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Isozyme analysis of stone flounder in the coastal waters of Shandong

Horizontal starch gel electrophoresis was employed to investigate the tissue-specificities of isozymes and the genetic structure of the wild stone flounder (Kareius bicoloratus Jordan et Synder) population distributed in the coastal waters of the Shandong Peninsula. The analysis was conducted on 43 individuals of the fish collected in Haiyang. The performances of 17 varieties of isozymes in 8 kinds of tissues were screened preliminarily in TC-7.0 buffer system. The results showed that the screened isozymes displayed remarkable tissue-specificities. Besides, 14 varieties of enzymes (AAT, ADH, EST, GPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) from both muscle and liver tissues were selected for the genetic analysis. Fourteen isozymes systems are encoded by 21 loci, and 6 of them were polymorphic, i.e.,GPI-2*, IDHP-2*, MDH-1*, MPI-1*, PGM-3* and SDH*. The proportion of polymorphic loci is 0.285 7 (P 0.99). The mean values of observed and expected heterozygosities (H o H e) are 0.032 1 and 0.028 3 respectively and the average effective number of alleles is 1.067 5. Genetic deviation index shows heterozygotes surplus at these loci. There is complete or approximate complete replacement of alleles at AAT*, ADH*, GPI-1*, MDH-1*, MDH-2*, MDH-3*, MPI-1*, PGM-1*, PGM-2*, SOD*, IDHP*, LDH* and SDH* between the stone flounder and the spotted halibut. Nei’s genetic distance between these two species is 1.311 7.