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The genetic diversity of three populations of Potamocorbula amurensis based on mitochondrial COI and 16S rRNA gene

Mitochondrial COI and 16 S r RNA gene fragments of three wild populations Potamocorbula amurensis were amplified with universal primers; the PCR products were sequenced, and 658 bp and 450 bp nucleotide sequences were obtained. The T, C, A, G and A+T contents in the fragment(COI/16 S r RNA) were 45.4%/32.0%, 13.5%/13.3%, 20.7%/29.3%, 20.4%/25.3%, and 66.1%/61.3%, respectively. The AT content was higher than the GC content. The phenomenon was similar to the results of other mollusca. 24 haplotypes and 43 polymorphisms were defined among COI from three populations. Nine(9) haplotypes and 19 polymorphisms were defined among 16 S r RNA from three populations. The fixation indices(Fst) analyzed by AMOVA were totaled to Fst=0.0.0090(P0.001) and Fst=0.0674(P0.001), showing that the genetic fixation intra-populations was larger than inter-populations. It indicated that significant genetic fixation existed among the three populations. The Neighbor-joining(NJ) phylogenetic trees were made from the three populations of P. amurensis, showing that the three populations might have originated from one clade, while a small number of individuals may be mixed in otherclades.