{'fraction_non_alphanumeric': 0.0360250467593722, 'fraction_numerical': 0.03618768805399691, 'mean_word_length': 5.014918072878454, 'pattern_counts': {'":': 0, '<': 8, '<?xml version=': 0, '>': 2, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 1, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Diallyl disulfide induces downregulation and inactivation of cofilin 1 differentiation via the Rac1/ROCK1/LIMK1 pathway in leukemia cells

Cofilin is associated with cell differentiation; however, to the best of our knowledge, no data have indicated an association between the cofilin 1 pathway and leukemia cell differentiation. The present study investigated the involvement of the cofilin 1 signaling pathway in diallyl disulfide (DADS)-induced differentiation and the inhibitory effects on the proliferation, migration, and invasion of human leukemia HL-60 cells. First, it was identified that 8 µM DADS suppressed cell proliferation, migration and invasion, and induced differentiation based on the reduced nitroblue tetrazolium ability and increased CD11b and CD33 expression. DADS significantly downregulated the expression of cofilin 1 and phosphorylated cofilin 1 in HL-60 leukemia cells. Second, it was verified that silencing cofilin 1 markedly promoted 8 µM DADS-induced differentiation and the inhibitory effect on cell proliferation and invasion. Overexpression of cofilin 1 obviously suppressed 8 µM DADS-induced differentiation and the inhibitory effect on cell proliferation and invasion. Third, the present study examined the mechanisms by which 8 µM DADS decreases cofilin 1 expression and activation. The results revealed that 8 µM DADS inhibited the mRNA and protein expression of Rac1, Rho-associated protein kinase 1 (ROCK1) and LIM domain kinase 1 (LIMK1) as well as the phosphorylation of LIMK1 in HL-60 cells, while 8 µM DADS enhanced the effects of the Rac1-ROCK1-LIMK1 pathway in cells overexpressing cofilin 1 compared with that in control HL-60 cells. These results suggest that the anticancer function of DADS on HL-60 leukemia cells is regulated by the Rac1-ROCK1-LIMK1-cofilin 1 pathway, indicating that DADS could be a promising anti-leukemia therapeutic compound.


Introduction
Acute myeloid leukemia (AML) is a malignant and aggressive disease that is sensitive to chemotherapy (1). Although current therapeutic strategies provide a reasonable likelihood of achieving complete remission for the majority of patients, the relapse rates and subsequent disease-associated mortality remain unacceptably high (1,2). There were an estimated 21,450 newly diagnosed cases of AML and 10,920 AML-associated mortalities in the USA in 2019 (2). Improvements in chemotherapeutic regimens and supportive care have increased overall survival rates in younger patients (<15 years of age) to >60%; however, 40% ultimately relapse and require salvage therapy (1). Therefore, there is an urgent need to develop a novel therapeutic drug to enhance chemosensitization. Diallyl disulfide (DADS), a major oil-soluble compound derived from garlic, has multi-targeted antitumor activities in diverse cancer types, which results in the induction of cellular processes, including cell cycle arrest, growth inhibition, differentiation and apoptosis (3). Our previous study (4) demonstrated that DADS could induce

Diallyl disulfide induces downregulation and inactivation of cofilin 1 differentiation via the Rac1/ROCK1/LIMK1 pathway in leukemia cells
the differentiation and inhibit the growth of HL-60 cells by increasing the expression of acetylated histone H3, H4 and p21WAF1 in vitro and in vivo. Using high-resolution mass spectrometry, a total of 18 differentially expressed proteins were identified after treatment with DADS, including four upregulated and fourteen downregulated proteins, among which the protein expression of cofilin 1 was downregulated (5).
Cofilin is an actin-binding protein universally present in eukaryotes that has a molecular weight of 21 kD (6). Two mammalian cofilin gene subtypes exist, cofilin 1 and cofilin 2, which encode different proteins. The former is expressed in a variety of tissues except muscle, and the latter is mainly expressed in muscle tissue (7,8). Cofilin can depolymerize actin filaments and regulate the cytoskeleton (9). A basic function of cofilin is to combine with and depolymerize F-actin intracellularly, thereby inhibiting G-actin polymerization and accelerating actin filament dynamic transformation (10). At the same time, cofilin plays an important role in regulating cell movement (11). The cofilin 1 pathway is regarded as a target to suppress the growth of breast cancer cells (12) and also serves an important role in inhibiting epithelial mesenchymal transition in gastric adenocarcinoma cells (13).
Cofilin can be acted upon by two types of kinases, LIM kinases (LIMKs) and testicular protein kinases, which induce cofilin phosphorylation at the Ser3 site, inhibit the binding of cofilin to F-actin and stabilize F-actin. Two types of cofilin dephosphorylate, Slingshot and chronophin, which can dephosphorylate cofilin, thereby causing the depolymerization and removal of F-actin, promoting actin nucleation through Arp 2/3 and inducing other actin polymerization events and directional movement (14). Cofilin 1 helps regulate glioblastoma cell migration by modulating the cytoskeleton via multiple targets, including F-actin regulation and RhoGTPase activity, such as small Rho-GTPases RhoA and Rac1 activity (15). LIMK1 positively controls the expression and phosphorylation of cofilin 1, leading to rearrangement of the cellular actin cytoskeleton, which serves roles in the growth and motility inhibition of breast cancer cells (12).
Cofilin is associated with cell differentiation in vivo and outside factors can control cofilin through relative cell signaling pathways, thus regulating the actin cytoskeleton to induce differentiation (16). Despite this information, no data have indicated an association between the cofilin 1 pathway and leukemia cell differentiation. The present study aimed to clarify whether downregulation and inactivation of cofilin 1 by DADS induces differentiation. In addition, the mechanism underlying the inhibitory effects on the proliferation, migration and invasion of human leukemia HL-60 cells, and the mechanisms by which DADS mediates cofilin 1 downregulation and inactivation were investigated.
Cell proliferation analysis. The effect of DADS or cofilin 1 on HL-60 cell proliferation was measured using the CCK-8 assay. Briefly, 4x10 3 cells were treated with 0.1% Tween-80 (control) or DADS (8 µM) at room temperature for 24, 48 and 72 h after being transfected or not and then exposed to CCK-8 (10 µl/well) for 4 h. The optical density (OD) values were measured at 490 nm, and the inhibitory rate = (1 -OD value of experience group/OD value of control group) x 100%. Each assay was performed with six replicates.
Western blot analysis. HL-60 cell lines were treated with or without DADS (8 µM) at 37˚C for 12, 24 and 48 h after being transfected or not. Total protein was then extracted using lysis buffer (catalog no. P0013; Beyotime Institute of Biotechnology) and 100 µg/ml PMSF (catalog no. ST505; Beyotime Institute of Biotechnology, China), and quantified with a BCA protein assay kit. Following electrophoresis, total protein (20-25 µg/lane) was separated by 12% SDS-PAGE and transferred onto a PVDF membrane. Primary antibodies, including anti-cofilin 1, anti-Rac1, anti-ROCK1, anti-LIMK1, anti-p-cofilin 1, anti-p-T508-LIMK1 and anti-β-actin, were used at 1:1,000, and incubated overnight at 4˚C. β-actin was employed as the loading control. The blots were washed three times for 5 min in TBS-T (containing 1% Tween-20) and then incubated with peroxidase-conjugated secondary antibody (1:1,000) for 1 h at room temperature. Subsequently, the blots were washed three times for 10 min in TBS-T and then developed using a BeyoECL Plus Hypersensitive ECL Chemiluminescence kit. Alpha Imager 2200 (version 5.0; Alpha Innotech Corporation) was applied to evaluate the ODs of the band densities.
NBT reduction assay. HL-60 cells were maintained at a logarithmic growth rate and seeded at a density of 1x10 4 cells/well. Following exposure to 8 µM DADS at 37˚C for 72 h, cells were collected by centrifugation at 201 x g at room temperature for 5 min. Differentiation of HL-60 cells was assayed by adding 200 µl NBT-TPA solution (2 mg/ml NBT and 0.24 mg/ml phorbol 12-myristate 13-acetate) in PBS to each well, incubation for 1 h at 37˚C, and suspension in 0.4 ml cold 2 M HCl. Subsequently, the formazan product was obtained by centrifugation at 700 x g at room temperature for 10 min, and 200 µl DMSO was added to dissolve the product. The absorbance of the solution was measured at 570 nm.
Immunofluorescence of CD11b and CD33. Immunofluorescence analysis was performed to verify the subcellular localization of the general myeloid differentiation markers CD11b and CD33. HL-60 cells of each group were collected by centrifugation at 201 x g at room temperature for 5 min. Cell suspension was added to cover the slides and dried at room temperature for 30 min. Subsequently, the cells were fixed with 4% polyoxymethylene at room temperature for 30 min, rinsed with 0.5% Triton X-100 in PBS for 15 min, and then blocked with goat serum at room temperature for 30 min. The CD11b (1:100) and CD33 (1:100) antibodies were incubated with the cells at 4˚C overnight, followed by 5-min washes in PBS three times. FITC-conjugated anti-rabbit IgG (catalog no. ab6717; Abcam; 1:1,000) at room temperature for 1 h was applied to assess CD11b and CD33. Images were obtained using an inverted fluorescence microscope (Olympus Corporation; magnification x400) and assessed with PearlScope software (version 1.0.0.1202).
Cell migration and invasion assays. Cell migration and invasion were assessed as previously described (17). Invasion assays were performed using Transwell assays. For the migration assay, cells were resuspended in serum-free RPMI-1640 medium and 100 µl cell suspension (1.0x10 6 cells) was seeded into the upper chambers. RPMI-1640 (500 µl) containing 10% FBS was added to the lower chambers. After transfected and untransfected cells were exposed to 8 µM DADS at 37˚C for 24 h, hematoxylin was used to stain the cells at room temperature for 30 min on the lower surface of the filter. The cells were observed under an inverted microscope (Olympus Corporation; magnification, x400). The invasion assay protocol was the same as that of the migration assay except that the upper chambers were first coated with 1 mg/ml Matrigel. Invasion and migration rates are presented as ratios between DADS-treated and control group values.
Statistical analysis. All results are presented as the mean ± standard deviation for three independent experiments. Comparisons between groups were made by one-way ANOVA with Fisher's Least Significant Difference for three groups and Tukey's test for >3 groups. Statistical analyses were performed using SPSS 13.0 software (SPSS, Inc.). P<0.05 was considered to indicate a statistically significant difference.

Results
Effect of DADS on the differentiation, migration and invasion of HL-60 cells. Following incubation with 8 µM DADS for different amounts of time, the proliferative ability of the human leukemic cell line HL-60 was assayed by cell proliferation analysis. Time-dependent cytotoxicity was determined after 8 µM DADS treatment, and the OD value of the treated cells was 0.311±0.012 at 24 h, 0.362±0.011 at 48 h and 0.389±0.020 at 72 h, which were significantly lower than those of 0.540±0.009 at 24 h, 0.691±0.014 at 48 h and 0.791±0.019 at 72 h in the untreated groups, respectively (Table I).
As presented in Table II, the NBT reduction ability of the untreated HL-60 cells was 0.419±0.010 at 24 h, 0.446±0.016 at 48 h and 0.504±0.026 at 72 h, and following treatment with 8 µM DADS, the NBT reduction ability increased to 0.472±0.023 at 24 h (P=0.003), 0.536±0.006 at 48 h (P=0.001) and 0.620±0.013 at 72 h (P<0.001). Immunofluorescence experiments demonstrated that the expression of CD11b was markedly increased, while CD33 was markedly decreased, in the DADS-treated group compared with the untreated group (Fig. 1A). These results suggest that DADS can induce HL-60 cell differentiation. Cells were exposed to 8 µM DADS for 12 and 24 h, and the effects of DADS on the migration and invasion of the leukemia HL-60 cell line were detected using Transwell experiments. As shown in Fig. 1B, the migration and invasion abilities of HL-60 cells were decreased by 8 µM DADS treatment for 12 and 24 h compared with the control cells (migration at 12 h, P=0.001; migration at 24 h, P<0.001; invasion at 12 h, P<0.001; invasion at 24 h, P<0.001). These data demonstrate that DADS can inhibit the migration and invasion of HL-60 cells.

DADS downregulates Rac1/ROCK1/LIMK1/cofilin 1 signaling in HL-60 cells.
Cofilin is reportedly associated with cell differentiation (16); however, no evidence of an association between the cofilin 1 pathway and leukemia cell differentiation exists. Therefore, the present investigated whether DADS affects the expression and activation of cofilin 1 in leukemia HL-60 cells. As presented in Fig. 2, following treatment with 8 µM DADS for 12, 24 and 48 h, the mRNA and protein expression levels of cofilin 1 in HL-60 cells were significantly inhibited in a time-dependent manner. Similarly, the mRNA and protein expression levels of Rac1, Rock1 and LIMK1 were also significantly lower in HL-60 cells following exposure to DADS for 12, 24 or 48 h. Additionally, both the phosphorylated/total cofilin 1 protein ratio and the phosphorylated LIMK1/total LIMK1 protein ratio significantly decreased at 12 h, 24 and 48 h after 8 µM DADS treatment compared with the control.

Effect of DADS on the proliferation, differentiation and invasion of cofilin 1-silenced HL-60 cells.
Cell proliferation analysis indicated that the proliferation inhibitory effect in cofilin 1-silenced cells (cofilin-miR) was significantly enhanced by treatment with 8 µM DADS for 12, 24 and 48 h (Fig. 3A). However, 8 µM DADS could significantly increase the NBT reduction ability in cofilin 1-miR cells, demonstrating that DADS cooperates with cofilin 1 silencing to affect the differentiation of HL-60 cells (Fig. 3B).
As shown in Fig. 3C, the numbers of cancer cells that migrated and invaded in the Transwell assay were significantly decreased following silencing of cofilin 1 compared with the control vector cells. Furthermore, DADS could significantly strengthen this inhibitory effect, suggesting that DADS negatively effects the invasive ability of HL-60 cells via the downregulation of cofilin 1 expression.
Silencing of cofilin 1 enhanced CD11b expression and inhibited CD33 expression. In addition, the expression of CD11b increased, while the expression of CD33 decreased in the DADS-treated cofilin 1-silenced cells compared with the untreated cofilin 1-miR cells (Fig. 3D).
Effects of DADS on the Rac1-ROCK1-LIMK1 signaling pathway in cofilin 1-silenced HL-60 cells. To clarify the molecular mechanisms by which DADS induces differentiation and exerts inhibitory effects, the present study assessed whether silencing the cofilin 1 gene could interfere with the effects of DADS on the Rac1-ROCK1-LIMK1 signaling pathway. Silencing cofilin 1 significantly downregulated the protein expression of Rac1, ROCK1 and LIMK1 compared with control cells, and significantly increased the inhibitory effect of DADS on the protein expression of cofilin 1, Rac1, ROCK1 and LIMK1 compared with in the untreated cells (Fig. 4).
Effects of DADS on the proliferation, differentiation and invasion of cofilin 1-overexpressing HL-60 cells. Next, it was observed whether cofilin 1 overexpression influences the effects of DADS on proliferation, differentiation and invasion. As presented in Fig. 5A, following 24 h of DADS treatment, the proliferative abilities of the cells in the untransfected, negative control and high cofilin 1 expression groups were significantly lower compared with that in the untreated groups. The difference of the proliferative abilities between the cofilin-overexpression group and untransfected group Table I. Effect of DADS on the proliferation ability of HL-60 cells.      Fig. 5C). Immunofluorescence experiments demonstrated that overexpression of cofilin 1 decreased the expression of CD11b, while the expression of CD33 had no obvious change, compared with the untreated HL-60 cells (Fig. 5D). CD11b expression was increased and CD33 expression was decreased in the DADS-treated cofilin 1-overexpressing cells compared with the cofilin 1-overexpressing cells without DADS treatment (Fig. 5D).  Cell migration and invasion were determined using Transwell migration and invasion assays, respectively. The migration rate is presented as the ratio of migrated cells between treated and untreated HL-60 cells. The invasion rate is presented as the ratio of the mean cell numbers between treated and untreated cells. * P<0.05 vs. the untreated cofilin 1-miR group. $ P<0.05 vs. HL-60 cells (D) Expression of the cell surface differentiation markers CD11b and CD33 following DADS treatment and cofilin 1 miRNA expression plasmid transfection. Magnification, x200. CD11b and CD33 were identified by immunofluorescence (red, left panel). The DNA-intercalating dye DAPI was used to identify cell nuclei (blue, center panel). The right panel displays a merged image to highlight the nuclear pool of CD11b and CD33. Data are presented at the mean ± standard error or the mean from three independent experiments. DADS, diallyl disulfide; miRNA, microRNA; NBT, nitroblue tetrazolium; OD, optical density; cofilin 1-miR, cells transfected with cofilin 1 miRNA. DADS significantly lowered the mRNA and protein expression levels of cofilin 1, Rac1, ROCK1 and LIMK1, and the ratios of p-cofilin/cofilin 1 and p-LIMK1/LIMK1 in cofilin 1-overexpressing cells compared with those without DADS treatment (Fig. 6).

Discussion
DADS, the main anticancer component in garlic, can suppress the proliferation of numerous types of cancer cells, such as leukemia, colon cancer and gastric cancer cells (4,5,18,19). It has been suggested that DADS induces the differentiation of the human gastric cancer cell line MGC803 (20) and the leukemia cell line HL-60 (5). Previously, we demonstrated that 8 µM DADS could induce the differentiation of human leukemia HL-60 cells to a granulocytic lineage, which is similar to the effects of 1 µM all-trans retinoic acid (ATRA) (5). In the present study, DADS could inhibit the proliferation, migration and invasion of HL-60 cells and induce their differentiation, similar to ATRA. These results suggest that DADS may be a potential compound for inducing differentiation. However, the precise molecular mechanisms underlying these anti-metastatic effects of DADS are not completely understood.
In a previous study, DADS was shown to regulate the expression of cofilin 1 (5). The present study verified that DADS gradually decreased the mRNA and protein expression, as well as the phosphorylation of cofilin 1 in HL-60 cells in a time-dependent manner. Cofilin-1, an actin depolymerizing factor (ADF)/cofilin superfamily member, has been shown to be highly expressed in a number of cancer types and is associated with proliferation, migration, invasion, differentiation, metastasis and poor prognosis (16,21). The expression level of cofilin and its subcellular distribution play important roles in the high migratory ability of tumor cells (22). The high expression of cofilin-1 in malignant cells can enhance the speed of cell migration, while silencing cofilin-1 can delay cancer cell metastasis (15). Cofilin-1 can depolymerize actin filaments by binding directly to actin and make the actin filaments turn over quickly (23,24), thus controlling cell movement, which serves an important role in the proliferation and migration of tumor cells (11). Cofilin 1 is involved in tumor invasion, metastasis and prognosis by regulating actin reorganization and pseudopodia formation; sustained activation of cofilin 1 promotes the formation and migration of filamentous pseudopodia in prostate cancer cells and enhances the metastatic ability of prostate cancer in vivo (25). O-(link)-N-acetylation glycosylation modification of the cofilin 1 Ser108 site facilitates its correct localization in the invasive pseudopod and promotes the invasion of cancer cells (26). The high expression and phosphorylation of cofilin 1 serves an important role in the occurrence and development of cancer (27). The suppression of cofilin-1 and the translocation of cofilin 1     from the cytoplasm to mitochondria can induce cancer cell apoptosis (28,29). High cofilin-1 expression is associated with cisplatin chemoresistance in carcinomas (30). Serum cofilin-1 protein expression is increased in carcinomas and may be a promising serum biomarker for prognosis (31,32). Notably, the present study found that silencing cofilin 1 by miRNA could markedly promote the DADS-induced differentiation and inhibitory effect on the proliferation and invasion of HL-60 cells. However, overexpression of cofilin 1 significantly suppressed these effects induced by DADS. These data provide evidence that cofilin 1 is involved in DADS-induced differentiation and growth inhibition in human leukemia HL-60 cells.
Our previous study demonstrated that DADS reduces LIMK1 expression in gastric cancer MGC-803 cells (19) and inhibits the migration and invasion capabilities of colon cancer SW480 cells via downregulation of the Rac1-ROCK1/ PA K1-LI M K1-A DF/cofilin signaling pathway (33). Thereafter, it was suspected that the Rac1-ROCK1/LIMK1 pathway is involved in regulating cofilin expression and activation, and consequently, differentiation  induction in HL-60 cells. In the present study, it was identified that DADS can suppress the mRNA and protein expression of Rac1, ROCK1 and LIMK1, as well as the phosphorylation of LIMK1 in HL-60 cells in a time-dependent manner. Silencing cofilin 1 by miRNA decreased the protein expression of Rac1, ROCK1 and LIMK1, and increased the inhibitory effect of DADS on cofilin 1 mRNA expression and the protein expression of cofilin 1, Rac1, ROCK1 and LIMK1. By contrast, high expression of cofilin 1 reduced the inhibitory effect of DADS on these molecules. Cofilin is regulated by various upstream signals, predominantly RhoGTP enzyme family members, which are involved in tumor occurrence and development. RhoGTP enzyme family members, including Rho, Rac and Cdc42, are closely associated with cytoskeleton reorganization and serve an important role in cell motility, migration and invasion (34). The degree of Rac signaling plays a crucial role in the balance between differentiation and proliferation; cellular Rac1 is indispensable for differentiation (35), and RhoA/ROCK signaling plays an important role in differentiation induction (36). The activation of Rho and Rac1 can phosphorylate the kinase ROCK and activate LIMK1, as Rac can activate LIMK1, which induces cofilin 1 phosphorylation at Ser3 and thus regulates the actin cytoskeleton; this process indicates the formation of the Rac1-ROCK1/LIMK1-cofilin signaling pathway by regulating tumor cell migration and invasion (37,38). The inhibition of the ROCK/PTEN pathway and cofilin-1 expression is involved in the induction of cancer cell apoptosis (39). Cofilin cuts fibrous type F-actin and accelerates free actin polymerization, and the phosphorylated state of cofilin 1 is regulated by LIMK1 (40). In addition, LIMK1-mediated cofilin phosphorylation has important effects on tumorigenesis, matrix adhesion, transfer speed and direction of tumor cell invasion (41). In the present study, the protein expression levels of Rac1, ROCK1 and LIMK1 increased in cofilin 1-silenced HL-60 cells, while the expressions of these molecules were not significantly altered in cofilin 1-overexpressing HL-60 cells. This indicates that there may be a signal interaction centered on cofilin 1, which participates in other signal pathways, such as the Rac1-WAVE2-Arp2/3 pathway (42), when activated, and this conclusion needs to be confirmed by further research in the future. However, DADS can regulate the expression of Rac1, Rock1, LIMK1 and cofilin 1, whether cofilin 1 is overexpressed or silenced.
In summary, DADS could decrease cofilin 1 expression and suppress its phosphorylation by negatively regulating the Rac1-ROCK1-LIMK1 signaling pathway and lead to the inhibited proliferation and induced differentiation of leukemia HL-60 cells (Fig. 7). The present study helps to identify targets for inducing leukemia differentiation and provides a theoretical basis for leukemia differentiation induction therapy.