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Identification of starch in milk protein gels using confocal microscopy

Materials and Methods Milk gels were produced containing a variety of starches including a control gel containing no starch (NS), a modified waxy cornstarch (WC), an instant modified cornstarch (IC), waxy rice starch (RS), instant tapioca starch (ITS), and dextrin (D). Samples were fixed in 1.0% osmium tetroxide in milk serum buffer using a Pelco 3470 microwave system for 20 min at power 2, soaked in 0.5% periodic acid (aq), stained in 1.0% Acriflavin HCl (aq), stained in 0.01% Rhodamine B (aq), rinsing in ddH2O three times after each step. Samples were