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[Production of human insulin from proinsulin by a biological catalysis].

The conversion of porcine and human proinsulins to insulin by enzymatic hydrolysis induced by trypsin and carboxypeptidase B was studied. The conditions of reactions in the presence of trypsin (pure or immobilized in PAAG) and carboxypeptidase B were determined. The possibility of insulin production by the use of immobilized trypsin and carboxypeptidase B for practical purposes was shown.