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Oxidant Stress and Aspirin-Insensitive Thromboxane Biosynthesis in Severe Unstable Angina

BackgroundUnstable angina is associated with enhanced lipid peroxidation and reduced antioxidant defenses. We have previously reported aspirin failure in the suppression of enhanced thromboxane (TX) biosynthesis in a subset of episodes of platelet activation in this setting. We tested the hypothesis that the in vivo formation of the F2-isoprostane 8-iso-prostaglandin (PG)F2&agr;, a bioactive product of arachidonic acid peroxidation, is enhanced in unstable angina and contributes to aspirin-insensitive TX biosynthesis. Methods and ResultsUrine samples were obtained from patients with unstable angina (n=32), stable angina (n=32), or variant angina (n=4) and from 40 healthy subjects for the measurement of immunoreactive 8-iso-PGF2&agr; and 11-dehydro-TXB2. 8-Iso-PGF2&agr; excretion was significantly higher in patients with unstable angina (339±122 pg/mg creatinine) than in matched patients with stable angina (236±83 pg/mg creatinine, P =0.001) and control subjects (192±71 pg/mg creatinine, P <0.0001). In patients with unstable angina, 8-iso-PGF2&agr; was linearly correlated with 11-dehydro-TXB2 excretion (&rgr;=0.721, P <0.0001) and inversely correlated with plasma vitamin E (&rgr;=−0.710, P =0.004). Spontaneous myocardial ischemia in patients with variant angina or ischemia elicited by a stress test in patients with stable angina was not accompanied by any change in 8-iso-PGF2&agr; excretion, thus excluding a role of ischemia per se in the induction of increased F2-isoprostane production. ConclusionsThese findings establish a putative biochemical link between increased oxidant stress and aspirin-insensitive TX biosynthesis in patients with unstable angina and provide a rationale for dose-finding studies of antioxidants in this setting.