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In Vitro Fertilization Studies On In Vitro Matured Cattle Oocytes Using Heparin,Epinephrine And Pencillamine In The Culture Medium

Sire effect on in vitro fertilization on in vitro matured cattle oocytes were tested using frozen thawed semen. Oocytes were collected from the ovaries of slaughtered cattles. Follicular fluid were aspirated and screened for good quality oocytes. It is then washed and cultured in the maturation media. After incubation at 38 o C for 12-15 hours in a CO2 incubator it is fertilized with washed semen in the fertilization medium. It is again incubated at 38 o C for 12-15 hours keeping in a CO2 incubator. Oocytes are then taken out washed, fixed, stained and observed for fertilization. Fertilization rate for different bulls were calculated. Semen from different bulls found to have different rate of fertilization on in vitro matured oocytes. The fertilized embryo can be transferred to embryo culture medium for further development to blastocyst and stem cell production.