{'fraction_non_alphanumeric': 0.037148508040244214, 'fraction_numerical': 0.031473041534095796, 'mean_word_length': 4.754576942107867, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 3, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The high potential of Pelargonium roseum plant for phytoremediation of heavy metals

The major objective of this investigation was to evaluate the potential of scented geraniums, Pelargonium roseum, to uptake and accumulate heavy metals nickel (Ni), cadmium (Cd), or lead (Pb). For this, plants were grown in an artificial soil system and exposed to a range of metal concentrations over a 14-day treatment period. Then, metals from the entire biomass were extracted. The results showed that scented geranium plants accumulated in excess of 20,055 mg of Ni kg−1 dry weight (DW) of root and 10,889 mg of Ni kg−1 DW of shoot, and in excess of 86,566 mg of Pb kg−1 DW for roots and 4,416 mg of Pb kg−1 DW for shoots within 14 days. Also, the uptake and accumulation of cadmium in roots of scented geranium plants increased with the exposure at low (250, 500 mg L−1) and medium level (750 mg L−1) followed by a decline at the highest level (1,000 mg L−1). The highest accumulation in roots (31,267 mg kg−1 DW) was observed in 750 mg L−1 cadmium treatment. In the shoots of scented geraniums, the highest amount of metal accumulation (1,957 mg kg−1 DW) was detected at 750 and 1,000 mg L−1 of cadmium in the culture solution. Finally, since the high concentrations of Ni or Pb accumulated in shoots of scented geranium has far exceeded 0.1 % DW and for Cd has far exceeded 0.01 % DW, P. roseum is a new hyperaccumulator species for these metals and can be used in phytoremediation industry.


Introduction
Sites where soils are contaminated with heavy metals are commonly found as a result of activities such as metal mining and refining, specific industrial processes or the long-term disposal of wastes such as sewage sludge. Elevated concentrations of heavy metals in soils may cause phytotoxicity, direct hazard to human health, indirect effects due to transmission through the food chain or contamination of ground or surface waters.
Clean-up technologies have been developed for the removal of heavy metals (Luo 2009), but often these are expensive or have some environmentally deleterious consequences. Soil washing techniques, both in situ and ex situ, aim to remove metals from soil but have detrimental effects on their physical and biological properties and result in a waste product with a high metal content, which itself requires disposal.
Phytoremediation, the use of plants to reduce the risk associated with heavy metal contaminated soils, is now being actively investigated as a low-cost option that causes no deterioration in soil quality. The idea originated from the identification of hyperaccumulator plants, which can take up large amounts of heavy metals and accumulate high concentrations in their above ground tissues with no adverse effects on growth (Baker et al. 1994;Chaney et al. 1997;Reeves and Baker 2000).
Currently, the defining plant tissue concentrations for hyperaccumulation are Cd >100 mgkg −1 ; Co, Cu, Ni, Pb >1,000 mgkg −1 ; Mn, Zn >10,000 mg kg −1 . However, plants with such a large accumulation of heavy metals typically produce relatively small amounts of biomass and have no economic value (Gleba et al. 1999). Thus, identification of new plant species with a high biomass and a robust growth habit, coupled with ability to tolerate and accumulate multiple metals, has become an important aspect of phytoremediation.
The overall objective of the current investigation was to evaluate the potential of the scented geranium plants, Pelargonium roseum, to uptake and accumulate major metal pollutants, nickel, cadmium, and lead from growth substrate.

Preparation of test material
Scented geranium rooted cuttings (Pelargonium roseum) were used for all experiments in this study. Cuttings of scented geraniums made from greenhouse-grown plants were rooted in plastic pots containing approximately 100 g of wellwashed coarse vermiculite.
Plants (one plant in each pot) were placed in plastic saucers (three pots in each saucer, two saucers per treatment, and six plants per treatment). The plants were grown in a greenhouse equipped with supplementary lighting (16/8 h photoperiod; 15 Klux) and controlled temperature (18-22°C) for 45 days with irrigation and fertilization.

Metal exposure
During the treatment period (14 days), the rooted cuttings were treated with 50 mL of half-strength Hoagland ' s solution (Hoagland and Arnon 1950) devoid of phosphates and sulphates but supplemented with the metal salt. The metal treatments were applied individually and provided as nitrate: 0, 250, 500, 750, 1,000 mgL −1 Ni(NO 3 ) 2 .4H 2 O or Cd(NO 3 ) 2 .4H 2 O and 0, 500, 1,000, 1,500, 2,000, 2,500 mgL −1 Pb(NO 3 ) 2 , and the pH of the metal solutions was adjusted to 6.0-6.5. The solutions were applied in the saucers and repeated at 24-h intervals, over the 14-day treatment period. The experiments were repeated at least twice.

Metal analysis
Plants from all the treatments were harvested at the same time, washed thoroughly with distilled deionized water, and divided into root and shoot biomass. Metal from the entire biomass was extracted using the closed Teflon vessel method as described by Topper and Kotuby-Amacher (1990). Briefly, the tissue was cut into small pieces and oven dried at 80°C for 2 days. Oven-dried materials were weighted, and a sample of 1 g each was placed in Teflon vessels. The plant material was digested by adding 30 mL of trace metal grade nitric acid (70 %, Merck), and the Teflon vessels were placed in an oven overnight (110°C). The metal content in the extract was estimated using a flame atomic absorption spectrophotometer, Model Perkins-Elmer. Standard solutions were used to assess the concentrations of samples.

Statistical analysis
Analysis of variance (one-way ANOVA) was carried out using the Statistical software, SPSS, 11 to determinate if there were significant differences in metal accumulation as a result of metal treatments. Significant differences between the means assessed by Duncan test at P<0.05.

Results
Exposure of scented geraniums to various concentrations of Ni, Cd, or Pb induced morphotoxicity symptoms on the plants exposed to the higher levels (Fig. 1).
The symptoms were mostly observed on the mature leaves (at 12 days), which indicated signs of chlorosis and early senescence. In addition, these plants were shorter than control plants (Fig. 1).
The uptake and accumulation of nickel in roots of scented geranium plants increased with the exposure at low (250, 500 mgL −1 ) and medium level (750 mgL −1 ) followed by a decline at the highest level (1,000 mgL −1 ). The highest accumulation in roots (20,055 mgkg −1 DW) was observed in the medium (750 mgL −1 ) nickel treatment (Fig. 2a).
In the shoots of scented geraniums at the low levels of nickel exposure, the amount of metal accumulated reached 882 and 5,649 mg kg −1 DW. The highest amount of metal accumulation (10,889 mgkg −1 DW) was detected at 750 and followed by a decline at the highest level (1,000 mgL −1 ) of nickel in the culture solution (Fig. 2b).
The results of this research showed that the uptake and accumulation of lead (Pb) in shoot and root followed a similar trend (Fig. 3). The highest amount of Pb accumulation was detected at 2,500 mgL −1 lead nitrate in culture solution that was 86,566 mgkg −1 DW for roots (Fig. 3a) and 4,416 mgkg −1 DW for shoots (Fig. 3b).
In the case of cadmium, results showed that the uptake and accumulation of this heavy metal in roots of scented geranium plants increased with the exposure at low (250, 500 mgL −1 ) and medium level (750 mgL −1 ) followed by a decline at the highest level (1,000 mgL −1 ) (Fig. 4a). The highest accumulation in roots (31,267 mgkg −1 DW) was observed in 750 mgL −1 cadmium treatment (Fig. 4a).
In the shoots of scented geraniums, the highest amount of metal accumulation (1,957 mgkg −1 DW) was detected at 750 and 1,000 mgL −1 of cadmium in the culture solution (Fig. 4b).

Discussion
Phytoremediation of heavy metals has gained a lot of attention in last decades (Reeves and Baker 2000). Plants vary in their ability to remediate and uptake heavy metals. For efficient phytoremediation, screening and selection of hyperaccumulator plants especially for important heavy metals like Pb, Cd, and Ni are  necessary. Thus, identification of new plant species with a high biomass and a robust growth habit, coupled with ability to tolerate and accumulate multiple metals, has become an important aspect of phytoremediation.
Pelargonium roseum is a fragrance and ornamental plant and grows on wide geographical locations in world. In this study, we showed its high efficiency for remediation of Pb, Ni, and Cd heavy metals. The high concentration of Ni and Pb found in the shoots of scented geranium plants has far exceeded 0.1 % DW and 0.01 % for Cd which are considered as a standard for defining Ni, Pb, or Cd hyperaccumulator plants in a natural environment (Chaney et al. 1997), and it is likely be a new hyperaccumulator plant.
The linear pattern of uptake in this plant suggests the involvement of both active and passive transport mechanisms for lead uptake.
The higher metals content found in the roots of metal accumulators is highly dependent on at least two components, namely: sequestration and/or translocation.
Nickel and cadmium sequestration in roots has been associated with the formation of cell wall ingrowths , precipitation of metal oxalates in root intercellular spaces (Van Balen et al. 1980), and apoplastic storage . Alternatively, nickel and cadmium binding to low molecular weight peptides and proteins (Weigel and Jager 1980) has also been suggested to be involved in metal sequestration in roots. Metal transport to the shoot primarily takes place through the xylem. The metal loading into the xylem sap of Brassica juncea displays biphasic saturation kinetics (Salt et al. 1995), suggesting that xylem loading of metal ions is facilitated by specialized membrane transport processes. Movement of metal ions, particularly cadmium, in xylem vessels appears to be mainly dependent on transpiration-driven mass flow (Salt et al. 1995).
Because xylem cell walls have a high cation exchange capacity, they are expected to retard severely the upward movement of metal cations. Therefore, non cationic metal-chelate complexes, such as nickel-citrate, should be transported more efficiently in transpiration stream (Senden et al. 1990). Theoretical studies have predicted that the majority of the Fe (II) and Zn (II) in xylem sap should be chelated by citrate, whereas Ni (II) should be chelated by various amino acids including histidine and asparagines (Senden et al. 1990). Isolation of a citrate nickelate (II) complex from the latex of the Ni hyperaccumulator Serbertia acuminata supports the role of organic acids in metal transport (Lee et al. 1977).
X-ray absorbance fine structure (EXAFS) analysis showed that Cd in xylem sap of B. juncea was chelated by oxygen or nitrogen atoms, suggesting the involvement of organic acids in nickel translocation (Salt et al. 1995). EXAFS analysis produced no evidence for sulfur coordination of nickel, confirming that phytochelatins and other thiol-containing ligands play no direct role in nickel transport in the xylem.

Conclusion
In the present investigation, we have demonstrated the high potential of scented geranium for uptake and accumulation of heavy metals. This along with earlier reports on lead accumulation in scented geranium plants (KrishnaRaj et al. 2000) indicates the efficacy of different species of this genus for decontamination of multiple metal-polluted sites in phytoremediation industry.