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Structural organization of the tissue-specific middle repetitive sequence of the mouse genome.

The structural genes closely linked to the particular middle repetitive sequence (MRS) expressed in liver nuclei were cloned from the mouse genomic library. From one-fourth of 3,200 MRS-containing clones, 21 clones were obtained as mRNA coding sequence-linked MRS clones. From examination of the structural organization and specificity of expression of the MRS and the mRNA coding sequence, it was concluded that expressions of the MRS and the structural genes closely linked to the MRS are independently regulated.