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Conformation of Proflavine-Bound DNA Molecules

Abstract Proflavine, DNA, Electron Microscopic Study The conformational changes suffered by full-length double-stranded DNA molecules from bac teriophage T7, due to their binding with the mutagenic dye proflavine (PF), has been studied by electron microscopy. The dye-bound DNA showed a smoother contour and a more compact con figuration in comparison with the dye-free native DNA. These changes resulted from the stretching, extension and charge-neutralization accompanying the incorporation of PF.