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Demonstration of acrosomal membranes using the hypoosmotic swelling test

Summary. The state of the acrosomal membranes in human spermatozoa was studied by means of the hypo‐osmotic swelling test and indirect immunofluorescence using anti‐boar outer acrosomal membrane antibodies. The swelling phenomenon observed in the acrosomal region was characterized by expansion of the plasma membrane without modification of the outer acrosomal membrane.