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Evaluation of some morphological characteristics of spores of two species of microsporida by scanning electron microscope and freeze‐etching techniques

Scanning electron microscopy revealed spores of Nosema apis and Thelohania fibrata to be egg‐shaped, but only the mature spore of T. fibrata was shown to possess a horseshoe‐like concavity at the posterior pole. Freezeetched preparations indicated that this concavity was due to a thin area of the spore coat. Freeze‐etching studies also show spores of N. apis do possess an umbrella‐shaped polaroplast, and a polar filament which is arranged in a double layer with over 30 coils. The spore of T. fibrata contains a pear‐shaped arrangement of the polaroplast membrane, and a polar filament arranged in a single layer of 22 coils.