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Assay for 3-methoxy-4-hydroxyphenylethylene glycol in human urine by gas chromatography, with electron capture detection.

We describe a gas-chromatographic method, with electron capture detection, for determining 3-methoxy-4-hydroxyphenylethylene glycol in human urine. High sensitivity (5 pg per injection), recovery (93-97%), and reproducibility (CV 4.3%) are obtained. By this method, the 24-h urinary excretion of the free compound, the sulfate conjugate, the glucuronide conjugate, and the total in six normal male subjects average 0.15 (SEM 0.015), 1.09 (SEM 0.10), 1.22 (SEM 0.10), and 2.34 (SEM 0.21) mg, respectively. In three young women with amenorrhea/hirsutism, administration of dexamethasone decreased the total excretion of the compound.