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The transcriptome of the moss Physcomitrella patens : comparative analysis reveals a rich source of new genes

Three cDNA collections covering all important steps of the life cycle of the moss Physcomitrella patens have been used to prepare three normalized and subtracted cDNA libraries for the purpose of mass sequencing. After production of around 110,000 expressed sequence tags a clustered database was built and analysed. The Physcomitrella transcriptome is estimated to contain around 25,000 genes. Whereas those cover around 50% of the Arabidopsis gene content (around 26,000 genes), Physcomitrella possesses more than 11,000 expressed protein coding genes that cannot be identified by terms of sequence homology to date.