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Real‐time fluorescence detection of multiple microscale cell culture analog devices in situ

We investigated multiple microscale cell culture analog (μCCA) assays in situ with a high‐throughput imaging system that provides quantitative, nondestructive, and real‐time data on cell viability. Since samples do not move between measurements, captured images allow accurate time‐course measurements of cell population response and tracking the fate of each cell type on a quantitative basis. The optical system was evaluated by measuring the short‐term response to ethanol exposure and long‐term growth of drug‐resistant tumor cell lines with simultaneous samples.