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Toxicological and Metabolic Response of the Bollworm Heliothis Zea (Boddie) to Ddt.

A study of the toxicological and metabolic response of the bollworm larva, Heliothis zea (Boddie), to DDT was conducted over a three-year period. The primary objectives of this investigation were to determine if resistance to DDT had developed and to elucidate the metabolic fate of DDT in this species. Cultures of bollworm larvae from three selected locations in Louisiana were established for this study. Larvae from Baton Rouge, where populations are seldom exposed to DDT, were compared tp those from the Bunkie and Waterproof areas where DDT has been used extensively since 1947. The response to DDT of bollworm larvae from other areas of Louisiana was also investigated. In addition a survey was made of the response of bollworm larvae to endrin from five areas and toxaphene-DDT (2-1) mixture from three areas in Louisiana. Cultures were established by collecting adults from cotton fields by means of a light trap. Toxicological information was obtained by applying scalar doses of the insecticide to the dorsal thoracic region of individual larvae. LD-50's were determined by means of probit analysis of the 72-hour post-treatment observations. The response of second instar bollworm larvae from all areas to DDT was approximately the same. However, the slopes of the