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ACTION OF SENZOYETHIAMINE MONOPHOSPHATE ON YEAST 1

Recently new derivatives of thiamine and other vitamins have been synthesized and the experiments using microorganisms as the tests for their biological effects have been carried out. For investigating the biological activities of vitamin derivatives partially different in structure from the vitamin, it must be taken into consideration whether their effects on microorganisms reflect the activities of animals, especially on human beings or not. Further, if the effects were found different from the vitamin, it should also be considered whether the cause is due to the difference in permeability or affinity to microorganism cells. S-Benzoylthiamine monophosphate (BTMP) is a thiol form of thiamine mono phosphate in which a benzoyl group is attached to the sulfur atom forming a thioester (Fig, 1), It is of interest that BTMP has both lipophilic and hydrophilic groups in the same molecule. The authors intended to clarify the following problems: (a) whether BTMP shows an vitamin action on yeast; (b) if so, whether it is effective as itself; (c) whether the rate of appearance of the effect due to permeability and affinity to yeast cells is different from that of thiamine,