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Seed Morphology in Species from the Silene mollissima Aggregate (Caryophyllaceae) by Comparison with Geometric Models

The description of seed shape by comparison with geometric models allows shape quantification, providing the means for an accurate comparison between different species or populations. Geometric models described for the lateral and dorsal views of the seeds of Silene species are applied to the quantification of the shape in the seeds belonging to twenty populations of the eleven taxa of S. mollissima aggregate. Cardioid models LM1, LM5 and LM6 adjust differentially to the lateral views of the seeds, while models DM1, DM5 and DM6 are applied to the dorsal views of the seeds. Quantification of the lateral view of seeds with LM5 results in two groups of species of different geographic origin. The seeds more resembling DM5 include S. andryalifolia, S. badaroi, S. gazulensis, S. hifacensis and S. tomentosa, i.e., the taxa with a continental distribution from southern Spain to northern Italy; in contrast, the group of seeds with lower similarity to DM5 includes those from species in northern Africa and the Mediterranean Tyrrhenian islands: S. auricolifolia, S. hicesiae, S. ichnusae, S. mollissima, S. oenotriae and S. velutina. The description of the seed shape based on geometric models contributes to investigating the relationships between related species and constitutes a promising technique for taxonomy.


Introduction
The genus Silene L., with more than 700 species, includes an important proportion of the diversity found in the family Caryophyllaceae Juss. The taxonomy of this genus was traditionally based on morphological criteria, and more recently has received the support of intensive DNA sequence analysis [1]. The genus was divided in three subgenera: Lychnis, Silene and Behenantha. The subg. Silene contains 11 sections, including Sect. Siphonomorpha Otth, a large group comprising about 150 species of perennial diploids [2], including both the S. italica and S. mollissima aggregates [3][4][5].
The S. mollissima aggregate contains 11 species found in different biogeographic provinces along the western Mediterranean Basin [6,7]. Among them, five species are endemic to the Tyrrhenian islands macro-hotspot (sensu Cañadas et al. 2014 [8]); these are: Alonso and S. tomentosa Otth. These species are currently identified by the morphological characters of their epigean vegetative parts (leaves, hairs), as well as their inflorescence, flowers and capsules [4,9].
The 11 species of the S. mollissima aggregate that are the subject of this work constitute a paradigm for the study of plant diversity in the Mediterranean area, a region characterized by high rates of endemism due to isolation, either in islands, through mountain ranges or due to geographic micro-environments [10]. Thus, S. tomentosa is endemic to Gibraltar, a small area with very specific characteristics [11], while S. gazulensis is endemic to the Sierra de los Gazules in the surroundings of Gibraltar [12]. S. andryalifolia, related to these species, has a broader distribution. The two Ibero-Levantine taxa, S. hifacensis and S. mollissima, are not sympatric. S. hifacensis only occurs on Ibiza and Alicante, while S. mollissima occurs on the Gymnesian islands of the Balearic Archipelago (Mallorca, Menorca and Cabrera) but not on the Pityusic islands (Ibiza or Formentera). Five of these species (S. badaroi, S. ichnusae, S. hicesiae, S. oenotriae and S. velutina) have a distribution centred in the Tyrrhenian area, with S. badaroi broadly distributed in the Provence and coast of north-western Italy; S. velutina is endemic to Sardinia and Corsica, and S. ichnusae is limited to north-western Sardinia while S. hicesiae and S. oenotriae occur in the southern Tyrrhenian sea. All these species are included in the section Syphonomorpha of subgen. Silene [1]. In this work, we have presented a morphological description of 20 populations from these species conserved in the Sardinian Germplasm Bank (BG-SAR; [13]) in order to find affinities between them as well as to investigate the variation in shape between species and populations.
Variations in the size and shape of the seeds represent an important source of information of high potential in taxonomy, particularly in Silene [14][15][16][17][18][19]. The application of image analysis techniques allows to obtain measurements related to the color, size and shape of seeds and to distinguish between different taxa or populations [20][21][22][23][24][25].
Based on previous studies on the seed morphology of the model plants Arabidopsis thaliana (L.) Heynh. [26,27], Lotus japonicus (Regel) K. Larsen and Medicago truncatula Gaertn. [28,29], seed shape descriptions based on the comparison with geometrical models have been applied to diverse plant genera and families [30][31][32], where the J index represents the percent of similarity between the geometric figure used as a model (cardioid) and the seed image. A striking similarity of the seed shape with the cardioid has been reported for the Silene species, in particular within Silene subg. Behenantha, with values of J index superior to 90 in a number of species (e.g., S. noctiflora L., S. conica L. and S. latifolia Poir.) [33]. In addition to the cardioid (Model 1, or LM1, where LM stands for lateral model), other models have already been described for the lateral views of Silene seeds [34,35]. LM2 is a flattened cardioid that demonstrated maximum similarity with the species S. noctiflora (J index 94.4) and S. latifolia (J index 93.0); LM3 was defined as an open cardioid curve having maximum similarity with S. gallica L. (J index 90.4) and LM4 was a flattened and elongated cardioid curve that indicated maximum similarity scores with S. latifolia (J index 92.5) and S. diclinis (J index 91.5) [33]. LM5 and LM6 are modified forms from model 3 with variations in the hilum region. Values of J index superior to 90 were obtained in S. diversifolia Otth and S. tridentata Ramond ex DC. [34]. Models LM7 and LM8 present other morphological peculiarities and provide J index values superior to 90 with S. portensis L. (both models), S. nicaeensis All., S. littorea Brot., S. portensis and S. scabriflora Brot. (model LM8) [34]. Models for the dorsal views of Silene seeds have also been described [35]. The J index values were more stable than the other typical morphological measures (i.e., area, length and width); hence, the association between the seed morphology and geometrical figures was stated as robust and could be used for classification purposes [33][34][35].
Concerning the seed morphology of the S. mollissima aggregate, morpho-colorimetric image analysis methods have been applied, revealing differences between the species [14,36]. By studying the seed morpho-colorimetric characteristics of S. badaroi, S. ichnusae, S. hicesiae, S. oenotriae and S. velutina, Murru et al. [36] reported that the current systematic treatment at the species level for these taxa is confirmed. However, the authors highlighted the need of further investigations regarding the taxonomic position of S. hicesiae in the whole aggregate as well as regarding the differentiation of S. ichnusae from S. velutina [36]. Nevertheless, the results from automated image analysis reported so far do not discriminate between the differences in size and shape, and the differences resulting from the analysis may be due to a mixture of shape, size or even color characteristics, while we are interested by the identification of differences in seed shape. The seeds of a given species or population can have a conserved shape that corresponds to a geometric model. This is detected by values of J index superior to 90 and has been reported in populations of diverse species of Silene [33][34][35].
Previous studies on the seeds' characteristics in the taxa of a specific group membership proved that this type of analysis can be a useful tool for discriminating the species [23,24,29,35,36] or also in separating the taxa in different groups, such as, for example, the coastal species from the mountain ones [25]. These analyses are nondestructive, repeatable and, by incorporating the seed trait information with the leaves, hairs, flowers, fruit analysis or other morphological characteristics of a plant, may provide an important contribution for taxonomic studies.
Our objectives in this work are the following: (1) to describe the geometric models that define the seed shape in the species of Silene mollissima aggregate, and (2) to identify differences between the species in the shape of their seeds. We hypothesize that the new information provided by the geometric models analysis of seed shape may confer new knowledge useful to understand the relationships among species and to provide a new basis for the next taxonomic investigations regarding Silene taxa. Table 1 presents a summary of the mean values and standard deviations for area (A), perimeter (P), length (L), width (W), aspect ratio (AR), circularity (C) and roundness (R) corresponding to the lateral views of the seeds in the 11 species of S. mollissima aggregate. Differences between the species were found in the size and shape for all the measurements. The mean area values were between 0.86 (S. badaroi) and 1.50 mm 2 (S. andryalifolia). Three groups of different sizes were defined. The largest seeds corresponded to S andryalifolia, S. auricolifolia and S. velutina, the intermediate seeds to S. hifacensis, S. ichnusae, S. mollissima, S. oenotriae and S. tomentosa and the smallest seeds to S. badaroi, S. gazulensis and S. hicesiae.

Lateral Views of Seeds
The aspect ratio was between 1.15 in S. auricolifolia and 1.33 in S. velutina; three groups were defined corresponding to the rounded seeds with the smallest values of aspect ratio (S. auricolifolia), seeds with intermediate values (S. andryalifolia, S. badaroi, S. gazulensis, S. hicesiae, S. hifacensis, S. ichnusae, S. mollissima and S. tomentosa) and elongated seeds (S. hicesiae, S. oenotriae and S. velutina). The coefficient of variation had higher values in the measurements of size (A, P, L and W) than in the shape measurements (AR, C and R).
Different populations were analyzed in four species: S. badaroi, S. hicesiae, S. hifacensis and S. velutina. In the species with two populations analyzed (S. hicesiae and S. velutina), there was one population with larger seeds and the other with smaller seeds (not shown). There was no difference in aspect ratio, circularity or roundness in any of these two cases. There were also differences in seed size among the populations of S. badaroi (three populations) and S. hifacensis (six populations) (not shown). Differences in circularity were found between the populations of S. badaroi (not shown). In S. hifacensis, there were also differences in size, aspect ratio, circularity and roundness between the six populations studied (not shown). Table 2 presents a summary of the mean values and standard deviations for A, P, L, W, AR, C and R corresponding to the dorsal views of the seeds in the 11 species of S. mollissima aggregate. Differences between the species were found in size and shape for all the measurements. The mean area values were between 0.71 (S. hicesiae) and 1.51 mm 2 (S. auricolifolia). Five groups of different sizes were defined. The largest seeds corresponded to S. auricolifolia, followed by the seeds of S. andryalifolia. The seeds of S. oenotriae and S. velutina were of intermediate size. S. hifacensis, S. ichnusae, S. mollissima and S. tomentosa were of small-intermediate size. The smallest seeds corresponded to S. badaroi, S. gazulensis and S. hicesiae. Table 1. Results of Kruskal-Wallis and post hoc tests for the size and shape measurements in the lateral views of seeds in species of the Silene mollissima aggregate. Mean values and coefficient of variation (given in parentheses) are indicated for area (A), perimeter (P), length (L), width (W), aspect ratio (AR), circularity (C) and roundness (R). Values marked with the same superscript letter in each column correspond to populations that do not differ significantly at p < 0.05. N indicates the number of seeds analyzed. Similar to the lateral view, the aspect ratio was between 1.08 in S. auricolifolia and 1.78 in S. velutina. Four groups were defined by the seeds with large (S. gazulensis, S. hicesiae, S. hifacensis, S. mollissima and S. velutina), intermediate (S. badaroi and S. ichnusae), intermediate-small (S. andryalifolia, S. oenotriae and S. tomentosa) and smallest values of aspect ratio (S. auricolifolia). The coefficient of variation had higher values in the measurements of size (A, P, L and W) than in the shape measurements (AR, C and R).

Dorsal View of Seeds
In the species with two populations analyzed (S. hicesiae and S. velutina), there was no difference in size, aspect ratio, circularity or roundness between the populations (not shown). In contrast, there was a difference in the size and aspect ratio among the populations of S. badaroi (three populations) and S. hifacensis (six populations) (not shown).

Lateral View of Seeds
The average silhouettes obtained for the lateral view of the seeds in all 20 populations belonging to 11 species are shown in Figure 1. They were compared with the models LM1 to LM8 described in Refs. [33,34]. Table 2. Results of Kruskal-Wallis and post hoc tests for the size and shape measurements of the seeds in species of the Silene mollissima aggregate. Mean values and coefficient of variation (given in parentheses) are indicated for area (A), perimeter (P), length (L), width (W), aspect ratio (AR), circularity (C) and roundness (R). Values marked with the same superscript letter in each column correspond to populations that do not differ significantly at p < 0.05. N indicates the number of seeds analyzed.  The average silhouette of each population was compared with the eight geometric models. The results of the comparison are given in Table 3. The values of J index (percent similarity to the models) superior to 90 were obtained in all the species tested. Maximum values were obtained with model LM1 for S. andryalifolia, S. auricolifolia and S. ichnusae, with model LM4 for S. badaroi, S. hicesiae, S. hifacensis and S. oenotriae and with model LM5 for S. gazulensis, S. mollissima and S. tomentosa. S. velutina showed the best score with model LM8. The J index was calculated with models LM1, LM4 and LM5 for all the species.

Dorsal View of Seeds
The average silhouettes obtained for the dorsal view of the seeds in all 20 populations belonging to 11 species are shown in Figure 2. They were compared with models DM1, DM5 and DM6 [35]. The results of the comparison of the average silhouette of each species in the dorsal views of the seeds with the three geometric models tested are given in Table 4. Values superior to 90 were obtained for all the species except for S. auricolifolia, whose seeds are round in their lateral view. Maximum values were obtained with model DM5 for S. andryalifolia, S. badaroi, S. oenotriae and S. tomentosa. With model DM6, the highest values were obtained for S. gazulensis, S. hicesiae, S. hifacensis, S. ichnusae, S. mollissima and S. velutina. The J index values were tested with models DM1, DM5 and DM6 for the seeds of all the species. The percent similarity (J index) of the seeds with the models giving the highest values in the comparison with the average silhouettes (LM1, LM4 and LM5) was obtained for all the species (Table 5).    The coefficient of variation had lower values in the measurements of J index than in the other shape measurements (AR, C and R; see Table 1).

Shape Quantification in the Dorsal Views with Models DM1, DM5 and DM6
The percent similarity of the dorsal views of the seeds with models DM1, DM5 and DM6 (J index) was obtained for all the species (Table 6). Table 6. Results of Kruskal-Wallis and post hoc tests for the J index values (percent of similarity of the dorsal views of seeds with models DM1, DM5 and DM6) of the 11 species of the Silene mollissima aggregate. Mean values and coefficient of variation (given in parentheses) are indicated for J index values with the different models. Values marked with the same superscript letter in each column correspond to populations that do not differ significantly at p < 0.05. N indicates the number of seeds analyzed. In bold, upper values obtained for each species.   Table 2).

Discussion
The description of the seed shape by comparison with geometric models allows the quantification of shape, an important step for phenotype characterization in the analysis of differences between varieties and species as well as for the study of the effect of environmental factors. The information reported in this study allows to increase the knowledge on the seed traits of species of Silene [15,16,18,33,34], with particular reference to the members of Silene mollissima aggregate [36]. The overall seed shape in the lateral views of the Silene species resembles a cardioid and figures related to it, while, in the dorsal views, it is more elongated, resulting in shapes related to modified ellipses and hyper-ellipses. The models used in this work for the lateral views of the seeds (LM1 to LM8) were described by Martín-Gómez et al. and Juan et al. [33,34], while the models DM1, DM5 and DM6, used in the description of the dorsal views of the seeds, were recently described by Rodriguez-Lorenzo et al. [35]. This is the fourth publication reporting seed shape quantification in species of Silene by this method and the first time that a set of lateral and dorsal models has been applied simultaneously to a set of geographically and phylogenetically related species.
The first conclusion of this work confirms the previous observations detected by using this type of analysis (e.g., [33][34][35]) and especially refers to the stability of shape. In summary, (I) the coefficient of variation is smaller in shape than in size measurements, and (II) the coefficient of variation is smaller in a measurement that describes the overall similarity of a seed image with a geometric figure (J index) than in general shape measurements (aspect ratio, circularity or roundness). In this work, differences in the size between populations were found in S. badaroi, S. hicesiae, S. hifacensis and S. velutina, while differences in the shape estimated as J index values with the different models were found only in the comparisons made with average silhouettes.
The differences in the size between the populations of the same species could not be correlated with known geographical factors. The populations having different seed size of species S. badaroi, S. hicesiae, S. hifacensis and S. velutina were located close to each other to attribute differences to climatic features, such as mean minimum temperatures or latitude. In the other cases reported, the differences in seed size in S. dioica were attributed to local, micro-environmental effects acting on a group of plants [37], similar to those described in Arabidopsis helleri (L.) O'Kane & Al-Shehbaz due to the presence of metals in the soil [38]. Differences in size between populations can also be attributed to the date of collection; for example, the seeds produced earlier in a plant are larger than seeds produced later [39].
A linear discriminant analysis (LDA) based on morpho-colorimetric measurements was applied to five Tyrrhenian species of the S. mollissima aggregate [36]. In this work, S. badaroi and S. hicesiae were separated from each other and from the other species analyzed (S. ichnusae, S. oenotriae and S. velutina). There are multiple factors influencing the result of LDA, while our analysis addresses more precisely the question of overall seed shape differences between species or populations independent of other considerations of size or color measurements.
The 11 species from the S. mollissima aggregate can be included in two groups for the lateral views and three for the dorsal views of seeds. Concerning the lateral views, the first group showing higher values of J index with model LM5 includes S. andryalifolia, S. badaroi, S. gazulensis, S. hifacensis and S. tomentosa; the second group, with lower values of J index with LM5, includes S. auricolifolia, S. hicesiae, S. ichnusae, S. mollissima, S. oenotriae and S. velutina. S. gazulensis is endemic in the Gibraltar area and its relatedness with S. tomentosa was already reported in the description of the former species [35].
In relation to the dorsal views, three groups resembling the results of the analysis of the aspect ratio were obtained. First, S. auricolifolia, with rounded seeds, forms an independent group. The second group contains all the species whose seeds have intermediate values of aspect ratio and whose dorsal views resemble model DM5 (S. andryalifolia, S. badaroi, S. oenotriae and S. tomentosa) and the third group, similar to species with higher values of aspect ratio, contains seeds resembling in their dorsal view the model DM6 (S. gazulensis, S. hicesiae, S. hifacensis, S. ichnusae, S. mollissima and S. velutina). According to their lateral and dorsal shape, and in relation to the other species studied by this method, the species of the S. mollissima aggregate resemble S. conica and S. coutinhoi, whose dorsal views adjust well to DM5 and DM6, respectively [35]. These two models have similar figures, with the only difference being that DM6 is narrower than DM5. The Iberian species S. coutinhoi is closely related to these species in the Silene sect. Siphonomorpha [40].
The analysis carried out in this work permitted to describe the seed shape of 20 populations of 11 taxa of S. mollissima aggregate as well as to detect two groups for the lateral views and three groups for the dorsal views of seeds of species of different geographic origin. The description of the seed shape by comparison with geometric models should be used as a complementary method in studies that take into consideration other morphological characteristics, such as the morpho-colorimetric quantitative and qualitative features of seeds, leaves, hairs, flowers, fruit or other characteristics of a plant, in order to ensure a more effective performance of classification among taxa. It is a low-cost and non-invasive approach, may contribute to the future studies on the other species of Silene and could be useful as the basis for a large-scale study.

Seeds of Silene Analyzed
Seeds of the 11 species of the Silene mollissima aggregate were obtained in the Sardinian Germplasm Bank (BG-SAR; [40]) and are described in Table 7. The localities of origin of the seed populations used in this work are shown in Figure 3. The accessions of each species were stored at −25 • C and preserved on the basis of established international protocols [41,42].

Seed Images
Photographs were taken with a Nikon Z6 camera with an objective AF-S Micro NIKKOR 60 mm f/2.8G ED. From an initial photograph made to the pool of seeds in each population and containing between 30 and 110 seeds, composed images containing 20-30 seeds regularly oriented per accession were prepared with Corel Photo Paint. In these images, the seeds were aligned to allow further analysis in ImageJ. The images are stored at: https://zenodo.org/record/6205212#.YhOpUujMKM8 (accessed on 21 February 2022).

General Morphological Description
Area (A), perimeter (P), length of the major axis (L), width (W), aspect ratio (AR is equal to L/W), circularity (C) and roundness (R) were obtained for the lateral views of seeds of each species from the initial photographs containing 16-110 seeds with ImageJ program [43]. The seeds were oriented with the micropyle to the right. A ruler was the reference for the conversion of pixel units to length or surface units (mm or mm 2 ). The circularity index and roundness were calculated as described [44]. Circularity is the ratio (4π × A)/P2, while roundness is (4 × A)/πL2; in consequence, circularity decreases with irregularities of seed surface that increase the perimeter, but roundness is not affected.

Obtention of an Average Silhouette
The average silhouette is a representative image of seed shape for each group of seeds. A total of 20 seeds were used for each studied species. The silhouette was obtained in Corel Photo Paint by the protocol described [45] (a detailed video is available at Zenodo: https://zenodo.org/record/4478344#.YBPOguhKiM8, accessed on 2 September 2021). The layers containing the seeds are superimposed and the opacity is given a value of 20 in all layers. All the layers are combined, and the brightness is adjusted to a minimum value. From this image, we are interested in the inner region representing the area where most of the seeds coincide, which is the darkest area. To select it, we use the magic wand tool and, with tolerance equal to 10, this selection is copied and pasted as a new layer.

Geometric Models Used in the Comparisons
The models used in this work were: for the lateral views of seeds, the eight models already described for Silene seeds [33,34]   where S is the area shared between the seed image and the model and T, the total area occupied by both figures. J index ranges between 0 and 100, reaching maximum values when the geometric model and the seed image areas coincide. A high value of J index, i.e., high similarity with a given model, means a precise definition of seed shape for a particular species. A good adjustment to the model was considered when J index values were superior to 90 [33].
J index was calculated with the eight geometric models described for Silene on the average silhouettes of each species. Those models giving high values were applied to calculations of J index with the samples of 20-30 seeds.
Area calculation was carried out by superimposing the model on each seed image, searching a maximum adjustment between the shapes of the seeds and the geometric model ( Figure 4). Three files were kept for each composition: (1) a document (PSD format) with the seeds and the geometric figure adapted to each of them, in which it is possible to make corrections; (2) a file (JPG format) with the geometric models in black, which is useful to obtain total area (T) with the software ImageJ and (3) another file in JPG format with the geometric models in white, useful to obtain the values of area shared between the geometric figure and the seed image (S) in ImageJ (Figure 4). Image composition with seeds and models was done in Corel PHOTO-PAINT X7, and area quantification in ImageJ. Figure 4 illustrates examples of the adjustment between seed images and the geometric models with indication of the areas measured for the calculation of the J index.

Statistical Analysis
The raw data are available at: https://zenodo.org/record/6378816#.YjrjbOfMKM8 (accessed on 23 March 2022). The mean, minimum and maximum values and the standard deviation were obtained for all the measurements indicated (A, P, L, W, AR, C and R) as well as for J index with the different models. Statistics were analyzed on IBM SPSS statistics v28 (SPSS 2021) and R. software v. 4.1.2 [46]. As some of the populations did not follow a normal distribution, non-parametric tests were applied for the comparison of populations. Kruskal-Wallis test was completed followed by stepwise stepdown comparisons by the ad hoc procedure developed by Campbell and Skillings [47]. P values inferior to 0.05 were considered significant. The coefficient of variation was calculated as CVtrait = standard deviationtrait/meantrait × 100 [48].

Conclusions
The seed morphology in the 11 species of the S. mollissima aggregate has been analyzed based on the comparison with geometric models both in the lateral and dorsal views. In the lateral view, the seeds resemble a cardioid (LM1) or models derived from it (LM4 and LM5). The comparison with LM5 revealed two groups of high and low similarity with this model. The group of high similarity to LM5 contains the species from Andalousie and Levant in southern Spain and the region of the Mediterranean coast in France and northern Italy with S. andryalifolia, S. badaroi, S. gazulensis, S. hifacensis and S. tomentosa. The group of lower similarity to LM5 includes the species of the islands along the Mediterranean Sea and north Africa: S. auricolifolia, S. hicesiae, S. ichnusae, S. mollissima, S. oenotriae and S. velutina. S. auricolifolia constitutes a separate group due to roundness in both the lateral and dorsal views.
In comparison with the other species analyzed by geometric models, and in particular in their dorsal views, the seeds of the S. mollissima aggregate, excluding S. auricolifolia, resemble species S. conica and S. coutinhoi.
Differences between the populations of the same species were found for seed size, circularity and roundness but not in shape quantification by similarity with a geometric model (J index), thus indicating the stability of the shape measured by comparison with a geometric model in comparison with the other measurements. Funding: Project "CLU-2019-05-IRNASA/CSIC Unit of Excellence", funded by the Junta de Castilla y León and co-financed by the European Union (ERDF "Europe drives our growth").

Conflicts of Interest:
The authors declare no conflict of interest. Appendix A Figure A1. Superimposed images of the lateral view of Silene andryalifolia seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A2. Superimposed images of the lateral view of Silene badaroi seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A3. Superimposed images of the lateral view of Silene gazulensis seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A4. Superimposed images of the lateral view of Silene hifacensis seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A5. Superimposed images of the lateral view of Silene tomentosa seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A6. Superimposed images of the lateral view of Silene auricolifolia seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A7. Superimposed images of the lateral view of Silene hicesiae seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A8. Superimposed images of the lateral view of Silene ichnusae seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A9. Superimposed images of the lateral view of Silene mollissima seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A10. Superimposed images of the lateral view of Silene oenotriae seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm. Figure A11. Superimposed images of the lateral view of Silene velutina seeds, the corresponding average silhouette, model LM5 and two seeds of this species. Bar represents 1 mm.