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EVALUATION OF ATRAZINE BINDING TO SURFACE SOILS

Atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine), one of the most widely used agrochemicals in the Midwestern United States, has appeared in surface waters at levels above the maximum contaminant level of 3 µg/L. Although atrazine is relatively mobile in soil-water systems, it can associate with a variety of soil components. Our study focused on atrazine interactions with two surface soils from the Hillsdale Reservoir Basin in eastern Kansas. One soil was collected from an agricultural field (3.4% SOM), while the other was obtained from an adjacent forested area (6.2% SOM). U-ring- 14 C-labeled atrazine was used in sterile bottlepoint adsorption experiments with synthetic “surface water” to create adsorption isotherms. The distribution of “bound” atrazine was then determined by “surface water” extractions (quickly desorbed), ethyl acetate extractions (slowly desorbed), and alkali extractions (humic and fulvic acid bound). Atrazine remaining on each soil component after extraction was determined by combusting the soil at 925°C and counting the 14 CO 2 on a liquid scintillation counter. At least 70% of the initially sorbed atrazine was quickly desorbed, and soil type did not have a significant effect on atrazine distribution. The presence of the soil enzyme, horseradish peroxidase, was determined to have little effect on the nature and extent of atrazine binding.