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Evidence for a Fourth Hydrogenase in Desulfovibrio fructosovorans

ABSTRACT A strain devoid of the three hydrogenases characterized for Desulfovibrio fructosovorans was constructed using marker exchange mutagenesis. As expected, the H2-dependent methyl viologen reduction activity of the strain was null, but physiological studies showed no striking differences between the mutated and wild-type strains. The H+-D2 exchange activity measured in the mutated strain indicates the presence of a fourth hydrogenase in D. fructosovorans.