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REDUCED ACTIVITIES OF DIVALENT CATION ACTIVATED ATP-ASE AND 5′ -NUCLEOTIDASE IN GLYCEROL INDUCED ACUTE RENAL FAILURE

Reduced renal blood flow and glomerular filtration rate are the common findings in mioglobinuric acute renal failure (ARF). On the other side, adenosine plays an important role in regulation of renal blood flow, glomerular filtration rate and activity of 50-nucleotidase. Ecto-ATPases (Ca2þ and Mg2þ) hydrolyzing extracellular nucleotide tri-and/or diphosphates with 50-nucleotidase seem to represent the major source of extracellular adenosine in the kidney (1). Experimentally glycerol-induced ARF is also followed by generation of reactive oxygen species, especially with release of hydrogen peroxide (2). Phenolic plant flavonoid – quercetin (3,30,40,5,7-pentahydroxyflavon) has been established as a strong antioxidative agent that have protective effects on kidney in ischemic renal injury (3) and hydrogen peroxide induced apoptosis (4). However, it is well known, that quercetine inhibit activity of many enzymes including ATPases. The present study was undertaken to evaluate changes of divalent cation (Ca2þ and Mg2þ) activated ATPase and 50-nucleotidase during the course of experimentally glycerol-induced ARF. At the same time, protective effects of quercetine were tested. Acute renal failure was induced in male Sprague–Dawley rats (weighing 200–280 g.) with injection of glycerol (8mL/kg 50% solution) in hindlimb