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A comparison of the effectiveness of four biostains in enhancing visualization of the vitreous.

PURPOSE
To compare the effectiveness of four biostains in vitreous staining as a means to facilitate clean anterior vitrectomy.


MATERIALS AND METHODS
Four biostains (fluorescein, indocyanine green, trypan blue, and triamcinolone acetonide) were used to stain the transparent vitreous of human cadaver and monkey cadaver eyes. Results were compared and recorded by digital photography and videography.


RESULTS
Of the four biostains, triamcinolone acetonide best highlighted the vitreous. Although fluorescein, indocyanine green, and trypan blue also produced acceptable vitreous staining, the surrounding ocular structures were slightly stained as well.


CONCLUSION
All four biostains enhanced visualization of the vitreous. However, triamcinolone acetonide best highlighted the vitreous.