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Experimental study on bacteria disinfection using a pulsed cold plasma jet with helium/oxygen mixed gas

Cold atmospheric plasma jet (CAPJ) is widely used in many fields due to the reactive oxygen species (ROS) and low temperature for heat sensitive products. This paper presents inactivation of bacteria via a pulsed plasma jet with He/O2 mixed gas. To evaluate the disinfection performance, Staphylococcus aureus was used as indicator bacteria for experiments. When the plasma jet dealt with agar plates spraying bacteria, it was found that mixed gas has a better performance than pure inert gas, indicated by disinfection area. The increment of oxygen gas addition had benefit to disinfection ability of plasma jet, while the gas had an opposite effect on jet production in length. The experiments showed the efficacy of Staphylococcus aureus disinfection could reach up to 99.47% via helium/oxygen (2%) plasma jet.