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Detection of Some Virulence Factors Genes of Staphylococcus Aureus Isolated from Patients with Ventilator-Associated Pneumonia in Al-Hilla\Iraq

A total of (96) lavage specimens were taken from patients with ventilator associated pneumonia (VAP), and bronchioalviolar lavage (BAL), during the period from February to June (2018) admitted to the Al-Hillah General Teaching Hospital, Al- Imam Al-Sadiq Hospital and Tiba Center. Among them 24(25%) of specimens were found to be with S. aureus bacteriuria. In the present study, it was observed that the number of patients with 30(31.2%) isolates were classified as Staphylococcus spp. Among them, 24(25%) isolates were identified as Staphylococcus aureus , the remaining isolates 6(25%) were classified as coagulase negative Staphylococcal species. Molecular detection of virulence ( hla , tst , agrII and pvl ) genes, molecular investigation among all Staphylococcus aureus isolates showed that all isolates (100%), (91.6%), (0.0%), (0.0%) respectively.