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Histochemical Study of the RNA-Effect on the Uteri of Castrated Mice

Summary RNA was extracted from the endometrium of the mature sow by the cold phenol procedure. Injection of this RNA into the lumen of the regressed uteri of castrated mice resulted in a significant increase in alkaline phosphatase activity, primarily in the epithelial layer but not in the endometrium stroma. In addition, structural restorations, such as the increase of epithelial height, proliferation of uterine glands, and enlargement of muscle bundles were observed. However, physiological saline, liver RNA, and endometrial RNA degraded by RNAse or boiling had no appreciable effect. The authors thank Prof. M. C. Niu, Temple University, Philadelphia, Pennsylvania for his sustained interest in this work.