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A smart advanced chemiluminescence-sensing platform for determination and imaging of the tissue distribution of natural antioxidants.

Antioxidants have gained marked attention owing to their ability to prevent the oxidation of biological components and to protect the body from ROS, thereby maintaining human health. Thus, antioxidants rich dietary supplements and natural foods can be effective against oxidative stress and even can act as chemopreventive agents. Therefore, a simple and rapid assay for evaluation of antioxidant capacity and assessment of their distribution profile in natural sources is very vital. Herein we report a rapid, innovative chemiluminescence (CL) platform for the evaluation and visualization of the antioxidant capacity. We found that intense and long-lasting CL was formed upon the redox reaction of quinones, e.g., menadione, with antioxidants, e.g., L-ascorbic acid, in the presence of luminol. The produced CL intensities were proportional to the antioxidants' concentrations with a detection limit of 0.18 μM for the model antioxidant, L-ascorbic acid. As the formed CL was long-lasting, it could be easily captured and detected with a CCD camera. To evaluate the quantification ability of CCD camera, we developed a smart and fast microplate-based assay based on photographing the generated CL with a cooled CCD camera. The photographed CL intensities were linearly proportional with the antioxidant concentrations and then the method was applied for photographing multiple food samples extracts. Ultimately, we utilized our method for the distribution profiling of antioxidant capacity in food cut sections. Samples were dipped in luminol then in quinone, followed by CCD camera photography, without the need for any pulverization/extraction procedure, giving precise antioxidant distribution information.