{'fraction_non_alphanumeric': 0.025952960259529603, 'fraction_numerical': 0.0202757502027575, 'mean_word_length': 4.904306220095694, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
[Detection of positive-strand of transfusion transmitted virus fragment in the liver of cryptogenic hepatitis].

OBJECTIVE
To evaluate whether transfusion transmitted virus (TTV) replicates in the liver and to analyze the relationship between TTV and cryptogenic hepatitis.


METHODS
A 3.2 kb TTV fragment was detected by nested polymerase chain reaction (PCR) in 31 serum samples of patients with cryptogenic hepatitis who came from a school where was in an outbreak area of cryptogenic hepatitis during 1996 and in 30 healthy individuals. A hybridization/nuclease protection assay was used to detect positive-strand TTV fragment from the liver specimens of the 7 patients.


RESULTS
TTV DNA was detected in serum samples from 30 of 31 (96.9%) patients and from 18 of 30 (60%) healthy individuals, respectively. There was significant difference in the prevalence of TTV infection between the two groups. Based on hybridization/ nuclease protection assays, we detected positive-strand TTV fragment in all of 7 liver specimens of the patients.


CONCLUSIONS
TTV is related possibly to cryptogenic hepatitis. TTV can replicate in the liver. Although TTV only causes mild liver damage, a few patients will suffer from chronic hepatitis.