in grafted cuttings in this study was higher than that in the nongrafted (Figure 1(b)) and similar to the result in eggplant [25]. Thus grafting might act to promote POD enzyme activities and result in mediating adventitious root development of the cuttings.
The carbohydrate and nitrogen statuses of the plant are known to be important determinants of adventitious root formation [1]. Rouphael et al. [4] noted that when miniwatermelon plants were grafted onto a Cucurbita maxima Duchesne × Cucurbita moschata Duchesne hybrid rootstock grown under open field conditions, the leaf nitrogen content in grafted plant was higher than nongrafted plants. Yang et al. [17] had suggested that the sugar/nitrogen and C/N ratios were also influential on rooting ability. In the present study, the grafted cuttings had higher level of soluble sugar and starch contents, as well as both superior sugar/nitrogen and C/N ratios ( Figure 2). As a result, it is possible that soluble sugar, starch, and nitrogen in leaves and stems of grafted cuttings synergistically played a positive role in rooting of cut chrysanthemum.
Endogenous phytohormones were thought to be involved in the complex relationship between the rootstock and the scion [26]. The IAA and GA content of grafted cucumber plants were both higher than in the nongrafted ones [27]. In this study, the grafted cuttings had more IAA but less ABA and GA than the nongrafted cuttings (Figure 3), such similar result had been reported in cucumber [28]. Also the IAA/ABA ratio was significantly higher in the grafted cuttings, compared with in that the nongrafted cuttings, while there was no significant difference in the ABA/GA ratio (Figures 3(d) and 3(e)). Yang [11] emphasized that the level of IAA/ABA played an important part in the rooting ability of chrysanthemum, and the cuttings with higher IAA/ABA were easier to rooting. It inferred that higher IAA/ABA ratio in the grafted chrysanthemum contributed to the better rooting ability. Our results showed that the grafting affected the level of various endogenous phytohormones over an extended period in chrysanthemum, and thereby lead to high production of more vigorous cuttings.
In summary, when chrysanthemum scions are grafted onto an A. scoparia rootstock, the resulting plants produce more vigorous cuttings and rooting ability related to an altered physiology. The study has thereby validated the idea of grafting improving the productivity of cutting production in chrysanthemum.