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Immunohistochemical study of substance P in the throat and lungs in cases died from anaphylactic shock

Objective To explore the morphological evidence in deaths from anaphylactic shock for medico-legal investigation. Methods 15 cases died from anaphylactic shock were selected as experiment group,and 20 cases died from traffic accident as control group.HE stain for histopathology and immunohistochemical stain for substance P were employed. The image analysis for SP was conducted by BI-2000 image analyzer,and the positive indexes(PI) were calculated.The data were statistically analyzed by Student's t test using SPSS 10.0 for Windows.Results Substance P was detected in throat squamous epithelium and tunica mucosa in bronchial epithelium in the experiment group,which was scarcely seen in the control group.Conclusion Immunohistochemical stain for SP may provide a morphological evidence for medico-legal investigation in cases with suspected anaphylactic shock.