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Effect of Short-Term Castration and Starvation upon Hypothalamic Content of Luteinizing Hormone-Releasing Hormone in Adult Male Rats 1

Summary A sensitive and specific radio-immunoassay for hypothalamic LH-RH has been described. Within 7 days after castration there is a significant decline in hypothalamic content of LH-RH in adult male rats. Total starvation for 7 days does not affect hypothalamic content of LH-RH in either intact or castrated rats. The authors thank the Hormone Distribution Officer, NIAMDD, for reagents for the radioimmuno-assay of rat LH and rat FSH. They also thank Mrs. E. Morris for secretarial assistance.