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Comparative Evaluation of TLC and HPTLC Plates Containing Standard and Enhanced UV Indicators for Efficiency, Resolution, Detection, and Densitometric Quantification Using Fluorescence Quenching

Abstract Thin layer chromatography (TLC) and high performance TLC (HPTLC) commercially precoated silica gel plates, with enhanced brightness ultraviolet (UV)‐indicator, were compared with comparable plates formulated with standard indicators. Caffeine, acetaminophen, and salicylamide were used as the model test compounds. Results showed that the increased UV‐indicator plates had visually brighter backgrounds, however, the limits of detection (LOD) did not improve. Differences among the plates in terms of efficiency, resolution, and sensitivity and linearity of quantification with slit‐scanning densitometry are also reported.