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Isolation of the Acetyl-CoA Synthase Gene from the Corn Smut Pathogen, Ustilago maydis

Summary: The acetyl-CoA synthase (acuA) gene from Ustilago maydis has been isolated using a fragment of the acu5 gene from Neurospora crassa as a heterologous probe. The U. maydis acuA gene transformed Acu mutants of U. maydis to acetate utilization. Transformation was accomplished by integration of the vector sequences into the chromosomal DNA.