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Lipase-catalysis of α-linolenic acid-rich medium- and long-chain triacylglycerols from perilla oil and MCTs with reduced by-product.

BACKGROUND
Medium- and long- chain triacylglycerol (MLCT) is a functional structural lipid that can provide the human body with essential fatty acids and faster energy supply. This study aimed to prepare MLCTs rich in α-linolenic via an enzymatic interesterification of perilla oil and MCTs, catalyzed by Lipozyme RM IM, Lipozyme TL IM, Lipozyme 435 and Novozyme 435 respectively.


RESULTS
The effects of lipase loading, concentration of MCTs, reaction temperature and reaction time on the yield of MLCTs were investigated. It was found that the reaction achieved more than 70% yield of MLCTs in triacylglycerols under the conditions of 400 g kg-1 MCTs and 60 g kg-1 lipase loading after equilibrium. Also, a novel two-stages deodorization was applied to purify the interesterification products. The triacylglycerols reach over 97% purity in the products with significant removal (p< 0.05) of the free fatty acids, and the trans fatty acids was strictly controlled below 1%. Further, there was more than 40% of α-linolenic in the purified products with long-chain fatty acids occupying mostly the desired sn-2 position in acylglycerols, which are more active in hydrolysis.


CONCLUSION
A series of novel α-linolenic acid-rich medium- and long-chain triacylglycerols were prepared. Under appropriate reaction conditions, the yield of MLCTs in triacylglycerols was above 70%. A novel two-stages deodorization can be used to promote the elimination of FFA and limite the generation of trans fatty acids. This article is protected by copyright. All rights reserved.