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Extended storage of granulocyte concentrates mobilized by G‐CSF with/without dexamethasone and collected by bag separation method

summary The aim of this study was to examine the extended storage of granulocyte concentrates mobilized by granulocyte‐colony‐stimulating factor (G‐CSF) with/without dexamethasone (DEX) and collected by a bag separation method. Ten healthy adult volunteers donated blood three times: twice after granulocyte mobilization by (1) injecting G‐CSF at 3 μg kg−1 subcutaneously (s.c.) and (2) injecting G‐CSF at 3 μg kg−1 s.c. + DEX at 8 mg per oral and once (3) for a baseline control without any forms of mobilization. Granulocytes were collected by a bag separation method. The functions (phagocytosis and oxidative killing levels), viability and levels of interleukin (IL)‐1β, IL‐8, IL‐6 and tumour necrosis factor‐α of granulocytes were measured. The average numbers of granulocytes collected from 200‐mL samples of whole blood from the G‐CSF and G‐CSF + DEX groups were 35·1 × 108 and 49·4 × 108, respectively. Phagocytosis level, oxidative killing level and the viability of the granulocytes mobilized by G‐CSF with/without DEX were well maintained for up to 72 h of storage after collection. The levels of the cytokines increased in a time‐dependent manner. The in vitro phagocytosis level, oxidative killing level and the viability of granulocytes mobilized by G‐CSF with/without DEX and collected by bag separation method can be maintained for as long as 72 h after collection.