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Acid Resistance of Enamel Subsurface Lesions Treated with Casein Phosphopeptide Amorphous Calcium Phosphate Fluoride.

PURPOSE
The purpose of this study was to evaluate the acid resistance of enamel subsurface lesions treated with casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF).


METHODS
Fifty extracted primary molars with a standardized window on enamel were immersed in a demineralizing solution for 72 hours to produce subsurface enamel lesions. They were sectioned in a buccolingual direction; one half of the sample was treated with the remineralizing agent CPP-ACPF and the other half remained untreated (control group). After 10 days, the sample was evaluated quantitatively using energy dispersive X-ray spectroscopy. The treated samples were reimmersed into the demineralizing solution for 72 hours and the mineral content was re-evaluated.


RESULTS
The mean calcium content of the remineralized samples was significantly higher than that of the control group (P<.001) and was higher than the second demineralization values. The mean calcium content of the second demineralization was significantly higher than those of the control group (P<.001).


CONCLUSION
CPP-ACPF increased the resistance of enamel surfaces to further demineralization.