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[G-protein modulates the inhibition of angiotensin II on BKCa in ECV304].

OBJECTIVE
To investigate the effect of angiotensin II (A II) on maxi-conductance calcium-activated potassium channel(BKCa) activity in ECV304 cell membrane, and the possible involvement of G-protein.


METHODS
Cell-attached configuration of patch clamp technique was employed to record the current of BKCa in ECV304.


RESULTS
10(-7) mol/L angiotensin II inhibited the BKCa activity. Current amplitude and open probability were decreased, open time was shortened and close time was increased. G-protein activation could eliminate the inhibition of A II.


CONCLUSIONS
A II significantly decreased the activity of BKCa in ECV304, which might depolarize the membrane, and the stability of membrane might change. Thus endothelial cell dysfunction might be induced. G-protein activation was involved in the regulation of the above A II inhibition process.