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Can minor bcr/abl translocation in acute leukemia be discriminated from major bcr/abl by extra-signal FISH analysis?

We investigated whether bcr/abl extra signal(ES)-probe can discriminate minor-bcr (m-bcr) and major-bcr (M-bcr). The number of fusion signals was two in m-bcr and one in M-bcr with ES-fluorescent in situ hybridization (FISH), while with double-FISH, there were two fusion signals in both m-bcr and M-bcr. It was possible to discriminate them by ES-FISH, whilst it was impossible by double-FISH.