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ANALYSIS OF THE PRODUCTIVITY OF EGGS AND LARVAE BY THE FEMALE NEMATODE MELOIDOGYNE INCOGNITA IN LABORATORY CONDITIONS

Root-knod nematode is an endoparasite of the root system. Causes the disease
meloidogynosis and is an important factor in reducing the yield of most crops. The
laboratory is working to maintain a population of the nematode Meloidogynе incognita
in culture for use in experiments on the development of plant protection products.
During laboratory work with meloidogyna, it was noticed that this species reproduces
very hard in winter. For the analysis, data on the collection of eggs and larvae from
2015 to 2021 were summarized. The work includes the results of experiments on
the "pure control" option. Data obtained only from cucumber plants with an initial
invasive background of 100 larvae per 100 g of soil were compared. Egg sacs were
collected under a binocular microscope using two preparatory needles, separated
from the root-knot and transferred to a second Petri dish with water. Hatched larvae
begin to appear after 3 days for two weeks. The larvae were collected from the bottom
of a Petri dish using a pipette. Live larvae were counted. The remaining ootheca was
crushed on a slide and the eggs remaining in them were counted. It was found that in
winter the ootheca contains 5 times fewer eggs compared to summer.