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Recent advances in electron immunocytochemistry.

Considerable progress has been made in the development of methods for the localisation of antigens at the ultrastructural level using both transmission and scanning electron microscopy. There is currently no technique which is appropriate for all antigens, although, almost without exception, colloidal gold-linked reagents provide the electron-dense marker. For post-embedding techniques, several approaches are available all utilising low temperatures during the preparative stages. Cryosectioning and embedding by the PLT method both require the antigen to withstand some degree of fixation. For fixation-sensitive antigens, freeze substitution in solvents lacking fixative agents is an approach offering considerable potential.