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Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae

Background The production of ethanol and other fuels and chemicals from lignocellulosic materials is dependent of efficient xylose conversion. Xylose fermentation capacity in yeasts is usually linked to xylose reductase (XR) accepting NADH as cofactor. The XR from Scheffersomycesstipitis, which is able to use NADH as cofactor but still prefers NADPH, has been used to generate recombinant xylose-fermenting Saccharomyces cerevisiae. Novel xylose-fermenting yeasts species, as those from the Spathaspora clade, have been described and are potential sources of novel genes to improve xylose fermentation in S. cerevisiae. Results Xylose fermentation by six strains from different Spathaspora species isolated in Brazil, plus the Sp. passalidarum type strain (CBS 10155T), was characterized under two oxygen-limited conditions. The best xylose-fermenting strains belong to the Sp. passalidarum species, and their highest ethanol titers, yields, and productivities were correlated to higher XR activity with NADH than with NADPH. Among the different Spathaspora species, Sp. passalidarum appears to be the sole harboring two XYL1 genes: XYL1.1, similar to the XYL1 found in other Spathaspora and yeast species and XYL1.2, with relatively higher expression level. XYL1.1p and XYL1.2p from Sp. passalidarum were expressed in S. cerevisiae TMB 3044 and XYL1.1p was confirmed to be strictly NADPH-dependent, while XYL1.2p to use both NADPH and NADH, with higher activity with the later. Recombinant S. cerevisiae strains expressing XYL1.1p did not show anaerobic growth in xylose medium. Under anaerobic xylose fermentation, S. cerevisiae TMB 3504, which expresses XYL1.2p from Sp. passalidarum, revealed significant higher ethanol yield and productivity than S. cerevisiae TMB 3422, which harbors XYL1p N272D from Sc. stipitis in the same isogenic background (0.40 vs 0.34 g gCDW−1 and 0.33 vs 0.18 g gCDW−1 h−1, respectively). Conclusion This work explored a new clade of xylose-fermenting yeasts (Spathaspora species) towards the engineering of S. cerevisiae for improved xylose fermentation. The new S. cerevisiae TMB 3504 displays higher XR activity with NADH than with NADPH, with consequent improved ethanol yield and productivity and low xylitol production. This meaningful advance in anaerobic xylose fermentation by recombinant S. cerevisiae (using the XR/XDH pathway) paves the way for the development of novel industrial pentose-fermenting strains. Electronic supplementary material The online version of this article (doi:10.1186/s13068-016-0570-6) contains supplementary material, which is available to authorized users.


Background
The lignocellulosic ethanol technology is reaching the commercial scale [1] and is enabling the industrial production of other bio-based products from lignocellulosic materials. Lignocellulose is the major structural component of plants and consists of cellulose, hemicellulose and lignin [2]. Lignocellulosic substrates are the largest source of fermentable sugars for the production of fuels and chemicals and the economic viability of second generation (2G) or lignocellulosic ethanol technology is dependent on the complete and efficient conversion of the carbohydrates, including those from the cellulosic and hemicellulosic fractions, primarily glucose and xylose [3,4].
One of the key challenges for cost-effective lignocellulosic ethanol is the availability of robust microorganisms able to efficiently ferment all sugars present in lignocellulosic hydrolysates [1]. To address this bottleneck, Saccharomyces cerevisiae, the most commonly microorganism used in industrial alcoholic fermentations, has been engineered towards efficient xylose fermentation capacity [1], since other microorganisms, including native xylosefermenting yeasts, can hardly convert xylose into ethanol at high titers, yields or productivities under industrially relevant ethanol production conditions, such as strict anaerobic environment, high osmotic stress, or high concentration of ethanol and inhibitors present in lignocellulosic hydrolysates [5]. Two metabolic engineering approaches have been followed to confer S. cerevisiae the ability to ferment xylose: the heterologous expression of xylose isomerase (XI) from bacteria or anaerobic fungi, which catalyzes the direct isomerization of d-xylose to d-xylulose [6][7][8]; and the heterologous expression of xylose reductase (XR) and xylitol dehydrogenase (XDH) from native xylose-fermenting yeasts (e.g., Scheffersomyces stipitis), which convert d-xylose into xylitol and xylitol into d-xylulose, respectively [7,9]. The XR from Sc. stipitis uses both NADPH and NADH as cofactor, while XDH is NAD + -dependent enzyme [10]. Under anaerobiosis, engineered S. cerevisiae expressing XR/XDH from Sc. stipitis secrete significant amounts of xylitol due to scarcity of NAD + [11], with a negative impact on the ethanol yield and productivity. To reduce xylitol production, enzyme engineering strategies have been applied to generate XR and/or XDH mutants with increased affinity for NADH and/or NADP + , respectively [12][13][14][15][16]. Among the modification induced or selected, the N272D mutation in XYL1 from Sc. stipitis resulted in an increased preference for NADH in this yeast [14], and a recombinant S. cerevisiae expressing the same gene with N272D was able to grow anaerobically in xylose [15].
Conversely, microbial biodiversity has been a significant source of innovation in biotechnology [17]. Novel (non-conventional) yeasts from various habitats and some of their unique traits are source of biocatalysts for direct application in industrial processes or for metabolic engineering of industrial microorganisms, such as the yeast S. cerevisiae [18,19]. Following extensive investigation on Schefersomyces stipitis and other xylose-fermenting strains, Spathaspora passalidarum was revealed as a novel yeast species capable of efficient xylose fermentation to ethanol [20][21][22]. More recently, five new species from the Spathaspora clade were isolated from Brazilian ecosystems [23,24]. In addition, novel strains of Sp. passalidarum were obtained in Brazil [25]. In this context, the present work aimed to explore the xylose metabolism in these novel Brazilian Spathaspora strains through physiological, biochemical, and molecular characterization and further metabolic engineering of S. cerevisiae to assess the potential on novel traits towards superior xylose fermentation into ethanol under anaerobic conditions.
Spathaspora arborariae achieved ethanol titers of 10.6 and 16.0 g L −1 under moderate and severe oxygen limitation, respectively, but ethanol productivities were much lower than those found with Sp. passalidarum (0.11-0.12 g g −1 CDW h −1 ). Although this yeast generated ethanol as main product, a considerable amount of xylitol was obtained, around 6.4 and 9.0 g L −1 under moderate and severe oxygen-limited conditions, respectively.

Xylose reductase and xylitol dehydrogenase activities in Spathaspora species
The enzyme activities associated with the first steps of xylose metabolism, xylose reductase (XR), and xylitol dehydrogenase (XDH) were determined in Spathaspora species cell extracts after 16 h xylose metabolism under moderate or severe oxygen-limited conditions ( Table 2).

The effect of SpXYL1.1 and SpXYL1.2 expression in Saccharomyces cerevisiae
Within the Spathaspora species studied in this work, Sp. passalidarum is the sole presenting higher XR activities with NADH as cofactor and harboring two XYL1 genes, The strain S. cerevisiae TMB 3422, which carries the mutated Sc. stipitis XR (N272D) [14,15], was used as benchmarking for TMB 3504 ( Fig. 3; Table 4). Maximum specific growth rate (0.11 vs 0.07 h −1 ), specific substrate consumption (0.76 vs 0.58 g g −1 CDW h −1 ) rate, and specific ethanol productivity (0.33 vs 0.18 g g −1 CDW h −1 ) of S. cerevisiae TMB 3504 are significantly higher than those of S. cerevisiae TMB 3422 (Table 4), which in turn reveals a relatively constant ethanol production rate until approximately 100 h, reaching maximal ethanol concentration of approximately 16 g L −1 (Fig. 3a), i.e., an ethanol yield of 0.34 g g −1 . The higher xylose consumption rate, and ethanol yield and productivity revealed by S. cerevisiae TMB 3504 when compared to S. cerevisiae TMB 3422 is linked lower xylitol yield (0.10 vs 0.21 g g −1 ) ( Fig. 3; Table 4).
XR activity was determined in crude cell-free extracts from S. cerevisiae TMB 3504 and TMB 3422 after approximately 48 h of anaerobic batch fermentation (

Discussion
In the present study, a group of Spathaspora species, belonging to a relatively new clade of xylose-fermenting yeasts [20][21][22][23][24][25][26], has been studied in relation to their xylose-fermenting capacity and classified as xylitol or ethanol producers according to the major product of xylose metabolism. This physiological characterization was associated with the biochemical characterization of XR activity: the ethanol producers Sp. passalidarum and Sp. arborariae revealed XR activities with both NADH and NADPH as cofactors; the xylitol producers, Sp. brasiliensis, Sp. roraimanensis, Sp. suhii, and Sp. xylofermentans, showed XR activities strictly NADPHdependent. As Crabtree-negative yeasts in the metabolism of xylose, ethanol production only takes place under oxygen-limited conditions. These conditions impair the efficient regeneration of NAD + required for the xylitol oxidation by XDH, unless XR is able to use NADH as cofactor, thus liberating NAD + for the next reaction catalyzed by XDH. The xylose fermentation capacity of yeasts has been directly correlated with the existence of an XR able to utilize NADH as cofactor [27]. Although, in this work, all the Spathaspora species tested were able to produce more than 2 g L −1 ethanol from xylose (40-50 g L −1 ), except Sp. suhii under severe oxygen-limited conditions, the requirement of an XR able to use NADH as cofactor for efficient xylose fermentation is confirmed. Among the best wild xylose-fermenting yeasts described in the past decades, Pachysolen tannophilus, Scheffersomyces shehatae (including Sc. lignosus and Sc. insectosa) and Scheffersomyces stipitis stood out first, followed more recently by Spathaspora species [20][21][22][23][24][25][26][27][28]. The oxygen-limited conditions applied in this work confirmed Sp. passalidarum as the best xylose-fermenting yeast described so far, with ethanol yields close to the theoretical maximum (0.47-0.48 g g −1 ), a feature only achieved by Sc. stipitis and Sc. lignosus [25,28]. However, Sp. passalidarum showed higher ethanol productivities than these yeasts. Still, as with other natural xylose-fermenting yeasts, the specific aeration requirements and the performance in hemicellulose hydrolysates [26] may difficult the utilization of Sp. passalidarum under industrial setup. The superior xylose fermentation behavior revealed by Sp. passalidarum was suggested to be related to the higher XR activity with NADH than with NADPH [21,22]. This apparent preference for NADH in crude extracts, up to 1.8 of NADH/NADPH ratio [22], is confirmed in this work for both Sp. passalidarum strains tested, a trend that is not followed by the other Spathaspora species. The second major ethanol producer of this study Sp. arborariae revealed higher XR activity with NADPH, as other xylose-fermenting yeasts, such as Sc.
stipitis. Although both Sp. passalidarum strains presented similar NADH/NADPH ratios for XR activity, this activity was 3.5-fold higher in Sp. passalidarum CBS 10155 T .
The alignment of a genome region from the two species classified as ethanol producers, Sp. passalidarum [29] and Sp. arborariae [30], resulted in the identification of two genes coding xylose reductases in Sp. passalidarum, XYL1.1, and XYL1.2, separated by approximately 1 kb, but only one XYL1 in Sp. arborariae. Consensus primers allowed the identification of complete (but apparently single) XYL1 sequences in the remaining Spathaspora species. XYL1.1 and XYL1.2 from both Sp. passalidarum were individually expressed together with Sc. stipitis XYL2 (coding a xylitol dehydrogenase) in S. cerevisiae TMB 3044 [7], a background with overexpressed pathway for xylose utilization and virtual absence of XR activity (Δgre3). The resulting strains, S. cerevisiae TMB 3501 (XYL1.1 from Sp. passalidarum CBS 10155 T ) and S. cerevisiae TMB 3502 (XYL1.1 from Sp. passalidarum UFMG-CM-Y469), were unable to grow on minimal medium containing xylose as sole carbon and energy source under anaerobic conditions and, under aerobiosis, XR activity was, in both cases, strictly NADPH-dependent. On contrary, S. cerevisiae TMB 3503 (XYL1.2 from Sp. passalidarum CBS 10155 T ) and TMB 3504 (XYL1.2 from Sp. passalidarum UFMG-CM-Y469) were able to grow anaerobically in xylose medium, revealing XR activity with dual cofactor utilization with higher activity with NADH (NADH/NADPH ratio 1.2-1.3). Moreover, in Sp. passalidarum, XYL1.2 is 6-16-folds more expressed than XYL1.1 (6-9 for Sp. passalidarum CBS 10155 T and 15-16 for Sp. passalidarum UFMG-CM-Y469) under the (oxygen-limited) conditions tested for transcript analysis. This different expression levels between XYL1.2 and XYL1.1 may explain the overall higher XR activity with NADH found in crude extracts of Sp. passalidarum and the efficiency of this species on xylose fermentation.
The existence of an additional gene in Sp. passalidarum able to provide to this species a specific behavior with respect to D-xylose metabolism may be explained by the gene duplication phenomenon [31][32][33][34], evidenced by the XYL1.1 and XYL1.2 proximity (1 kb distance). Paralogous genes are rarely preserved in the genome and their maintenance is related to the differentiation and acquisition of a functional specialization or neofunctionalization [31,32,34]. The possible duplication of XYL1 in Sp. passalidarum may have enabled an evolutionary adaptation and specialization of XYL1.2 for oxygen-limited xylose utilization, which is much more expressed than XYL1.1 under these conditions. In fact, the expression of duplicated genes tends to evolve asymmetrically, i.e., the expression of one copy evolved rapidly, while the other copy retains most of the expression profile found in the common ancestral gene [35].
The comparison of the XR coding genes as well as the predicted amino acid sequences in Spathaspora species allowed the identification of conserved and divergent regions. Percentages of identities between 89 and 100 % and 93 and 100 % were found by comparing, respectively, the sequences of XYL1 and XYL1.1, and XYL1p and XYL1.1p among the Spathaspora species studied with the type strain of Sp. passalidarum. These results demonstrate a high degree of sequence conservation among the genes and proteins from the species analyzed. Interestingly, XYL1 from Sp. xylofermentans shows 100 % identity with XYL1.1 of Sp. passalidarum (type strain). Interestingly, XYL1p (XRs) can be grouped according to their cofactor utilization profile: (1)  From the analysis of amino acid residues, 115 residues differ among groups, 44 are exclusively found in NADH-preferring XR sequences (XYL1.2p from Sp. passalidarum), and 1 in those able to use NADH (groups 2 and 3), which corresponds to a lysine (K) residue instead of an arginine (R) in position 59/60. Moreover, adjacent to the cofactor binding site of the enzyme, the highly conserved tetrad Ile-Pro-Lys-Ser (IPKS) [11,[33][34][35], in position 271/272, XYL1.2p from Sp. passalidarum display an aspartate (D) residue instead of asparagine (N) found in the remaining XYL1p [except that from Sp. brasiliensis, which displays a serine (S)] (Additional file 2). Protein engineering has been applied to modify cofactors preference of XRs [11][12][13][14][15]. Among the modification induced or selected, the N272D mutation in XYL1 from Sc. stipitis resulted in an increased affinity for NADH in this yeast [13], and a recombinant S. cerevisiae expressing the same gene with N272D was able to grow anaerobically in xylose [14,15].
This strain, S. cerevisiae TMB 3422 [14], was used as benchmarking for the characterization of the newly generated S. cerevisiae TMB 3504 (expressing XYL1.2p from Sp. passalidarum UFMG-CM-Y469) in anaerobic xylose fermentation, as these strains have an isogenic background. The S. cerevisiae TMB 3504 strain was able to efficiently convert d-xylose into ethanol with high yield and productivity and low xylitol accumulation. Compared to S. cerevisiae TMB 3422, the recombinant xylose-fermenting S. cerevisiae considered by Runquist et al. [15] as responsible for one of the highest ethanol yields already reported for engineering S. cerevisiae harboring the XR/XDH pathway, S. cerevisiae TMB 3504 displays approximately more 20 % ethanol yield (0.40 vs. 0.34 g g −1 ), less than 50 % xylitol yield (0.10 vs. 0.21 g g −1 ), and 80 % higher specific ethanol productivity, corresponding to a maximum ethanol titer of approximately 18 g L −1 after 72 h (from approx. 50 g L −1 xylose), almost half of the fermentation time observed for S. cerevisiae TMB 3422. This significantly higher volumetric productivity found for S. cerevisiae TMB 3504 is, in part, explained by the higher biomass yield (0.06 vs. 0.04 g g −1 in S. cerevisiae TMB 3422).
The major bottlenecks in recombinant xylose-fermenting S. cerevisiae are low ethanol productivity from xylose [15], poor xylose fermentation in the presence of inhibitors [36], poor xylose co-consumption with glucose [37], and, in those expressing the XR/XDH pathway, the considerable xylitol accumulation and consequent limited ethanol yield [7,38]. To avoid xylitol accumulation, the majority of industrial xylose-fermenting strains have been constructed using the XI pathway [36], but the novel metabolic engineered laboratory S. cerevisiae strain (TMB 3504), harboring an XR with apparent preference for NADH, showed much lower xylitol accumulation together with a higher ethanol yield and productivity when compared to other recombinant XR/XDH xylosefermenting S. cerevisiae strains [15].

Conclusions
This work identifies crucial traits for xylose fermentation in Spathaspora genus, through physiological, biochemical, and molecular characterization and further functional expression of XRs in S. cerevisiae. The characterization of xylose fermentation, XR/XDH activities, and the identification and characterization of the XR coding genes (XYL1) allowed the selection of two genes, one from each Sp. passalidarum studied, coding for XR activity with apparent preference for NADH. Saccharomyces cerevisiae TMB3504 is a new recombinant xylose-fermenting strain harboring XYL1.2 from Sp. passalidarum UFMG-CM-Y469. This strain revealed an ethanol yield of 0.40 g g −1 with specific productivity of 0.33 g g −1 CDW h −1 against 0.34 g g −1 and 0.18 g L −1 h −1 found with the reference strain used (S. cerevisiae TMB3422), previously reported as one of the best recombinant xylose-fermenting lab strain harboring an heterologous XR/XDH pathway (protein engineered XR from Sc. stipitis).
The rational bioprospective approach undertaken revealed XYL1.2 from Sp. passalidarum as a key for efficient xylose anaerobic fermentation by S. cerevisiae when expressing the XR/XDH pathway. The results obtained with the novel laboratory xylose-fermenting S. cerevisiae strain bring new insights to the XR/XDH pathway as an alternative for industrial strain development towards the effective deployment of lignocellulosic ethanol.  [23]. The other Brazilian Spathaspora strains were isolated from the same substrate in the Amazonian Forest, as described by Cadete et al. [24]. Spathaspora passalidarum CBS 10155 T was bought from the Centraalbureau voor Schimmelcultures (CBS) culture collection. Saccharomyces cerevisiae strains TMB 3044 and TMB 3422 were provided by the Teknisk Mikrobiologi (TMB) culture collection (Lund, Sweden). All strains were stored in 15 % glycerol at −80 °C.

Xylose fermentations under oxygen-limited conditions with Spathaspora species
Fermentation experiments were carried out on YPX medium (yeast extract, 10 g L −1 ; peptone, 20 g L −1 ; d-xylose, 40-50 g L −1 ) in shake flasks under the preestablished oxygen-limited conditions, being (1) moderate: 100 mL working volume in 250 mL cotton plugged flask and (2) severe: 100 mL working volume in 100 mL rubber plugged flask with needle to allow the release of CO 2 . Oxygen transfer rate (OTR) was determined by direct measurements in the culture media (with or without cells), at several time intervals, with an oxygen electrode after sparging with nitrogen. Yeasts were pregrown on YMA medium (glucose, 10 g L −1 ; peptone, 5 g L −1 ; yeast extract, 3 g L −1 ; malt extract, 3 g L −1 ; agar, 20 g L −1 ) for 24-48 h, and single colonies were transferred to 50 mL YPX in 250 mL shake flasks at 30 °C and 200 rpm. Cells were recovered by centrifugation at 2600g for 20 min, washed twice with sterile water, and suspended in the fermentation media as inoculum with a final concentration of 0.5 g CDW L −1 [25]. The flasks were incubated at 30 °C and 200 rpm, and the fermentation was monitored by taking samples between 0 and 144 h, according to the conditions of oxygen limitation employed. Samples were stored at −20 °C until analysis. All experiments were performed in duplicate. Cell biomass was determined by correlating optical density (OD) at 600 nm (OD 600 ) in a Thermo Spectronic Genesys 20 Model 4001/4 spectrophotometer (Thermo Scientific, Waltham, USA) with the cell dry weight (CDW), by means of a calibration curve previously constructed for each yeast strain. Xylose, xylitol, glycerol, acetate, and ethanol were analyzed by a high-performance liquid chromatography system (Merck Hitachi, Darmstadt, Germany), equipped with an Aminex HPX-87H column (Bio-Rad Hercules, USA) and a refractive index detector (L-7490, Merck Hitachi, Darmstadt, Germany). The column was eluted with 5 mM H 2 SO 4 as mobile phase at a flow rate of 0.4 mL min −1 , at 50 °C. Fermentation parameters [Y et p/s (g g −1 ), ethanol yield; Y xy p/s (g g −1 ), xylitol yield; Y x/s (g g −1 ), biomass yield; r s (g g −1 CDW h −1 ), specific d-xylose consumption rate, d-xylose consumption (%), r et (g g −1 CDW h −1 ), specific ethanol productivity] were experimentally determined. Ethanol (Y et p/s , g g −1 ), xylitol (Y xy p/s g g −1 ) and biomass (Y x/s , g g −1 ) yields were calculated by correlating ΔP produced (ethanol or xylitol) or ΔX produced (CDW) with ΔS consumed (d-xylose) at time of maximum ethanol production, respectively; specific ethanol productivity (r et , g g −1 CDW h −1 ) was determined during maximum volumetric ethanol production rate divided by the average of the biomass (CDW) in the same time interval; similarly, specific d-xylose consumption rate (r s , g g −1 CDW h −1 ) was calculated during maximum volumetric d-xylose consumption rate divided by the average of the biomass (CDW) in the same time interval, and d-xylose consumption (%) was determined as a percentage of initial d-xylose consumed at time of maximum ethanol production.

Enzyme activities
For the enzymatic activity assay of XR and XDH in Spathaspora species, yeasts were grown in YPX medium as described above under both oxygen-limited conditions tested. After 16 h, cells were recovered, washed with sterile deionized water, and used to obtain crude cell-free extracts using Y-PER ® Yeast Protein Extraction Reagent (Pierce, Rockford, USA). Protein concentrations in the cell-free extract were determined by BCA Protein Assay Kit (Pierce, Rockford, USA). Enzymatic activities were determined by following the oxidation or reduction of the coenzymes at 340 nm using UV-2401 PC UV-PIS recording spectrophotometer (Shimadzu, Kyoto, Japan) at 25 °C, with an interval time of 1 s for recording and a total measuring time of 90 s for each reaction. Kinetic parameters of XR for xylose reduction were obtained in a reaction mixture containing 200 mM triethanolamine buffer pH 7.0, 10 mM NAD(P)H, 2 M d-xylose, cell-free extract and deionized water, while kinetic parameters of XDH for xylitol oxidation were obtained in a reaction mixture containing 200 mM glycine buffer pH 9.0, 500 mM MgCl 2 , 60 mM NAD(P) + , 2 M xylitol, cell-free extract, and deionized water. A value of 5.33 mM −1 cm −1 was used for the absorption coefficient of NAD(P)H. One unit was defined as the generation of 1 μmol NAD(P) H per min. The specific enzyme activities were given in units (U) per mg protein [39]. This experiment was performed in biological duplicate.
Enzymatic activities of XR in TMB strains (3501, 3502, 3503, and 3504) grown in aerobic conditions in shake flasks containing 2× YNB medium with 50 g L −1 xylose and 50 mM potassium hydrogen phthalate pH 5.5, at 30 °C and 200 rpm for 48 h, were also performed. Reaction mixtures and the calculations of specific enzyme activities were conducted as described above for determination of Spathaspora species XR activities.

Genomic analysis of XYL1 gene(s) in Spathaspora species
The genome regions of 15 Mbp containing XYL1 gene(s) from Spathaspora passalidarum NRRL Y-27907 T (CBS 10155 T ) [29] and Sp. arborariae UFMG-CM-Y352 T (UFMG-HM-19.1A T , CBS 11463 T ) [30] were aligned and two sets of primers were designed (Additional file 3): one based on a consensus sequence covering XYL1.1 of Sp. passalidarum and XYL1 of Sp. arborariae, (SpspXYL1.1_F and SpspXYL1.1_R), previously assigned [29,30]; and one covering XYL1.2 of Sp. passalidarum (SppaXYL1.2_F and SppaXYL1.2_R), previously assigned in Sp. passalidarum [29]. The amplified fragments were submitted to DNA sequencing (STAB Vida, Portugal) to confirm XYL1.1 gene sequence in Sp. passalidarum CBS 10155 T , XYL1 gene sequence in Sp. arborariae UFMG-CM-Y352 T , and to identify XYL1 genes sequences in the remaining strains. The sequences obtained were used to predict the amino acid residues forming the XR proteins and nucleotide and amino acid sequences were aligned by ClustalW multiple alignment using the free software BioEdit Sequence Alignment Editor (Ibis Biosciences). A phylogram of XYL1p was constructed with the software iTOL (EMBL, Germany).

Transcript analysis of XYL1.1 and XYL1.2 expression in Spathaspora passalidarum
Samples from Sp. passalidarum cultures (CBS 10155 T and UFMG-CM-Y469) were taken for the transcript analysis of XYL1.1 and XYL1.2 along with samples for enzyme activities experiments (i.e., YPX medium, under moderate and severe oxygen-limited conditions, after 16 h). Samples were immediately frozen in liquid nitrogen, and stored at −80 °C prior to extraction. RNA extraction was performed with Direct-zol ™ RNA MiniPrep w/TRI-Reagent ® . First-Strand cDNA Synthesis was obtained with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with the following cycle: 25 °C 10 min, 37 °C 120 min, and 85 °C 5 min, in C1000 Touch ™ Thermal Cycler (BIORAD). This step was performed in duplicate with real-time Power SYBR ® Green PCR (Applied Biosystems) with the following cycle: 94 °C 3 min, 40 cycles of 94, 55, and 72 °C, 0.5 min each temperature, and 72 °C 4 min. The mRNA expression levels of SppaXYL1.1 and SppaXYL1.2 were evaluated using actin (SppaACT1) and 18S ribosomal RNAs (SppaRDN18) as internal controls (primers list in Additional file 3), and analyzed by the Pfaffl method [40].

Xylose fermentations under anaerobic conditions with recombinant S. cerevisiae
Anaerobic batch fermentation was carried out in a flat-bottomed 1.4-l Multifors bioreactor vessel (Infors AG, Bottmingen, Switzerland) with a working volume of 800 mL. Cells were pre-cultivated at 30 °C and 180 rpm in 250 mL shake flasks containing 50 mL of 2× YNB medium (Yeast Nitrogen Base w/o amino acids, 13.4 g L −1 ) with 50 g L −1 d-xylose and 50 mM potassium hydrogen phthalate pH 5.5, recovered by centrifugation at 2600×g for 20 min, washed twice with sterile water, and inoculated into the bioreactor to a final concentration of 0.07 g CDW L −1 . Fermentation was conducted on 2 × YNB medium with 50 g L −1 d-xylose, 0.01 g L −1 ergosterol and 0.5 mL L −1 antifoam RD emulsion (Dow Corning ® , Midland, USA). Temperature was maintained at 30 °C, pH was controlled at 5.5 through addition of 3 M KOH, and stirring was set to 200 rpm. Anaerobic conditions were attained by sparging with nitrogen gas containing less than 5 ppm O 2 (AGA GAS AB, Sundbyberg, Sweden) at a flow rate of 200 mL min −1 before inoculation. During fermentation, anaerobic conditions were maintained by the produced CO 2 that diffused through a water lock. Cultures were sampled aseptically between 0 and 142 h and stored at −20 °C until analysis. Strain S. cerevisiae TMB 3422 [14], carrying Sc. stipitis XYL1 (N272D), was used as reference for S. cerevisiae TMB 3504 carrying native Sp. passalidarum UFMG-CM-Y469 XYL1.2. The experiments were performed in biological duplicates. Growth was determined by measuring OD 620 with a Ultrospec 2100 pro spectrophotometer (Amersham Biosciences, Uppsala, Sweden). Xylose, ethanol, xylitol, glycerol, and acetate were analyzed by highperformance liquid chromatography system (Waters, Milford, USA) with an Aminex HPX-87H ion exchange column (Bio-Rad Hercules, USA) and a refractive index detector (RID-6A, Shimadzu, Kyoto, Japan). The mobile phase was 5 mM H 2 SO 4 , at a flow rate of 0.6 ml, 45 °C. Cell dry weight was determined in triplicate by filtering a known volume of culture broth through a pre-weighed 0.45 μm Supor ® 450 Membrane filters (Pall Corporation, Port Washington, USA), washing with distilled water, drying in a microwave oven, and weighting. Fermentation parameters were calculated as described above.

Authors' contributions
RMC participated in the experimental design, performed yeast physiological and biochemical characterization (fermentation and enzymatic assays),

Additional files
Additional file 1. Time course of substrate consumption (xylose at an initial concentration of 40-50 g L −1 ) and product formation by Spathaspora species under moderate and severe oxygen-limited batch fermentations.
Additional file 3. List and sequence of primers used in this study. This work molecular identification of genes, sequence analysis, molecular biology and strain engineering, data analysis, and drafted the manuscript. AMH and AGS contributed equally, participating on strain engineering and its characterization and commented the manuscript. CF participated in real-time RT-PCR assays. FG participated in the initial planning and commented the manuscript. MFGG participated in the experimental design and analysis of strain engineering and its characterization and edited the manuscript. CR participated in initial planning, participated in the experimental design of the yeast physiological and biochemical characterization and commented the manuscript. CF planned the study, participated in the experimental design and edited the manuscript. All authors read and approved the final manuscript.