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Analysis of RNA in Three Different Solvents Fixed Paraffin Embedded Slices

For qualitative and quantitative analysis of RNA in 10% formalin, acetone or Methacarn (methanol chloroform acetic acid) fixed paraffin slices, the kidneys of 6 Wistar rats with 250 g body weight each were used. After fixed by formalin, acetone or Methacarn paraffin embedded and H E stained, the RNA was isolated by different methods such as RNA lysis buffer and TRIZOL reagent. The quality and quantity of RNA was analyzed by RT PCR and quantitative PCR. It was shown that the morphology of tissue was preserved well and 181 bp rat glyceraldehyde 3 phosphate (G3PDH) gene, 565 bp beta actin gene and 100 bp plasminogen activator inhibitor 1(PAI 1) gene could be detected by RT PCR in three different solvent fixed slices with two isolation methods. However, RNA lysis buffer could be considered to be better for isolating more RNA than TRIZOL in three different solvents fixed slices.