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International Journal of HIV/AIDS and Research (IJHR) ISSN 2379-1586 The Relationships between Cholesterol, Tobacco and Alcohol Use : A New Opportunity for Intervention among People Living with HIV

Objective: Given increased evidence of cholesterol’s role in the structure and functioning of the central nervous system, this study examines how lipid disturbances might deregulate drinking behaviors. Methods: Using a clinical cohort, the relationship between hypocholesterolemia and alcohol use was prospectively assessed over a period of 6 months while controlling for biological, familial, and intrapersonal factors. People Living with HIV (PLWH) were stratified according to baseline cholesterol levels: hypocholesterolemic (HypoCHL<150mg/dl) or non-HypoCHL (>150mg/dl). We used a Bayesian network model to analyze the probability that HypoCHL predicts alcohol trajectories. Results: At baseline, those participants with HypoCHL consumed more alcohol (29.9 ± 4 vs. 15.1 ± 2 drinks p =0.001) and on more days/week (4 ± 2.7 vs. 2.8 ± 2.5, p =0.002) than normal CHL participants. At the follow-up evaluation, alcohol consumption increased or remained constant in 60% of the sample and was associated with four risk factors: HypoCHL (OR = 1.5, p=0.04), cigarette smoking (OR = 2.9, p = 0.008), drinking liquor (OR = 1.9, p = 0.05), and having a close relative with hazardous alcohol use (OR =3, p=0.0001). The Bayesian model further predicted that if a subject has a baseline cholesterol level below 150 mg, that there would be an 87% probability of maintaining or increasing alcohol intake, and only a 14% chance of reducing intake during the study. Noteworthy, if cholesterol was modeled to be normal, the probability of reducing alcohol intake increased to 41%. Conclusion: This study provides evidence that HypoCHL is a significant predictor of increasing or continuing hazardous consumption of alcohol in PLWH.


Introduction
Hazardous Alcohol Use (HAU) is recognized as a worldwide epidemic. In 2011, the World Health Organization (WHO) reported that the harmful use of alcohol resulted in 2.5 million deaths annually, and ranked as the third leading risk factor contributing to the global burden of disease, followed by unsafe sexual behaviors and being underweight in childhood [1]. Approximately 17.6 million (8.5%) adults meet the diagnostic criteria for alcohol use disorders in the United States. Although alcohol use is common throughout the U.S. population, data has shown that the rates are even higher among People Living with HIV (PLWH), significantly complicating prognosis and diminishing an individual's quality of life [1,2]. Additional evidence shows that HAU interferes with adherence and antiretroviral drug response, accelerating disease progression and promoting liver failure [2][3][4][5][6][7][8][9]. Due to the fact that HAU is a major avoidable risk factor in global disease and disability, particularly among PLWH, this topic poses high relevance to future HIV care and treatment. Clarification of this issue is also necessary to achieve maximum benefits for the success and sustainability of our efforts to control transmission and community viral load [10].
Given CHL's effects on neuropsychological well-being and the neuromodulators described above, the pressing question is whether a relationship between cholesterol level and alcohol consumption exists, and if so, if that can be used to develop more effective evidence-based initiatives.
In seeking to answer these paradigms and to test our proposed model (depicted in Figure 1), a longitudinal study with PLWH was performed. Non-ambulatory patients were excluded, as well as those with major comorbidities, including but not limited to, CNS opportunistic infections, head injuries with or without loss of consciousness, tumors, major psychiatric diseases, developmental disorders, severe malnutrition or confirmed cardiovascular or immune based disease (i.e., malignancies, autoimmune diseases, arthritis). Given that our main goal was to establish the health effects of alcohol, dependent drug users and subjects reporting injection drug use were excluded to reduce their confounding effects. We also excluded any participants who had cirrhosis, active viral hepatitis, or liver enzymes two standard deviations above the normal values.

Subjects, Setting and Procedures
Those participants who expressed a willingness to participate and to provide written informed consent, as well as a medical release, were consecutively enrolled and followed over a period of 6 months. The Institutional Review Board at the University of Miami approved the study. Upon enrollment, participants completed standardized research questionnaires, a brief physical exam, and laboratory tests. Of the total 150, 130 participants completed the two required visits and fulfilled the data portion of the study. Five other subjects were excluded from the analyses because they were taking lipid lowering medications or were on a diet regimen.

Outcome Variable: Alcohol Use
At each visit, participants reported alcohol intakes in the past six months using two standardized and validated brief screening questionnaires: The Alcohol Use Disorders Identification Test (AUDIT) and the Alcohol Dependence Scale (ADS). The latter assesses alcohol withdrawal symptoms, impaired control over drinking, awareness of a compulsion to drink, increased tolerance to alcohol, and salience of drink-seeking behavior [29][30][31][32].
Alcohol consumption scores for each beverage were computed by: 1) transforming the responses to standardized alcohol units, and 2) averaging cross products of the quantity and frequency of beer, wine, and hard liquor.
Consumption phenotypes related to heavy drinking can be broken down into two categories: (1) consumption of 'too much too fast' and (2) consumption of 'too much too often' [29][30][31][32]. The first phenotype known as "heavy episodic drinking" was established a decade ago and was defined as five or more drinks in a day for males and four or more drinks in a day for females (i.e. the '5/4 definition'). The second phenotype refers to heavily drinking a higher quantity of alcohol than is recommended on a regular basis (i.e. 'too much too often'). Following the National Institute of Alcohol Abuse and Alcoholism guidelines criteria, men who reported >14, and women >7 drinks/week were classified into the hazardous drinkers group, while those who reported fewer drinks were included in the non-hazardous drinkers group [29][30][31][32]. Avariable was created to analyze drinking trajectories. The variable was the result of subtracting baseline total number of drinks consumed per week from the 6-month values. Based on individual trajectories, each participant was assigned to one of three mutually exclusive groups: 1) stable consumption; 2) increased consumption; and finally, 3) decreased consumption.

Cholesterol
Although for years it has been argued that the cholesterol content in the brain is fully independent of the systemic pool, there is increasing evidence to show the exchange of cholesterol between the brain and the blood pool [33]. Furthermore, data derived from animal models clearly demonstrates that a low, but continuous flux of sterol synthesis and export is required for normal CNS function. Given these findings and the limitations on obtaining direct CNS measures, we acquired fasting blood samples at baseline and at the 6 months follow-up. Total cholesterol (TC) levels were measured by routine enzymatic methods (KonePro, Konelab). In addition to treating mean TC values as a continuous variable, subjects were categorized as having or not having hypocholesterolemia. Hypocholesterolemia (levels <150 mg/dL) was defined according to the US National Cholesterol Education Program guidelines [34].

Plausible Confounding Factors
Information derived from income, education, age, race, and gender was considered in our study. These variables have been associated with drinking patterns previously. A family history of alcohol abuse was obtained as part of the initial assessments and was classified as yes/no. We also assessed family, partner, and/or friend support regarding HIV disease, treatment adherence, and alcohol related problems (ACTG, ADS). Due to the relationship between alcohol use and other drug risk behaviors, tobacco and drug use habits were assessed using standardized questionnaires.
Given the potentially confounding effects of health status on our outcome assessment, to measure and control for this effect in our analyses, we have assessed several general health status variables, such as anthropometrics, albumin and body mass index along with specific markers of HIV disease status. Weight was measured to the nearest 0.1 kg on a balance beam scale, with participants dressed in indoor clothing and without shoes. Height was measured to the nearest 0.1 cm, using a wall-mounted stadiometer. Body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. In addition, a detailed current and past medical history was obtained on each participant. Depression was assessed with the Beck Depression Inventory (BDI) form, a widely used measure with good internal consistency (coefficient alpha = 0.8), and adequate construct validity [31]. Each of the 21 items is rated on a 4-point Likert scale, ranging from 0 (neutral) to 3 (maximum severity), and summed to obtain a total score (0 to 63). Participants with BDI scores over 19 were classified as either having moderate to severe depression [35,36].
Using flow cytometry,as per the National Institute of Allergy and Infectious Diseases laboratory protocols, CD4 cell counts were assessed in freshly obtained whole blood. To assess the degree of HIV activity, viral load was assessed by an Amplicor HIV-1 monitor kit, using reverse transcriptase polymerase chain reaction (COBAS Amplicor Analyzer [Roche Molecular Diagnostics, Pleasanton, CA]).

Statistical Analyses
Data sets were analyzed using SAS 8.1 (SAS, Institute Inc., Cary, NC, USA) and SPSS 18 (SPSS, Inc., Chicago, IL, USA). Associations between the main variables of interest were examined with Pearson's correlation coefficient analyses. Subgroups were compared using analysis of variance.
Several approaches were used to investigate drinking trajectories.
The first analyses involved a paired Student's t test to compare changes from T1 to T2. The non-parametric Wilcoxon test was also used for these comparisons, as normality of the distributions could not be presumed in all of the cases. To prepare data for the second trajectory analysis, we subtracted baseline number of total drinks per week from those in the last visit, identifying three distinctive groups:1) those who increased alcohol consumption, 2) those who decreased consumption, and 3) those whose consumption levels remained stabilized.
Multivariate regression analysis was used to describe the associations between cholesterol and alcohol drinking trajectories, while controlling for all variables that were statistically significant in the univariate analyses. Multiple logistic regression analyses were used to evaluate the effects of cholesterol, and other potential risk factors on alcohol drinking. The initial model was constructed with age, socioeconomic status, body mass index, dementia and depression, as predictive variables. These factors were selected because they were significant (p=0.05) in the univariate analysis, or they were recognized risk factors (i.e. socio-demographics, global markers of health status, mood and cognitive disorders).
In all models, we adjusted for any baseline alcohol intake differences so as not to have this variable as a possible explanation of differences between trajectory groups in drinking. Nonsignificant variables were removed, beginning with the least significant variables, until the final full model was determined.
Regression output was reported as adjusted OR or RR when analyzing longitudinal data accompanied by 95% CI.
Additionally, we applied Bayesian Networks learning method to further understand interactions among the variables of interest.A Bayesian network is a probabilistic model that consists of two parts: a dependency structure and a probability model [37,38].
The probability model specifies how variables depend on others.
Usually, a variable only depends on a few other variables, called the parents. We evaluated (scored) a Bayesian Network using the following assumptions: (1) discrete variables, (2) Dirichlet prior parameter distributions, (3) multinomial likelihood functions; (4) parameter independence; (5) parameter modularity, and (6) OPEN ACCESS http://scidoc.org/IJHR.php no missing data [37,38]. A stage-wise approach was used to eliminate variables that ceased to be significant in the models. The probability model specifies the probability that a variable takes a certain value, given the value of its parents. Each statistical model assessed interactions between the significant variables and time.

Characteristics of the Study Population
MARCH was designed as a longitudinal, observational study to evaluate the impact of hazardous versus non-hazardous alcohol use on the health status of HIV-infected individuals receiving HAART therapy. With an overall participation rate of 98% and a retention rate of 85%, the sample included 125 participants who did not receive cholesterol-lowering medication, and who had their blood drawn at both the baseline and six-month follow-up periods. The mean TC level of the sample was 173 ± 43mg/dl (52-324mg/dl), with HypoCHL present in 40% of participants at baseline, and 33% at week 24. Only 5% had more than 250mg/ dl of TC. Cholesterol levels were unrelated to triglyceride levels and/or to malnutrition. In addition, we did not observe any differences in vitamin B-12 levels between hazardous and non-hazardous alcohol users, nor between subjects with low versus normal cholesterol levels (HypoCHL: 615 ± 394 vs. 674.2 ± 422). These findings are highly relevant because it indicates that the groups were similar, thus reducing the possibility that nutritional status/dietary habits may explain the observed differences on either cholesterol or neuropsychological functions. These results are also highly pertinent in light of studies indicating that poor learning and memory performance follow a detoxification predicted relapse [39]. Although evidence exists that poor health (e.g., malnutrition) is associated with both HAU and hypocholesterolemia, our analyses failed to uncover a significant association between hypocholesteromia and vitamin B-12 levels or malnutrition (see Table 1), suggesting that hypocholesterolemia was not the result of frailty. Furthermore, we did not find any differences in dietary intakes.
However, participants with HypoCHL had a 58% excess risk of having depressive symptoms (95% CI 1-3, p=0.05). Univariate analyses indicated that those with baseline depression were seven times more likely to increase the number of drinks/day, compared to those without any depressive symptoms (95% CI 1-10; p=0.05).

Cholesterol (CHL) Status and Alcohol Drinking Trajectories
Drinking was common in the sample, varying from 0-100 drinks per week ( Legend:Values are means ± SD or percentages. No significant differences in sociodemographic characteristics were found between groups except for race.

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http://scidoc.org/IJHR.php and those with normal CHL (18 ± 2 vs. 12 ± 1, p=0.015). Furthermore, participants with HypoCHL exhibited significantly higher AUDIT scores than their normal CHL counterparts (9.2 ± 0.7 vs. 7.3 ± 0.7, p=0.05). Notably, no differences in smoking rates were observed between cholesterol groups. Because the effects of advancing HIV disease might confound our results, we repeat the analyses in individuals with and without AIDS and found no differences in the results.
Surprisingly, at the follow-up evaluation after 6 months post-HAART, most drinkers had significantly reduced the total number of alcohol drinks/week (from 32.9 drinks/week to 15.5, p=0.005) and maximum number of drinks consumed on any one occasion (16.8 max. to 12.5). Therefore, we performed a trajectory analysis and were able to identify three distinctive trajectories regardless of initial drinking levels. Overall, a significant subgroup (40%) either decreased or quit drinking, 15% maintained a constant level, and the remaining sample (45%) increased drinking throughout the duration of the study.
As illustrated in Figure 2, the drinking trajectories clearly differed between those having hypocholesterolemia versus normal values at baseline. Compared to those with normal baseline CHL, HypoCHL participants were more likely to increase their alcohol consumption during the course of the study (RR=2.7, 95% CI 1-7.9, p=0.045).
As depicted in Table 2, among hazardous drinkers, a significant decline in all drinking measures was observed in the subgroup with normal cholesterol values. In contrast, significant increases over the six months were observed in those with hypocholesterolemia. Further strengthening our findings was the evidence of a doserelationship between baseline cholesterol values and risk of higher alcohol consumption at the last visit (see Table 3).

Final Analyses
In the final controlled model (i.e. age, race, baseline drinking, CD4) three important results emerged. First, analyses identified among several potential predictors five significant risk factors predicting increases in alcohol use: being a smoker (OR= 2.9 95% CI 1.-6.4, p=0.008), being a liquor user (OR= 1.9 95% CI 1-4, p=0.05), having HypoCHL (OR= 1.5 95% CI 1-2.8, p=0.04), a close relative with hazardous alcohol use (OR=3, 95% CI 1.8-5.6; p=0.0001), and/or having limited family support. Second, the three-way interaction of cholesterol, depression and smoking was not significant indicating that hypocholesterolemia is an independent risk factor of drinking. Third, the model also reveals that neither depression nor CD4 counts or stage of HIV disease (i.e., symptomatic, asymptomatic or AIDS) significantly alter the probability of increased alcohol use.
To further establish if hypocholesterolemia affected drinking behavior and to determine the variance HypoCHL contributed to predicting alcohol use variables beyond all the other variables in the study, we performed a Bayesian modeling analyses. This modeling data confirms a relationship between baseline cholesterol and subsequent alcohol intake, (i.e., number of days drinking and in number of drinks/day). Figures 3 and 4 show probabilities associated with the structures when cholesterol is manipulated. This manipulation enabled us to quantify the dynamics of all other variables. For example, the model estimated an 87% probability of either maintaining or increasing alcohol intake during the study, in the event that baseline cholesterol was low (choles=State1). In this scenario, only 14% of the participants exhibited reduced alcohol intakes. Noteworthy, when we modeled cholesterol levels to be normal (choles=State0), as much as 41% of the participants were estimated to reduce their alcohol intakes. According to the model, if those with hypocholesterolemia smoked tobacco, only 11% would be able to quit drinking.

Discussion
This study provides what appears to be the first evidence in the scientific literature, indicating that hypocholesterolemia may indeed affect drinking behaviors in PLWH. Overall, the considered risk factors explained about 87% of the attributable risks of maintaining or increasing alcohol intake in PLWH. Over a 6-month follow-up period, an increase in alcohol intake was related to several factors, including smoking, mental health (including depressive symptoms), age, and race. While it is widely acknowledged that alcohol reduces cholesterol levels, both in   This modeling data confirms a relationship between baseline cholesterol (choles 0= normal vscholes 1= <150) and alcohol intake, i.e., number of drinks in a day (drinksda) and difference in number of drinks/day between baseline and final visit (diffdd state 2= reduced; state 1= maintain and state 0=increased).
OPEN ACCESS http://scidoc.org/IJHR.php plasma as well as in the brain [40,41], the possibility that this relationship is bi-directional has been largely ignored. Although our study design precludes casual inferences, several factors may boost confidence in our findings. First, our model explained a significant proportion of the alcohol trajectories, making it less likely that hypocholesterolemia was merely a moderator of our findings. Second, we performed detailed dose-response and temporal analyses, where baseline values were able to predict results 24 weeks later, thereby strengthening our results. Third, there was a robust and persistent relationship after controlling for confounders supports the notion that they are closely related. Importantly, we controlled for key characteristics common among those who drink alcohol, including sociodemographic (i.e., age, education), psychiatric (i.e., depression), and other behavioral factors (i.e., tobacco). Fourth, while the literature on cholesterol in substance abusers is sparse, Buydens-Branchey and Branchey made a similar observation in cocaine addicts [42]. According to their analyses patients, with HypoCHL at time of admission were more likely to relapse at all-time points (3, 6, and 12 months after discharge).
OPEN ACCESS http://scidoc.org/IJHR.php and postsynaptic serotonin function. These alterations could lead to cognitive impairment, mood disorders and diminished impulse control, all neurobiological features of HAU [11][12][13]51]. Cholesterol interactions with dopamine have also been well documented in animal models, and given their critical role in alcohol behaviors, our results are not uncommon or unexpected. [52].
These findings pose several clinical and public health implications.
First, our Bayesian model highlights the need to address cholesterol and smoking problems simultaneously to produce an impact on hazardous alcohol use. Interventions can be approached from a dietary standpoint with cholesterol supplements that can be incorporated along with pharmacological or behavioral treatments. The findings also reinforce the notions that even a short educational session, such as the one we provided when informing the participants about the study, can potentially yield considerable results.
However, some limitations in our study should be noted. First, no experimental manipulation was conducted, so our results, while they may be compelling, provide only associations and do not indicate any degrees of causality. While our longitudinal design reinforces the relevance of our results, the short-followup periods limit our capacity to draw stronger, more well-defined conclusions. Findings are strictly limited to the study site, and given the strict exclusion criteria, our study can be criticized from the standpoint of its representativeness. However, these restrictions allowed examination of the potential effects of cholesterol on drinking, though they precluded examination of the potential indirect and interactive effects that might be produced in the presence of such disorders. Finally, we are aware that the study could have been executed in the general population, thus avoiding the confounding effects of HIV disease. However, the striking prevalence of both HAU and hypoCHL among PLWH was the decisive factor, which essentially drove our selection.
The findings of our study should add confidence in the longterm value and applicability of these relationships to the research arena. At best, our analyses open the scientific gateway for studies with longer follow-ups to replicate our findings and to unravel distinct mechanisms underlying the relationship between hypocholesterolemia and alcohol. Considering the dramatic increases in lipid lowering medication prescriptions among PLWH, and the high prevalence of alcohol use disorders [4][5][6][7][8], these results also emphasize the need for further studies to elaborate the risks and benefits of marked cholesterol reductions in this population group.