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Amplification and detection of $i(mycoplasma pneumoniae)

Amplification primers and methods for specific amplification and detection of an ORF6 gene are disclosed. The primer-target binding sequences are useful for amplification and detection of Mycoplasma pneumoniae target in a variety of amplification and detection reactions. Figure (I) illustrates detection of a M. pneumoniea nucleic acid ORF6 gene target sequence in a strand displacement amplification (SDA) reaction.