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[Characteristics of the immobilization and transformation of sterols by Mycobacterium phlei 1026 cells incorporated into PAAG].

Experiments were carried out to study the effect of polymerization conditions and polyacrylamide gel structure on the enzyme activity of Mycobacterium phlei immobilized cells degrading sterols to C19-steroids. The modified sterol 4-chlostene-3(O-carboxymethyl)oxime was used as substrate. The polymerization conditions (the number of the cells incorporated and the reaction temperature) significantly affected the ability of the immobilized cells to degrade the sterol side chain. The monomer contact produced no negative effect on the cellular enzymic activity. The following conditions of immobilization and transformation appear to be optimal: cell incorporation into 10% gel, monomer (AA:MBA) ratio 95:5, polymerization temperature 2-8 degrees C, polymerization mixture biomass 40 mg/ml, granule size about 1 mm. Enzymic activity can be substantially increased via cell incubation in the nutrient medium.