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Characterization of the Dual Pathways for Bacillus halodurans Asparaginyl‐tRNA Formation

Protein synthesis requires the attachment of an amino acid to its cognate transfer RNA (tRNA). Two distinct pathways for attaching asparagine (Asn) to tRNAAsn are known: the direct pathway, in which Asn is directly attached to tRNAAsn by the enzyme AsnRS; and the indirect pathway, in which Asn is attached to tRNA by a non‐discriminating AspRS and GatCAB. Bacillus halodurans uses both routes for Asn‐tRNAAsn formation. To better understand why B. halodurans possesses both pathways, we are purifying its AsnRS and AspRS in order to test and compare their activities under different chemical conditions such as reactive oxygen species and pH. This research will provide insight into how B. halodurans has adapted to survive in different environmental conditions.