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Multicolor Tunable Polymeric Nanoparticle from Tetraphenylethylene-Cage for Temperature Sensing in Living Cells.

It is meaningful but challenging to develop a fluorescent probe for temperature sensing in living cells because it should possess the features of good cytocompatibility, easy read-out and high resolution. Herein, we successfully syn-thesized emissive star-like cage-based organic temperature sensitive polymers that can assemble into nanoparticles in aqueous solution. The obtained nanoparticle can be easily tuned to full-color emission (including white light emission) with a temperature resolution of at least 0.5 °C by encapsu-lating different doses of guest dyes ((4-Dimethylamino-2'-butoxychalcone (DMBC) and Nile Red (NR)) through cas-cade Förster resonance energy transfer (FRET) effect. Moreover, the white light emission polymeric hybrid nano-particles exhibit reversible stimuli-response toward tem-perature, and can be used as probes for temperature sens-ing in live cells through their fluorescent color variation between white and orange emission with good cytocompati-bility.