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Migratory responses of ovine neutrophils to inflammatory mediators in vitro and in vivo

The migration of 111 In‐labeled ovine neutrophils toward a range of inflammatory mediators was examined in vitro using a 48‐well chemotaxis chamber. Typical curves were obtained for the chemotactic response to zymosan‐activated plasma (ZAP, a source of C5a) and interleukin‐8 (EL‐8). In contrast, leukotriene B4 (LTB4), platelet‐activating factor (PAF), interleukin‐lα (ILlα), tumor necrosis factor α (TNF‐α), N‐formyl‐methionine‐leucyl‐phenylalanine (fMLP), and endotoxins from Escherichia coli and Pseudomonas aeruginosa failed to induce neutrophil migration in vitro. Of these mediators LTB4, ZAP, lL‐lα, TNF‐α, IFN‐γ, and IL‐8 have been reported to induce neutrophil accumulation in skin of sheep [1], and in the current study E. coU endotoxin was a potent inducer of 111In‐labeled neutrophil accumulation and plasma leakage in skin. In contrast, PAF induced intense plasma leakage but failed to induce accumulation of 111In‐labeled neutrophils in skin. Histologic examination of skin sites receiving PAF confirmed the failure of PAF to stimulate neutrophil extravasation. FMLP lacked inflammatory activity in skin. Coinjection of actinomycin D did not abrogate recruitment of neutrophils to skin sites receiving LTB4; thus neither induction of endothelial adhesion molecules nor synthesis of IL‐8 was necessary for LTB4 to exhibit inflammatory activity in vivo.