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Sequence Analysis of Mutant Chick Embryo DNA

Riboflavin Binding Protein is a transport glyco‐phospho‐protein found in all oviparous species, required for transport of the vitamin riboflavin needed for proper embryonic development. A mutant strain of chickens cannot synthesize the protein; the embryos lacking riboflavin die after the thirteenth day of incubation. DNA was extracted from the embryos. Several kits were utilized and compared to determine the best method to isolate pure DNA in large yield. Sequence comparisons to wild‐type white leghorn chickens are used to confirm the DNA sequence causing the mutation.