{'fraction_non_alphanumeric': 0.03723008190618019, 'fraction_numerical': 0.012658227848101266, 'mean_word_length': 4.894736842105263, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Genetic Differentiation Between Mullus barbatus from the Western Part of the Black Sea and Mullus surmuletus (Pisces, Mullidae) from the Mediterranean Sea

Genetic divergence and phylogenetic relationship of two species Mullus barbatus from the western part of Black Sea (Varna) and M. surmuletus from the Mediterranean Sea (Thessaloniki) were investigated using the electrophoretic data from enzymatic systems, codifying for 15 putative loci, and the patterns of general muscle proteins (PROT) coded from nine loci. Several loci PROT-4 ∗ , PROT-5 ∗ and PROT-8 ∗ as well as two mMDH and two sMDH loci, and LDH-A ∗ showed different electrophoretic patterns among species and can be used as species-specific markers. Only one esterase locus ( EST-9 ∗ ) was found to be polymorphic for both species. The remaining enzymes and proteins were monomorphic. In this study for the first time existence of hybrids between two species were reported. Hybrids were registered in the Mediterranean Sea (Thessaloniki) as well in the northeastern part of Black Sea (Balshoj Utrish) using electrophoresis and isoelectric focusing methods. Genetic distance D Nei (0.526) and time of divergence (t Nei = 3 215 000 years) between M. barbatus (Varna Bay) and M. surmuletus (Thessaloniki) give evidence for existence of these two well diverged species in one genus.