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Orbitrap Mass Analyzer – Overview and Applications in Proteomics

The orbitrap mass analyzer is proving itself as a useful addition to a proteomics tool box. The key attributes of this analyzer are accurate mass and high resolution similar to those achievable with FT ICR instrumentation. The basic principles underlying these capabilities, and how they translate into benefits in real‐life proteomics experiments are discussed. The focus is on reviewing examples of protein identification with bottom‐up and top‐down approaches, and detection of post‐translational modifications.