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High Force Development and Crossbridge Attachment in Smooth Muscle from Swine Carotid Arteries

In experiments designed to achieve maximal activation, the active force/cell cross- sectional area in tissues prepared from the swine carotid media was 6.7 ± 0.3 (SD) × 105 N/m2. This value exceeds that reported for other vertebrate muscle cells and is striking because of the low smooth muscle myosin content. The hypothesis that high force generation may, in part, reflect an increase in the crossbridge duty cycle, i.e., the fraction of the cycle during which force is generated, was tested by determining the rate of force redevelopment after a step shortening and the ratio of the load-bearing capacity of the contractile system to the developed stress during the course of isometric contractions. Maximal crossbridge cycling rates estimated by the rate of force redevelopment occurred 30 seconds after the onset of a high K+-induced contraction, and decreased thereafter, although the load-bearing capacity or maximum active stress was maintained. These results from isometric experiments support the hypothesis and provide further evidence that attached, noncycling crossbridges contribute to force maintenance in tonically contracting arterial smooth muscle.