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Accumulation of enamel constituents in Streptococcus mutans plaque during intraoral demineralization.

Studies were carried out with an intraoral demineralizing system in order to determine whether calcium and inorganic phosphate (Pi) accumulate in plaque during active demineralization of enamel. Blocks of bovine enamel were coated with Streptococcus mutans and were carried in palatal appliances worn by human volunteers. Demineralization was determined as changes in the iodide penetrability (delta Ip) of the enamel surfaces. Ca and Pi were determined in the extracellular spaces of the synthetic plaque. Delta Ip increased with time after administration of rinses containing 5% (w/v) sucrose, while plaque pH dropped and then returned toward neutrality. Ca increased to 10.9 +/- 2.8 mmol/l at 30 min, while Ca2+ and Pi rose to 3.0 +/- 2.1 and 9.5 +/- 3.1 mmol/l, respectively. The Ca:Pi ratio was 1.15. Rinses with 10% (w/v) sucrose gave similar results. Concentrations of Ca and Pi were considerably higher than those in saliva. Accumulation of the mineral constituents was shown to be dependent on metabolic activity of the S. mutans plaque, and experiments in which enamel blocks were replaced with blocks made of acrylic plastic gave Ca and Pi concentrations of 2.5 +/- 0.6 and 6.6 +/- 2.4 mmol/l, respectively, demonstrating that most of the Ca and about one-third of the Pi were derived from enamel. The data suggested, furthermore, that Ca and Pi were partially bound to complex macromolecules, and that part eventually recrystallized as mineral within the plaque.