{'fraction_non_alphanumeric': 0.03179041555536436, 'fraction_numerical': 0.012604319022627548, 'mean_word_length': 5.2846715328467155, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Fruit Pod Extracts as a Source of Nutraceuticals and Pharmaceuticals

Fruit pods contain various beneficial compounds that have biological activities and can be used as a source of pharmaceutical and nutraceutical products. Although pods or pericarps are usually discarded when consuming the edible parts of fruits, they contain some compounds that exhibit biological activities after extraction. Most fruit pods included in this review contain polyphenolic components that can promote antioxidant effects on human health. Additionally, anti-inflammatory, antibacterial, antifungal and chemopreventive effects are associated with these fruit pod extracts. Besides polyphenolics, other compounds such as xanthones, carotenoids and saponins also exhibit health effects and can be potential sources of nutraceutical and pharmaceutical components. In this review, information on fruit pods or pericarp of Garcinia mangostana, Ceratonia siliqua, Moringa oleifera, Acacia nilotica, Sapindus rarak and Prosopis cineraria is presented and discussed with regard to their biological activity of the major compounds existing in them. The fruit pods of other ethno- botanical plants have also been reviewed. It can be concluded that although fruit pods are considered as being of no practical use and are often being thrown away, they nevertheless contain compounds that might be useful sources of nutraceutical and other pharmaceutical components.

either red maroon or green. As another example, ripe mangosteens turn dark purple from green. Some pods turn brown, black or dark brown. The color results from pigments and phytochemicals such as chlorophyll, carotenoids and phenolics [12]. Red fruits are associated with compounds like lycopene, ellagic acid, quercetin and hesperidin, while orange and yellow fruits are usually linked to β-carotene, zeaxanthin, flavonoids and vitamin C [13]. Chlorophyll, lutein, zeaxanthin and β-carotene are related to green fruits [14]. Blue and purple colors of fruits are often applied to resveratrol, quercetin and ellagic acid [15]. Finally, white colour is associated with the presence of β-glucans and lignans [16]. Although tristimulus colorimeters can be used to measure the visual appearance of fruit pericarp [17], identifying the profiles of the pericarp content should be carried out by analytical instrumentation to confirm the exact active compounds that exist in it.
This review focuses on bioactive compounds or phytochemicals of fruit pods with beneficial health effects that have potential pharmaceutical and nutraceutical applications. This review provides researchers with useful knowledge and guidance in future experimental work on developing pharmaceutical and nutraceutical products from this part of fruits. The available literature was searched using Google Scholar, Science Direct and Springer Link for scientific publications published during the period 1992-May 2012 describing beneficial aspects of bioactive components from fruit pods. In fact, certain fruits like mangosteen, carob, Acacia nilotica and Moringa oleifera and their pods have been extensively studied. Keywords used for the search were bioactive compounds, fruit pods, nutraceutical, pharmaceutical and plant names. This review focuses on the beneficial effects of bioactive compounds from fruit pods through the review by available in-vitro, pre-clinical and/or human trial studies in the related literature.

Garcinia mangostana
G. mangostana is also known as mangosteen or manggis in Malaysia. Although this plant grows well in tropical areas of the World, including Malaysia, Thailand and Indonesia, the nutraceutical products of mangosteen have been patented by Garrity et al. [18] from the USA and successfully marketed worldwide. Two major groups of phytochemicals in the mangosteen pericarp are the xanthones and phenolics. The antioxidant properties of G.mangostana is related to the presence of high levels of phenolic compounds (tannins) in its methanol, and ethanol extracts [19]. The phenolic compounds in mangosteen, including afzelechin, epiafzelechin, catechin, epicatechin, gallocatechin and epigallocatechin, can proiduce oxygen radical scavenging capacities as high as 1.7 × 10 4 μmol TE/g, which is greater than those of grape seed and pine bark [20]. The quantity of phenolic compounds and antioxidant activity in the mangosteen pericarp is ten times and 20 times greater, respectively, than in the white edible parts.
Medicinal properties of G. mangostana have been summarized by Chaverri et al. [26] and show that the extract of this pericarp has antioxidant, antitumor, anti-inflammatory, antiallergy and antimalarial, besides anti-bacterial/viral properties. In addition, studies on the effect of mangosteen extract on obesity genes have also shown positive results [27][28][29]. The extract also reduced cholesterol level in rats [30]. The crude methanolic extract of mangosteen strongly inhibited human breast cancer cell profileration at ED 50 of 9.25 μg/mL [31] and colon cancer cells [32]. In another study, crude methanolic extract of mangosteen also inhibited quinone reductase activity [33]. Nakatani et al. [34] studied the anti-allergy and anti-inflammatory effects of mangosteen pericarp extract in comparison with that of a traditional plant extract of Rubus suavissimus in Japan using in vitro studies. Mangosteen ethanol extract (40%) inhibited histamine release and prostaglandin synthesis with greater effect. The extract also inhibited the synthesis of cyclooxygenase [35], a rate-limiting enzyme, functionally important in fluid and electrolyte homeostasis, gastric acid secretion and platelet aggregation. Ethanolic (50%) extract of G.mangostana has been shown to exhibit neuroprotective activity in vitro [36]. Human subjects who received mangosteen dietary supplements for one month also showed significantly enhanced immune responses [37]. On the other hand, polysaccharide from mangosteen pericarp extracted using hot water and precipitated with ethanol stimulated phagocytic cells and demolished intracellular bacteria, namely Salmonella enteritidis. The extract contained D-gallacturonic acid, L-rhamnose and D-galactose [38]. A different species of mangosteen, Garcinia cochinchinensis, also exhibited strong anticancer activity following the presence of the compound guttiferone. The extract of G.cochinchinensis pericarp was higher in xanthone as well as trimethyl citrate [39].
Most of pharmaceutical and nutraceutical properties of G. mangostana pod extract in the scientific papers were studied using in-vitro methods where the effects varied between cells and species depending on host metabolism and bioavailability of the extracted compounds. For example, the effective concentration of extracts to cancer cells of human was ten times higher than needed for mouse to inhibit proliferation of cells in-vitro. Therefore, human clinical trials are needed due to the different in-vitro potency. Meanwhile, the extraction method such as type and percentage of solvent used can affect the beneficial response such as a hot water prepared extract is lacking in glucose, as previously shown [38], thus requiring detailed identification of the compounds present the extract.

Ceratonia siliqua
C. siliqua is a plant of the Mediteranean regions. Also known as carob, the crude extract of the plant pod exhibited antioxidant properties higher than certain known polyphenols such as catechin, quercetin and gallic acid alone [40,41], due to the presence of carotenoids such as lutein, lycopene, α-carotene and β-carotene. Compound identification using High Performance Liquid Chromatography (HPLC) showed that carob pods contain flavonoids of quercetin glycosides, catechin and epicatechin gallate, polyphenols of gallic acid and ellagic acid, and anthocyanins such as proanthocyanidins and ellagitannins [42], as well as epigallocatechin gallate [43].
Avallone et al. [44] revealed that extract of carob pod can be used as a natural product with anxiolytic-sedative effects and act as a chemopreventive agent. The presence of gallic acid, epigallocatechin-3-gallate and epicatechin-3-gallate in carob pod can exert antiproliferative effects in-vitro [45]. Proliferative effects are related to cancerous cells that grow and increase rapidly. The patented aqueous extract of C. siliqua pod revealed the antioxidant activity of the extract along with potential antitumor activity [43]. The extract has equivalent antioxidant level to tea, but without the stimulant effects of caffeine and theophyline. The extract was prepared using hot distilled water for 15 minutes, then filtered and evaporated to dryness. The polyphenols content of the extract was 1.36 mg/g of pod powder. The pod extract at a concentration of 80 μg/mL was shown to effectively inhibit proliferation in liver tumor cells after 48 hours of treatment in-vitro. The extract of carob pod also has an antidepressant effects as exhibited in a study by Agrawal et al. on mice using tail suspension and forced swim tests [46]. The anti-diarrhea effect of carob pod dietary fiber, as patented by Mark et al. was observed in tube-fed patients [47]. Dietary fiber of carob pod also proved to reduce total cholesterol and LDL-c levels significantly after six weeks of consumption before breakfast in 49 volunteers with mild to moderate cholesterol levels [48].
Generally, pod extracts of C. siliqua were shown to posesses health benefits for humans. However, the in vitro antiproliferative and other beneficial effects need to be confirmed and extensively studied to evaluate its effectiveness in-vivo. The high antioxidant level of C. siliqua pod extract suggesting its potential development as a nutraceutical or pharmaceutical product.

Moringa oleifera
M. oleifera is a semi-arid plant [49], which can be found in tropical and subtropical climates. The plant, which is also known as the horseradish tree, can be found in India, Thailand, Africa and Indonesia. Research has shown that biologically active components present in this plant contributing to its health benefits are simple sugars and compounds called glucosinolates and isothiocyanates, including 4-(4′-O-acetyl-α-L-rhamnopyranosyloxy)benzyl isothiocyanate, niazimicin, pterygospermin, benzyl isothiocyanate and 4-(α-L-rhamnopyranosyloxy)benzyl glucosinolate, as well as carotenoids [50]. Two other compounds, niaziridin and niazirin, were also detected in M. oleifera pod by reverse phase HPLC [51].
Reportedly, M. oleifera pod has antioxidant activity [52], which is due to the presence of carotenoid compounds. The pod contains high amounts of bio-enhancers in comparison with the bark. It can be used to reduce cholesterol and glucose in blood with the safe intake level determined at ≤1,000 mg/kg body weight [53]. The ethanolic pod extract also showed hypotensive activity at a 30 mg/kg dose [54]. According to Jakansul et al. [55], the hypertensive effect was due to the thiocarbamate glycoside extracted from the pod. Intake of pod powder of M. oleifera was also shown to cause significant reduction in total cholesterol and total lipid levels of high lipid and high cholesterol diet-induced rabbits [56].
Antiurolithiatic activity was also exhibited by the extract of Moringa oleifera pods as studied by Vijayalakshmi et al. [57]. The extraction was carried out using boiling water for six hours and evaporation to dryness. Urolithiasis-induced albino rats were treated with the extract for one month. In comparison with the control group, rats fed with the plant extract at 400 mg/kg body weight showed significant reduction in stone weight.
A significant anti-inflammatory action was revealed by Rakesh et al. [58] in a study of the effects of M. oleifera ethanolic extract on carrageenan-induced paw edema in albino mice. The study was carried out to compare the relative effect of pod extract to diclofenac sodium, where the required dose of pod extract was 1,000 mg/kg. Methanolic extract of fresh M. oleifera fruits also exhibited an anti-inflammatory effect in-vitro as observed by Cheenpracha et al. and Muangnoi et al. [59,60].
Aqueous ethanolic extract of M. oleifera also has a hepatoprotective effect, which was exhibited in albino mice with induced hepatocarcinogeniticity [61]. The effectiveness of pod extract was also apparent in repairing liver damaged by carbon tetrachloride in albino mice at 750 mg/kg [62]. Moreover, a niaziridin-rich extract fraction of M. oleifera pods enhanced the bioactivity of several antibiotics (rifampicin, tetra cycline and ampicillin) against bacteria and facilitated drug absorption through the gastrointestinal membrane [63].
The above studies indicate the beneficial effects on health by M. oleifera pod extracts. Although most of the effects were observed in animal models, these benefits may be extended to humans in the form of dietary supplements in the future.
Formerly, the plant extract was used as a tanning [64] and dying agent. The plant itself was very useul since it provided water and insect-resistant wood, fodder for livestock and folk medicines. The pods were used to treat fever, diarrhea, diabetes, sore gums and skin diseases. Studies indicate that the pod extract of A. nilotica exhibits antioxidant, chemopreventive, antidiabetic, hypolipidemic and antiplasmodial activities [64,65]. The extraction was carried out by soaking of 44.2 g A. nilotica pod overnight in 500 mL of methanol three times. After filtration and solvent removal, the crude extract was fractionated using the solvents n-hexane, methanol, ethyl acetate, buthanol and water [8]. The aqueous methanolic extract of Acacia nilotica pod also caused significant reduction of blood glucose, plasma total cholestrol, triglycerides and low-density lipid (LDL) levels in alloxan-induced diabetic rabbits at 400 mg/kg body weight in a one-month treatment [66]. The ethyl acetate fraction of the pod exhibited antidiarrheal activity in albino rats at 400 mg/kg body weight [67]. Freeze dried extract of A. nilotica pods showed bactericidal activity against an extended spectrum of β-lactamase (ESBL) and methicillin-resistant Staphylococcus aureus (MRSA), as studied previously [68]. Another species of acacia, Acacia auiculiformis, has abundant amounts of acaciaside (tripernoid) in its pericarp that exhibits antifilarial activity [69].
Nutraceutical products could possibly be produced by using A. nilotica pod extract as a source of dietary fibre. Even though effective antidiarrheal effects have been determined in animals models, its efficacy in humans also need to be addressed in future studies.

Sapindus rarak
Sapindus rarak is the soapberry plant, known in Indonesia as lerai or kelerak. Traditionally, the pericarp was used for skin disorders. Four triterpene saponins have been identified in S. rarak pericarp, including rarasaponins (I, II, and III), raraoside, along with 13 other known saponins and four known acyclic sesquiterpene glycosides. A study revealed that the methanolic extract of Sapindus rarak pericarp contains saponins that inhibited pancreatic lipase and reduced lipid digestion in-vitro with an IC 50 of 614 µg/mL [70]. The antiobesity effects of this plant were more strongly exhibited by raraoside and rarasaponin in comparison with the saponin compounds. A study on the effects of the methanolic extract of Sapindus rarak on plasma trigylceride elevation was conducted in mice treated with olive oil [71]. The results indicated that elevation of triglycerides was inhibited at a dose of 200 mg/kg by the saponin constituents, including hederagenin. The extract also exhibited inhibitory effects on tumor necrosis at a concentration of 30-100 µM, as reported by Morikawa et al. [72].
S. rarak has potential to be used in the formulation of nutraceutical products with weight loss effects due to the presence of bioactive compounds that inhibit lipid digestion. However, the effective dose of extract must be determined prior to its incorporation into products due to its high content of saponins.

Prosopis cineraria
P. cineraria or Khejri is also known as the king of the desert. The plant grows well in Western and Southern Asia, including Afghanistan, Iran, India, Oman, Pakistan and Saudi Arabia. When the boiling water extract of its pods is fractionated using methanol and trichloromethane, it results in the isolation of compounds such as 3-benzyl-2-hydroxy-urs-12-en-28-oic acid and maslinic acid-3-glucoside (triterpernoids); linoleic acid (fatty acid); prosophylline (piperidine alkaloid); 5,5′-oxybis-1,2benzanediol; 3,4,5-trihydroxycinnamic acid 2-hydroxyethyl ester; and 5,3′,4′-trihydroxyflavanone 7-glycoside (polyphenols). In vitro bioassays carried out by Liu et al. [73] using these compounds and crude extract have shown positive results supporting its health benefits in preventing a wide range of illnesses including protein and mineral deficiencies. High antioxidant activity has also been exhibited by the methanolic extract of its pods, which also have indicated antimicrobial activity against Candida albicans [74]. There is also empirical proof that P. cineraria can exhibit estrogenic activity in vitro [75].
Although most of the biological activities showed in studies were proven in animals, there are potential benefits of P. cineraria pod extract as an antioxidant. However, the toxicity effects of the extracts need to be determined as the extract contains piperidine alkaloids which might exert certain adverse effects when consumed, as mentioned by Bahorun et al. [6].

Other Ethnobotanicals
There are several other plant pods which are used in traditional medicines for health and medicinal (ethnobotanical) purposes. However, most of the related publications focus on the biological activity of the pod crude extracts without any detailed identification of the extract itself. Ethanol extract of unripe Bauhinia purpurea exhibited an anti-obesity effect in cholesterol and high fat diet (CHFD)-induced hyperlipidemic rats [76]. As it was observed in this study, feeding the rats with CHFD together with unripe pods at 300 mg/kg/day lowered the body weight increase by 7.4% in comparison with hyperlipidemic rats (13.11%) in the control group. The crude extract of unripe B. purpurea pods was reported to contain carbohydrates, proteins, alkaloids, flavonoids, triterpenes, glycosides and steroids.
Parkia speciosa or stinky bean (petai in Malaysia) has also exhibited antioxidant properties due to its high total phenolic and high flavonoid contents [77]. Oral administration of chloroform extracts of P. speciosa pod to diabetic-induced rats resulted in a significant reduction of blood glucose levels which suggests the potential use of the extract as an oral hypoglyceamic agent [78]. The methanolic extract of the Parkia speciosa pod also exhibited a significant antiangiogenic effect [79]. The ethanol extract of Cassia occidentalis pod showed antifungal activity towards Aspergillus clavatus at 100 μg/mL, which was equal to the concentration required using commercial drugs such as nystatin and griseofulvin. Although a higher minimum inhibitory concentration (MIC) value was needed to inhibit Candida albicans and Aspergillus niger (125 and 500 μg/mL, respectively), the inhibitory effect of the pod extract was comparable to the said drugs [80].
The extract of Albizia julibrissin Durazz pod exhibits antioxidant activity and a strong inhibition against E. coli, S. aureus, B. megaterium, B. subtilis and S. typhi [81]. However, a report [82] indicated that methanolic extract of Albizza lebbeck pod decreased the fertility of male albino rats significantly, without stating whether the effect was reversible or not. Nearly, 45% of the pod compounds are aromatic. n-Hexane extract of Samanea saman pods contains phytochemicals such as cyanidin, catechin, epicachin, anthocyanin monoglycones, delphinidin and malidin. Potent antibacterial properties, including against Candida albicans, have been exhibited by the ethyl acetate fraction of Samanea saman at 10,000 ppm [83]. Moderate antibacterial activity against Bacillus subtilis and Pseudominas aeruginosa was exhibited by the water extract of Cassia fistula pod [84].
Fruit pericarp of Emblica officinalis (used in Ayurveda treatment) contains hydrolysable tannins such as emblicanin, punigluconin and pedunculagin with significant antioxidant properties [85]. Extracts of Catalpa bignonoides pods exhibit anti-inflammatory and antinociceptive effects due to the presence of saponin, sterol or phenol compounds in the pods [86]. These properties are also exhibited by Tecoma sambucifolia [87]. Anti-inflammatory activity has also been observed in polysacharide extract of Caesalpinia ferrea pods [88]. Beneficial effects of Astragalus hamosus pod extract on edema-induced rats has also been reported by Hakim et al. [89], who observed a significant reduction in the size of rats' hind paws 3 hours after injection. The aqueous and alcoholic extracts of the pod exhibit a similar significant effect. Methanol extract of Caesalpinia pulcherrima pods showed significant anti-inflammatory effects when edema-induced rats were fed with pod extract at 400 mg/kg body weight in comparison with the control rats [90]. Pod extract of Cassia italica also exhibited anti-inflammatory effects on carragenan-induced paw swelling. The aqueous ethanol (80%) extract also exhibited anti-pyretic activity [91].
The summary of biological activity of compounds of fruit pods associated with health benefits is presented in Tables 1 and 2. Some potential products that can be suggested from the pod extracts of ethnobotanical plants are those such as anti-inflammatory drugs, antibacterial creams and even dietary supplements which may assist in reducing blood pressure and blood glucose concentration. Although, human clinical trials are a must, positive findings from animal and in vitro studies indicate that the benefits of pod extracts should be explored further.

Conclusions
This review has highlighted that most extracts from plant pods (including mangosteen and M. oleifera) contain compounds with antioxidant, anti-inflammatory, antibacterial, antifungal and other biological activities. The antioxidative properties are mostly related to their high content of polyphenolics such as catechin and gallic acid. In addition, the presence of carotenoids in some pod extracts, such as in carob, can increase the antioxidant capacity, which may suggest a synergystic effect between polyphenols and carotenoid compounds in the pod extract, or with xanthones as in G. mangostana pod extract. Potent antibacterial activity against several microbes including E. coli and S. aureus, as well as antifungal activity towards C. albicans, were exhibited by the pod extracts. Anti-obesity effects have also been attributed to the extracts from fruit pods of Sapindus rarak, Garcinia mangostana, Moringa oleifera and Acacia nilotica. In addition, certain pod extracts can also offer anti-diarrheal effects due to their high dietary fibre content. With these known biological activities, therefore, plant pod extracts can be promoted for usage in pharmaceutical and nutraceutical products in the near future.