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Measurement of the affinity of anti‐D in the serum of immunized mothers and in immunoglobulin preparations with unlabeled antibodies

BACKGROUND: Few data are available on the affinity of maternal anti‐D responsible for hemolytic disease of the fetus and the newborn (HDN) and of anti‐D used for the prophylaxis of that disease. A method was recently described to measure the affinity (Ka) of untagged anti‐D monoclonal antibodies (MoAbs). In this work, the same method was applied to determine the Ka of polyclonal anti‐D.