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Ultrastructure and DNA Sequence Analysis of Single Fossil Sediment Cells

Abstract The method we have developed allows the cytoplasm of a single fossil cell to be sufficiently preserved as to be distinguished by fluorescence microscopy. Analysis of fossil DNA involved PCR amplification of the spacer region between rrn5 and trnR genes in the chloroplast genome. The DNA and consensus sequences, obtained from the same PCR amplification fragment, were analysed by BLAST search and CLUSTAL W multiple alignment software.