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GLC assay method for neomycin in petrolatum-based ointments.

A GLC assay method is described for neomycin in various petrolatum-based ophthalmic and topical ointments. Other components such as bacitracin, polymyxin, and steroids do not interfere significantly in the assay. The ointment is dissolved in chloroform, and the neomycin is removed by centrifugation. The chloroform layer is discarded, and the neomycin is dissolved in water and freeze dried. The neomycin is then silylated and chromatographed. The relative standard deviation of the method is approximately 2%, with a recovery of neomycin from spiked samples of 98-100%.