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The distribution and localization of drug-induced secondary constrictions in human chromosomes.

BUdR, mitomycin C and hydroxylamine hydrochloride induce secondary constrictions in particular human chromosomes. The increased frequency of constriction formation in certain chromosomes is time and drug dependent, and correlates well with the timing of DNA synthesis. The frequency at which some of these constrictions can be induced by specific drugs at specific times increases the usefulness of these landmarks in chromosome identification.