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Reactive oxygen species (ROS) Play a Key Role in Bile Acid (BA)‐Induced Barrier Dysfunction And Alters the Leak Function of Tight Junctions (TJ) in Human Colon Carcinoma T84 Cells

Dysfunction of the pore and leak pathways of TJ plays a vital role in the pathogenesis of inflammatory and diarrheal diseases. Excess colonic BAs are known to alter mucosal permeability. We have shown that chenodeoxycholic acid (CDCA; 500μM) and its derivative, lithocholic acid (LCA; 50μM) have opposing effects on the barrier integrity of T84 cells (FASEB J 2016 30:1223.29). While CDCA‐induced changes in pore and leak function of TJs are further enhanced by pro‐inflammatory cytokines (PiC [ng/ml]: TNFα[10]+IL‐1β[10]+IFNγ[30]), LCA reversed CDCA± PiC's effects only on TJ leak function. As ROS is known to increase paracellular permeability, we examined its role in BA regulation of TJ dysfunction.