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Pru p 3-glycodendropeptides based on mannoses promote changes in the immunological properties of dendritic and T-cells from LTP-allergic patients.

SCOPE
Glycodendropeptides (GDPs) functionalized with mannose can enhance allergen interaction with dendritic cells (DCs) via C-type lectin receptors (CLRs), modulating the immune response. They can present multiple peptides and have potential applications for diagnosis and treatment of food allergy (FA). We analysed the immune response induced by GDPs with mannose and Pru p 3 peptides (mono/tetravalent) with ester (D1 ManPrup3/D4 ManPrup3) or ether linkers (D1 Man-O- Prup3/D4 Man-O- Prup3) in LTP-allergic patients and tolerant controls.


METHODS AND RESULTS
The immunological response induced by GDPs was studied by assessing monocyte-derived-DC maturation, lymphocyte proliferation, cytokine production and basophil response by flow cytometry. Dn ManPrup3 was recognised by DCs via CLRs inducing DC maturation in all subjects. However, CCR7 expression was significantly upregulated in allergic patients compared to tolerant controls. These changes correlated with lymphocyte proliferation and specific production of Th2/Th1 cytokines in allergic patients. Moreover, D1 ManPrup3 did not induce basophil activation.


CONCLUSION
Dn ManPrup3 induces changes in DC maturation and lymphocyte proliferation, indicating specific recognition via CLRs. Prup3-GDPs are recognised by immune cells, inducing a specific immune response and modulating the immunological response in FA patients. The specific geometry of D1 ManPrup3 in particular makes it a potential candidate for specific immunotherapy development. This article is protected by copyright. All rights reserved.