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Effect of stobadine and vitamin E on the ischemic reperfused brain tissue.

The aim of this work was to verify the protective effect of stobadine, a drug with pyridoindole structure [1] on brain ischemia and reperfusion. We induced the brain ischemia and reperfusion by ligation of both carotid arteries for 4 h, reperfusion lasted 1 h. The extent of lipid peroxidation in the brain cortex was estimated by a thiobarbituric acid (TBA) test. It is known that ischemia and reperfusion of brain tissue is accompanied. Thus in out present work we investigated besides lipid peroxidation also the content of thiol groups after reaction with dithio-bis-nitrobenzoic acid (DTNB). The effect of vitamin E, a lipid antioxidant and drug able to maintain the cellular protein thiols was compared with the effect of 1