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Rapid phenotyping of HPA‐1a using either diabody‐based hemagglutination or recombinant IgG1‐based assays

BACKGROUND: The HPA‐1 system is carried on the β3 integrin. HPA‐1a (Zwa, PlA1) is immunogenic in an HPA‐1b homozygote (HPA‐1b1b). In pregnancy, 1 of 365 women forms anti‐HPA‐1a, which causes severe thrombocytopenia in 1 in 1100 neonates. Identification of women at risk of forming anti‐HPA‐1a and the screening of donors to obtain HPA‐1a‐negative platelets for therapy need reliable, low‐cost, automated assays.