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Pombe: A gene‐finding and exon‐intron structure prediction system for fission yeast

A special program developed by the authors, called Pombe, identifies protein coding regions in the Schizosaccharomyces pombe genome. Linear discriminant analysis was applied to predict 5′‐terminal, internal, 3′‐terminal exons (coding‐exon) and introns. The accuracy of the prediction was tested by cross verifications. The sensitivity, specificity and correlation coefficient for the internal exon prediction were 98·5%, 99·9% and 98·3% respectively at the nucleotide level. Open reading frames were studied and used to predict intron‐less genes: 99·0% of such genes were identified with correct stopping sites. The gene structure was determined by dynamic programming and the prediction achieved 97·0% correlation coefficient at the nucleotide level. The program is available at http://clio.cshl.org/genefinder. © 1998 John Wiley & Sons, Ltd.