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IgG receptors on eosinophils--using immune scanning electron microscopy.

Using carboxylate modified latex particles covalently conjugated with anti-IgG, IgG receptors on eosinophils were examined with scanning electron microscopy (SEM). After block and inhibition tests, significant number of latex particles were confirmed to bound on the surface of eosinophils. Earlier reports described that density of eosinophils decreased in hypereosinophilic patients and the heterogeneity of eosinophils came into focus. Our experiment revealed that eosinophils of hypereosinophilic patients had more IgG receptors than those of normal volunteers. This difference might be due to the heterogeneity of eosinophils.