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Detection and Distribution of Intestinal Calcium-Binding Protein in the Young Adult Male Guinea Pig 1

Summary A calcium-binding factor was detected in guinea pig intestinal mucosa. It was negatively charged and moved in an electrical field. Pronase quickly destroyed almost completely the calcium-binding activity although trypsin had practically no effect. The major calcium-binding peak was well defined. It was concluded that the factor is probably a protein. Calcium-binding protein was found throughout the gastrointestinal tract, with the calcium-binding activity decreasing with increasing distance from the stomach. The authors thank Dr. Walter L. Nelson for supplying the guinea pigs and Mr. Francis Davis and Mrs. Marie Brindak for their excellent technical assistance.