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MOLECULAR CLONING AND EXPRESSION OF THE LUX GENES OFVIBRIO FISCHERI

Vibrio fischeri is a symbiotic marine bacterium and a nonpathogenic member of the Vibrionaceae which produces luminescence by expressing the lux operon. Lux operon encoding luciferase and proteins. In this study, we amplified the chromosomal fragment contains luxG of V. fischeri and V. harveyi, amplified fragment cloned into the pUC18 vector and sequencing to confirmed the fragment. The sub cloning of luxG gene was carried out in the pUC18 expression vector and expression procedures were performed in Escherichia coli strain. As a result, a 2046 bp fragment which contains the whole fragment of luciferase coding genes and intergenic sequences were cloned in pUC18 expression vector. pUC18 luxG recombinant plasmid was confirmed by restriction analysis In this study, cloning of the luciferase coding genes was performed successfully, in which the synthesized construct can be applied as a reporter cassette in prokaryotic systems and as a marker or tag in the manipulation, and the control of gene expression in the fields of research, production, control of microorganism and other biotechnological applications.