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Deoxyribonucleoside-triphosphate pools and DNA synthesis in synchronized hamster cells.

Cellular pools of all four deoxyribonucleoside triphosphates were determined in synchronized Chinese hamster cells obtained by mitotic selection or by the addition of complete medium to isoleucine-starved cells. Using either technique the degree of synchrony is high and a maximum of more than 80% of the cells incorporated labeled thymidine during a short pulse period. 
 
 
 
Cells not involved in DNA synthesis contained measurable pools of deoxyribonucleoside triphosphates, but the pools expanded when the cells entered S phase. At all time points the pool of dGTP was the largest while the dGTP pool was the smallest amounting to 1–5% of the size of the dCTP pool. The size of the dCTP pool was closely correlated to the rate of DNA synthesis while the other pools reached their highest values after DNA synthesis.