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Formation of lipid-bound N-acetylhexosamine derivatives in yeast particulate fractions.

1. Particulate fraction (105 000 g) from Saccharomyces cerevisiae catalyses the transfer of N-acetylglucosamine from UDP-N-acetyl[14 C] glucosamine into lipid fraction as well as to insoluble polymer. 2. The evidence presented is in favour of the lipid containing the N-acetylglucosamine mono-, di- and tri-saccharide derivatives of dolichyl diphosphate. 3. The presence of a transferase synthesizing dolichyl-linked sugars in mitochondrial fraction is also reported.