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Aspects of hepatic calcium metabolism.

Pool size analyses have cast some light on the distribution of calcium in different compartments of hepatocytes. The largest amount of cellular calcium, about 800 nmol/gm of liver, was localized to the plasma membrane, where it is bound mainly to glycoconjugates, acidic phospholipids, and neuraminic acid residues. Two classes of calcium-binding sites have been found: higher-affinity sites (K d =4.0×10 3 M −1 ) with 22 nmol of calcium/mg of membrane protein, and lower-affinity sites (K d =3.2×10 2 M −1 ) with 120 nmol of calcium/mg of membrane protein