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Composite activities on B cells of products released by T cells activated by concanavalin A

Supernatants from murine spleen cells cultured for 48 h in the presence of 1.0 μg of concanavalin A (Con A) induced polyclonal antibody synthesis in cultures of spleen cells from both normal and athymic mice in the absence of exogeneous antigen. Moreover, the Con A‐induced supernatant rescued B cells which had been rendered unresponsive by a tolerogen [haptenated poly(DGlu,DLys)]. The capacity of the supernatant to induce cell proliferation was studied under both high and low‐density culture conditions. In contrast to antibody secretion, proliferation was only detectable in low‐density cultures.